LOCUS       DBNGVR010000001         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4321936, whole genome shotgun sequence.
ACCESSION   DBNGVR010000001 DBNGVR010000000
VERSION     DBNGVR010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4321936"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="AC16A0_00005"
     CDS             complement(<1..486)
                     /locus_tag="AC16A0_00005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3103736.1"
                     /translation="MTCFRILIILLALLTGTASASAKAAAAGPSLVFDPSTGEVLSQD
                     RAGEPWYPASLTKLMTAYIVFDRLKSGKMTLDQKLPVSEIASAQPPSKIGMKPGSSIS
                     TNLALQSLLIYSANDMAYVLAEGAAGTIEAFVREMNATAQMLGMTGTRFANPNGLFDP
                     RQ"
     gene            complement(569..1666)
                     /locus_tag="AC16A0_00010"
     CDS             complement(569..1666)
                     /locus_tag="AC16A0_00010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_function: GO:0016997 - alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR3103737.1"
                     /translation="MTEGARESRTLSRRALVATLAGIAAAGVVAHSATRRDTYRTYCG
                     TLPMMGVPNDLPAAQTGFVFDPHDAPFASCHASTICETGAGLLCAWFGGAAEGAADTL
                     IYLSLLSDAGWSTPKVVAEGRDQGHAMWNPVLWPSPQGDVLLFYKTGPAPARWMGHLL
                     LLDQGGTPVAPPVAMPRGFLGPTKNKPLLLRDGRLLCPSSTEDRDWTVHFEWTANRGL
                     TWEKSAPVASGYRIIQPALLQHGDGRLQALCRSRDGVIAATWSDDGGRSWSAAQATGL
                     PGNNSGIDAMTLRDGRHALVYNPTELCRSPLHLAISDDGLQWRDAVRLDGGWREYSYP
                     SIIQGKDGALHVSYTWRRASIAHARIDLASL"
     gene            complement(1663..>1837)
                     /locus_tag="AC16A0_00015"
     CDS             complement(1663..>1837)
                     /locus_tag="AC16A0_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3103738.1"
                     /translation="RFTTAKELLALTTLPIGDVGASLSYGSHSSFVHAFRRWAATTPT
                     QWRRNSLARHEPK"
BASE COUNT          318 a          571 c          631 g          317 t
ORIGIN      
        1 ctggcgcgga tcgaaaagcc cgttgggatt ggcgaaacgc gtgcccgtca tgccaagcat
       61 ctgcgccgtg gcattcatct cgcgcacgaa ggcttcaatc gtccctgcgg cgccctccgc
      121 cagcacatag gccatgtcgt tcgccgaata gatcagcagc gactgcaatg ccagattcgt
      181 gctgatgctg ctgcccggct tcatgccgat cttgctgggc ggctgcgcgc tggcgatctc
      241 ggagacgggc agtttctgat caagcgtcat cttgccggac ttgagccggt cgaagacgat
      301 gtaggccgtc atcagcttgg tgagcgacgc aggataccac ggttcgccgg cgcgatcctg
      361 cgagaggact tcgccggtgc tcggatcgaa gacaaggctg gggccggcgg ctgcagcctt
      421 ggcgctcgcg ctcgctgtgc cggtgagcag ggccagcagg atgatgagga tacggaaaca
      481 ggtcacgctg cgacactctt cgtcatgcga tccggtaaaa cacaggctgg cgccgggatc
      541 aacacctgcg gagttacgaa ttcaggcctc agagcgatgc gaggtcgatg cgggcatggg
      601 cgatgcttgc cctgcgccat gtatagctca catgcagcgc gccatctttg ccctggatga
      661 tggaaggata gctgtattcg cgccatccgc cgtcgagccg cacggcatcg cgccactgca
      721 gcccgtcgtc cgagatggca agatgcaacg gcgaccggca cagttctgtc gggttgtaga
      781 caagcgcatg ccggccatcc cgcagcgtca tggcatcgat gccgctgttg ttgccgggaa
      841 ggcctgttgc ttgcgcggcc gaccagctgc ggccgccgtc atcggaccat gttgcggcga
      901 tgacgccgtc gcggctgcgg cacagcgcct gcaggcggcc atcgccgtgc tgaagcaggg
      961 cgggctggat gatgcggtag ccgctggcga cgggagccga tttctcccag gtcaggccgc
     1021 gattggccgt ccactcgaag tgtacggtcc agtccctgtc ctcggtgctt gacggacaga
     1081 ggagcctgcc atcgcgaagc agcagcggct tgttcttggt gggcccgagg aatcctcggg
     1141 gcatggcgac cggcggcgcg acgggtgtcc ccccttggtc gagaagcagc agatggccca
     1201 tccagcgcgc gggcgcaggg ccggtcttgt agaagagcag aacatcgccc tgcggcgacg
     1261 gccacagcac cggattccac atcgcgtggc cttggtcgcg gccttcggca acaacctttg
     1321 gagtcgacca ccccgcatcg ctcagcaggc tgagatagat gagggtatcg gccgcacctt
     1381 ccgccgcgcc gccgaaccag gcacagagaa gacctgcgcc ggtctcgcag atggtcgagg
     1441 cgtggcagct cgcgaaggga gcatcgtgag gatcgaagac gaagcccgtc tgcgccgccg
     1501 gcaggtcatt cggcacaccc atcatgggca atgtgccgca gtaggtcctg taggtgtctc
     1561 gccgggttgc ggaatgagcg acgacaccag ccgctgcgat gcccgccagc gtggcgacga
     1621 gcgcacggcg cgatagggtt cggctctcgc gggcaccttc agtcacttcg gctcatgcct
     1681 cgccaggctg ttgcggcgcc attgcgttgg ggtcgtcgcc gcccagcggc ggaaggcgtg
     1741 gacgaaactg ctgtgcgaac cataggacag cgaagcgccc acgtcgccga ttggcagcgt
     1801 ggtgagcgcc aggagttcct tcgcggtggt gaagcgg
//
LOCUS       DBNGVR010000002         2901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4322338, whole genome shotgun sequence.
ACCESSION   DBNGVR010000002 DBNGVR010000000
VERSION     DBNGVR010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4322338"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="AC16A0_00020"
     CDS             <1..1041
                     /locus_tag="AC16A0_00020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3103739.1"
                     /translation="ARKAESAKKGKLRGIGFSNFIEACGIAPSAAVGSLGCGVGLWES
                     AEVRVNPVGTVEVLTGSHSHGQGHETTFAQLVSDRLGIDINSVSIVHGDTDKVQFGMG
                     TYGSRSGAVGMTAIAKALDKIEAKAKKVAAHVMEAAEGDIEFANGSFSVKGTDKKMAF
                     GEVALSAYVAHKFNSAEIEPGLKESAFHDPSNFVFPAGVHICEVEVDADTGVTTVANY
                     VAVDDFGNIINPMIVEGQVHGGIVQGIGQALAEGCVYDKESGQLMTGSYMDYQMPRAA
                     DVPSFKLDFTCTPCPSNPLGMKGCGEAGAIASPAAVMNAVTDALGVRDVPMPATPQVV
                     WKTMQPMKKAAE"
     gene            1060..1854
                     /locus_tag="AC16A0_00025"
     CDS             1060..1854
                     /locus_tag="AC16A0_00025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3103740.1"
                     /translation="MENFNYVRPAKVADAVKAMKKAKDGKYLSGGHTLLPTLKQGLAA
                     PTDIIDLSGLKNAGVKVGAKSVTIGAGTTHAEVAGSKDLKKAIPGLAKLAGGIGDPHV
                     RHRGTIGGSLANNDPAADYPAAALGLGATIHTSDRKIPAEKYFTGMFSTALKGSEIVT
                     AVEFPVPKKSGYAKFPNPASLYAMVGVFVAQLADGSVRVAVTGAGPGVFRAPELEAAL
                     AKNFSAKALDGIAVKAKGLNSDMHASAEYRAHLITVMAKRAVEAAK"
     gene            1926..2846
                     /locus_tag="AC16A0_00030"
     CDS             1926..2846
                     /locus_tag="AC16A0_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3103741.1"
                     /translation="MTQLPTSIDETLTQLRSTGYVAGRDLATVVFLSLRMGRPLFLEG
                     EAGVGKTEIAKVLADVLGRRLIRLQCYEGLDVNSAVYEWNYSAQMIEIRLAEAAGELD
                     RARLGQDIFAERFLIKRPLLQALDAPKGQAPVLLIDELDRTDEAFEAYLLEVLSDFQV
                     TIPELGTIRAEEPPIVIITSNRTREVHDALKRRCLYHWVGYPDARRELEIVKARRPQA
                     SDALAKEVVLFVQALRGGDLYKSPGVAETLDWVSALHELDSLTLDPSTVNDTLGVLLK
                     YQDDISRMQGTEAKRILDQIKDEIRSMGKG"
BASE COUNT          550 a          916 c          946 g          489 t
ORIGIN      
        1 gcccgcaagg cggaatcggc gaagaaaggc aagctgcgcg gcatcggctt ctccaacttc
       61 atcgaggcct gcggcatcgc gccgtcggcg gcggtggggt cgctcggctg cggcgtcggc
      121 ctttgggaat cggcggaagt gcgcgtcaac ccggtcggca ccgttgaggt gctcaccggc
      181 tcgcacagcc acggccaggg ccacgagacg accttcgccc agctcgtttc cgaccgtctc
      241 ggcatcgaca tcaactcggt gtcgatcgtc cacggcgaca ccgacaaggt gcagttcggc
      301 atgggcacct acggttcgcg ctccggcgcg gtgggcatga ccgccatcgc caaggcgctc
      361 gacaagatcg aggccaaggc gaagaaggtc gccgcccacg tcatggaggc ggcagagggc
      421 gacatcgagt tcgccaacgg ctcgttctcg gtgaagggca ccgacaaaaa gatggcgttc
      481 ggcgaagtgg cactgtcggc ctatgttgca cacaagttca actcggcgga aatcgagccg
      541 ggcctcaagg agagtgcctt ccacgacccg tcgaacttcg tgttcccggc cggtgtgcac
      601 atctgcgagg tcgaggtgga tgccgatact ggcgtcacga cggtcgccaa ctacgtggcg
      661 gtggatgact tcggcaacat catcaacccg atgatcgtcg agggccaggt ccatggcggc
      721 atcgtccagg gcatcggaca ggcgcttgcg gaaggctgcg tctacgacaa ggagtccggc
      781 cagctcatga ccggttccta catggactac cagatgccgc gcgcggcaga cgtaccctcg
      841 ttcaagctcg acttcacctg cacgccctgt ccgagcaacc cgctcggcat gaagggctgc
      901 ggcgaagctg gcgccatcgc ctctccggcg gcggtgatga acgcggtgac cgatgcgctc
      961 ggcgtgcgcg acgtgcccat gccggcaacg ccgcaggtgg tctggaagac catgcaaccc
     1021 atgaagaagg ctgcggaata aggttcggga ggacccaaga tggaaaactt caactacgtt
     1081 cgtccggcca aggtggcaga tgccgtcaag gcgatgaaga aggccaagga tggcaagtac
     1141 ctctccggag ggcatacgct gcttccgacg ctgaagcagg ggcttgcggc accgaccgat
     1201 atcattgacc tgtccggcct caagaacgcc ggcgtcaagg tcggcgccaa gtcggtgacc
     1261 atcggcgcgg gcacgaccca tgccgaggtg gccggcagca aggacctcaa gaaggccatt
     1321 cccggccttg ccaagcttgc gggtggaatc ggcgatccgc atgtgcggca ccggggcacg
     1381 atcggcgggt cgctcgccaa caacgacccg gcggccgact atccggccgc tgctctgggc
     1441 ctcggcgcca cgatccatac ctctgaccgc aagatcccgg cagagaagta tttcaccggc
     1501 atgttctcaa ccgccctcaa gggttcggag atcgtcacgg cggtggagtt cccggttccg
     1561 aagaagagcg gctatgccaa gttccccaac ccggcctcgc tctatgccat ggttggcgtc
     1621 ttcgtggcac agctcgcgga cggctcggtc cgcgtggccg tgaccggcgc aggtccgggc
     1681 gtgttccgcg cgcccgaact ggaagcggcg cttgccaaga acttctcggc gaaggcgctg
     1741 gatggcatag cggtcaaggc caagggcctg aacagcgaca tgcatgcctc ggcggaatac
     1801 cgcgcgcatt tgatcacggt catggcgaag cgggccgtgg aagcggcgaa gtaagcttcg
     1861 acgcgcgttt gttacggaac ccgcgggcgc gatctcgcgg gtttttcatt tcgtaaggaa
     1921 gcatcatgac ccagttgccc acatcgatcg acgagaccct cacgcagctg cgctcgactg
     1981 gctacgtggc tgggcgcgat ctggcgacgg tggtgtttct cagcctgcgc atggggcgtc
     2041 cgctgttcct cgaaggcgag gcgggtgtcg gcaagaccga aatcgccaag gtgctggctg
     2101 atgtgctcgg ccgccggctg atccgcctgc agtgttacga gggcctggac gtcaattccg
     2161 ccgtttatga gtggaactac tccgcgcaga tgatcgagat ccggttggca gaagccgcag
     2221 gcgaactcga ccgcgcgcgg ctggggcagg atatctttgc cgaacgtttc ctgatcaagc
     2281 gccccctgct gcaggcgctg gatgcgccga aggggcaggc gccagtgctg ctgatcgatg
     2341 aactcgaccg caccgacgag gctttcgagg cctatctcct ggaggtcctc tccgacttcc
     2401 aggtcaccat cccggagctg ggaaccataa gggccgaaga accgcccatc gtcatcatca
     2461 cctccaaccg cacgcgggaa gtgcatgacg ccctcaaacg ccgctgcctc tatcactggg
     2521 tcggctatcc ggatgccagg cgcgagctgg agatcgtcaa ggcccggcgg ccgcaggcct
     2581 ccgatgcgtt ggcgaaggaa gtcgtgctct ttgtccaggc actgcgcggc ggcgacctct
     2641 acaaatcgcc aggcgtggcc gaaacgctgg attgggtctc tgcgctgcat gaactcgact
     2701 cgctcacgct ggatccctcg accgtcaacg atacgcttgg cgttctgctg aaatatcagg
     2761 acgacatctc acgcatgcag ggcaccgagg cgaagcgcat cctcgaccag atcaaggacg
     2821 agatccgctc catgggcaag gggtgatgtt tccccaggcc gccaatcaga accttgcgcc
     2881 gctctcgggc aagctggccg a
//
LOCUS       DBNGVR010000003         3789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_197915, whole genome shotgun sequence.
ACCESSION   DBNGVR010000003 DBNGVR010000000
VERSION     DBNGVR010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_197915"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="AC16A0_00035"
     CDS             complement(<1..332)
                     /locus_tag="AC16A0_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3103742.1"
                     /translation="MRKSYLDYAMSVIVSRALPDVRDGLKPVHRRILYSMHENGYDWN
                     KPYRKSARIVGDVMGKYHPHGDQSIYDALVRMAQDFSLRLPLIDGQGNFGSVDGDPPA
                     AMRYTESR"
     gene            complement(586..1071)
                     /gene="ssb"
                     /locus_tag="AC16A0_00040"
     CDS             complement(586..1071)
                     /gene="ssb"
                     /locus_tag="AC16A0_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3103743.1"
                     /translation="MAGSVNKVILVGNLGRDPEVRHTQDGKSIVNLSLATSESWRDKS
                     TGERKEKTEWHRVVIFNEALAKVAEQYLKKGSTVYIEGQLQTRKYTDKDGVEKYSTEV
                     VLQNYRGELTMLGGRAGGEGAGGFGGGDDFGQSSPMERPRSGAAKPQSFTRDLDDEVP
                     F"
     gene            1213..1536
                     /locus_tag="AC16A0_00045"
     CDS             1213..1536
                     /locus_tag="AC16A0_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3103744.1"
                     /translation="MLKTIDTPNAPRPFSNYAQGIAVAPGAWLLFVSGQVGATVGGDI
                     PEDARRQHQLAWENVLAILAAEGLDHRHVVDAHVYITDRDHVGLYREVRDRMLMGNKR
                     LRLHF"
     gene            1891..2838
                     /gene="gcvA"
                     /locus_tag="AC16A0_00050"
     CDS             1891..2838
                     /gene="gcvA"
                     /locus_tag="AC16A0_00050"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HIR3103745.1"
                     /translation="MARRLPSLNALKAFEAAARHESFTDAAAELFVTHAAISRHIRDL
                     EEWLGTELFLRTGRGVTLTEAGRRFGERLTPLFDAIAEATREAAAAGDARQLRVSIEP
                     AFASRWLVPRLGRFNELHPDIELAIDPNSKLADFRSGEADLGLRYGPGGWDDVEALKL
                     TDTVIFPVASPDLLADHKSLKPGDLHDFNLLHESRKQWWTDWLAAAGVSGVEDWRGTV
                     FQNHLAIEAAEAGQGFALGDQILCTDSLIEGWLVRPFDLDMRDHGTYWVVRARGSKET
                     PPARAFREWVMAEMADTNRKYAQIKAQRSATVAGKAPTA"
     gene            2877..3254
                     /locus_tag="AC16A0_00055"
     CDS             2877..3254
                     /locus_tag="AC16A0_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR3103746.1"
                     /translation="MPAKKYDCLKCPGYCCSYPVIEVKDRDAERIAKHFGLSLKAAEK
                     KYFKSAHGYKRVMRRKADEHFGKICQFFDTTERRCTIYLARPSTCRVFPGEGRCGYYD
                     FLKFERAGQEDETYISTTYHSEN"
     gene            complement(3243..>3789)
                     /locus_tag="AC16A0_00060"
     CDS             complement(3243..>3789)
                     /locus_tag="AC16A0_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020939.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HIR3103747.1"
                     /translation="SGKANEKAVAFRHVDHAVFGIGRDRPRTEWQSLIRQLVGGGFLA
                     IDVAGYGGLSLTPKARALMAGDGQFRYRRERVRGTKKVERQRRIAVAVADEGVSEELL
                     QRLKALRARLARQGQVPAYVVFSDRSLIDMARRVPLTRWDFGEVHGVGEAKLERFGEV
                     FLAEVRRYVEEGAATRRASVL"
BASE COUNT          685 a         1254 c         1154 g          696 t
ORIGIN      
        1 atgcgcgact ccgtgtagcg catggcggcc ggcggatcgc cgtcgaccga gccgaagttg
       61 ccctggccat cgatcagcgg caggcgcagc gaaaagtcct gcgccatgcg gacgagcgcg
      121 tcatagatcg actggtcgcc atgcggatgg tacttaccca tgacgtcgcc gacgatgcgc
      181 gccgacttgc gatagggctt gttccagtcg tagccgttct cgtgcatcga atagaggatg
      241 cgccggtgca ccggcttcaa cccgtcgcga acatccggaa gcgcgcggct cacgatcacg
      301 ctcatggcgt aatcgaggta gctcttgcgc atctcctctt cgatggagac gggagaaatc
      361 tcggatggat tggcggggtt caggccaccc ttcttgtcgt cgtcatcggc cacggaaaac
      421 ctgcaagaac actgcggaca agcctgccgg gcaacgccgg aacgggacga ttcggaaggc
      481 ctcccagact agttcagggg ccttccggat acaatgaaaa cacgccgcaa ccggactgaa
      541 aaagggcccc ggaagggccc ttgaaaacac tttcagatca gagtcttaga atggaacttc
      601 gtcgtccagg tcacgggtga agctctgcgg ctttgccgcc ccgctgcgcg gccgttccat
      661 cgggctcgac tggccgaaat catcgccgcc gccgaagccg cccgcgccct ctccgccagc
      721 ccgtccgccg agcatcgtca gctcgccgcg gtagttctgc aggaccactt cggtggagta
      781 cttctcgacg ccgtccttgt cggtgtattt gcgggtctgc agctggccct cgatgtagac
      841 cgtcgatccc ttcttgagat actgctcggc caccttggcc agcgcttcgt tgaagatgac
      901 gacgcgatgc cactcggtct tctccttgcg ctcgccggtg gacttgtcgc gccaggattc
      961 ggaggtggca aggctgaggt tcacgatgga cttgccgtcc tgggtgtgac ggacctccgg
     1021 atcgcgaccc agattgccca cgagaatgac tttgttcacc gacccggcca tgaccgatct
     1081 cctctgattt cgcgcaccct agtcaatggt ccgcaccgac acacttgcgg atggcgtctt
     1141 atccacacgt gttagatagt tctctttttg ttctcacgca aggggcctag gatcgcgcaa
     1201 agggagaaca ccatgctgaa aaccatcgac acgcccaatg cgccgcgtcc cttctcgaac
     1261 tatgcccagg gcattgccgt ggcgccgggc gcgtggctgc tcttcgtttc gggacaggtg
     1321 ggggccacgg tcgggggtga catcccggaa gatgcccggc gccagcacca gctggcctgg
     1381 gagaatgtcc tggccatcct cgcggccgag gggctcgatc atcgccatgt ggtggatgcc
     1441 cacgtctaca tcacggatcg cgatcacgtg ggcctctacc gcgaggtccg ggaccggatg
     1501 ctgatgggaa acaagcgcct gcggctacac ttctgattgt gtcaggcctg gcagacccgc
     1561 ggctcgtcgt ggaggttggg gttgtggcag cggccccggc cacatgagcc ggagccgctg
     1621 aaaatcgtat cgcttagcgg cggccgaaga gcccgaaaat ggaagtccgc ttggcattgg
     1681 gccgctcctt cgcagttgca gcattgtgtt cggcagactt tgtctgcatc atggcggcac
     1741 cgtaaacatt tgaataagtt tcaaaaaccc agttcatgtt gaaatctcca taagttgctt
     1801 cgtgttgtcc cctatatgcc tgcttcgctc ttgcaacaca aacggcaata acctacgctt
     1861 ggcgtaacaa aaccttacag gacagaaacc ttggctcgcc gcctgccctc cctcaacgcc
     1921 ctgaaggctt tcgaagccgc cgcccggcac gagagcttca cggatgccgc tgcggagctc
     1981 ttcgtgaccc acgccgccat cagccgtcat atccgcgatc tcgaggaatg gctgggcacc
     2041 gagctgttcc tgcgtaccgg ccggggcgtg accctgaccg aagccgggcg gcgcttcggc
     2101 gagcgcctca cacccctttt cgacgcgatc gccgaagcca cgcgggaagc ggctgcggcg
     2161 ggcgacgcgc ggcagcttcg ggtttccatc gagcccgcct ttgcgtcgcg ctggctggtg
     2221 ccgcgccttg gccgcttcaa cgagcttcac cccgacatcg agctggccat cgaccccaac
     2281 agcaagctcg ccgacttccg ctccggcgag gccgacctcg gcctgcgcta tggcccgggc
     2341 ggctgggacg acgtggaggc cctcaagctt accgacacgg tgatctttcc cgtcgcctcg
     2401 cccgatcttc tggcggacca caagagcctc aagcccggcg atctccatga cttcaacctg
     2461 ctgcatgaaa gccgcaagca gtggtggacc gactggctgg ccgccgccgg cgtcagcggc
     2521 gtggaggact ggcgcggcac cgtgttccag aaccacctgg ccatcgaggc tgcagaggcc
     2581 ggccagggct tcgccctggg cgatcagatc ctttgcaccg actcgctcat cgagggctgg
     2641 ctggtgcggc ccttcgatct cgacatgcgc gatcacggca cctactgggt ggtgcgcgcc
     2701 aggggctcca aggaaacccc gccggcccgc gccttccgtg aatgggtcat ggccgagatg
     2761 gcggacacca acaggaaata tgcacagatc aaggcgcagc gcagcgcgac cgttgcgggc
     2821 aaggctccga ccgcctagcg ttccgggctt catctaccag acaaagagat tcgccgatgc
     2881 ccgccaagaa gtatgattgc ctgaagtgtc ccggctattg ctgttcctat ccggtgatcg
     2941 aagtgaagga ccgcgacgcc gaacgcattg ccaagcactt cggcctcagc ctcaaggcgg
     3001 cggagaagaa gtatttcaag tcggcgcatg gctacaagcg cgtcatgcgg cgcaaggccg
     3061 acgagcattt cggcaagatc tgccagttct tcgatacgac cgagcggcgc tgcacgatct
     3121 atctggcgcg tccctcgacc tgtcgcgtct ttccgggcga aggccgctgc ggctactacg
     3181 acttcctgaa gttcgagcgc gccggccagg aagacgagac ctacatctcg acgacctatc
     3241 actcagagaa ctgaggcccg ccgcgtcgcg gccccttctt ccacatagcg ccgcacctcc
     3301 gcgaggaaga cttcgccgaa gcgttcgagc ttggcttcgc ccacgccatg gacttcgccg
     3361 aagtcccagc gggtgagcgg cacccgccgc gccatgtcga tgaggctgcg gtcggagaag
     3421 accacatagg cgggcacctg gccttgccgg gccagcctgg cgcgcagcgc cttcagcctt
     3481 tgcagcagct cttccgaaac tccttcgtcg gcgacggcca ctgcgatgcg gcgctggcgc
     3541 tcgaccttct tcgtgccacg cacccgttcg cgccggtagc ggaactgccc gtcgcctgcc
     3601 atcagcgccc gcgccttggg cgtgagggaa agcccgccat agcccgccac gtcgatggca
     3661 aggaatccgc cacccaccag ttgccggatc agggactgcc actcggtgcg cggacgatcc
     3721 ctgccgatgc cgaagaccgc gtgatcgaca tgacggaagg ccaccgcctt ctcgttggcc
     3781 ttgccggac
//
LOCUS       DBNGVR010000004         4839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2949300, whole genome shotgun sequence.
ACCESSION   DBNGVR010000004 DBNGVR010000000
VERSION     DBNGVR010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2949300"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC16A0_00065"
     CDS             complement(<1..695)
                     /locus_tag="AC16A0_00065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3103748.1"
                     /translation="MPKQPIVIIGAGVGGLSAAALLAARGEPVLVLERAATPGGKMRQ
                     VKIGDAAIDGGPTVFTMRWVFDDIFAAAGSRLEDHLALARLDILARHAWGPSQRLDLF
                     ADVRRSADAVASFSGAEEGRRFLSFCAESRRIYDTLRKPFLMSQRPDPVKLATANGLG
                     GMTGMLRINPFEKMWTALGRHFTDPRLRQLFGRYATYCGSSPFEAPATLMLVAHVEQE
                     GVWAVAGGMHRLAL"
     gene            805..1674
                     /locus_tag="AC16A0_00070"
     CDS             805..1674
                     /locus_tag="AC16A0_00070"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3103749.1"
                     /translation="MDIAQRLEQELEQAVTRATGPECPPRLAEALHYAVFPGGARIRP
                     RLCLAVARACGDSNFVAATAAAAAIELLHCASLVHDDLPCFDNAGIRRGKPSVHCAFG
                     ERTAVLAGDALIVLAFEQLAIRLALSPDRLAPITRIVAAAVGGPNGICAGQAWENEDK
                     IDIVNYQRAKTGALFAACTMAGAAAAGLEPQAWQTLGYAIGEAFQVADDLRDVAGQAQ
                     DLGKPTGQDAANHCPNFVDELGFDGAMQRFEDHLAAALAAIPPCPGAEQLGLQITAVS
                     RSLVTGLSARQAA"
     gene            1671..2792
                     /locus_tag="AC16A0_00075"
     CDS             1671..2792
                     /locus_tag="AC16A0_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3103750.1"
                     /translation="MSLLGSLGDRWLNLRNRLLANPRFQDLATRFPLTRPISRRRSKD
                     LFDLLAGFTYSQVLYAVVRLNLLESLRQGPLETADIARRIGWPADRTGRLLKAAAAID
                     LLEVTSTGAYGLGIHGAALLGNPWIGRFVEHHHLLYEDLADPLALLRGQRTDTSLRRF
                     WAYAGSASPETARPEETSAYTQLMAASQQAVAGEILAAYDFGQHRHLMDVGGSNGTFI
                     ARAAARHPALALTLFDLPGVISHARDSDAIRALGDRLSVHGGSFLSDPLPQGADVITL
                     IRILHDHDDESVLKLLKAVRAALAPGGKLVVAEPFSGLKSTAAVTDAYFDLYFTAMGQ
                     GRTRTVAEIGRIASTAGFAAIRPVPTRMPLITGLAVLST"
     gene            2910..3848
                     /gene="bchC"
                     /locus_tag="AC16A0_00080"
     CDS             2910..3848
                     /gene="bchC"
                     /locus_tag="AC16A0_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0036354 - bacteriochlorophyllide-a
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthesis pathway protein BchC"
                     /protein_id="HIR3103751.1"
                     /translation="MKTVAVVLEKPENLGLRHIDLAPASAGDVVVQTEWSGISSGTER
                     LLWSGRMPSFPGMGYPLVPGYETVGRVISAGASARLRPGDEVFVPGANCYGEIKGLFG
                     GAASRIVVPEHRTVALPAGLGETGILFALAATAHHAVAGRNAILPELIIGHGILGRLI
                     ARITIALGAEAPTVWERNPMRQDGDGYAVIDPADDARRNYGAICDASGDASLIDTLIG
                     RLAKGGEIAIAGFYETPISFAFPPAFMREARLRIAAEWAAEDIAAVRSLIDGGKLSLD
                     GLITNRVTATDAEAAYRTAFTDPTCLKMVLDWRTIQ"
     gene            3845..>4839
                     /locus_tag="AC16A0_00085"
     CDS             3845..>4839
                     /locus_tag="AC16A0_00085"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase iron protein subunit
                     X"
                     /protein_id="HIR3103752.1"
                     /translation="MNSIDVNLVRQAKLREEAAIPPDPVHTGPVKKETQIIAIYGKGG
                     IGKSFTLANLSYMMAQQGKRVLLIGCDPKSDTTSLLFGGKACPTIIETSARKKLAGEE
                     VKIGDVCFKRDGVFAMELGGPEVGRGCGGRGIIHGFELLEKLGFHEWGFDFVLLDFLG
                     DVVCGGFGLPIARDMCQKVIIVGSNDLQSLYVANNVCSAVTYFRKLGGNVGVAGIVVN
                     KDDNTGEAHAFAEKVGIPVLASIPANEDIRRKSANYEIIGKPGGDWGALFEALALNVA
                     DAPPQHPSPLDQDGLLGLFSGDAVGRGVVLQPASLEDMCGGTIVDRPSLEVIYDTV"
BASE COUNT          851 a         1677 c         1493 g          818 t
ORIGIN      
        1 agggccaggc gatgcatgcc gcccgcaacc gcccagacac cttcctgctc gacatgggca
       61 accagcatca gcgttgccgg agcctcgaag ggcgacgacc cgcaataggt cgcataacgc
      121 ccgaagagct ggcgcagccg cggatcggtg aaatgccggc ccagcgcggt ccacatcttc
      181 tcgaagggat tgatgcgcag catgccggtc atgccgccga ggccgttggc ggtggccagt
      241 ttcaccggat cgggccgctg cgacatcaga aagggcttgc gcagggtgtc gtagatgcgc
      301 cgggactcgg cgcagaagct gaggaaccgg cgcccctctt cggctccgga gaaggacgca
      361 acggcgtcgg ccgagcgccg gacgtcggcg aagagatcga ggcgctggga gggaccccag
      421 gcatggcggg ccagaatgtc gagcctggcc agcgccagat ggtcctcaag cctgctgccg
      481 gccgcagcga agatgtcgtc gaagacccag cgcatggtga agacggtcgg accgccatcg
      541 atcgccgcat ctccgatctt gacctgccgc atcttgccgc cgggggttgc cgcacgctcg
      601 agcacaagaa ccggctcccc gcgggccgcg agcagcgccg ctgcggacaa gccgccgacg
      661 cccgctccga tgatgacgat gggttgcttc ggcaccgccc cgctgatccc tgttgcgcga
      721 cctccgcagt tatgtcagta ttacttgaca cattcaagtg acactcaagt gtgacagtca
      781 cgccgggtac agggagacgc tgacatggac attgcacagc gcttggagca ggagctcgaa
      841 caggccgtga cccgggccac cggacccgag tgcccgccgc gccttgccga ggccctccac
      901 tacgccgtct ttcccggcgg cgcccgcatc cgcccacgcc tctgcctcgc ggtcgccagg
      961 gcctgcgggg attccaactt cgttgccgcc acggccgccg ccgccgccat cgaacttctg
     1021 cactgcgctt cgctggtaca cgacgatctt ccctgcttcg acaacgcagg tatccggcgg
     1081 ggcaaaccgt ccgtacactg cgcattcggc gagcgcaccg cagttctcgc cggcgacgcg
     1141 ctgatcgtat tggccttcga acagctggcg atccggctcg cgctctcgcc cgaccggctg
     1201 gcaccgatca cgcgcatcgt tgccgcggcc gtcgggggcc ccaacgggat ctgcgccgga
     1261 caggcctggg aaaacgaaga caagatcgac atcgtcaact atcagcgcgc caagaccgga
     1321 gcgcttttcg cggcctgcac gatggccggc gctgccgcag ccgggctgga accgcaggcc
     1381 tggcagactc tcggctatgc catcggcgag gccttccagg ttgccgacga cctgcgcgac
     1441 gtcgccgggc aggcgcagga cctgggcaaa cccactggcc aggacgccgc caatcactgc
     1501 ccgaacttcg tcgacgaact tggcttcgac ggcgccatgc agcgcttcga agatcatctt
     1561 gctgccgctc tcgcggccat tcccccctgc cccggcgcgg agcaactcgg cctgcagatc
     1621 acggccgtct cgcgcagcct ggtgaccggc ctgagcgccc gccaggccgc gtgagtctgc
     1681 tcggatccct gggtgaccgc tggctgaacc tgcggaaccg gcttctcgcc aacccgcgct
     1741 ttcaggatct tgccacccgc tttcccctga cacggccgat aagtcgcagg cgatccaagg
     1801 acttattcga tctgctcgcc ggcttcacct attcccaggt gctctatgca gtggttcggc
     1861 tcaaccttct cgagtccttg cgccaagggc cgctcgaaac cgccgacatc gcccgccgaa
     1921 tcggctggcc ggccgaccgg accggacgcc tgctgaaggc cgccgcggcg atcgacctcc
     1981 tcgaagtcac gagcaccggg gcctatggcc tcggcattca tggtgcggca ctcctcggca
     2041 acccctggat cggccgcttc gtcgagcacc accacctcct ctacgaagac ctcgcggacc
     2101 ccctggcgct gctgcgcggc cagcgcaccg acacatccct gcggcgcttc tgggcctatg
     2161 ccggatcagc gtcgcccgaa acggcgcggc ccgaagagac aagcgcctat acgcagctca
     2221 tggccgcatc gcagcaggcc gtagccggcg agatccttgc agcctatgat tttggccagc
     2281 accggcattt gatggacgtg ggcgggagca acggcacctt catcgctcgc gctgccgcgc
     2341 ggcaccccgc acttgcgctc acccttttcg atctccccgg cgtgatcagc cacgcccgcg
     2401 actcggacgc catccgcgcc cttggagaca ggctctccgt tcacggagga tcttttctct
     2461 ccgatcccct tccgcagggg gccgatgtca ttacgctgat ccgcattctc catgaccacg
     2521 acgacgaatc ggtcttgaag ctgctgaagg ccgtcagggc tgccctggcg cctgggggaa
     2581 aacttgtcgt ggccgagccc ttctccggac tgaaatccac cgccgcagtt acggacgcct
     2641 acttcgatct ctatttcacg gccatgggtc agggcaggac ccggacagtg gcagagatcg
     2701 ggcggatcgc atccacggcc ggctttgccg ccatcaggcc ggtcccgaca cgcatgcccc
     2761 tgatcacggg gctggcagtc ctgtcaactt gagctagcgc aaaatatgta catcacagtt
     2821 gacactctct ggtgtcagag taaagtgaca accgggactc tgacaaacct gcccaattca
     2881 gcagcaggag gctcagatgg agaccgacca tgaagacggt cgcagttgtt ttagagaagc
     2941 ctgagaatct ggggctcagg catatcgatc tggctccggc cagcgcgggc gacgtcgtcg
     3001 tgcagacgga atggtcgggc ataagttccg gcaccgagcg cctgctctgg tccggccgca
     3061 tgcccagttt ccctggcatg ggctatcccc tagttcccgg ttacgaaacg gtcggccggg
     3121 tgatcagcgc cggtgcatcg gcgcggcttc ggccgggtga cgaagtcttc gttcccggcg
     3181 ccaactgcta cggcgagatc aaggggctgt ttggcggtgc cgcctcgcgc atcgtggttc
     3241 ccgagcaccg gaccgtggcc cttccggccg gactgggcga gaccggcatc ctcttcgctc
     3301 tggccgccac ggcccatcac gccgtcgccg gaaggaacgc aatccttccg gaactcatca
     3361 tcggccacgg aatactgggc cgcctcattg cgcgcatcac catcgcactg ggtgccgaag
     3421 cgccgaccgt ttgggagcgc aaccccatgc ggcaggacgg cgatggctat gccgtcatcg
     3481 atccagccga cgatgcgcga cggaactacg gtgcaatctg cgacgcaagt ggcgatgcgt
     3541 cactcatcga cacgctgatc gggcgccttg ccaagggcgg cgagattgcg attgccggct
     3601 tctacgagac tccgatcagc ttcgccttcc cgcccgcctt catgcgggaa gcgcggctgc
     3661 gcatcgcggc cgagtgggcg gctgaagaca ttgccgctgt ccgcagtctt atcgacggcg
     3721 gcaagctctc cttggacggc ctgatcacca accgcgtaac ggcaacggat gccgaggcgg
     3781 cctatcgcac tgcattcacc gaccccacct gcctcaagat ggtcctcgac tggagaacga
     3841 tccaatgaac agcattgacg tcaatctcgt tcgccaggcg aagctccgcg aggaagcggc
     3901 gatcccgccc gatcccgttc acaccggtcc ggtgaagaaa gaaacccaga tcatcgcgat
     3961 ctatggcaag ggcggcatcg gcaagagctt caccctggcc aacctctcct acatgatggc
     4021 gcagcagggc aagcgggtgt tgctcatcgg ttgcgacccc aagagcgaca ccacttcgct
     4081 gctcttcggc ggcaaggcct gccccaccat catcgagacc tcggcgcgca agaagctggc
     4141 tggcgaagag gtcaagatcg gcgacgtctg cttcaagcgc gacggcgtct tcgccatgga
     4201 acttggcgga cccgaagtgg gccgcggctg cggcgggcgc ggcatcatcc atggtttcga
     4261 actcctcgag aagctcggct tccacgaatg gggtttcgac ttcgtcttgc tcgacttcct
     4321 gggcgacgtt gtctgcggcg gcttcggcct gccgatcgcc cgcgacatgt gccagaaggt
     4381 catcatcgtc ggctccaacg acctgcagtc gctctatgtg gccaacaatg tctgttcggc
     4441 ggtcacctac ttccgcaagc tggggggaaa tgtcggcgtg gcgggcattg tcgtgaacaa
     4501 ggacgacaac accggcgagg ctcatgcctt cgcggagaaa gtcggcatcc cggttctcgc
     4561 ctccattccg gccaatgaag acatccggcg caagagcgcc aattacgaga tcatcggcaa
     4621 gcccggcggc gactggggag cgctcttcga agccctcgcg ctcaatgtcg ccgacgcgcc
     4681 gccgcagcat ccatccccgc tggaccagga cggcctcctg ggcctcttct ccggcgatgc
     4741 cgtcggccgc ggcgtggtgc tccagccggc aagcctggaa gacatgtgcg gtggcaccat
     4801 cgtcgaccgg ccctcgctcg aagtcatcta cgacacggt
//
LOCUS       DBNGVR010000005         3710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1967435, whole genome shotgun sequence.
ACCESSION   DBNGVR010000005 DBNGVR010000000
VERSION     DBNGVR010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1967435"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="AC16A0_00090"
     CDS             <1..1005
                     /locus_tag="AC16A0_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HIR3103753.1"
                     /translation="RGGLNLTHSEPLEQLLGRYGEARSRLEPAIRAFPPEALIAWANG
                     LGVETFVGTSGRVFPKAMKASPLLRAWLRRLDSLGVELRTRTEWPGFDGTPTILAFGG
                     ASWPHLGSNASWLPLFEAAGIRVNPFKPANSRFLVNWSPIFRDRFAGTPVKNVALTYA
                     GHRVRGEVMISQEGIEGGAIYALSKGLREQPGHPLMIDLRPDLTEDALAQRLARPRGK
                     DTQSNFLRKAAGLTPVAISLLRETANAMDAKGIKQVAVPILRPAGIARAISSAGGVAL
                     DEVDANFQLKKVAGTYCVGEMLDWEAPTGGYLLQACLSTAVAAARALAAALAPQRAMG
                     "
     gene            1016..1789
                     /locus_tag="AC16A0_00095"
     CDS             1016..1789
                     /locus_tag="AC16A0_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3103754.1"
                     /translation="MDLKLAGTSALVCGASQGLGFAIAEALALEGCRVGLLARNAAKL
                     EAHVADFAQRGLAAVALPADMGNWLSLVAALQRFGTPSILVNNSGGPPPVDVTAVDPN
                     LWRQQFEIMVLNQMRLTEAVLPAMRQAKFGRILSVASTSIVEPIPVLAVSNALRAALA
                     NWMKTLAGAVAKDGVTVNMLLPGSFATARIESMNARDAEQRGVSVESVVAESTAGIPL
                     GRYGDPQEFGAVAAFLASPRASYVTGQMIRIDGGATRST"
     gene            1786..3135
                     /locus_tag="AC16A0_00100"
     CDS             1786..3135
                     /locus_tag="AC16A0_00100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3103755.1"
                     /translation="MSLLADLRAALGDDAVLAGADIGARYRSDRSETGTALPLAVIRP
                     RTTEEVSAALQLCHAAGQPVIAQGGMTGLAGGGNPAGSEIVISLERLSGIEEIDTASA
                     TMTVKAGTPLETCQQAAAEAGLFLALDLGARGSCQIGGNLATNAGGIRVIRYGMAREQ
                     VLGLEAVLADGTVVTSLNKMLKNNAGYDLKQIFIGSEGTLGLITRAVLRLHPPPGEIV
                     TALCALASFDDVVTLLRRAQGSLGGIVAFEAMWCDYFAFNAAALGLSFFADEHGFWTI
                     LESTMEKEAVESFLASCIEDGLVADALIAQSEAQARQIWSVREGYPIEKLPNLLNFDV
                     SLPIARIGAYAEACTAALMATWPAAHVSFYGHVGDSNVHICVSTRYGPGEDMHSVDDI
                     VYGTLRDFGGSISAEHGIGTLKRPYLHVSRSPAELALMRQLKQALDPKGILNPGKVI"
     gene            3135..>3710
                     /locus_tag="AC16A0_00105"
     CDS             3135..>3710
                     /locus_tag="AC16A0_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HIR3103756.1"
                     /translation="MDALIRAVNAPLAFRLVLALPAIIMLSRFALSGGGLGHVLQASG
                     EWAARLLIVTLAVTPIRMIFKGHHWPMWLFKRRRDLGLAAFLYALLHLAIYVVRQSNI
                     HVVLYDLQYKEYFTGWLALAIMLMLTLISNDWLLHSLGLWWKRLQRAVYLAALAVFLH
                     WIWMKLDNTPALAHFVPLVLLEAWRLWYRFAR"
BASE COUNT          554 a         1354 c         1175 g          627 t
ORIGIN      
        1 cgcggcgggc tgaacctcac gcattccgag ccgctggagc aattgctcgg ccgctacggc
       61 gaggcgcgca gccggctcga acctgcaatc cgcgcctttc cgcccgaggc gctgatcgcc
      121 tgggccaatg gcctcggcgt cgaaaccttc gtcggcacgt cgggccgcgt ctttcccaag
      181 gccatgaagg cctcgcccct gctccgcgcc tggctgcgcc gcctggacag cctcggcgtg
      241 gaactcagga cccgcaccga atggccgggc ttcgacggaa cgcccaccat ccttgctttc
      301 ggcggcgcat cctggccgca tctcggctcg aacgcgtcct ggctgccgct gttcgaggcc
      361 gcgggcatcc gcgtcaatcc cttcaagccc gccaacagcc gcttcctcgt caactggtct
      421 cccatcttcc gcgaccgctt cgccggcacg ccggtgaaga acgtggcact cacctatgcc
      481 gggcatcgcg tgcgcggcga ggtgatgatc tcgcaggagg gcattgaggg cggcgccatc
      541 tacgcgctct ccaagggcct gcgcgagcag cccggccatc cgctgatgat cgatctccgc
      601 cccgacctga cggaagacgc gctggcccag cgcctcgccc gcccgcgcgg caaggacacg
      661 cagtcgaact tcctgcgcaa ggccgcgggc ctcacgccgg tcgccatctc gctgctgcgc
      721 gagaccgcca atgccatgga tgcgaagggc atcaagcagg tggccgtccc catcctgcgg
      781 ccggccggga ttgcgcgcgc catctcgtcg gccggcggcg tggcgctgga cgaggtcgac
      841 gcgaatttcc agctgaagaa ggtggcgggc acctattgcg tgggcgaaat gctcgactgg
      901 gaagccccca ccggcggcta tctgctgcag gcctgcttgt cgaccgccgt ggcggcggcg
      961 cgggcactgg cggcggcgct tgcgccgcag cgcgcaatgg gctgaactcg gcaccatgga
     1021 tctcaaactc gccggaacat cggccctggt ctgcggcgca agccagggcc tgggtttcgc
     1081 cattgccgaa gcgctggccc tcgaaggctg ccgcgtcggc ctgctcgctc gcaatgccgc
     1141 gaagctcgag gcgcatgtcg ccgacttcgc acagcgcggc ctcgccgccg tggcgctccc
     1201 cgccgacatg gggaactggc tctccctcgt cgccgcgctg cagcgtttcg gcacgccgtc
     1261 gatcctcgtc aacaattccg gcgggccgcc gccggtcgat gtcaccgccg tcgatcccaa
     1321 tctctggcgc cagcagttcg agatcatggt gctgaaccag atgcgcctca ccgaggccgt
     1381 ccttccggcc atgcgccagg cgaagttcgg ccgcatcctg tcggtcgcct ccacctccat
     1441 cgtcgagccg atcccggtgc tggcggtttc caacgcgctg cgcgccgcgc tggccaactg
     1501 gatgaagacg ctggcgggcg cggtggcgaa agacggcgtc accgtcaaca tgctgctgcc
     1561 cggcagtttt gccacggcgc ggatcgagtc gatgaatgcc cgcgatgccg agcagcgcgg
     1621 cgtgagcgtc gagagcgtcg ttgccgaatc gacggccggc atccccctgg gccgctacgg
     1681 cgatccgcag gaattcggcg ccgtcgccgc cttcctcgcc agcccccggg cctcctacgt
     1741 gacgggccag atgatccgca tcgacggcgg cgccacccgc tcgacatgag cctcctcgcc
     1801 gatctgcgcg cggccctcgg cgacgatgcg gtactcgccg gtgccgacat cggcgcccgc
     1861 taccgctccg accgcagcga gaccggcacc gcgctgccgc tggccgtcat ccgtccacgg
     1921 acgacggagg aggtgtcagc cgccttgcag ctctgccatg ccgcgggcca gcccgtcatc
     1981 gcgcagggcg gcatgacggg cctcgcgggc ggcggcaatc cggccggcag cgagatcgtc
     2041 atcagcctcg aacgcctctc cggcatcgag gagatcgaca cggcctcggc caccatgacg
     2101 gtgaaggccg gaacgccgct cgagacctgc cagcaggccg cagccgaagc cgggcttttc
     2161 cttgcgctcg atctcggcgc gcgcggctca tgccagatcg gcggcaatct cgccaccaat
     2221 gccggcggca tccgcgtcat ccgctacggc atggcgcgcg agcaggtgct gggactcgag
     2281 gccgtgctgg ccgacgggac cgtcgtcacc tcgctcaaca agatgctgaa gaacaatgcg
     2341 ggctacgacc tgaagcagat cttcatcggc tcggaaggca ccctcggcct catcacccgc
     2401 gccgtgcttc ggctccatcc gccgccgggc gagatcgtca ccgcgctctg cgcgctggcc
     2461 agtttcgacg atgtcgtgac gctgctgcgc cgcgcgcagg gtagtctcgg cggcatcgtc
     2521 gccttcgagg ccatgtggtg cgactacttc gccttcaatg ccgcggcact cggcctgagc
     2581 ttcttcgccg atgaacacgg cttctggacc atcctcgaaa gcaccatgga aaaggaagcc
     2641 gtcgagtcct tcctggcttc ctgcatcgaa gacggcctcg tcgccgacgc gctgatcgcc
     2701 cagtccgagg cgcaggcgcg gcagatctgg tcggtgcgcg agggctaccc gatcgagaag
     2761 ctgcccaatc tcctgaactt cgacgtgagc ctgcccatcg cgcgcatcgg cgcatatgcc
     2821 gaggcctgca ccgcagcact catggcgaca tggcccgcgg cgcatgtctc cttctacggc
     2881 catgtgggcg acagcaatgt ccatatctgc gtgtccacgc gctatggccc cggcgaggac
     2941 atgcacagcg tcgacgacat cgtctatgga accctcaggg acttcggcgg ctcgatctcg
     3001 gcggagcacg gcatcggcac gctgaagcgc ccctatctgc atgtctcccg cagcccggcc
     3061 gagctcgccc tgatgcgcca gctcaagcag gctctggatc cgaagggcat cctcaatccc
     3121 ggcaaggtca tctgatggac gcgctgatcc gcgcggtcaa cgcgccgctg gccttccggc
     3181 ttgtactggc gctccccgcc atcatcatgc tctcgcgctt cgccctgtcc ggcggcgggc
     3241 ttggccacgt cctgcaggcc tcgggcgagt gggcggcgcg cctcctcatc gtgacgctgg
     3301 cagtcacacc catccgcatg atcttcaagg ggcaccactg gcccatgtgg ctgttcaagc
     3361 gccgccgcga cctgggcctt gccgccttcc tctacgcttt gcttcatctg gccatctacg
     3421 tggtacggca gtccaacatc cacgttgtgc tctatgatct tcagtacaag gagtatttca
     3481 ccggctggct ggcgctggcg atcatgctga tgctgacgct gatcagcaac gactggctgc
     3541 tgcacagcct cggcctatgg tggaaaagac tgcagcgcgc ggtctatctc gcggctctgg
     3601 ccgtcttcct gcattggatc tggatgaagc tcgacaacac gccggcgctc gcgcatttcg
     3661 tccccctcgt cctgctcgaa gcctggcgcc tgtggtaccg tttcgcccgc
//
LOCUS       DBNGVR010000006         2945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5110729, whole genome shotgun sequence.
ACCESSION   DBNGVR010000006 DBNGVR010000000
VERSION     DBNGVR010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5110729"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="AC16A0_00110"
     CDS             <1..795
                     /locus_tag="AC16A0_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR3103757.1"
                     /translation="VVPTAGVDVRMPFVADHGFAQSVLTPVAQIISTTDEQDADDRSD
                     EDAITLNFDHTNLFLSDRFTGYDRYEGGTRVNLGVVYNLLAADGGFLRASFGESFHIA
                     GENSFVEGSGMNGTSSDLVGGLAYQPNDYLRFTYEARVEEDLSRINVQEAMVSLTLDR
                     ISGSLAYADVAAAEAYGRDDREKQVWGDAVYRLTGAWNVFGGFRYDLEEDTFMEKLIG
                     IGFDCECMNARLTYSEDKDLDDGVDRTLKLSVELRTIGQMDGGFNF"
     gene            870..1793
                     /locus_tag="AC16A0_00115"
     CDS             870..1793
                     /locus_tag="AC16A0_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3103758.1"
                     /translation="MAESQGVVAVVNDQPITERDISQRIALMKILGDSKTDSSRKGAL
                     KSLVDEEVKLLELEKFKLTPTDVEVNKQMERMAKGMDTTTAGLLEKLKKQGIGEKAFR
                     RYVTVLVGFNRLISGKYRNDLEVDPKAVDQKFTEIKREYADYAAKIKNDPRMKPVTVY
                     TMREILLPIDSEDPMLLQARAAEASALGQRIKGCGNIKGAAEGIFDVRVGKTMEVDAA
                     KLPKPLRSALDQAGVGRAIGPMRSKEGIQLLALCSSRKLSPEIPKFEPPTRKQVEAMV
                     VNEKYAKFEDDYMKTARNSVYIEYRDTSYSQ"
     gene            1793..2800
                     /gene="pdxA"
                     /locus_tag="AC16A0_00120"
     CDS             1793..2800
                     /gene="pdxA"
                     /locus_tag="AC16A0_00120"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018241452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3103759.1"
                     /translation="MGAALQPPILLTGGEPAGVAPEITAAAWAALRHDAAATFCLLGD
                     AAYWTARNPRTPFCVIGDAREAAAAFADAVPVLHRPLAAHPEPGVIDPATAPQVIAAI
                     DEAVDLAFAGAVSAIVTNPINKDALYAAGFRHQGHTDYLAHLSARHGNPVNEVMMLIG
                     GDLRAVPVTVHIPLADVPRALTREAILAQARVTARDLRRYFGIARPRLTFTGLNPHAG
                     ENGAMGREEIEVIAPALEQLRAEGLDVTGPVSADTAFHPEARATYDAILCMYHDQALI
                     PVKTIDFHGGINVSLGLPFIRTSPDHGTALGIAGKGLARPDSLIAALRLAAQMARAAR
                     D"
     gene            2793..>2945
                     /locus_tag="AC16A0_00125"
     CDS             2793..>2945
                     /locus_tag="AC16A0_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HIR3103760.1"
                     /translation="MIDDGLPPLREVIARHGLQARKSLGQNFLFDLNLTRRIARAAAP
                     LEGFTIV"
BASE COUNT          567 a          936 c          908 g          534 t
ORIGIN      
        1 gtcgttccga cggcgggcgt cgacgtccgc atgcccttcg ttgccgacca cggatttgcc
       61 cagagcgtct tgacgccggt ggcgcagatc atctcgacga ccgatgagca ggatgccgat
      121 gatcgcagcg acgaagacgc catcaccctg aacttcgatc acaccaacct gttcctctcc
      181 gatcgcttca ctggctatga tcgttacgaa ggcggaaccc gggtcaatct tggcgtggtc
      241 tacaacctgc tggcagccga cggcggcttc ctgcgggcaa gcttcggcga gtccttccac
      301 atcgccgggg agaacagttt cgtggagggg tccgggatga acgggacgtc gtccgatctg
      361 gtcggcggcc tcgcctatca gccaaacgac tatctgcgct tcacctatga ggcccgggtc
      421 gaagaggatc tttcccgcat caatgtccag gaggcgatgg taagcctcac tttggacagg
      481 atttcagggt ctttggccta tgccgacgtt gccgcggccg aagcttacgg gcgtgacgac
      541 cgtgagaaac aggtctgggg cgatgcggtc taccgcctga cgggagcctg gaatgtcttc
      601 ggtggcttcc gttacgatct ggaggaggac accttcatgg aaaaactgat cggaatcggg
      661 ttcgactgtg aatgcatgaa tgcccgcctg acctacagcg aagacaagga cctggatgac
      721 ggggtcgata ggacgctgaa actgtcggtc gaactgcgga ccatcggaca gatggacggc
      781 ggctttaact tctgaaagtg acgatgagac ttttgttgcg aattgctgtc tgtgccgggc
      841 tggccatcgc tgtgccggca ttgcctgcct tggccgagag ccagggtgtc gtggcggtcg
      901 tcaatgacca gccgatcacc gagcgggata tcagccagcg cattgccctt atgaagatcc
      961 ttggagactc caagacggac tcttcccgca agggtgccct caagtccctg gtagacgagg
     1021 aagtgaaact gcttgagctc gagaaattca agctcacccc gacggatgtc gaggtcaaca
     1081 agcagatgga gcgcatggcc aagggcatgg acacgaccac ggccggcctg ctcgaaaagc
     1141 tgaagaaaca gggtatcggc gagaaggcgt tccgtcgcta cgtcacggtg cttgtcggct
     1201 tcaatcgcct catttccgga aagtatcgca atgatctcga ggtcgatcca aaggcggttg
     1261 accagaaatt cacggagatc aagcgcgagt atgccgacta cgcagcgaag atcaagaacg
     1321 acccgcgcat gaagccggtc accgtctaca cgatgcgcga gatcctgctg ccgattgaca
     1381 gtgaagaccc catgctcctg caggcgcgcg cggcggaggc ttccgcactc ggtcagcgca
     1441 tcaagggttg tggcaatatc aagggcgccg ccgagggcat tttcgacgtc agggtgggca
     1501 agacgatgga agtcgatgcc gcgaagctgc cgaaaccttt gaggtcggcg ctggaccagg
     1561 ccggggttgg ccgggcgatc ggcccgatgc ggagcaagga gggcatccag ctgctcgcct
     1621 tgtgcagcag ccgcaagctc agccccgaga ttccgaagtt cgagccgccg acccgcaagc
     1681 aagtcgaggc gatggtcgtg aacgagaaat acgcgaagtt cgaagacgac tacatgaaga
     1741 cggcccggaa ttcggtctac atcgagtatc gcgacaccag ctactcccag tgattggcgc
     1801 cgccttgcaa ccgcccattc tgctgaccgg cggcgaaccg gcaggcgttg ctcccgagat
     1861 taccgccgca gcctgggctg cattgcgcca cgatgccgcc gccaccttct gcctgcttgg
     1921 tgatgccgcc tactggacgg cccgcaatcc gcgcacgccc ttctgcgtca tcggcgatgc
     1981 ccgcgaggcc gccgccgcct tcgccgatgc cgtaccggtg ctgcatcggc cgctcgcggc
     2041 ccacccggag cctggcgtca tcgatccggc aacggcgccg caggtaattg cggccatcga
     2101 cgaggcggtt gacctggcct ttgccggcgc ggtctcggcc atcgtcacca atccgatcaa
     2161 caaggatgcg ctctacgccg ccggatttcg ccaccagggc cataccgact atcttgccca
     2221 tctctcggca cgtcacggga acccggtaaa cgaggtgatg atgctgatcg gcggcgatct
     2281 gcgcgccgtt cccgtcaccg tccacattcc gctcgccgat gtgccgcgcg cgctcacccg
     2341 cgaggccata ctcgcgcagg ctcgcgtcac cgcgcgcgat ctccggcgct acttcggcat
     2401 tgcccgtccg cgactgacct tcaccggact caatccgcat gccggcgaga atggcgccat
     2461 gggccgcgag gagatcgagg tgatcgcacc tgcgcttgag cagctgagag ccgaaggcct
     2521 cgacgttacg ggtccggttt cggccgatac ggcctttcac cccgaggccc gtgctactta
     2581 tgacgccatt ctctgcatgt atcacgacca ggcgctgatc ccggtgaaga ccatcgactt
     2641 ccacggcggc atcaacgtaa gcctcgggct tcccttcatc cgcacctcgc cggaccacgg
     2701 cacggcgctg gggattgcag gcaagggcct tgcaaggcca gacagcctga tcgccgccct
     2761 caggctcgcc gcgcagatgg cgcgtgccgc ccgtgattga cgacggactg ccgccgctgc
     2821 gcgaggtcat cgcgcgccac ggtctgcagg cccgcaagag tcttggacag aacttcctct
     2881 tcgatctcaa tctcacgcgc cgcatcgcga gggccgccgc acctctggaa ggcttcacca
     2941 tagtc
//
LOCUS       DBNGVR010000007         6385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2754395, whole genome shotgun sequence.
ACCESSION   DBNGVR010000007 DBNGVR010000000
VERSION     DBNGVR010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2754395"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16A0_00130"
     CDS             complement(<1..242)
                     /locus_tag="AC16A0_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR3103761.1"
                     /translation="MRPLILALVIGLLALFRPAVAEEQLLSKLEPITIATEDDAKMFT
                     VEIADTDALRARGLMFRQRLPEDRGMLFDFGTPREAA"
     gene            complement(281..862)
                     /locus_tag="AC16A0_00135"
     CDS             complement(281..862)
                     /locus_tag="AC16A0_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3103762.1"
                     /translation="MAASGAHKGGEVAGLPQAPEANVRIIQVNGLVKWFDVARGYGFI
                     IPDNGMQDILLHLSCLKRDGFEAPLEGARVSCEVVERQKGYQCLRVLSVDLSTAIHPV
                     ERKSRAHTTVTPTTGWIRVKVKWFNRARGFGFVSEGEGQPDIFVHMETLRRTAIAELQ
                     PDTWVYVRFGDGPKGKMAAEVRLTLEGGPAHTN"
     gene            complement(1170..1916)
                     /locus_tag="AC16A0_00140"
     CDS             complement(1170..1916)
                     /locus_tag="AC16A0_00140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3103763.1"
                     /translation="MVQRARRAVVFTGAGMSTESGIPDFRSPGGFWTRYKPIEFSAFM
                     SSEEARREAWTRFFAIHESLSAARPGAGHRVISELMRRGHVSHVITQNIDGLHAASGV
                     PPDRIIELHGNGTYAACLGCGKRHELAWARDEIELKGRTPACIGCGGIVKSATISFGQ
                     AMPEQEMADARAATLNADLFLALGSSLQVFPAAGFPVMAKQNRAQLVIVNREATGLDG
                     LADLVVHAELGDVLSALLARLALTSANHLG"
     gene            1961..2380
                     /locus_tag="AC16A0_00145"
     CDS             1961..2380
                     /locus_tag="AC16A0_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3103764.1"
                     /translation="MKYLHTMIRVSNLEQSLDFFINKLGFVEQRRYDNEKGRFTLVFV
                     AAPESPDAPVELTYNWDPEVYTGGRNFGHLALGVDHIYDYCANLQKHGIVINRPPRDG
                     RMAFIRTPDGISIELLQKGEVLPVAEPWASMPNTGSW"
     gene            complement(2385..2762)
                     /locus_tag="AC16A0_00150"
     CDS             complement(2385..2762)
                     /locus_tag="AC16A0_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3103765.1"
                     /translation="MSFQTRRRSHAPDVIAPDGSEVRILGATARGSMAEFTLPPGAVS
                     KPVAHRTVEEVWVFIGGKGRIWRRHANIEDVTDVHPGISITIPVGTAFQFRCDGEEPL
                     VAVGITMPPWPGMDEAYAVDGKW"
     gene            complement(2759..3505)
                     /locus_tag="AC16A0_00155"
     CDS             complement(2759..3505)
                     /locus_tag="AC16A0_00155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3103766.1"
                     /translation="MFDDLKGKVVVVTGGVTGIGGAAALAFAEAGAKVLAQYFGGGPE
                     LAKIEAAGIAAIKLDLTAATAPKALVDAAIARFGRIDVLVNNAGSLVARVPVTELDDE
                     FIDQVFDLNCRQLIHCCRIAAGLMKGQGSGNIINVTSIAARNGSSPGGSVYAGAKAFV
                     SAFSKTLAKELVSHGIRVNCVSPGTIHTAFHDRFSDEAKREAARKTIPMQRLGLAEDC
                     AGTFLYLASDRASGYVTGQVIEVNGGQLMP"
     gene            3560..3946
                     /locus_tag="AC16A0_00160"
     CDS             3560..3946
                     /locus_tag="AC16A0_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103767.1"
                     /translation="MSRGLAVWLFIMLVETLHGLLRGLLLVPRVGEETAGRIGWPIGL
                     VIVLGISIALAPWMAIRDTSALLRLGGLWAVLTLIFELTIGLLRGLDLPRLLAELNPL
                     AGGLTIYSLAAMFLAPLLAARLRGLT"
     gene            3943..4719
                     /locus_tag="AC16A0_00165"
     CDS             3943..4719
                     /locus_tag="AC16A0_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103768.1"
                     /translation="MNDFLKRSWVAFPRESAVLDWIAAAGPAAQRVTSDPGNEHWFRH
                     GRTWFAGVNALPNDSTGAVPDGPAFKGAAIDFLTRHTETAAIAFDRGQVSVCYPGYPK
                     AQHGETDAAVRFRRNRDAAHVDGLIGEGEPKRRFLREPHAFVLGIPLCNVAAETSPLV
                     VWEGSHEIMRSAFLRVLSAHDPKDWPSVDLTDAYTAARRDVFSSCVRVEVCTSLGQCY
                     VLHRLALHGVAPWGRSNAKERPVIYFRPLLDTLEPWLARP"
     gene            complement(4723..5643)
                     /gene="tsf"
                     /locus_tag="AC16A0_00170"
     CDS             complement(4723..5643)
                     /gene="tsf"
                     /locus_tag="AC16A0_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR3103769.1"
                     /translation="MAEITAAMVKDLREKTGAGMMDCKAALSANNGDMEAAIDWLRAK
                     GLAKAAKKSTRVAAEGLVGIAVNGLTGAAVEVNSETDFVARNEKFQAMVAEISKLALA
                     AGGDTEKLKTMKYPGSGHAVAEYVAEMVATIGENMSVRRTAVLSVKDGVIGSYVHNQA
                     APGMGKIGVLVALESTGKKDELAAFGRLLAMHVAATNPVAMTIDGVPAETLNREKAIL
                     EEKNAGKPANVLEKIIANGLKTFSKENCLMDQVYVHDGSKTVTQIIGEVGNKAGAPVK
                     LLGYIRMQLGEGIEKQADDFAAEVAKAAQG"
     gene            complement(5719..6385)
                     /gene="rpsB"
                     /locus_tag="AC16A0_00175"
                     /pseudo
     CDS             complement(5719..6385)
                     /gene="rpsB"
                     /locus_tag="AC16A0_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
BASE COUNT         1118 a         2067 c         1947 g         1253 t
ORIGIN      
        1 gccgcttcgc gcggggtgcc gaagtcgaac agcatgcccc ggtcctcggg aaggcgttgg
       61 cggaacatca ggccgcgggc acgcaaggca tcggtatcgg caatctcgac cgtgaacatc
      121 ttcgcatcgt cttcggtggc gatggtgatg ggttcgagct tggagagtag ctgctcctcc
      181 gccacggcag ggcggaacaa agccagaaga cctatgacaa gagccaggat gagcgggcgc
      241 atagatgact tctctccggc aattgcagcg gaagaagggc tcagttggtg tgtgccggtc
      301 cgccttcgag ggtcagccgc acttcggccg ccatcttgcc tttcgggcca tcgccaaagc
      361 ggacgtagac ccaggtgtca ggctgcaact cggcaatggc cgtgcggcgc aaggtttcca
      421 tgtgaacgaa gatatcgggc tggccttcgc cctcggacac gaaaccgaaa ccacgggcgc
      481 ggttgaacca cttcaccttc acgcgaatcc agccggtcgt cggcgtgacc gtggtatggg
      541 cccgactctt gcgttcgacg ggatgaatgg ccgtggacag gtcgaccgac agcacgcgga
      601 ggcactgata gcccttctgc cgctccacca cctcacagga cacgcgcgcg ccctccagcg
      661 gtgcctcgaa cccatcccgc ttcagacagc tcaggtgaag caggatatcc tgcatgccgt
      721 tgtcggggat gatgaaccca tagccccgtg ccacatcgaa ccattttacc aggccattca
      781 cctggatgat tcgaacattc gcctccggag cttgcggaag acccgcaact tccccgccct
      841 tatgggcgcc actcgccgcc atgacttacc ccaatgtccc actggcggaa cgcgtgttgc
      901 ctctgcaccg ccagtcaatg gttaatctgc gcaaaaccca aggctttgta cagggcattg
      961 cgtcgtctgc cggtgtggat aagcgctgct catgcaacaa ggcttcgtcc gcgccagtgg
     1021 cttagcaatt caagctatgg gaatcacgcg gcgatttggc ctgacgggag tttccaccaa
     1081 ctgaattgct ttgttgcaag catgcaacat cgccaatctg tttttgctgc aacccgattc
     1141 acgaccagcg gcgctgatcg caggctctcc taccccaagt gattcgccga ggtcagcgca
     1201 agccgcgcga gcagtgccga cagcacatcg cccagttcgg cgtgaaccac caggtcggca
     1261 aggccgtcga gtcccgtcgc ctcgcgattg acgatcacca gctgcgcccg gttctgcttg
     1321 gccatgaccg gaaagccggc cgccggaaac acctggagcg aagagcccag cgccagaaag
     1381 agatcggcgt tcagggtcgc ggcgcgagcg tcagccatct cctgctccgg catggcctgc
     1441 ccgaaggaga tggtagccga cttcacgatg ccgccacagc cgatgcaggc gggcgtccgc
     1501 cccttcagct cgatctcgtc cctggcccag gccagctcgt ggcgctttcc gcaaccgagg
     1561 caggccgcat aggtgccgtt gccgtgcagc tcgatgatcc ggtcgggagg aaccccggat
     1621 gccgcatgaa gcccgtcgat gttctgggtg atgacgtgcg agacgtggcc gcgccgcatc
     1681 agctcggata tcacgcgatg accagcgccg ggccgcgccg ccgacaggct ttcgtggatg
     1741 gcgaaaaaac gcgtccaggc ttcccggcga gcctcttcgg atgacatgaa ggcgctgaac
     1801 tcgatgggct tgtagcgggt ccagaagccg ccgggcgacc ggaagtccgg aatgccgctc
     1861 tccgtggaca tgccggcgcc ggtgaagacc accgcgcgcc ttgcgcgctg gaccattgca
     1921 gccagttctt ctatgtctcg gtcaatcacg aggagatccc atgaagtacc tgcatacgat
     1981 gatccgcgtc tccaatcttg agcagtcgct cgacttcttc atcaacaaac tcggctttgt
     2041 cgagcagcgg cgctacgaca acgaaaaggg ccgcttcacc ctcgtcttcg tggccgcgcc
     2101 cgaaagcccc gacgcaccgg tggaactgac ctacaactgg gaccctgaag tctatacggg
     2161 cgggcgcaac ttcggtcacc tcgcactcgg cgtcgaccac atctacgact actgcgccaa
     2221 cctgcagaag cacggcatcg tcatcaaccg gccgccgcgc gacggccgca tggccttcat
     2281 ccgcacgcca gacggaatct ccatcgagtt gctgcagaag ggcgaggtgc tgccagttgc
     2341 cgaaccctgg gccagcatgc ccaataccgg cagctggtag agcgttacca cttgccatcg
     2401 acagcatagg cttcgtccat tccaggccag ggcggcatgg tgatgcccac cgcgacgagc
     2461 ggctcttccc catcgcagcg gaactggaag gccgttccta ccggtatcgt gatggagatg
     2521 ccggggtgca catcggtgac atcttcgatg ttggcatggc ggcgccagat ccggcccttt
     2581 ccgccaatga agacccacac ttcctccacc gtgcgatggg caaccggctt cgacacagca
     2641 cctgggggaa gcgtgaactc tgccatgctg ccgcgcgccg ttgcaccaag aatgcggact
     2701 tcggagccat ctggtgcaat gacgtcgggc gcatggctgc gccttcgggt ctgaaaactc
     2761 atggcatcaa ctgcccgcca ttcacctcga tcacctggcc cgtgacatag ccactcgccc
     2821 tgtcgctggc gagatagagg aaggtcccgg cgcagtcctc ggcgaggccg aggcgctgca
     2881 tcggaatggt tttgcgcgcc gcctcgcgct tggcctcgtc tgagaaccgg tcgtgaaagg
     2941 ccgtatggat ggtgcccgga gagacgcagt ttacccggat cccatggctc acaagctcct
     3001 tggccagggt cttggagaaa gccgagacaa aggccttggc cccggcatag acactgccgc
     3061 cggggctgga gccgttgcgg gcggcaatgg aggtgacatt gatgatgttg ccggacccct
     3121 gccccttcat caggccggcg gcgatccggc agcagtgaat gagctggcgg caattgaggt
     3181 cgaagacctg gtcgatgaac tcatcgtcga gttcggtgac gggtacgcgg gccaccagac
     3241 tgccggcatt gttgaccagg acatcgatcc ggccgaaacg ggcgatggcc gcatcgacga
     3301 gcgccttggg cgccgtggcg gcggtaagat cgagcttgat cgctgcaatt ccggcagcct
     3361 cgatcttcgc cagttccggc ccgccgccga agtactgggc cagcaccttc gcccctgctt
     3421 ccgcgaaggc gagcgcggcg gcacctccga tccccgtgac gccgccggtt acaacaacca
     3481 ccttgccttt cagatcgtca aacatagagt ccccttggat tgaaagggcc atcatgcccc
     3541 aagaggaaag gttgcgccat tgtcccgggg cctggcagtc tggctgttca tcatgcttgt
     3601 cgaaacgctg catggcctgt tgcgcgggct tttgcttgtc ccgcgcgtcg gtgaggaaac
     3661 ggctggccgc atcgggtggc cgatcgggct ggtgatcgtc ctgggcattt ccatcgccct
     3721 tgcgccgtgg atggcaatcc gcgacacatc tgcccttctc cgcctcggcg ggctgtgggc
     3781 agtgctgacg ctcatcttcg aattgaccat cgggcttctg cgcggcctcg acttgccgcg
     3841 acttctggcg gaactcaatc cgcttgcggg tggccttacg atctacagcc tcgccgccat
     3901 gttccttgct cccctgctgg cggcccgcct gcgtggcctg acgtgaacga ctttctcaag
     3961 cgcagctggg tggcatttcc gcgggaatcc gctgtcctgg actggatcgc tgcggccggg
     4021 ccggcggcgc agcgtgtgac gtctgatccg ggcaacgaac actggttccg tcatggccgc
     4081 acgtggttcg ccggggtgaa tgccttgccc aacgacagta caggcgccgt ccccgatggt
     4141 cctgccttca aaggcgctgc aatcgacttt ctaacccgcc acacggagac cgcggccatc
     4201 gccttcgaca gggggcaggt ctctgtttgc tatccgggct acccaaaggc ccaacacggt
     4261 gagaccgatg ccgccgtccg cttccgccgc aaccgggatg ccgcccatgt cgacggcctg
     4321 atcggcgaag gggaacccaa acgccgcttc ctgcgggaac cgcatgcctt cgtgctgggg
     4381 ataccccttt gcaacgtcgc tgcggagacg tcgccactcg tggtctggga gggctcgcac
     4441 gagatcatgc gttccgcgtt cctccgtgta ctgagcgctc acgatccgaa ggactggccg
     4501 tccgtcgatc ttaccgatgc ctatacggcg gcgcggcgtg atgtgttttc ctcatgtgtg
     4561 agggtcgagg tctgcacctc tcttggacaa tgctatgtgt tgcaccggct tgccctgcat
     4621 ggcgtcgcgc cttggggccg ttcgaatgca aaggagcggc cggtgatcta tttccggccg
     4681 ctcctcgaca ctctcgaacc ctggcttgct cggccctgac ggtcagccct gcgccgcctt
     4741 ggccacttcg gcggcaaagt cgtcagcctg cttctcgatg ccctcgccca gctgcatgcg
     4801 gatgtaaccc aggagcttta ccggggcgcc ggccttgttg ccgacctcgc cgatgatctg
     4861 cgtcaccgtc ttgctgccgt catggacata gacctggtcc atcaggcagt tctccttgga
     4921 aaaggtcttg aggccgttgg cgatgatctt ttccagcacg ttggcgggct tgccggcgtt
     4981 cttctcctcg agaatggcct tctcacggtt gagtgtttca gccggaacgc catcgatggt
     5041 catggccacc gggttggtgg ctgcaacatg catcgccagc aggcggccga aggctgccag
     5101 ttcgtccttc ttgccggtcg actccagcgc cacgagaacg ccgatcttgc ccatgccagg
     5161 cgccgcctgg ttgtgcacat aggagccgat cacgccgtcc tttactgaca gcacggcggt
     5221 ccggcgcacg ctcatgttct cgccgatcgt ggcgaccatc tcggctacgt actcggcaac
     5281 cgcgtgcccg gagcccggat acttcatggt cttgagcttc tcggtgtcac cacctgcggc
     5341 cagcgccagc ttcgagatct cggccaccat cgcctggaac ttctcgttcc gggcgacaaa
     5401 gtcggtctcg gaattcacct cgacggcagc gccggtgaga ccgttgacgg caattccgac
     5461 caggccttca gccgcgaccc gggtggactt cttggccgcc ttggcaaggc ccttggcgcg
     5521 cagccagtcg atggcggcct ccatgtcgcc attgttggcc gacagcgcgg ccttgcaatc
     5581 catcatgccc gcgccggttt tctcgcgcag gtccttcacc atagctgcag tgatctcggc
     5641 catgattgtc ctcttgatct gaaatgggtt cgaggcccgc gatcctgcgc gggcccctgg
     5701 ttcgatcctc ggcgatcagg ccgggatctc ttcggtgatg gggttctcgg cagcgccgac
     5761 atccacgccc tgaccggtct gggccatgga gattccttcg atgcaggcgc ggctgatcag
     5821 ctcgcaatag agtgcaacgg cacggccggc atcatcattt cccgggatcg gataggtgac
     5881 gcccgtggga tcggaattgg tgtcgatcac cgcgatcacc ggaatgccga gacgattggc
     5941 ttccttgatc gcgagctgct ccttgttggt atcgatcacg aacagcaggt caggtgttcc
     6001 gcccatgtcc ttgatgccac caagcgcctt gtcgagcttt tccttctcgc gggccagggt
     6061 caggacttca cgcttggtga gacccgagcc ttccttggag atcatgtcgt cgatgttgcg
     6121 gagatgcttg atcgagttcg agattgtctt ccagttggtg agcatgccgc ccagccagcg
     6181 ggaattcaca taatactggg cgctgcgctt cgccgactcc gcgatctgct ctgaggccga
     6241 acgcttggtt ccgacgaaaa gcacgcggcc gcccttggca accgtctcag tgaccgtcag
     6301 cagcgcctgg tgcaacagcg gaacggtctg cgacagatcg atgatgtgga tgccgttacg
     6361 gctgccgtag atgaaggact tcatc
//
LOCUS       DBNGVR010000008         2199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4522705, whole genome shotgun sequence.
ACCESSION   DBNGVR010000008 DBNGVR010000000
VERSION     DBNGVR010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4522705"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..163
                     /locus_tag="AC16A0_00180"
     CDS             2..163
                     /locus_tag="AC16A0_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIR3103770.1"
                     /translation="MAVGAVLVVLLAGLWNMFRGGSSNTSQTLMRWRVILQFVAIVIL
                     MASLWFAQR"
     gene            232..579
                     /locus_tag="AC16A0_00185"
     CDS             232..579
                     /locus_tag="AC16A0_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR3103771.1"
                     /translation="MVLTQKEAGVRRDFFPKMKRVLANVPFAEDVVAAYFCAFDPATP
                     MRAKTILIGALAYFILPLDVVPDFVLGLGFTDDMAVLLAAVNVVRVHLHQRHRDKARD
                     TLDRMRQGDPAAV"
     gene            671..1360
                     /locus_tag="AC16A0_00190"
     CDS             671..1360
                     /locus_tag="AC16A0_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR3103772.1"
                     /translation="MFDPKKLLEQFLGGNAPGTGQKGLSPDLIKGLAGGAAAGGLAGI
                     LLGGKSTKKLAKTALKVGGTAALAGLAYKAYNDWQASKQPSAPEAPNMKDITPRPEGT
                     PFLPRIERERDTQSLALLTAMIAAAKADGHIDAQEQQKIFGKLDEMDLDTESKAFIMD
                     ELRKPLNIDQVVAAATTPELAVEIYAASVLAIDPDDPAEQAYLAMLASRLKLEPGLRA
                     AIERETAKVLA"
     gene            1370..>2199
                     /locus_tag="AC16A0_00195"
     CDS             1370..>2199
                     /locus_tag="AC16A0_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3103773.1"
                     /translation="MGYPEPMDDQGGSAVPTLSSLARESFYRLFADEAIPLAGNIAFR
                     SLFSVFPFLIFLTALGGFFGNEALAQSIVDYLLSVAPEELVKPLAGEIRSILTVPRTG
                     LLSLSALLTIWSAMAGVDSVRVGLNRAYDLREDRSIWVLYLQNVLFVIGSAVLLLTVA
                     LLLVFAPVGIAIINAHAPELQASFSTLEQVRLPIAILLLTAGLLICHRVLPAKQLRVL
                     EVLPGVILTVIVWIALSSAFSYYLVNFNTFASTYASLSGLFAMMFFLYLAALVLILGG
                     "
BASE COUNT          348 a          742 c          672 g          437 t
ORIGIN      
        1 catcgcggtc ggcgccgtgc tcgtcgttct cctcgcaggc ctgtggaaca tgttccgcgg
       61 cgggtcttcc aacacgtccc agaccctgat gcgctggcgc gtcatcctgc agttcgtcgc
      121 catcgtcatc ctcatggcca gtctgtggtt tgcccagcgc tgagggcttg aaaccggtgc
      181 cgcgcgcatc catataacgc agcgtttggg cttgcgcggg aggaggccgg aatggttctg
      241 acgcagaagg aagccggggt ccggcgcgat ttctttccga agatgaagcg ggtgctggcc
      301 aatgtgccct tcgccgagga cgtggtcgcg gcctatttct gcgccttcga tccggccacg
      361 ccgatgcgcg ccaagaccat cctgatcggt gcgctcgcct atttcatcct gccgctggat
      421 gtggtgcccg acttcgtgct gggtctcggc tttaccgacg acatggcggt gctgctggcg
      481 gcggtaaacg tggtgcgggt gcatcttcac cagcgccatc gcgacaaggc tcgcgatacg
      541 ctggaccgca tgcggcaggg agatccggca gcggtttgag aggccgacgt cttgcaaggc
      601 tgcacggtga tgcccacatg tcgcgtcggc attcgcaggc cgggtgcggc cggattttga
      661 aaggttcagc atgttcgatc ccaagaagct tctggagcaa ttcctcggcg gcaatgctcc
      721 cggaaccggc cagaagggcc tgtcacccga cctgatcaag ggccttgcgg gcggcgccgc
      781 cgcgggcggg cttgccggca ttctcctggg cggcaagtcg accaagaaac tggccaagac
      841 cgcgctgaag gtgggcggta ccgccgcgct ggcgggcctt gcctacaagg cctacaacga
      901 ctggcaggcc tcgaagcaac cctccgcacc cgaagcgccc aacatgaagg acatcacgcc
      961 gcggcccgag ggaacgcctt ttctgccgcg catcgaacgc gagcgcgaca cccagagcct
     1021 ggcgctcctc accgcgatga tcgccgcggc caaggccgat ggccacatcg acgctcagga
     1081 gcagcagaag atcttcggca agctcgacga gatggacctc gacaccgagt cgaaggcctt
     1141 catcatggac gagttgcgca aacccctgaa catcgaccag gtggtggccg ccgccaccac
     1201 gccggagctg gcggtcgaga tctatgccgc ttcggttctg gccatcgatc ccgacgatcc
     1261 ggccgaacag gcctatctcg ccatgctggc ctctcggctg aagctcgaac ccggccttcg
     1321 ggccgccatc gagagagaaa ccgcgaaggt cttggcgtaa cgcgcccccg tgggctatcc
     1381 agagcccatg gacgatcagg gcggctctgc ggttccaaca ctttcttcac tggcgcgcga
     1441 gagcttctac cggctcttcg ccgacgaggc gattccgctt gccggcaaca ttgccttccg
     1501 ctcgctcttc tcggtgtttc cctttctcat cttcctcacc gcactcggcg gcttcttcgg
     1561 taacgaagcg ctggcccaga gcatcgtcga ctatctgctc agcgttgccc cggaagaact
     1621 ggtgaagccg ctcgccggcg agatccgctc catcctcacc gtgccgcgga ccggcctgct
     1681 cagcctctcg gcgctgctca ccatctggag cgccatggcc ggcgtcgaca gcgtgcgcgt
     1741 cgggctcaac cgtgcctatg atcttcgcga ggaccgcagc atctgggtgc tctatctgca
     1801 gaacgtgctg tttgtcatcg gctcggccgt gctgctgctg accgtggccc tgctgttggt
     1861 tttcgcgccg gtcggcatcg ccatcatcaa tgcccatgca ccggaactgc aggccagttt
     1921 ctcgacactg gaacaggttc gcctgccgat cgcgatcctt cttctcaccg ccgggctgct
     1981 gatctgccat cgcgtgctgc cggccaagca gctgcgcgtg ctggaggtgc ttcccggcgt
     2041 gatcctcacc gtgatcgtct ggatcgcgct gtccagcgcc ttctcgtact acctcgtgaa
     2101 cttcaacacc ttcgcctcga cctacgcatc gctgtcgggg ctctttgcca tgatgttctt
     2161 tctctacctt gcggcgctgg tgctcatcct cggcggcga
//
LOCUS       DBNGVR010000009         3832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3540666, whole genome shotgun sequence.
ACCESSION   DBNGVR010000009 DBNGVR010000000
VERSION     DBNGVR010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3540666"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC16A0_00200"
     CDS             <1..201
                     /locus_tag="AC16A0_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR3103774.1"
                     /translation="QTDLVAQATVDDAGAADLIKRYPKTLARDFDTNPGAITEADALI
                     AYLQMLGTLVDFKTYDRKANIR"
     gene            214..366
                     /locus_tag="AC16A0_00205"
     CDS             214..366
                     /locus_tag="AC16A0_00205"
                     /inference="COORDINATES: protein motif:HMM:NF017366.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HIR3103775.1"
                     /translation="MSEYESWASFAQTWGTVSFVAFFVLVLLYALNPRNRDQFNQAAR
                     TPLEKD"
     gene            371..1240
                     /gene="ccoP"
                     /locus_tag="AC16A0_00210"
     CDS             371..1240
                     /gene="ccoP"
                     /locus_tag="AC16A0_00210"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR3103776.1"
                     /translation="MSVEKIDNVTGTATTGHEWDGIRELNTPLPRWWLWLFYICIVWG
                     IGYLFLYPAIPLASSYTRGLLGYASRDTIETDMAALKTQRGAVVGQIETASLADIEKS
                     PQMLTVARALGKAAFGTNCGPCHGQGGQGAATYPNLNDDQWIWGGTLDQIHQTIQHGI
                     RNESADSRQGPMPAFGKDGIIPKADIPVIAAYVVTLSGRKPEGTFDLAKGKALFAENC
                     AACHGEDGKGNQEVGAPNLTSNIWLYGGDQKTIEATITNGRGGVMPSWAGRLDPATIK
                     SLAVYVHTLGGGQ"
     gene            1352..2824
                     /gene="ccoG"
                     /locus_tag="AC16A0_00215"
     CDS             1352..2824
                     /gene="ccoG"
                     /locus_tag="AC16A0_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HIR3103777.1"
                     /translation="MTTADSAQPVPVSTETYLDDSPLYEARKDIFPQSVKGRFRTIKW
                     AILIVTLGIYYFLPFVRWDRGVNAPDQAVLIDMVNSRFYFFFIEIWPQELYYVTGLLV
                     LAAIALFLMNAVAGRVWCGYLCPQTVWTDLFYAVERLIEGDRREQIKKKDAGVWTLER
                     VSEKVLKHTLWLLIAWWTGGAWVLYFSDAPTLVKQLVTFQGPVVAYVWIGILTFTTYA
                     LAGWMREQVCLYMCPWPRIQAALTDEHALNVTYRYDRGEPRMSAKKAEAARAKAEPAG
                     DCVDCFQCVNVCPTGVDIREGAQLGCIQCGLCIDACDNVMAKIGRPSGLIGYDTDENV
                     QRRIAREEPVVRIVRPRTILYAVIMVLVGVLMAYTLATRSTLGVSALHDRNPVFVRLA
                     DGSLRNAYTIRLTNKTLDARPFRLEVLGLSDVTLDVIGEPHTAEQSALLEVQPDQTRE
                     LRVLLTVPKGDQPASRPIRFRLTDVVSGATAHVDDFFKGP"
     gene            2840..3364
                     /locus_tag="AC16A0_00220"
     CDS             2840..3364
                     /locus_tag="AC16A0_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR3103778.1"
                     /translation="MALALEPKGFTPMDSGFRLKGWHVLVSLLAFFGVVFAVNFTMMG
                     LAFSTFSGVERASPYQDGLKYNNEIEAARRQDAQGWKVDARLLRDADGAARMTVDARD
                     AEGGVISGLDGTVVLERPADRRQDLRGTLAATAPGRYAASIENVALGQWDVVVEFKRG
                     GERVFMSRSRMILR"
     gene            3389..>3832
                     /locus_tag="AC16A0_00225"
     CDS             3389..>3832
                     /locus_tag="AC16A0_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR3103779.1"
                     /translation="MSPVIDYSALVETRDDGSHRLELAVEGITCAACIGDIERGLASL
                     PGLDRARLNYTNRRLAAEWRAEDFAPAAIVSRLVELGYKAHPFEASREEAREEAEMRC
                     LLRCLAIAGFAAMNIMLFSVSVWSGNASDITGETRDFFHWISALIA"
BASE COUNT          699 a         1222 c         1200 g          711 t
ORIGIN      
        1 cagaccgatc tcgtcgctca ggcaactgtc gacgatgccg gcgcggcgga cctgatcaag
       61 cgctatccca agaccctggc gcgcgacttc gacaccaatc ctggtgcgat caccgaggcc
      121 gatgctctca tcgcctacct gcagatgctc ggcacgttgg tcgacttcaa gacctacgac
      181 cgcaaggcga acattcgttg aggaggctgg atcatgtctg aatatgaatc atgggctagc
      241 ttcgcccaga cgtggggcac cgtcagcttc gtcgccttct tcgtcctcgt gctgctctac
      301 gccctgaatc ccaggaaccg cgaccagttc aatcaggccg cccgcacccc gcttgagaag
      361 gactgaaacg atgtccgttg agaagataga caacgtcacc ggcacggcta ccacggggca
      421 cgagtgggac ggcatccgcg agctgaacac accgctgccg cgctggtggc tgtggctgtt
      481 ctacatctgc atcgtctggg gcatcggata tctgttcctg tacccggcga tcccgctggc
      541 ctccagctac accaggggcc tcctcggcta cgccagccgc gacaccatcg aaaccgacat
      601 ggcagcgctc aagacgcaac gcggcgcggt tgtgggccag atcgaaaccg cctcgctggc
      661 ggatatcgag aagagccccc agatgctcac cgtcgctcgt gcgcttggaa aggccgcctt
      721 cggcaccaat tgcggtcctt gccatggcca ggggggccag ggcgccgcga cctacccgaa
      781 cctgaatgac gaccagtgga tctggggcgg cacgctggac cagatccatc agaccatcca
      841 gcacggcatc cgcaacgaga gcgcggattc acgccagggt cccatgccgg ccttcggcaa
      901 ggacggcatc atcccgaaag cggacatccc cgtcatcgcg gcctatgtcg tgacgctctc
      961 cggccgcaag ccggaaggga ccttcgatct cgccaagggc aaggcgctgt tcgcggagaa
     1021 ttgcgccgca tgccacggcg aagatggcaa ggggaaccag gaagtcggcg ccccgaacct
     1081 gacgtcgaac atctggctct atggcggtga ccagaagacc attgaggcta ccatcaccaa
     1141 cggccgtggc ggcgtcatgc cgtcctgggc ggggcgcctc gatcctgcaa caatcaagtc
     1201 gctcgcggtt tacgtgcaca cgctgggcgg cggtcagtaa agctaagctg ccggagcgga
     1261 ccttcgggtt ccgttccggc ggcggttccg gacccggcgg cgcgaagggc gtcgggtcga
     1321 aatcccccat cgccccttcg aggcattgag catgacgacc gccgacagcg cacagccagt
     1381 gccggtctcg accgagacct atctggacga cagcccactt tacgaggcgc gcaaggatat
     1441 cttcccgcaa tcggtgaagg gtcgcttccg tacgatcaaa tgggcgatcc tcatcgtcac
     1501 gctgggcatc tattatttcc tgcctttcgt gcgctgggac cgcggcgtga acgcccccga
     1561 tcaggccgta ttgatcgaca tggtcaatag ccgcttctat tttttcttca tcgagatctg
     1621 gccgcaagaa ctctattacg tcaccggcct gctggtcctc gcggcgatcg cgctgttcct
     1681 catgaacgca gtcgccggcc gcgtctggtg cggctatctc tgcccgcaga cagtgtggac
     1741 cgacctgttc tatgccgtcg aacggctgat cgagggagac aggcgcgagc agatcaagaa
     1801 gaaggacgcc ggcgtatgga cgctggaacg cgtctcggaa aaggtgctta agcacacgct
     1861 ctggctgctc atcgcatggt ggaccggcgg cgcctgggtg ctctacttct ctgacgcgcc
     1921 gacgctggtg aagcagctcg ttaccttcca ggggccggtc gtcgcctatg tctggatcgg
     1981 cattctcacc ttcacgacct acgcgctggc cggatggatg cgcgagcagg tctgcctcta
     2041 catgtgccca tggccgagga tccaggcggc gcttacggat gagcacgcgc tcaatgtcac
     2101 gtaccgctac gaccgcggcg aaccgcgcat gtcggccaag aaggccgaag cggcgcgcgc
     2161 aaaggcggag ccggccggcg attgcgtgga ctgctttcag tgcgtgaacg tgtgtccgac
     2221 cggcgtcgat atccgggaag gtgcgcagct cggctgcatc cagtgcggcc tgtgcatcga
     2281 cgcctgtgac aacgtcatgg ccaagatcgg ccggcccagc gggttgatcg gctacgatac
     2341 cgatgagaac gtccagcgcc gcattgctcg cgaggagccg gtggtgcgga ttgtccgtcc
     2401 gcgaaccatc ctctacgcag tgatcatggt gctggtaggc gtactgatgg cctacacgct
     2461 tgcaactcgc tcgacccttg gcgtcagcgc ccttcacgac cgcaatcccg tcttcgtgcg
     2521 tctggctgac ggttcgttgc ggaatgccta cacgatccgc ctgacgaaca agacgctgga
     2581 cgcccgcccg ttccgcctcg aagtgctcgg cctgagcgat gtgacgctgg acgtgatcgg
     2641 cgagcctcat accgccgagc agtctgcact gcttgaggtg caaccggatc agacccgcga
     2701 gctccgcgtg ctgctgaccg taccgaaggg cgatcagccg gcatcccgcc cgatcaggtt
     2761 cagactgacc gacgtggtca gtggagcgac cgcccatgta gacgacttct tcaagggtcc
     2821 ttgaacaatg gaaaagacca tggcgctggc acttgagccg aagggtttta caccgatgga
     2881 cagcggcttc cgcctgaaag gctggcatgt actggtgagc ctcctcgcct tctttggcgt
     2941 ggtcttcgcc gtcaatttta cgatgatggg gctggctttt tccacgttca gcggggtcga
     3001 gcgcgcctct ccctatcagg acggactgaa gtacaataac gagatcgagg ccgcccggcg
     3061 ccaggacgcg caaggctgga aggtcgatgc acgattgctc cgcgatgccg acggcgccgc
     3121 ccgcatgacg gtggatgcgc gggacgcgga gggcggggtg atatcggggc ttgacggaac
     3181 ggttgttctg gagcgcccgg ctgatcgtcg ccaggacctg cgcggcactc tcgccgcgac
     3241 tgccccgggc cgctatgccg cgtcgatcga gaacgttgca ctcgggcaat gggacgtcgt
     3301 cgtcgagttc aagcgtggcg gcgagcgggt cttcatgtcg cgaagcagaa tgatcctccg
     3361 ctaaatccgg gtacagagga acctgtcgat gtcccccgtg atcgactact ccgcacttgt
     3421 cgaaacccgc gacgacggtt cccaccgtct tgaattggcg gttgaaggca ttacgtgcgc
     3481 cgcctgcatc ggagacatcg aacgcggcct ggcgtcgctg ccgggtctcg atcgcgctcg
     3541 gctgaactat acgaatcgtc gtctcgccgc agagtggcgc gcggaggatt tcgcacccgc
     3601 cgccattgtc tcccgtctcg tcgagctcgg ctacaaggcc caccctttcg aagcgtctcg
     3661 ggaagaggcg cgggaggagg ccgagatgcg ttgtctgttg cgctgcctcg cgatcgccgg
     3721 cttcgcggcc atgaacatca tgctgttttc cgtgtcggta tggtccggca atgcaagcga
     3781 catcaccggc gagacacgtg acttcttcca ctggatctcg gcgctgatcg cg
//
LOCUS       DBNGVR010000010         5888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1576200, whole genome shotgun sequence.
ACCESSION   DBNGVR010000010 DBNGVR010000000
VERSION     DBNGVR010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1576200"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="AC16A0_00230"
     CDS             complement(<1..1289)
                     /locus_tag="AC16A0_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3103780.1"
                     /translation="MLASSLRAFMMAALAATAFTAQPAAAYEQLDDRLLSGNYLAGRS
                     ASVARDNDLASDYFVRALKADPGNPVLIERVFLLKTAEGDIADSEEYAIQVLGFNSQQ
                     RMARIVLGLRDFRNRRYAEARKNLEVASYTPVGELTSGLLVGWTHAGEGELNLALKAL
                     DRLDRNEAFVNFKNFHAALISDFLGNAIRAEVFYKKAYEQAGTSLRIVQGYGNFLERS
                     GRADEAIRIYATYLEGDDNPLIQASLDGAKAGKKPAPFIPTPGAGAAEALFSLATSMT
                     DEQSIDVALLYTQLALSFNADRAVTLTLLGDILGDMKLDEKAIAAYSEIPASSPLRTN
                     AEMEIGVSLHRLEKKDEAQAKLKDVIAREPDNYDAMVTLGNLYRSNEDYANAAKVYDQ
                     AIALLTEETTTPGHWRIYYFNGIAHERLKQWDIAERN"
     gene            complement(1417..3072)
                     /locus_tag="AC16A0_00235"
     CDS             complement(1417..3072)
                     /locus_tag="AC16A0_00235"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR3103781.1"
                     /translation="MSVEQIQREAMEYDVVIVGAGPAGLAAAIRLKQLSPEISVCLLE
                     KGSEVGAHILSGAVIDPIALNELIPNWKELGAPLNTPVTEDRFYMLGPSGQLRLPNFM
                     MPPLMDNHGNYAVSLGNVCRWLAQQAEGLGVEIYPGFACSELLYREDGSVRGVVAGVM
                     GLGKDGQPKADFQPGMELLGKYVFIAEGVRGSLAKEIIAKYDLAYGREPQKYGLGMKE
                     LWEVRPEVHKQGQVTHTMGWPLGMKAGGGSFMYHLEDNLVSIGFVVHLNYKNPHLFPY
                     MEFQRFKHHPLIRPVLEGGRRIAYGARAINEGGFQSVPKLYFPGGALIGCSAGFVNVP
                     RIKGSHNAMKTGMLAAEAAFAALAAGRSGDELAAYEEAYRNSWVHTDLKRVKNVKPMW
                     TKLGLLGGLALGGLDMWMNQLLGFGLGTWKHGKRDSETLVPAAEAKPIDYPKPDGVIS
                     FDRLTNIAFSATNHEEDQMVHLRLKNPDVPIAVNLPTWDEPAQRYCPAGVYEVVREEG
                     KAPRFQINHQNCVHCKTCDIKDPSQNITWVVPQGGEGPNYPNM"
     gene            3167..3943
                     /locus_tag="AC16A0_00240"
     CDS             3167..3943
                     /locus_tag="AC16A0_00240"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3103782.1"
                     /translation="MTPATAAAALAWLAEMGADEIIGDTPVNRMVAAPAPAAATVTAA
                     RPAAAVVQAARKGEAPADPASCSTLAVIEASLSAFDACPLKKTATNLCYADGNPSARV
                     MLVGEAPGRDEDIQGKPFVGRSGQLLDRMLAAIGLDRKADDRESAVFITNTIFWRPPG
                     NRTPTEAETAMCLPFLLRTIEIQKPDVIVCLGATPAHRLTGRSDGILKLRGKWTEATV
                     SGRKIPLLPTLHPAYLLRQPAQKRLAWRDLLSLRQALDAH"
     gene            3933..4562
                     /locus_tag="AC16A0_00245"
     CDS             3933..4562
                     /locus_tag="AC16A0_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3103783.1"
                     /translation="MPIEWPDLLLFLSASLMLNLTPGNDMMFVLGQSLRGGTRSGIAA
                     SLGIATGSLVHLALVAMGLAVVLARNPAVFEAIRYAGAAYLVYIAIQTLRSGPAILKP
                     APGDASAFKAWRDGAVVNILNPKIIVFFFAFLPPFVKPENGQPLLQLVIFGLLFNIGG
                     TLINFAVAAFAARLAGVFSGNVKIARAFAVLSSALFLVLAARMLFDRKA"
     gene            4650..5189
                     /gene="moaB"
                     /locus_tag="AC16A0_00250"
     CDS             4650..5189
                     /gene="moaB"
                     /locus_tag="AC16A0_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HIR3103784.1"
                     /translation="MSEARSFIPVRIAILTISDTRTLENDTSGQTLSDRVAAAGHALS
                     GRKIVKDDVRAIRHVMRDWTDRSDIDAIITTGGTGLTGHDVTIEAIRPLFDKEIDGFG
                     TLFHMVSFPKIGTSTIQSRATAGVVKGKYVFCLPGSPGACKDGWDLILKPQLDYTHKP
                     CNFVEIMPRLDEHLRRSKG"
     gene            complement(5195..>5888)
                     /locus_tag="AC16A0_00255"
     CDS             complement(5195..>5888)
                     /locus_tag="AC16A0_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3103785.1"
                     /translation="IVLISAFCVTIQGALLFVGLKGMAATSANLILQTQVPFAVILGW
                     LIGGEHLDLRKSIGTAIALAGVAIVIGLPEVPPPLVPTLLVIAGAFTWALGQVLARRL
                     GRDSGFGLLKLNAYGSVPQLALATLLFESGKIASLQSATWLEWSMLLFVGVVGFYLAY
                     LCWFAVLRQCRMDEVAPFILLMPVVGIATAFVLLGEAISPAQLLGGAVLLAGLAIVSG
                     VGLPKRRRAAAS"
BASE COUNT         1030 a         1937 c         1893 g         1028 t
ORIGIN      
        1 aagttccgtt cggcgatgtc ccactgcttc agccgctcat gcgcgatgcc gttgaagtaa
       61 tagatgcgcc agtggccggg cgtggtggtc tcctcggtca gaagcgcgat ggcctggtca
      121 tagaccttgg ccgcgttggc atagtcttcg ttgctgcgat agagattgcc cagcgtgacc
      181 atggcatcat agttgtcggg ctcgcgcgcg atgacgtcct tcagcttggc ctgcgcctcg
      241 tccttctttt cgaggcggtg aaggctgacg ccgatctcca tttcggcgtt ggtccggagc
      301 ggcgaggagg cggggatctc ggaataggcg gcgatcgcct tctcgtcgag cttcatgtcg
      361 cccaggatat cgcccaacag cgtgagcgtc accgcgcggt ccgcgttgaa ggacagcgcc
      421 agctgggtat agagcaaggc gacgtcgatg ctctgttcgt cggtcatcga ggtggccagc
      481 gagaacagcg cctccgcggc tccggcgccc ggcgttggta tgaatggcgc gggtttcttg
      541 ccggccttgg cgccgtcgag cgacgcctgg atgagcgggt tgtcgtcgcc ttcgagatag
      601 gtcgcgtaga tgcggatggc ctcgtcggcg cggccgctgc gctcgaggaa gttgccgtag
      661 ccctgcacga tgcgcagcga ggtcccggcc tgttcgtaag ccttcttgta gaagacctcg
      721 gcgcgaatgg cattgccgag gaaatcgctg atcagcgcgg cgtggaaatt cttgaagttg
      781 acgaaggcct cgttgcggtc caggcggtcc agcgccttga gcgcgagatt gagctcgcct
      841 tcgccggcat gggtccagcc caccagcagg ccggaggtca gttcgcccac cggcgtgtat
      901 gaggcgactt ccagattctt gcgcgcctcg gcatagcggc ggttgcggaa gtcgcgcagg
      961 cccagaacga tgcgcgccat gcgctgctgg ctgttgaagc cgaggacctg aatggcatat
     1021 tcctcggaat ccgcgatgtc gccttccgcc gtcttgagca ggaacacccg ctcgatgagg
     1081 acaggattgc ccggatcggc tttgagggcg cggacgaagt aatccgaggc caggtcattg
     1141 tcgcgcgcca cgctggccga gcgcccggcc aggtagttgc ccgacagcag ccggtcgtcg
     1201 agctgctcgt aggccgcggc gggttgggcg gtgaaggcgg tggcggcaag agccgccatc
     1261 atgaaggcgc gaagcgagga agccagcatt gatgtccagt tcgatgtggt gcacggcccg
     1321 atgccgcgca cgcgcggtga ctcatcttgt caaaatgagg cctttctggg gatgccgcaa
     1381 ctgctaaaag attaactctg ccgggctccg gcggcctcac atgttgggat agttcggccc
     1441 ttccccgccc tgcggcacaa cccaggtgat gttctgcgac ggatccttga tgtcgcaggt
     1501 cttgcagtgc acgcagttct ggtgattgat ctggaagcgc ggcgccttgc cctcctcgcg
     1561 caccacttcg tagacgcccg ccgggcagta gcgctgggct ggctcgtccc aggtcggcag
     1621 gttgaccgcg atcggcacgt cgggattctt cagccggagg tggaccatct ggtcctcctc
     1681 gtgattggtg gccgagaagg cgatgttggt caggcggtcg aaggagatca cgccatccgg
     1741 cttgggatag tcgatgggct tggcctcggc cgccggcacc agcgtctccg aatcgcgctt
     1801 gccgtgcttc caggtgccca gcccgaagcc cagcagctgg ttcatccaca tgtcgaggcc
     1861 gccaagggcg aggccgccca gcagccccag cttcgtccac atgggcttca cgttcttcac
     1921 gcgcttcagg tcggtatgga cccaggagtt gcgataagcc tcctcgtagg cagcgagctc
     1981 gtcgccgctg cgtccggccg cgagcgcggc aaaggccgct tccgcggcca gcatgccggt
     2041 cttcatcgca ttgtgcgacc ccttgatgcg cggcacgttg acgaagccgg ccgagcagcc
     2101 gatcagcgcg ccgcccggga agtagagctt cggcaccgac tggaacccgc cctcgttgat
     2161 ggcgcgcgcg ccataggcga tgcggcgccc gccctcgagc accggcctga tcagcggatg
     2221 atgcttgaag cgctggaatt ccatataggg aaacaggtgc gggttcttgt agttcaggtg
     2281 caccacgaag ccgatcgaga cgagattgtc ttccagatga tacatgaagg aaccgccgcc
     2341 cgccttcatg ccgagcggcc agcccatggt gtgggtcacc tgaccctgct tgtgcacctc
     2401 ggggcgaacc tcccagagct ccttcatgcc gaggccgtac ttctggggct cccggccata
     2461 ggcgagatcg tatttggcga tgatctcctt ggccagcgag ccgcgcacgc cttcggcgat
     2521 gaagacatat ttgccgagca gttccatgcc aggctggaag tcggccttgg gctggccgtc
     2581 cttgccaagg cccatcacgc cggcgaccac cccccggacc gagccgtcct cgcggtagag
     2641 cagttcggaa caggcgaaac cgggatagat ttccacgccc aggccttccg cctgctgcgc
     2701 cagccagcgg cagacattgc ccagcgacac ggcatagttg ccgtgattgt ccatcagcgg
     2761 cggcatcatg aaattgggca ggcgcagctg gcccgaggga ccgagcatgt agaaacggtc
     2821 ctcggtgacg ggcgtgttga gcggcgcgcc cagttccttc cagttcggaa tgagctcgtt
     2881 gagcgcgatc gggtcgatca ccgcgccgga aagaatatgc gcgcccacct ccgaaccctt
     2941 ttcgagcagg cagacgctga tctcggggga cagctgcttc agccggatcg ccgcggcaag
     3001 gccggcgggg cctgcgccca cgatcacgac atcatattcc atggcctcgc gctggatctg
     3061 ttccacagac ataactcccg cctatcgctt gctcctgcgg gcgtttatat tgacggaatt
     3121 ctccacacaa gccgccaatt cggacatgcc acggaaccaa agcgacatga cacccgcgac
     3181 ggccgctgcc gccttggcct ggctggccga gatgggcgcc gacgagatca tcggggatac
     3241 gcccgtgaac cgcatggttg ccgcacccgc acctgccgcg gcaaccgtga ccgcggcaag
     3301 gccggccgcc gccgtcgtgc aggcagcgcg caagggtgag gcgccggccg accccgcaag
     3361 ctgcagcacg cttgcggtga tcgaagcgtc gctgtccgcc ttcgacgcct gcccgctcaa
     3421 gaagaccgcc accaatctct gctatgccga cggcaatccg tccgcccgcg tgatgctggt
     3481 gggcgaggcg ccgggccgcg acgaagacat ccagggcaag cccttcgtcg gacgctccgg
     3541 ccagctgctc gaccgcatgc tggccgccat cgggctggac cgcaaggcgg acgatcgcga
     3601 aagcgccgtc ttcatcacca atacgatctt ctggaggccg ccaggcaacc gcacgccgac
     3661 cgaggccgag accgccatgt gcctgccctt cctgctcagg accatcgaga ttcagaaacc
     3721 cgacgtgatc gtctgtctcg gcgccacgcc ggcgcaccgg ctgacgggca ggagcgacgg
     3781 catcctcaag ctgcgcggca aatggaccga agcaaccgtc agtggacgga agatcccgct
     3841 gctgcccacg ctgcacccgg cctacctgct gcgccagccc gcccagaagc gcctggcctg
     3901 gcgcgatctg ctgtccctgc gccaggcgct cgatgcccat tgagtggccc gacctgctgc
     3961 tgttcctgtc ggcatcgctg atgctgaatc tcacgccggg caacgacatg atgttcgtcc
     4021 tcggacagtc gctgcgcggc ggcacacgca gtggaatcgc cgccagcctc ggcatcgcaa
     4081 cgggatcgct ggtgcacctc gcgctggtgg cgatgggcct tgccgtggtg ctcgcccgca
     4141 atccggccgt cttcgaggcg atccgctatg cgggggcggc ctacctcgtc tacatcgcca
     4201 tccagaccct gcgcagcggg ccggcgattc tgaagcccgc gccgggcgat gcgagcgcct
     4261 tcaaggcatg gcgcgacggc gccgtggtga acatcctgaa ccccaagatc atcgtattct
     4321 tcttcgcctt ccttccgccc ttcgtgaaac ccgagaacgg gcaaccgctg ctgcagcttg
     4381 tcatcttcgg gctgctgttc aacataggcg gtacactgat caatttcgcc gtggctgcct
     4441 tcgcggcacg ccttgcaggc gttttctccg gtaacgtcaa gatcgcacgc gcattcgctg
     4501 tgctctcgtc agcacttttt cttgtcctcg cagcgcgaat gttgtttgat cgcaaggcct
     4561 gaagacacgc aaattttacg tatcttgcgc attgcgtaac tttgcggagg cccgcgcggc
     4621 ggcggaatgg attttgagga cgaaacgtta tgtcagaggc ccgcagcttc attccggtga
     4681 ggatcgcaat cctcaccatt tccgatacgc gtacgctgga gaacgacacg tcggggcaga
     4741 ccctgtcgga ccgcgtcgcg gcggcaggcc atgcgctgtc gggccgcaag atcgtgaagg
     4801 acgatgtccg cgccatccgc cacgtgatgc gcgactggac ggaccgcagc gacatcgacg
     4861 ccatcatcac caccggcggc acgggactga ccggacatga cgtgacgatc gaggccatcc
     4921 gtccgctctt cgacaaggag atcgacggct tcggcacgct cttccacatg gtctcctttc
     4981 cgaagatcgg aacctcgacc atccagtcgc gcgccaccgc cggcgtggtg aagggcaagt
     5041 atgtcttctg cctgccaggc tcgcccggtg cctgcaagga cggctgggac ctgatcctca
     5101 agccccagct cgactacacg cacaagccct gcaacttcgt cgagatcatg ccgcgcctcg
     5161 acgagcatct gcggcgcagc aagggctgag ccgctcagga cgcggcggcc ctgcgccgct
     5221 tcggcagccc gacgcccgag acgatcgcca gtccggcgag cagcaccgcg ccgcccagca
     5281 gctgggcggg cgaaatggcc tcacccagca gcacgaaggc ggtggcaatg ccgaccaccg
     5341 gcatcagcag gatgaagggc gccacctcgt ccatgcggca ctggcgcagc accgcgaacc
     5401 agcagagata ggcgagatag aatcccacca ccccgacgaa aagcagcatc gaccattcca
     5461 gccaggtggc gctctgcaat gacgcgatct ttccggactc gaacagcaga gtcgccagcg
     5521 ccagctgcgg cacgctgccg taggcattga gcttgagcaa gccgaagccg gagtcccggc
     5581 ccagcctgcg ggccagcacc tggcccagcg cccaggtaaa ggcgcccgcg atcaccagca
     5641 gggtcggcac cagcggaggc ggcacttcgg gcaggccgat gacgatggcg acgcccgcca
     5701 gcgcgatggc cgtgccgatg ctcttgcgca ggtcgaggtg ttcgccgccg atcagccagc
     5761 caaggatcac ggcgaagggc acctgggtct gcaggatgag attggccgag gtcgccgcca
     5821 tgcccttcaa tccgacgaag agcagcgcgc cttggatggt cacgcagaag gccgagatca
     5881 gcacgatc
//
LOCUS       DBNGVR010000011         3028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2951673, whole genome shotgun sequence.
ACCESSION   DBNGVR010000011 DBNGVR010000000
VERSION     DBNGVR010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2951673"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="AC16A0_00260"
     CDS             <1..891
                     /locus_tag="AC16A0_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003595069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3103786.1"
                     /translation="SYIAELKAQGENVNTEQIEALRAQYGFDQPVLIRYFDWVWGMLH
                     GDFGYSFEYQLPVNEVVGDRLWLTMLVSFVTIVFTWLIAFPIGIYSATHQYSWGDYGL
                     TFLGLLGLAIPNFMLALILMYFANIWFGTSIGGLMDREFLAEPMSWAKAKSIMSHLWI
                     PVIIVGTAGTAGMIRRLRANLLDELRKQYVTTAKAKGVPPMRALVKYPLRMSLNFFIA
                     DIGSILPAIISGAEITAIVLSLETTGPMLIRALQSQDMYLAGSFLMFLAFLTVIGVLI
                     SDLALAALDPRIRLQGTSTK"
     gene            888..2063
                     /locus_tag="AC16A0_00265"
     CDS             888..2063
                     /locus_tag="AC16A0_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018209807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3103787.1"
                     /translation="MTSPLPAPGAPLHHYVSNAPFDPHTITAMTDEQSKVHLASQWKL
                     MWWKFKRHRVAVYCGIFLALMYASTLFSEFLAPYNLHTRNVEAIHAPPQRVHLFHEGS
                     FVGPFVYGRERSLDMNTMKRVYTDTTNVIHPVRFFCKGDAYKFWGIWDADRHLFCPAE
                     GGTLFLLGTDRLGRDVLSRIIYGARISLTIGLIGITVSFALGIIIGGLAGYHGGWFDL
                     AVQRVIEVLQSIPSIPLWMALAAIMPVSWSPIVIYFGITFILGLLDWTGLARAVRSKL
                     LSLREEEYVLAAQLMGAKDSRIIGRHLIPGFISHLIATATIAIPGMILGETALSFLGL
                     GLRPPITSWGILLTEAKNVSVVALYPWLLFPMVPVILVIIAFNFFGDGLRDAADPYN"
     gene            2143..2931
                     /locus_tag="AC16A0_00270"
                     /pseudo
     CDS             2143..2931
                     /locus_tag="AC16A0_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
BASE COUNT          552 a          937 c          868 g          671 t
ORIGIN      
        1 agctacatcg ccgaactcaa ggcccagggc gagaacgtca acaccgaaca gatcgaggcg
       61 cttcgggccc agtacggctt cgaccagccc gtcttgatca ggtatttcga ctgggtctgg
      121 ggcatgctgc atggcgactt cgggtactcc ttcgagtatc agctgccggt gaacgaagtc
      181 gtgggcgacc ggctctggct gaccatgctg gtctccttcg tgaccatcgt cttcacatgg
      241 ctgattgcct ttccgatcgg catttattcg gcaacccatc aatacagctg gggcgactat
      301 ggcctgactt tcctgggatt gctcgggctt gcgatcccga acttcatgct ggcactcatt
      361 ctcatgtatt tcgccaacat ctggtttggc acgtcgatcg gcgggctgat ggaccgcgag
      421 ttcctggcgg agcccatgag ctgggccaag gcgaagtcga tcatgagcca cctgtggatt
      481 ccggtgatca tcgtgggaac cgctggcaca gccggcatga tccgccgctt gcgcgccaat
      541 ctgctggatg aacttcgcaa gcagtatgtc acgacggcaa aggcgaaggg cgtgccgccc
      601 atgcgggccc tcgtgaagta tcctctgcgc atgtcgctga atttcttcat cgccgacatc
      661 ggttcgatcc tgcccgcgat catctcgggc gcggagatca ccgctatcgt gctgtcgctg
      721 gaaaccaccg gacccatgct gatccgtgcc ctgcagagcc aggacatgta ccttgccggt
      781 tcgttcctga tgttcctcgc cttcctcacc gtcatcggcg tcctcatctc ggatctggcg
      841 ctggcggcac ttgatccgcg catccggcta cagggcacga gcacaaaatg acatcgcctc
      901 tgccggcacc cggcgctccg ctccatcact atgtctcgaa cgcaccgttc gatccccaca
      961 cgatcacggc gatgaccgac gagcagtcga aggtccacct cgcgtcgcaa tggaagctca
     1021 tgtggtggaa gttcaagcgc caccgggttg ccgtctactg cgggatcttt ctggcgctca
     1081 tgtatgcctc gacactcttc agtgaattcc tggcgcccta caatcttcac acacgcaacg
     1141 tcgaagccat tcatgcgccg ccgcagcgag ttcacctttt ccatgagggg agctttgtgg
     1201 gtccattcgt atatgggcgg gagcgcagcc tcgacatgaa caccatgaag cgggtctata
     1261 ccgacactac gaacgttatt catcccgtcc ggttcttctg caagggcgac gcctacaagt
     1321 tctggggaat ttgggacgcc gaccgccacc tgttctgccc ggcggagggc ggaacgctgt
     1381 tcctgctcgg gaccgatcgt cttggacgcg acgtcctgtc gcgcatcatc tatggcgcgc
     1441 gcatttccct gaccatcgga ctgatcggca taaccgtgag tttcgccctg ggcatcatca
     1501 tcggaggcct ggccggctac cacggcggct ggttcgactt ggccgtgcag cgcgtcatcg
     1561 aggtgctgca gtcgatcccg agcattccgc tgtggatggc gctggctgcg atcatgccgg
     1621 tgtcgtggag tccgatcgtg atctattttg gcatcacctt cattctcgga ctgcttgact
     1681 ggacaggcct cgcgcgtgcg gtgcgctcca agcttctgtc gttgcgtgaa gaagagtatg
     1741 ttcttgcagc tcagctgatg ggcgccaagg acagccgcat catcggccgt cacctgatcc
     1801 cgggtttcat ttcgcacctg atcgcgacgg cgaccatcgc cattcccggc atgatccttg
     1861 gcgagacggc tctcagcttc ctggggctcg gcctgcggcc cccgatcacc agctggggca
     1921 ttctgctgac agaggcgaag aacgtcagtg tcgtcgcgct atatccatgg cttctctttc
     1981 caatggttcc tgtgattctc gtcatcatcg ccttcaattt ctttggcgac gggctgcggg
     2041 atgccgccga cccttacaac tgaatcgagg tatcgccgtg ataactgttc ggaaggcagg
     2101 atcggtgaat tcggagcgtt cgggtctttc gagggacccg ttgtgacgcg gcgctatgac
     2161 aatacccgca tcctgatgta tagccatgac acgttcgggc tgggtcactt gcggcgatgc
     2221 cgtgccatcg cccattctct cgtcgagcac gcgcgcggat tgagcatcct catcatttcg
     2281 gggtccacca tcgccggcgc cttcgactac cgcgcgcgcg tcgacttcgt gaagattccg
     2341 agcgtgatca agttgcgcaa cggagaatat acttccctgg accggcacat cgatctgcag
     2401 gaaacgctga tgatgcggca gtcgatcatt cgccacacgg cagatacgtt tcagcccgat
     2461 gttttcattg tggacaagga acctcttggt ctgcgcggcg aagtcgaaga caccctggcc
     2521 tacctgaaaa ccaagggcac cattctcgtt ctcggtttac gcgaagtgat ggacgcgccg
     2581 gacctcctcg atgccgagtg gaaacagcgc gatgtgctcc gcaagatcgg caggctctat
     2641 gatgcgatct gggtttatgg accgcctgac ttctacaacc ctctcaccgg cctcgacgtg
     2701 ccggagttgg tcagctcaaa gctcgagtat gtcggctttc tgcagcgcga acttaatgcc
     2761 gaagagatcc cgggccaccg accggagggc gactacatac tggtgacacc cggcggcggc
     2821 ggcgatggag ccgaattgat ccacaacgtc ctgcatgcct accagcagga tccggaactc
     2881 acctacaagg ccgtcatcgt gcttggcccc tacatgccgg cgcgctcgag gccaagcagt
     2941 tcctgcgcga tgaaggtttc cgacaggcgc ggatagccct tcaggacgat gacggtcttc
     3001 ttcacctctt tacgcgactt cagctgag
//
LOCUS       DBNGVR010000012         1924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1969765, whole genome shotgun sequence.
ACCESSION   DBNGVR010000012 DBNGVR010000000
VERSION     DBNGVR010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1969765"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1254
                     /locus_tag="AC16A0_00275"
     CDS             145..1254
                     /locus_tag="AC16A0_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3103788.1"
                     /translation="MATLIRKLVIGTVAAVLVTAGGLAQAQQVTRMKIQTAVPTASIY
                     FDLMKRFGDRVDKMSNGRIKMDILPDGSVVNAFEILDAVDKGVVDGGFAWTHYWSGKN
                     SAAALFSNPAAGSGTGMDQLSHMAWLSQGGGNALYEKLYESGLKLNIKPFMMQPMGPD
                     PFGWFKTPINSIADMKKMKYRSPPGLTGEIFKEMGINAVAMPGGEIVPAAQRGVIDAA
                     EWIGPADDMILGFHNVFKHYYLQGLHQSTDVGEILINKTVWNKLPADLKAVIEGAVMA
                     TITETYAFNVDRNAKALEKLQKEHKVTVHDTPKEFFAAFLKATNLVYDREAARNPLFK
                     EVLESQRAYAKVVVPYWTKINGLYYGLGAEAVGSK"
     gene            1335..1901
                     /locus_tag="AC16A0_00280"
     CDS             1335..1901
                     /locus_tag="AC16A0_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR3103789.1"
                     /translation="MTGQPSKLLRIAHALDPIAIWSGKLTAWLILPMVLSLAYEVTAR
                     YVFDSPTLWAYDMTFMLYGAYFMLGAAYTLQRKGHIRTDSFYGAWSPRRQGIVDAICY
                     LVMFMPFVAIFLWFGWGYFLKAFMTGERFVSSPWMPITWPFKLAMPLTGLLLAIQGVS
                     ELCKSLDAAISGRWADGGVSALPGKEAA"
BASE COUNT          379 a          591 c          591 g          363 t
ORIGIN      
        1 aatgattgcc gagaaggccg ccgacatgat tcgtgaggat gccggccagt aagggatttg
       61 tggattgccc atgaggcaat ggtggagtcc ttgcgatccg acggatccgg gggcccggtt
      121 tttaatataa cggaggaggc agtgatggcg actttgatac ggaaactggt gatagggact
      181 gtcgcggcgg tgctggtcac cgcaggcggc ctggcgcagg cccagcaagt gactcgcatg
      241 aaaatccaga cagcggtccc gaccgcgagc atctatttcg acctgatgaa gcgttttggc
      301 gatcgtgtcg acaagatgtc caacggccgc atcaagatgg acatcctgcc ggacggctcg
      361 gtggtcaacg ctttcgagat cctcgacgcg gtagacaagg gcgtggtcga tggcggtttc
      421 gcctggacgc actattggtc gggcaagaac agcgcggcag ccctgttctc gaacccggcg
      481 gcgggctccg gcaccggcat ggaccagctg tcgcacatgg cctggctgtc gcagggcggc
      541 ggcaatgccc tttacgagaa gctctacgag tccggcctga agttgaacat caagcccttc
      601 atgatgcagc ccatgggtcc cgatcccttc ggttggttca agacgccgat caactcgatc
      661 gccgacatga agaagatgaa gtaccgctcg cccccgggac tcaccggcga gatcttcaag
      721 gaaatgggca tcaacgcggt ggccatgccg ggcggcgaga tcgttccggc cgcgcagcgt
      781 ggcgtgatcg atgccgccga gtggatcggc ccggcggacg acatgatcct cggcttccac
      841 aacgtgttca agcactacta cctgcaggga ctgcaccagt cgaccgacgt cggcgagatc
      901 ctgatcaaca agacggtctg gaacaagctg ccggcggacc tgaaggccgt gatcgagggc
      961 gcagtgatgg ccaccatcac cgagacctat gccttcaatg tcgatcgcaa tgcaaaggcg
     1021 ctggaaaaac tgcagaagga gcacaaggtc accgtgcatg acacgccgaa ggagttcttc
     1081 gcggccttcc ttaaggccac caacctcgtc tatgaccgcg aggcggcacg caatccgttg
     1141 ttcaaggaag tcctcgaatc gcagcgcgcc tatgccaagg tcgtggtgcc ctactggacc
     1201 aagatcaacg gcctgtatta cggcctcggc gccgaagcgg taggcagcaa gtaagtctca
     1261 atccggcccg gacggtgcac ggcgtctgcc gtgcaccgtg cctgcctcca ggatcaccca
     1321 cggatacacg agccatgacc ggacaaccct caaaactgct gcgcatcgca cacgcactcg
     1381 accccatcgc catctggtcc ggcaagctga ccgcatggct gatcctgccc atggtgttgt
     1441 cgctcgccta cgaggtcacg gcgcgctacg tgttcgattc cccgacgctg tgggcctatg
     1501 acatgacctt catgctgtat ggcgcctact tcatgctcgg cgcggcctac accttgcagc
     1561 gcaagggaca catccgcacc gattcgttct acggcgcatg gtcgccgcgt cgccagggca
     1621 tcgtcgatgc catctgctac ctggtgatgt tcatgccctt tgtggcgatt tttctctggt
     1681 tcggctgggg ttacttcctc aaggccttca tgaccggcga acgctttgtc agcagcccgt
     1741 ggatgccgat cacctggccg ttcaagctgg ccatgccgct gaccggtttg ctgctggcga
     1801 tccagggcgt ctccgaactc tgcaagagcc tcgatgccgc gatcagcgga cgctgggccg
     1861 acggcggggt ctcggcactc cccggcaagg aggccgcatg agcaacgaac tcttcggcct
     1921 gctg
//
LOCUS       DBNGVR010000013         4246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3540952, whole genome shotgun sequence.
ACCESSION   DBNGVR010000013 DBNGVR010000000
VERSION     DBNGVR010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3540952"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..260)
                     /gene="hfq"
                     /locus_tag="AC16A0_00285"
     CDS             complement(15..260)
                     /gene="hfq"
                     /locus_tag="AC16A0_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR3103790.1"
                     /translation="MAQEKQQNLQDTFLNHVRKQKIPVTVFLVNGVKLQGVITWFDNF
                     CVLLRRDGMSQLVYKHAISTIMPGGPISLFDEESAGH"
     gene            complement(335..1720)
                     /gene="ntrX"
                     /locus_tag="AC16A0_00290"
     CDS             complement(335..1720)
                     /gene="ntrX"
                     /locus_tag="AC16A0_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation response regulator NtrX"
                     /protein_id="HIR3103791.1"
                     /translation="MAADILIVDDETDIRELIAGILQDEGYETRLAHNSDAALSEVAA
                     RRPSLIILDIWLMGSKLDGLDLLNVIKEQHPEVPVIIISGHGNIETAVAAIKRGAYEY
                     IEKPFKADRLLLVVGRALETFRLRRENEALKGKAGADTELLGSSNAMRQLRQLLKKIA
                     PANSRVLITGPMGAGKELAARTLHSLSLRANGPFVTLSAATMAPERMEEELFGIEDDE
                     GAARRVGALEEAHGGTLYIDELADMPLETQGKVLRVLVDQTFQRVGGSKKVKVDVRVV
                     SSTSRDLPGLIVDGHFREDLYHRLGVVPVRVPGLSERREDIPELVEHLARLYSLSSGQ
                     PVRRFAGDVIAMLQTRDWPGNVRELKNNVERFMILAAGDPASEVTMDSLPSDASTSFS
                     ASGSGIEHLLVHPLREAREAFERDYIAAQLSRFGGNISKTSQFIGMERSALHRKIKLL
                     GLSARNEDETA"
     gene            complement(1733..3946)
                     /locus_tag="AC16A0_00295"
     CDS             complement(1733..3946)
                     /locus_tag="AC16A0_00295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF039586.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase NtrY-like"
                     /protein_id="HIR3103792.1"
                     /translation="MADRSPSRLTTRLRRLTGWAGFLLVVLGVVVGMATFAILTGLTP
                     LKPTPQTTMLLLTVNGVVMAVMALMIIGQLIFLIAERRQGTPGAGLHLRLVGLFSAMA
                     IIPAILVAVFASVTLSRGLDTWFSERTRAIVDAAVNVAEAYLRDHAEATRGDVAAISA
                     DLAQNKEMFEKDRPSFVRRVARHAALRGLAGVFLFNPEYKRIDVKVTANDKVQFFAPT
                     EEQLNKAKAGELLVIQPGLGGNVVRALMKLPDYNNEFLYVYRVVNPSVIDQLAKTREA
                     KQEYDRLMSQRLGVQLTFALMYGVVSFVFLLAAIWTGLWFSDRLVAPIVRLLDASRRV
                     TAGQLDAKVEVIEGPPDLQTLSSTFNLMTGRIKRQRDELVEANEAVDDRRRFTEAMLA
                     GVSAGVIGIDPGGNISLVNRSALNLLSMTEEDLMGKPVAAALPHFQDVFQQAWSRPSG
                     FSEGQVDLQLHGESRSFFARVTTEAADAEQHGHVLTFDDVTDLVSAQRNSAWADIARR
                     IAHEIKNPLTPIQLSAERLKRKYSKQIINDKQIFDQCTDTIIRQVGDIGRMVDEFSSF
                     ARMPSAAPEPQNLAAVVREATVLQRVSSSDIDIELEVRGGEFVFPFDRRLITQGITNL
                     VKNAREAIESRQQSQPEPRGRILVEAGLEQGHPFVRVTDNGVGLPKENRHRLAEPYMT
                     TREKGTGLGLAIVKRIMEEHGGRLVLEDVPSTDGATGARVTLLFDQVPVTMEEAV"
     gene            complement(4009..>4246)
                     /locus_tag="AC16A0_00300"
     CDS             complement(4009..>4246)
                     /locus_tag="AC16A0_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3103793.1"
                     /translation="LPEFMENYLSRYFAGFGQSLPPSGLYERILSEVEPALLRAALAA
                     TSGNQLRAAELLGINRNTLRVKLRERNVRVIRGL"
BASE COUNT          807 a         1307 c         1305 g          827 t
ORIGIN      
        1 ccgcttccgt gtgctcaatg tcccgccgat tcttcgtcga acaggctgat cggaccgccc
       61 ggcatgatgg ttgaaatggc atgcttgtag accagctgcg acatgccatc acggcggaga
      121 agcacacaga agttgtcgaa ccaggtgatc acgccctgaa gcttcacgcc gttcaccaga
      181 aataccgtga ccgggatctt ctgcttgcgc acatggttca ggaacgtgtc ctggagattt
      241 tgctgttttt cttgggccat ttgctcttct cgtttttggg ccggttgcgg cggccaaggc
      301 tatcacaaaa ccgccggaag ccaaatcagc cggctcacgc ggtctcgtcc tcgttacgcg
      361 cggaaaggcc tagcagtttg atcttccggt gcaacgccga acgttccatg ccgatgaatt
      421 gggacgtttt cgagatgttg ccgccgaaac gcgacagctg ggcggcgatg tagtcgcgct
      481 cgaaggcctc ccgggcctcg cgcaagggat gaaccagaag atgctcgatc cccgagcccg
      541 aagccgaaaa cgacgtgctg gcgtcacttg gcagcgaatc catggtcacc tcgctggccg
      601 gatccccggc cgccaggatc atgaatcgtt cgacattgtt cttgagttcc cggacattgc
      661 ccggccagtc cctcgtctgc agcatggcga tgacgtcgcc ggcaaatcgc cgcacgggct
      721 ggccggacga cagggagtaa agcctggcca ggtgttccac cagttccgga atgtcttcac
      781 ggcgttccga caatccgggc acccgaaccg gaaccacgcc gagccggtga tagagatctt
      841 cccggaaatg accatctaca atgaggccag gcaggtcgcg gctggtcgag gagaccaccc
      901 gtacgtcgac cttcaccttc ttggagccgc ccacgcgctg gaacgtctgg tccaccagaa
      961 ctctgagaac ttttccctgt gtttccagag gcatgtctgc gagttcgtcg atgtagagcg
     1021 ttccgccatg agcctcctcg agggcgccca cgcggcgtgc cgcgccctcg tcatcctcga
     1081 tgccgaaaag ctcctcctcc atgcgctccg gcgccatggt tgcggcactc agcgtaacga
     1141 aggggccatt ggcgcgaagc gacagcgaat ggagcgtccg tgcagccaac tctttgcccg
     1201 cccccattgg gccggtgatc agcacgcggc tgttggcggg cgcgatcttc ttcagcagct
     1261 gccgcagctg ccgcatggcg ttcgaactgc ccagcagctc ggtgtcggct cctgctttgc
     1321 ccttgagcgc ctcgttttcc ctcctcaggc ggaaggtttc gagcgcgcgg ccgacaacca
     1381 gcagcagacg gtccgccttg aagggtttct cgatgtactc gtaggcacca cgcttgatgg
     1441 ctgcgacggc cgtttcgata ttgccgtggc cggagatgat gatgacagga acctcggggt
     1501 gctgctcctt gatgacattg aggagatcga ggccgtcgag cttcgatccc atcaaccaga
     1561 tgtcgaggat gataagggac ggccgacgcg ccgcgacttc agaaagtgcc gcgtcgctgt
     1621 tgtgggcaag acgcgtctcg tacccttcat cctgcagaat gcctgcgatc agctcacgga
     1681 tatcggtttc gtcgtcgacg atcagaatgt cagcggccat aagcaactct catcacactg
     1741 cctcttccat cgtcaccgga acctgatcga agagcaaggt cacacgcgcg cccgttgccc
     1801 cgtcggtgga cggaacatct tcgaggacga ggcgcccacc gtgttcctcc atgatgcgct
     1861 tgacaattgc gagacccagc ccggtcccct tctcgcgcgt tgtcatatag ggttctgcca
     1921 gacgatgacg gttttccttg ggcaggccga cgccattgtc tgtgacacgg acaaagggat
     1981 gcccctgctc gagcccggct tcgacgagaa tgcggccccg cggttccggc tggctctgct
     2041 ggcggctctc gatcgcctct cgcgcgttct tcacgagatt ggtgatgccc tgggtgatca
     2101 ggcggcggtc aaaggggaaa acgaactcgc cgccgcgcac ctccaattcg atatcgatat
     2161 cggaagagga cacacgctgc agaactgtcg cctcgcgcac cacggcggcc aggttctgcg
     2221 gctccggcgc tgccgaaggc atgcgcgcaa aggacgagaa ctcgtcgacc atcctgccga
     2281 tatcgccaac ctgcctgatg atcgtatccg tgcattgatc gaagatctgc ttgtcgttga
     2341 tgatctgttt gctgtacttg cgcttcagcc gctcggcgga aagctggatt ggcgtgagcg
     2401 ggttcttgat ttcatgggca atgcgccgcg caatgtcggc ccaggccgag ttccgctgcg
     2461 ccgacacgag gtcggttaca tcgtcgaacg tgaggacatg gccgtgctgt tcggcatccg
     2521 cggcctcagt ggtgaccctg gcaaaaaagc tccggctttc gccatggagt tgcagatcga
     2581 cctggccttc cgagaacccg gaaggcctgg accaggcctg ctggaacacg tcctggaagt
     2641 gcggcagcgc tgcggccact ggtttgccca tcaggtcttc ttccgtcatg gagagaaggt
     2701 ttagggcaga ccggttcacg agtgagatat tgccgccggg gtcgatgccg atgactccgg
     2761 cgctgacgcc agccagcatg gcttcggtga agcgccggcg gtcgtcgacg gcttcgttgg
     2821 cctcaaccag ttcgtcacgt tgccgcttga tgcggcccgt catcagattg aaggtgctcg
     2881 acagggtctg aaggtcgggc ggtccctcga tgacttccac ctttgcatcc agctgaccgg
     2941 ccgtcacgcg tcgcgaggca tcgagcaggc gcacgatcgg ggccaccagc cggtcggaga
     3001 accaaaggcc ggtccagatt gcggcgagga ggaagacgaa gctgaccacg ccatacatca
     3061 gtgcgaatgt gagctgcacg cccagtcgct gcgacatgag cctgtcgtac tcctgcttcg
     3121 cctcgcgcgt ctttgccagc tggtcgatga ccgacgggtt caccacgcgg tagacataga
     3181 gaaactcgtt gttgtagtcc ggcagcttca tcagggccct gacgacattg ccgcccagtc
     3241 ctggttggat gaccagcagc tcgccggctt tcgccttgtt cagctgctcc tctgtcggtg
     3301 cgaagaactg gaccttgtca ttggccgtga ccttgacatc gatgcgcttg tactcggggt
     3361 tgaacagaaa gacgccggcc aagccgcgca acgcggcatg gcgcgccacc cggcggacga
     3421 aggacgggcg gtccttctcg aacatctcct tgttctgggc cagatcggcc gaaatggccg
     3481 caacgtcgcc gcgggtggct tcggcatggt cacggagata ggcctcggcg acattgacag
     3541 cggcgtcgac gatggccctg gtgcgttccg agaaccatgt gtccaggccc cggctgaggg
     3601 tcacggaggc aaacacggcc accaggatcg ccggaatgat ggccatggcg ctgaacagcc
     3661 cgaccagcct gagatggagt cccgctccag gcgtcccttg ccgccgttcg gcaatcagaa
     3721 agatcagctg gccgatgatc atcagcgcca tgacggccat gaccacgccg ttgaccgtca
     3781 gcagcagcat ggtggtctgg ggcgtcggct tgagcggcgt aaggccagtc agaatggcaa
     3841 aggtggccat gccgacaacg acgccgagaa cgaccagcag gaatccagcc cagccggtca
     3901 gccgccgaag tctcgtggta agccttgagg gtgagcgatc agccatggca acagttcaga
     3961 gcaactgttg catgaaatca acactaaatg tggcagagtt gcgacagtct acaggccgcg
     4021 gatgaccctg acgttgcgtt cccggagctt tacccgcagc gtattccggt tgatgccaag
     4081 cagttccgct gcgcgcagtt gattgcccga ggttgcagca agcgcggcgc gcaacaaggc
     4141 gggctccacc tccgacagaa tacgctcata gaggccggag ggcggcaggc tttgcccgaa
     4201 gccggcaaag tagcgcgaca gatagttctc catgaattcc ggcaag
//
LOCUS       DBNGVR010000014         4394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4916464, whole genome shotgun sequence.
ACCESSION   DBNGVR010000014 DBNGVR010000000
VERSION     DBNGVR010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4916464"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1119)
                     /locus_tag="AC16A0_00305"
     CDS             complement(154..1119)
                     /locus_tag="AC16A0_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SphA family protein"
                     /protein_id="HIR3103794.1"
                     /translation="MTTRTVLIAQAAALTAVFSSPAMAVEGGTSFYLLGSKTTMAGFL
                     PPPGFYGLLQNYAYTGSANIDFETAGISLSGGVEADAYIALPTALWVTGTDVPGGNLA
                     FTLTTPFGGKRVEVGLITGRGGEADAERDNWAFGDPVLGATLGWHDGNLHYSLGTLIN
                     VPIGQWELGNPVNIGFNRWVIDTTAALTYLDQTTGLELSGAVGLTYNFENPDTDYKSG
                     TELHAEGAAMYHASHSLSIGVNGYALKQITGDSGSGAVLGDFEGQVFAIGPALDYTFM
                     IGQRPVATNLRYFYEFGVENRMQGHAGFLNIALPLGGHSAEEQGK"
     gene            complement(1185..1514)
                     /locus_tag="AC16A0_00310"
     CDS             complement(1185..1514)
                     /locus_tag="AC16A0_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103795.1"
                     /translation="MKLRKPTRYLVAALALGMICGDVEIAGQQASIGFFKLIGDAEAR
                     VGRPATPGSVAGVARRTTRRTIRRGAYIAAIPAGCAYGSYYGYSLYYCGGTYYQKSGG
                     GYVIVYF"
     gene            complement(1511..2290)
                     /locus_tag="AC16A0_00315"
     CDS             complement(1511..2290)
                     /locus_tag="AC16A0_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HIR3103796.1"
                     /translation="MTIYRLKLFAAVVAGLAFAVSPASAEQSEAKAVFKAMSDFLTAQ
                     QSLSVGYDATLEIVTAEMMKVGLASSGNLSFSRPDKIRMTRTGGIADVEMVYDGKTLS
                     AYGKRLNVFGKLPLEGKLDDVIAMLRDEFGLELPAADLLSSAPYEVMMENVIDAKALG
                     EGMIRGQMCDHLAFRTSDVDWQIWVAQGEKPFPCRFTITSKMTALAPSYSIEFSGWKT
                     GAEIAADDFQLKPADGAKEVAITELKGLDEVPAPASEGDAQ"
     gene            complement(2454..3470)
                     /locus_tag="AC16A0_00320"
     CDS             complement(2454..3470)
                     /locus_tag="AC16A0_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR3103797.1"
                     /translation="MPSFDTREFTLRVGPATCLPALARSFGRDPAPALAAADLDERLF
                     ASPENRVSIVKLGKFASGIARLIERPDLGLLIAKSYGPHSLGLILTLAEEGPDVRTAL
                     LNIARLLKHHNEFAFLSLSEQGSDAVLSYELRETDFEGEDIVLVTAIGNAFRVMRRFC
                     GDDWCPAEVRLSMARPKNVTPYETFFGAPVRFESAMDALVFTRHWLDQPVTPELGARG
                     GGSLPPSAWDFTDHVRHQIAMRIGREPVTARAIAAAMDLSRRTLDRRLSGHGVSFSQL
                     VDEIRFARARRLLAAGHAPLSDISLALGYAEPSAFSRAFRGWAGCSPQDWRLSREGAQ
                     PTEM"
     gene            complement(3914..>4394)
                     /locus_tag="AC16A0_00325"
     CDS             complement(3914..>4394)
                     /locus_tag="AC16A0_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3103798.1"
                     /translation="RPPRPPDEPLFSVPTILWSLVQGLFVLGVTGMVFLLAPQYGLEA
                     DQVCGLTFASLVLCIMALILVDRSQSSSIITAIRRPNRALAVVVPIVICLLAATLFWQ
                     PARQLFGFGVLEPLLLAVPPLAGLAVLLVLEALKPIWRRAVRPRASSYALLSTGRHP"
BASE COUNT          944 a         1378 c         1309 g          763 t
ORIGIN      
        1 ccgctgttcc gccgccagaa ccggcatgca gaaggcgatc agaatcgcca cggcggcaag
       61 cagcggcgtc atcaacctca aatccgaact accaaaccac gatgccaaca ttgtctcact
      121 ccgaagaagc ccagaagctc ttcctgtgtg aagttacttc ccctgctctt cggccgaatg
      181 cccgccgagc ggcaacgcga tgttcaggaa cccggcatgc ccctgcatgc ggttctcgac
      241 gccgaactcg tagaaatagc ggaggttggt ggccaccggg cgctggccga tcatgaaggt
      301 gtagtccagc gccgggccga tcgcgaagac ctggccctcg aagtcgccca gcaccgcgcc
      361 tgagccgctg tcaccggtaa tctgcttcaa ggcataacca ttcacgccga tgctcaggct
      421 gtgcgaggcg tgatacatcg cggcaccttc agcgtgaagc tccgttcccg acttgtaatc
      481 cgtatcgggg ttctcgaaat tataggtgag gcccaccgcg cccgaaagct cgaggcccgt
      541 cgtctgatcg agataggtca gggctgccgt cgtatcgatg acccagcggt tgaagccgat
      601 gttgacggga ttgccgagct cccactggcc aatcggcacg ttgatgagtg tgccaaggct
      661 gtagtgcagg tttccgtcat gccagcccaa cgtggcgccg aggacaggat cgccgaaggc
      721 ccagttgtca cgctccgcat ctgcctctcc cccgcgtccg gtaatgagtc caacttccac
      781 gcgcttgccg ccgaagggtg tcgtcagcgt gaaggcaagg ttgccgcccg gcacatccgt
      841 gcccgtcacc cacagcgccg taggaagggc gatataggca tccgcctcga cgccgcccga
      901 cagactgata cccgccgttt caaagtcgat gttggcgctg ccggtatagg catagttctg
      961 cagcaggcca tagaagcccg gcggcggaag aaagcccgcc atcgtggtct tcgagcccag
     1021 aagatagaag ctcgtgccgc cttcgacggc catggctggg ctcgagaaca cggcggtgag
     1081 tgccgcggcc tgcgcgatca agacggtacg tgttgtcata ttcatccctg cacatttcgt
     1141 tgtttcgccg tcccaaagcg aagaacgagg tcaaatcacc cgcgtcagaa ataaacgatg
     1201 acgtagccgc ctccggattt ctggtaatag gttccgccgc aataatagag gctgtagcca
     1261 taataactgc cataggcaca gccggcgggg attgccgcga tgtaagcgcc acggcggatg
     1321 gtgcggcgcg tcgtgcggcg ggccacgccc gcaacgctgc cgggggtggc gggacggccc
     1381 acacgggcct cggcatcacc gatcagcttg aagaatccga tgctggcctg ctgcccggcg
     1441 atttcgacat caccgcatat catccccagc gcgagtgcgg ccaccaggta tcgagttggc
     1501 tttcgcagtt tcattgcgcg tctccctcgc ttgcaggtgc gggcacttca tccagcccct
     1561 tcagttccgt gatggccact tccttggcgc cgtcggcagg cttcagctgg aagtcatccg
     1621 cggcgatctc cgcgccggtc ttccagcccg agaactcgat gctgtaggaa ggcgccagag
     1681 ccgtcatctt gctggtgatc gtgaagcggc agggaaatgg cttctcgccc tgcgccaccc
     1741 agatctgcca gtcgacatcg ctcgtgcgga aagcgagatg atcgcacatc tggccgcgta
     1801 tcatgccctc gcccaacgcc ttcgcgtcta tgacgttctc catcatcacc tcataagggg
     1861 cggaggacag cagatcggca gccggaagtt caaggccgaa ctcgtcgcgc agcatggcga
     1921 tcacatcatc cagcttgccc tcgagaggca gcttgccgaa gacattgagc ctcttgccat
     1981 aggcagacag cgtcttgccg tcatagacca tctcgacatc tgcgattccg ccggtccgcg
     2041 tcatgcggat cttgtccggc cggctgaaag agaggttgcc cgaactcgcg agcccgacct
     2101 tcatcatttc ggccgtcacg atctcgagcg tggcatcata gccgacggac aaggattgct
     2161 gcgccgtgag gaagtccgac atggccttga acaccgcctt ggcctcggac tgctccgccg
     2221 atgccgggga gacggcaaag gcaagaccgg ccaccacggc cgcaaatagc ttgagccgat
     2281 aaatggtcac ccgcacctcc tgttacctga agaacgccat gggccggaac ctccgctggc
     2341 agcagcgata agcggaccct ctaggactat cgtaacatga atatgagaga agcgcttatc
     2401 aaattgagcc acgcgttctg tctggagatg cgctcatgcc cgggcagcac ggactacatt
     2461 tccgttggct gggccccttc ccgggaaagc ctccagtcct gtggcgagca tcccgcccag
     2521 ccgcggaatg cgcgcgagaa agcgctgggc tcagcatatc cgagtgcgag actgatgtcg
     2581 gaaagcggcg catgaccggc agccagcagc cgccgggccc gggcaaagcg gatctcgtcc
     2641 accagctgtg agaacgaaac gccatggccc gataaccggc ggtcgagggt gcggcgtgac
     2701 aagtccatcg ccgcggcaat tgcccgcgcg gttacgggct cacgcccgat ccgcatcgcg
     2761 atctggtgcc gcacatgatc ggtgaagtcc caggctgaag gcggaagcga gccgcctcct
     2821 ctggcgccca actccggcgt cacgggctgg tccagccagt gccgggtgaa caccagggca
     2881 tccatcgcac tctcgaaccg gaccggcgcg ccgaagaatg tctcataagg tgtcacgttc
     2941 ttcggcctcg ccatcgaaag acgcacttcg gcagggcacc aatcgtcacc gcagaacctg
     3001 cgcatgaccc ggaatgcgtt tccgatggcg gtgaccagca cgatatcctc gccttcgaaa
     3061 tccgtctcgc gcaattcata ggaaaggacg gcatcgctcc cctgctcgga gagcgacagg
     3121 aatgcgaatt cattatgatg cttcagaagg cgcgcaatgt tgagcaacgc cgtccggaca
     3181 tccggcccct cctcggcaag tgtcaggatc aaccccagcg agtgaggccc ataggacttt
     3241 gcgatcaaca agccgagatc gggccgctcg atcagccgcg caatcccgga agcgaatttg
     3301 ccgagcttca cgatggagac ccggttttca gggctggcga acaggcgttc gtcgaggtca
     3361 gctgccgcaa gagcaggagc gggatcccgg ccaaagctcc tcgccagcgc aggcaggcaa
     3421 gttgccggtc caacgcgcag ggtaaattct ctcgtgtcaa acgatggcaa gacgaaacag
     3481 tgctccgctc atagtggaag gcgcttgcgg ctgccaacca tatcgcactt ccaaaaggaa
     3541 caaaatctga ccgatctgac tcggccgctg tgaaggctgg cgcaaaccgc tgctgtaaag
     3601 gaacccgaac gcttcaggtt caatccgctc accaaatgcc gggactaaac atctaattgg
     3661 tctgcgcgcg ttgggcatct tctccaagcc gatcccggct ccgcactcgg tccggacggc
     3721 cgcagcgcct gcggcatcaa agagttggat tatcgggcca ctcgcacacc agagcaaagg
     3781 tacaaagtac ggcaggaaga tcaactaaaa ttcatttaat ttcagatgat tatcgccgcc
     3841 gtacttttcc cgcgcagtca ggagctttgg agaataaggg gccgagagag acgaaatccg
     3901 gcccccttgc ttgtcatgga tgtctcccgg tggacaatag ggcatatgat gatgcacgtg
     3961 gacggacagc gcgccgccat atcggcttca gcgcctcgag caccagaagc acggccaggc
     4021 cagccagtgg cggaacagca agaagcagtg gctccagcac accgaaacca aagagttggc
     4081 gggcaggttg ccagaacagg gtggcagcca gaaggcagat cacaatcggc actaccaccg
     4141 caagcgccct gttgggacgg cggattgccg tgatgatgga ggacgactgc gagcggtcca
     4201 cgaggatcaa cgccatgatg cagagcacca gcgaggcgaa ggtcagcccg caaacctgat
     4261 cggcctccag cccatactgc ggggcgagga gaaaaaccat gccggttacc cccagaacaa
     4321 acaacccttg aacgaggctc cacaggatcg tcgggacgga gaacagtggt tcgtccggag
     4381 ggcgtggcgg gcgt
//
LOCUS       DBNGVR010000015         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3738562, whole genome shotgun sequence.
ACCESSION   DBNGVR010000015 DBNGVR010000000
VERSION     DBNGVR010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3738562"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16A0_00330"
     CDS             <1..231
                     /locus_tag="AC16A0_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3103799.1"
                     /translation="GGANLMGGAGTAFGAVIGSAFLEVIRNALLMAGIDSNWQGAFVG
                     AFIILAVLLGMQSGGKSLIAATVERLFPKKHR"
     gene            283..1287
                     /locus_tag="AC16A0_00335"
     CDS             283..1287
                     /locus_tag="AC16A0_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HIR3103800.1"
                     /translation="MKKLLMSTILAVGALTIAATGAQAKFVFALVPKNMNNPFFDQAR
                     DGCKKAEAESNGAFECMYIGPGEHGGGDEQVTIVQDLVAKKVDGIAVSPSNAAAMAVA
                     LQAAKEAGIPVLTWDSDLLPENKDLRVAYIGTHNYEIGTNLAKLAKAIKPNGGTVCIQ
                     SGGAAAANHNERMQGIRDELSGAKSAASPGDRLTGQGGWTEAEGCPLYTDDDFPRSVQ
                     QFEDFMAANPTLDAFIPTGGFPQFIPDANRAAVGKYKDKIAAKELALVVADTLPVQIE
                     QMKEGLSLGQVGQRPFEMGYKTMMALKDMKEGKAAPADPTYTGLDVCTPETADTCIAK
                     "
     gene            complement(1349..2128)
                     /locus_tag="AC16A0_00340"
     CDS             complement(1349..2128)
                     /locus_tag="AC16A0_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3103801.1"
                     /translation="MALALQAAAAAPLVLRQRDGRRPRLLLAEDCDPVRIVTAAMLKG
                     MGCDVDAVVHGEEAVRSACAQSFDVIVLDIEMPIMDGITAARSIRNLGGAVSATPIMA
                     LSAFLADAMQQASWSDTFDIALPKPANRNELHTAVQAALDWRPRQAADGEAPLINLQQ
                     MSDLSAGLSAGVFRELLNMACRDIEVCANQLTHLQKSAIRGADHVYVQKLMALGQSFA
                     APRLSEAAERVASAGSLASRPQMISGLIVTAAATAAALRAY"
     gene            complement(2241..>2733)
                     /gene="upp"
                     /locus_tag="AC16A0_00345"
     CDS             complement(2241..>2733)
                     /gene="upp"
                     /locus_tag="AC16A0_00345"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3103802.1"
                     /translation="DLPITTQTIETPLAEMEAPILKGKKLVIVSILRAGNGLLEGMLD
                     IMPSARIGHIGLNRDPETLQPVEYYLKVPEDISERPVIVVDPMLATGNSVSYAIKRLM
                     EKGAKDIRLVCLLSAPEGIRQVCGAHPDVKVYTAAVDSHLNDHGYIVPGLGDAGDRMF
                     GTK"
BASE COUNT          526 a          855 c          891 g          461 t
ORIGIN      
        1 ggcggcgcca atctcatggg cggcgccggc acggccttcg gcgcggtgat cggctcggcc
       61 ttcctggagg tcatccgcaa tgcgctgctg atggctggca tcgattcgaa ttggcaaggt
      121 gccttcgtcg gcgccttcat cattctcgcg gtacttctcg gcatgcaatc cggcggcaaa
      181 tccctgattg ccgccacggt ggagcgcctg tttccgaaga agcatcgcta gaagccggtt
      241 tcactgccgg acacagtgaa ggtcatagag gaggaagacc caatgaagaa actgctcatg
      301 agcacgatcc ttgccgtcgg ggccctgacg atcgcggcca ccggcgccca ggcgaagttc
      361 gtcttcgcac ttgtcccgaa gaacatgaac aacccgttct tcgaccaggc tcgcgacggc
      421 tgcaagaagg ccgaagccga atccaacggc gctttcgagt gcatgtacat cggccccgga
      481 gagcatggcg gcggtgacga acaggtcacg atcgtccagg atctcgtggc aaagaaggtg
      541 gatggcatcg ccgtgtcgcc gtcgaatgcg gccgccatgg cggttgcgct gcaggccgcc
      601 aaggaagcgg gcatcccggt tctgacctgg gactccgacc tgctgcccga gaacaaggat
      661 ctccgcgtgg cttacatcgg cacccacaac tacgagatcg gcaccaacct cgccaagctc
      721 gccaaggcga tcaagcccaa tggcggaacc gtctgcatcc agtccggcgg cgccgcggcc
      781 gccaaccaca acgagcgcat gcagggcatc cgcgacgagc tgtcgggcgc caagtccgcc
      841 gcctcgccgg gcgatcgcct gaccggccag ggtggttgga ccgaggcaga aggctgcccg
      901 ctctacacgg atgacgactt cccgagatcc gtgcagcagt tcgaggactt catggccgcc
      961 aaccccaccc tcgacgcctt catcccgacc ggcggcttcc cgcagttcat tccggacgcg
     1021 aaccgcgcag cggtgggcaa gtacaaggac aagatcgcag ccaaggaact ggctctggtc
     1081 gtggccgaca cgctgcccgt ccagatcgag cagatgaagg aaggcctgtc gcttggccag
     1141 gtcggccagc gtcccttcga gatgggctac aagacgatga tggcgctgaa ggacatgaag
     1201 gaaggcaagg cggcgccggc cgatccgacc tacacgggcc tcgacgtctg cacgcccgaa
     1261 acggccgata cctgcatcgc gaagtagtcg acagaaggat cgaatcgaga ggggcggagg
     1321 attctccgcc ctttttcgat ggaagccgtc agtacgcccg cagggccgcc gcggtggcgg
     1381 ccgcggtgac gatcagcccg gaaatcatct gcggacggct ggcgagactg ccggcggatg
     1441 ccacgcgctc ggccgcctct gacaggcgcg gtgcggcgaa gctctggccc agcgccatca
     1501 gcttctgcac atagacatga tcggcgccgc ggatggccga cttctggaga tgcgtcagct
     1561 ggttggcgca gacctcgatg tcgcgacagg ccatgttgag gagttcgcga aagacgccgg
     1621 ccgacaggcc cgcagacagg tccgacatct gctggagatt gatcaggggc gcctcgccgt
     1681 ctgcggcctg ccgcgggcgc cagtcaagtg ccgcctgaac ggcagtgtgc agttcattgc
     1741 ggttggcagg cttcggcagg gcgatgtcga aggtgtcgct ccagcttgcc tgctgcatgg
     1801 catcggccag aaaggccgac agcgccatga tcggcgtcgc gctgacggcg ccgccaagat
     1861 tgcggatgga gcgtgccgcc gtgatgccat ccatgatcgg catctcgatg tcgagcacga
     1921 tgacgtcgaa gctctgggca caggcggagc ggacggcttc ctcgccatgg accacggcat
     1981 ccacgtcgca tcccatgccc ttgagcatgg cagccgtcac gatgcggaca gggtcgcaat
     2041 cttcggcaag caagaggcgc ggacggcgac cgtcgcgctg ccgaagcacc agaggtgcag
     2101 ctgcggcggc ctgcagtgca agagccattc caattcccaa tccaaaaacg tcccggtcca
     2161 gaccgtagcg gcgggatggt ttgcaagtgg ttgccgaaac cactccaatt ggaagatggc
     2221 taatgcgagg ttaaggcttt ctacttggta ccgaacatgc ggtcgccggc gtcacccaga
     2281 cctggaacga tgtatccgtg atcattgaga tggctgtcga cggcggccgt atagaccttc
     2341 acatcgggat gcgcgccgca gacctggcgg attccttccg gtgccgacag gaggcagacg
     2401 agacggatat ccttggcccc cttttccatc aggcgcttga tggcatagga gaccgaattg
     2461 ccggtcgcca gcatgggatc gaccacgatc accgggcgct cggaaatgtc ctcgggcacc
     2521 ttcaggtagt attcgacggg ctgcagcgtc tcgggatcgc ggttgagccc gatatggccg
     2581 atgcgggccg aaggcatgat gtccagcatg ccttcgagca ggccgttgcc ggctcgcagg
     2641 atcgacacga tgaccagttt cttgcccttc aggatgggcg cttccatctc ggccaggggc
     2701 gtctcgatgg tctgcgttgt gatcggcaga tcg
//
LOCUS       DBNGVR010000016         6229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3738701, whole genome shotgun sequence.
ACCESSION   DBNGVR010000016 DBNGVR010000000
VERSION     DBNGVR010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3738701"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..910
                     /locus_tag="AC16A0_00350"
     CDS             137..910
                     /locus_tag="AC16A0_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025389.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR3103803.1"
                     /translation="MVTQLINYALPLFTGLFLAIVLATELGRRIGSWRQQRVGGPLPG
                     TAAVDAAIFALFGLLLAFAFSSAAARFDHRRDMIVAEANDIGTAYLRIDLAPQAAQPA
                     LRDAFRRYVDSRVNAYRKGIDFEDFKAGLEASEEIQKEIWLLAIAAGKMPDAPPNLNM
                     LLLPALNQMIDITTTRAMATVSHPPAIIYVLLFALALICATIAGHGMAAARSRNWFHM
                     IGFAAILTITLYVIVDLEFPRLGFIQVGDFDKLLLRSVD"
     gene            1058..1348
                     /locus_tag="AC16A0_00355"
     CDS             1058..1348
                     /locus_tag="AC16A0_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BufA1 family periplasmic bufferin-type
                     metallophore"
                     /protein_id="HIR3103804.1"
                     /translation="MNTSRRNTMLAIAGAMTAAIAGTAMTAAAQAADMEKCYGIALKG
                     QNDCAAGEGTTCAGTSTTDYQGNAWKLVPKGTCATTETPLGPGSLEPIKRPA"
     gene            1389..2243
                     /gene="bufB"
                     /locus_tag="AC16A0_00360"
     CDS             1389..2243
                     /gene="bufB"
                     /locus_tag="AC16A0_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MNIO family bufferin maturase"
                     /protein_id="HIR3103805.1"
                     /translation="MSTPAPDIPARGGVGLKAEHYKSVLAESPDLGFFEIHAENYMGA
                     GGPPHRYLSAIRDLYPLSLHGVGLSIGGPGRLDADHLMRLKALIDRYQPGLFSEHLAW
                     STHETGYLSDLLPLPYTAATLQTVCDHIDETQSVLGRQMLLENPSSYLRFAESSFDEA
                     GFIAEVVRRTGCGLLLDVNNVFVSARNLATVAMDYLAAYPLSHVQEIHLAGHDVIVEN
                     GGAVLIDSHAAPVVDDVWQLYAAVIGKTGPLPTLIERDANVPPLAELVAEAGQAERIM
                     RSSSRAAA"
     gene            2240..2926
                     /locus_tag="AC16A0_00365"
     CDS             2240..2926
                     /locus_tag="AC16A0_00365"
                     /inference="COORDINATES: protein motif:HMM:NF021364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfC/BufC family peptide modification chaperone"
                     /protein_id="HIR3103806.1"
                     /translation="MIGQCEFAAAIGDWSLVPADPRFRIYRNNVTSALINALRVRYPV
                     TERILGREAFAACAGSYAETQRPASPVLIDYGASFPDHLAAVSHDARAADLARLESLW
                     WQAYHAADASPLPPEKLSEIPPESWGRARFAFLPSLGLMSSPHAVGSLWQGQDGAFGP
                     QNVVVARPEADVTLHLMGPAPFALLVRLCHGNTLAEAVETTSGTYADFNLTQSLQGLL
                     ALRIITGVST"
     gene            2923..3357
                     /locus_tag="AC16A0_00370"
     CDS             2923..3357
                     /locus_tag="AC16A0_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004127233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3103807.1"
                     /translation="MIRLLYGLDGLFSRIPQSFIALLGRLAIGLVFWNSGRTKVQGWN
                     IFDVSETTLYLFQNDYKVPLLPPEVAALMTQVAESTLPILLFIGLATRYAAVGLLVMT
                     LVIEVFVYPGAYVVHGTWATILLMLIKWGPGVFSVDHLISRR"
     gene            complement(3369..4154)
                     /locus_tag="AC16A0_00375"
     CDS             complement(3369..4154)
                     /locus_tag="AC16A0_00375"
                     /inference="COORDINATES: protein motif:HMM:NF021364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfC/BufC family peptide modification chaperone"
                     /protein_id="HIR3103808.1"
                     /translation="MSSLSNLQSRLQADILGGSLDAEDLIAPAPRGTRASRLDVYRRA
                     YVLRLTEFLSNDYEKLRIYLGETRFNRMARDYAAAHPSDTPNARWFSRHLPAFLRQSK
                     AFLPHPEVGELAMLEQALNDVFDGPEAAPTTLADLAAIDPTAFGDVRFALVPACMTVE
                     ARSNATSIWSAIRCGETPPAAERLDMPQHILVWRQAGSSRFRILGEEEFMALSCVAEG
                     LDFATICEMLAAYDDPETAALRAANYLRGWIEAEILVRTPAAS"
     gene            complement(4151..4999)
                     /gene="bufB"
                     /locus_tag="AC16A0_00380"
     CDS             complement(4151..4999)
                     /gene="bufB"
                     /locus_tag="AC16A0_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MNIO family bufferin maturase"
                     /protein_id="HIR3103809.1"
                     /translation="MTHHTLPYLGFGLGLRPQHYREILETAPHVDWFEVISENYMVPG
                     GQPLRMLDRIAERYPLVMHGVSLSIASTADLDMDYLAQLKELARRIEPRWISDHLCWT
                     GVHGVNLHDLLPVPYTEEALAHVSDRIARVQDYLGRRLVIENVSSYITYECSEMSEWD
                     FVAELARRADCLLLLDVNNVFVSGMNHDFDQRAFIESIPVDRVVQFHLAGHSEGETCL
                     IDTHDQPVCEEVWTLYGKALERFGAVSTMIERDDNIPPLADLVAELDTARAIAAQALR
                     TLERVS"
     gene            complement(5049..5303)
                     /gene="bufA2"
                     /locus_tag="AC16A0_00385"
     CDS             complement(5049..5303)
                     /gene="bufA2"
                     /locus_tag="AC16A0_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BufA2 family periplasmic bufferin-type
                     metallophore"
                     /protein_id="HIR3103810.1"
                     /translation="MKHIGKSGALLAAAAFSFAVSGATIAPVQADEAKVHCVGVNSCK
                     GQSECKTASSSCKGQNGCKGQGFLSMTKADCETAGGKVES"
     gene            5519..6145
                     /locus_tag="AC16A0_00390"
     CDS             5519..6145
                     /locus_tag="AC16A0_00390"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3103811.1"
                     /translation="MKSRLLAGCASLMILLSANGAAWADAYQDGMLAFKNEDYGTALT
                     LLMPFAKQGDAEAAYLIGEMYGPRSWGQKGENRNGVEQDNAQAILWWGEAAKAGLAEA
                     QLKLGWWLLKGKDVVKQDEAAGMNWLLMAANQGEPQAQYEVGIGYWKGRGVKPDPIAA
                     YMWLELASTEPGFDNAVGYLEELAKTMTPEQVTQARQMVQAFAPKRSQ"
BASE COUNT         1104 a         2009 c         1920 g         1196 t
ORIGIN      
        1 tcatgattgc tccgggcagg tggtcatgaa acatagtagc gctccctggc cgcgcttggc
       61 gaggggggcc cggacatcct tctggtgcat cgcatcatgg aatcgattat gaatcctggg
      121 cgactggagc agacccgtgg ttacgcagct aatcaactat gccttgccgc tcttcacggg
      181 actgttcctg gccattgtcc ttgcaaccga actggggcgg cggatcggca gctggcgtca
      241 gcagcgcgtg ggcggaccct tgcctggtac ggctgcggta gatgccgcca tttttgcgct
      301 cttcggtttg ctcctggcct ttgccttctc cagcgctgcg gcgcgcttcg accatcgccg
      361 ggacatgatc gtcgcggagg ccaatgacat cggcaccgct taccttcgca tcgacttggc
      421 tccccaggca gcgcagcctg ctctgcggga cgccttccgg cgctatgtcg actcgcgcgt
      481 gaacgcttac cgcaagggca ttgacttcga ggacttcaag gccggactgg aggcatcgga
      541 agagattcag aaggaaatct ggttgctcgc gattgcggca ggaaaaatgc cggatgcgcc
      601 cccgaacctc aacatgctcc tgctgccggc cctgaaccag atgatcgaca tcaccacgac
      661 gcgggcaatg gcgacggtct cgcatccgcc cgccatcatc tatgtgctgc tcttcgcctt
      721 ggcgctgatc tgcgcaacca tcgcaggtca cggcatggcc gcagcacggt cgcgaaactg
      781 gtttcacatg attggcttcg cggcaatcct gaccatcacg ctttatgtga tcgtcgacct
      841 cgagtttccg cgcctcgggt tcattcaggt cggggacttc gacaagctac tgttgcgatc
      901 ggtcgactga catgctgcag ttgcgaatgc cttttgtgta acgagaactg cacatattgg
      961 tgatatcgac gaagtgcttt gtttacagtc agttgatttc cccttgacct tcgactctgc
     1021 aatgctacgc atgctgaatt caacacagga gacgcccatg aacacctctc gccgcaacac
     1081 catgctcgcc atcgccggag ccatgacagc cgccattgcc gggaccgcca tgaccgcggc
     1141 cgcccaggcc gccgacatgg aaaagtgcta cggaattgcg ctcaagggcc agaatgactg
     1201 cgcggccggc gaaggcacga cctgtgccgg aacctcgacg accgactatc agggcaatgc
     1261 ctggaagctg gttcccaagg gcacctgcgc gacgaccgag acgccgctcg gccccggctc
     1321 gctcgaaccc atcaagcggc cggcctgatc gccacctggg gcgcgggtca ttccgcgccc
     1381 cgaattccat gtcaacacca gctccagaca tccctgcgcg cggcggcgtc ggactcaagg
     1441 ccgaacacta caagtcggtg ctggctgaat cgccggatct cggcttcttc gagatccatg
     1501 ccgaaaacta catgggcgcc ggcggcccgc cgcaccgcta tctttccgcc attcgcgacc
     1561 tctaccccct ttccctgcat ggcgtgggcc tgtcgatcgg gggtccgggc cggctggacg
     1621 ccgatcatct gatgcgcctc aaggccctga ttgatcgcta tcagccaggc ctcttctcgg
     1681 aacatctcgc ctggtccacc catgagaccg gctatctcag cgaccttctt ccgcttccct
     1741 acacggccgc gacgcttcag acggtctgcg atcacattga cgagacgcag tcggttctcg
     1801 gccgccagat gcttctggag aacccctcca gctacctgcg cttcgcagag agcagcttcg
     1861 acgaggccgg gttcattgcg gaagtcgtcc ggcgcactgg ctgcggcctg ctgctcgacg
     1921 tcaacaatgt cttcgtctcg gcccgcaatc tcgcgaccgt tgcgatggat tatctcgcag
     1981 cctacccctt gtcgcatgtt caggaaatcc atcttgccgg acatgacgtc atcgtggaga
     2041 acggtggtgc ggtgctgatc gactcgcatg cggcgccggt ggtggacgac gtgtggcaac
     2101 tctacgccgc cgtcatcggc aagaccggtc cgctcccgac actgatcgaa cgcgacgcca
     2161 atgtcccgcc gcttgccgag ctggtcgccg aggccggtca ggccgagcgc atcatgcgca
     2221 gctcctcgcg ggccgcagca tgattggcca gtgcgagttc gcggcagcca tcggcgactg
     2281 gtctctcgtg ccggccgatc cgcgcttccg catctaccgc aacaacgtga cgtcggcgct
     2341 gatcaatgcg ttgcgcgtgc gctatccggt taccgagcgg attctggggc gcgaggcctt
     2401 tgccgcctgt gcaggatcct atgccgagac acaacgcccc gcatcgccgg tgcttatcga
     2461 ttatggcgcc agtttccccg accatctcgc cgcggtgtct catgacgccc gtgcggccga
     2521 tctcgcccgg ctcgaaagcc tgtggtggca ggcctatcat gcggccgatg cgagcccgct
     2581 gccgccggag aagctctccg agatcccgcc cgagagctgg ggccgggcgc ggtttgcctt
     2641 cctcccctcc cttggcctca tgtcttcccc tcatgccgtc ggctcactct ggcagggtca
     2701 ggacggagcg ttcggcccac agaacgtcgt cgtcgccagg cccgaagcgg acgtgacgct
     2761 gcacctgatg gggcctgcgc ccttcgcgct tcttgtcaga ctgtgccatg gcaacacgct
     2821 ggccgaggcg gtcgagacca cctccggcac atatgcagat ttcaacctca cccaatcgct
     2881 tcagggactc ttggccctgc gcatcatcac gggagtctcc acatgatccg cctgctctac
     2941 ggcctcgatg gcctcttcag ccgtattccc cagtccttca tcgccctgct cgggcggctt
     3001 gcgatcggac tggtgttctg gaattcgggc cgcaccaagg tgcagggctg gaacatcttc
     3061 gatgtcagcg agaccacgct ctacctcttc cagaacgact acaaggtgcc cctgcttccg
     3121 cccgaagtgg cggccctgat gacgcaagtg gccgaaagca cgctgccgat ccttctcttc
     3181 atcggattgg ccacgcgcta tgccgccgtc ggactactgg tgatgacgct ggtcatcgag
     3241 gtcttcgtct atcccggcgc ctatgtggtt cacgggacat gggccacgat cctgctgatg
     3301 ttgatcaagt gggggcccgg cgtgttttcc gtcgatcacc tgatcagccg ccgctgatgc
     3361 aatgaccgtc aacttgcggc gggggtcctg accaggatct ccgcctcgat ccatccccga
     3421 aggtagttcg cggccctcag ggcagcggtt tcgggatcat catatgcggc gagcatttcg
     3481 cagatggtgg caaagtcgag accttcggcc acacagctga gcgccatgaa ttcttcttcg
     3541 cccaggatgc ggaagcgaga gctgcccgcc tgacgccata cgagaatgtg ctgcggcatg
     3601 tcgagccgtt ctgccgccgg tggcgtctcg ccgcaacgga tggctgacca gatactggtg
     3661 gcgttgctgc gggcctcgac cgtcatgcag gccgggacga gcgcaaagcg cacgtcaccg
     3721 aaggccgtgg gatcgatggc cgcgagatcc gccagtgttg tcggcgccgc ttccgggccg
     3781 tcgaagacat cattcaacgc ctgttccagc atggccagtt cgcccacttc cggatgtggc
     3841 aggaatgcct tcgactgccg caggaaggcc ggcagatggc gcgagaacca gcgggcattg
     3901 ggcgtatcgg agggatgagc tgcagcatag tcccgcgcca tccggttgaa gcgcgtctcg
     3961 cccagataga ttcgcagctt ctcgtagtcg ttgctgagga attcggtgag ccgcagcaca
     4021 taggcacggc gatagacatc caggcggctt gcccgggtgc cgcgcggcgc cggtgcaatc
     4081 aggtcttcag cgtccaggct cccgccgaga atgtcagcct ggagccttga ctggaggttc
     4141 gacaggctgc tcatgacacg cgctccagcg tgcggagcgc ctgagcggca atggctcttg
     4201 ccgtgtcgag ttcggccacc agatcggcaa gcggcggaat gttgtcgtcg cgctcgatca
     4261 tggtcgatac ggccccgaaa cgctcaagag ccttgccata gagcgtccag acttcctcgc
     4321 agaccggttg atcatgcgtg tcgatcaggc aggtctcgcc ctcgctgtgg cccgccagat
     4381 ggaactggac gacgcggtcg accggaatgg attcgatgaa ggcgcgttga tcgaagtcgt
     4441 gattcatgcc cgacacgaag acattgttga cgtcgagcag gagcaggcag tccgcccgtc
     4501 gtgcgagctc ggcgacaaaa tcccattcgc tcatttcaga gcactcatat gtgatgtagc
     4561 tcgagacgtt ctcgatgacg aggcggcggc caaggtagtc ctgcacccgc gcgatgcggt
     4621 ccgatacatg ggccagcgcc tcctccgtat agggcaccgg caggagatcg tgaaggttca
     4681 cgccatgaac gccggtccag cagagatggt cggatatcca gcgcggttcg atgcggcgcg
     4741 cgagttcctt caactgtgcc agataatcca tgtcgaggtc cgccgtcgac gcgatcgaca
     4801 gcgacacgcc atgcatcacc agcggatagc gctcggcgat gcggtcaagc atccgcaggg
     4861 gctgaccgcc cggcaccatg tagttctccg agatcacctc gaaccagtcc acatgcgggg
     4921 cggtttccag aatctcgcgg taatgctgcg gccggaggcc cagcccgaag ccgaggtatg
     4981 gcagagtgtg atgggtcatc cgcgagtggc gcgccgcggg tccggttccc gcggtgcgcc
     5041 ctgttcgatc agctctcgac ctttccgccc gcggtctcgc agtcggcctt ggtcatgctg
     5101 aggaagccct ggcccttgca gccgttctgg cccttgcacg acgagctggc ggtcttgcac
     5161 tcagactggc ccttgcagga gttgacgccg acgcaatgca ccttcgcctc gtcagcctgt
     5221 accggagcga tggtcgctcc gcttacggcg aacgagaagg cggcggcggc gagcagggct
     5281 cccgactttc caatatgctt catctgaggt gtctcccttg gtcgttggtt ggtttgcgaa
     5341 caccgtgtcc gcaacaccga ccttggcgaa agccttgatc actcgccagt cacgagcgcg
     5401 ttttggccct cacaacccgt tgaagcggcg tcattcgagc cgtgaacggc cgtgtttgca
     5461 aaatggccgg caaagggcta ggcagacatc gaagtaagct agttgcgagg acccgacgat
     5521 gaaatcacgc ctcctggccg gttgcgccag cctgatgatc ctgctttccg ccaatggcgc
     5581 ggcttgggcc gacgcctatc aggacgggat gctggccttc aagaacgagg actatggcac
     5641 cgcgctcacc ctgctcatgc cctttgccaa gcagggcgac gccgaagcag cctacctcat
     5701 cggcgagatg tacgggccgc gcagctgggg ccagaagggc gagaaccgca acggtgtgga
     5761 acaggacaat gcccaggcca ttctctggtg gggcgaggcc gccaaggccg ggcttgccga
     5821 ggcgcagctc aagctgggct ggtggctgct caagggcaag gacgtggtca agcaggatga
     5881 ggctgccggc atgaactggc ttctcatggc agccaatcag ggcgagcccc aggcacagta
     5941 cgaggtgggc atcggttatt ggaagggccg cggcgtgaag ccggatccga tcgccgccta
     6001 catgtggctg gaactggcct cgaccgagcc cggcttcgac aatgcggtcg gttatctcga
     6061 ggaactcgca aagaccatga cgcccgagca ggtgacgcaa gccaggcaga tggttcaggc
     6121 cttcgcgccg aagcggtcgc agtaggctgg gcggtcgcgc ccgtcacccg cccagcgcga
     6181 tggtgattgc cacaaggacg actccgagga ccaccatggc ggcggccat
//
LOCUS       DBNGVR010000017         2697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_399589, whole genome shotgun sequence.
ACCESSION   DBNGVR010000017 DBNGVR010000000
VERSION     DBNGVR010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_399589"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2163
                     /locus_tag="AC16A0_00395"
     CDS             <1..2163
                     /locus_tag="AC16A0_00395"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR3103812.1"
                     /translation="VNWDPKLLTAISDLEVVQKEVKGNLWHLRYPVDGTDEVIVVATT
                     RPETMLGDTAVAVHPENGKLKHLIGKFARLPLVGRLIPIIGDDYADPEKGTGAVKITP
                     AHDFNDFEVGKRHNLPAINILDQFGKLSLQEPDFVAGVPASDRLMDVLTLHGTDRFEA
                     RKIIVAKLDDLGLLEKIEPHVHQVPHGDRSDVVIEPWLTEQWYVNAAELAKPAITAVE
                     TGDTVFVPKNWEKTYFEWMRNIQPWCISRQLWWGHQIPAWYGPDGSVFVEETEEAARA
                     AAKARFGKDVDLARDEDVLDTWFSSALWPFSTLGWPDQTPELKRHYKTDVLVTGFDII
                     FFWVARMMMMGLHFMKEVPFHTVYIHALVRDEKGQKMSKSKGNVIDPIDLIDAYGADA
                     LRFTLAAMAAQGRDIKLSKQRVEGYRNFGTKLWNAARFAEMNGVAHDPNFDPIAARVT
                     VNRWIAGETARTRNAVTRAIEEHKYNEAAGALYQFVWNVFCDWYLELIKPILTGTDEA
                     AKAETRASTAWVLDQILLLLHPFMPFVTEELWQQTGARKTWLIEADWPQYKGLGDAAA
                     DAEIEWVIRFISEVRSVRAEMNVPAGAKIACVMVGAGGETRRRASQWEAEIMRLARLS
                     AIGFEDAVPRASAQIVLGEATVALPLEGVIDIAAEKARLAKELEKIARDTAGIESRLG
                     NPGFVAKAPPEVLEESRERKAELEARRSKVEEALKRLG"
     gene            complement(2276..>2697)
                     /locus_tag="AC16A0_00400"
     CDS             complement(2276..>2697)
                     /locus_tag="AC16A0_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR3103813.1"
                     /translation="VSVIPAKAGIHFSTSAREFTYHVLASHNPAFMVSPLKEEKQPAV
                     YILASRRHGTLYIGVTSNLCNRVSSHKQGLAEGFTKRYGVKMLFWFQEFASMEDAIRR
                     EKQLKEWRRAWKIELIEKLNPTWLDHFGEVCGLTDPD"
BASE COUNT          523 a          833 c          859 g          482 t
ORIGIN      
        1 gtgaactggg acccgaagct gctgaccgcc atctccgacc tggaagtggt gcagaaggag
       61 gtgaagggga atctctggca tctgcgttat ccggtcgacg gcacggatga ggtcatcgtg
      121 gtggccacga cccggccgga aaccatgctg ggcgacacgg ccgttgccgt tcaccccgaa
      181 aacgggaagc tcaagcatct gatcggcaag ttcgcacggc tgccgctcgt tggccggctc
      241 attccgatta ttggtgacga ctacgccgat ccggagaagg gcaccggcgc ggtgaaaatc
      301 acaccggcgc atgacttcaa cgacttcgag gtgggcaagc ggcataatct gccggccatc
      361 aacatcctcg accagttcgg caagctttca ctgcaagagc ctgatttcgt tgccggagtt
      421 ccggcgagcg acaggctgat ggacgtcctc accctgcacg gcaccgaccg cttcgaggcg
      481 cgcaagatca tcgtcgccaa acttgacgac ctgggcctgc tggagaaaat cgagccgcat
      541 gtccaccagg ttccgcatgg cgaccgctcc gacgtggtga tcgagccctg gctgaccgag
      601 cagtggtacg tgaacgccgc cgaactggcc aagccagcca tcaccgcagt ggaaacaggc
      661 gacaccgtct tcgttccgaa gaactgggaa aagacctatt tcgaatggat gcgcaacatc
      721 cagccctggt gcatctcgcg ccagctgtgg tgggggcatc agattcccgc gtggtatggg
      781 cccgatgggt ctgtcttcgt cgaagagacc gaggaggcgg cacgtgctgc cgccaaggcc
      841 cgctttggca aggacgtgga cctcgcgcgc gacgaagacg tcctcgacac atggttctcc
      901 tcggcgctct ggcccttctc cacgctgggc tggcccgatc agacgccgga actcaagcgg
      961 cactacaaga ccgacgtgct ggtcaccggc ttcgacatca tcttcttctg ggtggcccgc
     1021 atgatgatga tggggctgca cttcatgaag gaggtgccgt tccacaccgt ctacatccat
     1081 gccctggtcc gcgacgagaa gggccagaag atgtcgaagt cgaagggcaa cgtcatcgat
     1141 ccgatcgacc tcatcgatgc ctatggcgcc gacgcgctgc gtttcacgct ggccgcaatg
     1201 gccgcccagg gccgcgacat caagctgtcc aagcagcgcg tggaaggtta tcgcaacttc
     1261 ggcaccaagc tgtggaatgc cgcgcgtttc gccgaaatga acggtgtggc gcatgatccg
     1321 aacttcgatc cgatcgcggc gcgggtcacc gtcaaccgct ggattgccgg cgagacggcg
     1381 cgcacccgca atgccgtcac ccgtgccatc gaggaacaca agtacaacga ggctgcgggc
     1441 gcgctctacc agttcgtctg gaacgtcttc tgcgactggt atctggagct gatcaagccg
     1501 atcctcaccg gcacggacga ggccgccaag gccgagaccc gcgccagcac cgcctgggtg
     1561 ctggatcaga tcctgcttct cctgcatccc ttcatgccct tcgtgacgga ggagctctgg
     1621 cagcagacgg gagcacgcaa gacctggctg atcgaggcgg actggccgca gtacaagggt
     1681 ctgggcgacg ccgctgccga tgccgagatc gagtgggtga tccgcttcat ctccgaagtg
     1741 cgctcggtgc gcgccgaaat gaacgttccg gccggcgcca agatcgcctg cgtcatggtg
     1801 ggtgccggag gcgagacgcg ccgccgcgcc agtcagtggg aggcggagat catgcgcctg
     1861 gcgcggctgt cggccatcgg tttcgaggat gccgtgcccc gggcctccgc ccagatcgtg
     1921 ctgggcgagg caaccgtggc gctgccgctg gaaggcgtga tcgacatcgc cgccgagaag
     1981 gcgcgcctcg cgaaggaact ggagaagatc gccagggaca cggccggcat cgagtcccgc
     2041 ctcggtaatc ccggcttcgt cgccaaggca ccgcccgaag tgctggagga gagccgcgaa
     2101 cgcaaggcgg agctggaggc ccgcaggtcg aaggtggagg aggcgttgaa gcggctgggg
     2161 taggcatccc gcgtcgcgca cttcagttgg atcaactgtt ctctttggac ggtgagtggc
     2221 aggaggcctc cctatcctcc gtcaccccgg cgaacgccgg ggtccattga gccggtcagt
     2281 ccgggtccgt cagcccgcag acttcgccaa agtgatccaa ccacgtcgga ttgagtttct
     2341 cgatcagctc gatcttccac gcgcgtctcc actccttgag ctgcttctcg cggcggatgg
     2401 catcctccat ggatgcaaac tcctggaacc agaacagcat ctttaccccg taccgcttgg
     2461 tgaacccctc ggccaatccc tgtttgtggg atgacacccg gttgcaaagg tttgaagtga
     2521 cgccgatgta gagggttcca tgccgacgag aggcgagaat gtacaccgcg ggctgcttct
     2581 cttctttcag cgggctgacc atgaatgctg ggttatgtga tgctaacaca tgataagtga
     2641 attctcgcgc agaagtgctg aaatggatcc cggctttcgc cgggatgacg gaaacaa
//
LOCUS       DBNGVR010000018         4864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2364359, whole genome shotgun sequence.
ACCESSION   DBNGVR010000018 DBNGVR010000000
VERSION     DBNGVR010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2364359"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="AC16A0_00405"
     CDS             complement(<1..1007)
                     /locus_tag="AC16A0_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3103814.1"
                     /translation="MTGAEIVLKALADQGVEHVFGYPGGAVLPIYDEIFQQEKVKHIL
                     VRHEQGATHMAEGYARSTGKCGVVLVTSGPGATNAVTGLTDALMDSIPMVCITGQVPT
                     HLIGNDAFQECDTVGITRPCTKHNYLVKDVNDLARILHEAFYVATHGRPGPVVVDIPK
                     DVQFATGKYVGPKNIEHKTYRPKVKPDQNAIRAAVELIATAKRPVLYTGGGVINSGPT
                     ASKLLREFVALTNIPITSTLMGLGAYPAAGKNWLGMLGMHGMYEANMSMHDCDVMINI
                     GARFDDRITGRLNAFSPGSKKIHIDIDPSSINKTVRVDIPVVGDVAHALEDMIRVWKS
                     IS"
     gene            complement(1218..1739)
                     /locus_tag="AC16A0_00410"
     CDS             complement(1218..1739)
                     /locus_tag="AC16A0_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103815.1"
                     /translation="MNTDTSTSGTDHALAPRRYTDVRAALQLSYEEEISGAAYFAHLA
                     SLFEGRPHEALMLLARIEAVTAAAIRPLVVHYGIEPQPSATLRAAGRAEAERRRGQSW
                     AMLVAEMARDFPAYVVEFDEIIALAPAEDRAGLAVLRDHEVAAVDFAQMERAGDPGSL
                     SPLHDFLARYQMP"
     gene            complement(1729..2595)
                     /gene="miaA"
                     /locus_tag="AC16A0_00415"
     CDS             complement(1729..2595)
                     /gene="miaA"
                     /locus_tag="AC16A0_00415"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3103816.1"
                     /translation="MGKTPHKRALLIAGPTASGKSALAMDVARNLNGVIINADALQVY
                     RELRILTARPLEEELAQAPHRLYGHVPGSEGYTVARWLQDVAQAIADSWREGRLPIIT
                     GGTGLYFRALEKGLSSIPPVSQEIRDHWRSYDGDLHGELAARDPEMARRLRPQDRQRI
                     VRALEVVETTGRSLLDWQQDVQAPPPLAGVEVERVFMEVPRDELYARAERRFDQMINA
                     GALGEVEKVITYDPALPMMKAIGLPELAAHLRGETTLEEAVALAKTATRQYIKRQLTW
                     WRGQMGHWRDEH"
     gene            2596..3465
                     /gene="serB"
                     /locus_tag="AC16A0_00420"
     CDS             2596..3465
                     /gene="serB"
                     /locus_tag="AC16A0_00420"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012758232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR3103817.1"
                     /translation="MDSVLVLIAAPGSKAIGAEVLGLLRGLGAGEPHWLAAGEAAEFH
                     DFERLSEFMVDIRRFPIDAAVVSIHNRRKRILIADMDSTMIRQECIDELGEAAGVGDR
                     IRDITRRAMRGELDFDAALRERVGLLKGLDAAVIDLIIRERITYMPGGKTLIATMKAH
                     GAHTALVSGGFVQFTARVAAELGFDENQANRLVIENGTLAGTVTDPILGQQAKVEALR
                     RIASARGLEPADAIAVGDGANDLAMLSEAGLGVALHAKPHVQDGSLVRINHGDLTALL
                     YLQGYGRDRFVAA"
     gene            3570..>4864
                     /locus_tag="AC16A0_00425"
     CDS             3570..>4864
                     /locus_tag="AC16A0_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR3103818.1"
                     /translation="MTKKFLLGEERIPKAWYNIAADLPQALPPVLHPGTMKPVGPDDL
                     APLFPMALIMQEVSQEREIEVPEPVRDIYRMWRPTPLYRATALEKALGTTAKIYYKYE
                     GVSPAGSHKPNTAVPQAFYNKQEGVTKLVTETGAGQWGSSLSMAGALFDMQVKVYMVK
                     VSYNQKPYRRALMETYGGVCVASPSTETNAGRQILAADPNSNGSLGIAISEAVEVAAT
                     NPDTKYALGSVLNHVLLHQTVIGIEAIEQMEQAGAAPDIIVGCTGGGSNFAGLTFPFI
                     GQQLRGGKKVRVIAAEPAACPSLTRGKYAYDFGDTSHMTPLVKMHTLGSTFMPPGFHA
                     GGLRYHGMAPLVSHLKELGLIEAEAHTQNSCFEAGILFARTEGIVPAPEATHAVRGAI
                     NAALEAKKNGTSPVILFGLCGHGHFDLSAYDAYLAGTLE"
BASE COUNT          813 a         1635 c         1584 g          832 t
ORIGIN      
        1 gaaatggatt tccacacgcg gatcatgtct tccagtgcat gggccacgtc gccgaccacg
       61 ggaatgtcga cccgcaccgt cttgttgatg gaggacgggt cgatgtcgat gtggatcttc
      121 ttcgagcctg gcgagaaggc attgaggcgg ccggtgatgc ggtcgtcgaa acgcgcgccg
      181 atgttgatca tcacgtcgca gtcgtgcatg gacatgttgg cttcgtacat gccgtgcatg
      241 ccgagcatgc ccagccagtt cttgcccgcc gccggatagg cgccgagccc catcagcgtg
      301 gaggtgatcg ggatattggt gagcgccacg aattcgcgca gcagcttcga cgcggtcggc
      361 cccgaattga tcacgccgcc gccggtgtag agcaccgggc gcttggccgt ggcgatgagc
      421 tccaccgcgg cacggatggc gttctggtcg ggcttcacct tgggccggta ggtcttgtgc
      481 tcgatgttct tggggccgac atacttgccg gtggcgaact gcacgtcctt cggaatgtcg
      541 accaccaccg ggccgggacg gccgtgggtg gcgacataga aagcctcgtg caggatgcgg
      601 gcgaggtcgt tcacgtcctt caccaggtaa ttgtgcttgg tgcagggccg cgtgatgccc
      661 accgtgtcgc attcctggaa ggcgtcgttg ccgatgagat gggtgggcac ctggccggtg
      721 atgcagacca tggggatgga atccatcagc gcgtcggtga ggccggtcac cgcattggtg
      781 gcgccgggtc cggaggtcac caggaccacg ccgcacttgc cggtggagcg ggcatagcct
      841 tccgccatgt gggtggcgcc ctgctcgtgg cggacgagga tgtgcttcac cttctcctgc
      901 tggaagatct cgtcatagat cggcaggacc gcgccgccgg ggtagccgaa gacatgctcg
      961 accccctggt cggccagcgc cttcagcacg atctcggcac ccgtcatctc attcgtggcc
     1021 atcgcaaacc tctttatctc tgtcccgttt cgtcaaacat gcatttcgag ttcaggcaat
     1081 aaaaaaggcc cccaagggag cctgatttcc gcgcagcacc accagatcct ggaggcttaa
     1141 gcctccccgg cgctgcgcgg tcctacgacg agaatgatgt tcatgggcgg gggttgtacg
     1201 tggacggaag aaaaaggtca aggcatctga tagcgggcaa ggaaatcatg gagcggcgac
     1261 aggcttccgg gatcgccggc ccgctccatc tgcgcaaagt ccaccgctgc gacctcatgg
     1321 tcacggagca cggccagacc ggcacggtcc tccgccggcg ccagggcgat gatctcgtcg
     1381 aactccacga cataggccgg gaagtccctg gccatttccg ccaccagcat ggcccagctc
     1441 tggccgcgcc gccgttcggc ctcggcacgc ccggcggccc tcagggtggc ggacggctgg
     1501 ggttcgatgc cgtaatgaac gaccagcggc cggatcgcgg cggccgtcac cgcttcgatg
     1561 cgggcgagca gcatcagcgc ctcatgcggc cggccctcga agagacttgc gagatgggcg
     1621 aaataggccg ctccggagat ctcctcctcg taggacagct ggagggcggc ccggacgtcg
     1681 gtatagcgcc gcggcgccag ggcgtggtcc gttccgcttg tacttgtatc agtgttcatc
     1741 gcgccaatgt cccatctgcc cgcgccacca ggtcaactgg cgcttgatgt actgccgggt
     1801 tgccgtcttg gccagcgcca cggcttcctc cagcgtggtc tcgccccgca gatgggcggc
     1861 gagttcggga aggccgatgg ccttcatcat cggcagcgcc ggatcgtagg tgatgacctt
     1921 ctcgacctcg cccagcgcgc cggcattgat catctggtcg aagcgccgct cggcgcgggc
     1981 atagagctcg tcgcgcggca cctccatgaa gacgcgttcg acctcgacgc ctgccagcgg
     2041 cggcggggcc tgcacgtcct gctgccagtc cagcagcgag cgcccggtgg tctcgaccac
     2101 ctcaagcgcg cggacgatgc gctgccggtc ctgcgggcgc aggcgccgcg ccatttcggg
     2161 atcgcgcgcc gcgagttcgc cgtgaagatc gccatcgtag ctgcgccagt ggtcgcggat
     2221 ctcctgcgag accggcggga tgctcgacag gcccttctcc agcgcgcgga aatagaggcc
     2281 ggtgccgccg gtgatgatcg gcaggcggcc ttcgcgccag ctgtccgcga tggcctgcgc
     2341 gacatcctgc agccagcgcg ccacggtgta accttcgctg ccggggacgt gcccgtagag
     2401 acggtgcggc gcctgcgcca attcctcctc cagcggccgc gcggtgagaa tgcggagctc
     2461 gcggtagacc tgcagcgcat cggcattgat gatgacgcca ttgaggtttc gcgccacgtc
     2521 catcgccagg gcggacttgc cgctggcggt cggtcctgca ataagcagcg cgcgcttgtg
     2581 aggtgtcttg cccatatgga ttccgtcctc gttctcatag cagcccccgg ctcgaaggca
     2641 atcggcgcgg aagtgcttgg cctgttgcgc ggcctcggcg ccggagagcc gcactggctg
     2701 gccgcgggcg aggccgcgga attccacgac ttcgagcgcc tctcagaatt catggtcgac
     2761 atccggcgct tccccatcga tgccgccgtg gtctcgatcc acaaccgccg caagcgcatc
     2821 ctgatcgccg acatggactc gaccatgatc cggcaggaat gcatcgacga acttggtgaa
     2881 gccgcaggcg tcggcgaccg tatccgcgac atcacgcggc gcgccatgcg cggcgaactg
     2941 gatttcgatg cggccctgcg cgaacgcgtc ggcctgctga aaggtctcga cgcggcggtg
     3001 atcgacctca tcatccggga acggatcacc tacatgccgg gcggcaagac cctcatcgcc
     3061 acgatgaagg cgcatggcgc ccataccgcc ctggtctccg gcggcttcgt ccagttcacc
     3121 gcgcgggtgg cggcagagct tggcttcgac gagaaccagg ccaatcggct ggtgatcgag
     3181 aacggcacgc tggcgggcac cgtgaccgac cccatcctcg gccagcaggc caaggtcgag
     3241 gcgctgcgcc ggatcgcttc ggcccgcggc ctcgagcccg ccgatgccat cgcggtgggc
     3301 gacggcgcca atgatctggc catgctgtcg gaagcgggac ttggcgttgc cctgcatgcc
     3361 aagccgcatg tgcaggacgg ctcgctggtc cgcatcaacc atggcgatct cacggccctg
     3421 ctctatcttc agggctatgg ccgcgaccgc ttcgtggcgg cctgacgcgg cgtcgccgca
     3481 gggccacaac cttgccgcgc tgcgcctttc ttctcacttg aaactccccg gccgctgggc
     3541 aaaccttggc gcattgcgga ggatcccaga tgaccaagaa gttcctgctc ggcgaagagc
     3601 gcatccccaa ggcctggtac aacatcgcgg ccgacctgcc ccaggcactg ccgcccgtgc
     3661 tgcatcccgg cacgatgaag cccgtcggcc cggacgatct ggcgcccctt ttcccgatgg
     3721 cgctgatcat gcaggaggtt tcgcaggagc gcgagatcga ggtgcccgag cctgtgcgcg
     3781 acatctaccg catgtggcgt cccacgccgc tgtaccgcgc caccgcgctg gagaaggcgc
     3841 tggggaccac ggccaagatc tactacaagt acgaaggcgt ttcgcccgcc ggttcgcaca
     3901 agccgaacac ggcggtgccg caggccttct acaacaagca ggaaggcgtc accaagctgg
     3961 tcaccgagac gggtgccgga caatggggct cgtcgctctc catggccggc gcgctcttcg
     4021 acatgcaggt caaggtctac atggtgaagg tgtcctacaa ccagaagcct taccgccgcg
     4081 cgctgatgga gacctatggc ggcgtgtgcg tcgccagccc ctcgacggag acaaacgccg
     4141 gccgccagat cctggccgcc gatcccaact ccaacggctc gctgggcatc gcgatttccg
     4201 aagccgtcga ggtggcggcc accaaccccg ataccaaata cgcgctgggc agcgttctga
     4261 accacgtgct gctgcaccag acggtgatcg gcatcgaagc catcgagcaa atggaacagg
     4321 cgggtgctgc acccgacatc atcgtgggct gcaccggcgg cggatcgaac ttcgccggcc
     4381 tcaccttccc cttcatcggc cagcagctgc gcggcggaaa gaaagtgcgc gtgatcgcgg
     4441 ccgaacccgc cgcctgcccc tcgctcaccc gcggcaaata tgcatatgac ttcggcgaca
     4501 cctcgcatat gacgccgctg gtgaagatgc acacgctggg ctccaccttc atgccgcccg
     4561 gcttccatgc cggcggcctg cgctatcacg gcatggcccc gctggtctcg catctgaagg
     4621 aactggggct gatcgaggcc gaggcccata cgcagaattc ctgcttcgag gcgggcatcc
     4681 tcttcgcccg caccgagggc atcgtgccgg cgccggaggc cacccacgcg gtgcgcggcg
     4741 ccatcaatgc ggcgctggaa gccaagaaga acggcacctc gccggtgatc ctcttcggcc
     4801 tctgcggcca cggccacttc gacctctcgg cctacgacgc ctacctggcc ggcacgctcg
     4861 agga
//
LOCUS       DBNGVR010000019         2154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2364456, whole genome shotgun sequence.
ACCESSION   DBNGVR010000019 DBNGVR010000000
VERSION     DBNGVR010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2364456"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="AC16A0_00430"
     CDS             <1..969
                     /locus_tag="AC16A0_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3103819.1"
                     /translation="VIEARDASTSVNLTGRIEAEDEVPLAFRISGRLLENNGKLGARL
                     QTGDLVARLESQNEENQLRAAAAALSAAEAQYNQARGHYERQQTLFRQGWATRANYEL
                     AEKGVRTAASQVEAAQAQMDAAEDLVGFTELRADQDGVYTAVGPGAGEVVQAGQMIVR
                     VARQDGRDAVFDVPAQIIRSASPDPDIMVSLADDPSVKVRGHIREVAPQADPVTRTFE
                     VKVSLENPPESMRLGATVNGVMQTAGVPTIDIPASALTRSNQAPAVWVVDPAALTVSL
                     RTIEISRHGEYVVSVSDGLNPGDVVVTAGVQALHPGQQVRLLGSQQ"
     gene            966..>2154
                     /locus_tag="AC16A0_00435"
     CDS             966..>2154
                     /locus_tag="AC16A0_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3103820.1"
                     /translation="MNGFNLSEWALKHRSITIYLMIVAVLAGLASYYRLGRSEDPSFI
                     IKTMVVQAAWPGATMEETMKQVTERLERTLQETPHLDFLRSFTRAGVSTIFVNLDGKT
                     NAQQVADTWYQVRKSVGDMRHTLPSGVIGPGFNDEFGDTFGIIYGVTADGFSHRELRD
                     HVETLRSKLLGVKDVSKIELLGIQDEQIFIEFSMEDLANLGVDRSALLAALQAQNTVR
                     PAGLIETDEESISLRVSGSFESEQDIANVNFAVGNRMLRLADIATIRRGYADPPQPMF
                     RVNGVPAIGLAVAMRDGGDILQLGENIKQVMADAKAELPLGVEPILVADQAVTVEGAI
                     SEFMSSLWQAVGIILVVSFISLGVRPGLVIALAIPLTLAIVFVVMDLLGIDMQRISLG
                     ALII"
BASE COUNT          389 a          679 c          709 g          377 t
ORIGIN      
        1 gtcatcgagg cgcgagacgc ctcgaccagc gtgaatctga cgggccggat cgaggccgaa
       61 gacgaagtgc cgctcgcctt ccgcatttcc ggacggctgc tcgagaacaa cggcaagctg
      121 ggggcccggc tccagaccgg cgatctggtg gcacggctgg aatcgcagaa cgaggaaaat
      181 caactccggg ccgccgccgc ggcgctgtcc gcggccgagg cccagtacaa ccaggcgcgc
      241 ggtcactacg agcgccagca gactctgttc cggcagggct gggccacgcg agccaactat
      301 gaacttgcgg aaaagggcgt gcggacggcg gcatcgcagg tggaggccgc ccaggcgcag
      361 atggatgccg cggaggatct cgtcggattt acagaactgc gcgcggatca ggacggcgtc
      421 tacaccgcag tcggcccggg agcgggcgaa gtggtgcagg ccggccagat gatcgtcagg
      481 gtcgcgcgcc aggacggccg cgacgccgtc ttcgacgtcc cggcccagat catccggtcg
      541 gcatcccccg atcctgacat catggtttcg cttgccgatg atccatcggt caaggtgcgc
      601 ggccacattc gcgaagtggc gccccaggcc gatcccgtga cccgcacatt cgaagtgaag
      661 gtcagcctgg agaatccgcc ggagtcgatg cgcctcggcg cgacggtcaa tggcgtgatg
      721 cagaccgcgg gtgttccgac gatcgatatc ccggccagcg cgctcacgcg cagcaaccag
      781 gctccggccg tctgggtcgt ggatcctgcc gcgctgacgg tctcgctgcg caccatcgag
      841 atcagccggc acggcgaata cgtcgtgtcc gtctcggacg gtttgaatcc gggtgacgtg
      901 gttgtgaccg ccggcgtgca ggcgctgcat cccggtcagc aggtccgctt gctcgggtcg
      961 cagcaatgaa cggcttcaat ctctccgagt gggcgctgaa gcaccggtcg atcacgatct
     1021 acctgatgat cgtcgccgtg ctggcggggc ttgcgtccta ctaccgcctg ggccgcagcg
     1081 aggatccttc cttcatcatc aagaccatgg tcgtccaggc cgcctggccc ggcgcgacga
     1141 tggaagagac gatgaagcag gtgaccgagc ggcttgagcg cacgctgcag gaaacgccgc
     1201 atctcgattt cctccgaagc ttcacgcgtg caggcgtgtc cacgatcttc gtcaatctcg
     1261 acggcaagac caatgcgcag caggtcgcgg atacctggta ccaggtgcgc aagagcgtgg
     1321 gcgacatgcg gcataccctg ccctcaggtg tcatcggccc cggcttcaac gacgagttcg
     1381 gcgacacctt tggcatcatc tatggcgtga cggccgacgg cttcagccac cgcgaattgc
     1441 gcgatcacgt cgaaacgctc cggtcgaagc tgctgggcgt gaaggacgtc tcgaagatcg
     1501 agctgcttgg cattcaggac gaacagatct tcatcgagtt ctcgatggaa gatctggcga
     1561 acctgggtgt ggaccgctct gcgctgctgg cagccctcca ggcccagaac acggtgcgcc
     1621 ccgcgggtct catcgagacc gacgaggaat cgatctcgct cagggtctcg ggatccttcg
     1681 aatccgaaca ggatatcgcc aacgtgaact tcgccgtcgg caatcgcatg cttcgccttg
     1741 ccgatatcgc aacgatccgg agaggctatg ccgatccgcc gcaacccatg ttccgggtga
     1801 acggggtgcc ggcgatcggg ctggctgtcg ccatgcgcga cggcggcgac atcctgcaac
     1861 tgggcgaaaa cataaagcag gtgatggccg atgcaaaggc cgaattgccg ctgggcgtcg
     1921 agccgatcct ggtcgcggac caggcggtga ccgtcgaagg cgcgatctcg gaattcatgt
     1981 cgtcgctctg gcaggcggtg ggcatcatcc tggtcgtgag cttcatcagc cttggcgtca
     2041 ggccgggact tgtcatcgcg ctcgcaattc cgttgacgct tgccattgtc ttcgtcgtca
     2101 tggatctgct gggcatcgac atgcagcgca tttcgctcgg tgcgctgatc atcg
//
LOCUS       DBNGVR010000020         3306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1972463, whole genome shotgun sequence.
ACCESSION   DBNGVR010000020 DBNGVR010000000
VERSION     DBNGVR010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1972463"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="AC16A0_00440"
     CDS             complement(<1..198)
                     /locus_tag="AC16A0_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3103821.1"
                     /translation="MHLEDLLLGAFVYLAAAVVAAPIATRLGLGSVLGYLVAGMVIGP
                     SVLGLVGTEGADVMHFAEFGVI"
     gene            complement(198..797)
                     /locus_tag="AC16A0_00445"
     CDS             complement(198..797)
                     /locus_tag="AC16A0_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR3103822.1"
                     /translation="MRILVLFAHPKRQASVVQRAMLRSIDGLDGVTIADLYAEYPDFD
                     IDVAREQQRLLDHDLIVLQHPFYWYSTPAILKEWQDLVLENGWAYGPGGTRLAGRFLM
                     SAISAGGSEEAYRNEGRNRFSIMELLAPFNQTAYLCSMAYLTPFVIYAGRRMTTESLS
                     TSAETYRDLIVGLRDGLINPRGRLAPGFVLPPAFTTGGR"
     gene            complement(905..1183)
                     /gene="sciP"
                     /locus_tag="AC16A0_00450"
     CDS             complement(905..1183)
                     /gene="sciP"
                     /locus_tag="AC16A0_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CtrA inhibitor SciP"
                     /protein_id="HIR3103823.1"
                     /translation="MNGQLRPRVNYVIGPDGSPLTVADLPPPGNQRWVIRRKAEVVAA
                     VRGGLLSLEEACERYVLTVEEYLSWQRSIDRHGLAGLRTTRIQDYRSN"
     gene            1364..2518
                     /gene="mnmA"
                     /locus_tag="AC16A0_00455"
     CDS             1364..2518
                     /gene="mnmA"
                     /locus_tag="AC16A0_00455"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3103824.1"
                     /translation="MSISEGILDRVRDSVGIAGDPAAIRIVCAMSGGVDSSVTAALLK
                     HAGYDVVGITLQLYDHGEAVRRKGACCAGQDIHDARRVAGELGIPHYVLDFESRFREA
                     VIDSFVDSYLAGETPVPCVTCNRTVKFADLLSRARDLDAAAMATGHYIESRARTEGSV
                     RRDLFTPADMDRDQSYFLFATTQDQIDFLRFPLGRLSKPETRAIASGLGLSVAAKPDS
                     QDICFVPEGRYADVILKLRPDGAEPGDIVHLDGRVLGRHEGIIHYTVGQRRGLGIAEG
                     EPLYVVRIDAVARRVVVGPRSALLQREIKVSRLNWLGHDALTEAGQPVHARIRSTRAP
                     MPAEIRRGEPGAVVTISDGEYGVSPGQACVLYDGQGAGARVLGGGYIARA"
     gene            2595..>3306
                     /locus_tag="AC16A0_00460"
     CDS             2595..>3306
                     /locus_tag="AC16A0_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3103825.1"
                     /translation="MAKINLHPNLDRDVKKGVAWAVHAFTTCGIVLGFLALVAVLKND
                     PVAAFMWLGLALFVDGIDGTLARKARVLEYTPNVDGRTLDNVIDFFTYVAVPALMVYW
                     FNMTPVDFIFSGQTWSLISAAAIMAVSCYTFANVGMKSDDYYFVGFPAVWNVVVLYFF
                     VLQSGPIANLVTIVLLCILTFIPIKFVHPLRVTDWREITIPMTVLWAAMSLSLIIQAK
                     DRSGWGLVEDVQLWVWVGA"
BASE COUNT          567 a         1053 c         1063 g          623 t
ORIGIN      
        1 gatcacgccg aactcggcga agtgcatcac gtccgcacct tcggtgccca cgaggcccag
       61 caccgaggga ccgatcacca tgccggccac caggtagcca aggaccgatc ccagccccag
      121 ccgcgtcgcg atgggcgcgg cgacaacggc cgccgcaaga taaacgaaag caccgagcag
      181 gaggtcttcg agatgcatca ccggcctcct gtcgtgaagg ccggtggcag cacaaagccc
      241 ggtgcaagcc ggccacgcgg attgatgagg ccatcgcgca ggcccacgat caggtcgcga
      301 taggtttccg cagacgtgct cagactttcg gtcgtcatgc ggcggcctgc gtagatgacg
      361 aatggcgtca ggtaagccat cgaacagaga taggcagtct ggttgaaggg cgcgagcaac
      421 tccatgatgc tgaacctgtt gcggccttcg ttgcgatagg cttcctcgga accaccggcc
      481 gagattgccg acatcaggaa gcggcccgcc agcctggttc cgccggggcc gtaggcccag
      541 ccgttctcca gcaccagatc ctgccattcc ttgaggatgg cgggcgtcga gtaccagtag
      601 aaagggtgct gcagcacgat gagatcatga tcgagcagcc gctgctgctc gcgcgcgacg
      661 tcgatgtcga agtcgggata ctcggcgtag agatccgcga tggtgacgcc gtccagcccg
      721 tcgatggacc tgagcatggc gcgctggacc acggacgcct ggcgctttgg gtgggcaaac
      781 agcacgagga tcctcatccg tcgtgttttg ccacattagt gggtcctgta aagtgtgggg
      841 cgcgcgcgaa agcaaagggg ccggacgagt gtccggcccc ttttgcgaaa ctctcgtgtg
      901 gcgctcagtt cgagcgatag tcctggatgc gcgtcgtccg caggcctgcg aggccatggc
      961 ggtcgatcga gcgctgccag ctcagatatt cctcgacggt gagcacatag cgctcgcacg
     1021 cctcttccag actcagcaaa ccgccacgga ctgccgcgac cacctccgcc ttgcggcgaa
     1081 tgacccagcg ctggtttcca ggcgggggga ggtcggcgac agtcagtggg ctgccatcgg
     1141 gcccgatgac gtagtttacg cgtggacgca actgaccgtt cattgacaaa ctcaacctaa
     1201 tacgcaggta ctcactgaca gccagactag gcctcgcgcc ttaaaattgg gctaagtaca
     1261 gatgaagaat tggcaaaccg gaccctctcc ggtgctccat tccggaacag gcggggcgaa
     1321 acggctgttt actttcgcgt tgcgccgccc catctagcca tccatgagca tttccgaggg
     1381 cattctggac cgggtgcgtg acagcgtcgg gatcgcgggc gatcccgcgg cgatccgcat
     1441 tgtctgcgcc atgtcgggcg gggtcgattc ttcggtcacc gcggcactgc tgaagcatgc
     1501 gggctacgat gtcgtcggca ttaccctgca actctacgat cacggcgagg cggtccggcg
     1561 caagggcgcc tgctgcgcag gccaggacat ccacgatgcc cgccgcgtgg ccggggagct
     1621 tggcattccg cattacgtcc tcgacttcga gtcccgcttc cgggaagcgg tgatcgacag
     1681 tttcgtggac tcatacctcg ccggcgagac gccggttccc tgcgtcacct gcaaccggac
     1741 ggtcaagttt gcagacctgc tgtcccgtgc ccgcgatctc gatgccgccg ccatggcgac
     1801 cggccattac atcgaaagcc gtgcccgcac ggagggatcg gtccgccgcg atctcttcac
     1861 gcccgccgac atggaccgcg accagagcta tttcctcttc gctaccaccc aggaccagat
     1921 cgacttcctg cgctttccgc tcggccggct gagcaagcct gagacgcgcg ccattgcctc
     1981 cgggctgggg ctttcggtgg cggcgaagcc cgacagccag gacatctgct tcgtgccgga
     2041 gggccgctat gcggatgtca ttctcaaact ccggccggat ggcgctgagc ccggcgacat
     2101 cgtccatctc gacgggcgcg tgctgggacg ccacgagggc atcattcact acactgtagg
     2161 gcagcgccgc ggccttggca ttgccgaagg cgagccgctt tacgtggtgc ggatcgacgc
     2221 cgtggcccgc cgcgtggtgg tcggcccgcg atcggccctg ctgcagcggg agatcaaggt
     2281 gagccggctc aattggctgg gccatgacgc cctgaccgag gccggccagc cggtacatgc
     2341 ccgcatccgt tcgacccgag cgcccatgcc ggccgagatc aggcggggag agccgggtgc
     2401 cgtcgtcacc atttcggatg gcgagtatgg tgtctcgccc gggcaggcct gcgtgctcta
     2461 tgacgggcag ggggccggcg ccagggtgct tggcggcggc tacatcgcgc gcgcctgacg
     2521 catggtgaag cggcgtttgc tttgcccatc gggcctacct ataatgactt ttctgagtgg
     2581 gagttgccga ggccgtggcc aagatcaatc tgcatccgaa cctcgaccgg gatgtgaaga
     2641 agggggtcgc ctgggccgtc cacgctttca ccacctgcgg catcgtgctg ggtttcctgg
     2701 cgctggtggc ggtgctgaag aacgatccgg tcgccgcctt catgtggctg ggcctcgcgc
     2761 tctttgttga cggcatcgat ggaaccctgg cgcgcaaggc cagggtgctt gaatacacgc
     2821 ccaacgtcga cggccgtacc ctcgacaatg tcatcgactt cttcacctat gtcgccgtgc
     2881 cggcgctgat ggtttactgg ttcaacatga cgccagtgga cttcattttt tccggccaga
     2941 cctggagcct catctcggcg gccgcgatca tggcggtctc gtgctacacc ttcgccaatg
     3001 tcggcatgaa gtccgacgac tactacttcg tcggctttcc ggccgtctgg aacgtggtgg
     3061 tgctctattt cttcgttctg cagtctggcc ccatcgccaa tctggtcacc atcgtgctgc
     3121 tctgcatcct gaccttcatt ccgatcaagt tcgtgcatcc gctgcgtgtc accgactggc
     3181 gggagatcac cattccgatg acggtgctgt gggcggccat gtcgctgagc ctcatcatcc
     3241 aggccaagga ccggagcggc tggggcctcg tcgaggacgt gcagctctgg gtgtgggtgg
     3301 gggcca
//
LOCUS       DBNGVR010000021         4355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3543443, whole genome shotgun sequence.
ACCESSION   DBNGVR010000021 DBNGVR010000000
VERSION     DBNGVR010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3543443"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..707)
                     /locus_tag="AC16A0_00465"
     CDS             complement(150..707)
                     /locus_tag="AC16A0_00465"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3103826.1"
                     /translation="MAAIPPVCDFGWKAIDFTLPGVDGKSYRLADSAGPNGTLIAFIC
                     NHCPYVKAVIGRLVRDAAELRAHGIETLAISSNDATAYPEDSFPEMKVFAERHGFGFP
                     YLHDDAQDVARAYGAACTPDFFGFNSKLELQYRGRLDASRKDTLPDAKRELYEAMRRI
                     AETGRGPAEQVVSIGCSIKWKSFSH"
     gene            complement(722..1282)
                     /locus_tag="AC16A0_00470"
     CDS             complement(722..1282)
                     /locus_tag="AC16A0_00470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3103827.1"
                     /translation="MNTLASLPAVETRQLVLREIEARDAEAFAGFMMQEPYQRYIAMR
                     LASEAEVRAFVTRSVARQGDERRNVFHLAAEEKYSGEAVGDGFLILQRPKSLEIGWGV
                     HPAMWRMGFGTEIGRALLGLAFERLRADRVWCKIMSGNKASMMLAKRIGLRHMQSHAD
                     FPAGQGRFGAVEFYGLSAEQYFDMAY"
     gene            1409..1714
                     /locus_tag="AC16A0_00475"
     CDS             1409..1714
                     /locus_tag="AC16A0_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103828.1"
                     /translation="MTAMTRLALTCLLVAATGLEAGVALAKGKAFCRGYAEEIANALA
                     GPRDGGSGAEADGGMGLGFTSDAGAASDAVSPVTRGDAGSDAWRRTYRAAYSECRAS"
     gene            1765..3396
                     /locus_tag="AC16A0_00480"
     CDS             1765..3396
                     /locus_tag="AC16A0_00480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3103829.1"
                     /translation="MTDLIIVNGDIRTMDPLQPRVTALAARGRRVMALGDDAEIRALA
                     TPSTRVIDAGGRLVLPGFQDTHIHLQESGTRHAFDLDLQGVRNLDELKGKLATFAAKH
                     PDRAWLKGHGWYTGIFNDRNLTRQVLDEAVPDRPLFLSSSDYHSAVINTRACEAIGLD
                     AGVQDPENGYFSRDALGIPTGMLNEAAMEFARERMPKTADAEWEEGVRWGQHHANRHG
                     FTGVLDALVTERHLRVYNALDRRGELTLRVASTALVEPSDSVKGALDRLEDFRRSYKS
                     EMLKVHSAKFFLDGVFENRTAAMIDGYKDEIGGNAPVMFGENHLRELFIAMDAARFQI
                     HVHVIGDNAARAALDGLEAAREVNGEWPSLHQLAHVQLVRPEDIPRFGKLGVMANIQP
                     LWARNEPSVTDVAVPMAGKDLARWMYPFRSIIDAGGSFAMSSDWGVSTLDPFQIMQTA
                     ITRQPPEKGRNHPVFLPEERMTLDECIKGYTVNAAAAAWRSPDTGSLERGKFADVIIL
                     DQNLFEIDVYDIHKTKVMLTLLGGKEVYRHDGFAG"
     gene            complement(3519..4304)
                     /locus_tag="AC16A0_00485"
     CDS             complement(3519..4304)
                     /locus_tag="AC16A0_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3103830.1"
                     /translation="MATPIRSAFSVEGEGEPLFLIHGIGASRASFAGLVPHLKRDFRV
                     ISYDLRGHGASPVPKPPYTLDDLVEDLEALRRELHIERAHFAGHSLGGMIGPAYARKY
                     PQHVKTLGLWSTAAFRTEDDSAKVKGVVAAMRAKGIPQVLEALKDRWFTPEFAARRPD
                     VIERRMQQVIDTDPDVFLSVFDIYAETEMAPWLHEIAQPCQVLTGENDGGCNPRLNKL
                     IAEALPDSELVILPVLRHAILLEAADQVAPPVLDFLRRHKGRG"
BASE COUNT          794 a         1447 c         1358 g          756 t
ORIGIN      
        1 ggaacgctcg gcgagttctt tctggccaag acctagcagg gcgcgcgcgg cgcgcatctg
       61 gccggaagtg atcatgatgc ggtcaaccca tgcattatga tgtaccaagc atcatattga
      121 ccgcagatta cctcagtaat gagggaaaat caatgcgaga acgacttcca cttgatcgag
      181 cagccgatcg agaccacctg ctcggccggt ccacggcccg tctcggcgat ccggcgcatg
      241 gcctcgtaca actcgcgctt cgcatccggt aacgtatcct tgcgcgaggc atcgagccgg
      301 ccccggtact gcagctccag cttgctgttg aagccgaaga aatccggcgt gcaggccgcg
      361 ccataggcgc gggccacgtc ctgggcatcg tcatgcaggt agggaaatcc gaaaccatgc
      421 cgctcggcaa agaccttcat ctcggggaaa ctgtcttccg gataggccgt ggcgtcgttg
      481 gaggagattg ccagcgtttc gatgccatgc gcgcgcagct ccgccgcatc gcgcaccagc
      541 cgcccgatca cggccttcac gtagggacag tgattgcaga tgaaggcgat caacgtgccg
      601 ttcggaccgg cgctgtcagc cagccgatag cttttgccat cgacgcccgg gagcgtgaag
      661 tcgattgcct tccatccgaa atcgcagaca ggtgggatcg ccgccatggg atcgattcaa
      721 ttcaataagc catgtcgaag tactgctcgg cactcaaccc gtagaactcg accgcaccaa
      781 accgaccctg ccccgccgga aaatcggcat gcgactgcat gtgccgcagg ccgatacgct
      841 tggccagcat catcgaggcc ttgttgccgc tcatgatctt gcaccacacg cggtcggccc
      901 gcaggcgttc gaaggcgagg cccagaagcg cccggccgat ctcggtcccg aaacccatgc
      961 gccacatggc aggatgtacg ccccagccga tctccagaga cttcggccgc tgcaggatga
     1021 ggaagccgtc gcccactgct tcgccgctgt atttctcttc ggcggccagg tgaaacacgt
     1081 tgcgccgctc gtcgccctgg cgcgccaccg accgggtgac gaaagcgcgc acctcggcct
     1141 cgctcgccag ccgcatggcg atgtagcgct gatagggctc ctgcatcatg aagccggcaa
     1201 aggcctcggc atcgcgcgct tcgatttccc gaagcaccag ttgccgggtt tccacggcgg
     1261 gcagactcgc aagcgtgttc ataaaggaat tgttaacgca atcctgcgtc atctgaaaga
     1321 gcttgctgca tcttgcctga atcgtgccgc ctattggcgc cacattctgc ggttaaccat
     1381 cggggcagga cggccggagg acgacagaat gaccgcgatg actcgcctgg ccttgacttg
     1441 ccttctggtc gccgcaaccg gccttgaggc cggcgtcgcc ctcgccaagg gcaaggcctt
     1501 ctgccgcgga tacgccgaag aaatcgccaa tgcccttgcc ggcccgcgtg atggcgggtc
     1561 cggtgcggaa gctgatggcg gaatgggcct cggcttcacc tcggatgccg gtgccgcatc
     1621 ggacgcggtc tctccggtca cgcgtggcga tgccggcagc gacgcatggc gccgcaccta
     1681 tcgcgcggcc tattcggaat gccgcgcctc ttgagccatc cgtcctgact gggcatagtt
     1741 tcccggaact gaccgggaga catcatgaca gacctcatta tcgtcaacgg cgacatccgc
     1801 accatggatc cgctgcaacc gcgcgtgacg gcgctcgcag cacgcggaag acgcgtcatg
     1861 gcgctcggcg acgacgcgga gatccgcgcg ctggcgacgc cgtcgacccg cgtgatcgat
     1921 gcgggtgggc ggttggtgct gccgggcttt caggacacgc acatccatct gcaggaaagc
     1981 ggcacccgcc atgccttcga tctcgacctg cagggcgtgc gcaatcttga cgaactgaag
     2041 ggcaagctcg caaccttcgc ggcgaagcac cctgaccgcg cctggctcaa ggggcacggc
     2101 tggtacacgg gcatcttcaa cgaccgtaac ctgacgcggc aggtgctcga cgaggctgtt
     2161 ccggaccgcc cgctgtttct atcctcgtcc gactatcact ccgccgtcat caacacgaga
     2221 gcctgcgaag cgatcgggct cgacgctggg gtccaggatc ccgagaacgg ttatttctcg
     2281 cgtgatgcgc tgggcatacc caccggcatg ctcaacgaag cggctatgga gttcgcacgc
     2341 gagcgcatgc cgaagaccgc tgatgccgag tgggaagagg gcgtgcgctg gggccagcat
     2401 cacgccaacc ggcacggctt caccggcgtg ctcgatgccc tggtcaccga gcgccatctg
     2461 cgcgtctaca acgcgctcga ccggcgcggt gagctgacgc tccgcgtggc ctcgaccgcc
     2521 ctggtcgagc cgtccgacag cgtcaagggc gcgctcgacc gcctggagga cttccgccgc
     2581 agctacaagt cagagatgct caaggtgcac tcggccaagt tctttctcga tggagtcttc
     2641 gagaaccgca cggccgcgat gatcgacggc tacaaggacg agatcggcgg caatgcgccc
     2701 gtcatgttcg gcgagaacca tctgcgcgaa ctcttcatcg ccatggatgc ggcgcgtttc
     2761 cagattcacg tccatgtcat cggcgacaat gcggcgcgcg cggcgctcga cggcctcgaa
     2821 gctgcccgcg aggtcaacgg cgagtggccc agcctgcatc agctggcgca tgtgcagctg
     2881 gtcaggcccg aggacattcc gcgcttcggc aagctcggcg tcatggccaa catccagccg
     2941 ctctgggccc gcaatgaacc gtcggtcacc gacgtcgccg tgccgatggc aggcaaggat
     3001 ctcgcgcgct ggatgtatcc cttccgctcc atcatcgatg ccggcggcag tttcgccatg
     3061 tccagcgact ggggcgtttc caccctcgac ccgttccaga tcatgcagac cgccatcacc
     3121 cgccagccgc cggagaaggg ccgcaaccat cctgtcttcc tgcccgagga acgcatgacg
     3181 ctggacgagt gcatcaaggg ctacaccgtc aacgcagccg ccgccgcgtg gcgctcgccc
     3241 gacaccggct cgctggaacg cggcaagttc gcggacgtga tcatcctcga ccagaatctc
     3301 ttcgagatcg acgtctacga catccacaag accaaggtca tgctcacgct gctcggcggc
     3361 aaggaagtct accgccacga cggtttcgcg ggttaggacg ccagcctatt gccgcttcgc
     3421 cggggtgacg gcttgaatgg gacggcttca gaaggaacct ctcgtcttca ctcgcggcat
     3481 gaagatccct ccgtctacgg ataaagggga tacctgtgct atcccctgcc cttgtgtcgg
     3541 cgcaggaagt cgaggaccgg cggggccacc tggtcggcgg cttccagcag gatggcgtgg
     3601 cgcagcaccg gcaggatcac cagctcggag tccggcaagg cctcggcgat caacttgttc
     3661 aggcgcggat tgcagccgcc gtcgttctcg ccggtcagca cctggcaggg ttgcgcgatc
     3721 tcgtgcagcc agggcgccat ctcggtctcg gcatagatgt cgaagacgga gagaaaaaca
     3781 tccggatcgg tgtcgatcac ctgctgcatg cggcgctcga tcacgtccgg ccgccgcgcc
     3841 gcgaactcag gcgtgaacca gcggtccttg agcgcctcca gcacctgcgg gatgcccttg
     3901 gcgcgcattg ccgccaccac gcccttcacc ttggcgctgt cgtcttcggt gcggaaggcg
     3961 gcggtggacc acaggcccag cgtcttcacg tgctgcggat acttgcgcgc ataggccggg
     4021 ccgatcatgc cgcccagcga gtgcccggcg aaatgcgcgc gctcgatgtg cagctcgcgg
     4081 cgcagcgctt ccagatcctc gacgaggtcg tccagcgtat agggcggctt cggcaccggc
     4141 gaagcgccat ggccacgcag gtcgtagctg atcacgcgga agtcgcgctt cagatgcggc
     4201 acgagtccgg cgaaggaggc acgcgaggcg ccgatgccgt ggatcaggaa aagcggctcg
     4261 ccctcccctt cgaccgaaaa tgcggaacgg atcggcgtgg ccattgcgct tacttgtccg
     4321 ggtatttctc gttcatcatg tcgtagccgg tgccc
//
LOCUS       DBNGVR010000022         2574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2758473, whole genome shotgun sequence.
ACCESSION   DBNGVR010000022 DBNGVR010000000
VERSION     DBNGVR010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2758473"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1512
                     /locus_tag="AC16A0_00490"
     CDS             52..1512
                     /locus_tag="AC16A0_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HIR3103831.1"
                     /translation="MARGKTAKKTLTSLTDVYAFFRQNQTPITFLSPTPYNILGLGRW
                     INKFEYINFFDSFDGSHPKITVPREHGPHEFRSIEDVNNYLLRHKEVRAKLKERGPGY
                     LLLVMFDEETEALARELGQKIALPPAKLRKHIDSKITTTRLGNEAGIASAPNTMGRAR
                     SFRELMKLAAAARLGSDLVVQTPYGDSGRTTFFIKSEEDWKKNQDVLAKEQLKVMRRI
                     NHLPGTVEAVATRHGTLVGPMQTDITGFGEVTPYKGGWCGNDVFTGGFETERKAVQRM
                     AQRLGDRLYKAGYKGAFCMDFLIDTDTRKVYLGEINPRVSGASPMTNLITSTYGGCPV
                     YMFHLLEFMGVDWELDLSSVQQRWAEFDNWSQLILKYPVDRVEMITKAPASGIWRMND
                     KGDISLVRKSIDWFLVSGEDEAFYLRVYTAGDYRYHGADMGILVSRGRFQTDDRKLTD
                     RAKAWVSAIHRQFEAIPVSGSSAPVPPPMNDSNKMF"
     gene            1515..2528
                     /locus_tag="AC16A0_00495"
     CDS             1515..2528
                     /locus_tag="AC16A0_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3103832.1"
                     /translation="MPKLADHLTLETCLPQSGLLVGRVWNPGEDGPSVVRVDGDRVLD
                     ITASFPTMRDLCETDDPAGSARAAEGEIMGGLAAILANTAPETRNAAHPWLLPPIDLQ
                     CVKAAGVTFATSMLERVIEERARGNPEAAADIRKSIGELIGDDLSKLKPGSPQAMKLK
                     QVLIEKGVWSQYLEVGIGPDAEIFTKSQVLSSVGHGMQAGLNAISTWNNPEPEVVVVV
                     ASSGRIVGATLGNDVNLRDVEGRSGLLLGKAKDNNASCAIGPFIRLFDGDFTIDSVRH
                     MQLTLTVEGEDGYVLKGGSNMSRISRDPEDLVGQMLNASHQYPDGAVLFLGTLFAPVE
                     DRD"
BASE COUNT          521 a          845 c          767 g          441 t
ORIGIN      
        1 tagattaagc cccttgagag cgcgccaagc gcctgagggg agttgccaag aatggcccgg
       61 ggaaagaccg ccaagaagac actcacgagc ctgaccgacg tctatgcctt cttccggcag
      121 aaccagacgc ccatcacctt cctgtccccc acgccctaca acatcctggg tctggggcga
      181 tggatcaaca agttcgaata catcaatttc ttcgacagct tcgacggcag ccatcccaag
      241 atcacggtgc cgcgcgaaca cggtccgcat gagttccggt ccatcgagga cgtaaacaac
      301 tatcttctgc gccacaagga agtccgcgcg aaactcaagg agcgcgggcc tggctacctg
      361 cttcttgtca tgttcgacga agagaccgag gcgctggcgc gcgaactggg ccagaagatc
      421 gcactcccgc cggcaaagct gcgcaagcac atcgactcca agatcaccac caccaggctc
      481 ggcaacgaag ccggcatcgc cagcgcgccc aacaccatgg gccgcgccag atcattccgt
      541 gagctgatga agctggcggc ggctgcgcgc ctcggcagtg accttgtggt tcagacgccc
      601 tatggcgatt ccggccgtac caccttcttc atcaagtcgg aggaggactg gaagaagaac
      661 caggacgttc tggccaagga acagctcaag gtgatgcgcc gcatcaacca cctgccaggt
      721 acggtcgagg ccgtggccac acgccacggc acgctggttg gccccatgca gacagatatc
      781 accggcttcg gcgaagtgac cccctacaag ggcggatggt gcggcaacga cgtcttcacc
      841 ggcggcttcg aaaccgagcg caaggcggtg cagcgcatgg cgcaaaggct cggcgaccgg
      901 ctctacaagg ccggatacaa gggagccttc tgcatggact tcctcatcga tacggacacc
      961 cgcaaggtct atctcggcga gatcaacccg cgcgtgtcgg gcgcatctcc aatgaccaat
     1021 ctgatcacgt cgacctatgg tggctgccct gtctacatgt tccatctcct tgagttcatg
     1081 ggcgtggact gggaactcga tctgtcgagc gtccagcagc gctgggccga gttcgacaac
     1141 tggagccagc tgatcctcaa gtatccggtc gatcgcgtcg agatgatcac gaaggcaccc
     1201 gcctccggaa tctggcggat gaacgacaag ggcgacatct cgctggtgcg caagtcgatc
     1261 gactggttcc tcgtctcggg cgaggatgag gctttctacc ttcgcgtcta cacggcaggc
     1321 gattaccgct accacggcgc cgacatgggc atcctcgtgt cccgcggccg ctttcagacg
     1381 gatgaccgca agctcaccga tcgcgccaag gcctgggtct cggccattca ccggcagttc
     1441 gaggcgatcc ccgtgtcggg atcttctgcg ccggtgcctc cgcccatgaa cgacagcaac
     1501 aagatgttct gatcatgccc aagcttgccg accatctcac cctagagacc tgcctgccgc
     1561 aatctggcct gctcgtcggg cgcgtctgga accctggcga agacggaccc agcgtggtgc
     1621 gcgtcgacgg cgaccgggtc ctcgacatca cggcgagctt tcccaccatg cgcgaccttt
     1681 gcgagacgga tgatccggcc gggtcggccc gcgccgccga gggcgaaatc atgggcggtc
     1741 tcgccgccat actcgccaac acggctcccg aaacgcgaaa cgcggcgcat ccctggctgc
     1801 tgccgcccat tgacctgcaa tgcgtgaagg ccgcgggcgt gacctttgcc acttccatgc
     1861 tcgagcgcgt gatcgaggaa cgcgcccgcg gcaatccgga ggccgccgcc gatatccgca
     1921 agagcatcgg tgaactcatc ggcgacgacc tgtcgaagct caagcccggc tcgccgcagg
     1981 ccatgaagct gaagcaggtt ctgatcgaaa agggtgtctg gtcgcagtat ctcgaagtcg
     2041 gcatcggccc tgacgcggag atcttcacga aatcgcaggt cctgtcatcg gtcgggcatg
     2101 gcatgcaagc cggcctgaac gcgatctcga cctggaacaa tcccgagccc gaagtggtgg
     2161 tggtggttgc gtccagcggc cgcatcgtcg gcgccacgct tggcaacgac gtgaacctgc
     2221 gcgatgtcga aggccgttcc ggcctcttgc tcggcaaggc caaggacaac aacgccagtt
     2281 gcgccatcgg acccttcatc cgacttttcg atggcgactt cacgatcgac agcgtgcggc
     2341 acatgcagct gacacttacc gtcgagggcg aggatggcta tgtgctcaag ggcggctcga
     2401 acatgtcccg catcagccgg gatccggaag atctcgtcgg ccagatgctg aatgccagcc
     2461 accagtatcc cgacggcgcg gtcctctttc tcggcacgct cttcgcgcca gtcgaagacc
     2521 gcgactaagc ggacaagggc ttcacccaca agctgggcga catcgtctcc gtgg
//
LOCUS       DBNGVR010000023         4269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3937154, whole genome shotgun sequence.
ACCESSION   DBNGVR010000023 DBNGVR010000000
VERSION     DBNGVR010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3937154"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="AC16A0_00500"
     CDS             complement(<1..247)
                     /locus_tag="AC16A0_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3103833.1"
                     /translation="MVHFSASTAKSARARLKRWLPVMILGGALLAFYLAGLQRYLSFE
                     SFAAHELQIQDFVARHLILSVLIYMAVYVAVVALSLPG"
     gene            500..634
                     /gene="rpmH"
                     /locus_tag="AC16A0_00505"
     CDS             500..634
                     /gene="rpmH"
                     /locus_tag="AC16A0_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3103834.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARMATVGGRNVLARRRAKGRKRLSA
                     "
     gene            654..1007
                     /gene="rnpA"
                     /locus_tag="AC16A0_00510"
     CDS             654..1007
                     /gene="rnpA"
                     /locus_tag="AC16A0_00510"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3103835.1"
                     /translation="MERLKRRQDFVAAARASYAAMPGLTVQARDRGDDGSPRVGFTAT
                     KKLGNAVVRNRVKRRLREIARQHLAAAALPGHDYVLIGRQPAFARAYADLEKDFTSAL
                     KRLHHPRRPVEKDPS"
     gene            1004..2803
                     /gene="yidC"
                     /locus_tag="AC16A0_00515"
     CDS             1004..2803
                     /gene="yidC"
                     /locus_tag="AC16A0_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR3103836.1"
                     /translation="MKHDNKNFILAIVLSMAIIFGWQYFYAMPQADRLAEQARQAEQA
                     TGQQPATPTAPSSGAPAGQVPGTAGAGVVPRDEALASSPRVKIDTPFVSGSINLKGAQ
                     VDDLHLLRYRETIDPKSPTITLLSPAGTVGATFAEQGFIPASGVSAKLPTPETVWTAP
                     PGATLTESTPLVLTWDNGEGLVFTRTISIGDDYLFSVTQSVENRGAQPVSLFPFARVI
                     RQDTPVVAGYWVFFEGMLGVADGALQEVHYSDASETTEPVRVDSTGGWLGFTDKYWAA
                     VAIPDQTRAVTTTYAHEKLGGRDVYQTNYVARDAMTVAPGASASYQDQLYAGAKVVSI
                     INTIGERYKIDRFDLMIDWGWFRFLTKPMFYLIDYLQKLVGNFGVAILLATVLVKLLV
                     FPLANKSYASMSKMKKLQPEMLKLKELYPDDKMKQQQEMMALYKREKVNPMSGCLPVL
                     VQIPIFFALYKVILTSIELRHAPFFGWIHDLSAPDPTSLFNLFGLIPWTPPAMLMIGI
                     WPIIMGITMWIQMRLNPAPPDPVQASLFNWMPVLFTFMLGTFPAGLVIYWAWSNTLSI
                     LQQSYIMKRHGTEVDILGNIRESLPFLKKKQPS"
     gene            2800..3432
                     /gene="yihA"
                     /locus_tag="AC16A0_00520"
     CDS             2800..3432
                     /gene="yihA"
                     /locus_tag="AC16A0_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR3103837.1"
                     /translation="MTPDFTQDQIDAGRRLFGGPSTFVLGVAKLEQLPEVRGVEIAFA
                     GRSNVGKSSLINALTGVNGLARASNTPGRTRELNFFNVNDQLVLVDMPGFGFAKASKQ
                     EVKQWQSVLKGYLRGRPGLTRAFVLIDSRHGILAADREMFELLDESAVTYQLVLTKID
                     KIKPAELEKVAAATAKAIAKRPAAFPLIHLTSADKGTGIDALRAQIAGLI"
     gene            3498..>4269
                     /gene="argB"
                     /locus_tag="AC16A0_00525"
     CDS             3498..>4269
                     /gene="argB"
                     /locus_tag="AC16A0_00525"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR3103838.1"
                     /translation="MTEIPINETARILSEALPFLQRYDDKIIVVKYGGHAMIDPKLAQ
                     QFARDMVMLKVCGLNPIVVHGGGPQINKMLDKLDVKAEFRDGLRVTDAETMAVVEMVL
                     AGSINKSIVSSIQQAGGRAVGISGKDGNLMIAERLTKKKTDPATGETVEIDFGFVGEP
                     VRVNTEILETIMKSDAIPVIAPVGVGRNGDSFNVNADTAAGAIATATRAKRLLLLTDV
                     AGVLDKDKQLISELKIGDLPELIANGTITGGMIPKVESA"
BASE COUNT          815 a         1436 c         1294 g          724 t
ORIGIN      
        1 cgccaggcag cgacagggcc acaaccgcaa catagaccgc catgtagatc agcaccgaaa
       61 ggatcaggtg acgggcgacg aagtcctgga tctgcagctc atgcgccgca aaggactcga
      121 aactgagata gcgctgcagc ccggcgagat agaaggccag aagcgcaccg cccaggatca
      181 tgacgggaag ccagcgcttc agccgcgcgc gtgcggattt ggcggtgctt gcgctgaaat
      241 ggaccatgcc gcttcatgcg ccagtttgcc gtcccgcggt agggggcctc accggctcgt
      301 gacacatgtt caagccgcgt gcgtcaccgg gcgtcagcga cggcacggtg cgttgacttt
      361 ccggggcttt tcaccctata tgcccggcgc aattcctgaa gcggcagcaa ttcgtctcag
      421 ggcgtgcctg gcgggtcccc gcgatcccgg gacatgttgt tgaaatcatt ggccctgagc
      481 catttacgga gttctcactg tgaagcgcac ttaccagccg tccaaactcg tccgcaaacg
      541 ccgtcacggc ttccgggccc gcatggcgac cgtcggcggc cgcaatgtcc tggcccgccg
      601 ccgcgccaag ggccgcaagc gcctgtcggc ctaagggccg gatcacgaaa tccatggagc
      661 gcctcaagcg tcggcaggat ttcgttgccg ccgcccgggc gtcctatgcc gccatgccgg
      721 gcctgacggt acaggcccgc gaccgtggcg atgacggaag cccgcgggtc ggcttcaccg
      781 ccaccaagaa actcggcaac gccgtggtgc gcaatcgcgt caagcgccgg ctgcgcgaga
      841 ttgcgcgcca gcatctggca gcggcggccc tgccgggtca cgactacgtg ctgatcggcc
      901 ggcagcccgc cttcgcaagg gcctatgccg acctcgagaa agacttcacc tccgccctga
      961 agcggctgca ccatccgcgc cgtccggtcg agaaagaccc atcatgaaac acgacaacaa
     1021 gaacttcatc ctggccatcg tgctgtcgat ggcgatcatc ttcggatggc agtatttcta
     1081 cgccatgcct caggcggaca ggctggcgga acaggcccgg caggccgagc aggcaacggg
     1141 gcagcaaccc gcaaccccca ctgcgccgtc atccggcgcg ccggccggcc aggtgccggg
     1201 aaccgccggt gcaggtgtcg tcccgcgtga cgaggcactc gcctcgtcac ctcgggtgaa
     1261 gatcgacacc cccttcgtca gcggatcgat caacctcaag ggcgcgcagg tcgacgacct
     1321 gcatctgctg cgctatcgcg agaccatcga tcccaagagc ccgaccatca cgctgctgtc
     1381 gcctgccggt accgtgggcg ccaccttcgc cgagcagggc ttcattcccg caagcggcgt
     1441 gtccgccaag ctgccgacac ccgagacggt ctggacggcg ccgcccggag ccaccctgac
     1501 cgaaagcacc ccgctggtcc tgacctggga caatggcgaa ggcctggtct tcacccgcac
     1561 catcagcatc ggcgacgact atctcttttc ggtcacgcag agcgtcgaaa accgcggcgc
     1621 acagccggtc tcgctcttcc ctttcgcccg cgtcatccgc caggatacgc cggtggttgc
     1681 cggttactgg gtctttttcg agggcatgct cggcgttgcc gacggcgccc tgcaggaagt
     1741 ccactacagc gatgcatcgg aaaccaccga gccggtacgg gtcgacagta ccggcggctg
     1801 gctgggcttc accgacaagt actgggcagc cgtcgccatt cccgaccaga cccgcgccgt
     1861 gaccaccacc tatgcgcatg agaagctcgg tggccgcgat gtctaccaga ccaactacgt
     1921 cgcccgcgac gcgatgacgg ttgcgccggg tgccagcgct tcctaccagg accagcttta
     1981 tgccggggcc aaggtggtga gcatcatcaa caccatcggc gagcgctaca agatcgaccg
     2041 cttcgacctg atgatcgact ggggctggtt ccgcttcctg accaagccga tgttctatct
     2101 catcgactac ctgcagaagc tggtcggcaa cttcggcgtc gccattctgc tcgccacggt
     2161 tctggtgaag ctgctggtct tcccgcttgc caacaagtcc tatgcctcga tgagcaagat
     2221 gaagaagctg cagcccgaga tgctgaagct gaaggaactc tatcccgacg acaagatgaa
     2281 gcagcagcag gagatgatgg cgctgtacaa gcgggagaag gtaaacccga tgtcgggctg
     2341 ccttccggtg ctggtccaga tccccatttt cttcgcgctc tacaaggtga tcctgacctc
     2401 gatcgaattg cgccatgcgc ccttcttcgg ctggatccac gatctctcgg cgcccgatcc
     2461 gaccagcctg ttcaacctct tcggcctgat cccctggacg ccgccagcca tgctaatgat
     2521 cggcatctgg ccgatcatca tgggcatcac catgtggatc cagatgcgcc tcaaccctgc
     2581 gccgcccgat ccggtccagg ccagcctgtt caactggatg ccggtgttgt tcaccttcat
     2641 gctgggcacc ttcccggcgg gcctggtgat ctactgggcc tggagcaaca cgctgtcgat
     2701 cctgcagcag tcctacatca tgaaacggca tggcaccgag gtggatatcc tcggcaatat
     2761 ccgcgagagc ctgcccttcc tcaagaagaa gcagcccagt tgaccccgga cttcacacag
     2821 gaccagattg atgcgggccg ccgactcttc ggcggcccgt cgacattcgt gctgggcgtg
     2881 gccaagctgg aacaattgcc cgaggtccgc ggcgtcgaga tcgccttcgc cgggcggtcg
     2941 aacgtcggca agtcatcgct gatcaatgcg ctcacgggcg tcaatggact ggcgcgcgcc
     3001 tccaacacgc cggggcggac ccgcgagctc aacttcttca atgtgaacga ccagctggtg
     3061 ctggtcgaca tgccgggctt cggcttcgcc aaggcctcca agcaggaggt caagcagtgg
     3121 cagtcggttc tgaagggtta cctgcggggc cgtcccggtc tcacccgtgc cttcgtgctg
     3181 atcgactccc gccatggcat cctggctgcc gaccgcgaga tgttcgagct cctggacgaa
     3241 tccgcggtga cctaccagct ggtcctgacc aagatcgaca agatcaagcc cgccgagctg
     3301 gagaaggtgg ccgctgccac cgccaaggcg attgccaagc gcccggcggc ctttccgctg
     3361 atccatctga cctcggccga caagggcacg ggaatcgacg cactccgagc ccagatcgcg
     3421 ggcctcatct gaggcccttg gcagcggttg ccaatcaatt ataaggcgat ttccgcgcgc
     3481 ctggggaaca gccgacgatg accgagattc cgatcaacga gaccgcccgc atcctgtcgg
     3541 aagccctgcc cttcctgcaa cgctatgacg acaagatcat cgtggtgaag tacggcggcc
     3601 acgccatgat cgatcccaag ctggcccagc agttcgcccg cgacatggtg atgctgaagg
     3661 tctgcggcct caacccgatc gtggttcacg gcggcggtcc gcagatcaac aagatgctgg
     3721 acaagctgga cgtgaaggcc gagttccgcg acggcctgcg cgtgaccgat gccgagacca
     3781 tggccgtggt cgaaatggtc ctcgccggtt cgatcaacaa gtccatcgtg tcctcgatcc
     3841 agcaggcagg cggccgtgcc gtcggcatct ccggcaagga cggcaatctg atgatcgccg
     3901 aacgcctcac caagaagaag accgatccgg cgacaggaga gaccgtggag atcgacttcg
     3961 gcttcgtcgg cgagccggtg cgggtcaata ccgagatcct cgagaccatc atgaagagcg
     4021 atgccattcc ggtgatcgcg ccggtgggcg tgggccgcaa cggcgactcc ttcaacgtca
     4081 acgcggatac agcggccggc gccatcgcca ccgccacgcg ggccaagcgc ctgctgctgc
     4141 tgaccgatgt cgccggcgtg ctcgacaagg acaagcagtt gatcagcgag ctgaagatcg
     4201 gtgacctgcc tgagctcatc gccaacggca ccatcaccgg cggcatgatc ccgaaggtgg
     4261 aaagcgccg
//
LOCUS       DBNGVR010000024         3554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_8309, whole genome shotgun sequence.
ACCESSION   DBNGVR010000024 DBNGVR010000000
VERSION     DBNGVR010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_8309"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="AC16A0_00530"
     CDS             complement(<1..221)
                     /locus_tag="AC16A0_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3103839.1"
                     /translation="MTAVAIEKLDRIIHRFASVEHALSSGASGESFVKLSKEYSELEP
                     AARAAMGLKKAYGERRDVEDMLAAGGEMA"
     gene            complement(265..717)
                     /gene="dtd"
                     /locus_tag="AC16A0_00535"
     CDS             complement(265..717)
                     /gene="dtd"
                     /locus_tag="AC16A0_00535"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR3103840.1"
                     /translation="MRGLVQRVASASVAIDGCIAADIGRGFLVLVCAMAGDREADADT
                     LARKIAALRIFKDDAGKMNLPLAAVDGAVLVVSQFTLAADGMESGNRPGFSRAAPPAE
                     GERLYQRFCSALTSYGLRVETGVFGADMRVSLVNDGPVTIWFDTAKSP"
     gene            complement(728..1831)
                     /locus_tag="AC16A0_00540"
     CDS             complement(728..1831)
                     /locus_tag="AC16A0_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3103841.1"
                     /translation="MRDSTADQDRTSPAAIEYVREKPDGTLDPLGEAGWYLQHERETR
                     GETLDQAGEMTGIHPYHIEAIEYGDMTRMPERVEALEMIGTYAQYLGFDPEPLVQHYA
                     QFLPRPVIAPKANHPANPAPLSSAKILKFGKLPQMPKFAFKLNGIPGGAGGLVASLAG
                     AVMLFAGVSYMMMPDAADLAPSGQMAETRELPQIEPPTTPAEGETAEIKITDEPMTDD
                     ERVAAAAEYTADDLAGTGLDGLDDLISKSIPASKEQTASIGAVPSADDVAMTGDGREF
                     GIKNEDSRLTLKAKAPVWIRIEDARGNVVLTQMLNKGDTYKVPNREGLVVIARDGGLL
                     AYLIDGKEKGILGTPGEILVGRPLDLKTLGGKG"
     gene            complement(1932..>3554)
                     /gene="ptsP"
                     /locus_tag="AC16A0_00545"
     CDS             complement(1932..>3554)
                     /gene="ptsP"
                     /locus_tag="AC16A0_00545"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR3103842.1"
                     /translation="VPAERKRLEDAIHQLRTHVDELLDGSDAQRATEYSDVLETIRMF
                     AHDKGWVNRLREAIDTGLTAEAAVERVQNDNRARMMRTPDPYLRDRMHDLDDLSNRLL
                     RILTGAMATASRSDLPQNAIVVARNMGPAELLDYDRTKLRGVILEEGGKTSHVAIVAR
                     ALGIPAVGQAEGLIDLIDTGSPLIADGGTGEVFVRPTADLQKAYAEKVRFYAKKQAQY
                     AALRDVPAVSRDGVKIELNINAGLIVDLPHLHDSGAEGIGLYRTELQFMLAQRFPRLT
                     SQIRHYSTIIEQARGKPVTFRTLDIGADKILPYLRQPKEENPALGWRSIRMSLDRPAL
                     LRLQLRALMLAADGAPLKVMFPMIADVDEYKRAIEVVEIEKSYLLKRGHRLPQPLKLG
                     VMIEIPSLIWQLDHLLPQVDFASIGSNDLVQFLFASDRGNPKLAGRYDPLSPAALGAM
                     RQIVEKAEQHGKPVTLCGELGGRPLEAMGLIGIGLTSISMVPSAVGAVKAMVRTLDRG
                     RLWSFMEPLLRSPLHSIRPELLEFAQRNGVVL"
BASE COUNT          579 a         1142 c         1164 g          669 t
ORIGIN      
        1 tccgccatct cgcctccggc cgccagcatg tcctcgacgt cgcggcgctc gccataggcc
       61 ttcttcagcc ccatggcggc gcgcgccgcg ggctcgagct cggaatactc cttcgacagc
      121 ttcacgaagg attcgcctga tgcgccgctc gaaagcgcat gctcgacgga cgcgaaacga
      181 tggatgatgc gatccagttt ttcgatggca acggcagtca tgggcggcgc ttgtagccga
      241 tgcccggcgc tttcgcaaag tggctcacgg cgactttgcg gtatcgaacc aaatggtcac
      301 cgggccatca ttgaccaggc tcacccgcat gtccgcgccg aagactccgg tctcgacccg
      361 caatccgtaa ctggtcaggg cagaacagaa tcgctggtaa agccgttcgc cctcggctgg
      421 tggcgcggcc cgcgagaagc ccggccgatt gccactctcc atgccatcgg cggcgagcgt
      481 gaactggctg accacaagca cggcgccatc cacggcggcc agcggcaggt tcatcttgcc
      541 ggcatcgtcc ttgaagatgc gaagtgcggc gatcttgcgg gcgagggtgt cggcatcggc
      601 ctcgcggtcg cccgccatgg cacagaccag gaccagaaag ccgcggccga tgtcagccgc
      661 aatgcatcca tcgatcgcga cagatgcgct ggcgacgcgc tgaacaagtc cgcgcaagcg
      721 ctgccgatca gcccttgccg cccagggtct tgagatcgag cggccggccg accaggattt
      781 cgcccggcgt gccgaggatt cccttttcct tgccatcgat gaggtaggcg agcagtccgc
      841 catcacgcgc gatgaccacc aggccctcgc gattcggcac tttgtaggtg tcgcccttgt
      901 tcagcatctg ggtcagcacc acgttgccgc gggcgtcctc gatgcggatc cacacgggcg
      961 ccttggcctt cagcgtcagt cggctgtcct cgttcttgat gccgaattcg cgcccgtcgc
     1021 ccgtcatggc gacatcgtcg gccgagggca ctgccccgat ggaagccgtc tgttccttgg
     1081 acgccggaat cgacttgctg atcagatcgt caagcccgtc gaggccggtg cctgcgagat
     1141 cgtctgccgt gtattcggcg gctgccgcga cgcgctcgtc gtccgtcatc ggttcgtcgg
     1201 tgattttgat ctcggccgtc tcgccctcgg ccggcgtggt tggcggctcg atctggggca
     1261 gttcgcgcgt ctcggccatc tgtccggaag gcgcgaggtc ggctgcatcc ggcatcatca
     1321 tgtagctgac gccggcgaac agcatcaccg caccggccaa agatgcgaca aggccgccgg
     1381 cgccgccggg aattccgttc agcttgaagg cgaacttcgg catctgcggc agtttgccga
     1441 acttcaggat cttggcgctc gacagtggcg ccgggttggc gggatgattg gccttcggtg
     1501 cgatgaccgg gcgcggcagg aattgggcgt agtgctggac caggggttcc gggtcgaagc
     1561 ccaggtactg cgcataggtg ccgatcattt ccagggcttc gacacgctcg ggcatgcgag
     1621 tcatgtcacc gtattcgatg gcctcgatgt gatagggatg gatgccggtc atctcgccgg
     1681 cctgatccag cgtctcgccg cgtgtttcgc gctcgtgctg cagataccag ccggcttcgc
     1741 cgagaggatc cagggttccg tccggcttct cgcggacgta ctcgattgcg gccggactcg
     1801 tgcggtcctg atcggcggtg ctgtcacgca tggtgctact ctcggttacg ggtccgttaa
     1861 ccgtttgcta aggcaaactt tcccctaaaa tgaccgagaa tggtgaacgg cttgctaacg
     1921 cggacgcgcg ttcacagcac cacgccgttg cgctgggcga attccagcag ttccggacgg
     1981 atcgagtgca gggggctacg cagcagtggc tccatgaagc tccacaatct gccgcggtcg
     2041 agagttcgca ccatggcctt caccgcgccc acggcagagg gcaccatcga gattgacgtc
     2101 aggccgatgc cgatgaggcc catcgcttcc agcggccgtc cgcccagttc gccgcacaag
     2161 gtcaccggct tgccatgctg ttcggccttc tcgacgatct gccgcatggc gccaagcgca
     2221 gccgggctca gcgggtcata gcggcccgcg agcttgggat tgccgcggtc ggacgcgaag
     2281 aggaactgca ccagatcgtt cgacccgatg gaggcgaagt cgacctgcgg cagcagatgg
     2341 tccagctgcc agatgagcga tgggatctcg atcatcacgc ccagcttcag cggctggggc
     2401 aacctgtgtc cgcgcttcag cagataggac ttctcgatct cgaccacctc gattgcccgc
     2461 ttgtactcat cgacatcggc gatcatcggg aacatgacct tgagcggcgc gccatcggcc
     2521 gccagcatca gggcgcgcag ctgcagccgc agcagggccg ggcggtccag cgacatgcgg
     2581 atcgagcgcc agccgagcgc ggggttctct tccttgggct ggcgcaggta gggcaggatc
     2641 ttgtcggcgc cgatatcgag tgtgcggaag gttaccggct ttccgcgcgc ctgctcgatg
     2701 atggtggaat agtgtcggat ctgcgaagtc agccgcggga agcgttgggc cagcatgaac
     2761 tgcagctcgg tgcggtagag tccgatgccc tccgctccgg aatcatgcag atgcggcagg
     2821 tcgacgatca ggccggcatt gatgttgagc tcgatcttga cgccgtctct cgagacggcg
     2881 ggaacatcgc gcaacgccgc atactgggcc tgcttcttgg catagaagcg aaccttctcg
     2941 gcataggcct tctgcaggtc ggcggtgggc cgcacgaaga cctcgcccgt tccgccatcg
     3001 gcgatgagcg ggcttccggt atcgatcaga tcgatgagcc cttccgcctg gcccacggcg
     3061 ggaatgccca gcgcccgcgc cacgatggcg acatggctgg tcttgccgcc ttcctcgaga
     3121 atcacgcccc gcagcttggt gcggtcatag tcgaggagtt ccgccggacc catgttgcgc
     3181 gccaccacga tcgcgttctg cggaaggtcg ctgcgcgagg ccgtggccat ggcgccggtc
     3241 aggatgcgca gcagccggtt cgacaggtcg tcgagatcgt gcatgcggtc gcggagatag
     3301 ggatcgggcg tgcgcatcat gcgggcgcga ttgtcgttct gcacgcgctc caccgcggcc
     3361 tcggcggtga ggccggtgtc gatcgcctcg cgcagccggt tcacccagcc cttgtcatgg
     3421 gcgaacatac ggatggtttc gagcacatcc gagtactcgg tggcgcgttg tgcatccgac
     3481 ccgtcgagca gctcgtccac atgggtgcgc agctgatgaa tggcatcttc cagccgcttg
     3541 cgctcggcgg gcac
//
LOCUS       DBNGVR010000025         3987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2955910, whole genome shotgun sequence.
ACCESSION   DBNGVR010000025 DBNGVR010000000
VERSION     DBNGVR010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2955910"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..905)
                     /locus_tag="AC16A0_00550"
     CDS             complement(60..905)
                     /locus_tag="AC16A0_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3103843.1"
                     /translation="MKLLRYGPKGKEKPGLLDKDGKIRDLSRVVPDISGDVIGPKGLA
                     RLRKIKPESLPLVKGNPRIGACIANPQKFIAIGLNYSDHAAESGLKVPPEPVVFTKQV
                     NCVSGPYDDVTIPPKSKKSDWEVELGVIIGSVTKNIKKKDAMAHVAGYCTINDLSERE
                     FQAERSGQWTKGKSYDTFGPVGPWLVTADEVKDPQKLHMWLELNGKRVQDGSTATMVY
                     GVAFIVAYLSQFFTLMPGDIITTGTPPGVGMGMKPQRFLKPGDTMRLGIDGLGEQFQK
                     VYRDK"
     gene            complement(902..1456)
                     /locus_tag="AC16A0_00555"
     CDS             complement(902..1456)
                     /locus_tag="AC16A0_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103844.1"
                     /translation="MLHAVPQRGTMLGNRGGRFHRDDRTLGKRRWASRHWICCELHYK
                     GMHHEAMGRGYTSLFFLDEVTALSAGHRPCFFCRRTEATAFLGRQKVDGFDRALHAER
                     LGPKPDIDLDVLPDGAMVEIDGTAFALRGGNLLRWSFDGYRDFRPRRASMRGRLLTPP
                     SIAAILRAGYSPRWHESARQGDGT"
     gene            complement(1504..2169)
                     /locus_tag="AC16A0_00560"
     CDS             complement(1504..2169)
                     /locus_tag="AC16A0_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3103845.1"
                     /translation="MSRDAILAKIRARTSKGDEETRRMIVDEHLAEHRRNLIPVRGQG
                     DDDHRIGVFTRMMEAVGGTVELLDDVNDVPHSVAAYLRNGNLPAHIRRGADPLLAKLP
                     WHRGGAIEVEEGRAAETDRASITRAFAGVAESGTIIQVSGPDNPTTLNFLPEAHIVVL
                     ELDSLFASYEEAWSKLRKAFGEGVMPRTVNMISGPSRTADIEQTIVRPAHGPKNMHVI
                     IVR"
     gene            complement(2166..3581)
                     /locus_tag="AC16A0_00565"
     CDS             complement(2166..3581)
                     /locus_tag="AC16A0_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR3103846.1"
                     /translation="MDASTATFPQNVKRALADSQLQHAMTETGPRFISKRSKAREALP
                     EFDALRDQARDIKDHVLEFLDLYLERYEEKVVAAGGKVHWAATAEEARAAILGICQRV
                     KARTVTKGKSMISEEIDLNHHFEANGITPVETDLGEYLIQLRKERPSHIIAPAVHLTR
                     DQVEADFRKAHTHLDPKRDLTEIPALVEEARQVLRQKYFDADVGVTGANFLVAETGTS
                     IIVTNEGNGDLTQILPKVHVVIASIEKIVPTLEDAATSLRVLARSATGQDFSNYTTLS
                     TGPKRPDDLDGPEEYHVVILDNGRSDVLGTEFQDMLRCIRCGACLNHCPVYQAVGGHA
                     YGWVYPGPIGSVVTPALVGIEKAKHLPNASTFCGRCEEVCPMRIPLPRMLRSWREKEF
                     EKHLQPQAMRAGLGLWAFLAKRPKLYQMVTRLAARVLHNRGHGTGRISSLPLASGWTQ
                     CRDFPAPQGKTFQQMWKERRK"
     gene            complement(3581..>3987)
                     /locus_tag="AC16A0_00570"
     CDS             complement(3581..>3987)
                     /locus_tag="AC16A0_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3103847.1"
                     /translation="TRVMKVTAVDAKFPAKATYHDSCSGLRELGIRNEPRQLLASVEG
                     VTLKEMTDAEVCCGFGGTFCVKYPGISDKMLEKKLKHIAATGADTLIAGDLGCLMNMA
                     GKLKREGSHIRVRHVAEVLAGDAATPSIAEAE"
BASE COUNT          638 a         1290 c         1311 g          748 t
ORIGIN      
        1 tggcgtatgg aggctgggga tcaggttaac gccccactcg ccattcgcca ctcgcctccc
       61 tacttgtccc gatagacctt ctggaactgc tcgcccaggc cgtcgatgcc cagccgcatg
      121 gtgtcgcccg gcttgaggaa gcgctgcggc ttcatgccca tgccgacacc cggcggcgtg
      181 ccggtggtga tgatgtcgcc cggcatgagg gtgaagaact gcgagagata ggcgacgatg
      241 aaggccacgc catagaccat ggtggcggtc gagccgtcct gtacgcgctt gccgttcagc
      301 tccagccaca tgtgaagctt ctgcgggtcc ttgacctcgt ccgccgtcac cagccagggg
      361 ccgaccgggc cgaaggtgtc ataggacttg cccttggtcc attgcccgga ccgctcggcc
      421 tggaactcgc gctccgacag gtcgttgatg gtgcaatagc ccgcgacatg ggccatggca
      481 tccttcttct tgatgttctt ggtgacgctg ccgatgatca cgcccagttc aacctcccag
      541 tccgacttct tggacttggg cgggatggtg acgtcgtcat aaggcccgga aacgcagttc
      601 acctgcttgg tgaagaccac cggctccggc ggcaccttga gtcccgattc cgcggcgtgg
      661 tcggagtagt tcaggccgat ggcgatgaac ttctgcggat ttgcgataca ggcgccgatg
      721 cgcggattgc ccttcaccag cggcagcgac tccggcttga tcttgcgcag ccgcgccagt
      781 cccttcggtc cgatcacgtc cccgctgatg tcgggcacca cgcgcgacag gtcgcggatc
      841 ttgccgtcct tgtcgagcag gccgggcttt tccttgccct tcgggccgta acgcagaagc
      901 ttcatgtgcc atccccttgt cttgccgact cgtgccagcg cggtgaatat ccggcgcgca
      961 aaatggccgc gatcgacggc ggtgtcaaaa gccttccgcg catggaggcc ctgcggggac
     1021 gaaaatcgcg atagccgtcg aaggaccagc gcagcaggtt gccaccgcgc agcgcgaagg
     1081 cggtgccgtc gatctccacc atcgcgccat ccgggaggac atcgagatca atgtcaggct
     1141 ttggaccaag ccgttcggca tgcagggcgc ggtcgaatcc atcgaccttc tggcggccga
     1201 gaaaggcggt ggcctcggtc cgccggcaga agaagcaagg ccggtggccg gcggaaagtg
     1261 cggtcacctc gtcgagaaag aagagcgacg tatagcccct tcccatggcc tcgtgatgca
     1321 tgcccttgta atgcagctcg cagcagatcc agtgccggct ggcccagcgt cgttttccca
     1381 gcgtgcggtc gtcgcgatgg aagcgtccgc cgcggttgcc cagcatggtg ccgcgctgcg
     1441 gcacggcatg cagcacgccc caggatcgac gcggttctgc aggctcacgc cgcggcgtcc
     1501 gcgtcagcgg acgatgatga catgcatgtt cttggggcca tgggcgggcc tcacgatggt
     1561 ctgctcgatg tcggcggtgc gcgaggggcc ggagatcatg ttgaccgtgc gcggcatgac
     1621 gccttcgccg aaggccttgc gcagcttgct ccaggcctct tcgtagctgg cgaagaggct
     1681 gtccagttcc agcacgacga tatgcgcttc gggcaggaag ttcagcgtcg tcgggttgtc
     1741 ggggcctgag acctggatga tggtgccgga ctccgcaacg ccggcgaagg cgcgggtgat
     1801 ggaggcgcga tccgtctccg ccgcacggcc ctcttcgacc tcgatcgcgc cgccgcgatg
     1861 ccagggcagt ttcgccagca gcggatcggc gccgcgccgg atatgggccg gaagattgcc
     1921 attgcgcaaa taggccgcga cggaatgcgg cacgtcgttc acatcgtcca gaagctccac
     1981 cgtgccgccg acggcttcca tcatccgggt gaagacgccg atgcggtgat cgtcgtcgcc
     2041 ctgcccgcgt accgggatga ggttgcggcg atgctcggcc agatgctcgt ccacgatcat
     2101 gcgccgggtt tcctcgtcgc ccttggaggt gcgcgcgcgg atcttcgcga ggattgcgtc
     2161 gcggctcatt ttcgccgctc cttccacatc tgctggaagg tcttgccctg cggcgccggg
     2221 aagtcgcggc attgcgtcca gccggatgcc agcggcaggc tggagatgcg gccggtgccg
     2281 tggccccggt tgtgcagcac gcgggcggca aggcgcgtga ccatctgata cagcttcggg
     2341 cgcttggcga ggaaggccca gagcccgagg cccgcacgca tggcctgcgg ctgcaggtgc
     2401 ttctcgaatt ccttctcgcg ccacgaacgc agcatcctgg gcaagggaat ccgcatgggg
     2461 cagacttcct cgcaacgccc gcagaaggtg gacgcattgg gcaggtgctt ggccttctcg
     2521 atgccgacca gcgccggcgt caccaccgag ccgatgggac cgggatagac ccagccatag
     2581 gcgtggccgc ccaccgcctg gtagaccggg cagtggttga gacaggcacc gcagcggatg
     2641 caacgcagca tgtcctggaa ctcggtgccc agcacgtcgg agcggccatt gtcgaggatg
     2701 acaacgtggt actcctccgg gccgtcgagg tcgtcaggcc gcttcggccc ggtggacagc
     2761 gtcgtgtagt tggagaagtc ctgtcccgtt gccgagcgcg ccagcacgcg cagactggtg
     2821 gccgcgtctt ccagcgtcgg cacgatcttc tcgatcgatg cgatcaccac atggaccttg
     2881 ggcaggatct gggtcagatc gccattgccc tcgttggtga cgatgatcga ggtgccggtc
     2941 tcggccacca ggaaattggc gccggtcacg ccgacatcgg catcgaagta cttctggcgc
     3001 agcacctgcc gcgcctcctc gaccagcgcg gggatttcgg tcaggtcgcg cttggggtcg
     3061 agatgcgtat gcgccttgcg gaagtcagct tccacctggt cgcgcgtcag atgcacggcc
     3121 ggcgcgatga tgtggctggg ccgttccttg cggagctgga tcagatactc gccgagatcc
     3181 gtctcgaccg gcgtgatgcc attggcttcg aagtgatggt tgaggtcgat ctcctcggag
     3241 atcatcgact tgcccttggt caccgtgcgg gccttgaccc gctggcagat gcccaggatc
     3301 gccgcgcggg cctcttccgc cgtcgcggcc caatgcacct tgccgccggc ggccacgacc
     3361 ttctcctcgt atcgctcaag atagaggtcg agaaattcga gaacgtgatc cttgatgtcg
     3421 cgtgcctggt cgcgcaaagc gtcgaattcg ggaagggcct cgcgcgcctt gctgcgcttg
     3481 gagatgaagc gcgggcccgt ctcggtcatg gcatgctgca gctggctgtc ggccagcgcg
     3541 cgcttcacgt tctgcggaaa ggtcgcggtt gaagcgtcca ttactccgcc tccgcaatcg
     3601 acggcgttgc ggcatcgccc gccagcactt ccgccacatg ccgcacgcgg atgtgcgagc
     3661 cttcgcgctt cagcttgccc gccatgttca tgagacaccc cagatcgccc gcgatcagcg
     3721 tgtcggcgcc ggttgcggcg atgtgcttca gtttcttctc cagcatcttg tccgagatgc
     3781 cgggatactt cacgcagaag gtaccgccga agccgcagca gacctcggcg tcggtcattt
     3841 ccttcaacgt cacgccctcg acgctggcca gcaactggcg cggctcgttg cgaatgccaa
     3901 gctcgcgcag gcccgagcag gagtcatgat aggtggcctt ggccgggaac ttcgcatcga
     3961 ccgccgtcac cttcatcacc cgcgtga
//
LOCUS       DBNGVR010000026         2496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1580152, whole genome shotgun sequence.
ACCESSION   DBNGVR010000026 DBNGVR010000000
VERSION     DBNGVR010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1580152"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16A0_00575"
     CDS             <1..410
                     /locus_tag="AC16A0_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HIR3103848.1"
                     /translation="IFPTLAKLSGGSTARSKPLDGVDVWPSLSSGAPSPRSEIVYNVE
                     PFRAAIRQGDWKLVWSSLLPPKVELFDLGKDPGEANNLAAANPQRVQELQARVLELAG
                     QMSPPLFLMELVSLGLSVAPVFPDGDMSLVNQD"
     gene            complement(416..1279)
                     /locus_tag="AC16A0_00580"
     CDS             complement(416..1279)
                     /locus_tag="AC16A0_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3103849.1"
                     /translation="MAAVLLGVFAALCWSLHDLVARSYAERIGPFRMAAVVMVAGAAM
                     LLGVVLWRGTIWTASTEGLTEGLLLGLAYGLGVGGLFKAFSLGPISLVGPVVGGYPIL
                     VVFWGVAHGLIPTPLQWTAVAATLVGAVIVARSGPADGGINAVVPGKLPQLALFGVIS
                     ALGYSASIVLGQNAAVSVGEIEATFLSRFTALVTILPFLLGEPRPKRLAGAHWLGMLA
                     MALLDVFGVIAVNASGHLPGKEFAAIGISSYGAIAVVLAMLILKEKVSAGQWLGILMI
                     AAGVATLSLTS"
     gene            1442..>2496
                     /locus_tag="AC16A0_00585"
     CDS             1442..>2496
                     /locus_tag="AC16A0_00585"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HIR3103850.1"
                     /translation="MFLSVFELFKIGIGPSSSHTVGPMVAARRFLALAAAASLPDTGR
                     LSVSLHGSLAFTGKGHGTDRAVVLGLAGEAPDRIDPDRVDAVLAAMTVARRISLSNGQ
                     TLSFDPARDVIFDFGPALPGHSNGMSFRILDDDNNTLLAETYYSIGGGFVQTEAERAA
                     AVAEGKQARPSLDVPYPFRNAAEMLHMGKVAGLTIAQMKRVNETRHMSDAELDAGLDR
                     IWNAMNACMSRGLSLSGTLPGGLKIKRRAAEIFAKLKDEQGGNSIQPHRVMDFLSVYA
                     MAVNEENAAGGRVVTAPTNGAAGVMPATIRYYLDHCIGADPAGIRTFLLAASAVGGII
                     KHNASISGAEVGCQGEVG"
BASE COUNT          438 a          867 c          797 g          394 t
ORIGIN      
        1 acatcttccc gaccctggcg aagctctcgg gcggctcgac ggccagaagc aagccattgg
       61 acggcgtcga cgtctggccc agcttgagca gcggcgcgcc atcgccacgc agcgagatcg
      121 tctataacgt cgagcccttc cgcgccgcta tccggcaggg cgactggaag ctggtgtgga
      181 gttcacttct gccgccgaag gtcgagctct tcgacctggg caaggatccg ggcgaagcaa
      241 acaatcttgc cgccgccaat ccgcagcgcg tgcaggagct gcaggcgcgg gtgttggagc
      301 tggccggcca gatgtccccg ccgctgttcc tgatggaact cgtcagcctc ggcctctccg
      361 tcgcaccggt attccctgat ggcgacatga gcctggtcaa ccaggattag cgactctaac
      421 tcgtcagcga cagcgtcgcc acgccggcgg cgatcatcag gatgcccagc cactgcccgg
      481 ccgagacctt ttccttgagg atcagcatgg ccagaacgac ggcgatggct ccgtaggacg
      541 agatgccgat ggccgcgaac tccttgcccg gcagatgtcc ggaggcattg accgcgatca
      601 cgccaaagac gtcgagcagc gccatggcca gcatgcccag ccaatgtgcc cctgcgagcc
      661 gtttcggccg cggttcgccg agcaggaagg gcaggatggt caccagcgcg gtgaagcgcg
      721 acaggaaggt ggcctcgatc tcgcccaccg acacggcggc gttctggccc agtacgatgg
      781 aggcggaata gcccagcgcc gagatcacgc cgaacagcgc gagttgaggc agcttgccgg
      841 gcaccaccgc attgatgccg ccgtccgccg ggccgctgcg cgccacgatg actgcgccaa
      901 cgagggttgc cgccaccgcc gtccattgca gcggcgtggg gatcagccca tgcgccacgc
      961 cccagaagac gacgaggatc ggatagccgc ccaccaccgg gcccaccagc gaaatcggcc
     1021 ccagcgagaa ggccttgaaa agcccaccca cgcccagccc gtaggcaagg cccagcagca
     1081 ggccctcggt cagcccctcg gtcgaggccg tccagatggt gccgcgccac agcacgacgc
     1141 ccagcagcat ggcggcgccc gccaccatga ccacggcggc catgcggaac ggcccgatgc
     1201 gttcggcata gctgcgcgcc accaggtcat gcagcgacca gcacagggcg gcgaaaacgc
     1261 cgagaagaac cgccgccatg atcggtgaat cgctccgtga tctgtttccg gcggatcaaa
     1321 gcatagctgg cgtggccgat caaagcagcc cggtccgcca ctggcggcgc agccgttcct
     1381 ggtcgataat gcaggctgtt cggccgtgcc gctcgggctc ggccctgatg gattgggcct
     1441 catgtttctt tcggttttcg agctcttcaa gatcggcatc ggaccttcca gttcgcacac
     1501 cgtgggtccc atggtggcgg cgcggcggtt tctcgcgctg gccgcggccg cgtcgctgcc
     1561 cgacacgggc cgcctgagcg tcagcctgca tggctcgctc gccttcaccg gcaagggcca
     1621 cggcaccgat cgcgccgtcg tcctcggcct tgccggcgaa gcgcccgaca ggatcgatcc
     1681 cgaccgcgtg gatgccgtgc ttgccgccat gacggtggcc cgccgcatca gcctgtccaa
     1741 tggccagacg ctgagcttcg atcccgcccg cgatgtcatc ttcgatttcg gcccggcctt
     1801 gcctggccat tccaacggca tgtccttccg catcctcgat gacgacaaca acacgctgct
     1861 ggccgaaacc tactactcca tcggcggcgg tttcgtgcag accgaagccg agcgtgccgc
     1921 tgcggtggcc gaaggcaagc aggcgcggcc ctcgctcgac gtgccctatc ccttccgcaa
     1981 cgctgcggag atgcttcaca tggggaaggt cgccggcctc accatcgcgc agatgaagcg
     2041 cgtcaacgaa acccggcaca tgagcgatgc cgaactcgac gccggtctcg atcgcatctg
     2101 gaacgccatg aatgcctgca tgtcgcgcgg cctttcgctc agcgggacgc ttccgggcgg
     2161 attgaagatc aagcgccgcg ccgccgagat cttcgccaag ctcaaggacg agcagggcgg
     2221 gaactcgatc cagccgcatc gcgtgatgga tttcctttcc gtctatgcca tggcggtcaa
     2281 cgaggagaat gcggcagggg gcagggtggt gaccgcgccc accaatgggg ctgcaggcgt
     2341 catgccggcc accatccgct actatctcga tcattgcatc ggcgccgatc ctgccgggat
     2401 ccgcaccttc ctgctggcgg cttcggccgt cggcggcatc atcaagcaca atgcctcgat
     2461 ctcgggtgcc gaggtcggct gccagggcga ggtggg
//
LOCUS       DBNGVR010000027         2783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_992146, whole genome shotgun sequence.
ACCESSION   DBNGVR010000027 DBNGVR010000000
VERSION     DBNGVR010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_992146"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC16A0_00590"
     CDS             <1..612
                     /locus_tag="AC16A0_00590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3103851.1"
                     /translation="MDRSMKLPRAMDAMFNRALATDDIKVRPLDKKNLERDLRIIIDI
                     FNDAWSENWGFVPFTPEEITKLGNDLKMLVKGDYISIATYQGEPVAMAVTLPNINDWI
                     FDLKGRLLPFGLPKLLWRMLSRHPRSVRMPLMGVKKAYHGTAIGSALALTVIETLRRY
                     HVARGTEQAELSWILEDNMPMRRMIESLGARPYKTYRVFEKDL"
     gene            612..1382
                     /locus_tag="AC16A0_00595"
     CDS             612..1382
                     /locus_tag="AC16A0_00595"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3103852.1"
                     /translation="MAKPWNALILAAGRGPDDPMAKAYGVANKCTIPIAGKPMLLRVA
                     EALETSGVIGPIAVSIEDPGIVSALLGDRALALTPAHSAPASVMAALGSGKLTYPVLV
                     TTGDHALLTPDMVRHFCAESAAIDADFTAGLATAETILAAYPDSIRTYFTLGPDRVSG
                     CNLFAVKNARALSLLDRWQYLEQVRKKPWRLIAAFGISPLLRFLTGRLDLEQAFQIVS
                     ARLGLLARPVVMPFAEAAIDVDKPADKELAERIAARRG"
     gene            complement(1383..2114)
                     /locus_tag="AC16A0_00600"
     CDS             complement(1383..2114)
                     /locus_tag="AC16A0_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIR3103853.1"
                     /translation="MRVIILAAGQGKRLLPLTAEVPKALLDIHGRTLIARQIDAFAAC
                     GISDFVVVTGYASARIEAALAEIARSLKVGIRTVYNPFYAVADNLASCWMARGEMTTD
                     FIQVNGDNVFRADLVERLLSAEPRPVTVAINRKDAYDPDDMKVMLDQGRLTEIGKTLP
                     VDTTDAEAIGFYIFRGDGVKAYAKQLEMAMREPSGLKQWFPSAVGSLAKQLDINTIAI
                     TGLRWCEVDFPVDLQQARQLVASWG"
     gene            2188..>2783
                     /locus_tag="AC16A0_00605"
     CDS             2188..>2783
                     /locus_tag="AC16A0_00605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3103854.1"
                     /translation="MFSLAHLSDLHLGPLPEGAARRHFALKRSIGAANWRLRRHKQHD
                     PAVAAALAMDIRAANPDHVACTGDIVNVAAHAEFPAAAAWLTDFGPPDWISFVPGNHD
                     TYVRLSWENGLVHLAPYMDGDMKVRLTQTSHQIATPFPYVRLRRNVALIGLCSGEPQN
                     LFKAAGRLGETQLESLAILLRDLRERGYARVLMIHHPPL"
BASE COUNT          497 a          926 c          861 g          499 t
ORIGIN      
        1 atggaccgca gcatgaagct gccgcgggcg atggacgcca tgttcaatcg ggccctggcg
       61 acggacgaca tcaaggtgcg gccgctcgac aagaagaacc tcgaacggga cctgcggatc
      121 atcatcgaca tcttcaacga tgcctggagc gaaaactggg gctttgtgcc ctttacgccg
      181 gaagagatca ccaagctcgg caatgacctc aaaatgctgg tgaagggcga ctacatctca
      241 attgccacct atcagggcga acccgtggcc atggccgtga ccctgcccaa catcaatgac
      301 tggatcttcg acctcaaggg ccggctactg ccgttcgggc ttccgaagct gttgtggcgc
      361 atgctgtccc ggcatccgcg ttcggtccgc atgccgctga tgggggtcaa gaaggcctat
      421 cacggcacag ccatcgggtc ggcccttgca ctgacggtca tcgaaacgct gcgccgctat
      481 cacgtggccc gcggcactga gcaggccgaa ctgtcatgga tcctcgagga caacatgccc
      541 atgcgccgca tgatcgagtc gctgggggca aggccctaca agacctaccg cgtcttcgag
      601 aaggacctgt gatggcgaag ccctggaatg ccctgatcct tgcggcaggg cgcggtcccg
      661 acgacccgat ggcgaaggcc tatggcgtgg ccaacaaatg caccatcccc attgccggca
      721 agcccatgct gctgcgcgtc gccgaggcac tcgagacgtc tggcgtgatc ggacccattg
      781 ctgtctcgat cgaagatccc ggaatcgtgt cggccctgct cggggacagg gcgctcgcgc
      841 ttacccctgc ccacagcgcg cccgcctccg tgatggcagc cctgggctcc ggcaagctga
      901 cctatccggt gctggtcacc accggcgacc atgccctgct gacgccggac atggttcgcc
      961 acttctgcgc cgagtcagca gccatcgatg ccgacttcac ggcgggactg gccacggccg
     1021 agaccattct cgccgcctat ccggactcga tccgaaccta cttcaccctg gggccggacc
     1081 gtgtttcggg ctgcaatctc ttcgccgtga agaatgcgcg tgcgctgagc cttctcgatc
     1141 gctggcagta cctcgaacag gtgcgaaaga aaccatggcg cctgatcgcc gccttcggaa
     1201 tctctccgct cttgcgcttc ctgaccggcc gtcttgacct cgaacaggcc tttcagatcg
     1261 tctctgcgcg gcttggcctg ctggcacggc cggtggtgat gcctttcgcg gaggcggcga
     1321 tcgatgtcga caagcccgca gacaaggaac tggccgagcg cattgccgcc aggcgcggct
     1381 gatcagcccc agctcgccac cagctgccgg gcctgctgca ggtccaccgg gaagtccacc
     1441 tcgcaccagc gcaggccggt gatcgcgatg gtattgatgt cgagttgctt ggccagcgat
     1501 cccaccgcgg aggggaacca ttgcttcagg cccgagggct cgcgcatcgc catctcgagc
     1561 tgtttcgcat aggccttcac cccgtcgccg cggaagatgt agaagccgat ggcctcggca
     1621 tccgtcgtgt cgaccggcag ggtcttgccg atctcggtga gtcggccctg gtcgagcatg
     1681 accttcatgt catcagggtc gtaggcatct ttgcgattga tggcaacggt caccggccgc
     1741 ggctccgccg agaggaggcg ctccaccaga tcggcgcgga agacgttgtc gccattgacc
     1801 tggatgaagt cggtggtcat ttcgccccgc gccatccagc agctcgccag attgtcggcg
     1861 accgcataga agggattgta gacggtgcga atgccgacct tgaggctgcg ggcgatctcc
     1921 gccaaggcgg cttcgatccg ggccgaggca tagccggtca ccaccacgaa atcactgatg
     1981 ccgcaggcgg caaaggcatc gatctggcgg gcaatgaggg ttcggccgtg aatgtccagc
     2041 agggccttgg gcacctcggc ggtgagcggc aggagccgct tgccttggcc tgcggcaaga
     2101 atgatgactc gcacgatgct tgaaccctga tattgcggac agctagaagg ccgtttcaat
     2161 cgcactcgat tcccgggaag tcaacgcgtg ttcagtctcg cccacctgtc cgacctgcac
     2221 ctggggccgc tgcccgaggg tgcggcccgg cggcatttcg cgctcaagcg ctcgatcggc
     2281 gcggcgaact ggcggctccg tcggcacaag cagcatgatc ccgccgtggc ggcagccttg
     2341 gccatggaca tccgggcggc caatccggac catgtcgcgt gcaccgggga catcgtgaat
     2401 gtggccgccc atgccgaatt tccagccgcc gccgcctggc tcacggattt cggaccgccg
     2461 gactggatca gtttcgttcc gggcaaccac gacacctatg tgcggctcag ctgggaaaat
     2521 ggcctggtcc acctggcgcc ctacatggat ggcgacatga aggtccgact cacacagaca
     2581 agccaccaga tcgccacacc ctttccctac gtccgcctgc gccgcaatgt ggcgctcatc
     2641 ggcctgtgtt ccggcgagcc gcaaaatctc ttcaaagccg ccgggcggct gggcgagacc
     2701 cagcttgaat cgctcgccat cctgctgcgc gatctgcgcg aacgcggcta cgcccgggtg
     2761 ctgatgatcc atcatccgcc cct
//
LOCUS       DBNGVR010000028         3243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4134895, whole genome shotgun sequence.
ACCESSION   DBNGVR010000028 DBNGVR010000000
VERSION     DBNGVR010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4134895"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16A0_00610"
     CDS             <1..479
                     /locus_tag="AC16A0_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103855.1"
                     /translation="IPHPVDSTSGTCEVSIELLNKSTHIAHQPFICLTDLGLQLTPAK
                     GWSMDEIVSIRRMRRFSARTGISRLEPLAHVHCCTISLLFSTTGGGFVEYEVGNWHPF
                     ETLPDLRISCVVGAGNYPSERLPITVKAESLREFMRELTERGEVPAPEATEIAAAQ"
     gene            complement(473..>3243)
                     /locus_tag="AC16A0_00615"
     CDS             complement(473..>3243)
                     /locus_tag="AC16A0_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR3103856.1"
                     /translation="VALGGSGTIETATGSFDLRIQGKADSPQTGISAVDRLLAGPVTA
                     EGRITGGNGDGIGLDGLTLTSAALTASASGHASAAAADLALAVRLPDLALVHEGLGGT
                     AQLDAGLKGPWQGLTARINGRGEAVTLMGKSLERPVAAAEVKIENAAPSGTLTASGRL
                     DGKPVDIAASFASDAAGDVVLENLTAIAGSARLSGKLRWPKGDAPTGTLGFSAPDLAE
                     VAPLFLTELAGAATGDIEMRGAGEAHATEIRFNGRNIVTPSFTAATAEGNISIATLFG
                     VPRPEGQVKLGKARIGGQRFDSATVTAKATGTNTYRTAVKLAGPDLSLDADAVVTLGS
                     GEARYAVSRLAGKLRGIPFSASAPLVLQQAGETVTLEPARLAIGKGSIRVGGRLLPDL
                     KATVTVQSLPLAAFAGLGGQPGLQGTLSGEAVLTGSLSAPEGSFKLSGAGLSTETLKQ
                     LGVRPMSASANGTISGGTLRATLKADAGKQLSVTGNGTVDLRTSRIDMELKGRTGPAL
                     FADRLARSGVRAQGDVGFDLRLSGALDRPDINGQITLRKGVLGDAAGRFTLRDAAGSA
                     DVSNRVVRITSLSGVTGRNGRATVIGTIALDAGLTASLDARVENGMYTDGSFVTARYD
                     ARLKITGPLLRSPLATGEIDLRGTKITLSELPKRALTPDDVKHINASRAVALQAREIR
                     RNRPTESSSLRVDVRLRALDEVSVSGRGLNVILNGGLRIYGPLDNLAADGSFQMVRGR
                     LTLPARSLDFERGSLTFDRDLDPKIDFVAVSRRKDATITLTVSGYASEPSIQVTSVPQ
                     MPQEEAMARLIFDNSMLELSPLQIAQIASYVATLSGGGDSGLLSGLQNALGLDQLSIT
                     ENESGGTDVGIAKRLNDKLSIGVEQTTQSNTTRVIIDLSATPELKVRGSIDSEGSSRI
                     GVYYEKDY"
BASE COUNT          529 a         1083 c         1061 g          570 t
ORIGIN      
        1 tcatccccca tcccgtggac agcaccagcg gcacctgcga agtcagcatc gagctgctga
       61 acaagtcaac ccacatcgcc caccagccat tcatctgcct gaccgatctc ggcctgcagc
      121 tcactccggc caagggctgg tccatggacg agatcgtttc gatccgccgc atgcggcgat
      181 tctcggcacg taccggcatc tcacgcctcg aaccgctggc gcacgtacat tgctgcacga
      241 tctccctgct cttctcgacg accggcggcg gcttcgtgga gtacgaagtc ggcaattggc
      301 atcccttcga gaccttgccg gatctgcgga tcagctgcgt cgtgggtgcc ggaaactatc
      361 cttccgaacg cctgccgatc accgtgaagg ccgagtccct gcgcgagttc atgcgggagc
      421 tgaccgagcg cggcgaagtg cccgcaccgg aagcgaccga gattgcggcg gctcagtaat
      481 ccttctcgta gtagacgccg atgcgcgacg aaccttcgga gtcgatcgac cccctcacct
      541 tgagttccgg cgtcgccgac agatcgatga tgacccgcgt ggtgttgctc tgcgtcgtct
      601 gttcgacacc gatcgacagc ttgtcattca ggcgcttggc gatgccgaca tcggtgcccc
      661 cgctctcgtt ttccgtgatg gaaagctgat ccaggccgag ggcattctgc aggccgctca
      721 gcaacccgga atcaccacca cctgacagtg tagcgacata gctggcgatc tgcgcgatct
      781 gcagcggcga caactccagc atcgagttgt cgaagatcag ccgggccatc gcctcttcct
      841 gcggcatctg cggaaccgag gtgacctgga tggacggttc gctggcatag cccgaaaccg
      901 tcagcgtgat ggtggcatcc ttgcgccgcg acacggcgac gaagtcgatc ttcggatcaa
      961 gatcgcggtc gaaggtgagg ctgccgcgct cgaagtcgag gctgcgggcc ggaagggtca
     1021 gacggccccg caccatctgg aacgaaccat cggcggcaag attgtcgagc ggaccataga
     1081 tgcgaaggcc cccgttcagg atgacgttca gcccgcggcc gctgaccgag acttcatcca
     1141 gggcgcgcag ccggacgtcc acccggagac ttgagctttc cgtcggccgg ttgcggcgga
     1201 tctcacgcgc ctgcagggcc accgcgcgcg aggcattgat gtgcttgacg tcatcgggcg
     1261 tcagggcgcg tttcggtaat tcgctcaagg tgatcttggt gccgcgcagg tcgatttcgc
     1321 cggtcgccag gggcgaccgc aggagcggac cggtgatctt gagccgtgcg tcgtaacgcg
     1381 cggtgacgaa acttccatcg gtgtacatgc cattctcgac ccgcgcgtcg aggcttgcag
     1441 tgagaccggc gtccagcgcg atggtgccaa tcaccgtggc acggccattg cggcccgtga
     1501 caccactgag cgatgtgatc ctgacgaccc ggttgctgac atccgcactt ccggcggcgt
     1561 cgcgcagggt gaagcggccg gccgcgtcgc ccagaacgcc tttgcggagg gtgatctggc
     1621 cattgatgtc cggacggtcc agagcacccg acagtcgaag atcgaagccg acatcgccct
     1681 gggcacgcac accgctgcgc gccaagcggt cggcgaagag ggcgggaccc gtccggccct
     1741 tgagctccat gtcgatgcgc gatgttcgaa ggtcgaccgt gccgttgccc gtcaccgaaa
     1801 gctgcttgcc ggcatcggcc ttgagtgtgg ccctgagcgt cccaccgctg atcgtgccat
     1861 tggcgctggc cgacatcggc ctgacgccga gttgtttcag cgtctccgtc gaaagccctg
     1921 cgcccgacag cttgaagctg ccttcggggg ccgagaggct tcccgtcagc acggcctcgc
     1981 cggagagcgt gccctggagg cctggctgtc cgccaaggcc tgcaaaggcc gcaagcggca
     2041 gcgactggac agtcacggtc gccttgaggt caggcaacag acgtccaccg acccggatgc
     2101 tgcccttgcc gatggcaagg cgcgcgggtt caagcgtcac cgtctcgccc gcctgctgaa
     2161 ggacgagcgg tgcgctggcg gagaacggaa tgccgcgcag cttgcccgcg aggcgcgaca
     2221 ccgcatagcg ggcttcgccg ctgcccagcg tgacaacggc atcggcgtcg agggacagat
     2281 caggtcccgc cagcttcacc gcggtccgat aggtgttggt acctgtggcc tttgccgtga
     2341 cagtggcgga gtcgaagcgc tggccgccga tccgtgcctt gccaagcttc acctggccct
     2401 cgggccgcgg cacgccgaac agtgtggcga tgctgatgtt gccttcggcg gtggccgcgg
     2461 tgaaggacgg cgtcacgatg ttgcgcccat tgaagcggat ctcggtcgca tgggcttcgc
     2521 cagcgccgcg catctcgatg tcgccggttg ccgcgcccgc gagttcggtc aggaacagcg
     2581 gcgccacctc tgccagatcg ggtgccgaga agccgagggt gccggtgggc gcatcgccct
     2641 tcggccagcg cagttttccg gagagccttg cgcttccggc gatggccgtg agattctcca
     2701 agaccacgtc gcccgcggca tcggatgcga agctggccgc gatgtcgaca ggctttccgt
     2761 cgagacggcc actggcggtt aacgtgccgc tgggtgcggc attctcgatt ttcacttcag
     2821 ctgccgcgac aggcctttcg aggctcttgc ccatgagcgt gaccgcttcg ccccggccgt
     2881 tgatgcgcgc cgtcaggccc tgccatggac ccttgaggcc agcatcgagt tgcgcggtgc
     2941 cgcccagtcc ctcatggaca agggcaaggt cgggaagccg cacggccagc gccaggtctg
     3001 ccgccgcggc cgaagcgtgg ccggaagccg atgccgtcag tgccgcactg gtcagcgtga
     3061 gcccgtcgag gccgatgcca tcgccgttgc cgcccgtgat ccggccttcg gccgtcaccg
     3121 gtccggcaag cagcctgtcc actgcggaaa tgccggtctg gggactgtcg gccttgccct
     3181 gaatccgcag gtcgaaactg ccggttgccg tctcgatcgt cccgctgcct ccgagcgcaa
     3241 ccg
//
LOCUS       DBNGVR010000029         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4528717, whole genome shotgun sequence.
ACCESSION   DBNGVR010000029 DBNGVR010000000
VERSION     DBNGVR010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4528717"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="AC16A0_00620"
     CDS             <1..538
                     /locus_tag="AC16A0_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR3103857.1"
                     /translation="ISALVDKVAAGDALAVVAAEIASRNLPAVGGREVDEIAARLADK
                     AKDRRESPLAGEKAEQINRYLAISGEASGIDSQLKLIRGGDRFAGARDDYVRRLFEME
                     ELGLNPRRFHFAAGFGRELEYYTGFTFQVEADTPDGAVAVAGGGRYDNLLSDMGSPVR
                     VPAVGCAIHTDRLKAVLA"
     gene            535..1242
                     /gene="hisG"
                     /locus_tag="AC16A0_00625"
     CDS             535..1242
                     /gene="hisG"
                     /locus_tag="AC16A0_00625"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR3103858.1"
                     /translation="MSAVTLAIPSKGRLMESAALLLEAAGFGIERLGADRGYRGRLTG
                     IDGVDVAFLSASEIAQKLRDGAIDLGVTGEDLLRETIAPGDPAADVLLRLGFGPADVV
                     VAVPEIWLDVATMTDLDEIAESFYERYGRRLRVATKYLNLTRRFFAEKGVTGYRIVES
                     LGATEGAPAAGIAEIIVDITTTGSTLAANALRVLDDGVILRSCAVLAARAGVARAPAV
                     AALTAALESALQKGRTG"
     gene            1277..1567
                     /locus_tag="AC16A0_00630"
     CDS             1277..1567
                     /locus_tag="AC16A0_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103859.1"
                     /translation="MIKSVASLAVLALAFALPAHAQGNATPLGLNWSSGWSCCGGPVL
                     DQTIVIQVGNDNKARVVDTDEVDSGPRAVPLGLTNGSAGCNLYRGVYLCAYR"
     gene            1604..1834
                     /locus_tag="AC16A0_00635"
     CDS             1604..1834
                     /locus_tag="AC16A0_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103860.1"
                     /translation="MLKLFAAAAIAALSAAPALANDAVPPPAEAEVMVLPQSPVPAEE
                     AALAAPAEAASQVSPMGGHGCSSKRQQVYLTN"
     gene            complement(1845..>2733)
                     /locus_tag="AC16A0_00640"
     CDS             complement(1845..>2733)
                     /locus_tag="AC16A0_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3103861.1"
                     /translation="YVAMGIEADTEITVPDEQKAEVALVLDYSGSMTETSGGQVKYVA
                     MKNAAKKLITDLETTNEGKVKFGLVPFSHHVYVTLPKAYVTGTTGSGDWTGCTQDRRH
                     PHNLTDATPTGSNATKWGHPHAPVHSSDGCWAYAPKSLKVAPLTEDFDALRTQLDNMQ
                     PYAWTHIALGAEFGYHLLSPNEPFTEGAAYSDDDTSKFLVILTDGRQTEPAFGPSSRT
                     VSQGEDNLEAICSNAKASGITVMTIAFDLQDEDTTNRLRDCASDPAQNFMIAEDSSDI
                     AAAFADIREQIAAQVFISR"
BASE COUNT          437 a          855 c          916 g          525 t
ORIGIN      
        1 catctcggca ttggtggaca aggttgctgc tggtgacgcc ctcgccgtgg ttgcggccga
       61 gatcgctagc cgtaatcttc cggccgtggg cgggcgggaa gtggatgaga tcgcagcgcg
      121 attggcggac aaggccaagg atcgtcgtga gtcgccattg gcaggcgaga aggcagagca
      181 gatcaatcgc tacctcgcga tttcgggtga agcttccggc atcgactcgc aactgaaact
      241 gatccggggt ggcgatcggt tcgccggcgc gcgcgacgac tatgtccgcc ggctcttcga
      301 aatggaggag cttggcctca acccgcgtcg cttccatttt gctgcaggct ttggccggga
      361 gctggaatac tacaccggct tcacctttca ggtggaggcc gacaccccgg atggcgccgt
      421 cgccgtggcg ggcggcgggc gctacgacaa tcttctctcc gacatgggat cgccagtgcg
      481 cgtgccggcg gtcggctgcg ccattcacac cgaccggctg aaggcggtgc tggcatgagc
      541 gccgtcaccc tggccatacc gtcgaagggc aggctgatgg agagcgcggc cctgctgctg
      601 gaagcggcag gcttcggaat cgagcgcctg ggcgccgacc ggggctatcg cggccgactg
      661 accggcatcg acggcgtgga tgtggcattc ctctcggcct ccgaaatcgc ccagaagctt
      721 agggacgggg cgatcgatct cggcgtgacg ggcgaggacc ttctccgcga gacgatcgcc
      781 ccgggtgacc cggcggccga cgtgctgctg cgcctgggct ttggcccggc cgacgtggtg
      841 gtcgccgtgc ccgagatctg gctcgacgtc gcgacgatga ccgatctgga cgagattgcc
      901 gagagcttct acgagcgcta tggccgcagg cttcgagtcg cgacgaaata tctgaacctg
      961 acaaggcgct tctttgccga gaagggcgtc accggctacc ggatcgtcga gagccttggt
     1021 gcgaccgagg gtgcaccggc tgcgggcatc gccgagatca tcgtcgacat caccacaacc
     1081 ggctccaccc tcgccgccaa tgccctgcgg gtgctggatg acggggtcat cctgaggtcc
     1141 tgtgccgtgc tcgccgcccg cgccggcgtg gcccgggcgc ccgccgtggc cgcactgacc
     1201 gccgccctag aatccgcttt gcaaaagggc cggactggtt aatacttcgt tgctggtctg
     1261 tatcggagta ggtcacatga tcaagtcagt tgcgtctctt gccgtgcttg ccctcgcctt
     1321 cgccctgccg gcccatgccc agggcaatgc cacccccctc ggactgaact ggagcagcgg
     1381 ctggtcctgt tgcggcgggc ccgtgctcga tcagacaatc gtgatccagg tgggtaatga
     1441 caacaaggcc cgtgtcgttg atactgacga ggttgactcg ggcccgcgtg cggtgccgct
     1501 cggactgacg aacggttcgg ccggctgcaa tctctatcgc ggcgtctatc tctgcgccta
     1561 ccgctgacgc cggagcgtcg gcggaacaca agggagtttt gacatgttga agctctttgc
     1621 cgccgccgcc attgcagccc tttccgccgc gccggcgctg gccaacgatg ccgttccgcc
     1681 acccgccgaa gccgaggtga tggtcctgcc gcagtcgccg gttccggccg aggaggccgc
     1741 acttgccgcg cccgccgaag ccgccagcca ggtgtccccc atgggtggcc acggttgcag
     1801 cagcaagcgc cagcaggtct atctgaccaa ctgaggcctg ggcctcagcg gctgatgaag
     1861 acctgcgcgg cgatctgttc gcggatgtcc gcgaaggcgg ctgcgatgtc gctgctgtct
     1921 tcggcaatca tgaagttctg agccggatcg ctggcgcagt cgcgcagccg gttggtcgtg
     1981 tcttcgtcct gcaggtcgaa ggcgatggtc atgaccgtga tgcccgaggc cttggcattg
     2041 ctgcagatgg cctcaagatt gtcctcgccc tgcgagaccg tgcgcgaact gggaccgaag
     2101 gccggttccg tctgccggcc atcggtgagg atgaccagga acttgctggt atcgtcatcg
     2161 ctgtaggcag cgccttcggt aaagggttcg ttgggcgaca gcaggtgata accgaactcg
     2221 gcgcctagcg cgatatgcgt ccaggcatag ggctgcatgt tgtcgagctg cgtgcgcagc
     2281 gcatcgaagt cttccgtcag cggcgcaacc ttcagggact tcggcgcata ggcccagcag
     2341 ccgtccgatg aatggacagg ggcatgcgga tgaccccact tggttgcatt gctgccggtc
     2401 ggcgtcgcgt cggtcagatt atgcggatgg cgcctgtcct gggtgcagcc ggtccagtcg
     2461 ccgcttccgg tcgtgccggt gacataggcc ttgggcagtg tcacgtagac atgatgcgag
     2521 aagggcacga ggccgaactt caccttgcct tcattggtgg tctcgagatc ggtgatgagc
     2581 ttcttggcgg cgttcttcat cgccacatac ttgacctggc cgcccgaggt ctcggtcatc
     2641 gagcccgagt aatcaagaac aagggccact tcggccttct gttcatcagg caccgtgatc
     2701 tcggtgtccg cctcgatgcc catggccaca tat
//
LOCUS       DBNGVR010000030         1756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1779612, whole genome shotgun sequence.
ACCESSION   DBNGVR010000030 DBNGVR010000000
VERSION     DBNGVR010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1779612"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="AC16A0_00645"
     CDS             complement(<1..300)
                     /locus_tag="AC16A0_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3103862.1"
                     /translation="MEVFLQQCVNAISLGGIYALLALGLAVVFSIVRLINFAHGEVMT
                     IAGYAIWLALLSSVPVVAAIILGITVAMLASVAMERIAFRAMRGASVTSLLITSFA"
     gene            complement(305..1009)
                     /locus_tag="AC16A0_00650"
     CDS             complement(305..1009)
                     /locus_tag="AC16A0_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3103863.1"
                     /translation="MLSVEGLVVRYGPIEAVRGIDFTVNRGEVFALLGANGAGKSSTL
                     AAIAGLVKPFAGRIAYDGKPVSGLPPETLSRAGIGLVPEGRRIFASLTVAENLWLGGA
                     AHGSTAERQQLQKEMEERFPILGERARQKAGLLSGGEQQMLAIARALMARPKLLLLDE
                     PSLGLAPQMVEKVFDLIAAMRKEGLTILLVEQNVPMSLQIADRGIVLANGHIVVSGTG
                     ADLLHNSDVTNAYLAA"
     gene            complement(1019..>1756)
                     /locus_tag="AC16A0_00655"
     CDS             complement(1019..>1756)
                     /locus_tag="AC16A0_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3103864.1"
                     /translation="GSYPGDNQQAAVLGAYAAELGYKTAWLLKSPDSSYTMKGPEYFG
                     QVFEAKGGKVIGEGSYTLNQPDFSAIVTTIKALDPQPDVIATWAWEPDFPAFIKALRG
                     AGVTTPVMGADVLDTPTVRGLGEVVNGIVHTSGGYPEPGSKHADFNARFKAATGMEAD
                     TNYVVNGCDIALMIDAAVTAAGSSDPKAVRDALANLKDSPGNMSNFTFAGTDRMPLRE
                     VVLAKIENGQKTFIRRQLADPATMPKP"
BASE COUNT          315 a          558 c          580 g          303 t
ORIGIN      
        1 cgcaaaggag gtgatcagca acgaggtgac gctcgcaccg cgcatggcgc ggaatgcgat
       61 gcgttccatg gcgacggagg ccagcatcgc gacagtgatg ccgagaatga tggccgcgac
      121 gacaggaacc gacgagagca gggcaagcca gatcgcatag cccgcgattg tcatgacctc
      181 gccgtgtgcg aagtttatca gccgcacgat ggagaagacc accgcgagac caagggccag
      241 cagcgcataa atgccgccga ggctgatcgc attgacgcac tgctgaagga agacttccat
      301 gtgctcacgc tgccagatag gcgttggtga cgtcgctgtt gtgcagcaag tcggcacctg
      361 tgccggaaac aacgatgtgg ccgttggcga gaacgatgcc gcggtcggcg atctggagcg
      421 acatcggaac gttctgctcg accagcagta tagtgaggcc ctccttgcgc attgcggcga
      481 tcaggtcgaa gaccttctcc accatttggg gcgcaagacc gagcgagggc tcatcgagca
      541 gcagcagctt cggccttgcc atgagggcgc gggcgatggc gagcatctgc tgttcgcctc
      601 ccgacaggag tccggccttc tgccgcgcgc gctcccccag gatcggaaaa cgctcctcca
      661 tctccttttg gagctgctgg cgttccgccg tgctgccatg agcggcgccg ccgagccaca
      721 ggttctccgc caccgtgagg ctcgcgaaaa tgcgccggcc ttcgggcacc agcccgatgc
      781 cggcgcgcga cagggtctcg ggcggcaagc cggagaccgg cttgccgtca taggcgatgc
      841 ggccggcgaa gggtttcacc aggcccgcga tggcggcgag cgtagagctc ttgccggcac
      901 cattggcgcc aagcaatgcg aagacctcac cgcgattgac ggtgaagtcg atgccgcgaa
      961 cggcctcgat cggcccgtag cgcacgacaa gtccctctac gctcagcatg cgtctggctc
     1021 agggcttcgg catggtcgcc ggatcggcga gctgccgccg gatgaaggtc ttctgaccat
     1081 tctcgatctt cgccagaacc acctcgcgca acggcatgcg gtcggtgccg gcgaaggtga
     1141 agttgctcat gttgccgggc gaatccttga ggtttgccag cgcatcgcgc accgccttgg
     1201 gatcgctgga gccggcagcc gtaacagcag cgtcgatcat caatgcgatg tcgcagccgt
     1261 tcaccacgta gttcgtgtca gcctccatgc cggtggcggc cttgaaccgc gcgttgaagt
     1321 ccgcatgctt cgagcccggc tcgggatagc cgcccgaggt gtgtacgatg ccgttgacca
     1381 cctcgcccag accgcgcaca gtcggcgtgt cgagcacgtc ggcacccatc accggtgtcg
     1441 tcacgcctgc gccgcgtagc gccttgatga aggccgggaa gtccggctcc caggcccagg
     1501 ttgcgatgac gtcgggctgc gggtcgagcg ccttgatggt ggtcacgatg gccgagaagt
     1561 caggctggtt cagcgtgtag gagccctcgc cgatcacctt gccgcccttc gcttcgaaca
     1621 cctggccgaa atactccggg cccttcatgg tgtaggacga gtcgggcgac ttcaggagcc
     1681 aggcggtctt gtagcccagc tctgcggcat aggcgccaag cacggcggcc tgctggttgt
     1741 ctcccggata gctgcc
//
LOCUS       DBNGVR010000031         4023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2369194, whole genome shotgun sequence.
ACCESSION   DBNGVR010000031 DBNGVR010000000
VERSION     DBNGVR010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2369194"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..2301
                     /locus_tag="AC16A0_00660"
     CDS             73..2301
                     /locus_tag="AC16A0_00660"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR3103865.1"
                     /translation="MDQKPAIPQDAIAQEIWDMKYRLKSADGTPIERTVDETWWRVAR
                     AIAMAEAPAQREDWARAFHDAMSGFRFLPAGRILAGAGTGRSVTLFNCFVMGTLPDSL
                     AGIFDGLKEAALTMQQGGGIGHDFSTLRPKGAPVRGVGADASGPLSFMDVWDSMCRTI
                     MSAGARRGAMMGTLRCDHPDIEAFVAAKREARRLRNFNLSVLVTDAFMQAVKDDGDWP
                     LVFGGVTFRVIRARDLWEQIMRATYDYAEPGVIFIDRVNAANNLAYCETIGCTNPCGE
                     QPLPPYGACLLGSINLARLVADPFTAKARLDDSALRAITAVAVRFMDNVIDVSNYPLE
                     AQRAEAMAKRRIGLGLTGLADALAMCGLVYGTDEAAAAAAQWMATIEHAAYRASADLA
                     RERGAFPLFDPARFGMSGHAASLPKELRALISAHGIRNGLLTSIAPTGTISLFAGNVS
                     SGIEPIFSLGYDRRILQPDGSSRSERVTDYALGVWRELHGDTPPPEAFVTAETLEPAA
                     HLRMQAAVQRHVDSSISKTINVPADLAFDDFKAVYEQAYDMGLKGCTTFRPNDITGSV
                     LSTAQSVAQAPLDRAEVLPGFTYKLRWPESDHAIYITMNDIVLDGVRRPFEIFINSKN
                     MEHYAWTVALTRMISAVFRRGGDVGFVVEELKAVFDPRGGQWMEGRYVPSLLAAIGGI
                     IERHMLEIGFLKAPVSLNAAAATGDDGGPKRSACPRCGERALVFQEGCATCLSCGFSK
                     CS"
     gene            2346..2705
                     /locus_tag="AC16A0_00665"
     CDS             2346..2705
                     /locus_tag="AC16A0_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103866.1"
                     /translation="MLNGFPISAKLPDDLGIGERFWYWRGASGQTYIHSIYPAGSCPP
                     LPGAVFLAVRRHGDGRREVLRAGRFPDDWDGPNGAQNLMADEIHVHLLARGDAAAVAV
                     LGDLAAAMKTASEKIAA"
     gene            complement(2702..3223)
                     /locus_tag="AC16A0_00670"
     CDS             complement(2702..3223)
                     /locus_tag="AC16A0_00670"
                     /EC_number="4.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR3103867.1"
                     /translation="MPEFWVFGYGSLMWKPGFAFLDSEPALLRGLHRRLCVLSHVHRG
                     TPERPGLVLGLDRGGSCHGMAFRIGPEHRAATLDYLRAREQVTMVYLEQVKPITLIGS
                     GRRVEAVTYVVDRSHRQYAGRLDDAQLAELVRQGEGQSGACHDYVRNTVAHLRDMQIH
                     DAGLERLMRRLEP"
     gene            3256..>4023
                     /locus_tag="AC16A0_00675"
     CDS             3256..>4023
                     /locus_tag="AC16A0_00675"
                     /inference="COORDINATES: protein motif:HMM:NF021423.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HIR3103868.1"
                     /translation="MKEQKPAPRPWRMLFPLFAVLALGLAWSAYWIIASSLAQKAVAE
                     RRAALAERGQRLDCSEERWGGFPFRFEFTCADPVLTADAAGTAQAPRLRAVAMAYNPW
                     HLLLLVDGPTRLSRRDGAELTLNHDGILASLRFRDQTTPPDVSVEIPKLDIVGWYTAD
                     DILIHTRPEADGAMGIALTASMSNYKPEGRPPLTVAAGSLRATLRPDGVLAVANIELQ
                     EGQTIYKGQGEVRLDAVHRIAGQLATETNDFDGLMRIL"
BASE COUNT          656 a         1363 c         1309 g          695 t
ORIGIN      
        1 gaagggcggg ccatgtacaa acagtgaacg aattctcgac tcgttagggt cttgggcgta
       61 agctcgcacg ccatggacca gaagcccgcc attccgcagg atgccattgc ccaggaaatc
      121 tgggacatga agtatcggct caaatccgcc gacggcaccc ccatcgaaag gacggtggac
      181 gagacctggt ggcgcgtggc ccgggccatc gccatggccg aggcccccgc acagcgcgaa
      241 gactgggcca gggcctttca cgatgccatg tccgggttcc gcttcctgcc ggcgggccgt
      301 attctggccg gcgcgggaac cgggcgctcg gtcacgctct tcaactgctt cgtgatggga
      361 acgctgccgg attcgctggc cgggatcttc gacggcctga aggaagcagc cctcaccatg
      421 cagcagggcg gcggcatcgg ccatgacttc tcgacgctgc ggcccaaggg cgcgccggtg
      481 cgcggcgtgg gggccgatgc ctcgggtccg ctgagcttca tggatgtctg ggactccatg
      541 tgccgcacca tcatgtcggc gggcgccagg cgcggcgcga tgatgggcac gctgcgctgc
      601 gaccatcccg acatcgaagc ctttgttgct gccaagcgcg aggcgcgccg cctgcgcaat
      661 ttcaatctct cggtcctggt caccgatgcc ttcatgcagg cggtgaagga cgacggcgac
      721 tggccgctgg tcttcggcgg cgtcaccttc cgcgtgatcc gggcgcgcga tctgtgggaa
      781 cagatcatgc gggccaccta tgactatgcc gagcctggcg tcatcttcat cgaccgtgtc
      841 aatgccgcga acaatctcgc ctattgcgag accatcggct gcaccaatcc ctgcggcgag
      901 cagccgctgc cgccctatgg cgcctgcctg cttggttcca tcaatctcgc gcggctggtg
      961 gcggatccat tcaccgccaa ggcgcgtctc gatgactccg cccttcgcgc catcaccgcg
     1021 gtggccgtgc gcttcatgga caatgtcatc gacgtgtcga actacccgct tgaggcccag
     1081 cgcgccgagg ccatggccaa gcgccgcatc ggcctggggc tcaccggtct cgccgatgca
     1141 ctggccatgt gcggcctggt ctatggcacg gatgaggcgg ccgcagccgc ggcgcagtgg
     1201 atggcgacga tcgagcacgc cgcctatcgc gccagtgccg atcttgcgcg ggagcgcggc
     1261 gcctttccgc tcttcgatcc cgcgcgcttc ggcatgtccg gtcacgcggc cagcctgccg
     1321 aaggagctgc gcgcgctgat ctccgcacat ggcatccgca acggcctgct cacctcgatc
     1381 gcaccgaccg gcaccatctc gcttttcgcc ggcaacgtgt cgagcggcat cgaaccgatc
     1441 ttcagcctgg gatacgaccg ccgcatcctg caacccgacg gcagcagccg cagcgagcgc
     1501 gtgaccgact acgcgctggg cgtctggcgc gaactgcatg gcgacacgcc gccgcccgag
     1561 gccttcgtca ccgccgagac gctggagccg gccgcccatc tccgcatgca ggcggcagtg
     1621 cagcgccatg tcgacagctc catctcgaag accatcaatg tcccggccga tcttgccttc
     1681 gacgacttca aggcggtcta cgagcaggcc tatgacatgg gcctcaaggg ctgcacgact
     1741 ttccggccca acgacatcac gggatcggtg ctctcgaccg cgcagtccgt cgcacaggcg
     1801 ccgctcgatc gtgccgaggt tctgcccggc ttcacctaca agctgcgctg gcccgagagc
     1861 gaccatgcca tctacatcac catgaacgac atcgtgctcg acggcgtgcg gcgccccttc
     1921 gagatcttca tcaactcgaa gaacatggaa cactatgcct ggacggtggc gctcacccgc
     1981 atgatctccg ccgtgttccg ccgcggcggc gacgtgggct tcgtggtgga agagctgaag
     2041 gccgtcttcg atccgcgcgg cgggcagtgg atggaagggc gctatgtgcc aagcctcctg
     2101 gcggccatcg gcggcatcat cgagcggcac atgctggaga tcgggtttct caaggcgcct
     2161 gtgtccctga acgcggcggc ggcaacgggc gatgacggag ggccgaagcg cagcgcctgc
     2221 ccccgctgcg gcgagcgcgc gctggtgttc caggagggct gcgccacctg cctgtcctgc
     2281 gggttctcca aatgtagctg agggagggct tgacagtttc ggcattttgt tctagttttg
     2341 ttctcatgct gaacggcttc cccatctctg caaaactgcc tgacgatctc gggatcggcg
     2401 agcgcttctg gtactggcgc ggcgcttcgg gccagaccta cattcactcc atctatccgg
     2461 cggggtcctg cccgccgctg ccgggcgcgg tgtttctggc cgtgcgccga catggtgacg
     2521 gcaggcggga ggtcttgcgc gccgggcggt ttccggatga ctgggacggc cccaatggcg
     2581 cccagaacct gatggcggac gaaatccacg tgcatctcct ggcacggggc gatgctgccg
     2641 cagtggcggt gctcggcgat ctggccgccg cgatgaaaac ggcctccgag aagattgccg
     2701 cctaaggctc cagccgtcgc atcagccgtt cgagaccggc gtcgtggatc tgcatgtcgc
     2761 gcagatgcgc cacggtattg cgcacatagt catgacaggc gccggactgg ccttcgccct
     2821 ggcgcacgag ttcggccagc tgcgcgtcat ccagccggcc ggcatattgc ctgtggctgc
     2881 ggtcgaccac ataggtgacc gcttccaccc tgcggccact gccgatgagg gtgatgggct
     2941 tgacctgttc gagatagacc atggtcacct gttcgcgggc gcgcagatag tcgagcgtcg
     3001 ccgcgcgatg ctccgggccg atgcggaaag ccatgccatg acaggagccg ccacggtcga
     3061 ggcccagtac caggcccggc cgttcgggcg tgccgcgatg cacatgcgag agcacgcaaa
     3121 gccggcggtg cagcccgcgc agcagcgcgg gctcgctgtc aagaaaggca aagcccggct
     3181 tccacatcag cgagccgtag ccaaagaccc agaattccgg catggtttgc gctgtttaca
     3241 aagatgtcgg tcgcgatgaa agagcaaaag ccggcgccac gtccctggcg catgctgttc
     3301 cccctgtttg ccgttctggc cttgggcctt gcctggagtg cctattggat catcgcctcc
     3361 agcctggccc agaaggcggt ggccgagcgc cgcgcggcgc ttgctgaacg cggccagcgc
     3421 ctcgactgca gcgaagagcg ctggggcggc tttcccttcc gtttcgaatt cacctgtgcc
     3481 gacccggtgc tgacggctga cgcggccgga acggcgcagg cgccgcgcct tcgtgccgtg
     3541 gccatggcct ataatccctg gcacctgctg ctgctggtcg acgggccgac ccggctgagc
     3601 cggcgcgacg gggccgagct tacgctcaac catgacggca tcctcgccag cctgcgcttc
     3661 cgcgatcaga ccacgccgcc cgacgtgtcg gtcgagattc cgaaactcga tatcgtcggc
     3721 tggtacaccg ccgatgacat tctgattcac acgcggcccg aggccgatgg cgccatgggc
     3781 atcgcgctga ccgccagcat gtcgaactat aagccggaag ggcggccgcc gctcacggtg
     3841 gcggccggat cgctgcgcgc cacgctgcga ccggatggtg tgctggccgt cgccaacatc
     3901 gaacttcagg aaggccagac catctacaag ggccagggcg aggtgaggct cgacgccgtg
     3961 catcgcatcg ccggccagct tgccaccgag acaaacgact tcgacggcct catgcgcatt
     4021 ctc
//
LOCUS       DBNGVR010000032         2731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4922932, whole genome shotgun sequence.
ACCESSION   DBNGVR010000032 DBNGVR010000000
VERSION     DBNGVR010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4922932"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="AC16A0_00680"
     CDS             complement(<1..876)
                     /locus_tag="AC16A0_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3103869.1"
                     /translation="MTAAVHPDRNFKYWLVAPAIFLLLLIGLFPLIYSLVVSFMRIDM
                     MDTDTSFAGLYNYANLFKDSRLWWSLAHTLLITAIALPLELLLGLAMAYLFLEKMPGR
                     QIFISLLVLPTIISPIVAGATWRLLFDNKYGPINQILSFFAGERVNALWTINPTLVYP
                     AIIFCEIWQWTPFMFLILLAALSNVDKSQTEAAEIDGASFIRTFRYVVLPAIWPVMAI
                     AILIRGLDLFRIFDIIWALTQGGPGTKTETISIYAYSQGFKQFETSYTAAMAFLVVFL
                     LTVIVTWAIRRMGLAK"
     gene            complement(964..2415)
                     /locus_tag="AC16A0_00685"
     CDS             complement(964..2415)
                     /locus_tag="AC16A0_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3103870.1"
                     /translation="MVTRRDVGKIGIAAGLAGTLPTRAALAADGAATAVEAAKQYSGQ
                     TITIVWEAGLQSLDPLNFSGPKWKELTGIDVKVVEVPVAEMFTKIMQDFRSGAGAYDA
                     LNVIPSWMPDLANAGALEVLDSFVDKYGYRDELMKIASTYRDNQMMVNGKIYGLPDDG
                     DVFVLYYRKDVLTDPAIQKAYKDKYGADLPVPPTTWKDFDQVASLITEVTGGKPYGAA
                     FFREPGYGNFMFQERFRNEGGKFFDAETMKATINTDAGVKVFTDWLAENKYAPAGVEQ
                     WGFVENLAAFLQGQTAMTISWPPYGRWAASYGTDQEVMSWVPKSTIAGKVGYAMTPGG
                     HPELAAGFCLSVSSASKNKDAAYLFIQWLNSEAVSLERVKLPYALRDPFRDSHFTDPG
                     YAALWPDAKDYLKALQDGANNGLLDLSVLQTDKYEEVLRQSVSKLWSGEDPKAILDAA
                     AAEWDAITEKIGVDKQKAVYADWASKSGAYPKM"
     gene            complement(2491..>2731)
                     /locus_tag="AC16A0_00690"
     CDS             complement(2491..>2731)
                     /locus_tag="AC16A0_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HIR3103871.1"
                     /translation="VFEQEPTDPDDPLFKLDNIIVAPHALCWTDQCFAGNGAADVKAV
                     LEVQKGQVPRGVVNRDVLERDGFKKKLEGFAKRFG"
BASE COUNT          515 a          820 c          880 g          516 t
ORIGIN      
        1 tttcgccagc cccatgcggc gtatcgccca ggtgacgatc acggtgagca ggaacacgac
       61 gagaaaggcc atcgccgccg tgtagctggt ttcgaactgc ttgaaccctt ggctataggc
      121 gtagatcgat atggtctcgg tcttggtgcc cggaccaccc tgggtcagcg cccagatgat
      181 gtcgaagatg cggaaaaggt cgaggccgcg gatcaggatg gcaatggcca tcaccggcca
      241 gatggcgggc agcaccacat accggaaggt gcggatgaag gacgccccgt cgatctcggc
      301 ggcctccgtc tgcgacttgt cgacgttgga gagggccgcc agcaggatca ggaacatgaa
      361 gggcgtccac tgccagatct cgcagaagat gatggcggga tagaccagcg tcgggttgat
      421 ggtccacagg gcgttgacgc gttcgcccgc gaaaaagctg aggatctggt tgatcggccc
      481 atacttgttg tcgaacagca ggcgccaggt ggcgccggcg acgatgggtg aaatgatggt
      541 cggcagcacc agcaatgaaa tgaagatctg gcggcccggc atcttctcga gaaacaggta
      601 ggccatggca aggccgagca gcaattcaag cggcagcgcg atggcggtga tcagcagcgt
      661 gtgcgccagc gaccaccaga gccgggaatc cttgaacagg tttgcgtagt tgtagagccc
      721 cgcgaaggac gtgtccgtgt ccatcatgtc gatgcgcatg aagctcacga caagcgaata
      781 gatcagcgga aagaggccga tgagcaaaag gaggaagatg gccggcgcca ccagccaata
      841 cttgaaattg cgatcgggat gaacggccgc cgtcatgcgt gcagattgct tctgaaaatc
      901 ttcgggatca atgcccttgg acgaatggcc tccccccgca aaggagggag gccaccggtg
      961 acgttacatc ttgggatagg cgccggactt ggacgcccag tcggcataga cggccttctg
     1021 cttgtccacg ccgatcttct cggtgatggc atcccactcg gccgcggcag cgtcgaggat
     1081 ggccttcggg tcctcgcccg accacagctt ggacaccgac tggcgcagca cttcctcgta
     1141 cttgtcggtc tgcagcaccg acaggtcgag aaggccgttg ttggcgccgt cctgcagcgc
     1201 cttcaggtag tccttggcgt cgggccacag cgcggcataa cccggatccg tgaagtgcga
     1261 gtcgcggaag gggtcgcgca gcgcataggg cagcttcacg cgttcgaggc tgacggcctc
     1321 gctgttcagc cactggatga acagataggc ggcatccttg ttcttcgacg ccgacgagac
     1381 cgagaggcag aagccggcgg ccagttccgg atgtccgccc ggcgtcatgg cataaccgac
     1441 cttgcccgcg atggtggact tgggaaccca gctcatgact tcctggtccg tgccatagct
     1501 cgcggcccag cggccatagg gcggccagga gatggtcatg gccgtctggc cctgcaggaa
     1561 ggcggcaagg ttctcaacga agccccactg ctccacgccg gccggggcat acttgttctc
     1621 ggccagccag tcggtgaaga ccttgacgcc tgcatcggtg ttgatcgtgg ccttcatggt
     1681 ctcggcgtcg aagaactttc cgccctcgtt gcggaagcgc tcctggaaca tgaaattgcc
     1741 gtagcccggt tcgcggaaga aggccgcgcc atagggcttg ccgccagtca cttcggtgat
     1801 gaggctggca acctggtcga agtccttcca ggtcgtcggc ggcaccggca gatcggcgcc
     1861 gtacttgtcc ttgtaggcct tctggatcgc cggatcggtg agcacgtcct tgcggtagta
     1921 caggacgaag acgtcgccgt cgtccggcag gccgtagatc ttgccgttca ccatcatctg
     1981 gttgtcgcgg taggtcgatg cgatcttcat cagctcgtcg cgatagccat atttgtcgac
     2041 gaaggaatcg agcacctcga gggcgccggc attggcgagg tcgggcatcc aggacgggat
     2101 gacgttgagc gcgtcatagg cgcccgcgcc cgagcggaag tcctgcatga tcttggtgaa
     2161 catctcggcc accggaacct cgacgacctt gacgtcgatg ccggtgagct ccttccattt
     2221 cggaccggag aagttcagcg ggtccagcga ctgcaggccc gcctcccaga cgatggtgat
     2281 cgtctggccg ctatactgct tggcggcttc caccgccgtt gccgcgccat cggcggcgag
     2341 tgccgcgcgt gtcggcaggg tacccgcaag cccggcggca atgccgatct tgcctacgtc
     2401 acgtcttgtt accattgtcg tcctcccgtt tgagaatggc tgcgacaggc ggccattcga
     2461 gtctcccccg tgtcctccag gggaattgca tcacccgaag cgcttggcga agccttcgag
     2521 ctttttcttg aagccgtcac gttccagcac gtcgcgattg accacgccgc gcggaacctg
     2581 tcccttctgc acctcgagca ccgccttcac gtccgccgca ccgtttccgg cgaagcattg
     2641 gtcggtccag cacagcgcat gcggtgccac gatgatgttg tcgagcttga agagcggatc
     2701 gtcaggatcg gtcggctcct gttcgaagac g
//
LOCUS       DBNGVR010000033         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4530799, whole genome shotgun sequence.
ACCESSION   DBNGVR010000033 DBNGVR010000000
VERSION     DBNGVR010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4530799"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1043)
                     /locus_tag="AC16A0_00695"
     CDS             complement(84..1043)
                     /locus_tag="AC16A0_00695"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3103872.1"
                     /translation="MTRDYRKPLLKSPHLLAIDDVSAMEALALLDLAETYVEVSRQID
                     KRHAVLQGRTQVNLFFEASTRTQSSFEIAGKRLGADVMNMAVATSAVSKGETLLDTAM
                     TINAMHPDFLVVRHPSSGAVELLAQKVSCAVINAGDGQHEHPTQALLDALTIRRRMGR
                     LEGIVVAICGDVLHSRVARSNIHLLNKLGAQVRVVAPSTLLPSGIARFGVDIHRNMTD
                     GLAGADVVMMLRLQMERMSGAFVPSQREYYRFFGLSEDKLRYAKPGALVMHPGPMNRG
                     IEIDSNVADGVQSVIREQVEMGVAVRMAVLDALSRNMPSGGNA"
     gene            complement(1114..2457)
                     /locus_tag="AC16A0_00700"
     CDS             complement(1114..2457)
                     /locus_tag="AC16A0_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR3103873.1"
                     /translation="MDKILAWLESRTDIKSLRAAVCDLNGVMRGKRIPLEQAEKVLSG
                     GLRMPLSVVGVDIWGEDIVKSPLVFATGDADGTCESTGRGILPVDWTAEPSGLIPLWL
                     REESGVPFGGDPRRALAALVDRFKAHGLTPVMAMELEFYLYDPTNARPIAPVSPVTGK
                     RLDADGVLSIDELDDFGSFFEEVYAACAAQGVPADSAIAENGIGQFEINLMHVDDPLR
                     AADDAVLFKRIVRGLARKHGFAASFMAKPYGERSGSGLHVHFSLLDSKGRNVFDDGSN
                     DGSTTMRSAVGGLIESMPETTLLYAPHFNSYRRLRPGTHAPSAAAWGYENRTVAIRIP
                     GGSSKARRIEHRVAGADANPYLVLAGILAGALHGIEGEISPPDPVTGDAYELKLKSLP
                     PDWASAIDAFAAGSIVERTLPLMLRDMLVACKRQEMATFALQVTDFEYETYLDAV"
     gene            complement(2527..>3442)
                     /locus_tag="AC16A0_00705"
     CDS             complement(2527..>3442)
                     /locus_tag="AC16A0_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3103874.1"
                     /translation="LLPVFIVILIGWALRRFGVLVDDHWQALDRLCYFVLFPAIIFKE
                     IAGADFSSVPVLGMALSMVLALAVMFAALLILNRPLMRVLSIDGPQFTSLFQGATRWH
                     TFVAFAIIPLIFGADALSLGAVSAATMTPLLNIVCVVVMARFAHTHRPSAGTLALSII
                     RNPFVFSSLGGVAWQVLDLPVHEMGIQVLDMIGRGALGLALLTVGAGLRFGEAITSWL
                     PIAAAAILKLLVLPVVMAVMLHLFGVTGEAYAVALICAAVPTGSGAYVLARQMGGDAP
                     MVANILTVQVIFAAVTIPLVLTLLGAIP"
BASE COUNT          689 a         1067 c         1107 g          579 t
ORIGIN      
        1 gcccatcaag ccctgggccg gcgccacgat ctcggcattg agataggccc tgcgggttgc
       61 tgcggttctg gcggcgggcg ccatcatgcg ttgcctccgg aaggcatgtt gcgcgacagc
      121 gcgtcgagca cggccatgcg aacggcgaca cccatctcga cctgctcgcg gatgaccgat
      181 tgcacgccat ccgcgacgtt cgagtcgatc tcgatcccgc ggttcatcgg gccgggatgc
      241 atcaccagcg ccccgggctt ggcatatctg agcttgtcct cgctgagccc gaagaagcgg
      301 tagtattccc gctgcgaggg cacaaaggca ccggacatgc gttccatctg caggcgcagc
      361 atcatcacga cgtccgcgcc ggccaggcca tcggtcatgt ttcgatggat gtcgacacca
      421 aatcgcgcga tcccggacgg gagcagggtg gacggcgcca ccacccgaac ctgcgcgccc
      481 agcttgttca gaagatggat gttggaacgc gccacccgcg agtgcaggac atcgccgcag
      541 atcgccacca cgatgccctc cagtcggccc atccgccgtc ggatggtgag ggcgtccaaa
      601 agcgcctggg ttggatgttc atgctggcca tcaccggcat tgatgacggc acaggaaacc
      661 ttctgggcca gcagttccac ggcgccggat gatgggtgcc gcaccaccag gaaatcgggg
      721 tgcatggcat tgatggtcat ggccgtgtcg agcagggttt cccccttgga cacggcagac
      781 gtggcgaccg ccatgttcat gacgtcggcg cccagcctct tgccggcgat ctcgaaagaa
      841 ctctgggtcc gggttgacgc ttcgaagaag agattgacct gcgtccgtcc ctgcagaacg
      901 gcatggcgct tgtcgatctg acggctcacc tcgacatagg tttcggcgag gtcgagcagg
      961 gccagggctt ccattgcgga aacatcgtcg atcgccagta aatgcgggct cttgaggagc
     1021 ggcttgcggt aatcacgggt cattaaagcc gggtgtgtac gggtgaatcc ccgtctccgc
     1081 aagcctcaag ttgcggatgt ggaaacatgc ttatcagacg gcatccaggt aggtctcgta
     1141 ctcgaagtcc gtcacctgca gggcaaaggt cgccatctcc tggcgcttgc aggccaccag
     1201 catgtcgcgc agcatcagcg gcagcgtcct ttcaacgatg gacccggccg caaaagcgtc
     1261 gatggccgaa gcccagtcag gcggcagcga cttgagcttg agttcatagg catcgccggt
     1321 taccggatca ggcggcgaga tctcgccttc tatgccatga agcgcgccgg ccaagatccc
     1381 cgccaggacg agatagggat tggcatcggc cccggcaacg cggtgctcga tgcgccgcgc
     1441 cttcgacgat ccgcctggaa tgcggatcgc aacggtgcgg ttctcgtagc cccaggccgc
     1501 agcagagggc gcatgagtgc ccggcctgag tctacggtat gagttgaagt gcggtgcata
     1561 gagcagggtg gtctcgggca tggactcgat gagtccgccc acggcactgc gcatggtggt
     1621 gcttccgtca ttgctgccgt catcgaagac attcctgccc ttgctgtcga ggagcgaaaa
     1681 gtgaacatgc aggccactgc cggaacgctc gccataaggc ttcgccatga aagaggctgc
     1741 gaaaccatgc ttccgggcca ggccgcgcac gatccgcttg aacagcaccg cgtcgtcggc
     1801 ggcgcgcaac ggatcatcga catgcatgag gttgatctcg aattgtccga tgccgttttc
     1861 ggcaatggcg gagtcggcgg gcacaccctg ggctgcacag gcggcataga cctcctcgaa
     1921 gaagctgccg aagtcatcga gttcgtcgat cgacagaacg ccatcggcat caaggcgctt
     1981 gccggttacc ggtgacaccg gcgcgatcgg gcgcgcattg gtcggatcgt agagataaaa
     2041 ctccagttcc atggccatca cgggggtgag gccgtgtgcc ttgaagcggt ccacgagcgc
     2101 ggcgagcgcc cggcgtgggt caccgccgaa aggtactccg ctttcctcac gtagccagag
     2161 ggggatgagg cccgacggtt ctgccgtcca atccaccggc aggatgccgc gcccagtcga
     2221 ctcgcaagtt ccgtccgcat cgccggtggc aaagacgagc ggactcttga cgatatcctc
     2281 accccagatg tcgacgccga cgaccgacaa tggcatgcgc aacccgccgc tcaggacctt
     2341 ctccgcttgc tccagcggaa tgcgcttgcc acgcatgacg ccgttcaggt cgcagacggc
     2401 cgcgcgcagg ctcttgatgt cggtgcggct ttccagccag gccagtatct tgtccaagat
     2461 cggttgctct ccccaggttg cgggttttcc ttagcaatct tcgaggcaag gcgcaaaaag
     2521 ccacaatcaa gggattgcgc ccagcagggt gagaaccagc ggaatcgtga cggcggcaaa
     2581 gatcacttga acggtcagga tattggccac catcggggca tcaccgccca tctgccgggc
     2641 caggacatag gctcctgagc ccgtggggac agcggcgcag atcagcgcca cggcataggc
     2701 ttcgcccgtc acgccgaaga ggtgcagcat cacagccatg acgaccggca ataccagcag
     2761 cttgaggatg gctgcagcgg cgatcggcag ccagctggtg attgcttctc caaagcggag
     2821 gccggcgccg acggttagca gcgcgagccc cagcgcgcca cggccgatca tgtcgagcac
     2881 ctgaatgccc atctcatgca ccggcaggtc aagcacctgc caggcgactc caccaagtga
     2941 tgagaacaca aatggattgc ggatgatgga aagtgccagg gttccggcgc ttggccgatg
     3001 tgtatgggcg aagcgggcca tgacgacaac gcagacgatg ttgagcagcg gcgtcatcgt
     3061 cgcggcgctc acggcgccga gcgacagggc atcggcgccg aagatcaacg ggatgatggc
     3121 gaaggccacg aaggtgtgcc agcgcgttgc accctggaac aggctggtga actgcggccc
     3181 gtcgatcgac aacaccctca tcagtggtcg gttcaggatc agcagcgcgg caaacatcac
     3241 cgcgagcgcc aggaccatgc tgagcgccat gcccaggacc gggacgctgc tgaagtcggc
     3301 gcccgcgatc tccttgaaga tgatcgcggg aaaaaggacg aagtagcaga gccggtccaa
     3361 cgcttgccaa tggtcgtcga ccagcacgcc gaagcggcgc agcgcccagc cgatcagtat
     3421 gacgatgaag acgggcagca gg
//
LOCUS       DBNGVR010000034         3589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4334400, whole genome shotgun sequence.
ACCESSION   DBNGVR010000034 DBNGVR010000000
VERSION     DBNGVR010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4334400"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..2844
                     /locus_tag="AC16A0_00710"
     CDS             130..2844
                     /locus_tag="AC16A0_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR3103875.1"
                     /translation="MDKNLSKYIWKHTRSQQIWILIVVLVSMYTYFLSFDLPKFIVNG
                     PIQGQGFETPGATQTYLKLAPTLPFIGTIDIFPGFDLTRMGALIYLSLFFLLLVIVNG
                     AFKFYINTYKGRLGERMLRRIRFELVDRILRFPPQQFKYVKPAELATMVKDEVEPLGG
                     FIGDAFVQPALLGGQAATALIFIFVQNFWLGTIAAAIVAVQLIIIPKMRKRLLLLGRE
                     RQLTARELSGRISEISEGISTIHSHDTSNLERADISARLGRIFKIRYDLYQWKFLVKF
                     LNNFLAQVTPFLFYLIGGYQVISGTLDVGQLVAVIAAYKDLPSPLKDLIDWDQARQEV
                     KIKYLQVYEQFDIDNMMDGKIQALETKPVDPLNHALEAVNLSITDDSGARLLDRMSIS
                     VKHGESLAIVGNTGGSGEALTEALSRVIRPAGGKIALGPHDLHELPESVTGRRMSYAS
                     SDAYLFQGKLRDNLLYGLKHAPLQPPVERSEASAHKRWEIEEANKSGNVDYDIHADWI
                     DYAAAGATGPQDIVNVILPLLDAVQLSNELVELGLRSRTTASHHPKISEGIVAVRKAF
                     RERLASENLDEVVVPFKSGVYNPEATVSENLLFGAATGPLLDSSSLAKDAYFLSVLES
                     SGLTETFYKMGLEIAENVVELFRDLPPDHPFFQNLPFMTSDQLPEYQALLKRAQGKSF
                     PALTEADRTRVLALTFPYVEPQHRFGVLTPEIMARIVDMRHAFLRDLPDRLKGSIEPY
                     DPERYNTAASLLDNVLLGRIAHQHSDEGDHIRRIVREVLTEQGLREDVIDMGLAFNAG
                     VGGRRLSAGMRQKVNLARALIKRSDYLILNRPLSALDQQEQRSIAVNLLEWSRKMGYK
                     PAVVAVLSTPSLAALFDKVMVFERGAPVATGPYSKLVEENENFKKLLT"
     gene            2883..3353
                     /locus_tag="AC16A0_00715"
     CDS             2883..3353
                     /locus_tag="AC16A0_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3103876.1"
                     /translation="MALKEEVEQLKSVPLFADVSPAKLKLLAFTSDVVSFPAGRVLFN
                     QGDLGDAAYVVLSGEVDVLINAGGEQIKVDTIGANSIVGEIAILCDVVRTATIKTTQP
                     VTALRIRKEHFLKLLAEFPEMTIQIMRVLAERLNHTTSALTEARSQLRKAGQRA"
     gene            complement(3357..>3589)
                     /locus_tag="AC16A0_00720"
     CDS             complement(3357..>3589)
                     /locus_tag="AC16A0_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3103877.1"
                     /translation="IMNWRGNRGCRDHGELIDALSGLIHQHDRDETPIGILSHHLVHD
                     EAAWDFLRRLFRLTEGRWLSAADAINAVEAGR"
BASE COUNT          710 a         1094 c         1049 g          736 t
ORIGIN      
        1 ttggcgcgcc gttttcgtcg cgggtggtct gccggcgaac ggccggaaac tcgaaggccc
       61 cctgggtgcc cgggggacgc ggcgtattgc acgcttcggc agcctccgcg ttgcggcgag
      121 acttgcgtga tggacaagaa tctctcgaag tacatctgga agcatacacg cagtcagcag
      181 atctggatcc tgattgtcgt gcttgtctcg atgtacacct attttctgtc tttcgatctg
      241 ccgaagttca tcgtcaatgg tcccattcag ggccagggct tcgaaacgcc cggagcgacg
      301 cagacctatc tcaagctggc cccaaccctg ccattcatcg ggaccatcga catctttccg
      361 ggcttcgatc tcacccgcat gggcgcgctg atctatctga gcctcttctt cctgctgctc
      421 gtcatcgtga atggcgcctt caagttctac atcaatacct acaagggccg tctcggcgaa
      481 cgcatgctgc gccgcatccg cttcgaattg gttgaccgca tcctccgttt tccgccgcag
      541 cagttcaagt atgtgaagcc agccgagctt gccacgatgg tcaaggacga ggtggaacca
      601 cttggcggct tcatcggcga tgccttcgtg cagccggcct tgctgggcgg ccaggccgca
      661 acagcgttga tcttcatatt cgtgcagaat ttctggctcg gtacaatagc tgcggccatc
      721 gtggcagtgc agctcatcat cattccgaag atgcgcaagc gcctgctttt gctgggccgc
      781 gagcggcagc tgacggcccg cgaactctcc ggccgcatca gcgagatatc ggaaggcatc
      841 tcgaccatcc acagccacga tacgtcgaat ctcgagcgag ccgatatctc cgcgcggctg
      901 ggccggatct tcaagatccg ctacgatctt tatcaatgga agttcctggt gaagttcctg
      961 aacaacttcc tggcccaggt cacgcccttt cttttctacc tcatcggcgg ctaccaggtg
     1021 atcagcggca ccctcgacgt gggccagctg gtcgccgtca tcgccgccta caaggacctg
     1081 ccgagtccac tcaaggatct gatcgactgg gatcaggccc gccaggaagt gaagatcaag
     1141 tatctgcagg tgtacgagca gttcgacatc gataacatga tggacggcaa gattcaggcg
     1201 ctcgagacga agcccgtcga tccgctgaac catgcgcttg aagccgtcaa tctctcgatc
     1261 acggatgata gtggcgcaag acttctcgac cgcatgtcga tcagtgtgaa gcatggcgaa
     1321 tccctggcca tcgtcggcaa tacaggtggc agcggcgaag cgctgaccga ggcattgtcc
     1381 agggtcataa gacctgccgg aggcaagatc gcgctggggc cacatgacct tcacgagttg
     1441 cccgagtcgg tcaccggtcg ccgcatgtcc tatgcgagct ccgatgcgta tctctttcag
     1501 ggaaagctcc gcgacaatct cctgtatgga ttgaagcatg cgcctctcca gcctccggta
     1561 gagaggtccg aggcttcggc ccacaagaga tgggaaatcg aagaagccaa caaatcgggc
     1621 aatgtggact acgacatcca tgcggactgg atcgactatg cggcagcggg tgcaactgga
     1681 cctcaggaca tcgtcaatgt gattttgccg cttctggatg ccgtgcagct gtccaacgaa
     1741 cttgtcgagc tcggcctccg ctcgcggacc accgcaagcc atcacccgaa gatttccgaa
     1801 ggtatcgtcg ccgtgcggaa ggcattccgc gaacgcctcg catcggagaa ccttgatgag
     1861 gtcgtggtgc cgttcaagtc aggtgtctac aatccggagg caacagtcag cgagaacctg
     1921 ctgttcggtg cggcgaccgg tccgctactg gatagctcat ccctggccaa ggacgcctac
     1981 ttcctgtcgg tacttgagag ctcaggactg acagagacgt tctacaagat gggcctggag
     2041 attgccgaga acgtggtgga gcttttccgc gaccttccgc ctgaccatcc cttcttccag
     2101 aatcttccgt tcatgacgtc ggatcagctt cccgagtacc aggcgctcct gaagcgggcc
     2161 cagggcaaga gcttcccggc gctgaccgag gcggaccgga ccagggtcct ggctttgact
     2221 ttcccctacg tcgagcccca acaccgtttc ggcgtgctga cacccgaaat catggcgcgc
     2281 atcgtggaca tgcgtcacgc cttcctgagg gatctgccgg acaggctgaa gggcagcatc
     2341 gaaccctatg accccgaacg ctacaacacc gcggctagcc ttctcgacaa tgtgcttctc
     2401 ggccgtatcg cccaccagca ttccgatgag ggcgatcaca tccggcgtat cgtgcgcgag
     2461 gtcttgaccg agcaggggtt gcgcgaggac gtgatcgaca tggggctcgc cttcaatgcc
     2521 ggcgtgggtg gccgccgcct gtcggcagga atgcggcaga aggtcaacct tgcccgggcc
     2581 ctcatcaagc gctccgacta ccttatcctg aaccggcccc tgtcggcgct ggatcagcag
     2641 gaacagaggt cgattgccgt caatctgttg gagtggtcgc gcaagatggg ctacaagcct
     2701 gcagtcgttg ccgttctgtc gacaccgtcg cttgctgcgc ttttcgacaa ggtcatggtc
     2761 ttcgagcgcg gtgcacctgt cgcaaccggg ccttacagca aactcgtcga agagaacgag
     2821 aacttcaaga agctgcttac atgatgttca gtgtgtcaac gtcaagctag gggatccatg
     2881 caatggcgct caaggaagag gtcgagcagc tgaagagcgt tccgctcttc gcggatgtct
     2941 ctccggcgaa gctcaagctg ctcgccttca catccgatgt ggtcagtttt cctgccggac
     3001 gcgtgctctt caatcagggc gatctcggcg atgccgccta cgttgtcctc tccggagaag
     3061 tcgacgtgct catcaatgcg ggcggcgaac agatcaaggt tgacaccatt ggtgccaatt
     3121 cgatcgttgg agagatcgca atcctctgtg acgtggtcag gaccgcgacg atcaagacaa
     3181 ctcagccagt taccgcgctg cgtatccgca aggagcactt cctgaagctc ctggcggagt
     3241 tcccggagat gacgatccag atcatgcggg tactcgccga gcgtctgaac cataccacct
     3301 cggccctgac cgaggcgcgc agccagctgc gcaaggccgg tcagcgtgcc tgacgcctaa
     3361 cgtccggctt ccacggcatt gatcgcgtca gccgcgctga gccagcggcc ttcagtcagc
     3421 cggaaaaggc gccgcagaaa gtcccaggcg gcctcatcgt gaacaaggtg gtggctcagt
     3481 atgccaatcg gcgtttcatc acggtcgtgc tggtggatca ggccggagag cgcgtcgatc
     3541 agttcgccgt gatcgcggca gccgcggttg ccgcgccagt tcatgatgt
//
LOCUS       DBNGVR010000035         2491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_602988, whole genome shotgun sequence.
ACCESSION   DBNGVR010000035 DBNGVR010000000
VERSION     DBNGVR010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_602988"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1383
                     /gene="nuoF"
                     /locus_tag="AC16A0_00725"
     CDS             79..1383
                     /gene="nuoF"
                     /locus_tag="AC16A0_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3103878.1"
                     /translation="MLADKDRIFTNIYGFHDWGLEGAKKRGCWSGTKSFIANGRDWMI
                     NEMKASGLRGRGGAGFPTGLKWSFMPKVIGERPHYLVVNADESEPGTCKDREILRHDP
                     HLLVEGCLLAGAAMGAHVGYIYVRGEFIREREHLQAAIDQAYEARLLGKNNVNGWDFD
                     LYVHHGAGAYICGEETAMLESLEGKKGQPRMKPPFPANVGLYGAPTTINNVESIAVAG
                     TIMRRGATWFASLGRPNNTGTKLFCISGHVNRPCVVEEELGIPFRELIEKHAGGVRGG
                     FDNLLAVIPGGSSVRMVPAEQIIDTPMDFDSLSKLRSGLGTAAVIVMDKSTDLLKAIA
                     RLAYFYKHESCGQCTPCREGTGWMWRVMTRMAEGRAEKREIDMLLDVTKQIEGHTICA
                     LGDAAAWPIQGLIAHFRPVIEARIDQYAANPDHDGAVRHAAE"
     gene            1387..1794
                     /locus_tag="AC16A0_00730"
     CDS             1387..1794
                     /locus_tag="AC16A0_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103879.1"
                     /translation="MRAVNSSIGLTAAALAGLAFVLPAQALEIEQLLGRWSDPDLDEC
                     VYADDSEGAPLNIRREGDEIYVGNYGWLCSVPLAAWKKDGAFFIGEARDCGMEGGDDT
                     FDEVLVLGLNDKDQLLLTKDPAAKGLRRCPAAP"
     gene            1819..>2491
                     /locus_tag="AC16A0_00735"
     CDS             1819..>2491
                     /locus_tag="AC16A0_00735"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018447543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR3103880.1"
                     /translation="MPKIKIDGVELEVENGLTLLQACEQAGAEVPRFCYHERLSIAGN
                     CRMCLVEVKGGPPKPQASCAISVNDLRPGPNGEPPEVFTRSPMVKKAREGVMEFLLIN
                     HPLDCPICDQGGECDLQDQAMASGVSKNRFHENKRAVEDKYLGPLVKTSMNRCIQCTR
                     CVRFTTEVAGIEEMGATGRGEDMEITTYLNRAISSELQGNIIDLCPVGALTSKPYEFK
                     ARPWEL"
BASE COUNT          463 a          806 c          827 g          395 t
ORIGIN      
        1 caatgcaaga agctggcgaa gggctggcgt cccgacagcg atgtcggcga aaagccgaag
       61 aagaagtgag gctaggcgat gctcgctgac aaggaccgca tcttcacgaa tatctacggc
      121 ttccacgact ggggcctgga aggcgccaag aagcgcggct gctggtcggg cacgaaatcc
      181 ttcatcgcga atggccgcga ctggatgatc aacgagatga aggcatcggg cctgcgcggc
      241 cgcggcggcg cgggcttccc gaccggcctc aagtggtcct tcatgcccaa agtgatcggc
      301 gagcgcccgc attatctggt cgtcaatgcc gacgaatccg agcccggcac ctgcaaggac
      361 cgcgagatcc tgcgccacga tccgcatctg ctggtcgaag gctgcctgct ggccggtgcg
      421 gccatgggcg cccatgtcgg ctacatctat gtgcgcggcg agttcatccg cgagcgcgag
      481 cacctgcagg ccgccatcga ccaggcctac gaggcacgcc tcctcggcaa gaacaacgtc
      541 aacggctggg actttgacct ctacgtccat cacggcgccg gcgcctatat ctgcggcgag
      601 gagacggcca tgctggaaag cctggagggc aagaagggcc agccgcgcat gaagccgccg
      661 ttcccggcca atgtcgggct ctatggtgcg ccgaccacca tcaacaacgt cgagtcgatc
      721 gctgtggcgg gcaccatcat gcgccgcggt gccacctggt tcgcgtcgct cggccggccg
      781 aacaacaccg gtaccaagct cttctgcatc tcgggccacg tgaaccggcc ctgcgtggtg
      841 gaagaggaac ttggcattcc cttccgcgag ctgatcgaga agcacgccgg cggcgtgcgc
      901 ggcggcttcg acaatctgct ggccgtcatt cccggcggct catccgtgcg catggtgccg
      961 gcggagcaga tcatcgacac gccgatggat ttcgactcgc tctccaagct gcgctcgggt
     1021 ctcggcacgg cggcggtgat cgtgatggac aagtccaccg acctcctcaa ggccatcgcg
     1081 cgcctggctt acttctacaa gcacgagtcc tgcggccagt gcacgccctg ccgcgaaggc
     1141 acgggctgga tgtggcgggt gatgacgcgc atggccgagg gccgcgccga gaagcgcgag
     1201 atcgacatgc tgctcgacgt caccaagcag atcgagggac acacgatctg cgccctgggc
     1261 gatgccgcgg cctggccgat ccagggcctg atcgcgcatt tccgcccggt catcgaggcg
     1321 cgcatcgacc agtatgccgc caatcccgat catgacggcg ccgtgcgcca tgcggcggag
     1381 taggcgatgc gcgcggtgaa cagcagcatc ggcctcacgg cggcggccct ggccgggctg
     1441 gccttcgtgc tgccggcgca ggcgctggag atcgagcagc tgctcggccg ctggtccgac
     1501 ccggatcttg acgaatgcgt ctatgccgac gacagcgagg gagcaccgct gaacatccgc
     1561 cgcgagggcg acgagatcta tgtcggaaac tacggctggc tgtgttccgt cccgctggcg
     1621 gcgtggaaga aggatggcgc gttcttcatt ggcgaagcca gggactgcgg catggagggc
     1681 ggcgacgaca ccttcgacga ggtgctggtt ctcggcctga acgacaagga ccagctcctg
     1741 ttgacaaagg acccggcggc caaaggattg cggcgctgcc cggcggcgcc atgagagatg
     1801 tgatgagcga gcacgctgat gcctaagatc aagatcgatg gagtggaact cgaggtcgag
     1861 aacgggctga ccctgttgca ggcctgcgag caggccggcg cagaggtgcc gcgcttctgc
     1921 tatcacgagc gcctctcgat cgccggcaat tgccgcatgt gcctggtcga ggtgaagggc
     1981 ggaccgccca agccgcaggc ctcctgcgcc atttccgtca acgacctgcg ccccggcccc
     2041 aatggcgagc cgccggaagt cttcacccgc tcgcccatgg tgaagaaggc gcgcgaaggc
     2101 gtgatggaat tcctgctcat caaccatccg cttgactgcc ccatctgcga ccagggcggc
     2161 gaatgcgacc tgcaggacca ggccatggcc tctggcgtgt ccaagaaccg cttccatgag
     2221 aacaagcggg ccgtcgagga caagtacctg ggcccgctgg tcaagacctc gatgaaccgc
     2281 tgcatccagt gcacgcgctg cgtccgcttc accaccgaag tcgctggcat cgaggagatg
     2341 ggtgcgacgg gccgtggcga ggacatggag atcaccacct acctgaaccg cgcgatctcg
     2401 tcggagcttc agggcaacat catcgacctt tgcccggtcg gcgcgctgac ctccaagccc
     2461 tatgaattca aggcgcggcc gtgggagctt c
//
LOCUS       DBNGVR010000036         3814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_406761, whole genome shotgun sequence.
ACCESSION   DBNGVR010000036 DBNGVR010000000
VERSION     DBNGVR010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_406761"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="AC16A0_00740"
     CDS             complement(<1..90)
                     /locus_tag="AC16A0_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3103881.1"
                     /translation="MKNENALNALSALAQPTRLDVFRLLVKQEP"
     gene            332..823
                     /locus_tag="AC16A0_00745"
     CDS             332..823
                     /locus_tag="AC16A0_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HIR3103882.1"
                     /translation="MLKAVAVLPAGKRATVAETAGTAVLAHDERHLRRKAITLSDGGR
                     ILVDLPEPVMLSAGDGLVLEDGRHVVIAAAVEDLLEIVAHNPLHLCELAWHIGNRHLP
                     AAIEADRILILRDHVIADMLRGLGAELREIAAPFTPMRGAYSGNGAHHHEERQYQLHA
                     AQT"
     gene            848..1492
                     /gene="ureG"
                     /locus_tag="AC16A0_00750"
     CDS             848..1492
                     /gene="ureG"
                     /locus_tag="AC16A0_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HIR3103883.1"
                     /translation="MTSMNGPLRIGIGGPVGSGKTTLTEKLCKAMRNDYSIAVITNDI
                     YTKEDAQILARLQALPEDRIMGVETGGCPHTAIREDASINLQAVAEMSRRFPDLDVIF
                     IESGGDNLAATFSPDLADLTLYVISVCQGEEIPRKGGPGISRSDFLVINKSDLAPYVN
                     VNLGVMERDAARMRGGRPFGFTDLSRGKGLAEVIGFIAENGGLAKATSPARPAA"
     gene            1506..3197
                     /locus_tag="AC16A0_00755"
     CDS             1506..3197
                     /locus_tag="AC16A0_00755"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3103884.1"
                     /translation="MTEQAGAVPGPSVSTPSIGIRELRLVSGLVLGCLLTTHFLNHAL
                     GLVSLSAMESGRGWFNLLWRSMPGTILLYGAILIHFALALLALYRRRTLRMPAREAFQ
                     LGFGLLLPFLLIPHVTGTRIVLAVWGIDFDYARVMQNISASPDGMTRQTAALLIAWVH
                     FGLGIHFWLRARPWYPRVALLLYSAALLVPVFALLGLAEAGKDLGDAVVASGPPVPVP
                     DPATLGQIRNGLYLTFAGLIAATLAARALRASHVFATRIDVTYPNGQMVSVPRGHSVL
                     EASRIGGIPHMSVCGGRGRCSTCRLRVLQGLEKQPPPSAQERATLKRIKATPNVRLAC
                     QLRPADHLSVAPLLVLDERKSSAQFGRSLAQGGRECEIAVLFCDLRGFTSLAEHRLPY
                     DTVFILNRFFDVVGHAVEESGGHVDKFIGDGALALFGLDGDEGSAARQALAAARLIGR
                     GVAGLNASLGSELERPLRIAMGLHAGHAIVGEMGYGKATTLTAIGDSLNTASRLEGFA
                     KESDAEVVVSDDLMRRAGFAVSGAERQTVMVRGRSTPIVTWIIRDAASIAAPPMA"
     gene            3259..>3814
                     /locus_tag="AC16A0_00760"
     CDS             3259..>3814
                     /locus_tag="AC16A0_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003589632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HIR3103885.1"
                     /translation="MMLPVGVAIDPKSGAMGRATGRYVKRLGETRGCYHDQAAVDALL
                     AARGDVPVYEVVEFIQPGSDIIFGTTTMSPGQVGEEFFMTRGHFHQRRDRGEVYYTQS
                     GKGLLLLESRHGESRTVDMVPGTCAFIPPDWAHRSVNTGDAPLVFVWVCNADAGHDYA
                     EILTRGMRQMVVKRDGAVALAPNPR"
BASE COUNT          619 a         1224 c         1226 g          745 t
ORIGIN      
        1 cggctcttgc tttacaagca accggaagac atccaaccgg gtcggttgcg ccagggcgga
       61 cagggcattg agagcattct cgtttttcat gtgtcgagac gtatcgacat tatatgtgac
      121 gatcaagtga cagttccgat tcgccgccaa agaattccat ttccgcgccg attcagtaat
      181 ttagtgcaat tggttacgtg gtccgggctg gcgtcgcggc gcggggcagc ctggcgtccc
      241 cggaatcgcc ggaagggaac tggcggcgtt gacttgttcc ggcaggttgt ggagagaatg
      301 ccatgtcaat ggcaatcggg ggttcatgca catgctcaag gcggtcgccg tgttgccggc
      361 cgggaaacgg gcgaccgtcg cggaaacagc cggtaccgcc gttctcgccc atgacgagcg
      421 gcatctgcgg cgcaaggcga tcaccctttc cgatggcggc aggattctgg tcgatctccc
      481 ggaaccggtg atgctgtctg ccggtgatgg tctggtgctt gaagacgggc ggcatgtggt
      541 gatcgccgct gccgtggaag acctgctcga gatcgtcgcg cacaatccgc tgcatctctg
      601 cgaactcgcc tggcacatcg gcaaccgcca tctcccggcg gccatcgagg ctgaccgcat
      661 cctgatcttg cgcgatcatg tgattgccga catgctgcgc gggctgggtg ccgagctgcg
      721 cgagatcgcg gcacccttca cgccaatgcg cggtgcctac tccggcaatg gcgcgcatca
      781 ccatgaagaa aggcaatacc agctccatgc cgctcagacg tgacgcagaa atgaggacct
      841 caggccaatg acatcaatga acggtccgct tcgcatcggc atcggcggtc ctgtcgggtc
      901 cggcaagacc acgctcaccg agaagctctg caaggcgatg cgcaatgact attccatcgc
      961 cgtcatcacc aacgacatct ataccaagga agatgcgcag attctcgcgc gcctgcaggc
     1021 gctgccggaa gaccggatca tgggcgtgga aacggggggc tgtccgcaca cggccattcg
     1081 ggaggacgct tcgatcaatc ttcaggctgt cgccgaaatg agccgcagat ttccagatct
     1141 tgacgtgatc ttcatcgagt cgggcggcga caatctggct gccacctttt cgcccgacct
     1201 ggccgacctt acgctgtatg tcatttccgt ctgccagggc gaggagattc cgcgcaaggg
     1261 cggaccgggc atcagccgct ccgatttcct ggtcatcaac aagagtgacc ttgcgcccta
     1321 tgtgaacgtg aatctcggcg tcatggagag agatgccgcc cgcatgcgcg gcggccgacc
     1381 cttcggtttc accgacttgt cgcggggcaa gggtctggcc gaagtgatcg gcttcatcgc
     1441 cgagaacggc gggctggcaa aggcaacttc acccgcgcgt cctgcggcgt gacagcattc
     1501 aggtcatgac cgagcaggcc ggggccgttc cggggccttc cgtctccacg ccgtccatcg
     1561 gcatacgtga gcttcggctg gtgtccggac tcgttctcgg ctgtcttctc accacgcatt
     1621 ttctcaatca tgccctgggg ctcgtctcgc tttccgcgat ggagtccggg cgcggatggt
     1681 tcaatctcct gtggcgcagc atgccgggaa ccatcctgct ctacggcgcc atcctgatcc
     1741 acttcgcgct ggcgcttctg gcgctctacc ggcgccgcac gctccgcatg cccgcccgcg
     1801 aggcattcca gctgggtttt ggattgctgc ttccctttct gctcattccg catgtcaccg
     1861 gcacgcggat cgtcctggcg gtctggggca tcgacttcga ctatgccaga gtcatgcaga
     1921 acatctcggc gtcgccggac ggcatgaccc ggcagacggc ggctctgctg attgcctggg
     1981 tgcatttcgg cttgggtatc cacttctggc ttcgcgccag gccctggtat ccccgggtcg
     2041 ctctgctgct ctattccgcc gcgctgctgg tgccggtctt cgctctgctc ggtctggccg
     2101 aggcaggcaa ggatctgggc gacgctgtgg tcgcaagtgg tccgcctgtg cctgtgccgg
     2161 atcccgccac gctcggacag atccgcaatg gcctctatct cacctttgct gggctgatcg
     2221 ctgcaacgct ggctgcacgc gcgctgcgag cgtcgcatgt cttcgccacg cgcatcgatg
     2281 tcacatatcc caacggacag atggtcagcg tgccgcgcgg gcacagcgtg ctcgaggcca
     2341 gccgcattgg cggaattccg cacatgtcgg tctgtggcgg gcgcggacgc tgctcaacct
     2401 gccggctccg cgtgctccag ggtctggaga agcagccgcc gccgtctgcg caggaacgcg
     2461 ccacgctcaa gcggatcaag gccacgccca atgtccgcct cgcctgccag ctgcggcccg
     2521 ccgatcatct ttccgttgcg cccctgcttg tcctggacga gcgcaagtca tccgcgcagt
     2581 tcggcaggag cctggcgcag ggcgggcgcg agtgcgagat cgcggtcctc ttctgcgacc
     2641 tgcgcggctt cacgtctctt gccgagcacc gcttgcccta cgacacggtc ttcatcctta
     2701 accgcttctt cgacgtggtg ggtcacgcgg tggaagaatc gggcggccat gtcgacaagt
     2761 tcatcggcga cggcgcactg gccttgttcg gacttgatgg cgacgaggga tcggcggcgc
     2821 ggcaggcgct ggccgcggcc aggctcatcg gcaggggcgt ggcagggctc aatgcatcgc
     2881 tgggcagcga gctggaacgg ccgctgcgca tcgccatggg attgcacgcg ggccatgcga
     2941 tcgtgggcga gatgggttat ggcaaggcca ccacgctgac ggcgatcggc gattcgctca
     3001 acaccgccag ccgcctcgaa ggcttcgcca aggaatccga tgcggaggtc gtggtctccg
     3061 atgacctgat gcgccgcgca ggcttcgccg tttcgggtgc ggagcgccag accgtcatgg
     3121 tgcgtggccg cagcacgccg atcgtcacct ggatcatccg cgacgccgca agcatcgccg
     3181 cgccgccgat ggcttgacag ctgcgagacg ccgtcgttac ctcgcgcctt cgacgtgttc
     3241 aatcacattg gtgcagacat gatgcttccc gtcggcgttg cgatcgatcc gaagagcggg
     3301 gccatgggcc gggccaccgg ccgctatgtc aagcgcctcg gcgagacacg tggttgctac
     3361 catgatcagg ctgccgttga tgcgctgctc gcggcgcgcg gtgacgtccc ggtttacgaa
     3421 gtggtcgagt tcatccagcc gggctcggac atcatcttcg gaaccacaac catgtcgccg
     3481 gggcaggtgg gcgaggagtt tttcatgacc cgtggccact tccaccagcg ccgcgaccgg
     3541 ggcgaagtct actatactca gtcgggcaag ggactgctcc tgctcgaatc gcggcatggc
     3601 gaatcccgca ctgtcgacat ggtgccgggg acctgcgcct tcatcccgcc ggactgggcg
     3661 caccgctcgg tcaacaccgg cgacgcgccg cttgtgttcg tctgggtctg caatgcggat
     3721 gccggccacg attatgcgga gatcctgacg cgcggcatgc gccagatggt ggtgaagcgc
     3781 gatggcgcgg tggcgcttgc gcccaatccg cgct
//
LOCUS       DBNGVR010000037         2180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4335686, whole genome shotgun sequence.
ACCESSION   DBNGVR010000037 DBNGVR010000000
VERSION     DBNGVR010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4335686"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16A0_00765"
     CDS             <1..237
                     /locus_tag="AC16A0_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HIR3103886.1"
                     /translation="VLAVDDVAFGEKLAFETCYVVGCLVRLQFDASSLEKLEKGTSLT
                     ISAIAAETDQPIKFGISLKGFSRALQRLRSLLQP"
     gene            complement(387..1826)
                     /locus_tag="AC16A0_00770"
     CDS             complement(387..1826)
                     /locus_tag="AC16A0_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HIR3103887.1"
                     /translation="MTFSRRDVSKTLLGLAGSLMLASPALAFDGPFADVLEGGEDFKT
                     AIEAYTFGYPLVTMEMTRRVITNVAEPAGTRGPMGQIIKLREYPNASFRDVTAPNADT
                     LYTTSFFDVTKEPWVLSIPDMKDRYALFPMLDGWTEVFEVPGKRTTGTAAQTYAITGP
                     GWSGTLPEGVTEYKSPTGIVWLLGRIYCTGTPEDYAAVHALQDQFKLVPLSAYGTDWT
                     PPPGTVDPSIDMKTAVREQVNRMDAVEYFTLLCELMKTNPPKPADAPALAQFAQIGIV
                     PGQSFDASKFDPAFAKRIPEVAFARIMLHFKFSDGDVQNINGWGFTTKTGLYGTNYIQ
                     RALITAIGLGANRPEDAIYPTSPKPPGLLHRSYQGSENYTLTFEKDLLPPVNGFWSLT
                     MYDKNYFFVDNPLNRYSISPRQDLKPNADGSVTLYIQNQSPGADKESNWLPAPKDKFI
                     LMMRLYWPKEASPTILNGSWSIPEVKKVG"
BASE COUNT          373 a          644 c          732 g          431 t
ORIGIN      
        1 gtgctggccg ttgatgacgt ggcattcggg gagaagctcg ccttcgagac ctgttacgtt
       61 gtgggttgcc tggtcaggct ccagttcgat gcatcgagtc tggagaagct ggagaagggg
      121 accagtctca ccatctctgc aattgccgcg gaaaccgatc agcccatcaa gttcggcatt
      181 tcgctgaagg gcttctcccg ggcattgcag cggctgcggt cgctgctgca gccgtagcgt
      241 ctggcggtcc tggcctcggc gcccgatgac gtcctgccgg gcgccgggga gaccgcaaag
      301 gaacgaaggc cggcgaaggc ctgccgcgca gtccctcagt tcgccggcgc gatcatcgat
      361 ccgccagttg caaccgcggt cgcggctcag ccgaccttct tcacctcggg aatgctccac
      421 gacccgttga ggatggtcgg cgaggcctcc ttcggccagt acaggcgcat catgaggatg
      481 aacttgtcct tcggcgccgg cagccagttg ctttccttgt cggcccccgg cgactggttc
      541 tggatgtaga gggtcacgga accgtccgca ttgggcttga ggtcctggcg cgggctgatc
      601 gaatagcggt tgagcggatt gtcgacgaag aaatagttct tgtcatacat ggtgagcgac
      661 cagaagccgt tgaccggcgg cagcagatcc ttctcgaagg tcagcgtata gttctccgag
      721 ccttggtagg acctgtgcag cagtccgggc ggcttcggcg aggtcggata gatcgcgtct
      781 tccggccggt tggcgccgag gccgatggcc gtgatgagcg cgcgctggat gtagttggtg
      841 ccgtagaggc ccgtcttggt ggtgaagccc cagccgttga tgttctggac gtcgccgtcg
      901 ctgaatttga aatgcagcat gatgcgggcg aaggccactt ccgggatgcg cttggcgaag
      961 gccggatcga acttgctggc atcgaagctt tgtccgggaa cgatgccgat ctgggcgaac
     1021 tgcgccagcg ccggcgcatc ggcgggtttc ggcggattgg tcttcatgag ctcgcagaga
     1081 agggtgaaat actcgaccgc atccatccgg ttgacctgtt cgcgcaccgc cgtcttcatg
     1141 tcgatcgagg gatcgaccgt gccgggcggc ggcgtccagt cggtgccgta ggcgctcagc
     1201 ggcaccagct tgaactggtc ctgcagggca tgcacggccg catagtcctc cggcgtgccg
     1261 gtgcagtaga tgcggcccag cagccacacg atgccggtcg gcgacttgta ctcggtgacg
     1321 ccctccggaa gcgttccgct ccatccgggt ccggtgatgg cataggtctg cgccgccgtg
     1381 cccgtcgtgc gcttgcccgg aacctcgaag acctcggtcc agccgtcgag catcggaaac
     1441 agcgcgtagc ggtccttcat gtccgggatg ctgagaaccc agggctcctt ggtgacatcg
     1501 aaaaacgatg tcgtgtagag cgtatcggca ttgggcgcgg tcacgtcgcg gaagctggcg
     1561 ttgggatatt cgcgaagctt gatgatctgt cccatgggcc cgcgcgtgcc cgccggttcg
     1621 gcgacattgg tgatgacgcg ccgcgtcatc tccatggtca caagcggata gccgaaggta
     1681 taggcctcga tcgcggtctt gaagtcttcg ccgccttcca ggacgtcggc gaatggtcca
     1741 tcgaaggcca atgcggggct cgcgagcatc aacgagccgg caaggccaag cagtgtcttc
     1801 gaaacgtcgc ggcgggaaaa ggtcatgaag cagtctcctg attccggatg aatggcacgg
     1861 tcgatcgcgg gctgatcttc ccgcgcatga cgacgatcgg acttaccata agtcggcaaa
     1921 gtggatttga cttttggtgc caactatact ccgctcggat tctggcagaa acttggaacc
     1981 gagggctccg catgagtctc gacacgacga cgttccgcat gggtccaagc cagggatttc
     2041 cggcagtcat ccgctagctg ggtcatgacc cgcagccgct cctcgcggtg gcgggactgg
     2101 acgagcgcct attcgctcat gccgagaacc gcggcgaggc gcgcgtcatc ggccgcttcg
     2161 cgcatctggc ggcgcgccag
//
LOCUS       DBNGVR010000038         1514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3943256, whole genome shotgun sequence.
ACCESSION   DBNGVR010000038 DBNGVR010000000
VERSION     DBNGVR010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3943256"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="AC16A0_00775"
     CDS             <1..369
                     /locus_tag="AC16A0_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103888.1"
                     /translation="QGEYNIVEQVLGGTAVQLDPNNILVDKVRRILFGKGVSAAAIIH
                     VRRALDAAGYRQATIVGSSGFDPQKCQIMGAARVPLDVVGTGSFLPATLTETYATADI
                     IAYDGVKRVKVGREFLYDDK"
     gene            416..871
                     /locus_tag="AC16A0_00780"
     CDS             416..871
                     /locus_tag="AC16A0_00780"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3103889.1"
                     /translation="MMNRLGTFLIGILVAGFMLTGIGDANTGSKKGDVVEIRIDISSQ
                     TMAVDVNGWPYGRWKVSTARSGYHTPRGAWRPYSLKKMHYSRKYDNSPMPHSIFFLGG
                     YAIHGTGYVKSLGRPASHGCIRLHPQNASRLFNLVRKHGMKSTRILITN"
     gene            882..1469
                     /locus_tag="AC16A0_00785"
     CDS             882..1469
                     /locus_tag="AC16A0_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018446953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HIR3103890.1"
                     /translation="MTRPAALQQFLDAAAAAVSNAATPGSPADQAAGRVFSRARSSLG
                     SAGAAKAERLPVCRHLDAAFEGTKGKTVSAVAAALQQIEPAFRWTRRSTADPHDAVFW
                     TGHANAVILGPGGIEERSDLWIGASLMAPHVTYVDHDHPPEEVYLALTPGEWWNADMD
                     WTDPGAGGLIYNRPGIRHAMRSGATPFLALWFLPI"
BASE COUNT          265 a          481 c          487 g          281 t
ORIGIN      
        1 cagggcgagt acaatatcgt cgaacaggtc ttgggcggca cggcggtcca gctcgacccc
       61 aacaacatcc tggtcgacaa ggttcgccgc attctcttcg gcaagggcgt gtccgcggct
      121 gccatcatcc atgtcaggcg ggcgctggat gctgccggat accgccaggc caccatcgtc
      181 ggctcctcgg ggttcgatcc gcagaagtgc cagatcatgg gggccgcgcg cgtcccgctg
      241 gacgtggtcg ggacgggcag ctttttgcct gcgacgttaa ccgagaccta tgcgacagct
      301 gatataattg cttatgatgg cgtgaagcgc gtgaaggtcg ggcgggagtt cctctacgac
      361 gacaagtagc ggcgaaacct tgaagctgtg agagggggct ctcgggagcg aggttatgat
      421 gaacaggctg ggaacatttc tgatcgggat tctggtcgcc ggtttcatgc tgaccggcat
      481 cggcgacgcc aataccggtt cgaagaaggg ggatgtggtt gaaatccgca tcgacatttc
      541 aagccagacc atggcggtgg atgtgaatgg ctggccctat ggccgctgga aggtgtcgac
      601 ggcacgctcg ggctatcaca cgccgcgcgg tgcctggcgg ccctattcgc tgaagaagat
      661 gcattactcg cggaagtacg acaattcgcc gatgccgcat tccatcttct ttctcggcgg
      721 ctatgccatt cacgggacgg gatacgtcaa gtcgctcggc cgtccggcat cgcacggctg
      781 catccgtctg catccgcaga atgcgtcgcg gctcttcaat ctcgtcagga agcacgggat
      841 gaagtcgact cgcatcctca tcaccaactg accggtctcg catgacgcgt ccggccgcgc
      901 tgcagcaatt cctggacgct gcagccgccg ccgtgtccaa tgccgcgacg ccagggtcac
      961 cagcggacca ggcggcgggc agggtcttca gccgggcgcg gtcgagcctt gggtctgctg
     1021 gcgcggccaa ggccgaacgc cttccggtct gccgccatct cgacgcagct tttgagggta
     1081 caaagggcaa gacagtttcg gcggtggccg ctgccctcca acagatcgaa cccgcctttc
     1141 gctggacccg ccgatccacc gccgatccgc acgacgcggt cttctggact ggtcacgcca
     1201 atgccgtgat cctgggcccc ggcggcatcg aggagcgcag cgacctctgg atcggcgcca
     1261 gcctgatggc gccgcacgtc acctacgtgg atcacgatca tccgccggaa gaagtctacc
     1321 tcgcgctgac gcccggagaa tggtggaacg ccgacatgga ctggaccgat cccggtgcgg
     1381 gtggcctgat ctacaaccgg cccggcattc gccacgccat gcggtcgggt gccacgccgt
     1441 tcctcgccct gtggttcctg ccgatctgac cttcgccggc cctccggccg atgtcccggc
     1501 gagttggcag cagt
//
LOCUS       DBNGVR010000039         2034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3354007, whole genome shotgun sequence.
ACCESSION   DBNGVR010000039 DBNGVR010000000
VERSION     DBNGVR010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3354007"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC16A0_00790"
     CDS             <1..357
                     /locus_tag="AC16A0_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR3103891.1"
                     /translation="REFDPQPFHLDEQAAQQSVLKGLSASGWQSSAILVRLAVDAYAN
                     RSAALGSKGFDEAKWLKPVYAGETLTGRVTVLSKRRSASKPGIGVMKCLFELLNTAGE
                     KKVEMTGVQFMKLRHP"
     gene            354..842
                     /locus_tag="AC16A0_00795"
     CDS             354..842
                     /locus_tag="AC16A0_00795"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3103892.1"
                     /translation="MIGLYLDELEIGRVIPLGTHHFTRDAIVDFARTYDPQPFHLDEE
                     AGRKGPFGVLSASGWHTAAGWMKCYVATNQAAEAKLRAAGKTPAPLGPSPGFENLVWH
                     KPVKPGDTITYTLTVTGKRGLASRPGWGLLFSHSTGVNQDGDEVFAFDGKVMTPVRPV
                     SG"
     gene            complement(839..1384)
                     /locus_tag="AC16A0_00800"
     CDS             complement(839..1384)
                     /locus_tag="AC16A0_00800"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR3103893.1"
                     /translation="MDLRNGFDIKRYIRTIPDYPKPGIMFRDITTLLSNLHAFRAAVD
                     ELAWPFLRGEIDYVAGIEARGFILGGAVAHNLGRGFIPIRKKGKLPSRVIGQDYALEY
                     GVDTIEIHADAIQKGDRILLIDDLIATGGTAGAAIDLIRKSGGEVVAAAFVIDLPELG
                     GAAKLKAKGVKVHTLVSFEGH"
     gene            complement(1397..>2034)
                     /gene="hemF"
                     /locus_tag="AC16A0_00805"
     CDS             complement(1397..>2034)
                     /gene="hemF"
                     /locus_tag="AC16A0_00805"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HIR3103894.1"
                     /translation="TVHGEFSPEFRKQIPGAETDPRFWASGISLIIHPWNPHVPAVHM
                     NTRFIVTTKTWFGGGADLTPVLDRYRTQDYADTLDFHAAMKAACDAHAVADYTRFKTW
                     CDEYFFLPHRNEPRGIGGIFYDHFNSGNWDADFAFTQDVGRAFLGIYPKLVRRSMDKD
                     YGEADREEQLIRRGRYVEYNLLYDRGTIFGLKTGGNVESILSSMPPVVKWP"
BASE COUNT          358 a          632 c          669 g          375 t
ORIGIN      
        1 cgcgaatttg atccgcagcc ctttcacctt gatgagcagg cggcgcagca gtcggtgctg
       61 aaggggctct ccgcctcggg ctggcagagc agcgccatcc tcgtccgtct tgccgtggat
      121 gcctatgcca acaggtcggc cgctctaggc tccaagggtt tcgacgaggc gaagtggctg
      181 aagccggtct atgccggcga gacgctgacc ggacgggtga ccgtcctgtc caagcggcgc
      241 tcggccagca agccgggaat cggcgtcatg aagtgcttgt tcgaactgct caacacggcg
      301 ggcgagaaga aagtcgagat gaccggcgtt cagttcatga agctgcgcca cccatgatcg
      361 gcctctatct cgacgagttg gagattggcc gggtcatccc gctcggcacc caccatttca
      421 cccgcgatgc catcgtcgac ttcgccagga catacgatcc gcagcccttc catctcgacg
      481 aggaggccgg ccgaaaggga cccttcggcg tgctctccgc ttcgggctgg cacacggcgg
      541 cgggctggat gaagtgttat gtcgcgacca accaggcagc agaggcgaag ctccgcgccg
      601 cgggcaaaac gcccgcgcct ctcggtcctt cgccgggctt cgagaatctc gtctggcaca
      661 agccggtgaa gccaggtgac acgatcacct ataccctcac cgtcaccggc aagcgtgggt
      721 tggcgtcgcg gccgggctgg ggccttctct tctcgcacag cacaggcgtg aaccaggacg
      781 gcgacgaggt cttcgccttc gacggcaagg tgatgacgcc tgtgaggcca gtctccggct
      841 aatgcccctc gaagctcacc agcgtgtgca ccttcacgcc cttggccttg agcttcgcag
      901 cgccgcccag ttccggcagg tcgatcacga aggcggctgc taccacttcg ccgcctgact
      961 tgcggatcag gtcgatggcg gcgcccgcgg ttccgccggt ggcgatgaga tcgtcgatca
     1021 gcaggatgcg atcgcccttc tgaatcgcat cggcatggat ctcgatggtg tcgacgccgt
     1081 actccagcgc atagtcctgg ccgatcaccc gcgacggcag cttgcctttc ttgcggatgg
     1141 ggatgaagcc gcgcccgaga ttatgcgcca cggcgccgcc aaggatgaac ccgcgcgcct
     1201 cgatcccggc cacgtagtcg atctcgccgc gcaggaaggg ccaggccagt tcgtcgacgg
     1261 cggcgcggaa cgcatgcagg ttggacagca gcgtggtgat atcgcggaac atgatccccg
     1321 gcttgggata gtcgggaatg gtgcggatgt agcgcttgat gtcgaagccg ttgcgaaggt
     1381 ccatggcgca ttctcctcat ggccacttta cgacaggcgg catggaggac aagatcgact
     1441 ccacattgcc gccggtcttg agtccgaaga tcgtgccgcg gtcgtagagc aggttgtatt
     1501 ccacgtagcg cccgcgccgg atcagttgct cttcgcgatc cgcttcgccg tagtccttgt
     1561 ccatgctgcg ccggaccagc ttcggataga tgccgaggaa ggcacggccc acgtcctggg
     1621 taaaggcgaa gtcggcgtcc cagttgcccg agttgaagtg atcatagaag atgccgccga
     1681 tgccgcgcgg ctcgttgcga tggggcagga agaaatactc gtcgcaccat gtcttgaacc
     1741 gcgtgtagtc ggccaccgca tgggcgtcgc aggcggcctt catcgccgca tggaaatcca
     1801 gggtgtcggc atagtcctgg gtgcggtagc ggtcgagcac gggggtcagg tcggcgccgc
     1861 cgccgaacca cgttttggtg gtcacgatga agcgtgtgtt catgtggacc gccggtacat
     1921 gcgggttcca cggatggatg atgagcgaaa tccccgaggc ccagaagcgc ggatcggttt
     1981 cggcacccgg gatctgcttg cggaattcgg ggctgaactc gccatgcacg gtcg
//
LOCUS       DBNGVR010000040         2391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3944186, whole genome shotgun sequence.
ACCESSION   DBNGVR010000040 DBNGVR010000000
VERSION     DBNGVR010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3944186"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..2060
                     /locus_tag="AC16A0_00810"
     CDS             264..2060
                     /locus_tag="AC16A0_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3103895.1"
                     /translation="MAKASPTQAVKAWLKRFDAALTAGDVDAAVKLFGAESFWRDLVS
                     FTWNIKTSEGQQQIADMLKATLKKVKPSNWQIEGEASEGGGLQEAWITFETKVARGRG
                     HLRLKDGKAWTLLTTMVELKGHEEPSGEKRPPGVVHGAFRDRKTWAERRAEEQAKLGY
                     EEQPYVLIVGGGQGAIGLGARLKRLGVPTLIVEKNERPGDSWRKRYKSLCLHDPVWYD
                     HMPYLPFPDHWPVFSPKDKIGDWLEMYTKVMELNYWGSTVCEKASYDPEKKEWTVEVV
                     RNGQPVTLRPKQLVLATGMSSFPNIPKDPGHETFLGEIQHSSQHPGPDAYAGKKVVVI
                     GSNNSAHDICAALWEVGADVTMVQRSTTHIVKSDTLMDIGLSALYSEKAVKSGIDHHK
                     ADLIFASVPYRVLPAIQTPLYEQMKKKDAGFYKRLEKAGFMLDWGEDGSGLFMKYLRR
                     GSGYYIDVGASELVCDGKIKLKSGVSVKEIRPHSIVFSDGSELEADLIIYATGYSSMN
                     GWAAKLISQEVADKVGKVWGLGSGTKKDPGPWEGELRNMWKPTQQEALWFHGGNLHQS
                     RHYSQFLSLQLKARQEGIPTPVYGLPEVHHKA"
     gene            complement(2115..>2391)
                     /locus_tag="AC16A0_00815"
     CDS             complement(2115..>2391)
                     /locus_tag="AC16A0_00815"
                     /inference="COORDINATES: protein motif:HMM:NF016819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR3103896.1"
                     /translation="GLPYVEAGILLSVGILGLAALIRRSIPLVVATMLAGAFAVFHGY
                     AHTVEMPIGASALDYALGFIAGTVILHGAGLGAGLALNRVMPVRRTA"
BASE COUNT          497 a          733 c          797 g          364 t
ORIGIN      
        1 caccgcgatg cgcttgtgcg gggcagcatg gccgatgagg ccgcatccat tcacgactga
       61 catgggcttc gcgcggcaga gcccattgtc atcggcgcgg aaatgatcat aactttgcgc
      121 aaaacaaaat cgaaccggga ggacccagga tggccaagac cgccgcaaag aagaaagccg
      181 cgaagaaatc cgccgtgaaa cctgccaaga agtcagcgtc acgcaaggcc gcaaagccgg
      241 caccgcgcaa ggcggcgaag gcggtggcga aggcctcgcc gacccaggcg gtgaaagcgt
      301 ggctgaagcg cttcgacgcg gcgctgaccg cgggcgatgt cgatgctgcg gtcaagctct
      361 tcggtgcgga gagtttctgg cgcgatctgg tgtccttcac ctggaacatc aagacctccg
      421 aaggccagca acagatcgcc gacatgctca aggcgacgct caagaaggtg aagccgtcaa
      481 actggcagat cgaaggcgag gccagcgaag gcggcggcct gcaggaggcc tggatcacat
      541 tcgagacaaa ggtggcgcgc ggccgcgggc atctgcgcct caaggacggc aaggcctgga
      601 cgctgctcac caccatggtg gaactcaagg gccatgaaga gccctctggc gagaagcgcc
      661 cgccgggcgt cgtccatggc gccttcaggg accgcaagac gtgggccgag cgccgcgccg
      721 aggaacaggc gaagctcggc tatgaggagc agccctatgt gctcatcgtc ggcggcggac
      781 agggggcgat cgggcttggc gcgcggctga aacggctggg cgttccgacg ctcatcgtcg
      841 agaagaacga gaggccgggc gattcctggc gcaagcgcta caagtcgctc tgcctgcatg
      901 atccggtctg gtacgaccac atgccctatc tgcccttccc ggatcactgg ccggttttct
      961 cgccgaagga caagatcggc gactggctgg aaatgtacac gaaggtgatg gagctgaatt
     1021 actggggctc caccgtctgc gagaaggcct cctacgatcc cgagaagaag gaatggaccg
     1081 tcgaggtcgt gcgcaacggc cagccggtga cgctgcggcc caagcagctg gtgctggcca
     1141 ccggcatgtc ctccttcccc aacattccga aggatccggg ccatgagacc tttctgggcg
     1201 agatccagca ctcaagccag catccaggcc ccgacgccta tgccggcaag aaggtggtgg
     1261 tgatcggatc caacaactcc gcgcacgaca tctgcgcggc gctttgggaa gtgggcgccg
     1321 acgtcaccat ggtccagcgc tccaccacgc atatcgtgaa gtccgacacg ctgatggata
     1381 tcggcctttc cgccctctac tcggagaagg cggtcaagag cggcatcgac caccacaagg
     1441 ccgatctgat cttcgcctcg gtgccctaca gggttctgcc ggccattcag accccgcttt
     1501 acgagcagat gaagaagaag gatgccgggt tctacaagcg cctcgagaag gcgggcttca
     1561 tgctggactg gggcgaagac ggctcgggcc tcttcatgaa gtatctccgc cgcggctccg
     1621 gctactacat cgacgttggt gcgtccgagc tcgtctgcga cggcaagatc aagctcaaga
     1681 gcggcgtgtc ggtgaaggag atcaggccgc actccatcgt cttctccgac ggcagcgaac
     1741 tggaggccga tctcatcatc tatgccaccg gctattcctc gatgaacggc tgggccgcca
     1801 agctgatcag ccaggaggtg gccgacaagg tcggcaaggt ctggggcctc gggtccggca
     1861 ccaagaagga tcccggcccc tgggagggcg aactccgcaa catgtggaag ccgacgcagc
     1921 aggaggcgct gtggttccat ggcggcaacc tgcaccagtc acgccactac tcgcagttcc
     1981 tgtcgctgca gctcaaggcg cggcaggaag gcattccgac gccggtctac ggactgccgg
     2041 aggttcatca caaggcgtga atgcggaagg gcccctcgcg gggcccttct cgcgttggac
     2101 tggacgtgtg cccttcaggc ggtgcggcga acaggcatca cgcggttgag cgccaacccc
     2161 gcgccgaggc ccgcgccatg caggatgacg gtcccggcga tgaagccaag ggcatagtcg
     2221 agggccgagg cgccgatcgg catttcaacc gtatgggcat agccgtggaa cacggcgaag
     2281 gcgccggcga gcattgtggc caccaccagc gggatgctgc ggcggatgag ggctgcaagg
     2341 ccgagaatgc cgaccgaaag caggatgccg gcttcgacgt atggaaggcc g
//
LOCUS       DBNGVR010000041         5296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1783783, whole genome shotgun sequence.
ACCESSION   DBNGVR010000041 DBNGVR010000000
VERSION     DBNGVR010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1783783"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="AC16A0_00820"
     CDS             <1..301
                     /locus_tag="AC16A0_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3103897.1"
                     /translation="KWWLSPEIQHQFAANGGQSAIKAVYEDPKYVTYRPWNRAWAPQL
                     DWQKDVWHVPQFFELLTQQQDQFDLAITGKQDAKTTLDNIAKFQQNLLTEAGLIQ"
     gene            381..1442
                     /locus_tag="AC16A0_00825"
     CDS             381..1442
                     /locus_tag="AC16A0_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3103898.1"
                     /translation="MNTLAAAQQPPSFFSVAPDNWKLAIVVGLIVSILAAVLLPALAS
                     FWIVGAMMALVAAAFVVNAYRRRYYGGLLVAPAIAVLFVMNIFPLLWSLGLSFFAYQS
                     NQQSIRFIGLNNYIKVLTNDLAAEANWNALINTAMFVVFTVSAQMIVGFLLAMLFAKQ
                     FPLRKYLLILVLTPMMLSVVASGVFFTYYYDPTFGILSYVMGHITGEQFILMDNKVGA
                     VAGIVFADAWMWSPFVMLLVLAGLVSVPKYLYEAAAIDRVSAWRRFWNITFPYIRGLL
                     MLALLFRTIEAFKLADLVILLTKGGNDTMTISYHLIRIANEQNKTSEGAAISYLMLFM
                     VIVLTNLYLYMANRRSQGT"
     gene            1446..2390
                     /locus_tag="AC16A0_00830"
     CDS             1446..2390
                     /locus_tag="AC16A0_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3103899.1"
                     /translation="MSSAIPFLERLGLRKASGVGEAGWGRTLVVAIVSFIYFLPVLFI
                     IFTAIKPQDMALSVPPTLSPTSIFGLIPDAYVFSPTLENFTSVFSRSMTTGGAPEPTG
                     FDRYFFNSIVIATVSVLLALVIGTLAAFGFSRYPLKGNDTYLFIILTTRMLPAIVVII
                     PVILMFRVAGLSGSYIGIILLYTAFNLAFTIWMMKSFFDELSTDVEDAARIDGSSDVK
                     VFFKICLPQVVAGLAATFVFGLILTWNEFLFALLLSGPDTRTVPVAMNQAVSSGGRGT
                     DWTLLAAIETLFLIPVFLVTFFLQDHLLRGVTFGTVKR"
     gene            2392..3480
                     /locus_tag="AC16A0_00835"
     CDS             2392..3480
                     /locus_tag="AC16A0_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3103900.1"
                     /translation="MSTIEIRKVTKRFGDFVAVRDADLAVGAGEVVCLLGPSGCGKTT
                     TLRMIAGLERATSGDVIIGGQRVNDLTPEKRNIAMVFQFYALYPALTVGENIAMPLHY
                     ENLSKSELDARVMKVATILHLTEVLDRMPGQVSEGEKQRIAVARAIVRDPSCFLFDEP
                     LSRLDVELRHSMRGQIKEVLSGLSKATVIVTHDQLEALTMADRIAIMRDGIIEQVGTP
                     HDVFAKPANVFVASFIGTPQMNIVDADLKSFAAGQATVELAGSPLKIAADPAVSGLKP
                     SKVKIGIRPRAFSAVDRASADTIMAQAEQIEPKRAETLIHARTKIGDDIRVVVPREMR
                     VKGGETLHLRPDPRQTHFFGEDGKAVRA"
     gene            3492..3911
                     /locus_tag="AC16A0_00840"
     CDS             3492..3911
                     /locus_tag="AC16A0_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103901.1"
                     /translation="MTPRGALRLEYFIIGLGIFALLLIFQPFSLKLFAIGSGLVVLAG
                     LINNLLPLARPGVPVSSVINVAMIVAMIFCIVLLISIAAAHLYGVFFLKPPDPNTTAG
                     KVQLATKPFYLQPLVWYIAAVAAALAVAITVRVKSAR"
     gene            3913..4857
                     /locus_tag="AC16A0_00845"
     CDS             3913..4857
                     /locus_tag="AC16A0_00845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3103902.1"
                     /translation="MRYDVSVIGLYILDVLGRPVDRIPDRGNVDFIEEIRLTVAGTAG
                     GTVVDTAKLGLRSLAVGAVGDDEKADWVLLTMQKHGIDTSAMQRLSGVPTSATILNVR
                     PNGDRPALHVRGASDHFDVSPSMYDQVFDAPIIHLGGTGLLKKLDGSASVTLLREAKS
                     RGRTVTFDLIAASAETLGIVAPLLPYIDYFMPSIEEAKDMSGQKTAEDCAKFYLDKGA
                     SCCVFTLGGEGAFYAHRDGTRLASRAYDITVVDTTGCGDAFDAGFIAALHHKMDTETA
                     LRFAQASAGLVATGLGSDAGIVSFEDTLRAMKNWKIKS"
     gene            4854..>5296
                     /locus_tag="AC16A0_00850"
     CDS             4854..>5296
                     /locus_tag="AC16A0_00850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3103903.1"
                     /translation="MRFAGRRAVVTGGLSGIGEAVAKRIQAEGGRVAVWDMQGGIKTD
                     ISNHESVQAAAAETVRQLGGIDILVNSAGIAGPTATVVDLKLEDWHRTVAINLNGTFF
                     TNRAVVPHMMAQNYGRIVNIASIAGKEGNPNASAYSASKAAVIGFT"
BASE COUNT          954 a         1649 c         1540 g         1153 t
ORIGIN      
        1 gaagtggtgg ctctcgcctg agatccagca ccagttcgcg gccaatggcg gccagtcggc
       61 catcaaggcg gtctatgagg acccgaagta cgtgacctat cgtccgtgga accgcgcctg
      121 ggccccgcag ctcgactggc agaaggacgt ctggcacgtt ccgcagttct tcgaactgct
      181 cacccagcag caggatcagt tcgaccttgc cattaccggc aagcaggatg ccaagacgac
      241 cttggataac atcgccaagt tccagcagaa cctgctgacc gaggcggggc tgatccagta
      301 acagcgacac gtgaagccga gacgcgcggg aaacgccccg cgcgtctcct ttcttatcta
      361 gatcggatgt tcctcacgac atgaatacgc tcgcagcagc tcagcagcca ccgtcgttct
      421 tcagcgtcgc gccagacaac tggaagcttg ccatcgtcgt gggcttgatc gtctctattc
      481 tggcggccgt cctgcttccg gcgttggcgt ccttctggat cgttggagcc atgatggcgc
      541 ttgttgctgc cgccttcgtg gtcaacgcct atcgccgccg ctactatggt ggccttctgg
      601 tggcgccggc cattgccgtg ctctttgtca tgaacatctt cccgctgctc tggtcgctgg
      661 gtctctcatt cttcgcctac cagtccaacc agcagtcgat acgtttcatc gggctcaaca
      721 actacatcaa ggtgttgacc aatgatctgg ccgcagaagc gaactggaac gcgttgatca
      781 atacggcgat gttcgtggtc tttacggttt cggcgcagat gatcgtcggc tttctgctgg
      841 ccatgctctt tgccaagcag tttccgcttc gcaagtacct cctcattctc gtgctcacac
      901 cgatgatgct ctccgtcgtc gcatcgggcg tcttcttcac atactattac gacccaacct
      961 tcggcatcct cagttacgtg atgggacaca tcaccggcga gcagttcatc ctcatggaca
     1021 ataaggtcgg tgcggtggcc ggcatcgtct tcgccgatgc gtggatgtgg tcacccttcg
     1081 tcatgctgct ggtgctggcc gggctggtat ccgtaccgaa gtatctttac gaggccgctg
     1141 ccatcgaccg cgtctccgcc tggcggcgct tctggaacat caccttcccc tatatccgtg
     1201 gcctgctgat gctggcgctg ctgttccgaa ccatcgaggc cttcaagctt gccgacctcg
     1261 tgatcctgct gaccaagggc ggcaacgaca cgatgaccat ctcctatcac ctgatccgca
     1321 ttgcaaacga gcagaacaag acgagcgagg gtgcagcgat ctcttacctc atgctcttca
     1381 tggtgatcgt gctgaccaat ctctacctct acatggcgaa ccgccggagc caaggaacct
     1441 gatccatgtc atctgccatt ccattccttg aaagactcgg gcttcgcaag gccagcggcg
     1501 tcggcgaagc cggctgggga cggaccctcg tcgttgccat tgtctccttt atctacttcc
     1561 tgccggtgtt gttcatcatc ttcacggcca tcaagccgca ggacatggcc ctgtcggtgc
     1621 cgccgacgct gtctcccacc tcgatcttcg gactgatccc ggatgcctat gtgttttccc
     1681 cgacgctgga gaacttcaca tccgttttct cgcggtcgat gacaaccggg ggggcgccgg
     1741 aacccacggg cttcgaccgc tatttcttca actccatcgt gatcgccacc gtgtccgtgc
     1801 tgctcgccct ggtgatcggc acgctggcgg cattcggttt ctcgcgctat ccgctcaagg
     1861 gcaatgacac gtatctcttc atcatcctga ccacccgcat gctgccggcc atcgtcgtga
     1921 tcattccggt gatcctaatg ttccgcgtcg ccggactgtc ggggtcctat atcggcatca
     1981 tcctgctgta caccgccttc aatctggcct tcaccatctg gatgatgaag agtttcttcg
     2041 acgagctgtc gacggacgtc gaagatgccg cgcgcatcga cgggtcctcc gatgtgaagg
     2101 tcttcttcaa gatctgcctg ccacaggttg ttgcgggtct ggctgcgacc tttgtcttcg
     2161 gcctcatcct cacctggaac gagttcctct tcgcgctgct cctctccggg ccggatacgc
     2221 gcaccgtccc cgtcgccatg aaccaggccg tatcttccgg cgggcgcggg acggactgga
     2281 cgctgctggc agcgatcgag acgctcttcc tgatccctgt cttcctcgtc accttcttcc
     2341 tgcaggatca tcttctgcgg ggtgtcacct tcggaacggt aaagcgctga catgtcgacg
     2401 attgaaatca gaaaggtcac caagcgcttc ggcgatttcg tcgcggtccg cgatgcggac
     2461 ctggccgtgg gtgcgggcga ggtggtttgc ctgctcggtc cgtctggctg cggcaagacc
     2521 acgacgctgc gcatgatcgc gggcctcgaa cgcgccacct ccggcgatgt gatcatcggc
     2581 gggcagcgcg tcaacgacct gacgccggag aagcggaaca ttgccatggt gttccagttt
     2641 tatgcgctct atccggcgct gacggtgggc gagaatatcg cgatgccgct ccactacgag
     2701 aatctgtcga agtccgaact cgatgcgcgc gtgatgaagg tggccacgat ccttcatctg
     2761 accgaggtac ttgaccggat gcccggccag gtctcggaag gcgagaagca gcgcatcgcg
     2821 gtggcgcgcg ccatcgtgcg tgatcccagc tgctttctct tcgacgaacc gctctcgcga
     2881 ctcgacgtcg aattgcggca ctcgatgcgc gggcagatca aggaggttct ctcgggcctt
     2941 tcgaaggcaa cggttatcgt gactcatgac cagcttgaag ccctgacaat ggccgatcgc
     3001 atcgccatca tgcgcgatgg catcatcgag caggtgggga cgccgcacga cgtctttgcc
     3061 aaaccggcga atgtcttcgt cgccagcttt atcggcacgc ctcagatgaa tatcgtcgat
     3121 gccgatctca agtcattcgc tgccggacag gccaccgttg aactggccgg gagtccgctc
     3181 aagatcgcgg ccgatccggc agtgtccggt ctcaaacctt cgaaggtcaa gatcggcatc
     3241 cgtccccggg ccttctcggc ggtggacagg gcttcggccg acacgatcat ggcccaggcc
     3301 gagcagatcg aaccgaagcg ggcggaaaca ctgatccatg ctcgcaccaa gatcggcgat
     3361 gacatccgtg tcgtcgtccc gcgggagatg cgggtaaagg gcggtgaaac tttgcatctt
     3421 cggcccgatc ccaggcagac tcacttcttc ggggaagacg gaaaggcggt gcgcgcatga
     3481 gggatgcggg catgacccca cgaggcgccc tgcgcctaga gtatttcatc attggactgg
     3541 gtatctttgc ccttctcctg atctttcaac ccttcagcct caagctcttc gccatcggca
     3601 gcggactggt tgttcttgcc ggactcatca acaacctgct gcccttggcc agacccggcg
     3661 tgcccgtcag ttccgtcatc aatgtcgcca tgattgtggc gatgatcttc tgtatcgtct
     3721 tgctgatctc catcgcggcg gcgcatctct acggggtgtt cttcctcaag ccgcccgatc
     3781 ccaacacgac agccggcaag gtgcagctgg ccaccaagcc gttctacctt cagcctctcg
     3841 tatggtacat cgcagcagta gcggcggcgc tcgccgttgc catcactgtg cgagtcaaga
     3901 gtgccagata agatgcgcta tgacgtttcg gtcataggtc tttacatcct cgatgtgctg
     3961 ggtcggccag tggaccgcat tcccgaccgg gggaatgtcg atttcatcga ggagatccgt
     4021 ttgacggttg ccgggacagc aggcggaacc gttgtcgaca ccgcaaagct gggcctcagg
     4081 agccttgccg tgggcgccgt gggcgatgac gagaaggccg actgggttct cctcacgatg
     4141 cagaagcatg gcatcgacac ctctgccatg cagcggctga gtggtgttcc cacttcggca
     4201 accatcctca acgtgcggcc caatggtgat cgccccgcgc tccatgtcag gggtgcctcc
     4261 gaccattttg acgtgtcgcc gtcgatgtac gatcaggtct tcgatgcgcc gatcattcat
     4321 cttggcggga cgggcttgct gaagaaactc gatgggtccg cgagtgtcac ccttctgagg
     4381 gaagccaagt cgcggggccg gactgtcacc ttcgacctga tcgccgccag cgcagagaca
     4441 ttgggaattg tggcgccgct cttgccctac attgactatt tcatgccttc cattgaagag
     4501 gcaaaggaca tgtccgggca gaagacggcc gaggattgcg cgaagttcta cctcgacaag
     4561 ggagcaagct gctgtgtctt cacgcttggc ggggagggcg ctttctacgc ccacagggac
     4621 ggcacgcgcc tggcatcccg cgcctacgac atcacggtgg tcgatacgac tggttgcggc
     4681 gacgccttcg atgccggttt cattgctgcg ctgcaccaca agatggacac cgaaacggcc
     4741 ctgcgtttcg cgcaagcctc ggcgggcctt gtggctactg gcctcggctc ggatgcgggg
     4801 atagtgtcct ttgaagacac gctcagggca atgaagaact ggaagatcaa gtcatgagat
     4861 ttgcaggcag gcgggctgtt gtcaccggcg gattgtcagg gattggcgag gctgttgcga
     4921 agcgcattca ggccgagggc ggcagggttg cggtctggga catgcagggc ggcatcaaga
     4981 ccgacatctc gaaccacgag tcggttcagg ccgctgctgc cgagacggtg aggcagctcg
     5041 gcgggatcga catcctcgtc aacagtgccg gtatcgccgg accgaccgcc accgtggtcg
     5101 atctcaagct tgaggattgg caccgcaccg tcgctatcaa cctcaacggt acgttcttca
     5161 cgaaccgcgc cgtggtgccg cacatgatgg cccagaacta tggccgcatc gtcaacatcg
     5221 cctcgattgc cggcaaggag ggtaatccaa acgcctcggc ctattcagcg tccaaggcgg
     5281 cggtcatcgg cttcac
//
LOCUS       DBNGVR010000042         4974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1980244, whole genome shotgun sequence.
ACCESSION   DBNGVR010000042 DBNGVR010000000
VERSION     DBNGVR010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1980244"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1119)
                     /locus_tag="AC16A0_00855"
     CDS             complement(106..1119)
                     /locus_tag="AC16A0_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3103904.1"
                     /translation="MFRFLLKRLLLTLPTFFALMFVTFIMIRLVPGDPIEVRRGERGI
                     SPERLAELRHEMGLDQPIWKQFFDYVWGILHGDFGTSIISKTPILHEFFTLFPATLEL
                     SICAMIFAVGLGVPAGVIAASRRGGIYDQGLMFTALAGYSMPIFWWGLILILVMSNTL
                     GLTPVSGRVDLIKYYYEPVTGFMLIDSLLSGQKGAFWDAVHHLILPTIVLGTVPLAVI
                     ARMTRSSMLEVLEEDYVRTARAKGLSSSRVIGVHALRNALIPVVTVFGLQVGGLMAGA
                     VLTETIFSWPGVGKWLVESISRRDYPALQGGILLISTIVILINLCIDLLYGLINPRIR
                     HAK"
     gene            complement(1183..2784)
                     /locus_tag="AC16A0_00860"
                     /pseudo
     CDS             complement(1183..2784)
                     /locus_tag="AC16A0_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965062.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2943..3743
                     /locus_tag="AC16A0_00865"
     CDS             2943..3743
                     /locus_tag="AC16A0_00865"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3103905.1"
                     /translation="MAANPPPDTIESFRSDGACVLRGVFGAWVRTLQAGIARNLAAPS
                     ADVKIYQGKDGAGRFVGDYCNWDRIADYRDFIFNSDAAAIGKSLMGSNTVRLFHEHVL
                     VKEPGADVPTPWHQDQPYYCVDGTDTVSLWIPLDSVPRERTLEFVAGSHRWGRYFRPE
                     RFNKQPLNENDGLEPVPDITGNRDQYRILGWALEPGDAVAFNYMTLHGAPANTSKVDQ
                     RRAFSLRLIGDNVRFARREGVVTSPPFRGVRLAHGAVMDAPEFPLLTA"
     gene            complement(3756..4331)
                     /locus_tag="AC16A0_00870"
     CDS             complement(3756..4331)
                     /locus_tag="AC16A0_00870"
                     /EC_number="1.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF016180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:1901053 - obsolete sarcosine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma"
                     /protein_id="HIR3103906.1"
                     /translation="MKAKPAVEIAFPRVSPLADVALQGRFGADRGAPGVEFRVRHPLS
                     ILTVIARKGKSNAVADALAALKGATVQWAGPDQYFVLAEGRGEGALYREARAMLDGLA
                     SVSDQSHGRVVIRVAGMKARNVLAKGTPVDLHPDAFPVGTSAMTQMAHVGVHLTRVAE
                     DAYDLSVFRGFAESFWEWITEQAEEFGYQVK"
     gene            complement(4339..>4974)
                     /locus_tag="AC16A0_00875"
     CDS             complement(4339..>4974)
                     /locus_tag="AC16A0_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR3103907.1"
                     /translation="VPYMAAQEITVLGGVPARLFRISFSGEHAYELAVPADYGNMVAR
                     ALMQAGAEFGITPYGVEALSIMRVEKGHVAGGELNGTTTAGDLGLGKMMSAKKDYIGR
                     MMAQREGLLDPARQCVVGIRPVDKADRIRAGSHVLKREDRPSMANDQGYLTSVAWSPM
                     LDMWIALALVSNGRARHGETVRIWDGLRGTETLGEICDPMHFDRENKRLHA"
BASE COUNT          899 a         1569 c         1605 g          901 t
ORIGIN      
        1 atgctgatga tggcgaggcc gatcaccgcg ccgcggttct cgcggaaggc ccgccagaag
       61 gccgtgaaag gtccgggcgg cgcggtcggt gccttgagtg cggcatcact tggcatggcg
      121 gatcctggga ttgatgaggc cataaagcag gtcgatgcag agattgatga ggatcacgat
      181 ggtcgagatc agaaggatgc cgccctgcag ggccggatag tcgcggcggc taatcgactc
      241 gaccagccac ttcccgacgc cgggccagga gaagatggtt tcggtcagga ccgctccggc
      301 catgagaccg cccacctgca ggccgaagac cgtcaccacc ggaatcagcg cgttgcgcag
      361 ggcatgcacg ccgatgacgc gcgatgacga gagacccttg gcgcgggcgg tgcggacgta
      421 gtcttcttcg aggacttcca gcatggacga gcgcgtcatg cgggcgatga ccgccagcgg
      481 cacggtgccc agcacgatgg tgggcaggat caggtgatgc accgcatccc agaacgcgcc
      541 cttctgtccg gacagcaggc tgtcgatcag catgaagccg gtgaccggtt catagtagta
      601 cttgatcagg tcgacgcggc ccgacaccgg cgtgaggccc agcgtgttcg acatgacgag
      661 aatgagtatc aggccccacc agaagatggg catggagtag ccggccagcg ccgtgaacat
      721 caggccctgg tcatagatgc cgccacgccg cgaggcggcg atcacgccgg ccggaacccc
      781 caggcccacg gcgaagatca tggcgcagat gctgagttcc agcgtggccg gaaacagcgt
      841 gaagaactcg tgcaggatgg gcgtcttgga aatgatcgag gtaccgaagt cgccgtgcag
      901 gatgccccag acatagtcga agaactgttt ccagatcggc tggtcgaggc ccatctcgtg
      961 gcgcagttcc gccagcctct cgggcgagat tccgcgctcg ccgcgccgca cttcgatcgg
     1021 atcgcccgga accaggcgga tcatgatgaa ggtcacgaac atgagtgcga aaaacgtcgg
     1081 cagcgtgagg agcagacgct tcaggagaaa tcggaacatc aggatgtttc gtccgtgagg
     1141 aaggaagcgg gcccgcaggc ccgcttccct gactgccggt gattactcgg cgatgtcgac
     1201 gttctggaac tcatgcgagc cgaaggggct gatcttgtag ccctggactt ccttgcgcac
     1261 cggttcgaaa acggtggagt gggccacgtt gagccacggc acttcggccg ccacgatctg
     1321 ctgcgcttcg acatagagct tggtgcgctc gtccttgtcg gagatctcgg cggcctagtt
     1381 cacgacttcc tggaagcggc cattgcacca cttcggaatg ttgttgccgt tcggcttgcc
     1441 ctcggcgttg cagcccagca gcacggcgag gaagttatcc gggtcgccgt tgtcgccggt
     1501 ccagccaagc aggcccatct ggtgctcgcc cgcctgcaga cgcttgcggt actcgcccca
     1561 ctcgtaggac accaacgttg cgtcgatacc gaccttggcg aggtccgcct gcatcatttc
     1621 cgccatgcgc ttggcattcg gattgtacgg gcgctgcacc ggcatccacc agatgtcggt
     1681 cttgaggttc tccacgcctg ccgccttcag catttccttg gccttgtccg ggtcaaaggg
     1741 aatgtcgacg gtggcttcgt cataggacca catggtcggc gggatcgggt tcttggcctg
     1801 ctggccggct ccctggtaga cttccttgag gatcgcaccg cggtcgatgg ccatggcgat
     1861 cgccttgcgg acttcaggct tgtcgaaggg cggcttctgg gcgttcatcg cgagatagcc
     1921 gatgttgagg ccggcttgcg acatgaggtt gatgtcggca tccttgctca tggcctcgag
     1981 atcagccggg ttcggcgcga tcatcacctg gcactcattg gccttgagct tggcatagcg
     2041 cgcggtcggg tccggcgtga tggcatagac cagatcatcg atcttcggct tgccggccca
     2101 gccgtcgaag gccttgaagc ggatgactgc gtccttctgg taggccacga aggagaagga
     2161 gccggtgccg accgggatct ggtcgaactc ttcgggcgcg cccttcttgg tgaggaagtc
     2221 ggcgtattcg gccgagtgaa tggtggcgaa atccatggcc aggttggcca ggatcggcgc
     2281 gttgggcttc ttcagcttga tcacgacggt gagatcgtcg ccgcccttct cgatcgactc
     2341 gagcagatcg ggcatgctca tgtcgttgaa gtagtcatag gagccgcccg acacctttgc
     2401 gtaaggatgt tccgggttcc acatgcggcc gaaggaccag atcacgtcct cggcggtgag
     2461 atcgcgggtc ggggtgaagc cgttcacacc ggagtggaac ttcacgccgg gacgcagctt
     2521 gaaggtgagc gtcttgccat cttccgagat ggtccagctc tccgcgagtg caggaaccac
     2581 actggtgccg ccgggttcga actggacgag acggttgtag accggacggg ccgcatcgaa
     2641 actggtaccc gtggtgttca tcgaagggct aaagttctct ggcgaacctt ccgagcaata
     2701 gaccagcgat ttcgcctggg ccgcgccgac cgccagcatc gaggatgcca aaagcgccgc
     2761 gacgagcgtt ttgttgaatc tcatgtttca ctccctgagt ttacgtacgc ccccctgggc
     2821 gtaaacccac ctaagcacgt tttgtcaggc caatcaaaat ctcgcatgcg caccccgtga
     2881 aggaagcaat ttacctcgcg gccctctgct ggcagactcc gcggaaacaa ggatagaccg
     2941 caatggccgc aaacccccct ccggatacca tcgaatcctt ccgttccgac ggggcctgcg
     3001 tcctgcgcgg cgtcttcggg gcctgggttc ggacattgca ggcaggcatc gcccgcaacc
     3061 ttgccgcgcc gtcggccgac gtgaagatct accaaggcaa ggatggcgcc ggacgcttcg
     3121 tcggcgacta ttgcaactgg gaccgcattg ccgactacag ggacttcatc ttcaactcgg
     3181 atgccgccgc gatcggcaag tcgctcatgg gcagcaacac cgtgcggctc ttccacgagc
     3241 atgtgctggt gaaagaaccg ggcgcggatg tgccgacacc ctggcaccag gatcagccct
     3301 attactgcgt ggacgggacc gacaccgtct cgctgtggat tccgctcgac agcgtaccgc
     3361 gcgaaaggac cctcgaattc gtggccggct cccaccgctg gggcaggtat ttccgccccg
     3421 agcgcttcaa caagcagccg ctcaacgaga atgacgggct cgagccggtg cccgacatca
     3481 ctggtaaccg cgaccagtac cgcatcctag gctgggcgct ggagccaggc gacgcggtcg
     3541 ccttcaacta catgacactg cacggcgcgc ccgccaatac ctcgaaggtg gaccagcgcc
     3601 gcgccttctc gctccgcctc atcggcgaca atgtccgttt tgcacgccgc gagggcgtgg
     3661 tcacctcgcc gccctttcgc ggcgtgaggc tggcgcatgg cgccgtgatg gacgcgcccg
     3721 agtttccact gctgacggcc tgagaagcaa gcgggctatt tcacctgata gccgaattcc
     3781 tcggcctgtt cggtgatcca ttcccagaag ctttccgcaa agccgcggaa gaccgagaga
     3841 tcgtaggcat cttcggccac gcgggtcagg tggacaccca catgcgccat ctgggtcatc
     3901 gccgacgtgc ccactggaaa ggcatcgggg tggagatcga ccggagtccc cttggcaaga
     3961 acattgcgag ccttcatgcc cgccacgcgg atgaccacgc ggccatggct ttgatccgac
     4021 accgatgcga ggccgtcgag catcgccctt gcctcgcggt agagcgcgcc ttcgccacgg
     4081 ccttcggcca ggacgaaata ctggtctggc ccggcccatt gcaccgtggc gcccttgagc
     4141 gcggcgagcg cgtcggccac ggcattggat ttccccttgc gcgcaatgac cgtgagaatg
     4201 ctgagggggt gacgaacgcg gaactctacg cccggcgcac cgcggtcggc gccgaagcgc
     4261 ccctgcagcg ccacgtcggc caggggcgag acgcgcggga aggcgatctc cacggcaggt
     4321 ttggctttca agggcgttct aggcatggag tcgcttgttc tccctgtcga aatgcatggg
     4381 atcgcagatc tcgcccagcg tctcggtgcc gcgcagccca tcccagatgc gcacggtctc
     4441 gccgtggcgg gcgcggccgt tgctcaccag cgccagcgca atccacatgt cgagcatggg
     4501 cgaccaggcg acggaggtga gatagccctg gtcgttcgcc atcgagggcc ggtcctcgcg
     4561 cttcaggaca tgcgagccgg cgcggatgcg gtcggccttg tcgaccggcc ggatgcccac
     4621 cacgcattgc cgcgccggat cgagaaggcc ctcgcgctgc gccatcatgc ggccgatata
     4681 gtccttcttg gccgacatca tcttgccgag gccgaggtcg ccggccgtcg tggtgccgtt
     4741 gagctcgccg cccgccacgt ggcccttctc gacgcgcatg atcgacagcg cctccacgcc
     4801 gtaaggcgtg atgccgaatt ccgcgcccgc ctgcatcagg gcgcgggcaa ccatgttgcc
     4861 gtagtcggcc ggcaccgcca gctcataggc atgttcgccc gaaaacgaga tgcggaagag
     4921 ccgtgccgga acgccgccca gcacggtgat ctcctgtgct gccatgtagg gcac
//
LOCUS       DBNGVR010000043         3940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_605254, whole genome shotgun sequence.
ACCESSION   DBNGVR010000043 DBNGVR010000000
VERSION     DBNGVR010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_605254"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1158
                     /locus_tag="AC16A0_00880"
     CDS             172..1158
                     /locus_tag="AC16A0_00880"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HIR3103908.1"
                     /translation="MRPAPIIDALQYCNWSAEIFRQMREGGVTAVHVTICYHETFRET
                     VANIAAWNRRFEEHRDTLLHARSGADVRQAQAEGRTAIIFGLQNCSPIEDDIGLVEIC
                     HQLGIRFMQLTYNNQSLLASGCYESEDSGITRMGREVIREMNRVGLVIDMSHSGERST
                     LEAIDISARPIAVTHANPSFWHPGRRNKSNAVLKALTARHGMLGFSLYPHHLKDGSHC
                     RMEDFCAMVARTAEMVGADHLGIGSDLCQNQPDSVVTWMRSGRWTRTPDFGEGSAAAA
                     GFPPQPPWFRDNRDFHKIAEGLRAVGFSGTEVDGIMGVNWLRFFDDSFVPAH"
     gene            1133..1342
                     /locus_tag="AC16A0_00885"
     CDS             1133..1342
                     /locus_tag="AC16A0_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103909.1"
                     /translation="MTASSLRIETPAVALRPPSQDMRLLRMGSCFATRLSFMRVMVRE
                     LKAGYLMRTTAVHGIGKFGTADTAS"
     gene            1406..2155
                     /locus_tag="AC16A0_00890"
     CDS             1406..2155
                     /locus_tag="AC16A0_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3103910.1"
                     /translation="MSDMFSLKGRVALVTGASRGLGLAMARALVDAGAHVVVNARDAT
                     ALAEAADQIGAEAMAFDVTDAKVARSALEDVAARHGKLDILVNNAGIQHRRAITDWED
                     EDFDRVIAVNLTSCFRLARDAVRIMLPHRHGRIINTGSVAAILGRPTIHAYAAAKAGL
                     HGLTRSMAAELGRQGITVNAIAPGYFATELNSALLADKAFTAWVEGRTPAGRWAKPEE
                     LGGAAVFLASDAASYVNGHVLAVDGGLSVSL"
     gene            complement(2159..2419)
                     /locus_tag="AC16A0_00895"
     CDS             complement(2159..2419)
                     /locus_tag="AC16A0_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103911.1"
                     /translation="MKTLVALALAAFIASPAVAANVTNADGDAHVLVVTEDGEQSEVG
                     VNPGQTVDICPNGCFVNMPNGDREALTGSETIEISGGKAVFK"
     gene            complement(2503..>3940)
                     /locus_tag="AC16A0_00900"
     CDS             complement(2503..>3940)
                     /locus_tag="AC16A0_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3103912.1"
                     /translation="LLSLAIAVVQVADYFFGRVNSIFASPLSLTVAKSFVSSITISLL
                     LALVAVAKPFTDEATGARREWPGWIRTPLLLIALFIIVAALSGYVGLARFTSAQVVVT
                     GAILATMFIGVQSGHILAAEGAFPRSALGQKVKTWFSISDTALDQLGLLLSFLVYFVV
                     LAIGLPLILLQWGFNQIDIRTWLYKAFTDITIGTVSISLVGILFGILLFALGFLFTRQ
                     FQFWLDRTVMARSRVDPGVRNSIRTVVGYLGVAIAGMIGLSAAGFDLSNLAIVAGALS
                     LGIGFGLQNVVNNFVSGLILLAERPFKVGDWIETGKASGLVKKISVRATEIETFQHQT
                     VTLPNSELINSAVGNWTHRNNWGRVDIPITAPVGRHPREVYDILIEIGSKDPAVLKSP
                     APTAIFKGINGAVMEFELRVHIPEVFDSSVVATRIRFEIFDAFERIAMDTAPVPRANL
                     TLSLPDLEALAQAIDKVKAKPRRRRPER"
BASE COUNT          770 a         1207 c         1286 g          677 t
ORIGIN      
        1 ggccgcatcg agttacgggg ggctgggggg ctgagatatc cggtcctcaa caccagccgg
       61 gccatagagg caacatcatc aagttcgccg atgaaggggg cgacagcttg atgcaaatgc
      121 ggcggaaagg tgaattcttg ccctgccccg gcagacgcct atgatgagcc catgagaccc
      181 gcccccatca ttgatgcact gcagtattgc aactggtcag cggaaatctt tcgccagatg
      241 cgcgaggggg gcgtcaccgc ggtgcacgtg accatttgct atcatgagac ttttcgcgag
      301 accgttgcca acatcgcggc gtggaaccgg cgcttcgagg agcaccgcga cacgctcctg
      361 cacgcccgct ccggcgccga cgtgcggcag gcccaggcgg aagggcggac cgcgatcatc
      421 ttcgggttgc agaactgctc tcccatcgag gacgacatcg ggctggtgga aatctgccac
      481 caactcggca tccgtttcat gcagctcacc tacaacaacc agtcgctgct ggcctccgga
      541 tgttacgaat cggaggatag cggcatcacc cgcatggggc gcgaggtgat ccgggaaatg
      601 aaccgggtgg ggctggtgat cgacatgagc cattccggag aacgctcgac gctggaagca
      661 atcgacatct cggcgcggcc cattgctgtg acccacgcaa atccgtcctt ttggcatccg
      721 ggacgccgca acaagtccaa tgccgttttg aaagcgctca cggcgcgcca cggcatgctg
      781 ggcttttcgc tctacccgca ccatctgaag gacggctccc actgccgcat ggaggatttc
      841 tgcgccatgg tggcgcggac cgccgagatg gttggcgccg accatctggg catcggcagc
      901 gatctctgcc agaaccagcc cgacagcgtg gtgacctgga tgcgcagcgg gcgctggacc
      961 aggacaccgg acttcggcga gggctccgct gctgcagcag gctttccgcc gcagccgcca
     1021 tggttccgcg acaaccgcga tttccacaag atcgcggagg gcctgcgggc ggtcggcttc
     1081 agcggcaccg aggtggacgg catcatgggc gtgaactggc tgaggttctt cgatgacagc
     1141 ttcgtccctg cgcattgaga cgccggccgt tgccctgcga ccgccgtcgc aggacatgcg
     1201 gctgttgcgc atggggagct gctttgccac ccgtctgagc ttcatgcggg tgatggtgcg
     1261 ggaactgaag gccggctacc tgatgcggac cacggccgtc catggcatcg gcaaattcgg
     1321 cacagccgac accgcatcgt gagcggtctt gcgtgcaacg cagcctgtga tgcaggatcg
     1381 atcatcggga gccaggggac aggacatgag tgatatgttt tcgttgaagg gacgcgtggc
     1441 actggtcact ggagcctcgc gcggccttgg ccttgccatg gccagagcac tggtcgatgc
     1501 cggcgcgcat gtcgtcgtca acgcccgcga tgcgactgct ctggcagagg cggcagatca
     1561 gatcggcgcg gaggccatgg ccttcgacgt gaccgatgcc aaggtcgcgc ggtcggctct
     1621 cgaggacgtt gcggcacggc acggcaagct ggacatcctg gtgaataatg ccggcattca
     1681 gcatcgccgc gccatcaccg actgggagga tgaagacttc gaccgcgtga tcgcggtcaa
     1741 tctcacctcg tgtttccgcc tggcccgcga tgccgtgcgg atcatgctgc cccacaggca
     1801 cggccgcatc atcaatacgg gatccgtggc ggcaattctc ggccggccca ccatccatgc
     1861 ctatgccgca gccaaggcgg gactgcatgg tctgacgcgc tccatggcag cggaactggg
     1921 acgccagggc attaccgtca acgccattgc gcccggctac ttcgcaacgg aactcaacag
     1981 cgcattgctc gcggacaagg ccttcaccgc ctgggtggag ggccgtacac cggcgggacg
     2041 ctgggcgaaa cccgaagaac tcggcggggc cgccgtcttc ctggcgtcgg acgcagcgag
     2101 ctatgtcaac ggccacgtcc tggccgtcga cggcggtctg tcggtttcct tgtagcggtt
     2161 acttgaatac ggccttgccg ccgctgatct cgatggtctc ggaacctgtg agagcctcgc
     2221 gatcgccatt gggcatgttg acgaaacagc cattgggaca gatgtcgacc gtctgaccgg
     2281 ggttcacccc gacctcgctc tgctcgccgt cctccgtcac gaccaggaca tgggcgtcgc
     2341 cgtccgcgtt ggtcacgttc gccgccacag cgggcgatgc gatgaaggcg gcaagggcaa
     2401 gggcaaccag ggttttcatt tcaactctac tctcggtttc ggggctccta ccccatgata
     2461 tcaaactaga ggcagccgga tgaatggcac ctgaacgcag gttcacctct ccggcctgcg
     2521 ccgtctcggc ttggccttaa ccttgtctat ggcctgcgcc aaggcctcga ggtctggcag
     2581 gctcagcgtg aggttcgccc gcggaacggg cgcggtatcc atcgcgatcc tctcgaaggc
     2641 atcgaagatc tcgaagcgga tgcgggtggc aacaacgctg gaatcgaaga cttccggaat
     2701 atggacgcgc aactcgaact ccatcacggc cccgtttatg cccttgaaga tggcggtcgg
     2761 cgccggagat ttcaagacgg ccgggtcttt cgagccaatt tcaataagaa tatcatagac
     2821 ttctctgggg tgccggccaa caggcgcggt gatcgggatg tcgacgcgcc cccagttgtt
     2881 gcgatgggtc cagttgccca ccgccgagtt gatcagctcg gaattcggca gagtcacggt
     2941 ctggtgctgg aacgtctcga tctcggtggc gcgcaccgag atcttcttga ccaggcccga
     3001 ggcctttccc gtttcgatcc agtcgcctac cttgaagggc cgttccgcca gcaggatgag
     3061 gccggagacg aaattgttca ccacgttctg aaggccgaag ccgataccga gagagagggc
     3121 gccggcgacg atcgcgaggt tcgagagatc gaagcccgca gctgacaggc cgatcatgcc
     3181 cgctatcgcc acgccgaggt agcccaccac ggtgcggatc gagttgcgta cgccgggatc
     3241 gacgcgggac cgcgccatga cggtgcggtc cagccagaac tggaactgcc gcgtgaacag
     3301 gaagcccagg gcaaagagaa ggatgccgaa caggatgccg accagcgaga tcgacaccgt
     3361 cccgatggtg atgtcggtga aggccttgta gagccaggtg cgaatgtcga tctggttgaa
     3421 gccccactgc agaagaatga gcggcaggcc gatggcgagg accacgaaat agaccaggaa
     3481 ggacagcagc agcccaagct gatccagcgc cgtgtcggag atcgagaacc aggtcttcac
     3541 cttctggccc agggccgagc gcgggaaggc accttcggcc gcaaggatgt ggccgctctg
     3601 cacgccaatg aacatggttg caaggatggc gccggtcacc accacctgag cggacgtgaa
     3661 gcgtgcgagg ccgacataac cggacagcgc cgccacgatg atgaagagcg cgatgagcag
     3721 cagcggcgtc ctgatccagc cgggccactc gcggcgcgcg ccggttgcct cgtcggtaaa
     3781 gggcttggcg acggctacaa gcgccagaag caggctgatg gtgatggacg acacgaagct
     3841 cttggccacg gtcagcgaca ggggcgaggc aaagatggag ttcacgcggc cgaagaaata
     3901 gtcggcgact tgcacaaccg cgattgcaag cgacaggagg
//
LOCUS       DBNGVR010000044         2612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4338037, whole genome shotgun sequence.
ACCESSION   DBNGVR010000044 DBNGVR010000000
VERSION     DBNGVR010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4338037"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="AC16A0_00905"
     CDS             <1..1197
                     /locus_tag="AC16A0_00905"
                     /inference="COORDINATES: protein motif:HMM:NF052686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-apionate lactonase family protein"
                     /protein_id="HIR3103913.1"
                     /translation="ARGKIPAIGVGIPMAEAKAALDRAKLVAAAGPSHLVCQIDGRKD
                     GQAEAAAAFAALKSRTGKPVTLEIVLPARESADKEVAAIAAAVTQGGLKPDAVVVTQM
                     HDLKSFQPNTPRPWGPTYEEMAEACRKHFPGVTLGGGMLSYFTELNRKPAPRGVFDFV
                     THTVCPIVHAADDISVMETLESLPSIFASTRNMMGRTPYHLGPSGIPCRDNPYGAAVA
                     GNSENGRVCLADMDPRQRGLFAAAWSLGLAAAAARGGLDAIALGAATGPQGVIYRKAS
                     YAQPWFDGGNAAVYPAYHVLAGLAAMSGAKRLDVASSNSSAVEAVASQTRDGATLWLA
                     NLTGDTQMARLQGFRGPAEIHTLDAASFAAAARKADHFGKPGKTVKSLGRIELGPYAV
                     ARIRWA"
     gene            1201..2460
                     /gene="otnK"
                     /locus_tag="AC16A0_00910"
     CDS             1201..2460
                     /gene="otnK"
                     /locus_tag="AC16A0_00910"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HIR3103914.1"
                     /translation="MLLGCIADDFTGATDLANTLTRRGMHTVQTIGLPRGDAPAADAI
                     VVALKSRTIPAADAVAQSIAACRWLKANGARQIFFKYCSTFDSTDAGNIGPVADALLD
                     ELGAGFTIACPAFPENKRTIYLGHLFVGNELLSDSSMRNHPLTPMRDANLVRVLGRQT
                     PHKVGLVSYAEVKRGSLAIADRFAALQAEGVRHAIVDAVADVDLENIGAACMSLPLVT
                     GGSGVALGLPANFIRTGRLKQRDGARPLAKAGGLAAVLAGSCSAATLAQVAAMRAKAP
                     AFALDPMALHADRGAAVAEALAWAASRLEKGPILIHSTAEPEAVSRAQAALGREAAGA
                     LVEESIAAIARELVARGVRRLVVAGGETSGAVVSALGVTALEIGPQIAPGVPATLSYG
                     EPRLALALKSGNFGGPEFFTEALEALQ"
     gene            2457..>2612
                     /locus_tag="AC16A0_00915"
     CDS             2457..>2612
                     /locus_tag="AC16A0_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3103915.1"
                     /translation="MNETQAREFIAEIGRSMFERRLTFGSSGNISVRIDGGWLMTPTG
                     SSLGRLDP"
BASE COUNT          408 a          924 c          859 g          421 t
ORIGIN      
        1 gccaggggca agatcccggc catcggtgtc ggcattccga tggcagaggc gaaggccgct
       61 cttgaccgcg ccaaactcgt ggctgccgcc ggtccgtcgc acctcgtctg ccagatcgac
      121 gggcgcaagg atggccaggc ggaagccgca gcggccttcg ccgcgttgaa atcccgcacc
      181 ggaaagccgg tcacgctgga gatcgtgctg cccgcaagag agtcagccga caaggaagtc
      241 gccgccattg cagccgcggt gacccagggc gggctcaagc ccgacgccgt ggtggtcacg
      301 cagatgcatg acctcaagtc cttccagccc aacacgccgc gcccgtgggg accgacttac
      361 gaggagatgg cggaagcctg ccgcaagcac ttccccggcg tgacgcttgg cggcggcatg
      421 ctctcctact tcacggaact caaccgcaag cccgcgccac gcggagtctt cgacttcgtc
      481 acccacaccg tctgtcccat cgtgcatgcc gccgacgaca tctcggtgat ggagacgctc
      541 gagtcgctcc cctcgatctt cgcctcgacg cgcaacatga tgggcaggac gccctatcac
      601 ctgggtcctt ccggcattcc ctgccgcgac aatccctatg gcgcggcagt agccggcaat
      661 tccgaaaacg ggcgggtctg cctggccgat atggacccgc gccagcgcgg cctctttgcc
      721 gcggcctgga gtctggggct cgcggcagcg gcggcgcgcg gcggtctcga tgccatcgct
      781 ctcggcgcgg ccaccgggcc gcagggcgtc atttaccgga aggcgtccta cgcgcagccc
      841 tggttcgacg gcggcaatgc cgcggtctat ccggcctatc atgtgctggc gggccttgcg
      901 gccatgagtg gcgccaaacg tctcgatgtg gcatccagca atagctcggc cgtcgaagcc
      961 gtcgcgagcc agacacgtga cggcgccacg ctgtggctcg ccaatctcac cggcgacacg
     1021 cagatggcac ggctgcaggg cttcaggggc ccggccgaga tccacacgct cgatgccgcc
     1081 agcttcgctg cggcggccag aaaggccgac cacttcggca agccaggcaa gacggtcaag
     1141 tcgctcggca ggatcgaact cggtccctac gccgtcgcgc gaatccgctg ggcctgaacg
     1201 gtgctgctgg gctgcatcgc cgacgacttc accggcgcca cggatctcgc caacacgttg
     1261 acgcggcgtg gcatgcacac ggtgcagacc atcggcctgc cgcgcggtga tgcgcctgcg
     1321 gccgacgcca tcgtggtggc gctgaagtcg cgcaccatcc cggccgccga cgcggtggca
     1381 cagtcgatcg cggcctgccg ctggctcaag gcgaacgggg cgagacagat cttcttcaaa
     1441 tactgctcga ccttcgattc caccgacgcc ggcaatatcg gccccgtcgc cgatgcgctc
     1501 ctcgacgagc tgggcgctgg cttcaccatc gcctgccccg ccttccccga gaacaagcgg
     1561 accatctatc tgggccatct cttcgttggc aacgaattgc tctcggactc cagcatgcgc
     1621 aaccatccgc tgacaccgat gcgtgacgcc aatctggtgc gcgtcctcgg ccgtcagacg
     1681 ccgcacaagg tgggccttgt ctcctatgcc gaggtgaagc gcggttcgct ggcgatcgcc
     1741 gaccgcttcg cggccctgca ggcggagggc gtacgccacg ccatcgtcga tgccgtggcg
     1801 gatgttgacc tcgagaacat cggcgcggcc tgcatgtcgc tgccgctggt gacgggaggc
     1861 tccggggttg cactggggct tccggccaac ttcatccgca ccggcaggct caagcagcgg
     1921 gatggcgcaa ggccgcttgc aaaggccggt ggccttgcag ccgtgctggc tggcagctgc
     1981 tcggccgcga ccctcgcgca ggttgctgcc atgcgtgcaa aggctccggc cttcgcgctg
     2041 gatcccatgg ccctgcacgc cgatcgtggc gccgctgtcg cggaggccct cgcctgggcc
     2101 gcgtcacgac tggaaaaggg cccgatcctg attcattcca cggccgagcc cgaggccgtg
     2161 tcgcgcgccc aggccgcact cggacgcgag gcggctggcg cattggtgga agagtccatt
     2221 gcggccattg cccgggagct ggtggcgcgc ggcgtgcgcc gcctcgtggt ggcgggtggc
     2281 gagacctccg gcgccgtcgt atcggcgctt ggcgtcacgg cgctcgagat cggcccgcag
     2341 atcgcacccg gcgttcccgc cacgctgtcc tatggcgaac cccgccttgc gctcgcgctc
     2401 aagtcgggca acttcggcgg gcccgagttc ttcaccgaag cgctggaggc cctgcagtga
     2461 acgaaaccca ggcccgcgaa ttcatcgccg aaatcggccg ctccatgttc gagcggcggt
     2521 tgaccttcgg aagttccgga aacatcagtg tacggatcga cggtggctgg ctgatgaccc
     2581 ccaccggttc ctcgctcggg cgcctcgatc cg
//
LOCUS       DBNGVR010000045         3474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_212991, whole genome shotgun sequence.
ACCESSION   DBNGVR010000045 DBNGVR010000000
VERSION     DBNGVR010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_212991"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="AC16A0_00920"
     CDS             <1..334
                     /locus_tag="AC16A0_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR3103916.1"
                     /translation="LGQGAQVLATGGGAFMNDETRARIAAEGVSVWLRAELPLLMKRV
                     AKRPGRPLLQERDPETVMRELIGKRYPIYGLADVTVESRDVQHGQMVNDVVRALAQWD
                     GWERWKND"
     gene            327..1454
                     /gene="aroB"
                     /locus_tag="AC16A0_00925"
     CDS             327..1454
                     /gene="aroB"
                     /locus_tag="AC16A0_00925"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3103917.1"
                     /translation="MTDQENLQTVAVALGNRSYDIVIGEGLLSGAGRIIGPLLRRPMT
                     AIVTDRNVAEAHLRTLQDSLAAAGVASHAVVVPPGEGTKTFTQLAHVCDELLAAGIER
                     RDRIVALGGGVIGDLAGFAAAVLRRGVDFIQIPTSLLAQVDSSVGGKTGINSIHGKNL
                     IGAFHQPVAVITDIGLLDTLPRRELAAGYAEVAKYGLLGDESFFSWLESSAELVMRGD
                     RQARIHAIRRSCEAKAAIVAEDETETGVRALLNLGHTFGHALEAATGYSARLLHGEGV
                     AIGMVQAFRFSESLGLCAAGTGDRVARHLKSVGLPVHASEIPGDLPPPEALVEIMRQD
                     KKAQGGKLTFILVRGIGEAFIARDVADADVIAFLKGDLQRR"
     gene            1451..2740
                     /locus_tag="AC16A0_00930"
     CDS             1451..2740
                     /locus_tag="AC16A0_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR3103918.1"
                     /translation="MTLDLGLSIAAILILLVCSAFFSGSETALTATSRARLTELERRG
                     SLRARMVLTLTSMRERLIGALLLGNNVVNISASALATLVLVRMFGDTGAVIASVIMTI
                     LVLIFGEVLPKTYAIANPDRAALAAAPLVRVIVAVFGPIVMAVEFIVKQVLRLFGVDI
                     KASQNVLSAHAELRGAIDLHHKEGGVVKGDRDMLGGILDMQDLEVSDVMVHRTRMTMI
                     DADEEPAEIVAHMLKSGHTRIPVWTETPDNIIGVLHAKNLFAALQKQGGDARKVKIDE
                     ILSPAWFVPDTRPLPDQLNAFLKRKTHFAIVVDEYGEVQGLITLEDIIEEIVGDIKDE
                     HDAVASGVRQKPDGSFLVEGGVPIRDLNRAFDWKLPDEEFTTIAGLVIHEARMIPDVG
                     QAFNFHGFRFEILKKRKHQVTAIRVTPLQQAEGLAAG"
     gene            2727..>3474
                     /locus_tag="AC16A0_00935"
     CDS             2727..>3474
                     /locus_tag="AC16A0_00935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3103919.1"
                     /translation="MRQVDLVIVGASFAGLICARAAALRGLRTLVIEAKPEPGARIHT
                     TGILVKEAADEIDTPAALSRSIQGVRLYAPSLKHIDLASRDYYFQTTDTAALIRWLAD
                     EARRAGAEIVCGRKFAHAVRTGTGFDLPQFEVATRFLVGADGARSTVARLFQLGRNTR
                     FLTGLEIEYEELPTADPDLLHCFLDSRLARGYLAWVAPAPGFVQVGLATSEGTKPDLK
                     AFLAHAEALFGFSQARIRERRSGLIPCGGPV"
BASE COUNT          601 a         1164 c         1101 g          608 t
ORIGIN      
        1 gctcggacag ggtgcgcagg ttctggccac cggcggcggt gccttcatga atgacgagac
       61 cagggcacgg attgccgccg agggcgtctc ggtctggctg cgtgccgaac tgccgctgct
      121 gatgaaacgc gtggccaagc ggccggggcg gccgctgctg caggagcgcg acccggaaac
      181 cgtgatgcgc gagctgatcg gaaagcgcta cccgatctat ggcctggcgg acgtcacggt
      241 cgaaagccgc gacgtccagc acggacaaat ggtcaatgac gttgtccgcg ccctggcgca
      301 gtgggacggc tgggagcggt ggaagaatga ctgaccagga aaacctccag accgtggctg
      361 tcgcactcgg caaccgcagc tacgacatcg tcatcggcga aggcctgctg tccggtgccg
      421 gccgcatcat cggccccctg ctgcggcggc cgatgaccgc catcgtcacc gaccgcaacg
      481 tcgcggaagc ccacctgcga accctgcagg actcgctggc cgccgcaggc gtcgcttcgc
      541 atgcggtcgt cgtgccgccg ggcgagggaa ccaagacctt cacgcagctt gcccatgtct
      601 gcgacgaatt gctggctgcc ggcatcgaac ggcgcgaccg catcgtcgcc ctgggcggcg
      661 gggtgatcgg agatctcgcg ggattcgcgg cagcggtcct ccgccgcggc gtggatttca
      721 tccagatccc gaccagcctt ctggcccagg tcgactcttc cgtcggcggc aagaccggca
      781 tcaattccat ccatggcaag aatctgatcg gcgccttcca tcagccggtg gcggtgatca
      841 ccgacattgg cctgctcgac acgctgccgc ggcgcgaact ggctgcaggt tatgccgaag
      901 tcgccaagta cggtctgctg ggcgatgaat ctttcttcag ctggctggaa tcaagcgcag
      961 agcttgtcat gcgcggggac cggcaggcgc gcattcatgc tatccgccgc tcctgcgaag
     1021 ccaaggctgc gatcgtggcc gaggacgaga ccgaaacagg tgtgcgggca ctgctcaatc
     1081 ttggccatac cttcgggcat gcgctggagg cggccacggg ctattccgcc aggctgctac
     1141 atggcgaagg cgtggccatc ggcatggtgc aggccttccg cttctccgag tcgctcggtc
     1201 tctgcgcggc gggaacggga gatcgcgtgg cccggcatct caagagcgtc ggcctgccgg
     1261 ttcatgcttc cgaaattccc ggcgacctgc cgccgcccga ggctctggtc gaaatcatgc
     1321 gtcaggacaa gaaagcccag ggcggcaagc tcaccttcat cctggtgcgc ggcattggcg
     1381 aagccttcat cgcccgcgat gtcgccgacg ccgacgtgat cgccttcctg aagggagacc
     1441 tgcagcgtcg atgacactcg atctcggact ctcaatcgcc gccatactga tcctgctcgt
     1501 ttgctcggcc ttcttctcgg gatccgaaac cgcgctcacc gcaacctccc gtgcccgcct
     1561 caccgaactc gagcggcgcg gcagcctgcg cgcccgcatg gtgctgacgc tgacgagcat
     1621 gcgcgagcgc ctgatcggcg cgctgctgct gggcaacaat gtggtgaaca tctcggcctc
     1681 ggcgctggcc acgctggtgc tggtgcgcat gttcggcgac acgggtgccg tcatcgcatc
     1741 ggtgatcatg acgatcctgg ttctcatctt cggtgaggtc ctgccgaaga cctatgccat
     1801 cgccaatccc gaccgtgccg cgctggccgc agcacccctg gtgcgcgtca tcgtggcagt
     1861 cttcggcccc atcgtcatgg cggtggagtt catcgtcaag caggtgctgc gcctcttcgg
     1921 cgtcgacatc aaggcctcgc agaacgtgct ttcggcccat gccgaactgc gcggcgccat
     1981 cgaccttcat cacaaggaag gcggcgtggt gaagggcgac cgcgacatgc tgggcggcat
     2041 tctcgacatg caggacctcg aggtttccga cgtcatggtt caccgcaccc gcatgaccat
     2101 gatcgatgcc gatgaggaac ccgcagagat cgtggcccac atgctgaaaa gcggccatac
     2161 ccgcattccg gtctggaccg agacgcccga caacatcatc ggcgtgctgc atgccaagaa
     2221 tctcttcgcc gcgctgcaaa agcagggcgg cgatgcgcgc aaggtaaaga tcgacgagat
     2281 cctgagcccc gcatggttcg tgcccgacac gcgcccgctg cccgatcagc tcaatgcgtt
     2341 cctcaagcgc aagacgcatt ttgccatcgt cgtcgacgaa tatggtgagg tacagggact
     2401 gatcacgctc gaagacatca tcgaggagat cgtcggcgac atcaaggacg agcacgatgc
     2461 cgtggccagc ggtgtgcgcc agaagccgga cgggtccttc ctcgtcgagg gtggcgtgcc
     2521 gatccgcgac ctcaaccgcg ccttcgactg gaagcttccg gatgaagaat tcaccaccat
     2581 cgccggtctg gtcattcacg aggcccgcat gatcccggac gttggccagg cattcaactt
     2641 ccacggcttc cgcttcgaga tcctcaagaa gcgcaagcac caggtcaccg caatccgcgt
     2701 cacgccgctg cagcaggccg aaggccttgc ggcaggttga cctcgtcatt gtcggtgcca
     2761 gtttcgccgg cctgatctgc gcgcgcgcgg cggcgcttcg cggccttcgt accctggtga
     2821 tcgaggccaa gcccgaaccg ggtgcacgca tccacaccac cggcatcctg gtcaaggagg
     2881 cggccgacga gatcgacacg cccgcagcgt tgtcccgctc aattcagggc gtcaggctct
     2941 atgcaccatc gctaaagcat atcgacctcg cctcgcgcga ctactacttc cagaccaccg
     3001 acacggcggc gctgatccgc tggctggccg acgaagcgcg ccgcgctggc gcggagatcg
     3061 tctgcgggcg gaagttcgcg catgccgtcc gaaccgggac cggcttcgac ctgccacagt
     3121 tcgaggtggc gacgcgcttc ctcgtcggcg ccgacggagc ccgctcgacc gtcgcgaggc
     3181 tctttcagct cgggcgcaac acgcgcttcc tcaccgggct ggaaatcgag tatgaggaac
     3241 ttcccactgc cgatccggat ctcctccatt gcttcctcga ttcgcgcctg gcgcggggct
     3301 atttggcctg ggttgcgccg gcgccgggat tcgttcaggt gggtctagcc acaagcgagg
     3361 gaaccaagcc cgatctcaag gccttccttg cccatgccga ggcactcttc ggattctcgc
     3421 aggcccggat cagggaacgc cgcagcgggc tcattccatg cgggggaccg gtct
//
LOCUS       DBNGVR010000046         6310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3552445, whole genome shotgun sequence.
ACCESSION   DBNGVR010000046 DBNGVR010000000
VERSION     DBNGVR010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3552445"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="AC16A0_00940"
     CDS             complement(<1..610)
                     /locus_tag="AC16A0_00940"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3103920.1"
                     /translation="MTQTSPRVGLFVTCLVDLIRPSIGFAAVKLLEDAGCTVEVPEAQ
                     TCCGQPAFNAGDRNTAADLARNVIAAFKGYDYVVAPSGSCGGMIVKHYPELFTEGSVD
                     HAAAVDLASRSHELVSFLTRVMKVTAVDAKFPAKATYHDSCSGLRELGIRKEPRQLLA
                     SVEGVTLKEMTDAEVCCGFGGTFCVKYPGISDKMLEKKLKHIS"
     gene            complement(682..1059)
                     /locus_tag="AC16A0_00945"
     CDS             complement(682..1059)
                     /locus_tag="AC16A0_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3103921.1"
                     /translation="MKKSVPLALALLASLSLPALAEGDPAKGAKVFNKCKACHVADAD
                     TNKVGPTLKGLIGRNTASVPGYKYSEAMLEYAKANPVWDDAHFLKYIEDPKGVVPKTK
                     MAFAGLKKEDERVDLLAYLKTMK"
     gene            1159..2649
                     /locus_tag="AC16A0_00950"
     CDS             1159..2649
                     /locus_tag="AC16A0_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3103922.1"
                     /translation="MLKMPIPDAAAIGRRAEIAAALRRIVPGEGVIEAPVEMRAYETD
                     GLTAYRQLPLVVVLPETTDQVAQVLRYAYENAIKVVPRGAGTSLSGGALPLADGILLG
                     MARFNRILDIDFANRCAVVQPGVTNLGITRAVEHRGFYYAPDPSSQIACSIGGNVAEN
                     SGGVHCLKYGLTANNVLGIEMVLITGEVIRLGGKHLDSEGYDLLGLMTGSEGLLGVVT
                     EVTVRILQKPETARAVLVGFPTSEDAGQCVADIISAGIIPGGMEMMDRPAIHAAEDFV
                     HADYPLDVEALLIVELDGPKVEVDQLIARVSEIARQNRSSTLRISESEEERLLFWAGR
                     KAAFPAVGRISPDYLCMDGTIPRKKLPEVLRGMAELALQYDLRVANVFHAGDGNLHPL
                     ILFDANDPDQLERAENFGADILRLCVRVGGVLTGEHGVGIEKRDLMPEMFSEIDLNQQ
                     QRVKCAFDDQGLLNPGKVFPTLHRCAELGRMHVRGGRLPFADIPRF"
     gene            2661..3800
                     /locus_tag="AC16A0_00955"
     CDS             2661..3800
                     /locus_tag="AC16A0_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3103923.1"
                     /translation="MKPGSEAELAQLVAQAEAPFEIIGTGTKRGLGRPVQAARTLDMS
                     AFAEIAAYEPEELILEAGAGTRLAEIEALIAARGQQLAFEPPDLSRLLGSPHSGTLGG
                     LMACNLAGPRRIKAGAARDHVLGIRGVSGRGEVFKAGARVVKNVTGYDLPKLLSGSYG
                     TLAALTSITVKVLPRPETEETILVEGLDDTAAVSVMSLAMQSACEVSGAAHVPWEGTF
                     LRLEGIAASVAARRDVLAKLLARPVQILAAKASGTIWQRIRDVLDLADTPDRLVWRLS
                     VTPSDAPALLAILRGSLEIRYVLDWAGGLIWLDVPPTEDAAAAVIRAAMRSGHATLIR
                     APEGVRAAVDVFHPQAPALAALSARVKDSFDPRRLFNPGRMSRGL"
     gene            3800..5098
                     /gene="glcF"
                     /locus_tag="AC16A0_00960"
     CDS             3800..5098
                     /gene="glcF"
                     /locus_tag="AC16A0_00960"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HIR3103924.1"
                     /translation="MQTSFTAEQLEQPRIAEANTILRKCVHCGFCTATCPTYVLLGDE
                     LDSPRGRIYLIKNMLETGGPATAETVKHIDRCLSCLSCMTTCPSGVNYMHLVDEARAH
                     VEETYSRPLFDRALRAVLAYAMPRPRLFRLALIAARMARPLRPVVQSFGGTRIDALLG
                     LVPAALPTPERFGRPGTYPAAGERKGRVALLMGCVQSVLDPGINAATIRLLTRLGIEV
                     VVSGGEACCGSLTHHMGKESDALARARRSVDQWTAASVDAVVITASGCGTTIKDYGHM
                     LRLDPAYAERAAAVSAKAKDVTEFLAGLDLPDGQTSLRLAYHSACSMQHGQKIRTEPL
                     KLLRKAGFTVAEVPEGHLCCGSAGTYNILQPDIATRLRDRKVANIMSTAPQAVATGNI
                     GCISQISTGTDIPVVHTVELLDWAYGGPIPEKLKPICGSH"
     gene            5101..>6310
                     /gene="aceB"
                     /locus_tag="AC16A0_00965"
     CDS             5101..>6310
                     /gene="aceB"
                     /locus_tag="AC16A0_00965"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR3103925.1"
                     /translation="MAKLPKGVTFTVKLAKAHERVLTPDALNFVASLHRRFEAERRTL
                     LARRAVRQKAFDAGLLPDFLPETASIRAGDWKVAPIPHDLQDRRVEITGPTDRKMMIN
                     ALNSGARVFMADLEDSNSPSWDNMVQGQINLMDRWTSAMVHVDPVSGKRYALGQRLAV
                     LMVRPRGWHLDEAHMKVDGKPVAAALFDFGLYAFHNARTAIAKGSGHYFYLPKIESHL
                     EARLWNGVFVFTQSWLGLPLGSIKATVLIETLPAAFEMDEIIYELRDHLTGLNCGRWD
                     YIFSFIKTLAAHKTFLLPDRAQLVMAEAFLAAYSALLVKTCHRRGCFAMGGMAAEIPN
                     RKDPEANGLAFARVRADKEHEARNGHDGTWVAHPELVPLAMEVFNDLMPTPNQRYVMR
                     DDAQVGQKDLL"
BASE COUNT         1067 a         2090 c         2066 g         1087 t
ORIGIN      
        1 ccgagatgtg cttgagcttc ttctccagca tcttgtccga gatgccggga tacttcacgc
       61 agaaggtgcc gccgaagccg cagcagacct cggcgtcggt catttccttc aacgtcacgc
      121 cctcgacgct ggccagaagc tgacgcggct ccttgcgaat gccaagctcg cgcaggcccg
      181 agcaggagtc atgataggtg gccttggccg ggaacttcgc atcgaccgcc gtcaccttca
      241 tcacccgcgt gaggaacgag acgagttcgt ggctgcgcga cgcgaggtcg acggcggccg
      301 cgtggtcgac ggacccctcg gtgaaaagtt ctggatagtg tttcacgatc atgccgccgc
      361 aggagcccga gggcgccacg acatagtcgt agcccttgaa ggcggcaatc acgttgcggg
      421 cgaggtcggc ggcggtgttc cggtcgccgg cgttgaaggc gggctgaccg cagcaggtct
      481 gggcctcggg aacctcgacg gtgcagcccg cgtcttcgag cagtttcacc gcagcgaagc
      541 cgatggatgg ccggatcagg tcgaccaggc aggtcacgaa gaggccgacg cggggagagg
      601 tctgggtcat cagggctcgg ggcaaacgcg tgaggctggc gttttgccag ccccggcgct
      661 gccccgcaag ccgtttcgcg gttacttcat ggtcttgaga taggcgagaa ggtcgacacg
      721 ttcgtcttcc ttcttcaggc cggcgaaggc catcttggtc ttggggacaa cgcccttcgg
      781 atcctcgatg tacttcagga aatgcgcgtc gtcccagacg ggattggcct tggcgtattc
      841 gagcatggcc tcggagtact tgtagccggg gaccgaggcg gtgttgcgcc cgatcaggcc
      901 cttgagcgtc ggaccgacct tgttggtgtc ggcatcggcc acgtggcagg ccttgcactt
      961 gttgaaaacc ttggcccctt tggccgggtc gccttcggca agggccggca ggctaaggct
     1021 tgccagcagg gcgagggcaa ggggaacgga cttcttcatc ttggttgctc caggaaacgg
     1081 cgcggattgc gctttgaacg gaaacggatt tcagtacaag atagtgtcga aagcgtgaaa
     1141 cggatcacct gccgaccaat gctcaagatg cccattccag atgccgccgc aatagggcga
     1201 agggccgaaa ttgccgctgc acttcgacgc atcgtgccag gcgaaggggt gatcgaggcg
     1261 ccggtcgaga tgcgggccta tgaaaccgac gggctgaccg cctaccggca gctgccgctg
     1321 gtggtggtcc tgcccgagac gacggaccag gtggcgcagg tgctgcgcta tgcttatgaa
     1381 aatgcaatca aggtggtccc ccgcggggcc ggaacctcgc tgtcgggtgg cgccctgccg
     1441 ctggccgacg gcatcctgct gggcatggcc cgtttcaatc ggatcctgga catcgacttc
     1501 gcaaaccgct gcgccgtggt gcagccgggc gtgaccaacc tcggcatcac ccgggcggtg
     1561 gagcaccggg ggttttatta cgcccccgac ccttcctcgc agatcgcctg ctccatcggc
     1621 ggcaatgtgg ccgagaattc cggcggcgtt cactgtctca aatacgggct caccgccaac
     1681 aacgtgctgg gcatcgagat ggtgctgatc accggcgagg tgatccggct gggcggcaag
     1741 catctggaca gcgagggcta tgacctgctg ggcctgatga ccgggtcgga gggccttctg
     1801 ggtgtcgtca cggaagtgac cgtccgcatc ctgcagaagc ccgagacggc ccgcgccgta
     1861 ttggtcggct ttccgaccag cgaagatgcc gggcaatgcg tggccgacat catctcggct
     1921 ggaatcattc cgggcgggat ggaaatgatg gaccggcccg ccatccacgc ggccgaggat
     1981 ttcgtccatg ccgactatcc gctcgatgtc gaggcgctcc tcatcgtcga actcgacggg
     2041 ccgaaagtgg aagtggacca gctcatcgcg cgcgtgtctg agattgcgcg gcaaaacagg
     2101 tccagcacct tgcgcatctc cgagtcggag gaggagcgcc tgctcttctg ggccgggcgc
     2161 aaggcggcct tccccgccgt gggacgcatc tcgccggact acctctgcat ggacggaacg
     2221 atcccgcgga aaaaactgcc cgaggtgttg cgcggcatgg cggagctcgc gcttcagtat
     2281 gacctgcgcg tggccaatgt ctttcatgcc ggcgacggca acctgcatcc gctgatcctc
     2341 ttcgatgcca acgatcccga ccagctcgaa cgcgccgaga atttcggcgc cgacatcctt
     2401 cggctttgcg tgcgcgtggg cggcgtgctg acgggcgagc acggcgtggg catcgagaag
     2461 cgcgacctca tgccggagat gttctcggag atcgacctca accagcagca gcgggtgaaa
     2521 tgcgcctttg acgaccaggg gcttctcaat cccggcaagg tctttcccac gctgcatcgc
     2581 tgcgccgaac tggggcggat gcatgtgcgc ggcggccggc tgcccttcgc cgacattccg
     2641 agattctgat ggaagcagcc gtgaaaccgg gctcggaggc cgagcttgcg cagctcgtgg
     2701 cacaggctga ggcgcctttc gagatcatcg gcaccggtac gaagcgcgga cttggccgtc
     2761 cggtgcaggc ggcgcggacc ctcgacatgt cggccttcgc ggagatcgcc gcttatgagc
     2821 cggaagaact cattcttgag gccggcgccg gaacacggct cgccgagatc gaggcgctga
     2881 tcgccgcgcg cggccagcaa cttgccttcg aaccgccgga cctgtcgcgc ctgctgggga
     2941 gccctcacag tggcacactg ggcgggctca tggcctgcaa cctcgcgggg ccgcgccgca
     3001 tcaaggccgg cgctgcgcgc gatcacgtgc tgggcatccg cggcgtctcg ggccgcggcg
     3061 aggtcttcaa ggccggcgcc cgcgtggtga agaacgtcac cggctatgac ctgccaaagc
     3121 tgctgtcagg ctcctacggc acgctggcgg ccttgacctc catcaccgta aaggtgctgc
     3181 cccggccgga gacggaggaa accatcctcg tcgaaggcct cgacgacacc gcggccgttt
     3241 cggtcatgag cctcgccatg caatcggcct gcgaagtctc cggcgccgcc catgtgccgt
     3301 gggaaggaac tttcctgcgg ctcgagggga tcgcagcatc ggtggcggcg cggcgcgatg
     3361 tgctggccaa gctgctggca cgtcccgttc agatcctcgc ggccaaggcc tctggcacga
     3421 tttggcagcg cattcgcgac gttctggatc ttgccgacac accggaccgc ttggtctggc
     3481 gcctctccgt cacgccgtcg gacgcgcccg cgctgctggc gatactgcgc gggagcctgg
     3541 aaatccgata tgtcctcgat tgggccggcg ggctcatctg gctcgatgtt ccgcctacgg
     3601 aggatgcggc ggcggccgtc attcgcgccg cgatgcggtc gggccatgcc accctgatcc
     3661 gcgcccccga aggcgtgaga gcggccgtcg acgtgttcca cccgcaggcg ccggcgctgg
     3721 cggcgctctc ggcccgcgtg aaggactcct tcgacccgcg ccgcctgttc aatcccggcc
     3781 gcatgtcgcg aggcctctga tgcagacgtc cttcaccgcc gaacagctcg aacagccgcg
     3841 catcgcggaa gccaacacga tcctgcggaa atgcgtgcat tgcggcttct gcaccgccac
     3901 ctgccccacc tatgtgctgc tcggcgacga gctcgacagc ccgcgcggcc gcatctacct
     3961 gatcaagaac atgctcgaaa cgggtggccc ggccacggcc gaaacggtga aacacatcga
     4021 ccgctgcctc tcctgcctgt cctgcatgac cacctgcccg tccggcgtga actacatgca
     4081 tctcgtcgac gaggcccgcg cccatgtcga agagacctac agccggccgc tgttcgaccg
     4141 cgcgctcagg gccgtgcttg cctatgccat gccgcggccg cggctgttcc gcctggcgct
     4201 gatcgcggcc cgcatggcgc ggccattgcg ccccgtggtg caatcctttg gcggcacccg
     4261 catcgacgcg ttgctgggcc tcgtgcccgc cgcgctgccc acgcccgagc gcttcggccg
     4321 gcccggcacc tatccggctg ccggcgaaag gaagggccgc gtcgcgcttc tcatgggctg
     4381 cgtgcagagc gtgctcgacc ccggcatcaa tgccgcaacc atccgcctgc tgacgcgcct
     4441 tggcatagag gtggtggtct cgggcgggga agcctgctgc ggttcgctca cccaccacat
     4501 gggcaaggag tccgatgcgc tggcccgggc aaggcgctcg gtggaccagt ggaccgcggc
     4561 gagtgtcgat gccgtggtga tcacggcatc gggctgcggc accaccatca aggactatgg
     4621 ccacatgctg cgcctcgatc cggcctacgc cgaaagggcc gcggcggtca gcgccaaggc
     4681 gaaggacgta accgagttcc tcgccggtct cgaccttccg gatggccaga cgtcgctccg
     4741 gcttgcctat cactcggcct gctccatgca gcatggccag aagatccgca ccgaaccgct
     4801 gaagctcttg cgcaaggcgg gcttcacggt cgccgaagtg ccggaaggcc atctctgctg
     4861 cggctcggcc ggcacctaca acatcctgca gccggacatc gccacccggc tgcgtgaccg
     4921 caaggtggcc aacatcatgt ccacggcacc gcaggcggtg gccaccggca atatcggctg
     4981 catttcgcag atatccaccg gcacggacat ccccgtggtc catacggtgg aactgcttga
     5041 ctgggcctat ggcggcccta ttccggagaa gctgaagcca atctgcggga gtcactagac
     5101 atggcgaagc tgcccaaggg tgtcaccttc acggtgaagc tggccaaggc gcatgagcgg
     5161 gtcctgaccc ctgacgccct gaacttcgtt gccagcctgc atcgccgctt cgaggcggag
     5221 cgccggaccc tgctggcccg ccgggcggtc cgccagaagg ccttcgacgc cggccttctg
     5281 ccagacttcc tccccgagac ggcgtcgatc cgtgcgggtg actggaaggt ggcacccata
     5341 ccgcacgacc tgcaggaccg gcgcgtcgag atcaccggcc ccaccgatcg caagatgatg
     5401 atcaatgcgc tgaactccgg tgcacgggtg ttcatggccg acctggagga ttccaattcg
     5461 ccgtcctggg acaacatggt ccagggccag atcaacctga tggaccgctg gaccagcgcc
     5521 atggtccatg tcgatccggt cagcggcaag cgctatgcgc tgggccagag gctggcggtg
     5581 ctgatggtgc ggccacgcgg ctggcatctc gacgaggccc acatgaaggt ggacggcaag
     5641 ccggtggcgg ccgcgctctt cgatttcggc ctctatgcct tccacaatgc gcgaaccgcg
     5701 atcgccaagg gatcggggca ctacttctac ctgcccaaga tcgagagcca tctcgaggca
     5761 cgcctgtgga acggcgtgtt cgtcttcacg cagagctggc tgggtctgcc gctcggctcc
     5821 atcaaggcca cggtgctgat cgagaccctg ccggcggcct tcgagatgga cgagatcatc
     5881 tacgagctcc gcgatcacct gaccggcctc aactgcggcc gctgggacta catcttctcc
     5941 ttcatcaaga ccctcgcagc ccacaagacc ttcctgctgc ccgaccgtgc ccagctcgtc
     6001 atggcggagg cgttcctcgc cgcctattcg gcgctgctgg taaagacctg ccatcgccgc
     6061 ggctgcttcg ccatgggcgg catggcggcc gagattccga accgcaagga tccggaggcc
     6121 aacggtcttg ccttcgcccg cgtgcgcgcc gacaaggaac acgaagcgcg caacggacat
     6181 gacggcacgt gggtcgccca tccggaactc gtgccgctgg cgatggaagt cttcaatgac
     6241 ctcatgccaa cgccgaacca gcgctatgtg atgcgtgacg atgcccaagt gggacagaag
     6301 gacctgttgc
//
LOCUS       DBNGVR010000047         3652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3356292, whole genome shotgun sequence.
ACCESSION   DBNGVR010000047 DBNGVR010000000
VERSION     DBNGVR010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3356292"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16A0_00970"
     CDS             <1..482
                     /locus_tag="AC16A0_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3103926.1"
                     /translation="TVICSDMTQIAYAANEVFPVSQPRTWLHPVGFGTLGFALPAGIG
                     AKFGVGARPVAVMIGDYGLQYTINELGTAAEHRLPLVILMWNNDALGQIRDDMVNKGI
                     QPNAVTLKNPDFQMLARSYGCQAAKPESLAALSAAIRTALAAEGPTLIEMTPRMVNG"
     gene            475..1143
                     /locus_tag="AC16A0_00975"
     CDS             475..1143
                     /locus_tag="AC16A0_00975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3103927.1"
                     /translation="MDSAVIFDVDGVLLDLTPAEEDGFFVPFARRYGLTGLSRDWDSY
                     RTRNDDDIIAEILATNGLPPSDRDDVIADYLEVMASGLRARHFSPVEIAGARALLQSL
                     HGRHAIGIATANLLAAARLRLIHAGLWQWVEGYAFGAEGGGHKRETVARAIAATGLPR
                     TRIVYIGDNLNDVVAGLENGVHFIGFAVDAARRHRLSAAGAEYTSGDHATTRDMIARL
                     LSKD"
     gene            complement(1202..1690)
                     /locus_tag="AC16A0_00980"
     CDS             complement(1202..1690)
                     /locus_tag="AC16A0_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR3103928.1"
                     /translation="MRSFRNLLAAGAIAVSSLVATSQAFAADIVDTAVGAGSFKTLVA
                     AVQAAGLVDTLKGAGPFTVFAPSDEAFAKLPAGTVEDLLKPENKDKLVAILTYHVVPG
                     KVMAADVAGKETMAKSVQGGEIKVNGTNGVMVNDAKVVQADIVADNGVIHVIDAVIMP
                     PM"
     gene            1863..>3652
                     /locus_tag="AC16A0_00985"
     CDS             1863..>3652
                     /locus_tag="AC16A0_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3103929.1"
                     /translation="MSEDNQLALLKSRSFLPLFLTQAISAFNDNALRNGIAILITFDL
                     AVRHGWNATLFVQAGTALFMLPYFMFSAIAGQLADKFDKAMLARRIKIFDLAAMVFGA
                     LSLWLENPFMHLMVLFLAGTTAAFFGPIKYGILPQYLKRQDLIGGNALIELGTFVTIL
                     LGTMFGGFLVLHDTGRLVLAGTIVALALIALFAAFRMPPAPGQPSLAFDWNVPRQTMK
                     LLGYAHERIDVFWSVLGASWFWFLGTAILVQFPIFTKDILLSNDYVANIFIATFTIGI
                     GAGSMLTNTLLGGQVSARYTPIAAIMMTVFLVDLYFSSGTVNELFAGTTMNTAGELFS
                     HWSGWRTGFDLFMVAFFGGIYAVPLNAIMQQRSNPTRRARVIAANNVINAVFMIAATL
                     ISVVLLQIMTPRGLFLCLAIANGFVAVWICRLLPQEFVASIARQVFKLLYRVEVKGIE
                     NFNAVGRKAVIVANHTSFLDGPILSAYLPERCGFAINTNMSKRWWVKPAHFLFDMVPI
                     DPTNPLAIRTLVEEVKKGRKIVIFPEGRITVTGTLMKVYEGPGAIAQMANAAVLPVRI
                     DGAQFSPFSRLRGKVRLRWFPRITLTFLPPV"
BASE COUNT          604 a         1184 c         1127 g          737 t
ORIGIN      
        1 agaccgtgat ctgttccgac atgacccaga tcgcctatgc cgccaacgag gtctttccgg
       61 tctcgcagcc gcggacctgg ctgcatccgg tgggcttcgg cacgctgggt ttcgcgctgc
      121 ccgcaggcat tggcgccaag ttcggtgtgg gtgcgcggcc ggtggcggtg atgatcggcg
      181 actacggcct gcaatacacc atcaacgagc ttggcactgc ggccgaacac aggctgccgc
      241 tggtcatcct catgtggaac aacgatgcgc tgggccagat ccgcgacgac atggtgaaca
      301 agggcatcca gcccaatgcc gtgacgctga agaatccgga cttccagatg ctggcccggt
      361 cctatggctg ccaggcggca aagcccgaaa gccttgcggc gctgtcggcg gcgatccgga
      421 cggcgctcgc tgcagagggc ccgacgctga tcgagatgac gccgcggatg gtgaatggat
      481 agtgccgtca tcttcgacgt cgatggcgtt ctcctcgatc tgacgccggc ggaggaagat
      541 gggttcttcg ttccctttgc gcggcggtac gggctgaccg gcctgtcgcg cgactgggac
      601 agctaccgca cccgtaatga cgatgacatc atcgccgaga tcctcgcgac caacggcctg
      661 ccgccctcgg accgcgacga cgtgatcgcc gactatctgg aggtgatggc ctcggggctt
      721 cgcgcgcggc atttcagccc ggtcgagatt gccggagccc gggccctgct gcaatcgctg
      781 cacggccgcc atgccattgg catcgcgacc gccaacctgc tggccgcggc gcgcctccgc
      841 ctcatccatg ccggactctg gcaatgggtc gaaggctatg ccttcggtgc ggaaggcggc
      901 ggccacaagc gcgagacggt cgcgcgggcg atcgctgcca ccgggcttcc aaggacacgc
      961 attgtctata ttggcgacaa tctcaacgat gtcgtggccg ggctcgaaaa tggtgtccac
     1021 ttcatcgggt ttgcggtgga tgccgcgcga agacatcgcc tgtctgcggc cggcgcggaa
     1081 tacacaagcg gcgaccatgc caccacgcgt gacatgatcg cccgcctgct ttcgaaggat
     1141 tgaggggccg gagatccggg ggagggcgcc agcccctcgg gcagcggagg agctgccgtg
     1201 cttacatcgg cggcatgatc acggcgtcga tcacatggat cacgccgttg tcggccacga
     1261 tatcggcctg caccaccttg gcgtcgttga ccatcacgcc gttggtgccg ttgaccttga
     1321 tctcgccacc ctggacggac ttggccatgg tttccttgcc cgccacgtca gcggccatca
     1381 ccttgcccgg aacgacatgg taggtcagga tcgcgacgag cttgtccttg ttctcgggct
     1441 tcagcaggtc ctcgaccgtt ccggccggga gcttggcgaa ggcttcatcg ctgggcgcga
     1501 agacggtgaa ggggccggcg cccttcaggg tgtcgacaag gcccgcagcc tgaacggcgg
     1561 cgaccagggt cttgaagctg ccggcgccga cagcggtgtc gacgatatcg gcggcgaagg
     1621 cctgcgaggt ggcgacaagg ctggatacgg cgatggcgcc ggcggcaaga aggttacgga
     1681 agctgcgcat tgtagtccct tttggttgtg gggttgcagg aggtcttacg ccagggccgg
     1741 gaatgcggtt ctcgaggccg ggttcacaat aagtttttca ggctttgatg atccgtctca
     1801 ggtctgacat attcctgaag acatctgaat catctccgcg ttccgcaagg tttccaaggc
     1861 tcatgagcga agacaaccaa cttgccctgc tgaaaagccg gagttttctg ccgctgttcc
     1921 ttacccaggc gatcagcgcc ttcaacgata acgccctgcg caacggcatc gccatcctga
     1981 tcactttcga cctggcggtc cggcatggct ggaacgccac gctcttcgtg caggccggca
     2041 cggcgctgtt catgctgccc tacttcatgt tctcggccat cgccggtcag ctggccgaca
     2101 aattcgacaa ggccatgctg gcgcgtcgca tcaagatctt cgatctggct gccatggtct
     2161 tcggtgcgct cagcctgtgg cttgagaacc ccttcatgca tctgatggtg ctgttcctcg
     2221 ccggcacgac cgccgccttc ttcggcccga tcaagtacgg aatcctgccg cagtacctga
     2281 agcggcagga tctcatcggc ggcaatgcgc tgatcgagct cggcaccttc gtcaccatcc
     2341 tgctgggcac catgttcggt ggcttcctcg tcctgcacga caccggccgg ctggtgctgg
     2401 ccggcaccat cgtggccctg gcgctgattg cgctcttcgc cgccttccgc atgccaccgg
     2461 cgccgggaca gcccagcctc gccttcgact ggaacgtgcc gcgccagacc atgaagctgc
     2521 tgggctatgc gcatgaacga atcgacgtct tttggtcggt gctcggcgcc tcgtggttct
     2581 ggttcctcgg caccgccatc ctggtgcagt tcccgatctt cacgaaggat atccttctct
     2641 ccaacgacta tgtggccaac atcttcatcg ccaccttcac catcggcatc ggcgccggtt
     2701 cgatgctgac caatacgctg ctgggcggtc aggtttcggc gcgctacacg ccgatcgccg
     2761 ccatcatgat gacggtcttc ctcgtcgatc tctatttctc ctccggcacc gtcaacgaac
     2821 tctttgccgg cacgaccatg aacacggcag gagagctctt ttcgcattgg tccgggtggc
     2881 gcaccggttt cgatctcttc atggtagcct ttttcggcgg catctatgcc gtgccgctca
     2941 acgccatcat gcagcagcgc tccaacccga cacggcgggc gcgggtcatt gccgccaaca
     3001 acgtgatcaa cgcggtcttc atgatcgcgg caacgctgat ctcggtggtg ctgctgcaga
     3061 tcatgacgcc gcgcgggctg ttcctctgcc tggccatcgc caatggcttt gtcgccgtgt
     3121 ggatttgccg cctgctgccg caggagttcg tggccagcat cgcccgccag gtcttcaagc
     3181 tgctctaccg cgtggaggtg aagggcatcg agaacttcaa tgccgtaggc cgcaaggcgg
     3241 tgatcgtcgc caaccatacg tcattcctcg atggcccgat cctgtcggcc tatctgccgg
     3301 agcgctgcgg ctttgcgatc aataccaaca tgtcgaaacg ctggtgggta aagccggcgc
     3361 atttcctctt tgacatggtg ccgatcgatc cgaccaatcc cctggcgatc cggaccctcg
     3421 tggaagaggt caagaagggc cgcaagatcg tcatcttccc ggaaggccgc atcaccgtca
     3481 ccggcacgct gatgaaggtc tatgagggtc ctggcgccat tgcccagatg gccaatgccg
     3541 ccgtcctgcc ggtgcgcatc gacggggcgc agttctcgcc cttctcgcgc ctgcgcggca
     3601 aggtgcgcct gcgctggttt cccaggatca cgctcacctt cctgccgccg gt
//
LOCUS       DBNGVR010000048         4803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3159843, whole genome shotgun sequence.
ACCESSION   DBNGVR010000048 DBNGVR010000000
VERSION     DBNGVR010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3159843"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC16A0_00990"
     CDS             complement(<1..706)
                     /locus_tag="AC16A0_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3103930.1"
                     /translation="MITVDKVSVSFGGLKAVDGVSFEIPTGRITGLIGPNGAGKTTMF
                     NAIAGHVPVQSGRILLEAIDITRLKPHQRAEKGLARTFQIPQEFSRLTVLENLMAAAA
                     MPLGENVFNVMFRRSRFRGEEEAVYRAARETVTFLELDRVIDEKAGNLSGGQKKLLEL
                     GRALMRNPRIILLDEIGAGINRTLLVKIAEKIKLLNRERGLTFCLIEHDLDYVSRLCD
                     GVVVMAQGRLLMRGTVD"
     gene            complement(746..1948)
                     /locus_tag="AC16A0_00995"
     CDS             complement(746..1948)
                     /locus_tag="AC16A0_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3103931.1"
                     /translation="MKKFTVTIAAVALFGTSALALAADTKVGILMDITGPIANFIPPL
                     QNATNLAVKHVNEQGGLLDGQAIAIFGDTTGSSQGAVDAAGKLINIENVPIIMGALMS
                     GTTIAAAEAAAIPAGVVQISPTATSPAMTGVKDNDLLYRIVPSDNYQGSVLAKMVLDE
                     GIKKVAVTFVNNDYGVGIGQTFIDAYKAAGGEVVAEVKHEEKKDSYRSELATLSKAGG
                     EALVVVAYAGDSGGKIVKQAIEGGLFTRFIGTDGLRDEALIKDVGAEALATSFFSSPT
                     SPADNPNQKTLHDLYNAEFKEGADKAFVDQTYDATFLTLLAIEKAGSADRAKMAAALR
                     EVASAPGEKVGPGEWAKAKALIKEGKDIDYDGAGGAYDFDAAGDVTGYIGKFVVDGDK
                     YKQTAVMQ"
     gene            complement(2050..3153)
                     /locus_tag="AC16A0_01000"
     CDS             complement(2050..3153)
                     /locus_tag="AC16A0_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3103932.1"
                     /translation="MTETRAEIRLPTQELRNDLPFFTKTLKMRLDMIYPADNPQIAVL
                     SGHGLRLRIEKGAREAPGTLRILTDDPDGFADGQRVLTAPNGTRIEIEELDPPLVLPP
                     TQHAFVVRRLADQAPWIIGRAGMEYRDLVPSRLGGAMIASHIRVPDGPVPDMVHFHKV
                     GFQLIFCIRGWVDVLYEDQGGPRRLTAGDCFIQPPTIRHKVLHAADGIEVIEIGVPAE
                     HITEIDHDMTLPTPDLRPEREWDGQRFVHSLAKEGAFKPFRIPGFEARDTTICANTRG
                     VASVVVARPTGQAAPWTRHDGDILFTFVMSGGMVLEGQGKDPYRLASGDAFVIPPGMA
                     TRYGALTPDLQLLEVTLPGAFRTEVLPGPPEDV"
     gene            complement(3172..4407)
                     /locus_tag="AC16A0_01005"
     CDS             complement(3172..4407)
                     /locus_tag="AC16A0_01005"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HIR3103933.1"
                     /translation="MTSTSLELAQALDAKDDLAPIRALFECPDDNIYMDANSVGPMPT
                     AVREKARGLLDDWVHLRRRGWSQRQWLEMPSLLGDCIAPLIGAAPGTVVMSDSTTLNQ
                     FKAVTHALALRPDRKVILTQNGNFPTDVHVLQGIAKASGGRVTLRFVDSEDEAIAAFD
                     DGIAVAALSHVDYRSGERWDMAKVTAAAHRHGALAVWDLSHSAGAVPVDVGAADADLA
                     IGCGYKYLCTGPGGPAYIYLKPGLGDDVWPTLCGWMGHADVFAFARDFAPHSGVKKFL
                     TGTPLVGADELASAILDVWPKVEPAKLWAKHRSLTDFLISALRQECGSLGVTVNSPDD
                     HARRGGNVSFSSPGAGSVVEALIDAGVISSFRKPDAIRFGVSPLVIRHVDCWTAVQRL
                     RRILAEKTWQQPKYEKVSV"
     gene            4492..>4803
                     /locus_tag="AC16A0_01010"
     CDS             4492..>4803
                     /locus_tag="AC16A0_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3103934.1"
                     /translation="MAKQIKLAADIGGTFTDIVLESGAKRWSGKVLTTTAAPELGVIE
                     GIRLVLEQSGLKPADVGVFIHGTTLATNALIERKGARTAFVTTEGFRDILEQGYEKRF
                     DH"
BASE COUNT          874 a         1546 c         1512 g          871 t
ORIGIN      
        1 catcgacggt tccccgcatg agaagcctgc cctgtgccat caccacgacc ccgtcacaca
       61 gccgcgagac gtagtcgagg tcatgctcga tcaggcagaa ggtgaggcca cgctcccggt
      121 tcagcaactt gatcttttcg gcgatcttga ccagcagcgt acggttgata cccgcaccga
      181 tctcgtccag caggatgatg cgcggattgc gcatcaatgc gcggccgagc tcaagcagtt
      241 tcttctggcc acccgaaaga ttgccggcct tctcgtcgat gacccggtcg agttcgagga
      301 acgtgacggt ctcccgggcc gcgcggtaga cggcctcctc ctcgccgcgg aagcgactgc
      361 gccggaacat cacattgaac acattctcgc ccagcggcat ggccgcggct gccatcaggt
      421 tttcaagcac cgtgaggcgc gagaactcct gggggatctg gaatgtccgg gccagtccct
      481 tctcggcccg ctggtgcggc ttgagccgcg tgatgtcgat ggcttccagc aggatcctgc
      541 ccgattgcac cggcacgtgg cccgcgatgg cgttgaacat ggtggtcttg ccagccccgt
      601 tgggaccgat caggccggtg atgcggcccg tcggtatctc gaagctcacg ccatcgacgg
      661 ccttgaggcc accgaagctg acactgacct tgtcgactgt gatcatgaag gaggtcccgc
      721 cccatcgctg gggcgggaac cggacttact gcatcaccgc agtctgcttg tacttgtcgc
      781 catcgaccac gaacttgccg atgtagcctg tgacatcgcc tgcggcatcg aagtcgtagg
      841 caccgccggc gccatcatag tcgatgtcct tgccttcctt gatcagggcc ttggcctttg
      901 cccattcgcc ggggccgacc ttctcgccgg gagccgacgc aacctcgcgc agggctgccg
      961 ccatcttggc gcggtcagcg gaacccgcct tctcgatcgc cagaagcgtg aggaaggtcg
     1021 cgtcataggt ctggtcgacg aaggccttgt cggcgccttc cttgaactcc gcattgtaga
     1081 ggtcatgcag cgtcttctgg ttcggattgt ctgccggcga ggtgggcgag gagaagaagg
     1141 aggtggccaa ggcctcggcg ccgacatcct tgatcagcgc ttcgtcgcgc aggccatcgg
     1201 tgccgatgaa ccgcgtgaag aggccgccct cgatcgcctg cttgacgatc ttgccgccgg
     1261 agtcgccggc ataggccacg acgaccagcg cttcgccgcc cgccttggac agggtcgcaa
     1321 gctcggaacg gtaggagtcc ttcttctctt cgtgcttgac ctcggccaca acctcgccac
     1381 cggcggcctt gtaggcatcg atgaaggtct ggccgatccc gacgccatag tcattgttca
     1441 cgaaggtgac ggcaactttc ttgataccct cgtcgagtac catcttggca agcaccgagc
     1501 cctggtagtt gtctgacggc acgatacggt agagcaggtc attgtccttg acgccagtca
     1561 tggccggcga ggtggcggtc ggcgaaatct ggacgacacc cgccggaatg gcagccgctt
     1621 cggctgctgc gatggtggtc cccgacatga gcgcgcccat gatgatgggc acgttctcga
     1681 tattgatcag cttgccggcg gcgtcgacgg cgccctgtga cgagcccgtc gtgtcgccga
     1741 agatcgcgat ggcctgccca tcgagcaggc caccctgctc gttgacgtgc ttgacggcaa
     1801 gattggttgc attctgcagt ggtggaatga agttggcgat ggggccggtg atgtccatca
     1861 ggatgccgac cttggtatcg gccgccagcg ccagggcgct ggtcccgaac agagccacgg
     1921 cggcgatcgt cactgtgaat tttttcattg ttttcctccc aggattgaaa agtgtccgat
     1981 gctacttgag cgcccctcgg gctgtcatca ggaatcgctc aaaaaccgat gaaatccact
     2041 caaggcccgt cacacgtcct caggcgggcc aggcagaacc tcggtccgga acgcgcctgg
     2101 caaggtcact tccagcagtt gcagatcggg cgtgagcgcg ccgtatcgcg tggccatgcc
     2161 aggcggaatg acgaaggcat cgcccgaggc aagccgatag ggatccttgc cctgcccctc
     2221 cagcaccatc cctcccgaca tgacgaaggt gaagaggatg tcgccgtcgt gccgggtcca
     2281 tggcgcagcc tggccggtgg gccgcgccac cacgactgac gcaacccccc gggtgttggc
     2341 gcagatcgtc gtgtcacgcg cctcgaagcc tggaatgcgg aagggcttga aagctccttc
     2401 cttggccagg ctatggacga agcgctgccc gtcccattcg cgctcgggcc gcaggtccgg
     2461 cgtgggcagc gtcatgtcgt ggtcgatctc ggtgatgtgc tcggccggaa ccccgatctc
     2521 gatcacctcg atgccgtcgg ccgcatgcag caccttgtgc cggatggttg gcggctggat
     2581 gaagcagtcg ccggcggtca accgacgcgg gccgccctgg tcctcataca ggacgtcgac
     2641 ccaaccccgg atgcagaaga tcagctggaa gccgaccttg tggaaatgca ccatgtcggg
     2701 caccggcccg tccggcaccc ggatatgcga ggcgatcatc gccccgccca gcctcgaagg
     2761 aacgagatcg cgatactcca tgccggcgcg gccgatgatc cagggcgcct gatcggccag
     2821 acgtcgcacc acgaaagcgt gctgggtcgg cggcaacacc agcggcggat cgagttcctc
     2881 gatctcgatg cgggttccgt tgggcgcagt caagacgcgc tggccatcgg cgaagccatc
     2941 cggatcgtcg gtgagaatgc gcaaggtgcc gggcgcttcg cgggcaccct tctcgatccg
     3001 cagccgcaag ccatgacccg agagcaccgc gatctgcgga ttgtcggccg gatagatcat
     3061 gtccaggcgc atcttcagcg tcttcgtgaa gaagggcaga tcgttgcgca gctcctgcgt
     3121 cggcagccgg atctcggcgc gggtttcagt catgcgcatg tttcctcgga ctcataccga
     3181 cactttttcg tatttcggct gctgccatgt tttttcggcc aggattcggc gcaggcgctg
     3241 cacagcggtc cagcaatcga catggcggat gaccagcggc gagacgccga agcggatggc
     3301 atctggtttg cggaaggacg agatcacgcc cgcgtcgatc agcgcctcga cgaccgaacc
     3361 ggcaccgggc gaagaaaacg atacgtttcc gccgcgccgc gcatgatcgt ccggcgaatt
     3421 cacggtcacg cccagcgaac cgcattcctg cctgagcgct gagatgagaa agtcggtcag
     3481 cgaccggtgc ttggcccaga gctttgccgg ttcgaccttc ggccagacat cgagaatggc
     3541 cgatgccagt tcatcggcgc cgaccagcgg cgtgcccgtc aggaacttct tcacgccaga
     3601 atgcggcgcg aagtcgcgcg cgaaggcgaa gacatcggca tgccccatcc agccgcaaag
     3661 cgtgggccag acatcgtcgc caagcccggg cttcagatag atgtaggccg gcccccctgg
     3721 cccggtgcag agatacttgt agccacagcc gatggcgagg tcggcatccg cggccccgac
     3781 gtccaccggc acggcaccgg cggagtgcga aagatcccac acggccaacg cgccatggcg
     3841 atgcgccgcc gccgtcacct tcgccatgtc ccagcgctct cctgagcgat agtcgacatg
     3901 gctcagcgcc gcgacggcaa tgccgtcatc gaacgcggca attgcctcgt cctcgctatc
     3961 cacgaaccgc aatgtcacac ggccgcccga ggctttcgca atgccctgca gcacatgcac
     4021 gtcggtcggg aagtttccgt tctgggtgag gatgaccttg cgatctggcc gcagcgccag
     4081 cgcatgggtc acggccttga actggttgag ggtggtggag tcactcatca ccaccgtgcc
     4141 gggcgctgcg ccgatcagcg gcgcaatgca gtcgcccagc agcgagggca tctccagcca
     4201 ttgccgctgg ctccagccgc ggcggcgcag atggacccag tcgtccagca ggcccctggc
     4261 cttctcgcgc acagctgtcg gcatggggcc gaccgaattg gcatccatgt agatgttgtc
     4321 gtcgggacat tcgaagagcg cgcgaatggg cgcgagatcg tccttggcat caagggcttg
     4381 cgccagttcg agggaggtgg atgtcatggc agggagctaa ccacggcacc cgttttcttg
     4441 caatagtgaa agaaacttgc aaatttgcaa gacacctcca taatcgctga catggcaaag
     4501 cagatcaagc tcgccgccga catcggcggc accttcaccg atatcgtgct cgaatccggc
     4561 gccaagcgct ggagcggcaa ggttctcacg acgacggcgg cgccggaact tggcgtgatc
     4621 gagggcatcc ggctggtgct cgaacaatcc gggctgaagc ctgccgacgt tggcgtcttc
     4681 atccacggga cgacgctggc caccaatgcg ctgatcgagc gcaagggtgc caggacagcc
     4741 ttcgtcacca ccgaaggctt ccgcgacatt ctcgaacagg gctacgagaa gcgcttcgac
     4801 cat
//
LOCUS       DBNGVR010000049         4324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1982255, whole genome shotgun sequence.
ACCESSION   DBNGVR010000049 DBNGVR010000000
VERSION     DBNGVR010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1982255"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="AC16A0_01015"
     CDS             complement(<1..862)
                     /locus_tag="AC16A0_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3103935.1"
                     /translation="MNRKFLTLAAGALMLGMSSMSAQAAGKNICVSWKTFQEERWKTD
                     EAAIKAVVEAAGNKYASADAQGSAAKQLTDIEGLITQGCNVILVVAFDSDAILPAFQA
                     AADAGVKMISYDVLVEHPDALYVTFDNVGVGRLMAKTMLEKGKSEGDFAFIKGDKGDP
                     NATFLFQGIMEVLKPNMDAGKIKNVCETFTDGWKPDAAQKNMEQCLTSVNNKVDAVIS
                     QNDGMAGGVVAALEAQGLAGSVPVTGQDGDKAALNRVAIGTQLVSVWKDSRVLGQKAA
                     EFANMIADGKS"
     gene            1088..2335
                     /locus_tag="AC16A0_01020"
     CDS             1088..2335
                     /locus_tag="AC16A0_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3103936.1"
                     /translation="MREKADSELVRRQNRRIVLETLRGQGPQARIGLGRSTGLSPASI
                     TSISSQLIDDGIIVERHDAEILPEPNRRGRPQTLLDLDPGAAQVLAVKISIDGIELAL
                     ADYRGTILHRGSSRLATYDADAAAFGGRVADEISAFLAEAPSPRGRLARIAIAIQGVA
                     DSRAGTIMWSPAFRARNIPVVEPVQKALGIACSISNDANMIAEGLDQRSRGETSAVVF
                     MGYGVGMGLILDGKVYHGPTGAAAEFGHMNHLPGGALCRCGRRGCVEAYAADYGILRS
                     AGTDDDSTPPPLSGVPVETMRKLESQARAGEPRAMEAYRKAGQAVGYGLARLVALLNP
                     TRIVLSGPGTHAIELIRPHLMQAVEDGVVAELRQNLAIDTVPIDTDMIIKGTIDSALR
                     ELDREVFSGGPVGKEAFALQQAV"
     gene            2439..3689
                     /locus_tag="AC16A0_01025"
     CDS             2439..3689
                     /locus_tag="AC16A0_01025"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR3103937.1"
                     /translation="MAAEKPRPPEAVLRTWYKLVLELVRHTATYSPPVASRAFAYLGV
                     TAFEAAASGNPKLRSLQGQLNGLDPLPTRATGVPYDDGVVINAAMRTAVESFFSNTGP
                     TGQRAMGALGSKLSAEVAANLPQEVVARSEAHGKAIADHVIAWSASDGGAVIENMGFP
                     YEYTLTAGPSRWVPTSPIKQQQLPLLPGWGNNRTFAMRDGAACPLPPPPAYSEDVASA
                     FYKEAFEVYETVKTLTPEQRAIARFWSDDPMLSPTPPGHWLVIALSVLEAENADLERS
                     ADLLARIGVTLADAFIACWRSKFEFDLLRPITYIRRVIDPKWEAILITPPFPEYPSGH
                     STQSGAAATVLTAFFGENFAFVDTTHERDGLKPRSFSSFWAAADEAGLSRLYGGIHFR
                     AAIERGLEQGRCVGAASNALQTRV"
     gene            3693..>4324
                     /locus_tag="AC16A0_01030"
     CDS             3693..>4324
                     /locus_tag="AC16A0_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3103938.1"
                     /translation="MTRKACLSVLPLVAAIAASPAALGNEVAMPRFAEETAKAGIESV
                     YTGDWEYMVGGGAAAFDCNGDGFDDLFLAGGASPAKFFVNRSKAGGELTFVPEASGLE
                     RDAVTGAYPLDIDNDGLMDLVLLRVGENIAMRGLGGCRFEPANAAWGFDGGDGWSTAL
                     AATWEKGADWPTIAIGNYIDRKEEISPWGSCTENWLHRPKSGGGFAAPAAL"
BASE COUNT          734 a         1395 c         1408 g          787 t
ORIGIN      
        1 gcgacttgcc gtccgcgatc atgttggcga actcagcggc cttctggcca agaacgcggg
       61 agtccttcca caccgaaacg agctgggtgc cgatggccac gcggttgagg gcggccttgt
      121 cgccgtcctg gcctgtgacc ggcaccgaac ccgcgagacc ctgggcctcg agagcagcaa
      181 ccacgccgcc agccatgccg tcgttctgcg aaatgacagc gtcgaccttg ttgttcaccg
      241 aggtcaggca ctgctccatg ttcttctggg ccgcgtcggg cttccagccg tccgtgaagg
      301 tttcgcagac gttcttgatc ttgccggcat ccatgttggg cttcagcact tccatgatgc
      361 cctggaacag gaaggtggcg ttcggatcgc ccttgtcgcc cttgatgaag gcgaagtcgc
      421 cctcggactt gcccttctca agcatggtct tggccatcag gcggccaacg cccacgttgt
      481 cgaaggtcac gtagagcgcg tcggggtgct cgacgagaac atcgtaggag atcatcttca
      541 cgccggcatc ggcggcggcc tggaaggccg ggagaatggc gtcggagtcg aaggcgacga
      601 cgagaatgac attgcagccc tgggtgatca ggccttcgat gtcggtcagc tgcttggcgg
      661 cagagccctg ggcgtcggcc gaggcatact tgttgccggc ggcttcgacc acggccttga
      721 tggccgcctc gtcggtcttc cagcgctctt cctggaaggt cttccaggat acgcagatat
      781 tcttgccggc ggcctgcgcc gacatcgacg acatgccgag catcagagcg ccggctgcca
      841 gtgtcagaaa cttacggttc attcttgggt cctcctcata gcctcgtggc ccccaaggga
      901 cgcctccctg aaccggttcg tatcaccggg cgcatggggt tcctgggcca tggacgtttg
      961 ctgtttgttt tatttcggcg tccaaaaaaa tgttgcatca aactccgatc ctagtcaaca
     1021 taagaaataa gcaagataaa aatagtgcaa cgcaacatca gttagcagtt gcacaacgag
     1081 tgggagcatg cgcgagaagg ccgacagcga attggtgcgg cggcagaacc gccggatcgt
     1141 tctggagacc ctgcgggggc agggccccca ggcgcggatc ggtcttggcc ggtcgaccgg
     1201 actgtctcct gcttccatca cgtcgatatc gagccaactg atcgacgacg ggatcatcgt
     1261 cgaacggcac gatgccgaga tcctgcccga gccgaaccgc cgcggccggc cgcagacctt
     1321 gctggacctc gatcccggtg ctgcgcaagt gcttgccgtc aagatctcca tcgacggcat
     1381 cgagctcgcg cttgccgact atcgcggcac gatcctgcat cgcggatcat cgcggcttgc
     1441 cacctatgac gccgatgccg ccgccttcgg cggccgcgtg gcggacgaga tatccgcttt
     1501 tctggccgaa gcgccatctc cgcgcggccg gctggcgcgg atcgccatcg ccatccaggg
     1561 cgtggccgat tctcgcgcag gcaccatcat gtggagcccg gccttccgtg cacggaacat
     1621 ccctgtcgtg gagccggtgc agaaggccct gggcattgcc tgcagcatct ccaacgacgc
     1681 caacatgatc gcggaggggc tggatcagcg gtcgcgcgga gagaccagtg ccgtggtctt
     1741 catgggctat ggcgtcggga tgggcctcat cctggatggc aaggtctatc atggcccgac
     1801 cggcgccgct gccgagttcg gccacatgaa ccatctgccg ggcggcgcgc tgtgccgctg
     1861 tggccggcgc ggctgcgtcg aggcctatgc tgcggactat ggcattcttc gaagtgccgg
     1921 cacggatgat gattcaacgc caccgccgct ctcgggcgtg ccggtcgaga ccatgcggaa
     1981 gctggaaagc caggcccgcg ccggcgaacc gcgggccatg gaggcctacc gcaaggcagg
     2041 ccaggccgtg ggctatggtc ttgcccgcct cgtggcgctc ctcaacccga cccgcattgt
     2101 gctgtccggc cccggcacgc acgccatcga actgatccgg ccgcacctga tgcaggcggt
     2161 cgaggacggc gtagtggcgg agctccggca gaatctggca atcgatacag tgcccatcga
     2221 taccgatatg atcatcaagg gaaccatcga cagcgccctg cgcgaactgg atcgcgaggt
     2281 gttttccggt gggccggtcg gcaaggaagc ctttgcactg cagcaggcgg tgtgatggtg
     2341 ttggctctgg agacgaagga gtgctggtga tgaaggtgtc gcggaggctt gcacttgggc
     2401 tcggtctggc tgccttggca agcagtggcc acgttgcaat ggcggctgag aaaccacgtc
     2461 cgcctgaagc cgtgctgcgc acctggtaca agctggtatt ggaactggtg cggcataccg
     2521 ccacctattc gccacccgtt gccagcaggg ctttcgccta tctgggcgtg acggccttcg
     2581 aggccgcggc gagcggcaat ccgaagctgc ggtcgctgca aggccagctg aacggactcg
     2641 atcccttgcc cacgcgcgcg acgggcgttc cctacgatga cggcgtcgtc atcaatgcgg
     2701 caatgcggac ggccgtggaa agcttcttca gcaacacggg gccgaccgga cagcgcgcca
     2761 tgggtgcctt gggttccaag ctttcggccg aggtcgcggc caatctcccg caggaggtgg
     2821 tggcgcgcag cgaggcgcat ggcaaggcca ttgccgatca cgtcatcgcc tggtcggcca
     2881 gcgacggcgg cgccgtgatc gagaacatgg gatttcctta cgagtacacc ctcactgcag
     2941 gccccagtcg gtgggtgccg accagtccga tcaagcagca gcagcttccg cttcttcccg
     3001 gctggggcaa caatcggacc ttcgccatgc gcgatggcgc ggcttgcccg ctgccgccac
     3061 cgcctgccta tagcgaggac gtcgcctcgg ccttctacaa ggaggccttc gaggtctatg
     3121 agacggtgaa aaccctgacg cccgagcagc gcgccattgc gcgcttctgg tcggacgatc
     3181 ccatgctgtc cccgacgccg cccggtcatt ggctcgtcat cgcgctgtcg gtgctggagg
     3241 cggagaatgc ggatctcgag cgttccgcgg atctgctggc acggatcggc gtgaccctcg
     3301 ccgatgcctt catcgcctgc tggaggagca agttcgaatt cgatctgctg cgccccatca
     3361 cctacatccg ccgggtgatc gatcccaagt gggaggcgat cctcatcacg ccgcctttcc
     3421 ccgagtatcc cagcggccac agcacgcagt ctggcgcggc ggccacggtc ctgacggcct
     3481 tcttcgggga gaatttcgca ttcgtcgaca cgacgcatga gcgcgacggc ctgaagccgc
     3541 gctcgttctc cagcttctgg gccgctgccg acgaagccgg cctctcgcgg ctttacggtg
     3601 gcatccattt ccgcgccgcg atcgaacgcg gccttgaaca gggccgctgc gtgggcgctg
     3661 ccagcaacgc cctgcagacg agggtctgag tcatgacacg gaaagcctgc ctgtccgttc
     3721 tgcctctcgt ggcggcgatc gccgcatcgc ccgcagcttt gggcaacgag gtcgccatgc
     3781 cgcgcttcgc tgaggaaact gccaaggccg gcatcgaatc ggtttacacg ggcgactggg
     3841 agtacatggt cggcggcggc gctgccgcct tcgactgcaa cggcgacggc ttcgacgatc
     3901 tcttcctcgc cggcggcgcg tctcccgcga agttcttcgt gaacagaagc aaggcaggcg
     3961 gtgaactcac cttcgtgccg gaagcgagcg gtctcgagcg cgacgcggta accggcgcct
     4021 atcccctcga catcgacaat gacggcctca tggatctcgt gctgctgcgc gtgggcgaga
     4081 acatcgccat gcgcggcctg ggcggctgcc gcttcgagcc cgcgaacgcg gcctggggct
     4141 tcgatggcgg tgacggctgg tcgacggcgc tcgcggccac gtgggagaag ggcgccgact
     4201 ggcccaccat cgccatcggc aattacatcg accggaagga agagatatcg ccctggggct
     4261 cctgcaccga aaactggctg caccggccga aatccggcgg tggatttgca gctccggcgg
     4321 cgct
//
LOCUS       DBNGVR010000050         3286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1588993, whole genome shotgun sequence.
ACCESSION   DBNGVR010000050 DBNGVR010000000
VERSION     DBNGVR010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1588993"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="AC16A0_01035"
     CDS             complement(<1..536)
                     /locus_tag="AC16A0_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HIR3103939.1"
                     /translation="MTDAAERKLEADPSEVVATGLAEAMVIDPEEQLATRAAWLYFVA
                     GLTQIEIGRKLGVNRIRVNRLLAQARRRGLVQIRISGRLANCVELEERLKDRFKLEEA
                     IVVPTPPGQALVPHVIATAAGQALASRLKDGMSVGVGWGRTLRLSLRTVPRMSMPNLS
                     VVSLLGGLTRSSAMNPHET"
     gene            complement(575..1633)
                     /locus_tag="AC16A0_01040"
     CDS             complement(575..1633)
                     /locus_tag="AC16A0_01040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3103940.1"
                     /translation="MSTVTILGAGVMGSSMSLPACDRGHTVRLVGTHLDRDIIDSVAD
                     TGRHPKLNVKLPDGISAFHHEDLAGAFGTDTDLIVLGVSSAGIGWAIDRICDALVQPV
                     PIVMITKGMHPEAAGLTALPDHVAREVKRRKGFDLTVAAIGGPCIAGELAVRRHTGTV
                     ITSRDGGFARSLCAMLETDYYHPRPSDDVVGVEVCAAFKNFFAIAVGWAAGHLEQLPP
                     TENRAFNHNAAAIIFDQAVRELMELTRALGGADASVWGMPGAGDLYVTCQAGRNSRLG
                     NNLGRGLTYSQTKAGPMKGDTIEGAELGVTVAPALRAMMSSGALKPDRMPLTRALLDA
                     LTGDRPLELPWAAFHRQA"
     gene            complement(1630..2685)
                     /locus_tag="AC16A0_01045"
     CDS             complement(1630..2685)
                     /locus_tag="AC16A0_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3103941.1"
                     /translation="MASVNVKKVFKKYGDVEVVHGIDLEIEDGSFVVLLGPSGCGKST
                     LLRMIAGLESITSGTVEIAGRRVNDVHPKDRNIAMVFQNYALYAHMKVRDNMSFSLQL
                     AKKPQTEIDEKVAWAAKILNLEPYLDRYPRELSGGQRQRVAMGRAIVRNPAVFLFDEP
                     LSNLDAKLRVQMRTEIKELHERLKTTTVYVTHDQIEAMTMADKIVILRDGVIEQVGTP
                     LEVYDHPTNLFVAEFIGSPAMNLLRGAVVKDGVKVADGTVLPLPAKHKARAGQTVVYG
                     IRPEHLKPSKKGSKAKVVVVEPTGPEIHIYADLGGQEVCAITDERLVLARDDMIDLSP
                     KLDKIHLFDAESGKALA"
     gene            complement(2701..>3286)
                     /locus_tag="AC16A0_01050"
     CDS             complement(2701..>3286)
                     /locus_tag="AC16A0_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HIR3103942.1"
                     /translation="LHRVLTLCGLGVCFTGVSHHGSMGEHQVSHWIDMFAGEKHPGST
                     HGQQVGVASLAIARLHHEILALDQPPLIKATHIVESEFIQRYGQDIGRMCYAEAKKKS
                     FDRTGAEAFNRKLKDLWPQLREELRPMLMDPSAMKATLAAAGGPTTATELGLSRKVWR
                     DAMKHARDVRNRWSFLDLADDAGLLDDFLDRDEQ"
BASE COUNT          559 a         1081 c         1053 g          593 t
ORIGIN      
        1 gtctcgtgcg ggttcatcgc cgaactgcgc gtcaggccgc ccagcagcga gaccaccgag
       61 aggtttggca tcgacatgcg cggcacggtg cgcagcgaca ggcgcagtgt ccttccccag
      121 ccgacgccaa ccgacatgcc gtccttgagg cggctggcca gcgcctggcc cgcggcggtc
      181 gcgatcacat gcggcaccag cgcctggccc ggcggcgtgg gcacaacgat ggcctcttcc
      241 agcttgaagc ggtccttgag cctctcctcg agctccacgc agttggcgag ccgtcccgaa
      301 atgcgaatct gcaccagtcc acgccgccgc gcctgcgcca gaagccggtt cacccggatg
      361 cggttcacgc caagcttgcg gccgatctcg atctgggtca gcccggcgac gaaatagagc
      421 caggccgcgc gcgtggccag ctgctcctcg ggatcgatca ccatggcctc ggcaagtccg
      481 gtggcgacca cttcggacgg atccgcttcc agcttgcgct ccgccgcgtc tgtcatgccg
      541 cctggcctgc cggacatcgg gacctctctc cccttcacgc ctgacgatgg aacgcggccc
      601 agggaagttc caacggccta tcgccagtca gggcatcgag caatgcccgg gtaagcggca
      661 tgcgatcggg cttcagcgcc ccggacgaca tcatggccct gagcgccggc gccaccgtca
      721 cgcccagttc ggcaccctcg atggtatccc ccttcatcgg gccagccttg gtctggctat
      781 aggtgagccc acggcccaga ttgttgccca gccgcgaatt gcgccctgcc tggcaggtga
      841 catagaggtc tcccgccccc ggcatgcccc agacactggc atcagcgccg cccagggcgc
      901 gcgtcagttc catcagttca cggacggcct gatcgaagat gatggccgcg gcattgtggt
      961 tgaaggccct gttctcggtc ggcggaagct gttcgagatg gcccgcggcc cagcccacgg
     1021 caatggcgaa gaaattcttg aaggccgcgc agacctccac gccgacgacg tcatccgagg
     1081 ggcgcggatg atagtaatcg gtttccaaca tggcacagag cgagcgggcg aagccgccgt
     1141 cgcgcgaggt gatcaccgtg ccggtgtggc gccgcaccgc aagctcgccc gcgatgcaag
     1201 gcccgccgat ggcggcgacc gtcagatcga atcccttgcg ccgcttcacc tcgcgggcga
     1261 catgatcggg aagcgccgtc aggccagccg cttccggatg catgcccttg gtgatcatca
     1321 caattggcac cggctgtacc agcgcgtcgc agatcctgtc gatggcccag ccgatgccgg
     1381 cagagcttac gcccagcacg atcaggtcgg tatcggtgcc aaacgcccct gcaaggtctt
     1441 catgatgaaa ggcactgatg ccatcgggca gcttgacatt gagcttcgga tgccggccgg
     1501 tgtccgcaac actgtcgatg atatcccgat caagatgtgt gccgaccagc cgtacggtgt
     1561 gcccgcgatc gcaggcaggc aggctcatgg aagagcccat gacgcccgca cccaggatgg
     1621 tgaccgtgct cacgccaggg cctttccgct ttccgcatcg aagaggtgga tcttgtcgag
     1681 cttgggcgac agatcgatca tgtcgtcacg cgcgagaaca agccgctcgt ccgtgatggc
     1741 gcagacctcc tgcccgccga gatcggcgta gatgtggatc tccgggccgg tcggctcgac
     1801 caccacgacc ttggccttgc tgcccttctt ggagggcttc agatgctcgg gccggatgcc
     1861 gtagaccacc gtctgccccg ccctggcctt gtgcttggcc ggaagcggca gcacggttcc
     1921 atcggcaacc ttgacgccgt ccttgaccac ggcgccacgc agcaggttca tcgcaggcga
     1981 accgatgaac tcggcaacga agaggttggt gggatggtca tagacctcga gcggcgtgcc
     2041 gacctgctcg atcacgccgt cgcgcaggat gacgatcttg tcggccatgg tcatggcctc
     2101 gatctggtcg tgcgtcacgt agaccgtcgt ggtcttgagc cgctcgtgca gttccttgat
     2161 ctcggtgcgc atctgcacgc gcagcttggc atcgagattg gagagcggtt cgtcaaagag
     2221 gaagaccgcc ggattgcgca cgatggcgcg gcccatggcc acgcgctggc gctggccgcc
     2281 cgagagttcg cgcggatagc ggtcgagata gggttcgagg ttgagaatct tggcggccca
     2341 ggccaccttc tcgtcgatct ccgtctgtgg ctttttcgcc agctgcagcg agaagctcat
     2401 gttgtcgcgc accttcatat gcgcatagag cgcataattc tggaacacca tggcaatgtt
     2461 gcggtccttg ggatgcacgt cgttgacgcg cctgccggcg atctcgaccg tgccggacgt
     2521 gatcgactcg agaccggcga tcatgcgcag caaggtggac ttgccgcagc cggagggtcc
     2581 cagcagaacc acgaagctgc cgtcctcaat ctccaggtcg attccgtgga cgacttcgac
     2641 gtcgccatac ttcttgaaga ccttcttgac gtttaccgat gccatgccgt cacctggttc
     2701 ctactgttca tcgcggtcga gaaaatcgtc gaggaggccg gcgtcatccg caagatccag
     2761 gaaggaccag cggttgcgca cgtcgcgggc gtgtttcatc gcatcgcgcc agaccttgcg
     2821 cgacaggccg agctcggtcg cggtggtcgg gccgccggcg gcggcaagcg tcgccttcat
     2881 ggctgatggg tccatcagca tgggacgaag ctcttcgcgc agttgcggcc agaggtcctt
     2941 gagcttgcga ttgaaggcct cggcgccggt gcggtcgaag gacttcttct tcgcttccgc
     3001 atagcacatg cggccgatgt cctgtccgta gcgctggatg aactccgact cgacgatgtg
     3061 cgtcgccttg atcagcggcg gctggtccag cgccagtatt tcgtgatgca gccgcgcgat
     3121 ggccagcgac gcgacgccga cctgctggcc atgggtggag ccggggtgct tttcaccggc
     3181 gaacatgtcg atccagtgcg acacctggtg ctcgcccatg gagccatggt gcgacacgcc
     3241 ggtgaagcag acgccgaggc cgcacagcgt cagcacgcga tgcagg
//
LOCUS       DBNGVR010000051         4269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3357626, whole genome shotgun sequence.
ACCESSION   DBNGVR010000051 DBNGVR010000000
VERSION     DBNGVR010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3357626"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="AC16A0_01055"
     CDS             complement(<1..592)
                     /locus_tag="AC16A0_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3103943.1"
                     /translation="MSDASHVIDAEDGETLAEREFGFWLFLMSDAIVFALLFATYAVL
                     SQGHGGSHGPAGLFDLTRSGLETGLLLLSSLSFGLAVLALDEDRRWPTIFWLLLTALL
                     GLSFLSLEISDFRDLIARGLAPQENGFLSAVFVLIGTHGLHVAMGILWIIILAVQLMM
                     QGDDPQTRSRLLRLGYYWHFLDIVWIGIYSVVILPGF"
     gene            complement(589..2550)
                     /locus_tag="AC16A0_01060"
     CDS             complement(589..2550)
                     /locus_tag="AC16A0_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR3103944.1"
                     /translation="MLGKLTFDALPFYSGIAFGGALITVLGAAGAMAMITWLGWWRWL
                     WTGWLTSVDHKRVGIMYLVLAGIMLLRGFVDAAMMRAQQAIAVQSDGYLPPGHFDQIF
                     SSHGTIMIFFVAMPFLTGLINFVMPQQIGARDVAFPFINAVSLWLTAAAAALIMASLV
                     LGRFSTAGWTAYPPYSGLDYSPGVGVDYWIWAVMLSGAGTTLTGINFVVTILRKRAPG
                     MRMMYLPLFTWTALCSSILMVFAFPALTVVGALLGLDRYLGMHFFTNDSGGNMMQYAN
                     LIWIWGHPEVYILILPAFGIYSEVVATFSGKRLFGYASLVYATVAITLLSFTVWLHHF
                     FTMGSSASVNAAFGIATMIIAVPTGVKIYDWIFTMYRGRIRMEVPMLWTMGFMVTFVI
                     GGMSGVLLAVPPVDYLMHNTTFLVAHFHNMLIPGALFGYFAGYNFWFPKAFGFRLDDR
                     WGRRAFWAWITGFYLAFMPLYVLGFMGMPRRMEHYDTAAWQPLLIVAACGAALIAAGI
                     FCQIAQLVVSIRNRALLAENAEDPWDGHTLEWSTASPPAPYNFALLPQITGRATHLNA
                     KRTGTAKGPAPAYRAIALPRNTAAGLIIGAMAFAFGFAMVWHIWWLAAVAAAAILAVT
                     VLRVFDRRGPLVLTAEQVAAMEAARAVTA"
     gene            complement(2552..3409)
                     /gene="cyoA"
                     /locus_tag="AC16A0_01065"
     CDS             complement(2552..3409)
                     /gene="cyoA"
                     /locus_tag="AC16A0_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HIR3103945.1"
                     /translation="MAGRLFAGMAAALALTGCTFGSADDFGAAGPVSGTIRELRITAA
                     SVMLIVVIPVYLLTIGFAWRYRASNRSARYAPDWAYSPRIDFVIWAVPTLIIVALGTL
                     VWIYTHRLDPYRPLQGEGPPLVIQAVAMDWKWLFIYPEHKVAAVNELVIPSGRPVTIA
                     LTSDTVMNAFFVPDLAGQIYAMAGMETRLNFRADRDGAYVGRNTQYSGSGFADQSFTL
                     RAVPAQDYDDWIDLLRQSPRALDPEAYAALAAPSSKVPVAYYSAVPEDLFASVIASYR
                     LPSTHAGHE"
     gene            complement(3672..4043)
                     /locus_tag="AC16A0_01070"
     CDS             complement(3672..4043)
                     /locus_tag="AC16A0_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103946.1"
                     /translation="MLDIVKISEQLFAKLQQEADVLMSDRLLSQQVLEARTRELMSKR
                     DDEMNRIEQWAAQQKALISEIFSALIAEVEADRQRNESNLMRMKGEYSFEKSRSEAAP
                     ARDSSNPRVVEQSERFLKAAQ"
BASE COUNT          856 a         1276 c         1451 g          686 t
ORIGIN      
        1 tgaatcccgg aagaatgacc acggaataga tgccgatcca gacgatgtcg aggaagtgcc
       61 agtagtagcc caggcgaagc aggcgggacc gcgtctgggg atcgtcgccc tgcatcatca
      121 gctgtacagc gaggatgatg atccacagga tgcccatggc gacatgcagg ccgtgtgtgc
      181 cgatcaggac gaagacggcc gacaggaagc cgttctcctg cggcgcaagc ccgcgggcga
      241 tgagatcgcg gaaatcgctg atctcgaggg agaggaagct gaggccgagc agggcggtga
      301 ggaggagcca gaagatggtc ggccagcgtc ggtcctcgtc cagggcgagc acggccagac
      361 cgaagctgag gctgctgagc agcaaaaggc ctgtctcgag cccgctgcgg gtgagatcga
      421 agaggccggc tggcccgtga cttccgccat gaccctgcga caataccgca taggtcgcaa
      481 acagcagcgc gaagacgatg gcgtcgctca tcaggaagag ccagaagccg aactcgcgtt
      541 cagccagcgt ctcgccgtcc tccgcgtcga tcacgtggct tgcgtcgctc atgccgtcac
      601 cgctctcgct gcttccattg cggccacctg ttcggccgtc aacaccagcg ggccgcgccg
      661 gtcgaagacg cgcaagacag tgacggcaag aatcgccgcc gccgccacgg cggcgagcca
      721 ccagatgtgc cagaccattg caaagccgaa agcgaaggcc atggcgccga tgatcaggcc
      781 ggccgccgtg ttgcgcggga gcgcgatggc gcgataggcg ggcgctggcc ccttggccgt
      841 tccggtccgc ttggcgttca gatgcgtggc ccggccggta atctgcggga gcagtgcgaa
      901 gttgtaaggt gccggcggcg aggcggtgga ccattccagc gtgtgtccgt cccacggatc
      961 ttccgcattc tcggccagaa gggcgcggtt gcggatgctg accacgagct gcgcgatctg
     1021 gcagaagatg ccggccgcga tcagtgcggc gccgcaggcg gcgacgatga gcagaggctg
     1081 ccaggctgcg gtgtcgtaat gctccatccg gcgcggcatg cccatgaagc cgagaacata
     1141 gagcggcatg aaggcgagat agaagccggt gatccaggcc cagaaggcgc ggcggcccca
     1201 gcggtcgtcg aggcggaagc cgaaggcctt ggggaaccag aaattgtatc cggcgaaata
     1261 gccgaacagg gccccgggga tcagcatgtt gtggaagtgc gccacgagga acgtcgtatt
     1321 gtgcatgaga tagtcgacgg gcggcaccgc cagcaacacg ccggacatgc cgccgatgac
     1381 gaaggtcacc atgaagccca tggtccagag catgggcacc tccatgcgga tccggccgcg
     1441 gtacatggtg aagatccagt cgtagatctt cacgcccgtg ggaaccgcga tgatcatggt
     1501 ggcgatgccg aaagcagcgt tgacgctggc gctgctgccc atggtgaaga agtgatggag
     1561 ccacaccgtg aaggacagca gcgtgatcgc caccgtggca tagaccagcg aggcatagcc
     1621 aaagagccgc ttgcccgaga aggtggcgac cacttcggaa tagatgccga aggccggaag
     1681 gatgaggatg tagacttccg gatgccccca gatccagatg aggttggcgt attgcatcat
     1741 gttgccgccg gaatcgttgg taaagaaatg catgccgaga tagcggtcga ggcccagcaa
     1801 tgcgccgacc actgtcaggg ccggaaaggc gaagaccatg aggatgctgg agcacagcgc
     1861 cgtccaggtg aagaggggca ggtacatcat gcgcatgcca ggcgcgcgct tgcgcagaat
     1921 ggtcacgacg aaattgatgc cggtcagcgt ggtgccggcg ccgctcagca tcacggccca
     1981 gatccagtag tcgacgccca cgccggggct gtagtccaga cccgaatagg gcggataggc
     2041 cgtccagccc gcggtcgaga agcggccaag cacaagcgag gccatgatca gggccgcggc
     2101 cgccgcggtc agccacaggc tgacggcgtt gatgaaggga aaggccacgt cgcgcgcgcc
     2161 gatctgctgc ggcatgacga agttgatcag cccggtgagg aatggcatgg ccacgaagaa
     2221 gatcatgatc gtgccatgcg aggagaagat ctggtcgaag tggccgggcg gcagatagcc
     2281 gtccgattgc acggcgatgg cctgctgggc ccgcatcatc gcggcatcga cgaagccgcg
     2341 cagcagcatg attccggcaa ggacgaggta catgatgccc acgcgcttgt ggtcgacgct
     2401 ggtgagccag cctgtccaga gccaacgcca ccaccccaac catgtaatca tggccatcgc
     2461 gcctgccgcg cccagcacgg tgatcagcgc gccgccgaag gcgatgccgc tgtagaaggg
     2521 cagggcgtcg aaggtcagct tgcccagcat gtcactcatg tccggcatgt gtggagggca
     2581 gccggtagct tgcgatgacc gaggcgaaga gatcctccgg caccgccgag tagtaggcaa
     2641 ccggaacctt gctgctcgga gcggcgagcg cggcataggc ctccgggtcc agcgcacgcg
     2701 gcgactggcg caacaggtcg atccagtcgt catagtcctg tgccggaacc gcacgcagcg
     2761 tgaaggactg atcggcgaag ccggagccgc tgtactgcgt gttgcggccc acataggcgc
     2821 cgtcacggtc ggctcggaag ttgaggcgcg tctccatgcc cgccatggca tagatctgcc
     2881 cggcgagatc cggcacgaag aaggcgttca tcaccgtgtc ggaggtcagc gcgatggtca
     2941 ccggacggcc gctcgggatc accagttcgt tgacggcggc aaccttgtgt tcgggataga
     3001 tgaacagcca cttccagtcc atcgccaccg cctggatgac cagaggcggt ccctcgccct
     3061 gcagcggccg gtagggatcg aggcggtggg tatagatcca gaccagcgtg ccgagcgcga
     3121 cgatgatgag ggtcggcacg gcccagatga caaagtcgat gcgcggcgag taggcccagt
     3181 ccggcgcata tcgcgcagac ctgttggagg cgcggtagcg ccaggcaaag ccgatggtca
     3241 acaggtagac ggggatcacg acgatcagca tgacgcttgc ggccgtgatc cgcagttcgc
     3301 ggatggtgcc gctgaccggg cctgcggcac cgaagtcatc cgcacttccg aatgtacagc
     3361 cggtcagggc aagcgcggca gccataccgg caaacaggcg tccggccatc cttgctgtgc
     3421 cacaatggct ctcgccccgc atcgatctcc cgccagccca cagctcgaac gcaaagtgat
     3481 tcgcaggcta gaggatcgcg gcaggatggt cgagcggatt tggggtggag agggcggctg
     3541 ggcgtggcgg caagcgcctg gctgcggctt caccgctgcc agaaacatcg atccgccgtg
     3601 atgggacggt catgggcccg catcacatag gccggtcgag gggagcgcag cgcgatcccc
     3661 tcgccagaaa cttactgagc ggccttgagg aagcgctcgc tctgctccac cacgcgggga
     3721 ttggaggagt cgcgggccgg cgcggcttcg ctgcgcgact tctcgaagct gtattcgccc
     3781 ttcatgcgca tcaggttcga ctcgttgcgc tggcggtcgg cctccacctc ggcgatcagg
     3841 gccgagaaga tctcgctgat cagcgccttc tgctgcgcgg cccactgctc gatgcgattc
     3901 atctcgtcgt cgcgcttgct catcagctcg cgggtgcggg cctccaggac ctgctggctc
     3961 agcagccggt ccgacatgag cacatcggct tcctgctgaa gcttggcaaa aagctgttca
     4021 ctgatcttga cgatatccaa catttcctgt gctctctctt tttggtggtc ctgactcttc
     4081 gtacaggtaa tttatcgatg agagataaat tgcacagtat acggattacc gcgagcttta
     4141 ccgccacagg tagtaaatcg ttagggttaa cgccggcctc gcggcttccc gagagtcagg
     4201 atttcttcgg gtcgctgagc ttgagcttcg ttttgtggtc gtccaccgga caggctccgc
     4261 cgctgggca
//
LOCUS       DBNGVR010000052         2224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2572247, whole genome shotgun sequence.
ACCESSION   DBNGVR010000052 DBNGVR010000000
VERSION     DBNGVR010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2572247"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="AC16A0_01075"
     CDS             <1..906
                     /locus_tag="AC16A0_01075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3103947.1"
                     /translation="HGPVKVLRWLSKPLAQYGDVLMAPGLCPRAWLPAALRLIRDESG
                     ADMIRLRHVRADAAIAPFLTTHFRDSQQHDLAPVLDLTAFANEAAYEARYTSSQRKRR
                     KKIRKGLEDAIGPVQFEILDQGSACDQAIAEAVHEKHRWLAARGRKNQALCEDKIAAF
                     LRELSRRRAGSVSLVTSCMTAGGKPVSWEIGLRKGRTHFGFITSHRTDLTDYSPGRLH
                     MDLSQRQVLRDGMSAFDLMVPNDPHKESWSSSKVEVRDHHLPLTALGRLYSLGYVETA
                     RPLLRKAYYAMPPELLKLLKPVTGH"
     gene            1042..2196
                     /locus_tag="AC16A0_01080"
     CDS             1042..2196
                     /locus_tag="AC16A0_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3103948.1"
                     /translation="MAVANAFAFSTWNALHTNYAVDVIGFGGEQQGILHTLREIPGFL
                     AFAAVFLLLVMREQTLGILSVLTIGVGVAITGFFPSALGFYATTVVKSLGFHYYETVA
                     QSLALQWFDKKTAPSRLGRIYSLASMSTIAAYGLLFVTWKWFGLDYVWAFLGGGLVAI
                     AIGLFVWLTFPRFENGVIQRKHLVLRRRYWLYYALTFISGARRQIFIIFAVLMMVQKF
                     GYSVSDMTMLLLGNALFTTVIAPRLGRFIEIWGERRSTIIEHVGLMIVFAAYALVADP
                     WIAAMLYMLDNAFFSMSIAHRTYFQKIGDPEDMAPTAGVAFSINHIAAVFIPIPLGLL
                     WDVHYTWVFWIGTGIAALGLAISFLVPRDPARGNEVAWPAAALRTATETP"
BASE COUNT          367 a          754 c          648 g          455 t
ORIGIN      
        1 catgggccgg tcaaggtcct gcgctggctg tccaagcctc tggcgcaata cggcgacgtg
       61 ttgatggccc ccggtctctg cccgcgggcc tggctgccgg cagccctccg cttgatccgg
      121 gacgaaagcg gtgccgacat gatccgcctg cgtcacgtcc gggccgatgc cgcaattgca
      181 cctttcctaa cgacgcattt ccgcgacagc cagcagcacg atctggctcc ggttctcgac
      241 ctgacggcct tcgccaacga agccgcctat gaagcccgct atacatcgag ccagcgcaaa
      301 cgccggaaga agatccgaaa gggcctggaa gacgccatcg gaccagtaca gttcgagatc
      361 ctggatcagg gatcggcgtg cgatcaagcc atcgccgagg cggtgcacga aaagcaccga
      421 tggctggcgg cacgcgggcg aaagaaccag gccctctgcg aagacaagat cgcggccttt
      481 ctcagggagc tttcgcgccg ccgcgcgggt tcggtcagtc ttgttaccag ctgcatgacc
      541 gcaggcggca agcccgtgtc ctgggaaatc ggcctgcgca agggacgcac ccacttcggt
      601 ttcatcacgt ctcaccggac tgacttgacc gactactcgc cggggcggct gcacatggac
      661 ctgtcacagc gccaggtcct gcgcgacggc atgtccgcat tcgacctcat ggtgccgaac
      721 gaccctcaca aggagtcctg gagctcatcg aaggtcgagg tccgggatca ccacctgccg
      781 ctgacggccc ttggccggct ctacagtctc ggctacgtcg agacggcccg gcccctgctg
      841 cgcaaggcct attatgcaat gccgcccgaa ctgctgaagc ttctgaagcc cgtgaccggg
      901 cattgacgcc ctgcgacgga tgcatgctgt ttgcccgtgt gccgccgctg gggctcctct
      961 atagtccgcc ggatggcacc tgacccgacc cctgcagaag gcgcccgcgg ctggcgaacg
     1021 ccctttgcgg cgctggctct catggccgtt gcgaatgcct ttgcgttctc cacctggaac
     1081 gcgctgcata cgaattatgc cgtcgacgtc atcggcttcg gcggcgaaca gcagggcatc
     1141 cttcataccc tgcgcgagat tccgggcttc cttgcctttg ccgcagtgtt cctgctgctc
     1201 gtcatgcgcg agcagactct cgggattctc tccgtcctga ccatcggcgt aggcgtggcc
     1261 atcaccggct tctttccctc ggccctcggc ttctatgcga ccaccgtcgt gaagtcgctc
     1321 ggcttccact actacgagac agttgcccag tcgctggcgc tgcaatggtt cgacaagaag
     1381 acggcaccca gtcgccttgg ccggatttac tcccttgcct cgatgtcgac cattgccgcc
     1441 tacggactgc tcttcgtcac ctggaaatgg ttcggcctgg actatgtctg ggccttcctg
     1501 gggggcggcc tggtcgccat tgccatcgga ctcttcgtct ggctcacctt cccgcgcttc
     1561 gagaatggcg tgatccagcg caagcatctg gtattgcgca ggcgctattg gctctactat
     1621 gccctcacct tcatcagcgg cgcacggcgg cagatcttca tcattttcgc cgtgctgatg
     1681 atggtccaga agttcggcta cagcgtttcc gacatgacca tgctgttgct cggcaatgcg
     1741 ctcttcacca cggtgatcgc gccccgcctt ggccgcttca tcgagatctg gggcgagcga
     1801 cgttcgacca tcatcgagca tgtggggttg atgatcgtct tcgcggccta tgccctcgta
     1861 gccgacccgt ggatcgccgc catgctctac atgctcgaca acgccttctt ctcgatgtcg
     1921 attgcgcacc gcacctactt ccagaagatc ggcgaccccg aggacatggc gccgacggcg
     1981 ggcgttgcct tctcgatcaa tcacatcgca gccgtgttca ttcccattcc gctgggcctg
     2041 ctctgggacg tccactacac atgggtcttc tggatcggca cagggatcgc ggcgctgggc
     2101 ctcgccatct ccttcctggt gccgcgcgac ccggccagag gcaatgaagt ggcctggccc
     2161 gctgctgccc tgaggactgc caccgaaacg ccttgagttg ttcacaatga ccactgtgat
     2221 gcgc
//
LOCUS       DBNGVR010000053         3269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3161051, whole genome shotgun sequence.
ACCESSION   DBNGVR010000053 DBNGVR010000000
VERSION     DBNGVR010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3161051"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /gene="hutH"
                     /locus_tag="AC16A0_01085"
     CDS             complement(<1..1238)
                     /gene="hutH"
                     /locus_tag="AC16A0_01085"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR3103949.1"
                     /translation="MIWIGDRQLTLDDARAALDGPIAVTLTEAAEGKIRRSAETVQRL
                     LATGDAIYGVNTGFGKLAKTRIAAADLSQLQINIVRSHAAGVGAPLEAGVVRLILLLK
                     LNALAQGASGISLDAMAALAALINHDVLPVIPGQGSVGASGDLAPLAHMSLLLIGEGA
                     ATVKGKRLNGADALAAAGTKAVVLGPKEGLALLNGTQVSTALALAGLMAAERNAAAAI
                     LAGALSVDAVMGSDTPFDPRIHALRPHPGQQLAATHYRRLLAGSQIRASHLTDDDRVQ
                     DPYSFRCQPQVMGACLDLLAQAARMLTIEANAVSDNPLVYAEDGAVLSGGNVHAEPVA
                     FAADMTAMAIAEIGSLSERRIAALIDSSISLLPPFLIRNPGLNSGFMIPQCTAAALMS
                     ENKQMSHPASVDSAPTSANQ"
     gene            complement(1235..2599)
                     /locus_tag="AC16A0_01090"
     CDS             complement(1235..2599)
                     /locus_tag="AC16A0_01090"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HIR3103950.1"
                     /translation="MKSAKFRFPSALTANGWQEDVEVAVDAGGMIAAVTPGGKEGQQV
                     SGIAVPAVPNVHSHAHQRFMLGLAEKAGPGADSFWTWREVMYGFALKLGPADLEAVAA
                     QLYVEMLKSGFSVVGEFQYLHHQPDGVPYANPAELSLRCLAAAETAGIAITLLPTLYN
                     HGGFGGQPAIAGQRRFVNDANRYLKIQETLRGAVTGHPLRRLGLSPHSLRAVTAELLN
                     EVLAGIDTVAPVHIHVAEQTKEVDDCLAWCGKRPVDLMLDSFDVSPRWTAIHATHMTV
                     EETARLARSGAIAGLCPTTEANLGDGIFPAVDYMKAGGAIAIGSDSHISVSPAEDIRM
                     LEYSQRLRDRTRNALAGGPGQSTGRTLFDAVCKGGAASMAQPVGAIAPGRRADITVLD
                     ADHPTLIGRSRDDALDGWIFSGGNPCVKDVIAGGIHVVKDRVHIREEPVAKAFRAAVK
                     RLTA"
     gene            2647..>3269
                     /locus_tag="AC16A0_01095"
     CDS             2647..>3269
                     /locus_tag="AC16A0_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase-like domain-containing
                     protein"
                     /protein_id="HIR3103951.1"
                     /translation="MLLTNLQAATMTGGYGIVAKAAVLIENGRIAWVGPAAEAPSGSA
                     IDCGGRLLTPGLVDCHTHLVYGGNRANEFELRLTGVPYAEIAKAGGGIAATVRATRTE
                     SEAPLLASALKRLDTLLAEGITTIEVKSGYGLDIDTELKMLRVARQLGQDRPVDVRTT
                     FLGAHTFPPDYRDRRADYVRLVCDQALPAAAKAGLADAVDAFCESLA"
BASE COUNT          544 a         1062 c         1132 g          531 t
ORIGIN      
        1 tcctggttgg ccgaggtcgg cgccgaatcc accgaggccg gatgcgacat ctgcttgttc
       61 tcgctcatca gcgccgcggc cgtgcattgc gggatcatga agcccgagtt cagccccgga
      121 ttgcggatca ggaagggcgg cagcagcgag atggaggaat cgatcagcgc cgcgatgcgc
      181 cgctcggaga gcgagccgat ctcggcgatg gccatggccg tcatgtcggc cgcgaaggcc
      241 acgggctcgg cgtggacgtt gccgcccgac agcaccgcgc cgtcctccgc gtagacgagc
      301 ggattgtcgg acacagcatt ggcctcgatg gtaagcatgc gggccgcctg ggcaagcagg
      361 tcgagacagg cgcccatgac ctgcggctgg caacggaagg aatagggatc ctgtacccgg
      421 tcgtcatcgg tcagatgcga ggcgcggatc tggctgccgg caagaagcct gcggtaatgc
      481 gtggcggcca gctgctggcc gggatgcggc ctgagcgcat gaatgcgcgg atcgaagggc
      541 gtgtcggagc ccatcacggc gtcgaccgag agcgcgccgg ccagaatggc ggcagcggca
      601 ttgcgttccg cggccatgag gcccgccagc gccagggccg tcgagacctg ggtaccgttg
      661 agcagcgcca ggccttcctt tgggccgagg acgacggctt tggtccccgc ggcagccagc
      721 gcatcggccc cgttcaggcg cttgcccttc acggtcgcag cgccttcgcc gatgagcagg
      781 aggctcatgt gggccaaggg cgccagatcg cccgaagcgc cgaccgatcc ctgtccggga
      841 atgaccggga gcacgtcatg attgatgagg gcggccagcg cggccatggc gtcgagcgag
      901 atgccggagg ccccctgggc cagcgcattg agcttgagca ggagaatgag gcgcaccacg
      961 cccgcctcca gcggcgcgcc gacgcccgcg gcatgcgagc gcacgatgtt gatctgcagc
     1021 tgcgaaagat cggcggcggc gattcgggtc ttggccagct ttccgaagcc cgtgttgacg
     1081 ccatagatcg cgtcgcccgt ggcgagcagg cgctgcacag tctctgcgct gcgccggatc
     1141 ttgccctccg cggcctccgt gagggtcacg gcgatggggc cgtcaagtgc tgcgcgcgcg
     1201 tcgtcgagag tgagctggcg gtctccgatc cagatcatgc tgtgagcctc tttacggcgg
     1261 cgcggaacgc tttcgcgacc ggttcctcgc ggatgtgcac gcggtccttc accacatgga
     1321 tgccgccggc gatgacgtcc ttcacgcaag gattgccgcc cgagaagatc cagccgtcca
     1381 gcgcgtcgtc gcgggatctc ccaatgaggg ttgggtggtc ggcgtctagc accgtgatgt
     1441 cggcgcggcg gccgggtgcg atcgcgccca ccggctgcgc catggaggcg gcaccgccct
     1501 tgcagaccgc atcgaacagc gtgcgcccgg tcgactggcc gggaccgccc gccagcgcgt
     1561 tgcgggtgcg gtcgcgcagg cgttgcgagt attccagcat acggatgtcc tcggcgggcg
     1621 aaacggagat gtggctgtcg gatccgatgg cgatggcgcc gccggccttc atgtagtcca
     1681 ccgccgggaa gatgccgtcg cccagattgg cctcggtggt ggggcagagc ccggcgatgg
     1741 cacccgaacg cgccagccgc gcggtctctt ccaccgtcat gtgagtggca tgaatggcag
     1801 tccagcgcgg tgagacgtcg aaactgtcga gcatcaggtc gaccggccgc ttgccgcacc
     1861 aggcgaggca atcgtcgact tccttggtct gctcggccac atggatatgg accggggcca
     1921 ccgtgtcgat ccctgccagg acctcgttca ggagttcggc cgtgacagcc cgcagcgagt
     1981 gcggcgagag gccgaggcgg cgcagcggat ggccggtgac ggcgccacgc agggtttcct
     2041 ggatcttcag gtagcgattg gcgtcattga cgaagcggcg ctgcccggcg atcgcaggct
     2101 gcccgccgaa gccgccgtga ttgtagagtg tcggcagaag ggtgatggcg atccccgccg
     2161 tctctgccgc ggccaggcag cggagcgaga gttcggccgg attggcatag ggcaccccgt
     2221 ccggctgatg atgcaggtac tggaactcgc ccaccaccga gaatccggac ttcagcatct
     2281 cgacatagag ctgggcggca acggcttcga ggtctgccgg acccagcttg agggcgaagc
     2341 cgtacatgac ttcccgccag gtccagaaac tgtcagcccc cggaccggcc ttctcggcca
     2401 ggcccagcat gaagcgctga tgggcgtggg aatgaacatt ggggaccgcg ggaactgcaa
     2461 tgccgctgac ttgctgcccc tccttgccgc cgggcgtcac ggcggcgatc atgcccccgg
     2521 catcgacggc aacctcgaca tcctcctgcc agccattggc ggttagcgcg gagggaaacc
     2581 ggaacttggc gcttttcatg ggggcgatca tactgtatag acaaccttat acaagcggat
     2641 ttttgcatgc tcctcaccaa tctccaggcg gcgaccatga ctggcggcta cgggatcgtc
     2701 gccaaggcgg cggtgctgat cgagaacggc cgtatcgcat gggtcggccc ggcggcagag
     2761 gcgccttcgg gatccgccat cgactgcggc ggccgcctgc tgacgcccgg cctggtcgac
     2821 tgccacaccc atcttgtcta tggcggcaac cgcgccaatg aattcgagct ccggctgacc
     2881 ggcgtgccct atgccgagat cgccaaggcg gggggcggca tagcagccac tgtccgcgcc
     2941 acccgcaccg aatccgaggc gccgttgctg gcgtcagcat taaagcgact cgatactctg
     3001 cttgcggaag gcattacaac aatcgaggta aaatcgggct acggtcttga tatcgacacc
     3061 gaactgaaaa tgctgcgggt ggcgcggcaa cttggccagg accggccggt cgatgtccgc
     3121 acgaccttcc tcggcgccca caccttcccg ccggactaca gagaccggcg cgccgactac
     3181 gtccgtctcg tctgcgatca agccctgcct gcggcggcca aggccggcct tgccgatgct
     3241 gtcgacgcct tctgcgaaag cctcgcctt
//
LOCUS       DBNGVR010000054         3739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3554385, whole genome shotgun sequence.
ACCESSION   DBNGVR010000054 DBNGVR010000000
VERSION     DBNGVR010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3554385"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="AC16A0_01100"
     CDS             <1..597
                     /locus_tag="AC16A0_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3103952.1"
                     /translation="GYTAMLCVMALEEQGVKPADGEVLVTGAAGGVGTTAIVILAHLG
                     YKVIASTGRVSEEPFLKSLGAAGVVDRNEFNTPVKPLAKSRWAACIDSVGSVTLANVI
                     SQMNPEGTVAACGLAQGMDLPTTVAPFILRGVKLIGVNSVSTPIPRRMEAWSRIARDL
                     DLAKLKELTTHVKLDDVPRVAADIVAGQVRGRVVVDIR"
     gene            601..1233
                     /locus_tag="AC16A0_01105"
     CDS             601..1233
                     /locus_tag="AC16A0_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3103953.1"
                     /translation="MSLTLILAGIAIGLAMSAPLGPVNIIVIRAALGRGFWPAFVFGL
                     GAVIADVLFAAVAAYGVRSVEQVIHAFAVPLTLAGGVLLVVIGVRTAQKHIALSEIGT
                     TEPQGKGAMLRSAAGTFALTAFNPGTLFGYLAIFGAMSPALKLSLSPERPLLVVLGVA
                     MGGTLWWAFISFAMDRLKARLTESTLDRISRWTGVLIAAFGFALLLDLVL"
     gene            complement(1236..1496)
                     /locus_tag="AC16A0_01110"
     CDS             complement(1236..1496)
                     /locus_tag="AC16A0_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103954.1"
                     /translation="MLKTELDSDHAMTSAEAKHIALRYLMEAWEEAVYDGVDPDCLAT
                     AAIFAALSDMIATYGEEPVAAMCERLPERIRAGEFTLTKTRQ"
     gene            complement(1630..2055)
                     /locus_tag="AC16A0_01115"
     CDS             complement(1630..2055)
                     /locus_tag="AC16A0_01115"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3103955.1"
                     /translation="MTGAQQFPKLGASACVWRDGHVLLIERARPPLGLWSLPGGHVEA
                     GETVIDAARRELMEETGITAALTALVGLYEVIRHDSSGTLTAHYAIACYTGLAGEGVA
                     VAASDARDLRWVLPGDIGRLPLAPNVKDAILRARSLLSL"
     gene            2073..2744
                     /locus_tag="AC16A0_01120"
     CDS             2073..2744
                     /locus_tag="AC16A0_01120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR3103956.1"
                     /translation="MCGLYSFRKSAEETRSLFDYLDAPDFPPRTYVAPGGPIAIVRME
                     QGQRRFALVRWGFIPSWVKELKPGKPLINARGETVAEKPSFRHAFRRRRCLIPADGFY
                     EWLGDVPGKKIPYFIHRADDGLFAFAGLWEHWMSPDGSEIETGAIVTTAPNREVAEIH
                     DRMPVVIQPQDYETWLTGEPHAAAKLIHPAPDGFFTLEPTRIERNAPPPRARVEKASP
                     QMKLL"
     gene            complement(2763..3233)
                     /locus_tag="AC16A0_01125"
     CDS             complement(2763..3233)
                     /locus_tag="AC16A0_01125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007629304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3103957.1"
                     /translation="MSADITVRRYRPPDLDAAIAIYQSAIRDVASRDYDPVQIAGWSA
                     VDRDDWEPWRLRCPTWIAEVDEVPAGFSDLEPDGHLDMMFVHGRFQGRGVATALLNEV
                     EAMARRLCLGRIFTEASITARPFFARRGFTVIAPQVLEFEGVTFKNYRMEKLLG"
     gene            complement(3230..3646)
                     /locus_tag="AC16A0_01130"
     CDS             complement(3230..3646)
                     /locus_tag="AC16A0_01130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HIR3103958.1"
                     /translation="MTAFRKIEDRDVEAVVELWSACGLTRPWNDPYKDISFAREGRES
                     TILVAERDGRIIASVMAGHDGHRGMLYYVAVDPALRREGLGKAAVRAAEGWLGQRGVW
                     KINLLVRSENDAVRGFYEALGYEVSPVFCMARKIAP"
     gene            complement(3643..3726)
                     /locus_tag="AC16A0_01135"
     CDS             complement(3643..3726)
                     /locus_tag="AC16A0_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3103959.1"
                     /translation="MELEMMRGLKRLFDPNTILNPGRVLPE"
BASE COUNT          629 a         1210 c         1241 g          659 t
ORIGIN      
        1 ggctataccg caatgctctg cgtgatggcg ctggaagagc agggcgtgaa gccggcggat
       61 ggcgaggtgc tggtgaccgg tgcggcgggc ggcgtcggca ccacggcgat cgtgatcctc
      121 gcgcatctcg gctacaaggt gatcgcctcg acgggccgcg tgtcggaaga gcccttcctc
      181 aagagcctcg gtgccgcggg cgtggtggac cgcaacgagt tcaacacgcc ggtgaagccg
      241 ctggccaagt cgcgctgggc cgcctgcatc gactcggtcg gatcggtgac gctggccaat
      301 gtgatctcgc agatgaatcc ggagggcacg gtggcggcct gcggcctggc gcagggcatg
      361 gatctgccga ccacggtggc gcccttcatc ctgcgcggag tcaagctcat cggcgtcaac
      421 tcggtgagca cgcccattcc gcgccgcatg gaggcctggt cgcgcatcgc ccgcgatctc
      481 gacctcgcca agctcaagga actgaccacg catgtgaagc tcgatgacgt tccgcgcgtg
      541 gccgccgaca tcgttgcggg acaggtgcgc ggccgggtcg tggtcgacat ccgctaggcg
      601 atgagcctca cgctgatcct ggccggcatc gccatcggcc tggcgatgtc cgcgccgctg
      661 gggccggtca acatcatcgt gatccgcgcc gcattgggcc gcggcttctg gccggctttc
      721 gtctttggtc ttggcgcggt gatcgcggat gttctcttcg cggcggtcgc ggcctatggc
      781 gtgcgctcgg tggagcaggt catccatgcc ttcgcggttc cgctcacgct ggcgggcggc
      841 gtgctgctgg tggtgatcgg cgtccgcacg gcgcagaagc acattgcgct gtcggagatc
      901 ggcacgacgg agccgcaggg caagggcgcc atgctgcgca gtgcggcagg aacattcgcg
      961 ctgacggcct tcaatcccgg cacgctcttc ggctatctgg ccatcttcgg ggcgatgagc
     1021 ccggcgctca aactgtcgct ctcgccggag cggcccttgc tggtggtgct cggcgttgcc
     1081 atgggcggca cgctgtggtg ggccttcatc agcttcgcca tggaccggct gaaggcacgg
     1141 ctgactgaaa gcacactgga ccggatcagc cgctggaccg gcgtgctgat cgccgccttc
     1201 ggcttcgcgc tgctgctgga cctcgtactg taggactact ggcgggtctt ggtgagggtg
     1261 aattcgccgg cacgaatgcg ctccggcaac ctttcgcaca tggccgccac cggctcttca
     1321 ccataggtcg cgatcatgtc ggagagggcg gcgaagatcg ctgcggtcgc caggcaatcg
     1381 ggatcgacgc cgtcgtagac agcttcctcc caagcttcca tcaggtatcg aagggcaata
     1441 tgcttcgcct cggccgacgt catggcatgg tcggaatcaa gttcggtttt caacatggcg
     1501 tccaggcttc aagctgcatc ccaaccatcg actctgcggt caacggtcat ccatacccac
     1561 attagcaatt gccgggcggg cggcgactcg atccttaaag gaaggttaac gccaaagctc
     1621 tcattttagt caaagactta ggagagatcg cgcacgcagg atggcgtcct tgacgttggg
     1681 agcaagcggc aatctgccaa tatcgcctgg caggacccag cgcaagtccc gcgcatcgct
     1741 tgccgcgact gccacgccct cgcctgccag tcccgtgtag caggcgatgg catagtgcgc
     1801 cgtcagcgtc cctgacgagt cgtggcggat gacctcatag agccccacca aggccgtcaa
     1861 tgcggcggtg atgccggttt cctccatgag ctcacggcgt gcagcgtcga taacggtctc
     1921 tcccgcctcg acatgaccgc ccggcaggct ccagagaccg agcggcggcc tcgcgcgctc
     1981 gatgagcagc acatggccgt cgcgccagac gcaggccgag gcgccgagct tggggaattg
     2041 ttgtgcgccg gtcatcggtc tagattgtct ccatgtgcgg actttatagc ttcaggaaga
     2101 gtgcggagga aacccgcagc ctgttcgact atctcgatgc gcccgacttc ccgccccgca
     2161 cctatgtcgc gccgggcgga cccatcgcca tcgtgcgcat ggagcagggg cagcgccgct
     2221 tcgccctggt gcgctggggc ttcattccat catgggtgaa ggagctgaag cccggcaagc
     2281 cgctgatcaa cgcacgtggc gagacagtgg ccgagaagcc gtccttccgc catgccttcc
     2341 ggcgccgccg ttgcctgatc ccggcagatg gcttctacga gtggctgggc gacgtgccgg
     2401 gcaagaagat cccctacttc atccatcgcg ccgacgacgg gctcttcgcc ttcgccggac
     2461 tgtgggagca ctggatgtcg cccgacggtt ccgagatcga gacgggggcg atcgtcacca
     2521 cggccccgaa ccgcgaggtg gcggaaatcc acgaccgcat gccggtggtg atccagccgc
     2581 aggactacga gacatggctg accggcgaac cgcatgcggc cgccaaactg atccacccgg
     2641 caccggatgg cttcttcacg ctggagccga cacgcatcga acgcaacgcg ccgccaccca
     2701 gagcaagagt ggagaaggct tcgccgcaga tgaagctgct gtaagcagcc agcggcggca
     2761 catcagccga gcagcttctc catgcggtag ttcttgaagg tgacgccctc gaattcgagg
     2821 acttgtggcg cgatgacggt gaagccgcgg cgcgcgaaga agggacgcgc ggtgatgctg
     2881 gcctccgtga agatccgccc gaggcagagg cggcgggcca tggcctcgac ctcgttcagc
     2941 agggccgttg caacgccgcg tccctgaaag cggccgtgca cgaacatcat gtcgagatgg
     3001 ccatccggtt cgagatccga gaatccggcg ggtacttcgt ccacctcggc aatccatgtg
     3061 gggcagcgca gccgccaggg ctcccagtcg tcgcgatcga ccgcggacca gccagcgatc
     3121 tgaacggggt cataatcccg gctggcgacg tcgcggatgg cgctctggta gatggcgatg
     3181 gctgcatcca gatcgggcgg acgatagcgc cgcacggtga tgtccgcgct catggcgcaa
     3241 ttttccgggc catgcagaag acggggctca cctcgtagcc gagcgcctcg tagaagccgc
     3301 gcacggcgtc gttctcgctg cgcaccagga ggttgatctt ccagacgccg cgctggccga
     3361 gccagccttc tgccgcgcgc accgcggcct ttcccaagcc ttcgcggcgc agggccggat
     3421 ccaccgcgac gtagtagagc atgccgcgat gcccgtcatg cccggccatg acggaggcga
     3481 tgatgcggcc gtcgcgctcg gctaccagga tggtcgactc gcggccttcc cgcgcgaagg
     3541 aaatgtcctt gtacggatcg ttccacggcc gcgtaagccc gcaggccgac catagttcga
     3601 ccacggcttc gacatccctg tcctcgatct tgcggaacgc cgtcattcgg gcagcactcg
     3661 gccgggattg aggatggtat tgggatcgaa gaggcgtttc agtccgcgca tcatctcgag
     3721 ttccaccggg ctcttgatc
//
LOCUS       DBNGVR010000055         5424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3947888, whole genome shotgun sequence.
ACCESSION   DBNGVR010000055 DBNGVR010000000
VERSION     DBNGVR010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3947888"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16A0_01140"
     CDS             complement(<1..240)
                     /locus_tag="AC16A0_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR3103960.1"
                     /translation="MVSLKGTNQEFGRPYNRRIVLEAIRLHGPVERAEIARRVGLTVQ
                     TVANITHELEAQGFIKGSRSEPRGRGSPATSLAINP"
     gene            610..1743
                     /locus_tag="AC16A0_01145"
     CDS             610..1743
                     /locus_tag="AC16A0_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3103961.1"
                     /translation="MKKWLFVVIGLALLAAAAVAVRQTGVDVVAMAGFKPSNGTITKA
                     EGAKPAGGQRNAQAGGRGPLPVEIAKATSARLSDDIGAIGTLLADETVEIAPETSGRV
                     DAVLFKDGEQVVEGQPLFRLDTDLAQADLAEAKARLSLAEANYKRSQTLRKSGNVAQS
                     VYDAAVTELEVARTAVESAQVRIDKLTILAPFSGTAGFRTISAGAYVTAGTALVQLDK
                     IDLLKVSFSVPELEQSRIRLGQIIDVTADAVPGQRFTATVSAIDPSVDVNGRALLVRA
                     DLDNQDLKLRPGLLVRVTIKGAAREAVMVPETAIVQRGDNAYVYLARDAKAEEMQVRV
                     GKRMAGNIEIIEGVAAGAEVVVAGNTRLSNGAAIEVVAPTSAE"
     gene            1748..4810
                     /locus_tag="AC16A0_01150"
     CDS             1748..4810
                     /locus_tag="AC16A0_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3103962.1"
                     /translation="MGFTELCIRRPVFATVLSLVLVLAGLMSFNRLTIREYPNIDEPQ
                     VTVVTSYTGASAEIVESQVTQVLEGSLAGIEGIDTIESTSRAESSRITIRFRNTVDIN
                     AATSDVRDRVSRVRNLLPDEISEPNISKVEADAQPIIFLVMQSTSMNALELTDYVDRF
                     VVNRFKNLDGVADVTINGERRYAMRIWIDPSRLAGYGLTVQDVENAIRNQNADIPAGR
                     IESTEREFTVLSRTALATPAEFERIVLKPAGGLQVRLGDVARVELSSADVRREGRYNG
                     KTAISIGIVKQAVANPLDVSREVRAILPRLNADLPEGVSASIGFDSTVFIDRSIENVF
                     ITILEAVALVLIIIFLFLHSFRAALIPIVTIPVSLIATFAIMYATGLSVNTLTLLAMV
                     LAIGLVVDDAIVVLENIYRHIEDGMKPFEAAITGAREIGFAVIAMTLTLAAVYAPIAF
                     TPGRTGRLFLEFAITLAGAVIVSGFVALTLTPMMCSKLLRHAEKKNLFSRVVEGVLGG
                     LERGYRSSLSAALRLRWLVLLMAVGVAGAGGYVLSQMKSELSPVEDRGTITITGNAPE
                     GASLAYTQRYAGQVEQILAGVPELSSYLVIVGAGEVTRFLTFARLKDWDEREVKQQQI
                     TQSLLPKLRKIAGVQASASNPASLGVRGFGKPLQFVIQSAASYEEINALADKLVERLR
                     DNPGLADLDTDMRLNKPEVEVAIDRDRVADLGLDISVIGRTLETLLGGRNVSTFQIGT
                     EQYDVTVALPPDERTSPETLSRIFVRSANGDMVQLSNVVKARVSVAPRDLRRFNQLRA
                     ITIEANLAPGYTMGEAIADVAAAAAEVLPEGTATDLTGQAREFRDASSNLAFVFVLAL
                     AFIYLVLAAQFESFRDPLMIMVTVPLSMTGALTALWLTGGTLNVYSQIGLVTLIGLIT
                     KHGILIVEFTNSLQGQGRERRSAIIEAAALRLRPILMTTAAMILGAVPLALATGAGAE
                     SRQQIGWVIVGGMGLGTILTLYVVPCIYAVMGYRPKSAVMPEAATATA"
     gene            complement(4879..5172)
                     /locus_tag="AC16A0_01155"
     CDS             complement(4879..5172)
                     /locus_tag="AC16A0_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103963.1"
                     /translation="MESLLPIIIQLIAGAIGGNAAGAAMKGKSLGGGGNSIAGAIGGV
                     VLGQIVERMTGGAVPADAVASSGLDIGSLITNLISSGAGGAILTAIIGMIKNR"
BASE COUNT          963 a         1708 c         1697 g         1056 t
ORIGIN      
        1 gggattgatg gcgaggctgg tcgccggcga cccccgtccg cgcggttcgc tacgcgaccc
       61 tttgatgaag ccctgcgcct ccagctcgtg ggtgatattg gcgacagtct gtaccgtaag
      121 ccccacacgg cgggcgattt cggcccgctc caccggtccg tgcagccgga tggcctcaag
      181 cacgatgcgc ctgttgtagg gacgtccgaa ttcctggttc gtacctttga gggagaccat
      241 gtctccaatc cccgccctgc caccaggaga gattaggatc gggagtttta ataatgcaaa
      301 tggtttttat aattcttcgc tggcggatga atgggcgtga gcccaagatc tgtcacgggg
      361 cgacactcgt aagggctatg cgctgtcgtg ttccatcact agacttgccg ccggacacga
      421 tgatttgaac gggctgcacg gcggcgctgc cataagtctt ttgaacggga tcagactgtt
      481 tgcgggatcc gaggtgtatc gacggaatcc gttttcctgc ccgtcctaaa tcagcgggtc
      541 aaaccgtgtc gaatgggcgg cacgcggttt ctggaagtaa ccgattgcag ggcttgaggt
      601 cttgaggcga tgaagaagtg gctgtttgtc gtgatcggac tggcgctgct ggctgcggcg
      661 gccgttgccg tgcggcaaac cggcgtcgat gtcgtcgcga tggccggctt caagccttct
      721 aatggcacca tcaccaaagc cgagggcgca aaaccggctg gcggccagcg caacgcccag
      781 gctggcggac gcgggccgct gccggtcgag atcgccaagg ccacctcggc ccggctcagc
      841 gacgacatcg gcgccatcgg cacgctgctg gctgatgaaa ccgtcgagat tgcaccggaa
      901 accagcggcc gcgttgacgc ggttctgttc aaggacggcg agcaggtcgt tgagggtcag
      961 ccgctgttcc gcctcgatac cgatctcgcc caggccgatc tggctgaggc caaggcccgc
     1021 ctgtcgctgg ccgaagcgaa ctacaaacgc tctcagaccc ttcgaaagtc aggcaatgtg
     1081 gcgcagagcg tctatgatgc ggcggtgacc gagctggaag tggcgcgcac tgcagtcgaa
     1141 tccgcccagg tgcggatcga caagctcacc atcctcgcgc ccttttccgg aacggccggc
     1201 ttccgcacga tttccgccgg agcctatgtg acggccggga ccgcgctggt gcagctcgac
     1261 aagatcgatc tgctgaaggt gagtttctcg gtgcccgagc tcgagcagtc gcgcatccgc
     1321 cttggccaga tcatcgatgt cacggctgat gccgtgcccg gccagcgctt caccgccacc
     1381 gtttcggcca tcgatccctc ggtcgatgtc aatggccgcg cgttgctggt gcgtgccgat
     1441 ctcgacaatc aggatctgaa gctccggccg ggcctgcttg ttcgcgtcac catcaagggt
     1501 gcggcgcgcg aagcggtgat ggtgccggag acggccatcg tgcagcgcgg cgacaatgcc
     1561 tatgtctatc tggcgcgcga tgccaaggca gaggagatgc aggtccgcgt cggcaagcgc
     1621 atggcaggca acatcgagat catcgagggc gtggctgccg gtgccgaagt ggtggtggcc
     1681 ggcaatacgc gcctgagcaa tggcgccgcc atcgaggtgg ttgcacccac ttcggcggaa
     1741 tagcgccatg ggcttcaccg aactctgcat ccgccgcccg gtgttcgcta cggtgctgag
     1801 cctcgtgctg gtgctcgcag gtctcatgtc cttcaaccgc ctgaccatcc gcgagtaccc
     1861 aaacatcgac gaaccgcagg tcacggtggt gaccagttat accggcgcct ctgccgagat
     1921 cgtggaatcg caggtgaccc aggttctgga gggatcgctc gcgggcatcg agggcattga
     1981 taccatcgaa tcgacgtcgc gcgccgaatc cagccgcatc acgatccgct tccgcaacac
     2041 cgtcgacatc aacgcggcaa ccagcgatgt ccgcgaccgt gtgagccgcg tccggaacct
     2101 gctgcccgac gagatcagcg aacccaacat ctcgaaggtc gaagccgacg cgcagccgat
     2161 catttttctg gtgatgcagt cgaccagcat gaatgcgctc gaactcaccg actatgtcga
     2221 ccgcttcgtt gtcaaccgct tcaagaatct cgacggcgtt gcggacgtga cgatcaatgg
     2281 cgagcgccgc tacgccatgc gcatctggat cgacccgagc cgcctggccg gttacgggct
     2341 caccgttcag gatgtcgaga acgcaatccg caaccagaat gccgacattc cggcaggccg
     2401 catcgagagt accgagcgcg agttcaccgt gctgtcacgg accgccttgg caacgccggc
     2461 cgagttcgag cgtatcgtgt tgaagcccgc cggaggcctg caggtcaggc tcggcgacgt
     2521 ggcgcgtgtc gagctgtcca gcgccgatgt gcggcgcgaa ggccgctaca acggcaagac
     2581 cgcgatctcc atcggcatcg tgaagcaggc ggtggccaat ccgctggatg tgtcgcgcga
     2641 ggtgagggcc atcctgccgc gtctcaatgc cgaccttccg gaaggcgtgt cggcctcaat
     2701 cggcttcgat tcgacagtct tcatcgatcg gtccatcgag aatgtcttca tcaccattct
     2761 tgaggccgtc gcactggtgc tcatcatcat cttcctcttc ctgcattcct tccgcgcggc
     2821 cctcattccg atcgtcacca ttccggtctc gctgattgcg acctttgcca tcatgtatgc
     2881 cacgggcctc tcggtgaaca ccctgacgct gctggccatg gtgctggcca tcggcctcgt
     2941 ggtggatgat gccatcgtgg tgcttgagaa catctatcgc cacatcgagg acggcatgaa
     3001 gcccttcgaa gcggcgatca cgggcgcacg cgagatcggc ttcgcagtga tcgccatgac
     3061 gttgacgctt gccgccgtct atgcgcccat cgccttcact cccgggcgaa ccggcagact
     3121 gttcctggag ttcgccatca cgctggccgg cgcggtgatc gtttcgggct tcgtggcgct
     3181 gacgctgacc cccatgatgt gctccaagct cttgcgccac gcggagaaga aaaacctgtt
     3241 cagccgggtt gtcgaagggg ttctgggcgg tctggaacgt ggctaccggt ccagcctttc
     3301 cgcggccctg cgcctgcgct ggctggtgct tctcatggcg gtcggggtgg ccggtgcggg
     3361 cggctatgtc ctctcccaga tgaagtcgga actgtctccc gttgaagacc gtggcaccat
     3421 cacgatcacc ggaaatgcgc cggagggggc cagcctcgcc tacacccagc gctatgccgg
     3481 acaggtcgag cagattctgg ccggggtgcc cgaactcagt tcctacctgg tgatcgtcgg
     3541 ggcaggcgag gtcacgcgct tcctgacctt cgcgcgtctc aaggactggg acgaacgtga
     3601 ggtcaagcaa cagcagatca cgcagtcgct cttgccaaaa ctgcgcaaga ttgcgggtgt
     3661 tcaggcgtcg gcctcgaacc ccgcatcgct gggcgtccgc ggcttcggca agccgctgca
     3721 gttcgtcatc cagtctgccg cttcttatga agagatcaac gcgcttgcgg acaagctggt
     3781 cgagcggctg agggacaatc caggcttggc cgatctcgat accgacatgc gtctcaacaa
     3841 gccggaggtc gaagtagcca tcgatcgcga ccgcgtggcg gatctcggtc tcgacatctc
     3901 ggtgatcggg cgcaccctcg aaaccctgct tggcggccgc aatgtctcga ccttccagat
     3961 tggtacggaa cagtatgacg tcacggtggc gctgccgccg gacgagcgga catcgccgga
     4021 aaccctctcg cgcatcttcg tgcgcagcgc caatggcgac atggtgcagc tgtcgaacgt
     4081 ggtcaaggcc cgggtctcgg tggcgccgcg cgatctccgc cggttcaacc agctccgcgc
     4141 catcacgatc gaggccaatc tggcgccggg atacacaatg ggcgaagcca ttgctgatgt
     4201 cgccgcggca gcagccgagg tgctgcccga gggaaccgcg acggacctca ccggccaggc
     4261 ccgcgagttc cgcgacgcca gttccaatct ggctttcgtc ttcgtgctgg cgcttgcctt
     4321 catctacctc gtgcttgccg cgcagttcga aagttttcgg gatccgctga tgatcatggt
     4381 gaccgtgccc ttgtcgatga ccggcgcgct caccgcgctg tggctgaccg gcggcacgct
     4441 gaacgtttat tcgcagatcg gcctggtgac gctcatcggg ttgatcacca agcacggcat
     4501 tctcatcgtg gagttcacca acagcctgca gggccagggc cgcgagcggc ggtcggccat
     4561 catcgaggcg gcggcgctgc gcctgcggcc gatcctgatg acgacagctg ccatgatcct
     4621 tggcgctgtt ccgctggcct tggcgacagg tgcgggtgcc gaaagccggc agcagatcgg
     4681 ctgggtcatc gtcggtggca tgggactcgg cacgatcctg acgctctacg tggtcccctg
     4741 catctacgcc gtcatggggt acaggccaaa atccgcggtc atgcccgagg ccgcaacggc
     4801 tacggcctga caccggaaac aaaaaggccg cggccgtcat cgccggccgc ggcctctaac
     4861 gtcgatcccc gacggggatc agcggttctt gatcatgccg atgatggcgg tcaggatggc
     4921 gccaccggcg ccgctcgaga tcaggttggt gatcaggctg ccgatatcaa gtccggaaga
     4981 cgcaacggca tcggccggta ctgcgccgcc ggtcatgcgc tcgacgatct ggccaagcac
     5041 cacgccgccg atggcgccag caatcgagtt gccgccgccg ccgaggcttt tgcccttcat
     5101 ggccgcgccg gccgcattgc cgccgatcgc gcccgcgatc agctgaatga tgataggaag
     5161 taagctttcc atttagatct ccagttccac ccgaccccaa ttgtgattcc ccatggcggg
     5221 ttggtgaact agatcagagg gtgctatcta tggcaagctt caactaagca agtgcaactg
     5281 aaccttcttg caactaatcg gaactccttg ttctggttca cgatcccggt ctaggcttag
     5341 gccctttcct tgtggagcac tttggacgat cattgcactg aaaggtgctt cgcatggcgc
     5401 aattgagtat tcgaatcgat tttg
//
LOCUS       DBNGVR010000056         4604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1590406, whole genome shotgun sequence.
ACCESSION   DBNGVR010000056 DBNGVR010000000
VERSION     DBNGVR010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1590406"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /gene="pyrD"
                     /locus_tag="AC16A0_01160"
     CDS             <1..752
                     /gene="pyrD"
                     /locus_tag="AC16A0_01160"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HIR3103964.1"
                     /translation="NRMGFNNEGHAAALRRLEARSPHGIVGVNIGANKDATDRIADYV
                     AGIATFLHLASYFTVNISSPNTPGLRALQSRAELAALLSRLNDARSKQAARPPMLLKI
                     APDLRADELEDIAAACAGGAVDGIIVSNTTLRRDGLKSAARVEPGGLSGAPLFTLSTQ
                     VLAKVYRLTGGNIPLVGAGGIRDAETALQKIRAGASLLQIYSALVYAGPGLISSILDG
                     LRRHTALNGKTLTDLRGADAAFFAHQGLSGT"
     gene            complement(728..1594)
                     /locus_tag="AC16A0_01165"
     CDS             complement(728..1594)
                     /locus_tag="AC16A0_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3103965.1"
                     /translation="MTLRYARVESRQLSFLAMVTALLLTLALPARAEPYTLPQFRTVD
                     PAAAAAAQELSARSVRLLADADFPPFSFAAQGGGPSGLGVELALAACVEARLSCEVVL
                     LPYGELLAALNRGDGDAVISGLRLDETSLQDALMTRPWFRTMGRFAVLRDSPVKSSDP
                     KDLAGLRIGVIRDTAHARWLETYYGGSILVPFDDAASAQLALRIGNVQAVFGDSLQMI
                     YGLTSAAAQNCCKLLGGAYSDFDFFSRNIAFLVRAGQADLRDALDYGLDAAQTKGLTE
                     KLFNAYVPLNPW"
     gene            1734..3401
                     /locus_tag="AC16A0_01170"
     CDS             1734..3401
                     /locus_tag="AC16A0_01170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3103966.1"
                     /translation="MSTLADALRRLPEDELVIALRSKLVPIVTVAGLRLFAACGASAF
                     RMAEENGLKVVAEAAAADFLAAVRLSLGRRLADDAANGLARCRPEFSASQRLTRGQMA
                     LSIVSGALLCLGTALLPLAVTWFAASLSAALFFLSVIAVRMLCLLPPPQRHGRLTHVA
                     PADSALPIYTLLVPLHMETSVLGQLLEALLALDYPCDRLDIKLIIEESDIAMQRALAG
                     TGLPEHFDVIVVPCGLPQTKPRALNYALCFARGSLLTIYDAEDIPDPRQLRRAAAAFA
                     VLPQDTACLQAELVFDNANENWLTRQFTIEYAMLFGMILPSLAAHRLPLPLGGTSNHF
                     RIDALRRAGAWDAYNVTEDADLGIRLARLGFDTDTITSCTYEEANVSLRNWMRQRTRW
                     MKGFLATWLVHMREPLAALQELGPSGFWSMQALTIGVFASALVHPPCLIASILLVTWG
                     HGQPVPSTAADNLIAGLGLLVFVLGYAVSMIAAWRALRRYRIFGWWRDLLAMPAYWLL
                     MSVAAWLALWQFIVAPFHWNKTVHGLSRKRGSAGLSALAKDGSRVAR"
     gene            3735..3992
                     /locus_tag="AC16A0_01175"
     CDS             3735..3992
                     /locus_tag="AC16A0_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103967.1"
                     /translation="MTKRQIAKLKKLVSMAQDLLESAEANPVGPAASARRGSRPIRHR
                     RTAAEAAKMRSDILAKRAKGVSALSLAKKYNVSTAYIYMIK"
     gene            complement(4220..>4604)
                     /locus_tag="AC16A0_01180"
     CDS             complement(4220..>4604)
                     /locus_tag="AC16A0_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103968.1"
                     /translation="PELPESLLAPDDPPEPLPLFPPLLPLLPPPPPLLPPLLLPLLPP
                     PPVPELPPPDACAWLMFMTAGATYTAVASSPSFLMISRRSMRRPPILFSLKNQDCVIL
                     PDRNLTRTGKSSSWQIYRIFSLMNR"
BASE COUNT          822 a         1435 c         1445 g          902 t
ORIGIN      
        1 tcaatcgcat gggcttcaac aacgagggcc atgctgcggc cctgcggcgc ctcgaagccc
       61 gttcgccgca cggcatcgtg ggcgtcaata tcggagccaa caaggatgcc accgaccgca
      121 tcgccgacta tgtggcgggg atcgccacct tcttgcatct cgcctcctat ttcaccgtga
      181 acatctcgtc gcccaacacg ccgggcctgc gggcgctgca atcgcgggcc gagctggcag
      241 ccctgctgtc gcgactcaat gacgcccgaa gcaagcaggc ggcgcggccg ccgatgctgc
      301 tcaagatcgc gccggacctg cgtgcggacg agctggaaga catcgccgcg gcctgcgcgg
      361 gcggggcggt ggacggcatc atcgtctcca acaccacctt gcgccgtgac ggcttgaagt
      421 ccgccgcgcg ggtggaaccg ggcgggcttt ccggcgcacc cctgttcacg ctgtccaccc
      481 aggtgctggc caaggtctat cgcctcaccg ggggcaacat tcctctggtt ggcgctggcg
      541 gcatccgcga tgccgaaacg gcgctgcaaa agatcagggc cggcgcaagc ctgctgcaga
      601 tttactcggc gctggtctat gccgggccgg gattgatcag ttcaattctc gacggcttgc
      661 gccgccacac tgcactgaac ggcaagaccc tgacagacct gcgcggggcc gatgccgcgt
      721 tcttcgctca ccagggattg agcggcacat aggcattgaa cagcttttcc gtcaggccct
      781 tggtctgggc tgcgtcgagt ccgtagtcga gagcgtcgcg caggtcggcc tgtccggcac
      841 gcaccaggaa ggcgatgttg cggctgaaga agtcgaaatc cgaataggcc ccgcccagca
      901 gcttgcagca gttctgcgcc gccgcgctgg tcagcccgta gatcatctgc aggctgtcgc
      961 caaacaccgc ctgcacattg ccgatgcgca gcgccagctg ggccgaggcc gcatcgtcga
     1021 agggcaccag gatcgagccg ccataatagg tttcgagcca gcgggcatgc gccgtgtcgc
     1081 ggatcacgcc gatgcgcagg ccggcgaggt ccttggggtc ggacgacttg accgggctgt
     1141 cgcgcagcac tgcaaaccgt cccatggtcc ggaaccaggg cctggtcatc agggcgtcct
     1201 gcaggctggt ctcgtccagc ctgagacccg aaatgaccgc atcgccgtcg ccgcggttca
     1261 gcgcggcgag caattcgcca tagggcagga ggacaacctc gcaggagagc cgtgcttcaa
     1321 cgcaggccgc cagcgccagc tccaccccaa gtccggacgg tccaccgccc tgggccgcaa
     1381 aactgaaggg cgggaaatcg gcatcggcca gcagcctgac gctgcgggcc gagagttcct
     1441 gcgcggctgc ggcggccgcg ggatccacgg tgcggaactg gggcagcgtg tagggctcgg
     1501 ccctggccgg cagcgccagg gtcagcagca gggcagtcac catcgccaga aagctcaatt
     1561 ggcgcgattc aactcttgcg taacgtaagg tcatttatac aatacagagt tgcaacatgg
     1621 tgggctcagg cttgtcatat ccggcagacg atggggagcg aactcccttt agtagagcgt
     1681 cgattggcag aaccatagac cggcgcaatc ggcaagtctt gccacagctt gatgtgtcca
     1741 cacttgctga cgctctacgc cgcctgccgg aagacgagct ggtgatcgcg ctgcggagca
     1801 aactggttcc catcgtcacg gtcgccggac tgcggctctt cgcggcctgc ggtgcttcgg
     1861 ccttccgcat ggctgaagaa aatggcctta aggtggtggc tgaggccgcg gcggcggact
     1921 ttcttgccgc cgtgcggttg tcgctgggcc gcaggctggc tgatgatgcg gcgaatgggc
     1981 tcgcccgttg ccggccggaa ttctcggcca gccaaaggct gacccgcggg cagatggcgc
     2041 tgtcgatcgt gagcggagct ctgctctgcc ttggtaccgc gttgcttccg cttgccgtga
     2101 cctggtttgc ggcaagcctc tcggcggcgc tgttctttct ttcggtcatc gcggttcgca
     2161 tgctctgcct tctgccgccg ccgcagcggc acgggcggct gacgcatgtc gctcccgccg
     2221 acagcgcgct tcccatctac accctgctgg ttccgcttca catggaaacc tccgtgctcg
     2281 gacagttgct ggaggcgctg ctggccctcg actatccctg cgatcgcctc gacatcaagc
     2341 tgatcatcga ggaaagcgac atcgcgatgc agcgtgcgct cgccggcacc gggctgccgg
     2401 agcatttcga cgtgatcgtc gttccttgcg gcttgccgca gaccaagccg cgcgcgctca
     2461 actacgccct gtgcttcgcg cgcggcagcc tgctcacgat ctacgatgcc gaggacatac
     2521 ccgatcctcg ccagctgcgc cgtgccgcag cggcctttgc tgtcctgccc caggatacgg
     2581 catgcctgca ggccgaactg gtcttcgaca atgcgaacga gaattggctg acccgtcagt
     2641 tcaccatcga atatgcgatg ctcttcggca tgatcttgcc gtcccttgcc gcccatcgcc
     2701 tgccgctgcc ccttgggggg acctcgaacc acttccgcat cgatgccctg cgccgggccg
     2761 gtgcctggga cgcctacaac gtgacggagg atgccgatct tggaatccgc ctcgcgcgac
     2821 tcggcttcga cacggacacg atcacgtcct gcacatacga ggaggccaat gtgagtctgc
     2881 gcaactggat gcgccagcgc acacgctgga tgaagggctt ccttgcgacc tggctggtcc
     2941 acatgcgcga acctctcgcc gcgctgcagg aacttgggcc atcgggattc tggtcgatgc
     3001 aggcccttac cattggtgtc tttgcatccg ctttggtcca cccgccatgc ctgatcgcat
     3061 cgatcctgct ggtgacgtgg ggccatggcc agccagtacc atcgacggcc gcggacaatc
     3121 tgatcgccgg tctcggcctg cttgtcttcg tgctgggtta tgccgtgtcg atgattgccg
     3181 cttggcgtgc cttgcgtcgc taccgcatct tcggatggtg gcgcgacctt ttggcgatgc
     3241 cggcttactg gctgctgatg tcggtcgccg cctggcttgc gctctggcaa ttcatcgtcg
     3301 cgccctttca ttggaacaag acggtgcatg gactttcgcg aaagcggggc agtgccggac
     3361 tttccgctct cgcgaaggac ggcagccgtg ttgcgcgcta gggtggaagt tggtttggac
     3421 ttcgtattgc gcctgaatgt tactaaaaca cataggaagc aactgctggt gttgtccacc
     3481 aattacgcaa cgtgaaatct attcgacttg cttgcacact acgacttgaa caatcttcat
     3541 gcagcttgat cagtgttcgc tcaatcatgc gctgacggtc tagcaaacga tttgccttcg
     3601 acttgccatg cgcagcttga cacaattttg cccatacgtt gacttagcat gcaagggttg
     3661 catcaaaaga agttgcgaag catggacgca ctgcttagga agatggtgct ttaaacaaac
     3721 ttatggatgg aactgtgacc aaacggcaga ttgctaagtt gaagaagttg gtttcgatgg
     3781 ctcaggatct tcttgaatcc gcagaagcca acccggttgg ccccgctgca agcgcacggc
     3841 gcggcagccg cccgatccgc catcgcagga ccgcggcgga ggcggccaag atgaggagcg
     3901 atattcttgc caagcgcgcc aagggcgtat cagcgctctc gctggccaag aagtacaatg
     3961 tgagtacggc ctatatctac atgatcaagt aagtgcggtg gtcgcggctt tgatgcaatg
     4021 gcgttgccgc aagctgaaaa cgatttctag gtcacaatct gcataagccc aggttcaggt
     4081 tccggcgcgg acctggcctg cgccttcgct tgcgacgcta tccggaacct gtcgcttgaa
     4141 cgcgccggtt gccacttggg cgaacggtca agaggtcgtt gtgcagacct tcgattttcg
     4201 acagatcttc cggcagtcgt taacggttca ttaggctgaa gatccggtag atctgccagg
     4261 aactcgattt cccggtcctt gtcaggtttc tgtcaggtag aatcacgcaa tcttggttct
     4321 tgaggctaaa caagattgga ggtctcctca tggatcggcg tgaaatcatc aagaagctcg
     4381 gactgcttgc gacggccgta tacgtcgccc ccgcggtcat gaacatcagc caggcgcatg
     4441 cgtctggcgg cggcaactcc ggcaccggag gtggcggcag cagcggcagc agcagcggtg
     4501 gcaacagcgg cggcggaggt ggcagcagcg gcagcagcgg cggaaacagc ggcagcggct
     4561 cgggcggatc gtccggcgcg agcagcgatt cgggcagttc aggt
//
LOCUS       DBNGVR010000057         2173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1984168, whole genome shotgun sequence.
ACCESSION   DBNGVR010000057 DBNGVR010000000
VERSION     DBNGVR010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1984168"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="AC16A0_01185"
     CDS             <1..1086
                     /locus_tag="AC16A0_01185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3103969.1"
                     /translation="VMCEQLAEIGFACTAAENGETGREILNRSRFDLAIIDITMPKLD
                     GFGLLRHMRQHPRTVDLPAIVCTSHNDRASIERAYTLGASSFVTKPINWPQFSHHVQF
                     VLRNGETEKALRQAQTEALAASRMKNGLFQVLSHELKTPLTALIGLTEVLAEQLQGRV
                     TADERESLAHVVEGAQRLNAIVSDILLLSKALGNPNQQKLADIPITELLDDGLVGQKA
                     LARERQVSLLLRPMEDNFAVTCDVHLIRQALSRLINNAIKFSPIGGSVEVWAHRRADG
                     SILISVKDNGPGLTQAKLQDCLKPFMQEDMTYARPVDGLGLGLPIAKAICEAHGGELL
                     VQTAPGQGLVAAICLPPRLSSVLYNAA"
     gene            1192..>2173
                     /locus_tag="AC16A0_01190"
     CDS             1192..>2173
                     /locus_tag="AC16A0_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3103970.1"
                     /translation="MRDPRYDILFEPVRIGPVTAKNRFYQVPHCNGGGYRDQSAAAEM
                     RRVKSEGGWGVIFTEQVEIHHTSEITPFIELRIWDEDDMPGLAKMADAIHSNGALAGI
                     ELAYSGANGPNLYTREVPLAPSHLPILTFTSDPVQARAMDKQDIRNLRHWHKLAYRRA
                     RRAGYDILCLYGAHGFGVIQHFLSPRTNQRTDEYGGSLANRARLLKELISDARDEVGD
                     TCAIAVRLSLDELAGEQGFSNAQLRDMVAMHADLPDIWDFAHGTWEACSGTSRFKPEG
                     AQQDLIRGLKQLTPRPVVGVGRFTSADMMVRQVKQGLMDFIGAARPSIADP"
BASE COUNT          393 a          749 c          661 g          370 t
ORIGIN      
        1 gtcatgtgcg agcagcttgc cgaaatcgga ttcgcctgta ccgccgcaga gaatggcgaa
       61 accggccgcg agatcctcaa ccggtcgcgc ttcgatctcg ccatcatcga cattaccatg
      121 ccgaagctcg atggcttcgg cttgctccgc cacatgcggc agcatccgcg cacggtcgac
      181 cttccggcca tcgtctgcac ctcgcacaat gaccgcgcct cgatcgagcg cgcctacacg
      241 ctgggggcat cctccttcgt caccaagccg atcaactggc cgcaattctc ccatcacgtg
      301 cagttcgtgc tgcgcaatgg cgagaccgag aaggcgctcc gccaggcgca gaccgaggcg
      361 ctggccgcca gccgcatgaa gaacggcctg ttccaggttc tcagccatga actcaagaca
      421 ccgctcaccg ccctcatcgg cctgaccgag gtcctggccg aacagctgca ggggcgggtc
      481 acggccgacg agcgcgaaag ccttgcgcat gtggtggaag gcgcccagcg cctgaacgcc
      541 atcgtcagcg atatcctgct gctgtccaag gccctgggca atcccaatca gcagaagctg
      601 gccgacatcc ccatcaccga actcctcgac gacggcctgg tcgggcagaa ggccttggcg
      661 cgcgaacgcc aggtcagcct gctgctccgc ccgatggaag acaatttcgc cgtgacctgc
      721 gacgtccatc tgatccgcca ggccctgtcg cggctgatca acaatgccat caagttctcg
      781 cccatcggcg gcagcgtgga agtctgggcc catcgccggg ccgacggctc catcctcatt
      841 tcggtgaagg acaatggtcc cggcctcacc caggccaagc tgcaggattg cctcaagccc
      901 ttcatgcagg aagacatgac ctatgcgcgc ccggtggatg ggctgggcct cggactgccc
      961 atcgccaagg ccatctgcga ggcccatggc ggtgagctgc tggtacagac ggcgcccggc
     1021 cagggactgg tcgccgccat ctgcctgccg ccgcgcctgt cgtcggtcct ttacaacgcc
     1081 gcgtgatccc ggcctgatgc cctgacgccg cgatggcttg acgccttcgc gcggcgctgc
     1141 gaacctgtcc ccggcaaggg gagatttccg acatcatgat ggcgtcactc gatgcgtgat
     1201 ccgcgctatg acattctctt cgagccagtc aggatcgggc cggtcaccgc gaagaaccgc
     1261 ttctatcagg tgccacactg caatggcggc ggctatcgcg atcagtcggc tgccgccgag
     1321 atgcgccggg tgaagtcgga aggcggctgg ggtgtcatct tcaccgagca ggtcgagatc
     1381 catcacacgt ccgagatcac gcccttcatc gaattgcgca tctgggacga ggacgacatg
     1441 cccggcctcg cgaagatggc cgatgccatt cattccaatg gtgcgctggc cggaatcgag
     1501 ctggcctata gcggcgccaa cgggcccaat ctctacacgc gcgaagtgcc gctggcgccc
     1561 tcgcacctgc ctatcctcac cttcaccagc gatccggtcc aggcccgcgc catggacaag
     1621 caagacattc gcaacctgcg tcactggcac aagctggcct accgccgcgc caggcgggcg
     1681 ggctacgata tcctctgcct ttacggcgcg catggcttcg gcgtgatcca gcatttcctc
     1741 tcgccgcgca ccaatcagcg gacggacgaa tatggcggct cgctcgcgaa ccgcgcgcgc
     1801 ctgctgaagg agctgatctc ggacgcgcgc gacgaggtgg gcgacacctg cgccatcgcg
     1861 gtcaggctgt cgctcgacga actggcgggc gagcagggct tctccaacgc gcagcttcgc
     1921 gacatggttg ccatgcatgc agacctgccc gacatctggg acttcgccca tggcacatgg
     1981 gaagcctgtt ccggcacctc gcgcttcaag ccggaggggg cgcagcaaga cctgatccgt
     2041 ggattgaagc agctgacgcc gaggcccgtc gtcggtgtcg ggcgcttcac gagcgccgac
     2101 atgatggtgc ggcaggtcaa gcaaggcctg atggatttca tcggcgcggc aagaccttcc
     2161 atcgctgatc cgt
//
LOCUS       DBNGVR010000058         3900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2377348, whole genome shotgun sequence.
ACCESSION   DBNGVR010000058 DBNGVR010000000
VERSION     DBNGVR010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2377348"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="AC16A0_01195"
     CDS             complement(<1..1273)
                     /locus_tag="AC16A0_01195"
                     /inference="COORDINATES: protein motif:HMM:NF033792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HIR3103971.1"
                     /translation="MLFDTIPEALKTATDEELGVLRLRTLVRLRWLAIIGQTTAVLGV
                     HFYLGYPLPLGLCLAAIALSAWLNIFLAIRWRKSVRLQPSHAGFLLAYDICQLAVLLY
                     LTGGLQNPFSFLFLVPVTISATSLSLNWTLWLGATAFCCASVLAFEHLPLPWKPGEEL
                     VLPSIYIAGMWAAIVCGVVFAAIYARRIAEEARQMQAALTATELVLAREQRLSALDGL
                     AAAAAHELGTPLATIALVAKELKRELPAEGPHAEDLDLLIGQAARCREILARLANHDA
                     EADEIYQRTKLLVMIEDLIEPLRGSEVEIAVTSSANDAGKAKSEEPIFRRNPAIAYGL
                     GNLLENAVDFAGNKVEVDARWTPSSVSITVRDDGPGFRQDVFDRLGDPFVTTRPGYGE
                     ESGGKGQHDGMGLGFFIAKTLLERSGAAVSLA"
     gene            1348..1941
                     /locus_tag="AC16A0_01200"
     CDS             1348..1941
                     /locus_tag="AC16A0_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3103972.1"
                     /translation="MPTRTRLIFAAAILLLAAVIGGVALTQRGTGSGSGEALVGGPFR
                     LTNHLGQTVTEADFRGKYMLVFFGFTFCPDICPTELQVMTHALETMGASGARITPVFV
                     TIDPERDTPDVMKAYVENFGPNLVGLTGTPEEIAAVAKAYRVYYKKSGDSADYLMDHS
                     SIIYLMDPDGRFVKHFTYTTDAGALAEGLTQAMANTP"
     gene            2021..3250
                     /locus_tag="AC16A0_01205"
     CDS             2021..3250
                     /locus_tag="AC16A0_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HIR3103973.1"
                     /translation="MLYYLYEMNHAALAPWRAAADAGLSFWKSPSNPLASTQMGRSMA
                     ASLEMFERGTRRYGKPAFEITDTILGDDLVAVEEVDALQKDFCRVINFRKQWTTAPAR
                     PEHKLLIVAPMSGHYATLLRGTVEAMLPHYDVYITDWIDARTVPLSRGSFDLDDYIDY
                     VTEILHTLGPGTSVMAVCQPSVPVLAAVSLMNAAKDPCAPRSMILMGGPIDTRRNPTA
                     VNKLAEKRGIDWFRQNVVMKVPPPHAGMMRDVYPGFLQLGGFMTMNLDRHIDAHRELF
                     WHLVEGDGDSAEKHREFYDEYMSVMDLTADFYLQTVEKVFINHDLPRGTFTHRNQKID
                     PGKITQTALMTIEGERDDISGVGQTEAAQDLCSALAPEKKLHHLQEGVGHYGVFNGSR
                     FRREIVPRIVEFVNANA"
     gene            3250..>3900
                     /locus_tag="AC16A0_01210"
     CDS             3250..>3900
                     /locus_tag="AC16A0_01210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3103974.1"
                     /translation="MAGLFARFRKTPAPRPLEPGMMTIGLDQVPVQFRRHAQARRLVL
                     RLSPDGSTVVVTVPPRVSRAEAMSFAEKSRDWVEKRLKAHGGGIALAPGSRIPLRGVL
                     HEIRHVPARRGTVAVDATEALILVPGELPHLPRRLVDWMKTAARADLQLASRKYAALM
                     DVAFKRITIRDQRSRWGSCSAGGELSYSWRLILTPPHVLDYVAAHEVAHLKHMNHGP"
BASE COUNT          722 a         1288 c         1228 g          662 t
ORIGIN      
        1 ttgccagcga cacggcagcg cccgagcgct caagcagcgt cttggcgatg aagaagccaa
       61 gccccatgcc gtcatgctgg ccctttccgc cgctctcctc gccatagccg ggccgcgtgg
      121 tcacgaaggg atcgcccagc cggtcgaaga cgtcctgcct gaatccggga ccatcgtcgc
      181 ggaccgtgat ggagaccgac gacggcgtcc atcttgcatc gacctccacc ttgtttccgg
      241 cgaagtcgac ggcattctcc agcagattgc cgagcccgta ggcaatggcc gggttgcgcc
      301 ggaagatcgg ctcctccgac ttggctttcc cggcatcgtt ggccgaactc gtgactgcga
      361 tctcgacttc ggaaccgcgc agcggctcga tcagatcctc gatcatcacc agcagcttgg
      421 tgcgctggta gatctcgtcg gcctcggcat cgtgattggc aaggcgggca agtatctcgc
      481 ggcagcgcgc ggcctggccg atcagcaggt cgagatcctc cgcatgcgga ccttcggcgg
      541 gaagttcgcg cttcagttcc ttcgccacca gcgcaatggt ggccagtggc gttcccagtt
      601 catgcgcggc cgcggcagcc aggccgtcga gcgccgacag gcgctgttcg cgggccagca
      661 caagctcggt ggccgtcagt gcagcctgca tctggcgcgc ctcctcggca atgcgccgcg
      721 cataaatggc cgcgaaaacc acgccgcaga caatggccgc ccacatgccg gcaatataga
      781 tgctgggcag caccagctcc tcgcccggct tccacggcag cggcaggtgc tcgaaggcaa
      841 gcacgctggc gcagcagaag gcggtggcgc ccagccacag ggtccagttc agcgagaggg
      901 acgtggccga aatggtaacg ggcaccagga acaggaagga aaagggattc tgcagcccgc
      961 ccgtcaggta gagcagcacg gccagctggc agatgtcata ggcgagcaga aacccggcat
     1021 gtgacggttg caggcgtacg ctcttgcgcc agcggatggc gagaaagatg ttgagccagg
     1081 cactgagcgc gatggccgcg agacaaaggc ccagcggcag cggatagccg agatagaaat
     1141 gcacgccgag cacggccgtc gtctggccga tgatggcaag ccagcgcagc cgcaccaggg
     1201 tgcgcagccg cagcacgccc agttcttcgt cggtggccgt cttcagggcc tcgggaatgg
     1261 tgtcgaagag catctcatgc gccagggtcg gacagttgta ccttcgccag aataggacta
     1321 tcccgggcca atcggaagcc aatgaccatg ccgacccgga ccagactgat cttcgccgcc
     1381 gcgatcctgc tgcttgccgc agtcatcggc ggcgtggccc tgacgcagcg cgggacgggc
     1441 agcggcagcg gcgaggcgct ggtgggtggc cccttccggc tgacaaatca tctgggacag
     1501 accgtcaccg aggccgattt ccgcggcaag tacatgctgg tcttcttcgg tttcaccttc
     1561 tgccccgaca tctgcccgac cgaactgcag gtcatgaccc atgcgctgga gacaatgggc
     1621 gcatctggcg cgcgcatcac gccggtcttc gtcaccatcg atcccgagcg cgatacgccg
     1681 gacgtgatga aggcctatgt cgagaacttc ggtccgaacc tcgtgggcct caccggaacg
     1741 ccggaggaga tcgccgccgt ggccaaggcc tatcgcgtct attacaagaa atcaggcgat
     1801 tccgccgact atctcatgga ccattccagc atcatctatc tgatggaccc ggacgggcgc
     1861 ttcgtgaagc acttcaccta caccaccgac gccggggccc ttgcggaagg attgacccag
     1921 gcgatggcga atacccccta aagacctaga aatgccaggt tgccgcccat ttacagagtt
     1981 gatacccttt ggggcggaag atcggggctg agggacataa atgctttatt atctctatga
     2041 gatgaatcat gccgcgctgg cgccctggcg cgcggcagcg gatgcaggct tgtccttctg
     2101 gaagagtccg tccaatccgc tggcctcgac ccagatgggc cgctccatgg cggcctcgct
     2161 tgagatgttc gagcgcggca cgcggcgcta cggcaagccg gccttcgaga tcaccgatac
     2221 catcctcggt gacgatctgg ttgcggtcga ggaggtcgac gccctgcaga aggacttctg
     2281 ccgcgtgatc aacttccgga agcagtggac gacagccccg gccaggcccg agcacaagct
     2341 gctgatcgtg gcccccatgt ccggccacta tgccacgctg ctgcgcggca cggtggaagc
     2401 catgctgccg cattacgacg tctatatcac cgactggatc gatgcccgga ccgtgccgct
     2461 gagccgcggc agcttcgatc tcgatgacta catcgactat gtgaccgaga ttctccacac
     2521 gctggggccc ggcaccagcg tgatggccgt ctgccagcct tcggttcccg tgctcgctgc
     2581 cgtgtcgctc atgaatgccg cgaaggaccc ctgcgcgccg cgcagcatga tcctgatggg
     2641 cggcccgatc gatacgcggc gcaatcccac cgccgtcaac aagctggcgg aaaagcgcgg
     2701 catcgactgg ttccgccaga atgtcgtgat gaaggtcccg ccgccgcatg ccggcatgat
     2761 gcgcgacgtc tatcccggct tcctccagct cggcggcttc atgaccatga acctcgaccg
     2821 ccacatcgac gcgcatcgcg agctgttctg gcatctggtc gagggcgacg gggattcggc
     2881 cgagaagcac cgcgagttct acgacgagta catgtcggtg atggatctca ccgccgactt
     2941 ctatctgcag accgtcgaga aggtcttcat caaccacgat ctgccgcgcg gaaccttcac
     3001 tcatcgcaat cagaagatcg atcccggcaa gatcacccag accgcgctga tgaccatcga
     3061 gggcgagcgc gacgacatct ccggcgtggg ccagaccgaa gccgcgcagg atctgtgcag
     3121 cgccctggca ccggaaaaga agcttcacca ccttcaggaa ggcgtcggcc actacggcgt
     3181 cttcaatggc tcgcgcttcc gccgcgagat cgttccgcgt attgtcgagt tcgtgaacgc
     3241 caacgcctga tggcagggct cttcgcgcga ttccgcaaga cgccggcccc aaggccgctc
     3301 gaaccgggca tgatgaccat cggcctcgat caggttcccg tgcaattcag gcgccatgcc
     3361 caggcacgcc ggctggtgct gcggctctcg cccgacggct ccacggtcgt ggtgaccgtc
     3421 ccgccccgcg tcagccgggc cgaggccatg agcttcgccg agaaatcgcg cgactgggtg
     3481 gagaagcggc tgaaggcgca tggcggcggc atcgcactgg cgccgggaag ccgcattccg
     3541 ctgcgcggcg tcctgcacga gatccgccat gtaccggcgc gccgcggcac ggttgcggtc
     3601 gatgccaccg aggcactgat cttggttccc ggcgagctgc cgcatctgcc gcggcggctg
     3661 gtggactgga tgaagacggc ggcccgcgcc gacctacagc tggcgtcgcg caagtatgcg
     3721 gccctgatgg atgtggcgtt caagcgcatc accattcgcg atcaacgcag ccgctggggt
     3781 tcctgttcgg cgggcggcga attatcctac tcttggcgcc tgatcctcac gcccccgcat
     3841 gtgctcgact atgtggctgc tcatgaagtc gcgcatctca agcacatgaa ccacggcccg
//
LOCUS       DBNGVR010000059         2277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2377465, whole genome shotgun sequence.
ACCESSION   DBNGVR010000059 DBNGVR010000000
VERSION     DBNGVR010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2377465"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="AC16A0_01215"
     CDS             complement(<1..658)
                     /locus_tag="AC16A0_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3103975.1"
                     /translation="MRNSSQFISKRTSRIACTAATAASSSSKRRASPPVNAALPAEGP
                     HAEDLDLLIGQAARCREILARLANHDAEADEIYQRTKLLVMIEDLIEPLRGSEVEIAV
                     TSSADDAGKAKSEEPIFRRNPAIAYGLGNLLENAVDFAGNKVEVDARWTPSSVSITVR
                     DDGPGFRQDVFDRLGDPFVTTRPGYGEESGGKGQHDGMGLGFFIAKTLLERSGAAVSL
                     A"
     gene            624..2216
                     /locus_tag="AC16A0_01220"
     CDS             624..2216
                     /locus_tag="AC16A0_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3103976.1"
                     /translation="MRFEINWDEFLNLSMVYDHRRGELREVLQTAFVHLFPWVSSVSQ
                     NEVHQVIAARIVIAAFALTTSWAIWASARRLSSPVAALFAVFVYWSFSYVMQHGLALR
                     TDTLAAAPIMVALWLVLTRGDQKRMVVLAGLALGLAGAMTIKAIFFVPTLAIIAFLRA
                     AEGRDWRSGVVSVMLAGVASIAAFAAVIGLHVLTFDTFASPIAFLGRTTQATLSDTNR
                     SVFLTYAAYSVIENPGFYLLLIVTVVAVVRMMTVRVRRVQGAIALALLLPLLIPFFYR
                     DRVPYFYAMVLPPIVVAMSMGFEVILRQPSWRNLVALMALFIVAWGANLYAAFSQTNA
                     HQAAILRAIHDRFPAGSHYIDGRTMVSSFQKEGLFMSGWGMSDYRAAGVPVMAEVLRN
                     TPPAFLLANNRALYLDEFTPQESEASPVGLLAADVRTLQDNFVPFWGPIWVPGHRIAA
                     GDAGIETFAAGTYINQGPGTVEIAGRMVQPGGTIVLDQGVHPVVAGANATLSLAIEAP
                     AESPPARPIFSGFLWRYLSMPE"
BASE COUNT          362 a          728 c          705 g          482 t
ORIGIN      
        1 ttgccagcga cacggcagcg cccgagcgct caagcagcgt cttggcgatg aagaagccaa
       61 gccccatgcc gtcatgctgg ccctttccgc cgctctcctc gccatagccg ggccgcgtgg
      121 tcacgaaagg atcgcccagc cggtcgaaga cgtcctgcct gaaaccggga ccatcgtcgc
      181 ggaccgtgat ggagaccgac gacggcgtcc atcttgcatc gacctccacc ttgtttccgg
      241 cgaagtcgac ggcattctcc agcaggttgc cgagcccgta ggcaatggcc gggttgcgcc
      301 ggaagatggg ctcctccgac ttggccttcc cggcatcgtc ggcggaactc gtcaccgcga
      361 tctcgacttc ggaaccgcgc agcggctcga tcagatcctc gatcatcacc agcagcttgg
      421 tgcgctgata gatctcgtcg gcctcggcat cgtgattggc aaggcgcgca agaatctcgc
      481 ggcagcgcgc agcctggccg atcagcagat cgagatcctc cgcatgcgga ccttcggcgg
      541 gcagggccgc gttgaccggc gggctggccc gccttttgct gctgctgctc gctgccgtgg
      601 cagcggtaca agctattctg ctggtgcgct tcgagataaa ctgggacgag ttcctcaacc
      661 tgtcgatggt ctacgatcat cgccggggcg aattgcggga agtgctgcag acggcattcg
      721 ttcatctctt tccgtgggtc tcttccgtct cgcagaatga agtccatcag gtgatcgccg
      781 cccgcattgt gatcgccgcc tttgcgctca ccacctcctg ggccatctgg gcctctgccc
      841 gtcggctgtc cagccccgtg gctgccctgt tcgccgtctt cgtctactgg agcttctcct
      901 atgtgatgca gcacgggctg gcactcagga ccgacacgct ggccgcggcc cccatcatgg
      961 tcgcgctgtg gctggtgctg acccgtggcg accagaagcg gatggtcgtt ctggccggcc
     1021 ttgcccttgg gcttgcaggg gcgatgacga tcaaggcgat ctttttcgtt ccaacccttg
     1081 ccatcatcgc cttcctgcgc gcggccgagg ggcgtgactg gcgatccggg gtcgtcagcg
     1141 tcatgctggc tggcgttgcg tccattgctg cctttgcggc ggtgatcggc ctgcacgtcc
     1201 tgacctttga caccttcgca agtccgatcg cctttctggg gcgcaccacg caggcaacgc
     1261 tctccgacac gaaccggtcg gtcttcctta cctatgctgc ttattcggtg atcgagaacc
     1321 cgggattcta tttgctgctg attgtgacag tcgttgcagt cgtgcggatg atgacggtaa
     1381 gggtgcgaag ggtccaagga gcaattgccc ttgcgctcct gctgccgctg cttatcccct
     1441 tcttctatcg cgacagggtc ccttacttct atgcgatggt tctgccaccg atcgtggtgg
     1501 ccatgtcgat gggattcgag gtcattctgc gtcagccatc atggcgtaac ctggttgcgc
     1561 tcatggcgct cttcatcgtt gcatggggcg caaatctcta cgccgcgttc agtcagacca
     1621 atgcccacca ggcggcgatc ctccgcgcga tccatgaccg ctttccggcg ggcagtcact
     1681 acattgacgg ccgtaccatg gtgtcgtcct tccagaagga gggactcttc atgagcggat
     1741 ggggaatgag cgactatcgc gccgccggag tccccgtgat ggccgaggtc ctgcggaaca
     1801 cgccaccggc attcctgctg gcaaacaatc gcgctctgta tcttgatgag ttcacgccac
     1861 aagaatccga agcaagtccg gttggactgc ttgccgcgga tgtccggacc ctgcaggaca
     1921 atttcgttcc gttctggggg ccgatctggg tgccgggcca caggatcgca gccggagatg
     1981 ccggcatcga gaccttcgct gccggaacct acatcaatca ggggccgggc acagtggaga
     2041 tcgcgggtcg catggtccag ccgggcggca ccatcgtctt ggaccagggt gtgcatcctg
     2101 ttgtcgccgg tgccaatgcc acgctctcct tggcgatcga ggcaccggct gaatcgccgc
     2161 cggcacggcc gatctttagc ggattcctgt ggcggtatct gagcatgccg gagtgaccgg
     2221 atcaggcagg cggaaggacc cgcatgaccg atcgccgaga gtcctgcgca gccggtg
//
LOCUS       DBNGVR010000060         2148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_20541, whole genome shotgun sequence.
ACCESSION   DBNGVR010000060 DBNGVR010000000
VERSION     DBNGVR010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_20541"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="AC16A0_01225"
     CDS             <1..741
                     /locus_tag="AC16A0_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR3103977.1"
                     /translation="DALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTQDLKTFFPQV
                     PVTFDLSSPWVVVGLLFGGLLPYLFGGMSMTAVGRAAQSVVVEVRRQFKEKPGIMEGK
                     EKPDYARAVDLLTKAAIKEMILPSLLPVLSPIVVYFVINAIAGQAAAFSAVGAMLMGV
                     IVTGLFVAISMTAGGGAWDNAKKSFEDGFVDSKGVKHFKGSEAHKASVTGDTVGDPYK
                     DTAGPAVNPMIKITNIVALLLLAVLAGH"
     gene            complement(813..1802)
                     /locus_tag="AC16A0_01230"
     CDS             complement(813..1802)
                     /locus_tag="AC16A0_01230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3103978.1"
                     /translation="MDDSAYGTDDRRGNWMPTAPISYGPAFQWPPRPAELFKWFFGFP
                     GYLLPGNTLYAIGAVLIWTYLTPSLEDMRNFEPGWVGLILLRNLVLALLVYSAWHLWL
                     YVWRKQETAFKYNRQWPRESSAFLFGNQTYDNMFHTLVSGVPIWTAYEVLLLWAYANG
                     FAPFISFADHPVWFIAMFFVVPFIHEVGFYFAHRLLHFSPLYEVAHKLHHRNVNPGPW
                     SGLSMHPLEHLIYFSTILLFFLIPAHPVHMINLASRLGLAPAQGHTGFDRLALGGTAS
                     MDVSYYAHYLHHKYFEVNYADGMVPLDKWFGSFHDGTPEAHEAMKKRRQMRGT"
     gene            1938..>2148
                     /locus_tag="AC16A0_01235"
     CDS             1938..>2148
                     /locus_tag="AC16A0_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3103979.1"
                     /translation="MLIGIDWGGTKIEGVAMEPDGRELLRLREPTPRHDYHGCIAAIR
                     AMIAALEAKTGRTGSIGIGIPGSLEP"
BASE COUNT          410 a          644 c          719 g          375 t
ORIGIN      
        1 gacgcgctcg acgcggtggg caacaccacc aaggccgtga ccaagggcta tgccatcgga
       61 tccgccggcc tcggtgcact ggtgctcttc gccgcctata cgcaggacct caagacgttc
      121 ttcccgcagg tgccggtcac cttcgatctg tccagcccct gggtcgtggt cggcctgctc
      181 ttcggcggcc tgctgcccta cctcttcggc ggcatgtcga tgaccgccgt gggccgcgcg
      241 gcgcagtctg tggtggtcga ggttcggcgc cagttcaagg agaagccggg catcatggaa
      301 ggcaaggaga agccggacta tgcccgcgcc gtcgacctgc tgaccaaggc ggcgatcaag
      361 gaaatgatcc tgccctcgct gctgccggtc ctgtcgccca tcgtggtcta cttcgtgatc
      421 aacgccattg ccggccaggc cgcggccttc tcggcggtgg gcgccatgct gatgggcgtg
      481 atcgtgaccg gcctcttcgt cgccatctca atgacggcag gtggcggcgc ctgggacaat
      541 gcgaagaagt ccttcgagga cggcttcgtc gactccaagg gcgtcaagca cttcaagggc
      601 tccgaggcgc acaaggcctc ggtcactggc gacactgtcg gcgatcccta caaggacacc
      661 gccggtccgg cagtgaaccc gatgatcaag atcaccaaca tcgtggcgct gctgctgctg
      721 gcggttctcg ccgggcactg agcccggcag cgatgtgatg caacccaccc cgtccggagc
      781 gatccgggcg gggtttctct ttggcgcagc cgctaggttc cgcgcatctg gcggcgcttc
      841 ttcatcgcct catgcgcttc cggcgtgccg tcgtggaagg agccgaacca cttgtcgagc
      901 ggcaccatgc cgtcggcgta gttcacttcg aagtatttgt gatgcaggta gtgcgcatag
      961 tacgagacgt ccatcgacgc cgtgccgccc agcgccagcc ggtcgaagcc ggtgtgaccc
     1021 tgtgccggag caaggccaag ccgcgatgcc aggttgatca tgtgcacggg gtgggccggg
     1081 atgaggaaga acagcaggat cgtcgagaaa tagatcaggt gttcgagcgg gtgcatcgaa
     1141 agccccgacc acggccccgg gttgacattg cggtgatgca gcttgtgggc cacttcgtag
     1201 agcggcgaaa agtgaaggag ccggtgggcg aagtagaatc cgacctcgtg gatgaagggc
     1261 accacgaaga acatggcgat gaaccagacg ggatgatccg cgaaactgat gaagggcgcg
     1321 aagccgttgg cataggccca gagcagcagg acttcatagg cggtccagat cggcacgccg
     1381 ctcacgagcg tgtggaacat attgtcgtag gtctggttgc cgaaaaggaa ggccgatgac
     1441 tcgcgcggcc actggcgatt gtacttgaag gccgtctcct gtttccgcca gacatagagc
     1501 cacagatgcc aggcactgta gaccagaagg gccagcacca ggttgcgcag aaggatgagg
     1561 ccgacccagc ccggttcgaa gttgcgcatg tcctcgagcg aaggcgtcag ataggtccag
     1621 atgagcacag cgccgatggc gtagagcgtg ttgccgggca gcaggtatcc gggaaagccg
     1681 aagaaccact tgaacaattc ggcgggccgc ggcggccact gaaaggccgg accatagctg
     1741 atgggcgccg tgggcatcca attgccgcgc ctgtcatccg tcccataggc cgagtcatcc
     1801 atctgcagat cctccgcgcg gcatcgcacg gattattgca ctctcgactg cgccggacaa
     1861 tcttgtcgac tggtccgacc agatgcttgc attctggtgg cgggtgctgc cagtaagggc
     1921 cagcaatggg gttgagcatg ctgatcggaa tcgactgggg cgggaccaag atcgaaggtg
     1981 ttgcgatgga accggatggt cgggagctgc tccggcttcg cgaaccgacg ccgcggcacg
     2041 actaccacgg ctgcattgcg gccatcaggg cgatgatcgc cgcgctggag gcgaagaccg
     2101 gacgaacggg gtcgatcggc attggcattc caggctcgct cgagccca
//
LOCUS       DBNGVR010000061         2240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_414261, whole genome shotgun sequence.
ACCESSION   DBNGVR010000061 DBNGVR010000000
VERSION     DBNGVR010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_414261"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="AC16A0_01240"
     CDS             <1..137
                     /locus_tag="AC16A0_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR3103980.1"
                     /translation="RRLEQMRALDNGMRIVVVRVDTVPLGVDTPAELETARKMLRKTA
                     "
     gene            134..1003
                     /locus_tag="AC16A0_01245"
     CDS             134..1003
                     /locus_tag="AC16A0_01245"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3103981.1"
                     /translation="MTLKVAYQGEPGANSHIACLEVYPDCEPLSCGTFEDAFQAVKDG
                     EAQLAMIPIDNSVAGRVADIHHLLPRSNLYIVGEYFLPVHHQLMLAPDADLSTVRTVH
                     SHVHALGQCRKIMRELNLKPVVAADTAGAARELSQSLDSTRAAIATRLAAEIYGLKVV
                     RENIEDEAHNTTRFVILSPTPQDAEPEDQPVITSFVFRVRNVPAALYKAMGGFATNGV
                     NMTKLESYQLEGQFSATQFYADIEGHPADRNVRLALEELEFFSSYLRILGVYKAAPLR
                     AEIARRMEMPQSS"
     gene            complement(991..1848)
                     /gene="nudC"
                     /locus_tag="AC16A0_01250"
     CDS             complement(991..1848)
                     /gene="nudC"
                     /locus_tag="AC16A0_01250"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR3103982.1"
                     /translation="MALAYIGNRLDRCANRRSDAAWVETQRRAPDAKMVHINGEGTLA
                     AGGHLVTDRPAGDAALVVFLGRDAGGAPWFACPSTLTENLAPLRSLAMENLVPAEELG
                     IAAQARALIQWHERRGFCSNCGGRNRVVDGGYRRHCDSCGMDHFPRTDPVIIMVVRHG
                     SRILLGRQAAWTAGMYSALAGFLEPGETIEDAARREVLEEAGITVGTVRYAASQPWPF
                     PSSLMIGLIGEALTDRISLDDKELEDARWFDRAEALMMLDRTHPQGLWATNPYAIAHE
                     LVRLALQED"
     gene            complement(1929..>2240)
                     /locus_tag="AC16A0_01255"
     CDS             complement(1929..>2240)
                     /locus_tag="AC16A0_01255"
                     /inference="COORDINATES: protein motif:HMM:NF020839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HIR3103983.1"
                     /translation="GWTRLFSYRDRARLQLILRGKKVGFALEEIKEMLDLYNLRDGQL
                     TQLRIASTKMRERLEALRKQRVELEDAIADLERTVVVVDGMLKERETAGNAPKSAASA
                     S"
BASE COUNT          383 a          764 c          707 g          386 t
ORIGIN      
        1 gccgcaggct cgagcagatg cgcgcgctcg acaatggcat gcggatcgtt gtggtccggg
       61 tcgatacggt gccgctgggc gtcgacacgc ctgccgaact cgaaaccgcc cgcaagatgc
      121 tgaggaagac cgcatgactc tcaaagtcgc ctatcagggc gaacctggtg ccaactccca
      181 cattgcctgc ctcgaggtct atcccgactg cgagccgctc tcctgcggca ccttcgagga
      241 tgccttccag gcggtgaagg atggcgaggc gcagctggcg atgatcccca tcgacaattc
      301 ggtggcgggc cgggttgccg atatccatca cctcttgccg cggtccaatc tctacatcgt
      361 cggcgagtac ttcctgcccg tgcatcatca gctcatgctg gcgcctgacg ccgatctctc
      421 gaccgtcagg accgtgcaca gccacgttca tgccctcggg cagtgccgca agatcatgcg
      481 cgaactcaac ctcaagccgg ttgtcgccgc cgacacggca ggggccgccc gcgagctttc
      541 gcagtccctc gattccaccc gcgcggccat cgccacccgg ctggcggccg agatctatgg
      601 cctcaaggtg gtgcgcgaaa acatcgagga cgaggcgcac aacaccaccc gcttcgtcat
      661 cctctcgccc acgccgcagg atgccgagcc ggaagaccag ccggtgatca ccagctttgt
      721 cttccgtgta cggaatgttc cggcggcgct ctacaaggcc atgggcggat tcgccaccaa
      781 cggcgtgaac atgaccaagc tcgaatccta tcagctcgag ggccagttct cggcgaccca
      841 gttctatgcc gacatcgagg gccacccggc cgatcgcaac gtccgcctgg ccctggaaga
      901 gctcgagttc ttctcaagct acctgcgcat tctcggcgtc tacaaggccg cgccgctgcg
      961 cgccgagatc gcgcgccgca tggagatgcc tcaatcctcc tgaagcgcca ggcgcaccag
     1021 ttcatgcgcg atggcatagg gattggtggc ccagagcccc tgcggatgcg tgcggtccag
     1081 catcatcagg gcttccgcgc ggtcgaacca gcgcgcatcc tccagctcct tgtcgtccag
     1141 gctgatccgg tcggtcagcg cttcgccgat caggccgatc atcaggcttg acggaaaggg
     1201 ccagggctgg cttgcggcat agcggactgt gccgaccgtg atgcccgcct cttccagcac
     1261 ttcgcgccgg gccgcgtcct cgatcgtctc gccgggttcg aggaagcccg ccagcgccga
     1321 atacatgccg gcagtccagg ccgcctggcg gcccagaagg atgcgcgagc catgtcgcac
     1381 caccatgatg atgacggggt ccgtccgcgg aaagtgatcc atgccgcagc tgtcgcagtg
     1441 gcggcggtag ccgccgtcga caacgcggtt tcgcccgccg cagttggagc agaaaccgcg
     1501 gcgctcatgc cactggatga gtgcgcgggc ctgggccgcg atgccgagtt cctcggcggg
     1561 caccaggttc tccatggcca ggctgcgcag gggcgcgagg ttctcggtca gggttgacgg
     1621 gcaggcgaac cacggcgcac ccccagcgtc acgtccgaga aagacgacca gagccgcatc
     1681 gccggcgggc cggtcagtga cgagatgccc tccggcagcc agcgttccct cgccgttgat
     1741 gtgaaccatc ttcgcatccg gcgcgcgccg ctgggtttcc acccacgccg cgtcggagcg
     1801 ccggttggcg caacggtcga gccggttgcc gatgtaagct agtgccattc cggaagggat
     1861 cctcgcaaag caaaatcgcc cgggcgacta ttcacccgga cgacggtatc ggcaatatgt
     1921 tgcggtggtc agctggcgct ggcggcactc ttgggcgcgt tgccggcggt ctcgcgttcc
     1981 ttgagcatgc cgtcgacaac cacgacggta cgctccagat cggcgatggc atcttcaagc
     2041 tcgacccgct gcttgcgcag ggcctcgagg cgttcgcgca tcttggtgga ggcgatccgc
     2101 agctgggtca gctggccgtc gcgaagattg taaagatcga gcatctcctt gatctcttcc
     2161 agcgcgaagc cgaccttctt tccgcgcagg atcagctgca gccgggcgcg atcacggtag
     2221 ctgaacagcc gggtccagcc
//
LOCUS       DBNGVR010000062         4230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1592990, whole genome shotgun sequence.
ACCESSION   DBNGVR010000062 DBNGVR010000000
VERSION     DBNGVR010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1592990"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="AC16A0_01260"
     CDS             complement(<1..524)
                     /locus_tag="AC16A0_01260"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIR3103984.1"
                     /translation="MKKLINDVSTVLTESLDGFAAAHADLVTLGAEHKFVRRKVLKPG
                     KVALLSGGGAGHEPLHAGFVGHGMLDAACPGQVFTSPTPDQMMAAADAVDTGAGLLFI
                     VKNYEGDVMNFDMAREMCGREVETVITDDDVAVENSLYTTGRRGVAGTVLVERLVGAA
                     AERGEGLADLKALG"
     gene            complement(521..949)
                     /locus_tag="AC16A0_01265"
     CDS             complement(521..949)
                     /locus_tag="AC16A0_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103985.1"
                     /translation="MGTAQLQSVAKYKALLAGIIERRPSGTRQRLAAALSKNRSFISQ
                     ITNPAYATPVPASHLDVIFDVCHFSPVEKRQFLEAYGLAHPKRLAARPEARTAAGPAT
                     VTLPDLGSEARNKKLQALVQDFVQHMVALIDQEPTKGRRS"
     gene            complement(951..2537)
                     /gene="ptsP"
                     /locus_tag="AC16A0_01270"
     CDS             complement(951..2537)
                     /gene="ptsP"
                     /locus_tag="AC16A0_01270"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR3103986.1"
                     /translation="MAPREYRGHCASDGIFAGPILRIDTALSRTRSVGTSEAEAGALT
                     ASIETAMAEIVALMEKTSGDAADILAFQLAMLEDDALRAPAFEAIAGGTPAHEAWSAA
                     LGGEILGYESSDDEYFRARAEDLRDIRDRVLRHLFGVGPEAAGGAAVYFGEDMPPTRF
                     LGIDWSQGGAIALSRGSSTSHVAMLARARGIPMVVGLGAADPAAGSAAIVDGGEALLL
                     LDPDAAMAERLARRHADHAAQQARDSSFLLKPAALKDGTRIQVTINVAGVDELDHIDP
                     ACCDGIGLMRSEFLFRDGAPLPDEDEQYRAYRRFVEWAGGRPVTIRTLDIGGDKPVRG
                     LTPEGEANPFLGLRGVRLTLARPDVFRTQLRALARAAAHGDLKVMIPMVSIPAELAQV
                     AALLDAVMEDLLHDGVACRKPPLGIMVEVPAVAVAPDLFRDAAFFSIGSNDLTQYVTA
                     TARDGTMVEGLGRADHPAVMRLIAQVAQFGHEAGIPVSLCGDMASEPAHLSALIGAGL
                     RALSVAPARVARVKAALAEL"
     gene            complement(2524..2820)
                     /locus_tag="AC16A0_01275"
     CDS             complement(2524..2820)
                     /locus_tag="AC16A0_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3103987.1"
                     /translation="MSEAQSRVLITHDVGLHARPSVKLTKLAKGFQSRIEIAGSEAGP
                     WIDAKSIVKVMAMKAKQNTELFVRASGDDAAAAVAAIVDLVTRDFDEAGDHGAA"
     gene            complement(2817..3209)
                     /gene="dhaM"
                     /locus_tag="AC16A0_01280"
     CDS             complement(2817..3209)
                     /gene="dhaM"
                     /locus_tag="AC16A0_01280"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HIR3103988.1"
                     /translation="MSGKVGIVIVSHSPDVAKGAADMVRQMVGNDVPLGFCGGNPDGG
                     LGTSVEKILAAIDQAWSEAGVAILVDLGGAETNSEMAIEMLPEDRQGKVVICNAPVVE
                     GAVMAATESWGGSSLAEVKKTAEELSPQ"
     gene            complement(3206..3808)
                     /gene="dhaL"
                     /locus_tag="AC16A0_01285"
     CDS             complement(3206..3808)
                     /gene="dhaL"
                     /locus_tag="AC16A0_01285"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HIR3103989.1"
                     /translation="MAPPLDRLIDTLAQRIIAASEELTALDSAIGDADHGLNMKRGFE
                     AILAERANLVAKPLPDALKAMGTILVMKVGGASGPLYGTLFMTLGKELPADPSVADAA
                     RAMAAAMAALKARGKADLGNKTMLDVLGPLADHLGSGGASYASIRKLAAESAAATVPM
                     KAIRGRASFLGERSIGHMDPGARSSQIMIDAVCDVLEGEA"
     gene            3969..>4230
                     /locus_tag="AC16A0_01290"
     CDS             3969..>4230
                     /locus_tag="AC16A0_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011649355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HIR3103990.1"
                     /translation="MPRFDRKTLMEKFQDMKRRRVPIVGGGAGTGLSAKCEEEGGIDL
                     IVIYNSGRYRMAGRGSLSGILAYGNANEIVMEMAREVLPVTKK"
BASE COUNT          684 a         1434 c         1409 g          703 t
ORIGIN      
        1 tcgcccagcg ccttcaggtc ggccaggccc tcgccgcgct cggccgccgc accgacgagg
       61 cgctcgacca gcaccgtccc cgccacgccg cggcgcccgg tggtgtagag cgaattctcg
      121 accgccacgt cgtcgtcggt gatcacggtc tccacctcgc ggccgcacat ctcgcgcgcc
      181 atgtcgaagt tcatcacgtc gccctcgtag ttcttcacga tgaagaggag gccggcgccc
      241 gtatccaccg catcggcggc cgccatcatc tggtcgggcg tgggcgaggt gaagacctga
      301 cccgggcagg cggcatccag catgccatgg cccacgaagc ccgcatgcag cggctcatgg
      361 ccggcaccac cgcccgagag cagcgccacc ttgccgggct tcagcacctt gcgccgcacg
      421 aatttgtgct ccgctccgag ggtgacgaga tcggcatggg ccgcggcgaa gccgtcgagg
      481 ctctcggtca ggacggtcga aacatcgttg atgagcttct tcatgatcgt ctccctttgg
      541 tcggttcctg gtcgatcagc gccaccatgt gctggacgaa gtcctgcacc agggcctgga
      601 gtttcttgtt gcgggcctcg ctgccgaggt ccggcagcgt gacggttgcg ggtcctgccg
      661 ctgtccgggc ctcgggccgg gcggccagcc gcttcggatg cgccaggcca taggcctcga
      721 gaaactgccg cttctcgacc ggcgaaaagt ggcagacatc gaagatcacg tcgagatgcg
      781 aggcgggcac cggcgtggca taggcgggat tggtgatctg cgaaatgaag ctgcggttct
      841 tggacagtgc ggcggcaaga cgctggcgcg tgccggacgg ccgccgttcg atgatacccg
      901 ccagcagcgc cttgtacttc gcaaccgatt gcagctgcgc ggtacccatc ctagagctcc
      961 gccagcgcgg ccttgacccg cgccacccgg gccggcgcca ccgagagcgc gcgcagcccg
     1021 gctccgatca gggcggaaag atgggcgggt tcgctcgcca tgtcgccgca gaggctgacc
     1081 ggaatgccgg cctcgtggcc aaactgcgcc acctgggcga tgagccgcat caccgccgga
     1141 tgatcggccc ggcccaggcc ttccaccatc gtgccatcac gcgcggtcgc cgtcacgtat
     1201 tgcgtgaggt cattggaacc gatggaaaag aaggccgcgt cgcgaaacag gtcgggcgcc
     1261 acggcgacgg cgggcacttc caccatgatg ccgagcggtg gcttgcggca ggcgacgccg
     1321 tcgtgcagca ggtcttccat caccgcgtcg agcagtgcgg caacctgtgc cagttccgcg
     1381 gggatgctca ccatcgggat catcaccttg agatcgccat gcgccgcggc gcgggccagg
     1441 gcgcgcagct gcgtgcggaa gacatccggc cgcgccaggg tgaggcgcac gccgcgcagg
     1501 ccgaggaagg gattggcttc gccctcgggc gtgaggccgc gcaccggctt gtcgccgcca
     1561 atgtcgaggg tgcggatggt gacgggccgt ccgcccgccc actccacgaa gcggcggtag
     1621 gcgcgatact gctcgtcctc gtccggcaat ggcgcgccgt cgcggaacag gaactccgag
     1681 cgcatcaggc cgatgccatc gcagcaggcg ggatcgatgt gatcgagctc gtccacgccg
     1741 gcaacattga tcgtgacctg gatgcgggtg ccatccttca gcgcggcggg cttcagcagg
     1801 aaggaactgt cgcgtgcctg ttgtgcagca tgatctgcgt ggcgccgggc caggcgctct
     1861 gccatcgccg catcgggatc gaggagcaga agtgcttctc cgccatcgac gatggccgcg
     1921 gaacctgccg ccggatcggc ggcgccaagt cccaccacca tgggaatgcc tcgtgccctg
     1981 gccagcatgg ccacatggct ggtgctgctg ccgcggctca gcgcgatggc gccgccctgc
     2041 gaccagtcga tgccgagaaa gcgggtcggc ggcatgtctt cgccgaaata gaccgcagcg
     2101 cccccggccg cctccgggcc tacgccgaag agatgccgca gcacccggtc gcggatgtcg
     2161 cggagatctt ccgcgcgggc gcggaagtat tcgtcgtcgg aggactcgta gcccaggatc
     2221 tcgccgccca gtgccgcaga ccaggcctca tgggcaggcg ttccgccggc gatagcctcg
     2281 aatgccggcg cgcgcagcgc atcgtcttcc agcatggcaa gctggaaggc gagaatgtct
     2341 gcggcatcgc cgctggtctt ctccatcagc gccacgatct ccgccatggc cgtttcgatg
     2401 gatgctgtca gcgcccccgc ctccgcctcc gatgttccca cactgcgcgt tcgcgacagt
     2461 gccgtatcga tgcgcaggat gggaccggcg aagatgccgt cggaggcgca atggccccgg
     2521 tactcacgcg gcgccatggt ctcctgcctc gtcgaagtcg cgcgtcacca ggtcgacgat
     2581 ggcggcaacc gccgcggccg catcgtcgcc gctggcgcgc acgaacagct cggtgttctg
     2641 cttcgccttc atcgccatga ccttgacgat gctcttggca tcgatccacg gaccggcttc
     2701 gctgccggca atctcgatac ggctttgaaa ccccttggcc agcttggtga gcttcaccga
     2761 aggccgtgca tgcaggccca cgtcatgggt gatgagaacg cgggattggg cttcgctcat
     2821 tgcggtgaca gctcctcggc cgtcttcttt acttcggcca gggaggaccc gccccaggac
     2881 tcggtggcgg ccatcacggc accttccacc accggggcat tgcagatcac caccttgccc
     2941 tgccggtctt cggggagcat ctcgatggcc atctcgctgt tggtctccgc tccgccgaga
     3001 tcgacgagaa tggcaacgcc ggcctccgac caggcctggt cgatggcggc aaggattttc
     3061 tccacgctgg tgccgagacc gccatcggga ttgccgccgc agaagcccag cggcacgtca
     3121 ttgcccacca tctggcgcac catgtcggcc gcgcccttgg ccacgtccgg cgaatgcgag
     3181 acgatgacaa ttccaacttt tccgctcatg cctcgccctc cagcacgtcg cagaccgcat
     3241 cgatcatgat ctggctcgag cgcgcacccg ggtccatgtg accgatggag cgctcgccga
     3301 ggaaggaggc gcggccgcgg atcgccttca ttggcaccgt ggcggcggcg ctctcggcgg
     3361 cgagcttgcg gatgctcgcg tagctggcgc cgccggagcc gagatgatcg gcgagcggac
     3421 ccagcacgtc gagcatcgtc ttgttgccga gatcggcctt gccgcgcgcc ttgagcgccg
     3481 ccatcgccgc ggccatggcc ctggccgcgt cggccaccga aggatcggcg ggcagttcct
     3541 tgcccagcgt catgaagagc gtgccataga gcgggcccga ggcgccgccc accttcatca
     3601 ccaggatggt gcccatggcc ttcagcgcat ctggcagcgg cttggccacc agattggcgc
     3661 gctccgccag gattgcctcg aagccgcgct tcatgttgag cccgtggtcg gcatcgccga
     3721 tggcggaatc aagcgcagtc agttcttcgg acgcggcgat aatgcgctgg gccagcgtgt
     3781 cgatgagcct gtcgagggga ggcgccacgg cggaaatcct catgtttagc gattgtgttt
     3841 agcaaaacag gatttactaa acatggcaag agggtcgctg tgcagctgcg agatcgcgtg
     3901 attttgcgcg cgacagcggc ggcggatcga ctagattgac gccgcgattt gccagaggac
     3961 gttcccgcat gccccgtttc gaccgcaaga cactgatgga gaagttccag gacatgaagc
     4021 gccgccgcgt gcccatcgtg ggcggcgggg cgggcaccgg cctctccgcc aaatgcgagg
     4081 aggagggcgg catcgacctc atcgtcatct acaattccgg ccgctaccgc atggccggcc
     4141 gcggctcgct ctccggcatc ctggcctatg gcaacgccaa tgagatcgta atggagatgg
     4201 cgcgggaggt tctgcccgtg acgaagaaga
//
LOCUS       DBNGVR010000063         6025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2968626, whole genome shotgun sequence.
ACCESSION   DBNGVR010000063 DBNGVR010000000
VERSION     DBNGVR010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2968626"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16A0_01295"
     CDS             <1..237
                     /locus_tag="AC16A0_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103991.1"
                     /translation="FGDKRDYWEIAYRRTIEEGKPTQGIIRGPRVNKEHLVQYWLKLP
                     LARAGGDGVGMILCLDHFIPAVDDEEAGGRALGT"
     gene            316..909
                     /locus_tag="AC16A0_01300"
     CDS             316..909
                     /locus_tag="AC16A0_01300"
                     /inference="COORDINATES: protein motif:HMM:NF040428.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HIR3103992.1"
                     /translation="MPKHPPIARQDQTLTGHRPPRPRDEGGPNGQPARNDAESPLAWL
                     RARRGRTGEALISDSQFMAGEKLRRDFERAHFQGRVTTNWDLSATAGRSAGNAVAELS
                     DGALAARERYHRAIAAVGPELAGILVPVCCLAAGLEQAERSLNLPQRSGKAILALALT
                     ALARHYGIRGDSSPRAARARNWGLPDYRPALLTPDES"
     gene            complement(873..1322)
                     /locus_tag="AC16A0_01305"
     CDS             complement(873..1322)
                     /locus_tag="AC16A0_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HIR3103993.1"
                     /translation="MARIDELLSDFEVLDDWEDRYRHVIELGRELKPLPEADHTAANK
                     VMGCASQVWITSSASGQAADPVIDYVGDSDALIVKGLIAIAFMIYSGKRASEILATDA
                     APVLRQLGLSDHLTPQRSNGFASMIARIKSDAAAARQDSSGVSSAGR"
     gene            complement(1373..1786)
                     /locus_tag="AC16A0_01310"
     CDS             complement(1373..1786)
                     /locus_tag="AC16A0_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103994.1"
                     /translation="MRIVRTLLVLGGIAVFLPAPPGDHPTGSQEERPAAPQGLLATTA
                     LAVADLFDLCAEGDWTCDLAGRSAAHLESKVKYSAWLAYDWAVTPSPRARLEVGDAGD
                     GTSSSDSSSSMPNASTLRLDDMVPAWRGPAPQNKG"
     gene            2014..2664
                     /locus_tag="AC16A0_01315"
     CDS             2014..2664
                     /locus_tag="AC16A0_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3103995.1"
                     /translation="MRDEEEAEVRRWRPAKLFAIATSGLLSVAVSYNALFAQSGGHHL
                     AAHDNAARVEVDVPAGGNTIQLKYDPVVEALQRQLFSAGHYKGTIDGVLGKKTRSAIM
                     SYQTAVGMDATGEPSPELVEHIRFTREVAEASLFTGTVDAAPDAEARANVRRVQTGLA
                     ELAYGPGEINGELTEATRRAIRAFQHDRKMPETGEITPELLTELAKLSGQSELAAE"
     gene            2666..3007
                     /locus_tag="AC16A0_01320"
     CDS             2666..3007
                     /locus_tag="AC16A0_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HIR3103996.1"
                     /translation="MDRLKTAIWVSGLLRRCQAQGLFGAVIRKGAEEAGAVFVIVNHL
                     DGSFHLLGPAPGPTVDDDGERRWIVEAAPPASQAEIDAILGKRRRGDPDLWIVEIEDR
                     HGLAGLAATPA"
     gene            3087..3281
                     /locus_tag="AC16A0_01325"
     CDS             3087..3281
                     /locus_tag="AC16A0_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103997.1"
                     /translation="MTTLVPFPIEKARRSASGEKGPDAMIIIFPGVRIERMDFSLADR
                     LPTRGNRRTSQAQKFDYDSL"
     gene            complement(3317..3874)
                     /locus_tag="AC16A0_01330"
     CDS             complement(3317..3874)
                     /locus_tag="AC16A0_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3103998.1"
                     /translation="MGRLYWIAAAVLAAIAAHSAFILAAPALSFSRDVTALSSAAGPN
                     RFFILDAAQQAKLFPAYPATSVIGLCAFDVSDGIVTLTATMPDGYWTLNVYSRRGNVI
                     YAVNDSQAGTNTFTLELSLEPGILDMLLQATQKERPDIDIGWTVESPDSTGLAVLLYP
                     VSEPGARAAAIRRMESSACSARAAS"
     gene            complement(3874..4431)
                     /locus_tag="AC16A0_01335"
     CDS             complement(3874..4431)
                     /locus_tag="AC16A0_01335"
                     /inference="COORDINATES: protein motif:HMM:NF018452.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR3103999.1"
                     /translation="MQRKTSLIGGPVFLLAGAIAGLLSAYIASDALGTAPIRAGSPWM
                     ERKTTADSPAQPYAVAHFLLGGRLPPPPTQMTELTAALDDDGRRLTSACIIELTLPAG
                     PRPRWWSLGVLGHSGSLLTSDAAIAETDGTIRVSVAPTPRPGNWIAAPDNRGFELIYS
                     AAGSDGGAGFASGNVPLFTIKQAGC"
     gene            complement(4431..>6025)
                     /locus_tag="AC16A0_01340"
     CDS             complement(4431..>6025)
                     /locus_tag="AC16A0_01340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3104000.1"
                     /translation="AARARTNTVLYRMLDSGFITQGELIQARREPAQVVPQNTADSPD
                     WFLDWAYRDTLEVIEREKITGAYVLEVKTTIDIPLQSASQRLINEALIANGANYDVSQ
                     AASVTMAPDGAVRAIVGGASYEQSQFNRATDALRQSGSSFKPFVYLAALLAGYTPDQI
                     VVDGPVSVSDWSPKNYSGKYAGRTTLSNALAHSYNSIPVKLMIDIGRKAIIETSHRVG
                     IKGELENWAPMVLGTSALSLMDLSTGYATFANGGKLSQPYAVLEIRKANGDILYSREE
                     KAEMPRQVVPEEKIAELNSMLSAVVKTGTGRRADLGFAPQGGKTGTNQSYRDAWYIGF
                     TANNVTGVWFGNDDFTPMNEVTGGLLPAPVWKEIMLVAERNFEPRGLAGIPFDETYAL
                     AAAKMREEMAAAMPPAATDQQEIDDAAGMPVVAQSGDVSQVLKGMFNLFEGPNQPVAP
                     PKAKVQPRKTTLILPKANTSMGESLEETDTDMRSDKKKNIVQKIFGVIEDPFKKKEKK
                     KKKKDNDGDKDKKKKKKFLGIF"
BASE COUNT         1053 a         1915 c         1976 g         1081 t
ORIGIN      
        1 tttggcgaca agcgcgacta ttgggaaatc gcctatcgcc gcaccatcga agagggcaag
       61 cccacccagg gcatcattcg aggtccgcgc gtgaacaagg aacatctggt gcaatactgg
      121 ttgaaactgc cgttggcccg ggccggcggc gatggcgtcg gcatgatcct ctgcctcgac
      181 catttcattc cggcagtgga tgacgaagag gccgggggcc gcgctctcgg cacgtgaccg
      241 gaagatagtc cggaagatag gtattgattc cttgttccgg gtgggctagt catgtcctga
      301 cacaggagaa gacgcatgcc caagcaccct ccaatcgcca ggcaagacca aacactgaca
      361 ggtcatcgcc cgccgcgtcc gcgggacgaa ggcgggccaa acggacaacc cgcccggaat
      421 gacgccgaga gtccgctcgc ctggctgcgc gcgaggcggg ggaggacggg cgaggcgctt
      481 atcagtgaca gccagttcat ggccggcgag aagctccgcc gcgacttcga gcgcgcccat
      541 ttccagggac gggtcaccac caattgggat ctttcggcga ccgcaggccg gagcgccggc
      601 aatgccgttg ccgaactctc cgatggcgcc ctcgcggccc gggagcgcta tcaccgcgcc
      661 atcgcagcag tcggtcccga actggccggc attctcgtcc cggtctgctg cctcgcggca
      721 gggctggagc aggcggagcg gtcgctcaat ctgccgcagc ggagcggcaa agccattctc
      781 gccctggcgc tgacggccct ggcgcggcac tacggcatcc ggggcgactc ttcgccacgc
      841 gccgcaaggg ccaggaactg gggcttgccc gactatcgtc ccgcgctgct cacgcccgac
      901 gaatcctgac gcgccgctgc ggcatcggac ttgatgcggg cgatcatcga ggcgaagcca
      961 ttggaacgct ggggcgtcag gtggtcgctc aagcccagct ggcgcagcac gggtgccgcg
     1021 tcggtggcga ggatctcgct ggcccgcttg ccggaataga tcatgaaggc aatggcgatc
     1081 agccccttga cgatcagggc atcgctgtca ccgacatagt cgatcaccgg atccgcagcc
     1141 tgaccgctgg cggacgaggt gatccagacc tggctcgcgc agcccatgac cttgttggcg
     1201 gccgtgtggt cggcctcggg cagaggcttc agctcgcgtc ccagttcgat gacatggcga
     1261 tagcggtcct cccagtcgtc gaggacttcg aagtcggaaa gcagttcgtc aatgcgcgcc
     1321 acgccggtgt tcctcggttg ccggcccgac atagggcggc gggaggcgaa gctcaaccct
     1381 tgttctgggg cgcgggtccc cgccaggcgg gcaccatgtc gtcgagccgc agcgtcgagg
     1441 cattgggcat ggaagaggag gaatcgctgg atgatgtgcc atcgcctgcg tctccgacct
     1501 caagcctggc gcggggcgag ggcgtgaccg cccagtcata ggccagccag gcgctatact
     1561 tgaccttgct ttcgagatgg gcagcgctcc gcccggccag gtcgcaggtc cagtctcctt
     1621 cggcgcagag atcgaacaga tcggccacgg ccagggccgt ggtcgcgagc aggccttgcg
     1681 gcgctgcagg gcgctcctcc tgcgatccgg tggggtggtc gccaggcggc gcgggcagga
     1741 acaccgcaat gccgcccagg accaggagtg ttcgaacgat acgcatcccg tgtcctgtat
     1801 caggggcggc tgttctagca caccggcagt gcaggacgct caaaacgaag acgcgaattc
     1861 gcatcaaatt tgacggattt gcttaaccgg agcttaaccc tggcaagcca ccctccgccg
     1921 gaaaggcggc gttaacggta gctgttgcat tttggatgca gcgacgcggg ctgggtcccg
     1981 tccagccgga atgcttgggt ttgggtagag cggatgcgtg acgaggaaga ggcggaagtg
     2041 cggcggtggc ggccggcaaa gctctttgcc attgccacca gcggactgct gagcgtcgcc
     2101 gtctcctaca atgcactttt tgcccaaagc ggcggccacc acttggccgc tcacgacaac
     2161 gccgcccgcg tcgaggtcga cgttccggcc ggaggcaaca ccattcagct caagtacgat
     2221 ccggtcgtcg aggcgctcca gcgccagctc ttttctgcgg gtcactacaa gggaacgatc
     2281 gacggcgtcc tcggcaagaa gacccgcagc gccatcatgt cctatcagac cgccgtgggc
     2341 atggatgcca ccggcgaacc ctcgccggaa ctcgtcgagc acatccgctt cacccgcgaa
     2401 gttgccgagg cgtctctctt caccggcacg gtcgatgccg cgcctgatgc cgaggcccgt
     2461 gccaatgtcc gccgggtgca gaccgggctt gcggaacttg cctatggtcc gggcgagatc
     2521 aatggcgaac tgaccgaagc gacccgcagg gccatccgcg ccttccagca tgaccgcaaa
     2581 atgcccgaaa ccggcgagat tactccggaa ctgctgaccg agctggccaa gttgagcggc
     2641 cagtccgagc tggcagccga gtagattgga ccgactcaag accgcgatct gggtgagtgg
     2701 cctgttgcgg cgctgccagg cccagggcct cttcggtgcg gtgatccgca agggagcgga
     2761 ggaggcaggc gccgtcttcg tgatcgtcaa tcatcttgac ggcagctttc acctgcttgg
     2821 acccgcaccc gggccgactg tcgacgacga tggcgagcgg cgctggattg tcgaggccgc
     2881 gccgcctgcg tcgcaagcgg agatcgacgc catcctcggc aagcggcgcc gcggcgatcc
     2941 cgatctctgg atcgtcgaaa tcgaggaccg gcacggactc gcgggcctcg ccgctacacc
     3001 cgcctgacgc tcgggccagg cttaggcaat tgttaaccat accggtgcct aatcgcgcca
     3061 tcggcgaagc gagagagcgc actggcatga cgacccttgt tccatttccc atcgaaaagg
     3121 cccgcaggtc agcaagcggc gagaaggggc cggatgccat gatcatcatt ttcccgggcg
     3181 tgcgcatcga gcgtatggac ttcagcctgg cggaccggct gccgacgcgc ggcaaccgcc
     3241 gcacgtccca ggcgcagaag ttcgactacg acagtctctg accagccgcg ccgatgttcg
     3301 gcgcgttgtc aggcctctag gacgcggctc tggcgctaca ggcgctggac tccatccggc
     3361 ggatcgccgc ggcacgcgcg cctggttccg agacgggata gagcagcacc gcgaggccgg
     3421 tcgaatcggg cgactccacc gtccagccga tgtcgatgtc cggtctttcc ttctgcgtcg
     3481 cctgcaggag catgtcgagg atccccggtt ccagcgagag ctcgagcgta aaggtattgg
     3541 tgcccgcctg gctgtcgttc acggcataga tgacattgcc gcggcgggag tagacgttca
     3601 gggtccaata gccatcgggc atcgtggccg tcagggtcac gatgccgtcg gacacatcga
     3661 aggcgcagag tccgatgacg ctcgtggccg gataggccgg aaacagcttt gcctgctgtg
     3721 ctgcgtcaag aatgaagaag cggttgggac cggcggccga ggacaaggcg gtgacgtccc
     3781 ggctgaagga gagggccgga gcggcgagaa tgaaggccga gtgcgccgcg atggcggcga
     3841 gcacggcggc ggcgatccag taaaggcgtc ccatcagcag ccagcctgct tgatcgtgaa
     3901 gagcgggaca ttgcccgagg cgaagcccgc gccgccatcg ctgccggcgg cgctgtagat
     3961 cagttcgaaa ccgcgattgt cgggcgctgc gatccagttg ccgggccgcg gcgttggcgc
     4021 gacgctcacc cggatcgtgc catcggtctc ggcaatggcg gcgtcgcttg tcagaaggct
     4081 gccgctgtgg ccgagcacgc caagactcca ccagcgcggg cgcggcccgg caggcaaggt
     4141 caactcgatg atgcaggccg aagtcaagcg cctgccgtcg tcatccagcg ccgctgtcag
     4201 ttccgtcatc tgggtcggcg gaggcggcag gcggccgccc agcagaaaat gcgcaaccgc
     4261 gtagggctgg gcagggctgt cggccgtcgt ctttcgttcc atccacggcg agcctgcgcg
     4321 gatgggggcc gtgcccagcg cgtccgacgc gatataggca ctcagcaggc cggcaattgc
     4381 cccggccagc agaaacaccg gcccaccgat caggctagtt ttgcgctgca tcagaatatt
     4441 cccaggaact tctttttctt cttcttgtcc ttgtcaccgt cattgtcctt cttcttcttc
     4501 tttttctcct tcttcttgaa gggatcctcg atcacgccga agatcttctg gacaatgttc
     4561 ttcttcttgt ccgagcgcat gtcggtgtcg gtttcctcca ggctctcgcc catcgaggta
     4621 tttgccttgg gcaggatgag cgtggtcttg cgcggctgca ccttggcctt gggcggcgcc
     4681 accggctggt tcgggccttc aaagagattg aacatgccct tgagcacctg cgacacgtct
     4741 ccggactggg cgaccaccgg catgcctgcg gcgtcgtcga tctcctgctg gtcggtagcg
     4801 gccggcggca tggcggcagc catctcctcc cgcatcttgg cagcggccag cgcgtaggtt
     4861 tcatcaaagg gaatgcccgc aagcccccgg ggctcgaagt ttcgttcggc taccagcatg
     4921 atttccttcc ataccggcgc cggcagcaag ccccctgtca cctcgttcat cggcgtgaag
     4981 tcgtcattgc cgaaccagac gccggtgacg ttgttcgccg tgaagccaat gtaccaggca
     5041 tcgcggtagc tctggttggt gccggtcttt ccgccctgcg gggcaaaacc aaggtcagcc
     5101 cggcggccgg tgccggtctt gaccacggcg gacagcatcg aattcaactc ggcgatcttt
     5161 tcctccggca ccacctgacg cggcatttct gccttttcct cgcggctgta gagaatgtcg
     5221 ccattggcct tgcggatttc cagcacggca tagggttggg acagcttgcc gccattggcg
     5281 aacgtcgcat agccggtgga gaggtccatc agcgacaggg cgcttgtgcc cagaaccatc
     5341 ggagcccagt tctcgagttc gcccttgatg cccacccggt gcgatgtctc gatgatcgcc
     5401 ttgcggccga tatcgatcat cagcttcacg ggaatcgaat tgtaggaatg tgcgagggca
     5461 ttgctgagcg tcgtgcggcc ggcatatttc ccgctgtagt tctttggcga ccagtcggag
     5521 acagagaccg gtccatcgac gacgatctga tcaggtgtat agcctgccag cagcgccgcc
     5581 agatagacga agggcttgaa ggaagatccc gactgccgca gcgcatcggt ggcgcggttg
     5641 aactggctct gttcatagct ggcgccgccg acgatggccc tgaccgcgcc gtccggcgcc
     5701 atggtgacgg acgccgcctg gctcacgtca tagttcgccc cgttggcgat cagggcttcg
     5761 ttgatcaacc gctgcgacgc cgactgcaaa gggatatcga ttgttgtctt gacctcgaga
     5821 acataggcgc ccgtgatctt ctcccgttcg atgacttcga gcgtgtcgcg ataggcccag
     5881 tcgaggaacc agtccgggct gtcggcggtg ttctgcggca cgacctgcgc cggttcgcgc
     5941 cgagcctgaa tcaactctcc ctgggttatg aaacccgagt cgagcatgcg gtagagcacc
     6001 gtgttggtgc gggcgcgggc agctt
//
LOCUS       DBNGVR010000064         3244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2577110, whole genome shotgun sequence.
ACCESSION   DBNGVR010000064 DBNGVR010000000
VERSION     DBNGVR010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2577110"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1363
                     /locus_tag="AC16A0_01345"
     CDS             134..1363
                     /locus_tag="AC16A0_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3104001.1"
                     /translation="MTQALAPSRWFYAPVIVVVAGCLVAMVNFGVRSSFGLFTAPISE
                     ANGWPREIFSFAMAMQNLLWGIATPIAGALADRYGAARVLMLGAVFHAIGTLMMALTS
                     TPVMFNIGGGILVGIGVALSSFGIVMAALGRIVPPEKRSWAFGIATAAGSFGQFIFAP
                     LTGALIAAYGWQNALLVLAASTLVIIVFALPLMVQNRTAGRPQQGEADMTMREAIGTA
                     FGHGSYLLLVAGFFVCGFQLAFITVHLPPYLAEHGISKEFAGLAIGAIGLFNVVGSYA
                     SGVIGGRGEKRIPLSMIYLLRSIAIAAFVLLPITPLTTIIFTASMGLLWLSTVPLTMG
                     LVTVMFGTRYMATLYGFVFLSHQIGSFLGVWLGGRLYDQLGSYDPVWWMGVALGIFAA
                     IVNLPIREQRNPKFAAA"
     gene            1366..1740
                     /locus_tag="AC16A0_01350"
     CDS             1366..1740
                     /locus_tag="AC16A0_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104002.1"
                     /translation="MRAWPGHRLALSLFLGGLAIWIAVMAVVMRHAALPAEASGTMLA
                     VFEPGTTGDQAFASITRAGARPIRETAFGFIWVVADEQPGLAGRLEREGAIGAYRDLP
                     LSPTIAGCFALADAKMANLWAP"
     gene            1870..2139
                     /locus_tag="AC16A0_01355"
     CDS             1870..2139
                     /locus_tag="AC16A0_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HIR3104003.1"
                     /translation="MSDQDFACQLRGWSLTTAEIMYRMPDYRQILQTYIWQDYDLAPK
                     FPKLIKFLDFWSHNLHGPLASVRVAHAGIVGPVQLKHVDQEWRLH"
     gene            complement(2151..2468)
                     /locus_tag="AC16A0_01360"
     CDS             complement(2151..2468)
                     /locus_tag="AC16A0_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104004.1"
                     /translation="MKMRQTRAQSSEPEPARDTAPAKANPGMNGREVADYIADMTLQL
                     SRLARSAGLTSVMVPLEFAYYEAFAAANKVEIPPEERRYLKTLEEACRSFTETPDGSD
                     KPT"
     gene            2631..>3244
                     /locus_tag="AC16A0_01365"
     CDS             2631..>3244
                     /locus_tag="AC16A0_01365"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR3104005.1"
                     /translation="MTVVVVESPAKAKTINKYLGKDFTVLASYGHVRDLPAKDGSVRP
                     DEDFDMSWEVDGKSAKRLADIAAALKSDDRLILATDPDREGEAISWHVLEVLKKKGAL
                     KGKDVKRVVFNAITKQSVLEAMQHPRDVDADLVDAYLARRALDYLVGFSLSPVLWRKL
                     PGSRSAGRVQSVALRLICERESEIETFKAREYWTLAADFLTAKGA"
BASE COUNT          515 a         1062 c         1005 g          662 t
ORIGIN      
        1 tgggcgaaca actgacctgg ccgctgctgc tgggcggcag cctgatcatt gccggggtcg
       61 gcatcgccca gttcggcccg catatgcgca aaacgcgctt ggcagcgctg ccagcatcac
      121 caagcacgac tccatgacgc aagctctcgc tcccagccgc tggttctacg cgccggtcat
      181 tgtcgtcgtc gcgggctgcc ttgtggccat ggtcaacttc ggcgtgcggt caagcttcgg
      241 cctcttcacc gcgccgatct ccgaggccaa tggctggccg cgcgagatct tctctttcgc
      301 catggccatg cagaacctcc tgtggggcat tgcaacgcca atcgccggcg ctctcgccga
      361 ccgctatggc gcggcccgcg tgctgatgct gggtgcggtg ttccatgcca tcggcacgct
      421 gatgatggcg ctgacctcca cgcccgtcat gttcaatatc ggcggcggca tcctggtcgg
      481 catcggcgtt gcgctgtcgt ccttcggaat cgtcatggca gcgctgggcc gcatcgtgcc
      541 ccccgagaag cggtcctggg ccttcggcat cgccacggca gcgggctcct tcggccagtt
      601 catcttcgcg ccgctgaccg gggccctgat cgccgcctat ggctggcaga atgcgcttct
      661 cgtcctggca gcctccacgc ttgtcatcat cgtcttcgcg ctgccgctga tggtgcagaa
      721 ccgaaccgcc ggccggccgc agcaaggcga ggccgacatg accatgcgcg aagcgatcgg
      781 cacggccttc ggccatggtt cctacctgct gctggtggcg ggcttcttcg tctgcggttt
      841 ccagctggcc ttcatcaccg tgcatctgcc gccctatctg gccgagcacg gcatctcgaa
      901 ggagttcgcg ggccttgcca tcggcgccat cggcctgttc aatgtcgtgg gctcctatgc
      961 ctccggcgtc atcggcgggc gcggcgagaa gcgcatcccg ctgagcatga tctaccttct
     1021 gcgctcgatc gctattgcgg cctttgtgct gctgccaatc acaccgctga cgacgatcat
     1081 cttcaccgca tcgatgggat tgctgtggct ttccaccgtg ccgctgacca tgggcctcgt
     1141 cacggtgatg ttcggcaccc gctacatggc gacgctctat ggatttgtct tcctcagcca
     1201 ccagatcggg tccttcctcg gcgtctggct gggcggacgc ctctacgacc agctgggcag
     1261 ctatgacccg gtctggtgga tgggcgtggc gctggggatt ttcgcggcca tcgtcaacct
     1321 tccgatccgt gagcagcgca acccgaaatt cgccgcggcc tagccatgag ggcatggccc
     1381 ggtcaccggc tggccctgtc gctctttctg ggcgggctgg ccatctggat cgccgtgatg
     1441 gccgtggtca tgcgccatgc ggcgctgccc gccgaggcca gcggcacgat gctggcggtc
     1501 ttcgaaccgg gaaccaccgg tgaccaggcc tttgccagca tcacccgcgc cggcgcccgc
     1561 ccgatccgcg aaaccgcctt cggcttcatc tgggtggtgg cggacgagca gcccggtctc
     1621 gccggccggc tggaacggga gggtgccatc ggcgcctatc gggaccttcc gctgagcccc
     1681 accatcgccg gttgcttcgc cctggccgat gccaagatgg ccaatctttg ggcaccttag
     1741 cagcactcat tacgaatgac tgccaacatg ctgatttcaa acattaattc ttcattcatg
     1801 cgcttgaccg catgatcgag ttgaactggt attggagggt tcgtcgctac gactcacgga
     1861 gggaacgaga tgagcgatca agactttgcc tgtcagttgc gtggctggag tctcaccaca
     1921 gccgagatca tgtatcgcat gcccgattac cgccagatcc tgcagaccta tatctggcag
     1981 gactacgatc tggcgccgaa gttccccaag ctcataaagt tcctcgactt ctggtcacac
     2041 aaccttcacg ggccgctggc ctcggtgcgg gtggcacatg ccgggatcgt cggacccgtt
     2101 cagctgaagc acgtcgacca ggagtggcgc ctccactagg cgtctcgatc tcacgtgggc
     2161 ttgtcggagc cgtccggcgt ctcggtgaag ctccggcagg cctcctccag ggtcttgagg
     2221 tagcggcgtt cctcgggcgg aatctccacc ttgttggccg ctgcgaaggc ttcgtagtaa
     2281 gcgaactcca gggggaccat gacgctggtc agcccggccg atcgcgccag acggctgagc
     2341 tgaagggtca tgtcggcgat gtagtcggcc acctcgcggc cgttcatgcc cggattggcc
     2401 ttggccggcg ccgtatctct ggcaggttct ggctcggaag actgggcgcg tgtctgccgc
     2461 atcttcatcg cagtcgtctc caatagtccc gaatgcacgc actatatagg tgtgtgcccg
     2521 cagattaagc aactcctgct tgcatttcag cgtaaatcac gccgatttgg tctgcttgcg
     2581 ggccccaatg gatgcctata tgaggctggc acgttagctc aggaccctga atgaccgtcg
     2641 tcgtcgtcga atctccggcc aaggccaaga ccatcaacaa gtatctgggc aaggacttca
     2701 ccgttctggc ctcctacggt catgtccgcg acctgccggc caaggatggc tccgtccggc
     2761 ctgacgagga tttcgacatg tcctgggagg tcgatggcaa gtcggccaag cggctggccg
     2821 acatcgcggc cgcgctcaaa tccgatgacc gcctgatcct ggcgaccgac cccgaccgcg
     2881 agggcgaagc catctcctgg catgtgctgg aggttctgaa gaagaaggga gcgcttaagg
     2941 gcaaggacgt gaagcgtgtg gtcttcaacg cgatcaccaa acaatctgtc ctagaggcaa
     3001 tgcagcaccc cagggatgtc gatgcggacc tggtggatgc ctatcttgcc cgccgcgcgc
     3061 tggactatct cgtcgggttc tcgctgtcgc ccgtgttgtg gcgcaagctg ccgggatcgc
     3121 ggtcggccgg ccgcgtccag tcggtggcgc tgcgcctgat ctgcgagcgc gaaagcgaga
     3181 tcgagacctt caaggcccgc gaatactgga ccctggcggc ggacttcctg accgccaagg
     3241 gcgc
//
LOCUS       DBNGVR010000065         4553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4738177, whole genome shotgun sequence.
ACCESSION   DBNGVR010000065 DBNGVR010000000
VERSION     DBNGVR010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4738177"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="AC16A0_01370"
     CDS             <1..591
                     /locus_tag="AC16A0_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104006.1"
                     /translation="WNVFCICCIAGFDPFKTHSDEEVAKFTETAKRVFKGAKVVGDIA
                     TMNQSLVAGEIDFHLTGGTYSASPARADGHPEIRGITPKTGPMAGGKGGISWIEITST
                     VNNPELSPLAADFLEYVQSPEMSHTVAFAEGTFNPVSQMGNPDCFKLFTKEELDAIQW
                     DSLAEEMSRSVEYDIVPDYDKLLDIMTAAKREASGG"
     gene            665..1732
                     /locus_tag="AC16A0_01375"
     CDS             665..1732
                     /locus_tag="AC16A0_01375"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006697826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104007.1"
                     /translation="MPDNQPILSLKAVRKMFGPQAAVDSIDLDIAEGEFFTIVGPSGS
                     GKTTMLRMLAGMDTPTSGDILLRGERINDIPANKRPTCMVFQSLALFPHRTVGQNIEF
                     SLKIRGEAPEKRKARALQLMDLLRLPQSYYGKNVLKCSGGERQRVALARALAYDPQIL
                     FFDEPLSAIDYKLRKTLEKELKDIHRETRKTFVYITHSLEEAMVMSDRIGVMRAGRLV
                     QVGTPQQIYSTPNTRFVSEFVGDVNVLPVSRGHDGKLHDATTGLHLKAPPVPQDFVQG
                     HMVIRPEFLRFLESPEQADNAVSGRLYNEYALGSRIQYQVRVGDSVFVVEKLRQQPFT
                     GKLDDEVIIGWDARDAILVTD"
     gene            1735..2577
                     /locus_tag="AC16A0_01380"
     CDS             1735..2577
                     /locus_tag="AC16A0_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104008.1"
                     /translation="MALAQGTRSAIPVMLFLLAGFAAPLMAVIWFSFMPPRSFSFAGT
                     PTLENYVTIFESTSYISFLWSLGLAVVTVVLLAAICYPVAYGLVRVFGKWATLLTLLF
                     TIPLFVSENIRLYGWVLFLIKNGVLLGSLKSLFGLQLESMLFTVPAIVLGMVYVYLPF
                     MLFPMTLGISMVPNETRDAAFDLGATRWQVFKEVELPLSMPGVVIGFLLTFVLAVGAI
                     AEAKVLGGQSVIPITHDIEIAFTYSQNWPLGSALAVLLMLVVGTLVVLALRRFDLDTF
                     LGRR"
     gene            2581..3405
                     /locus_tag="AC16A0_01385"
     CDS             2581..3405
                     /locus_tag="AC16A0_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104009.1"
                     /translation="METRGQRTRHALITVWTLAVIVFLYLPTICIALASLTASRYFQF
                     PISKWGLDWWAKTFNSIEIRQLLSTSITIALCVTVIAVVIAFFGALAFARYDWKGRSV
                     YQKLILLPIFFPQSVLGLALLLWFSTLGLPLSWKTAVFAHLVWIVPVVTLVISIQVYS
                     FDPALEEAAFDLGATRWQVLREVTLPVLFPGLFSGALFAFLLSWGNFPLSLFTTGADT
                     TVPEYLYAKMVAGYTPGVPVLGTVSTIGATILLIGGYGLILAMRRAKTQDETGRTS"
     gene            3402..4172
                     /gene="fabG"
                     /locus_tag="AC16A0_01390"
     CDS             3402..4172
                     /gene="fabG"
                     /locus_tag="AC16A0_01390"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3104010.1"
                     /translation="MTQSLKGRSAVVTGGSKGIGKGIARVLANAGAKVAIVARHADQA
                     EAAAKEIGHGAFGLAGDVTSMASMDKVMKTVADKNGGLDVLCANAGIFPPAKLEEMTS
                     EQWDEVVDTNLKGTFHSVKAAIPYLKTSDQGRIVLTSSITGPITGFPGWTHYGATKAG
                     QLGFMRTACIELARYGITVNAVLPGNILTEGLIGMGEEYQKTMAASIPLKKLGTVEDI
                     GYASLYFASKEAGYVTGQTIIVDGGQILPESLEALNQA"
     gene            complement(4177..>4553)
                     /locus_tag="AC16A0_01395"
     CDS             complement(4177..>4553)
                     /locus_tag="AC16A0_01395"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR3104011.1"
                     /translation="ERFGLIHCDLRLANLLIDGKAVKVIDFDDCGFGWYMYDAATPVS
                     FYEHEPQVPSLIESWKTGYRRVIDLSRADEAEIPTFVMLRRLLLVAWIGSHAETDLAK
                     SMGPPYTEGTMGLCEDYLRKFG"
BASE COUNT          807 a         1475 c         1365 g          906 t
ORIGIN      
        1 tggaacgtct tctgcatctg ctgcatcgcc ggattcgacc ccttcaagac acactcggac
       61 gaggaagtgg cgaagtttac cgagactgca aagcgcgtgt tcaagggcgc caaggtggtg
      121 ggtgacattg cgaccatgaa ccagtcgctg gtcgctggcg agattgactt ccatctgacg
      181 ggcggcacct actcggcctc gcccgcgcgc gccgacggtc atccggagat ccgcggcatc
      241 acgcccaaga caggaccgat ggccggcggc aagggcggca tctcatggat cgagatcacc
      301 tcaacggtga acaatccgga actctcgccg ctggcggcgg acttccttga gtatgtccag
      361 tcgcccgaga tgtcgcatac ggtggccttt gccgaaggca ctttcaatcc cgtctcgcag
      421 atgggcaatc cggactgttt caagctcttc accaaggaag agctcgacgc gatccagtgg
      481 gacagtctcg ccgaggagat gtcgcgctcg gtcgaatacg acatcgtgcc ggactacgac
      541 aagctgctcg acatcatgac ggcggcgaag cgcgaagcat ccggcggctg atcgcgtcac
      601 catcgacact gccccctgcg atccgctcgc agggggtcgt ttttgcggag agtgccagga
      661 cccgatgccc gataaccaac cgatcctctc gctcaaggcg gtgcgcaaga tgttcggccc
      721 gcaggcggcg gtcgattcga tcgacctcga cattgccgag ggcgagttct tcaccatcgt
      781 tgggccgtcc ggctcgggca agaccaccat gctgcgcatg ctggcgggca tggacacccc
      841 gaccagcggc gacatcctgc tgcgcggcga gcgcatcaac gacattccgg ccaacaagcg
      901 gcccacctgc atggtgttcc agtcgctggc cctcttcccg caccggacgg tggggcagaa
      961 catcgagttt tcgctgaaga tccgcggcga ggcgccggag aagcgcaagg cacgcgcgct
     1021 gcaactgatg gatctcctgc gcctgccgca gtcctactac ggcaagaacg tgctcaaatg
     1081 ttccggcggc gagcgccagc gcgtggcgct ggcccgggcg ctggcctatg atccgcagat
     1141 cctgttcttc gacgagccat tgtcggccat cgactacaaa ctgcgcaaga ctcttgagaa
     1201 ggaactcaag gacatccatc gcgagactcg caagaccttc gtctacatca cccattccct
     1261 cgaggaggcc atggtgatga gtgatcgcat cggcgtcatg cgcgcgggca ggctggttca
     1321 ggtcggaacg ccgcaacaga tctactcgac gcccaatacg cgtttcgtgt cggagttcgt
     1381 cggcgacgtg aatgtcctgc ccgtgagtcg tggtcatgac ggcaagctgc atgacgccac
     1441 caccggactt cacctcaagg caccgccagt cccgcaggat tttgtccagg gtcacatggt
     1501 aatccgcccg gagttcctgc gctttctcga gtcgccggaa caagccgaca acgccgtgtc
     1561 cgggcgcctc tacaacgaat atgccctcgg gtcccgaatc cagtaccagg tccgcgtcgg
     1621 cgacagcgtc ttcgtggtcg agaagctccg ccagcagccc ttcaccggaa agctcgatga
     1681 cgaggtgatc atcggctggg atgccaggga cgcgatactg gtgacggact gatcatggcg
     1741 ctcgcgcaag gcacaaggtc cgccatcccg gtcatgctct tcctgctggc cggcttcgcc
     1801 gcgcccctga tggcggtcat ctggttcagc ttcatgccgc cgcgcagctt ctccttcgcc
     1861 ggcacgccga cgctcgagaa ctatgtgacg atcttcgaaa gcacgagcta catttccttc
     1921 ctgtggtccc ttgggcttgc ggtcgtcacc gtcgtcctgc tggccgccat ctgctaccct
     1981 gttgcctacg ggctcgtacg cgtctttggc aaatgggcaa cgctgctgac tctgcttttc
     2041 accattccgc tcttcgtctc cgagaatatc cgcctctatg gctgggtact cttcctgatc
     2101 aagaacgggg tgctgctggg ttcgctcaag tcgctctttg gccttcaact cgaaagcatg
     2161 ctcttcaccg taccggccat cgtgctgggc atggtctatg tttacctgcc attcatgctc
     2221 tttcccatga cgctcgggat cagcatggtt ccaaatgaaa cccgcgacgc ggcctttgac
     2281 cttggcgcga cgcggtggca ggtcttcaag gaggtggaac tgccgctgtc catgccgggc
     2341 gtggtcatcg gctttctgct gaccttcgtc ctcgccgtcg gcgccattgc cgaggccaag
     2401 gtgctgggcg ggcagagcgt catcccgatc acccacgaca tcgagatcgc cttcacctat
     2461 tcgcagaact ggccgctcgg atcggcactg gcggttctgc tgatgctggt ggtcggcacg
     2521 ctggtcgtcc tggcgttgcg gcgcttcgac ctcgacacgt tcctcgggcg gagataggcg
     2581 atggagacgc gaggccagcg cacccgccac gccctcatca ccgtctggac gcttgcggtg
     2641 atcgtgttcc tctatcttcc gaccatctgc atcgcgcttg cctcgctcac cgccagccgc
     2701 tacttccagt ttcccatcag caagtggggt ctcgactggt gggccaagac gttcaactcg
     2761 atcgagatcc gccagctgct ctcgacatcc atcaccattg ccctgtgcgt caccgtcatc
     2821 gcggttgtca tcgccttctt cggcgccctc gcttttgcgc gctacgactg gaagggacgt
     2881 tcggtctatc agaagctcat cctgctgccg atcttctttc cgcagtcggt gctggggctt
     2941 gccctgctgc tgtggttcag cactctgggg ttgccgctct cctggaagac cgccgtcttc
     3001 gcgcatctcg tgtggatcgt gccggtggtg acgctggtga tctcgataca ggtctacagc
     3061 ttcgatcccg cgctggaaga ggccgccttc gatctcggcg ccacgcgctg gcaggtgctg
     3121 cgcgaggtaa cgcttccggt gctctttccc ggacttttct cgggcgcgct cttcgccttc
     3181 ctgctgtcct ggggcaattt tccgctgtcg ctcttcacca ccggcgccga caccacggtg
     3241 ccggagtatc tctatgccaa gatggtggct ggctatacgc caggcgttcc ggtgctcggc
     3301 accgtgtcga ccatcggggc gacgattctg ctcattggag gctatggcct gatcctggcc
     3361 atgcgccgcg ccaagacaca agacgagact gggaggacat catgactcaa tcgctgaagg
     3421 gccgctccgc cgtggtgact ggcggatcca agggaatcgg caagggcatt gcccgcgttc
     3481 tggcaaatgc cggtgcaaaa gttgcgatcg tggcgcgcca tgccgaccag gcggaagcgg
     3541 cggcgaagga gatcggccat ggcgccttcg gactcgccgg cgacgtcacc tccatggcca
     3601 gcatggataa ggtcatgaag accgtcgccg acaagaacgg cggcctcgac gtgctctgcg
     3661 ccaatgccgg catcttcccg ccggccaagc tggaggagat gacctccgag caatgggacg
     3721 aggtcgtaga cacgaacttg aagggcacct tccattcggt gaaggccgcc attccctatc
     3781 tcaagacatc cgatcagggc cgcatcgtcc tgacctcgtc catcaccgga ccaatcaccg
     3841 gctttcccgg ctggacgcat tacggtgcca ccaaggctgg ccagctcggc ttcatgcgta
     3901 ccgcctgcat cgagctggcg cgctacggca tcaccgtgaa tgcggttctt cccggcaaca
     3961 tcctcaccga aggccttatc ggcatgggcg aggaatatca gaagaccatg gccgcctcga
     4021 tcccgctcaa gaagctcggc acggtggagg acatcggcta tgcgtccctc tactttgcct
     4081 cgaaggaggc aggctatgtc acggggcaga ccatcatcgt cgacggcggc cagatcctgc
     4141 cggaaagcct cgaggccctg aaccaggcct gatgtatcag ccgaacttgc ggagatagtc
     4201 ctcgcagagt cccatggtgc cttccgtgta gggagggccc atggatttgg cgaggtcggt
     4261 ttcggcatgc gagccgatcc aggcgaccag cagcagacgg cgcagcatca cgaaggtggg
     4321 aatctccgcc tcatccgcgc ggctgagatc gatcacccgg cgatagcccg tcttccacga
     4381 ctcgatgagg gacggcacct gcggctcgtg ttcgtagaag gagaccggcg tcgccgcatc
     4441 atacatgtac cagccgaagc cgcagtcgtc gaagtcgatg accttcaccg ccttgccgtc
     4501 gatcagcagg ttggccaggc gcaggtcgca gtggatcagt ccgaagcgct ccg
//
LOCUS       DBNGVR010000066         5145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1398762, whole genome shotgun sequence.
ACCESSION   DBNGVR010000066 DBNGVR010000000
VERSION     DBNGVR010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1398762"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16A0_01400"
     CDS             complement(<1..230)
                     /locus_tag="AC16A0_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR3104012.1"
                     /translation="MPEFEDWIGRRADTPDAATPRLLAQFRATLAPHLFEPGDPDVAP
                     PGFHWCLAPALPAAAELGEDGSAAHAGLVPPVP"
     gene            366..1568
                     /locus_tag="AC16A0_01405"
     CDS             366..1568
                     /locus_tag="AC16A0_01405"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3104013.1"
                     /translation="MDTALKSPAAPPGNDWDRRGLPAWTYHSEALLQLEIDEIFLNHW
                     QLAGHVNDVPKKGDYCTLDIANERALIVRGDDGEVRAFHNLCRHRGSRVVGDKQGHCK
                     NAIICPFHGWVYNFDGTLRGAARPNSFAGLDKTKFGLKPLDTEVWMGFVFIRFNKGPQ
                     PSVAELMARFVPELSHYKVEDMVPAGSFYEFTTGVNWKSVRDVDNEGYHVAMAHPALQ
                     DLYGATYFDEPFENGISRSTGAYSTHGGRRWSVRNYLKHAPLQMSLPEDLRRRWTYYG
                     LFPNCVIAMTPEVVQFYQEFPLGVSKTLQRGAIYKRPVETREEKLARYLARRIDRDTG
                     REDVQLTIWSNESMKSKAFDDFHLSDLELGVKSHHDHVRNALPVVRLQEEPRDRPIAE
                     VNRQLTEA"
     gene            1650..2759
                     /locus_tag="AC16A0_01410"
     CDS             1650..2759
                     /locus_tag="AC16A0_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3104014.1"
                     /translation="MTRINRRAALAALGGATALSMPYIRRAHADAGTLNVYNWADYIG
                     ETTIEDFQKATGIAVTYDTYSSAEEMQAKMLAGSTGYDVVDMSGMEMPRFIKAGIYQK
                     LDRAKLPSWSNLDPEVLRILAGWDPGNEYAIPYMWGSVGITYNVDMIKERLPDADLES
                     LDTIFKPENAAKLADCGISILDSPSDVILMMLKYLGKDGDTKNVADYQAVVEAFKPVR
                     EHIRTFDNTNYLNAIPNKELCVINNWSGDYSTAKARAEEAGVEINLAYFVPKTGAPAW
                     VDCLCIPTDAPNVENAYKFLEYMLQPEVIAKCTNFTNYANGNIASKPFIDPAVLANPA
                     VYPDEATLKRLWAPAPLDDDQNAGMTRAWSDIKSG"
     gene            2809..3930
                     /locus_tag="AC16A0_01415"
     CDS             2809..3930
                     /locus_tag="AC16A0_01415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104015.1"
                     /translation="MAATDTGVAAAPFVRIEGVTKTFGTFKAVDNVSLDIAKGELFSL
                     LGGSGCGKTTLLRMLAGFEQPTSGRIFIDGQDMTSLPPYERPVNMMFQSYALFPHMSV
                     EQNVGYGLKHEDLTTAQRSARVKEMLDLVKLSELAGRKPHQLSGGQRQRVALARALSK
                     QPKLLLLDEPLGALDKKLREHTQFELMNIQDRTGITFIVVTHDQEEAMTLSSRIAVMD
                     RGVIRQVGTPNEIYEFPSNRFVADFIGSINQFEGEVIRTGGGMTDVSCPVLSGTVKAA
                     TDATPERGSKVTVAVRPEKITITRKAPSGGVNVVRGTVRDLGYFGKDSLYRVKLESGH
                     LVLVNSVNARRIDEGGRVAQWEDEVWLSFEPSSAILLTD"
     gene            3935..4843
                     /locus_tag="AC16A0_01420"
     CDS             3935..4843
                     /locus_tag="AC16A0_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104016.1"
                     /translation="MRDWFNRTGWRDLIIATPYAWLIVFFLIPFLIVVVMSLAIMKSA
                     SPPFGFHEAFPWIDLGNFARLFQDTLYIRAFVSSLLNALIATLLCLLIGYPMALGITR
                     VSKSWRNILLMLVILPFWTSFLLRVYAWIGMMGSNSWFNKLLTSIYNFAVPGEWALKS
                     IPMMNTSFAVVLVMVYSYLPFMILPLYANLEKLDTTLNEAAMDLGSRPWQVFRDITLP
                     LSIPGIIAGGLLVFIPATGELIIPSLVGSSANPMIGRVIADEFNSARDWPMASAVAVA
                     LLFLMVLPMVFYNHYEAKAQNAGGKP"
     gene            4840..>5145
                     /locus_tag="AC16A0_01425"
     CDS             4840..>5145
                     /locus_tag="AC16A0_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HIR3104017.1"
                     /translation="MTRRPLFLISCLCFGFAFFYIPILSMIFFSFNKSRLATVWGGFS
                     LQWYGKLFDNDQIMNAALLSLQIALVSATFATILGTLCGLALARFTQFRGRTLFSGLV
                     "
BASE COUNT         1015 a         1656 c         1490 g          984 t
ORIGIN      
        1 ggaacgggcg gaaccagccc ggcatgggcc gcactgccat cctcgcccag ttcggcggcg
       61 gccggcaagg caggcgcaag acaccagtga aaccccggcg gcgccacatc gggatcgccg
      121 ggttcgaaca gatgcggtgc gagcgtggcg cggaattggg ccagcaggcg cggggtcgcg
      181 gcatcgggcg tgtctgcccg ccggccgatc caatcctcaa attctggcat ctgcagggtc
      241 tttccttgct tcaacacata tcatcaactc ttttaaaatc aataagataa cagattcgac
      301 tggattttga atgatagttc agtcaatata catgtccaaa cccgtcttga attcggagat
      361 gatccatgga caccgcgctc aaatcgcccg ccgccccgcc tggcaatgac tgggaccgcc
      421 gtggcctgcc ggcctggacc tatcacagcg aagccctgct tcagctggag atcgacgaga
      481 tcttcctcaa tcactggcag ctggccggcc atgtgaacga cgttccgaag aagggcgact
      541 actgcactct cgacatcgcc aacgagcgtg ccctgatcgt gcggggcgac gatggagagg
      601 tgcgcgcctt ccacaatctc tgccgccatc ggggctcccg cgtggtcggc gacaagcagg
      661 gccactgcaa gaatgcgatc atctgcccct tccatggctg ggtctataac ttcgacggca
      721 cgctccgcgg tgccgcccgt cccaatagct tcgccgggct cgacaagacg aagttcggcc
      781 tgaagcctct ggacaccgaa gtctggatgg gcttcgtctt catccgcttc aacaagggtc
      841 cgcagccgtc ggtcgccgaa ctgatggcgc gtttcgttcc ggagctttcg cactacaagg
      901 tggaagacat ggtgccggcc ggcagtttct acgagttcac cactggtgtg aactggaagt
      961 cggtgcgcga cgtcgacaat gagggctacc atgtggcaat ggcccatcct gcactgcagg
     1021 atctttatgg ggcaacctat tttgacgagc ccttcgaaaa tggcatcagc cgctcgaccg
     1081 gagcctatag cactcatggc ggtcggcgct ggagcgtccg caactatctg aaacacgccc
     1141 ctctccagat gtcccttccc gaagacctgc gccggcgctg gacctactac ggcctcttcc
     1201 ccaactgcgt gatcgcgatg acaccggaag tcgtgcagtt ctatcaggag tttccgttgg
     1261 gcgtatccaa gaccctgcaa cgcggtgcca tctacaagcg gccggtcgag acgcgggagg
     1321 agaagctcgc gcgctatctc gccagacgca tcgaccgcga caccgggcgc gaggacgttc
     1381 agcttaccat ctggtcgaat gagtcgatga agtccaaggc cttcgacgat ttccacctgt
     1441 ccgatctgga actgggcgtg aagagccacc acgaccacgt tcgcaacgcc ctgccggtcg
     1501 tgaggttgca ggaggaacct cgggatcggc ccatcgccga ggtcaaccgt cagctcacgg
     1561 aagcttaaat tgacatcgga gcgattccgg tttgaactgc cgttcaacga caacgatggg
     1621 cacggcaaac agtgtccaag gaggaaacca tgacgagaat caaccgacgt gcggcactgg
     1681 ccgcactggg cggcgcgacc gcgctgtcca tgccttacat acgtcgcgcc catgccgatg
     1741 ccggcacact caacgtctat aactgggccg actatatcgg cgaaaccacc atcgaggact
     1801 ttcagaaggc caccggcatc gccgtgacct atgacaccta tagctcggcc gaggaaatgc
     1861 aggccaagat gctggccggc agcaccggct atgatgtggt cgacatgtcg ggcatggaaa
     1921 tgccgcgctt catcaaggcc ggcatctacc agaaacttga ccgcgccaag cttccaagct
     1981 ggagcaatct cgatcccgag gtcttgcgca ttctggccgg atgggatccc ggcaatgaat
     2041 atgcgatccc ctacatgtgg gggtcggtgg gcatcaccta caacgtcgac atgatcaagg
     2101 agcggctgcc ggatgccgac ctcgagtccc tcgacaccat cttcaaaccg gagaatgcgg
     2161 ccaagctggc cgattgcggc atttcgatcc tcgactcgcc gtccgacgtc attctcatga
     2221 tgctgaagta cctgggcaag gatggcgaca caaagaacgt ggccgactac caggccgtgg
     2281 tcgaggcctt caagccggtg cgcgagcaca tccgcacctt cgacaacacc aactacctga
     2341 acgcaatccc caacaaggaa ctctgcgtca tcaacaactg gtcgggcgac tactccacgg
     2401 ccaaggcccg tgccgaggaa gccggtgtcg aaatcaatct cgcctacttc gtgccgaaga
     2461 cgggcgctcc cgcctgggtc gactgcctct gcatcccgac cgatgcgccc aacgtggaaa
     2521 acgcctacaa gttcctcgaa tacatgctgc agccggaggt gatcgcgaag tgcacgaact
     2581 tcacgaacta cgccaacggc aatatcgctt ccaagccatt catcgatccg gcggtcctgg
     2641 ccaacccggc cgtctatccc gatgaggcga cgttgaagcg cctctgggct cccgcgcctc
     2701 tggacgacga ccagaatgcc ggaatgaccc gcgcctggag cgacatcaag agcggttgaa
     2761 gacgaaagcc cggccctcag aggccgggct tcctactctg gggggagcat ggccgcaaca
     2821 gataccggtg tagccgcggc accattcgtc aggatcgagg gggttaccaa gacgttcggc
     2881 accttcaagg ccgtcgacaa cgtctcgctc gacattgcaa aaggggagct tttctcgctg
     2941 ctcggcggat cgggctgcgg caagaccacg ctgctgcgca tgctcgccgg attcgagcag
     3001 ccgacgtcgg gccggatctt catcgacggc caggacatga cgtcgctgcc gccctatgag
     3061 cggccggtca acatgatgtt ccagtcctat gcgctgtttc cgcacatgag cgtcgaacag
     3121 aacgtcggct acggcctgaa gcatgaggac ctgacgacgg cccagcgcag cgcgcgcgtc
     3181 aaggagatgc ttgatctggt gaaactctcg gagctggccg gacgcaagcc gcaccagctc
     3241 tcgggcggtc agcgccagag ggtggccctg gcccgtgccc tgtccaagca gcccaagctg
     3301 ctgcttctgg acgaaccgct gggcgccctc gacaagaagc tgcgcgaaca cacacagttc
     3361 gagctgatga acattcagga ccgcacaggc atcaccttca tcgtcgtcac gcacgatcag
     3421 gaagaagcga tgacgctgtc cagccgcatt gccgtcatgg accgcggcgt gatccgtcag
     3481 gttggcacgc ccaacgagat ctacgaattc ccgtccaatc gcttcgtcgc cgacttcatc
     3541 ggttcgatca accagttcga gggggaagtc atccgcacgg gtggcggcat gacggatgtg
     3601 tcctgtccgg ttctgtccgg cacggtgaag gcggcaaccg acgccactcc cgaacggggc
     3661 agcaaggtca cggtcgcggt ccggccggaa aagatcacca tcacgcgcaa ggcgcccagc
     3721 ggcggcgtga acgtggtcag gggaacggtg cgcgacctcg ggtatttcgg aaaggactcg
     3781 ctctaccgcg tcaagctgga gtccgggcat cttgtcctcg tcaacagcgt gaacgcccgc
     3841 cgcatcgatg agggcggccg cgtggcgcaa tgggaagacg aggtctggct cagcttcgaa
     3901 ccatcctccg ccatcctgct gacggattga gaacatgcgc gactggttca atcgcacggg
     3961 ctggcgcgac ctcatcatcg cgacgccata cgcttggctc atcgtcttct tcctgattcc
     4021 cttcctgatc gttgtcgtca tgagtctggc gatcatgaag tccgcttccc cgcccttcgg
     4081 ctttcatgag gcgtttccct ggatcgacct tggcaacttc gcgcgactct tccaggatac
     4141 gctctatatc agggccttcg tgagctcgct gctcaatgca ctcattgcca cgttgctctg
     4201 cctgctgatc ggctatccga tggccctggg catcacgcgg gtctcgaaga gctggcgcaa
     4261 catcctgctc atgctggtga tcctgccctt ctggacctcc ttcctgctgc gcgtctatgc
     4321 ctggatcggc atgatggggt ctaacagctg gttcaacaag ttgctgacca gcatctacaa
     4381 cttcgcggtt cccggcgaat gggcgctcaa gtcgatcccg atgatgaaca ccagtttcgc
     4441 ggtggtgctc gtcatggtct actcctacct gcccttcatg atcctgccgc tctacgccaa
     4501 cctcgagaag ctcgacacga ccctgaacga ggccgccatg gatctgggat cgcggccctg
     4561 gcaggtgttc agggacataa cccttccgct gtcgataccg ggcatcatcg cgggcggcct
     4621 cctcgtcttc attcccgcga ccggcgaact gatcatcccc agcctggtcg gcagttccgc
     4681 caatccgatg atcggccgcg tgatcgccga cgaattcaac tcggcacgcg actggcccat
     4741 ggcgtctgcc gtcgcggtgg cgctgctctt cctgatggtg ctgcccatgg tgttctacaa
     4801 ccactatgag gctaaggcgc agaatgccgg gggcaagcca tgacgaggcg gcccctcttc
     4861 ctcatctcct gcctctgctt cggattcgca ttcttctaca ttccgatcct gtcgatgatc
     4921 ttcttctcct tcaacaagtc gcggctggcg accgtctggg gcggtttctc gctgcagtgg
     4981 tacggcaagc tcttcgacaa tgaccagatc atgaatgcag cgctgctgtc gctgcagatc
     5041 gcgctggtca gcgccacctt cgcaaccatc ctcggcacgc tctgcggcct ggcccttgcg
     5101 cggttcacgc agttccgcgg ccgcacgctg ttctccggcc tggtc
//
LOCUS       DBNGVR010000067         3262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1399257, whole genome shotgun sequence.
ACCESSION   DBNGVR010000067 DBNGVR010000000
VERSION     DBNGVR010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1399257"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC16A0_01430"
     CDS             complement(<1..341)
                     /locus_tag="AC16A0_01430"
                     /inference="COORDINATES: protein motif:HMM:NF018446.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR3104018.1"
                     /translation="MSDVEHPTQTEGTFTRESRGLEFERVAFFSDAIYAIAMTLLVVD
                     IKVPELGQSNKDPWMVLDTLRENTPEIIGFFLCFWLIGNTWISHHRFMASLHTIGHTL
                     MTLNMFYLAFVA"
     gene            complement(338..736)
                     /locus_tag="AC16A0_01435"
     CDS             complement(338..736)
                     /locus_tag="AC16A0_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3104019.1"
                     /translation="MADCKLIRGSEAFHGKQGLDYFSGISAESAGSQALCMHLVTIPP
                     GGSARPHYHAHHESAIFVLEGYSEMRHGPALEHVMEIRAGDFCYIPAGVPHQPYNPTG
                     KPVRAVIARTDPREQESVVLLPPDFGSANP"
     gene            783..1406
                     /locus_tag="AC16A0_01440"
     CDS             783..1406
                     /locus_tag="AC16A0_01440"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB
                     family protein"
                     /protein_id="HIR3104020.1"
                     /translation="MIQGLKHFPQLLDRSQQEELAETLRQAVAAAPLFIPVMPRTGRP
                     FSVRMTNLGQLGWVSDRSGYRYQPRHPDTGQPWPPIPEAVLDVWRRVAAYPAPPEACL
                     VNVYREGARMGLHQDADEEDFAAPVVSISLGDTAVFRIGGQLRGGPTRTLKLSSGDVL
                     VMGGAARLSYHGIDRLVPGSSTLLRDGGRINLTLRRVTRPAPSPSSR"
     gene            complement(1387..1692)
                     /locus_tag="AC16A0_01445"
     CDS             complement(1387..1692)
                     /locus_tag="AC16A0_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019057.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HIR3104021.1"
                     /translation="MPDYKLDVYKDKRGDFRWRRIAENGEIVGASSEGYSSRKHCLAN
                     AERDTATDNWEFYRDKRGGYRWRCFSPANKKQVGKSTEAFSSKKNAENNARINGWKG"
     gene            complement(1849..2406)
                     /gene="grpE"
                     /locus_tag="AC16A0_01450"
     CDS             complement(1849..2406)
                     /gene="grpE"
                     /locus_tag="AC16A0_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3104022.1"
                     /translation="MADETKTPDPQDSEAETPFTDADVDARDLEIAMLKDEVAALKDR
                     LLRTAADMENLRKRAEREKAEATLYAATNFARDLLSVSDNMQRALAAMSQEAKDGADA
                     VTRNLMTGVEMTEKELLNVFQRYGIRKVDTVGHKFDPNLHQALFEVPTKDHPPGTVVQ
                     EMQSGFAIGDRCLRPAMVGVAKAQG"
     gene            complement(2471..>3262)
                     /locus_tag="AC16A0_01455"
     CDS             complement(2471..>3262)
                     /locus_tag="AC16A0_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013768.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HIR3104023.1"
                     /translation="SMSAEERARIDAQIAASNRPRRIEDVLGEATTLLSGLSHCAGVI
                     VTPKMNARLKHIEFVNLAPGRALVILVGEDGGVENRVIDVPRGLPPSTFTEASNYLSS
                     RFQGKTITEVQRVVREEMDGLRRELDEVSARIVEAGVAVWAGDSDPQGKTLIVKGQAN
                     LLENATAVADLERIRRLFDDIENKSELVQLLGLAEAGEGVRIFIGAENRLFSLSGSSL
                     IAAPYKNSEEKIVGVLGIIGPTRMNYARIIPMVDYTAKVLGRLLT"
BASE COUNT          554 a         1025 c         1035 g          648 t
ORIGIN      
        1 gccacgaagg cgagatagaa catgttcagc gtcatcagcg tatggccgat ggtatggagg
       61 ctggccatga agcggtgatg gctgatccag gtgttgccaa tcagccagaa acaaagaaaa
      121 aaccctatga tttcaggtgt gttctccctc agcgtgtcga gcaccatcca ggggtccttg
      181 ttgctctggc caagttccgg gaccttgatg tcgaccacca gcagggtcat tgcgatggcg
      241 tagatggcgt cgctgaagaa ggcgacacgt tcgaattcca gcccccggct ttcccgcgtg
      301 aaggttccct ctgtctgggt cggatgctcg acgtcgctca tggatttgca ctgccgaagt
      361 cagggggcag aagcaccacg ctttcctgct cgcgcgggtc ggtccgggca atgacggcgc
      421 ggacgggctt gccggtcgga ttgtagggct ggtgcggaac gccggccggg atgtagcaga
      481 aatcgccggc gcggatttcc atcacatgtt ccagcgcggg gccatggcgc atctcggaat
      541 agccctccag cacgaagatc gcagactcat gatgtgcgtg gtagtgcggc cgggccgatc
      601 cgccaggcgg gatcgtcacc agatgcatgc agagcgcttg ggagcccgcc gactcggctg
      661 aaatcccgct gaagtagtcc aatccctgct tgccatgaaa ggcttcgctg ccccggatca
      721 gcttgcagtc cgccatgcat cttctccttg cgcaggatca tcgcgggcga gagactatcc
      781 gaatgatcca agggttaaag cactttccgc agctactgga ccgctctcaa caggaggaac
      841 tggcggagac cctgcgccag gccgtggccg ccgcaccatt gttcatccct gtcatgccgc
      901 gcaccgggag gcccttcagt gtccgcatga ccaatctcgg ccagctgggg tgggtttcgg
      961 accggtctgg ctatcgctat cagccgcgcc acccggacac ggggcaaccc tggccgccga
     1021 taccggaggc cgtcctggac gtttggcgcc gtgtcgcggc gtaccccgcg ccgccggaag
     1081 cctgcctcgt gaatgtctac cgggaaggcg ccaggatggg attgcatcag gatgccgacg
     1141 aggaggattt cgcagcgccg gtcgtctcga tttcgctggg cgacacagcc gtgttccgca
     1201 tcggcggaca gctgcgcggc ggcccgaccc gaacgctcaa gctctcgtcc ggcgacgtgc
     1261 tggtgatggg cggtgccgcc cgactcagct accacgggat cgaccggctg gtgcccggtt
     1321 catcgaccct gctgagggat ggcggccgga tcaatctcac ccttcgccgg gtgacccgcc
     1381 cggcgcctag cccttccagc cgttgatgcg ggcgttgttc tccgcgttct tcttggagga
     1441 gaaggcttcc gtcgatttgc ccacctgctt cttgttggcc ggggagaagc agcgccagcg
     1501 gtaaccgcca cgcttgtcgc gatagaattc ccaattgtcg gtggccgtgt cccgttcagc
     1561 attggccagg caatgcttgc gcgaggaata gccttccgaa gaggcgccca cgatttcacc
     1621 gttctcggcg atgcggcgcc agcggaaatc gccgcgcttg tccttgtaga cgtcaagttt
     1681 gtagtcgggc atgtgacctc ctgtaagctg atgtcgttac ttatgaacaa catgcaacta
     1741 ttttgtagag tcacttccac catgtccaga cgctagagga cttctatcct ctgctatctg
     1801 tcggtacctg tcgctgaaag catcccgcct gagccttggc cctgccgctc agccctgcgc
     1861 cttggccaca cccaccatgg cgggtctcag gcagcggtcg ccgatggcga aacccgactg
     1921 catttcctgg accaccgtcc cgggcggatg gtccttggtc ggcacctcga agagggcctg
     1981 atgcaggttg ggatcgaact tgtggccgac ggtgtcgacc ttgcggatgc cgtagcgctg
     2041 gaagacgttg agcagctcct tctcggtcat ttccacgccg gtcatcaggt tgcgggtcac
     2101 cgcatcggcg ccgtccttgg cctcctgcga catggcggcg agcgcgcgtt gcatgttgtc
     2161 ggacacgctc agcagatcgc gggcgaaatt ggtggcggca tagagcgtag cctcggcctt
     2221 ttcgcgctcg gcccgcttgc gcagattttc catgtcggcg gcggtgcgca gcagccggtc
     2281 cttgagggcg gcaacctcgt ccttgagcat ggcgatctcg aggtcgcggg cgtcgacatc
     2341 ggcgtcggtg aagggggttt ccgcctcgga atcctgcggg tcaggggtct tcgtctcgtc
     2401 agccatgctc acgctctctg gtcagtcact cgggaatatc cgccgcatat cggccgtcag
     2461 gccagaaaaa tcaagtgagc aggcggccca ggaccttggc cgtgtaatcc accatgggaa
     2521 tgatccgggc atagttcatc cgggtcggtc cgatgattcc cagcacgccc acgatcttct
     2581 cctcgctgtt cttgtagggt gcagcgatga gggaagaacc ggacagtgaa aacaggcggt
     2641 tctccgcgcc gataaagatg cgcaccccct cgccagcctc ggcgagcccc aggagctgca
     2701 ccagctcgct cttgttctcg atatcgtcga agaggcgccg gatgcgttcc aggtcggcca
     2761 ccgcggtggc gttttccagc agattggcct gacccttgac gatcagggtc ttgccctgcg
     2821 gatcgctgtc acctgcccag acggcaacac cggcttccac gatgcgggcc gagacctcgt
     2881 caagctcgcg gcgcaagccg tccatctcct cgcgcaccac gcgctgcacc tcggtgatgg
     2941 ttttgccctg gaagcgcgac gacagataat tcgaggcttc cgtgaaggta gagggcggca
     3001 ggccgcgcgg cacatcgatg acgcggtttt ccaccccgcc gtcctcgcct accaggatca
     3061 ccagggcgcg gcccggcgcg agattgacga actcgatgtg cttcagccgt gcattcatct
     3121 tgggcgtgac gatcacgccg gcgcagtgtg aaagaccgga cagcagcgtc gtggcctcgc
     3181 ccagcacgtc ttcgatgcgc cgtggccggt tggaggcggc gatctgggcg tcgatgcggg
     3241 cgcgctcctc ggcgctcatc ga
//
LOCUS       DBNGVR010000068         5719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1595102, whole genome shotgun sequence.
ACCESSION   DBNGVR010000068 DBNGVR010000000
VERSION     DBNGVR010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1595102"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="AC16A0_01460"
     CDS             <1..572
                     /locus_tag="AC16A0_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104024.1"
                     /translation="SSATRASANATNMGSIRQSGQQRSMGIEEKAMMKRFGVAIASAV
                     AAAALFSLPGLAQDAERAKMTTEMDLHVTDLADGMLSPYLDKDEQTLMKSLAYQHAVA
                     LNCKGLSVDQAKSQAQLERVFPQAEMDKMTEEQQLAMTTSVMMGFSAVMGGQFAIAAV
                     DYDAYCKAAEADFASKEGNDTHLILVKAP"
     gene            609..1172
                     /locus_tag="AC16A0_01465"
     CDS             609..1172
                     /locus_tag="AC16A0_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3104025.1"
                     /translation="MRLLRRHLLIAPYLFFTRAAHADDGAWAELRGSEIVLFRHANAP
                     GTGDPPGFVVGDCSTQRNLDERGRVQARAIGEAFRNRGIRVGRVLSSQWCRARDTAEL
                     AFPGQVNLEPAFNSFFGNRESEDRQTAEAQRVLRDWSGPGVLVVVTHQVNISALTGIA
                     PKSGEGIVLRLADGQLTVLSRLPAPDA"
     gene            1211..1840
                     /locus_tag="AC16A0_01470"
     CDS             1211..1840
                     /locus_tag="AC16A0_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3104026.1"
                     /translation="MTDQSVTKTRQRRPDARPAEILGAALELFAEKGFSGTRLEDVAA
                     RAGLSKAAIYLYFDGKMDLLRALVQEMAGANLAVAGSIAQGHVGEVAPLLRQILVFMS
                     GQLRHTRFPELMKVVISESRAHPEIGQLYLDNVIRHGLPLFEGLIARGVAAGEFRSLD
                     PSLAAKSLISPMLLAAIWKTVFEPLGAETLDIEGLARQHVDILLKGMAP"
     gene            1837..2805
                     /locus_tag="AC16A0_01475"
     CDS             1837..2805
                     /locus_tag="AC16A0_01475"
                     /inference="COORDINATES: protein motif:HMM:NF052712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3104027.1"
                     /translation="MKRFIPVVVVALAVTMFWYRDLWLPQPAGRASYLGYVEGETILM
                     AAPIAGRVVSRPVERGALVAAGTPVFSLDRAVAEADVSRAEAALAAAAASYDNLLTGK
                     RAPELAQIEAQRREAEANLALARKELMRARTLANSGTAAQSRLDTAEADVTRFEMRVA
                     QADASADVAALPARDKEIEAARAHVAEAQSAVAAARHKLSDLEPRAPRSARVEDTFFE
                     VGEWVNAGQPVVSLLPPEAVKLRFFVPEPVVALAQPGTTVTFTCDGCGEARQAVITSV
                     ASVPEYTPPVIYSQEARSKLVFRVEARPASPSGLLPGLPVAVAPLS"
     gene            2802..3731
                     /locus_tag="AC16A0_01480"
     CDS             2802..3731
                     /locus_tag="AC16A0_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104028.1"
                     /translation="MIAATAIDVKDLVKTYGSRTVVDHVDLRLAPGRICGFLGPNGSG
                     KTTTLRMICGLLTPDGGSGTCLGHDIILERDAIKRQTGYMTQRFGLYEDLTIRENLEF
                     VARLYRLDHLRSRVDRSLERLGLATRQHQLTGSLSGGWKQRLALAACVLHEPKLLLLD
                     EPTAGVDPKARRTFWDEIHALAGQGITVLVSTHYMDEAERCHEIAYIAYGRLMARGSS
                     SEIISQSGLKAVVATGPGADRLAPLLRDAPGITAAAAFGTTLHVCSTDEAVLDRAIAP
                     YRSNGIAWKREAPTLEDVFIHLMGQAQDNALAA"
     gene            3733..4860
                     /locus_tag="AC16A0_01485"
     CDS             3733..4860
                     /locus_tag="AC16A0_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104029.1"
                     /translation="MEFLARLWAMIVKEALQLKRDRLTFSMMFGVPIMQLVLFGFAIN
                     NDPKALPTAILSSDQSSITRAMIQAIENTSYFSAIHMAASEAEADELLQRGEVQFVIT
                     IPGDFTRRIMRGEVAQIAVEADATDPATTGPALSAVNQAVTQALGQELSGPMAYLKPQ
                     AAPVEIVIHRRYNPEGLTRYNIVPGLLGIILTMTMVMMTALAVTRERERGTMENLLAM
                     PVRPIEVMAGKIAPYILIGTVQVMVVLAAARVLFDVPFVGSYGLFCAATLLFITVNLA
                     IGFTFSTIARNQLQAMQMSFFFIMPSILLTGFAFPFRGMPGWAQAIGEAMPATHFLRV
                     VRGVMLKGADLQQMSPEIAVLALTLVIVAAVAVSRYKVTLD"
     gene            complement(4869..>5719)
                     /locus_tag="AC16A0_01490"
     CDS             complement(4869..>5719)
                     /locus_tag="AC16A0_01490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3104030.1"
                     /translation="RDFPAWHFPMVNDEARNAAIEAAIARLDLAGKTVVEIGTGTGLI
                     ALLFAKYGAARIVTCEINANLAAVAGRIVGATPYAGRIEIINDSSTNAIGRGLLPIEP
                     DVIFTETIDCGVVGEGFFSVAHDIRRLAGPRTIVLPSVVTQCIALVQSEEIANLNRAG
                     SACGFDLRALNDFSTLNYFPVRSELFNHDFLSKELQYRNFHYLNCPEPEIHKLNVLQS
                     GTVHGVLSWFTADFGGASVSNAALTGSHWHQAFHPLCEDMHVQEGDVVSMLIDDEGFA
                     WAVREG"
BASE COUNT         1021 a         1788 c         1820 g         1090 t
ORIGIN      
        1 catcctctgc tacccgcgca tcggcgaatg ctacaaatat gggcagtatt cggcaaagtg
       61 gacagcaaag atctatgggt attgaggaga aggcaatgat gaagcgcttt ggagttgcaa
      121 ttgcctcggc cgtcgcggct gctgccctgt tttcgctgcc tggactcgcc caggatgccg
      181 aaagggccaa gatgaccacc gaaatggacc tgcatgtcac agatctcgcc gatggcatgc
      241 tcagccccta tctggacaag gacgagcaga cactgatgaa atcgctggcc taccagcatg
      301 ccgttgcgtt gaactgcaaa ggcctctcgg tcgatcaggc gaaatcccag gcccagcttg
      361 agcgcgtgtt cccgcaggcc gagatggaca agatgacgga ggagcagcag ctcgccatga
      421 ccacatcggt catgatgggg ttcagcgcgg tgatgggtgg ccagttcgcc atcgctgcgg
      481 tggactacga tgcgtactgc aaggcggccg aggcggactt cgcctccaag gagggcaacg
      541 acacacacct gatcctcgtc aaggcgccgt gaggcgccag gcttgaacgc tctgctggat
      601 ccggcgtgat gagacttctc cgccgtcatc tcctgatcgc gccttatctg ttcttcacgc
      661 gcgccgctca cgccgatgac ggggcgtggg cggagctgcg tggcagtgag atcgtgctct
      721 tccgtcatgc caatgcgccc ggcactggcg atccgcctgg gttcgttgtc ggagactgct
      781 ccacccagcg caatctcgat gaacggggca gggtgcaggc gcgcgccatc ggtgaggcgt
      841 ttcgcaatcg gggcattcgc gtgggccggg tactgtcatc gcaatggtgc cgggcgcgtg
      901 acacagcgga gctggcattt cccggccagg tcaaccttga gccggcattc aattccttct
      961 tcggcaatcg cgagagcgag gaccgtcaga cggcggaggc gcagcgcgtg ctccgcgact
     1021 ggtctgggcc cggcgttctg gtggtcgtga cgcaccaggt caacatctcg gcgctcactg
     1081 gcatcgcacc caagtcgggc gaagggatcg tgcttcggct agcggacggg cagctcaccg
     1141 ttctcagtcg attgcccgca ccggatgcct aggggttgac agttactgac tgtcgggtta
     1201 gtaatcgcct atgactgacc agtcagtaac aaagaccagg cagcgccgtc ccgacgcgcg
     1261 tcccgccgag attcttggcg ccgcactgga actctttgcc gagaagggct tctcgggcac
     1321 ccgcctcgag gacgtggcgg cacgggcagg cctcagcaag gcggccatct atctctattt
     1381 cgatggcaaa atggacctgc tgcgggctct ggtgcaggag atggccggcg ccaacttggc
     1441 cgtcgcgggc agcatagcgc agggccatgt cggcgaggta gcgccgctcc tgcggcagat
     1501 cctcgtcttc atgtcgggac agctgcgcca cacgcgcttt ccggagttga tgaaggttgt
     1561 gatctccgaa agccgggccc atcccgagat cgggcaactc tacctggaca acgtgatccg
     1621 ccacgggctg ccgctcttcg aggggctgat cgcccgcggc gttgcggccg gcgagttccg
     1681 cagcctggac ccgtcgctcg ctgccaagtc gctgatctct ccgatgttgc tggcagcgat
     1741 ctggaaaacc gtcttcgaac ccttgggcgc cgagacgctg gatatcgagg ggctggctcg
     1801 tcagcatgtg gacatcctgc tgaagggaat ggcgccatga aacgcttcat tcccgtggtg
     1861 gtcgtggcct tggccgttac gatgttctgg taccgcgatc tctggctgcc ccagcctgca
     1921 ggccgggcga gctatcttgg ttacgttgaa ggcgagacga tcctcatggc agcgccgatt
     1981 gcggggcgcg tcgtgtcgcg tcccgtcgaa cgcggcgctt tggtcgcggc aggcactcct
     2041 gtcttctcgc tggatcgcgc ggtggcggag gccgacgtgt cgcgcgctga agccgcgctg
     2101 gcagccgctg cggccagtta cgacaatctg ctcaccggca aacgggcacc cgaacttgcg
     2161 cagatcgaag ctcaacggcg cgaagccgaa gcgaacctgg cgctggcacg caaggagctt
     2221 atgcgagcgc gcacgctggc caactcgggc acagccgcac agtcgcgcct ggacacggcc
     2281 gaagcggacg tcacgcgctt cgagatgcgg gtggcccagg ccgatgcctc ggccgatgtt
     2341 gcagcgcttc cggccaggga caaggaaatc gaagcggccc gtgcccatgt ggcggaagcg
     2401 caatcggccg tcgccgccgc gcgccacaag ctgagcgacc ttgagccgcg tgcgccgcga
     2461 tcggcgcggg tcgaggacac gttcttcgag gtgggcgaat gggtgaatgc gggccagcct
     2521 gtcgtgtcgc tcttgccgcc ggaagccgtg aagctccgct tctttgttcc cgagcccgtt
     2581 gtagccctcg cacaaccagg cacgacggtg accttcacct gcgacggatg cggtgaagcg
     2641 cggcaggcgg tgatcaccag cgtcgcctct gttcccgagt acacgccgcc agtcatctat
     2701 tcgcaggagg cccggtccaa gctggttttc cgggtcgagg cgaggccagc atcgccctcg
     2761 ggcctgctgc ccgggctgcc ggtcgccgtg gcgccattgt catgatcgct gcgacggcca
     2821 tcgacgtgaa ggatctggtg aagacctatg ggagccggac tgttgtcgat cacgtcgacc
     2881 tccgccttgc gccgggtcgc atctgcggct ttctcggtcc caacggttcc ggcaagacga
     2941 cgacgctgcg catgatctgt ggcctgctga cacccgatgg cggcagcggc acctgcctgg
     3001 ggcacgacat cattctggag cgcgatgcca ttaagcgcca gaccggctac atgacccagc
     3061 gcttcggact gtatgaagat ctcaccattc gcgagaatct cgaatttgtc gcgcggctct
     3121 atcggctcga tcatctccgc agccgcgtcg accggtcgct tgagcggctg ggccttgcca
     3181 cacgccagca tcagcttacc ggatcgcttt ccggcggctg gaagcagcgg ctggcccttg
     3241 cggcctgcgt gctgcatgag ccaaagctcc tgctgctcga tgaacccacg gcgggcgtcg
     3301 atcccaaggc gcggcgaacc ttctgggacg agattcatgc cttggccggg caggggatta
     3361 ccgtgctggt gtccacccac tacatggatg aagccgaacg ctgccatgag attgcctaca
     3421 tcgcatatgg ccgcctcatg gcgcgcggga gttcgtccga gatcatctcg cagtccggtc
     3481 tcaaggcagt ggtggcgaca ggccctggcg cggacaggct tgcacccctg ctgcgcgacg
     3541 caccgggcat cactgccgct gcggccttcg gtacgaccct tcacgtctgc agtaccgatg
     3601 aggccgtgct cgaccgcgcc atagccccct accgcagcaa cggcattgcg tggaaacgcg
     3661 aggcgccgac gctggaggat gtcttcattc acttgatggg gcaggcgcag gacaacgcgc
     3721 tggcagcctg acatggaatt cctcgcaagg ctctgggcca tgatcgtcaa ggaggcgctg
     3781 cagctcaagc gcgaccggct gaccttcagc atgatgttcg gcgtgccgat catgcagctg
     3841 gtgctcttcg gtttcgccat caacaacgac ccaaaggcgc tccccaccgc gatcctctcg
     3901 tcggatcagt cgtccatcac gcgcgccatg atccaggcga tcgagaacac aagctatttt
     3961 tcggccatcc atatggcggc aagcgaggcg gaagcggatg agttgctgca gcgtggcgag
     4021 gtgcagtttg tcatcaccat tccgggtgac ttcacccgcc gcatcatgcg cggggaggtg
     4081 gcgcagattg cggtcgaggc cgatgccacc gatccggcca caacaggccc ggcgttgtcg
     4141 gcggtcaacc aggccgtgac acaggcgctc ggtcaagaac tctccggccc catggcatat
     4201 ctcaagccgc aggccgcgcc ggtcgagatc gttatccacc gccgctacaa tccggagggt
     4261 ctcacgcgct acaacatcgt accgggcctt ctcggcatca tactgacgat gaccatggtc
     4321 atgatgacag cccttgccgt tacccgtgaa cgtgaacgcg gcacgatgga aaaccttctg
     4381 gccatgccgg tacgcccgat cgaagtgatg gccggcaaga tcgcacccta catcctgatc
     4441 ggaaccgtgc aggtgatggt tgttcttgca gcggcccggg tgctgttcga cgttcccttt
     4501 gtcggtagct atggtctgtt ttgcgctgcc accctgctgt tcatcaccgt caacctcgcc
     4561 atcggcttta ccttctcgac catcgccagg aatcagctgc aggcgatgca gatgtcgttc
     4621 ttcttcatca tgccctcgat tctcctcact ggctttgcct tcccctttcg gggcatgccc
     4681 ggctgggcgc aggcgatcgg cgaagccatg ccggccacgc actttctccg tgtggtgcgg
     4741 ggtgtgatgc tgaaaggtgc agaccttcag cagatgtcgc cggagatcgc tgtgctggcg
     4801 ctcactttgg tcatcgtcgc cgcggtggcc gtgagccgat acaaggtgac gctggactag
     4861 accgtcgcct atccctcgcg cacggcccag gcgaagcctt cgtcatcgat gagcatcgaa
     4921 acgacatcgc cctcctggac atgcatgtct tcgcagaggg gatgaaaggc ctgatgccag
     4981 tggctgcctg tcagcgccgc attggagacc gatgccccgc cgaagtccgc ggtgaaccag
     5041 ctgagcacgc catggacggt accggactgc agtacgttca acttgtgaat ctcgggctcc
     5101 ggacagttga gatagtgaaa gttacggtac tgcagctcct tgctcaggaa gtcatggttg
     5161 aaaagctccg accgcacagg aaagtagttg agcgtggaga agtcattcag cgcgcggaga
     5221 tcgaagccgc aggcgctgcc ggccctattc aggtttgcga tctcttcgga ttggaccagg
     5281 gcaatgcact gggtcaccac cgagggcagc acgatggttc tcggtcctgc aagtcggcgg
     5341 atgtcgtgcg cgacagaaaa gaatccctca cccaccacgc cgcagtcgat ggtctcggta
     5401 aagatcacat cgggctcgat tgggagcagg ccacggccga tggcattggt cgaactgtcg
     5461 tttatgatct cgatgcgacc ggcataaggc gtggcaccga cgatgcggcc ggccacggca
     5521 gcgagattcg cgttgatctc gcatgtcacg atccgcgccg cgccgtactt ggcaaagagc
     5581 agtgcaatga gaccggttcc tgttccgatc tcgacaacgg tcttgcccgc gaggtccagc
     5641 cgcgcgattg ccgcctcgat tgcggcgttg cgggcttcgt cattgaccat tggaaaatgc
     5701 caggcgggaa aatctctgc
//
LOCUS       DBNGVR010000069         2188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1203395, whole genome shotgun sequence.
ACCESSION   DBNGVR010000069 DBNGVR010000000
VERSION     DBNGVR010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1203395"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="AC16A0_01495"
     CDS             complement(<1..334)
                     /locus_tag="AC16A0_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104031.1"
                     /translation="MNTINRRKLLCRPRGGLNDMVSQIERCCRYAELTNRAVIVDTDY
                     GADSFGDDFDRYFISRQQHLILSAKGLDHELDCASTFPPSLARRMSSYTSSWDQRKDG
                     FVERESGEL"
     gene            complement(626..1114)
                     /gene="rpsI"
                     /locus_tag="AC16A0_01500"
     CDS             complement(626..1114)
                     /gene="rpsI"
                     /locus_tag="AC16A0_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3104032.1"
                     /translation="MAEQTTLADLGKAMGMATAAQAAAAAPAKPKLDAKGRAYATGRR
                     KDAISRVWVKRGSGKITVNGKAIETYFARPVLRMLVQQPLVLANRATQFDVECTVTGG
                     GLSGQAGAVRHGLSRAMTYFEPELRGVLKKAGFLTRDSRAVERKKYGRAKARRSFQFS
                     KR"
     gene            complement(1118..1582)
                     /gene="rplM"
                     /locus_tag="AC16A0_01505"
     CDS             complement(1118..1582)
                     /gene="rplM"
                     /locus_tag="AC16A0_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3104033.1"
                     /translation="MTTYTAKAKDIVKDWVLIDAEGLIVGRAAAIIANRLRGKHKPTF
                     TPHMDCGDHIVVINAEKIVLTGRKNQQKTYYWHTGFPGGIKERKAGKILEGAHPERVL
                     EQAVKRMLPGGPLKRQQMTHLRIYKGAAHPHEAQSPKKLDIRAMNPKNAVRG"
     gene            1844..>2188
                     /locus_tag="AC16A0_01510"
     CDS             1844..>2188
                     /locus_tag="AC16A0_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104034.1"
                     /translation="MRVFVFLLVSLVSTAAVQASVGSGSGTADMLAQAEAPLAPPPLP
                     DTGSREPPAGEAPSTLGDPPTIQDDPEVDLTAPDPNASEDDGSSPDNLSIGDIPVIET
                     MEITPEIARKALD"
BASE COUNT          371 a          710 c          696 g          411 t
ORIGIN      
        1 acagttctcc agactcccgc tcgacaaaac catccttccg ttgatcccag ctgctcgtgt
       61 agctcgacat gcgtcgggcc aggcttggcg gaaaggtcga agcgcaatca agctcgtgat
      121 cgagtccctt cgcgctgagg atcaggtgct gctgccggga aatgaaatag cggtcgaaat
      181 cgtcgccgaa gctatcggcg ccgtagtcgg tgtccacgat caccgcacgg ttcgtcaatt
      241 cggcgtaacg gcagcacctc tcgatctgcg acaccatgtc gttcagaccg ccacgcggcc
      301 ggcacagcag cttgcgccta ttgatggtgt tcactggcct tgcttccaca gatgctcaat
      361 ggtgcccgta tcggatgacg tcagtttgcg caacaggcag gcaaatggga agcatgccga
      421 gcggcaggcc ttgacgggaa ccgataacag gaatatattc tgtcgcacga tgagggagcc
      481 cagactggcc gaagcttgca actcgctcca gcatcagctg tatccggcaa ccactgccgt
      541 cgcccgccaa gcgcgccaga acggcaaagg ggcacctgcg atcgctcgca gatgccccgg
      601 aatgcgtcgg caaatgccgc ggcgcttagc gcttcgagaa ctggaagctg cggcgggcct
      661 tggcccggcc atacttcttg cgctcgacgg cgcggctgtc gcgggtcagg aacccggcct
      721 tcttgagcac gccacgaagt tcgggctcga agtaggtcat ggcgcgcgac aggccatggc
      781 gcaccgcgcc ggcctggccg gacaggccgc cgccggtaac ggtgcattcc acgtcgaact
      841 gcgtggcgcg gttggccagc accagcggct gctgcaccag catgcggagc accgggcggg
      901 cgaagtaggt ttcgatcgcc ttgccgttga cggtgatctt gcccgagccg cgcttgaccc
      961 agacgcgcga gatggcgtcc ttgcggcggc cggtggcata ggcgcggccc ttggcgtcga
     1021 gcttgggctt tgcgggcgcg gcagctgcgg cctgggcggc ggtcgccatg cccatggcct
     1081 tgccgaggtc ggcgagagtt gtctgttcag ccatgtctta tcccctcacc gcgttcttcg
     1141 ggttcatcgc ccggatgtcg agcttcttcg ggctctgggc ctcgtgcgga tgcgcggcgc
     1201 ccttgtagat gcgcagatgg gtcatctgct ggcgcttcag cggcccgccg ggcagcatgc
     1261 gcttgacggc ctgctcgagc acgcgctcag ggtgcgcgcc ttccaggatc ttgcccgcct
     1321 tgcgttcctt gatgccgcca ggaaagccgg tgtgccagta ataggtcttc tgctggttct
     1381 tgcgtccggt cagaacgatc ttctcggcat tgatcacgac gatgtggtcg ccgcagtcca
     1441 tgtggggcgt gaaggtgggc ttgtgcttgc cacgcaggcg gttggcgatg atggcggccg
     1501 cgcggccgac gatcagacct tctgcatcga tcagcaccca gtctttcacg atgtccttgg
     1561 ctttcgccgt ataggttgtc atctgaggtg tctctggtgt tgtggttcaa aggaaaaggc
     1621 gccggaggca agcccgcggc acccgtttca gtggccgctg gataggcttc tggacgaacc
     1681 ctgtcaagaa aaacagccaa aacacgcaac aaattggatg cggtatcatg ttacctcact
     1741 cagccgccgc aatcccgaga tgcctccgcg gcagggccgc aatgcccctt ccgtgacgtc
     1801 ccgccttgcc ctagtgtcga gcgagcgcca aggacgccct cgcatgcgtg tgttcgtgtt
     1861 tctgctcgtg agccttgtct ctaccgccgc cgtgcaggcc tcggtgggga gcggttctgg
     1921 cacggcggac atgctggccc aggccgaggc gccccttgcc cctcccccgc tgcccgatac
     1981 cggcagccgc gagccgcccg ccggcgaggc accgtcaacg cttggcgacc ccccaacgat
     2041 ccaggacgat ccggaggtcg acctcaccgc acccgatccc aatgcctccg aagacgacgg
     2101 cagctcgccc gacaatctca gcatcggcga catcccggtg atcgagacga tggagatcac
     2161 cccggaaatc gccaggaagg ccctggac
//
LOCUS       DBNGVR010000070         7883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1203830, whole genome shotgun sequence.
ACCESSION   DBNGVR010000070 DBNGVR010000000
VERSION     DBNGVR010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1203830"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16A0_01515"
     CDS             <1..294
                     /locus_tag="AC16A0_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR3104035.1"
                     /translation="RSHLTARHEDVIAFVSEEDFDIVGFSLSAERLMEPLVSVISEIR
                     RKSVNRQIRVMVGGVLFVGRPDLVKQSGTDVYVLDAREAVLQANEWCRSAKVN"
     gene            386..1774
                     /gene="ppsR"
                     /locus_tag="AC16A0_01520"
     CDS             386..1774
                     /gene="ppsR"
                     /locus_tag="AC16A0_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010109 - regulation of photosynthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PpsR"
                     /protein_id="HIR3104036.1"
                     /translation="MAGFDASNAAMILSAAADITFVVNADGIVSDRVMTSPEMPEELF
                     IGWPGKALVDLVTLESRPKVEQMLRDVAIGAPGRWRQVNHPTGSSEDLPIRYFAMRSG
                     TTGHAVLVGRDMRHLAAMQQRLLAAEQSIEQEYARLRHAETRYRLLMQVASEAILVVE
                     ARTGRIVEANPAAASLLSKPVKRLQGAVFQDIFDGTDAERIEGLLMSLRSTGRADDVA
                     LTLQGLPQQAVASSSVFRQENASYFLIRLQPLATGAGGIVVPKAQSAVLKIIQDLPDA
                     FVVTDADRRVLIANSAFLDSAQLGSEEQARGEPLDRWLGRAGVDTNLLFNSLAEKGSI
                     RQFQTVVRGQFGTSEDVEVSGVAALDAQPPCYGFSIRRVGRRPVETDGGRRSMLRSVD
                     EMTKLVGKVPLRDLVRETTDIIERLCIEAALQINDNNRAGAADMLGLSRQSLYVKLHR
                     YGIEDKSSGNEN"
     gene            1833..2747
                     /gene="chlG"
                     /locus_tag="AC16A0_01525"
     CDS             1833..2747
                     /gene="chlG"
                     /locus_tag="AC16A0_01525"
                     /EC_number="2.5.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthase ChlG"
                     /protein_id="HIR3104037.1"
                     /translation="MENSVSTRSGPALPTASAVVELLKPITWFAPMWAFGCGIVSSGA
                     PILSQWHLLLFGVALSGPLVCGTSQAVNDWFDRHVDAINEPGRPIPSGRVPGRWGLYI
                     AIIWSILSALVAALIGPVVLAAAIFGLAMAWAYSAPPLRLKRNGWWGNSAVGLCYEGL
                     PWFTGAAAMVGGVPDSRIILIALLYSIGAHGIMTLNDFKSVEGDRQMGLKSLPVQLGV
                     SGAARLACAVMAIPQLVVIGLLWQWDQPHHALAVTGLLVAQFALMNRLLSDPKGQASW
                     YNATGTTLYVLGMLVSAFALRGLAAVAS"
     gene            2744..4087
                     /locus_tag="AC16A0_01530"
     CDS             2744..4087
                     /locus_tag="AC16A0_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HIR3104038.1"
                     /translation="MTGAGLGWFGIVRLGLVQTALGAIVVLTTSTINRVMIVELALPA
                     MIPGLLVTWHYALQVLRPRWGHGSDGGGRRTRWIIAGMAALALGGVGAALSVVVMAYS
                     TALGMLIAAIAFTFIGGGVGAAGTSLLVLLAKSVREARRAAAATIVWIMMIMGFIVTA
                     GTAGHFLDPFSPSRLLVIATVICGVAFLLTLIAVWGVETSAGVEAAAKPEAAKVPFMQ
                     ALSQVWDDATARRFAAFIFVSMLAYSAQDLILEPFAGLVFGFTPGQSTSLSGLQNAGV
                     LLGMILVGAAATMWSRNKAMSLRNWTVAGCAGSALALAGLVAAAQVGPGWPLKPSVFA
                     LGFCNGVFAVAAIGSMFNLATSGTTSREGLRMGLFGASQAIAFGLGGFAGTVMVDVTR
                     MIYGSPVTAYAFVFAIEALLFIAAAIMAANIGRQEGTGHAVNPLILAARHQSGGN"
     gene            4089..5276
                     /locus_tag="AC16A0_01535"
     CDS             4089..5276
                     /locus_tag="AC16A0_01535"
                     /EC_number="1.3.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl diphosphate reductase"
                     /protein_id="HIR3104039.1"
                     /translation="MRETVTFDIAVIGGGPSGATAAHDAACLGLRVLLLDKAGRIKPC
                     GGAIPPRLIQDFEIPDHLIVAKVSSARIISPTDRKVDMPIDGSYVGMVDRDVFDEWLR
                     DRAAKAGAERRTGTYESLDRDGYGMPIVTYKDKQGRQESVRARIVIGADGALSKVARD
                     TVRGAERMPYVFAYHEIIRSPSPADAEFGPERCDVYYQGKLSPDFYAWVFPHGKTTSI
                     GTGSANKGFSLRDSVGELRQATGMASSETIRREGAPIPLKPLKRWDNGRDVVLAGDAA
                     GVVAPASGEGIYYAMLGGRLAAEAAAACLQTGDARQLATARKRFMKDHGKVFWVLGMM
                     QHFWYRNDKRRERFVSMCRDKDVQRLTWAAYTKKELVRSSPMAHMRIFVKDMAHLLGI
                     VPP"
     gene            5282..5761
                     /locus_tag="AC16A0_01540"
     CDS             5282..5761
                     /locus_tag="AC16A0_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIR3104040.1"
                     /translation="MSPFLTALAVSAGVAIVVAVAGGLLTELSPWYDRLKKPSWKPPD
                     WAFGPIWTIILILAAVSAALAWDAAPDTAMRGWILFVLIVNSILNIAWSGIFFKMKRP
                     DLALIEVLLLWLSIVALIVVLGQASRLAGLLMVPYLAWVSTAAFLNYRIVQLNRPFG"
     gene            5767..6102
                     /locus_tag="AC16A0_01545"
     CDS             5767..6102
                     /locus_tag="AC16A0_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104041.1"
                     /translation="MEELIRSGRIAHVIIAILLIELVVVSVYLLRRGRTREVLSAGAS
                     IIAGGALVMALGLALAGGGWKEIGVCLLISLVAHLAELVIRLSRQDAGSNRSGIGPTL
                     QDRNRETST"
     gene            6099..6476
                     /locus_tag="AC16A0_01550"
     CDS             6099..6476
                     /locus_tag="AC16A0_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3104042.1"
                     /translation="MKTTAAAALAALLAFAAPALADGDPVKGKAVFNKCKACHENEKG
                     VNKVGPTLKGVVGRKAASVPDYKYSEAMLAKGAEGVVWDEATLTAYLPDPKAFVPKTK
                     MAFAGLKKPEEIVDIIAFLKANP"
     gene            6487..7503
                     /gene="fni"
                     /locus_tag="AC16A0_01555"
     CDS             6487..7503
                     /gene="fni"
                     /locus_tag="AC16A0_01555"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR3104043.1"
                     /translation="MSEIERRKRDHIGIVLSGKARHSRAAGFDEIAFRHNALPDCDYA
                     EIDLSTAFLGRQLRLPYLASSMTGGPEVSEGINRAIAEAAQEMGFALGVGSQRIALTT
                     GESHGLDQTLRKLAPDVPIYANLGAVQLVHGMGRDQAMRAVEQIGADALILHLNPLQE
                     ALQEGGDRNWRGVGAAIAQLARNFPLPIIVKEVGSGISAEVAQRLADCGVAAVDVAGA
                     GGTSWAAVEGRRAGDAAGQELGEVFRDWGIPTTDCLIEIHRVLPSLPLVASGGIRSGL
                     DGAKAIRLGATLVGQAGALLPAAMRGTEAVIMHVTEWATALRIACFATGSRNLGELRS
                     AALI"
     gene            complement(7515..>7883)
                     /locus_tag="AC16A0_01560"
     CDS             complement(7515..>7883)
                     /locus_tag="AC16A0_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104044.1"
                     /translation="VPVHAPFGRGRTALSILESFDPGDSGMPEAEHLPLWDEATPMSS
                     ALMMPVLLDDPAPLSKFWNIAPLASDSCYASGVIGMPLLSRSDSPGDSRWGLPFLSGN
                     PAPLSSMLGLPLLTKPKASW"
BASE COUNT         1318 a         2372 c         2630 g         1563 t
ORIGIN      
        1 cgaagccacc tcaccgcccg gcatgaagat gtgatcgcat tcgtttcgga agaggatttt
       61 gacatcgtgg ggttttcgtt gagcgcggag cgcttgatgg agcccctcgt gtccgttatc
      121 tcggagatca gacgcaagtc cgtgaatcgt caaatccggg tgatggtcgg cggtgtattg
      181 ttcgtcggac gcccggacct cgtgaaacaa agcggcactg atgtctacgt tctcgatgcc
      241 cgcgaagctg ttttgcaggc caatgagtgg tgcaggtccg cgaaggtcaa ctgacgtcgc
      301 gaacccttgg aaagtggata caatggaagc atcgcgtaga ggcgatccag cggcatccgg
      361 caaaggcacg agtcttgaaa ggctcatggc tggcttcgac gcgtcgaatg cggccatgat
      421 cctgtcggcg gcggccgaca tcacatttgt cgtcaatgcc gatggcatcg tcagcgaccg
      481 ggtcatgacg tcgccggaaa tgccggaaga gcttttcatc ggctggccgg gcaaggcact
      541 cgtcgacctt gtgacgttgg agagtcgtcc gaaggtcgag cagatgctgc gcgacgtcgc
      601 gatcggtgcg ccaggccggt ggcgccaggt gaatcatccg accggatcga gcgaagatct
      661 tccgatccgt tacttcgcaa tgcgcagcgg cacgacaggc catgccgttc tggtcggtcg
      721 tgacatgcgc catcttgcgg ccatgcagca gcgactcctg gcagccgagc agtcgatcga
      781 gcaggagtac gcgcgtctgc gccatgccga gacccgctat cgcctgctga tgcaggtagc
      841 ttcggaagcg atcctcgtcg tcgaagcgcg caccggtcgc atcgttgaag cgaatccggc
      901 ggcagcttcg ctgctcagca agccggtgaa gcgccttcag ggtgcggtgt tccaggacat
      961 tttcgacggt acggatgccg agcggatcga aggcctgttg atgagtctgc gctcaacggg
     1021 gcgtgccgac gatgtcgcgc tcacattgca gggtctgccg cagcaggccg tggcgagttc
     1081 gagcgtgttc cgccaggaga atgccagtta cttcctgatc cggttgcagc ctcttgccac
     1141 cggtgccggc gggatcgttg tgccgaaggc gcaatcggcg gttctcaaga tcatccagga
     1201 tctgccggat gccttcgtgg tcacagatgc ggatcgacgg gtgctcatcg ccaattcggc
     1261 ctttcttgac agcgcgcagc tgggcagcga ggagcaggcg cgcggcgagc cgcttgaccg
     1321 ctggctcggg cgcgcgggcg ttgacaccaa tctcctgttc aacagcctgg ccgagaaggg
     1381 gtctatccgc cagttccaga ccgtagttcg cggtcagttc ggcacgtcgg aggatgtgga
     1441 agtgtccggc gtcgccgctc tcgatgcgca gccgccctgc tatggcttca gcattcgccg
     1501 tgtcggccgg cggccggtgg agaccgacgg tggccgccgc agcatgcttc gttccgtcga
     1561 tgaaatgacc aagctcgtcg gcaaggtgcc gctgcgtgat ctggtgcgcg aaaccaccga
     1621 catcatcgag cgcctctgta tcgaggcggc gcttcagatc aatgataaca accgcgcagg
     1681 cgccgcggat atgctgggac tgagccgtca gagtctctat gtcaagctgc accgctacgg
     1741 cattgaagac aagtcttccg gcaacgagaa ttgacaattc aaactgtcat gtttgattga
     1801 caatcatttg ggccgcagat agtctttgcg gcatggagaa ctcggtttca acccgctccg
     1861 gcccggccct tccgactgct tccgcagtgg tcgaacttct gaagcccatc acgtggtttg
     1921 ccccgatgtg ggccttcgga tgcggcatcg tttcgtccgg cgctcccatc ctttcgcaat
     1981 ggcacctgct cttgtttggc gtggcgctgt cgggtcctct ggtttgcggg accagccagg
     2041 cggtgaacga ctggttcgac cgccatgtcg atgccatcaa cgagcccggt cggccgattc
     2101 cttccggccg cgttccgggc cggtgggggc tttacatcgc cattatctgg tcgatcctgt
     2161 ctgcattggt ggctgccctg atcggaccgg tcgtgctggc cgccgcaatc tttggactgg
     2221 ccatggcatg ggcctatagc gcgccgccgt tgcggctgaa gcgcaacgga tggtggggaa
     2281 acagcgctgt cggcctctgc tacgaaggcc tcccatggtt caccggtgca gcggccatgg
     2341 ttggcggcgt gcctgactcg cgcatcatcc tgatcgctct gctctacagt atcggcgcgc
     2401 atgggataat gacgctcaat gacttcaagt cggtcgaagg cgaccggcag atgggcctga
     2461 agtcgctccc ggtgcagctc ggggtctctg gtgcagcgcg tctcgcctgt gccgtcatgg
     2521 ccatccccca gctggtggtg attggcctgt tgtggcaatg ggatcagccg catcatgcgc
     2581 ttgccgtcac cggactgctg gttgcgcagt tcgccctcat gaaccggctc ctgtccgacc
     2641 cgaaggggca ggcctcctgg tacaacgcga cgggtacgac gctctatgtg ctcggcatgt
     2701 tggtgagcgc ctttgcattg cggggacttg cagcggtggc gtcatgacgg gcgcaggtct
     2761 tggatggttc gggatcgtca ggctgggcct ggtgcagacg gcgctgggtg ccatcgtggt
     2821 gctgacgacg tccaccatca atcgcgtgat gatcgtcgaa ctggctctgc ccgcgatgat
     2881 cccaggcctg cttgtcacat ggcattatgc attgcaggtt ctgaggccgc gctggggcca
     2941 cgggtcggat ggcggcggcc ggcggacccg ctggatcatt gccggcatgg cggccctcgc
     3001 gcttggtggc gtgggtgctg cgctctcggt cgtggtgatg gcctacagca cagccctcgg
     3061 catgctgatc gcggcaatcg ccttcacgtt catcggcggc ggtgttggtg cagccggcac
     3121 atccctgctt gttctcctgg ccaagagcgt ccgggaggcc cgcagggccg cggccgccac
     3181 catcgtgtgg atcatgatga tcatgggatt catcgtgacg gcgggtacgg ccggccactt
     3241 cctcgatccc ttttcgccca gccgcttgct ggtgatcgcc acggtcatct gcggcgttgc
     3301 cttcctcctc acgctcattg cggtgtgggg cgtggaaacc tctgcgggcg tcgaggccgc
     3361 ggcaaaacct gaagcggcca aggtcccctt catgcaggcg ctgtcacagg tctgggacga
     3421 tgccacggcc cggcgcttcg ctgccttcat tttcgtgtcc atgctggcct atagcgcgca
     3481 ggacctcatc ctcgagccat ttgccggcct ggtcttcggc ttcacgccgg ggcagtcgac
     3541 ctcactgtcc gggctccaga acgcaggcgt cctgctcggc atgatccttg ttggtgccgc
     3601 tgccaccatg tggagccgaa acaaggcaat gagcctccgc aactggacgg ttgcaggatg
     3661 cgcgggatcc gcgctcgcgc ttgccggttt ggtggcagct gcacaggttg ggccaggctg
     3721 gccgctgaag ccgtccgtct ttgcgctcgg cttctgcaat ggagtcttcg cggtggctgc
     3781 catcggctcg atgttcaatc tcgccaccag cggcacgacc tcgcgcgagg gcctgcgcat
     3841 gggactgttc ggcgcatcgc aggccatcgc cttcgggctt ggcggattcg ctggcacggt
     3901 catggtcgat gtgacccgca tgatctatgg atcgccagtc acggcctatg ccttcgtctt
     3961 tgccatcgag gcacttctct ttattgcagc ggcgatcatg gccgcgaaca tcggaaggca
     4021 ggaaggcaca ggacatgcgg tgaatccgct gatccttgcc gcacgccatc agtctggagg
     4081 aaattgacat gcgggaaacc gtcaccttcg atatcgccgt gatcggcggc gggccatcgg
     4141 gcgctacggc tgcgcatgat gcggcctgtc tgggcctccg ggtgctgctg ctcgacaagg
     4201 ccgggcggat caagccctgt ggcggtgcaa ttcccccgcg gctcattcag gacttcgaga
     4261 ttcccgatca tctgatcgtg gccaaggtca gcagtgcccg gatcatttcc ccgaccgacc
     4321 gcaaggtcga catgccgatc gacggcagct acgtcggcat ggtcgaccgc gatgtcttcg
     4381 acgaatggct gcgcgaccgt gctgcaaagg cgggtgcgga gcgccggacc ggcacctacg
     4441 agagtctcga tcgcgacggc tacggcatgc ccatcgtcac ctacaaggac aagcagggcc
     4501 ggcaggagtc ggtcagggca cgcatcgtga tcggtgcgga cggtgccctg tcgaaggtcg
     4561 cccgcgatac cgtgcgcggg gccgagcgca tgccctatgt cttcgcctac cacgaaatca
     4621 tccggtcgcc gtccccggcc gatgcggagt tcgggccgga gcgctgcgat gtctattatc
     4681 agggcaagct gtcgccagac ttctatgcct gggtgttccc gcatggaaag acgaccagca
     4741 tcggcaccgg cagcgccaac aagggttttt cgctgcgcga ttcggtcgga gaactgcggc
     4801 aggcgaccgg gatggccagc agcgagacga tccgcaggga gggcgctccc attccgctca
     4861 agcctctgaa gcgctgggac aatggccgcg atgtggtgct ggcaggagat gcggccggtg
     4921 tcgttgcgcc ggcatcggga gagggcatct attatgcgat gctcggagga cgtctcgccg
     4981 ccgaagccgc ggcggcgtgc ctgcagacgg gcgatgcgcg tcagcttgcg acggcccgca
     5041 agcgcttcat gaaggatcac ggcaaggtgt tctgggttct cggcatgatg cagcacttct
     5101 ggtatcgcaa cgacaagcgg cgcgagcgtt tcgtgagcat gtgccgcgac aaggatgtgc
     5161 agcgcctgac atgggcggcc tatacgaaga aggaactggt gcgttccagc ccgatggccc
     5221 acatgcggat cttcgtcaag gacatggcgc atctcctggg catcgtgccg ccgtgagttt
     5281 catgtcgcct ttcctgacgg ccttggccgt ctcggcaggc gttgcgattg tagttgccgt
     5341 ggccgggggg ctcctgaccg aactcagtcc ctggtatgat cgcctgaaga aaccttcatg
     5401 gaagccgccc gactgggcct tcggaccgat ctggacaatc atccttattc tggcggcggt
     5461 ctcggcggcc ctggcctggg acgccgcacc ggataccgcc atgcgtggat ggatcctgtt
     5521 tgtcctgatc gtcaacagca ttctcaacat tgcctggagc ggcatcttct tcaagatgaa
     5581 gcgccccgac ttggctctga tagaagtcct gctgctgtgg ctatcgatcg tcgcgctgat
     5641 tgtcgtgctg gggcaggcat cgcgtttggc gggcctgctc atggtgcctt atctcgcgtg
     5701 ggtttcgacg gccgccttcc tgaactaccg gatcgtccag ctgaaccggc ctttcggctg
     5761 acggtcatgg aagagctgat ccgcagtggt cgaatcgctc atgtgatcat tgcgatcctg
     5821 ctgatcgagc ttgttgtggt gtctgtttac ctcttgcgcc gcggacggac acgcgaggtc
     5881 ttgagtgcag gtgcttcgat catcgccggt ggggcgctcg tcatggcgct cggcctcgct
     5941 ctcgcgggcg gtggctggaa ggagattggt gtctgtctcc tcatcagctt ggttgcgcat
     6001 cttgcggagt tggtcataag attgtcgcgc caggatgcgg gaagcaaccg ttccggcatc
     6061 ggaccaacgc tccaagacag aaaccgggaa acatccacat gaagacgact gcagccgcgg
     6121 cgctcgccgc actccttgcc tttgccgcac ctgctcttgc cgacggtgat cccgtgaagg
     6181 gcaaggcggt gttcaacaaa tgcaaggcct gccacgaaaa cgagaagggc gtgaacaagg
     6241 ttggtcccac gctcaagggt gtcgtcgggc gcaaggccgc ctcggtcccc gactacaagt
     6301 attcggaagc catgcttgcc aagggcgcgg aaggcgtcgt gtgggacgag gcgaccctta
     6361 ctgcctatct gcccgatcca aaggccttcg ttccgaagac caagatggcc tttgccgggc
     6421 tgaagaagcc ggaggagatc gtcgacatca tcgccttctt gaaggccaac ccctgatgcg
     6481 aggccgatga gcgagatcga gaggcgcaag cgcgaccaca tcggcatcgt gttgtccggc
     6541 aaggcacggc acagccgtgc tgcgggtttt gacgagatcg cattccggca caatgcgctg
     6601 ccggattgcg actacgcgga gattgatctc tccaccgcgt ttctggggcg acagcttcgg
     6661 ttgccctatc tggcgtcttc gatgacgggt gggccggagg tgtccgaggg catcaaccgc
     6721 gccattgccg aggccgcgca ggagatgggc tttgccctgg gcgtgggatc gcagcgcatt
     6781 gccctgacca caggcgagag tcacggcctc gaccagacgc ttcggaaact ggcgcccgac
     6841 gtgccgatct atgcgaacct gggtgcggtg cagctcgtgc atggcatggg ccgggaccag
     6901 gccatgcgcg cggtcgagca gattggcgcc gatgccctga tccttcatct caatccgctg
     6961 caggaggcct tgcaggaggg gggcgaccgc aactggcgcg gcgttggggc cgccatcgcg
     7021 caactggcgc ggaacttccc cttgccgatc atcgtgaagg aggttggcag cggaatatcg
     7081 gcagaggtcg cgcagcggct ggccgattgt ggcgttgcgg ctgtagatgt cgccggggcc
     7141 ggcggtacga gctgggctgc cgtcgaagga cgaagagcag gggatgccgc aggccaggaa
     7201 ctgggcgagg tcttccgcga ctggggcata ccgactacgg attgcctcat cgagatccat
     7261 cgggttttgc ccagcctgcc gctcgttgcc tcgggcggca ttcgcagcgg gctggatggc
     7321 gccaaggcca ttcgcttagg ggccacgctg gtcggccagg cgggagcgct tctcccggcg
     7381 gccatgcgcg gcaccgaggc ggtgatcatg catgtgacgg aatgggccac agcgctccgc
     7441 atcgcctgct ttgcaacagg ctcgcgcaat cttggcgaac tccggtcggc cgcgctgatc
     7501 taggccggtc ttgcctacca ggatgccttg ggcttcgtca gcaagggaag tccaagcatg
     7561 gacgacaagg gcgcgggatt gccgctcagg aagggtagac cccagcgcga gtcgcccggg
     7621 ctgtcggagc gggacagcag cggcatgccg atcacgcccg acgcgtaaca ggaatcgctg
     7681 gccaggggcg cgatgttcca gaactttgac aagggcgccg ggtcgtcaag cagcacgggc
     7741 atcatcaggg cggaggacat gggggtcgcc tcgtcccaga gcggcagatg ctcggcttcc
     7801 ggcatgccgg aatcaccggg atcgaaggac tcgagaatgg acagtgccgt gcggccgcgc
     7861 ccaaagggcg cgtgaaccgg cac
//
LOCUS       DBNGVR010000071         3704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3167638, whole genome shotgun sequence.
ACCESSION   DBNGVR010000071 DBNGVR010000000
VERSION     DBNGVR010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3167638"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..>3704
                     /locus_tag="AC16A0_01565"
     CDS             356..>3704
                     /locus_tag="AC16A0_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HIR3104045.1"
                     /translation="MIVAAAFYIRLANGPVSLDFMTATFQEQINKNLSGMKVKIDGVI
                     IERAPESGVPHFRLSKIELLDTAGNLIARAPRAAIGINERALFSGRVVPVSLELIGPR
                     IQMKRNLDGKFELGFGAPAAEESQAVVVGDEPASTGAKSDQEGIGGKAPLANNTQRAF
                     GESLISVLSGDGGQPTQGIGAIETVRVTDAAIKFYDEANDAIWDAPKAELAFKRMPYG
                     FAVVANADVANGNETGNWRTEISASYRRDSKSFAISARIDDLVPANISDEIFALAQLA
                     RFKVPLSGHAEMEINEDGTLTKASAEFSAAAGEVNFPDYLAEPLIVDEGSLRIDFDPP
                     TGGIVITDSQLLVGSSRAQITGNILPVRSAEGLLSAVNISLNARNVAIDTEGTVVSPV
                     AVDRIDFVGSAAINEARLDIEDLVIMSGSAGIRVKGAITGGGESVGLLLSGRMRDLSA
                     DLLKRLWPPIMVPKTRDWVSKNVIDGTVTDGEFIVNLPVDALATAKREKRLPPNSIDL
                     HFRMADVSTRYFKELPPLIKASGEAWLKDDDFRLEMTSGEIVLPSGKLGKLDKGTMAA
                     QDIMVPETVAQYTFDVTAPTQAILEFAGHPSLNLIKKSGFDTTKLMGNAKLSGKLAVP
                     MMKGPPPERVIFSAEAKVRDAALEGALPGIDITDGVVGVTVEKGALEAKGPVKINGVP
                     VELTWRRAVGPGGKQSAVIEATLDGEERKKIGVDLGEFLRGPMAVKADLADLADPEGR
                     IDIEADLSKSEMQIAAISWSRPPVLKTSATLVYFNKGDRGRRIEDLVIKGEGLFIRGD
                     VSLLPKGGMKEARLSEVKLSDENRFALTMTNGESGSTIKINGDSFDARPLIRSMFGAT
                     RPGGSDDSLSKRTPLSITVNLDRVYAHRGEILTNVSGTIRTRGSTVEAAEVTGAFLSG
                     QPVVLRVTPDGDGREMRISGRDAGAAMRAANLYSKIAGGQIEFFAMMDNDASSSVREG
                     KLVIRDFEVRNEAALAELDAKGRPKKSGPRKDGLAFKRLTLPFTTDARFLRLGDSLVR
                     GNELGATAQGVIRKSDGAIDITGTIIPAYGPNAALGNIPLLGDILTGGGGEGIFGLTY
                     ALGGTMNKPVFQANPVSAIAPGIFRKLF"
BASE COUNT          738 a         1111 c         1203 g          652 t
ORIGIN      
        1 aagatcatct gttctccggt gactttgggg cggcaggacg aatcccgcag cgactcagca
       61 gaaggttaag gcgtgtgccg gaacctgtca tttgttaatg ctgggaacgg gcagaccggc
      121 cttgcaacag gtcctccgga cactacgatg cggccgcccc ccgcactttg gaacgagtcg
      181 ggccgggcgc ccggagaatg tcacgcttta gcccatgttt gatggccaaa ggagcggttg
      241 tcagggcgga gcgcgcttcg ccaaggcagg atcttgagga atgggctaga agagtttgcg
      301 gcgcaaaatc atagggataa cggcctacag catccttggg gtgctggcgg ccgtcatgat
      361 tgtggccgcc gcattctaca ttcggctggc caatgggccg gtgtcgctcg acttcatgac
      421 cgcaaccttc caggagcaga tcaacaagaa cctctcgggc atgaaggtga agatcgatgg
      481 cgtcatcatc gaacgggcac cggagagcgg cgtgccgcat tttcgcctga gcaagatcga
      541 acttctggac acggccggca acctcatcgc ccgcgccccc cgtgcggcca tcgggatcaa
      601 tgagcgagca cttttttccg gccgcgtcgt tccggtgtcg ctggaactaa tcgggccgcg
      661 catccagatg aagcggaacc tcgatggcaa gttcgaactc ggttttggtg cgccggcggc
      721 agaggaatcg caggcggtgg tcgtgggcga cgaacccgcc agcaccgggg caaaatcgga
      781 tcaggaagga atagggggga aggctccgct cgccaacaac acccagcggg cctttgggga
      841 gtcgctgatc tcggtgcttt cgggcgacgg cggacagccg acgcaaggca tcggcgccat
      901 cgaaaccgtg cgggtcacgg atgcggcgat caagttctat gacgaggcca atgacgcgat
      961 ctgggatgcc cccaaggccg agttggcctt caagcgcatg ccttatggct tcgcggtggt
     1021 tgccaatgcc gacgtggcca acggcaacga aaccggcaac tggcgaaccg agatctctgc
     1081 cagctatcgg cgagacagca agtccttcgc gatcagcgcc cgcatcgatg acctcgtgcc
     1141 cgcgaacatc tcggatgaaa tcttcgcgct ggcccaactg gcgcggttca aggttccctt
     1201 gtccggccat gcggagatgg aaatcaacga ggacgggacg ctgaccaagg cgtcggcaga
     1261 gttctccgct gccgcaggag aggtcaattt tccagactat ctggccgagc cgctgatcgt
     1321 cgacgaagga tcgcttcgga tcgacttcga tccgccaacg ggcggcatcg tcattaccga
     1381 ctcgcagttg cttgtcggca gctcgcgcgc gcagatcacc ggcaacatcc tgccggtgcg
     1441 cagtgccgag ggccttctgt ctgccgtgaa catttcgctc aatgcgcgca acgtggcgat
     1501 cgacacagag ggcacggtcg tgtcgcctgt ggctgtggat cgcatcgact tcgtcggcag
     1561 cgcagccatc aacgaggccc ggctggacat cgaggatctc gtgatcatgt cgggttctgc
     1621 gggcatccgg gtgaaaggcg caatcaccgg cggcggggaa tccgtcggac tgctgctctc
     1681 gggacgcatg cgcgatctgt cggccgatct gctcaagcgc ctgtggccac cgatcatggt
     1741 gcccaagacg cgcgactggg tcagcaagaa cgtgatcgac ggcacggtga cggacgggga
     1801 gttcatcgtc aatctgccgg tggatgcact cgccaccgcc aagcgcgaga agcgcctgcc
     1861 gccgaattcc atcgatctgc atttcagaat ggcggacgtg tcgacgcgct atttcaagga
     1921 gctcccgccg ctcatcaagg ccagcggcga agcctggctc aaggatgacg acttccggct
     1981 ggagatgaca tcgggcgaga tcgtccttcc gtcgggcaag ctcggcaagc tcgacaaagg
     2041 caccatggcc gcccaggata tcatggtacc cgagacggtc gcgcagtaca cgttcgatgt
     2101 cacggcgccg acccaggcca tactcgagtt tgccggccat ccgtctctca acctcatcaa
     2161 gaagtccggt ttcgatacca ccaagctcat gggcaatgcc aagctctccg gcaagcttgc
     2221 ggtgccgatg atgaaggggc cgccgcccga gcgggtgatc ttttccgcgg aagcgaaggt
     2281 gcgcgatgcc gcgctcgagg gcgctttgcc gggtatcgac atcacggacg gcgtagtggg
     2341 agtgacggtt gagaagggcg ctctggaggc aaaaggaccc gtcaagatca atggcgtgcc
     2401 ggtggagttg acctggaggc gcgcggtggg accgggcggc aagcagtccg ccgtgatcga
     2461 ggccacgctt gacggcgaag agcgcaagaa gatcggcgtg gatcttggcg aattcctgcg
     2521 cggacccatg gccgtgaagg ccgaccttgc cgaccttgcc gaccccgaag gtcgaatcga
     2581 catcgaagcc gatctgtcaa agtcggaaat gcagatcgcg gcgatcagct ggtcgcggcc
     2641 gccggttctc aagaccagcg caaccttggt ctatttcaac aagggcgaca gagggcggcg
     2701 catcgaagac ctcgtcatca agggagaagg cttgttcatc cgtggcgacg tcagccttct
     2761 gcccaagggc ggcatgaagg aggctcggtt gagcgaggtg aagctcagcg acgagaaccg
     2821 cttcgccctg accatgacca acggagagtc cgggtcgacg atcaaaatca atggcgacag
     2881 tttcgatgcg aggccgctga tcaggtccat gttcggcgcc acccggccgg gtggcagcga
     2941 cgacagtctg tcgaagcgca cgccgctcag catcacggtc aatctcgatc gcgtctacgc
     3001 gcatcgcggc gaaatcctca ccaatgtctc gggcacgatc cgcacgcgtg gcagtaccgt
     3061 cgaggccgcg gaggttaccg gagcctttct gtcggggcag cccgtcgttc tgcgcgtcac
     3121 gcctgacggc gatggccgcg agatgcgtat ctccggccgc gatgcggggg ctgcaatgcg
     3181 ggcggccaac ctctattcca agatcgccgg cggccagatc gaattcttcg cgatgatgga
     3241 caatgacgcc agttcgtcgg tgcgcgaagg caagctggtg atccgcgact tcgaggtccg
     3301 caacgaggca gcgctcgccg aactggatgc caagggcagg ccgaagaagt ccggaccacg
     3361 caaggacggc cttgccttca agcggctgac cttgccattc accacggatg ccagatttct
     3421 caggcttggt gactccctgg tgcggggaaa tgaactgggc gccactgcgc aaggcgtcat
     3481 ccgcaagtcc gacggcgcca tcgacattac cggaaccatc attccggcat acgggcccaa
     3541 tgctgccctg ggcaacatcc cgttgctggg cgatatcctg acgggtggtg gcggcgaagg
     3601 cattttcggc ctcacctatg cgctgggagg caccatgaac aagccggtgt tccaggcaaa
     3661 tcctgtgtcc gcaatagcgc cgggcatctt ccggaagctc ttcg
//
LOCUS       DBNGVR010000072         6493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2185895, whole genome shotgun sequence.
ACCESSION   DBNGVR010000072 DBNGVR010000000
VERSION     DBNGVR010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2185895"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16A0_01570"
     CDS             complement(<1..512)
                     /locus_tag="AC16A0_01570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543468.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3104046.1"
                     /translation="MDIRAAEISAILKKQIKDFGKEAEVTEIGQVLSVGDGIARVYGL
                     DNVQAGEMVEFANGMKGMALNLETDNVGIVIFGNDRDIKEGDTVKRTGAIVEVPVGKE
                     LLGRVVDPLGNPIDGKGPIKSKTFKRVDVKAPGIIPRKSVNEPMSTGLKAIDALIPIG
                     RGQRELVIGDR"
     gene            complement(512..1072)
                     /locus_tag="AC16A0_01575"
     CDS             complement(512..1072)
                     /locus_tag="AC16A0_01575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016736391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR3104047.1"
                     /translation="MASTDQTVTGVPGRYASALFDLAKEEKSVDSVQAQLAGFRAAIE
                     GSADLKRMVTSPVFTSADQIAAIEAICAKAKVTGTSLNFLRLVVKNRRLPALPAIIDA
                     FDTLVAISKGEIGAEVISADKLSAVQLKDLKAALKASTGRDVTLSTRVDSSLLGGLIV
                     KVGSRMMDNSLKTKLQNLKIAMKGTA"
     gene            complement(1321..1488)
                     /locus_tag="AC16A0_01580"
     CDS             complement(1321..1488)
                     /locus_tag="AC16A0_01580"
                     /inference="COORDINATES: protein motif:HMM:NF018294.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiS-like domain-containing protein"
                     /protein_id="HIR3104048.1"
                     /translation="MIGFSGLAQLLLRSRRMKAYRQARAEIEALSEADLADMGLKRYQ
                     LGHVARSKSLR"
     gene            complement(1485..1637)
                     /locus_tag="AC16A0_01585"
     CDS             complement(1485..1637)
                     /locus_tag="AC16A0_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HIR3104049.1"
                     /translation="MFEVLKSRYAVWKRYSRTVSELEMLSTRELADLGISRCDIPRLA
                     REAVRS"
     gene            complement(2007..3686)
                     /locus_tag="AC16A0_01590"
     CDS             complement(2007..3686)
                     /locus_tag="AC16A0_01590"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR3104050.1"
                     /translation="MSTVKSDIEIAREAKMKPIMEIGRGLGIPEKDLIPYGHTKAKVS
                     LDYIDSLKSKKDGKLILVTAINPTPAGEGKTTTTVGLGDGLNRIGKKAMIALREPSLG
                     PCFGMKGGAAGGGYAQIVPMEDINLHFTGDFHAITSAHNLLSAMIDNHIYWGNELGID
                     TRRVAWRRVMDMNDRALREIVCSLGGVANGFPREAGFDITVASEIMAILCLSTSIADL
                     ERRMGQIVVAAKRDKGQVLAKDIKAPGAMTVLLKDALMPNLVQTLENNPAFVHGGPFA
                     NIAHGCNSVMATQTALKLADYVVTEAGFGADLGAEKFFDIKCRKAGLTPAATVIVATV
                     RALKMHGGVAKDDLKKENVEALKKGLANLGRHIENVKKFGVPIAVAINHFISDTDAEI
                     EAVREYCKGHGVEAFKCTHWGEGGKGIEGLAKHVAELADKGAAQFKPIYDESLSLWDK
                     VRTIAKEIYRADDIVADKSVRDQLKAFEAQGYGKFPICMAKTQYSFSTDPNLKGAPTN
                     HVVPIREVRLSAGAEFIVVICGDIMTMPGLPKVPSAEVIGLDDKGQIAGLF"
     gene            3890..5467
                     /locus_tag="AC16A0_01595"
     CDS             3890..5467
                     /locus_tag="AC16A0_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3104051.1"
                     /translation="MRAFDRPGRSTAYSTSGMAATSAPMATLAAVECLKAGGNAVDAA
                     VTASAVLCVVEPAMTGIGGDCFALIGTPDGKVQGLNASGRSARAADADWLSASGLTRI
                     APRSIHAITVPGAIDGWDQLLRAYGTRSLADCLVPAIGMAEAGTPVTPRVALDWPEDA
                     PDLLADAGGQQHYLKNGLAPQEGDVIAYPALARTLRLIAREGRDAFYAGPIAADIVGH
                     VRAMGSLLTLADFSSHTSTWVEPISTGFAGHEVLEIPPSGQGLTALIALNVVSHFGLA
                     RHEPDSPDRMHIEIEAMKLAWELRNRHIADPDFAEVPVAELLSPGMAARLAGMIDMKQ
                     ALDIRTAMRQSDTIYLSVVDRNRLAVSFINSIYYGFGSAIVTPATGISLQNRGACFVA
                     DPGHPNCIGPAKRPLHTIIPAMSRKDGRVDMSFGVMGGDYQPMGHLCVAVNRYIYGMD
                     PQAAIDMPRYFPKGGEVLVESGVTEAAQAGLRDRGHALVPAPEPLGGGQAIVIDHRRG
                     ILIGGSDPRKDGLALGI"
     gene            5575..>6493
                     /locus_tag="AC16A0_01600"
     CDS             5575..>6493
                     /locus_tag="AC16A0_01600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3104052.1"
                     /translation="MSSDSSGNHHRSGTAKAADPLGSALRAMEAAGVATYNWVIDTDE
                     IVWSSNAADVMGCDPGEIATGRAFASYLDPDNFTSRYDAVMRTTVIDDGDGVPFQIEY
                     KFRPEGRLGRASLWLEDHGSWFGGKDGRPVEVFGTVRRIDARHQRDQHLSFLGNCDPL
                     TGMMNRGRLSEALGEAMSVAAREGTVCAFLIAAINNLSVVNEAYGFEVADEVVVNMAR
                     RLRQVVRTGDAIGRYSGSKFGLILNSCGEEDLAIAAERFLSVARESVIETERGPVWAL
                     LSIGALILPTHAEDPNTAMARAEEALAEAR"
BASE COUNT         1113 a         2065 c         2087 g         1228 t
ORIGIN      
        1 cggtcgccga tgaccagctc gcgctggccg cggccgatcg ggatcagcgc gtcgatggcc
       61 ttgaggccgg tcgacatcgg ctcgttgacc gacttgcgcg gaatgatgcc gggcgccttc
      121 acgtcgacgc gcttgaaggt cttggacttg atcgggccct tgccgtcgat cgggttgccg
      181 agcgggtcga ccacgcggcc gagcagttcc ttgcccaccg gcacttccac gatggcgccg
      241 gtccgcttga cggtgtcgcc ttccttgatg tcgcggtcgt tgccgaagat cacgatgccg
      301 acattgtcgg tctcaaggtt gagggccatg cccttcatgc cgttggcgaa ttcgaccatt
      361 tcgccggcct gcacgttgtc gaggccgtag acgcgggcga taccgtcacc gacggacagc
      421 acctggccga tctcggtgac ttcggcttcc ttgccgaaat ccttgatctg cttctttaga
      481 attgcggaaa tttcggcggc gcgaatgtcc atcaggcggt ccccttcatg gcgatcttga
      541 gattctgcag tttggtcttg agcgagttgt ccatcatccg agagcccacc ttcacgatga
      601 gaccgccgag aagcgatgaa tcgacccggg ttgagagcgt gacgtcgcgc ccggtcgaag
      661 ccttgagcgc ggccttgagg tccttgagct gaacggccga gagcttgtcg gccgagatga
      721 cttcggcgcc gatctcgccc ttcgagatcg cgaccagcgt gtcgaaggcg tcgatgatgg
      781 cgggaagcgc cggcaggcgg cggttcttga ccaccaggcg gaggaagttg aggctcgtgc
      841 cggtgacctt ggccttggcg cagatggctt cgatggcggc gatctggtcg gcagaggtga
      901 agacgggaga ggtcaccatg cgcttgagat cggcagagcc ttcgatggcg gcgcggaagc
      961 cggcgagctg tgcctgcacg gaatcgaccg atttttcttc cttggcgagg tcgaacaggg
     1021 cgctggcata gcgtcccggg acgccagtaa ctgtctgatc tgtgctagcc acgatccgtc
     1081 tttcattctg cttggcggat agaggccggc gactggcatc gaaccgcttg aattcaggga
     1141 agtgtgcaca caaaacggca cacctgcgga gcgaagcctg cttcgccgcg cgcgttggct
     1201 atcacagtgg aaaaggactc gcaaccggct tgtagtggcg gattgacgcg agacgaaagc
     1261 gaagggcctg cgcagcagtt ttgccgcgca ggcccttcgg aaatcaatat gatcgcggct
     1321 tcagcgcagg gacttgctgc gggcgacatg cccgagctgg tagcgcttca gccccatgtc
     1381 ggcgagatcg gcctcggaga gcgcctcgat ctcagcgcgg gcctgccggt atgccttcat
     1441 ccggcggctc cgcagcagca gctgggccag tccggagaag ccgatcatga gcggacggcc
     1501 tcgcgggcga gccgcggaat gtcgcagcgg ctgatgccga ggtcggcgag ttcgcgggtg
     1561 ctcagcatct ccagttcgga gacggtgcgc gaataacgct tccagacggc gtagcgggat
     1621 ttcaggactt cgaacatggt cattcccctt tggattgaca tcgtgacggt tgctgttgac
     1681 gatcccaatc taggtgcagc gcaacagaaa gagcaatgct atttttgcat atctagactt
     1741 atatcaacta atacagatgt ttataattct gtaatactgt tgaattcgaa tgctgcgctg
     1801 ccgcaaagag cggcatgatg gcagatttta aagcgctttg gagcagagct gcgccgcatt
     1861 tgccccagga ttgtgcacga ctcgcagagc tcgtgagccg ctttgtcgca ggtgcgaaag
     1921 gccggattgg cgccgctgga acgcgaaatg ggcgcaccgg aagcaccggc gcgcccttct
     1981 gaacctcgaa agaggccgac tcggtcctag aacaggccgg cgatctggcc cttgtcgtcg
     2041 agaccgatca cctcggctga cgggaccttg ggcaggcccg gcatggtcat gatgtcgccg
     2101 cagatgacca cgatgaattc cgcgccggcc gagagccgca cctcgcggat cggcaccaca
     2161 tggttggtgg gcgcgccctt gagattggga tcggtcgaga aggaatactg cgtcttggcc
     2221 atgcagattg ggaacttgcc ataaccctgg gcctcgaagg ccttgagctg gtcgcgcacc
     2281 gacttgtcgg ccacgatgtc gtcggcccgg tagatttcct tggcgatggt cctgaccttg
     2341 tcccacagcg agaggctctc gtcgtagatc ggcttgaatt gcgcggcgcc cttgtccgcg
     2401 agctccgcca cgtgcttggc caggccctcg atgcccttgc cgccctcgcc ccagtgggtg
     2461 cacttgaagg cttccacgcc gtggcccttg cagtactcgc gcaccgcctc gatctcggcg
     2521 tcggtgtcgg aaatgaagtg gttgatcgcc accgcgatcg gcacgccgaa cttcttcacg
     2581 ttctcgatgt gccgtccgag gttggcgagg cccttcttca gcgcctcgac attctccttc
     2641 ttgagatcgt ccttggccac gccgccgtgc atcttcagtg cacggacggt cgcgacgatg
     2701 acggttgccg ccggcgtcag tcccgccttg cggcacttga tgtcgaagaa cttctcggcg
     2761 ccgaggtcgg cgccgaaacc ggcctcggtc acgacgtagt cggcgagttt gagcgccgtc
     2821 tgggtcgcca tcaccgagtt gcagccatgc gcgatgttgg cgaagggccc gccatgcacg
     2881 aaggccgggt tgttctccag cgtctgcacc aggttgggca tcagcgcgtc cttgagcagc
     2941 acggtcatgg cgcccggcgc cttgatgtcc ttggccagga cctggccttt gtcgcgcttg
     3001 gcggccacga cgatctggcc catgcggcgc tcgaggtcgg cgatcgaggt cgaaaggcag
     3061 aggatggcca tgatctcgga ggccaccgtg atgtcgaagc ccgcctcgcg cgggaagccg
     3121 ttggccacgc cgcccagcga acagacgatc tcgcgcagcg cccggtcgtt catgtccatg
     3181 acgcggcgcc aggcgacgcg gcgagtgtcg atgccgagtt cgttgcccca gtagatatgg
     3241 ttgtcgatca tcgccgagag caggttgtgc gccgaggtga tggcgtggaa gtcgccggtg
     3301 aagtgcaggt tgatgtcctc catcggcacg atctgggcat agccgccgcc ggcggcgccg
     3361 cctttcatgc cgaagcaggg gccgagcgag ggctcgcgca atgcgatcat ggccttcttg
     3421 ccgatgcggt tgaggccatc gccgaggccc acggtggtgg tggtcttgcc ttcgcccgcc
     3481 ggcgtgggat tgatggcggt caccaggatg agcttgccgt ccttcttgga cttcaggctg
     3541 tcgatgtagt cgagcgacac cttggccttg gtgtggccgt agggaatgag gtccttctcc
     3601 ggaatgccga gaccccgtcc gatttccatg atcggcttca tcttggcttc gcgggcgatt
     3661 tcgatatcgc tcttcaccgt ggacatgtgg ggcggctcct cattggtgtt tgaattgggg
     3721 cggggcgtaa catagtctcg acaggctgcg cagcgttcca accgacatga tcacgccgtt
     3781 ttcgacgccc aatcacaacc aattcagacc aataacagcc aatcttgcgc cgaaagggca
     3841 ggaaaaggca atgaccaatc cataaaccca gtctttgaca ggcccattca tgcgtgcttt
     3901 cgaccgaccc ggacgctcca ccgcctattc gacgtccggc atggccgcga cttccgcccc
     3961 gatggccacg ctggccgcgg tggaatgcct caaggcagga ggcaatgcgg tggatgccgc
     4021 tgtcacggcc tcggcggtgc tctgcgtcgt cgagccggcc atgaccggta tcggcgggga
     4081 ctgctttgcc ctgatcggca cgccggatgg caaggtgcag ggcctgaacg cctccgggcg
     4141 gtcagcccgg gccgccgatg ccgactggct cagcgcctcg ggcctcaccc gcatcgcccc
     4201 ccgcagcatt catgccatca ccgttccagg cgccatcgac ggctgggacc agctgctcag
     4261 ggcctacggc accaggtcgc tcgccgattg cctggtgccc gccatcggta tggccgaggc
     4321 cggaactccc gtcaccccgc gcgtcgccct ggactggccc gaagacgcgc cggatctcct
     4381 ggccgacgcc gggggtcagc agcactacct gaagaatgga ctggcgccgc aggagggcga
     4441 cgtcatcgcc tatccggccc tggcccggac attgcggctc atcgcccggg agggccgtga
     4501 cgccttctat gcggggccta tcgccgccga catcgtgggc catgtccgcg ccatgggatc
     4561 gctgctgacg cttgcggatt tctccagcca tacctcgacc tgggtggagc ccatttccac
     4621 cggcttcgcc gggcatgaag tgcttgaaat tccacccagc ggccagggtc tgaccgccct
     4681 catcgcactc aatgtcgtgt cccatttcgg cctcgcgcgg cacgagcccg attcgcccga
     4741 tcgcatgcac atcgaaatcg aggcgatgaa actggcctgg gaactgcgca accgccatat
     4801 cgccgatccg gacttcgccg aagtgcccgt ggcagagctt ctcagccccg gtatggccgc
     4861 aaggctggcc ggcatgatcg acatgaagca ggcgctcgat atccggaccg ccatgcgcca
     4921 gtcggacacc atctatctca gtgtcgtcga ccgcaaccgg ctggccgtct ccttcatcaa
     4981 ctcgatctat tacggcttcg gatcggccat cgtcacgccg gcaaccggca tcagcctgca
     5041 gaaccgcggc gcctgcttcg tcgccgatcc cggccatccc aactgcatcg gcccggcgaa
     5101 gcggccgcta cacaccatca ttccggccat gtcccgcaag gacggacgcg tcgacatgag
     5161 cttcggggtg atgggcggcg actaccagcc gatgggtcac ctctgcgttg ccgtcaaccg
     5221 ctatatctat ggcatggatc ctcaggcggc catcgacatg ccgcgctact tccccaaggg
     5281 tggcgaggtg ctggtggaaa gcggtgtgac cgaagctgcc caggccggac tgcgcgaccg
     5341 cggtcatgcc ctggttccag cgcccgaacc gctgggcggc ggccaggcca tcgtgatcga
     5401 ccatcgccgc ggaattctca tcggcggatc ggacccgcgc aaggatggcc tggcgctcgg
     5461 catttgaata acgacgttaa gaaaagattt tccctgctca actaaggtca gtccgggtca
     5521 ggcccccgcc ccggattgcc ggagcgggag tccggcacca gttgcggagg ccgcgtgagc
     5581 agcgatagtt cgggaaatca ccacaggtcc ggcactgcca aggccgcgga tccgctcggc
     5641 agcgcactgc gcgccatgga agccgcgggc gtcgccacct acaactgggt gatcgacacc
     5701 gacgagatcg tctggtcatc caatgccgcc gatgtcatgg gctgcgatcc cggagagatt
     5761 gccaccggcc gcgcctttgc gtcctatctc gaccccgaca atttcaccag ccgctacgat
     5821 gcggtcatgc gcaccaccgt catcgacgac ggcgacggcg tacccttcca gatcgaatac
     5881 aagttccgcc ccgaaggccg ccttggccgc gccagcctct ggctggagga tcacggcagc
     5941 tggttcggcg gcaaggatgg ccgtcccgtc gaagtcttcg gcacggtgcg ccgcatcgat
     6001 gcgaggcacc agcgcgacca gcacctgagc ttcctcggca actgcgatcc gctcaccgga
     6061 atgatgaacc gtggccgcct gtccgaggcc cttggcgaag ccatgtcggt cgccgcccgc
     6121 gagggcacgg tctgcgcctt cctgattgcc gccatcaaca atctttccgt ggtcaacgag
     6181 gcctatggct ttgaagtggc cgacgaggtc gtggtcaaca tggcgcgccg cctgcggcag
     6241 gtggtgcgta ccggcgatgc catcggacgc tactcgggca gcaagttcgg cctgatcctc
     6301 aacagttgcg gtgaggagga tcttgccatc gcggcggagc gcttcctcag cgtggcgcgc
     6361 gagagcgtga tcgaaaccga acgcggtccg gtctgggcgc tgctgtcgat cggcgcactg
     6421 atcctgccca cccatgccga agatccgaac acggcaatgg cccgggcaga ggaagcgctg
     6481 gccgaggcga ggc
//
LOCUS       DBNGVR010000073         2983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2382653, whole genome shotgun sequence.
ACCESSION   DBNGVR010000073 DBNGVR010000000
VERSION     DBNGVR010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2382653"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="AC16A0_01605"
     CDS             <1..437
                     /locus_tag="AC16A0_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3104053.1"
                     /translation="GAGVGYGYGGDGDPMKLEGFVDMTRCLLIDQDGASQRNLLAMLD
                     ALGIDAARASAADEALKFCNDNAPEVVMVAAAAPGVQPHELMKRLKRSGNGRPPVVFL
                     YAETPDTDVIGQSIIEGAADVLMTPLDRDILEFKLRQAGIIA"
     gene            491..1705
                     /locus_tag="AC16A0_01610"
     CDS             491..1705
                     /locus_tag="AC16A0_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3104054.1"
                     /translation="MAHYSIWILLVCVGLLCIGHGLHGSLVAVIAQSSDFGPDVTGLV
                     MSGYAAGLLVSSFITPRLVQSVGHVRAFAGLASIVSTAVLLMPLWVNAPFWLAMRFVS
                     GLCTSGLFIVCESWLNGASSNRNRGQMLSVYMIVTYGALGAGQLLLNVTDTSGFARFI
                     IVSALLSLSLVPLILLPAEAPSIEGARPVSIEEVWRASPLAVVGAFACGLAQSGFFAM
                     GAVFGLTQGLALVYVSIMMALPPIGVIFSQYPIGWISDRIDRRTVIMMLSAVSAVIAT
                     LTMLTDGFTPVLFIGMVTIFGTLSLPLYSLVIAHANDHLVKEQILGASAKLVLLYGLG
                     SILGPLTVGQFMQSMGGGGFLAFNALDYASLAGFAFWRRLKRPEDIRGKAGEAMKVSP
                     VTTPSGASALQD"
     gene            1819..>2983
                     /locus_tag="AC16A0_01615"
     CDS             1819..>2983
                     /locus_tag="AC16A0_01615"
                     /inference="COORDINATES: protein motif:HMM:NF021603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HIR3104055.1"
                     /translation="MKDEPGFGLDTSIFDDVIENGPVAQRLVLARQLAAFLARDGVAK
                     AEHDQVVPVVLKLAVDPVSDVRRCLATALADAPEIHADILFSIVSDDDEIALPFLAAT
                     PALNHWHMLAVLRVGDAARQSTVIMRADVSDEAVAYAVKSLPLNVCLLLFDNPEIQLD
                     DQQYHALYARFGHAGDMTEKLLACPDLPLDIRIMQAKRASNRMHQLMAERGWLPANDA
                     VELVHDAEENAVLRILIEATDVELAKLVAFLVSKSMLTTSIIVRAACLGEMHVVERAL
                     AHLADVPLQRARGLMRGKGLTGFRGLHAKSGLPQSCYWILQAACDVSAEEAEEGVTLN
                     AEDFGRRVIEALMTRYESMPLKERTRHLEYVGRFAADRARVIAKRLKADLVRAA"
BASE COUNT          500 a          920 c          926 g          637 t
ORIGIN      
        1 ggggcgcagg agtcggatac ggatacggcg gggatggcga tccgatgaag ctggaaggat
       61 ttgtagacat gacaagatgt ctgctgattg atcaagacgg agcgtcgcag cgcaatctcc
      121 tggccatgct cgatgccctc gggatcgatg ccgcccgggc ttctgccgct gacgaagcat
      181 tgaagttctg caatgacaat gctcccgaag tcgtcatggt cgccgcagct gcccccgggg
      241 tgcagcctca tgaactcatg aagcgcctca agcgcagcgg gaatgggcgc ccgccggtgg
      301 tgttcctcta tgccgagacg ccggacaccg atgtcatcgg tcagtcgatc atcgaaggtg
      361 cagcagatgt actgatgacg cctctcgatc gcgatattct cgaattcaag ctgcgccagg
      421 ccggaatcat cgcctgatac tgcgcgccag tcacttggcc ttgcccccga atctggcgca
      481 aaggtgacgg atggcgcact attccatctg gatactcctc gtctgtgtcg gtctgctctg
      541 catcggccat ggcctgcacg ggtcattggt tgcggtgatc gcgcagtcct ccgatttcgg
      601 acctgacgtg accggtcttg tgatgtcggg ttatgcggcc ggcctgctgg tgagttcctt
      661 catcacgccc cgcctggtgc agagcgtggg gcatgtccgc gcctttgccg gactcgcttc
      721 catcgtctcg acggcagtgc tgctcatgcc gctatgggtc aatgcgccgt tctggctggc
      781 catgcgcttt gtctcgggac tgtgcacgtc tggcctcttc atcgtctgcg aaagctggct
      841 caatggcgcg tcgtcgaacc gcaaccgcgg ccagatgttg tccgtgtaca tgatcgtcac
      901 ctatggcgcg cttggtgcgg gccaactgct gctcaacgtg acggatacgt cgggcttcgc
      961 gcgcttcatt atcgtttcgg cactgctgtc gctgtcgttg gtgccgctca tccttttgcc
     1021 tgcagaggcg ccgagcatcg agggagcgag gccggtgagc atcgaggagg tctggcgggc
     1081 gtcgcccctc gccgtcgttg gcgctttcgc ctgcggactg gcgcagagtg gcttttttgc
     1141 catgggcgct gtcttcgggc tcacgcaggg cctggcgctt gtctatgtct cgatcatgat
     1201 ggcattgcct ccgatcgggg tgatcttctc gcagtatcca atcggatgga tctcggaccg
     1261 tatcgaccgc cgcaccgtga tcatgatgct gagtgccgtc agcgcggtca tcgcgaccct
     1321 cactatgctg accgacggtt tcacgccggt gctcttcatc ggcatggtca cgatctttgg
     1381 cacgctgtcg ctgccgctct attcattggt gatcgctcat gccaatgacc atctggtgaa
     1441 ggagcagatc ctgggcgcct cggcgaagct ggtgctgctc tatgggcttg gctcgattct
     1501 cggacctctg accgtgggcc agttcatgca gtcgatgggc gggggcggct tcctggcctt
     1561 caatgccctg gactatgcct cacttgccgg ctttgccttc tggcggcggt tgaaacgtcc
     1621 ggaagacatc cgcggaaaag cgggggaggc catgaaggtg agcccggtca cgaccccatc
     1681 gggagcatcg gccctgcagg attgagccaa ctcccgccgc cacaggtgtt agcaactccc
     1741 taacagccgt ggtgaaccaa atcttaaatc ttgagtggca cggttcccct tcatgatgcc
     1801 gaaggtggga cgattgcctt gaaagacgaa cccggttttg gactcgatac cagcatcttc
     1861 gatgacgtga tcgaaaatgg cccggttgcc cagcgcctgg ttctggcgcg gcagctcgcc
     1921 gccttccttg cacgagacgg tgtcgccaag gccgaacacg atcaggtggt acccgtcgtg
     1981 ctgaagctgg ccgtggaccc cgtgtcggac gtccgccgtt gccttgccac ggcgctggcc
     2041 gacgcgcccg aaattcatgc cgacatcctg ttctccatcg tttctgacga cgacgagatc
     2101 gcattgccct tccttgcagc aacgccggcg ctaaatcact ggcacatgct ggccgtgttg
     2161 cgggtgggtg acgcggcccg ccagtcgacc gtcatcatgc gtgcggacgt cagtgacgag
     2221 gcagtggcct acgccgtgaa gtcgctgccg ctcaacgtct gccttctgct cttcgacaat
     2281 cccgagatcc agttggatga ccagcagtat cacgcgctct acgcgcgctt cggccatgcc
     2341 ggcgacatga ccgagaagct gctcgcttgc ccggacctgc cgctcgatat ccgcatcatg
     2401 caggccaagc gcgcctcgaa ccggatgcat cagctgatgg cggaacgcgg ctggcttccg
     2461 gccaatgacg ccgtcgagct cgtccacgac gccgaagaga atgccgtgtt gcgcattctg
     2521 atcgaagcca ccgacgtcga actggcaaag ctggtcgcct tccttgtgag caagtccatg
     2581 ctcacgacat ccatcatcgt gcgtgccgcc tgccttggcg agatgcatgt ggtcgagcgt
     2641 gccctggcac atctggcgga tgtgccgctg cagcgggcgc gcggcctgat gcgcggcaag
     2701 ggattgaccg gtttccgcgg actgcatgca aagtcaggcc tgccgcagag ctgctactgg
     2761 attctgcagg cagcctgcga cgtctctgcg gaagaagccg aagagggcgt aaccctcaat
     2821 gcggaggatt ttggccgccg ggtgatcgag gcgctcatga cccgctacga atccatgccg
     2881 ctcaaggaac gcacccgtca tctcgaatat gtcggccgtt ttgccgccga ccgggcccgc
     2941 gtgatcgcaa agcggctcaa agcagatctg gtacgcgccg cct
//
LOCUS       DBNGVR010000074         4830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1007847, whole genome shotgun sequence.
ACCESSION   DBNGVR010000074 DBNGVR010000000
VERSION     DBNGVR010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1007847"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16A0_01620"
     CDS             <1..417
                     /locus_tag="AC16A0_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007632740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR3104056.1"
                     /translation="GGIAGYLLGYFAYEAVAKPVLAFYGKLDSFEALRSCAGEDTLMM
                     LLVTSGLSHLPPIKVVTILAGVALISLVFFIVSCVIARGARFFALAWALRRFGEPIRH
                     FIERRLGLIAALAAAALIALYFILKYLAGTGSLTAC"
     gene            411..890
                     /locus_tag="AC16A0_01625"
     CDS             411..890
                     /locus_tag="AC16A0_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR3104057.1"
                     /translation="MLTSLSTRHALLLLLVVSIATIAGAWIFEGAGYLPCELCLMQRW
                     AYYAAIPLTAVLLVAAPLQRAGLYLLALLWIGSMIFGIFHSGVEWKWWAGPTACTGSG
                     ALSMGLPDLSSQVVMCDQPAIRIFGLSLAGWNAVISLGLALVALAGARSHGSSSVSQ"
     gene            complement(863..1429)
                     /locus_tag="AC16A0_01630"
     CDS             complement(863..1429)
                     /locus_tag="AC16A0_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3104058.1"
                     /translation="MTVHYGPIAPEACPALVLNADFRPLSYYPLSLWSWQDTIKAVFL
                     DRVNIVSEYDRVVRSSSFEFRLPSVVSLKTYVKPSRTPAFTRFNVFLRDRFTCQYCGN
                     HHDLTFDHVTPRSKGGQTTWENVVTACSPCNLLKGGYFPHEIHMYPAQKPARPSVAQL
                     HRNGRLFPPNYLHDSWQDYLYWDTELDP"
     gene            complement(1505..2140)
                     /locus_tag="AC16A0_01635"
     CDS             complement(1505..2140)
                     /locus_tag="AC16A0_01635"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR3104059.1"
                     /translation="MQSYSSQDDLAAAVAELSRRDGRLGLLAARHGVPPLRRMEPGLA
                     SLLRIVTDQLISLKAAAAIWQRLEAGLPMGDANSLAAASEDRLRGFGLSGGKARSFRA
                     IAQAVVAGALDVQDLGLLDDAAVLKRLQSIHGIGPWTAQIYLLMALNRSDGWPAEDLA
                     LQLAVQDLYGLPGRPRPAAMRAMAEAWRPWRGAAAHLLWRHYRDLRGIAPD"
     gene            2184..2978
                     /gene="gluQRS"
                     /locus_tag="AC16A0_01640"
     CDS             2184..2978
                     /gene="gluQRS"
                     /locus_tag="AC16A0_01640"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR3104060.1"
                     /translation="MTTAVFRFAPSPNGELHLGHAYSALLNDEMARSLGGRLLLRIED
                     TDLTRCKPSFTAGIFRDLAWLGLAWEEPVRIQSEHFGDYDANLLKLWDAEAIYPCFCS
                     RRQAAAEALPQRDPDGQAHYGRRCRALTRHEAASRMAAGAQFGWRLDMVRAGDAAAAA
                     WGDVVIAKTKVGSYYHIAVVTDDALQGVTQVVRGLDMEPATPIHMLLQRLLGLPAPAY
                     HHHRLILDADGRKLSKSSNSTALASLREQGVTAEQIRSQLGFAPAS"
     gene            complement(2975..3160)
                     /locus_tag="AC16A0_01645"
     CDS             complement(2975..3160)
                     /locus_tag="AC16A0_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HIR3104061.1"
                     /translation="MTSMPEHKTTLTPEARRALAEAEARRKTRTEQPLPKEVNGRAGP
                     EPTRYGDWETKGIASDF"
     gene            3206..4123
                     /gene="htpX"
                     /locus_tag="AC16A0_01650"
     CDS             3206..4123
                     /gene="htpX"
                     /locus_tag="AC16A0_01650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIR3104062.1"
                     /translation="MNTFRTGLLLAALTGLFMAVGYLIGREQGMIIAFLVAAATNLFA
                     YWNSDKMVLRMYGARQVDRNTAPEYYGMVEELAHRAGLPMPKVFIMDNPQPNAFATGR
                     NPQNAAVAATTGLLKLLTPDEVAGVMAHELAHIKHRDTLVMTMTATIAGAISMLANFG
                     LMFGGGNNRNNPLGAIGTILMVILAPLGAMLVQMAISRTREYGADRGGADISGNPHAL
                     ASALAKISNAAHRIENRPAEANPATAHMFIINPLSGARMDNLFSTHPNPKNRIDALMA
                     MARETGGDRSSADSGPWSGTPSERQGPWG"
     gene            4128..>4830
                     /locus_tag="AC16A0_01655"
     CDS             4128..>4830
                     /locus_tag="AC16A0_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3104063.1"
                     /translation="MQDTQQGLDVRRLAVLCLTEILEGQRLSDEVIERRPDVAALAPR
                     DRAFLRAIVMATLRHAGEIDAVLAEFLAKPLPRKAGPARQILETAAAQLLFMEVAPHA
                     VVDLSVRLAKADANATHFSGVINGVLRNMARRGHEVLARVGGARRNVPDWCWQRWVSA
                     YGDADAAAIAESRLEEPALDLTPRADAALWAERLGGVLLPTGSIRLPMNHSAIEELPG
                     YSEGAWWVQDAAAAIP"
BASE COUNT          765 a         1594 c         1608 g          863 t
ORIGIN      
        1 ggcggcatcg ccggctacct tctgggttac ttcgcctacg aggccgtggc caagccggtt
       61 cttgccttct acggcaagct cgacagtttc gaggccctgc gcagttgtgc cggcgaagac
      121 acgctgatga tgttgctggt cacctcgggc ctgtcgcatc tgccgccgat caaggtggtc
      181 accatcctgg ccggcgtggc cctgatcagc ctcgtcttct tcatcgtctc ctgcgtgatc
      241 gcgcgcggcg cccggttctt cgcgctggcc tgggcactgc gccgtttcgg cgagccgatc
      301 cgccacttca tcgagcgccg actcgggctg atcgccgcgc tggcggcggc tgccttgatc
      361 gcgctgtatt tcatcctcaa gtacctggcc ggaaccggat cgctcactgc atgctgacct
      421 cgctctcgac ccgccacgcc ctgctgctgc tcctcgtggt atccatcgcc accatcgcgg
      481 gcgcttggat cttcgaaggc gcgggctatc tcccctgcga actctgcctc atgcaacgct
      541 gggcctatta cgcggccatt cccctcaccg cggtgctgct cgtcgcagct ccgttgcaac
      601 gcgccggact ctacctgctg gcattgctgt ggatcggcag catgatcttc ggcatcttcc
      661 attccggcgt ggagtggaag tggtgggcgg ggcccaccgc ctgcacggga agcggcgccc
      721 tgtcgatggg cctgcccgac ctgtcgagtc aggtcgtgat gtgcgaccag cccgccatcc
      781 ggattttcgg cctctccctc gcggggtgga atgcggtgat ctctctgggc ctcgcgcttg
      841 tcgcgctggc cggtgcgcga tctcacggat cgagttcggt atcccagtag aggtagtcct
      901 gccagctgtc gtgcaggtag ttcggcggga agaggcggcc attgcgatgc agctgcgcaa
      961 cgctgggccg cgccggcttc tgcgcgggat acatatggat ctcgtgcggg aagtagccgc
     1021 ccttgagaag attgcagggc gagcaggcgg tgacgacatt ctcccaggtg gtctgcccgc
     1081 ccttcgagcg cggcgtcacg tgatcgaagg tgagatcatg atgattgccg caatactggc
     1141 aggtgaagcg gtcgcgcagg aaaacgttga accgcgtgaa ggccggcgtg cgcgacggct
     1201 taacataggt cttgagcgaa accacgctgg gcaggcggaa ttcgaaactg gaggagcgca
     1261 ccacgcggtc atactcagag acgatgttca cgcggtcgag gaagacggcc ttgatcgtgt
     1321 cctgccagct ccacagcgac aggggataat aactcaacgg gcggaagtcc gcgttgagaa
     1381 ccagggcagg acaggcttcc ggcgcgatcg gcccgtaatg cacagtcacc tcgtaggttt
     1441 gttcacctga atcaatacta tatctagtgt gacgcctctg tgaagtgcct ccatgccgcg
     1501 ggggtcagtc cggggcaatt ccgcgcaggt cgcgataatg gcgccacagc aggtgggcgg
     1561 cagctccccg ccagggccgc caggcctccg ccatcgcgcg catggcggca ggtctgggcc
     1621 ggcccgggag gccgtaaagg tcctggactg ccagttgcag ggcaaggtcc tccgccggcc
     1681 agccgtcgct ccggttcagc gccatgagca gatagatctg tgccgtccag ggtccgatgc
     1741 cgtgaatgga ctggaggcgc ttcagcacgg cagcgtcgtc aagcagccca aggtcctgga
     1801 cgtcgagcgc gcctgcaacg acggcttgcg cgatggcccg gaagctgcgc gccttgccgc
     1861 ctgacaggcc gaagccgcgc aggcggtctt cgctggccgc ggcaaggctg ttggcatcgc
     1921 ccatgggcaa gccggcctcc agcctctgcc agatggcggc ggcggccttg agcgagatca
     1981 gctggtcggt gacgatgcgc aagaggctcg caagacccgg ctccatgcgc cgcagcggcg
     2041 gcacgccatg gcgggccgcg agcaggccca gtcgcccgtc acgccgcgac agttcggcga
     2101 cggcagcggc aaggtcatcc tggctcgagt agctttgcat cgccgcattt gacctggacc
     2161 gccggctggc gcaagcaggc gcgatgacga cagcggtgtt tcgtttcgct cccagcccca
     2221 atggcgagct gcacctggga catgcctatt cggccttgct caatgacgag atggccaggt
     2281 ccctgggcgg ccggctcctg ctgcgcatcg aggacaccga cctcacccgc tgcaagccca
     2341 gcttcacggc cgggatattc cgggacctcg cctggctggg gcttgcctgg gaggaaccgg
     2401 tccggatcca gtcggagcat ttcggcgact acgatgccaa tctcctgaag ctgtgggacg
     2461 cggaagccat ctatccctgt ttctgctcgc gccggcaggc tgcggccgaa gcactgccgc
     2521 agcgcgatcc cgatggccag gcccattatg gccggcgctg ccgcgccttg acgcggcacg
     2581 aggccgcgtc tcgcatggcg gcgggcgcgc agttcggctg gcggctcgac atggtccgcg
     2641 ccggcgacgc cgcagcggcg gcctggggcg acgtggtgat cgcaaagacg aaagtgggct
     2701 cctactatca cattgcggtg gtgaccgatg acgcgctgca aggcgtcacg caggtcgtgc
     2761 gtgggctgga catggagccg gcgacgccca tccacatgct cctccagcgc ctcctcggcc
     2821 tgccggcccc ggcctatcac caccacaggc tgatcctcga cgccgatggc cggaagctct
     2881 cgaagtcaag caacagtacg gcgctggcgt ctctgcggga gcaaggcgtt acggcggagc
     2941 agatccgcag ccagcttggt ttcgctccgg caagctagaa gtcgctggcg atgcccttcg
     3001 tctcccagtc gccatagcgc gttggttcag ggccggcgcg gccgttcact tccttcggca
     3061 ggggctgctc ggtgcgggtc ttgcgccggg cttccgcttc cgccagggcg cgtcgggctt
     3121 cgggggtcag agtggtcttg tgctcgggca tcgatgtcat catatgatgg cggagaaccc
     3181 gtttcatcaa gtagtttcgc tgaccatgaa tacctttcgg accggactgc tgctggcggc
     3241 cctgaccggg cttttcatgg cggtgggcta cctgatcggc cgcgagcagg gcatgatcat
     3301 cgccttcctg gtggcggcgg cgacaaatct cttcgcctac tggaactccg acaagatggt
     3361 gctgcgcatg tatggggccc gccaggtgga ccgcaatacg gcgccggagt actacggcat
     3421 ggtcgaggag ctggcacatc gcgccgggct gcccatgccc aaggtcttca tcatggacaa
     3481 tccgcagccc aacgccttcg cgaccgggcg caacccgcag aacgcggcgg tggccgccac
     3541 caccggcctg ctcaagctgc tgacgcccga cgaggtcgcc ggcgtcatgg cccatgagct
     3601 ggcccacatc aagcatcgcg acacgctggt catgaccatg acggccacca tcgccggcgc
     3661 catttcgatg ctggccaatt tcggcctcat gttcggcggc ggcaacaacc gcaacaatcc
     3721 gctgggcgcg atcggcacca tcctgatggt gatccttgcg cccttgggcg ccatgctggt
     3781 gcagatggcg atcagccgca cgcgcgaata tggcgccgac cgcggcggcg cggacatctc
     3841 cggcaatccg catgcgctcg cctccgcact tgccaagatc tccaacgccg cccatcgcat
     3901 cgagaaccgc ccggcggaag ccaatccggc cacggcgcac atgttcatca tcaatccgct
     3961 ttcgggcgcg cgcatggaca atctcttttc cacccatccc aatccgaaga accggatcga
     4021 tgcgttgatg gccatggccc gggaaaccgg cggcgaccgg agttcagcgg attcgggtcc
     4081 gtggagcggc acgccgtcgg agcgccaggg tccctggggc tgagattttg caggacacgc
     4141 agcagggtct cgatgtccgc cgccttgcgg ttctctgcct caccgaaatt ctcgagggcc
     4201 agcgcctttc cgacgaggtg atcgagcgcc gcccggatgt cgcagcactt gcgccgcgcg
     4261 atcgcgcctt cctgcgggcc attgtgatgg cgacacttcg ccatgctggc gagatcgacg
     4321 cagtgctggc ggagttcctc gcgaagcccc tgccccgcaa ggcaggaccg gcccggcaga
     4381 tcctggagac ggccgccgca cagcttctct tcatggaggt ggcgccgcat gcggtggttg
     4441 atctctccgt gcgcctggcc aaggccgatg ccaacgccac ccacttcagc ggcgtgatca
     4501 acggcgttct gcgcaacatg gcacggcgcg gacatgaggt gctggcaagg gttggcggcg
     4561 cgcggcgcaa cgtgcctgac tggtgctggc agcgctgggt gagcgcctat ggcgatgccg
     4621 acgcggcggc gattgcggag tcgcggctgg aggagccggc acttgatctc acgccccggg
     4681 ccgatgcagc gctctgggcc gagcggctgg gcggagtcct tctgccgacc ggcagcatcc
     4741 gtcttccgat gaaccactct gccattgaag agctgccggg gtacagcgaa ggggcctggt
     4801 gggtgcagga tgctgcggcc gccataccgg
//
LOCUS       DBNGVR010000075         2345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1597639, whole genome shotgun sequence.
ACCESSION   DBNGVR010000075 DBNGVR010000000
VERSION     DBNGVR010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1597639"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..445
                     /locus_tag="AC16A0_01660"
     CDS             131..445
                     /locus_tag="AC16A0_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3104064.1"
                     /translation="MSTIKVIDQHGVSHILDAVEGWRVMEIIREHGFDVAGLCGGACA
                     CATCHVEVDGEWLSKLHPANDDEEAMLDQVPLLTDGSRLSCQIIFTPELAGLQVRLVA
                     NG"
     gene            455..1477
                     /locus_tag="AC16A0_01665"
     CDS             455..1477
                     /locus_tag="AC16A0_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104065.1"
                     /translation="MRYFPIFYDLRDRLVVVVGGGEEALRKIRLLLKTEARIAVIAED
                     LHPELAAEPRVTWLAKSYAKELLSGAALVYSADPALNETVAQDAALMGLPVNAVDNPG
                     ISTFIVPSIVDRDPLIVAIGTEGTAPILGQGLRARIDAMLPPALGPLARAASALRERV
                     AATIPAGNRRRSFWTRFFFGDVRDAFVAGETVNYLAGVEGLFTNEAAPPVGRVSFIGV
                     SSDDPELLTIKAQRKLQEADLIVHDTGIDAGILEVARRDAVRRPVAGRLFDGTDQTLV
                     REAKAGKHVVRLSCGEPSLEETVAVAAEGIAFETVPGIPPARVTKAEPFPSHDTLRDA
                     ILRAAS"
     gene            1474..1815
                     /locus_tag="AC16A0_01670"
     CDS             1474..1815
                     /locus_tag="AC16A0_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HIR3104066.1"
                     /translation="MTTTEKGQIVTANRLRDGIAVFLTRSGEWSEAIDDAVLALEPEA
                     ANALEQRARLAEKACLVTGSYLVDAERRAGQVRAAHIRERMRALGPTVRPDLGKQAEG
                     KANSFAAPEGV"
     gene            1819..>2345
                     /locus_tag="AC16A0_01675"
     CDS             1819..>2345
                     /locus_tag="AC16A0_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3104067.1"
                     /translation="MYRYDEFDHQFVRERTAQFRDQAERRLHGELTEDEFRPLRLMNG
                     LYLQLHAYMLRVAIPYGTLSGRQMRQLAMIADTYDKGYGHFTTRQNIQYNWIKLEETA
                     DILGHLADVEMHAIQTSGNCIRNVTTDHWAGAAADEIADPRPVGELLRQWSSLHPEFS
                     YLPRKFKIAVTGAPH"
BASE COUNT          450 a          721 c          732 g          442 t
ORIGIN      
        1 cagccaatga acggtgactt gaacgggtcc agttacagca gtttcgcctt aataatcaaa
       61 cgaaatctga agactgggta actcgcgtac gatagaccga acgtttgctg gagcgaacac
      121 ggataggacg atgagcacga tcaaggtcat tgaccagcat ggtgtaagcc acattctcga
      181 cgccgtggaa ggctggcggg tcatggaaat catccgtgaa cacgggttcg atgttgcagg
      241 cctctgcggc ggtgcctgtg cctgcgccac ctgtcatgtc gaggtggatg gagaatggtt
      301 gtccaagctt caccccgcca atgacgacga ggaggccatg ctggaccagg ttccccttct
      361 gaccgacggt tcgcggctgt catgccagat cattttcacg cctgagctcg ccggcctgca
      421 agtcaggctc gtggccaacg ggtgaggaca caggatgcgt tactttccga ttttctatga
      481 cctgagggac cggctcgtgg tggtcgtcgg cggcggcgaa gaagcactcc gcaagatccg
      541 gctgttgctc aagacggaag cacggatcgc cgtgattgcg gaggatcttc atccggagct
      601 ggcagcagag ccgcgcgtca catggctggc caagtcctat gcgaaggaac tgctttccgg
      661 ggcggcattg gtgtattcgg ccgatccggc cttgaacgag acagtggcgc aggatgcggc
      721 tttgatgggc ctgccggtca atgccgtcga caaccccggc atctcgacat tcatcgtgcc
      781 ctccatcgtg gatcgcgacc cattgatcgt ggccatcggc accgaaggga ccgctccgat
      841 cctgggacaa ggtctacggg cacgcatcga cgcgatgctt ccgccggcgc ttggaccgct
      901 ggcccgcgcc gcctcggccc tgcgcgaaag ggttgccgcc acaataccgg ccggcaatcg
      961 ccgccgctcc ttctggacgc gtttcttttt cggcgatgtc cgggatgcct ttgtcgccgg
     1021 cgaaacagtc aactacctcg ctggggtgga agggctcttc accaatgaag ccgctccgcc
     1081 ggtcggccgc gtctccttta ttggtgtttc atcggatgac ccggagcttc tcaccatcaa
     1141 ggctcagcgc aagctgcaag aagccgatct catcgtgcac gacactggca ttgatgcggg
     1201 aattctggag gttgcccggc gcgacgctgt ccggcgtccc gttgcaggcc ggctcttcga
     1261 tggaaccgat cagaccctcg tccgggaagc caaggccggc aagcacgttg tgagattgtc
     1321 ctgcggcgaa ccgtctttgg aggaaaccgt ggcggtggct gcggaaggca tcgctttcga
     1381 gaccgtcccg gggatacccc cggctcgggt aacgaaagct gaaccctttc cgtcccatga
     1441 cacgctccgc gacgccattc tgagagctgc atcatgacca cgacagagaa aggccagatc
     1501 gtcaccgcga accgtctgcg cgacggcatc gcagtcttcc tgactcgctc cggcgagtgg
     1561 agcgaggcga tcgacgatgc ggttctggcg ctggaaccgg aagctgcaaa tgctctggag
     1621 caacgcgcca ggctggcgga aaaagcctgc ctggtgaccg gctcctacct cgtggatgcg
     1681 gagcggcgtg ccggccaggt tcgggctgcc cacatccgcg agcgcatgcg ggccctgggg
     1741 cccacggtcc gccccgatct gggcaagcag gcggaaggca aggcaaacag ctttgccgcg
     1801 cctgagggag tgtgagccat gtaccgctat gacgagtttg accatcagtt cgtgcgcgag
     1861 cgcacggccc agtttcgcga ccaggctgag cggcgcttgc acggcgaact gaccgaggat
     1921 gagttccggc cgctgcgcct gatgaacggc ctctacttgc agcttcacgc ctacatgctg
     1981 cgggtggcga taccctatgg cacgctgtca ggccgtcaga tgcgccaact cgccatgatc
     2041 gccgacacgt atgacaaggg gtacggccac ttcaccacgc gccagaacat ccagtacaac
     2101 tggatcaagc tggaagagac ggccgatatt cttggccatc ttgccgatgt cgagatgcat
     2161 gccatccaga cctcgggcaa ctgcatccgc aatgtgacga cggatcactg ggccggtgcc
     2221 gcagcggacg agatcgccga tccccggccc gtcggcgaat tgttgcgcca atggtcctcg
     2281 cttcatcccg aattctccta cctgccgcgc aagttcaaga tcgcggtgac aggtgccccg
     2341 catga
//
LOCUS       DBNGVR010000076         4114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3955858, whole genome shotgun sequence.
ACCESSION   DBNGVR010000076 DBNGVR010000000
VERSION     DBNGVR010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3955858"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="AC16A0_01680"
     CDS             complement(<1..911)
                     /locus_tag="AC16A0_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104068.1"
                     /translation="MNNSQQWPESVSETEDESVHPDPQWIFERETVEGLRRPAPDSEP
                     VRRPEMPAKTEARRLPMAPRNFEFGAKLEARAAARAMEAPSANPSLLRKTYVIAALAA
                     IVAGGGAGFIGSQAGPMISAMTAPEAAPVSGQPDQAMADLPKLVTATAAPAAAAETVI
                     TKKTIPIATLNVSDAAGEANSQIPLALSAEPAFEGQDLILKISGVPESAYLTAGTKAA
                     DNTWELPHAKLSGVSLVTTSPQEGSFDLTVAGFAPSTGELVTPIKEFKVQIGDGTPTI
                     QPASAPPDTAILKVSTSPSPQAVILPDA"
     gene            1127..2026
                     /locus_tag="AC16A0_01685"
     CDS             1127..2026
                     /locus_tag="AC16A0_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR3104069.1"
                     /translation="MARIRGRKLRLVMPEGGDERIRMAAQRLVSEDLARPIVFSEPDI
                     PEADGRHIALIRARREKLTDAMAARLLKKPLYRAGAMVAAGEAEAMLAGAANPTARVI
                     EAALMTIGLAPGISIPSSFFLMQWPDRRLIFADCAVNVQPTAEELAAIARASAESARR
                     VLGETPRVALLSFSTKGSGNHPDADKVVAALALARAQAPGLLIDGELQGDAALSAAVA
                     ARKMAEPGPVAGQANVLVFPDLDAGNIAYKLTQYLSGAQAIGPVLQGFAKPVSDLSRG
                     ASVDDIVATGSLLLAMSTAAERR"
     gene            complement(2035..3126)
                     /locus_tag="AC16A0_01690"
     CDS             complement(2035..3126)
                     /locus_tag="AC16A0_01690"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR3104070.1"
                     /translation="MKILVLNAGSSSLKFAVYGPGLALLVKGQVSGIGSKPRLEKDGQ
                     AVREVPEVTTPTEALLVAAEWISDQGHEPALFNGIGHRIVHGGTRYVAPVAVDDAIQH
                     DLEQLRALAPLHLPFGMGVLREMRRVAPEVPNIACFDTAFHATQPELETRLPIPRDYF
                     DRGYRRYGFHGLNYQHVVEALPRLTGQPLPRRLLAAHLGSGASMCAILEGRSVATTMG
                     FSTADGLVMGTRTGSIDPGVLVALMRDDHLSLDQLEDLLYRRSGLLGLSGISPDMRVL
                     LASDRPEAREAVDYYCYSAARHAASLAPALGGLDAIVFTGGVGENAAPVRAKILAHLA
                     WLKVPASHVHIVPADEERTIARDVTSLLG"
     gene            complement(3165..>4114)
                     /locus_tag="AC16A0_01695"
     CDS             complement(3165..>4114)
                     /locus_tag="AC16A0_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3104071.1"
                     /translation="GTRLGPFGTLPQHGLDYWPKNAKIIQIDSDHKMLGLVKQISVGI
                     VGDAKDSAKALSYRLANRKLACAGNADERKKRIKSEKDAWEKELNDWTQEKDAWSLEI
                     AKGSPYMHPRQMLRELEKAMPKNAMVSTDIGNICSVSNSYLRFTQPNSMFAAMSFGNC
                     GYALPSIIGAKVAAPDRPAVAYVGDGAWGMSFGEILTCVREDIPVTAVVFHNKQWGAE
                     KKNQVDFYDTRFLGVNLKNPSWAEVARSLGAEGVTVDNLGGVGEALDKACDAQKKGKT
                     TVIEVMVTQELGDPFRRDALKYPSRVLEKYKHTDVKAPT"
BASE COUNT          651 a         1318 c         1398 g          747 t
ORIGIN      
        1 gcatctggaa ggatgacagc ctgcggcgac ggcgaggttg agactttgag gatggccgtg
       61 tccggcggcg cggaggccgg ctggatcgtc ggggtgccgt cgccgatctg gaccttgaat
      121 tccttgatcg gcgtcacgag ctcgccggtc gagggcgcga atcccgccac ggtcaggtca
      181 aaactgccct cttgcggcga ggtcgtgacg aggctgacgc cggagagttt cgcgtgcggc
      241 agttcccagg tgttgtcggc ggccttggta ccggccgtta gataagcgct ttccggcacg
      301 cccgagatct tgaggatcag gtcctgtccc tcgaaggccg gctccgcaga cagggccagc
      361 ggaatctggc tgttggcttc gcccgccgcg tcgctcacat tgagtgtcgc gatcgggatg
      421 gtcttcttgg tgatcaccgt ttcggcggcc gcagccggag cggctgtcgc cgtcaccagc
      481 ttcggcaggt cggccatggc ctgatcgggc tggccggaca caggcgcagc ttcgggcgcc
      541 gtcattgcag agatcatcgg tcctgcctgc gatccgatga agcccgcacc gccgcctgct
      601 acgattgccg ccagtgccgc gatgacatag gtcttgcgca gcagcgaggg attggcggaa
      661 ggcgcttcca ttgcccgcgc ggccgcacgg gcttcgagct tggcgccgaa ttcgaagttc
      721 cggggcgcca tgggaaggcg ccgcgcttcg gtcttggcgg gcatttccgg tcggcgcacc
      781 ggttcggagt cgggcgcagg gcggcgcagt ccttccaccg tttccctttc gaagatccac
      841 tgcggatcgg ggtggacgct ctcgtcctcg gtttcagaga cggattccgg ccattgctgg
      901 gaattgttca ctgcatccct cctgcgtgat tggtctccgc tgttcccgga gtcccgattg
      961 cccattccgc cgtcggagcg gaccctgatc cgcgaatgtg gcatgggccg ggcaaaactg
     1021 tgacacttcg ggtacggacg attcgcgaag aaacgatgga tttcggcgtc aactcctgct
     1081 ggactggcac catcagcgag gggcaatgcg ggcaattgac gaggccatgg ccaggatccg
     1141 gggacggaag ctccggctgg tcatgccgga gggcggtgat gaacgcatcc ggatggcggc
     1201 gcagcggctt gtgtcggaag atctcgcacg tcccatcgtc ttctccgagc cggatattcc
     1261 agaggccgat gggcggcaca tcgcattgat ccgtgcacgg cgcgaaaagc tgaccgatgc
     1321 catggcggcg cgcctgctga agaagccgct ctatcgcgcc ggggcgatgg ttgccgcagg
     1381 cgaggccgag gcaatgctcg ccggtgcggc gaatccgacg gcgcgcgtca tcgaggccgc
     1441 gctcatgacc atcgggttag cccccggcat ttcgattccg tcgagcttct ttctgatgca
     1501 gtggcccgat cggcgcctga tctttgccga ttgcgcggtg aatgttcagc ctacggccga
     1561 ggaactggcg gccatcgcgc gggcctcggc tgagagtgca aggcgcgtgc tgggcgagac
     1621 gccgcgcgtg gcgctgctct ctttctcgac caagggcagc ggcaatcatc cggatgcgga
     1681 caaggtcgtt gccgcgctcg cgctggcccg cgcgcaggcg ccgggcctgc tcatcgacgg
     1741 cgagctgcag ggcgatgccg cactgtcggc ggccgtcgcc gcgcgcaaga tggcagagcc
     1801 ggggcccgtg gccggtcagg cgaatgtcct cgtctttccc gatctcgatg ccggcaacat
     1861 tgcctacaag ctcacgcaat atctgagcgg ggcgcaggcc atcggaccgg tgctgcaggg
     1921 cttcgccaag ccggtctcgg acctctcgcg cggcgccagc gtcgatgaca tcgtggcgac
     1981 gggatcgctg ctcctcgcca tgtcgactgc agcagaaaga cgatagagcg gcgctcagcc
     2041 gagcagtgac gtcacatccc gcgcgatggt gcgttcttcg tccgcgggaa cgatgtgcac
     2101 atggctcgcc ggaaccttca gccacgccag atgcgccagg atcttcgcgc gcaccggcgc
     2161 ggcattctcg cccacgccgc cggtaaagac gatggcgtcg aggccgccca gcgccggcgc
     2221 cagcgaggcg gcgtggcgcg cagcggaata gcagtagtag tccaccgcct cgcgcgcctc
     2281 cggacgatcg cttgccagga gcacgcgcat gtccggcgag atccccgaca ggcccagcag
     2341 gccgctcctg cggtagagca ggtcctccag ctggtcgagc gagagatggt cgtcgcgcat
     2401 gagggcgacg agcacgcccg gatcgatcga cccggtgcgt gtgcccatga ccaacccgtc
     2461 tgcggtcgag aaacccatgg tggtggcaac gcttctgcct tcgaggatcg cgcacatgga
     2521 ggctcccgat ccgagatggg cggccagcag gcgccttggc agcggctgtc cggtgagccg
     2581 gggcagggcc tcgaccacat gctgatagtt caggccgtgg aacccatagc ggcgatagcc
     2641 gcggtcgaag tagtcacgcg gaatgggcag ccgcgtttcg agttccggtt gggtggcatg
     2701 gaaggccgtg tcgaagcagg cgatgttggg aacctcgggc gccacgcggc gcatctcgcg
     2761 caggacaccc atcccgaagg ggagatgcag cggggcgagc gcgcgcaact gttcgaggtc
     2821 gtgctgaatc gcatcgtcca ccgccacggg cgccacgtag cgagtcccac catgcacgat
     2881 gcgatgaccg atgccattga acagcgccgg ctcgtggccc tggtccgaga tccattcggc
     2941 ggccaccagc agggcttccg tcggcgtcgt cacttcgggc acctcgcgga cggcctgtcc
     3001 gtccttctcg agccggggct tcgagccgat gcccgagacc tgacccttca ccagcagcgc
     3061 caggcccggg ccatagaccg cgaacttcaa cgatgacgaa ccggcattga ggacgaggat
     3121 cttcattcat ccgtctccgg cgcgactccg tgtggacgac gccgtcaggt gggggccttc
     3181 acgtcggtat gcttgtactt ctcgagaacg cggctgggat acttgagcgc gtcgcggcgg
     3241 aaggggtcgc ccagttcctg ggtcaccatc acctcgatca ccgtggtctt gcccttcttc
     3301 tgcgcgtcgc aggccttgtc cagcgcctcg cccacgccgc cgagattgtc cactgtcacg
     3361 ccttccgcac ccagcgagcg ggcaacttcg gcccagctcg ggttcttgag gttcacgccc
     3421 aggaagcggg tgtcgtagaa atccacctgg ttcttctttt cggcacccca ctgcttgttg
     3481 tggaagacca ctgcggtcac cggaatgtcc tcgcgcacgc aggtcaggat ctcgccgaaa
     3541 ctcatgcccc aggcgccgtc gcccacatag gccacggccg ggcggtcagg ggccgccacc
     3601 ttggcgccga tgatcgaggg cagggcatag ccgcaattgc cgaagctcat ggcggcgaac
     3661 atcgagttcg gctgggtgaa gcggagatag gaattcgaca ccgagcagat gttgccgatg
     3721 tcggtcgaga ccatggcgtt cttcggcata gccttctcga gttcgcgcag catctggcgc
     3781 ggatgcatgt agggcgatcc cttggcgatc tcgagcgacc aggcgtcctt ctcctgcgtc
     3841 cagtcgttca gttccttctc ccaggcgtcc ttctcggact tgatgcgctt cttgcgctcg
     3901 tcggcattgc cggcgcaggc gagcttgcgg ttcgccaggc gataggacag ggccttggcg
     3961 gagtccttgg cgtcacccac gatgcccacc gagatctgct tcacgaggcc cagcatcttg
     4021 tggtcgctgt cgatctggat gatcttggca ttcttcggcc agtagtcgag gccgtgctgc
     4081 ggcagggtgc cgaagggtcc aagccgcgtg ccga
//
LOCUS       DBNGVR010000077         3020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1597822, whole genome shotgun sequence.
ACCESSION   DBNGVR010000077 DBNGVR010000000
VERSION     DBNGVR010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1597822"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="AC16A0_01700"
     CDS             complement(<1..250)
                     /locus_tag="AC16A0_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3104072.1"
                     /translation="MPSFSYILVSLVILLTPGPTNTLLAAAGAALGLRRALILPLAET
                     LGYGIAISVFFAMAATLADHPLALAWLRGLAALWLVVVG"
     gene            549..2207
                     /locus_tag="AC16A0_01705"
     CDS             549..2207
                     /locus_tag="AC16A0_01705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3104073.1"
                     /translation="MTAADILITNARILTMDGARPRAEALAITGNRITRIGSRDDVAG
                     LKAKHTRVIDAQMKTVMPGIIEGHVHLFGGSVELDTLMLNGIMGFDAISESVSQYRKT
                     RPKDAVLLATGIIHEAFGEPITRQLLDRIVADIPFIVGCFDHHTMWANTKALEAAGIM
                     RGRDLPVGNEIVLDHNGIATGELREPAAYMLIQALTPTGGREWLGMTTGENPNPPATA
                     AQRATDIAFFRQGIAYAASLGITSMHNMDGNWYQLELLQEMLDRGELNARVEIPFHQK
                     NYFELSRVDEAVDMRAAYNGDMLHCHRVKIFIDGVLETLTALMIDDYPGHPGNKGAPL
                     FTAADFDALVTRADKHGFQISTHAIGDGAIRRTLDGYAAAQKANGRRDSRHRIEHIEL
                     IDPADIPRLKELGVIASLQPVSAPTVPGNPLEPILTRVGDKLPYAYAWQLLREAGAVV
                     AFSSDWPVAPLNPFQGIQAALTARPLRADCRPQAQTLMDTLRSFTADGAYMEFMEDRK
                     GTLKEGYLADVIVLDADLEATAPDRIATVLPVTTICDGRVTFDR"
     gene            2268..>3020
                     /locus_tag="AC16A0_01710"
     CDS             2268..>3020
                     /locus_tag="AC16A0_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3104074.1"
                     /translation="MSSTYRQMLQQAAALHQQGRIGEALKLYNTVLAVDAGNLDAMHL
                     KGLALFHMGQFSEAIGQLTTVIRRNRNFPPALNNRGLAYQALGKRNEALQDFEKAVSL
                     QPGFAEALCNKANVLRELGRLDAATEACLAAIKLRPGIPEFHKGLGVIYKDKRDWTAA
                     LNQFDQAAKLRPSYVEAIANRANALKELGHTEEALAAYDHALRLRPGQAELHYNRANA
                     LKDLGRLAEAVAGYDRAIGLNAIYAEAFCNRGN"
BASE COUNT          587 a         1025 c          937 g          471 t
ORIGIN      
        1 ggccgaccac gaccagccag agcgcggcga gccccctcag ccatgcaagc gccagcggat
       61 gatcggcgag agtcgcggcc atggcgaaga aaacggaaat ggcgatgcca tagccgaggg
      121 tttcggccaa cggcaggatc agggcacggc gcagcccgag ggcggcacct gccgcggcaa
      181 gcagcgtgtt ggtcggtccc ggcgtcagca ggatcacaag ggacacgaga atgtagctga
      241 agctcggcat gccggatcaa gtgctcaggg ccttcggggg ccatgcccac acaagggcgg
      301 gcatgacaaa gaaaggccgc tctcgcgagc ggcccttccc gtgagatgcg tgtgacggca
      361 tccccgatcc cgtgacgtca ggcccgcgct ccgcggaggc tgacgatccg aacgaggctt
      421 gcgccacacg cagtgaactc actcgacatc ggatcacctc ctttccttgt tgacgatgca
      481 ccaaacgtga ggccttgccc ctgcttcgtc aagcgggcat tttcgggccc caaccgcaag
      541 gacccgccat gaccgcagcc gacatcctga tcaccaacgc ccgcattctc accatggacg
      601 gggcgcgccc ccgcgccgag gcgctggcca tcaccggcaa tcgcatcacc cggatcggca
      661 gccgggacga cgtcgcgggc ctcaaggcca agcacacgcg ggtgatcgac gcgcagatga
      721 agaccgtcat gcccggaatc atcgagggcc atgtccacct gttcggcgga tcggttgagc
      781 tcgatacgct aatgctcaat ggaatcatgg gtttcgatgc aatctcggaa tcagtttctc
      841 aataccgcaa gacgcgcccc aaggacgccg ttctgctggc cacgggtatc atccacgagg
      901 cctttggcga gcccattacc cggcagctgc tggaccgcat cgtcgccgac atccccttca
      961 tcgtcggctg cttcgaccat cacaccatgt gggccaatac caaggcgctg gaggcagccg
     1021 gcatcatgcg cggccgcgac ctgccggtgg gcaacgagat cgtgcttgac cacaatggca
     1081 tcgccaccgg cgagttgcgc gagcccgccg cctacatgct gatccaggcg ctgaccccca
     1141 ccggcgggcg cgagtggctg ggcatgacca ctggcgagaa ccccaacccg ccggcgaccg
     1201 cagcccagcg cgccaccgac atcgccttct tcaggcaggg catcgcctat gccgcctcgc
     1261 tcggcatcac atcgatgcac aacatggacg gcaactggta tcagctggaa ctgctgcagg
     1321 agatgctgga tcgcggcgag ctcaacgcgc gggtggagat ccccttccac cagaagaact
     1381 acttcgagct gtcgcgcgtc gacgaggcgg tggacatgcg cgcggcgtac aacggcgaca
     1441 tgctgcactg ccaccgcgtg aagatcttca tcgacggcgt gctggaaacg ctgactgcgc
     1501 tgatgatcga cgactatccg ggccatcccg gcaacaaggg ggcgccgctc ttcacggccg
     1561 cggacttcga tgcgctggtg acgcgtgccg acaagcacgg cttccagatt tccacccatg
     1621 cgatcggcga cggcgccatc cgccgcacgc ttgatggcta cgcggctgcg cagaaggcca
     1681 atggcaggcg cgactcgcgc caccgcatcg agcatatcga actgatcgat ccggccgaca
     1741 ttccgcgcct gaaggaattg ggcgtgatcg cctcgctgca gcccgtctct gcgcccaccg
     1801 tgccgggcaa tccgctggag cccatcctga cgcgcgtggg cgacaagctt ccctatgcct
     1861 atgcctggca attgctgcgc gaggcgggtg cggttgtcgc cttctcgtcg gactggccgg
     1921 tggcgccgct caatcccttc cagggtatcc aggccgcgct gaccgcaaga cctctgcgcg
     1981 ccgattgccg gccgcaggcc cagacgctga tggacacgct gcgcagcttc accgccgacg
     2041 gcgcctacat ggagttcatg gaagaccgca agggcacgct gaaggaaggc tatctcgctg
     2101 acgtgatcgt gctcgacgcc gatctcgagg cgacggcgcc cgaccgcatt gccacggtcc
     2161 tccctgtcac caccatctgc gacggccggg taaccttcga ccgctgagtg aacattgtcg
     2221 cacggccggt tcctgccacc gcagccagcc aatcggagaa atctgcagtg tcatcaacct
     2281 acaggcaaat gctgcagcag gcggccgctc tgcatcagca aggccgcatc ggcgaagccc
     2341 tgaagctcta caacacggtt cttgccgtcg atgcggggaa cctcgatgcg atgcacctga
     2401 agggtctggc cctcttccac atgggtcagt tcagcgaggc catcggccag ctgacgacgg
     2461 tcatccggcg caaccgcaac tttccgcccg ccctcaacaa tcgcgggctc gcctatcagg
     2521 cgctgggaaa gcgcaatgag gccctgcagg acttcgagaa ggcggtttcg ctgcagccag
     2581 gttttgcaga ggccctgtgc aacaaggcca atgtcctgcg cgagctgggc cgtcttgatg
     2641 cggcaacgga ggcctgcctc gcggccataa agctgcggcc gggtataccg gagttccaca
     2701 aaggcctcgg cgtgatctac aaggacaagc gcgactggac ggcggccctg aaccagttcg
     2761 accaggcggc aaagctgcgc ccttcctatg tcgaagctat cgcgaacagg gccaatgccc
     2821 tgaaggaact cggacacacc gaggaggccc tggccgccta tgaccatgcg ctgcggttgc
     2881 ggcccgggca ggccgagctc cactacaatc gcgccaatgc gctcaaggat ctgggccggc
     2941 tcgcagaggc cgtggcaggc tacgaccggg cgatcggcct gaacgccatc tatgcggagg
     3001 ccttctgcaa cagaggcaac
//
LOCUS       DBNGVR010000078         6469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_617060, whole genome shotgun sequence.
ACCESSION   DBNGVR010000078 DBNGVR010000000
VERSION     DBNGVR010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_617060"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /locus_tag="AC16A0_01715"
     CDS             <1..1827
                     /locus_tag="AC16A0_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3104075.1"
                     /translation="RLTVTGALMKVYEGPGAIAQMAKARVLPVRIEGALYTPFSRMRG
                     KLRLRWFPKITITFLPPVKFDPPEGVRGAALREHQADRLYDVMTDMVFRTSNIDQPLF
                     DALIDARHTHGGNHEIIEDIQRQPVTYNRLIMGSFILGRRIAAASQGQPQVGVLLPNA
                     AGTLVTLFGLYAYGRVPAMLNFSTGATNMAAACSAAQISTIVTSRRFIEMAEMEETLK
                     IVSEGRRIIYLEDLRQSLTLGDKLYGLWARNFSKTALRLAGATRDARTPAAILFTSGS
                     EGVPKGVVLSHRNLNANRYQAAARIAFTAQDIVFNALPMFHAFGLTGGSLLPVLAGVR
                     TFFYPSPLHYKVVPELCYDTNATILFGTDTFLMGYARNAHPYDFFNLRMVVAGAERVK
                     PETREIWMEKFGLRILEGYGATECSPVIAVNTPMHFKTGTVGRLLDQVQYRLEPVEGI
                     TEGGRLFVKGPNVMLGYLRADNPGVLEAPPDGWYDTGDIVSVDDREFVTILGRAKRFS
                     KIAGEMVSLSSVESKVQAAFPDQQAAVVAVPDAKKGEQLVLFTTDAKLERKTLSDALK
                     AAGATELMIPKTIIAVKELPVLGSGKTDYVSLNRMAREQVKP"
     gene            1824..3089
                     /locus_tag="AC16A0_01720"
     CDS             1824..3089
                     /locus_tag="AC16A0_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR3104076.1"
                     /translation="MSLALDADDHARLEGREGKAMQLAMRLVVQAAQNLDAKRLIPVT
                     FAHVDACFYTGQAHVDFVRFLLENGARLSIPTWTNNGVVSLADPTLRPAAEDPDFVAG
                     AGEVMRLYAELGCVPTWTCAPYQLPGGPKLGDHIAVGESNAVSYYNSAVGARTNKYGD
                     YLDVACALVGKAPFAGLHTDEGRKARLRFACEDLPDAWRREDIFAHLLGHHVGRIAGR
                     RVPVMTGLPAETTADNLKAISSAVAASGGVELWHGVGVTPEAPSLDAVFGGGDCHQVT
                     AEDLTMAHRDLSTARDGPLDAVALGTPHFSISEFADLVQLLDGRKTKPGLVFLVSTSR
                     AVRQMAAAKGWIAELERAGVEVPVDVCTYYSPRVRGMRGRIMSNAAKWAYYAPGMLGV
                     EVAFGSLRECVESAVRGEVWRDPALWIRP"
     gene            3086..3499
                     /locus_tag="AC16A0_01725"
     CDS             3086..3499
                     /locus_tag="AC16A0_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014091.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIR3104077.1"
                     /translation="MIRSRVLKAAAAEGPVLILDEPLSFWGAFEPRSGVILDVHHPQR
                     GACLAGTILLMRESKGSGSAPGGIAEAIRLGNAPAGIILVTPDINLAVGAEVAETLYG
                     RGCAVVAVTEDDFTALCRAARLSISVTGEIRDETV"
     gene            3531..4706
                     /locus_tag="AC16A0_01730"
     CDS             3531..4706
                     /locus_tag="AC16A0_01730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3104078.1"
                     /translation="MRFAKVTQRLAGLGSEKWAVHIEGRRRADLGEPMIFLSIGEPDA
                     PPPAAIFDVAERQMRAGRTRYSNGRGEPDVLRALSRLYTERTGRSISTSQFLFLPGTQ
                     TALYVAMMVLVELGDEVLMADPYYATYEGVIAAAGGVAVPVRVDPDHGFHLKAAALEK
                     AITPKTRVLLMNTPSNPTGAVFTREEIARIGKVCEQHDLWIICDEVYASLTYGNTVFA
                     SPFDRVELEKRTVVVSSVSKSHAMPGFRCGWIASSEEFCAAALPVSETMLFGSQPFLE
                     DATAFALDNHFSELDAMKHAYERRAQVLVEGLRASKTVSARMPEGGMFVMVDVRKTGL
                     SGRAFAERLLDEENVVTMPGESFGAGGAGHLRVALTVDESQIAEACRRIARLADRLC"
     gene            5013..5423
                     /locus_tag="AC16A0_01735"
     CDS             5013..5423
                     /locus_tag="AC16A0_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR3104079.1"
                     /translation="MAETGLAGLSAPSIADFERLAAEAFDGLPADFRALCGNIVIQVE
                     DFPTDEVQAEMRAGPFDLLGLFQGIGLAQDQAVPETGRLPNMVFIYRRAILDYWAEHA
                     ETLGAIVTHVLVHEIGHHFGLSDDDMYAIERRAG"
     gene            5420..5617
                     /locus_tag="AC16A0_01740"
     CDS             5420..5617
                     /locus_tag="AC16A0_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104080.1"
                     /translation="MTPDQEESRRILERMEREREKLFHATPTADNDDDQIERLGKRIA
                     RILGPLIAVGLMAYLFLTYLG"
     gene            5685..>6469
                     /gene="argC"
                     /locus_tag="AC16A0_01745"
     CDS             5685..>6469
                     /gene="argC"
                     /locus_tag="AC16A0_01745"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3104081.1"
                     /translation="MTSVFIDGEAGTTGLQIRDRLKSRPDVTLVSIDPDRRKDPSARR
                     ELLNSVDIAILCLPDDAAREAVALVDPASKTRLIDASTAFRVSEGWAYGMAEMSEAHR
                     AQIAASSRVANPGCYPQGLIALVRPLIDAGIMSADYPVTYNAVSGYSGGGKKMIESYE
                     AAGSTAIPYLPYGLTFAHKHLPEMQRYAMLAHAPVFQPAVGNYAQGMLGAVPVFGELL
                     VKRATGEAVRDCLAEAYDGSPVIEVAPFAEIERSESISPEVLN"
BASE COUNT         1098 a         2053 c         2087 g         1231 t
ORIGIN      
        1 cgcctcaccg tgacgggcgc attgatgaag gtctatgaag ggccgggcgc cattgcgcag
       61 atggccaagg cgcgcgtgct gccggtaagg atcgagggcg cgctctacac gcccttctcc
      121 cgcatgcgcg gcaagctgag gctgcgctgg ttccccaaga tcaccatcac cttcctgccg
      181 cccgtcaagt tcgatccgcc ggagggagtc cgcggtgcag cgcttcgcga gcaccaggcg
      241 gaccgcctct atgatgtgat gaccgacatg gtcttccgca cctccaacat cgaccagccg
      301 ctgttcgatg cgctgatcga tgcgcggcac acccatggcg gaaaccacga gatcatcgag
      361 gatatccagc gccagccggt cacctataac cggctgatca tgggctcctt catcctcggc
      421 cgccgtattg ccgctgcgtc gcaggggcag ccgcaggtcg gtgtccttct tcccaatgcc
      481 gcaggaaccc tggtgacgct cttcggcctc tatgcctatg gccgcgtacc tgcaatgctc
      541 aatttctcca cgggtgcgac caacatggcg gcggcctgct cggcggcgca gatatccact
      601 atcgtgacgt cgcggcgatt catcgagatg gcggagatgg aggagacgct gaagatcgtc
      661 tccgaaggac gcaggatcat ctatctggag gatctccgcc agtcgctgac cctgggcgac
      721 aagctctacg gactatgggc ccggaacttt tcaaagaccg ccttgaggct ggcaggcgcc
      781 acgcgcgatg cccggacgcc ggcggcgatc ctcttcacgt cgggctcgga aggcgtgccg
      841 aagggcgttg tgctgtcgca tcgcaacctc aacgccaacc gctatcaagc cgcggcgcgc
      901 atcgccttta cggcgcagga cattgtcttc aatgcgctgc ccatgttcca tgccttcggg
      961 ctgaccggcg gctccctgct tccggtgctg gcaggtgtcc gtaccttctt ctatccctcg
     1021 ccgctgcact acaaggtggt tccggaactc tgctacgaca ccaacgcaac catcctgttc
     1081 ggcaccgata ccttcctcat gggctatgcc cgcaatgccc atccctatga cttcttcaac
     1141 ctgcgcatgg tcgtagcagg cgccgagcgg gtgaaacccg agacccgcga gatctggatg
     1201 gagaaattcg gcctgcgcat cctcgaaggc tacggtgcga ccgagtgttc gcccgtcata
     1261 gcagtgaata cgccgatgca cttcaagacc ggaacggtcg gacgcctgct cgaccaggtt
     1321 cagtaccgcc tcgagccagt ggaaggcatc accgaaggcg gtcggctctt cgtgaaaggt
     1381 cccaacgtga tgctgggtta tctgcgcgcc gacaatcccg gcgtgctcga ggcgccgccg
     1441 gacggctggt acgacacggg cgatatcgtc agcgtcgatg accgcgagtt cgtcaccatc
     1501 cttggccggg ccaagcgctt ctccaagatc gcaggcgaga tggtgtcgct gtcctcggtc
     1561 gaatcgaagg tgcaggcggc cttcccggat cagcaggcgg ccgtggtggc ggtgcccgat
     1621 gccaagaagg gcgagcagct ggtgctcttc accaccgatg cgaagctgga gcgcaagact
     1681 ctttccgatg cgctgaaggc cgccggcgcc acagaactga tgattccgaa gaccatcatc
     1741 gcggtcaagg agcttccggt tctggggtcg ggcaagacgg actatgtgtc tctcaaccgc
     1801 atggcgcggg agcaggtgaa gccgtgagcc tggcgctcga cgcagatgat cacgcgcgac
     1861 tggaaggccg cgagggcaaa gcgatgcagc tggccatgcg gctggtggtg caggcggccc
     1921 agaacctgga tgccaagcgg ctgatcccgg tcaccttcgc gcatgtcgat gcctgcttct
     1981 acacggggca ggcccatgtc gacttcgtgc gcttcctgct ggagaatggc gcgcggcttt
     2041 cgatcccgac ctggaccaac aatggcgtgg tgagcctggc cgatccgacg ctcaggccgg
     2101 cggcggaaga tcccgacttc gtggccggcg cgggcgaggt gatgcggctc tatgccgagc
     2161 tggggtgcgt gccgacctgg acctgcgcgc cctaccagct gcccggcggt ccgaagctgg
     2221 gcgatcacat cgcggtggga gagtcgaacg cggtctccta ttacaattcc gcggtgggcg
     2281 cgcgcaccaa caagtacggc gactaccttg atgtcgcatg tgcgctggtc ggcaaggcgc
     2341 catttgccgg acttcacacc gatgaaggcc gcaaggcccg gctgcggttt gcctgcgaag
     2401 acctgccgga cgcctggcgg cgcgaggaca ttttcgccca tctgctgggc catcacgtgg
     2461 gccgcatcgc cgggcggcgc gtgccggtga tgacggggct gccggcagag acaacggccg
     2521 acaatctgaa ggccatcagt tccgccgtgg cggcctccgg cggcgtcgaa ctctggcatg
     2581 gggtgggcgt cacgcccgag gcgccctcgc tcgatgcggt gtttggcggt ggagactgcc
     2641 atcaggtcac cgccgaggac ctgacgatgg cgcatcgcga cctctcgacc gcgcgcgatg
     2701 gcccgcttga cgccgttgct ctgggcacgc cgcatttctc gatcagcgag ttcgcagacc
     2761 tcgtgcagct tctcgatggc cggaagacca agccggggtt ggtcttcctg gtttcgacca
     2821 gccgggcggt acgccagatg gcggcggcca aaggctggat cgccgaactt gaacgggcgg
     2881 gcgtggaagt gccggtcgat gtctgcacct actattcgcc gcgggtgcgc ggcatgcgtg
     2941 gccgcatcat gagcaacgcc gcgaagtggg cctactacgc accgggcatg ctgggcgtgg
     3001 aggtggcctt cggttcgctg cgcgaatgcg tggagagtgc ggtgcgtggc gaggtctggc
     3061 gcgatcctgc cttgtggatc aggccatgat ccggtcccgc gtgctgaaag cggctgccgc
     3121 ggaggggccg gtcctgatcc tcgacgagcc cttgagtttc tggggcgcct tcgaaccccg
     3181 cagcggcgtg atcctcgatg tgcatcatcc gcagcgtggc gcctgcctcg ccggcaccat
     3241 cctgttgatg cgcgagagca agggttcggg ctcggcgcct ggtggcattg ccgaggccat
     3301 ccggctcggc aatgccccgg caggcatcat cctcgtgacg cctgacatca acctggccgt
     3361 tggggcggag gtggccgaaa cgctttatgg ccgcggctgc gccgtcgtgg ctgttaccga
     3421 ggacgatttc actgcccttt gccgcgcggc gcggctgagc atttccgtca caggtgaaat
     3481 ccgcgacgaa accgtgtaac aggcggtccg gaatcggaac aggtgaattc atgcgctttg
     3541 cgaaggtgac tcagcggctt gccgggctgg gttcggagaa gtgggccgtg catatcgaag
     3601 gccggcgtcg ggccgacctt ggcgagccca tgatcttcct ttcgatcggc gaacccgacg
     3661 cgccgccgcc agccgccatc ttcgatgtgg ccgagcgtca gatgcgggcc ggtcgcacac
     3721 gctattccaa cggacgcggc gagcccgatg tcctgcgcgc gttgtcgcgc ctctataccg
     3781 aacggaccgg ccggagcatc tccaccagcc agttcctgtt tctgccgggt acgcagacgg
     3841 cgctctatgt cgcgatgatg gtgctggtgg agcttggcga cgaagtgctc atggccgacc
     3901 cctactatgc gacctacgag ggcgtgatcg cggctgcggg cggcgtggcg gttccggtcc
     3961 gggtcgatcc ggatcatggc tttcatctga aggccgcggc cctcgagaag gcaatcacgc
     4021 ccaaaacccg cgtgctgctg atgaatacgc cgagcaatcc gaccggcgcc gtcttcacgc
     4081 gcgaggaaat cgcccggatc ggcaaggtct gcgagcagca cgatctctgg atcatctgcg
     4141 acgaggtcta tgcatcgctc acctacggca atacggtctt cgcttcgccc ttcgacaggg
     4201 tggaactgga gaaacgcacc gtcgtggtgt cctcggtgtc gaagtctcat gccatgccgg
     4261 ggttccgctg cggctggatc gcttcgtccg aggaattctg cgcggcggct cttccggtga
     4321 gcgaaaccat gctcttcggt tcacaaccct tcctcgagga tgcaaccgct ttcgcactgg
     4381 acaaccattt ttcggaattg gatgccatga agcacgccta tgagcgccgg gcgcaggtcc
     4441 tcgtggaagg cctgcgggca tcgaagacgg tctcggcccg catgcccgaa ggtggcatgt
     4501 tcgtcatggt ggacgtgcgc aagaccgggc tctccggtcg ggcctttgcc gagcgcctgc
     4561 tggacgaaga gaatgtcgtg accatgcccg gcgagagctt tggagcaggc ggggccggtc
     4621 atctgcgggt ggcgttgacc gtggacgaat cgcagattgc cgaagcttgc cgccggattg
     4681 cccgtctggc cgaccgcctt tgctaattgt acagtgattt caaaggcctc tgtaccgctt
     4741 tggagcgcag aattaacggt tgatgaacaa actgttaagc tttatgtcac ccgcgtggca
     4801 gtaaagtgat tcataagttc aaccattgct gcaaaccttt gcggggtatc gacgtgggtt
     4861 ctacagttca ggcggatgcc aaattcctcc gccgtgtttt tctcggcctg gttctcttga
     4921 ccttggccac tggctttctg gcaggcctga cgacggtgca ggccatgacc gacaagaccg
     4981 ggacctgcgt cctgctctgc caatagggcg ccatggcgga gaccgggctc gcgggcctct
     5041 ccgccccttc catcgcggat ttcgaacggc tcgccgccga ggcctttgat gggcttccgg
     5101 ccgattttcg tgcgctttgc ggcaatatcg tgatccaggt ggaagacttt ccgaccgatg
     5161 aggtccaggc cgagatgcgg gccggcccct tcgatctctt ggggctcttc cagggcatcg
     5221 gcctggcgca ggatcaggca gtgccggaaa ctggccgtct gcccaacatg gtctttatct
     5281 atcgccgggc aatcctcgac tactgggctg aacacgcgga aaccctgggc gccattgtca
     5341 cccatgtctt ggtgcatgag atcgggcatc atttcggcct gtccgacgat gacatgtatg
     5401 ccatcgagcg gcgtgcggga tgacaccgga ccaggaagaa tcccgccgca tcctcgagcg
     5461 catggaacgc gaacgcgaaa aactgttcca cgccacgccg accgccgaca atgacgatga
     5521 tcagatcgaa cggctgggca agcgcatcgc ccgcattctc ggtccactga ttgccgtcgg
     5581 cctgatggcc tatctcttcc tgacatatct gggttgacaa ggcccggcac cggaacgggc
     5641 gctgatgttg cgcccctgcc gccccgcctg tatgaagccc cgccatgacc agtgtattca
     5701 tcgatggtga agccgggaca accgggctgc agatccgcga ccggctgaag agccggccgg
     5761 acgtgacact cgtgtcaatc gatccggatc gccgcaagga tccctccgcc cggcgcgagt
     5821 tgctgaattc cgtcgatatc gccatcctgt gcctgccgga cgatgcggcc cgggaggcgg
     5881 tggccctggt cgatccggcc tcgaaaaccc ggctgatcga tgcctccacc gccttccgcg
     5941 tgtccgaagg ctgggcctat ggcatggccg aaatgtcgga ggcgcaccgc gcccagatcg
     6001 ccgcgtcatc ccgcgtggcg aatcccggct gctatccgca ggggctcatt gcccttgtcc
     6061 gtccgctgat cgatgcgggg atcatgtcgg cggattatcc cgtcacctac aacgcggtgt
     6121 ccggctattc gggcggcggc aagaagatga tcgagtccta cgaggccgcc ggctcgactg
     6181 ccattcccta tctgccttat ggtctgacat tcgcgcacaa gcatctgccc gaaatgcagc
     6241 gctacgccat gctggcgcat gcaccggtgt tccagcccgc ggtcggcaac tatgcccagg
     6301 gcatgctggg cgcggttcct gtgttcggcg aacttctcgt caagcgcgcc acaggtgaag
     6361 cggttcgcga ctgcctggcc gaggcctatg atggatcgcc cgtcatcgag gttgcgccct
     6421 ttgccgagat cgagcgctcg gagtcgatca gccccgaggt gctcaacaa
//
LOCUS       DBNGVR010000079         3551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_617924, whole genome shotgun sequence.
ACCESSION   DBNGVR010000079 DBNGVR010000000
VERSION     DBNGVR010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_617924"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="AC16A0_01750"
     CDS             <1..1004
                     /locus_tag="AC16A0_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HIR3104082.1"
                     /translation="YGSQMLSLGGVRHLTGGSKKEGRVTCESLLALCNCQPVEVEVDG
                     GARVVIAAGQPPVINGQPEERMRVGCGSATIGMFAKQWLGLVDEVVVVDDHITGVLSE
                     HQAGKFLDVSETGVRIKGHRSTPGRYFKVAQPGTGWGGTDLTDPLAILKPFDAKTAWP
                     GLRLFMVSTTGEHFAYFELDEALTPVEKDLPPALRLSVERIAENCEPALSTVLFMGGA
                     GGSLRAGVTENPVRLTRSVKDALTRVTCGGAPVYVWPGGGITFMADVTRLPANAFGYV
                     PTPALVAPIEFTMRREDYRALGGHMDEVLKLSDVETHRGARRVAALPDNPWPLDRKS"
     gene            1001..1870
                     /locus_tag="AC16A0_01755"
     CDS             1001..1870
                     /locus_tag="AC16A0_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HIR3104083.1"
                     /translation="MSRMQARMLPDGRRLHLQDGPIDLVIGAEGRAADVRHAQQAATA
                     RFATILDELCAELELLRRPVDLCPEGSVARRMWAATRAHARSTFITPMAAVAGSVAED
                     VLAAMTAAAPLTRAFVNNGGDIAIHLQPGQRYAIGLVDRPDRPSLFARAGICEDDSIG
                     GIATSGWRGRSFSLGIADAVTVLAETASRADAAATVIANAVDLPGHPAITRVPATQLQ
                     PDSDLGVRPVTRAVGPLRPGEIAAALASGLACAVSLLKDGYIRACALHLAGETRILGH
                     VPLEHDWRLAAYA"
     gene            1863..2447
                     /locus_tag="AC16A0_01760"
     CDS             1863..2447
                     /locus_tag="AC16A0_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HIR3104084.1"
                     /translation="MPDVRIRKFLVSVEEIFHEGGPVAEAPPRRGAVLAVIENPYAGR
                     YHAKIQPFMEDLRPLGLLMARKHVEALGGDAGVIEGYGKGAIVGEAGEIEHGALWHAP
                     GGYAMREVLGGAKAIVPSTKKVGGPGTRLDVPVTHLNASYVRSHFDAIEVGVSDAPRA
                     NEIVLTLVMTTGPRVHARAGGLDVKDVKGQDGLR"
     gene            2444..3028
                     /locus_tag="AC16A0_01765"
     CDS             2444..3028
                     /locus_tag="AC16A0_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HIR3104085.1"
                     /translation="MKARIRKIVTIVDETLSEQGRDVSPPVRRAACVAVIANPFAGTY
                     QEDLTDLIDIGEELGGLLAERAVAALGIAGAAVESYGKAAAVGENGELEHAAAILHPK
                     LGAPFRKVLGKGAALIPSSKKRGSLGVVLDIPLGHKDAAFVRSHFDGMEVRVNDAPRA
                     NEIMVAVAVTTGGRPLPRVGGLMTSEVKGEDGLR"
     gene            complement(3025..>3551)
                     /locus_tag="AC16A0_01770"
     CDS             complement(3025..>3551)
                     /locus_tag="AC16A0_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3104086.1"
                     /translation="FDFETAHHHPAWFKELHGFKNHMPETEEYDISSFVYRARRPFVP
                     DAFQGFIRGSWPGVLRAKGFFWLATRPQWVGELSQAGALVRTEAAGQWWAGIDERHWP
                     TSPEWRAWITSRWDATWGDRRQELVFIGAGMDRDAITRSLDACLVPETTPAFDRWSRL
                     PDPFPAWRREESAA"
BASE COUNT          568 a         1212 c         1206 g          565 t
ORIGIN      
        1 aatacggctc gcagatgctg tcgctcggcg gcgtgcgcca cctgaccggc ggatcgaaga
       61 aggaaggccg cgtcacctgc gaaagcctgc ttgccctgtg caattgccag ccggtggagg
      121 tcgaggtgga cggcggcgcg cgtgtcgtca tcgccgcagg tcagccaccg gtcatcaatg
      181 gacagcccga ggaacgcatg cgcgtcggct gcggatctgc caccatcggc atgttcgcca
      241 agcagtggct tggactggtc gacgaggtgg ttgtggtcga cgatcacatc accggcgttc
      301 tctccgagca ccaggcggga aaattcctcg acgtttcgga aaccggcgtc cgcatcaagg
      361 gccaccgctc cacgccggga cgctacttca aggtggccca gcccggcacc ggctggggcg
      421 gcaccgatct gaccgacccg ctcgccatcc tgaagccctt cgatgcaaag acggcgtggc
      481 ctggcctgcg cctcttcatg gtctcgacca ccggcgagca cttcgcctat ttcgaactcg
      541 atgaagccct gacgccggtc gagaaggacc ttccgcccgc gctgcggctt tcggtcgagc
      601 gcatcgccga gaactgcgag ccggccctgt cgaccgtgct tttcatgggc ggtgccggcg
      661 ggtcgctgcg ggccggcgtg accgagaatc cggtccgcct cacgcgatcg gtgaaggacg
      721 cgctgacgcg cgtcacctgc ggcggggcgc cggtctatgt ctggccgggc ggcggcatca
      781 ccttcatggc agacgtgacg cggcttcccg ccaatgcctt cggctacgtg ccgactccgg
      841 ctcttgtcgc gccgatcgag ttcaccatgc gtcgggagga ttatcgggcg ctcggcggcc
      901 acatggacga ggtgttgaag ctttcggatg tcgaaaccca tcggggtgcc agacgcgtcg
      961 cggcactgcc ggacaatccc tggccgctgg atcggaagtc gtgagccgca tgcaggcaag
     1021 gatgctgcct gatggccgca ggctccacct gcaggatggg ccgatcgatc tcgttatcgg
     1081 cgccgaaggg cgcgccgccg atgtccggca tgcccagcag gccgcgacgg cacgctttgc
     1141 caccatcctt gacgagctct gcgccgaact tgagctgttg cgccggccgg ttgatctctg
     1201 cccggaggga tccgtcgccc ggcgcatgtg ggccgcgaca cgcgcgcatg cacgcagcac
     1261 cttcatcacg cccatggctg ccgtggccgg ttcggttgca gaggacgtcc tggccgccat
     1321 gaccgccgcc gcaccgctga cgcgcgcctt cgtgaacaat ggcggcgata tcgccattca
     1381 cctgcagcca ggccagcgct acgccatcgg cctcgtggat cggcccgacc gcccctcgct
     1441 cttcgcgcgc gccgggatct gtgaggacga cagcatcggc ggcatcgcca ccagcggctg
     1501 gcgcgggaga agcttctcgc tcggcattgc cgatgcggtg acggtgctgg cggagacggc
     1561 aagccgcgcc gatgccgccg ccaccgtgat cgccaatgcc gtcgatcttc ccggccatcc
     1621 ggcgatcacc cgcgtgccgg ccacgcagct gcagcccgac agcgatctcg gcgtccgtcc
     1681 cgtgacccgc gccgtcggcc cgcttcggcc gggcgagatc gccgcggcgc tcgcatccgg
     1741 gctggcctgt gcggtgtcgc tgctgaagga tgggtacatc cgggcctgcg cactgcatct
     1801 cgcgggcgaa acccgtatac tgggccatgt gcccctcgaa catgattgga ggcttgcagc
     1861 ctatgcctga cgtcaggatc cggaagtttc tcgtcagcgt cgaggagatc tttcatgaag
     1921 gtggccccgt cgccgaagcg ccgccgcgcc gcggcgcggt gctggcggtg atcgagaatc
     1981 cctatgccgg ccgctaccat gccaagatcc agcccttcat ggaggacctg cgcccgctgg
     2041 gcctgctcat ggcgcggaag cacgtcgaag ccctcggcgg cgatgccggg gtcatcgaag
     2101 gctatggcaa gggcgccatc gtcggcgagg cgggcgaaat cgagcacggc gcgctgtggc
     2161 atgcgccggg cggatacgcc atgcgcgagg tcctcggcgg cgccaaggcc attgtgcctt
     2221 cgaccaagaa ggtgggcggt ccgggcacgc gtctcgacgt gccggtcacc catctcaatg
     2281 cctcctatgt gcgcagccat ttcgatgcca tcgaggtcgg cgtcagcgat gcgccgcgcg
     2341 ccaacgagat cgtgctgaca ttggtcatga ccaccggtcc gcgcgtgcat gcccgtgccg
     2401 gcggactcga tgtgaaggac gtcaagggac aggatggatt gagatgaaag ccaggatccg
     2461 caagatcgtc accatcgtcg acgaaacgct gagcgagcag ggcagggacg tgtcgccgcc
     2521 ggtccgccgc gccgcatgtg tcgcggtcat cgccaatccc tttgccggaa cctaccagga
     2581 agacctgacc gacctcatcg acatcggcga ggaactgggc gggctgctgg ccgaaagggc
     2641 ggtggcggca ctcggcatcg ccggtgcggc cgtcgagagc tatggcaagg cggcggcggt
     2701 cggtgagaat ggcgagctcg aacatgccgc ggccatcctg catcccaagc tcggcgcgcc
     2761 gttccgcaag gtgctgggca agggcgcggc cctgatcccg tcctccaaga agcgcggcag
     2821 cctcggcgtg gtgctcgaca ttccgctggg ccacaaggat gccgccttcg tgcgcagcca
     2881 cttcgatggc atggaggtgc gcgtcaacga tgcgccgcgg gccaacgaga tcatggtcgc
     2941 cgtcgccgtg accaccggcg gccgccccct cccgcgcgtc ggcggcctca tgacatccga
     3001 ggtcaagggc gaggacgggc tgcgctaggc ggcgctctcc tctcggcgcc aggcagggaa
     3061 aggatcgggc agcctggacc acctgtcgaa ggccggcgtg gtctcgggca caaggcaggc
     3121 atcgagactc cgcgtgatcg cgtcccggtc catgccggcg ccgatgaaca ccagctcctg
     3181 gcgccggtca ccccaggtgg cgtcccagcg cgacgtgatc caggcccgcc actccggtga
     3241 ggtgggccag tgccgctcat cgatgccggc ccaccactgg cctgccgcct cggtccgcac
     3301 cagcgcgccg gcctggctca gttcgcccac ccattgcggg cgggtagcca gccagaagaa
     3361 gcccttggca cgcaggacac cgggccacga gccgcggatg aagccctgga aggcgtccgg
     3421 cacgaaaggc cgccgggccc gatacacgaa actgctgatg tcatattcct ccgtctccgg
     3481 catatggttc ttgaagccgt gaagctcctt gaaccaggcc gggtgatgat gcgcggtctc
     3541 gaagtcgaaa c
//
LOCUS       DBNGVR010000080         4172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2386051, whole genome shotgun sequence.
ACCESSION   DBNGVR010000080 DBNGVR010000000
VERSION     DBNGVR010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2386051"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="AC16A0_01775"
     CDS             <1..1302
                     /locus_tag="AC16A0_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104087.1"
                     /translation="AALAGIGLIVFFSFEERRITGYFLGGLVASFVLLLGLARLIIVA
                     AQRMPRSGSAIWRYAISNIYRPGSAAASVILALGLGLTLFVTLALTDRSISQELQSGL
                     PEKAPAFFFLDVRNAELEDFKAAIAKEAGVTGVSNAPMLRGRMVSVKGVPAEKVEATP
                     DSAWALRGDRGLTYSETLPKGSALTEGTWWPADYNGPPLVSLNDEIARGIGVNIGDEI
                     TVNVLGRDITAKVENLRQVNWRSLGINFVMVFSPNTLKSAPHSHIVTVEMEGGDEAAL
                     LNKMARAYPSVTAVRVKDALTIVSDLLNKMLTAIRGANVMTLLTGVLVLAGALAAGLS
                     ERLYDAVVLKTYGASRRQLIGAFVIEYAALGLAAALFGLLVGSLGSWFLARFILEMPW
                     SFSFATAALTALIAMAVTVAAGLAVTWQALSARPAPFLRNE"
     gene            1359..1943
                     /locus_tag="AC16A0_01780"
     CDS             1359..1943
                     /locus_tag="AC16A0_01780"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3104088.1"
                     /translation="MVVLNRIYTRTGDAGETALGNGERVSKASLRIAAYGTVDETNAV
                     LGIVRLHTAGADMAKLDAMLARIQNELFDLGADLCVPDTGKDLGYEPLRILPKQYERI
                     ETEIDDLNGELQPLRSFVLPGGHPAAAHLHLARTICRRAERLIVELAGKEGEHVSEGA
                     IAYINRLSDFFFVASRWANAKATGDVLWVPGKTR"
     gene            1943..2635
                     /locus_tag="AC16A0_01785"
     CDS             1943..2635
                     /locus_tag="AC16A0_01785"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3104089.1"
                     /translation="MFVPLHDDTPLKVIRFQHVTIFIIAINMLVYLVTGAFTSENALM
                     AYASGFGLVPGELTGSVAPYMGYDPVPEPLTLITYQFLHAGWMHLISNMLFLWVFADN
                     VEDAYGHVAFVMLYLLSGVIGGLAHVLFTPGSPEPLIGASGAVSGIIGAYVVLYPKAR
                     VWILLFMRIPIRIGAAWVLGGWFLLQLISLFAAREDGVSVAWLAHIGGFLTGFGLTWA
                     IRRRLLIQIGDS"
     gene            2740..3486
                     /locus_tag="AC16A0_01790"
     CDS             2740..3486
                     /locus_tag="AC16A0_01790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3104090.1"
                     /translation="MKVLVAVKRVVDYNVKIRVKADGSGVELANVKMSMNPFDEIGVE
                     EAVRLKEAGKATEVVAVSIGPAQAQETLRTALAMGADRAILVKHDAYVEPLGVAKILK
                     GVVEAEKPDIVILGKQAIDDDCNQTGQMLAALLGWAQATACSKLEPGAGEATITREID
                     GGLQTLKVKMPLIVTTDLRLNQPRYASLPNIMKAKKKPLDEKTPADFGADVAPRHKVV
                     KTEEPPKRQAGVKVKSPAELVAKLKEAGAI"
     gene            3489..>4172
                     /locus_tag="AC16A0_01795"
     CDS             3489..>4172
                     /locus_tag="AC16A0_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3104091.1"
                     /translation="MAVLLIAEHDKASLKDATHKAHSAALKMGGDVHVLVAGNKAGAA
                     AAQAAKLAGVKKVLHVESPALERPLAEPMAALLVSLAPAYDHLVAAATTNGKNYMPRV
                     AALLDVMQISDISDVVSGDTFVRLIYAGNAVQTVQTSDAKKVITVRTASFPAAAEGGS
                     APVETVNAAADPGLSSYVSENLTQSDRPELTSAKVIVSGGRGMQNGDNFKLLDSVARK
                     LNAAVGASRA"
BASE COUNT          716 a         1428 c         1309 g          719 t
ORIGIN      
        1 gccgccctcg ccggcatcgg actgatcgtc ttcttcagct tcgaggagcg gcgcatcacc
       61 ggctatttcc tcggcggcct ggtggccagc ttcgtgctgc tgctgggcct cgcccggctc
      121 atcatcgtcg cggcgcagcg catgccgcgc tccggatcgg cgatctggcg ctatgccatc
      181 agcaatatct atcggcccgg ttcggccgcg gcctcggtga tcctcgcgct cggacttggg
      241 ctcaccctct tcgtgacgct ggcgctcacc gaccgcagca tcagccagga actgcagtcc
      301 ggcctgcccg agaaggcgcc agccttcttc ttcctcgacg tgcgcaacgc cgagctggaa
      361 gacttcaagg cggcaatcgc gaaggaagcc ggcgtcaccg gcgtcagcaa tgcccccatg
      421 ctgcgcggcc gcatggtcag cgtgaagggc gtgccggccg agaaggtgga ggccacgccc
      481 gattccgcct gggcgctgcg cggcgaccgc gggctcacct attccgaaac gctgccgaag
      541 ggctccgccc tcaccgaagg cacatggtgg ccggccgact acaacggccc gccgctggtc
      601 tcgctcaacg acgagatcgc gcgtggtatc ggcgtcaata tcggcgacga gatcaccgtg
      661 aacgtgctgg gccgcgacat caccgcgaag gtcgagaacc tccgccaggt gaactggcgc
      721 agcctcggca tcaacttcgt catggtcttc tcgcccaata cattgaagtc ggcaccgcac
      781 agccacatcg tcacggtgga gatggagggc ggtgacgaag cggccctgct caacaagatg
      841 gcgcgggcct atccatcggt aacggcggtg cgcgtcaagg atgcgctcac catcgtgagc
      901 gacctgttga acaagatgct gaccgccatc cgcggcgcca atgtcatgac ccttctgacc
      961 ggcgtgctgg tgctggccgg cgcgctggcg gcgggcctct cggaacggct ctacgatgcc
     1021 gtggtgctca agacctacgg cgcctcgcgc cgccagctca tcggcgcctt tgtcatcgaa
     1081 tatgcggcgc tgggcctggc tgccgcgctc ttcggcctgc tggtgggatc gctcggctcc
     1141 tggttcctgg cgcgcttcat cctggagatg ccctggagct tctccttcgc caccgccgcc
     1201 ctgaccgcgc tcatcgccat ggctgtgacg gtggcagccg ggctcgcggt cacctggcag
     1261 gcactctcgg cccggcccgc gcccttcctc aggaacgagt aattgccttc tcccgccgaa
     1321 ggtgggacaa ggcggaagca caggcgaggc agcgcaacat ggtcgttctc aaccgcatct
     1381 acacccgcac cggcgatgcc ggcgagaccg cgctgggcaa cggcgagcgc gtctccaagg
     1441 ccagcctccg cattgccgcc tatggcacgg tggacgagac caatgcggtg ctcggcatcg
     1501 tgcgcctgca caccgcgggc gcggacatgg cgaagctcga tgccatgctg gcgcgcatcc
     1561 agaacgaact cttcgacctc ggcgccgacc tctgcgtgcc ggataccggc aaggatctcg
     1621 gctacgaacc attgcgcatc ctgcccaagc aatacgaacg catcgaaacc gagatcgacg
     1681 acctcaacgg cgaactgcag ccgctgcgct ccttcgtgct gcccggcggg catccggcag
     1741 ccgcgcatct gcatcttgcg cgcaccatct gccgccgcgc cgaacgcctg atcgtggaac
     1801 tcgccggcaa ggagggcgaa catgtgagcg aaggcgccat cgcttatatc aaccgcctgt
     1861 cggacttctt cttcgtggcc agccgctggg ccaatgcgaa ggccaccggc gacgtgctgt
     1921 gggttcccgg caagacgcgc tgatgttcgt cccgctccac gacgatacgc cgctgaaggt
     1981 catccgcttc cagcatgtga ccatcttcat catcgcgatc aacatgctgg tctatctcgt
     2041 gaccggcgcc ttcacttccg agaatgcgct gatggcctat gcctcgggct tcgggctggt
     2101 accgggagag ttgacgggaa gtgttgcccc ctacatgggc tacgacccgg tgccggaacc
     2161 gctgaccctg atcacttacc aatttctcca tgccggctgg atgcatctca tcagcaacat
     2221 gttgttcctg tgggtcttcg cggacaatgt ggaggacgcc tacggccacg tggccttcgt
     2281 gatgctctac ctgttgagcg gggtcatcgg cgggttggcc catgtgctgt tcacgcccgg
     2341 ttcgccggaa ccgctgatcg gtgcttcggg agcggtgtcc ggcatcatcg gcgcctatgt
     2401 ggtgctctac cccaaggcca gggtctggat cctgctcttc atgcgtattc cgatccgcat
     2461 cggagcggcc tgggtgctgg gcggctggtt cctgctgcag ctgatcagcc tctttgccgc
     2521 ccgggaggac ggcgtgagcg ttgcctggct ggcccatatt ggtgggttcc tgaccggttt
     2581 cggcctgacc tgggcaatcc gccgccgcct gctgatccag atcggcgact cgtgacgtga
     2641 agtcgcaatc cgccagcaag cgccgttgat ggaattgact tgagggattc ccctcactag
     2701 ggtccggcca ctttttgcgc cgccaaatcg aggaagatga tgaaagtcct ggtcgctgtg
     2761 aagcgggtcg tcgactacaa cgtcaagatc cgtgtgaagg ccgacggttc gggtgtcgaa
     2821 ctggccaatg tgaagatgtc gatgaatccc ttcgacgaaa tcggcgtcga ggaagccgtg
     2881 cgcctgaagg aagccggcaa ggcgaccgag gtggtggccg tctccatcgg accggcccag
     2941 gctcaggaaa cgctgcgcac cgcgctggcc atgggtgccg accgcgccat cctggtcaag
     3001 catgacgcct atgtcgaacc gcttggcgtc gccaagatcc tgaagggcgt ggtcgaggcc
     3061 gagaagcccg acatcgtcat cctcggcaag caggcgatcg acgacgactg caaccagacc
     3121 ggccagatgc tggccgcact cctcggctgg gcccaggcca ccgcctgctc caagctggaa
     3181 cccggcgccg gcgaagccac catcacccgc gagatcgatg gcggcctgca gaccctcaag
     3241 gtcaagatgc cgctgatcgt caccaccgac cttcgcctca accagccgcg ctatgcttcg
     3301 ctccccaaca tcatgaaggc gaagaagaag ccgctggacg agaagacccc ggccgacttc
     3361 ggcgccgatg tcgcgccgcg ccacaaggtg gtgaagacag aggagccgcc gaagcgccag
     3421 gccggcgtca aggtgaagtc gccagccgaa ctcgtcgcca aactcaagga agcgggagca
     3481 atctgatcat tgccgttctc ctcatcgccg aacacgataa agcctcgctg aaggacgcca
     3541 cccacaaggc ccactcggcc gccctcaaga tgggcggcga cgtgcatgtg ctggtggccg
     3601 gcaacaaggc cggcgccgcc gccgcccagg ccgccaaact ggccggcgtc aagaaggtgc
     3661 tgcacgtgga gtcgcctgcg ctcgagcgcc cgctggccga gccgatggca gccctcctcg
     3721 tgagcctcgc gcccgcctat gaccacctcg tggccgccgc cactaccaac ggcaagaact
     3781 acatgccgcg ggtggccgcg ctgctcgacg tgatgcagat ctccgacatt tccgacgtgg
     3841 tctcgggcga cactttcgtg cgcctgatct atgccggcaa cgcggtgcag acggtgcaga
     3901 cctcggatgc caagaaggtg atcacggtgc gcaccgcctc cttcccggct gccgccgagg
     3961 gcggctccgc gcccgtggag acggtcaatg ccgccgccga tccgggcctc tcgtcctatg
     4021 tcagcgagaa cctgacccag tcggaccgcc ccgaactcac ttcggccaag gtcatcgtct
     4081 cgggcggccg cggcatgcag aacggcgaca acttcaagct gctcgattcg gtggcgcgca
     4141 agctcaatgc cgccgtcggc gcctcgcgcg cc
//
LOCUS       DBNGVR010000081         7676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_619709, whole genome shotgun sequence.
ACCESSION   DBNGVR010000081 DBNGVR010000000
VERSION     DBNGVR010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_619709"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1491
                     /locus_tag="AC16A0_01800"
     CDS             34..1491
                     /locus_tag="AC16A0_01800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3104092.1"
                     /translation="MTETCDFLIIGGGSSGCIVAKRLAERTHGRIILLEAGKSDEGDP
                     AATDLNRLDEQTADYDWGFRAATLPGRPAELGYARARLLGGCANHNDCAFIRPPDSDF
                     DLWESLGATGWNAQALAPAYTRILERVTVETAPCHPLSRAFLDAGRELGLPDVDFARE
                     VRAGVGLFPLNARGRLRQSSSIAYLHPLDRLPPHLEVRTRSMAQRILCNGREAVGVET
                     DQGIILARRGVILACGAIQTPQLMMVSGFGPAAELRRQGIPVIAELPHLGQHLRDHVA
                     APVVWETRAPVAPWEICPFEATMMLSLDTEAPAPDILFHFGLRVREKYGDDPRLGTKG
                     PAVKASPNVTRARSEGEIRLSGPAMADKPIINLNYFSDPYDLPLLLRAMRFTRRIGET
                     AAMRAVTAAEVHPGPEVQSDDEWSAYVRSVCETVYHPCGTAAIGKVVSPGLGVMGIDR
                     LHVVDASVFPSLITVNINSAVMMVAERAADLIAQS"
     gene            complement(1482..3149)
                     /locus_tag="AC16A0_01805"
     CDS             complement(1482..3149)
                     /locus_tag="AC16A0_01805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR3104093.1"
                     /translation="MNAKKKFDHSNDLVFLPLGGTGEIGMNCYCYGVGPADDRSWLMV
                     DLGVKFGEDTEPGIDVVLPDVGFIASDRRNLAGIVLTHAHEDHLGAVAWLWPQLQCPV
                     YCSPFAAQILALKLKEAGLDEEVPVKVQALGSTFKVGPFDLEFVSVTHSIPEPSALLI
                     KTKQGTVLHSGDWKIDRSPVLGRGMDEARLRQIGDAGVDVLVCDSTNVLREGHSPSEA
                     DVAATIERIVAEAKGRVAITTFASHVDRLATSVRAARAVGREVVVVGRAMRNTIEAAR
                     ACGYLGDAGRFLDEEEFGYLPPDKILLLCTGSQGEPRAAIARIAEDQHPHVSLEPGDL
                     VIFSSRTIPGNEKEVSRIQNNLARLDIDVITADDALVHSSGHPRQGELRELYAWLRPA
                     ALIPMHGEPRHLRAHANFARDCGIRDVLIPRDGHIFKLAPGPLRDVDEAPFGRLHVDG
                     RLIVPVDDGPARQRRKLSFVGAVFVGVVLNAKQDLAADIDLITDGLPMGLEDDLREAA
                     ENAIGSSPKPRRRDRNQLAETIRVSVRRAADMAWGKKPVVKVSITQL"
     gene            complement(3146..3892)
                     /locus_tag="AC16A0_01810"
     CDS             complement(3146..3892)
                     /locus_tag="AC16A0_01810"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3104094.1"
                     /translation="MRSAAGHPVLHFERIDSTNIEARRLAEQGERGPLWLVADQQDGG
                     RGRLGRTWVSEPGNLYATLLFPMSAGPEIAAQAGLVAAVAVQEAVSRLRPDLAVRIKW
                     PNDLLIGGAKFCGILSEVVGTSPTRIALGCGINVAHAPKDTPYPATALGLRCTVESVF
                     QELDSSLSNCLKIWDEGRGFEAIRAAWTAAALGIGGQVTASIAGEATSGIFAGIAPDG
                     ALLLQTGDGKLRPIHSGEVRFAALEELRQA"
     gene            complement(3889..5337)
                     /gene="nuoN"
                     /locus_tag="AC16A0_01815"
     CDS             complement(3889..5337)
                     /gene="nuoN"
                     /locus_tag="AC16A0_01815"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HIR3104095.1"
                     /translation="MTLLQHADAVAALPEIVLAVLAMALLMAGVFRKSDSADAITLGA
                     IVSLALVAALVIWGGEGRQTAFGGAFVVDTFARVMKVLALFGSAVAIVLSVQFMKRDR
                     SERFEYPVLILLSAVGMMTMISANNLIALYMGLELQSLALYVVAAINRDSARASEAGL
                     KYFVLGALSSGMLLYGASLVYGFTGTTAFPQIAEVLSHGGEHGTSLGLIFGLVFILAG
                     LAFKVSAVPFHMWTPDVYEGAPTPVAAFFAAAPKVAAMALFIRVVVSPFGAIVPDWQQ
                     ILIFMAIASMALGAFAAIGQTNIKRLMAYSSIANMGFALVGLAAGTPEGVQGVIVYMM
                     IYMIMTLGTFACIIAMRRKGLQVEDINDLAGLSRSNKGLAFVFSMLMFSLAGIPPLAG
                     FFAKYFVFLAAMKAGLYVMAIIGVISSVIGAYYYLRVVKIIYFDEPAEAFDRMDPEVK
                     LVVYAAGAFTLLFVIFANPLIDLAAQAAQSLY"
     gene            complement(5347..6861)
                     /locus_tag="AC16A0_01820"
     CDS             complement(5347..6861)
                     /locus_tag="AC16A0_01820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR3104096.1"
                     /translation="MMSWPILSVVTFLPLLGVAFILALRGDDETAIRQTRLIALWTTV
                     FTFLLSLLIWINFDAAAPGFQFVEKHAWLGGAASYQMGVDGISMPFVILTTFLMPICI
                     LASWHVTKRVREYMIAFLVLETLMVGVFCALDLVLFYLFFEAGLIPMFLIIGIWGGPR
                     RVYASFKFFLYTLLGSVLMLLAIIYMYWTAGTTDIVALLANPVFTPEAQKWLWLAFFA
                     SFAVKMPMWPVHTWLPDAHVEAPTAGSVILAAILLKMGGYGFLRFSLPMFPDASLYFQ
                     DFVFVLSVIAIVYTSLVAMMQEDMKKLIAYSSVAHMGFVTMGIFSMNRQGIDGAMFQM
                     ISHGLVSGALFLCVGVVYDRMHTREIAAYGGLVKTMPLFAFTFMVFTMANVGLPGTSG
                     FVGEFLSLLGAFQANTWVAFFATTGVILSAAYALWLYRRVVFGKLEKPALMHRPDMTP
                     RELVTLAPLVVLTIFFGAYPAPILDIFGASVETLIKPLQQAAAAAEALATASLQ"
     gene            complement(6858..>7676)
                     /locus_tag="AC16A0_01825"
     CDS             complement(6858..>7676)
                     /locus_tag="AC16A0_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3104097.1"
                     /translation="MRKMGGLAPHIRITWAMMIIGNLALTGFPFTAGYFSKDAVIESA
                     YAANWGIAFALLIVSAFFTSFYSWRLAFITFNGKPRASAEVMSHVHESPNVMLIPLYV
                     LAVGALAAGFVFKDYFIGHHYAEFWHGAVADNEIMHLFHEVPAWVVWSPFVAMVLGFG
                     LAWLFYIVNTSLPQKLAAQHQPLYRFLLNKWYFDELYDFIFVRPAMWLGRFLWKKGDG
                     FVIDGFGPDGVASTVSDVTRGVVRLQTGYLYHYAFVMMIGVAILISWFAFGGAH"
BASE COUNT         1502 a         2453 c         2525 g         1196 t
ORIGIN      
        1 cgggcttcac tatgatcgca gtgtggcgtg cgcatgaccg aaacgtgtga ctttctcatc
       61 atcggcggcg gctcatccgg atgcatcgtc gcgaagcgcc tcgcggagcg gacgcacggc
      121 cggatcattc tcctggaagc cggaaagtcg gatgagggcg acccggcggc caccgatctc
      181 aaccgccttg acgagcaaac tgccgactac gactggggtt tcagagccgc gacccttccc
      241 ggccggcccg ccgaacttgg ctatgcgcgc gccaggcttc tgggtggctg cgccaatcac
      301 aatgactgcg cattcatccg tccgcccgac tccgacttcg acctgtggga atcgcttggg
      361 gcgacgggct ggaacgcgca ggctcttgcc cccgcctaca cgcgcatcct cgaaagggtc
      421 accgtcgaga ccgcgccctg ccatccgctg agccgcgcct tcctggacgc cggacgcgaa
      481 ctcggcctgc ccgacgtgga cttcgccagg gaggtacgcg ccggggttgg cctctttccg
      541 ctcaatgcgc gcggccgcct gcgccagtcc tcctccattg cctatctgca tccgcttgac
      601 cgactgccgc cgcatctgga ggtgagaacg cggagcatgg cgcagcgcat cctctgcaat
      661 ggccgggagg ccgtgggcgt cgagaccgat caaggcatca ttctcgcccg ccgcggcgtc
      721 attctcgcct gcggcgcaat ccagactccg cagctgatga tggtctcggg cttcgggccg
      781 gcggccgaat tgcgccgaca aggcataccg gtcatcgcgg aattgccgca cctcggacag
      841 catctgcgcg accacgtcgc cgcacccgtg gtctgggaaa cccgtgcgcc ggtggcgccg
      901 tgggagattt gccccttcga ggcgaccatg atgctgtcgc tcgacaccga agccccggcc
      961 cccgacatcc tgttccattt cggcctcagg gtgcgcgaga agtatggtga cgacccccgg
     1021 cttggaacca aaggccctgc cgtcaaggcc tcgcccaatg tgacgcgggc ccgcagcgag
     1081 ggcgagatcc gcttgtccgg tcccgcgatg gcggacaagc cgatcatcaa tctcaactac
     1141 ttctcggacc cctatgacct gccgctgctg ctgcgcgcca tgcgctttac tcgccgcatc
     1201 ggcgagacgg cggcgatgcg cgccgtgacg gcggccgaag tccatccggg gccggaggtg
     1261 cagagcgatg acgagtggtc agcctatgtc cgttcggtct gcgagacggt ctatcacccc
     1321 tgcggcacgg cggcgatcgg caaggtggtc agtccaggtc tcggcgtcat gggcatcgac
     1381 aggctgcatg tcgtcgatgc ctcggtcttt ccctcgctga tcacagtcaa catcaacagc
     1441 gccgtcatga tggtggcgga acgcgccgcc gatctcatcg ctcagagctg agtgatggac
     1501 accttcacca ccggcttctt gccccaggcc atgtcggccg cgcgcctcac ggagacacgg
     1561 attgtttccg ccagctgatt gcgatccctg cggcgcggct tcggactgga gccgatggcg
     1621 ttctcggctg cttcgcgcag atcgtcctcg agccccattg gcaggccgtc ggtgatgagg
     1681 tcgatgtcgg cggcaagatc ctgcttggcg ttcagcacca cgcccacgaa gaccgcaccg
     1741 acgaagctca gcttccggcg ctgccgggcc gggccatcgt ccaccggtac gatcagccgg
     1801 ccatcgacat gcaaacggcc gaagggcgcc tcgtcgacat cgcgcagcgg gccgggcgca
     1861 agcttgaaga tgtgaccgtc ccgcgggatc aggacatcgc gaatgccgca gtcgcgggcg
     1921 aaattggcat gggcgcgcag gtggcgcggc tcgccgtgca tggggatcag cgccgctggt
     1981 ctcagccagg catagagttc gcgcagctcg ccctggcgcg gatggccgga ggaatgcacc
     2041 aacgcgtcgt ccgcggtgat cacgtcgatg tcgagccgtg caaggttgtt ctggatacgg
     2101 gacacttcct tttcgttgcc ggggatggtg cgcgaggaga agatcacgag gtcgccaggt
     2161 tccagcgaca cgtgcggatg ctggtcctcg gcaatgcgcg cgatggcggc acgcggctcg
     2221 ccctggctgc cggtgcagag cagcaggatc ttgtcgggcg gcaggtagcc gaactcctct
     2281 tcgtcgagga accggccggc gtcgccgagg tacccgcagg cacgcgccgc ctcgatggtg
     2341 ttgcgcatgg cgcggccaac cacgaccacc tcgcggccaa cggcccgtgc ggcgcgcacc
     2401 gaagttgcga gccggtcgac atgcgaggcg aatgtggtga tggcaacgcg gcccttcgct
     2461 tcggccacga tgcgttcgat ggtcgccgcc acgtcggcct cggaggggga atggccctcg
     2521 cgcagcacat tcgtcgaatc gcagacgagg acatcgacgc ccgcatcgcc gatctggcgc
     2581 agccgcgctt cgtccatgcc acggcccagc accggactgc ggtcgatctt ccagtcgccg
     2641 gaatgcagca ccgtgccctg cttggtcttg atcagcaggg ccgagggctc ggggatggaa
     2701 tgtgtgaccg acacgaactc gagatcgaaa ggccccacct tgaacgtcga acccagtgcc
     2761 tgaaccttta ccggcacctc ctcgtccagg cccgcttcct tgagcttcag cgcaaggatc
     2821 tgcgccgcga aaggcgaaca atagaccggg cactgcagct gcggccacag ccaggcgacg
     2881 gcacccagat ggtcttcatg tgcgtgggtg agcacgatgc cggcaaggtt gcgcctgtcg
     2941 ctggcgatga agcccacgtc cggcagcacc acgtcgatgc ctggctcggt gtcctcgccg
     3001 aacttcacgc ccaggtcgac catcagccag ctgcggtcat cggcggggcc aacgccatag
     3061 caatagcagt tcatgccgat ctcgccggtg ccgcccagcg gcaggaagac gaggtcgttg
     3121 ctgtggtcga acttcttctt cgcgttcatg cctgtctcaa ctcttcgagc gcggcgaaac
     3181 ggacctcacc ggaatggatc ggccgcagct ttccgtcgcc ggtctgcagc agcagggcac
     3241 catccggtgc gatgccggcg aagatgccgg atgtcgcttc gccggcgatg ctggccgtaa
     3301 cctgcccgcc aatccccagg gcggccgcgg tccacgccgc gcggatcgcc tcgaaacctc
     3361 gcccctcatc ccagattttc aagcagtttg aaagacttga atcaagttcc tgaaaaaccg
     3421 attcaaccgt gcagcgcagc cccagggcgg tggccggata gggcgtatcc ttcggcgcat
     3481 gagcgacgtt tatgccgcag ccaagcgcga tccgggtggg cgacgtgccc acgacctccg
     3541 aaaggatgcc gcagaacttg gcgccgccga tcagcaggtc gttgggccac ttgatgcgga
     3601 cggccagatc cggccgcagc ctgctgacgg cctcctgcac cgccacggcc gcaaccagac
     3661 ccgcctgcgc ggcgatctcc ggcccggcgg acatgggaaa gaggagcgtc gcatagagat
     3721 ttccgggctc cgagacccag gtccggccca ggcggccgcg gccgccgtcc tgctggtcag
     3781 ccaccagcca gagcggaccg cgctcgccct gctccgccag ccgccgggcc tcgatgttgg
     3841 tcgaatcgat ccgctcgaaa tgcaggaccg gatgtccggc cgccgacctc aatagagcga
     3901 ctgcgcggcc tgggccgcga ggtcgatcag cggattggcg aagatcacga agaggagggt
     3961 gaaggcgcct gccgcataga cgaccagctt cacctcggga tccatcctgt cgaaggcttc
     4021 ggcaggctcg tcgaaataga tgatcttcac cacccgtagg taataatagg cgccaatgac
     4081 cgacgagatg acgccgatga tcgccatcac gtagaggccg gccttcattg ccgcgaggaa
     4141 gacgaagtac ttggcaaaga aaccggccag cggcggaatg ccggccagcg agaacatcag
     4201 catggagaag acgaaggcca ggcccttgtt gctgcgcgac agtccggcga ggtcgttgat
     4261 gtcctcaacc tgcagaccct tgcggcgcat ggcgatgatg caggcgaagg tgcccagcgt
     4321 catgatcatg tagatcatca tgtagacgat cacgccctgc acgccttccg gcgttccggc
     4381 cgcgagcccc accagcgcga agcccatgtt ggcgatcgag gaataggcca tcaggcgctt
     4441 gatgttggtc tggccgatgg cagcaaaggc gccgagcgcc atggaggcga tggccatgaa
     4501 gatcaggatc tgctgccagt ccggcacgat ggcgccgaag ggcgacacca ccacacggat
     4561 gaagagcgcc atcgcggcca cctttggcgc ggcggcgaag aaggcggcaa ctggcgtcgg
     4621 cgcgccttca taaacgtcgg gcgtccacat gtggaagggc acggcggaga ccttgaaggc
     4681 cagtccggcc aggatgaaga cgaggccgaa gatcagtccc aggctggtgc cgtgttcgcc
     4741 gccatgggac aggacttccg cgatctgcgg gaaggccgtc gtgccggtga agccgtagac
     4801 cagcgaggcg ccgtaaagca gcatgccgga ggacagcgcg cccagcacga aatacttgag
     4861 acccgcttcc gatgcccggg cgctgtcgcg gttgatggcc gccaccacat agagcgccag
     4921 cgactgcagc tcgaggccca tgtagagcgc gatcaggttg ttggccgaga tcatggtcat
     4981 catgccgacg gccgagagca ggatgaggac cgggtactcg aaacgttccg aacggtcgcg
     5041 cttcatgaac tgcaccgaca gcacgatagc gaccgccgaa ccgaagagcg ccagcacctt
     5101 catcacgcgg gcgaaggtgt cgaccacgaa ggcaccgcca aaggcggtct gccgcccctc
     5161 cccgccccag atcaccaggg ctgccaccag cgccagcgac acgatggcgc ccagcgtgat
     5221 ggcgtcggcg gagtcgctct tgcggaagac cccggccatg agcagcgcca tggccagcac
     5281 cgccagaacg atctccggca gggccgcgac ggcatcggca tgttgaagaa gcgtcatggc
     5341 ggatccttac tgcaacgaag ccgtggccag cgcttccgcc gcggcggccg cctgctgcaa
     5401 tggcttgatg agggtttcga cggaggcccc gaagatatcg agaatgggcg cgggataggc
     5461 gccgaagaag atggtgagca ccaccagcgg cgccagcgtc accagttcgc gcggagtcat
     5521 gtccggccga tgcatcagcg ccggcttttc cagcttgccg aagaccacgc ggcggtagag
     5581 ccagagcgca taggctgccg agaggatgac gcccgtggtg gcgaagaagg ccacccaggt
     5641 attggcctgg aatgcaccga gcagcgacag gaattcgccc acgaaaccgg aggtgcccgg
     5701 caggcccaca ttggccatcg tgaagaccat gaaggtgaag gcaaagagcg gcatggtctt
     5761 caccagtcca ccataagcgg cgatctcgcg ggtgtgcatg cggtcataga ccacgccgac
     5821 acagaggaag agcgcgcccg acaccagccc gtggctgatc atctggaaca tggcgccgtc
     5881 gatgccctgg cggttcatgg agaagatgcc catggtcacg aagcccatgt gcgccaccga
     5941 ggaatatgcg atcagctttt tcatgtcctc ctgcatcatc gccaccagcg aggtgtagac
     6001 gatggcgatg accgagagca cgaagacgaa gtcctggaaa tagaggctgg catccggaaa
     6061 catgggcagc gagaaacgca ggaagccata gccgcccatt ttcaggagga tggcggccag
     6121 gatcaccgaa cccgcggtcg gggcttccac atgcgcgtcg ggaagccagg tgtgcaccgg
     6181 ccacatcggc atcttcaccg cgaaggaggc gaagaaggcc agccatagcc atttctgcgc
     6241 ctcgggcgtg aagacggggt tggcgagcag tgcgacgatg tcggtcgtgc cggccgtcca
     6301 gtacatgtag atgatggcca gcagcatcag caccgagccg agcagcgtgt agaggaagaa
     6361 cttgaaactg gcatagacgc gacgcggccc gccccagatg ccgatgatca ggaacatcgg
     6421 gatcaggccc gcctcgaaga agaggtagaa gagcaccagg tcgagcgcgc agaacacgcc
     6481 gaccatcagc gtctccagca ccaggaaggc gatcatgtat tcgcgcaccc gcttggtgac
     6541 gtgccaggac gcgagaatgc agatcggcat caggaaggtg gtgaggatga cgaagggcat
     6601 ggagatgccg tcgacgccca tctggtagga ggccgcacca ccgagccagg catgcttctc
     6661 gacaaactgg aagccgggcg ccgctgcatc gaagttgatc cagatcagca gcgacagcag
     6721 gaaggtgaag accgtggtcc acagtgcgat cagccgcgtc tggcggatgg cggtttcgtc
     6781 atcgccgcgc agcgccagga tgaaggccac gcccagaagc ggcaggaagg tgacgaccga
     6841 gagaatgggc caactcatca gtgtgcgccc ccgaatgcga accaggaaat caggatggcc
     6901 acgccgatca tcatgacgaa ggcgtagtga tagaggtagc cggtctgcag acgcaccacg
     6961 ccgcgggtca cgtcgctcac cgtcgaggcc acgccatcgg gcccgaagcc gtcgatgacg
     7021 aagccgtcgc ccttcttcca caggaatcgg cccagccaca tggccggacg cacgaagatg
     7081 aagtcataaa gctcgtcgaa gtaccacttg ttgagcagga agcggtagag cggctggtgc
     7141 tgggccgcga gcttctgcgg caggctggtg ttgacgatgt agaagagcca ggcgaggccg
     7201 aagcccagga ccatggccac gaagggcgac cagaccaccc aggccggcac ctcgtggaag
     7261 agatgcatga tctcgttgtc ggccactgcg ccatgccaga actcggcata gtgatggccg
     7321 atgaaatagt ctttgaacac gaagccggcg gccagcgcgc cgacggccag cacatagagc
     7381 gggatcagca tcacgttggg actttcgtgc acgtggctca tcacctccgc gctggcccgc
     7441 ggtttgccgt tgaaggtgat gaaggcgagt cgccaggaat agaagctggt gaagaaggcc
     7501 gagacgatca gcagcgcgaa ggcgatgccc cagttggcgg cataggcgct ctcgatgacg
     7561 gcgtccttcg agaagtagcc ggcggtgaag gggaatcccg tcagcgccag gttgccgatg
     7621 atcatcatgg cccaggtgat gcggatgtgg ggcgcgaggc cgcccatctt gcgcat
//
LOCUS       DBNGVR010000082         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3959426, whole genome shotgun sequence.
ACCESSION   DBNGVR010000082 DBNGVR010000000
VERSION     DBNGVR010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3959426"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..788)
                     /gene="rpsD"
                     /locus_tag="AC16A0_01830"
     CDS             complement(171..788)
                     /gene="rpsD"
                     /locus_tag="AC16A0_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3104098.1"
                     /translation="MTKRLTAKHKLDRRMGENIWGRPKSPVNKREYGPGQHGQRRKGK
                     LSDFGVQLKAKQKLKGYYANITERQFRAIYAEASRMTGDSSENLIGLLERRIDAVVYR
                     SKFGPTMFAARQFVSHGHVKLNGRRVTIPSIRLKAGDTIELTPKAKDLAVVLEATQLA
                     ERDVPDYVEANHSNMTARLVRVPGLSDVPYPVKMEPNFVVEYYSR"
     gene            1002..1835
                     /locus_tag="AC16A0_01835"
     CDS             1002..1835
                     /locus_tag="AC16A0_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3104099.1"
                     /translation="MQPSLSLRLLRDGLAVAVVAAFLFPLFWWGLTSIKPGYAILDQH
                     RIVVFDFNPTWDSYAVTLAGAGPASFDSRASLANTAIVALGSTAITLAAALPLGFAMS
                     VFTFRFRRSIFYWVLFQRFLPPIAIIFPLVMTYHAIGLRDTLLGVALAHAALNLPFAA
                     LLLKSFYDDVPPEVSEAAVIDGASRLQIFARIATPMIRGGIAATAVLCMIFSWTEFLM
                     SLFLTLEIRMFPVQASIVVTNMWGYTSALSTAAMLPAFAFILLVQRHLVRGLTLGMTK
                     G"
     gene            complement(1832..2644)
                     /gene="murI"
                     /locus_tag="AC16A0_01840"
     CDS             complement(1832..2644)
                     /gene="murI"
                     /locus_tag="AC16A0_01840"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR3104100.1"
                     /translation="MTAPRILIFDSGMGGLTVARAVARQLPDAQLVYAADNAGFPYGA
                     WKEEDLVARILDVIGALIEKVEPHVVVIACNTASTIALAQLREAYKLPFVGTVPAIKP
                     AAAQTKSGIIGVLATPGTVSREYTHALIHTYAYHCKVFLHGAKRLAEIAENKLKGRSV
                     DLDELRREIAPVFRKREGRSTDVVVLGCTHYPLLVEEIAQVAPWPVTYLDPAPAIARR
                     VADVIDETPRPEALGVVPAHGTVFLTSPRGGASDALGAYAAMGFPLPVFMER"
BASE COUNT          437 a          913 c          859 g          524 t
ORIGIN      
        1 acgacgtgcc aggcttccat cccatcctcc ccacccgtcc cgaaacggga caccccgttc
       61 atgatttcga aacgaagcaa gaagggtgcc ggaaaccgcc tggccggctc cggcacagaa
      121 aagccgccgg cggtgaagcc ggcggctcgc aagagggcgc tgggccggac ttagcgcgaa
      181 taatactcga cgacgaagtt cggttccatt ttcaccggat agggaacgtc cgagagtccc
      241 ggaacgcgca ccagccgggc ggtcatgttg gagtggttgg cctcgacata atcgggcacg
      301 tcacgttcgg ccagctgggt ggcttccagc accacagcaa ggtcttttgc cttgggcgta
      361 agctcaatgg tgtcgccggc cttgaggcgg atcgagggga tggtcacgcg gcggccgttc
      421 agcttcacgt ggccatggct cacgaactgg cgggcggcaa acatggtggg accgaacttg
      481 gagcgataga ccaccgcgtc gatgcggcgc tccagcagac cgatgaggtt ctccgaggag
      541 tcgccggtca tgcgcgaggc ttcggcatag atggcgcgga actggcgttc cgtgatgttg
      601 gcgtagtaac ccttgagctt ctgcttggcc ttcagctgca cgccgaagtc ggacagcttg
      661 cccttgcggc gctggccatg ctggcccggg ccatattcac gcttgttgac ggggctcttg
      721 gggcggcccc agatgttctc gcccatgcgg cggtcgagtt tgtgcttggc ggtcagacgc
      781 ttcgtcattt acggtctcca tgttgtgtgg acccaccctc ctcgacaccg gtttcccggc
      841 cgacagcgcc agtgatggcg cgcgggcaag caaacacggg gcgcttcggc cccgtgtctg
      901 gcggcgcgta taggggaagc gcctgtagct gtcaaatccc gggacagctg aacgatcgac
      961 ccgagcttgc gggcggccgt ggcgtccagc taccctgcct catgcaacca agcctctctt
     1021 tgcgcctgct ccgcgacgga ctggccgtgg ccgtcgtggc tgccttcctc ttcccgctct
     1081 tctggtgggg cctgacgtcg atcaagccgg gctacgccat cctggaccag caccggatcg
     1141 tcgtcttcga cttcaatccc acctgggaca gttatgccgt caccctggcc ggggcggggc
     1201 cggcctcctt cgactcccgc gccagcctgg cgaacaccgc gatcgtcgcc ctgggttcaa
     1261 ccgcgatcac cttggccgca gccctgcccc ttggattcgc catgtccgtc ttcaccttcc
     1321 gcttccggcg cagcatcttc tactgggtcc tgttccagcg cttcctgccg cccatcgcga
     1381 tcatcttccc cctggtcatg acctatcatg cgatcgggtt acgcgacacg ctgcttgggg
     1441 tggcgcttgc ccatgccgcc ctcaacctgc ccttcgccgc cctgctgctc aagtcgttct
     1501 acgacgatgt gccgccggag gtgagcgagg ctgcagtgat cgacggcgcg agccgtctgc
     1561 agatcttcgc ccgtatcgca accccgatga ttcgcggcgg catcgccgca acggccgtgc
     1621 tctgcatgat cttctcctgg acggaattcc tgatgtcgct cttcctgacc ttggagatcc
     1681 gcatgttccc ggtgcaggcc agcattgttg tcaccaacat gtggggctac acctcggccc
     1741 tcagcacggc ggcgatgctg ccggccttcg cgttcatcct gctggtgcag cgccatctgg
     1801 tccgcgggct gaccctgggc atgacaaagg gctagcgttc catgaacaca ggcagcggga
     1861 aacccatggc tgcataagcc cccaatgcgt ctgatgcccc gccgcgcggc gatgtcagaa
     1921 acactgttcc atgcgcaggg actacgccca gcgcctccgg ccgcggcgtc tcgtcgatca
     1981 cgtcggccac gcgccgggcg atggcgggcg ccggatcgag ataggttacg ggccagggtg
     2041 cgacctgggc gatctcctcg acaagcagcg ggtaatgcgt gcatcccagc accaccacgt
     2101 cagtgctccg gccttccctc ttgcggaaaa cgggtgcgat ttcgcggcga agttcatcga
     2161 gatcgacgct gcggcctttc aacttgttct cggcgatctc ggccagccgc ttcgcgccat
     2221 gcaggaagac cttgcagtga taggcatagg tatggatcag cgcatgggta tactcgcggc
     2281 tcaccgtccc cggggttgcc agcacgccga tgattccgct tttcgtctgg gccgccgccg
     2341 gcttgatcgc cggcacggtg ccgacaaagg gcaacttgta ggcttcccgc agctgtgcca
     2401 gcgcaatggt ggaggcggtg ttgcaggcaa tcaccaccac atgcggctcc accttctcga
     2461 tcagcgcgcc gatgacgtcg agaatgcggg caaccaggtc ctcttccttc catgcgccgt
     2521 agggaaaccc cgcattatcc gcggcataga cgagctgtgc gtcgggcagc tgccgcgcca
     2581 cggcgcgcgc cacggtcagt ccgcccatgc cggaatcgaa aatcaggatg cggggggcgg
     2641 tcatgatcgc gggcccttgc gccgctcatg ggcgcgcgtc agcgaggcga ccatgccgcg
     2701 gagcgagcgc acttcctgct cggtgaggcc ggc
//
LOCUS       DBNGVR010000083         7726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4154418, whole genome shotgun sequence.
ACCESSION   DBNGVR010000083 DBNGVR010000000
VERSION     DBNGVR010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4154418"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC16A0_01845"
     CDS             complement(<1..454)
                     /locus_tag="AC16A0_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3104101.1"
                     /translation="MTLEPAEALLLRSWRASTRIAALPETVRPVNRAGGYEVAARLAI
                     LTGDRVAGWKIAATSTAGQKHINVDGPLIGRIFEKRLLAAGAEVQLGDNIMKVAEAEF
                     AFAFARDLPPRGEDYSLDEAMQAVAALHLSIEVPDSRYADFTRVGAPSL"
     gene            complement(462..1625)
                     /locus_tag="AC16A0_01850"
     CDS             complement(462..1625)
                     /locus_tag="AC16A0_01850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3104102.1"
                     /translation="MSFELPPDARAFRDKVKSFVDSELRPLEVEAEMNGGRISDELRK
                     KHERMAIALGLSRMDVPRDRGGPGLSVLAQVAATEAIGSVTNALGWCYAEAQNWMFEA
                     CNDDQMARYVMPLTTGDIHLCYAITEENAGSDPAAIEATALREGDHYVINGEKWHVTS
                     HNHANTIVVQAKLESGDHCLFFCPADAPGISIKRSPAYTHNYDHHHPILTFKDVRVPL
                     RDRIGDEGDGMSFTHAWFRRERLMIAARCCGAMERLIEEATGFAKTRVVSGEPIANYQ
                     FIQGMLADSVVDLQAARLVTYEAAAAHDRGEDVKSLHARCSIAKLLASEAAGRVADRA
                     VQIFGGRGYMRENVAERFYRELRVDRIWEGTSEIQRLIIARSLLKRGLAATIG"
     gene            complement(1629..3479)
                     /locus_tag="AC16A0_01855"
     CDS             complement(1629..3479)
                     /locus_tag="AC16A0_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3104103.1"
                     /translation="MERRLTTVLSADVAGYSRLMGVDEDGTLGELKTRHDLYVAPLLA
                     RHGGRLIKQMGDGFLAEFASVVNAVHFAVDIQAAADQRNAEVPEALQMRMRVGINLSD
                     VIADQNDVYGDGVNVAARLQALSEPGGVCISETVYQHVNRHLSYNFEDLGKQRLRNIS
                     GAVQVYRLRPNAGGAPGARRKSGEGHEASIVVLPFANMSGDPEQEYFSDGITEDIITD
                     LSKISSLFVVSRNTAFTFKGQHVDIDLVATRLGVQYVLEGSIRRSGDRVRITAQLIDG
                     RTNGHVWAERYDRDMKDFFALQDDISRSIVAALRIRMLPSEAATLTHKVRGTPEAYRY
                     YLMARGFFHRGHTKRYLRIAKHIFEKARDLDPGYAEAYAGIADCNSHLLDAGDSSISV
                     DDIMEQSARALALNPQLAEAHASKGLALYTAGRYEEAESCFITAIGLKPDLFEAYLFY
                     GRNCLNRGRYEQAADLFGMAAELKNDDFRALGLQAMCYQSLNRMEDAQAAALAALARA
                     EKAVATRPDDADALAFGAGLLAVLGETDRTKDWAERAVIIEPDDHYMHYNLACAFAIL
                     GEVELALDRLAQALGPRSVTSLKEYMLNDSDLDVLRDHPRYAAILKSLSE"
     gene            3650..4489
                     /locus_tag="AC16A0_01860"
     CDS             3650..4489
                     /locus_tag="AC16A0_01860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3104104.1"
                     /translation="MSFAQPRSLEEAVSLLSGGAATLLAGGTDLYPAHVGRPLPRHVV
                     SLGRVTELRGISRHGAGFRLGGGTTWSEIAAANLPPAFDALRQAARDVGSIQIQNRGT
                     IAGNLCNASPAADGVPPLLALDAAVELASANGTRQLPLSEFITGYRRTARQPDEILSA
                     VIVPPCAGRSAFVKLGARRYLVISIVMAGACVEMAPDGSIASVRVAIGAASPVAQRLP
                     QLERELAGLAAGIRPSSRVATRHVESLRPIDDVRAEAWYRRDAAIHVLGAALDRAAGF
                     ESS"
     gene            4486..7224
                     /locus_tag="AC16A0_01865"
     CDS             4486..7224
                     /locus_tag="AC16A0_01865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3104105.1"
                     /translation="MSGVSFRLNGRNVVSASPAVTRLSNVLRDEFRATGTKSGCDAGD
                     CGACTVQIDGELACACLVPVAEAEGRSVVTVEGLAANTSTGQALQDSFLAQGAAQCGI
                     CTPGMLVAAARLLDHERAPSRARIADAMSGVLCRCTGYSKIIDAIVGVASAPCTVATP
                     KAGKAVGARIARLDGHPKVAGQEAYGADAIPPDALFVRAIRSPYHRARFSFGDLDAFL
                     AASPGIIRVLTAVDIPGRNIFGVIPPFADQPVFATGETRFRGEAVAAIVGECSGISDF
                     DEGSFPIVWTEKPAVLTPEQAEDPSAALIHENRPGNILVRGRVIRGDTDTAIAGAAAV
                     VEGEFSTGFVEHAYIEPEAGWARRVKDRIEVTVSTQSPYLDRDDVAAILGIEPDAVRI
                     IPSACGGGFGSKLDLSVQPFVAIAAWLLGRPAAMVYTRPESIVSTTKRHPARMRARMA
                     ADADGRLVAMEFDGAFNTGAYASWGPTVANRVPVHASGPYVVPNYRAESRAVLTNCVP
                     AGAFRGFGVPQTVVAQEQMLDMLALKLGLDPLDFRIANALTDTTPTVTGQVMGDGVGI
                     RACFEALVPHWQRARRDAVAFNQGQGRKRRGAGVAGMFYGCGNTSLPNPSTIRIGLKP
                     DGRIALHQGAVDIGQGSNTVIAQIAADALGLPLACFDLVAPDTDLTPDCGKTSASRQT
                     FVTGKAAELSGRALRRAMLRLANAGEDATLDVADGRLIVIEDAVVRAVILERQPRDAH
                     GYVLCAEESFDPPTAPLDPDGQGIPYAVYGYGAHLAEVEVDLALGTVKVLKITAAHDV
                     GRAINPVLVEGQIEGGVAQGIGMALMEEFTPGRGENLHDYLIPTIGDVPPIESILIEC
                     ASAVGPFGAKGIGEQALIPTAPAILNAINHATGARITRTPATPDRVRAAIKAMERP"
     gene            7221..>7726
                     /locus_tag="AC16A0_01870"
     CDS             7221..>7726
                     /locus_tag="AC16A0_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HIR3104106.1"
                     /translation="MTEAQQDKIRCDACPVLCYIREGLRGACDRYANEGGQLVRVDPH
                     ILIERTVKEGGRLVPFLDRGKDWDGSIVAYPGTFVTAIGAGTTYPDYKPAPFIVSSEI
                     DGVDMITVVTEGIFSYCGVKVKIDTDRHLGPETATVRVNGEAVGHVTTAEYGSQMLSL
                     GGVRHLTGG"
BASE COUNT         1226 a         2596 c         2547 g         1357 t
ORIGIN      
        1 tgagcgacgg cgcaccgacc ctggtgaagt cagcgtagcg gctgtccggc acctcgatgg
       61 agagatgcag tgccgccaca gcctgcatgg cttcgtccag gctgtagtcc tcgccgcgcg
      121 gcggcaggtc gcgcgcgaag gcgaaggcaa actccgcctc ggccaccttc atgatgttgt
      181 cgccgagctg gacctccgcc ccggccgcca gtagccgctt ctcgaagatg cgtccgatca
      241 gaggcccgtc cacgttgatg tgcttctggc cggcggtgct ggtggcggcg atcttccacc
      301 cggcaacccg gtcgccggtc agtatggcga ggcgtgcggc aacctcatag ccaccggcgc
      361 ggttcaccgg ccgaacggtc tcgggcaggg cagcgatccg cgtcgaggcc ctccagctcc
      421 tgagcaacag cgcttcggcc ggttcgagag tcatggctgc ctcacccgat ggtcgctgcg
      481 aggccgcgct tcagcagcga tcgcgcgatg atcagacgct ggatctcgct ggtgccttcc
      541 cagatgcggt ccacgcgcag ttcccggtag aagcgctcgg ccacattctc gcgcatgtag
      601 ccgcggccgc cgaagatctg caccgcgcgg tcggccacgc ggcccgcggc ctcggaggcc
      661 aggagcttgg cgatggagca gcgggcgtga agcgatttca catcttcgcc gcgatcatgc
      721 gccgccgcag cttcgtaggt gaccaggcgc gccgcctgca ggtcgacgac agagtcggcc
      781 agcatgccct ggatgaactg gtaattggcg atcggctcgc ccgagaccac acgcgtcttg
      841 gcaaaccctg tcgcctcctc gatgaggcgc tccatggcgc cgcagcagcg cgcggcgatc
      901 atcagccgct cgcggcggaa ccaggcatgg gtgaagctca tgccgtcgcc ctcgtcgccg
      961 atgcggtcgc gcagcggcac gcgaacatcc ttgaaggtga ggatcggatg atggtggtcg
     1021 tagttgtgtg tgtaggcggg gctgcgcttg atgctgatgc ccggcgcatc ggccgggcag
     1081 aagaagaggc agtgatcgcc gctctcgagc ttggcctgga ccacgatggt attggcatga
     1141 ttgtggctgg tcacgtgcca cttctcgccg ttgatcacgt agtgatcgcc ttcgcgcagg
     1201 gcggttgcct cgatcgcggc gggatcggac ccggcgttct cctcggtaat ggcgtagcag
     1261 agatgaatgt cgcccgtggt cagcggcatc acgtaacgcg ccatctggtc gtcgttgcag
     1321 gcctcgaaca tccaattctg cgcttcggcg tagcaccatc caagcgcatt ggtgacgctg
     1381 ccgatcgcct cggtcgccgc aacctgcgcc agcacggaga ggccaggtcc gccccggtcc
     1441 cggggcacgt ccatgcgcga caggcccagc gcgatcgcca tgcgttcatg cttcttccga
     1501 agctcgtcgg agatgcggcc gccgttcatt tcggcctcga cttccagcgg gcgaagctcg
     1561 ctgtcgacga atgacttgac cttgtcgcgg aaggcgcgtg catcgggggg cagctcgaaa
     1621 ctcatgggtt actccgacag ggacttgaga atggcggcat atcgcggatg gtcgcgcagg
     1681 acatccagat ctgaatcgtt cagcatgtat tccttgagag atgtgaccga acgcggcccg
     1741 agcgcctgcg ccagccggtc tagcgcaagt tccacttcgc cgagaatggc gaaggcgcaa
     1801 gccagattgt agtgcatgta atggtcatcg ggctcgatga tcacggcgcg ctcggcccag
     1861 tccttggtcc ggtccgtctc gcccagcacg gcaaggagtc cggccccgaa ggccagcgca
     1921 tcggcatcgt ccggccgggt ggcgacggcc ttctcggccc gcgccagcgc ggcaagggcg
     1981 gcggcctgcg cgtcttccat gcgattcagc gactggtagc acatggcttg caggccaagc
     2041 gcccggaagt cgtcattctt cagttcggcg gccatgccga agaggtcggc ggcctgctcg
     2101 tatcgcccgc ggttcaggca gttccggcca tagaacagat aggcttcgaa gagatccggc
     2161 ttgaggccga tagcagtgat gaagcagctt tcggcctctt cgtatcgtcc ggccgtatag
     2221 agcgccaggc ccttggaggc atgggcttcg gccagttgcg gattgagtgc aagggccctt
     2281 gcgctctgct ccatgatgtc gtcgaccgaa atgctgctgt cgcctgcatc gagcagatgc
     2341 gaattacagt cggcgatgcc ggcataggcc tccgcatagc ccggatcgag gtcgcgcgcc
     2401 ttctcgaaga tatgcttggc gatgcgcagg taacgcttgg tgtggccgcg gtggaagaaa
     2461 ccgcgcgcca tgagatagta gcgataggcc tccggcgtgc cgcgcacctt gtgggtgagc
     2521 gtggcggcct ccgagggcag catgcggatc cgcagcgcgg cgacgatgct gcgcgagatg
     2581 tcgtcctgca gcgcgaagaa gtccttcatg tcgcggtcgt agcgctcggc ccagacatgg
     2641 ccgttggtgc ggccgtcgat gagctgcgcg gtgatcctga cccggtcgcc cgacctgcgg
     2701 atgctgcctt cgagcacgta ctgcacgcca agccgggtgg ccacgagatc gatgtcgacg
     2761 tgctggccct tgaaggtgaa ggccgtgttg cgcgacacca cgaagagcga cgagatcttg
     2821 gacaggtcgg tgatgatatc ctcggtgata ccatcgctga agtattcctg ctccggatcg
     2881 ccgctcatgt tcgcgaaagg caggaccacg atcgaggcct cgtgtccttc gccgctcttg
     2941 cgccgggcac ccggtgcgcc gccggcattg ggccgaaggc ggtagacctg cacggccccg
     3001 gagatgttgc gcaggcgctg ctttccgaga tcctcgaagt tatagctcag gtgccggttc
     3061 acatgctggt agaccgtctc ggaaatgcac acgccgccgg gctcggaaag ggcctgcagg
     3121 cgagccgcca cattcacgcc atcgccgtag acatcgttct gatcggcgat cacgtcggac
     3181 agattaatgc cgacccgcat gcgcatctgc agcgcttcgg ggacctcggc attccgttga
     3241 tcggcggccg cctggatatc gaccgcgaaa tgcacggcgt tcacgacgct ggcaaactcc
     3301 gccaggaatc cgtcgcccat ctgcttgatg aggcgtccgc catggcgggc aagcagaggc
     3361 gcgacataga gatcgtgccg tgtcttgagc tcgcccagcg ttccgtcttc gtcaaccccc
     3421 atcagccggg aatagccggc gacgtcggcg gacagaaccg ttgtcaggcg ccgttccact
     3481 ccatgaatct cctcgagaag ggacctagaa taggccccgc tcgcgccctt agcaatttga
     3541 ttcggcaccg gattccccta cctccgggtg cgccgcgctt gacacctgcg aggccgcggg
     3601 ggagactcgt catgggccgc gcggtgcggc cagccatgtg gtcctgccga tgtcatttgc
     3661 acagccccgt agcctggagg aagccgtgtc gcttttgtct ggcggtgccg ccacgcttct
     3721 tgccggcgga accgatctct atccggcgca tgtcggccgg cccttgccac ggcacgtcgt
     3781 gtcgctcggg cgggtgaccg agctgcgcgg gatcagccgg catggcgcag gcttccgcct
     3841 cgggggcggc acgacctgga gcgagatcgc cgcggccaac ctgccgcccg ccttcgacgc
     3901 gctccgccag gccgcgcgtg acgtcggatc gatccagatc cagaaccgcg gcaccatagc
     3961 cggcaatctc tgcaatgcct cgcccgcggc cgacggcgta ccgccgctgc tggcgctcga
     4021 tgccgcagtc gaactggcct cggcaaatgg aacgcgccag ttgcccctgt cggagttcat
     4081 caccggctac cggcgcaccg cccggcaacc tgacgagatt ctttccgccg tcatcgtccc
     4141 gccctgtgcc ggccgttcgg ccttcgtcaa gctgggcgcc cggcgctacc tggtgatctc
     4201 catcgtgatg gccggggcct gcgtggagat ggcaccggat gggtcgatcg cttcggtgcg
     4261 cgttgccatc ggcgccgcct cgccggtggc gcaacgcctg ccgcagcttg aacgcgagct
     4321 tgccggactt gccgccggca tccggccgtc ctcccgtgtc gcgacccggc atgtcgagtc
     4381 gctcaggcca atcgacgacg tgcgtgccga ggcctggtac cggcgggatg cggcaatcca
     4441 tgtcctgggt gcggctctgg accgggccgc gggattcgaa tcctcatgag cggcgtttcc
     4501 tttcggttga atggcaggaa cgtcgtcagt gcatcgccgg cggttacccg gctcagcaat
     4561 gtgctgcgcg acgagtttcg cgccaccggc accaagtcgg gctgcgacgc gggcgactgc
     4621 ggtgcctgca cggtgcagat cgatggcgag ctcgcctgcg cctgcctcgt tccggttgcg
     4681 gaggccgagg gcagatccgt cgtgacggtc gagggactgg cggcgaacac ctccacgggg
     4741 caggcactgc aggactcgtt cctcgcgcag ggcgccgccc aatgcggcat ctgcacaccg
     4801 ggcatgctgg tggcggccgc ccgccttctc gatcatgaga gagcgccatc gcgcgcgcgc
     4861 attgccgatg caatgtccgg cgtcctctgc cgctgtacgg gctattccaa gatcatcgat
     4921 gcgattgtgg gcgtggccag cgcgccgtgc acggtcgcca cgccgaaggc aggcaaggcg
     4981 gtgggcgcgc gcatcgcgcg cctcgatggg catcccaagg ttgcgggtca ggaggcctat
     5041 ggcgccgacg ccattccgcc ggacgcgctc tttgttcggg ccatccgttc gccctatcac
     5101 cgcgcccgct tcagcttcgg cgaccttgac gccttcttgg ccgccagtcc cggcatcatc
     5161 cgtgtgctga cggctgtgga cattcccggc cgcaacatct tcggcgtgat tccgcctttc
     5221 gccgatcagc cggtcttcgc caccggcgaa acgcgcttcc gcggcgaggc cgtggcggcc
     5281 atcgtcggcg aatgcagcgg catctcggac ttcgacgagg gcagctttcc gatcgtctgg
     5341 accgaaaagc cggccgtcct caccccggag caagcggaag atccttcggc cgctttgatc
     5401 cacgagaacc gacccggcaa tatcctggtg cgcggccgtg tgatccgcgg cgacacggac
     5461 acagccatcg caggggccgc ggcggtggtg gaaggcgagt tctcgaccgg gttcgtcgag
     5521 cacgcctata tcgaacccga ggcgggctgg gcccggcgcg tgaaggaccg catcgaggtg
     5581 accgtctcca cgcaatcgcc ctatctggac cgcgacgatg tcgccgccat tctcggaatc
     5641 gagcctgatg ccgtgcggat cattccttcc gcctgcggcg gcggcttcgg ctcgaagctc
     5701 gatctctcgg tgcagccctt cgtggcgatc gcggcctggc ttctcggacg cccggcggcc
     5761 atggtctata cgcgtccgga atccatcgtc tccaccacca agcgccatcc cgcccgcatg
     5821 cgcgcgcgca tggctgcgga tgctgacggc aggctcgtgg cgatggagtt cgatggcgcc
     5881 ttcaatacag gcgcctatgc gtcatgggga ccgaccgtcg ccaaccgcgt gccggttcac
     5941 gcgtcgggtc cctatgtcgt tcccaactac cgcgccgaga gccgcgcggt tctgacgaat
     6001 tgcgtgcctg ccggcgcctt ccgcggattc ggcgtgccgc agaccgtcgt tgcccaggag
     6061 cagatgctgg acatgctggc gctcaaactg ggtctcgacc cgctggactt ccgcatcgcc
     6121 aatgcgctca ccgatacgac accaaccgtc accggccagg tcatgggtga cggcgtcggc
     6181 attcgtgcct gcttcgaggc gctggtgccg cattggcagc gggcgcgccg cgacgcggtg
     6241 gccttcaacc agggccaggg gcgcaagcgc cgtggcgcag gcgtcgcggg catgttctat
     6301 ggatgcggca atacctcgct gcccaatcca tccaccattc gcatcggcct gaagccggat
     6361 ggccgcatcg cgctgcacca gggtgccgtg gacatcggcc agggctccaa caccgtcatc
     6421 gcacagatcg ccgccgatgc gctgggcctg ccgcttgcct gtttcgatct ggtcgcgccc
     6481 gacaccgatc tcacgcccga ctgcggcaag acatccgcat cgcgccagac ctttgtcacc
     6541 ggaaaagcgg ctgaactgtc gggacgtgcg cttcgccgcg caatgctgcg cctcgccaac
     6601 gccggcgaag acgcgacgct cgatgtcgcc gatggccggc tcatcgtgat agaggatgcg
     6661 gtggtgcgcg cggtcatcct cgaacggcag ccgcgcgacg cgcatggcta cgtgctctgc
     6721 gcggaagaga gcttcgaccc gccgactgcg cccctcgatc cggatgggca gggcattccc
     6781 tacgccgtct atggctatgg cgctcacctc gccgaggtgg aggtcgatct cgctctcggc
     6841 accgtgaagg tcctcaagat cacggcagca catgatgtcg gccgtgccat caatcctgtc
     6901 ctggtcgaag gccagatcga aggcggcgtc gcccagggca tcggcatggc gctgatggag
     6961 gagttcacgc ccggacgcgg cgagaacctg cacgactatc tcattcccac catcggcgac
     7021 gtgccgccca tcgagtccat cctcatcgag tgcgcctcgg ccgtcggccc cttcggcgcc
     7081 aagggcattg gcgaacaggc gctgatcccg accgccccgg cgatcctcaa tgccatcaac
     7141 catgccactg gcgcacgcat cacccgcacg ccggccacac cggatcgcgt gcgggccgcc
     7201 atcaaggcga tggagcgccc atgacggaag cgcaacagga caagatccgc tgcgacgcct
     7261 gcccggtgct ctgctacatc cgggaggggc tccgtggcgc ctgcgaccgc tacgccaacg
     7321 agggcggcca gctggtgcgc gtcgatccgc acatcctgat cgaacgcacg gtgaaggagg
     7381 gcggccggct ggtgcccttc cttgaccgtg gcaaggactg ggacggcagc atcgtcgctt
     7441 accctggcac cttcgtcacg gccattgggg ccggcacgac ctatcccgac tacaagcccg
     7501 cgcccttcat cgtatcgtcc gagatcgacg gtgtcgacat gatcaccgtc gtcaccgaag
     7561 gcatcttcag ctactgcggc gtgaaggtga agatcgacac cgaccgccat cttggccccg
     7621 agaccgccac cgtgcgcgtc aacggcgagg cggtcggcca tgtgacgacg gcggagtacg
     7681 gctcgcagat gctttcgctc ggtggcgtca ggcatttgac cggcgg
//
LOCUS       DBNGVR010000084         3356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5137087, whole genome shotgun sequence.
ACCESSION   DBNGVR010000084 DBNGVR010000000
VERSION     DBNGVR010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5137087"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="AC16A0_01875"
     CDS             <1..588
                     /locus_tag="AC16A0_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3104107.1"
                     /translation="LLDGIGDAELAIIVDPVDGTSNFAWGLPLFGVMASVIRKGVTVA
                     GLIYDPVGRDWRKALKGEGAWAENASGRTHDLKVAKPAPVSEMTGLCSWYIMPEPLRS
                     RTVANLSKTKASFSYRCAAYEYRMVAEGLCHFSLHYKLMPWDHAAGVLIHGEAGGFSA
                     LLDGRPYSATIHQGGVMSAPDRDSWTLLQQELLAG"
     gene            585..1448
                     /locus_tag="AC16A0_01880"
     CDS             585..1448
                     /locus_tag="AC16A0_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3104108.1"
                     /translation="MTTAGPIFDRALLARRLARIRGHEPNVLTRTIAEEAADRLDLVT
                     RRFATAAVIAADPDHIAERLMPSGKIDRVVPFAPAADEVLGLEAESFDAIFSILDLQT
                     ANDVPGLLVQMRRALRPDGLFLACLLAGDSLTELRQSWLAAEVLITGGVSPRVAPMIG
                     MQELGALLQRAGFALPVADLDRTIVRYADAVSLIHEIRALGYSNPLAGRSRKPVTRGL
                     LGAAVNAYHANFADADGRIRATLEVAWLTGWAPHESQQQPLKPGSAKARLADALRVPE
                     QKLRADRSDGE"
     gene            complement(1420..1818)
                     /locus_tag="AC16A0_01885"
     CDS             complement(1420..1818)
                     /locus_tag="AC16A0_01885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIR3104109.1"
                     /translation="MKIVLVAAVALVDADGRVLISKRPEGKQLGGLWEFPGGKVEAGE
                     RPEAALIRELKEELAIDVAESCLAPLTFASHAYDDFHLLMPLYVCRRWQGTVRGAEGQ
                     ELAWVRPLRLRDYAMPPADVPLIPHLIDLL"
     gene            complement(1818..3056)
                     /gene="argJ"
                     /locus_tag="AC16A0_01890"
     CDS             complement(1818..3056)
                     /gene="argJ"
                     /locus_tag="AC16A0_01890"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR3104110.1"
                     /translation="MGHQVSPLAPERFPELPPIRGVRLATGEAGIRYKNRPDVLLAVL
                     DPGTAVAGCFTKSKSRSAPVDWCVESLAAGSARAVVINAGNANAFTGKAGVATVKAVA
                     SAAARLLKCKPHDIFQASTGVIGEPLNPSPITAALPQLMEAARSDAWSEAARAIMTTD
                     TFAKACTVTAKLDGKTVTINGIAKGSGMIAPDMATMLVFIFTEAAIPSRVLQKLLSRS
                     VGSSFNCITVDGDTSTSDTVLMFATGAAGKPAKALASAADARLKGFAKALDSLCLDLA
                     LQVARDGEGAEKLIEIAVTGAENDKAAHRIAMSIANSPLVKTAIAGEDANWGRVVMAI
                     GKAGEKAIRDKLRITIGGVTVARNGMRDPAYREAEIMPHMKGRHVVITADVGVGKGKA
                     RVWTCDLTHRYIDINGSYRS"
     gene            complement(3090..>3356)
                     /locus_tag="AC16A0_01895"
     CDS             complement(3090..>3356)
                     /locus_tag="AC16A0_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104111.1"
                     /translation="AQPFDQVKSAIRNVLLRKKVADTMEKIRAASKVEIVDEDLKKYA
                     EDAAKAAKTFQDKQAGQAGQTVVPAAEGGDAPATTGKGDLQLQQ"
BASE COUNT          545 a         1146 c         1067 g          598 t
ORIGIN      
        1 ctgctcgacg gcatcggcga cgcggaactc gccatcatcg tcgatcctgt cgacggcacc
       61 agcaactttg cctggggcct tccgctcttc ggcgtcatgg cttcggtgat ccggaagggc
      121 gttaccgttg cggggctgat ctacgatccc gtcggccgtg actggcgcaa ggccctgaag
      181 ggcgagggcg cctgggccga aaatgcatcg ggcaggaccc atgatctcaa agtcgcaaag
      241 cccgcaccgg tcagcgagat gaccggtctt tgctcgtggt acatcatgcc ggaacccctg
      301 cgatcccgca cggttgccaa tctgtccaag accaaggcca gtttcagcta ccgctgtgcg
      361 gcctatgagt accgcatggt tgccgaaggc ctctgccatt tctcgctgca ctacaagctg
      421 atgccctggg accatgccgc gggcgtgctg atccatggcg aagcgggagg cttctctgcg
      481 ctcctcgacg gcaggcccta tagcgccacc atccaccagg gcggggtcat gtcggcaccc
      541 gacagggaca gctggacact gctgcagcag gaactcctcg caggatgaca acggcagggc
      601 cgatctttga ccgcgccctg ctggcccggc ggctggcccg gatacgcggt cacgagccca
      661 atgttctgac ccgcaccatc gccgaggagg cggccgaccg cctggatctc gtgacccgcc
      721 gcttcgccac ggccgcggtc attgccgctg atcccgacca tatcgcggaa cggctgatgc
      781 cgtccggaaa gatcgacagg gtcgtgccgt tcgccccggc cgccgacgag gtacttggcc
      841 tcgaggcgga gtccttcgac gcgatcttca gcattctcga tctgcagacg gccaatgatg
      901 tcccgggcct cctggtccag atgcgccgcg ccctgcggcc cgacggactc ttcctcgcct
      961 gcctccttgc cggcgacagc ctgacggaac tccgccagtc gtggctcgct gccgaagtcc
     1021 tgatcactgg cggggtctcg ccgcgcgtgg cgccgatgat cggcatgcag gaactgggcg
     1081 ccctgctgca gcgggcgggc tttgcgctgc cggtcgcaga tctcgaccgc accatcgttc
     1141 gctatgccga tgcggtgtcg ttgatccatg agatccgggc cctgggctat tccaatcctc
     1201 ttgcgggccg gtcgcgcaaa cccgtcacgc gcggactctt gggggcggcc gtcaatgcct
     1261 atcatgccaa tttcgccgac gcggacggcc gcatccgcgc cacgctcgaa gtggcctggc
     1321 tcaccggctg ggcaccgcat gaaagccagc agcaacccct gaagcccggc agcgccaagg
     1381 cgcggctggc ggacgccctg cgcgtgccgg agcagaagct cagagcagat cgatcagatg
     1441 gggaatgagc ggaacatccg ccggcggcat cgcatagtcg cgcaggcgca gcggccgcac
     1501 ccaggccagt tcctgcccct ccgcgccgcg caccgtgccc tgccagcggc ggcagacata
     1561 gagcggcatc agcagatgaa agtcatcata ggcatggctg gcgaaggtga gcggcgcaag
     1621 acaggactcc gccacgtcga tggcgagttc ttccttcagt tctcggatca atgcggcttc
     1681 cggacgttcg cccgcttcca ccttgccgcc gggaaattcc cacaggccgc ccagttgctt
     1741 gccttccggc cgcttcgaga tcaggacgcg gccatcggca tcgaccagcg ccacggcggc
     1801 aaccaggacg atcttcatca gctgcggtag gagccgttga tgtcgatgta gcgatgggtc
     1861 aggtcgcagg tccagacccg cgccttgccc ttgcccacgc ccacgtctgc ggtaatcacc
     1921 acatgccgcc ccttcatgtg cggcatgatc tcggcctccc tgtaggccgg atcgcgcatg
     1981 ccattgcgcg ccacggtaac gccgccgatg gtgatgcgca gcttgtcgcg gatggccttc
     2041 tcgcccgcct tgccgatggc catgacgaca cggccccagt tcgcgtcctc gccggcaatg
     2101 gcggtcttca ccagaggcga attggcgatc gacatggcga tgcgatgggc cgccttgtcg
     2161 ttctcggcgc cggtgacagc gatctcgatc agcttttccg cgccttcgcc atccctggcc
     2221 acctgcagcg cgagatcgag gcacaagctg tcgagcgctt tggcgaagcc cttgagacgg
     2281 gcatccgctg ccgatgccag cgcctttgcc ggttttccgg ccgcaccggt tgcaaacatc
     2341 agcacggtgt ccgacgtcga ggtatcgcca tcgacagtga tgcagttgaa gctgctgcca
     2401 acgctgcgcg acagaagctt ctgcaggacc cttgaaggaa ttgccgcctc ggtgaagatg
     2461 aagaccagca tggtcgccat gtcgggcgca atcatgccag agcccttggc gatgccgttg
     2521 atggtgaccg tcttgccgtc gagcttcgcc gtgaccgtgc aggccttcgc gaaggtgtcg
     2581 gtggtcatga tcgcgcgggc ggcttcgctc catgcgtcag accgcgccgc ttccatcagc
     2641 tgcggaagcg cggcggtaat cggtgagggg ttgagcggct cgccaatcac gccggtggaa
     2701 gcctgaaaga tgtcgtgcgg cttgcacttc aggagccggg ccgcggcact cgccacggcc
     2761 ttgaccgtgg ccacgcccgc cttgccggta aaggcattgg cgttgccggc gttgatcacg
     2821 acagcccggg ccgagcccgc cgcgagactc tcgacgcacc aatcgaccgg ggccgagcgc
     2881 gacttggatt tggtaaagca tcccgccacc gctgtgcctg ggtccaggac cgccagcagc
     2941 acgtcgggac ggttcttgta gcggatgccc gcctcgccag ttgccagccg aacgccacga
     3001 atcggcggaa gctcgggaaa gcgctccggt gccagtggcg aaacctgatg tcccatcgac
     3061 agccccctcg ctagcccgga cgacgaaacc tattgctgga gctggagatc gcccttgccg
     3121 gtggtggccg gcgcatcacc gccctccgca gccgggacga cggtctgacc cgcctggccg
     3181 gcctgcttgt cctggaatgt cttggccgcc ttggcggcat cctcggcgta tttcttgaga
     3241 tcctcgtcga cgatctcgac cttcgaggcg gcgcggatct tctccatcgt gtccgccacc
     3301 ttcttgcgga gcagcacgtt gcggatcgcc gacttcacct ggtcgaaggg ctgggc
//
LOCUS       DBNGVR010000085         2693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4352124, whole genome shotgun sequence.
ACCESSION   DBNGVR010000085 DBNGVR010000000
VERSION     DBNGVR010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4352124"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="AC16A0_01900"
     CDS             <1..376
                     /locus_tag="AC16A0_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104112.1"
                     /translation="EALFRTDALGRMATQAIQSIRHTSMQQIDWFERVAVLDLRPPAW
                     IIRQEKLEEDFNTLLRRMGLPITCGDLKLATDSMAAHANDYGTAPDLSPGARDNLSRW
                     YARDFVFYDLCLDWMARQGGRP"
     gene            complement(380..589)
                     /locus_tag="AC16A0_01905"
     CDS             complement(380..589)
                     /locus_tag="AC16A0_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3329 domain-containing protein"
                     /protein_id="HIR3104113.1"
                     /translation="MPVPKEQGHPFYKPLWRRVVITAVVLFWFVFELINGGIGIWSFM
                     AFAMLVYAVYLFFLTWPKDEAGPES"
     gene            complement(589..1392)
                     /locus_tag="AC16A0_01910"
     CDS             complement(589..1392)
                     /locus_tag="AC16A0_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR3104114.1"
                     /translation="MRLSKTLAAAAISFVMCSAAAQAAKCGNTGAGFEAWKDAFADEA
                     RANGVGSKGLKALAGTSYSTATIKADRNQKSFKLSLDQFLEKRGASTIVSQGRKLKAA
                     NAKLFAQLEKRYGVPPGPLIAIWGMETAFGNYMGNQNTISAVATLAYDCRRPEFFTEQ
                     LYAALKLIDKGILSSGSKGAMHGEVGQTQFLPKNVLLYGADGDGGGINLNKKADALAS
                     TANFLRGHGWQRGAGYQPGETNFAALQGWNAASVYQRALAIMGEQIDGR"
     gene            complement(1533..1853)
                     /gene="trxA"
                     /locus_tag="AC16A0_01915"
     CDS             complement(1533..1853)
                     /gene="trxA"
                     /locus_tag="AC16A0_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3104115.1"
                     /translation="MSTHKVSDQSFEADVLKSDTPVVVDFWAEWCGPCKMIGPALEEI
                     AASLGPKAKIVKINIDENPSTPSKYGVRGIPTLMVFKGGQVAATKVGAAPKNQLQSWI
                     EQSI"
     gene            complement(1930..>2693)
                     /locus_tag="AC16A0_01920"
     CDS             complement(1930..>2693)
                     /locus_tag="AC16A0_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3104116.1"
                     /translation="GRQAVKSDCWYELAVQGLGPIMREVEDGIRRLGPDPVFGPARGS
                     PAAAAVDVPSWVRLSAPAEEDASPRTVSSLLEAAAAEAGLGHDAAARRGIVIHRILQL
                     LPDTAPDSRAALAQRLLARAGHAPEAAASVLKMLERPEMQDVFAPGGLSEIPLAVDLP
                     GLGKVVGRIDRLVVTPERILAVDFKTGQRVPAGPSDLDPMHVHQMAAYREALRRIHPG
                     TAIVLALLYTEAPKLLILDDDLLDRATHQIRVMRP"
BASE COUNT          464 a          858 c          907 g          464 t
ORIGIN      
        1 agaggcgctc tttcgcaccg acgcactggg gcgcatggcc acccaggcga ttcagtccat
       61 ccgtcacaca tccatgcagc agatcgactg gttcgagcgt gtggcggtcc tggacctgcg
      121 gccgccggca tggatcatcc ggcaggaaaa gctcgaggag gacttcaaca cgctgctcag
      181 gagaatggga ctgccgatca cctgcggcga cctgaagctg gcaacggaca gcatggctgc
      241 ccatgccaat gactatggaa cggcaccgga tctcagcccg ggcgccaggg acaatctgag
      301 ccggtggtat gcgcgggact tcgtcttcta cgatctctgc ttggactgga tggccaggca
      361 gggcggtcga ccctgaacct cagctctccg ggccggcctc atccttgggc caggtcagga
      421 agaagagata gaccgcatag accagcatgg cgaaggccat gaaggaccag atgccgatgc
      481 cgccgttgat cagttcgaag acgaaccaga acagcaccac cgcggtgatg acgacgcgcc
      541 gccacagcgg tttgtagaag ggatggccct gctccttcgg taccggcatc agcggccgtc
      601 gatctgttcg cccatgatgg cgagcgcgcg ctggtagacg ctggccgcgt tccagccctg
      661 gagcgcggcg aaattggttt cgcccggctg gtagccggcg ccgcgctgcc agccatggcc
      721 gcgcaggaag ttggcggtgg aggccagggc gtcggccttc ttgttgagat tgatgccgcc
      781 gccgtcgcca tccgcgccgt agagcagcac attcttcggc aggaactgcg tctggcccac
      841 ttcgccgtgc atggcgccct tgctgccgga cgacaggatg cccttgtcga tcagcttcag
      901 cgcggcatag agctgctcgg tgaagaactc gggccggcgg caatcatagg ccagcgtggc
      961 cacggccgaa atggtgttct ggttgcccat gtagttgccg aaggccgtct ccatgcccca
     1021 gatcgcgatc agcgggccgg gcggaacgcc atagcgcttt tcgagctggg cgaagagctt
     1081 ggcattggcc gccttgagct tgcgcccctg cgacacgatg gtggaggcgc cccgcttttc
     1141 caggaactgg tcgagcgaca gcttgaagct cttctggttg cggtcggcct tgatggtggc
     1201 ggtggaatag gaggttccag ccagcgcctt gaggcccttg gagcctacgc cattggcccg
     1261 cgcctcatcg gcaaaggcat ctttccaggc ctcgaagccc gcacccgtat tgccgcattt
     1321 ggcggcctgg gcagcggccg agcacatgac aaagctgatg gccgcggcgg ccaaagtctt
     1381 cgaaaggcgc atgttcttcc ccagcggtcg attcgactgg gtcaaaactg ccacaagctc
     1441 cgcgggttgt catcgattgc gatgcaagca ggcttaatga cgaagcccgg cctgaggggg
     1501 ccgggcttcg ggtcagaccg ggaatccggt tgtcagatcg actgctcgat ccagctctgc
     1561 agctggttct tgggcgccgc acccaccttg gtggcggcga cctggccgcc cttgaagacc
     1621 atcagcgtcg ggatgccgcg cacgccatac ttggagggcg tcgagggatt ctcgtcgatg
     1681 ttgatcttga cgatcttggc cttggggcca aggctggcgg cgatctcttc cagcgccggg
     1741 ccgatcatct tgcaggggcc gcaccactcg gcccagaaat ccaccaccac cggcgtatcg
     1801 gacttcagga cgtcggcctc gaaggactgg tcggacacct tgtgcgtgga catggaaacc
     1861 tcttggggat gcgggcggga ttcgcccctt tgcgtgatgt cgggaaggta ggagtggccc
     1921 tccccccggt caagggcgca tcacgcgaat ctgatgggtc gcgcggtcga gcagatcatc
     1981 gtccaggatc agtagcttgg gtgcctctgt ataaagcagt gccagcacga ttgcagtacc
     2041 gggatgtatg cggcgcaggg cttcgcgata ggcggccatc tgatgcacat gcatgggatc
     2101 gaggtccgac gggccagccg gtacccgctg cccggtcttg aaatccaccg ccaggatgcg
     2161 ctccggcgtg accaccagcc ggtcgatgcg gccgacaacc tttccgaggc cagggaggtc
     2221 cacggcgagc ggtatctccg acagcccgcc tggcgcgaag acgtcctgca tctccggccg
     2281 ctcaagcatc ttaagcacgg acgccgcggc ctccggcgcg tggccggccc gcgccaagag
     2341 gcgttgggcc agggcggcgc gcgagtccgg cgccgtgtcg ggcagaagct gcaggatgcg
     2401 gtggatgacg atgccgcggc gggcggcggc atcgtggccc aggcccgctt ctgccgcggc
     2461 cgcctcaagc aggctggaga ccgtacgcgg acttgcatct tcttcggccg gcgcggacag
     2521 tctcacccac gacggcacat cgaccgccgc agcggccggc gatcctcgcg ccggaccgaa
     2581 gaccggatcc gggccgaggc gccggatgcc gtcttcgacc tcgcgcatga tcggcccgag
     2641 gccctggacc gcaagctcgt accagcagtc ggacttgacg gcctgccggc cgg
//
LOCUS       DBNGVR010000086         1782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2781107, whole genome shotgun sequence.
ACCESSION   DBNGVR010000086 DBNGVR010000000
VERSION     DBNGVR010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2781107"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC16A0_01925"
     CDS             <1..222
                     /locus_tag="AC16A0_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016735563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR3104117.1"
                     /translation="GETPDEIFTIGNARPLDQALQHATTEMLEWLIAEHGHDAVSASH
                     LMGMAVRYDVANVFNPNYSVACRIAKAWL"
     gene            complement(233..1408)
                     /locus_tag="AC16A0_01930"
     CDS             complement(233..1408)
                     /locus_tag="AC16A0_01930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3104118.1"
                     /translation="MSAKIDDILAAARRHCDEVIRPGVDGWNAARNWPRAASDKAAAL
                     GLLGLYAPAEWGGQGLSLGDGIRVYEELGKGDGAYAFALSMHNICTFAVCGFGSEALK
                     RDWARDLTTGRKLANFSLTEPQSGSDASTMYSKARINGDGTWTINGAKAWVSLAGEAD
                     IYLTVVKTSDAPGHKDMAMIAIPRGIKGLSFGPLYDTPSYNFLPMGEMHLDNVVVPEA
                     NIILPVGKGLQGSLMAIDIARVSIAAGCCGLMQSALDTALGYTKNRKMFGGTALDLDG
                     IQWMLGEVATDLEASRLLYRAAANALGTPDGPLMAAHAKRFVPDAAIKAANICTQAMG
                     GMGLLTSYGLDRLSRLAQMLRIVDGTTEISRVVIGRALKKRAAELSDVPVPKGFGEA"
     gene            complement(1442..>1782)
                     /locus_tag="AC16A0_01935"
     CDS             complement(1442..>1782)
                     /locus_tag="AC16A0_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3104119.1"
                     /translation="TATSMIGFIALAGIIVRNSILLVDFIKHSWTPDRNLHDVLLEAG
                     AIRFKPILLTAIAAMIGAAVILTDPIFQGLAISLLFGLASSTALTVLVIPAIYLVLRG
                     GSLRREPVTG"
BASE COUNT          323 a          582 c          582 g          295 t
ORIGIN      
        1 ggcgagacgc cagacgagat cttcaccatc ggcaatgccc ggccgctgga ccaggccctg
       61 cagcacgcca ccacggagat gctggagtgg ctcatcgccg agcatggcca tgatgcggtg
      121 agcgccagcc atctcatggg catggccgtt cgctacgacg tcgccaatgt cttcaatccg
      181 aactactcgg tcgcctgccg cattgccaaa gcatggctct gacggatggg cctcaggcct
      241 cgccgaatcc cttgggcaca ggaacgtcgg aaagctccgc cgcgcgcttc ttcagcgccc
      301 gcccgatcac cacgcgggaa atctccgtcg ttccgtcgac gatccgcagc atctgcgcaa
      361 gcctggagag ccggtcgagc ccgtaggaag tgagcaggcc catgccgccc atcgcctgcg
      421 tgcagatgtt cgccgccttg atcgccgcat cgggcacgaa gcgcttggca tgagcggcca
      481 tcagtggacc atccggcgtg cccagtgcat tggcggccgc gcgatagagc agacgcgagg
      541 cctcgaggtc ggtcgccacc tcgcccagca tccactggat gccgtcgagg tccagcgcgg
      601 ttcccccaaa cattttgcga ttcttcgtgt agccgagagc cgtgtcgagg gccgactgca
      661 tcagtccgca gcagcccgcg gcgatcgaga cgcgcgcgat gtcgatggcc atgagcgatc
      721 cctgcaggcc cttgccgacg ggaagaatga tgttggcttc cggcacgacc acgttgtcga
      781 gatgcatctc gcccatcggc aggaaattgt aggacggcgt gtcatagagc ggcccgaagc
      841 tgaggccctt gatgcccctc ggaatggcga tcatcgccat gtccttgtgg cccggcgcat
      901 ccgatgtctt gaccacggtc aggtagatgt cggcctcgcc ggcgagtgac acccaggcct
      961 tggcgccgtt gatcgtccat gtgccatcgc cattgatgcg cgccttggaa tacatggtcg
     1021 acgcatccga acccgactgg ggttcggtca gcgagaagtt ggccagcttg cggcccgtgg
     1081 tgagatcgcg ggcccagtcc cgcttcagcg cctcgctgcc gaagccgcag accgcgaagg
     1141 tgcagatgtt gtgcatcgac agcgcgaagg cataggcgcc gtcgcccttg cccagctcct
     1201 cgtagacccg gatgccgtcg ccaagcgaga gcccctgccc gccccattca gccggcgcat
     1261 agagaccgag aagcccaagg gccgccgcct tgtccgacgc ggcgcgcggc cagttgcgcg
     1321 ccgcgttcca gccatcgacg cccgggcgga tgacctcgtc gcaatggcgg cgggcagccg
     1381 ccagaatgtc gtcgatcttg gcgctcatga gatcctctcg ctgtgcggaa tttagacgat
     1441 gctatcccgt gacgggctcg cgccgcaagc tgccgccgcg caggacgagg tagatcgccg
     1501 ggatgaccag cacggtgagc gccgtcgatg aggcgagacc gaagagcagc gagatcgcga
     1561 gaccctggaa gatcgggtcg gtcaggataa ccgcggcgcc gatcatggcc gcgatggcgg
     1621 tgagcaggat gggcttgaag cggatggcgc ccgcttccag cagaacgtca tggaggttcc
     1681 ggtccggcgt ccatgaatgc ttgatgaagt ccaccagcag aatcgagttg cgcacaatga
     1741 tgccggccag cgcgatgaag ccgatcatcg aggtggccgt ga
//
LOCUS       DBNGVR010000087         2650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3567139, whole genome shotgun sequence.
ACCESSION   DBNGVR010000087 DBNGVR010000000
VERSION     DBNGVR010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3567139"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16A0_01940"
     CDS             complement(<1..461)
                     /locus_tag="AC16A0_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104120.1"
                     /translation="MTSTQAPSIPPRADGLAIAAILFALFCVLAAFGATGLIRGALEH
                     LGAYSGAWQILFWTVIAAVALLFWPALRALKLARSANAAVAGGDIVTARIDGAASREL
                     GWITFGWGAFALIALGFFSFIMMNDAAVGKTFFYLPLMQEKWWQVTKQFFI"
     gene            complement(507..1349)
                     /locus_tag="AC16A0_01945"
     CDS             complement(507..1349)
                     /locus_tag="AC16A0_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104121.1"
                     /translation="MNITKRILLAAAAAIALAGPAAAADMIGNCELSGAKGSIPIEKP
                     AKAGQLTVEVSLPAPIWWNGDTPEAIKDGMEYCMAAEIAWRAGYDKLEVVNVGWDALI
                     AGQTADFDLAMSQISITEDRKKVHDFSVPYFNSDIGVLARTDAPVDEKTIKSAKVGVQ
                     QATTGAAFAQGTLGLTDVQVYPDQGDMFTALRAGQIDAAMTDTSIVLAEEVANPGKVA
                     VIGQYKTGETYGAIYPKGNPNNATLDKIIQSMIDDGTVAKLGAKYLAAAWGKDPATVP
                     YFNP"
     gene            complement(1456..>2650)
                     /locus_tag="AC16A0_01950"
     CDS             complement(1456..>2650)
                     /locus_tag="AC16A0_01950"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR3104122.1"
                     /translation="TAREACRIRVVPLFETIDDLRAGPAIMTELLKVPVVKGSIAANG
                     GRQEIMLGYSDSNKDGGFFCASFELFEAQRRLIRVGRDAGVEVSFFHGRGGSVSRGGA
                     PAGRAIAAQPAGTINGRMRVTEQGEVVSSKFANRGTAQHNLEVLAASVFVHTLKSIDE
                     AELKVVAEHQEAVEWIAGASFRHYRKLAEDPALLAYFHQASPVEELANLKLGSRPARR
                     FGAKGIGDLRAIPWVFAWSQNRHLLTGWYGLGYAFDDFLVPRGGEGLKLLREMYNRSR
                     GFRLAVDEVEKSLYLADMSVAEQYAELVEDRTAAERLFALIKHEHQRTSRAILELTGE
                     SKLCERFEGLQRRFERVRPMVDQANRWQVELLRDTRHNGGGEKLMMPLLMTMNCVAAG
                     LGWTG"
BASE COUNT          527 a          842 c          776 g          505 t
ORIGIN      
        1 ttgatgaaga actgcttggt gacctgccac cacttttcct gcatcagggg tagatagaaa
       61 aacgtcttcc cgacagcagc atcgttcatc atgatgaagc tgaagaagcc cagggcaatg
      121 agcgcgaacg cgccccagcc gaaggtgatc cagcccaact cgcgcgaggc tgcgccatcg
      181 attcgcgctg tcacgatatc gccgccagcg acggcagcat tggcgctgcg cgccagcttc
      241 agtgcgcgaa gcgctggcca gaacagcagc gccacggcgg cgatgacggt ccagaacagg
      301 atctgccagg cgcctgagta agcaccgaga tgctccagcg cgccgcggat cagacctgtc
      361 gcgccgaagg ccgcaagcac acagaacagc gcaaacagaa tggcggcgat ggccagaccg
      421 tctgcgcgcg gaggtattga tggcgcttga gtggacgtca caggaacggt ggccctctcc
      481 gctgcatgcg gaaagggcct tgttcattac ggattgaagt agggaaccgt cgccgggtcc
      541 ttgccccagg ccgcggccag atacttggca ccaagcttgg caacggtgcc gtcatcgatc
      601 atcgactgga tgatcttgtc gagtgttgcg ttgttcggat tgcctttcgg gtagattgca
      661 ccataggtct cgccggtctt gtactgaccg atcacagcca ccttgcccgg attggccact
      721 tcctcggcaa gcacgatcga cgtatccgtc atcgctgcat cgatctggcc ggcacggagt
      781 gcagtgaaca tgtcgccctg atccgggtaa acctgcacgt cggtcaggcc gagcgtaccc
      841 tgtgcgaatg ccgcgcccgt ggtggcctgc tggacgccaa ccttggccga cttgatcgtc
      901 ttttcgtcaa ccggcgcgtc cgtccgtgcc aacacaccga tatcggagtt gaagtatgga
      961 accgagaaat cgtgcacctt ctttcgatct tcggtgatcg agatctgcga catggccaga
     1021 tcgaaatcgg cggtctgccc ggcgatcagg gcgtcccatc cgacattgac gacttccagt
     1081 ttgtcatatc cggcgcgcca ggcgatttcg gcggccatgc agtattccat gccgtccttg
     1141 atggcttcgg gcgtatcgcc attccaccag atcggcgcag gcagagacac ctcaacagtc
     1201 aactggccgg ccttcgcggg cttctcgatc gggatcgagc ccttggcgcc cgacagctcg
     1261 cagttgccga tcatgtcggc tgcggcggcg ggtccggcaa gggcgatggc cgcagcggcc
     1321 gcaagcagaa ttctcttcgt gatgttcatg gttctacaac tccctgttct tgtaaccatc
     1381 gctgaaacat aaaccgaaat cggagcagtt gggcaagccc ctcgttgcgc tgccatttag
     1441 gcccccattc gccccctacc ctgtccatcc caggcccgct gcgacgcagt tcatggtcat
     1501 cagcaagggc atcatcagct tctcgccgcc accattgtgg cgggtatcgc gcagcagctc
     1561 gacctgccag cggttggcct gatcgaccat cgggcgcacg cgttcaaacc ggcgctgtag
     1621 cccctcgaaa cgctcacaga gtttggattc gcccgtcagt tcaagaatcg cgcggctcgt
     1681 tctctgatgt tcatgcttta tcagggcgaa gagccgttca gcggccgttc tgtcctcgac
     1741 aagctctgca tactgttcgg caaccgacat atcggcaagg taaagtgact tctcgacttc
     1801 atcgacggcg agacggaaac cgcgcgaccg gttgtacatc tcacgcaaca gcttcagtcc
     1861 ttcgccgcct ctcggaacaa gaaaatcgtc aaaggcgtat ccgaggccat accagccggt
     1921 cagcagatgc cggttctgac tccaggcaaa gacccacgga atggcccgca gatcgccaat
     1981 gcctttggcg ccgaaacgcc gcgccggacg ggagccgagc ttgagattgg ccagttcctc
     2041 cactggcgaa gcctgatgga agtaagccag cagagcgggg tcttcggcga gcttccggta
     2101 gtggcggaag gaagcaccgg caatccactc gacagcctct tgatgttcgg caacaacctt
     2161 gagctccgct tcgtcgatgg acttcaacgt gtgaacaaac acgctggcgg cgagaacctc
     2221 gagattgtgc tgagccgttc cccggtttgc gaacttggac gagaccacct caccctgttc
     2281 ggtgacccgc atgcgcccat ttatggtgcc agcgggctgg gcggcgatgg ctcggccggc
     2341 gggcgcgccg ccgcggctga ccgaaccccc acgcccatgg aagaagctga cctccacgcc
     2401 ggcatctcgg ccaacgcgga tcagccgccg ttgcgcctcg aagagttcga agctggcgca
     2461 aaagaagcct ccatccttgt tcgagtccga gtagccgagc atgatttcct ggcgcccgcc
     2521 attggctgca atcgacccct tgacgaccgg aaccttcagc agctccgtca tgatcgcagg
     2581 tccggcccga aggtcatcga tggtctcgaa aagcggcacc acccgaatgc ggcaagcctc
     2641 acgggcggtc
//
LOCUS       DBNGVR010000088         2625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3763791, whole genome shotgun sequence.
ACCESSION   DBNGVR010000088 DBNGVR010000000
VERSION     DBNGVR010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3763791"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="AC16A0_01955"
     CDS             complement(<1..434)
                     /locus_tag="AC16A0_01955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR3104123.1"
                     /translation="MTDATVSITAMGRRAEGLAMHEGHPLFVPLSLPGEELRVAIDGE
                     RGSIRDILAASPQRIAPFCPHYGRCGGCQLQHWQEEPYRAWKSGLVASAFAAKGIDTN
                     VSRLIDAHGAGRRRVSIHARRRDGVATAGFMAARSHDLLDLDR"
     gene            complement(427..1068)
                     /locus_tag="AC16A0_01960"
     CDS             complement(427..1068)
                     /locus_tag="AC16A0_01960"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005765897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR3104124.1"
                     /translation="MTSFVILLGGDLTVTARLRQQIRGARAIAADSGMMHASALGLVP
                     ELWVGDFDSAGSELAIQYAEVPREIFAVEKDATDGAIAVAAAIARGASRIVLAGGLGG
                     QADHMAGLLGQALQIARGGRSCLVTSGAEEAYPLLPGRMLIDLPAESRISLVPFADLE
                     GLDLAGVKWPLQSRHVALGSTLTLSNVALGPVTVGLRGGHGVAIAMPRFDSHD"
     regulatory      1126..1231
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1278..2273
                     /gene="thiB"
                     /locus_tag="AC16A0_01965"
     CDS             1278..2273
                     /gene="thiB"
                     /locus_tag="AC16A0_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HIR3104125.1"
                     /translation="MKMIFATILTLALAAIPAAAKQTLTVYTYDSFVSEWGPGARIKA
                     AFETDCDCSIDWVALGDGVAVLNRLKLEGAATKADVVLGLDTNLTAEAAATGLFGPHG
                     LDPALAKVPVAWSDKLFMPFDYAHFAVIYDSETMKNPPTSLDDLVNGDPAQKIILQDP
                     RSSTPGLGFLLWMKAVYGDKAGEAWAKLSKRILTTTPGWSEAYALFTKGEAPMVLSYT
                     TSPAYHAIAENSTRYKAAAFKEGHYLQIEVAGLIEASPEKELARKFLAFMMSPGFQDQ
                     IPTSNWMLPAAPTSAPLPAAFDTLVKPEKTLLFTPEEVNASRRAWIDEWLNALAK"
     gene            2270..>2625
                     /locus_tag="AC16A0_01970"
     CDS             2270..>2625
                     /locus_tag="AC16A0_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HIR3104126.1"
                     /translation="MTGAGKHLPAWVALAAIGAALAAGFLPVLWLGLADGAGAFDAYV
                     LRVLQFTLLQAGLSTLLSLILGLALARALARQDFPGRALVLRLLNLPLALPAIVVIIG
                     IVGVYGRSGWLGGAVAL"
BASE COUNT          437 a          909 c          867 g          412 t
ORIGIN      
        1 cggtcgaggt cgagcaggtc atggctgcgc gccgccatga agcccgccgt cgcaaccccg
       61 tcgcgccgcc gcgcatgaat gctgacgcgc cggcgcccgg cgccatgggc gtcgatcagt
      121 ctactgacgt tcgtgtcgat ccccttcgcc gcgaatgcag aagccaccaa cccggacttc
      181 caggcgcgat agggctcttc ctgccagtgc tgcagctggc aaccgccgca gcgcccgtaa
      241 tgcggacaga agggcgcgat ccgctgcggc gaggccgcga ggatgtcgcg gatgctgccg
      301 cgctcgccat cgatggcgac ccgcagctcc tcgcccggca gactcagcgg cacgaagagc
      361 ggatggccct catgcatcgc aaggccttcc gcgcggcggc ccatggccgt gatcgagacg
      421 gtggcgtcag tcatggctgt cgaaccgcgg catggcgatg gcgacgccat ggccgccacg
      481 cagccccaca gtcaccgggc cgagcgcgac attggacagc gtgagtgtcg accccaacgc
      541 cacatggcgc gattgcagcg gccacttgac gcccgcaagg tcgagccctt cgagatcggc
      601 gaagggaacg aggctgatgc ggctctcggc gggaagatcg atgagcatgc gccccggcag
      661 cagcggatag gcttcctcgg caccgctggt gacgaggcag gagcggccgc cccgcgcgat
      721 ttgcagcgcc tggccgagaa gcccggccat gtgatcggcc tgcccgccaa gaccgcccgc
      781 cagtacgatg cgcgaggcac cgcgcgcaat tgcagcggcg acggcgatgg cgccgtccgt
      841 tgcatccttt tccactgcga agatctcgcg tggcacctcg gcatactgga tggcgagttc
      901 ggaccccgcg gagtcgaagt cgccgaccca gagttccggc acgaggccga gggctgaggc
      961 atgcatcatg ccgctgtcgg cggcgatggc gcgggcgccg cggatctgct ggcgcagccg
     1021 cgccgtgacc gtgagatcac cgcccagcag gattacgaag ctcgtcatgg cgccttccta
     1081 gcaaatgccg cttgcaagcg catccgcgct gacatagatc tctcgctgct ctcaacgggg
     1141 tgccagacga ctggctgaga ggggctttcc gcctcaaccc gctgaacctg atccggatca
     1201 tgccggcgga gggattgcga gcggaccccg atgtcctggg tccaatcctc catctgccgg
     1261 cgcatggagg tagaccgatg aagatgatct tcgcaaccat cctgacgctg gccctagcgg
     1321 ccatacccgc cgcagcgaaa cagacgctga ccgtctatac ctatgacagc ttcgtctccg
     1381 aatggggccc gggcgcccgc atcaaggcgg ctttcgagac ggactgcgac tgcagcatcg
     1441 actgggttgc cctgggcgat ggcgtggccg tgctgaaccg cctgaagctg gaaggtgccg
     1501 cgaccaaggc cgatgtcgtg ctgggcctcg acaccaacct caccgccgag gccgcggcca
     1561 ccggcctctt cggcccgcac ggactcgatc cggccctggc gaaggtgccc gtggcatgga
     1621 gcgacaagct cttcatgccc ttcgactacg cgcatttcgc ggtgatctac gacagcgaga
     1681 caatgaagaa tccgccgact tcgctcgacg acctcgtcaa cggcgacccg gcgcagaaga
     1741 tcatcctgca ggacccgcgc agctcgacgc ccggcctcgg cttcctgcta tggatgaaag
     1801 cggtctatgg cgacaaggcc ggtgaagcct gggccaagct ctcgaagcgc atcctgacca
     1861 caacgcccgg ctggtcggag gcctatgccc tcttcaccaa gggcgaggcg ccgatggtcc
     1921 tctcctacac gacctcgccc gcctatcacg ccattgccga gaacagcacg cgctacaagg
     1981 cggcggcttt caaggagggc cactatctgc agatcgaggt ggcgggactg atcgaggcct
     2041 cgcccgagaa ggagctggcg cggaagttcc tcgccttcat gatgtcgccg ggcttccagg
     2101 accagatccc caccagcaac tggatgctgc cggccgcgcc gacctcggca ccactgcccg
     2161 ccgccttcga cacgctggtg aagccggaaa agacgctgct cttcacgccg gaagaggtca
     2221 atgccagtcg ccgcgcctgg atcgacgagt ggctaaacgc gctggccaaa tgaccggggc
     2281 cggcaagcac cttcctgcct gggtcgcact cgccgcgatc ggcgcggcgc tggccgcggg
     2341 tttcctgccg gtgctctggc tggggctggc cgacggagcg ggcgccttcg atgcctatgt
     2401 gctgcgcgtg ctgcagttca cgctgctgca ggcgggcctc tcgacgctcc tgtccctgat
     2461 cctcggactg gccctggcgc gggcgctggc gcggcaggat ttcccgggcc gcgcactggt
     2521 actgcggctt ctcaatctgc cgctggccct gccggccatc gtggtgatca tcggcatcgt
     2581 gggcgtctat ggacgcagcg gctggctggg cggcgccgtc gcgct
//
LOCUS       DBNGVR010000089         3561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_227257, whole genome shotgun sequence.
ACCESSION   DBNGVR010000089 DBNGVR010000000
VERSION     DBNGVR010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_227257"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="AC16A0_01975"
     CDS             complement(<1..936)
                     /locus_tag="AC16A0_01975"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HIR3104127.1"
                     /translation="MDKRVNQGILPAKAIADYCSTGRIRLAAPADADQIQPASLDLRL
                     GPWACRVRASFLPGKRSTVAERLKELELHRVALTDGAVLETGCVYIVPLQESLALPKG
                     VSATANPKSSTGRLDIFTRVITDHAQEFDTVPEGYHGPLYAEISPRTFPILARSGSRL
                     SQIRFRAGASLADDALIRALHAKEPLISSGEANIDNGLALSVDLSSFDADRPVGFRAK
                     RHAGLIDVDRKDALDVLDYWEPIWQRGSLILDPDQFYILASKEAVRVPPAYAAEMVPF
                     NPLVGEFRVHYAGFFDPGFGHSAGHGEGARAVLEVR"
     gene            1024..1416
                     /gene="apaG"
                     /locus_tag="AC16A0_01980"
     CDS             1024..1416
                     /gene="apaG"
                     /locus_tag="AC16A0_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR3104128.1"
                     /translation="MYERITRGIKVIVRPQYLEGQSKPDEDHFVWAYTITVENHGRET
                     VTLKTRYWKITDATGKMQEVRGAGVVGETPTLKPGDSFTYTSGCPLKTPSGFMVGAYQ
                     MQNEAGELFNIEIPAFSLDSPHDRHAIN"
     gene            1426..2583
                     /gene="argE"
                     /locus_tag="AC16A0_01985"
     CDS             1426..2583
                     /gene="argE"
                     /locus_tag="AC16A0_01985"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIR3104129.1"
                     /translation="MTGLHVTTEEMLARLVAFDTTSRDGNIPLVEFVESYLDGWDVPH
                     FRVDYEAGRKTNLFATIGPEIAGGIVLSGHTDVVPVDGQDWTSDPFALTARDGRLFGR
                     GTCDMKGFLAVCLAMVPRFKAARLKSPIHLALSCDEEVGCKGVRPLVAHLRDHLPKPR
                     AVIVGEPTSMKVVNAHKSAITFSTDVTGHEAHSSLIDQGVNSIMVAGELLSELNRIRA
                     DLVARGDPGHRFNPPYSTLHVGLIAGGTAKNIIPKRCSFQWETRLLPGADPEEVPLRF
                     ARFAKALEPAMKAVAPEAGIATVCANSVPGLAPEKDSPAEHLALHLANANSTHAVSYC
                     TEAGLFQQIGIPAVICGPGSIEQAHKPDEYIDVSELRKCEVFMEKLVERLI"
     gene            2754..3520
                     /locus_tag="AC16A0_01990"
                     /pseudo
     CDS             2754..3520
                     /locus_tag="AC16A0_01990"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          695 a         1153 c         1127 g          586 t
ORIGIN      
        1 gcgcacctcc agcaccgcgc gcgcaccttc gccatggccg gcgctgtgcc cgaaaccggg
       61 atcgaagaaa cccgcataat gcacgcggaa ttcgcccacc agcggattga agggcaccat
      121 ctcggcggca taggcgggcg gcacccgcac ggcctccttc gaggcaagga tgtagaactg
      181 gtcgggatcg aggatcagcg aaccgcgctg ccagatcggc tcccaatagt cgagcacgtc
      241 aagcgcgtcc ttgcggtcga cgtcgatgag cccggcatgg cgcttggcgc ggaagcccac
      301 cggccggtcc gcgtcgaagc tggagaggtc gaccgagagc gccaggccat tgtcgatgtt
      361 ggcctcgccc gacgagatca gcggctcctt cgcatgcagg gcgcggatca gcgcgtcgtc
      421 ggccagcgag gcgccggcac ggaagcggat ctgcgacagg cgcgagccgc tgcgcgccag
      481 gatgggaaag gtgcgcgggc tgatctcggc atagagcggg ccgtgatagc cctccggaac
      541 cgtgtcgaat tcctgggcat gatcggtgat gacccgggtg aaaatgtcca gccgcccggt
      601 ggagctcttc ggattggcgg tggccgagac ccccttcggc agggcgagcg actcctgcag
      661 cggcacgata tagacgcagc cggtttccag caccgcgcca tcggtcagtg caacccgatg
      721 caactccagt tccttgagcc gctcggcaac cgtgctgcgc ttgccgggca ggaaggaggc
      781 gcgaacccgg caggcccagg gcccgagccg gagatcgagg ctggccggct ggatctggtc
      841 ggcatcggcg ggtgctgcga gccgaatccg gccggtgctg caatagtctg caatggcctt
      901 ggccggcagg atgccctggt tcacgcgctt gtccatccgg tcatgtggcg gatttggccc
      961 tgactgcgca agggcttcag caatgccggt ggaaaacccg gcgaaacggg ctagactggc
     1021 agcatgtacg aacgcatcac gcgcggaatc aaggtcatcg tccggccgca atatctcgaa
     1081 ggccagtcga agcccgacga ggatcacttc gtctgggcct ataccatcac ggtcgaaaac
     1141 cacggccgcg agacggtgac gctgaagacc cgctactgga agatcaccga cgccaccggc
     1201 aagatgcagg aggtgcgcgg tgccggcgtg gtgggcgaaa cgccgacgct gaagcccggc
     1261 gacagcttca cctacacctc cggctgcccc ctgaagacgc cctcgggctt catggtcggc
     1321 gcctatcaga tgcagaacga ggcgggcgaa ctcttcaaca tcgagatccc ggccttctcg
     1381 cttgacagcc cgcacgaccg ccacgccatc aactgatcgg acatcatgac cggactgcac
     1441 gtcaccaccg aagagatgct tgcgcgcctt gttgccttcg acaccaccag ccgcgacggc
     1501 aacattccgc tcgtcgaatt cgtcgagtcc tatctcgacg gctgggacgt gccgcatttc
     1561 cgggtcgact acgaggcggg caggaagacc aatctcttcg ccaccatcgg gccggagatc
     1621 gcgggcggca tcgtgctctc gggccacacc gacgttgtcc cggtggacgg ccaggactgg
     1681 accagcgatc ccttcgcgct gacggcgcgc gacggccggc tcttcggccg cggcacctgc
     1741 gacatgaagg gcttcctcgc cgtctgcctc gccatggtgc cgcgcttcaa ggcggcgcgg
     1801 ctgaaatcgc ccatccatct ggcgctctcc tgcgacgagg aagtgggttg caagggcgtg
     1861 cgcccgctgg tggcgcatct gcgcgatcat ctgccgaagc cccgcgccgt gatcgtcggc
     1921 gaaccgacct cgatgaaggt ggtcaacgcg cacaagagcg ccatcacctt ctccaccgac
     1981 gtcaccggcc acgaggcgca ctccagcctg atcgaccagg gcgtcaattc catcatggtg
     2041 gcgggcgaac tgctgtccga gctcaaccgc atccgcgccg atctcgtcgc gcgcggcgat
     2101 cccggccacc gcttcaaccc gccatactcg acgctgcatg tgggcctgat cgccggcggc
     2161 acggccaaga acatcattcc gaagcgctgc agcttccagt gggagacccg gcttctgccc
     2221 ggcgccgacc cggaagaggt gccgctgcgc ttcgcgcgct tcgcgaaggc gctcgagccc
     2281 gccatgaagg cggtggcccc cgaggccggc atcgccactg tctgcgccaa cagcgtgccg
     2341 ggcctcgcac cggaaaagga ttcgcctgcc gaacacctgg cgctgcatct ggccaatgcc
     2401 aattccaccc acgcggtcag ctattgcacc gaagccggac tgttccagca gatcggcatc
     2461 cccgccgtca tctgcggccc cggctccatc gagcaggccc acaagccgga tgaatacatc
     2521 gacgtctccg aactccgcaa atgcgaggtc ttcatggaga agctggtcga gagattgata
     2581 tagaaagaac ccttctcccg ctcgcgggag aaggtggcgc gcagcgccgg aagagagccg
     2641 ccttgcgcag cagggcggtg atgctaggcc gtgttgacaa agtggattca caaatcacct
     2701 gagttctgat tcaaggctgt tctttgcgga gagcagcagt ggttcgtgga ctgatgtcgg
     2761 acaaggaatg ggcgtatttc gaaccatttg tcatcgagac aggaccgaag cgtgggcggc
     2821 ccccgggcga tcatcgcctg gtgctggatg gcgtgttctg gattgccagg acaggcgcgc
     2881 cttggcggga tttacccgag gagttcggca agtggggttc cgtctaccgt cagttccgcc
     2941 gctggtccgt tgccggggtc tgggatgcga tgctggaggc attgaacgct accggcatag
     3001 cgccaagcag cgtgcagatg atcgactcca ccatcgtccg cgctcaccag catgcatcgg
     3061 gcgcaaaaag ggggctcatc aggagagtat tgggcgctcg cgaggtggcc agacgaccaa
     3121 gatccatctg agaaccaatt ccattggcct gcccatcgct gtgactctgt ccagcggaga
     3181 ggcatcagat tacaagggat atgagccctt gatgttggaa ccagggccag agcccaaggt
     3241 gttgatcgcc gacaaaggct acgatagcga tgccatccgc gaggatctga tcgccagaat
     3301 ggcggagccg gtcattccca tgcgccggaa ccgcaaggca caagatacaa tcgatggcgc
     3361 catctacacc ctcaggaacc tgatcgagcg ctgcttcaac aaactcaagc actcccgcag
     3421 gcttgcaaca cgatacgaca agacaacaga gagctatctc ggcttcgttc tcatcgcctc
     3481 agcacgcctt tggtgcaggc actttgtcaa cacggcctag aaacagaccc ccacgctcgg
     3541 attggacgat ctaattgatg c
//
LOCUS       DBNGVR010000090         3302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2389599, whole genome shotgun sequence.
ACCESSION   DBNGVR010000090 DBNGVR010000000
VERSION     DBNGVR010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2389599"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="AC16A0_01995"
     CDS             <1..318
                     /locus_tag="AC16A0_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104130.1"
                     /translation="PSGAETTMDRGQLRIMSTVRTQDDVLCREFEVDAIDTGQTTLGV
                     ACQTEGQWRLEIAVAAPASAEGFAPASSLSTVTSYLDAIGATDPLESDAEEKALKGLR
                     EVP"
     gene            complement(329..1519)
                     /gene="choV"
                     /locus_tag="AC16A0_02000"
     CDS             complement(329..1519)
                     /gene="choV"
                     /locus_tag="AC16A0_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015220 - choline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015871 - choline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter ATP-binding protein"
                     /protein_id="HIR3104131.1"
                     /translation="MTTAVEFKNVDIIFGDRSAEALAMARDGADRSDILKKTGAVLGA
                     TGANLTVNEGEISVLMGLSGSGKSTLLRAVNGLNKVTAGEVLVKDGDRMVDVASCNEA
                     TLRHIRQKQVAMVFQQFALLPWRTVRQNAGFGLELSGMPEAERNDKVDRQLRLVGLDQ
                     WANKYVHELSGGMQQRVGLARAFATEAPILLMDEPFSALDPLIRTKLQDELLQLQKAI
                     KKTIIFVSHDLEEALKIGSRITIMEGGRIVQSGAPEDIVLRPANDYVRDFIANVNPLS
                     VLTAWNVMRSLHELEPAGEGWIWLDKRKTTRFKLDKDQKVVAAERDKKKATWVSCDDV
                     EKPIPPDTRPVYWARPGTSLKTVMLAIHNTDVAPVAIFDEADRFIGAIGVRNVLQAVL
                     KRSD"
     gene            complement(1516..2370)
                     /gene="choW"
                     /locus_tag="AC16A0_02005"
     CDS             complement(1516..2370)
                     /gene="choW"
                     /locus_tag="AC16A0_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter permease subunit"
                     /protein_id="HIR3104132.1"
                     /translation="MDPVSELVRAWKIPVGKWGKALIDFIVEYFQWLFDAIKISLNFV
                     IENATAFLLLLPPFVLALALAGFAYWLQKSWKLALGVLLGLLFIMNQGLWKETVQTLV
                     LVVGATALSMAIGVPLGIWAAHKPKVWRVLQPVLDLMQTMPTFVYLIPILILFGLGAA
                     PALIVTIIFAMPAPVRMTYLGITSIPKPMIEAGESFGATKRQLLWKVELPAALPSIMA
                     GLTQCIMLSLSMVVFATLIGAPSLGNPVNKALANRNIPLGIEAGLAIVILAIILDRVL
                     AMKAGGKK"
     gene            complement(2480..>3302)
                     /gene="choX"
                     /locus_tag="AC16A0_02010"
     CDS             complement(2480..>3302)
                     /gene="choX"
                     /locus_tag="AC16A0_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0033265 - choline binding [Evidence IEA];
                     GO_process: GO:0015871 - choline transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="choline ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3104133.1"
                     /translation="WTDIQVTTGATSVVLEALGYQPEVSTLSVPVTYASLKNKDIDAF
                     LGNWMPSMTADIQPYLDDKSVEQLATNLEGAGYGLVVPQYVADAGVKSLADLGANAEK
                     FGGKIYGIEAGNDGNRIIQTMIDDAKNNLSGFTMVESSEAGMLTEAEKAIKNNEWIVF
                     LAWTPHPIMGEMKIHYLDGMGDSGFGAATVLTNVRAGYTSDCPNAGKLLANLKFDLAM
                     EGAMMAPVLKDGADPKETARAWLKANPDAVKPWLEGVTTFDGGDAAAAVSAALGG"
BASE COUNT          606 a         1030 c         1048 g          618 t
ORIGIN      
        1 ccttccggag cggaaaccac aatggaccgt gggcagcttc ggatcatgtc caccgtcaga
       61 acgcaagacg acgtgctttg ccgggaattc gaagtcgacg cgatcgacac gggccagaca
      121 acgctcggcg tcgcctgcca gacggaaggg caatggcgac tggagattgc ggtcgccgca
      181 ccggcatcgg cagaaggctt cgcgccagcc tcctcgctat cgaccgtgac atcctatctc
      241 gacgcgatcg gcgcaacgga tcccctggag tccgatgccg aagagaaagc gctcaagggc
      301 ctgcgcgagg tgccttgaac ccgcttggct agtccgagcg cttcagcacg gcctgcagca
      361 cgttgcggac gccgatggcg ccgatgaagc ggtcggcctc gtcgaagatt gcaacgggtg
      421 ccacgtcggt gttgtggatg gccagcatga cggtcttgag cgacgtgccg ggccgcgccc
      481 agtagaccgg ccgcgtatcg ggcgggatgg gtttctcgac atcgtcgcag gagacccagg
      541 tggccttctt cttgtcgcgc tcggcggcga cgaccttctg gtccttgtcg agcttgaagc
      601 gcgtggtctt gcgcttgtcg agccagatcc agccttcgcc cgccggctca agttcatgca
      661 gggagcgcat cacgttccag gcggtcagga ccgacagcgg attcacattg gcgatgaagt
      721 cgcgcacata gtcgttggca gggcgcagca cgatgtcctc gggggcgccc gactgcacga
      781 tgcggccgcc ttccatgatg gtgatgcggc tgccgatctt gagcgcctcc tcgagatcgt
      841 ggctcacgaa gatgatggtt ttcttgatcg ccttctgaag ctgcagcagc tcgtcctgca
      901 gcttggtgcg gatcagcgga tcgagcgccg agaagggctc gtccatcagc aggatggggg
      961 cttccgtcgc aaaggcgcgg gcgaggccca cacgctgctg catgccgccg gacagctcgt
     1021 gaacgtattt gttggcccat tggtcgagac cgaccagccg gagctggcgg tcgaccttgt
     1081 cgttacgctc cgcttcgggc atgcccgaga gttccaggcc gaagccggca ttctggcgca
     1141 ccgtgcgcca gggcagcagg gcaaactgct ggaagaccat ggccacctgc ttctgacgga
     1201 tgtgacgcaa cgtggcctcg ttgcaggagg cgacgtcgac catgcggtcg ccatccttca
     1261 ccaggacttc gcccgcagtg accttgttga ggccattcac ggcgcgcaga agcgtcgact
     1321 tgccggaacc cgagagcccc atcagcacgc tgatctcgcc ttcgttcacc gtaagattgg
     1381 cccccgttgc gcccagcacc gcaccggtct tcttgaggat gtccgaacgg tctgcgccgt
     1441 cgcgcgccat ggccagtgcc tcggcagacc tgtcgccgaa gatgatgtcg acgttcttga
     1501 actcgacagc ggtggtcatt tctttcctcc cgccttcatg gccagaacac gatcaaggat
     1561 gatggcgagg atgacgatgg ccaggcccgc ctcgataccc agcggaatgt tacggttggc
     1621 cagcgccttg ttcaccggat tgccgaggct tggtgcgccg atcagcgtgg cgaagacgac
     1681 catcgacagc gacagcatga tgcattgcgt gaggcccgcc atgatcgacg gcagcgccgc
     1741 cggcagctcg accttccata gcagctggcg cttggtggct ccgaagcttt cgcctgcctc
     1801 gatcatcggc ttcgggatcg aggtgattcc gagataggtc atgcgcaccg gcgccggcat
     1861 ggcgaagatg atggtgacga tcagggcagg tgctgcgcca agcccgaaga ggatcagaat
     1921 gggaatgaga tagacgaagg tgggcatggt ctgcatgagg tcgaggaccg gttgcagcac
     1981 ccgccagacc ttcggcttgt gcgcggccca gatcccgagg ggaacgccga tggccatgga
     2041 cagggcggtg gcgccgacga ccagcacgag cgtctgcacc gtctccttcc acagtccctg
     2101 gttcatgatg aagagcaggc ccagcagcac gccgagcgcc agcttccatg acttctgcag
     2161 ccagtaggcg aagccggcca gcgcgagcgc cagcacgaag ggcggcaaaa ggagaaggaa
     2221 tgcggtggcg ttctcgatga cgaaattcag cgagatcttg atggcatcga agagccattg
     2281 gaagtactcg acgatgaagt cgatgagcgc cttgccccac ttgccgacgg gaatcttcca
     2341 ggcgcgaacg agttctgaga ccggatccat gtgcccctcc ccaagggttg ttgcccttcc
     2401 gcagtcttga gcgctgcttg tgcgaaggga tcataggtcc cgggccggct tgcggccagc
     2461 ccggaacgta aggagcgtat cagccgccga gcgctgcgga aaccgccgcc gccgcatcgc
     2521 caccgtcgaa ggtggtgacg ccttcgagcc agggcttcac ggcatccgga ttggccttga
     2581 gccaggccct ggcggtttcc ttcggatccg ccccgtcctt cagcacgggc gccatcatgg
     2641 cgccttccat ggcgaggtcg aacttgaggt tggcgagcag cttgccggca ttcggacaat
     2701 cgctcgtgta gcccgcgcgc acattggtca ggaccgtcgc tgcgccgaag cccgaatcgc
     2761 ccatgccatc gagataatgg atcttcatct cgcccatgat cggatgcggc gtccacgcca
     2821 ggaacacaat ccactcattg ttcttgatgg ccttttcggc ctcggtcagc atgccggctt
     2881 ccgagctttc caccatcgtg aagcccgaga gattgttctt cgcatcatcg atcatcgtct
     2941 gaatgatgcg gttaccgtca ttgccggcct cgatgccgta gatcttgccg ccgaacttct
     3001 cggcatttgc gccaagatcg gccaatgact tcaccccggc gtccgccaca tattgcggga
     3061 ccacaagacc gtagcccgca ccttcaagat tggtcgccag ctgctctacc gacttgtcat
     3121 ccagataggg ctggatatcg gccgtcatgc tgggcatcca gttgccgagg aaggcatcga
     3181 tgtccttgtt cttgagcgag gcgtaggtga cgggcaccga cagggtggac acttccggct
     3241 ggtagccgag cgcctcaagc accaccgagg tggcgcccgt cgtcacctga atgtccgtcc
     3301 ag
//
LOCUS       DBNGVR010000091         4082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1997661, whole genome shotgun sequence.
ACCESSION   DBNGVR010000091 DBNGVR010000000
VERSION     DBNGVR010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1997661"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..471
                     /locus_tag="AC16A0_02015"
     CDS             10..471
                     /locus_tag="AC16A0_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104134.1"
                     /translation="MARTRRFAGEADLVVWLWSRDVVGSDVVAEGVRPDMVVETKSDL
                     EPRPSDGRESNRISTLSGEGMAAFVDRLSDFVKARVGLGEAAVIVRERQIQAVEESIR
                     FLNDALRQPTGALELVSADLRGAATALARLTGRIDVEDWLDDIFSRFCIGK"
     gene            537..2402
                     /gene="mnmG"
                     /locus_tag="AC16A0_02020"
     CDS             537..2402
                     /gene="mnmG"
                     /locus_tag="AC16A0_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR3104135.1"
                     /translation="METSFDVIVVGGGHAGVEAAAAAARLGARTALLTHAFATVGEMS
                     CNPAIGGLGKGHLVREVDAMDGLMGRVADRAGIQFRLLNRSRGPAVRGPRAQADRRLY
                     RAAMQDEIRQQPNLTVIEGAALGFTLKNGRLSAITTEDGRAFSCAAAVLTTGTFLNGL
                     IHLGEKTTPAGRFGEKPSHGFSAQLAAAGLALARLKTGTPARLDGRSIDWGVLEEQRG
                     DADPVPFSMMTSAITTPQVSCFITHTTAETHDIIARNIARSPLFSGQIQGAGPRYCPS
                     VEDKIHRFRDRSSHQIFLEPEGLDDPTVYPNGISTSLPEDVQEAFLRSIPGLSRVSIL
                     RWGYAIEYDHVDPRELIHSLECKRIPGLFLAGQINGTTGYEEAAAQGLLAGANAARQA
                     GGQDPVILDRGRAYAGVMIDDLVTRGVSEPYRMFTSRAEYRLSLRADNAWERLTPLGI
                     ELGLVGNGRAEAYRVRQQEIDDARALARGLTLTSAQAARQGLKVNQDGKSRSVLDLVA
                     MPEIGFAGACRVWPELGAIPSHAVEALEADALYAGYLGRQDEEIRTLRKEEQLCLPDG
                     LDYAALPSLSAELRQKLQRVQPATLGQAARIDGMTPAALSVILAHVRRPSLRRSA"
     gene            2406..3038
                     /gene="rsmG"
                     /locus_tag="AC16A0_02025"
     CDS             2406..3038
                     /gene="rsmG"
                     /locus_tag="AC16A0_02025"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3104136.1"
                     /translation="MQPFDLLRPYDVSRESCQRLEAYVDLLLAWQKRINLIGPSTAEQ
                     VWTRHILDAVQLMPLLPPGSLALADLGSGAGIPGLVLAIAGGHEAHLYESNGKKAAFL
                     GQAIRTTGAKARVHQVRLEDLPGHPALPRVGVVTARALAPLPLLLDYAEPFLEAGAIG
                     LFHKGQDVDNELTEATKYWKLQVKKLPSITDSRGVILEVREASRVQRPDR"
     gene            3016..3849
                     /locus_tag="AC16A0_02030"
     CDS             3016..3849
                     /locus_tag="AC16A0_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3104137.1"
                     /translation="MSNAQTDRFGAETPRILAVANQKGGVGKTTTAINLGTALAAVGE
                     TVLIIDLDPQGNASTGLGIARRNGQTSTYHVLIGEAALADVIQESGIPRLHCAPSTMD
                     LLGAELELASFDRKTYRLADAIKAMTGSASAGRSYSYILVDCPPSLNLLTINSLSAAD
                     AVLVPLQCEFFALEGLSQLLKTVERVKTTLNPRLSIQGVVLTMFDKRNSLSEQVAEDV
                     RSVLGDKVYQTVIPRNVRVSEAPSHGKPVLLYDNSCMGSQAYIRLASEVIRRERTLRA
                     A"
BASE COUNT          713 a         1355 c         1299 g          715 t
ORIGIN      
        1 cggcaaggca tggcccgcac gcggcgcttc gccggcgagg cagatctggt ggtatggctg
       61 tggtcgcgcg atgtcgtggg aagcgatgtt gttgccgaag gcgtcaggcc tgacatggtt
      121 gttgagacga agagtgatct ggagcctcgc ccttccgatg gtcgcgagtc gaatcggatc
      181 tcgacgctga gcggcgaagg catggcggcc ttcgtagacc gcttgtccga cttcgtgaaa
      241 gcaagggtag ggctgggcga agccgccgtg attgtccgtg aacggcagat ccaggccgtg
      301 gaagagtcga ttcggttcct caacgatgcg ctccgtcagc cgaccggtgc gctggagctt
      361 gtctccgccg atctgcgcgg tgcggccacg gcactggcgc ggctgaccgg gcgcatcgat
      421 gtcgaagatt ggctcgacga catcttcagc cgattctgca tcggaaagta aacgctgttt
      481 cacgtgaaac gcgtgatggc tttgacgcaa tcgcgcgtct gacctatgga tgcgccatgg
      541 aaacctcttt cgatgtgatc gtggtgggcg gcgggcatgc cggcgtggag gcggctgcgg
      601 cggcagcgcg gcttggcgcc cgtaccgctt tgctcacgca tgcattcgcc acggtcggag
      661 agatgtcctg caatccggcc atcgggggac tgggcaaagg tcacctggtg cgtgaggtcg
      721 acgccatgga tggtctgatg ggccgtgtcg ctgatcgcgc cggcattcag tttcgcctgc
      781 tcaatcgctc gcgcggaccg gccgttcgcg ggccgcgggc acaggccgat cgccgtctct
      841 atcgcgcggc gatgcaggat gagattcgcc agcagcccaa cctcacggtg atcgaagggg
      901 cggcgcttgg cttcacgctg aagaatggcc ggctctcggc aatcactacc gaagatggcc
      961 gggccttttc ctgcgctgcc gccgtgctca ccacgggaac atttctcaat ggcctgatcc
     1021 atctgggcga gaaaaccacg cccgcaggcc gcttcggcga gaagccgtca catggtttct
     1081 ccgcgcagct cgccgccgcc gggctggctc tggcgcggct caagacgggc acgccggcgc
     1141 ggctcgatgg ccgcagcatc gactggggtg tgctcgagga acagcgtggc gacgcggatc
     1201 cggtgccctt ctccatgatg acctcggcga tcaccacgcc gcaggtgtcc tgcttcatca
     1261 cccacaccac ggcggaaacc cacgacatca tcgcccgcaa cattgcacgc tcgccgctgt
     1321 tttcgggcca gatccagggc gcgggtccgc gctactgccc ctccgtcgaa gacaagatcc
     1381 accgtttccg tgaccggtcc tcgcaccaga ttttcctcga gccggagggc ctggacgatc
     1441 cgaccgtcta tcccaacggc atttccacct ccttgcccga ggatgtgcag gaagcctttc
     1501 tgcgcagcat tccgggcctg tccagggtca gcatcctgcg ctggggctat gccatcgagt
     1561 atgaccatgt cgacccgcgc gagctgatcc acagcctgga gtgcaagcgg attccagggc
     1621 tcttcctcgc aggccagatc aacgggacca caggctatga agaggccgcg gcccagggac
     1681 tgctggccgg cgccaacgcg gcgcgccagg ccggcggcca ggacccggtc atactggatc
     1741 gcggccgggc ctatgcgggc gtcatgatcg atgacctcgt gacccgtggc gtgagcgagc
     1801 cctatcgcat gttcacctcg cgggccgagt accggctcag cctgcgggcg gacaatgcct
     1861 gggaacgcct caccccgctt ggcatcgagc ttggtctcgt cggaaacggt cgcgctgagg
     1921 cctaccgggt ccgccagcag gagatcgatg atgcgagggc actggcgcgc ggcctgacgc
     1981 tgacctccgc gcaggccgcc cggcagggcc tgaaggtgaa ccaggatggc aagagccgct
     2041 cggttctgga tcttgtcgcc atgcccgaga tcggctttgc cggtgcctgc agggtctggc
     2101 cggagcttgg cgcgattccg tcccatgccg ttgaggcact cgaagcggac gccctctatg
     2161 ccggctacct gggccgccag gacgaggaga tccggacact gcgcaaggaa gagcagcttt
     2221 gcctcccgga tggactggac tatgcagccc tgcccagcct gtcggccgaa cttcgccaga
     2281 aattacagcg cgtacagccg gccaccctcg gacaggccgc gcggatcgac ggcatgaccc
     2341 cggctgcgct cagcgtgatc ctggcccatg tccgccgccc ctcgctccgc cggtccgcct
     2401 aggcgatgca gcccttcgat ctgctccggc cctacgatgt ttcacgtgaa tcatgccagc
     2461 ggctggaagc ctatgtcgat ctgctcctcg cctggcagaa gcgaatcaac ctgatcggac
     2521 cctcgaccgc cgagcaggtc tggacccgcc acatcctcga tgccgtccag ctcatgccgc
     2581 tgctcccgcc cggttccctt gccttggccg acctcggcag cggcgcgggc atacccggac
     2641 tcgtgttggc gattgccggc ggccatgagg cgcatctcta cgaaagcaat ggaaagaagg
     2701 cggccttcct cggccaggcc atccgcacga cgggcgccaa agcacgcgtc caccaggtcc
     2761 ggcttgaaga cctaccgggc catcccgctc ttcccagggt cggggtggtc accgcccggg
     2821 ccttggcgcc tctgccgctc ctgctcgact atgccgagcc cttcctggag gcaggcgcca
     2881 tcggcttgtt tcacaaggga caagatgtag acaatgaatt gaccgaggcc accaaatatt
     2941 ggaaactaca ggtgaagaaa cttccaagca tcaccgattc gcgtggcgtg attctggagg
     3001 tcagggaggc cagccgtgtc caacgcccag acagatagat tcggagccga aacacctcgc
     3061 attctggctg tcgccaacca gaagggcggc gtcggcaaga ccacgaccgc gatcaacctt
     3121 ggcacggccc tggctgcggt cggcgagacc gtcctcatca tcgacctcga tccgcagggc
     3181 aatgccagca ccggtctggg catcgcccgg cgcaacggtc agacctcgac ctaccacgtg
     3241 ctgatcggcg aggcggcgct tgccgatgtc atccaggaat cgggcatacc gcggctgcac
     3301 tgcgcgccat ccaccatgga tcttctgggt gccgaactgg agctcgcctc cttcgaccgc
     3361 aagacctatc ggcttgccga tgccatcaag gccatgacgg gttcagcctc tgccggccgg
     3421 tcctacagct acatcctggt ggactgcccg ccgtcgctca atcttctcac catcaattcg
     3481 ctctcggcgg ccgatgccgt gctggtgccg ctgcagtgcg agttcttcgc gctggagggc
     3541 ctgagtcagc tgctcaagac ggtggagcgg gtgaagacaa cgctcaaccc gcgtctctcc
     3601 atccagggcg tggtgctgac catgttcgac aagcgcaaca gcctgtcgga acaggtggct
     3661 gaagatgtcc gcagcgtgct gggcgacaag gtctaccaga cggtcatacc gcgcaatgtc
     3721 cgggtgtccg aggcgccctc ccatggcaag ccggtcctgc tctatgacaa cagctgcatg
     3781 ggctcgcagg cctatatccg gctggcctcc gaagtgatcc gccgcgagag gaccttgcgg
     3841 gcggcctgac cgattcaccc acgtaacaat caccggatgc ggcccgcgtc cggacggcaa
     3901 acggaatgcg agacatgatg caggcccaga agaaacgact aggccgcggc cttgccgcgc
     3961 tgatcgggga cgacacctcc gaggcggcgg tggtccagga catccgcagc ctgcgccata
     4021 tgccgatcga actgatccat gccagtccca acaatccgcg caagcatttc gccgacgccg
     4081 at
//
LOCUS       DBNGVR010000092         1794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2587238, whole genome shotgun sequence.
ACCESSION   DBNGVR010000092 DBNGVR010000000
VERSION     DBNGVR010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2587238"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="AC16A0_02035"
     CDS             complement(<1..286)
                     /locus_tag="AC16A0_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104138.1"
                     /translation="MVTQLINYALPLFMGLFLAIVLAAELGRRIGSWRQQGVGGPLPG
                     TAAVDAAIFALFGLLLAFAFSSAAARFDHRRDMIVAEANDIGTAYLRIDLA"
     gene            419..1273
                     /locus_tag="AC16A0_02040"
     CDS             419..1273
                     /locus_tag="AC16A0_02040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3104139.1"
                     /translation="MIDFPIIDTHVHLLDTQRFGYSWAKGAPKLGRDWTVADLTECAS
                     PYTIEGLVFVEVDVDMPQYLDEAVWVQSLADKEPRLKGCVACLPMERGAALEPEMAQL
                     AGLKVMRGVRRLIQNQPDPAFVLKPDFLAAVKLLPHYGLSFDICIFHHQLPNTLELVR
                     RCPEVSFILDHIAKPGIKAGLTDPWKKHVKELAEMPNVVCKLSGVTTEADHANWTREQ
                     LRPYIDHVIDCFGFDRIMYGGDWPVSELAGRYTDWIGVLDWATAGCTPAEKRKLFRDN
                     GMRAYRLS"
     gene            1294..>1794
                     /locus_tag="AC16A0_02045"
     CDS             1294..>1794
                     /locus_tag="AC16A0_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR3104140.1"
                     /translation="MTPELVARCHRDVPETKGHPAHDYFEDADYDRATAAVLAARPEG
                     PLWLFAYGSLLWRPEFEAIEARRAVASGWQRGFSMQILSHRGTPEQPGYMMCLDPGGE
                     CEGLALRLREDDTTAQIRILLYREIGSYEALDGVRWIEVKSSTGPLKALTFYARPDRL
                     PLYQPNR"
BASE COUNT          337 a          566 c          581 g          310 t
ORIGIN      
        1 gggccaagtc gatgcgaagg taagcggtgc cgatgtcatt ggcctccgcg acgatcatgt
       61 cccggcgatg gtcgaagcga gccgcagcgc tggagaaggc aaaggccagg agcaaaccga
      121 agagcgcaaa gatggcggca tccaccgcag ccgtaccggg caagggtccg cccacgccct
      181 gctgacgcca gctgccgatc cgccgcccca gttcggcggc aaggacaatg gccaggaaca
      241 atcccatgaa gagcggcaag gcatagttga tgagctgcgt aaccacgggt ctgctccagt
      301 cgcccaggat tcataatcga ttccatgatg cgatgcacca gaaggatgac ctggccccct
      361 cgccaagcgc ggtcaggggg cgctactatg gttcattagc cttctgcccg gagcaatcat
      421 gatcgacttc ccaatcatcg atacacacgt tcacctgctc gacacgcagc gcttcggtta
      481 ctcctgggcc aagggcgcgc ccaagctggg gcgagactgg acggtcgccg atctcaccga
      541 gtgtgccagt ccctacacga tcgaggggct cgtctttgtc gaggtcgacg tggatatgcc
      601 gcagtatctt gacgaggccg tctgggtgca gtcgctggcc gacaaggagc cccggctcaa
      661 gggctgcgtg gcctgcctgc ccatggaacg cggcgcggcg ctggagccgg agatggcgca
      721 acttgccggt ctcaaggtga tgcggggcgt gcgccgcctt atccagaacc agccggaccc
      781 cgcattcgtg ctgaagccgg acttcctggc agcggtcaag ctgttgccgc actacgggct
      841 gtccttcgac atctgcattt tccatcacca gcttccgaac acgctggaac tggtgcgccg
      901 ctgtcccgag gtgtcgttca ttctcgatca catcgccaag ccgggcatca aggccggcct
      961 gaccgatccg tggaagaagc acgtcaagga actggcggag atgccgaatg tcgtctgcaa
     1021 gctctcgggc gtgaccaccg aagccgacca cgcgaactgg acgcgcgagc agctgcgccc
     1081 ctacattgat catgtcattg actgctttgg ctttgaccgg atcatgtatg gcggcgactg
     1141 gccggtctcc gaactcgccg gtcgctatac cgactggatc ggcgttctcg actgggccac
     1201 ggcaggttgc acgccggcgg agaagcggaa actgttccgc gacaacggca tgcgggccta
     1261 tcgtctgagc tagccgtcgt gcgggtgatg cgcttgaccc ccgagctcgt ggcccgctgt
     1321 caccgggacg tgcccgagac gaagggtcac ccggcccatg actatttcga ggatgcggac
     1381 tacgaccggg caacagcagc cgtgctggcg gcaaggccgg aaggtccgct ctggctcttt
     1441 gcctatgggt ctctgctgtg gagacccgag ttcgaagcca tcgaggcgcg ccgcgccgtg
     1501 gcgagcgggt ggcagcgcgg gttctcgatg caaatcctca gccatcgcgg cacgcccgag
     1561 cagccgggct acatgatgtg cctcgatccg gggggagaat gcgagggtct tgcgctgcgc
     1621 ctgcgcgagg acgacacaac ggcgcagatc cggattctgc tctatcgcga gatcggcagt
     1681 tacgaggcgc tggacggcgt gcgctggatc gaggtgaaga gcagcaccgg accgctcaag
     1741 gcccttacgt tctacgcacg acccgaccgg ttgcctctct atcagcccaa ccgg
//
LOCUS       DBNGVR010000093         2418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1212737, whole genome shotgun sequence.
ACCESSION   DBNGVR010000093 DBNGVR010000000
VERSION     DBNGVR010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1212737"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="AC16A0_02050"
     CDS             complement(<1..1419)
                     /locus_tag="AC16A0_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3104141.1"
                     /translation="MTRDPRYDLLFEPVRIGPVTARNRFYQVPHCNGMGYRDASALAA
                     MRGMKAEGGWAVVCTEMVEIHHGADVSPYVELRIWSDQDIPMLARIAEKIHAHDSLAG
                     IELCHSGYTAVNLWSREIPMAASPMPAASFFNSPVQAREMDKEDIRNLRRAHRAAALR
                     ARAAGFDLVYVYAGHGLGIFQQFLSRATNFRSDEYGGSLENRARFLREVIEDTKDAVG
                     GSCAVPVRIAMNEFQGSAGLETAEVEDAIAMMAELPDLWDLSLSSWSKDSQTSRFAEE
                     GFQEPYIKGVKKLTSKPVVGVGRYTSPDSMVRVIRQGIMDMIGAARPSIADPFLPKKI
                     EDGRLDDIRECIGCNICITNDFTMTPLRCTQNPTMGEEWRRGWHPERIRPKRKDAHVL
                     VVGAGPAGLEAARALGQRGLTVTLADRASEAGGRVARECRLPGLAAWGRVRDWRLLQI
                     GKMPNVSLYLESDMTAETVAELG"
     gene            1503..>2418
                     /locus_tag="AC16A0_02055"
     CDS             1503..>2418
                     /locus_tag="AC16A0_02055"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3104142.1"
                     /translation="MSVAFADIEAAAERLGGRASRTPLLESAVLNATVGGRVLLKPEC
                     LQRTGSFKFRGAWNAISQLDPARTPGGVVAYSSGNHAQGVAAAARIVGLPALIIMPSD
                     TPRIKQENTRSYGAEVVLYDRATEDREAIAAYHVAHRSAVFIPPFDHPDVIAGQGTVG
                     LELAGQAVAAGCALDHVLVCCGGGGLVAGIAIALARLSPSTLVHSVEPDGFDDYARSL
                     RSGRRERNAAQAGSICDALLTPQPGVLTFEINRHLLGEGLVVDNRNVADAMRFAFEVL
                     KLVIEPGGAVALAAVLSGKLPTRGRNIGV"
BASE COUNT          408 a          792 c          784 g          434 t
ORIGIN      
        1 accgagttcg gccaccgtct cggctgtcat gtcgctttca agataaagcg agacattcgg
       61 catcttgccg atctgcagca gccgccagtc acgaacccgg ccccaggccg cgagacctgg
      121 cagccggcac tcgcgggcca cgcggccacc cgcttcggac gcacggtcgg cgagcgtgac
      181 cgtcagcccg cgttgaccaa gggcacgcgc cgcctcgaga cccgcaggac ctgcgccgac
      241 cacgaggaca tgtgcatcct tccgcttcgg acgaatccgt tccggatgcc agccacgccg
      301 ccactcctcg cccatggtgg gattctgcgt gcagcgcagc ggcgtcatgg tgaaatcgtt
      361 ggtgatgcag atgttgcagc cgatacattc ccggatgtcg tcgagacggc cgtcctcgat
      421 ttttttcggg agaaatggat cggcaatcga aggccgtgcg gcgccgatca tgtccatgat
      481 gccctggcgg ataacgcgca ccatggaatc gggcgatgtg tagcggccga cgccgacgac
      541 cggcttgctg gtgagtttct tcacgccctt gatgtatggc tcctggaagc cctcctcggc
      601 gaagcgcgag gtctgcgaat ccttggacca gctcgaaagc gacaggtccc agaggtcggg
      661 aagctcggcc atcattgcta tggcatcttc cacttcggcg gtttcgaggc ctgccgaacc
      721 ctggaactcg ttcattgcaa tgcgcactgg cacggcgcag ctgccgccca ccgcatcctt
      781 cgtgtcctcg atcacctcgc gcaggaaccg cgcacggttt tcaagcgagc caccatactc
      841 gtcgctgcgg aaattggtgg cccgcgagag gaactgctgg aagatcccca ggccatgccc
      901 ggcatagaca tagacaagat cgaagcccgc cgccctggca cgcagcgcgg ccgcgcggtg
      961 ggcgcggcgc aggttgcgga tgtcctcctt gtccatctcg cgggcctgca ccggactgtt
     1021 gaagaagctt gcggcgggca tggggctcgc cgccatgggg atctcgcgcg accagaggtt
     1081 cacggcggtg tatccggagt ggcagagctc gatgcccgca agcgagtcat gtgcgtgaat
     1141 cttctcggca atgcgggcca gcatcggaat gtcctgatcg ctccagatgc ggagttcgac
     1201 atagggtgag acatcggcgc cgtgatggat ctccaccatc tccgtgcaga ccaccgccca
     1261 gccgccctcg gccttcatgc cgcgcatggc cgccagcgcc gaggcgtccc gatagcccat
     1321 gccgttgcag tgcggaacct ggtagaagcg gttccgtgcc gtcacagggc cgatgcgcac
     1381 aggctcgaag agcaagtcgt agcggggatc tcgggtcatg gcagcctccg gtttcgcatg
     1441 tctctagcag gaaggcccgg ctcccggcta tggtcgcaag aactgctgcg tagggatcgc
     1501 gggtgagcgt tgcatttgca gacatcgaag cggctgcgga acgtcttggc ggccgcgcct
     1561 cacggacgcc gctgctcgaa tcggcggtcc tcaatgccac cgtcggcggg cgggtgctgt
     1621 tgaaacccga gtgcctgcaa cgcaccggct ccttcaagtt ccgcggcgcc tggaatgcca
     1681 tttcgcagct cgacccggcg cgaactcctg ggggagtcgt ggcctattcc tccggcaacc
     1741 atgcccaggg cgttgccgct gcggcgcgta ttgtcggcct tcctgccctg atcatcatgc
     1801 cgtccgacac gccacggatc aaacaggaga acacccgcag ctatggtgcc gaggtggtcc
     1861 tctacgaccg tgcaaccgaa gaccgggagg ccattgccgc ctatcacgtg gcacatcgca
     1921 gcgctgtctt cattccgccc ttcgatcatc ccgacgtcat cgcagggcaa gggacagtgg
     1981 gtctggaact ggcggggcag gcggttgcgg ccggctgcgc actggatcac gttctggtct
     2041 gctgcggtgg tggaggcctg gtggcgggca tcgcgatcgc cttggcccgg ctctcgccca
     2101 gcacccttgt gcacagcgtt gagccagacg gctttgacga ctatgcgaga agcctccgat
     2161 cggggcggcg cgaacgcaat gcggcccagg cgggctcgat ctgcgacgcg ctcttgacgc
     2221 ctcaaccggg cgtgctgacc ttcgagatca accggcacct cctgggtgag ggcctggtgg
     2281 tcgacaaccg caacgtggcc gatgccatgc gctttgcttt cgaagtgctg aagctggtga
     2341 tcgagcccgg cggcgccgtg gccctcgcgg ccgtcctgtc tggaaagctg ccgacacgcg
     2401 gccgcaacat cggcgtga
//
LOCUS       DBNGVR010000094         8816 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4944266, whole genome shotgun sequence.
ACCESSION   DBNGVR010000094 DBNGVR010000000
VERSION     DBNGVR010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4944266"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="AC16A0_02060"
     CDS             complement(<1..1232)
                     /locus_tag="AC16A0_02060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3104143.1"
                     /translation="MSRLNIAVIGSGISGLSAAWLLSQRHAVTLIERENRVGGHSNTV
                     DCPMPDGDVAVDTGFIVYNEPCYPNLTALFAHLKVPTAPSNMSFAVSMGGGAYEYAGS
                     TVLQMVGHARNLFSLGHMRMVRDLLRFLRSAAGRLDQIPDSLTLGDFIRQEGYSQDFA
                     DRHLLPMAGAIWSSASRSMADYPARAFIRFFDNHGLLKINDRPAWRTVRGGSQAYVSR
                     LVADGRFAVVTGGGAARIHRSLSGVTIALRDGSALDFDQVVIAAHADQSLRLLDQPTA
                     EENRILGGFSYSRNRTYLHTDAGLMPKRRRLWSSWNYLAPLTTGAESSSTVSYWMNSL
                     QPLPTTHDIFVTLNPQRLPREETIRAEFMYEHPVFNPRAMALQKELWTLQGANRTWYC
                     GAYFGAGFHEDGLQAGLAV"
     gene            1370..1957
                     /locus_tag="AC16A0_02065"
     CDS             1370..1957
                     /locus_tag="AC16A0_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3104144.1"
                     /translation="MTTAEQHSKQDKVILEAEFRNLMKRVAQNRDRAAFEKLFTHFAP
                     RLKSFMMRKNANAELAEDLVQEAMIAVWNKAGLYEPGKGSVTTWIFTIARNLRIDRIR
                     REAPMPLVELGDYDEASDDPPGDEVLARKQEDFLVSKALGGIPEEQRQILLLSFVEDM
                     AQSEIATRLNIPLGTVKSRMRLAYGHLRRILEIQP"
     gene            1954..2607
                     /locus_tag="AC16A0_02070"
     CDS             1954..2607
                     /locus_tag="AC16A0_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HIR3104145.1"
                     /translation="MTPAHHLDHSTILAYAAGTLDESIGLVVASHLAWCPQCRAAVRA
                     AEALGGELMEQLPDAAVSDGCRARTMAMLDGATLHRFPAPPVADAAVPGPLARLLPVA
                     NLDELPWKKKAPGVAIYDVKLSPTAKGQVKLLRIGPGRAVPEHGHGGEEVTLILSGAY
                     RDHMGRFGRGDVADLDEDIEHKPVAEAGGDCICLVAIERPTRFKSFAARLMQPLVGI"
     gene            2610..3359
                     /locus_tag="AC16A0_02075"
     CDS             2610..3359
                     /locus_tag="AC16A0_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104146.1"
                     /translation="MTASLPWTTAWITGASTGIGREVALQMAEAGVRVAASSRSAASL
                     EGLHPNIRPYPLDVTDAAAVAETVARIEAELGAIDLAVFAAAVYAPVDPARLDLKLFE
                     DALQTNVMGVVNCIAAALPKMQARGKGHLAWFASVAGYRGLPKAAAYGPSKAALINLA
                     ESLKPDLSRMGVAVSVINPGFVETPLTSKNDFPMPFLMKPADAARLTIQGLARGRFEI
                     SYPWQFVTMLKIARLLPYGLYFRMIDRMVFK"
     gene            complement(3362..4030)
                     /gene="ccmB"
                     /locus_tag="AC16A0_02080"
     CDS             complement(3362..4030)
                     /gene="ccmB"
                     /locus_tag="AC16A0_02080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR3104147.1"
                     /translation="MKLLWALIRRDIVLTLRQGGGAGTALGFFLTVVVLLPIGLGPNQ
                     ALMQKLAPGALWIALLLSVLLSADRIFQQDYDDGSLDIMAMGPVPFELVALAKSVAHW
                     LSTSLPLAIAAPFLGFLLNLSPSVILPLFLAMTLGSITLSLLAAIGAAVTVGLRRGGL
                     LVSLLVLPLYVPVLIFGVAASNGGVMGDGVAPAALLILAAIALASLVLSPIAAAAALR
                     SYLK"
     gene            complement(4027..4626)
                     /gene="ccmA"
                     /locus_tag="AC16A0_02085"
     CDS             complement(4027..4626)
                     /gene="ccmA"
                     /locus_tag="AC16A0_02085"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR3104148.1"
                     /translation="MKLTADNLTCERGGRIVFAGIGFSLGEGQLMQLTGPNGAGKSSL
                     LRLIAGLNEAAAGTLVLAGGDSDLTIGQQAHYIAHQEAVKTALTVEENLAFWRDFLGG
                     GSLEAALEAFALSRFASFQAGLLSAGQKRRLALARLALITRRLWLLDEPTVGLDTASL
                     ARLVAVMSAHLDHGGMIIAATHVPLGRDPDLRLDLEGRT"
     gene            complement(4694..5419)
                     /gene="phbB"
                     /locus_tag="AC16A0_02090"
     CDS             complement(4694..5419)
                     /gene="phbB"
                     /locus_tag="AC16A0_02090"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIR3104149.1"
                     /translation="MGRVAVVTGGSRGIGAAISKALKAAGYKVAANYAGNDGAAAKFK
                     AETGIPVYKWDVSNYEACAAGLKQVEADLGPVEVLVNNAGITRDAMFHKMTPQQWNEV
                     INTNLNSLFNMTQPVWGGMRDRSFGRVICISSINGQKGQMGQANYSAAKAGDIGFVKA
                     LAQEGAAKGITVNTICPGYIATEMVKAIAQDVLEKRILPLIPVGRLGEPEEIARAVVF
                     LASDDAGFITGSTLSANGGQYMM"
     gene            complement(5447..6631)
                     /locus_tag="AC16A0_02095"
     CDS             complement(5447..6631)
                     /locus_tag="AC16A0_02095"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR3104150.1"
                     /translation="MAGSENGVVIVSAARTPVGSFNGSLSALSAAELGTVAIKAVMER
                     AKVEPGEVEEVIMGQVLAAGQGQNPARQAAVHSGIPHEAPAWGMNQLCGSGLRAVALG
                     LQQIVLGDSRIVVAGGMESMSRAPHVAHLRDGVKMGDHHLIDTMIKDGLWDAFNGYHM
                     GTTAENVARQWQISRDMQDAFAVASQNKAEAAQAAGRFKDEIAPVTVSTRKGDVVVSE
                     DEYIKKGVTRESVGKLRPAFAKDGSVTAANASGINDGAAAVLLMSARDAEKRGLTPLA
                     RIASWAHAGVDPAIMGTGPIPASRLALKKAGWKVSDLDLVEANEAFAAQACAVNKDMG
                     WNPDIVNTNGGAIAIGHPIGASGARVLNTLLFEMQRRNAKKGLATLCIGGGMGVAMCV
                     ER"
     gene            6828..7430
                     /gene="phaR"
                     /locus_tag="AC16A0_02100"
     CDS             6828..7430
                     /gene="phaR"
                     /locus_tag="AC16A0_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HIR3104151.1"
                     /translation="MTEEKAAPQNAVVIKKYANRRLYNTATSTYVTLDDLASMVKGGT
                     DFLVYDAKSGEDITRSVLTQIIFEEENKGTNLLPINFLRQLIRFYGDSMQAFVPGFLE
                     FSLENLGKEQEKFRSQLMEAWGADPFKAMQDHAQRNMAMFNDAMKMFNPFAAMGGMPG
                     ASGTAAQPPRTGPAPARDDLQVLKDQLAAMQQKIDTLAQK"
     gene            complement(7434..>8816)
                     /locus_tag="AC16A0_02105"
     CDS             complement(7434..>8816)
                     /locus_tag="AC16A0_02105"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3104152.1"
                     /translation="NNCDEEHLTIAAERFLSVARESVIDTQSGPVWALLSIGALILPT
                     HAEDPNTAMARAEEALAEARRRPSDGYVVYRPSPQRSSERALNARSATETVRCLREGL
                     FRLAFQPVIAAKTRTPVFHEALLRMVDPAGEVITAVHLIPIAEKLGLVRLIDRAVAQM
                     VVASLHKYSDARLSLNLSGTTATDPRWYPQIIEMLASNRAVTDRLTIEITETVALGDL
                     HETVRFVEQLRELGCRVAIDDFGAGFTSFRNLRALPIDMLKLDGTFCRDLAGNGDNTY
                     FVRSLIDMARTFNIQTVAEWVESEEDAVLLRDWGIDLMQGNLFGEAILQAPWPAAKEA
                     AAAGARSPFVLPSEIEWDSALAETPVLREEDLASLPAEPAEAAAPWNPVSEPVASVTD
                     SGTSADSPEDGATKTVVQSFEHALSGELSKLRDAISALDQALKGDRSAKAAEPSFADL
                     VSDTARRATG"
BASE COUNT         1593 a         2804 c         2910 g         1509 t
ORIGIN      
        1 acggcaaggc cggcctgcag tccgtcctcg tggaagcccg cgccgaaata ggcgccgcaa
       61 taccaggtgc ggttcgcacc ctggagcgtc cacagttcct tctgcagtgc catggcacgc
      121 ggattgaaga ccggatgttc gtacatgaat tccgccctga tggtctcctc ccgcggcaat
      181 cgctgcgggt tcagggtgac gaagatatca tgcgtggtcg gcaaaggctg cagcgaattc
      241 atccagtagc taacggtgct ggatgactcc gcgccggttg tgagcggtgc caggtagttc
      301 cagctggacc aaagccggcg gcgtttcggc atgaggccgg cgtcggtgtg aagataggtg
      361 cggttacgcg agtaggaaaa gccgccgagg atgcggtttt cctcggccgt cggctggtca
      421 agcaggcgaa gggactggtc ggcgtgcgcg gcgatcacca cctggtcaaa atcgagcgca
      481 ctgccgtcgc gcagcgcaat ggtcacgccg gacaggctgc ggtgaatgcg tgcagcgcct
      541 ccgcccgtca ccacggcgaa gcgtccgtcg gcgacgaggc gcgagacata ggcctgcgag
      601 ccgcccctga cggtgcgcca ggccgggcga tcgttgatct tgagcaaccc gtgattgtcg
      661 aagaaacgga tgaaggcccg ggcgggatag tcggccatgc tgcgcgaggc cgaggaccag
      721 atggcgcctg ccatgggcag gagatggcgg tcggcgaaat cctgggaata gccttcctgg
      781 cggatgaaat cgccgagcgt cagggagtcg gggatctggt cgagccgtcc ggccgccgac
      841 ctgagaaagc gcagcagatc gcgcaccatg cgcatgtgcc cgaggctgaa aaggttgcgg
      901 gcgtggccga ccatctgcag gacggtggac cccgcatatt catatgcccc gccgcccatg
      961 ctgacggcga aactcatgtt ggacggggcc gtaggtacct tcaggtgggc aaagagagcg
     1021 gtcagattgg gatagcaggg ctcgttgtag acgatgaagc ctgtgtccac cgccacgtcg
     1081 ccgtccggca tcgggcaatc gaccgtattg gaatggccac cgacgcggtt ctcgcgttcg
     1141 atcagggtaa cggcgtgacg ctgcgacagc agccaggccg ccgacaggcc ggaaatgccc
     1201 gacccgatga cggcaatatt cagtctggac aaaagaaccc cgagcctttt ttagaacgtt
     1261 gaagatatta cgggcgcttc cccgggttgg atcaagccgg tgatccgtcc ccgggaagcg
     1321 cccgtaattg caccagagat gaggaaacaa tgttatgctt gtagctgcca tgacaacggc
     1381 tgaacagcac agcaagcagg acaaggtcat ccttgaagcc gagttccgca acctgatgaa
     1441 acgggtggcg cagaaccggg acagggcggc attcgagaag cttttcacgc atttcgcgcc
     1501 ccggttgaaa agcttcatga tgcgcaagaa cgcgaatgcc gaactggctg aggatctggt
     1561 ccaggaggcg atgatcgcgg tgtggaacaa ggctggtctc tacgagcccg gaaagggttc
     1621 ggttaccacc tggatcttca ccatcgcccg caacctgcgc atcgaccgta tccggcgcga
     1681 ggcccccatg cctctcgtcg aactgggcga ctatgacgaa gcctcggacg atccgccggg
     1741 cgacgaagtt ctcgcccgca agcaggagga tttcctggtg agcaaggcac tgggcggcat
     1801 tcccgaagaa cagaggcaga tccttctgct ctccttcgtc gaggacatgg cccagagcga
     1861 gatcgccaca aggctcaaca tcccgcttgg aaccgtcaaa tcgcgcatgc gtctggccta
     1921 tggccacttg cgccgcatcc tggagataca accgtgacgc ccgcccatca cctcgaccac
     1981 agcaccatcc tggcctatgc ggccggcacc ctcgacgagt cgatcggcct ggtcgtcgca
     2041 tcgcacctgg cctggtgccc gcagtgccgg gcagccgttc gcgcggccga ggcgctgggt
     2101 ggggaactga tggaacagct gccggatgcc gctgtgtcgg acggctgccg ggcccgcacc
     2161 atggccatgc tggatggcgc cacgctgcac cgtttcccgg caccgccggt cgctgatgcc
     2221 gccgtgcccg gtcccctggc gcgccttttg ccggtggcca atctcgatga gctgccgtgg
     2281 aagaagaagg cgccgggcgt ggccatctat gacgtgaagc tctcgcccac cgcaaaggga
     2341 caggtgaagc tcctgcgcat cggcccgggc cgcgccgtcc cggaacacgg ccatggcggc
     2401 gaggaggtaa cgctcattct cagcggcgcc tatcgcgatc acatgggtcg ctttggccgc
     2461 ggcgatgtgg ccgatctcga cgaggacatc gagcacaagc ccgtggccga ggccggcggg
     2521 gattgcatct gcctggtcgc catcgagcgg ccgacgcgct tcaagtcctt cgccgcccgc
     2581 ctgatgcagc cgctcgtcgg catctgaccg tgacggcatc cctgccgtgg acaacggcgt
     2641 ggatcaccgg tgcctcgacc gggatcgggc gggaagtggc gttgcagatg gcggaagccg
     2701 gcgtgcgcgt agccgcctcc tcgcgcagcg ctgccagcct tgaggggctg catcccaaca
     2761 ttcgtcccta tccgctcgat gtgaccgatg cagccgccgt cgccgagacg gtggcgcgca
     2821 tcgaagccga gcttggcgcc atcgacctcg cggtctttgc ggctgccgtc tatgcgccgg
     2881 tcgatcccgc ccggctggac ctcaagctgt tcgaggatgc gctgcagacc aacgtgatgg
     2941 gcgtggtcaa ctgcatcgct gcggcgctgc cgaagatgca ggcgcgcggc aagggccatc
     3001 tcgcatggtt cgcctcggtg gccggctatc gcggccttcc caaggccgcg gcctatggtc
     3061 cctccaaggc ggcgctgatc aatctggccg aaagcctgaa gccggatctt tcccgcatgg
     3121 gagttgcggt gagcgtcatc aatccgggat tcgtcgaaac gccgctcacc tcgaagaatg
     3181 actttcccat gcccttcctg atgaagcctg ccgatgcggc gcggctcacc atccagggcc
     3241 tggcgcgcgg ccgtttcgag atcagctatc cctggcagtt cgtgaccatg ctgaagatcg
     3301 cgcggctgct gccctatggt ctttacttcc gcatgatcga ccggatggtc ttcaagtagg
     3361 cttacttgag ataggagcgc agcgccgccg cggccgcgat cggcgacagc accaggctgg
     3421 ccagtgcgat ggccgcgagt atcagcaagg cagccggcgc cacgccatcg cccatcacgc
     3481 cgccgttgga agccgcgact ccgaagatca gcaccggcac atagagcggc agcaccagca
     3541 gcgagaccag caacccgccg cggcgcagtc ccaccgtgac ggccgcgccg atcgccgcca
     3601 gcagcgatag cgtgatcgag cccagcgtca tggcaaggaa gagcggcagg atgacggatg
     3661 gcgagagatt gaggaggaaa cccaggaagg gcgcggcgat ggccagcggc aggctggtgg
     3721 acagccagtg ggcgacggac ttggccagtg ccacgagttc gaagggcacc gggcccatgg
     3781 ccatgatgtc gagcgagccg tcgtcgtagt cctgctggaa gatccggtcg gccgagagca
     3841 gcaccgaaag cagcagcgcg atccacagcg cgccgggggc aagcttctgc atcagcgcct
     3901 ggttgggacc gaggccaatg ggcaggagca ccacgacggt cagaaagaat ccgagcgccg
     3961 tgccggcacc gccgccctgg cggagggtga ggacgatgtc gcggcggatc agcgcccaga
     4021 gcagcttcat gtccggccct cgaggtcaag gcggagatcg ggatctcggc cgagcggaac
     4081 atgtgtggcc gcgatgatca tgccgccgtg gtcgagatgc gccgacatga cagcgacgag
     4141 cctggcaagc gaggccgtgt caagtcccac cgtgggttca tcgagaagcc agagccggcg
     4201 cgtgatcagc gcgaggcgcg ccagcgcgag tcgacgcttc tgcccggctg agagcagtcc
     4261 ggcctgaaag gatgcgaaac gtgacagcgc aaaggcctcg agggcggcct cgaggctgcc
     4321 gccgccaagg aaatcgcgcc agaaggcgag gttctcctcg acggtgagcg ccgtcttcac
     4381 cgcctcctga tgcgcgatgt aatgggcctg ctggccaatg gtgagatcac tgtcgccgcc
     4441 tgcaagcacg agcgttcccg ctgcggcctc gttcagcccg gcaatgaggc ggagcagtga
     4501 cgacttgccc gcgccattgg gcccggtcag ctgcatgagc tgtccttcgc caagcgagaa
     4561 gccgatgcct gcgaagacga tgcgtccgcc gcgctcgcac gtcaggttgt ccgccgtgag
     4621 cttcatgccg gatagcgcct agcgattgac cgcaggaatg caacagcccg ccgcatgccg
     4681 atccggctcg cgctcacatc atgtactggc cgccattggc cgagagtgtc gaaccggtga
     4741 tgaagcccgc atcgtcggag gcgaggaaga ccaccgcgcg ggcgatctcc tcgggctcgc
     4801 cgaggcggcc gacaggaatg agcggcagga ttcgcttttc cagcacgtcc tgggcgatgg
     4861 ccttgaccat ttcggttgcg atgtagcccg ggcagatggt gttgacggtg atgcccttgg
     4921 ccgcgccctc ctgggcaagc gccttcacga agccgatgtc gccggccttc gccgcggagt
     4981 agttggcctg ccccatctgc cccttctggc cgttgatgga agagatgcag atgacgcggc
     5041 caaagcttcg gtcgcgcatg ccgccccaga ccggctgggt catgttgaag agtgaattca
     5101 ggttggtgtt gatcacctcg ttccactgct ggggggtcat cttgtggaac atggcgtcgc
     5161 gggtgatgcc ggcattgttg accagcacct cgaccggacc gagatcggcc tcgacctgct
     5221 tgaggccggc cgcacaggcc tcgtagttcg agacatccca cttgtagacc ggaatgccgg
     5281 tctctgcctt gaactttgcc gcggccccgt cattgccggc atagttggcc gccaccttgt
     5341 agcctgcggc cttcagcgcc ttcgagatcg ccgcgccaat gccgcggctt ccgcccgtca
     5401 caactgcaac ccgtcccatt tcacgtgtcc tcttctgatt ctggtgttag cgttcgacgc
     5461 acatggcgac gcccatgccg ccgccgatgc acagcgtggc aaggcccttc ttcgcattgc
     5521 ggcgctgcat ctcgaagagc agcgtgttga gtacgcgcgc gccggacgcg ccgatcgggt
     5581 ggccgatggc aatggcgccg ccgttggtgt tgacgatgtc ggggttccag cccatgtcct
     5641 tgttgaccgc acaggcctgg gcggcgaagg cctcgtttgc ctccaccaga tcgaggtccg
     5701 aaaccttcca gccggccttc ttgagcgcca gccgcgaggc gggaatggga cctgtgccca
     5761 tgatcgcggg atcgacgccg gcatgagccc aggacgcgat gcgggccagc ggcgtgaggc
     5821 cgcgcttctc ggcatccctg gcgctcatca gcagcacggc ggcggcgccg tcattaatgc
     5881 cggaggcgtt ggcggcagtg accgaaccgt ccttggcgaa ggccgggcgg agcttcccga
     5941 ccgactcgcg cgtgacaccc ttcttgatgt actcgtcctc gctcaccacc acatcgccct
     6001 tgcgggtgga gacggtcacg ggggcaatct cgtccttgaa cctgccggcg gcctgtgcag
     6061 cctcggcctt gttctgcgag gcgaccgcaa aggcatcctg catgtcgcgc gaaatctgcc
     6121 attgcctggc gacgttctcg gcggtcgtac ccatgtgata accgttgaag gcatcccaga
     6181 gcccgtcctt gatcatggtg tcgatcaggt gatggtcgcc catcttcacc ccgtcgcgca
     6241 gatgcgccac atgcggtgcg cggctcatcg attccatgcc gccggccacc acgatgcggg
     6301 aatcgcccag cacgatctgc tgcaggccga gcgccacggc gcggaggccc gagccgcaga
     6361 gctggttcat gccccaggcc ggggcctcat gcgggatgcc ggaatggacg gcggcctggc
     6421 gggccgggtt ctggccctgc ccggcggcaa gcacctgccc catgatcact tcctcgacct
     6481 cgcccggctc gaccttggca cgctccatca ccgccttgat cgccacggta ccgagttcgg
     6541 cggccgacag ggccgacagc gacccattga acgagcccac cggcgtgcgc gccgcggata
     6601 cgatgacaac cccattttca gaaccggcca tgaactgcct ccttgttgga ctttgcggtc
     6661 aattgcatat gccctccata gcattgtttg tgcaatgcga cataggttgt gggcgggtcc
     6721 cgccgaaatt ttcgcgcttg caattgtgca gtgcggggag caagtttttg cggtggaccc
     6781 ctgcgaaaag ccggaaaacc ggggccattg ctggaggagt aagggcgttg accgaggaaa
     6841 aagctgcacc gcaaaacgct gtcgtcatca agaaatacgc caaccggcgg ctctacaaca
     6901 cggcaacgag cacctatgtg acgctcgacg atctggcttc catggtcaag ggtggaaccg
     6961 atttcctcgt ctacgacgca aaatcgggcg aggacatcac ccgttcggtg ctgacccaga
     7021 tcatcttcga agaggaaaac aagggcacca acctgctgcc gatcaatttc ctgcgccagc
     7081 tcatccgctt ctatggcgac tcgatgcagg ccttcgtacc cgggtttctg gagttctcgc
     7141 tggagaatct cggcaaggag caggagaagt tccggagcca gctgatggaa gcctggggag
     7201 ccgatccctt caaggccatg caggatcatg cccagcgcaa catggcgatg ttcaatgatg
     7261 ccatgaagat gttcaatccc ttcgctgcca tgggtggcat gcccggcgct tccggaaccg
     7321 cggcccagcc gccccggacc ggccccgcgc cggcccgcga cgacctgcag gtcctcaagg
     7381 accagcttgc cgcgatgcag cagaagatcg acacgctcgc ccagaagtag gcgtcagccg
     7441 gtagcgcgcc gggcagtgtc gctgaccagg tcggcgaagc tcggttctgc agccttggca
     7501 gacctgtctc ccttcagcgc ctgatcgagc gccgaaatcg catcccggag cttgctcagt
     7561 tcgcccgaca gcgcgtgctc gaaggattgc accaccgtct tcgtggcgcc gtcctccgga
     7621 ctgtcggccg aggtgccgga atccgtgacg gaggctaccg gctcgctgac cgggttccac
     7681 ggtgcggcgg cctctgcagg ttcggccggc agggacgcga gatcctcttc gcgcaggacc
     7741 ggcgtctcgg cgagcgcgct gtcccactcg atctccgaag gcagcacgaa tggcgaacgg
     7801 gccccggccg ctgccgcttc cttcgctgca ggccagggag cctgcaaaat ggcttcgcca
     7861 aagagattgc cctgcatgag gtcaatcccc cagtcgcgca gcagcacggc atcctcttcg
     7921 gactcgaccc attcggcgac cgtctggata ttgaaggtac gggccatgtc gatcagtgag
     7981 cggacgaaat aggtgttgtc gccgtttccg gcgaggtcgc ggcagaaggt gccgtcgagc
     8041 ttgagcatgt cgatcggcag ggcgcgcaga ttgcggaaag aggtgaagcc ggcgccgaag
     8101 tcgtcgatgg cgacgcggca gcccagttcg cgcagctgtt cgacgaagcg cacggtctca
     8161 tggagatcgc caagcgcgac ggtttcggtg atttcgatgg tgagcctgtc ggtaacggca
     8221 cgattggacg ccagcatctc gatgatctgg ggataccagc gcggatcggt tgccgtcgtc
     8281 ccggagagat tgagggacag ccgggcgtcg gaatacttgt gcagcgacgc caccaccatc
     8341 tgcgccacgg cacggtcaat gaggcggacc aggcccagct tctcggcgat cggaatgagg
     8401 tgtacggcgg tgatcacctc gccggccgga tcaaccatgc gcagcagcgc ctcgtggaac
     8461 accggcgtgc gggtctttgc cgcgatcacc ggttggaaag ccaggcggaa caggccctcg
     8521 cgcagacagc gtacggtttc ggtggcgctg cgggcattga gcgcgcgctc ggaactgcgc
     8581 tgtggcgacg gacggtagac cacgtaacca tccgacgggc ggcgcctcgc ctcggccagc
     8641 gcttcctcgg cccgggccat tgccgtgttc ggatcctcgg catgggtggg caggatcagg
     8701 gcgccgatgg acagcagcgc ccagaccggt ccgctttggg tatcgatgac gctttcgcgg
     8761 gccacactga gaaaacgctc ggcggcgatg gtgagatgtt cctcgtcgca attgtt
//
LOCUS       DBNGVR010000095         5237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_427665, whole genome shotgun sequence.
ACCESSION   DBNGVR010000095 DBNGVR010000000
VERSION     DBNGVR010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_427665"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC16A0_02110"
     CDS             complement(<1..454)
                     /locus_tag="AC16A0_02110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIR3104153.1"
                     /translation="MSGAKEKGQPAAVAYCFDRHFAPYAAVSTLSLLASSKTPVAIYW
                     CVDQRDTQHLAPYIEAIGKKTAANIQVVPVDVGEFLAWKTVHHFTPATYLRLLLPEVL
                     PLDKLIYIDADTLVLGDLSDLFAVDLGERVLAGVPDLAGARVSKIGRTA"
     gene            complement(544..1719)
                     /locus_tag="AC16A0_02115"
     CDS             complement(544..1719)
                     /locus_tag="AC16A0_02115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3104154.1"
                     /translation="MIRPPVPAAPGATLSLLALGNFVVGMGAFVVIGIVTPIAESFGV
                     PKSSAALVLTAYAISYALLSPIAAAVTGTIARRYVLSGALAVFAVGSILSALAPSLEL
                     LTASRVLVAMGGALYTPMSAGVAVAIMPPEQRGRALAKVFGGITLAHVVGVPFGAWIT
                     YRFDWPATFWTVVVLAAAVSALLYSRIPAAVNFQPSRLATIVSALKDLRLVVAVAFTA
                     TIITAIYIVFTYFGPIIEASIGTNPEVRTGFLVLYGLGAVAGNFMGGYLTDRIGPGRT
                     LVAVCLAQAAIMPFLSIHPWSPVPFAVLVGLWSAFGWSFMAPQQTRLTTIAPEAVSLA
                     LALNAAMIYLGIAIGSALAALVIRTHGLGGLGIAGGLMALIALVHLWLSFRLSSRAI"
     gene            complement(1730..2146)
                     /locus_tag="AC16A0_02120"
     CDS             complement(1730..2146)
                     /locus_tag="AC16A0_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3104155.1"
                     /translation="MLSSSRFIVAVHALSVLARFSGRGPVCSAMVAESVHTNPVVIRR
                     LMGELEKAKLVRSVAGRSGGFELDRQADQITLADVYFAVEDENVFRMHKADPNSRCPV
                     AAQLGKVLHGPLRAAECALHTSLAKTTLRDVSTAIV"
     gene            2272..2562
                     /locus_tag="AC16A0_02125"
     CDS             2272..2562
                     /locus_tag="AC16A0_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR3104156.1"
                     /translation="MRRFDLAVTFVCCALLGYFAWHGWKGPRGFPYQERLQAKMEVLT
                     GKQAEVVTSRRQLEEKVALLRPESIHPDLLDEMARGQLQMARPGDLVVYTNR"
     gene            2698..3714
                     /gene="pdhA"
                     /locus_tag="AC16A0_02130"
     CDS             2698..3714
                     /gene="pdhA"
                     /locus_tag="AC16A0_02130"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR3104157.1"
                     /translation="MSRKGGKAEKTDLKNTGFSAEQEQRAYRDMLLIRRFEEKAGQLY
                     GMGFIGGFCHLYIGQEAVVVGLQMAQKEGDQVITSYRDHGHMLACGMDPKGVMAELTG
                     RRGGYSKGKGGSMHMFSREKQFFGGHGIVGAQVPLGAGLAFANKYRGNDHVSVTYFGD
                     GAANQGQVYETFNMASIWKLPVIFVIENNRYAMGTAAPRSTALSEQLLKRGEAFGIPA
                     EQVNGMDVRAVKDAGDRAVEHCRAGHGPYILEMLTYRYRGHSMSDPAKYRSKEEVQKM
                     REENDPIEQVKARILKHGYASEDDLKKVDADIRAIVTEAAEFAQNDPEPDVSELFTDI
                     YVNA"
     gene            3726..4004
                     /locus_tag="AC16A0_02135"
     CDS             3726..4004
                     /locus_tag="AC16A0_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104158.1"
                     /translation="MLVAYAMPLILASAIGAWLVAVWSAISVVRLAPPGQKVRAYFNL
                     GWWQFAKVRASIGPAAEPHIIRFRCAFYVFFAVIASVFLAVAGLLAFS"
     gene            4025..>5237
                     /locus_tag="AC16A0_02140"
     CDS             4025..>5237
                     /locus_tag="AC16A0_02140"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HIR3104159.1"
                     /translation="MPNILMPALSPTMEEGKIAKWLKNVGDMVKSGDVIAEIETDKAT
                     MEVEAVDEGPLSAILVPAGTENVKVNTPIAVIGVADAAPAPKPAAAATPAAAAPAAPA
                     PTFAGPDIAATPDENPEVPAGTEMVMMTVREAIREAMTEEMRRDKDVFLMGEEVGQYQ
                     GAYKISQGMLDEFGPQRVIDTPITEHGFTGIAVGAAFAGLKPIVEFMTFNFSMQAIDH
                     IINSAAKTLYMSGGQMGCPIVFRGPNGAAARVAAQHSQDFAAWYGHIPGLKVVAPYTA
                     ADAKGLMKAAIRDPNPVVFLENEILYGKSFEVPKLEDWVVPIGKARIARAGRHVTIVS
                     FGIGMAYALAAAEKLAADGIEAEVIDLRTIRPMDIATVVASVKKTNRCVAVEEGFPQF
                     SVTSEIAAQIMT"
BASE COUNT         1053 a         1613 c         1693 g          878 t
ORIGIN      
        1 cagccgttcg cccgatcttg ctgacgcgcg ctcccgccag atcaggaacg ccggcaagca
       61 cgcgctcacc aaggtcgacc gcgaaaagat cggagaggtc tccaagcaca agcgtatcgg
      121 catcgatata gatgagcttg tccagcggca gaacctcggg aagcaggagc cggaggtatg
      181 tggctggcgt gaagtgatga acggtcttcc aggcgagaaa ctcgcccaca tcgaccggga
      241 ccacttggat gttcgcggcc gtcttcttgc cgatggcctc gatgtacggg gccaggtgct
      301 gcgtgtccct ttggtcaaca caccaataga tcgctacggg cgtcttgctg ctcgcaagca
      361 agcttagcgt ggagacggcg gcataaggcg cgaagtgcct gtcgaagcag tatgctacag
      421 ccgcaggctg acctttctcc ttcgcgccgc tcacaggatc aacctctcga tgattcagat
      481 gacttcatct tggccatgat ctagccgctc ccacgccgca gtgccaccgg ccagcaactc
      541 attctagatg gcccggctcg aaagccggaa cgagagccag aggtgcacaa gggcaatgag
      601 ggccatcaat ccgccagcga tcccgagacc ccccaagcca tgcgtgcgga tcaccagggc
      661 cgccagcgcg gatccgatcg cgatgccgag atagatcatc gcggcattga gcgcgagcgc
      721 gagcgagact gcctccggtg cgatggtggt caaccgggtc tgctgcggcg ccatgaagga
      781 ccaaccgaag gcgctccaca ggccgaccag cactgcaaag gggaccgggc tccacggatg
      841 gatcgagagg aacggcatga tcgcggcctg agccaggcag actgccacaa gggtacggcc
      901 cggaccgatg cgatccgtca ggtatccgcc catgaagttt ccggcgaccg cacccaggcc
      961 ataaaggacc aggaagcccg tgcgcacttc ggggttggtg ccgatcgagg cctcaatgat
     1021 cgggccaaag taggtaaaga cgatgtagat ggccgtgatg atggttgcgg tgaatgctac
     1081 ggccacgacg aggcgtaagt ccttcaaggc cgagacgatc gtggccaagc gcgatggctg
     1141 gaagttcact gctgccggta tccggctgta gagcagagcc gacacggctg cggccagcac
     1201 gaccacggtc cagaaggttg caggccagtc aaaccggtag gttatccacg cgccgaaagg
     1261 gacgccgacc acgtgcgcca gggtaattcc gccaaaaacc ttggccaggg cgcggccccg
     1321 ttgctccggt ggcatgatgg cgacagccac gcccgctgac attggcgtgt agagcgctcc
     1381 gcccattgcc acgagcacgc gcgaggccgt gagaagttcg aggctgggcg ccagggcgga
     1441 gaggatcgaa ccgactgcga aaacggcaag cgcgcccgac aggacgtagc gccgggcgat
     1501 cgtgccggtg acggctgctg caatgggcga gagcagcgca taggaaatgg cataggccgt
     1561 cagcaccagc gcagcgctcg acttggggac accgaagctt tcggcgatcg gcgtaacgat
     1621 gccgatgacc acgaaggcgc ccatgccgac aacgaagttg ccgagggcca gaagactgag
     1681 cgtggctccg ggtgccgccg ggaccggtgg ccggatcatc ttccgaggat cagacgattg
     1741 ccgtcgacac gtcgcgcagc gttgtcttgg ccagcgaggt gtggagtgcg cactctgcag
     1801 cgcgcagggg cccgtgcaat acctttccga gctgcgctgc caccgggcag cgcgagttgg
     1861 gatcggcctt gtgcatgcgg aagacgttct cgtcctcgac cgcgaagtag acgtcggcca
     1921 gcgtgatctg gtcggcctgg cgatccagct cgaatcctcc ggagcgaccc gccacggacc
     1981 tcacgagctt ggccttctca agctcgccca tgagccggcg gatcacgacc ggattggtgt
     2041 ggacgctctc agcaaccatg gccgaacaca ccgggccccg gcccgaaaac cgggcgagga
     2101 cgctgagcgc gtggacggcg acaatgaatc ggctgctgga caacattttg gtaacccttg
     2161 cagttacatc ggcgcggccc tgccaaaggt caagagacta tatctcaaat cgttacccat
     2221 tgttaaccga gtcggcggaa atctgggtgc tgtttcggag cccttgccct catgcgtcgt
     2281 ttcgatcttg ccgtcacctt cgtctgctgt gccttgcttg gctatttcgc ctggcatggg
     2341 tggaaagggc cgcggggctt cccataccag gagcggctac aggccaagat ggaagtcctg
     2401 accgggaagc aggcggaggt cgtgacgagc cgccggcaac tggaggaaaa ggtggcgctg
     2461 ctgcggcccg aaagcatcca ccccgacctt ctggacgaga tggcacgcgg gcagctgcag
     2521 atggcgcggc caggcgacct cgtggtctac acgaaccgct gaattcccct gcgaaatcct
     2581 tgctgtaaac caaaatccgg ttaatctccg ggatcgcctg catttccagt ggtttaatgt
     2641 tatgttctcg ccatccagat cgatgcggag ggagccatgg ccgtgaaatc agcctacatg
     2701 tcgcgcaagg gtggcaaggc cgagaagacc gacctgaaaa acacgggctt cagcgccgaa
     2761 caagagcagc gcgcctatcg cgacatgctg ctgatccgcc gcttcgagga aaaggccgga
     2821 cagctctacg gcatggggtt catcggcggt ttctgtcacc tctatatcgg ccaggaggcc
     2881 gtggtcgtgg gccttcagat ggcccagaag gagggcgacc aggtcatcac atcctatcgc
     2941 gaccatggcc acatgctggc ctgcggcatg gatcccaaag gggtcatggc cgaactgacc
     3001 gggcgccgcg gcggttactc caagggcaag ggcggctcca tgcacatgtt cagccgcgag
     3061 aagcaattct tcggaggaca cggaatcgtc ggcgcgcagg tgccgctcgg ggcagggctt
     3121 gccttcgcca acaagtatcg tggcaacgac catgtctcgg tcacttactt cggcgacggc
     3181 gcggccaacc agggacaggt ctacgagacc ttcaacatgg cctccatctg gaagctgccg
     3241 gtgatcttcg tcatcgagaa caatcgctat gccatgggta cggcagctcc gcggtcgacg
     3301 gcgctctctg agcaattgct caagcgcggc gaggccttcg gcattccggc cgagcaggtg
     3361 aacgggatgg acgtgcgtgc ggtgaaggac gccggggacc gggccgtcga gcactgccgt
     3421 gccggccatg gcccctacat cctggagatg ctgacctatc gctatcgcgg gcactcgatg
     3481 tccgatccgg cgaagtatcg ttcgaaggaa gaagttcaga agatgcgcga ggagaacgat
     3541 ccgatcgaac aggtcaaggc gcgcatcctc aagcacggct atgccagcga agacgacctc
     3601 aagaaggtcg atgccgacat ccgggccatc gtgaccgagg cggccgaatt cgcccagaac
     3661 gatccggagc cggacgtgag cgagctcttc accgacatct atgtgaacgc ctgagcggct
     3721 gcgccgtgct ggtcgcctat gccatgcccc tgatcctggc cagtgccatc ggcgcctggc
     3781 tggtggccgt gtggtcggcc atctcggtcg tcaggctggc gccgcccggg cagaaggtgc
     3841 gggcctactt caatctcggc tggtggcagt tcgccaaggt gcgcgccagc atcggcccgg
     3901 cggccgagcc ccacatcatc cgcttccgct gcgccttcta tgtcttcttc gccgtcatcg
     3961 cgtccgtctt cctcgcggtg gcgggacttc tcgccttcag ttgaatccga tggagccctg
     4021 atccatgccg aacatcctga tgcccgcact gtcgccgacg atggaagagg gcaagatcgc
     4081 caagtggctc aagaacgtgg gtgacatggt gaagtcgggc gacgtcatcg ccgaaatcga
     4141 gaccgacaag gccaccatgg aggtcgaggc cgtcgacgaa ggtccgctgt ccgccatcct
     4201 ggttcccgcc gggacggaaa acgtgaaggt caacacgccg atcgccgtga tcggcgtggc
     4261 ggatgctgcg ccggcgccga aacctgcagc tgcggcaaca cccgcggccg ccgcgccggc
     4321 ggctcccgca ccgacctttg cgggtcccga catcgcggcg acgcccgacg agaatcccga
     4381 ggtaccggcc ggcaccgaga tggtcatgat gacggtacgc gaggcgatcc gcgaggcgat
     4441 gacggaagaa atgcgccgcg acaaggacgt gttcctgatg ggcgaggaag tgggccagta
     4501 ccagggcgcc tacaaaatct cgcaaggcat gctcgacgag tttggacctc aacgagtgat
     4561 cgacacgccg atcaccgagc acggcttcac cggaatcgcg gttggtgccg ccttcgccgg
     4621 actgaaaccc atcgtcgagt tcatgacctt caacttctcc atgcaggcca tcgaccacat
     4681 catcaattcc gcggccaaga cgctgtacat gtcgggcggc cagatgggat gccccattgt
     4741 attccgtggc ccgaatggtg ccgccgcgcg cgttgccgcc cagcacagcc aggactttgc
     4801 ggcctggtac ggccatatcc cgggactgaa ggttgttgcg ccttatacgg cagcggacgc
     4861 caagggcctg atgaaggcgg cgatccgcga tcccaatccg gtggttttcc tcgaaaacga
     4921 aattctctat ggcaagagct tcgaggttcc gaagcttgaa gattgggtgg tgcccatcgg
     4981 caaggcgcgc atcgccaggg ccggccggca tgtgaccatc gtgtcgttcg gaatcggcat
     5041 ggcctatgcg ctggccgcgg ccgagaagct ggctgccgat ggcatcgagg ccgaggtcat
     5101 cgacctgcgc accatccgcc cgatggatat cgccacggtt gtcgcgagcg tgaaaaagac
     5161 aaaccgctgc gtggcggtgg aggagggctt cccgcagttc tcggtgacca gcgagattgc
     5221 cgcccagatc atgacac
//
LOCUS       DBNGVR010000096         3995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1410186, whole genome shotgun sequence.
ACCESSION   DBNGVR010000096 DBNGVR010000000
VERSION     DBNGVR010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1410186"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..274)
                     /locus_tag="AC16A0_02145"
     CDS             complement(89..274)
                     /locus_tag="AC16A0_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HIR3104160.1"
                     /translation="MDPDEPTKKKANMVIGENLEAISLAELEQRIHALESEIQRVRAE
                     IGRKLASRNAADAFFRS"
     gene            361..1341
                     /locus_tag="AC16A0_02150"
     CDS             361..1341
                     /locus_tag="AC16A0_02150"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR3104161.1"
                     /translation="MQQMTAIAITRPGGPEVLQPVKIAVPVPGPGQVLVHVKAAGINR
                     PDVLQRKGAYPPPPGAPDTPGLEIAGEVAGLGPGVKRYEVGDRVCALVPGGGYAEFAV
                     VHEDNALPVPPGLSFAEAAAIPETFFTVWTNVFDRGRLAAGETLLIHGGSSGIGTTAI
                     MLAKALGARVVVTAGSDAKCKACLDLGADLAINYRTRDFVAELAGQSIQPDVILDMVG
                     GDYVARNLKAAALHGRIVNIAFQAGSRVEVDLMPIMLKRLTLTGSTLRPRSVAEKAEI
                     ARALEETVWPLIAAGQVSPRIYRTFPLAEAAAAHALMESSEHIGKIVLLA"
     gene            1458..2183
                     /locus_tag="AC16A0_02155"
     CDS             1458..2183
                     /locus_tag="AC16A0_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HIR3104162.1"
                     /translation="MSRAPLMPKATAVWLVENTALSFRQIADFCSLHELEVKGIADGD
                     VAAGIKGMDPVTGGQLTRDEIARGEADASYRLKILESKVNIPVQRSTAGPRYTPVSRR
                     GDRPDAIAWLLRYHPELPDSAIMKLVGTTKSTIASVKDRSHWNSSNIKPVDPVSLGLC
                     SQLDLDFAVQKAARKAGKVVPDAAAPRTLIPAAEIEGTAAYAAAEAAREPEPQAEPER
                     EKTYDADSVFAKLKSLKRPEDGE"
     gene            2185..2784
                     /locus_tag="AC16A0_02160"
     CDS             2185..2784
                     /locus_tag="AC16A0_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HIR3104163.1"
                     /translation="MRILIVGATGTVGKAAAAELGRRHEIIQAGRTSGDVTVDLMDEA
                     SIRAMFKTAGKLDAVVATAGHTHFGPLAKMTAADFRKGLDDKLMGQINLTLIGQHLLN
                     DNGSITLTSGVLDRDPIRQGANAAAVNGAIGAFVRSAAIELPRGLRINVVSPGLLEES
                     VKKYDGFFPGHEPVSSARVGLAYAKSVEGAITGQVIIVA"
     gene            2839..>3995
                     /locus_tag="AC16A0_02165"
     CDS             2839..>3995
                     /locus_tag="AC16A0_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3104164.1"
                     /translation="MRDSRFDVLFEPVKIGPVTAPNRFYQVPHCTGMGYGLPATLAAM
                     RGIKAEGGWGVVNTEYCSIHPTSDDMPAPFASLWDEGDARNMAVMAEAVHAHGALAGV
                     ELWHGGLRSSNLQSRETSLGPQSLPTASDPWQCARMDLSDIRMLRQWHRAAALRARAA
                     GMDIVYVYAAHTYLLAQFLDRDINQRTDEYGGSAENRARLVRELLSETREAVGDRCAV
                     AIRIEADHEDGGGEAERAELLASLAPLVDLFNITVSDYSHEMGVSRFIKEGSLESRIA
                     HVRKVTGKPVVSVGRFTSPETMLSQVKRGIVDLVGAARPSIADPFLPAKIREGREDDI
                     RECIGCNICYACDGRGVAIRCTQNPTMGEEWRRGWHPEVIAPRASEDSILIV"
BASE COUNT          708 a         1283 c         1287 g          717 t
ORIGIN      
        1 agaaagtctg gattgcaatt attcatatta aggttaattc cttaccaaaa ggttaacact
       61 tcccttcggg taagtagaat tcctcggctc agctccggaa aaatgcgtca gcagcattgc
      121 gagaggccag tttccgccca atttcggctc tgactctctg aatctccgac tccaaggcgt
      181 gaatccgctg ttccagttcg gcaagcgaaa tggcttcgag gttttccccg ataaccatgt
      241 tggccttctt cttggttggt tcatccgggt ccatgggcat gctcctttgc acctgacttt
      301 gtcattgcag ggcagtaacg atcaacccat tctgtgagcc aagtcgcaag gagtgccttc
      361 atgcagcaga tgactgccat tgccatcacg aggccgggtg ggcctgaagt cctgcagccg
      421 gtcaagattg cggttcccgt tcccggaccg ggacaggtcc tggtacatgt gaaggccgca
      481 gggatcaatc ggccggacgt cctccagcgc aagggggcct atccgccgcc gccgggagct
      541 ccggacacgc cgggccttga gattgccggc gaagtggcag gtcttggacc tggcgtgaag
      601 cgctatgagg taggcgaccg cgtctgcgcc cttgtgccgg gcgggggcta tgccgagttc
      661 gcggtggtgc atgaggacaa tgccctgccc gtgccgccag gcctttcctt cgccgaggcc
      721 gccgcgatcc ccgagacctt cttcaccgtg tggaccaatg tcttcgaccg gggccggctg
      781 gcggctggcg agaccctgct gatccatggc ggctccagcg ggatcggtac gacggccatc
      841 atgctggcca aggcgctggg cgcccgcgtg gtggtgacgg cggggtccga cgcgaagtgc
      901 aaggcttgcc ttgatctcgg cgccgatctg gcgatcaact accggacccg ggatttcgtc
      961 gccgaactgg ccgggcaatc cattcagccc gatgtcatcc tcgacatggt cggcggcgat
     1021 tacgtcgccc gcaacctcaa ggccgcggcc ctgcatgggc ggatcgtgaa cattgccttc
     1081 caggccggtt cgcgggtcga ggtggacctg atgccgatca tgctgaagcg cctgacgctc
     1141 acgggctcca cgctccggcc gcgctccgtg gcggagaagg ccgaaattgc ccgcgcgctt
     1201 gaggaaacgg tctggccgct gatcgcggca ggacaggtca gcccgcggat ctaccggacc
     1261 tttccgctgg ccgaggccgc cgctgcccat gcgctgatgg aatcctcgga acacatcggc
     1321 aagatcgtgc tgctcgcctg accggccggg cgaaaagaca tttgcgtcgg cgcgcctgct
     1381 gaactatatg gggccatccc caacaagttg tccttgagac agggaacctc agccggttcc
     1441 caaggagcga aaaaaccatg tcccgcgcac ccctcatgcc caaggccacc gccgtctggc
     1501 tggtggagaa caccgcgctc agctttcgcc agatcgccga tttctgcagc ctgcatgagc
     1561 tggaggtgaa gggcatcgcc gatggcgacg tggccgccgg catcaagggc atggacccgg
     1621 tcaccggcgg gcagctcacc cgcgacgaga tcgccagggg ggaagccgac gcgagctacc
     1681 ggctgaagat cctcgaatcc aaggtcaata tccctgttca gcgctcgacc gcaggtccgc
     1741 gctatacgcc cgtgtcgcgc cgcggcgacc ggccggacgc catcgcctgg ctgctgcgct
     1801 accatccgga gcttcccgat tctgccatca tgaagctggt cggcacgacc aagtcgacca
     1861 tcgcctcggt caaggaccgc agccactgga actcgagcaa catcaagccg gtcgatccgg
     1921 tgtcgctggg cctgtgctcg cagctcgacc ttgatttcgc ggtccagaag gccgcccgca
     1981 aggcgggcaa ggtggtgccg gacgcggccg cccccaggac ccttatcccc gccgcggaga
     2041 tcgaaggaac agccgcctat gccgccgccg aagccgcgcg cgagcccgag ccgcaggccg
     2101 aacccgagcg cgagaagacc tatgatgccg actcggtctt cgccaagctg aagagcctga
     2161 agcgtcccga ggacggtgag taagatgcgg atcctcatcg tcggcgcgac aggtacggtc
     2221 ggcaaggctg cagcggccga gcttggcagg cgccacgaga tcatccaggc cgggcgaacc
     2281 tccggcgatg tcacggttga cctgatggac gaggcctcga tccgcgcgat gttcaagacg
     2341 gcgggcaagc tcgatgccgt ggttgcaacg gccgggcata cccatttcgg tcccctggcc
     2401 aagatgaccg ctgccgattt ccgcaagggc ctcgacgaca agctgatggg ccagatcaat
     2461 ctcacgctga tcggccagca tcttctgaat gacaatggct cgatcaccct taccagcggc
     2521 gtgctggacc gcgacccgat ccgtcagggc gccaacgccg cggcggtcaa tggtgcgatt
     2581 ggcgcattcg tcaggtcggc ggccatcgag ctcccgcgcg gcctccgcat caacgtggtg
     2641 agcccgggcc tgctcgaaga atccgtgaag aagtacgacg gattcttccc tggccatgag
     2701 ccggtgtcgt cggcgcgggt cggtctcgcc tatgccaaga gcgtcgaggg cgcgatcacc
     2761 gggcaggtca tcatcgtcgc ctgatttgcg tctgccgctt ctcggctgct agccttgggc
     2821 agatggagaa ggtgcctgat gcgtgattcc cgttttgacg tgcttttcga gccggtgaag
     2881 atcggcccgg ttactgcacc caaccgcttc tatcaggttc cgcactgcac cggcatgggc
     2941 tatggcctgc ctgccacgct ggctgcgatg cgcggcatca aggccgaagg cggctggggt
     3001 gtggtgaaca ccgagtattg ctccatccat cccacatcgg acgacatgcc ggcgcccttt
     3061 gccagcctgt gggacgaggg cgatgcgcgc aacatggcgg tgatggcgga ggcggtgcac
     3121 gcgcatggcg ccctggccgg cgtcgaactc tggcacggcg ggttgcgttc atccaatctg
     3181 cagtcgcgcg aaacgtctct cggtccgcag agcctgccga cggcctccga tccctggcaa
     3241 tgcgcccgca tggatctctc cgacatccgc atgctgcggc aatggcatcg ggctgctgcc
     3301 ctgcgcgccc gggcggccgg catggacatc gtctatgtct acgctgcgca cacctatctc
     3361 ctggcgcagt tcctcgatcg cgacatcaat cagcggacgg atgaatacgg cgggtcggcg
     3421 gaaaaccgcg cgcggttggt gcgcgagctg ctgagcgaga cgcgcgaggc ggttggcgac
     3481 cgctgtgccg tggcgatccg catcgaagcc gatcatgaag atggcggcgg cgaagcggaa
     3541 cgcgccgagc ttctggcaag ccttgcgccc ctggtggatc tcttcaacat cacggtttcc
     3601 gactacagcc acgaaatggg cgtctcgcgc ttcatcaagg agggttcgct cgaaagccgc
     3661 atcgcgcatg tccgcaaggt gacgggaaag cccgtggtca gcgtcggccg attcacctca
     3721 cctgaaacca tgttgagtca ggtgaagcga ggcatcgtgg atctcgtcgg ggcggcgcgt
     3781 ccgtcgatcg ccgatccttt cctgccggca aagatccgcg aaggccgtga ggacgacatc
     3841 cgcgaatgca tcggatgcaa catctgctat gcctgcgacg gacgcggcgt ggccatccgc
     3901 tgcacccaga acccgaccat gggcgaggaa tggcggcgcg gctggcatcc cgaggtcatc
     3961 gcgccgcggg cgtcggagga ttcgattctc atcgt
//
LOCUS       DBNGVR010000097         4267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4946155, whole genome shotgun sequence.
ACCESSION   DBNGVR010000097 DBNGVR010000000
VERSION     DBNGVR010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4946155"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..350)
                     /locus_tag="AC16A0_02170"
     tRNA            complement(275..350)
                     /locus_tag="AC16A0_02170"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Phe,seq:gaa)
     gene            complement(400..1014)
                     /locus_tag="AC16A0_02175"
     CDS             complement(400..1014)
                     /locus_tag="AC16A0_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104165.1"
                     /translation="MGMLASLKRFFAPQPIADLAALQQFLESRAAYLVQKSITEYSQA
                     RAGVLFSTLMREPIYIEGYERARWLSYPAAISMVTEMAEGSLRERSGSAPGALDRGLL
                     KLVGAILAQYPGPSRLGVDFWREAADRVTRDLAQAALGPPRAVQNIPMIRSKEIYDVL
                     PVNDHLKRHDFQMFRNTIRFHLTELKVEFEERSEPARLAAILSA"
     gene            1204..1461
                     /locus_tag="AC16A0_02180"
     CDS             1204..1461
                     /locus_tag="AC16A0_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HIR3104166.1"
                     /translation="MAESKAEGHWAATSRLMWITLAIWAFFSFVIHFFVNSLNEIVIF
                     GFPLGFYFAAQGSLIVFVLLLFWFARKQDDIDQEFGFAEEE"
     gene            1464..3659
                     /locus_tag="AC16A0_02185"
     CDS             1464..3659
                     /locus_tag="AC16A0_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3104167.1"
                     /translation="MAQAQGETGFLANLGKVYAIYTGGFLAFVILIAILEQLGVPNRV
                     LGYMFVGFTILVYALIGILSRTAQVSEYYVAGRKVPAVYNGMATGADWMSGASFVGMA
                     GTLYALGYDGLAFVLGWTGGYVLVSVLLAPYLRKFGAYTVPDFLGARYGGNIARFLGV
                     LVLLACSFTYVVAQIYATGIIASRFLGMSFEVAVYAGLAGILVCSMLGGMRAVTWTQV
                     AQYIVLIVAYLVPVVILSTQKYGVPLPQIMYGQALADIAARETQMVTEGLAKLASDGG
                     TLKPHISAFLTYDRLNFFGLIFCLMVGTASLPHILMRYFTTPSVRDARKSVAWSLFFI
                     FLLYFTAPAYAAFAKLEVYNNVIGQSLDALPAWISTYSQLGLVDIYNVSLSKLGAAIA
                     ALKDAGAADAASVAAALSGAGKTAALASWSALSAPVQAVVFEAFQANNAVTAAELFKP
                     AIAAASQAAGNTTGLLTHGGLAINNDVIVLSTPEIAGMPYVIAGLVAAGGLAAALSTA
                     DGLLLAIANALSHDIYYKMIDTQAPTARRLIVSRILLVVVAVIAAYVASTKPADILAM
                     VSWAFSIAAAGLFPALVLVVWWKRANRTAAIVGIIAGFGVCLYYLVATRYYAVSFYEM
                     WSGLSGANEAAVAKYAELKAAFEGAAPEAKAAAWKAFDLHAQKVANWFGVSNISSALF
                     GLPAGFIGMILGSLLTAAPSKAMQEFIDEIRRPKGKELMEGGNLVKEIR"
     gene            3734..4036
                     /locus_tag="AC16A0_02190"
     CDS             3734..4036
                     /locus_tag="AC16A0_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104168.1"
                     /translation="MNPLFQYWYFHLPNFVLAAVMYTLLGRLLLSLFVPPDWKNYIWR
                     AFVRITEPAVRATRFVTPQALPQVIVLVFAILWVMLLRIALFAVLGSLGLLPSVNG"
     gene            4041..>4267
                     /locus_tag="AC16A0_02195"
     CDS             4041..>4267
                     /locus_tag="AC16A0_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104169.1"
                     /translation="MSQNHIVVIVGLAILNGIFSPALIAVFALNGLWYPFFLPPMLPL
                     IFAFSSLILSTMTLMVAGVPAALYERLAGGG"
BASE COUNT          708 a         1400 c         1266 g          893 t
ORIGIN      
        1 tggtgcccag gggcggaatc gaaccaccga cacgcggatt ttcatccccg actttgtcgg
       61 tctcggtttg aatgaggagt cgtctgctac cgctcggctt tgccatcaac gtctcaggtc
      121 tgatgaacgc caaaggccgg caactcaagc tcggcactct tggcgacctt catgcaaccg
      181 ctcgaaaaac ccagaatgac acccagcgcg aaaatcggca attcgcggcg aagcacgact
      241 tcgcagcaac tctgtgattt tgcagaagaa atagtggtgc ccaggggcgg aatcgaacca
      301 ccgacacgcg gattttcaat ccgctgctct accaactgag ctacctgggc acgcgttgac
      361 ggggcggtgt ataggggagg cctgcgccat tggcaagcct caagcggaca ggattgccgc
      421 cagcctggcg ggctccgacc gctcctcgaa ctcgactttc aattctgtca gatggaagcg
      481 gatggtgttg cgaaacatct ggaaatcatg gcgtttcaga tggtcattta ccggcaggac
      541 atcgtagatt tccttgctgc ggatcatcgg gatgttctgg accgcacgcg gcgggccgag
      601 ggcggcctgg gcgaggtcgc gcgtcacccg gtcggctgct tcgcgccaga aatccacgcc
      661 gagcctggag ggacccgggt actgtgcgag gatggcaccg accagcttca gcagcccacg
      721 gtcgagcgca cctggcgcac tgccgctgcg ttcccgcagg ctgccttccg ccatctccgt
      781 caccatcgag atggccgccg ggtaagagag ccagcgcgcc cgctcatagc cctcgatgta
      841 gatcggctcg cgcatcaacg tcgagaacag aacgccggcg cgggcctgcg agtactcggt
      901 gatggacttc tgaaccaggt aagctgcgcg actttccaga aattgctgca gcgccgcaag
      961 atcggcgatt ggttgcggcg caaaaaaccg cttcaaggat gccagcatgc ccatgagact
     1021 tcctcgcact ccacgtcggg cagggcaagc cccacgacag aagccctccg aaagtcgaat
     1081 caggggtggt gcagagctgt gttagaaacg cctaacgtcc gtgcactccg ctgcggcagc
     1141 atgcagggac gtccgccatc ggcccagcag gggccatgca acatctttgg gagggaaaga
     1201 cctatggcag aatcgaaagc cgagggtcac tgggcagcaa cgtcgaggct gatgtggatc
     1261 acattggcga tctgggcgtt cttctccttc gtgatccact tctttgtaaa ctcgctgaac
     1321 gagatcgtga tcttcggatt cccgcttggc ttctatttcg cggcccaggg ttcgctcatc
     1381 gtcttcgtcc tgctgctctt ctggttcgca cgcaagcagg atgacatcga tcaggaattc
     1441 ggcttcgcgg aggaggagtg accatggcac aagctcaagg tgaaaccggc ttcctcgcaa
     1501 atctcggcaa ggtctacgcc atctatacgg gcggcttcct cgccttcgtg atcctcattg
     1561 ccatcctcga acagctgggc gtacccaacc gcgtactcgg ctacatgttc gtcggcttca
     1621 cgatcctggt ctacgccctg atcggcatcc tgtcgcgcac cgcgcaagtg tccgagtact
     1681 acgtcgccgg ccgcaaggtc cctgcggttt acaacggcat ggcgaccggc gccgactgga
     1741 tgtcgggcgc ctccttcgtc ggcatggccg gcacgctcta tgcgctgggc tacgatggcc
     1801 tggccttcgt gctgggctgg accggcggct atgtgctcgt ctctgttctg ctcgcgccct
     1861 atcttcgcaa gttcggtgcc tatacggtgc ccgacttcct cggcgcgcgc tacggcggca
     1921 acatcgcccg cttcctcggc gttctcgtgc tgctcgcctg ctccttcacc tatgtggtgg
     1981 cgcagattta cgcgaccggc atcatcgcct cgcgcttcct tggcatgtcc ttcgaggtcg
     2041 cggtctatgc gggtctcgcc ggcatcctgg tctgctcgat gctgggcggc atgcgtgccg
     2101 tgacctggac ccaggtggcg caatacatcg ttctgatcgt ggcctatctc gtcccggtcg
     2161 tcatcctgtc gacccagaag tatggcgttc cgctgccgca gatcatgtat ggccaggcac
     2221 tggccgacat cgcggcgcgc gaaacccaga tggtcaccga agggctggcc aagctggcat
     2281 cggatggcgg cacgctgaag ccgcatatct cggccttcct gacctatgac aggctgaact
     2341 tcttcggcct gatcttctgc ctcatggtgg gtacggcctc cctgccccac atcctcatgc
     2401 gctacttcac caccccctcg gtgcgtgacg cgcgcaagtc ggtggcctgg tcgctgttct
     2461 tcatcttcct gctctacttc acggcgcccg cctatgcggc cttcgcgaag ctggaggtct
     2521 acaacaacgt gatcgggcag agtcttgacg cgctgccggc ctggatcagc acctattcgc
     2581 agctgggcct ggtcgacatc tacaacgtgt cgctgtccaa gcttggcgca gccatcgcgg
     2641 cgctgaagga tgcaggcgct gccgatgcgg cgagcgtcgc ggccgccctg tcgggcgccg
     2701 gaaagacagc ggcactggcc agctggtcag ccttgtccgc accggtccag gccgtggtgt
     2761 tcgaggcctt ccaggccaac aatgccgtga ccgccgccga gttgttcaag cccgccattg
     2821 ccgccgcctc gcaggctgcc ggcaatacca cgggcctgct cacccacggc ggcctcgcga
     2881 tcaacaacga cgtgatcgtg ctctccactc cggagatcgc gggcatgccc tatgtgatcg
     2941 cgggcctggt agcggccggc ggccttgccg cagcgctctc gactgcggac ggcctgttgc
     3001 tggctatcgc caatgctctg tcgcacgaca tctactacaa aatgatcgat acccaggcgc
     3061 cgactgctcg ccgcctgatc gtatcgcgca tcctgctggt ggtcgtggcg gtgatcgcgg
     3121 cctatgtggc ctcgaccaag ccggccgaca ttctggccat ggtctcctgg gccttctcca
     3181 tcgctgcagc gggcctcttc ccggccctag tgctcgtcgt gtggtggaag cgcgccaacc
     3241 ggacagccgc catcgtcggc atcatcgcgg gcttcggcgt ctgcctctac tacctcgtgg
     3301 ccacccgcta ctatgcggtc agcttctacg agatgtggag cggcctgtcg ggtgccaacg
     3361 aggctgctgt cgccaagtac gccgaactca aggcggcctt cgaaggcgcg gcaccggaag
     3421 ccaaggctgc ggcctggaag gctttcgatc tccatgccca gaaggtggcc aactggttcg
     3481 gcgtgtcgaa tatctcctcg gcgttgttcg gccttccggc cggcttcatc ggcatgatcc
     3541 tgggcagcct gctgaccgcg gcgccatcca aggccatgca ggagttcatc gacgagatcc
     3601 gccgcccgaa gggcaaggaa ctcatggaag gcggaaacct ggtcaaggaa atccgctgat
     3661 ctcccgcaca accgaaacgg ggcccttgcg ggccccgttt tgcttttgga catccctcgg
     3721 ctaggctcgt atcatgaacc cgctcttcca gtactggtat tttcacctgc cgaatttcgt
     3781 gctggcggcg gtcatgtaca cgctgctggg acggctgctg ctgtcgcttt tcgtgccacc
     3841 cgactggaag aactacatct ggcgcgcctt cgtccgcatc accgaaccgg ccgtgcgggc
     3901 aactcgcttc gtcacgcccc aggctctgcc gcaggtgatc gtcctggtct ttgccattct
     3961 ctgggtcatg ctgctgcgca ttgccctctt cgcggtcctc ggcagccttg gcctcctgcc
     4021 ctccgtcaac gggtgatccc atgtcgcaga accatatcgt cgtaatcgtc ggcctggcca
     4081 tcctgaacgg gatcttctcg cctgcgctga tcgcggtctt cgccctgaat ggtctctggt
     4141 atcccttttt ccttccaccg atgctgccgt tgatctttgc cttttcctcg ctgatcctgt
     4201 cgaccatgac cttgatggtc gccggcgtgc cggccgccct ctacgagcga cttgccggcg
     4261 gcggaag
//
LOCUS       DBNGVR010000098         4499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5142866, whole genome shotgun sequence.
ACCESSION   DBNGVR010000098 DBNGVR010000000
VERSION     DBNGVR010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5142866"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="modA"
                     /locus_tag="AC16A0_02200"
     CDS             complement(<1..668)
                     /gene="modA"
                     /locus_tag="AC16A0_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3104170.1"
                     /translation="MLTRRMFGLAALGLAALISTAPVPALAAEKVTVFAAASLKNALD
                     EAAAAWTAETGKTATISYAASSALAKQIEEGAPADIFFSADLDWMKYLTDRKLVAEGS
                     EIQLLGNEIVLIAPADSKAETVIAKDFPLTTLLGDGKLAMGDVKAVPAGKYGKAALES
                     LGVWSSVEGKVAQAENVRAALKLVATGEAPLGIVYQTDARAEPKVKVVGTFPSDSHPP
                     IIYPV"
     gene            797..2005
                     /locus_tag="AC16A0_02205"
     CDS             797..2005
                     /locus_tag="AC16A0_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3104171.1"
                     /translation="MSASTPPKRNSRQGLSTRAIHLGYDPATAQGALTPPIYQTSTYA
                     FETVEQGAEYFAGLKPGYVYGRTRNPTQALLEQRVASLEGGEAGLALASGMAAISSCF
                     WTLLQAGDRIICDKILYGNTFTLASQGLPRFNVTADFVDLTDLDALTAAAARPFKAIY
                     FETPANPNLRLIDIAAVSAVAAKAGALTIVDNTFATPVLQRPLEHGADLVVHSATKYL
                     GGHGDLLAGVVVGGEDMVKQIRMHGLRYMTGSTISPLTAFLVLRGLKTLELRVNQHAK
                     SAAAIADFLAAQAAVSKISYPAHASHSQHGVYRKQMSAGGGLISFEIDGGMERGRTFM
                     NALKLISRAVSLGDAETLIQHPASMTHASYGPEERARHGISDGLIRMSVGLENVEDIL
                     DDLDQALRQI"
     gene            complement(2006..3289)
                     /locus_tag="AC16A0_02210"
     CDS             complement(2006..3289)
                     /locus_tag="AC16A0_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HIR3104172.1"
                     /translation="MPIDADLRQRIASSAAIGFPDQIAFTQQLVRFPSTRGNEHTVQD
                     FMARELRARGMAVDVFAMEDAALARHPGGSRISDTHSRAPIVVGIHRPRTETGRSLIL
                     QGHVDVVPPGPLDIWTTPPFDPVIRDGWMSGRGAGDMKAGVASAVFALDALRRIGLQP
                     AATVYMQSVVEEESTGNGALMTHLRGYKADAALVPEPGNEGLTRANLGVLWFQLEVRG
                     IPVHVANMGTGANAIDAAYRVIGALRQLEERWNAKAKAHDLFGAHHHPINLNLGKIEG
                     GDWASSVPAWCRIDCRIAFLPGTSAAAAAAEIEACITEFARRDSFLANNPPRVAWNGF
                     FAEGYRLEPGSGAERTLAAAHQAVTGQPLEDRLVTAYLDARVYALYDRIPVLNYGCIA
                     ENYHGFDERVDLASVLRTTQVIAQFVADWCGVEPA"
     gene            3400..3816
                     /locus_tag="AC16A0_02215"
     CDS             3400..3816
                     /locus_tag="AC16A0_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HIR3104173.1"
                     /translation="MSPVSFILNIFWIACGGLWMAAGWLLAAVIMAITIVGIPWAKAA
                     VKMAEYTLLPFGFKAVTRPTEDIGTGPLGFVGNAIWLIFAGWWLALAHVITAVLLAIT
                     LIGIPFAWAHLKLAGIALWPIGKTIVPIDTPEPSTV"
     gene            3924..>4499
                     /locus_tag="AC16A0_02220"
     CDS             3924..>4499
                     /locus_tag="AC16A0_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-keto acid cleavage family enzyme"
                     /protein_id="HIR3104174.1"
                     /translation="MRKKSKAIITCAITGSIHTPSMSPYLPVTPDEIAAQAIEAAEAG
                     AAILHLHARDPETGKPSGAPDTFNRFLPRIKQSCNAIVNITTGGGQGMSVEERIMAAE
                     AFSPEMTSLNMGTMNFGLFPMLNRYNEFKHPWERAHLEGSKDFIFRNTFGDIEKILLK
                     LGDGHGVRFEFECYDIGHLYTLAHFLDRKLVK"
BASE COUNT          774 a         1479 c         1423 g          823 t
ORIGIN      
        1 accggataaa tgatcggcgg gtggctgtca gatgggaagg ttccgacgac cttcaccttg
       61 ggttcggctc tcgcatccgt ctggtagacg atgccaagcg gggcctcgcc ggtggcgacc
      121 agcttcagcg cggcgcggac gttctccgct tgggcaacct tgccctcgac ggaggaccag
      181 acgcccagcg actccagagc cgccttgccg tacttgcctg ccggcacggc cttgacatcg
      241 cccatggcga gcttgccgtc gccgagcagg gtggtcaggg gaaagtcctt ggcgatgact
      301 gtctcggcct tggaatcggc gggcgcgatc agcacgatct cattgcccaa caactgaatc
      361 tcgctgccct cagccaccag cttgcggtcc gtcagatatt tcatccagtc caagtcggcg
      421 gagaagaaaa tgtcggcggg cgcgccttcc tcgatctgtt tggccagggc ggagctggcg
      481 gcataggaga tcgttgctgt cttgccggtt tcggccgtcc aggcggcggc ggcctcgtcg
      541 agcgcatttt tcaggctggc ggccgcgaag accgtgacct tctcggcggc cagcgccggc
      601 actggggcgg tggagatcag ggcggcaagg ccgagggcgg cgaggccaaa cattctgcgc
      661 gtgagcatca attcgtccat tcgttatatc catagggata taacactagc ttcaactgtg
      721 caacacccat gcactcgtgt ttccatttgc cccgcccagc cgctaagggg ggagcagcac
      781 gcgaggacct ccatcaatgt cagccagcac accgccgaaa cgcaactccc ggcaagggct
      841 ctcgacccgg gccattcacc ttggctacga ccccgccacc gcccagggcg cgctgacgcc
      901 gccgatctac cagacctcga cctatgcttt cgaaacggtc gagcaggggg ccgagtactt
      961 tgcaggcctc aagcccggct atgtctatgg ccgcacccgc aatcccaccc aggcgctgct
     1021 ggagcaacgg gtggcaagtc tcgaaggcgg cgaggcgggg ctggcgctgg cctccggcat
     1081 ggcggcgatc agctcctgct tctggaccct gctgcaagcg ggcgaccgga tcatctgcga
     1141 caagatcctc tacggcaata cctttacgtt ggcctcgcag ggcctgccgc gcttcaacgt
     1201 gacggcggac ttcgtcgacc tgacggatct cgatgcgctg acggccgccg cggccaggcc
     1261 cttcaaggcg atctatttcg agacgcccgc caatcccaat ctccgcctca tcgacattgc
     1321 cgccgtcagc gccgtcgccg ccaaggcggg cgcgctgacg atcgtcgaca atactttcgc
     1381 cacgccggtt ctgcagcggc cgctggaaca cggcgccgat ctcgtggtcc attcggccac
     1441 caaatatctc ggcggccatg gcgatcttct cgccggcgtg gtggtgggcg gtgaggacat
     1501 ggtgaagcag atccgcatgc atggcctgcg ctacatgacc ggcagcacca tctcgccgct
     1561 caccgccttc cttgtgctgc gcgggttgaa gacgctcgag ctgcgcgtca accagcatgc
     1621 gaagtcggcg gcggcaatcg cggattttct tgccgcgcag gcggctgtct cgaagatctc
     1681 ctatcccgcc catgcctcgc attcgcagca cggcgtctac cgcaagcaga tgagtgccgg
     1741 cggtggtctc atcagcttcg agatcgacgg cggcatggaa cgtggccgca ccttcatgaa
     1801 tgcgctgaag ctgatcagcc gcgcggtcag cctcggcgat gccgagacgc tgatacagca
     1861 tcccgccagc atgacccatg cgtcctatgg tccggaagaa cgggcgcggc acggaatctc
     1921 cgacggtctc atccgcatgt cggtcggtct cgagaatgtc gaggacatcc tcgacgatct
     1981 cgaccaggcc ttgcgccaga tctgatcagg ccggttccac gccacaccag tcggcgacga
     2041 attgcgcgat gacctgcgtg gtgcgcagca ccgaggccag gtccacgcgc tcgtcgaagc
     2101 catggtaatt ctcggcgatg cagccgtagt tcagcacggg gatcctgtcg tagagcgcat
     2161 agacccgtgc atcgagatag gcggtgacga ggcgatcctc cagcggttga ccggtcaccg
     2221 cctgatgcgc cgccgcgagc gtcctttcgg ctcctgatcc cggctccagc ctgtagcctt
     2281 ccgcgaagaa gccgttccag gccacgcgcg gcgggttgtt ggcgaggaag gagtcacggc
     2341 gtgcgaattc ggtgatgcag gcctcgattt cggctgcggc cgcggctgcc gatgttccgg
     2401 gcaggaaggc gatgcggcag tcgatgcggc accaggccgg gaccgacgag gcccagtcgc
     2461 caccctcgat cttgccgaga ttgagattga tcgggtggtg gtgcgcgccg aagagatcat
     2521 gcgctttcgc cttggcgttc cagcgctcct cgagttggcg aagggcgccg atcacccggt
     2581 atgccgcgtc gatggcattg gcaccggtgc ccatgttggc cacatgcacc ggaatgccgc
     2641 gcacttcgag ctggaaccac agcacgccga ggttggcgcg tgtgagcccc tcgttgcccg
     2701 gctcgggaac gagggccgca tccgccttgt agccgcgcag gtgggtcatc agcgcgccgt
     2761 tgcctgtcga ttcctcctcc accaccgact gcatgtagac cgtggcggct ggctgcaggc
     2821 cgatgcggcg caaggcatcc agcgcgaaga cggcagaggc cacgccggcc ttcatgtcgc
     2881 cagcaccacg tcccgacatc cagccgtcgc ggatcaccgg atcgaagggc ggagtggtcc
     2941 atatgtcgag cggccccggc ggcacaacgt cgacatggcc ctgcaggatc agcgaacggc
     3001 ccgtttccgt ccgcgggcgg tggatgccga ccacgatggg cgcccgcgaa tgcgtgtccg
     3061 agattctcga tccgcccgga tgccgggcga gggcggcgtc ctccatggcg aagacgtcga
     3121 ccgccatgcc gcgcgcgcga agttcgcgcg ccatgaaatc ttgtaccgtg tgctcgttgc
     3181 cgcgggtcga gggaaagcgc accagctgct gggtgaaggc gatctggtcg gggaagccaa
     3241 tggcggcgga ggaagctatg cgctggcgga ggtcggcgtc gatgggcatg atgtctccca
     3301 cgggtttttc ccgatcttga ccgctggcgg ctcatttcgc aattgctgga atcgtcggga
     3361 tggcctcact atatgagcgc tgcaaaggag accgatgacg tgagccccgt ttcattcata
     3421 ttgaacatct tctggattgc ctgcggcggc ctgtggatgg ctgcgggctg gcttctggcg
     3481 gccgtcatca tggccatcac catcgtcggc attccctggg ccaaggctgc ggtgaagatg
     3541 gccgagtaca cgctgcttcc cttcggcttc aaggccgtta cccgtcccac cgaagacatc
     3601 ggcacggggc cgctgggttt tgtcggcaac gccatctggc tgattttcgc cggctggtgg
     3661 ctggcccttg cgcatgtgat cacggccgtg cttctggcga tcaccctgat cggcattccc
     3721 ttcgcctggg cgcatctcaa gctggccggt attgcgctct ggcccatcgg caagaccatc
     3781 gttcccatcg acacgccgga gccttccacc gtctgacccg ccgtgcggtc tggcgcgggg
     3841 catgaaggcc ctgcaaaact acttggtgac tgtggcttcg gcgggcggct attgtcccgc
     3901 cgaagcagcc agacaggttc accatgcgga agaaatccaa ggccatcatc acctgcgcca
     3961 tcaccggctc gatccatacg ccgtcgatgt cgccctatct cccggtgacg ccggacgaga
     4021 tcgccgccca ggctatcgag gccgccgagg cgggcgctgc catcctccat ctccatgcgc
     4081 gcgatccgga gaccggcaag ccctccggcg cgcccgacac gttcaaccgc ttcctgccgc
     4141 gcatcaagca gtcctgcaac gccatcgtga acatcaccac ggggggcggg cagggcatgt
     4201 cggtggaaga gcgcatcatg gcggccgagg ccttctcgcc tgaaatgacc tcgctcaaca
     4261 tgggaaccat gaacttcggc ctctttccca tgctcaaccg ctacaacgag ttcaaacacc
     4321 cctgggagcg cgcccatctc gaaggatcga aggacttcat ctttcgcaac accttcggcg
     4381 atatcgagaa gatcctgctc aagctgggcg atgggcatgg cgtccgcttc gagttcgaat
     4441 gctatgacat cggccacctc tacacgctgg cgcatttcct cgaccgcaag ctggtgaag
//
LOCUS       DBNGVR010000099         1979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4553756, whole genome shotgun sequence.
ACCESSION   DBNGVR010000099 DBNGVR010000000
VERSION     DBNGVR010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4553756"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="AC16A0_02225"
     CDS             complement(<1..143)
                     /locus_tag="AC16A0_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3104175.1"
                     /translation="MASLVLGIETSCDETAAAVVRRESDGRGHILSNLVLSRTQDHAP
                     YGGV"
     gene            217..1131
                     /gene="hemC"
                     /locus_tag="AC16A0_02230"
     CDS             217..1131
                     /gene="hemC"
                     /locus_tag="AC16A0_02230"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3104176.1"
                     /translation="MPLLKIGTRGSPLALAQARETRARLMAAHGMAETDIEIVVITTT
                     GDRIRDRPLSEIGGKGLFTKEIEEALFDGSIHLAVHSMKDMPARLPDGLAMAAILPRE
                     DARDAFLSPVAKSIDDLAKGATVGSSSVRRAAQLKRLRPDLNVIQFRGNVETRLRKLD
                     EGVAAATFLACAGLNRLGLSDRITSAIPSEIMLPAVAQGAVGIEIRADDSKTRDMVAA
                     INHETSAIAVDCERAFLAALDGSCRTPLAGHATLKDGRISFRGEALTHDGAHCFATTR
                     DGGVSDAARMGHEAGEEVKARGGALIAY"
     gene            1131..1841
                     /locus_tag="AC16A0_02235"
     CDS             1131..1841
                     /locus_tag="AC16A0_02235"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3104177.1"
                     /translation="MRVIVTRPEEDAGPLRQTLESLGHEAILQPLMTIAPRHDVTIPN
                     LPWQAVVATSANGIRALPDASSLHHLPMLTVGPQSLKAAQDAGFTLAEAHGGDVDGLA
                     AHVIAARRASNGPILYLSGADTAGNLQGQLAAAGFDCRRVVLYDAVPARDLGAAATAL
                     RQHKADAVLLYSPRSARIWSSLAKAAGLSAEAAAVPSLCLSRNVAAALPEGCNALVAE
                     KPENAAMLRLLEELHRTR"
BASE COUNT          347 a          667 c          655 g          310 t
ORIGIN      
        1 acgccgccat agggggcgtg gtcctgcgtc cgggacagca cgaggttcga caggatatgc
       61 ccccggccat cggactcgcg ccgcaccacg gcggcggcgg tctcgtcgca agaggtttcg
      121 atgccgagga ccaggcttgc catggcaggg gtgacgtttc cggagagagc ggttaagaag
      181 gggtccgctg tagcatctca cggaacagac ttgcaaatgc cgctcttgaa gattggaacc
      241 cgcggttcgc cacttgccct ggcccaggcc cgcgaaacgc gcgcccgcct catggccgcc
      301 catggcatgg ccgaaacgga catcgagatc gtggtgatca ccaccaccgg cgaccgcatc
      361 cgcgaccggc cgctgtccga gatcggcggc aaggggctct tcacaaagga gatcgaggag
      421 gcgctgttcg acggcagcat ccacctggcc gtccactcaa tgaaggacat gccggcgcgt
      481 ttgccggacg ggcttgccat ggcggccatc ctgccgcgcg aggatgcgcg cgatgccttt
      541 ctcagcccgg tggcaaagtc gattgacgac ctggcgaagg gcgcgacggt ggggtcgtca
      601 tcggtgcggc gcgcggcgca attgaagcgg ctgcggccag acctgaatgt tatccagttc
      661 cgcggcaatg tggaaacgcg cctgcgcaag ctggacgagg gcgttgccgc tgctaccttc
      721 ctggcctgcg caggactcaa tcggctcggg ctctcggacc gcatcaccag cgccattccc
      781 tccgagatca tgctgcccgc cgtggcgcag ggcgcggtcg gcatcgaaat ccgcgccgat
      841 gacagcaaga cgcgcgatat ggtcgcggcc atcaatcacg agaccagcgc catcgcggtt
      901 gactgcgagc gcgcctttct ggccgcgctc gacggctcct gccggacgcc gctggcgggg
      961 cacgcgacat tgaaggatgg acggatctcc ttccgcggcg aagcgctgac ccatgatggc
     1021 gcgcattgct tcgccaccac gcgcgacggc ggagtttcgg acgccgcacg catgggacac
     1081 gaggccggcg aagaggtgaa ggcgcggggc ggcgcattga tcgcctattg atgcgcgtca
     1141 tcgtcaccag gccggaagaa gatgcagggc cgctccgcca gacgctcgaa tcgctcgggc
     1201 atgaagcgat cctccagccg ctgatgacca tcgcgccgcg ccatgatgtg acgatcccga
     1261 atctcccatg gcaggctgtg gtggcaacca gcgcaaatgg cattcgcgca ctgcccgatg
     1321 cgtcatccct gcatcatctg cccatgctga cggtcgggcc gcaatccctc aaggccgcgc
     1381 aggacgccgg ctttacgctg gccgaggcgc atggcggcga tgtcgacgga ctggccgcgc
     1441 atgtcatcgc tgcccgtcgc gcctcgaacg gaccgatcct ctatctctcg ggtgcggaca
     1501 ccgcgggcaa tcttcagggc cagcttgccg ccgccggttt cgactgccgt cgcgtggtgc
     1561 tctatgacgc ggttccggcc agagaccttg gcgcggcagc gactgcgctg cgccagcaca
     1621 aggccgatgc cgtcctcctc tattcgccgc gcagcgcccg gatctggtca agcctggcca
     1681 aggctgcggg actgagcgca gaagcggcgg ccgtgccaag cctctgcctg tcgcgcaatg
     1741 tggcggccgc ccttcccgag ggttgcaatg ccctcgtcgc ggaaaagccc gaaaacgccg
     1801 cgatgctgcg cctgcttgaa gagttgcacc gaacccgtta ggcctctgcc atgaccgaca
     1861 agcaaaagac tgacgacaac gatccgatca agccgcaggt catcgacctc aacgccgagg
     1921 aagtgacggt ggacgacgag ccgcgcccgg ccgatccgcc gccgggtcag tctgcgccc
//
LOCUS       DBNGVR010000100         3651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_429361, whole genome shotgun sequence.
ACCESSION   DBNGVR010000100 DBNGVR010000000
VERSION     DBNGVR010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_429361"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC16A0_02240"
     CDS             <1..243
                     /locus_tag="AC16A0_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR3104178.1"
                     /translation="RAMEIIAEIEQAPRGVYCGAIGYLGFNGHADLNIAIRTAQFCNG
                     VARLQGGGGITARSSPAAEYEESLTKIRRILEAFAA"
     gene            240..800
                     /locus_tag="AC16A0_02245"
     CDS             240..800
                     /locus_tag="AC16A0_02245"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3104179.1"
                     /translation="MILIVDNYDSFVHNVARYFAELGEATRVVRNDAVTEADLKARAI
                     VISPGPCTPQEAGQSIDIVRRFSGRIPILGICLGHQVIGEVFGGLVRRARRPMHGDSS
                     EINHSGTEIFAGLPQRFSAGRYHSLIVDLAGRDTPLEVTATSDEGEIMGLRHKSHPTF
                     GLQFHPESILTEGGYDMLRNFLKIAA"
     gene            797..1642
                     /locus_tag="AC16A0_02250"
     CDS             797..1642
                     /locus_tag="AC16A0_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3104180.1"
                     /translation="MSAHWFNGAIGQGPIALDPRDRGLNLGDGLFETILAVNRVPLWA
                     NMHLARMQSAANELGIPFNRAAVDTGIAALLDRAGDGHQVLRVTLTRGAGARGLAGTG
                     AVPALLITCDAFDAGLMFQPAALLTSTIRRSPESPATRLKTLSYIDNIFAAREAAARA
                     MDDALMLTTGDRVACSTIANIFLTKGRKLITPARDQGILTGVTRQALIAAAHHAGYDV
                     EERAVKPAELYKADAVFLTNSLRLIRPVKSLDQQPLNMADLTLLADGLCETARMQCGR
                     DPRLI"
     gene            1691..2341
                     /gene="fsa"
                     /locus_tag="AC16A0_02255"
     CDS             1691..2341
                     /gene="fsa"
                     /locus_tag="AC16A0_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR3104181.1"
                     /translation="MKFFVDTAEVNEIRELADIGLLDGVSTNPSLIAKSGRQFKEVIA
                     EICSIVSGPVSAEVVSLDYAGMMREAAELKKIAKNVTIKVPLTIDGLKACKALTSEGT
                     MVNVTLCFSANQALLAAKAGASFISPFVGRLDDIHLDGTELIAEIRQIYDNYAFDTEI
                     LAASLRTPLHVKQVALIGADVATMPPAVIKGLVKHPLTDKGIEQFMADWKKTGQSL"
     gene            2338..2637
                     /locus_tag="AC16A0_02260"
     CDS             2338..2637
                     /locus_tag="AC16A0_02260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3104182.1"
                     /translation="MILEHAILYVRPGQGPAFEQALKEALPLISATPGFIDLEVRPCL
                     EAEGRYLLLVRWRRLEDHTVGFRGSDRYPPWRAKLHHFYDPPPRVEHYGAAAVSA"
     gene            2683..3618
                     /locus_tag="AC16A0_02265"
     CDS             2683..3618
                     /locus_tag="AC16A0_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR3104183.1"
                     /translation="MSGYATAFAPDAADAASRWLASLRSERRMSPATIEAYARDLGQF
                     AAFLADHLGGSPAISDLEGLTVSDFRAFLARRRTGGAVSRSLARQLSALRGFYRHAER
                     TGRFRNAAISALQSPKLPHAVPKPLSVAAARTVAEADALADANTPRWVVARDSAVLTL
                     LYACGLRISEALALTPAGARSNPLSITGKGNKTRLVPVIPAARMAIDDYLARCPLSLK
                     PHEPMFRGVRGGPLNARNIQLLIEGLRGALGLPDTATPHALRHSFATHLLGNGADLRV
                     IQELLGHASLSTTQIYTEVNRAHLLEQYRKAHPRP"
BASE COUNT          603 a         1391 c         1068 g          589 t
ORIGIN      
        1 cgcgccatgg agatcatcgc ggaaatcgag caggcgccgc gcggcgtcta ctgcggtgcc
       61 atcggctatc tcggcttcaa cggccatgcc gatctcaaca tcgccatccg caccgcgcag
      121 ttctgcaatg gcgtcgcgcg cctgcagggc ggaggcggca tcaccgcgcg ctccagtcct
      181 gctgccgaat acgaggagag cctcaccaag atccgccgca tcctggaggc cttcgccgca
      241 tgatcctcat cgtcgacaac tacgacagct tcgtccacaa cgtggcgcgc tacttcgcgg
      301 agctcggcga ggccacgcgc gtggtacgca acgacgcggt gaccgaggcg gacctgaaag
      361 ccagggccat cgtcatatcg cccggcccct gcacgccgca ggaggccggc cagtcgatcg
      421 acatcgtccg tcgcttctcc ggccgcatcc cgatcctcgg catctgcctg ggccaccagg
      481 tgatcggcga ggtctttggc gggctggtcc gccgcgcccg ccggcccatg catggcgaca
      541 gctccgagat caaccattcc gggaccgaaa tcttcgccgg cctcccgcag cgcttcagcg
      601 ccggacgcta tcattccctg atcgtcgacc tcgcgggccg cgacacgccg ctcgaggtga
      661 ccgccaccag cgacgagggc gagatcatgg gcctccgcca caaatcccac ccgactttcg
      721 gcctacagtt ccatcccgaa tcaatcctga ccgagggggg ctacgacatg ctgcgcaact
      781 tcctgaagat cgcggcatga gcgcccactg gttcaacggg gctatcggcc agggccccat
      841 cgccctcgat cccagggacc gcggcctcaa tctcggtgac ggcctcttcg agaccatcct
      901 ggcggtgaac cgcgtgccgc tctgggccaa catgcatctc gcccgcatgc agtccgccgc
      961 caatgaactg gggattccct tcaaccgcgc cgccgtcgac accggcatcg ccgcgctgct
     1021 ggaccgggcc ggcgacggcc accaggtcct gcgcgtcaca ctgacccgcg gggcgggtgc
     1081 gcgcggcctc gccggcaccg gcgcggtgcc cgccctgctc atcacctgcg atgccttcga
     1141 cgccggcctc atgttccagc ccgcggcact cctcacctcc accatccggc gcagtcccga
     1201 atccccggcc acgcgcctca agacgctctc ctacatcgac aacatctttg ccgcccgcga
     1261 ggccgcggcg cgcgccatgg acgatgcgct catgctcacg accggcgatc gggtggcctg
     1321 ctccaccatt gcgaacatct tcctgaccaa gggccgcaag ctcatcaccc cggcacgcga
     1381 ccaggggatc ctgaccggcg tcacccgcca ggccctgatc gcggcggccc atcacgccgg
     1441 ttacgacgtc gaggagcgcg cggtaaagcc ggcggagctc tacaaggccg atgccgtctt
     1501 ccttaccaac agcctgcgcc tgatccgccc ggtcaagtcg ctggaccagc agccgctcaa
     1561 catggccgac ctgacgctgc tggccgacgg cctgtgcgaa acggcgcgca tgcagtgcgg
     1621 ccgcgatccc cggttgattt gacgccgggc aggggttaat cgtccggcgc aagcaagagg
     1681 accccgccag atgaaattct ttgtcgatac cgccgaagtg aacgagatcc gcgaacttgc
     1741 cgatatcggc ctgctcgacg gggtctccac caatcccagc ctgatcgcca agtcgggccg
     1801 ccagttcaag gaggtgatcg ccgagatctg ctccatcgtt tccggcccgg tatcggccga
     1861 ggtcgtctcg ctcgattatg ccggcatgat gcgcgaggct gccgagttga agaagatcgc
     1921 caagaacgtc acgatcaagg tgccgctcac catcgacggg ctcaaggcct gcaaggcgct
     1981 gacctccgag ggcaccatgg tcaatgtcac cctgtgcttc tcggccaacc aggcactgct
     2041 tgccgccaag gccggtgcca gctttatctc gcccttcgtc ggccgcctcg acgacatcca
     2101 tctcgacggc accgaactca tcgccgagat ccgtcagatc tacgacaatt atgccttcga
     2161 taccgaaatc ctcgccgcct cgctgcgcac gccgctgcat gtcaagcagg tggccttgat
     2221 cggcgccgat gtcgccacca tgccgcccgc cgtcatcaag ggcctggtca agcatccgct
     2281 caccgacaag ggcatcgagc agttcatggc cgactggaag aagaccggcc agtcgctgtg
     2341 atcctcgaac acgccatcct gtacgtgcgg ccgggccagg gcccggcctt cgaacaggcc
     2401 ctcaaggaag cgctgccgct gatcagcgcc acgcccggct tcatcgatct cgaggtccgg
     2461 ccctgcctcg aggcggaagg ccgctatctg ctgctggtcc gctggcggag gctggaggac
     2521 catactgtcg gcttccgcgg ctccgaccgc tatccgccct ggcgcgccaa gctgcatcac
     2581 ttctacgatc ctccgcccag ggtggaacac tacggtgccg cggcggtctc ggcctgaact
     2641 ccgccaagaa accgtcaacc attcgggctt aacctcaagc ccatgtccgg ttatgccaca
     2701 gccttcgcgc ccgatgccgc cgatgccgcc agccgctggc tggccagcct caggtcggag
     2761 cgccgcatgt cgccggccac catcgaggcc tatgcccgcg acctcgggca gtttgccgcc
     2821 ttcctcgccg atcacctggg cgggtcgccg gccatctccg atctcgaagg cctcacggtc
     2881 tccgactttc gtgcctttct cgcccgccgc cgcacgggcg gcgcggtaag ccggagtctc
     2941 gcccgccagc tgtccgcgct gcgcggcttc taccgccacg cggagcgcac aggccgcttc
     3001 cgcaatgccg ccatctcggc cctgcagtcg cccaagctgc cgcatgcggt ccccaagccg
     3061 ctcagcgtcg cggcggcccg cactgttgcc gaggccgatg ccctggcgga cgcgaacaca
     3121 ccgcgctggg tcgttgcccg cgacagcgcc gtgctcacgc tcctctatgc ctgcggcttg
     3181 cgcatctccg aggcgctggc cctcaccccg gccggcgcgc gcagcaatcc gctctccatc
     3241 accggcaagg gcaacaagac ccgcctcgtg ccggtgatcc cggccgcgcg catggccatc
     3301 gacgattacc tggcgcgctg tcccctgagc ctgaagccgc atgagcccat gttccgcggc
     3361 gtgcgcggcg gacccctgaa cgcccgcaac atccagctgc tcatcgaagg cctgcgcggc
     3421 gcgctcgggc ttcccgatac ggcaacgccg catgcgctcc gccattcctt cgccacgcat
     3481 ctgctgggga atggcgccga cctccgcgtg atccaggaac tgctcggcca tgccagcctc
     3541 tcgaccacgc agatctacac cgaagtcaac cgcgctcatc tcctcgagca gtatcgcaag
     3601 gcacatccaa ggccatgaaa cggatcgctc tcgcactcgc tgccatcctc t
//
LOCUS       DBNGVR010000101         6958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2197229, whole genome shotgun sequence.
ACCESSION   DBNGVR010000101 DBNGVR010000000
VERSION     DBNGVR010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2197229"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="AC16A0_02270"
     CDS             complement(<1..1007)
                     /locus_tag="AC16A0_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR3104184.1"
                     /translation="MTLVFPRRQAEAQAFLDANPDIQSVHVVWTDICGVARGKILRRD
                     EVIPAWKDGRFMPISALVLDVTGQDVPETGLVFDEGDRDMLMWPVPGSLQRIPWAEEP
                     SAQYIASICDLDGTPHYFDPRSVLEKIVTRFKSELNMTPVGAVELEFYLMDREAALAG
                     RPTAPKSLINEFKPQHYQAYYLQDLDDFAPFFKDLYAFCEAQGLPAKTLISEYSPGQM
                     EIVLRHRADVLKACDEAIMLKRLIKACAEKHGLAATFMAKPYSEWTGSGMHIHISLAD
                     EDGNNLFAAEDPLQNELLLHSLGGLKAAMAESMLIFAPNANSYRRFRRNSYAPVSASW
                     G"
     gene            complement(1021..2376)
                     /locus_tag="AC16A0_02275"
     CDS             complement(1021..2376)
                     /locus_tag="AC16A0_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3104185.1"
                     /translation="MPRTISADAIDAMLAREKALFVQRNPVSKQLAEDTAKTWLRGVP
                     MHWMVDWGMPFPLFVAKAQGVDVTDADGNNYIDFCLGDTGAMFGHSPKPVVAVLQREG
                     ANGFTTMMPSTDAAAVGKLLQDRFGLPCWQVTATASDANRAVIKWCRAITGRNKILVF
                     NHCYHGAVDDTYVTIDGGTPHADPALIGEVRDLTQFTKVVEFNDIPALEAALAPGDVA
                     CVLAEPIMTNVGMVLADDGYHAALREITRRTGTLLIIDETHCMSSGPGGYTGEYGLDP
                     DGLVLGKPIAGGIPAAVYGFSRPVADRIAAFLETRTPGHSGIGTTLAGSKIQLALMRA
                     VLETYFTQEAFAPLIALAKKLEEGIADVIIKHSLPWHVVRVGARVEFMCTPKPPRNGG
                     EAAQMIHRPIDVALHHYLLNRGVIVTPFHNMMLICPATTGAHVNRLVEGLDACVTELM
                     S"
     gene            2503..3351
                     /locus_tag="AC16A0_02280"
     CDS             2503..3351
                     /locus_tag="AC16A0_02280"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HIR3104186.1"
                     /translation="MSRTVFVNGDYLPESEGKISIFDRGFLFADGVYEVTAVVNGKLV
                     DYGPHMERLDRSLRELRMAWPCSKDELKAMHLEMVKRNALTEGIIYMQVTRGAADRDF
                     KFPKESKSTLIAFTQAMKLVDNPNAVTGVKVVTTPDIRWARRDIKTVMLLAPVLGKQD
                     AYEKGAHEAWMVEGGVVTEGTSSNAYIVKDGKVITRGLSNSILAGCTRRALFRLAQDH
                     GIVIEERSFTPDEAYAADEAFLTSASQFVMPIVEIDGRRIGGGQPGPVVRKLRELFLQ
                     EAASAG"
     gene            complement(3338..4999)
                     /gene="betA"
                     /locus_tag="AC16A0_02285"
     CDS             complement(3338..4999)
                     /gene="betA"
                     /locus_tag="AC16A0_02285"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HIR3104187.1"
                     /translation="MTPATYDFIIVGAGSAGCVMADRLSADGKNSVLLLEFGGSDRGP
                     FIQMPSALSYPMNMNLYNWGFETDPEPHLGGRRLATPRGKVVGGSSSINGMVYVRGHA
                     RDFDTWEEMGAKGWGFRHVQPYFKRMEHSHGGEDGWRGTNGPMHVTRGARSNPLYQAF
                     IEAGRQAGYPVTQDYNGHQQEGFGPMEMTVHRGVRWSAANAYIRPALKRPNMKLETYA
                     LAHRILLDGRRAIGVEYDVAGELKQAHARREVIIAASSINSPKLLQLSGIGAPETLKA
                     AGIDVVHALPGVGENLQDHLEIYFQLKSKEPVTLYSKLNWFSKGMIGLEWMLFGTGLG
                     ATNHFESCGFIRSRAGIEYPDIQFHFLPAAMRYDGKAAFNNHGFQVHVGPMRSKSRGF
                     VRIRSKDPRESPRILFNYLSHPDDLVEWRSCIRLTREIMAQPAMERYAGAEIQPGKVV
                     KTDDQIDAFIREHCESAYHPCGTCKMGSPQDPMAVVDAECRVIGIANLRVADSSIMPQ
                     VTNGNLNAPTLMIGEKASDNILGRDPLPPSNQEPWINPDWAVSQR"
     gene            complement(4996..5847)
                     /locus_tag="AC16A0_02290"
     CDS             complement(4996..5847)
                     /locus_tag="AC16A0_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR3104188.1"
                     /translation="MPTIAERLRLQSAELTAAERKLMAALFSNYPMAGLASITEFARE
                     ADVSTPSVLRLAKKLGFSGFPAFQEELRAELSAQLQNPIAKQERWSTDAPDAHILNRF
                     AVAAAENLSGSLRLLDHRVFDQVVGLLNDRKRRIHCGGGRITHAIAGYLAMHLQMARP
                     HVSLIPSAASHWPQYALDLGKGDVVVLFDIRRYDARMLEFAASAKERGARIVLITDQW
                     ISPIARLAVHSLPLRIEAPSPWDSNMVPLFVAETLVAATVAASWPATQARIRELESLS
                     TPGRRSK"
     gene            5944..6693
                     /locus_tag="AC16A0_02295"
     CDS             5944..6693
                     /locus_tag="AC16A0_02295"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR3104189.1"
                     /translation="MGAVLLDTEESPFILVNGSGQSPYVLVCEHASNLLPDRLGTLGL
                     EDAELLSHIAWDIGAEKVARRLSQLMDAPLALQRYSRLAYDCNRPPDMPSAMPDISEV
                     TPIPGNRNLTPDQRLARTREIYRPFHEGLADLLDLRAAEKMTSIVVSIHSFTPVFKGK
                     PRKVELGILHDRDTRLSSKLIKSFPNIEARLNEPYGPKDGVMHTLNLHAAPRGLQHAM
                     IEIRNDLIATDRGQDEWAQRLSVPLIQAATR"
     gene            6705..>6958
                     /locus_tag="AC16A0_02300"
     CDS             6705..>6958
                     /locus_tag="AC16A0_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HIR3104190.1"
                     /translation="MATGMLTLEQLKKEVAAGTIDTVVAALVDMQGRLMGKRFHAQYF
                     VDGAYEETHGCNYLLAVDVAMEPVPGYKASSWEKGYGDFVL"
BASE COUNT         1258 a         2118 c         2336 g         1246 t
ORIGIN      
        1 atgccccacg aggccgagac cggcgcatag gagttgcggc ggaagcggcg gtaggaattg
       61 gcgttgggtg cgaagatcaa cattgattct gccatcgccg ccttgaggcc gccgagcgaa
      121 tgcaggagaa gttcgttctg caacgggtct tccgccgcga agaggttgtt gccgtcctca
      181 tccgccagcg aaatgtggat gtgcatgccc gaacccgtcc actcggaata gggcttggcc
      241 atgaaggtgg cggcaagccc gtgcttctcg gcacaggcct tgatcagccg cttcagcatg
      301 atggcctcgt cgcaggcctt cagaacgtcg gcgcggtggc gcagcacgat ctccatctgg
      361 cccggcgaat actccgagat cagtgtcttg gccggaaggc cctgcgcctc gcagaaggca
      421 tagagatcct tgaagaaggg cgcgaagtcg tcgaggtcct gcaggtagta ggcctggtaa
      481 tgctgcggct tgaactcgtt gatcagggat ttcggcgccg tgggccggcc ggcgagcgcg
      541 gcctcgcggt ccatcaggta gaattcgagc tcgaccgccc ccaccggcgt catgttgagc
      601 tcgctcttga agcgggtgac gatcttctcc agcacgctgc gcgggtcgaa gtagtggggc
      661 gtgccgtcga gatcgcagat cgaggcaatg tattgagccg aaggttcttc cgcccaggga
      721 atgcgctgca ggctgccggg caccggccac atcagcatgt cgcggtcacc ctcgtcgaag
      781 acgagaccgg tctccggcac gtcctggccc gtcacgtcga ggacgagcgc cgaaatcggc
      841 atgaagcggc catctttcca ggcggggatc acctcgtcgc ggcgcaggat cttgccgcgg
      901 gcgacgccgc agatatcggt ccagacgacg tgcacgctct ggatgtcggg attggcgtca
      961 agaaaggcct gggcttcggc ctgccggcgg gggaatacga gagtcatctt tgtctccggg
     1021 tcagctcatc agttccgtca cgcaggcatc gagtccctcg acaagccggt tgacgtgcgc
     1081 cccggtggtc gcggggcaga tgagcatcat gttgtggaag ggcgtaacga tcacgccgcg
     1141 gttgagaaga tagtgatgca gcgccacgtc gatcgggcgg tggatcatct gcgcggcctc
     1201 gccgccgttg cgcggtggtt tcggcgtgca catgaattcc acgcgagccc cgacacggac
     1261 gacgtgccac ggcagggaat gtttgatgat gacatccgcg attccctcct cgagcttctt
     1321 cgcaagcgcg atgaggggcg cgaaagcctc ctgtgtgaag taggtctcga gcacggcgcg
     1381 catgagcgcg agctggatct tcgaccccgc gagcgtggtg ccgatgccgg aatggccggg
     1441 tgtgcgggtc tcgagaaacg ccgcaatgcg gtcggctaca ggccgggaga agccatagac
     1501 ggcagcggga atgccgccgg caatgggctt gcccagcacg agcccgtcgg gatcgaggcc
     1561 gtattcgccc gtgtagccgc cgggaccgga cgacatgcaa tgcgtctcat cgatgatcag
     1621 cagcgtgccg gttcgcctgg tgatctcgcg cagggccgcg tgatagccgt cgtcggccag
     1681 caccatgccg acattggtca tgatcggttc ggccagcacg caggcgacat cgcccggcgc
     1741 gagcgcggct tcgagcgcgg ggatgtcgtt gaactccacc accttggtga actgcgtcag
     1801 gtcgcgtacc tcgccgatca gggcgggatc ggcatgcggc gtgccgccgt cgatggtgac
     1861 ataggtgtcg tccaccgcgc cgtgatagca atgattgaag acgaggatct tgttgcggcc
     1921 ggtgatggcg cggcaccact tgatgacggc gcggttggcg tcagacgccg tggcggtcac
     1981 ctgccagcag ggcaagccga agcggtcctg cagcagtttt ccgacggcgg ccgcatcggt
     2041 cgaaggcatc atggtggtga agccattggc gccttcgcgc tgcaggaccg ccaccaccgg
     2101 cttgggcgaa tggccgaaca tggcgccggt gtcgcccagg cagaagtcga tgtagttgtt
     2161 gccgtcggcg tcggtgacat cgacgccctg cgcctttgcc acgaagagcg ggaagggcat
     2221 gccccagtca accatccagt gcatcggcac gccgcgcagc caggtcttcg ccgtgtcttc
     2281 cgcgagctgt ttcgataccg gattgcgctg gacgaaaagg gccttctcgc gggccagcat
     2341 ggcatcgatg gcgtcggcgg aaatggtgcg cggcatgata tccttgaaga cagttgcgac
     2401 ggcctgtctc ctagcacgcc gggagggggg cttcacaacc cccatggatt tgaccggggg
     2461 aatctgatta acccagccgg ctgtcttcga ggagcccggc ccatgtcccg caccgtcttt
     2521 gtgaatggcg attatcttcc tgaatcggaa ggaaaaatct cgatcttcga ccgcggcttc
     2581 ctgttcgccg atggggttta cgaggtgacg gccgtggtga acggcaagct ggtggattac
     2641 gggccgcaca tggagcggct tgaccgctcg ctgcgcgaac tgcgcatggc ctggccctgc
     2701 agcaaggacg aactgaaggc catgcatctg gagatggtga agcgcaacgc gttgaccgaa
     2761 ggcatcatct atatgcaggt cacgcgcggc gcggctgacc gcgacttcaa gtttccaaag
     2821 gaatcgaaat ccacgctcat cgcattcacc caggcgatga agctggtcga caatcccaat
     2881 gccgtgacgg gcgtgaaggt cgtgaccact cccgatatcc gctgggcgcg gcgcgacatc
     2941 aagacggtca tgctgctggc gccggtgctc ggcaagcagg atgcctacga gaagggcgcg
     3001 catgaagcct ggatggtgga aggcggtgtc gtcaccgagg gcacatcgtc caacgcctat
     3061 atcgtgaagg acggcaaggt gatcacccgc ggcctgtcca attccatcct cgccggctgc
     3121 acgcggcgcg cgctgttccg gctcgcgcag gaccacggca tcgtgatcga ggaacgcagc
     3181 ttcacgccgg acgaagccta tgcggcggac gaggccttcc tcaccagcgc ctcgcaattc
     3241 gtcatgccca tcgtggagat cgacggccgg cgcatcggcg gcggccagcc cggcccggtg
     3301 gtgcgcaagc tgcgggagct tttcctgcag gaggcggcta gcgctggctg accgcccagt
     3361 cgggattgat ccagggttcc tggttcgagg gcggcagcgg atcacggccg aggatgttgt
     3421 cggacgcctt ctcgccgatc atcagcgtgg gcgcattgag attgccgttg gtgacctgcg
     3481 gcatgatgga ggaatcggcg acccgcaggt tcgcgatgcc gatcacgcgg cattcggcgt
     3541 ccaccaccgc catgggatcc tgcggcgaac ccatcttgca ggtgccgcag gggtgatagg
     3601 cgctctcgca gtgctcgcgg atgaaggcgt cgatctgatc gtccgtcttg acgaccttgc
     3661 ccggctggat ctcggcgccg gcgtagcgct ccatcgcggg ctgggccatg atctcgcggg
     3721 taagcctgat gcagctgcgc cactcgacga gatcgtcggg atgggagaga tagttgaaca
     3781 ggatccgcgg actctcgcgc gggtccttgc tccggatgcg aacgaagccg cgcgatttcg
     3841 agcgcatcgg cccgacatgg acctgaaagc cgtggttgtt gaaggcggcc ttgccgtcat
     3901 agcgcattgc ggcgggcagg aagtggaact ggatatcggg atactcgatg ccggcgcgcg
     3961 agcggatgaa gccgcaggat tcgaagtggt tggtggcgcc cagacccgtg ccgaagagca
     4021 tccactccag cccgatcatg cccttcgaga accagttcag cttcgaatag agggtgaccg
     4081 gctccttgga cttcagctgg aaatagatct cgaggtggtc ctgcaggttt tcgcccacgc
     4141 cgggcagggc gtgcaccacg tcgatgccgg cagccttcag cgtctccggt gcaccgatgc
     4201 ccgagagctg cagcagcttc ggcgaattga tgctggaggc ggcgatgatc acctcgcgcc
     4261 gcgcatgggc ctgcttcagc tcgccggcca cgtcatactc caccccgatg gcgcggcgtc
     4321 catcgagcag gatccggtgc gccagggcat aggtctcgag cttcatgttg ggccgcttca
     4381 gtgccggcct gatgtaggca ttcgcggccg accagcgcac gccccggtgc accgtcattt
     4441 ccatggggcc gaagccctcc tgctggtggc cgttgtagtc ctgcgtcacc ggatagcccg
     4501 cctggcggcc agcctcgata aaggcctggt agagcgggtt gctgcgtgcg ccgcgcgtga
     4561 catgcatggg cccgttggtg ccgcgccagc cgtcttcgcc gccgtgggaa tgctccatgc
     4621 gcttgaaata gggttgcacg tgacggaaac cccagccctt tgcgcccatc tcctcccagg
     4681 tgtcgaaatc ccttgcatgg ccgcgcacat agaccatgcc attgatcgaa gatgagccgc
     4741 ccaccacctt gccccgcggc gtggccaggc gccggccgcc gagatgcggc tcgggatcgg
     4801 tttcgaagcc ccagttgtag aggttcatgt tcatcggata ggacagcgcc gagggcatct
     4861 ggatgaaggg gccgcggtcg ctgccgccga attccagcag caggacgctg ttctttccgt
     4921 cggccgagag ccggtccgcc atcacgcagc cggccgagcc tgcgccaacg atgatgaagt
     4981 cgtaggtcgc aggcgtcatt tgctgcggcg tccgggggtc gacagggact cgagttcgcg
     5041 gatgcgggcc tgggttgcgg gccatgacgc cgccaccgtg gccgcgacca gagtctcggc
     5101 gacgaagagg ggaaccatgt tcgagtccca gggggagggt gcttcgatac gcaacggaag
     5161 ggaatgcacg gcaagtctcg cgatcgggga tatccactga tcggtaatca gcacgatccg
     5221 ggcgccgcgc tctttggcgg aagcggcaaa ttccagcatg cgggcgtcat agcggcggat
     5281 atcgaagagc accacaacat cgcccttgcc gaggtcgagg gcatattgtg gccagtgcga
     5341 cgccgcagaa gggatcagcg acacatgcgg ccttgccatc tggagatgca tggcaaggta
     5401 accggcgatg gcatgggtga tgcgaccgcc gccgcagtgg atgcggcgct ttcggtcatt
     5461 gagcaggccg acgacctgat cgaagacccg gtggtcgagc agccggagcg agcccgagag
     5521 gttttccgcc gcggcgacgg cgaagcgatt caggatatgg gcatcgggcg cgtcggtcga
     5581 ccagcgctcc tgcttggcga tggggttctg cagctgtgcc gagagttcgg cgcgcagttc
     5641 ttcctgaaag gcgggaaaac cgctgaagcc cagcttcttg gccaggcgca ggacactggg
     5701 cgtcgagacg tcggcctcgc gcgcgaactc ggtgatgctg gccagtcccg ccatgggata
     5761 gttcgaaaag agcgccgcca tcagcttccg ctcggcggcg gtcagctcgg cggactggag
     5821 gcgcaggcgt tctgcaatcg ttggcatggc ggttttccgg attccgccgc actgtaacga
     5881 aattgacagt gattgcacaa tctgaaatca tccattcacc ttcctgccgt tccggagtct
     5941 ttcatggggg ccgtcctcct cgacactgaa gaaagccctt tcattctcgt gaacggatcc
     6001 ggccagtcac cctatgtgct ggtctgcgaa catgcgtcca acctgctgcc agacaggctg
     6061 ggaacgctcg ggctcgaaga tgccgaactg ctgagccaca tcgcctggga catcggtgcc
     6121 gagaaggtgg cgcgccggct gtcgcagctc atggatgcgc cgctggccct gcagcgctat
     6181 tcacggcttg cctatgattg caatcgcccg ccggacatgc cgagcgccat gccggatatc
     6241 agcgaagtca cgccgatccc cggcaaccgc aacctgacgc cggaccagcg ccttgcccgc
     6301 acgcgcgaga tctaccggcc gtttcacgaa gggcttgccg atctgctgga tctccgcgca
     6361 gcggagaaga tgacgtccat cgtcgtttcg atccacagct tcacgccggt cttcaagggc
     6421 aagccgcgca aagttgaact gggaatcctg catgaccgcg acacccgcct gtcgtccaag
     6481 ctcatcaaga gttttccgaa tatcgaggcc cgtctcaacg agccctatgg gccgaaggac
     6541 ggcgtcatgc acacgctcaa cctgcacgcc gcgccgcgcg gcctgcagca cgccatgatc
     6601 gagatccgca atgatctgat cgccaccgac cgcgggcagg acgaatgggc tcagcgcctg
     6661 tcggttccgc tcattcaagc cgcaacaaga taggggacaa gacgatggcc acgggcatgc
     6721 tgacgcttga acagctgaag aaggaagttg ccgccggaac gatcgacacg gtcgtcgcgg
     6781 cactggtcga catgcagggg cggctgatgg gcaagcgctt ccatgcgcag tacttcgtgg
     6841 atggcgccta tgaggaaacc catggctgca actacctgct ggccgtcgat gtcgccatgg
     6901 agccggtgcc gggctacaag gcctcgagct gggaaaaggg ctatggcgat ttcgtgct
//
LOCUS       DBNGVR010000102         1566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3966035, whole genome shotgun sequence.
ACCESSION   DBNGVR010000102 DBNGVR010000000
VERSION     DBNGVR010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3966035"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="AC16A0_02305"
     CDS             <1..211
                     /locus_tag="AC16A0_02305"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR3104191.1"
                     /translation="MIGVVAALREIETKEGREIPMRPDHGHVIVDDLGKRVTPGYSCI
                     GRLKGLAELRGVMRTVDAFRSGRLH"
     gene            223..1404
                     /locus_tag="AC16A0_02310"
     CDS             223..1404
                     /locus_tag="AC16A0_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3104192.1"
                     /translation="MRAFLTFLAAYVLSQFYRSFLAVIAPELARELALDPQALGNMQA
                     FWILGFVAMQFPVGWALDTQGPRRTVAAIMLAAVAGAALFARATSAFDLNLAMVLIGV
                     GCSAIYMGAIYVFGRVNRPDRFALLCSWLLGIGTAGNLLAASPLAFAAEMIGWRVSML
                     ILAALTAASALSVVLLIRDPPRLSALGGDGFFAGIGEILATRALWPLLPLTAIGYSVI
                     LAERGLWAGPFFSEVYGLSPVERGNALLVMAAAMSAGAMIYGPLDKWLGTRKWVVGAG
                     SAVTAACFVLLGTRELSLTGATIAMAALGGFGMTYGVLMAHGRSFVPDRLLGRGITLL
                     NVLFIGGAGLLQPLSGAFMTSMAGQPPAEAFATLHLGFGLLLLLALLVYLFSREHRAE
                     T"
BASE COUNT          188 a          556 c          507 g          315 t
ORIGIN      
        1 catgatcggc gttgtggcgg ccctgcgcga gatcgagacg aaagagggcc gggagatccc
       61 gatgcggccc gaccatggcc atgtcatcgt cgacgacctc ggcaagaggg tgacccccgg
      121 ctattcctgc attggccgcc tcaaggggct ggcggaactg cgcggcgtga tgcgcaccgt
      181 cgacgccttc cgcagcggcc gcctgcactg aggcatggcg gcgtgagagc cttcctcacc
      241 ttcctcgccg cctatgtgct gagccagttc tatcgctcct tcctcgcggt gatcgcaccc
      301 gaactggcgc gcgaactggc gctcgatccg caggcgctgg gcaacatgca ggccttctgg
      361 atcctgggct tcgtggccat gcagttcccg gtgggctggg ccctcgacac gcagggaccg
      421 cgccgcaccg ttgccgcgat catgcttgcc gccgtcgcgg gtgcggcgct cttcgcgcgc
      481 gcgacatctg ccttcgatct caaccttgcc atggtgctga tcggcgtcgg ctgcagcgcc
      541 atctacatgg gcgccatcta tgtcttcggc cgggtcaatc gcccggatcg tttcgccttg
      601 ctctgctcgt ggctgctcgg catcggcacg gcgggcaatc ttctggcggc ctcgccgctg
      661 gccttcgccg ccgagatgat cggctggcgc gtgtcgatgc tgatccttgc ggcgctgacc
      721 gcggcctctg ccctctccgt cgtcctgctc atccgcgatc cgccgcgcct atcggcgctc
      781 ggtggcgacg gtttcttcgc cggtatcggc gagatcctcg cgactcgcgc gctctggccc
      841 ctgctgccgc tgaccgcgat cggctattcc gtcatcctcg ccgaacgcgg cctctgggcc
      901 ggccccttct tctccgaggt ctatggcctg tcgcccgttg agcgcggcaa tgcgcttctg
      961 gtcatggcgg cggccatgtc ggccggcgcc atgatttacg gaccgctcga caaatggctc
     1021 ggcacgcgca aatgggtggt gggcgccggc agcgcggtga cggcggcctg cttcgtgctg
     1081 cttggcacgc gggaactgtc gctgacgggt gcgacgatcg cgatggcggc actcggcggc
     1141 ttcggcatga cctacggcgt gctcatggcc catggccgca gcttcgtgcc agaccgcctg
     1201 ctgggccggg gcatcacgct gctcaacgtg ctcttcatcg gcggcgccgg gctgctgcag
     1261 cccctgagcg gcgccttcat gacctcgatg gcggggcaac cgccggccga ggccttcgcg
     1321 acgctgcatc tcggcttcgg gctgcttctc ctgctggcgc tcttggtcta tctcttttcg
     1381 cgcgaacatc gcgcggagac ctgatccatg ctcttcgacc gcagcaagtc ctgtctcgtc
     1441 gtcatcgacg tgcagcaata cttcctcgac aagctgccgc tggactggcg tgcaccactg
     1501 gtccagcgca tcgcctggct catgcggctt gccgctgcgc tcgacattcc ggtcatcgcc
     1561 acggcc
//
LOCUS       DBNGVR010000103         2895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4359156, whole genome shotgun sequence.
ACCESSION   DBNGVR010000103 DBNGVR010000000
VERSION     DBNGVR010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4359156"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="AC16A0_02315"
     CDS             <1..1164
                     /locus_tag="AC16A0_02315"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR3104193.1"
                     /translation="GIEKFLDGQGAIYTASLADPSEHKTEPAQMSIDIRVQAALIDEL
                     TQSIAKFRAIAGGGIVMDITTGEVLALASLPDFNPNDENKSFSKDQMNKMTSGVFELG
                     SVIKAVTFAMAFDYGTATLESRYDARFPLVIGNARINDYHAKRAILSVPDIFVNSSNI
                     GTARMALDVGLENHQEFLRRVGLFDRLVTELPESARPLLPKRWSKLATATAAFGHGFA
                     VQPLQGAAVVAGLLNGGYLMTPTFLRRSPEEAMAMAKQIVKPTTSETMRYLFRLNAAD
                     GTASKADVIGYRVGGKTGTAEKVVNGRYSKEKRLTSFIGAFPMDDPRYLVLVMLDEPK
                     PTPETYGFATSGWNAVPAAAKIIERIAPMLGVEPRFTAEDLEKLEKQKKAKKG"
     gene            1176..2630
                     /locus_tag="AC16A0_02320"
     CDS             1176..2630
                     /locus_tag="AC16A0_02320"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR3104194.1"
                     /translation="MPLSRLIGEDAAVPQGAGSLAIQGLTSDSRAVKPGFLFAALPGS
                     SVDGARFIPQAIKAGAVAVLAGEGVAADGAAVVAARNPRQAFAHMAARFYGRQPDLIA
                     AVTGTNGKTSVASFVREIWTAMGFRAASLGTVGVVAPSGIIELQHTTPDPVTLHEIAA
                     RLAEDHVEHLALEASSHGLDQYRLDGLRISAGAFTNITRDHLDYHPSFEAYFDAKMRL
                     FEELLPLGAPAVVNMDTPHGSEVEKRCRARGLSVFTVGEQGTGLRLIRSERDGFGQRL
                     EIQGPARRHAVLLPLVGGFQVSNALVAAGMVVATGGEEVLAMHCLESLKGAKGRLELV
                     ARASSGAPVFVDYAHTPDALDNAIQSLRPYVTGRLAVDFGCGGDRDKGKRPQMGAVAA
                     RLADRVYVTDDNPRSEDPAAIRREIMAASPGAVEIGGRAEAIRAAVAGLSSGDILLVA
                     GKGHEEGQKIGATVIPFSDHEAVKAAIAGVDDDG"
     gene            2620..>2895
                     /locus_tag="AC16A0_02325"
     CDS             2620..>2895
                     /locus_tag="AC16A0_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013396.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR3104195.1"
                     /translation="MMAEQALWTPQDLVAATNGRIDGNVTQVLTGITIDSRAAKAGEI
                     FVAIKGERHDGHDFVAAALKAGAGLAMVSRATAEMRAAGPLLVVAEDP"
BASE COUNT          503 a          947 c          966 g          479 t
ORIGIN      
        1 ggcatcgaga aattcctcga tggccagggc gcgatctata cggcttcgct tgccgatccg
       61 agcgagcaca agaccgagcc cgcgcagatg tcgatcgaca tcagggttca ggccgccttg
      121 atcgacgaac tcacgcagtc gatcgcgaag ttccgcgcca tcgccggcgg cggcatcgtg
      181 atggatataa ccacgggcga agtgctggcc ctagcctcgc tgccggactt caatccgaac
      241 gacgagaaca agagcttcag caaggaccag atgaacaaga tgaccagcgg cgtcttcgag
      301 ctgggctcgg tcatcaaggc ggtcaccttc gccatggcct tcgactacgg cacggcaacg
      361 ctggagagcc gctacgatgc gcgcttcccg ctggtcatcg gcaatgcccg catcaacgac
      421 tatcatgcca agcgcgccat cctgtcggtg cctgacatct tcgtcaattc gtccaacatc
      481 ggcacggccc gcatggcgct cgacgtgggg ctcgagaacc atcaggaatt cctccgccgc
      541 gtcgggctct tcgaccggct ggtgaccgaa cttccggaaa gcgccaggcc gctgctgccc
      601 aagcgctgga gcaagcttgc cacggcaacg gccgccttcg gccatggttt tgccgtccag
      661 ccgctgcagg gtgcggccgt ggtcgcaggt cttctcaatg gcggatatct gatgacgccc
      721 accttcctgc ggcgcagccc ggaagaggcg atggccatgg ccaagcagat cgtcaagccc
      781 acgaccagcg agaccatgcg ctacctgttc cggctgaatg cggcggatgg cacggcttcg
      841 aaggccgacg tgatcggcta tcgcgtcggc ggcaagaccg gcacggccga gaaggtggtg
      901 aacgggcgct actcgaagga gaagcggctg acttccttca tcggggcctt cccgatggac
      961 gatccaaggt atctcgtgct ggtgatgctc gacgaaccga agccgacgcc cgagacctat
     1021 ggctttgcca cctcgggctg gaacgcggtt ccggcggccg ccaagatcat cgagcgcatt
     1081 gcgccgatgc tgggcgtcga accccgtttc acggccgaag acctcgagaa actcgaaaag
     1141 cagaagaaag cgaagaaggg ctgaccgccg tgaccgtgcc gctgtccagg ctgattggtg
     1201 aggatgccgc ggtgccgcag ggcgccggat cgcttgccat tcaggggctg acatccgaca
     1261 gccgggcggt gaagcccgga tttctgttcg cagccctgcc gggcagcagc gtcgatggcg
     1321 cacgcttcat tccacaggcg atcaaggctg gtgcggttgc cgtcctcgca ggcgagggcg
     1381 ttgcggctga cggcgccgcc gtcgtcgcgg cccgcaaccc gcgccaggcc ttcgcccata
     1441 tggctgcccg cttctacggc cggcagcccg atctcatcgc cgccgtgaca ggcaccaacg
     1501 gcaagacctc ggtcgcttcc ttcgtgcgcg agatctggac ggccatgggt tttcgcgccg
     1561 ccagccttgg tacggtcggt gttgttgcgc cctcgggcat catcgaactg cagcatacga
     1621 cgcccgatcc ggtgacgctg cacgagatcg ccgcgcggct cgccgaagat catgtcgagc
     1681 atctggcgct tgaagcctcg agccatggac tcgaccagta tcgcctcgat ggcttgcgca
     1741 tctcggccgg cgccttcacc aacatcacgc gcgaccatct cgactatcac cccagcttcg
     1801 aggcctactt cgacgcaaag atgcggctgt tcgaggaact gctgccgctg ggcgcgccgg
     1861 cagtggtcaa catggacacg ccacacggct ccgaggtcga gaagcgctgc cgcgcgcggg
     1921 ggctgagcgt cttcaccgtg ggagaacagg ggaccggcct caggctgatc cgctccgagc
     1981 gcgatggctt tggtcagcgg ctggagatcc agggcccggc ccggcgccat gccgtgctgc
     2041 tgccgctggt cggcggcttt caggtgtcca atgcgctggt ggcggccggc atggtcgtgg
     2101 cgacgggcgg cgaggaggtc ctcgccatgc attgcctgga atcgctcaag ggcgccaagg
     2161 gccggctcga actggttgcc cgcgccagca gcggcgcgcc cgtcttcgtc gattatgccc
     2221 atacgcccga tgcactcgac aatgccattc aatcgctccg gccctatgtc accggacgcc
     2281 tggccgtcga ctttggctgc ggtggtgacc gcgacaaggg caagcgtccg cagatgggtg
     2341 cggtggccgc gcgcctggct gaccgcgtct atgtgaccga cgacaatccc cgcagcgagg
     2401 atccggccgc catccggcgc gagatcatgg cggcaagccc gggcgccgtc gagatcggag
     2461 gacgggcaga agcgatccgc gccgctgtgg ccggcctgtc gtccggagac atcctgctgg
     2521 tggccggcaa gggccatgag gaggggcaga agatcggcgc gaccgtcatt cccttcagtg
     2581 accatgaggc ggtgaaggcc gccatcgcag gagttgacga tgatggctga acaggcgctg
     2641 tggacgccgc aggatctggt tgccgcaacg aacggccgga tcgacggcaa tgtcacccag
     2701 gtgctgaccg ggatcaccat cgattcgcgc gccgccaagg cgggcgagat cttcgtggcg
     2761 atcaagggcg agcggcatga tggccacgac ttcgtcgccg ctgccctgaa ggccggggca
     2821 ggtcttgcca tggtctcgcg cgccaccgcg gagatgcggg ctgccggtcc gctgctggtc
     2881 gtggccgagg atccc
//
LOCUS       DBNGVR010000104         4726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4359209, whole genome shotgun sequence.
ACCESSION   DBNGVR010000104 DBNGVR010000000
VERSION     DBNGVR010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4359209"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC16A0_02330"
     CDS             complement(<1..197)
                     /locus_tag="AC16A0_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104196.1"
                     /translation="MPLIIIALLPFLGALLPALLIRAGRNSCTWSAVVVTGTALIGLL
                     TRAPEVFSGGVVEEKLDWLPV"
     gene            495..1241
                     /locus_tag="AC16A0_02335"
     CDS             495..1241
                     /locus_tag="AC16A0_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3104197.1"
                     /translation="MLGEPARDVIVQQQHRYNAVAQRIEQQIRDGEIAAGSRILGERE
                     LAKLLGVSRVTLRRALGALRDRGVLVSDQAKGWFVAPPNVSEQNVLRSFSEMAAARGL
                     ACRSQVIRSSVRIAGFEEAEVLRLEAGAQVFELARVRFLAGEAVGIEESLVPLQRAPN
                     LPKADFSRQSLYVAMRDAGAGPAIADYVLSAASATAEQAKLLRVPVGTALLTTNAIAR
                     DVNGAPVELSKTFFRGDRYRFLTTLKAELR"
     gene            1238..2560
                     /locus_tag="AC16A0_02340"
     CDS             1238..2560
                     /locus_tag="AC16A0_02340"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3104198.1"
                     /translation="MTAGFLGIDVGLSGARATVVSSSGKLLAQARLDGATGAMPPARL
                     ARLVDQTIRLALKGRRLSVSSIAVAAFGPAPILLDGQGQVLAHLALFEGAGGESHGGA
                     VDDDLAERLRRFRRSSPRLYGKARQICDVTGYLVSRLTGQLAMDHVTAADYARLPLPR
                     RIALPRLAAGDQAAGSLAPAAARRLGLEAGVPVAVGTYDSTADLLAAGFGRQRRAVIV
                     LGSTMVLGALTAAPLRDPRLRSMPHLGPGWFSGGWTNFAGSSLALADALIAKGNGKDD
                     GAVPLVWPYFAGERAPVWAPHASGMIAGLRAGTTASAVRRGFVQGVALSAADIAYRLA
                     VETGPIRRWTVIGGGSRDEALMGELADALGATLDIVKGAADHLGPAVLAARSAGVELR
                     LPIGRRYRPRRAAHHAYGERLRIYRQVFAAVQPLLSGIRHVTGSGSRV"
     gene            2560..3549
                     /locus_tag="AC16A0_02345"
     CDS             2560..3549
                     /locus_tag="AC16A0_02345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3104199.1"
                     /translation="MTDTMRSARLVGIRQMQIDRLPVTPPQAGEIQVRIEACGVCATD
                     SRKYEIGVNDGHYPFNPGHEWLGEVSAIGSGVKGFRVGDRVYGDTYGGYADFATIPVD
                     PIDWSRGPLLLPRDLDRRRAIFIEPLADCIHAVRDQARLEPGQKLVVIAAGVMGLKII
                     ADAARLGCEVMAVEPLVPRQAAARTFGASLVTGPENWAEAAADWTGGRGVDAVILTIG
                     NPDLVMPCVRAARPGGRVVLFAGFGNRKEAVLDLNDIHYREIALIGSEWIGTPPNQRR
                     HRYDEALDFLLDARVPFEDLVTSTCGFATLEQALVARQGFTGFKIMFEPERES"
     gene            3546..4319
                     /locus_tag="AC16A0_02350"
     CDS             3546..4319
                     /locus_tag="AC16A0_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013916.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR3104200.1"
                     /translation="MMTPACIGADGRSVIIAIDHALYSWPCRGLEDRKSIISAANAAG
                     ADAIIASYGTIRDFRRDFGRVKPILKLDLTSVTLGGHYPLTDYVMAYGLDDAERLGVS
                     TVLNFIQLGAPFELEALRQSGRLAAECDRRGFTYLCEIMPVESAMFPDPEAPEAMVAA
                     CRTGQELGAHVIKTTMPVPSAEIAAAVAACNIPVVLAGGALAKDAAAYDAAISAALSA
                     GVAGVAIGRNAWGAADPAAAMQRFCRLIHGGTAKEVKRA"
     gene            4316..>4726
                     /locus_tag="AC16A0_02355"
     CDS             4316..>4726
                     /locus_tag="AC16A0_02355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104201.1"
                     /translation="MTVARDLDDRLAIVTGASSGIGRSVAMLLARRGARVVAVGRNRA
                     RLDELAALHDGIVPHIADLADPGSTDLVFHAATRLGAATILVCAAGLPGFTDRPIASQ
                     SFEAWRATMAVNLDSPFLMTRAIAPLIAAARWGRI"
BASE COUNT          754 a         1535 c         1601 g          836 t
ORIGIN      
        1 atgacgggaa gccagtccag cttttcctcc acgactccgc cggaaaacac ttcaggagca
       61 cgagtcagca ggccgatcag ggccgtccct gtcacgacca ccgccgacca tgtgcaggaa
      121 ttgcgccccg cgcgaatcag cagcgcgggc agaagcgcac cgagaaaggg caggagggca
      181 atgatgatca gcggcacggc gggctccaga ccggaagaac ttgcggatga ggatcacccg
      241 ggactttgct gacaaacgtc atcagcagca atgaataccg tcgccgctgc gatttctcca
      301 cacaggatgt ggcaggtacc gcgtttctaa ctgatctgta atctcgtcct agcctgcaag
      361 gcgtgggcgg atgcgcctgc gtgcttgggg cggggtcggc tgccacggga accgaggcga
      421 ttgcctacgc ccatatccgg cgaagtgtcc tgctggatct tgactggtga gaatctggta
      481 ttactctggt ctacatgctc ggggagccgg ccagagacgt cattgtccag cagcagcatc
      541 gctacaatgc ggtggcgcag cggatcgaac agcagattcg cgacggcgag attgctgccg
      601 ggagccgcat tctgggcgaa cgggagcttg ccaagctgct cggcgtcagc agggtcacct
      661 tgcggcgggc cctcggcgcg ttacgcgacc gcggggtgct cgtatcggac caggccaagg
      721 gctggttcgt ggcgccgccg aacgtcagcg agcaaaatgt cctgcgcagc ttttccgaga
      781 tggctgctgc acgcggactt gcctgcaggt cccaggtgat ccggagttcc gttcggatcg
      841 ctggtttcga ggaagcggag gtgctgcgcc tcgaggccgg cgcgcaggtc ttcgaacttg
      901 cacgggtgcg ctttctcgca ggcgaagcgg tgggcatcga ggaaagcctc gtgccgctgc
      961 agcgcgcgcc caacctgccg aaagccgact tttccaggca gtcgctctat gtggccatgc
     1021 gtgacgcggg cgccggcccc gccatcgcgg actatgtcct ttcggccgcg tcggccacgg
     1081 cggaacaggc gaaactgttg cgggtgccgg tgggaacggc cctgctgacc accaatgcca
     1141 tcgcccgcga tgtcaatggt gcacccgtcg aactgagcaa gacattcttc cgcggcgacc
     1201 gctaccggtt tctgaccaca ctgaaggccg agctcagatg acagcgggct ttctgggaat
     1261 cgatgtggga ctgtcgggtg cgcgggctac cgtggtttcg tcgagcggca agctgctggc
     1321 gcaggcaagg ctcgacggtg cgacgggagc gatgccgcct gcacgtctgg cccgcctggt
     1381 ggatcaaacg atccggcttg ccctcaaggg ccggcgactg tccgtctcgt ccattgcagt
     1441 ggcggccttc ggaccggcac ccattctgct cgatggtcaa ggacaggtcc tggctcacct
     1501 tgcgcttttc gaaggcgccg gaggcgaaag ccacggcgga gcagttgatg acgatctcgc
     1561 ggagcggctg cggcggttcc ggcgcagctc gccccggctc tacggcaagg cccgccagat
     1621 ctgcgacgtc accggctatc tcgtatcgcg gctgacgggg cagcttgcca tggaccatgt
     1681 gaccgctgcc gactacgccc ggctgccgtt gccgcggagg attgccttgc cacggctcgc
     1741 cgccggggat caggccgccg gatcactggc ccccgcagct gcgcgccgcc ttggtctgga
     1801 ggcgggcgtc ccggtggcag tgggcaccta tgacagcacc gccgatctgc ttgcggcagg
     1861 cttcggccgg cagcgcagag ccgtaatcgt tctggggtcc accatggtgc tcggcgccct
     1921 caccgcagcc ccgctgcgcg acccgcggct gcggtccatg ccacacctcg ggccgggatg
     1981 gttctcgggc ggttggacaa attttgccgg atcaagcctg gcccttgcgg atgctctcat
     2041 cgcaaagggc aatggcaagg atgatggcgc tgtccctctc gtctggccat atttcgctgg
     2101 cgaacgtgcg cccgtctggg ccccgcatgc cagcggcatg atcgccggcc tgagggccgg
     2161 tacgacagcg tctgcagtcc gccgcggctt cgtgcagggc gtggcccttt ctgccgccga
     2221 tatcgcctac aggctcgccg tcgagaccgg cccgatccgg cgctggaccg tcatcggcgg
     2281 cggcagccgc gacgaagctc tgatgggcga gctggccgat gcgctcgggg cgactctcga
     2341 tatcgtcaag ggggctgcgg atcatctcgg gcctgccgtt cttgccgccc gcagcgcggg
     2401 cgtcgagctg cggctgccga ttgggcgccg ctatcgcccg cgccgtgctg cccatcacgc
     2461 ctatggcgaa agattgagaa tctaccggca ggtctttgcg gcggtgcagc cgcttctgtc
     2521 tggaatccgc catgtcacag gatcggggag cagggtttga tgacagacac gatgagatcg
     2581 gcgcggcttg tcggtatccg gcaaatgcag atcgaccgcc tgccggtgac cccgccgcag
     2641 gcgggcgaga tccaggttcg catcgaggcc tgcggcgtct gtgcgacgga ctcgcggaaa
     2701 tacgagatcg gggtgaatga cggacactac cctttcaatc ccgggcatga atggctcggc
     2761 gaagtctcgg ccatcggaag tggggtgaag gggttccgtg tcggcgatcg cgtctatggc
     2821 gacacctatg gcggctacgc cgatttcgcc accattcccg tcgaccccat cgactggtcg
     2881 cgcgggccgc tgctgctgcc gcgtgacctc gaccgccgcc gcgccatctt catcgaaccc
     2941 ctggccgact gcatccatgc ggtgcgcgac caggcgcggc tggagcctgg ccagaagctg
     3001 gtggtgatcg cggccggcgt catggggctg aagatcattg ccgatgccgc ccggctaggc
     3061 tgcgaggtga tggcggtgga accgctggta ccgcgccagg ccgcagcccg gacattcggt
     3121 gcaagtctcg ttaccgggcc tgagaactgg gccgaagccg cggccgactg gaccggcggg
     3181 cggggcgtcg acgccgtgat cctcaccatc ggaaatcccg atctggtcat gccctgcgtg
     3241 agggctgcgc ggccgggcgg ccgggtggtc ctgttcgctg ggttcggcaa ccgcaaggaa
     3301 gcagtgctcg acctcaacga cattcactac cgcgagattg cgctcatcgg cagcgagtgg
     3361 atcggcacgc cgcccaatca gcgccggcac cgttatgacg aggcgctgga tttccttctg
     3421 gacgcccgcg ttcctttcga ggaccttgtg acctcgacct gcggctttgc aacgctcgaa
     3481 caggcgctcg tcgcccgtca gggcttcacg gggttcaaga tcatgttcga gccggagaga
     3541 gagtcatgat gacgccagcc tgcatcggcg ctgacggacg gtcggtgatc atcgccatcg
     3601 accacgcgct ctactcgtgg ccgtgccgcg gactcgaaga ccggaagtcg atcatatcgg
     3661 cagcgaatgc ggccggcgcc gacgcgatta tcgcgagtta tggcaccatc cgcgacttcc
     3721 ggcgggactt cggccgcgtg aagccgattc tcaagctcga tctgacgtcc gtcacgctgg
     3781 gcgggcacta tccgctgacg gactatgtca tggcctatgg cctcgacgac gccgaacgtc
     3841 ttggtgtcag tacggtcctc aacttcatcc agctgggcgc acccttcgag ctcgaggcgc
     3901 tgcgccagtc cggcaggctt gcggccgaat gcgacaggcg cggcttcacc tatctctgcg
     3961 agatcatgcc ggtcgagagc gccatgtttc cagatccgga agcgccggag gccatggtgg
     4021 cggcctgccg gaccgggcag gaactgggcg cacatgtcat caagaccacc atgccggtcc
     4081 cttcggcgga aatcgctgcg gcggttgcgg cctgcaacat tccggtggtg ctcgccggcg
     4141 gggcccttgc gaaggatgcg gcggcctatg atgccgcgat ctccgccgcg ctgtccgccg
     4201 gcgtggcggg tgtcgccatc gggcgcaatg cctggggcgc agccgatccg gccgcggcca
     4261 tgcagcgctt ttgccggctg attcacggcg ggacggcgaa ggaggtaaag cgggcatgac
     4321 cgttgcgcgc gatctcgatg atcggctcgc catcgtcacc ggcgccagca gcggcatcgg
     4381 ccgaagcgtg gccatgctgc tggcgcggcg cggtgcccgg gtcgttgccg tcggacgcaa
     4441 ccgcgccagg cttgacgaac tcgctgccct ccatgatgga atcgtgccgc atatcgccga
     4501 tctcgcagat cctggttcga ccgaccttgt cttccatgcg gcaacgcgcc tcggcgcggc
     4561 aaccatcctg gtctgcgctg ccggccttcc gggctttacc gacagaccga ttgcgtctca
     4621 gagcttcgag gcctggcgcg ccaccatggc agtgaacctg gattcgccct tcctcatgac
     4681 gcgggccatc gccccgctca tcgccgccgc aagatgggga cggatc
//
LOCUS       DBNGVR010000105         1606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3966965, whole genome shotgun sequence.
ACCESSION   DBNGVR010000105 DBNGVR010000000
VERSION     DBNGVR010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3966965"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..290)
                     /locus_tag="AC16A0_02360"
     CDS             complement(42..290)
                     /locus_tag="AC16A0_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HIR3104202.1"
                     /translation="MNVKQPDQALQRAGPLAALPQLVAELGADPAGVFLGSGLAPSDL
                     RADHFIPLSLFAQLLDRAASVTGRPDFALLLGLRQSRD"
     gene            370..>1606
                     /locus_tag="AC16A0_02365"
     CDS             370..>1606
                     /locus_tag="AC16A0_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3104203.1"
                     /translation="MSVTKFVFIIIALLAARLPVAEAQQVTGELGKPGATTTIDGAQL
                     PAPTPPFGGVIKDDALTSTPWWAPRIVPPKGAPNILLIMTDDSGYGVPSTFGGVIPTP
                     ALDRVAASGLRYTQMHSTALCSPTRAALITGRNHHSAGFGVISEQSTGYPGYNSIIDR
                     NKATIGRILKDNGYATSWFGKDHNVPAFQASQAGPFDQWPIGQGFEYFYGFVGGDANQ
                     WQPNLFRNTTQIYPFEGKPGWNLITGMADDAIDYLNRVNQIQPDKPFFLYYVPGGVHA
                     PHHPTKEWVDKIQAMHLFDDGWEKLRETIFANQKKLGVVPQNAQMGPWPSDILKPWAQ
                     TTDAEKKLFIKQAEVFAAYVAYTDDSIGRVIQAVEDMGKLDNSLIIYISGDNGTSAEG
                     TLVGTPNQMTAYNAILDVPI"
BASE COUNT          344 a          521 c          472 g          269 t
ORIGIN      
        1 agagtgggcg caaagctcat gatcaggccg acagggccga gttagtctcg agactgccga
       61 aggccgagca gcagcgcgaa gtccggccgc ccggttacgc tggctgcgcg gtcaagcagc
      121 tgggcgaaga gcgacagggg aatgaagtga tcggcacgaa ggtccgaggg cgcaagcccg
      181 cttcccaaga agacgccagc ggggtccgcg cccaattcgg ccacaagttg aggcaaggcg
      241 gcaagcgggc cggcgcgctg cagggcctga tctggttgtt tcacgttcat ggcaatggtc
      301 gcagaaaagc taacacgagg tcacaatcga ttgtaaagtt aatcttcggt gcttgcgcga
      361 gaggttctca tgtccgtcac gaagtttgtc ttcataatca ttgcgttgct tgcagcacgg
      421 ctgccagtgg ccgaggccca acaggtgacc ggcgaacttg gcaagccagg cgccaccaca
      481 accattgatg gcgcgcagct gccggcaccg accccgccat tcggcggcgt gatcaaggac
      541 gacgcgctca catccacgcc gtggtgggca ccgcgcatcg tgccgcccaa gggggcgccc
      601 aacatcctgc tgatcatgac cgatgattcc ggctatggcg ttcccagcac cttcggcggc
      661 gtcattccga cgccggcact cgatcgcgtc gccgccagcg gcctccgcta cacccagatg
      721 cattcgactg cactctgctc gcccacccgt gcggccctca tcacgggccg caaccatcac
      781 tcggcgggtt tcggcgtcat ctccgagcag tccaccggct atccgggcta caacagcatc
      841 atcgatcgca acaaggcgac gatcggccgg attctcaagg acaatggcta tgccacctca
      901 tggttcggca aggatcacaa cgtaccggcc tttcaagcca gtcaggccgg acccttcgac
      961 cagtggccca tcggacaggg attcgagtat ttctacggct tcgtcggtgg cgatgccaac
     1021 cagtggcaac ccaacctgtt ccgcaacacg acgcagatct atcccttcga aggaaagcct
     1081 ggctggaacc tgatcaccgg catggctgat gacgccatcg actacctgaa ccgcgtcaat
     1141 cagattcagc ccgacaagcc attcttcctc tactacgttc cgggcggcgt gcatgcgccg
     1201 caccacccga ccaaggaatg ggtcgacaag atccaggcga tgcatctctt cgacgacggc
     1261 tgggagaagc tgcgcgagac catcttcgcc aaccagaaga agctaggcgt cgttccgcag
     1321 aatgcccaga tgggtccgtg gcccagcgac atcctgaagc cctgggccca gaccaccgac
     1381 gcggagaaga agcttttcat caagcaggcc gaggtcttcg cggcctatgt tgcctatacc
     1441 gacgattcga tcggccgggt catccaggcc gtcgaggaca tgggcaagct ggacaactcg
     1501 ctcatcatct acatcagtgg agacaacggg accagcgcgg aaggcaccct ggtcggcacg
     1561 cccaaccaga tgacggccta caacgcgatc ctggacgtcc cgatcg
//
LOCUS       DBNGVR010000106         2581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4162520, whole genome shotgun sequence.
ACCESSION   DBNGVR010000106 DBNGVR010000000
VERSION     DBNGVR010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4162520"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="AC16A0_02370"
     CDS             complement(<1..793)
                     /locus_tag="AC16A0_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3104204.1"
                     /translation="MKRIIVSALALCATAGLAAAQEMKTELGAGEGQVDIVAWPGYIE
                     RGQTDKNFDWVTDFEAKTQCKVNIKTAGTSDEMVALMNEGGFDLVTASGDASLRLISG
                     KRVQPINTALIPSWSTVDERLQNAPWHTVGGVHYGVPYQWGANVLAYNTNVFKEAPTS
                     WAVVFEEQNLPDGKSNKGRVQAFDGPIYIADAALYLMSKKPELGIKDPYELTEEQYKA
                     ALDLLRAQRKIVSRYWHDAFIQIDDFKNEGVVASSSWPFQVNLLRG"
     gene            966..2261
                     /locus_tag="AC16A0_02375"
     CDS             966..2261
                     /locus_tag="AC16A0_02375"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR3104205.1"
                     /translation="MAEEASGGLVVRGGRIIELVARGRNPTMAVDAIFDASRHVVLPG
                     LVNTHHHFYQTLTRAHPKAINKELFDWLKALYPVWARLTPESLRLATRLALAELLMSG
                     VTTTSDHHYVFPKGLDDAMDIQVDEARRLGMRMTVTRGSMNLSVKDGGLPPDSVVQDE
                     DEILADCERVIGKYHQSGDGAMIQVALAPCSPFSVTRSLMCACGELAARHDCRLHTHL
                     AETHDEDEFCKARFGISPLDYLDSVGWLNGRTWLAHGIHFSDAEIARLGRHAVGVCHC
                     PTSNMVLASGQCRTRDLEAAGSPVGLGVDGSASNDSSNLMEGVRHALMINRLTYDATV
                     THLDALRWATRGSARCLGRSDIGEIAVGRQADLAFYTLDELRFSGAGDPLAALVLCGA
                     HRADRVMIAGEWRLVDGVPLGLDVAELRVAHGAAAAQFL"
     gene            2280..>2581
                     /locus_tag="AC16A0_02380"
     CDS             2280..>2581
                     /locus_tag="AC16A0_02380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3104206.1"
                     /translation="MSLAAQYSNRELVPDHPQIIAGWHSDAQAFRARAKADADQSYGA
                     GPRNRFDLFHPRADKGGPLVVFIHGGYWRSFDKSVFSHMAAGATAHGLTVAVPSYSL"
BASE COUNT          430 a          799 c          839 g          513 t
ORIGIN      
        1 cgccgcgcag caggttcacc tggaacggcc acgaggagga ggcgacgacg ccttcattct
       61 tgaaatcgtc gatctggatg aacgcgtcgt gccagtagcg cgagacgatc ttgcgctggg
      121 cgcgcagcag gtcgagcgcg gccttgtact gctcctccgt cagctcgtag gggtccttga
      181 tgccgagctc cggcttcttg gacatcagat agagcgctgc gtcggcaatg tagatcggtc
      241 cgtcgaaggc ctgtacccgg cccttgttgg acttgccgtc cggaaggttc tgctcctcga
      301 agaccaccgc ccagctggtc ggcgcttcct tgaagacatt ggtgttgtag gccagcacgt
      361 tggcgcccca ctggtagggc acgccataat ggacgccgcc caccgtatgc cagggggcgt
      421 tctggaggcg ctcgtccaca gtcgaccaag acgggatgag ggccgtgttg atcggttgca
      481 cgcgcttgcc cgagatgagg cggagcgagg cgtcgcccga ggcggttacg aggtcgaaac
      541 cgccttcgtt catgagggcg accatttcgt ccgatgtgcc ggccgtcttg atgttgacct
      601 tgcactgggt ctttgcctcg aaatccgtga cccagtcgaa gttcttgtcg gtctggccac
      661 gctcgatata gcccggccag gcgacgatat cgacctggcc ttcgccggcg cccagctcgg
      721 ttttcatctc ttgcgccgcg gccaggccgg cggtcgcgca gagcgccaaa gctgaaacga
      781 tgatgcgttt catggatgtc atgattgtct tactccctgc gggccgtttg tcgggatgcc
      841 agcttggcac cttgttgaac ccgtgcgact attgtgaacc atcttgtggg accaattcaa
      901 cccccggtcc cggcacggag ctgcacaacg tggaaagtct cttcatccga tcaccgctcg
      961 catgcatggc cgaggaggcg tccggcgggc tagtcgtaag ggggggcagg atcattgaac
     1021 tggtggcgcg aggcaggaac ccaaccatgg cggttgacgc catcttcgat gcatcgcggc
     1081 atgtggtgtt accggggctg gtgaacacgc accatcactt ctatcagacc ctgacccggg
     1141 cccatcccaa ggccatcaac aaggaactct tcgactggct caaggcgctc tatccggtgt
     1201 gggcacggct cacgccagag tccttgcggc tggccacgcg gctggcgctg gcggaactgc
     1261 tgatgtccgg cgtgacgacc acttccgatc accactatgt gtttccgaag ggtctcgacg
     1321 atgccatgga tatccaggtg gacgaggcca ggcgcctcgg catgcgcatg acggtgacgc
     1381 gcgggtcgat gaatctttcg gtgaaggacg gtgggctgcc gccggacagc gtggtgcagg
     1441 acgaggatga gatcctcgcc gattgcgaac gtgtcatcgg caagtatcac cagtccggcg
     1501 atggcgccat gatccaggtg gctctggcac catgttcgcc cttctcggtc acccggtcgc
     1561 tgatgtgcgc ctgcggcgag ctggcggccc gccacgactg ccggctccac acacaccttg
     1621 cggaaaccca cgacgaggac gaattctgca aggcccgctt cggcatctcg ccgctggatt
     1681 acctcgactc cgtgggatgg ctcaatggcc ggacctggct ggcccatggc atccattttt
     1741 ctgatgcgga gatcgcccgg ctgggccggc acgcggttgg agtgtgccac tgtccgacct
     1801 ccaacatggt gctggcctcc gggcaatgtc gcacacggga cctcgaggcg gcgggctcgc
     1861 cggttggact gggcgtcgat ggatcagcct ccaatgacag ctccaacctc atggagggtg
     1921 tccgccacgc gctcatgatc aatcggctga cttatgatgc gaccgtgacc cacctggatg
     1981 cgctccgctg ggcaacaagg ggctcggcgc gctgcctggg ccggtccgac attggcgaga
     2041 tcgccgttgg acgtcaggcc gatctcgcgt tctacacgct cgatgaattg cgtttctccg
     2101 gtgcgggcga tcctctggcg gcgctggtcc tgtgcggtgc ccacagggca gaccgggtga
     2161 tgatcgctgg cgaatggcgt ctggtcgacg gggttccgct gggccttgat gtcgcggaac
     2221 tgcgcgtcgc tcatggtgcc gcagcggcac agttcctcta accattccgg agttgatcga
     2281 tgagcctcgc cgcacagtac agcaaccggg agttggttcc ggaccacccg cagatcatcg
     2341 cgggctggca cagcgatgcc caggcctttc gggcgcgcgc caaggcggat gccgaccagt
     2401 cctatggcgc aggtccgcgc aatcgatttg acctgtttca tcccagggca gacaaaggtg
     2461 gtcctttggt ggtcttcatc catggcggct actggcggtc cttcgacaag tcggtcttca
     2521 gccacatggc ggccggagcc accgcgcatg gcctaacggt ggcggtgccg agctacagcc
     2581 t
//
LOCUS       DBNGVR010000107         2266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2396149, whole genome shotgun sequence.
ACCESSION   DBNGVR010000107 DBNGVR010000000
VERSION     DBNGVR010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2396149"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2266)
                     /locus_tag="AC16A0_02385"
     CDS             complement(<1..>2266)
                     /locus_tag="AC16A0_02385"
                     /inference="COORDINATES: protein motif:HMM:NF017930.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HIR3104207.1"
                     /translation="TERLKSDVHTGVLTDIAKQCRFMAYGMEIGFILDRQRHLLSIGY
                     RMEDETLDQSCYDLLASEASLTSFLAIAKGDVAEGHWLRLGRPVTAVNSAACLLSWSG
                     SMFEYLMPLLVLQNRNGTLVNQTHRLAVERQISYTKRRGTPWGISESAYAVRDLGFTY
                     QYSNFGVPGLGLKRGLADSHVIAPYATALAAMIAPAAAAENYRHLQSIGGDGRFGFYE
                     AVDFTPSRLRTGHSHEVIRAFFAHHQGMTITAIHNAVSGGKLRSHFHRENIVRAAELM
                     LQERAPSHLPARIIGTGRPGEMVAVTSDTRELSRHILPQQSGPVSHLLSNGRYAVMLT
                     GRATGYSRWKGIAINRWRRDHIADESGLAIYLRDLETGLVWPAAPSPSARGGVEGTAT
                     FGQEKAEFHRTDVGITTHLECVISTEDDSEARCLQLVNASDTTRRVEYTSFMELALAL
                     PEADDAHPAFSKLFVETEFLPELQALIAHRRKRLDSDPEIWVAQFIVSDNKSATAVQY
                     ETARQTFLARGRTVTQAMTASSGEQFTSATGTVIDPVFAMRQTVRLKPHRRSRSVIWT
                     IVADSRDALLEKVSRHRTMAVFERVQVLAWTQSRILLRHLSIDAGEANLFQILATSLV
                     YSMPRFRPVPAAIARDMQPQATLWPLAISGNRPIVAVTIREREDMGLVRQILRAQDYW
                     QEKGLAADVIIINDRRTSYLQELQKDLEDLVGKERVSRSRQGSQEVGEIFALRADLLQ
                     PGAEEAIMSAAHVVL"
BASE COUNT          439 a          700 c          687 g          440 t
ORIGIN      
        1 aagcacgaca tgcgcggccg acatgatcgc ttcctcggcc ccaggttgga gcaggtcagc
       61 acggagtgcg aaaatctcgc ccacctcctg agacccctgc cgcgacctgc tgaccctctc
      121 cttgcccacc aggtcctcaa gatccttctg cagctcctgc agataggacg tgcgccggtc
      181 atttatgatg atcacatcag cggcaagacc cttctcctgc caataatcct gggcgcgcag
      241 gatctgcctg accaggccca tatcctcacg ttccctgatg gtcacggcga cgattggtct
      301 gtttccggaa atcgccagtg gccagagtgt cgcctgtggc tgcatgtcac gggcaatcgc
      361 cgccggtacg gggcgaaagc gcggcattga atagaccagg gatgtggcca ggatctggaa
      421 caggtttgcc tcgcccgcat caatcgaaag atgccgcaaa agaatgcggg actgcgtcca
      481 ggccagaacc tgcacgcgct cgaacacggc catggtgcga tgccggctga ccttctccag
      541 cagcgcgtct cggctgtcgg cgacgatcgt ccagatgacg cttcgcgacc tccggtgtgg
      601 cttgagccgt acagtctgcc gcatggcgaa gaccggatcg ataaccgtcc cggtcgccga
      661 cgtgaactgc tcgccggatg acgccgtcat ggcctgggtc actgtgcgtc cgcgtgcaag
      721 aaacgtctgg cgcgccgtct catactgaac cgccgtcgcc gacttgttgt cgctcacgat
      781 gaactgcgca acccagatct cgggatcgct gtccaggcgc ttgcggcggt gggcaatcag
      841 agcctgaagc tccggaagga actccgtttc cacgaagagc tttgagaagg ccggatgagc
      901 gtcatcggcc tccggcaggg cgagggccag ctccatgaaa ctggtgtatt cgaccctccg
      961 ggtggtgtcc gacgcattga ccagttgaag acatcgggcc tcagagtcgt cctccgtgga
     1021 aatgacgcat tccaggtgag tcgtgatccc cacatcggtc cggtgaaatt ccgccttttc
     1081 ctggccgaag gtggcggttc cttcgacccc ccctctcgcg cttggtgatg gcgccgcggg
     1141 ccagaccaaa cccgtttcga gatcgcggag gtagatggcc agaccggatt cgtcggcgat
     1201 gtgatcgcgg cgccagcgat tgatggcaat gcccttccac cggctatagc ccgtcgcccg
     1261 gcccgtcagc atgaccgcgt agcgtccgtt ggacagtaga tggctcacag gaccggactg
     1321 ctgtggaaga atatgccggg acagttccct cgtgtcagat gtcacagcta ccatctcacc
     1381 aggccgcccg gttccaatga tccgggccgg cagatggcta ggtgcgcgtt cctgaagcat
     1441 gagttccgcg gcacggacga tgttctcgcg atgaaaatgc gagcgaagct tgccacctga
     1501 gacggcattg tggatcgcgg tgatggtcat gccctgatga tgcgcaaaga aggcccggat
     1561 gacttcatgg gagtggcctg tccgcagcct cgatggcgtg aagtcgaccg cttcgtagaa
     1621 gccaaatctc ccatcgccgc cgatgctctg aaggtggcga tagttttccg cggccgccgc
     1681 tggggcaatc atcgccgcca gcgccgttgc gtagggtgcg atgacgtgac tgtcggccag
     1741 ccctcgcttc aatccaagcc ccggcacgcc gaaattcgag tactgatagg tgaaacccag
     1801 atcgcggacc gcataggctg actccgagat gccccatggt gtaccccggc gcttcgtata
     1861 ggagatctgc ctctcgacgg cgaggcgatg agtctggttc acgagcgttc cgttacggtt
     1921 ctgcaggacc agcagtggca tcaggtactc gaacatcgag cccgaccacg acaacagaca
     1981 cgcggcactg ttgacggcgg ttacgggccg acccaggcgc agccagtgac cttctgcgac
     2041 atctcccttt gcgatggcga gaaagctggt gagcgatgcc tcggatgcca gaagatcgta
     2101 gcagctctgg tcgagcgtct cgtcctccat gcggtagccg atcgacagaa gatggcgctg
     2161 tcgatcgaga ataaagccga tttccatgcc ataggccatg aatcggcatt gcttggcaat
     2221 gtcggtcaac acgccggtat ggacatccga tttcagtctc tccgtc
//
LOCUS       DBNGVR010000108         2652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5342687, whole genome shotgun sequence.
ACCESSION   DBNGVR010000108 DBNGVR010000000
VERSION     DBNGVR010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5342687"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="AC16A0_02390"
     CDS             <1..248
                     /locus_tag="AC16A0_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HIR3104208.1"
                     /translation="KLPTWSIKEARGALERLVGAMDDWAPFDSWLLDYLADPGMRRSV
                     MASSFTASLELAREGVLDLRQDGPFRPIFMRRHAPAA"
     gene            324..1055
                     /gene="scpB"
                     /locus_tag="AC16A0_02395"
     CDS             324..1055
                     /gene="scpB"
                     /locus_tag="AC16A0_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3104209.1"
                     /translation="MDETDETAEAGEGAVVTLHPRADRNQHLRIIEALLFAAAQPVDQ
                     AKLAGFLPEGTDLNGLLADLKANYANRGVNLVEVAGKWSFRTAEDLSFLLRRESVEQR
                     KLSKAAMETLAIIAYHQPVTRAEIEDIRGVAISKGTLDMLLEIGWAKMRGRRKTPGRP
                     VTYGTTEAFLSHFGLNEVSDLPGLQELKGAGLLDANLPPGFDMPIPRGSDELTAEEDP
                     LDGTEMEQPLEMHLPEPGESDPKNG"
     gene            complement(1059..1874)
                     /locus_tag="AC16A0_02400"
     CDS             complement(1059..1874)
                     /locus_tag="AC16A0_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104210.1"
                     /translation="MRSESSSRAPRALSLAAIAGLLFLHVPLLIIFLYAFTTEEKSYK
                     FPPPGFTLKWFSVVWERQDIWDAIWLSVKVAGIATAIALVLGTCAAAAVSRSRFFGRD
                     YISLLFILPIALPGIITGISLRSAFSMMDIPFSTWTIVLGHATFCIVVVYNNALARFR
                     RTSGSMIEASMDLGADGFQTFRYVVLPQIGSALLAGGMLAFALSFDEVIVTTFTAGQQ
                     ATLPIWMLNELIRPRQRPVTNVVAIFVIAVTFVPILAAYYLTRDGEHTGGAGK"
     gene            complement(1864..>2652)
                     /locus_tag="AC16A0_02405"
     CDS             complement(1864..>2652)
                     /locus_tag="AC16A0_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003566682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104211.1"
                     /translation="LLHSFYSIDEFSGFIVYEFTLSTYAELFRLSNLDVILRSVVMAI
                     AVTIACAIMAFPISYYAARYARGKWKAAFYLLVMLPLWSSYLVKVYAWKLILAKEGII
                     SWLAEQTHLTWLLDGILAIPVIGGPSLSISYLGTFIVFCYVWLPYMILPTQAALERVP
                     GNLIEASADLGAEPRQTFRTVIFPLALPGIIAGSIFTFSLTLGDYIIPQIVGTSTYFL
                     GQAVYTLQGTAGNIPLAAAFTVVPIVIMLIYLAIAKRMGAFDAL"
BASE COUNT          561 a          764 c          914 g          413 t
ORIGIN      
        1 gaaagctgcc gacatggtcg atcaaggaag cccgcggcgc gctggagcgc ctggttggcg
       61 ccatggatga ctgggcgccc ttcgacagct ggttgttgga ctatctggcc gatccaggca
      121 tgcgtcgctc ggtgatggca tcgagcttca ccgcctcgct ggaactggcc cgcgagggcg
      181 ttctcgatct tcggcaggac gggcccttcc gtccgatctt catgcgccgg catgcgccgg
      241 cggcttgacc tttccgtctt ggagcaatca gtatgcagac gaccaatgcc agcctagggg
      301 aactcccgcc acaggagccc gagatggacg agacggatga aacggccgaa gcgggcgaag
      361 gcgctgtggt gacacttcat ccgcgtgccg accgcaacca gcatctgcgc atcatcgaag
      421 cgctgctctt tgcggcggct cagcccgtgg accaggccaa gctggcgggc ttcctgcccg
      481 aaggcaccga cctcaacggg ctgcttgccg atctcaaggc caactacgcc aatcgcggtg
      541 tcaatctcgt tgaagtggca ggcaagtggt cgttccggac agcggaagac ctgagtttcc
      601 tgctgaggcg ggagtcggtg gagcagcgca agctctccaa ggccgcgatg gagaccctgg
      661 cgatcatcgc ctatcaccag ccggtgacgc gcgccgagat cgaggatatc cgcggtgtcg
      721 cgatctcgaa gggaaccctg gacatgcttc tggagatcgg ctgggcgaag atgcgtggcc
      781 gccgcaagac tcccggccgg ccggtgacct atggcaccac cgaggctttc ctcagtcact
      841 tcgggctcaa cgaggtgagc gatcttccgg gcctgcagga actgaagggc gcagggctgc
      901 tcgatgccaa tctgccgccg ggtttcgaca tgccgatccc gcgcggcagc gacgaactca
      961 ccgccgagga agaccctctc gacggcacgg aaatggagca gccgctcgaa atgcatctgc
     1021 cggagcctgg cgagtccgat cccaagaatg gctgaagcct atttgccggc cccgcctgtg
     1081 tgttcgccgt cgcgcgtcag atagtaggcg gcgagaatgg gaacgaaggt cacggcgatc
     1141 acgaagatgg ccaccacatt ggtgaccggc cgctgacgcg ggcggatcaa ttcgttcagc
     1201 atccagatcg gcagcgtcgc ctgctggccc gccgtgaagg tggtgacgat cacctcgtcg
     1261 aaggagagcg caaaggccag catgccgccc gccagcaagg ccgagccgat ctggggcaac
     1321 accacatagc ggaacgtctg gaagccatcg gcaccgaggt ccatggaggc ctcgatcatg
     1381 ctgccggagg tgcggcggaa gcgcgccaga gcgttgttgt acacgacgac gatgcagaag
     1441 gtggcatggc ccagcacaat ggtccaggtc gagaagggga tgtccatcat gctgaaggcg
     1501 gagcgcagcg agatgccggt gatgatgccg ggcagggcaa tcggcagaat gaagagcagc
     1561 gagatatagt cgcgcccgaa gaagcgggag cgtgagacgg cggctgcggc acaggtgccg
     1621 agaacgagcg cgatcgccgt ggcgatgccc gccaccttca ccgagagcca gatggcgtcc
     1681 cagatgtcct ggcgctccca gaccacggag aaccacttca gcgtgaagcc cggcggtgga
     1741 aacttgtagc ttttttcctc tgtcgtgaaa gcgtagagga agatgatcag cagcggcaca
     1801 tgcaggaaga gcagtcctgc gatcgctgcg aggctgagcg cgcgcggcgc cctggaagaa
     1861 gactcagagc gcatcgaagg cccccatgcg cttggcgatc gcgagataga tcagcatgat
     1921 gacgatggga accacggtga aggccgcggc caaagggatg ttcccggcgg ttccctgcag
     1981 ggtgtagacg gcctggccca ggaagtaggt ggaagtcccg acgatctgcg ggatgatgta
     2041 gtcgccgagc gtcaacgaga aggtgaagat cgagccggcg atgatgccgg gaagcgccag
     2101 cgggaagatc accgtccgga aagtctggcg cggctcggcg ccgagatcgg cggacgcctc
     2161 gatcaggttg cccggaacgc gttcgagcgc cgcctgggtt ggaaggatca tgtagggcag
     2221 ccagacgtag cagaagacga tgaaggtgcc gaggtagctg atcgacaggg acggcccacc
     2281 gatgaccgga atcgccagga tgccgtcgag cagccaggtg agatgggtct gctctgccag
     2341 ccagctgatg atgccctcct tggcgaggat cagcttccag gcatagacct tgaccaggta
     2401 gctggaccac agaggcagca tgacgagaag atagaatgcg gccttccact tcccgcgggc
     2461 atagcgcgcg gcatagtagg agatggggaa ggccatgatg gcgcaggcga tggtgacggc
     2521 gatggccatc accacagacc ggaggatgac gtcgaggttg gacagccgaa agagttcggc
     2581 ataggtcgac agcgtgaact cgtagacgat gaagccggaa aactcgtcga tcgagtagaa
     2641 gctgtgcagc aa
//
LOCUS       DBNGVR010000109         3599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1609562, whole genome shotgun sequence.
ACCESSION   DBNGVR010000109 DBNGVR010000000
VERSION     DBNGVR010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1609562"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC16A0_02410"
     CDS             <1..356
                     /locus_tag="AC16A0_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR3104212.1"
                     /translation="FNGIGGPEYVYSLLTGYEEPPAELAAEAPEGKSYNPYFAAGHWI
                     GMPAPLTDDQVTYDDGTPATVDQMAKDVAAFMAWSAEPKMEERKRLGFMVMIYLAVLA
                     LLLYLVKKRLWAKEH"
     gene            452..1324
                     /locus_tag="AC16A0_02415"
     CDS             452..1324
                     /locus_tag="AC16A0_02415"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HIR3104213.1"
                     /translation="MVKTVLGIIGGSGVYDIEMDDARWEAARSPWGDPSDQLRHGEIG
                     GLPVVFLPRHGRGHVYSPSTINYRANIDAMKRAGVTDLVSLSAVGSLKEELPPGTFVL
                     VDQFIDRTFAREKSFFGTGCVGHVPFSHPVSPLLQAAVARALAAEKLRHAIGGTYVVM
                     EGPQFSTLAESRLYRSWDAHVIGMTNMPEAKLAREAEICYCTVAMVTDYDSWHEEHGT
                     VDVAKVIAVMQSNSGNARRLVRRIAQELPQDHQPCPIGSDRALEHAIMTAPAKRDPAV
                     LAKLDAVAGRVLKR"
     gene            complement(1325..1939)
                     /locus_tag="AC16A0_02420"
     CDS             complement(1325..1939)
                     /locus_tag="AC16A0_02420"
                     /inference="COORDINATES: protein motif:HMM:NF017816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HIR3104214.1"
                     /translation="MMKPGILVAAAVLVLFPDFAEAKPAHSTKYTYYTVGGNSAESIY
                     KAMLNKGPRVNGAKAYAATTATTRQDGKLMQSKSCRIEDYQLRLDFVIKLPKIKNEKA
                     LASADRERWKQFSSFLKKHEETHRSIWLECAAEMERKVRAIKAKSCDEADAKAEKLWE
                     QTRAACSKKHDAFDLAEQKKLMKHPFVQLVYKRSSSNTRAAAAQ"
     gene            2220..2432
                     /locus_tag="AC16A0_02425"
     CDS             2220..2432
                     /locus_tag="AC16A0_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3104215.1"
                     /translation="MRQNGTVKFFNQAKGYGFISPEGGGKDVFVHITAVQRSGIPELN
                     EGQKISFEVQDDKKGRGLQAVELQLI"
     gene            2677..3375
                     /locus_tag="AC16A0_02430"
     CDS             2677..3375
                     /locus_tag="AC16A0_02430"
                     /EC_number="4.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012659.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0033897 - ribonuclease T2 activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2 family protein"
                     /protein_id="HIR3104216.1"
                     /translation="MPGSLLARHLRRLAVPSAAAITVLAALLILPALARDSRPGQFDY
                     FVLSLSWSPTFCAGPDGDRDPSQCGPGRRFAFVVHGLWPQNRDGTWPEFCASRETWVP
                     QEVIDGMMRIMPSKRLIIHQWRKHGSCSGLTIENYFLATRLLFDKLKIPARYLSPQAK
                     IQTTPEQLVLDFVKTNRDLTADMISVQCGNARDTARLSELRICIDRRGNFGQCGAGAQ
                     RQCRAQSLVMLPVR"
     gene            3383..>3599
                     /locus_tag="AC16A0_02435"
     CDS             3383..>3599
                     /locus_tag="AC16A0_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HIR3104217.1"
                     /translation="MKRALVFQHMNHYHPGRFLDYFAEDGIIPDHVRIFEGQDIPDLA
                     PYDFLFVLGGSQDTWQEEQYPWLRAEKE"
BASE COUNT          653 a         1065 c         1147 g          734 t
ORIGIN      
        1 tgttcaatgg catcggcgga ccggaatatg tctattccct gctgaccggc tatgaggagc
       61 ccccggccga acttgccgcc gaggcgccag agggcaagag ctataatccc tacttcgccg
      121 cgggccactg gatcggcatg cccgctccgt tgaccgacga tcaggtgacc tacgacgacg
      181 gcacgcccgc gaccgtcgat cagatggcca aggacgtggc ggccttcatg gcctggtcgg
      241 ccgagcccaa gatggaagag cgcaagaggc ttggcttcat ggtgatgatc tatctggcgg
      301 tgctggccct gctgctctac cttgtcaaga agcgcctgtg ggcgaaggag cactgaacca
      361 gcacattggt tgacaaaggg cccggatctt ccgggccttt tcgtttctgt gggcctcggg
      421 cattgtcgga tgggcacagg agtgggacga catggtcaag acggttctcg gtatcatcgg
      481 cgggtcgggc gtctacgaca tcgagatgga cgatgcccgt tgggaggcgg cgagatcgcc
      541 ctggggtgat ccatccgatc agctgcgcca tggcgagatc ggcgggctgc cggtggtgtt
      601 cctgccgcgc catggtcgcg gtcatgtcta ttcgccgagc accatcaact accgtgcaaa
      661 tatcgacgcg atgaagcggg cgggggtgac ggatctcgtc tctttgtcgg cggtgggttc
      721 gctgaaggag gaattgccgc cggggacatt cgttctggtc gaccaattca tcgaccggac
      781 ctttgcgcgc gagaagagct tcttcggcac tggctgtgtg ggacacgtgc cgttctcgca
      841 tccggtctcg cccctgctgc aggcggcagt cgcgcgggcg ctggcggccg agaagctgag
      901 gcacgccatc gggggaacct atgtcgtcat ggaaggcccg cagttctcca cgctggccga
      961 gtcgaggctc tacaggtcgt gggatgcgca tgtcatcggc atgaccaaca tgccagaggc
     1021 caagctcgcg cgcgaggcgg agatctgcta ctgcacggtc gccatggtga ccgactatga
     1081 ctcctggcac gaagagcacg gtacggtcga tgttgccaag gtgatcgccg tgatgcagtc
     1141 aaattccggc aatgcgcggc gcctcgtgcg gcggatcgcg caagagctgc cgcaggacca
     1201 ccagccatgc cccatcggct cggaccgggc gcttgaacat gccatcatga cggcgccggc
     1261 gaagcgcgat ccggccgtgc tggcgaagct cgatgcggtg gcagggcggg tgctgaagcg
     1321 gtagttactg cgccgcggcg gcgcgcgtgt tgctcgagct gcgcttgtag acaagctgaa
     1381 cgaagggatg cttcatcagc ttcttctgct cggccagatc aaatgcatca tgtttcttcg
     1441 agcaggctgc gcgggtctgc tcccagagct tctcggcctt ggcatcggct tcgtcgcagg
     1501 atttcgcctt gatcgcccgc actttgcgct ccatttcggc agcgcattcg agccagatcg
     1561 aacgatgagt ttcctcgtgc ttcttgagga agctggagaa ttgcttccag cgttcgcggt
     1621 cggcactggc cagtgctttc tcgttcttga tcttgggcag cttgatcacg aaatcgagac
     1681 ggagctgata atcctcgatc cggcaagact tggactgcat cagcttgccg tcctgccgcg
     1741 ttgtcgccgt cgtagcagca taggccttgg cgccattcac gcgcggccct ttgttgagca
     1801 tggccttgta gatggattcg gcagagttcc cgccaacagt atagtaagta tacttcgtgg
     1861 agtgagctgg tttcgcttcg gcgaaatccg gaaacagaac aagcactgct gcggcgacca
     1921 gtatgcccgg cttcatcatg caaacccctc tcgtgccgcc agtattggca tgcttttgcg
     1981 acaaatcaag ttacgactga tgcaggaatc tgccctcttg cccgtgacat ggctctgcct
     2041 catacttagt cagcgttcgg ggctttcccc ggcgcggcgg tgcggctgtt caggtcccgg
     2101 tgaggcggac aggtctcgca gggcccggtg gccacccggt agtttgcttt cgcttggccg
     2161 ggcgtccaaa gtctggtcct gtggatgctt gggataagcg atcaggagac gtgcttccaa
     2221 tgcgccagaa cggcacggtg aagttcttca atcaggccaa gggttacggt ttcatttctc
     2281 cggaaggcgg cggcaaggat gtgttcgtgc acatcacggc cgtgcagcga tccgggattc
     2341 cagagctgaa cgaaggccag aagatcagct tcgaagtcca ggacgacaag aaggggcggg
     2401 gcctccaggc ggtcgaacta cagctgattt agcctgcttc gcctcctgtc cgtcatgtgt
     2461 taactaggga catggggtaa gctggtacag cacgctgaga ttggccctga gtatgggggt
     2521 gctcgtctat ggcctcgacg gatcggcggc acataactgc acttgcaggg ccggcggcac
     2581 cgactatgcg caaggcgaag tccggtgcat catgggcaag ctctcccgct gcgacatgaa
     2641 ccagaacaat tcgagttgga aagtcatcgc cgaaacatgc ccggaagcct ccttgcccgc
     2701 cacctccggc gccttgctgt cccgtctgct gccgcgatca ctgtccttgc cgctctgctg
     2761 atcctgcccg ccctcgcgcg ggacagccgg ccaggccagt tcgactactt cgtcctttcg
     2821 ctgtcctggt cgccgacctt ctgtgccgga ccggacgggg accgcgatcc ctcgcaatgt
     2881 gggccgggac ggcgattcgc tttcgttgtc cacgggcttt ggccgcagaa ccgggacggg
     2941 acatggcccg agttctgcgc gtcccgcgag acctgggtgc cgcaggaggt gatcgacggg
     3001 atgatgagaa tcatgccctc caagcggctg atcatccacc agtggaggaa gcacggcagc
     3061 tgctccggtc tgacgatcga gaactatttc ctggccacgc ggcttctctt cgacaagctg
     3121 aagattccgg cacgctatct gtcgccccag gcgaagatcc agacaacgcc cgaacagctg
     3181 gtgctggatt tcgtcaagac caaccgcgac ctgacggcgg acatgatctc cgtgcaatgc
     3241 ggcaatgcga gggataccgc gcggctctcc gagctccgca tctgcatcga caggcgcgga
     3301 aatttcgggc aatgcggggc cggagcccag cggcagtgca gggcacaaag cctggtcatg
     3361 ctgcccgtga gataggattg tcatgaagcg cgcccttgtc ttccagcaca tgaaccacta
     3421 ccatcccggc cggttcctcg actatttcgc agaggatgga atcatcccgg atcacgtgcg
     3481 tatcttcgaa gggcaggaca tcccggatct cgccccctac gactttcttt tcgttcttgg
     3541 gggatcccag gatacgtggc aggaggagca gtatccctgg ctcagggccg agaaggagg
//
LOCUS       DBNGVR010000110         3221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1807737, whole genome shotgun sequence.
ACCESSION   DBNGVR010000110 DBNGVR010000000
VERSION     DBNGVR010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1807737"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16A0_02440"
     CDS             <1..307
                     /locus_tag="AC16A0_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HIR3104218.1"
                     /translation="KWHSDTVSAILIPEGFDSAALVRHAYTRYNLSLGMGLNKVAGKV
                     FRIGHLGDLNEIMCLTALAGSEMAMRDMGIPVEAGSGVAAAIEFYRTQTATPALKAA"
     gene            320..1516
                     /locus_tag="AC16A0_02445"
     CDS             320..1516
                     /locus_tag="AC16A0_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HIR3104219.1"
                     /translation="MDIHEYQAKEILARFGVPVPKGALAYSPEQAAYRAREIGGKLWV
                     VKAQVHTGGRGKAGGVKLCRSEDEVMEAADDLLGKKLVTHQSGPAGKIVYRLYVEGGV
                     AIVKELYLGFVLDRKSERVMVVASSEGGMEIEEIAAEKPQSIFKVSVEPAVGMQAFQA
                     REIAFALGLAPSQIQSAVNAILGCYRAFEELDATMVEINPLIVTEDDHVMALDAKMTF
                     DDNAMFRHPALAELRDKSQEDPRETHANDRGLSYVGLTGKIGCIVNGAGLAMATMDMI
                     KLAGGEPANFLDIGGGATPERVAKAFKLVTMDKNVEAILVNIFAGINRCDWVAEGVVQ
                     ALEKNPVGVPVIVRLAGTHVEEGRKILARSGLPIIRANTLAEAAERAVSAWKNNNTVS
                     LKVVNA"
     gene            1513..2403
                     /gene="sucD"
                     /locus_tag="AC16A0_02450"
     CDS             1513..2403
                     /gene="sucD"
                     /locus_tag="AC16A0_02450"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3104220.1"
                     /translation="MTILLDRSTPVLVQGITGKIGSFHTKEMLEYGTHIVGGVTPGKG
                     GLTEQGVPVFDTMKEAVAKTGATASVVFVPPPFAADSIMEAADAGVKLCVAITDGIPA
                     QDMIRVKRYMRRYRAENRMRLVGPNCAGVISPGKALLGIMPGNIYLQGHIGIVGRSGT
                     LGYEAAAQMKDLGIGVSTSVGIGGDPINGSSFKDILELFEADPETHAVVMIGEIGGPQ
                     EAEAAEFIRDHMTKPVIAYVAGLSAPKGRKMGHAGAIISAFGEGASEKVELLREVGVA
                     IAATPAEIGSTVATRLKKAA"
     gene            2446..>3221
                     /locus_tag="AC16A0_02455"
     CDS             2446..>3221
                     /locus_tag="AC16A0_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3104221.1"
                     /translation="MSFFTVEPAPARLNRSELAVPGSQPQMFEKAAKSAADVVFLDLE
                     DAVAPDDKAQARKNIIQAIGDIDWGSKALSVRINGLDTHYMYRDVVDVLEQASERLDL
                     IMIPKVGTAADVYAVDMLCTQVETARGRRKRVGFEMIIETALGMQNVHEIAAASKRNE
                     SLHFGVADYAASTKARTTSIGGANADYAVLTEPLEDGSRQTHWGDMWHYAISRMVVAA
                     RANGLRPVDGPFGNFSDADAFKAAARRAAVLGCEGKWAIHP"
BASE COUNT          608 a         1082 c         1023 g          508 t
ORIGIN      
        1 gaagtggcat tccgacacgg tgagcgccat cctgattccg gaaggcttcg attccgccgc
       61 tctcgttcgt cacgcctaca cgcgctacaa cctgtcgctc ggcatgggcc tgaacaaggt
      121 cgccggcaag gtcttccgca tcggtcacct cggcgatctc aacgagatca tgtgcctcac
      181 cgcactggcc ggatccgaaa tggccatgcg cgacatgggc atccccgtcg aggctggatc
      241 cggcgtcgcg gccgcgatcg agttctaccg cacgcagaca gcaaccccgg ccctcaaggc
      301 cgcataagga cttctcatca tggatatcca cgaataccag gccaaggaaa tcctggcccg
      361 cttcggtgtg cccgtgccca agggcgcgct cgcctacagc cccgaacagg ccgcctatcg
      421 tgcccgcgag atcggcggca agctctgggt cgtcaaggcc caggtccaca ccggcggccg
      481 cggcaaggct ggcggcgtga agctctgccg cagcgaggac gaggtgatgg aggccgccga
      541 cgacctgctc ggcaagaagc tggtgacgca tcaatccggc ccagccggca agatcgtcta
      601 ccgcctctat gtcgaaggcg gcgtcgctat cgtgaaggag ctctatctgg gcttcgtgct
      661 cgaccgcaag tcggaacgcg tgatggtggt tgcctcatcc gagggcggca tggagatcga
      721 ggagatcgcc gccgagaagc cgcagtcgat cttcaaggtc agcgtcgaac ccgccgtcgg
      781 catgcaggcc ttccaggccc gcgaaatcgc cttcgccctc ggtcttgcgc caagccagat
      841 ccaatcggcc gtcaacgcca tcctcggctg ctaccgcgcc ttcgaggaac tggatgcaac
      901 catggttgag atcaacccgc tgatcgtcac cgaggacgat cacgtcatgg cgctcgatgc
      961 caagatgaca ttcgacgaca acgccatgtt ccgccatccg gcgctggcgg aactccgcga
     1021 caagagccag gaagacccac gcgagactca cgccaacgac cgtggcctgt cctacgtcgg
     1081 cctgaccggc aagatcggct gcatcgtgaa tggtgcaggc ctcgccatgg cgacaatgga
     1141 catgatcaag ctggccgggg gcgaacctgc caacttcctc gacatcggcg gcggcgccac
     1201 gcccgagcgg gtggccaagg ccttcaagct cgtgacgatg gacaagaacg tcgaggcgat
     1261 cctggtgaac atcttcgccg gcatcaaccg ctgcgactgg gtggccgaag gcgtcgtaca
     1321 ggcgctggag aagaacccgg tcggtgtgcc ggtcatcgtg cggctggctg gaacgcatgt
     1381 cgaggaaggg cgcaagatcc tggcacgctc gggcctcccc atcatccgcg ccaacacgct
     1441 tgccgaagcc gccgaacgtg ccgtctcggc gtggaagaac aacaacactg tcagcctgaa
     1501 agtggtgaac gcatgaccat ccttctcgat cgttcgacgc cggtcctggt gcagggcatt
     1561 accggcaaga tcggctcctt ccacaccaag gaaatgctgg agtacggcac ccacatcgtg
     1621 ggcggggtca cacccggcaa gggcggcctg accgagcagg gcgtgcccgt cttcgacacc
     1681 atgaaggaag cggttgccaa gaccggcgca acggcctcgg tcgtcttcgt gccgccgccc
     1741 ttcgccgcag actcgatcat ggaagcggca gacgccggcg tgaagctctg cgtggccatc
     1801 accgacggca tcccggccca ggacatgatc cgggtgaagc gctacatgcg ccgctaccgt
     1861 gccgagaacc gcatgcgtct cgttgggccg aactgcgcgg gcgtcatctc gcccggcaag
     1921 gccctgctcg gaatcatgcc cggcaacatc tacctgcagg gccatatcgg gatcgtcggc
     1981 cgttccggca cgctcggcta cgaagccgca gcacagatga aggatctcgg catcggcgtc
     2041 tcgaccagcg tcggaatcgg cggcgatccg atcaacggct cgtccttcaa ggacatcctc
     2101 gaactcttcg aggccgatcc cgaaacccat gccgtcgtca tgatcggcga gatcggcggt
     2161 ccgcaggagg ccgaggctgc cgaattcatt cgcgaccaca tgaccaagcc ggtgatcgcc
     2221 tatgtcgccg gtctctcggc gcccaagggc cgcaagatgg gccatgccgg cgccatcatc
     2281 tcggccttcg gcgaaggtgc atccgaaaag gtggaactgc tgcgggaagt gggcgtggcc
     2341 atcgcagcga ctccagcgga gatcggctcg accgtcgcca cgcgtctcaa gaaggcggcc
     2401 tgagggccgc ctttcctcct tcacaacttg atctgaaaga gtccgatgag cttcttcacc
     2461 gtcgaaccgg cgccagcgcg cctcaaccgg agcgaactgg cggttcccgg cagccagccg
     2521 cagatgttcg agaaggcggc gaagtcggcg gccgacgtgg tcttcctcga cctcgaggat
     2581 gcggtcgcgc cggacgacaa ggcccaggcc cgcaagaaca tcatccaggc catcggcgac
     2641 atcgactggg gcagcaaggc gctgtcggtg cgcatcaacg gcctggacac ccattacatg
     2701 taccgcgacg tggtggatgt gctggagcag gcctccgagc gcctcgacct gatcatgatc
     2761 cccaaggtgg gcacggcggc ggacgtctat gccgtcgaca tgctctgcac ccaggtcgag
     2821 acggcgcgcg gccgccgcaa gcgggtgggc ttcgagatga tcatcgagac cgccctcggc
     2881 atgcagaacg tgcatgagat cgcggccgcc tcgaagcgca acgagtcgct tcatttcggg
     2941 gtggccgact atgccgcctc gaccaaggcg cgcaccacct cgatcggcgg cgccaatgcc
     3001 gactatgccg tgctgaccga gcccctggag gacggcagcc gccagaccca ctggggcgac
     3061 atgtggcact atgcgatctc gcgcatggtg gtggcggccc gggccaacgg gcttcgcccg
     3121 gtcgacgggc ccttcggcaa tttctccgac gccgacgcct tcaaggccgc cgcccgccgg
     3181 gccgcggttc tgggctgcga gggcaagtgg gcgatccatc c
//
LOCUS       DBNGVR010000111         1925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_628844, whole genome shotgun sequence.
ACCESSION   DBNGVR010000111 DBNGVR010000000
VERSION     DBNGVR010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_628844"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="AC16A0_02460"
     CDS             <1..906
                     /locus_tag="AC16A0_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104222.1"
                     /translation="HRKRKALRDLAERHRLLEPTMRLRAHAAYATRHVRRRRRHGLPG
                     PLVVSLTSYPPRFPTLHLTLMTLLSQQVLPDRVVLYVFDGDHASLPRDVVALAGDLLD
                     IRRVPTDIRSYKKLVPALGDFPQAFIATADDDVRYRSTWLGELVSAHDPAVREVIGRR
                     AHRIRTDASGRILPYRDWEQRVESTIQADPSILLTGVGGILYPPGVLSPMATDESSFM
                     RLCPSADDVWFYFMARLTGAACRKIGQGPKDYYWEPSQGVALSTFNVEGSGNDEAFAR
                     MVERFGNPLNPGFGGRMVDHDRVSG"
     gene            942..1730
                     /locus_tag="AC16A0_02465"
     CDS             942..1730
                     /locus_tag="AC16A0_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3104223.1"
                     /translation="MKTVDLTFAGRTVALPDLPEYRKFYGKLTSGIWEPHTFEVLARN
                     LDRETVYVDIGAWIGVTPFWASAICKRVIAFEPDPRCFEILRQLSPAYDNVTITQAAL
                     SSEATVTIHAVDGFGSSETSILDIGDGESVTAPGLGKDEIMRRAGGEQAFVKIDIEGY
                     EFAMAAEIAKLRNYNVHGLELAVHPQLLERSLAGSRFSRRIRTAWKVWRLSRIFGDLF
                     PAPRLAKYKSVLSYVVRGVLWKEEPRGADFIFERDRAAAAKETE"
     gene            1727..>1925
                     /locus_tag="AC16A0_02470"
     CDS             1727..>1925
                     /locus_tag="AC16A0_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3104224.1"
                     /translation="MTAGRNDTSGVLIDYVADWLMQKALSGADVEDVFSGFCERLAAA
                     GLPLARVHLSFSRLHPLYRAAS"
BASE COUNT          330 a          595 c          619 g          381 t
ORIGIN      
        1 catcgcaagc gcaaggcgct tcgtgatctc gccgagcgcc atcgcctgct tgagcccacc
       61 atgcgccttc gagcgcacgc cgcctatgcg acccgccatg tccggcgccg ccgccggcat
      121 ggcctgccgg gcccacttgt ggtctcgctg acgtcttatc cgccgcgctt tccgaccctt
      181 catctgactc tgatgacgct gctcagccaa caggtgctgc cggaccgggt tgttctttat
      241 gtttttgacg gtgaccatgc gtccctgccg cgtgatgttg tggccctggc cggcgatctg
      301 ctcgacatcc gccgcgtgcc gacggacatt cgcagttaca agaagcttgt cccggcgctg
      361 ggcgatttcc cgcaggcctt cattgccacc gccgacgatg atgttcgcta tcgttcgaca
      421 tggttgggag aactggtgtc ggcacatgac cctgcagtcc gcgaggtcat cgggcgccgg
      481 gcgcatcgca tcaggaccga tgccagtggc aggatccttc cttaccggga ctgggagcag
      541 cgcgtcgagt cgacgatcca ggccgatcct tccatcttgc tgaccggcgt tggcggtatc
      601 ctctatccgc cgggcgtgct ctcgcccatg gcgacggacg agagcagctt catgcgcctt
      661 tgcccatccg cggacgacgt ctggttctac ttcatggcgc gactcaccgg ggcagcttgc
      721 cgcaagatcg ggcaggggcc gaaagactac tactgggaac cgtcgcaggg cgtcgccctg
      781 agcacattca atgtggaggg cagcgggaac gacgaggcct ttgcccgcat ggtggagcgt
      841 tttggcaatc ccctgaaccc gggattcggc ggacgcatgg tggaccacga ccgggtttcc
      901 ggctaagccg gaccctcggg aacggagtgc tcagccggac gatgaagacc gttgatctga
      961 cctttgccgg gcgcaccgtg gcgcttccgg acctgcctga gtaccgaaag ttttatggaa
     1021 agttgacctc ggggatctgg gaaccccata ccttcgaggt cttggcgcgc aatctcgatc
     1081 gcgagaccgt ctatgtcgac attggggcgt ggatcggagt gacacccttc tgggcgagcg
     1141 ccatctgcaa gcgggtgatc gccttcgaac ctgatcccag atgcttcgag atcctgcgcc
     1201 agctttcacc cgcctatgac aacgtcacga tcacgcaggc cgcgctctcg tcggaggcca
     1261 cggttaccat tcatgctgtc gacggcttcg gcagctcgga aaccagcatt ctcgacattg
     1321 gcgacgggga aagcgtcacg gctccggggc ttggcaagga tgagatcatg cgccgggccg
     1381 gcggtgaaca agctttcgtg aagatcgaca tcgagggcta tgaatttgca atggccgccg
     1441 agatcgccaa gctgcggaac tacaatgtcc acgggctgga gctggccgtt cacccccagc
     1501 tgctggaacg gagcctggcg ggaagcagat tctcgcgccg gatccggacg gcctggaagg
     1561 tctggcggct gagccgcatc ttcggggact tgtttccggc gccccggctc gccaaataca
     1621 agagcgtatt gagctatgtg gtccggggcg tcctgtggaa ggaagagccg cgcggggctg
     1681 atttcatctt tgagcgggat cgggccgcag cggccaagga aacagaatga cagcgggtag
     1741 aaacgacacg tcaggcgtcc tgatcgacta tgtggcagac tggctgatgc agaaggccct
     1801 cagcggcgcg gatgtcgagg atgttttcag tgggttttgc gagagactcg cggcggccgg
     1861 cttgccgctg gcgcgcgtcc acctctcttt ctcgaggctg catccgctgt accgcgctgc
     1921 cagct
//
LOCUS       DBNGVR010000112         2951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5343190, whole genome shotgun sequence.
ACCESSION   DBNGVR010000112 DBNGVR010000000
VERSION     DBNGVR010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5343190"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16A0_02475"
     CDS             complement(<1..815)
                     /locus_tag="AC16A0_02475"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HIR3104225.1"
                     /translation="MNDLAPAALAAVTMDSPSRFINRELSWLGFNMRVLEEARNLHHP
                     LLERLRFLSISGNNLDEFFMVRVAGLAGQVRARVAEISDDGLTPAEQLEQIRRKAQEL
                     MAEQDLRWLQLKEELAEAGIVIVTGPELLPSERDWADQRFLEQILPVVTPIAIDPAHP
                     FPFIPNRGFFICLELKRRKDGGMMKALLPIPQQLDRFIRLPAGDAGSVRFISLEALLT
                     HFIPRLFPGYDVLGEGLVRVIRDSDIEIEEEAEDLVRVFESALKRRRRGSVIR"
     gene            complement(897..1604)
                     /locus_tag="AC16A0_02480"
     CDS             complement(897..1604)
                     /locus_tag="AC16A0_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR3104226.1"
                     /translation="MTMAGSGRQMVLDLPHRPALGREDFLVTDSNRAAVSAIEGHAAW
                     PHHALAIVGPPGSGKSHLIEVWRQMTGAPRIPADGLREADVPQLMREGVLAIDDAPGA
                     QLDERALFHLLNLARQQQGRVLFASLHRPAAWRVGLPDLASRLKAVPYAELGPPDDAL
                     LRGVLVKLFADRQLVVEEPAISFLLLRMQRSLEAARELVAVIDRRALEQKSAITRSFL
                     GKILAETPPPHGPETED"
     gene            complement(1601..2728)
                     /locus_tag="AC16A0_02485"
     CDS             complement(1601..2728)
                     /locus_tag="AC16A0_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3104227.1"
                     /translation="MTLHRQVLIWSAALAALIAVMWLLSAILLPFIAGLVLAYFLDPV
                     ADALERYGLPRLAATGLILLTAILILTTALVILVPVLNDQLARFADDLPSLIESLVAR
                     FDEFAPLWMKELVANSGANVQGSITEVAGKAAGWIVSLLSSILSGGMALVNLLSLMVV
                     TPIVAFYLLVDWDSMIAKVDSWLPRDHADVLRQLARQIDAAMAGFIRGQGSVCLLLGA
                     FYAVALSLAGLKFGLAIGLLAGFLTFIPYAGALIGGVLAIGVALVQFWPDYVSILMIA
                     GIFALGQFIEGNFLSPKLVGKSVGLHPVWLMFALFAFGYVFGFVGLLLAVPMAAAAGV
                     LVRFALGQYLQSPLYLGTGARAAAAVGAPSQPDPPVKRKRR"
     gene            complement(2725..>2951)
                     /locus_tag="AC16A0_02490"
     CDS             complement(2725..>2951)
                     /locus_tag="AC16A0_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3104228.1"
                     /translation="PVKVAPLMISKINTAAQIVFIVGVLGAKAAGMDDGWPITLGAIA
                     VGLLTAASGGAYLMQWIRHMAKGESGADRS"
BASE COUNT          599 a          944 c          957 g          451 t
ORIGIN      
        1 atgcggatca ccgagccgcg gcgccgacgt ttcaaggcgc tctcgaagac gcgtaccaga
       61 tcttcggctt cttcctcgat ttcgatgtcg ctgtctcgga tgacacggac gaggccttcg
      121 cccagcacgt cgtaaccagg gaagaggcgc ggaatgaagt gcgtgagcaa ggcctccagc
      181 gatatgaacc gcacgctgcc ggcgtcgccc gcaggcagac gaatgaagcg gtcaagctgc
      241 tgcggaatgg gcagcagggc cttcatcatg ccgccatcct tgcgccgctt cagttccagg
      301 cagatgaaga agccacggtt ggggatgaac ggaaagggat gtgcaggatc gatggcgatc
      361 ggcgtgacca ccggcaggat ctgctcgaga aaccgctgat cggcccagtc gcgctcgctc
      421 ggaagcagtt cggggccggt gacgatgacg atgccggcct ccgccagttc ctccttcagt
      481 tgcagccagc gaaggtcctg ttcggccatc agctcctgag ccttgcgccg gatctgctca
      541 agctgctcgg ccggcgtcag cccatcgtca ctgatttcag cgacacgggc ccgcacctgg
      601 ccggcaaggc cggccacgcg caccatgaag aactcgtcga ggttgttgcc cgagatggaa
      661 aggaagcgca gccgctccag aagcggatga tgcaggttcc tagcctcctc cagcacccgc
      721 atgttgaagc ccagccatga cagctcccgg ttgatgaagc gggaggggct atccatcgtg
      781 accgcagcga gcgctgccgg tgcgagatcg ttcatgtgct tgtcctggct ttgctccggg
      841 tgaccggcca ttattacgca cttgtgacgg ctccgtgaac acggttctgt tcacccttaa
      901 tcttcggttt caggtccatg aggcggaggg gtttcggcca ggatcttgcc gaggaaggac
      961 cgagtgatcg ccgacttctg ttcgagggcc cggcggtcga tcacggccac cagttcgcga
     1021 gccgcctcca gagacctctg catgcgcagc aagagaaacg agatggccgg ttcctcgacc
     1081 accagttgcc ggtcggcgaa gagcttgacg aggacaccgc gcagcagggc atcgtccggc
     1141 gggccgagct cggcgtatgg aacggctttc agccgcgatg ccaggtccgg gaggccgacc
     1201 cgccaagccg cgggacggtg aagactggcg aagagcaccc tgccctgttg ctgccgggcc
     1261 aggttgagaa gatgaaacag cgctcgctcg tcgagttgcg cgccgggggc atcgtcgatg
     1321 gcaagaacgc cctcccgcat cagctgcggc acgtccgcct cgcgcagacc atcggccgga
     1381 atgcgcggcg caccggtcat ctgacgccag acctcgatga ggtggctctt gcccgatccc
     1441 ggcggcccta caatggccaa cgcatgatgc ggccaggccg cgtggccttc gatggcactg
     1501 actgccgccc ggttcgagtc cgtgacgaga aagtcctccc ggcccagcgc cggcctgtgc
     1561 ggaagatcca gcaccatctg gcggccggag cccgccatgg tcatctgcgc ttccgcttca
     1621 cgggcggatc gggctgcgat ggagcaccta cagctgctgc ggcccgtgcg ccggttccga
     1681 gatagagcgg gctctgcagg tattgtccaa gcgcaaagcg caccagcacg cccgctgccg
     1741 ccgccatcgg cacggccagg aggagaccga cgaaaccgaa gacatagcca aaggcgaaaa
     1801 gcgcgaacat gagccagacc gggtgcaggc cgacgctctt gccgacgagc ttgggcgaca
     1861 ggaaattgcc ctcgatgaac tggccaagcg cgaagatgcc ggcgatcatc aggatggaga
     1921 cgtagtcggg ccagaactgc accagagcca cgccgatggc cagtacgccc ccgatcaggg
     1981 ctcccgcata gggaatgaat gtcaggaacc ctgccagcaa gccaatcgcg aggccgaact
     2041 tcaagccggc aagggacagg gccacggcat agaaggcccc gagcagaagg cagaccgatc
     2101 cctgaccgcg gatgaacccg gccatggccg catcgatctg ccgcgccagc tgccgcagca
     2161 catcggcatg atcgcgggga agccagctgt ctaccttggc gatcatcgaa tcccagtcga
     2221 cgagcagata gaaggcgacg atgggcgtga ccaccatcaa cgacaggaga ttgacgaggg
     2281 ccatgccacc ggacaggatc gacgacagca gcgagacaat ccagcccgct gcctttccgg
     2341 caacttcggt gatcgagccc tgcacgttgg cgccggagtt tgcgacgagt tccttcatcc
     2401 agagcggcgc gaactcgtcg aagcgggcaa ccagcgactc gatcagcgac ggcagatcgt
     2461 cggcaaagcg ggcgagctga tcgttgagga cggggaccag gatcaccagc gccgtcgtca
     2521 gaatgaggat ggccgtcagc aggatgaggc ctgtcgccgc aaggcgcggc aggccatagc
     2581 gctcgagcgc gtccgccacg ggatcgagga aataggcgag taccagaccc gcgatgaagg
     2641 gcagcaggat cgcactgagc agccacatca ccgcaatgag cgcggccagc gctgccgacc
     2701 agatcagcac ctgccgatgc agcgtcatga acggtcagct cccgattcgc ccttggccat
     2761 gtgccgtatc cattgcatga ggtaagcccc gcccgaggcg gcggtcaaca ggccaacggc
     2821 gatggccccg agggttatgg gccagccatc atccatgccg gccgccttcg caccaagaac
     2881 gccgacgatg aagacgatct gggccgcggt gttgatcttg ctgatcatca acggcgccac
     2941 cttgaccggc c
//
LOCUS       DBNGVR010000113         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4950816, whole genome shotgun sequence.
ACCESSION   DBNGVR010000113 DBNGVR010000000
VERSION     DBNGVR010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4950816"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC16A0_02495"
     CDS             <1..510
                     /locus_tag="AC16A0_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104229.1"
                     /translation="RTPDLVPMGIAAVPQSRNVFPTLTVAENLDVGTYAAPPADKSGV
                     LARVLALFPDLAAKLRQPAGELSGGQRQMVAIGRALMSEPRLLLLDEPTAGLSPAYLE
                     RIFDLLLDIRGTGVTILMVEQNARQALRIADHGHVLAAGRNFASGTGAELLADENVRR
                     SFLGGAAAA"
     gene            507..1397
                     /locus_tag="AC16A0_02500"
     CDS             507..1397
                     /locus_tag="AC16A0_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3104230.1"
                     /translation="MIDFINLYLVPGLTIGSVYALGAVGISMIFGILRFAHFAHGDLM
                     TIGAYGVLTAVWFIPLPPLLLLPFGIVLAIVAALAVDRFFYRPLRKLPTIYTVISSFG
                     VALIFRSLAQLTWSSGNQVFVPGVRPPLVLFDTFRIATLHMQVIVITLVIAVALHVFL
                     TRSQLGRAMRAVSDEPELAAVAGLDTERVVRWTWVIGAALAATAGVFAGMDTSMHPNL
                     GWNLLLPMFAAAVLGGIGKPMGAIAGGLIIGVAEETATYTWFSDEAFIPSSYKSAVAF
                     FIMVTLLIFRPQGLFKGRLL"
     gene            1397..>1659
                     /locus_tag="AC16A0_02505"
     CDS             1397..>1659
                     /locus_tag="AC16A0_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3104231.1"
                     /translation="MELTGLLFYLVSVLIAGGIYAILCLALNIQWGMGGLFNAGIAGF
                     FAVGAYTSAIVTTVATEKHLGGFDLPIPFGLAAAAIVAGVTGWA"
BASE COUNT          243 a          556 c          498 g          362 t
ORIGIN      
        1 cgaacgcccg atcttgtacc gatgggtatt gccgccgtgc cacagagccg caacgtcttc
       61 ccgacattga cggtggctga aaacctcgat gtcggcacct atgcggcccc acccgccgac
      121 aagtccggag tcctggcgcg ggttctagcg cttttccccg acctggccgc aaagctgcgc
      181 cagccggcgg gcgagctttc gggaggccag cgtcagatgg tggcgattgg ccgggcgctg
      241 atgagcgaac cccggctcct cctgctcgat gagcccaccg cgggtctctc gcccgcctat
      301 ctcgagcgca tctttgacct gctgctcgac atccgcggga cgggtgtgac catcctgatg
      361 gtggagcaga atgccaggca agccctgcgc attgccgatc acgggcacgt gctggcggca
      421 ggccggaatt ttgccagcgg aacgggtgca gagcttctgg ctgatgagaa cgtgcgccgc
      481 tccttcctcg gaggcgccgc cgcagcatga tcgacttcat caacctctat ctcgtgcctg
      541 ggctgacgat cggcagcgtc tacgcactgg gcgctgtcgg catctccatg atcttcggca
      601 tcctgcgctt tgcccatttc gcgcatggag atctgatgac catcggcgcc tatggcgtgc
      661 tgacggccgt gtggttcatc ccgctgccgc cactgctgct gctgcccttc ggcattgtgc
      721 ttgcaatcgt agcggcactt gccgtcgacc gcttcttcta ccgtccgctg cgcaagctgc
      781 cgacaatcta caccgtgatc tcatcctttg gcgtcgccct gatcttccgt tcgctggcgc
      841 agctgacctg gagttcgggc aaccaagtct tcgtgcccgg cgttcggccg ccgctggtgc
      901 tcttcgatac gttccgcatc gccaccctgc atatgcaggt catcgtcatc accctcgtca
      961 ttgccgtcgc cctgcacgtg tttctcaccc gttcgcagct gggacgcgcc atgcgggccg
     1021 tctcggacga accggaactt gctgccgtgg caggcctcga taccgagcgt gtggtgcgct
     1081 ggacctgggt gatcggcgcg gcactggcgg caacggccgg agtcttcgcc ggcatggaca
     1141 ccagcatgca tcccaatctc ggctggaacc ttcttctgcc catgtttgcc gccgccgttc
     1201 ttggtggcat cggaaagccg atgggcgcca ttgccggcgg tctcatcatc ggggttgccg
     1261 aggagaccgc cacctacaca tggttcagcg acgaggcctt catcccttcc tcctacaagt
     1321 ctgctgtggc cttcttcatc atggtgaccc tgctgatctt ccgaccacag ggcctgttca
     1381 aggggaggtt gctctgatgg aactgacggg gctgctcttc tatttggtga gcgtcctcat
     1441 cgcaggcggc atctatgcga ttctctgcct cgccctgaac atccagtggg gcatgggcgg
     1501 actgttcaat gccggcatcg ccggattctt cgccgtcggc gcctatacat cggccatcgt
     1561 gacgacggtg gccacggaaa aacatctggg cggtttcgac ctgccgattc cctttgggct
     1621 tgccgccgcc gccatcgtcg ccggcgttac cggctgggc
//
LOCUS       DBNGVR010000114         3132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1808638, whole genome shotgun sequence.
ACCESSION   DBNGVR010000114 DBNGVR010000000
VERSION     DBNGVR010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1808638"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="AC16A0_02510"
     CDS             complement(<1..1359)
                     /locus_tag="AC16A0_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3104232.1"
                     /translation="MTDVQPASAPSGRRARGGADARRQARTQTTVRHAGYIQRKIKLY
                     EPFSDDQLELIEHNADTVLQEIGIDFRDDAEALAMWKAAGADVKGDRVRFPRGLVRSL
                     IKTAPREFTQHARNPANSVQVGGNNTVFAPVYGPPFVRDLDNGRRYATIEDFRNFVKL
                     AYMSPSLHHSGGTVCEPVDIPVAKRHLDMVYSHIRYSDKPFMGSVTAPERAEDSVAMA
                     EMVFGKDFVDQNCVLINLINANSPMVFDGTMVGALKVYARANQATVISPFIVAGAMSA
                     VTTVGTITQILAEASVGIAFSQLCRPGAPVVFGTFASSMSMQSGAPTFGTPEPAHVLF
                     GAAQLARRMGVPFRSGGGLCGSKIPDAQAAYESANTLWPTLLGGVNFCLHAAGWLEGG
                     LASSYEKLIMDADQLTMLQKFAEGLDFSENGQALDAMREVGPGSHFLGCSHTQANFET
                     AFW"
     gene            complement(1459..2664)
                     /locus_tag="AC16A0_02515"
     CDS             complement(1459..2664)
                     /locus_tag="AC16A0_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3104233.1"
                     /translation="MRIDVPSRSVRLDPCDPAFFNDPYPYYHEIRSRVPVFRWEEYGH
                     WCFARYEDVNALLRDRRFGRQILHVASREELGWSATPDHLRPFYAFEQHSLLELEPPV
                     HTRLRSLVNRSFLSRQVERLRPAITSLSNGLIDRLEERHEADLLESFATPIPVLVICD
                     LLGVPGDMARQLLSWSHDMVAMYQARRDRAVEDAAVRATIAFSDFMRRYIAERRKSPR
                     DDLLSELICAEEEGRKLSEDELVTTAILLLNAGHEATVHAIGNAAKAFLELGLRDGIG
                     EGHVEEALRYDAPLHLFTRYALEDLDYAGLRLKKGEVVGLLLGAANRDPARFRDPDRF
                     DPGRDPNPQVSFGAGIHFCIGAPLARLEMQIALPLLFKRLPRLALAETPRYRNAYHFH
                     GLERLMVTY"
     gene            2735..>3132
                     /locus_tag="AC16A0_02520"
     CDS             2735..>3132
                     /locus_tag="AC16A0_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HIR3104234.1"
                     /translation="MKVYISVDIEGCAGIAHWDEANKAHADYPEFREQMTRECLAAID
                     GARAAGATGIWVKDAHETGRNLITAMLPEDVTLIRAWAGHPLCMVQELDASFDAVMMI
                     GYHDAAGSEANSLAHTLSSDAVEIRLNGRRA"
BASE COUNT          576 a          937 c         1092 g          527 t
ORIGIN      
        1 ccagaaggcc gtctcgaaat tggcctgggt atgcgagcag ccgaggaagt gcgaaccggg
       61 ccccacttcg cgcatcgcgt ccagcgcctg gccgttctcg gagaaatcca gtccctcagc
      121 gaacttctgc aacatggtca gctggtcggc atccatgatc agcttctcgt aggacgaggc
      181 gaggccgcct tccagccagc cggccgcatg gagacagaaa ttcacaccac cgagcagcgt
      241 cggccacagc gtgttggcgc tttcgtaggc cgcctgtgca tcggggatct tggatccgca
      301 gagcccgccg ccggagcgga agggcacgcc catgcggcgc gccagctggg ccgcgccgaa
      361 gagcacatgc gccggttccg gcgttccgaa ggtgggcgcg cccgattgca tcgacatgga
      421 ggaggcgaag gtgccgaaga ccaccggtgc gccgggacgg caaagctgcg aaaaggcaat
      481 gcccaccgag gcctcggcca gaatttgcgt gatggtgccc accgtggtca cggccgacat
      541 ggcgcccgcc acgatgaagg gcgagatcac ggtggcctgg ttggcgcggg catagacctt
      601 gagcgcgccg accatggtgc cgtcgaagac catgggcgaa ttcgcattga tcaggttgat
      661 cagcacgcaa ttctggtcga cgaagtcctt gccgaaaacc atctccgcca tggccaccga
      721 atcctcggcg cgctccggcg ccgtgaccga gcccatgaat ggtttgtcgg aatagcggat
      781 gtgcgaatag accatgtcga ggtggcgctt ggccaccggt atgtccaccg gttcgcagac
      841 cgtgccgccc gaatggtgca ggctgggcga catgtaggcg agcttcacga agttgcggaa
      901 atcctcgatg gttgcgtagc ggcggccatt gtcgaggtcg cgcacgaagg gcgggccata
      961 gaccggtgcg aagacggtgt tgttgccccc cacctgcacg ctgttggcgg gattgcgggc
     1021 gtgctgggtg aactcgcggg gcgcagtctt gatgaggctg cgcaccaggc cgcgggggaa
     1081 gcggacccgg tcgcccttca cgtcggcgcc ggcagccttc cacatggcaa gggcttcggc
     1141 gtcgtcacgg aagtcgatgc caatctcctg cagcacggtg tcggcattgt gctcgatcag
     1201 ttccagctgg tcgtcggaga agggctcgta gagcttgatc ttgcgctgga tgtaaccggc
     1261 gtggcgcacg gtggtctggg tgcgggcctg gcggcgggca tcggcaccgc cacgggcacg
     1321 gcggccggaa ggagcggatg cgggctggac gtcggtcatg gaggcgcctg ttgagaaagt
     1381 ttcacgtacc tccgtttacg ctcaacaggc cccccgcgtt cgagaggaaa cgtcagccga
     1441 tggtccggaa acgacgcctc agtacgtcac catcaggcgc tccagaccgt ggaaatgata
     1501 ggcattgcgg tagcgcggcg tttcggccag ggccagccgg ggcaggcgct tgaacaggag
     1561 gggcagggcg atctgcatct ccagccgtgc gaggggtgcg ccgatgcaga agtggatgcc
     1621 ggccccgaag ctgacctggg gattgggatc gcggccggga tcgaagcggt cggggtcgcg
     1681 aaagcgggcc gggtcgcggt tggctgcgcc cagcagcagg cccaccacct cgcccttctt
     1741 caggcggagc cctgcatagt cgaggtcctc cagcgcataa cgggtgaaga gatgcagtgg
     1801 cgcatcgtag cgcagcgcct cctccacgtg gccctcgccg atgccgtcgc ggaggcccag
     1861 ttcgaggaag gccttcgctg cattgccgat ggcatggacg gttgcctcgt gtccggcatt
     1921 gagcagcagg atggcggtgg tgacaagctc gtcttcgctg agcttgcggc cttcttcctc
     1981 ggcgcagatc agttcggaca gcagatcgtc gcgcggcgac ttgcgacgct cggcgatgta
     2041 gcgtcgcatg aaatcgctga aggcaatggt ggcccggacc gccgcatcct cgaccgctcg
     2101 gtcgcggcgg gcctgataca tggcgaccat gtcatgcgac caggacagga gttgccgtgc
     2161 catgtcgccg ggtacgccca gcaggtcgca gatcacgagg acgggaattg gcgttgcgaa
     2221 ggattccagc agatcggctt catgccgttc ctcaagacga tcaatcaagc cattggaaag
     2281 gctggtgatt gcaggtcgca atcgctccac ctggcgggac aggaaagagc ggttcacgag
     2341 gctgcgcaga cgtgtgtgca ccggcggctc cagctcaagc agcgagtgct gctcgaaggc
     2401 atagaagggt ctcaggtgat ccggcgtcgc ggaccagccc agttcctcgc ggctggccac
     2461 atgcaggatc tggcgcccga agcgacggtc gcgcagcagc gcattcacat cctcgtagcg
     2521 tgcgaaacac cagtgcccat actcctccca gcggaacacc ggaacccggc tgcggatttc
     2581 gtgatagtag ggatagggat cgttgaagaa cgcgggatcg cagggatcga gcctgacgga
     2641 gcggctgggt acatctatgc gcatggcagc catcttctag cgatctcgcc ggggtgggca
     2701 agtccgtcta tggaggccgc ggagggtcag gatcatgaaa gtctacatca gcgtcgacat
     2761 cgaaggctgc gctggcattg cccattggga cgaggccaac aaggcccatg ccgactatcc
     2821 ggagttccgc gagcagatga cgcgggagtg tcttgccgcc atcgatggcg cgcgcgcggc
     2881 cggcgccacc gggatctggg tcaaggatgc gcatgagacc gggcgcaacc tgatcaccgc
     2941 gatgctgccg gaagacgtga cgctgatccg cgcctgggcg ggtcatccgc tgtgcatggt
     3001 gcaggaactc gacgcgagtt tcgatgcggt catgatgatc ggctatcatg atgccgcagg
     3061 ctccgaggcg aatagcctgg cacataccct gtccagcgat gcggtcgaga tccgcctcaa
     3121 tggcaggcgg gc
//
LOCUS       DBNGVR010000115         2954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_236033, whole genome shotgun sequence.
ACCESSION   DBNGVR010000115 DBNGVR010000000
VERSION     DBNGVR010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_236033"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC16A0_02525"
     CDS             <1..476
                     /locus_tag="AC16A0_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR3104235.1"
                     /translation="LSLGLSNCRPTSTGAVEIQSPDPFTHPRLTFHAYGTDRDAAEIV
                     AAVKFLRVIAAQAPFQGLMAEELRPGPAVRTDAELLHDARQRSGTVYHHACTCRMGPD
                     PASAVVDARLRVHGTEHLRVIDASAFPNLIAGNTNAPTMMLGWRGAAMVLDDARH"
     gene            619..2787
                     /locus_tag="AC16A0_02530"
     CDS             619..2787
                     /locus_tag="AC16A0_02530"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3104236.1"
                     /translation="MVSLNRRKASLALLVALPIAIASLAMLWSSHRMLNGIAQSVDQQ
                     EAKRTWQAVKSAMASAQERLAGTVADNAHWDDAVLQSYGKIDPDWMYDTWGVGSDDIN
                     YDTMYLVDAKGDVLTAFQNGEVFDVPADTYFGRALERTLSERPMNGENFGVLSSLVET
                     PQGLAVMAAAPILATSDDIRIPTPRPNVLIFSKQLTAPLLAKMSEQYIVDALAIAPIR
                     SRAEMASDTALLDRWGNPVALASWQARHPGDLASQSYRFSALATILALIGVMTPLAFS
                     YGRVISRMENNERKARLASRHDALSGLPNRAFLLDELGARLAQAKPAELALIFIDLDG
                     FKAINDAYDHETGDKLIRAVAAGLQSVVAGDGLIARLGGDEFAVLVTGESAAKRGEQI
                     ARRVLAFVKEPFDIDGRIASIGASIGIAELGSEPLDPAELMRRADIAMYDAKDNGRNR
                     WRRFDATLDLKRIEDLTIANELRAFIARGEFDVAYQPMVDSRDRSIVGVEALARWPKS
                     SPREMPPDRFIPVAEEHGLIDSLSALILGIACRDMVQWHDLKLAVNISPVQIANPQLV
                     PDIIRIAGEAGLPLHRLEVEFTESVLIRNPARAKQVIRELQGLGVTVALDDFGTGYAS
                     VGYLREYGFNKIKLDRSLTHSISTDIAAQQVVQGTILIAKGLSANIIAEGVETEEEAQ
                     LMRLSGCNQLQGFYFGRPQAATALGSLLGSETAEPLRETA"
BASE COUNT          523 a          969 c          972 g          490 t
ORIGIN      
        1 gcctgtcact gggcctctcc aactgccgcc cgaccagcac gggtgcggtg gaaattcagt
       61 cacccgatcc cttcacccat ccgcgcctga cattccatgc ctatggcacg gatcgcgatg
      121 ccgccgagat cgttgcggcg gtgaagttcc tgcgggtgat tgcggcgcag gcgcccttcc
      181 agggtctgat ggccgaagag ctgcggccgg ggccggcggt caggactgac gcggaactcc
      241 tgcatgatgc ccgccagcgc agcggcacgg tctatcatca cgcctgcacc tgccgcatgg
      301 gaccggatcc tgcctccgcc gtggtggatg cccggctgcg cgtgcatggc accgagcacc
      361 ttcgcgtgat cgacgcctcg gcctttccca acctgatcgc gggcaatacc aatgcgccca
      421 ccatgatgct gggctggcgc ggcgccgcca tggtgctgga cgacgccagg cactgacgcc
      481 tgagacacgt gcggcgcgag ctgtcacgcc gacagggaaa ccgcaagaca ctgttaacca
      541 acagtgctga ttcacccaga tggttcagct tcctttaggg cgcgcgtgtt tctcatgcgt
      601 tggggaaaag cacgaacaat ggtcagtctc aatcgcagaa aagccagtct cgcccttctg
      661 gtcgccttgc cgatcgccat cgcgtcgctc gccatgctgt ggagttccca ccggatgctc
      721 aatggcattg cgcagagcgt tgatcagcag gaggccaagc gcacctggca ggccgtgaag
      781 tccgccatgg cgtccgcgca ggagcgcctg gccggaaccg tggccgacaa tgcgcattgg
      841 gacgatgcgg tgctgcagtc ctacggcaag atcgatccgg actggatgta cgacacctgg
      901 ggcgtgggca gcgacgacat caactacgac accatgtatc tcgtcgatgc gaagggcgac
      961 gtcctcacgg ccttccagaa tggcgaggtc ttcgatgttc ccgccgacac ctatttcggc
     1021 agggccctgg agcggacgct cagcgagcgt cccatgaacg gcgagaactt cggggttctg
     1081 agttcgctgg tcgagacgcc gcaggggctt gcggtgatgg cggcggcgcc gatcctggcg
     1141 acgagcgacg acatccggat cccgactccg cggccgaacg tgctgatctt ctcgaagcag
     1201 ctcacggcgc cgcttctggc caagatgagc gaacaataca ttgtcgacgc acttgccatt
     1261 gcaccgatcc ggtcgcgcgc ggagatggcc tcggacacgg ccctgctgga ccgctggggc
     1321 aatccggtgg cgctcgccag ctggcaggcc cggcaccctg gcgatctcgc cagccagtcc
     1381 taccgcttca gcgccctggc gaccatcctg gcgctcatcg gcgtcatgac cccgcttgcc
     1441 ttctcttatg gccgtgtcat ctcgcggatg gagaacaatg aacgcaaggc gcggctggcc
     1501 tcgcgtcacg atgccctctc gggcctgccc aaccgcgcct tcctgctcga cgagctgggc
     1561 gcgcggctgg cgcaggcgaa gcccgcggaa ctggcactca tcttcatcga cctcgacggg
     1621 ttcaaggcga tcaatgatgc ctatgatcac gagaccggcg acaagctgat ccgggccgtc
     1681 gcggcggggc tgcagagcgt ggtggccggc gacggactca tcgcgcgcct gggtggcgac
     1741 gagttcgccg tgctggtgac cggagagtcg gcggcaaagc gcggcgagca gattgcgcgg
     1801 cgtgtgctgg ccttcgtgaa ggagcccttc gacatcgacg gccgcatcgc cagcatcggc
     1861 gccagcatcg gcattgccga actgggcagc gagccgctcg acccggccga gctgatgcgg
     1921 cgggccgaca tcgcgatgta tgacgcaaag gacaatgggc gcaaccgctg gcgccgcttc
     1981 gacgcaacgc tggacctgaa gcgcatcgag gatctcacca tcgcaaacga gctgcgggcc
     2041 ttcatcgcgc gcggcgagtt cgacgtggcc taccagccca tggtcgattc gcgcgaccgg
     2101 tcgatcgtgg gcgtggaggc attggcccgc tggcccaagt cgtcgccgcg cgaaatgccg
     2161 cctgaccgct tcatcccggt ggccgaggag catggcctga tcgacagtct cagcgcattg
     2221 atcctcggca ttgcctgccg tgacatggtg cagtggcatg acctcaaact ggccgtcaat
     2281 atctccccgg tgcagatcgc caatccgcag ctggttcccg atatcatccg aattgccggc
     2341 gaggcggggc tgccgcttca ccgcctcgaa gtggaattca ccgagtcggt gctgatccgg
     2401 aacccggcgc gggcgaagca ggtgatccgg gagctccagg gccttggcgt gaccgtcgcg
     2461 ctggatgact tcggcaccgg atatgccagc gtgggatacc tgcgcgaata tggcttcaac
     2521 aagatcaagc tggaccgctc gctcacccat tcgatctcga cggacatcgc cgcccagcag
     2581 gttgtgcagg gcacgatcct gatcgccaag ggcctgtcgg caaacatcat cgccgagggc
     2641 gtggagacgg aagaggaagc ccagctgatg cggctttccg gctgcaacca gctgcagggc
     2701 ttctatttcg gccggccgca ggccgccacg gccctgggca gcctactcgg aagcgagacc
     2761 gccgagccgc tccgggagac ggcctgacct gcgctcacgg cgaaccttca gcgcggtgcg
     2821 cgccggggcg ggcgaaacgg taccacaggc gccaggcttc gagcaggacg agggggacga
     2881 aatgcgcgag cgccggcgtg ttgtcgagct tcatccagat ccaatgcagg aagacggcca
     2941 gagccgcgag atag
//
LOCUS       DBNGVR010000116         4313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4951750, whole genome shotgun sequence.
ACCESSION   DBNGVR010000116 DBNGVR010000000
VERSION     DBNGVR010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4951750"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="AC16A0_02535"
     CDS             complement(<1..654)
                     /locus_tag="AC16A0_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3104237.1"
                     /translation="MSGNRNILRTSAAAFALGLVMWGASPSAVTPAAAQTVELPSIAD
                     LTDKLLPAVVEITIETKGPGTTVAPSMPELPDNSPFKDFFEDFFKRQQEGDGQPRTMT
                     SMGSGFVIDPAGLIVTNNHVVEGAESIEVHLQDDTVLKAEVVGRDPKTDLAVLRVKPA
                     APLASVALGNSDTLRIGEWVVAIGNPFGLGGSLSLGIVSARNRDINAGPYDDFIQTDA
                     "
     gene            complement(676..873)
                     /locus_tag="AC16A0_02540"
     CDS             complement(676..873)
                     /locus_tag="AC16A0_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HIR3104238.1"
                     /translation="MMPALFTALGLVLVIEGLLYALVPGQLRRMAELLRQVTDDQLRI
                     GGASAIALGVLIVWITRSVSG"
     gene            complement(870..1763)
                     /gene="hflC"
                     /locus_tag="AC16A0_02545"
     CDS             complement(870..1763)
                     /gene="hflC"
                     /locus_tag="AC16A0_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR3104239.1"
                     /translation="MSTTKTILAIIAAAIAIILYMSYFVVDERQKALVLRFGEINRIV
                     EEPGLYFKLPIADTVTPIDDRIIIWENNDRPVQDVASQVYIVDAITLARIKDARLFRE
                     TLGADLMQAEARVAARLDAALRQTYGRRSFDAALSSDRATMMREIRDELRTEADLLGI
                     EIVDVRVRRTDLSENVLEQTYRRMESERNALAQDIRSQGEATKTRMNAETDREVTVKI
                     AEARRQSEIIRGEGDAERNRVFAEAFEQDPEFFSFYRSMQAYAKSLASQGTTMVLNPD
                     SEFFKYFGTQQRTPEAAAPAQ"
     gene            complement(1782..2867)
                     /gene="hflK"
                     /locus_tag="AC16A0_02550"
     CDS             complement(1782..2867)
                     /gene="hflK"
                     /locus_tag="AC16A0_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR3104240.1"
                     /translation="MPWNSEGGGGNQGPWGQGPWGQGGGPKRPSGGGGGGRGPTPPDL
                     DELIRKSQDKLKQVIPGGGGGRFAWIIPILILAGFIGVNSVYQVQPDERGIVLRFGQY
                     NRTAEPGLHFALWPIERMEKPKVGTVRQINIGSEGNQGQMLTSDKNIINVPFSVFWRI
                     NDPRQFLFNVQAPEETIRAVSQSAMREIVGQTRAQAILTTGKNTVEAQVMEITQNLLN
                     EYNAGVTITQINLGDVQPPREVANAFAEVVRAGQNQQQLINEAEQYRNQQSRMAEGEA
                     AKLVEDAKGYKARVIADAQGEAARFISVYEQYRNAKDVTRERIFLETMEQVLGETNKV
                     LIEGGQGGSGVVPYLPLPEIQKRATGN"
     gene            complement(2932..4221)
                     /locus_tag="AC16A0_02555"
     CDS             complement(2932..4221)
                     /locus_tag="AC16A0_02555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HIR3104241.1"
                     /translation="MSQVPSNPDVVIIGAGAAGIGAGIGLLRHGVSFAIIEAKQRIGG
                     RAYSETSSLGHLWDQGCHWFHTADRNVLRSIADSLGHGYRKEPYGGGFKAFQDGRWED
                     VSIRDAYVWTMLDYIAAEGAKADVSAESLLDRSHPGYSSIRHWCQLMQSHDPENISAK
                     DVSSYLDTHINLAVRDGYGALVARLATGLPIRLGTAARRIAVTGSGVAVETDAGTLRA
                     AACIVAVPARMMERERIAFAPGLPPAVQDAFSNVPMGHCEKVAVAFDAPVLSELGVGY
                     ADVLEDKAAGLPPFNIEVHPFGRPIIVAHMGGSAVAEMTPEDRLDLFGRVVTRAFGAD
                     VSRRITHRTTTSWTADPFINGAYSCAKPGKAHLRAVFDEPVHDRVFLAGEHVHRYFHA
                     TAHGAYETGVAAAARAARLLGRPVLAGEPEWLPPNHL"
BASE COUNT          727 a         1379 c         1395 g          812 t
ORIGIN      
        1 ggcatccgtc tggatgaagt catcataggg gccggcattg atgtcgcggt tgcgcgcaga
       61 gacgatgcca agcgacaagg atccaccgag gccgaaggga ttgccgatgg ccacaaccca
      121 ttcaccgatc cgcagggtat cgctgttgcc cagcgccacg gaggccaggg gtgccgccgg
      181 cttcacccgc agcacggcca agtcggtctt gggatcgcgg cccaccacct cggccttcag
      241 cacggtatcg tcctgaagat gaacctcgat ggactccgcg ccctcgacga cgtgattgtt
      301 ggtgacgatc aggccggccg ggtcgatcac gaatcccgaa cccatggagg tcatggtcct
      361 gggctgcccg tcgccttcct gctggcgctt gaagaagtcc tcgaagaagt ctttgaaggg
      421 agagttgtcc ggcagttccg gcatgctggg tgccacggtc gtgccaggcc ccttcgtctc
      481 gatggtgatc tcgaccacgg cgggcagaag cttgtcggtc aggtcggcaa tggagggcag
      541 ttcgacggtc tgcgccgcgg ccggggtgac ggcagatgga ctggcgcccc acatcacgag
      601 gcccagcgca aaggccgctg ccgaagtgcg aagaatgttc cgattgccgc tcatcatccg
      661 tctccaatcg cccgattatc ccgatacgct ccgcgtgatc cagacgatca gtacgccaag
      721 ggcgatggcg cttgcgccgc cgatgcgaag ctggtcatcc gtcacctggc gcaggagctc
      781 cgccatgcgc ctcaactgcc ccggaaccaa ggcataaaga aggccctcga tcacgagtac
      841 gagaccgagg gccgtgaaca gtgccggcat cattgtgcgg gcgcagcggc ctccggagtc
      901 cgctgctgcg tgccgaagta cttgaagaac tccgagtccg gattgagcac catggtggtg
      961 ccctggctgg ccaggctctt cgcataggcc tgcatcgagc ggtagaacga gaagaactcg
     1021 ggatcctgct cgaaggcctc ggcaaagacc cggttgcgct cggcatcgcc ttcgccccgg
     1081 atgatctccg actggcggcg ggcctcggca atcttcaccg tcacctcgcg gtcagtttcg
     1141 gcattcatgc gggtcttggt cgcttcgccc tgggaccgga tgtcctgcgc cagcgcgtta
     1201 cgctcggatt ccatgcgccg gtaggtctgc tccagcacgt tctcgctgag gtcggtgcgg
     1261 cgcacccgga cgtcgacgat ctcgataccc agaagatcgg cttcggtgcg cagctcgtcg
     1321 cggatctcgc gcatcatggt ggcacggtcg gatgacagcg cggcatcgaa ggagcggcgg
     1381 ccataggtct gccgcaaggc cgcgtcgaga cgcgccgcga cgcgggcttc cgcctgcatc
     1441 aggtcggcgc ccagggtttc gcggaacagg cgggcatcct tgattcgcgc cagcgtgatg
     1501 gcgtcgacga tatagacctg gctggccacg tcctgaaccg ggcggtcgtt gttttcccaa
     1561 atgatgatgc ggtcatcgat gggcgtcacc gtgtcggcga tcggcagctt gaaatagagg
     1621 ccgggctcct cgacgatgcg gttgatctcg ccgaagcgca ggaccagcgc cttctgccgc
     1681 tcgtcgacga cgaagtacga catgtagagg atgatcgcga tggccgcggc gatgatggcc
     1741 aggattgtct tggtggtgct catgatcgtg tcatgtcctc gtcaattgcc ggtggcgcgc
     1801 ttctggatct cgggcagcgg cagatagggg acaaccccgg atccgccctg gccaccctcg
     1861 atcaggacct tgttggtctc gcccagcacc tgctccatgg tttccaggaa gatgcgttcg
     1921 cgggtcacgt ccttggcgtt cctgtactgc tcgtagaccg agatgaagcg ggccgcctcg
     1981 ccctgcgcat cggcgatcac acgggccttg tagcccttgg catcctcgac cagcttcgcc
     2041 gcctcgcctt cggccatgcg gctctgctgg ttgcggtact gttcggcctc gttgatgagt
     2101 tgctgctggt tctgcccggc gcgcaccact tcggcgaagg cattggccac ctcgcgtggc
     2161 ggctgcacgt cgcccaggtt gatctgcgtg atggtgactc cggcattgta ttcgttgagg
     2221 aggttctggg tgatttccat cacctgcgct tccaccgtgt tcttgcccgt cgtcagaatt
     2281 gcctgcgccc gtgtctggcc gacgatctcg cgcatggcgc tctgcgagac tgcgcgaatg
     2341 gtctcttccg gggcctgaac gttgaagagg aactgccgcg gatcgttgat gcgccagaac
     2401 accgagaagg gcacgttgat gatgttcttg tcgctggtca gcatctggcc ctggttgcct
     2461 tcggagccga tgttgatctg gcggacggtg ccgaccttcg gcttctccat gcgttcgatc
     2521 ggccagaggg cgaagtgcag gcccggctcg gcggtgcggt tgtactggcc gaagcgcagc
     2581 acgatgccgc gctcgtccgg ctgaacctga tagacgctgt tgacgccaat gaagccggcg
     2641 agaatgagaa tcggaatgat ccaggcaaac cggccgccgc ccccaccggg aatgacctgc
     2701 ttcagcttgt cctgactctt gcggatgagt tcatcgaggt ccggaggcgt gggtccacgg
     2761 ccaccgccgc cgccgccact cgggcgcttc ggtccgccac cctgccccca cggcccctgg
     2821 ccccacgggc cctggtttcc gccaccgcct tctgaattcc acggcattgc ttttcaccta
     2881 ttctcgtcgt ggtttcgatc tgggggtcat atagttagcc tagccccccg gctacaagtg
     2941 atttggcggt aaccattccg gttcgccggc caggaccggt cggcccagca gccgggcggc
     3001 cctggcggct gcggcgacac ccgtttcgta ggcgccatgt gccgtggcat ggaagtatcg
     3061 gtggacgtgt tcgcccgcca ggaacacgcg atcgtgaacc ggctcgtcga aaaccgcgcg
     3121 aagatgggcc tttcccggct ttgcgcagga ataggcgccg ttgatgaagg ggtcggcggt
     3181 ccagctggtg gtggtccgat gcgtgatccg acggctgaca tcggcgccga aggcccgcgt
     3241 cacgacccgg ccaaagagat cgaggcgatc ctccggggtc atctctgcca ccgcactgcc
     3301 ccccatgtgg gcgacgatga tcggccggcc gaagggatgg acctcgatgt tgaacggcgg
     3361 cagcccggct gccttgtctt caaggacatc ggcatagccg acgcccagct ccgacagcac
     3421 gggggcgtcg aatgcgacgg cgaccttctc gcaatggccc atcggcacat tgctgaaggc
     3481 gtcctgcacg gccgggggca ggcccggcgc gaaagcgatg cgttcccgct ccatcatccg
     3541 ggccggcacc gcgacgatgc aggccgcagc gcgcagcgtt cccgcatcgg tttcaaccgc
     3601 gacgcccgac cccgtcacgg caattcgacg cgcggccgtg ccgaggcgga tcggaagacc
     3661 agtggccagc cgcgccacca gcgccccata tccgtcgcga acggcaaggt tgatgtgcgt
     3721 gtccagatag ctgctgacgt ccttcgcgga gatgttttcg ggatcatgcg actgcatgag
     3781 ctggcaccaa tgccgtatcg acgagtagcc gggatgtgac ctgtccagca ggctttcggc
     3841 cgagacatca gccttggcac cctccgcggc aatgtagtcc agcatggtcc agacataggc
     3901 gtcgcggatg ctgacatcct cccaccgtcc atcctggaag gccttgaagc cgccgccata
     3961 gggttccttg cgatagccat gaccaaggct gtcggcgatg gaccggagaa cattgcgatc
     4021 agccgtatga aaccagtggc agccctggtc ccagaggtgg cccagacttg aggtttcact
     4081 ataggcccgg ccgccaatac gctgttttgc ctcgataatg gcaaaggaaa caccgtgacg
     4141 gagcaagccg attccggccc cgatgccggc tgcgccggca ccgatgatca caacatcggg
     4201 attcgaagga acttgactca tcgttcaact ctggccgggc ggccggcgcc ggtcaagcac
     4261 gcgcaggatg aagcccgcgc tgtcgccctc ggccttggga tggacctcgc ggg
//
LOCUS       DBNGVR010000117         2510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1809689, whole genome shotgun sequence.
ACCESSION   DBNGVR010000117 DBNGVR010000000
VERSION     DBNGVR010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1809689"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="AC16A0_02560"
     CDS             <1..274
                     /locus_tag="AC16A0_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3104242.1"
                     /translation="LEKLIDMVLSVEDVGIYKPSRRVYRHAMQKLQLHDAPSICFVSA
                     NGWDAQAASQFGFQAVRVNRFGLADDRIPGKPAALIADLGSLAALI"
     gene            complement(283..1059)
                     /locus_tag="AC16A0_02565"
     CDS             complement(283..1059)
                     /locus_tag="AC16A0_02565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3104243.1"
                     /translation="MSYLPLVGLPADTYESHGFLYHSIGDKYVRAVAEVANCSPVMIP
                     AIMDALHLDALLDRFDGILMTGAVSNVHPPHYGEQPSADHEPYDHARDQLTLRLITRT
                     IERGIPLFCICRGFQELNVVMGGTLETELQRNEGRLDHRAPKHDDVDVRYAPAHLINI
                     RPGGMLERILGKRETMVNSIHRQGIKRLAPRLAVEATAPDGIIEAVSVREAKTFAFGT
                     QWHPEFKAMNNPDSVKLFTAFGDAVRAHASARSAPLAKIA"
     gene            1126..1896
                     /locus_tag="AC16A0_02570"
     CDS             1126..1896
                     /locus_tag="AC16A0_02570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104244.1"
                     /translation="MADVIAAKDLFDLTGQAVLVTGASSGLGNRFARVLAAHGATVVA
                     AARRGDRLAALAASDPRIHPLVLDVTDRASLGAALDAAEGLAGPLTLLVNNAGVASGG
                     RIVDAAADEFRRVQEINVDAVWHLAQIFAQRCIARGSQGAVINIASMVSYKVGLSSAA
                     YAVSKAAVLQMTKAMALEWARHGIRVNAIAPGFIHSEMTDGYLASPAGREMIKRIPQR
                     RAGEASDLDGTLLLLASPRASGFITGSSILVDGGQLLS"
     gene            1906..>2510
                     /locus_tag="AC16A0_02575"
     CDS             1906..>2510
                     /locus_tag="AC16A0_02575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3104245.1"
                     /translation="MDFTLPPEIDALRLKVRAFVAEHVLPLEADKANFDAYENIRLDV
                     LEPLRARARAAGLWAPQMPIARGGLGLPMVGWCAFYEEANRSIFGPLAFNCAAPDDGN
                     MNVLNKVLKTEAEKDRWLQPIIDGKVRSSFVMTEPHPGSGSDPGGMMLTRAEKRGEKY
                     IVHGRKWFISGADGASHFILVARTSDDPRRGLSAFLFHRDQP"
BASE COUNT          403 a          816 c          849 g          442 t
ORIGIN      
        1 gctggaaaaa ctgatcgaca tggtgctgtc ggtcgaggac gtcggcatct acaagccgag
       61 ccggcgggtc tatcgccatg ccatgcagaa gctgcagctt cacgatgcgc cgtcgatctg
      121 tttcgtgtcg gccaatggct gggacgccca ggccgcgtcg cagttcggat tccaggccgt
      181 gcgcgtcaac cgcttcggcc tggcggatga ccgcattccc ggaaagcccg ccgcgctgat
      241 cgccgatctc ggcagcctcg ctgcgttgat ctgatccctg tctcaggcga tcttcgccag
      301 cggggcgctg cgtgcggagg catgggcacg aaccgcatcg ccgaaggcag tgaagagctt
      361 caccgaatcc ggattgttca tggccttgaa ctcgggatgc cactgcgtgc cgaaggcaaa
      421 ggtcttggcc tcgcgcaccg agaccgcctc gatgatgccg tcgggtgcgg tggcctcgac
      481 cgcgagccgc ggcgccagcc gcttgatgcc ctggcggtgg atcgagttca ccatggtctc
      541 gcgcttgccg aggatccttt ccagcatgcc gccggggcgg atgttgatga gatgcgcggg
      601 tgcgtagcgc acgtcgacgt cgtcatgctt gggcgcgcgg tggtcgaggc ggccctcgtt
      661 gcgctggagt tcggtctcca gcgtgccgcc catgacgacg ttcagctcct ggaatccgcg
      721 gcagatgcag aagagcggga tgccgcgctc gatggtgcgg gtaatgaggc gcagcgtgag
      781 ctggtcgcgc gcatggtcat agggctcatg gtcggccgag ggctgctcgc cgtaatgcgg
      841 cggatgcacg ttcgagaccg cgccggtcat caggatgccg tcgaacctgt cgagcagcgc
      901 gtcgagatgc agcgcgtcca tgatcgcggg gatcattacc ggcgagcagt tcgccacctc
      961 ggccacggcg cgcacatatt tgtcgccgat cgaatggtag aggaacccat ggctttcgta
     1021 ggtatcggcg ggcaggccga cgaggggcag gtatgacatg gcaaagcctt gtgatgaccg
     1081 tgatttggcg tctagatagc ccatccggcg aggaaaagcc aattgatggc cgatgtcatt
     1141 gcagccaagg accttttcga cctgaccggc caggcggtgc tggtgacggg cgcatcctcg
     1201 ggccttggca accgcttcgc ccgtgtgctg gcggcccatg gcgccaccgt ggtggcggcg
     1261 gcgcggcgcg gcgaccggct ggcggcgttg gccgcctccg atccccgcat ccatccgctg
     1321 gtgctggacg tgacggaccg ggcctcgctc ggtgcggcac ttgacgctgc cgaagggctc
     1381 gcgggcccgc tcaccctgct cgtcaacaat gccggcgtgg ccagcggcgg acgcatcgtc
     1441 gatgccgcgg cggacgagtt ccggcgggtg caggagatca acgtcgatgc ggtgtggcat
     1501 ctggcccaga tctttgcgca gcgctgcatc gcgcgcgggt cgcagggtgc tgtgatcaat
     1561 atcgcgtcca tggtcagcta taaggtcggc ctctcctcgg ctgcctatgc cgtcagcaag
     1621 gccgcggtgc tgcagatgac caaggccatg gcgctggaat gggcgcgcca cggtattcgc
     1681 gtcaacgcca tcgcgccggg cttcatccac agcgaaatga cagacggcta tctcgccagt
     1741 cccgcgggcc gggagatgat caagcgcatc ccgcagcgcc gggcgggtga agcctccgat
     1801 ctcgacggca ccctgctgct gctcgccagc ccgcgcgcct cgggcttcat cacggggtcg
     1861 agcatcctgg tcgacggcgg acaattgctg agctgagaga cagtcatgga ctttaccctt
     1921 ccgcccgaga tcgacgcgct ccgcctcaag gtccgtgcct tcgtcgccga acatgtgctg
     1981 ccgctcgagg cggacaaggc caacttcgac gcttacgaga acatccggct cgatgtgctc
     2041 gaacccttgc gcgccagggc gcgggcggcg ggactctggg cgccgcagat gcccatcgcg
     2101 cgcggcgggc tgggcctgcc catggtcggc tggtgcgcct tctacgaaga ggccaaccgc
     2161 tcgatcttcg gcccgctcgc cttcaactgc gcagcccccg acgacggcaa catgaacgtc
     2221 ctgaacaagg tgctgaagac ggaggccgag aaggaccgct ggcttcagcc catcatcgac
     2281 ggcaaggtcc gctcctcctt cgtcatgacc gagccgcatc ccggatcggg ctcggacccc
     2341 ggcggcatga tgctgacgcg cgccgagaag cgcggcgaaa aatacatcgt acacggacgg
     2401 aaatggttca tttccggcgc cgatggcgcg tcgcacttca tcctcgtcgc ccgcacctcg
     2461 gacgatccgc gccgcgggct ctcggccttt ctctttcacc gcgatcagcc
//
LOCUS       DBNGVR010000118         2232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_434553, whole genome shotgun sequence.
ACCESSION   DBNGVR010000118 DBNGVR010000000
VERSION     DBNGVR010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_434553"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /gene="glgC"
                     /locus_tag="AC16A0_02580"
     CDS             <1..1196
                     /gene="glgC"
                     /locus_tag="AC16A0_02580"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR3104246.1"
                     /translation="SRLMEMTDKRAKPAVPFGCKSRIIDFALSNALNSGIRRIGVATQ
                     YKAHSLIRHMQRGWNFFRPERNESFDILPASQRVSEDQWYAGTADAVYQNLDIIMSYG
                     PKYMVILAGDHIYKMDYELMLQQHVDSGADVTIGCLEVPRMEATGFGVMAVDTKDRIT
                     AFVEKPKDPPGIPGAPDMALASMGIYVFETAALADHLRRDAADKNSSRDFGKDIIPHI
                     VKHGKAVAHRFTNSCVRSAQETEAYWRDVGTVDAYWEANIDLTETLPALDLYDQNWPV
                     WTYSEVTPPAKFVHDLEGRRGNAVASLVSGGCIVSGASIKRAMLFTNVRVNSFSSLEE
                     AVVLPNVNIGRNVSLKKVVIDSRVDIPEGLVVGEDPELDAKRFRRTASGVCLITKPMI
                     DRLAT"
     gene            1193..>2232
                     /locus_tag="AC16A0_02585"
     CDS             1193..>2232
                     /locus_tag="AC16A0_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR3104247.1"
                     /translation="MKVLSAAAEIYPLVKTGGLADVVGALPAALAGQGVAMRCLVPGY
                     RPVMSALGGIAVLHRYADLFGGPASLVAGKAGGLDLIAIDAPHLYDRPGNPYLGPDGK
                     DWPDNAQRFAALSRAAAEVARGIIPGYQPDVLHLHDWQAALASVYVKFLGGPKTLLTV
                     HNIAFQGQFPASIFASLQLPAAAFAIDGVEYYGSVSFLKGGLATANAISTVSPSYAQE
                     ICTPDYGMGLDGLLRARHAVLSGIVNGIDAAVWNPADDRGIAATYTAKTLNRRRANKR
                     EVERRFGLEEGDGILHAVVSRLTWQKGMDIFVESLDEVVASGARLALIGTGEAPLEAS
                     IQAASQRHKGRIG"
BASE COUNT          398 a          767 c          689 g          378 t
ORIGIN      
        1 gcagccgcct catggaaatg accgacaagc gggccaagcc cgccgtgcct ttcggctgca
       61 agtcccgcat catcgacttc gcgctgtcca atgccctgaa ctcgggcatc cgccgcatcg
      121 gggtcgccac ccagtacaag gcgcacagcc tgatccgcca catgcagcgc ggctggaact
      181 tcttccggcc ggagcgcaac gagagcttcg acatcctgcc ggcctcgcag cgcgtctcgg
      241 aagaccagtg gtatgcgggc acggccgacg cggtctacca gaacctcgac atcatcatga
      301 gctacggtcc gaaatacatg gtgatcctgg ccggagacca catctacaag atggactacg
      361 agctgatgct gcagcagcac gtcgactcgg gcgccgacgt caccatcggc tgcctcgagg
      421 tgccgcgcat ggaggccacc ggcttcggcg tgatggccgt cgacaccaag gaccgcatca
      481 cggccttcgt ggagaagccg aaggatccgc ccggcattcc cggcgcgccc gacatggcgc
      541 tggcctcgat gggcatctat gtcttcgaga ccgctgcgct ggccgatcat ctccgccgcg
      601 atgccgccga caagaactcc agccgcgact tcggcaagga catcatcccg cacatcgtca
      661 agcacggcaa ggccgtggcg caccgcttca ccaattcctg cgtgcgctcg gcccaggaga
      721 cggaggccta ctggcgggat gtcggaaccg tcgatgccta ttgggaggcc aacatcgacc
      781 tcaccgaaac gctccccgcg ctggacctat acgatcagaa ctggccggtc tggacctaca
      841 gcgaagtgac accgcccgcc aagttcgtgc atgacctcga aggccggcgg ggcaatgccg
      901 tggcctcgct ggtctcgggt ggctgcatcg tgtcgggcgc ctcgatcaag cgcgccatgc
      961 tcttcaccaa tgtgcgggtg aactccttct cgtcgctcga agaggcggtg gtgctcccca
     1021 acgtgaatat tggtcgaaat gtttcgctca agaaggtggt gatcgacagc cgcgtcgaca
     1081 tccccgaggg actcgtcgtc ggagaggatc cggaactcga tgccaagcgc ttccgccgca
     1141 ccgccagcgg tgtctgcctc atcaccaagc ccatgatcga tcgcctcgcc acatgaaggt
     1201 cctctcggca gccgcggaaa tctacccgct ggtgaagacc ggcggccttg ccgacgttgt
     1261 gggcgcgctg cccgcggccc tggccgggca gggcgttgcc atgcgctgcc tcgtgccagg
     1321 ctatcgtccg gtgatgtcgg cgctcggcgg catcgcggta ctgcaccgct atgccgacct
     1381 cttcggcgga ccggccagtc tggtcgcggg caaggccggc ggactcgatc tcatcgccat
     1441 cgatgcgccg cacctctatg accggcctgg caatccctat ctcgggccgg acggcaagga
     1501 ctggccggac aatgcccagc gtttcgcggc gctgtcccgg gcagcagccg aagtggcgcg
     1561 cggcatcatt cccggctatc agcccgatgt cctgcacctg cacgattggc aagcagctct
     1621 cgcttcagtc tatgtgaagt tccttggcgg accgaagacg ctgctcaccg tccacaacat
     1681 cgccttccag ggacagttcc ccgcctcgat cttcgcgtcg cttcagcttc ccgccgccgc
     1741 cttcgccatc gatggcgtcg agtactacgg cagcgtgagc ttcctcaagg gcggtctggc
     1801 aaccgccaat gccatctcga ctgttagccc ctcctacgcc caggagatct gcacgcccga
     1861 ctatggcatg ggtctcgatg gcctgctgcg ggcgcgccac gcggtgttga gcggcatcgt
     1921 caacggtatc gatgccgcgg tctggaaccc ggccgatgac cggggcattg ccgccaccta
     1981 cacggccaag accctgaatc gccgccgcgc caacaaacgc gaagtggaaa ggcgcttcgg
     2041 gcttgaggaa ggcgacggaa ttctccacgc agtggtgagc cgcctcacct ggcagaaggg
     2101 catggacatc ttcgtcgagt cgctggacga agtggtggca tcgggcgcgc ggctcgccct
     2161 catcggaaca ggcgaggcgc ctctggaggc cagtattcag gctgcaagcc agcgccacaa
     2221 gggccgcatc gg
//
LOCUS       DBNGVR010000119         2171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_41469, whole genome shotgun sequence.
ACCESSION   DBNGVR010000119 DBNGVR010000000
VERSION     DBNGVR010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_41469"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="AC16A0_02590"
     CDS             complement(<1..835)
                     /locus_tag="AC16A0_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3104248.1"
                     /translation="MDRILIFLLDSSNAILVLMLVSLGLAVIFGLMNVINMAHGEFLV
                     LGAYGVLTLQWAGLPFAACLVIAPMLVALIGLAAEELLIRRTYERLLDTILATWGLSL
                     VLRQGLVLIYGPGSHAVAAPDMGGVPVFGEIYPVYRLVVMALSAAAIAFTFWVFFRTA
                     LGLQARAVIANRNMAACLGINTRMLDRLTFAFGAGLAGLAGAVMSPLISVDPNAGLGW
                     LVPGFLSILVAGLGSLSGPIVGAAGVGAVDSLAAAISSPVWAQLTVFVAAIIVIRFFP
                     QG"
     gene            complement(836..1516)
                     /locus_tag="AC16A0_02595"
     CDS             complement(836..1516)
                     /locus_tag="AC16A0_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104249.1"
                     /translation="MLEIQELHAGYGGGEVLRGLSLGVGRGEIVALLGRNGMGKTTLL
                     RCVMGLLPHTAGSIRLEGLPLSGLDCFEIARRGVAYVPQGREIFGQLTVEENLRLAAL
                     RGGAIETGYELFPGLRARARDPGGSLSGGQQQQLAIARAMVAKPRLLLLDEPSEGIQP
                     SVVNEIADIVTRSARSMGLSVLLVEQNTDLALRVADRTVFVDHGVDAASIASSAVTRE
                     ILASYMEL"
     gene            complement(1509..>2171)
                     /locus_tag="AC16A0_02600"
     CDS             complement(1509..>2171)
                     /locus_tag="AC16A0_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104250.1"
                     /translation="KTFGGVTAADNISLSLESGEMLAIIGPNGCGKTTLFNLITGYFQ
                     PESGRVVFEGKDIAKLALYEVARLGIVRKFQVPSLFAGLTVEENLRIAGAAEPSDLLS
                     RFDLGAVRSQNAGTLAHGQKQWLELAMTLAARPRLLLLDEPAAGLTAAERGKTIDLVK
                     GLRKDFGLGIMLIEHDMRFVEALECPVSVMDMGRIIAAGTFAEVRRDSRVREAYLGVA
                     GA"
BASE COUNT          465 a          716 c          675 g          315 t
ORIGIN      
        1 gtccctgcgg gaagaatcgg atgacgatga tagccgcgac gaagaccgtc agctgtgccc
       61 agaccggcga cgagatggcc gcggccaggc tatcgaccgc accgacgccg gccgcaccga
      121 cgatgggacc ggagagggat ccgagtcccg ccacgaggat cgacaggaag ccgggcacga
      181 gccagcccag tccggcattg gggtcgaccg agatcagcgg cgacatcacc gctccggcca
      241 gtcccgcgag ccccgcgccg aaggcgaagg tcagccggtc gagcatccgg gtattgatgc
      301 cgaggcaggc cgccatgttg cggttggcga tgaccgcccg cgcctgcagg ccaagagccg
      361 tccggaagaa gacccagaac gtgaaggcaa tggcggcggc gctgagggcc atgacgacca
      421 ggcgatagac cggatagatt tcgccgaaga ccggaacgcc gcccatgtcc ggcgcggcca
      481 cggcatgcga gccgggcccg tagatcaaca ccaggccctg gcgcaagacc agggagagtc
      541 cccaggtcgc aaggatcgta tcgagcaggc gctcataggt tcggcggatg agaagctcct
      601 ccgcggcaag gccaatcagc gcaaccaaca tgggagcaat caccagacag gcggcgaagg
      661 gaaggccagc ccactgcaag gtgagcacgc cataggcgcc caggacgagg aattcgccgt
      721 gagccatgtt gatcacgttc atcagcccaa agatgaccgc gagcccgagc gataccagca
      781 tcagaaccag gatggcgttg gaggaatcga gaaggaagat caggatgcgg tccacctaaa
      841 gctccatgta tgacgccagg atctcgcgcg tgacggcact cgacgcgatg gacgctgcat
      901 cgacgccatg atcgacaaag acggtacgat ccgccacacg cagggcgagg tcggtgttct
      961 gctcgaccag cagcacggac aggcccatgg atcgggccga acgcgttaca atgtccgcga
     1021 tctcgttcac cacagacggc tgtatgcctt cggatggctc atcgagcaga agaagccgcg
     1081 gcttcgcaac catcgcgcgg gcgatcgcaa gctgctgttg ctgcccgccc gaaaggctgc
     1141 caccgggatc cctggccctg gccctgagac ccggaaagag ttcgtaacca gtctcgatcg
     1201 ctccgccgcg caacgccgcg agcctgaggt tctcttccac cgtcaactgc ccaaaaatct
     1261 cgcggccctg aggaacatag gcaacgcctc gccgcgcgat ctcgaagcag tcgaggcccg
     1321 acaggggcag tccctcgagc ctgatgctgc cggcggtgtg tggcaacaac cccatgacgc
     1381 agcgcaggag cgtggtcttg cccatgccgt tgcggccaag aagcgccacg atctcgccgc
     1441 gccccacgcc aagcgaaagc ccgcgcagca cctcgccacc gccgtagccg gcgtgcagct
     1501 cttgaatctc aagcaccggc gactccaaga taggcctcac ggacacgcga gtcccggcgg
     1561 acttctgcaa aagtgccggc ggcaatgatg cggcccatgt ccatcaccga caccgggcat
     1621 tcaagcgctt cgacaaagcg catgtcatgc tcgatgagca tgatgcccag gccaaagtcc
     1681 ttgcgcaggc ccttcaccaa atcaatggtc ttcccgcgtt cggcggccgt gagtccggca
     1741 gcaggttcgt cgagaagcag gaggcgcggc cgggccgcca gcgtcattgc cagttccagc
     1801 cattgcttct gaccatgagc aagcgttccg gcgttctggc tgcgcaccgc tcccaggtcg
     1861 aagcgcgaca gcagatcgga tggttcggcc gcaccggcaa tccgcaggtt ttcttcgact
     1921 gtcagtccag cgaagagcga tggaacctgg aatttccgga cgatgcccaa ccttgccacc
     1981 tcgtagagcg ccagcttggc aatgtccttg ccctcgaaaa ccacgcgccc cgactcgggc
     2041 tggaaataac ccgtgatcag attgaacagc gtggtcttgc cgcagccgtt cggcccgatg
     2101 atggccagca tctcgccgct ctcaaggcta agcgagatgt tatccgccgc agtgaccccg
     2161 ccaaatgtct t
//
LOCUS       DBNGVR010000120         9464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1612532, whole genome shotgun sequence.
ACCESSION   DBNGVR010000120 DBNGVR010000000
VERSION     DBNGVR010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1612532"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..638
                     /gene="rdgB"
                     /locus_tag="AC16A0_02605"
     CDS             33..638
                     /gene="rdgB"
                     /locus_tag="AC16A0_02605"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR3104251.1"
                     /translation="MRKLSGERIVIATHNKGKLAEFADLLKPYGVTAVSAGELGLAEP
                     AETGTTFAQNARIKAMAAMQATGMIAVSDDSGLCVAALDGAPGVYTADWAGPDRDWSR
                     AMRLVEEKLQASGAVTPERRRAEFVCTLCVIWPDGMERQFEGRVAGHLTWPPRGQLGH
                     GYDPMFVPDGGNRTFAELDPAEKNRISHRARALEALSVELL"
     gene            625..1773
                     /gene="hemW"
                     /locus_tag="AC16A0_02610"
     CDS             625..1773
                     /gene="hemW"
                     /locus_tag="AC16A0_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR3104252.1"
                     /translation="MSSSEAFGVYVHWPFCKAKCPYCDFNSHVRHAAVDAMSFARSLA
                     TELAWFAARSRGRQVTSIFFGGGTPSLMPPAAVGHVLDAIGRLWSVAPDVETTMEANP
                     TSIEAENFRGYRAAGVNRVSVGVQALNDADLKALGRQHTAAEALAAFRLAARVFPRVS
                     FDLIYARPEQTLEQWRKELRIALGEQQGHMSLYQLTIEPGTAYFDLHAKGSLMVPADD
                     RAADLYDVTQELTEAAGLPAYEVSNHAAAGQESRHNLLYWRYGEYAGAGPGAHSRIDS
                     GGRRLALVAERHPETWRDLVERQGHGIVTEAEVPPEDQASEYLLMGLRIAEGIDLTRY
                     AALAGREIDSSKIAGLKSLGLIKREGTRLRATPDGRKLLNALIAELAV"
     gene            complement(1770..1913)
                     /locus_tag="AC16A0_02615"
     CDS             complement(1770..1913)
                     /locus_tag="AC16A0_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104253.1"
                     /translation="MLARLGRGREWRVALATALCLMLAGCGGGKIGDLLKDTPAEEQQ
                     QQP"
     gene            1957..2862
                     /gene="rsmI"
                     /locus_tag="AC16A0_02620"
     CDS             1957..2862
                     /gene="rsmI"
                     /locus_tag="AC16A0_02620"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3104254.1"
                     /translation="MTKEPPQRHRRYSIGASGFEADSLGPGLYIVATPIGNLGDMTLR
                     GLATLAAADTVLCEDTRITARLLERYAIRVPLSPYHEHNAAKVRPGILARLKDGACIA
                     LVSDAGMPLVSDPGYRLVREAIAEGIPVTACPGPSAVLMGLALSGLPSDRFAFFGFMP
                     QKSGERKKLLGEIAALRATLVFFDSPHRICDTLADVAAALPGRQVAVARELTKLHEEV
                     LRGSAQEVADQLAQRAAVKGEITLLLGPPEAAAEAPREEDLAAAIAEALKTHSASRAA
                     AEIARRHGLSKKEIYDRILALKPDA"
     gene            2855..3223
                     /locus_tag="AC16A0_02625"
     CDS             2855..3223
                     /locus_tag="AC16A0_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3104255.1"
                     /translation="MRDGRREKAERAGRLAETAAMIFLSLKGYRLLSRRYKCPMGEVD
                     LIMRKGDTTAFIEVKIRNSIDAAIASVTAYQARRISAAARSYMGRDQIAARQACRFDI
                     VAVSPYHWPQHIENAFYGDG"
     gene            3226..4164
                     /gene="gshB"
                     /locus_tag="AC16A0_02630"
     CDS             3226..4164
                     /gene="gshB"
                     /locus_tag="AC16A0_02630"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR3104256.1"
                     /translation="MTLKVAVQMDPIDSINIKGDSTFAMMLEAQVRGHSLFYYQTRSL
                     ALGGGSLFATGHDIAVRDEAGNHFTLGESRRVDLGHYDVVLMRQDPPFDMGYITATHL
                     LDHIHPRTLVVNDPAEVRNAPEKLYVLRYPQFMPDTLITRDPAEIAAFRRTHGDIILK
                     PLYGNGGAGVFRLGEEDQNFSSLIELFTQAFREPFIAQQYLPDVRKGDKRIILVDGKV
                     AGAINRVPAANESRSNMHVGGRPEPTTLTKREHEICEALGPDLKARGLIFVGIDVIGD
                     CLTEINVTSPTGIREVQRFGGANIAALIWDAIESRR"
     gene            4339..5622
                     /locus_tag="AC16A0_02635"
     CDS             4339..5622
                     /locus_tag="AC16A0_02635"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3104257.1"
                     /translation="MAHIVVLGAGLGGTIMAYEMKEQLGRGDTLTVITKEPTYHFVPS
                     NPWVAVNWRKQEDIEVDLAPIFAKKGINFKAVAARKLDPEARRIDLEDGSSVDYDYLI
                     IATGPELAFDEIEGLGPQGHTQSVCHVEHAKVAGKAFEDFCKNPAPVVIGAVQGASCF
                     GPAYEFMFIVETELRRRKIRDRVPMTFVTAEPYIGHLGLDGVGDTKGLLESEMRNHHI
                     KWMTSSRVKKVEAGVMTVEEINEDGSVKATKELPFGFSMMLPPFRGVAAIRGIDGLTN
                     PRGFVIVDKHQRNPKYPEIFAIGVCVAIPPMGPTPVPCGVPKTGFMIESMVTATAMNI
                     GLLLKGKQPEHQGTWNAVCLADFGDSGVAFVAQPQIPPRNVNWSSSGKWVHGAKIAFE
                     KYFLHKIRRGTSETFYEKLALDVLGIGKLKETTRS"
     gene            5663..6085
                     /locus_tag="AC16A0_02640"
     CDS             5663..6085
                     /locus_tag="AC16A0_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3104258.1"
                     /translation="MTRSDLKRAVLLGTAMMSLAGPATADTAKQDFQSLCAPCHGADG
                     RGGGPAAAALRTRPADLTRIASRHGTFPVEKITEAVIGLDMPESHGSREMPIWGDRLI
                     NEALGEETSVAAAKSAAHDVEDRIAALVRYLETIQVKD"
     gene            complement(6082..7185)
                     /locus_tag="AC16A0_02645"
     CDS             complement(6082..7185)
                     /locus_tag="AC16A0_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3104259.1"
                     /translation="MGWSLTIGQIAGTSIRLHFTFLLLLAWIGIADYLAGGAAAAAAS
                     LVFILLIFACVTAHEFGHIFMARHFGVKTPEVILSPIGGMANMERIPEAPRQELLIAL
                     AGPAVNVVILLLLVIFAGVSLASLPGLDFVAADLASRLAYINGMLVLFNLIPAFPMDG
                     GRVLRALLSMAMGPDRATQLAARIGQSFAFLFVLLGLFYSPILLLVGIFVYFAATSEE
                     QASSLRWMARGLTVADAMERKPRLLRADEPLARAVDALLETPQRDFPVVDAAGLPVGL
                     LDREAMIAALAKHGRDAPISGAMRQAIVLHEGQPLEQAIDRMRLAGSKAEIVAAADGR
                     VIGLLSIENVAEMMMIRGAQPDWPFAAPAGRRS"
     gene            complement(7226..8116)
                     /locus_tag="AC16A0_02650"
     CDS             complement(7226..8116)
                     /locus_tag="AC16A0_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104260.1"
                     /translation="MTPDLKTPARSHVLPALAVSVCGLLWGVFWLPLRWFDSQGVGSA
                     WVALLFNLVAIAAPLPWLLKRPAWTDFPNQSLTGLILGTAFALYTVSLAMTDVIHAIL
                     LFYLTPVWSTLAGWVLLGERITATRIIAVILGFAGLWAILGGGGVPLPRNAGDWLALL
                     SGLLWSAGTLRSFARPSQGIALPVFCFAGGGLAASAVLLVIASALSLPMAAVSNLMPV
                     LPWIVLLAVIIFVPPNFLVLWAAQRMDPGRVGILLMTEVLVGSISAGLLAGEAYGASE
                     AIGTALIVTAGLVEVWGRRS"
     gene            8275..8517
                     /locus_tag="AC16A0_02655"
     CDS             8275..8517
                     /locus_tag="AC16A0_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104261.1"
                     /translation="MSALSHLKHGLIVAGILFPTAAFAGHWDLPSCYIDVHSGCFVNQ
                     SQPCTDAEYKDFLRMCHDTYPASKGVSTKKKFKVQG"
     gene            8716..>9464
                     /locus_tag="AC16A0_02660"
     CDS             8716..>9464
                     /locus_tag="AC16A0_02660"
                     /inference="COORDINATES: protein motif:HMM:NF021391.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HIR3104262.1"
                     /translation="MSSSGLAVLLATVTPASAQDSEAKAAFKAMSDFLSQQAALSVSY
                     DATLEIVTADLEKVGLASSGALNATRPDKLRATRSGGLANVELVFDGTEMSVYGKNLN
                     VFAKQAFKGTIDELVDALRLEFGLELPAADLLSANPHDIMMLNVTDAQALGTGIIRGQ
                     TCDHLAFRTDDVDWQIWIAQGDKPFPCRFTITSKMTVMAPSYDITFSDWKSGAEVAKD
                     DYKLVTAAGTTEVDISKLGSLDEVQMPKTEGA"
BASE COUNT         1835 a         3074 c         3020 g         1535 t
ORIGIN      
        1 ctggtggccc tgcagcgcca ggcgattgcg tgatgcgcaa gctgagcggt gagcgaatcg
       61 tgatcgccac ccacaacaag ggcaagcttg ccgagttcgc ggacctgctg aagccctatg
      121 gcgtgacggc ggtctcggct ggcgaacttg gccttgccga gcccgcggag acgggcacga
      181 ccttcgccca gaatgcccgc atcaaggcca tggccgcgat gcaggcgacg ggaatgatcg
      241 cggtctccga cgattccgga ctctgcgtcg cggccctgga cggggcaccc ggcgtctaca
      301 cggccgactg ggcaggtccc gaccgggact ggagcagggc catgcgactg gtcgaggaga
      361 agctgcaggc cagcggcgcc gtgacgccgg agcggcgccg cgccgaattt gtctgcaccc
      421 tttgcgtcat ctggcccgat ggcatggaac gccagttcga gggccgtgtg gcgggccatc
      481 tcacctggcc gccgcgcggc cagcttggcc atggctatga cccgatgttc gtgcccgatg
      541 gcggcaacag gaccttcgcc gaactcgatc cggccgagaa gaaccgcatc tcgcaccggg
      601 cccgggcact ggaggcgctg tccgttgagc tcctctgagg ccttcggagt ctatgtccac
      661 tggcccttct gcaaggccaa gtgtccttat tgcgatttca actcgcatgt gcggcacgcg
      721 gcggtggatg ccatgtcctt tgcccggagc ctggcaaccg aactggcatg gttcgccgcg
      781 cgaagcaggg gacgccaggt caccagcatc ttcttcggcg gtggcacgcc atcgctgatg
      841 ccgccggcgg ccgttggcca tgtgctcgat gcgatcggca ggctgtggag cgtggcaccg
      901 gacgtcgaga cgacgatgga ggccaatccc acctcgatcg aggctgaaaa cttccgcggc
      961 taccgggctg ccggtgtaaa ccgcgtctcc gtcggcgtgc aggcgttgaa tgatgccgac
     1021 ctcaaggccc ttggacggca gcacacggcc gcggaggcgc tggcagcttt caggctcgcg
     1081 gccagggttt tcccgcgcgt ctccttcgac ctgatctatg cgcggcccga acagacgctg
     1141 gagcaatggc gcaaggaatt gcgcatcgcg cttggcgagc agcagggcca catgtcgctc
     1201 taccaactca ccatcgaacc gggcacggcc tatttcgatc ttcatgccaa aggcagcctc
     1261 atggtgcccg cggatgaccg cgctgccgat ctctacgatg tgacccagga actgacagaa
     1321 gccgccggcc tgccggccta tgaagtctcg aaccatgccg cagccggaca ggagtcgcgg
     1381 cacaatctgc tctactggcg ctatggcgaa tatgccggcg caggccccgg cgcgcacagc
     1441 cgcatcgaca gtggcggccg gcgcctggcc ctggtggcag agcgccaccc ggaaacctgg
     1501 cgcgatctgg tggagcgcca aggtcatggg atcgtgacag aggcagaagt tccaccggaa
     1561 gatcaggcct cggaatatct gctgatgggc ctgcgcatcg ccgaaggcat cgatctgacc
     1621 cgctacgcgg ctctggccgg gcgcgaaatc gattccagca agattgccgg gctcaagtcc
     1681 ttggggctga tcaaacgtga gggcacacgg ttgcgagcaa cgcccgatgg ccggaagctg
     1741 ctcaatgcgc tgattgccga actggcagtc tagggttgct gctgctgttc ctcggccggc
     1801 gtgtccttga gcagatcgcc gatcttgccg ccgccgcagc cggccagcat caggcacaac
     1861 gccgttgcca gcgcaactcg ccactcccgt ccgcgcccaa gccgcgctag catcacatcc
     1921 gccattccac gtcccacctg acggttccgg agaaccatga caaaagagcc cccgcaaaga
     1981 caccgtcgct acagcatcgg tgcttctggt ttcgaggccg actcgctcgg accggggcta
     2041 tacatcgtgg ccacccccat cggcaatctc ggcgacatga ccttgcgcgg cctggcaacg
     2101 cttgccgccg ccgacaccgt gctgtgcgag gacacgcgca tcacggcgcg gctgcttgaa
     2161 cgctatgcca tccgggtgcc gctgtcgccc tatcacgagc acaatgcggc aaaggtgagg
     2221 cccgggattc tggcccggct caaggacggc gcctgcatcg ccctggtcag cgatgccggc
     2281 atgccgctgg tctccgatcc gggataccgc ctcgtccgcg aggccattgc ggaaggcatc
     2341 ccggtgaccg cctgtcccgg accttcggcc gttctgatgg ggctggccct gtcgggattg
     2401 cccagcgacc gcttcgcctt cttcggcttc atgccgcaga aatcgggcga gcggaaaaag
     2461 ctcctcggtg aaattgccgc gctgagggcc acgctggtct ttttcgacag cccccaccgc
     2521 atctgcgaca cgctggccga cgtggccgcc gccttgccgg gccgccaggt cgccgtggca
     2581 cgcgaactga cgaagcttca tgaggaagtg ctgcgcggat cggcgcaaga ggtggccgat
     2641 caactggcgc agcgcgcggc cgtcaagggc gagatcacgc tgctgctggg gccgcctgaa
     2701 gccgcggccg aggcgccgcg cgaagaagat ctcgcggcgg ccatcgccga ggccctgaag
     2761 acgcactccg cgtccagggc ggcagccgag attgcccggc gacacggcct ttcgaagaag
     2821 gagatctacg accgcatcct ggcgctcaag cccgatgcgt gatggacgcc gcgaaaaggc
     2881 cgagagggcc gggcgtctcg ctgaaacagc agcgatgatc tttctgagcc tcaagggcta
     2941 tcgtctgctg tcgcggcgct acaaatgccc gatgggcgaa gtcgatctca tcatgcgcaa
     3001 gggggacacc accgccttca tcgaggtgaa gatcaggaac agcatcgacg cggccatcgc
     3061 gtcggtgaca gcctaccagg cccgccgcat ctccgctgcc gcccgcagct acatgggccg
     3121 cgaccagatc gcggcgcggc aggcctgccg ttttgacatc gtggccgtca gtccctacca
     3181 ctggccgcag catatcgaga acgcattcta cggagacggc tgacgatgac gctcaaggtc
     3241 gcggtccaga tggaccccat cgattcgatc aacatcaagg gcgattccac cttcgccatg
     3301 atgctggagg cgcaggtgcg cggtcacagc ctcttctact accagacgcg ttcgctggcg
     3361 ctgggcggtg gcagcctctt cgccaccggc cacgacatcg cggtgcgcga cgaagccggc
     3421 aatcatttca ccctcggcga aagccgccgc gtcgacctgg gccactacga tgtcgtactg
     3481 atgcgccagg atccgccctt cgacatgggc tacatcaccg ccacgcatct gctcgatcac
     3541 attcatccga ggactttggt tgtgaatgat ccggccgaag tgcgcaacgc gccggagaag
     3601 ctctacgtct tgcggtatcc gcagttcatg ccggacacgc tgatcacccg cgacccggcc
     3661 gagatcgccg ctttccgccg cacccatggt gacatcatcc tgaagccgct ctacggcaat
     3721 ggcggcgcgg gcgtgttccg cctgggggag gaggatcaga acttctcatc gctcatcgag
     3781 ctcttcaccc aggccttccg cgagcccttc atcgcgcagc agtatctccc ggatgtccgc
     3841 aagggcgaca agcgcatcat tctcgtcgac ggcaaggtcg ccggcgccat caaccgggtg
     3901 cccgccgcca atgaaagccg ctccaacatg catgtgggcg ggcgtcccga gccgaccacg
     3961 ctgacgaagc gcgagcatga gatctgcgaa gcgctggggc ccgatctcaa ggcccgcggc
     4021 ctgatcttcg tggggatcga tgtgatcggg gactgcctca cggagatcaa cgtcacgtcg
     4081 ccgaccggca tccgggaggt gcaacggttt ggcggtgcca acattgcggc actgatctgg
     4141 gacgccatag aatcgcgcag ataatctata aatatcaata acttatcagt ctttcaagtg
     4201 cctttgcgcg gcggcgcgga gcgcggctgc aatcccactg gactccctgc caaggtcgta
     4261 tataataata tacgcatata ctaattataa tagatggcag gcctgataga cgggctcgac
     4321 aatgtggagg atttcaacat ggcgcatatc gtcgttctgg gtgccgggct tggcggcacg
     4381 atcatggcct acgagatgaa ggaacagctt ggccgcggcg acacgctcac ggtcatcacc
     4441 aaggagccga cctatcactt cgtgccatcc aatccctggg tggcggtgaa ctggcgcaag
     4501 caggaggaca tcgaagtcga ccttgcgccg atcttcgcca agaagggaat caacttcaaa
     4561 gccgttgctg cccgcaagct cgaccctgag gcaaggcgga tcgatcttga ggacggcagc
     4621 agtgtcgact acgactatct gatcattgca accggaccgg agcttgcctt cgacgagatc
     4681 gaaggactgg gaccgcaagg ccacacgcag tccgtctgcc atgtcgagca tgccaaggtc
     4741 gccggcaagg ctttcgagga cttctgcaag aacccggcac ctgtcgtcat cggcgcggtg
     4801 cagggggctt cgtgcttcgg cccggcctat gagttcatgt tcatcgtcga aaccgaactg
     4861 cgccgccgca agatccgcga ccgcgtgcca atgaccttcg tcaccgccga accctacatc
     4921 ggccacctcg gactcgatgg cgtgggcgac accaagggcc ttctcgagag cgaaatgcgt
     4981 aaccaccaca tcaagtggat gaccagcagc cgcgtcaaga aggtcgaggc cggcgtgatg
     5041 acggtggaag agatcaacga ggacggcagc gtcaaggcca cgaaggagct gcccttcggc
     5101 ttctccatga tgctgccgcc gttccgcggc gtggccgcga tccgcggcat cgatgggctc
     5161 accaatccgc gcggtttcgt catcgtcgac aagcaccagc gcaacccgaa gtatcccgag
     5221 atcttcgcga tcggcgtctg cgtcgcgatc ccgcccatgg ggccgacgcc ggttccctgc
     5281 ggcgtgccca agaccggctt catgatcgag tccatggtca cggcaacggc catgaacatc
     5341 ggcctgctgc tcaagggcaa gcagcccgag caccagggca cctggaacgc ggtctgtctt
     5401 gccgacttcg gcgactcggg cgtggccttc gtggcccagc cgcagatccc gccgcgcaac
     5461 gtgaactggt cgtcctccgg caaatgggtg catggcgcca agatcgcctt cgagaagtac
     5521 ttcctgcaca agatccggcg cggcaccagc gagaccttct acgagaagct ggcgctcgat
     5581 gtgctcggca tcggcaagct gaaggaaacc acccggtcct gaccgcttgc cagacgttcc
     5641 gggttggcgc aaaggcgagg acatgacgcg ctccgacctc aaacgggccg tgctgcttgg
     5701 cacggccatg atgtccctgg caggaccggc gacagccgat accgccaagc aggacttcca
     5761 gtccctctgt gcgccctgtc acggtgcgga cggccgcggc ggagggcctg cggctgccgc
     5821 cctcaggacg cgccccgctg acctcacccg gatcgcatcc cgccatggca cgtttccggt
     5881 cgagaagatc acagaggccg tcatcggact cgacatgccc gagtctcatg gatcgcgcga
     5941 gatgccgatc tggggcgacc gtctcatcaa cgaggcattg ggtgaggaaa cgtcggtggc
     6001 tgcagccaag tcggcggccc atgatgtcga agaccggatc gcggcgctgg tgcgctacct
     6061 cgaaacgatc caggtgaagg actagctgcg ccttccggcc ggcgccgcaa agggccagtc
     6121 cggctgggcg ccccggatca tcatcatttc agcgacgttc tcgatgctga gcaggccaat
     6181 gacgcgtccg tcggctgccg ccacgatctc ggccttgcta cccgcaaggc gcatcctgtc
     6241 gatcgcctgt tcgagcggct ggccttcgtg gaggacgatg gcctgacgca tggcgcccga
     6301 aatgggtgca tcgcggccat gcttcgccag ggcggcaatc atggcctccc ggtcgagcag
     6361 gccgaccggc aatcctgccg catccaccac ggggaagtcg cgctgcggcg tttccagcaa
     6421 tgcatcgacg gcacgtgcca gcggctcgtc cgcgcgcagc aggcgtggct tgcgttccat
     6481 cgcgtccgct accgtgagtc cacgcgccat ccagcgcaac gacgacgcct gctcctccga
     6541 tgtggcggcg aaatagacga agattcccac cagcagaagg atcgggctgt agaagaggcc
     6601 gagcagcacg aagaggaacg caaagctctg cccgatgcgg gcggccagct gggtggcgcg
     6661 gtcgggaccc atggccatgg agagcagtgc ccgcagcacc cggccgccat ccatcggaaa
     6721 ggccgggatc agattgaaca ggaccagcat gccattgatg taggcgagcc ggctggcaag
     6781 atcggccgcg acgaaatcga gaccgggcag cgatgcaaga ctgacgccgg cgaagatcac
     6841 gagcagcaga aggatgacga cattgaccgc ggggccagcc agggcgatca gcagttcctg
     6901 tcgcggcgct tcggggatgc gctccatgtt cgccatgccg ccgatcggcg agagaatgac
     6961 ctccggcgtc ttcaccccga agtgacgggc catgaagata tggccgaatt catgtgcggt
     7021 gacgcaggca aagatcagca ggataaagac cagcgaggcg gcggcggccg ccgctccgcc
     7081 ggcgaggtag tcggcgatgc cgatccaggc cagcagcagc aggaaggtga agtgcagccg
     7141 gatcgaggtc ccggcgatct gtccgatggt aagtgaccag cccatgatgt tggcagtcta
     7201 gcagcttgcc gccgaagccg gaacctcagc ttctgcggcc ccacacctcg accagacccg
     7261 cagtgacgat cagcgcggta ccgatggcct cgctggctcc ataggcttcg ccggcaagca
     7321 ggccggcgga aatcgaaccg acgagcactt ccgtcatgag cagaatgccg acgcggcccg
     7381 gatccatgcg ctgggcggcc cagagcacca ggaaattggg cggcacgaaa atgatgacgg
     7441 cgaggaggac gatccagggc aggaccggca tgaggttgct gacggcggcc atgggcaggg
     7501 acagcgccga tgcgatgacc agcagaaccg ccgaggcggc aagtccgccg ccagcgaagc
     7561 agaagaccgg aagtgcaatg ccctgcgacg gccgcgcaaa gctgcgcagg gttccggccg
     7621 accacagcag gccgctgaga agcgccagcc agtcgccggc gttgcgcggc agcggaacgc
     7681 cgccgcctcc gagaatcgcc cagagcccgg cgaagcccag aatgactgcg atgatccgcg
     7741 tcgcggtaat gcgttcgcca agcaggaccc agcctgccag cgtgctccag acgggcgtga
     7801 gatagaacag caggatggca tggatgacgt cggtcatcgc caggctgacc gtgtagagtg
     7861 cgaaggccgt tccgaggatc aggcccgtga gcgactggtt ggggaaatcg gtccaggctg
     7921 gtctcttgag cagccagggc agcggcgctg caatggcgac cagattgaac agcagcgcga
     7981 cccaggcact gcccacgccc tggctgtcga accagcgcag cggcagccag aagactcccc
     8041 agagcaggcc gcagaccgac acggcgaggg ccggcagcac gtgggagcgc gcaggcgtct
     8101 tgaggtctgg ggtcatgcga tccttatgcg ggtcggccag gcgggccgca accggcgcac
     8161 cggcatggtt gccctgcatc tgcatgaacg ccgggtgaag cgccgtctca tcgagggttc
     8221 agggccgctc ccgcaaaaag gcatcactcg tcaaccccaa gaaaggatcg tacaatgtct
     8281 gctctatcgc acctcaagca tggcctcatc gttgccggca ttctgttccc gaccgccgca
     8341 ttcgccggcc actgggatct gccgtcctgc tacatcgacg tgcacagcgg ctgcttcgtc
     8401 aatcagtcgc agccctgcac cgacgccgag tacaaggact tcctccgcat gtgccacgac
     8461 acctacccgg ccagcaaggg tgtgagcacc aagaagaaat tcaaggttca gggctgaaag
     8521 gcaagggccg aagctcgacg ccaatgccga aaccacatgc gggccggccg cacgccttgc
     8581 tcatgcggcc ggcacgcgcg ccggttgaaa tcgatacagt ggaatctggg cccgactggc
     8641 gctaaacaat attctttgga tggaatcaca gaccttgaag ggggatgcag gttgttcaga
     8701 aacgcagcga gattcttgtc gtcgagcgga ctcgctgtcc tgctcgcaac cgtcacgccg
     8761 gcaagcgccc aggactcgga ggccaaggcg gccttcaagg ccatgtcgga tttcctgtca
     8821 cagcaggcgg ccttgtccgt cagctacgat gccacgctgg agatcgtgac cgcggacctt
     8881 gagaaggtcg gtcttgccag ctccggcgca ttgaatgcga cgcgacccga caagctgcgt
     8941 gctacccggt ccggcggcct cgccaatgtc gaactcgtct tcgacggcac cgaaatgtcg
     9001 gtctatggca agaatctcaa tgtcttcgcg aagcaggcct tcaagggcac gatcgacgag
     9061 ctggtcgatg cgctgcgtct cgagttcggg cttgagctgc cggcagccga tctgctctcg
     9121 gccaatcccc atgacatcat gatgttgaac gtgacggacg cccaggcgct cggcaccggc
     9181 atcattcgcg gacagacctg cgatcatctc gccttccgca ccgacgatgt cgactggcag
     9241 atctggatcg cacagggcga caaacccttt ccctgccgct tcaccattac cagcaagatg
     9301 acagtgatgg cgccgtccta cgacatcacg ttctccgatt ggaagagcgg tgcagaagtc
     9361 gccaaggacg actacaagct cgtcaccgct gcaggtacga cggaggtcga catcagcaag
     9421 ctggggtcgc tggatgaggt gcagatgccg aagaccgaag gagc
//
LOCUS       DBNGVR010000121         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_42025, whole genome shotgun sequence.
ACCESSION   DBNGVR010000121 DBNGVR010000000
VERSION     DBNGVR010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_42025"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2064
                     /locus_tag="AC16A0_02665"
     CDS             <1..2064
                     /locus_tag="AC16A0_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR3104263.1"
                     /translation="PHYNMENVAGGVFLPKDGQVNPIDVTQALAAGARSRGARILENT
                     KVTRILVKDGKAVGVETADGTIMADKVVVASGMWSRDLGRSIGVNIPLHACEHFYIVS
                     EPIADLPRNMPVVRVPDECTYYKEDAGKLMVGAFEPKAKPWGGGGIDEDHAFVTLPED
                     MDHFEPILANAINRVPLLETAGIQLFFNGPESFTPDVRYYLGEAPEVKDVYVATGFNS
                     IGIQSSGGAGLVLAKWIKNGHPPMDVNGIDIRRIHPFQSVRNYVRERVSESLGLLYAM
                     HWPYRQAETARGVRRSPLYPYTKQLGAVFGEVNGWERPNWYARDGVKPEYAYSYGRQN
                     WFPCADHEAKQLMSNCAFFDQASFAKYAVEGRAACKVLNRISANDVDVEPGRIVYTQW
                     LNERGGIEADLTVTRLAEDRFLVVTGAVPQTRDMAWLKRHTPDDASCIAIDITSGLPM
                     IAVMGPKSRALLEKLSGADLSNAAFPFGTSQEIEIGYARVCASRITYVGELGWELYVP
                     AEFAAHVFETLQTAGTEFGLTPAGMHTMNNCRMEKAYRHWGHDISDEETPIEAGLGFA
                     LAWDKPGGFIGREALLKQRDRKQQPKRMICLALDDNSATAPMIYHEEPIYRDGVIVGS
                     TTSGAWGHRVNLSLGLGYVRNKAGVTKEWIESGNWEVELAWKRHPARVQLQSFYDPKG
                     ERIKA"
BASE COUNT          435 a          738 c          645 g          372 t
ORIGIN      
        1 ccgcactaca acatggagaa tgtcgctggc ggcgtctttc tcccgaagga cggacaggtc
       61 aatccgatcg atgtgaccca ggccctcgcc gccggcgcac gctcacgtgg cgccaggatc
      121 ctggaaaaca ccaaggtcac ccgcatcctg gtcaaggatg gcaaggccgt gggcgtggaa
      181 accgccgacg gcaccatcat ggccgacaag gttgtcgtcg cttccggcat gtggtcgcgc
      241 gatctcggcc gctccatcgg cgtcaacatc ccgctgcatg cctgcgagca cttctacatc
      301 gtctcggaac cgatcgcaga cctgccgcgc aacatgccgg tggtgcgcgt gcccgacgaa
      361 tgcacctatt acaaggaaga tgcgggcaag ctcatggtcg gtgccttcga acccaaggcc
      421 aagccctggg gcggcggcgg catcgacgag gaccatgcct tcgtcacgct gccggaagac
      481 atggatcact tcgaaccgat cctcgcaaat gccatcaacc gcgtgcccct cctcgaaacg
      541 gcaggcatcc agctcttctt caatggcccg gagagcttca cgcccgatgt ccgctactat
      601 cttggcgagg cgcccgaggt gaaggacgtc tatgtcgcca ccggcttcaa ctccatcggc
      661 atccagtcgt cgggaggtgc gggcctggta ctcgccaagt ggatcaagaa cggccatccg
      721 cccatggacg tgaacggcat cgacatccgc cgcatccatc ccttccagtc ggtcaggaac
      781 tacgtgcgcg agcgcgtctc cgagagcctc ggcctgctct atgccatgca ctggccctat
      841 cgccaggccg agacggcgcg cggcgtcagg cgctcgccgc tttatccgta cacgaagcaa
      901 ctgggcgcag tcttcggcga ggtcaacggc tgggagcgcc ccaactggta cgcccgcgac
      961 ggcgtcaagc cggaatacgc gtacagctac ggccgccaga actggttccc ctgtgccgac
     1021 cacgaagcca agcagctgat gagcaactgt gccttcttcg accaggcgag cttcgccaag
     1081 tatgcggtcg aaggccgcgc ggcctgcaag gtgctcaacc gcatcagtgc caacgacgtg
     1141 gacgtcgagc ccggccgcat cgtctacacg caatggctca acgaacgcgg cggcatcgag
     1201 gcggatctca ccgtgacacg gcttgccgaa gaccgtttcc tggtcgtgac cggcgcggtg
     1261 ccgcagaccc gcgacatggc ctggctaaaa cgccacaccc cggacgatgc cagctgcatc
     1321 gccatcgaca tcacctccgg cctgcccatg atcgccgtca tgggaccgaa gtcacgcgcg
     1381 ttgcttgaaa agctgtctgg cgccgatctc tccaatgcgg ccttcccctt cggtacctcg
     1441 caggaaatcg agatcggcta tgcccgcgtc tgcgccagtc gcatcaccta tgtgggcgaa
     1501 ctgggctggg aactctacgt gccggccgag tttgccgccc atgtctttga aacgctgcag
     1561 accgcaggaa ccgagttcgg cctcaccccc gccggcatgc acaccatgaa caactgccgc
     1621 atggagaagg cctaccgcca ctggggccac gatatctccg acgaagagac gccgatcgag
     1681 gccggcctgg gctttgccct cgcctgggac aagcccggcg gatttatcgg ccgcgaggcg
     1741 ctgctgaagc aacgcgaccg caaacagcag cccaagcgca tgatctgcct cgcgctggac
     1801 gacaattccg ccacggcacc gatgatctat cacgaagagc cgatctaccg cgatggcgtg
     1861 atcgtgggtt ccaccacctc gggcgcctgg ggccaccggg tgaacctctc gctcggcctg
     1921 ggctacgtcc gcaataaagc tggggtcacg aaggaatgga tcgagtcagg caattgggaa
     1981 gtcgaacttg cctggaagcg ccacccggcc cgcgttcagt tgcagagctt ctatgatccg
     2041 aagggcgaac gcatcaaagc ctagctggcg gacgcagagg catgactgct gattgggcaa
     2101 gtcagatcgt gctgccattg tattcggcac actcatgccc aactccttcg caatctgccc
     2161 aaaaatcgga caggttcctt gactttcgtc
//
LOCUS       DBNGVR010000122         6625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2596558, whole genome shotgun sequence.
ACCESSION   DBNGVR010000122 DBNGVR010000000
VERSION     DBNGVR010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2596558"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="AC16A0_02670"
     CDS             <1..710
                     /locus_tag="AC16A0_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3104264.1"
                     /translation="DAAALERTLAQTGGDTVICDASYRDILRRWADQQDSHAPTRQVF
                     VMMRAEERRQLQDLLLQPFAGYLLKPFRRQTVLSRLAPASEAAIDAQIAGLRSMAETS
                     RKAAGLNVLLAEDNPVNALLARTMLQKAGCRVQHASNGLEVLASLDAGTRPDLVIMDV
                     EMPGLDGLATARRIRADEAAAGLPAIPILALTANARPEDIAECLEAGMNGHLSKPFDR
                     QDLDEAVARLVGNRSAA"
     gene            769..1596
                     /gene="olsB"
                     /locus_tag="AC16A0_02675"
     CDS             769..1596
                     /gene="olsB"
                     /locus_tag="AC16A0_02675"
                     /EC_number="2.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043810 - ornithine-acyl [acyl carrier
                     protein] N-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="l-ornithine N(alpha)-acyltransferase"
                     /protein_id="HIR3104265.1"
                     /translation="MPNLTASPDIKVERADLILRMARSAAEIDACQHLRYRVFYEELH
                     AKPAAEAAGSRRDADRFDAICDHLVVIRTTPGPAGDPIMLADGELVGTYRLLRQEVAE
                     AHGGFYTAGEFDIAPLLARHGTLRFLELGRSCVLKPYRTKPVVELLWQGIWNYVRAHR
                     LDVMIGCASLEGIDPDLHAATLSFLAQSAAAPDEWRVHALPDRSVEMNRVPAGSVSHK
                     EALRQLPPLIKGYLRLGCYIGEGAVIDRQFNTIDVLIVLPVSAINPRYFAHFGAPSG"
     gene            complement(1586..3865)
                     /locus_tag="AC16A0_02680"
     CDS             complement(1586..3865)
                     /locus_tag="AC16A0_02680"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR3104266.1"
                     /translation="MSTEKRPGRRPTITDEEALQFHQQGKPGKLEITPTKPMATQRDL
                     SLAYSPGVAVPVRKIAEDPATAYDYTAKGNLVAVISNGTAILGLGDLGALASKPVMEG
                     KAVLFKRFADVDSIDLEVDTRDVDQFVGAVRYLGPSFGGINLEDIKAPDCFIIEQRLR
                     ELMDIPVFHDDQHGTAIIATAGLINALHLTGRDLKSFKLVCNGAGAAAIACVELIKSL
                     GVPNENVILADSKGVVYKGRSDGMNQWKSAHAVATKARTLAEALEGADVFFGLSIAGA
                     LTPAMVRSMAPNPIIFAMANPDPEITPEEVAKVRSDAIMATGRSDYPNQVNNVLGFPY
                     IFRGALDVRARTINEEMKIAAAQALANLAREDVPDEVAAAYHGSRLSFGRDYLIPVPF
                     DPRLISTVPVAVAKAAMDSGVARRHIADLDAYVAELSARLDPIARTIQAVFEKVRISP
                     KRVVFAEGEEDRMIRAANSYAAAGLGRAILIGRESEIEKTLAVSGLELHENVDVLNAR
                     ISERNVDYANYLYERLQRNGFLFRDCQRMANQDRNIFAACMVALGDADALVTGLTRNF
                     SVALDNVRRAIDQRPGHRPIGVSMAMVRGRTVFIADTSIHELPSAEELADIAQEAAGV
                     ARRFGYEPRVALLSYSTFGYPRGERAQYVRDAVGVLDRRNVDFEYDGEMGADVALSRE
                     AMALYPFCRLTAPANVLVMPAAHSASISTKMLQQLGGVTVVGPLLTGLDKPVQVASMS
                     ATASDIVNLAAIAAYDINR"
     gene            3985..>6625
                     /gene="mutS"
                     /locus_tag="AC16A0_02685"
     CDS             3985..>6625
                     /gene="mutS"
                     /locus_tag="AC16A0_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018447781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3104267.1"
                     /translation="MNDGPATEASPDGAADQRATPVMAQYLEIKTANPGSLLFYRMGD
                     FYELFFHDAEIAARDLGIALTKRGRHQGADIPMCGVPVHAAEGYLQRLIRIGHRVAVC
                     EQLEDPAEARKRGSKSVVRRDVVRLVTPGTLTEDSLLDQRSHNYLAAIARLRGSGEAA
                     LAWADISSGELAVQPTTPGRLAADLARLGPSEILVPDSLLDEHPYRDILKAQAAPVTP
                     LPGVRFDSVSAEHRLRTHFGVAALEAFGSFSKAETAALGGLIEYITLTQVGRMPYLRP
                     PLRQEPAGFLLVDAATRANLELTRSLNGDRRGSLLAVIDRTVTASGARLLAERLSAPL
                     ADPDAVAARLDEIDFFLAAPSLRSTLQAALKAMPDIERALGRLSLGRGGPRDLAAIRD
                     GLSCAMALARSLSAHQGLGGLPQNIAASCAVLADADAALAAELGRALAEELPLLARDG
                     GLVRSGYLADLDANRELRDATRQVIAGLQTQYAEDSGIKSLKIRHNNVLGYFIEVTAQ
                     HAGVLQARPGTYIHRQTVASAMRFVTTELAELEQRIAAAGDRVLAMELEIFATLSARV
                     VDQRDRLAVIAGAIAAIDVASSLAELAETNRYVRPKVDSSKAFEIRAGRHPVVEAMLR
                     ERAESNFVANDCELSGDGRRLWLITGPNMAGKSTFLRQNALIVVLAQMGCFVPADFAH
                     VGVVDRLFSRVGAADDLARGRSTFMVEMVETAAILNQATDRSFVILDEIGRGTATFDG
                     LSIAWATIETLHDVTRCRALFATHYHELTALAGRLGQLANATVRVREWQGDVVFLHEV
                     VPGVADRSYGIQVARLAGLPQPVIERASEILERLEQDGQARAPKALIDDLPLFSASRS
                     QQRAAAPQGPSDIEKKLAALSPDE"
BASE COUNT         1099 a         2216 c         2196 g         1114 t
ORIGIN      
        1 gcgatgctgc ggctctcgaa aggaccctgg cgcagacggg tggcgatacc gtcatctgcg
       61 acgcttccta tcgcgacatc ctgcggcgct gggctgacca gcaggactcc catgcgccca
      121 cccgccaggt cttcgtgatg atgcgcgccg aagagcgccg ccagctgcag gatctgctgc
      181 tccagccttt cgcgggatat ctgctgaagc cgttccgccg tcagaccgtg ttgagccgcc
      241 tggcgcccgc ctcggaggct gcgatcgatg cacagatcgc cggactgcgg agcatggccg
      301 agacgtcgcg caaggcggca ggcctcaacg tgctcctcgc cgaggacaat ccagtcaatg
      361 cattgctggc gcgcaccatg ctgcagaagg ccggctgccg cgtgcagcac gcgagcaatg
      421 gcctcgaagt gctggcgtcc ctcgacgcgg ggacccgccc cgacctcgtc atcatggatg
      481 tcgagatgcc gggtctcgac ggactggcca cggcacggcg cattcgtgcg gacgaagctg
      541 cggcaggcct gccggcgata ccgatcctgg cgctcaccgc caatgcgcga cccgaagaca
      601 tcgccgaatg cctggaggcc ggcatgaacg gccatctctc gaagcccttc gaccgccagg
      661 acctggacga ggccgtcgcg cggctcgtcg gaaaccgcag cgccgcctga gagcgccgcc
      721 acgggactgt caaagtcccg cgctctatgg caagcacgtg gcgagggagt gccgaatctg
      781 accgccagtc cggatatcaa ggtggagcgc gccgacctga tcctgcgcat ggccaggagt
      841 gccgcggaga tcgacgcctg ccagcatctg cgctaccgcg ttttctatga ggagctgcat
      901 gcgaagcccg ctgcagaagc agcgggcagc cgtcgcgatg ccgaccggtt cgatgcgatc
      961 tgcgatcacc tggtggtgat caggacgaca cccgggccgg ccggcgatcc gatcatgctg
     1021 gccgatggcg agctggtcgg aacctaccgc ttgctgcgcc aggaagtggc cgaggcccac
     1081 ggcggcttct ataccgccgg cgagttcgac attgcgccac ttctggcgcg ccacggcaca
     1141 ctgcgcttcc tcgaactcgg ccggtcctgc gtgctcaagc cctatcgcac caaacccgtg
     1201 gtcgagctgc tgtggcaggg tatctggaac tatgtgcgcg cccatcgcct tgacgtgatg
     1261 atcggttgcg ccagcctcga gggaatcgat cccgatctcc atgccgcgac gctgagcttc
     1321 ctcgcgcagt ccgccgccgc gccggacgaa tggcgggtgc acgcgctgcc ggatcgatcc
     1381 gtcgagatga accgcgttcc cgcaggcagc gtgagccaca aggaggcgct gcgccagttg
     1441 ccgccgctga tcaagggcta tctccggctc ggctgctata tcggcgaggg cgccgtcatc
     1501 gaccgccagt tcaacaccat cgatgtgctg atcgtgcttc ctgtctcagc gatcaatcca
     1561 cgttatttcg cgcatttcgg cgcgcctagc ggttgatgtc gtaggcggcg atggccgcaa
     1621 ggttcacgat gtccgaggcg gtggccgaca tggaagccac ctgcacgggc ttgtcgaggc
     1681 ccgtgagcaa gggcccgacc acggtgacgc cgcccagctg ctgcagcatc ttggtggaga
     1741 tcgaggccga atgtgcggcc ggcatcacca ggacattggc tggcgctgtg agcctgcaga
     1801 agggatagag cgccatggcc tcgcgcgaca atgccacgtc ggctcccatc tcgccgtcat
     1861 actcgaagtc gacattgcgg cggtcgagca cgccgacggc gtcgcgcaca tattgcgcgc
     1921 gctcgccgcg cggatagccg aaggtggagt aggaaagcag cgccacccgc ggctcgtagc
     1981 cgaagcgccg ggcgacacct gccgcctcct gcgcgatgtc ggccagttcc tcggccgagg
     2041 gcagctcgtg aatggaggta tcggcgatga atacggtacg gccgcggacc atcgccatgg
     2101 agacgccgat ggggcggtgt ccgggacgct ggtcgatggc gcgccggaca ttgtcgagcg
     2161 cgaccgagaa attgcgcgtc aggccggtga ccagcgcatc ggcgtcgccc agcgccacca
     2221 tgcaggcggc gaagatgttg cggtcctggt tggccatgcg ctggcagtcg cggaacagga
     2281 agccgttccg ctgcaggcgc tcgtagagat agttggcgta gtccacgttg cgctcggaga
     2341 tgcgggcatt gaggacatcg acattctcgt gcagttcgag cccgctcaca gccagcgtct
     2401 tctcgatctc gctttcgcgc ccgatgagaa tggcccggcc aagtcccgcg gcggcatagc
     2461 tgttggcggc ccggatcatg cggtcttcct cgccctcggc aaagaccacc cgcttgggcg
     2521 aaatgcgaac cttctcgaaa acggcctgaa tggtgcgcgc gatggggtcg agccgcgccg
     2581 agagttccgc cacataggca tcgagatcgg cgatatggcg ccgtgcgacg ccgctgtcca
     2641 tcgcggcctt tgccacggcg accggcacgg tcgagatgag gcgcggatcg aagggcacgg
     2701 ggatgagata gtcccgcccg aagctgagcc gcgagccgtg ataggcggcg gccacctcgt
     2761 ccggcacgtc ctcgcgcgcc aggttggcca gggcctgggc cgcagcgatc ttcatctctt
     2821 cattgatggt gcgggcccgc acgtcgagcg cgccgcggaa gatgtaggga aagccaagga
     2881 cgttgttgac ctggttggga tagtcggagc gccccgtcgc catgatggca tccgatcgga
     2941 ccttggccac ttcctccggg gtgatctcgg gatcgggatt ggccatggcg aagatgatgg
     3001 gattgggcgc catgcttctg accattgcgg gggtcaaagc gccggcgatc gagaggccga
     3061 agaagacatc cgctccctcc agggcctcgg ccagggtccg cgccttggtg gcgaccgcat
     3121 gcgcggactt ccactgattc atgccgtcgc tgcggccctt gtagaccacg cccttggagt
     3181 cggcgaggat gacgttctcg ttgggcacgc ccagcgactt gatgagctcg acgcaggcga
     3241 tggcggcggc acctgcgccg ttgcagacca gcttgaagga cttgagatcg cggcccgtca
     3301 gatggagcgc attgatcaga cccgcggtgg cgatgatcgc ggtgccgtgc tggtcgtcgt
     3361 ggaagaccgg gatatccatg agttcgcgca ggcgctgctc gatgatgaag cagtcgggcg
     3421 ccttgatgtc ctccagattg atgccgccga aggacggtcc cagataacgc accgcaccga
     3481 cgaactggtc gacgtcccgg gtgtcgacct caaggtcgat cgaatcgacg tcggcgaagc
     3541 gcttgaagag caccgccttg ccttccatca ccggcttgga ggccagcgcg ccaaggtcgc
     3601 caagacccag gatggccgta ccgttggaaa tgacggccac caggtttccc ttggcggtgt
     3661 agtcataggc ggtggcggga tcctccgcga tcttgcggac tggtacggca acgccagggc
     3721 tataggccag cgacaggtcg cgctgggtgg ccatcggctt tgtgggcgtg atttccagtt
     3781 tgccaggctt gccctgctgg tggaactgca gcgcctcttc gtcggtgatc gtggggcgtc
     3841 ggcccggcct cttctcggta ctcatggatg acagacccca aagcgatgca atggcgcgag
     3901 actagggcgg cggcaccgca ttctcaagcc tcgtatcggc aagcgccttg tgagttttgc
     3961 cgattttcct gctaatggag gctgatgaac gacgggcccg ccaccgaggc cagtccggat
     4021 ggtgccgccg accagcgcgc cacgccggtc atggcgcagt acctggagat caagactgcc
     4081 aatcccggca gcctgctctt ctatcgcatg ggcgatttct acgagctctt cttccacgat
     4141 gccgagattg ccgcgcgtga cctgggcatc gcgctcacca agcgcggccg ccaccagggt
     4201 gccgacattc ccatgtgcgg cgtcccggtg catgcggcgg aaggttacct gcagcggctg
     4261 atccgcatcg gccaccgggt ggcggtctgc gaacagctgg aggatccggc cgaagcccgc
     4321 aagcgcgggt cgaaatccgt cgtacggcgc gatgtcgtcc ggctggtgac gcctggcacg
     4381 ctgaccgagg acagcctgct cgaccagcgc agccacaact acctcgccgc catcgcgcgg
     4441 ctgcgggggt cgggcgaggc ggccctcgcc tgggccgaca tctcctccgg cgagctggcg
     4501 gttcagccca ccacaccggg acggctggca gccgatctgg cccgtctcgg tcccagcgag
     4561 atcctggtgc ccgacagcct gctcgacgag catccctacc gcgacatcct caaggcccag
     4621 gcggcccccg tgaccccgct gccgggcgtg cgattcgatt cggtctcggc ggagcatcgc
     4681 ctcaggacgc acttcggcgt ggccgccctc gaggccttcg gcagtttttc caaggcggag
     4741 accgcagccc ttggcggact catcgagtat atcaccctca cccaggtggg gcgcatgccc
     4801 tatctgcgtc cgcccctccg gcaggaaccg gccggatttc ttctggtcga tgccgcaacg
     4861 cgcgccaatc tggaactcac ccgcagtctc aacggcgacc gccgcggcag ccttttggcc
     4921 gtcatcgacc gcaccgtcac ggcgagcggc gcgcggctcc tggccgaacg gctctcggcg
     4981 ccgctcgccg atccggacgc ggtggccgcg cggctcgacg agatcgactt cttcctcgcc
     5041 gcaccatcgc tgcgcagcac cttgcaggca gcgctcaagg cgatgcccga tatcgagcgg
     5101 gcccttggcc gcctgagcct cggccgcggc ggcccgcgcg atctggccgc gatccgcgac
     5161 ggcctgtcct gtgccatggc cttggcacgc agcctgtcgg cgcatcaggg gcttggcgga
     5221 ttgccgcaga acatcgctgc atcctgcgcc gtgcttgccg atgccgatgc tgcgcttgcc
     5281 gcagaactcg gtcgggcgct ggcggaagaa ctgcccttgc tcgcgcgcga tggcggcctc
     5341 gtccgctccg gctatctcgc cgatctcgat gccaatcgcg aactgcgcga tgccacccgc
     5401 caggtgattg ccggactgca gacgcaatat gccgaggaca gcggcatcaa gtcgctgaag
     5461 atccggcaca acaatgtcct cggttacttt atcgaggtca cggcgcaaca tgccggcgtg
     5521 ctgcaggcaa ggcccggcac ctacatccac cgccagacgg tggccagcgc catgcgcttc
     5581 gtcaccaccg aactggcgga actcgaacag cgcatcgcgg cggccggcga ccgcgtgctg
     5641 gcaatggaac tggagatctt cgccacgctt tcggcccgcg tggtcgacca gcgcgacaga
     5701 ctcgccgtca tcgcaggggc catcgccgcc atcgatgtcg cgtcgtcgct cgcagagctg
     5761 gccgaaacaa accgctatgt gaggccaaag gtcgattcct ccaaggcctt cgagatccgc
     5821 gccggacgcc acccggtggt cgaggccatg ctccgcgaac gcgccgagag caatttcgtc
     5881 gccaatgact gtgagctttc gggagatggc cgcaggctct ggctcatcac cggtcccaac
     5941 atggccggca agtccacctt cctgcggcag aacgcgctca tcgtggtgct ggcgcagatg
     6001 ggctgcttcg tgcctgccga ttttgcccac gtgggcgtcg tcgaccggct cttcagccgg
     6061 gttggagccg ccgacgatct ggcacgcggg cgttccacct tcatggtgga aatggtcgaa
     6121 acggcggcga tcctgaacca ggccaccgac cgttccttcg tcattctcga tgaaatcggc
     6181 cgcggaacgg caaccttcga cgggctttcc attgcctggg ccaccatcga aacgctgcat
     6241 gacgtcacgc gctgccgcgc gctcttcgcc acgcattatc acgaactgac cgcgctggcg
     6301 gggcggctcg gccagctggc gaacgccacg gtacgggtgc gcgaatggca gggcgacgtg
     6361 gtcttcctgc acgaggtcgt gcccggcgtg gccgaccggt cctacggcat ccaggtggcg
     6421 cgcctcgccg gcctgccgca gccggtgatc gagcgtgcca gcgaaatcct cgagcggctg
     6481 gaacaggacg ggcaggcccg ggcgccgaaa gccttgatcg atgaccttcc gctgttctcc
     6541 gccagccgca gccagcaacg tgcagcagcg ccacaggggc cgagcgacat cgaaaagaag
     6601 cttgccgccc tgtcgccgga tgagc
//
LOCUS       DBNGVR010000123         5768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_829915, whole genome shotgun sequence.
ACCESSION   DBNGVR010000123 DBNGVR010000000
VERSION     DBNGVR010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_829915"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..943
                     /locus_tag="AC16A0_02690"
     CDS             515..943
                     /locus_tag="AC16A0_02690"
                     /inference="COORDINATES: protein motif:HMM:NF023737.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104268.1"
                     /translation="MTSRRDMIRGLLAGILSAAGAAPVDHAAGETPKAADFIAFSERL
                     MEVKGLDPGMGADILGAFVDVGQGEALASLMTQTGPIDAGNTMANAVVAAWYSGLSTR
                     TAGEIVTDFNGALMWDALSFTKPWGSCGGATGYWGDPPSG"
     gene            962..2581
                     /locus_tag="AC16A0_02695"
     CDS             962..2581
                     /locus_tag="AC16A0_02695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3104269.1"
                     /translation="MADAISADVVIIGSGISGAMLSAGLAKAGVKVAILEAGDQVDRA
                     TAVNTYWNAAIKVPECAYPPTAQAMHPVTNDLTSWYLQDGPDNFASTYIKVVGGTTWH
                     WLGTCLRLVPNDFQLKALYGQGIDWPISYADLESFYGAAEAAIGVSGDSTSDLGAPRT
                     MPYPMPEIPQTYLDKAYVKALAGTEYDVRSTPQGRNSTDRETRPACCGNASCIPVCPV
                     QAKYDATVHLDQATAAGAVLHAKSTAVFVETGADRRVTAVRFKRWDGSEGRAIARVFV
                     LAAHAIESPRLLLNSRSEQTPGGVANSSDQVGRNLMDHPTQLSWALAKDPVYPYRGPL
                     STSGIENLRDGPFRRQRGAFRIEIGNDGWSWPTDAPISTAQDFANQGLRGKALDKAIY
                     DQAARHIRLAALVEQPPDPANRITLDAVQKDIYGVPLPRIAYRIDQYTQDGMAEARKA
                     HADIFTRLGASEIQHSYQAQGAGHIIGTLRMGSDSRTSVVDRDLRSHDHPNLFVLGSS
                     VFPTSATANPTLTIAALALRAVPTVKASLQG"
     gene            complement(2578..3309)
                     /locus_tag="AC16A0_02700"
     CDS             complement(2578..3309)
                     /locus_tag="AC16A0_02700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104270.1"
                     /translation="MDVAIITGGTRGIGGAIATALARRKVRLVLNGRSEDDEVRALLQ
                     GLGQLTDVRFHAGDASEASTSAELVDLAVSAFGRVDFAVPAAGGPNPGRATEISFDQW
                     MDAFRVHVHAVFHLFRAAHPALAVRGGAMLMIGSVAGRRGCPATVAYQTVKGALPQMA
                     RALARDHAHEGIRVNVIEPGIIRTRFHAAMTPEAERNNLDNRIPLRRFGTPEQVASAA
                     IELLTNPFITGETLAIDGGMSMRMV"
     gene            3609..4907
                     /gene="urtA"
                     /locus_tag="AC16A0_02705"
     CDS             3609..4907
                     /gene="urtA"
                     /locus_tag="AC16A0_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HIR3104271.1"
                     /translation="MSLTRRGFAKAAAGVAFVAGFGGVSGAWAQSADTIKVGVLHSLS
                     GTMAISETTLKDVMLMLIEEQNKKGGLLGKKLEAVVVDPASDWPLFAEKARELIAVNK
                     VAAVFGCWTSVSRKSVLPVFKELNSILFYPVQYEGEESERNVFYTGAAPNQQAVPAVD
                     YLMANEKVERWVLAGTDYVYPRTTNKILEAYLKAKGVAAEDIMINYTPFGHSDWQTIV
                     SDIKKFGSAGKKTAVVSTINGDANVPFYKELANQGIAAEDIPVVAFSVGEEELAGLDT
                     GPLVGHLAAWNYFQSVDTPENAEFIKTWRAFTKNDKRVTNDPMEAHVIGFNMWVKAVE
                     KAGTTDPDAVIDAIIGVSVPNLSGGFSAMMPNHHITKPVLIGEVQENGQFDIVWSTPG
                     LVVGDEWSDYLPESKDLIADWRAPMSCGNFNVATGKCGGA"
     gene            4978..>5768
                     /locus_tag="AC16A0_02710"
     CDS             4978..>5768
                     /locus_tag="AC16A0_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HIR3104272.1"
                     /translation="MRRLIATLILIVLMIAPARADLQSAVDALGSAKMDALEAVIKTL
                     AVEAGPVPATVLGALADGKLYVRKSDQKVFIVAEEAGGFALTDPVTGASAGSAAAGEV
                     SKIRINNALRRAIKAAAGGSALASPDPAKRLEAAQAVLKSRDASALPAIDAALAQETD
                     PNVKAALQLAQAATLLGSDRPEAEKIAAISTLAATGSRDVLPVLAGAAEGQGEVALAA
                     RNAISGIETSLAVWNMGQNIWFGISLGSVLLLAAVGLAITFGVMGV"
BASE COUNT         1040 a         1880 c         1822 g         1026 t
ORIGIN      
        1 cattgatggt ctttcccacc ggctcggcag cactggcagc tgttgcgaca acaagacttt
       61 cgagcgccaa aagcgcgata agtaacctca tcacctgatc tccagaattg cagccgcaag
      121 cacccaatta gacaaaggta ctttttcccc cgttggttgt ctagcctaac taatgtgcaa
      181 tttgatcatc ttcgttccgg tgcttcggcg tttctcttat ctgttccgcg gctcccccat
      241 cggaacggac cagattttta ttgctattgt agtcttttta tcctaaattg cttcgtgatg
      301 aagggaggag cgaatgggac tgggtgtttg gtgtttcgga tggcttgtca ttctgcttcg
      361 caagctgggt cttgggcctg cgttgccagc cccgcacgcg cgcgaaagac ggccgatgtc
      421 cttcgttggc gctcactgtg ttgtcctggc tggtgatctg cactacagtc gcccggaggt
      481 tgaaatcggt catgcgcctg gagaaatgcc catcatgacg tcgcgccgtg acatgatcag
      541 gggcctcctc gccggcatcc tgtcggcggc cggtgccgcg cccgtggacc atgctgcggg
      601 tgaaacgcca aaggcggcag acttcattgc cttctcggaa cggctcatgg aggtaaaggg
      661 tctcgacccc ggcatgggcg cggacatcct gggggctttc gtcgatgtcg gccagggcga
      721 ggcgctggct tcgctgatga cgcaaaccgg cccgatcgat gccggaaaca ccatggccaa
      781 tgcggtggtt gccgcctggt actcgggcct ctcgacccgc acagcaggcg agatcgtgac
      841 cgacttcaac ggcgctctca tgtgggatgc gctcagcttc accaagccct ggggatcctg
      901 cggcggcgcc acaggctact ggggcgaccc gcccagcggc tgaaccggaa cggggaccga
      961 gatggcagac gcaatcagcg ccgatgtcgt gatcatcggc tcgggcatat cgggcgcgat
     1021 gctgtcggct ggcctggcca aggccggcgt gaaggtcgcc attctcgagg cgggcgacca
     1081 ggtcgaccgg gcgacggcgg tgaacaccta ctggaatgcc gccatcaagg tccccgagtg
     1141 cgcctacccg ccgacagcgc aggccatgca cccggttacg aacgacctca cttcctggta
     1201 tctccaggat ggccccgaca atttcgccag cacctacatc aaggtggtgg gcggcaccac
     1261 ctggcactgg ctcggcacct gcctgcgcct ggttcccaat gactttcagc tcaaggcgct
     1321 ctacggccag ggcattgact ggccgatctc ctatgccgat ctcgagtcct tctatggcgc
     1381 ggcggaagcg gccatcggcg tgtcgggcga ctccaccagc gatctcggcg cgccgcgcac
     1441 catgccttat cccatgccgg agattcccca gacctatctc gacaaggcct atgtgaaggc
     1501 cctggcgggt acggaatatg atgtccgctc gacgccgcag gggcgcaatt cgaccgatcg
     1561 cgagactcgt ccggcctgct gcggcaatgc aagctgcatt ccggtctgtc cggttcaggc
     1621 gaaatacgat gccaccgtcc atcttgatca ggcgaccgcc gcgggcgccg tactgcatgc
     1681 caagtcgacc gccgtctttg tggagaccgg cgccgaccgc agggtgaccg ccgtccgctt
     1741 caagcgctgg gacggcagtg aaggccgtgc catcgccagg gttttcgtgc tggccgccca
     1801 tgccatcgag agcccgcgcc ttctgctcaa ttcgaggagc gagcagacgc ccggcggtgt
     1861 cgccaattcc tccgatcagg tcggccgcaa cctgatggat catcccaccc agctgtcctg
     1921 ggccctggcg aaggatccgg tctatcccta tcgcggcccg ctctcgacat cgggcatcga
     1981 gaacctccgc gacggcccct tccgcaggca gcgcggcgcc tttcgcatcg agatcggcaa
     2041 tgatggctgg tcctggccca ccgacgcgcc gatttcaacc gcccaggatt tcgccaacca
     2101 gggtctgcgc ggcaaggcgc tggacaaggc catctacgat caggcggcac gccatatccg
     2161 cctcgccgcg ctggtggaac agccgccgga ccccgccaat cgcatcaccc tcgatgccgt
     2221 gcagaaagac atctacggcg tgccgctgcc gcgcatcgcc tacaggatcg accagtatac
     2281 gcaggatggc atggcggaag cccgcaaggc gcacgccgac atcttcacgc gcctcggggc
     2341 cagcgagatc cagcacagct accaggcgca gggcgcgggc cacatcatcg gcacgctgcg
     2401 catgggcagc gattcccgga cctcggtggt cgacagggat ctgcgcagcc acgaccatcc
     2461 caatctcttc gtactggggt catcggtctt tccgacctcg gccaccgcca acccgaccct
     2521 gaccattgcg gccctcgccc tgcgggccgt gccgacggtg aaggccagcc tgcagggcta
     2581 gaccatccgc atcgacatgc cgccgtcgat cgccagtgtc tcgccggtga tgaagggatt
     2641 ggtcagcagc tcgatggcgg ctgatgccac ctgctcgggc gttccgaagc ggcgcagcgg
     2701 aatgcggttg tcgagattgt tgcgctcggc ctccggcgtc atggcggcat ggaagcgcgt
     2761 gcggatgatc ccgggttcga tgacattgac ccggatgcct tcatgcgcat ggtcgcgcgc
     2821 cagggcccgc gccatctgcg ggagcgcgcc cttcaccgtc tggtaggcca ccgttgcggg
     2881 acagccccgc ctgcctgcta ccgatccgat catcaacatg gcgccaccac gcacggccag
     2941 cgccggatgc gccgcgcgga agagatggaa cacggcatgc acatgaaccc ggaaggcgtc
     3001 catccactgg tcgaaggata tctcggtggc gcggcccgga ttcgggccgc ctgcggcagg
     3061 cacggcgaaa tccacgcgcc cgaaggccga aacggcgagg tcaaccagct cggcagatgt
     3121 gcttgcctcg ctggcatcgc cggcatgaaa ccggacatcg gtcagctgtc cgaggccctg
     3181 cagcagcgct cgcacctcgt catcctcact gcggccgttg agcacaagcc gcaccttgcg
     3241 ccgtgccagg gccgtggcga tggcgccccc gatgccgcgg gttccgccgg tgatgattgc
     3301 aacgtccatg gccaagggct ccttcgctta tgtaagtagg tgttgcttag tcttccgatg
     3361 cgggccttcg ccctggctgc gcccaatctt tgggcgccgc ctaatcccgc gccgccagaa
     3421 atgtgacact gcacgatcat tgtgcaagtg caaaatcgac agggctttgg aaagactctg
     3481 agatttgagc gattctccgt tcctgagcaa agcactagca gattcttttt gcgctgcagc
     3541 ggaagtggca cgtcacttgc gaagacgttc cacggttcga atgacaacaa accatggagg
     3601 gaccaggcat gtcattgaca cggagaggtt ttgcaaaggc ggcggcgggc gttgccttcg
     3661 tggcggggtt cgggggcgta agcggggcat gggcgcaaag cgccgacacg atcaaggtcg
     3721 gggtgctgca ttcgctgtcg ggcaccatgg cgatttccga gacgacgctc aaggacgtca
     3781 tgctgatgct catcgaagag cagaacaaga agggcggcct gctcggcaag aagctcgagg
     3841 ccgtggtcgt cgatccggcc tccgactggc cgcttttcgc cgaaaaggcg cgcgagctca
     3901 tcgcggtgaa caaggttgcc gccgtcttcg gctgctggac ctcggtgtcg cgcaagtcgg
     3961 tgctgccggt cttcaaggag ctcaacagca ttctcttcta tcccgtgcag tatgaaggcg
     4021 aggagagcga gcgtaacgtg ttctataccg gcgctgcacc gaaccagcag gccgttcccg
     4081 ccgtcgatta cctgatggcc aacgagaagg tcgaacgctg ggtgctggcc ggcaccgact
     4141 atgtctatcc gcgcaccacc aacaagatcc tcgaggccta tctcaaggcc aagggcgtcg
     4201 ccgccgaaga catcatgatc aactatacgc cgttcggaca ttccgactgg cagaccatcg
     4261 tctccgacat caagaagttc ggcagcgcgg gcaagaagac agccgtggtc tccaccatca
     4321 atggcgacgc caacgtgccc ttctacaagg agctggccaa ccagggcatt gccgccgagg
     4381 atattccggt cgtcgccttc tcggtgggtg aggaggaact cgccggtctc gacaccggcc
     4441 cgctcgtcgg ccacctcgcg gcctggaact acttccagtc cgtcgacacg ccggagaatg
     4501 ccgagttcat caagacctgg cgcgccttca ccaagaacga caagcgcgtc accaacgatc
     4561 cgatggaagc gcatgtcatc ggcttcaaca tgtgggtcaa ggccgtcgag aaggccggca
     4621 cgaccgatcc cgacgcggtg atcgatgcca tcatcggcgt gtcggttccc aacctctccg
     4681 gcggcttctc tgcgatgatg ccgaaccatc acatcaccaa gccggtgctg atcggcgaag
     4741 tgcaggagaa cggccagttc gacatcgtgt ggagcacgcc gggcctcgtc gtcggcgacg
     4801 aatggtcgga ctatctgccg gagtcgaaag acctgatcgc cgattggcgc gcgcccatgt
     4861 cctgcggcaa cttcaacgtc gccaccggca agtgcggcgg cgcctgatcg cctgaatacc
     4921 aaccgctccg gccgcccctg acggtggccg gagcgacgtc ctttccggag acgcgacatg
     4981 cgccgcctca tcgcgaccct cattctcatc gtgctgatga tcgctccggc ccgcgccgat
     5041 ctgcagagcg cggtggatgc gctgggctct gccaagatgg acgcgctcga ggcggtcatc
     5101 aagacgctgg cagtggaggc cggtccagtt ccggcgacgg tgctcggcgc gctcgcggac
     5161 ggaaagctct atgtccgcaa gtccgatcag aaggtcttca tcgtggccga agaggccggc
     5221 ggattcgccc tgaccgaccc ggtcacgggc gccagtgccg gaagcgctgc ggcgggagag
     5281 gtcagcaaga tccgcatcaa caacgccctg cgccgcgcca tcaaggcggc ggccggcggc
     5341 agcgcgctgg cgagtcccga tccggcgaag cgccttgagg ccgcgcaggc cgtgctcaag
     5401 tcgcgcgatg caagcgccct gccggcgatc gacgcggccc tggcgcagga aaccgatccg
     5461 aacgtgaagg ccgcgctgca attggcgcag gccgcaacgc tgctgggttc cgatcgtcca
     5521 gaggcggaga agatcgcggc gatcagcacg ctggccgcca ccggcagccg cgatgtgctt
     5581 ccggttctcg caggggcggc agaaggccag ggtgaggtgg cgctggccgc ccgtaacgcg
     5641 atctccggca tcgagaccag ccttgccgtc tggaacatgg gccagaacat ctggttcggc
     5701 atctcactgg gctcggtgct gctgctggcg gcggttggcc ttgcgatcac cttcggcgtg
     5761 atgggcgt
//
LOCUS       DBNGVR010000124         1921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4365472, whole genome shotgun sequence.
ACCESSION   DBNGVR010000124 DBNGVR010000000
VERSION     DBNGVR010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4365472"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="AC16A0_02715"
     CDS             complement(<1..114)
                     /locus_tag="AC16A0_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024796.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HIR3104273.1"
                     /translation="MKSFDLDKTDKIILTELQRDGQQAVAELAAKARLSPSA"
     gene            236..1351
                     /gene="ald"
                     /locus_tag="AC16A0_02720"
     CDS             236..1351
                     /gene="ald"
                     /locus_tag="AC16A0_02720"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR3104274.1"
                     /translation="MLIGVPKEIKNHEYRVGMTPMSVREAVRQGHKVWVQANAGAGIG
                     ASDADYTAAGAMIIPAAAEIFAKADMIVKVKEPQAVERAMLREGQILYTYLHLAPDPD
                     QTRDLVKSGAVCIAYETVTSPRGGLPLLAPMSQVAGRMSVQSGAHCLEKAQGGRGVLL
                     GGVPGVAPAKVVILGGGVVGTNAAAIALGMGADVTILEKSTDRMEELVARFGTQLKTI
                     YSTQGAVEDECATADLVIGGVLIPGAAAPKLVTRAMLKDWKPGSVLVDVAIDQGGCAE
                     TSKATTHAEPTYVVDNVIHYCVANMPGGVARTSTYALNNVTLPFALAIANKGWKKALA
                     DDAHLRNGLNVALGKVTYKAVADDLGYDYTAPDKVAA"
     gene            complement(1431..>1921)
                     /locus_tag="AC16A0_02725"
     CDS             complement(1431..>1921)
                     /locus_tag="AC16A0_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3104275.1"
                     /translation="IRGDKFVRTFDNGDRCLISEEDMEISPSKHRNQRYEPWFEERAT
                     MHRLKPGDALHMPYMIPHWVSTGATYSISMAMTWKTPEVKRLNKIRLMNGTLRRFGLP
                     QKPPGVSPALDATKVLAHDAMRAVIDPLRKSESMRRMLRGLIYGRKANYYYDTKAEKA
                     GM"
BASE COUNT          337 a          641 c          600 g          343 t
ORIGIN      
        1 ggcggacggc gaaagcctgg ccttcgcggc gagctcggcc acagcctgct gcccgtcacg
       61 ctgcagctct gtcaggatga tcttgtccgt cttgtccaga tcaaaggatt tcatgtgaag
      121 tttctagcat atcttcgaat ggaattccaa tgctgcactg ctccgggctt ctcttcgaaa
      181 attcattcga gcggttccgg ggtatgttcc gcaccatctc agggaggact gaaacatgct
      241 catcggcgta ccgaaggaaa tcaagaatca cgaataccgc gtcggcatga cgcccatgtc
      301 ggtgcgcgaa gccgtgcgcc agggccacaa ggtctgggtg caggccaatg cgggcgccgg
      361 catcggcgcc tcggacgccg actataccgc cgccggcgcc atgatcatcc cggctgccgc
      421 cgagatcttc gccaaggccg acatgatcgt gaaggtcaag gaaccgcagg cggtcgagcg
      481 cgccatgctg cgcgaaggcc agatcctcta cacctatctc catctcgcgc ccgatccgga
      541 ccagacccgc gatctggtca agtccggtgc cgtctgcatc gcctacgaga ccgtaacctc
      601 gccgcgcggc ggcctgccgc tgctggcccc catgtcgcag gttgcaggcc gcatgtcggt
      661 ccagtccggc gcccattgcc tcgagaaggc ccagggcggc cgcggcgtgc tgctcggcgg
      721 cgttccgggc gtggctcccg ccaaggtggt gatcctgggc ggcggcgtgg tcggcaccaa
      781 tgccgcggcc atcgcgctgg gcatgggtgc tgacgtgacc atcctcgaga agagcaccga
      841 ccgcatggaa gaactggtgg cgcgcttcgg cacccagctc aagaccatct attcgacgca
      901 gggcgcggtc gaagacgaat gcgccacagc cgatctcgtc atcggcggcg tgctgatccc
      961 cggcgccgcg gcgcccaagc tcgtcacccg cgccatgctg aaggactgga agcctggttc
     1021 ggttctcgtc gacgtcgcca tcgaccaggg cggctgcgcc gagacctcca aggccaccac
     1081 ccatgccgag ccgacctatg tggtcgacaa cgtcattcac tactgcgtgg ccaacatgcc
     1141 gggcggcgtc gcccgcacct cgacctatgc actgaacaac gtcaccctgc ccttcgcgct
     1201 agcgattgcc aacaagggct ggaagaaggc cctggccgac gatgcgcatc tgcgcaacgg
     1261 cctcaacgtc gccctcggca aggttaccta caaggcggtc gccgacgatc tcggctacga
     1321 ctacaccgcg cccgacaagg tcgcagcctg agacgcggtc agactccgaa tgcgaatggc
     1381 cgggaactcc cggccatttc tcgtttcaaa cgccgcttgc gcggggcagc ctacatcccg
     1441 gccttctctg ccttggtgtc atagtagtag ttggccttgc ggccatagat caggccgcgc
     1501 agcatccgcc gcatcgactc ggacttgcgc agcggatcga tgacggcgcg catcgcgtca
     1561 tgcgccaaga ccttggttgc gtccagcgcc ggagacacgc cgggcggttt ctgcggcagg
     1621 ccaaagcggc gcagcgtgcc attcatcagc cggatcttgt tgagccgctt cacttccggc
     1681 gtcttccagg tcatggccat ggaaatggaa taggtcgcgc cggtgctgac ccagtgcggg
     1741 atcatgtagg gcatgtgaag cgcgtcgccc ggcttcagcc tgtgcatggt ggcgcgctcc
     1801 tcgaaccacg gctcgtagcg ctggttgcgg tgcttggagg gggaaatctc catgtcctcc
     1861 tccgagatca ggcagcgatc gccgttgtcg aaggtgcgca cgaacttgtc gccgcggatc
     1921 t
//
LOCUS       DBNGVR010000125         1674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1419670, whole genome shotgun sequence.
ACCESSION   DBNGVR010000125 DBNGVR010000000
VERSION     DBNGVR010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1419670"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="AC16A0_02730"
     CDS             <1..695
                     /locus_tag="AC16A0_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3104276.1"
                     /translation="KLFDNDQIMNAALLSLQIAMVSATFATILGTLCGLALARFTQFR
                     GRTLFSGLVTAPLIMPEVITGISALLFFIFLAELTGWPGKRGFTTITIAHITFSMTYV
                     AVIVQSRLSSMDKSIEEAAMDLGSRPWQVLRDITLPVISPAIVSGWLLAFTISLDDVV
                     ITSFTTGPGATTLPILVWSKVKLGVTPDINALATIIIVVVGIGVAISGYLMHRADKIR
                     ERDIQMAIAANK"
     gene            692..934
                     /locus_tag="AC16A0_02735"
     CDS             692..934
                     /locus_tag="AC16A0_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104277.1"
                     /translation="MIRIETDEDARQYELLVARNTESGAALWRYGAAMYFYARGQMTA
                     DALEVYRICSPIDSEDPRQLLASRGLSQAIQRITTA"
     gene            949..1317
                     /locus_tag="AC16A0_02740"
     CDS             949..1317
                     /locus_tag="AC16A0_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3104278.1"
                     /translation="MRPVKPEDRRVVNIREGKYVPFVYEDGEALGDSILQLDDSQPLG
                     TGFHVYRMAPGMTTRPHRHNGHEQFLILEGELIENDGTVFKAGDLVWLRDGTEHCSYT
                     PNGCLLAVHIPGPEFPVDGT"
     gene            complement(1363..>1674)
                     /locus_tag="AC16A0_02745"
     CDS             complement(1363..>1674)
                     /locus_tag="AC16A0_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR3104279.1"
                     /translation="SMTAAAMGGEIEVPTIDGKKARVAIPEGTQTGKQFRLKAKGMPV
                     LRSSQFGDMYIQVAVETPVNLSKRQRELLREFDKEARNNSPESEGFFAKAKAFWDGFG
                     G"
BASE COUNT          300 a          564 c          489 g          321 t
ORIGIN      
        1 gcaagctctt cgacaatgac cagatcatga atgcggcgct gctgtcgctg cagatcgcca
       61 tggtcagcgc cactttcgcg accatcctcg gcacgctctg cggcctggcc ctggcgcggt
      121 tcacgcagtt ccgcggccgc acgctgttct ccggcctggt caccgcgccg ctcatcatgc
      181 ccgaggtgat cacaggcata tcggctttgc ttttcttcat tttcctcgcc gagctgaccg
      241 gctggccagg caagcgcggc ttcaccacca tcaccattgc ccacatcacc ttttccatga
      301 cctatgtggc cgtcatcgtg cagtcgcggc tgtcgtccat ggacaagtcc atcgaagagg
      361 ccgcaatgga cctgggctct aggccctggc aggtgctgcg cgacattacc ctgccggtca
      421 tctcgcccgc catcgtgtcg ggctggctcc tggccttcac gatctcgctc gatgacgtcg
      481 tcatcaccag cttcaccacc ggccccggtg caaccacgct cccgatcctt gtgtggagca
      541 aggtcaagct cggtgtgacg cccgacatca atgcgctggc caccatcatc atcgtggtgg
      601 ttggcatcgg tgtggcgatt tccggctatc tcatgcaccg ggccgacaag atcagggagc
      661 gcgacatcca gatggcgatc gcggccaaca aatgatccgt atcgaaaccg acgaagacgc
      721 ccgccaatac gaactgctgg tggcgcgcaa caccgagagc ggcgcagcct tgtggcgcta
      781 cggcgctgcc atgtacttct atgcgcgtgg gcagatgact gcggacgccc tcgaagtcta
      841 tcgcatctgc tcgccgatcg attccgagga tccgcgccag ctgctggcaa gccgtggcct
      901 gagccaggca atccagcgca tcacaaccgc ctgagaggaa gacttgctat gcgacccgtg
      961 aaacccgaag atcgccgcgt ggtgaacatt cgcgagggca aatatgtgcc cttcgtctat
     1021 gaggatggcg aggcccttgg cgacagcatc ctgcaactcg acgacagcca gccgctcggc
     1081 accggctttc acgtctatcg catggcaccg ggcatgacca cccggccgca tcgccacaat
     1141 ggccacgagc agttcctgat cctcgaaggc gagctcatcg agaacgacgg cacggtcttc
     1201 aaggccggtg acctggtgtg gctgcgcgac ggaacggaac attgttccta cacacccaat
     1261 ggctgcctgt tggccgtgca cattcctggg ccggagttcc cggtcgacgg cacctgatcg
     1321 gaagcgatcc tctcccatgc gcaggatggg agaggagcag actcagccgc cgaaaccgtc
     1381 ccagaaggcc ttggccttgg cgaagaagcc ctccgactcg gggctgttgt tgcgggcctc
     1441 cttgtcgaac tcgcgcagca gttcacgctg gcgcttcgac aggttgaccg gcgtttccac
     1501 cgccacctga atgtacatgt cgccgaactg cgaggaccgc aacaccggca tgcccttggc
     1561 cttgaggcgg aactgcttgc cggtctgcgt gccttccgga atggccacgc gtgccttctt
     1621 gccgtcgatg gtgggcacct cgatctcgcc gcccatggcg gccgccgtca tcga
//
LOCUS       DBNGVR010000126         1537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1027351, whole genome shotgun sequence.
ACCESSION   DBNGVR010000126 DBNGVR010000000
VERSION     DBNGVR010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1027351"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC16A0_02750"
     CDS             complement(<1..471)
                     /locus_tag="AC16A0_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3104280.1"
                     /translation="MSRKFGIVGTGAWGTALAHVARSAGHAVGLWSRGTPDPNALARA
                     DALILAVPAQALRATLTAIAPAVAARQPLIVSAKGIEQGTGLFMNDVVQQVLPHAHPL
                     VLSGPSFAADVLKGLPTAVVMAAPTLAEAGEWAGALSIPHFRIYASDDVKGVEIG"
     gene            complement(468..>1537)
                     /gene="tsaD"
                     /locus_tag="AC16A0_02755"
     CDS             complement(468..>1537)
                     /gene="tsaD"
                     /locus_tag="AC16A0_02755"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3104281.1"
                     /translation="GIETSCDETAAAVVRRESDGRGHILSNLVLSRTQDHAPYGGVVP
                     EIAARAHVSHLDGLIARAMSEAGLRFADLDGVAATAGPGLLGGVIVGLTTAKAIALAH
                     DKPLLAVNHLEGHALTARLLQPCAFPYLLLLISGGHTQFQIVAGVGRYHRLGTTIDDA
                     LGEAFDKVAKLLGLGYPGGPQVERQARAGDPRRFDFPRPLKGRKDCNFSFSGLKTAVR
                     EAVHGLGSLSPQDVADVCASFEAAVAETMADRLRMAVTLFRAAHPEVARPALIVAGGV
                     AANLKLRSVFEEAAAAHGFDLIVPPAALCTDNAAMIAWAGAERLALGLTDDLAVAARA
                     RWPLDQNQSPAYGAGRLGAKA"
BASE COUNT          250 a          518 c          560 g          209 t
ORIGIN      
        1 gccgatctcg acccccttta cgtcgtccga ggcatagatg cgaaaatgcg gaatggagag
       61 tgcgccggcc cattcccccg cttccgccag ggtcggggcg gccatgacca ccgctgtcgg
      121 caggcccttc aacacgtccg cggcgaagct tgggcccgac aggaccagcg gatgggcgtg
      181 cggcaggacc tgctgcacga cgtcgttcat gaagaggccg gtgccctgct cgatgccctt
      241 ggcgctgacg atgagcggct gacgcgccgc gacggccggc gcgatggcgg tcagcgtcgc
      301 gcgcagcgcc tgggcgggaa ccgccagaat gagcgcgtca gcgcgggcaa gggcgttggg
      361 atcgggtgtg ccgcgcgacc acagtcccac cgcatggccg gcgctgcgcg ccacatgcgc
      421 cagggccgta ccccaggcgc ccgtgccgac gatgccgaac ttgcggctca tgccttggcc
      481 ccgagcctgc ccgcgccata ggcgggcgac tgattctggt ccagcggcca gcgggcccgc
      541 gcggcaacgg cgagatcgtc ggtcagaccc aaggccaggc gttcggcccc ggcccaggcg
      601 atcatggctg cattgtcggt gcagagcgcg gccggcggca cgatgagatc gaagccgtgg
      661 gcggccgctg cctcctcgaa gaccgatctg agtttcagat tggcggcaac gccgcccgcc
      721 acgatcagcg caggccgcgc gacttccggg tgcgccgcgc ggaacagcgt gaccgccatg
      781 cgcagacggt cggccatggt ctcggccacg gccgcctcga agctggcgca gacatcggcg
      841 acgtcctgcg gcgagaggct gcccagcccg tgcacggcct cgcgcactgc ggtcttcagt
      901 ccggagaagg agaagttgca gtccttgcgg cccttcagcg gacgcgggaa atcgaaacgc
      961 cgcggatcgc cggcgcgcgc ctgccgttcc acctgcgggc cgccgggata gccgaggccg
     1021 aggagtttcg ccaccttgtc gaaggcctcg cccagggcat cgtcgatggt ggtgccaagg
     1081 cgatggtagc ggccgacgcc cgcaacgatc tggaactggg tgtggccgcc ggagatcagc
     1141 agcaaaagat aggggaaggc acagggctgg agcagccttg ccgtcagcgc gtgaccttca
     1201 agatgattga cggccagcag gggcttgtca tgggccagcg caatggcctt ggcggtggtg
     1261 aggccaacga tgacaccgcc caagagcccc gggccggcgg tggccgccac gccatcgagg
     1321 tccgcgaagc gaaggcccgc ttcggacatg gcgcgggcga tcagtccatc aaggtggctg
     1381 acatgggcgc gggcggcgat ttccggcacc acgccgccat agggggcgtg gtcctgcgtc
     1441 cgggacagca cgaggttcga caggatatgc ccccggccat cggactcgcg ccgcaccacg
     1501 gcggcggcgg tctcgtcgca agaggtttcg atgccga
//
LOCUS       DBNGVR010000127         3502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1420494, whole genome shotgun sequence.
ACCESSION   DBNGVR010000127 DBNGVR010000000
VERSION     DBNGVR010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1420494"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC16A0_02760"
     CDS             <1..525
                     /locus_tag="AC16A0_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3104282.1"
                     /translation="ERLRGLRGARGWPLDELARRSGVSRATLSRLENAEVSATATVLG
                     KLCAAYGLTVSRLMLMAEDAFAPLVRRGGQPLWQDPETGFARRSVSPPAGGLAGEVIE
                     GRLPPGVSIAYERPPRPGLEHHLVMMEGALDLTIEGRRHDLEAGDCLRYQLFGASRFE
                     TRAGTGARYLLFIL"
     gene            542..1009
                     /locus_tag="AC16A0_02765"
     CDS             542..1009
                     /locus_tag="AC16A0_02765"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3104283.1"
                     /translation="MIRIIPADLSDPRVIGLLQHHFTTCHAVTPKGSAHAVDLSGLQL
                     PEVSFWAAWDGDALLGIGALKRIAPGHGEVKSMHTAEVARRRGVAGAMLRHIIADARS
                     QGLERLSLETGSFDYFRPAVALYRAHGFVDCAPFGSYRTDPNSVFLTLDLSRA"
     gene            complement(996..1307)
                     /locus_tag="AC16A0_02770"
     CDS             complement(996..1307)
                     /locus_tag="AC16A0_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3104284.1"
                     /translation="MSIRHIDAREAKRLVDGGAILIDVREPGEHAAENIPGARNHPLS
                     AIAQSPPDPALQTVIYHCKSGGRTRMASSALSRCSAADSYIMDGGIEAWKAAGYPVKR
                     G"
     gene            complement(1324..1638)
                     /locus_tag="AC16A0_02775"
     CDS             complement(1324..1638)
                     /locus_tag="AC16A0_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3104285.1"
                     /translation="MTALLIDKSISELEVSATEAASVLKSLSNEKRLMILCKLLEEGE
                     MSVLPIAEAVGLGQSALSQHLARMRAEKIVDFRRDGQTLYYRIADPKVAQLLKSLKSI
                     FC"
     gene            1716..2588
                     /locus_tag="AC16A0_02780"
     CDS             1716..2588
                     /locus_tag="AC16A0_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3104286.1"
                     /translation="MVAAALVEAFFDEPTNTVSYLVVDPQTHRAAVVDPVLDYDQRTG
                     KASAKSADRIIDRATVLGAAIDWVLETHVHADHLSGAPYIKVKTGAKVVIGERIREVQ
                     KIFRPVFNALDVSGEGSEFDKLVKDGDRLPLGDLTIEVIATPGHTPACVSYRIGNTVF
                     VGDTLFMPDYGTARADFPGGDARALYRSIRRLLALPKETRLFMCHDYLPKGGRDHYAW
                     GTTVGDELAHNIHVHEGVSEDQFVAMRQARDATLSAPVLLMPSIQVNMRAGKLPPADA
                     NGVHYIKIPVSLAS"
     gene            2658..>3502
                     /locus_tag="AC16A0_02785"
     CDS             2658..>3502
                     /locus_tag="AC16A0_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104287.1"
                     /translation="MAEQSNLRGIGAMLLATGAFVANDSCMKLALSDAPPLQVLIMRG
                     IAACLWCLPILLILGHGRDLPKVFNRWVALRSLCEVFAILSFILALNEMPIADITAIA
                     QIAPLLVLLGIWLFFGDRIGMLRLALIGVGITGALLVAQPGSEAASPMAILGFFTALG
                     AAGRDIVTRKVPPGTPALIVTFSTLLIVMLCATVGSFAFETQVAPTFRHAWLMAIAGF
                     FLMCGHLFVFLAFRIGEARVVAPFNYSFMIWAGLSGLLIFNEVPNALAIAGMALIMLA
                     GLAVIA"
BASE COUNT          544 a         1163 c         1117 g          678 t
ORIGIN      
        1 gagcgtctgc gcgggctgcg cggcgcgcgc ggctggccgc tggacgaact ggcgcggcgc
       61 agcggtgtca gccgggccac gttgtcccgg ctcgaaaatg ccgaggtcag cgcaacggca
      121 acggtcctgg gcaagctctg cgcggcctac ggcttgacgg tctcgcgcct catgctgatg
      181 gccgaagacg ccttcgcacc tctcgtccgg cgcggcggcc agccgctctg gcaggatccc
      241 gaaaccggct tcgcgcgccg gtccgtctcg ccgcccgccg gcggcctcgc cggcgaagtg
      301 atcgaaggcc ggctgccgcc cggtgtcagc atcgcctatg agcggccgcc gcggcccggc
      361 ctcgagcatc acctggtgat gatggagggc gcgctggacc tgacaatcga gggacgccgt
      421 catgatctgg aggcgggcga ctgcctgcgc taccagctct tcggtgccag ccgcttcgaa
      481 acccgcgccg ggaccggcgc gcgctatctg cttttcattc tctgatgggc gagactttgc
      541 catgatccgc atcatccctg ccgaccttag cgacccgcgc gtcatcggcc ttctgcagca
      601 tcatttcacc acctgccacg cggtcacgcc caagggcagc gcccatgccg tggatctctc
      661 gggcctgcag cttccggaag tgagtttctg ggcggcctgg gacggggatg ctctgctggg
      721 catcggcgcg ctgaagcgca tcgcgccggg ccatggcgag gtgaagtcga tgcatactgc
      781 ggaggtcgcg cgtcgccgcg gcgtcgccgg ggcgatgctg cgccacatca ttgccgacgc
      841 ccgaagccag ggtctggaac ggctcagcct cgagaccggt tccttcgatt acttccgccc
      901 cgcggtggcg ctctaccggg cgcatggctt cgtcgactgc gcgcccttcg gatcctaccg
      961 taccgatccc aacagcgtgt tcctgacgct ggatctcagc cgcgcttgac cgggtagccg
     1021 gccgccttcc aggcttcgat gccgccatcc atgatgtagg agtcggcggc gctgcagcgg
     1081 ctcagcgccg atgaggccat gcgcgtgcgt ccgccggact tgcaatgata gatcacggtc
     1141 tgcagcgccg ggtccggcgg tgactgggcg atggcggaaa ggggatggtt ccgcgcgccg
     1201 ggaatattct ccgcggcatg ctcgccgggc tcccggacgt cgatcaggat cgcgccgcca
     1261 tcgacaaggc gcttggcttc gcgggcatcg atatgacgaa tggacatgtg gtggttccgg
     1321 ccatcagcag aaaatggatt tgagcgactt caaaagctga gccaccttgg gatcggcgat
     1381 gcggtagtaa agcgtctgcc cgtcgcggcg gaaatcgacg atcttctcgg cgcgcatgcg
     1441 ggcgagatgc tgcgacagcg ccgattggcc gaggccaacc gcttcggcga tgggcagcac
     1501 gctcatttcg ccttcctcga gcagcttgca aaggatcatc agccgcttct cgttggacag
     1561 cgacttcagg acgcttgccg cttcagtggc tgagacttcg agttcggaaa tggacttgtc
     1621 gatcaggagg gcggtcatgc ggggtccaag ataaaacttg ttaaattagg ccttactaat
     1681 atatagtcgc gaagtcaaca gtctttgagg tcgccatggt cgctgcagcc cttgtcgagg
     1741 cattcttcga tgaaccgacg aacacggtca gttatctggt cgtcgatccg cagacgcatc
     1801 gcgcagccgt ggtcgacccg gtcctcgact acgaccagcg caccggcaag gccagcgcga
     1861 aatccgccga ccggatcatt gaccgggcca cggtgctggg cgcggccatc gactgggttc
     1921 tcgaaaccca tgttcatgcg gatcatcttt ccggcgcgcc atatataaag gtcaagaccg
     1981 gcgccaaggt cgtcattggc gagcgcatcc gtgaggtcca gaaaatcttc cggcctgtct
     2041 tcaatgccct cgacgtctcc ggagaaggct cggaattcga caagctggtg aaggatggcg
     2101 accggcttcc gttgggcgat ctcactattg aagtcatcgc cacgccgggc cacacgccgg
     2161 cctgcgtgtc ctacaggatc ggcaacacgg tcttcgtcgg cgatacgctg ttcatgccgg
     2221 attacggcac ggcgcgcgcc gatttccccg gcggcgatgc ccgcgccctc tatcgttcga
     2281 ttcgccgcct gctggcgctg ccgaaggaga cgcgcctctt catgtgccat gactacctgc
     2341 ccaagggcgg ccgcgaccac tatgcctggg gaaccacggt gggcgacgag ctggcgcaca
     2401 acatccatgt ccacgagggc gtgagcgagg accagttcgt ggccatgcgc caggctcgcg
     2461 atgccacgct ctcggcgccg gtgctgctga tgccgtcgat tcaggtgaac atgcgggccg
     2521 gcaagctgcc gccggcggat gccaatggcg tccactacat caagataccg gtcagccttg
     2581 cgtcctgaac agggctcgtt tgccaagttg acgcgcggcc tgtagggagg tccggcaacc
     2641 tcatctccgg attcctgatg gctgaacagt ccaatctccg cggtatcggc gcgatgctgc
     2701 tggcgaccgg ggccttcgtt gccaatgaca gctgcatgaa actggcgctc tccgacgcac
     2761 cgccgctgca ggttctgatc atgcgcggca tcgcggcctg tctctggtgc ctgcccatcc
     2821 tgctgatcct cgggcatggc cgggacctgc ccaaggtctt caatcgctgg gtggcgctgc
     2881 gctcgctgtg cgaggtcttc gccatcctga gcttcatcct ggccctgaac gaaatgccga
     2941 ttgccgacat cacggccatt gcgcagatcg cgccgctcct ggtgctgctc ggcatctggc
     3001 tgttcttcgg cgaccgcatc ggcatgttgc gcctggcgct gatcggcgtg gggatcaccg
     3061 gcgccctgct ggtggcgcag cccggcagcg aagccgcctc gcccatggcc atcctcggct
     3121 tcttcaccgc ccttggcgcg gccgggcgcg acatcgtcac gcgcaaggtg cctcccggca
     3181 cgccggcgct gatcgtgacc ttctccacgc ttctcatcgt gatgctctgc gccacggtcg
     3241 gttcattcgc cttcgaaacg caggtcgcgc cgaccttccg ccatgcctgg ctcatggcca
     3301 ttgccggctt cttcctgatg tgcgggcacc tcttcgtctt cctcgccttc cgcatcggcg
     3361 aggcgcgggt tgtggcaccc ttcaactatt ccttcatgat ctgggccggt ctctccggcc
     3421 tgctcatctt caacgaggtg ccgaatgcgc tggccattgc gggcatggca ctgatcatgc
     3481 ttgcgggtct cgccgtcatc gc
//
LOCUS       DBNGVR010000128         2159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_438476, whole genome shotgun sequence.
ACCESSION   DBNGVR010000128 DBNGVR010000000
VERSION     DBNGVR010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_438476"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="AC16A0_02790"
     CDS             complement(<1..1820)
                     /locus_tag="AC16A0_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR3104288.1"
                     /translation="MKQTAQDSTFSRTKLKLAFATIGVVFGDIGTSPLYAMREALHPV
                     VAAGGDLRAAVLGVASLLIWALILIVTLKYVFFLMRADNRGEGGILSLVVLVETLLKT
                     KSGFVLTLGMIGAAFFFGDAMITPAMSVLSAVEGLKVINTGLSPFVVPITLVILISLF
                     LFQYKGTAGVASLFAPITAIWFLTIGVMGFYHIFDDLQIFLAFNPTYGASMLIERRFL
                     ALLVFGGVFLAVTGGEELYADMGHFGSKPIRMAWLAVVFPGLILNYLGQGAFIISHPE
                     AVENPFYLMAPGWGLIPLVILATAVTVIASQAVITGAFSIAQQAMSLGLFPRMSITHT
                     SETEQGQIYVAQINWMLLAGVVILVLVFKSSTNLASAYGIAVNTSMIVDSILALVFFW
                     KSRNLPRWFVIPAMTVILFVELTFMAANGLKLFTGGYMPALLGATIILLMITWMKGRK
                     ALAEKLRRESIELVPLLESLEKRPPTRVAGAAIFLQTDPIYAPSALMHTLKHNRVLHD
                     RLVFITAETTTEPRIDSEEERVIVKQLPLGAWLVEARFGYMEQPDVPKALRACAPFGL
                     EIDPRQASYFLGRRVIRMSARSSLPFWQQRLFIMMANQSAR"
     gene            complement(1867..>2159)
                     /locus_tag="AC16A0_02795"
     CDS             complement(1867..>2159)
                     /locus_tag="AC16A0_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HIR3104289.1"
                     /translation="DTAWLAKVRPYFGHNYHFHIRINCPKGSTTCKNQPTPHPKDGTG
                     CGEELAYWMGDVPWVPKKKPDPNAKPAKPVKPPPPMTVSALPAECRTVVTAE"
BASE COUNT          415 a          628 c          749 g          367 t
ORIGIN      
        1 cgcgcggact ggttcgccat catgatgaac agccgctgct gccagaacgg cagcgacgag
       61 cgcgccgaca ttcttatgac gcgacggcca aggaaatatg acgcctgccg cggatcgatt
      121 tcgaggccga agggcgcgca ggccctgagc gccttgggca cgtccggctg ctccatgtag
      181 ccgaagcgcg cctcgaccag ccaggcgccc agcggcaact gcttgacgat gacgcgttcc
      241 tcttcgctgt cgatgcgcgg ctcggtggtg gtttccgcgg tgatgaagac cagccggtca
      301 tgcagcacgc gattgtgctt gagagtgtgc atgagcgcgc tcggcgcata gatcgggtcg
      361 gtctgcagga agatcgcggc gccggccacg cgggtcggcg gccgcttctc gaggctttcg
      421 agcagtggca cgagttcgat cgactcccgc cgcagcttct cggccagggc cttgcgtccc
      481 ttcatccagg tgatcatcag caggatgatg gtggcaccta gcagcgccgg catgtagccg
      541 ccggtgaaga gtttcagccc attggcggcc atgaaggtga gttcgacgaa gaggatgacg
      601 gtcatggcgg ggatcacgaa ccagcgcggc aggttccggg acttccagaa gaagaccagc
      661 gcgaggatcg aatcgacgat catagaggtg tttacggcga tgccataggc cgaggcgagg
      721 ttggtggacg acttgaagac cagcaccagg atgaccacgc ccgccaagag catccagttg
      781 atctgggcaa cgtagatctg tccctgttcg gtctcgctgg tatgggtgat gctcatgcgc
      841 gggaacagcc ccagcgacat ggcctgctgg gcgatggaga aggcgccggt gatcacggcc
      901 tgcgaggcga tcaccgtgac cgccgttgcc aggatgacga gcggaatgag tccccagccc
      961 ggcgccatca ggtagaaggg attctccacg gcttccggat gcgagatgat gaaggcgcct
     1021 tgcccaaggt agttcaggat caggccgggg aagacgacgg caagccaggc catgcggatc
     1081 ggcttgctgc cgaagtggcc catgtcggcg tagagctcct cgccgccggt caccgccagg
     1141 aagacgccgc cgaagacgag cagcgccagg aagcgccttt cgatcagcat gctggcgcca
     1201 taggtcggat tgaaggcaag gaagatctgc aggtcgtcga agatgtgata gaagcccatc
     1261 acgccgatgg tgagaaacca gatggcggtg atcggcgcga acagcgaggc cacgcccgcc
     1321 gtgcccttgt actggaacag gaagaggctg atcaggatca ccagcgtgat cggcacgacg
     1381 aagggcgata acccggtgtt gatgaccttg aggccttcca cggccgagag caccgacatg
     1441 gctggcgtga tcatggcatc gccgaagaag aaggccgcgc cgatcatgcc gagcgtcagg
     1501 acgaatccgc tcttggtctt gagcagcgtt tcgaccagca cgacgagcga gaggattccg
     1561 ccttcgccgc gattgtcggc ccgcatgagg aagaagacgt acttcagcgt gacgatcagg
     1621 atcagcgccc agatcagcag cgaggcaacg cccaggacgg cagcgcgcag gtcaccgccg
     1681 gcggccacca cgggatgcag ggcttcgcgc atggcataga gcgggctggt tccgatgtcg
     1741 ccgaagacca ctccgatcgt ggcgaaggcg agcttcagct tggtgcggct gaaggtggag
     1801 tcctgggcgg tctgtttcat tggcgcgccg ccttggacca tctccccggg caggtcaagg
     1861 gcttgactat tcggcagtga cgacggtgcg gcattcggcc ggcagggccg aaaccgtcat
     1921 gggcggcggc ggcttcacgg gcttcgccgg cttggcgttg ggatcgggct tcttcttcgg
     1981 gacccagggc acatcgccca tccagtaggc aagttcctcg ccgcagccgg tgccgtcctt
     2041 cggatgcggc gtgggctggt tcttgcaggt ggtcgaaccc ttcgggcaat tgatgcggat
     2101 gtggaagtgg tagttgtgcc cgaaataggg tcggaccttg gccagccacg ccgtgtcgc
//
LOCUS       DBNGVR010000129         2781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4170026, whole genome shotgun sequence.
ACCESSION   DBNGVR010000129 DBNGVR010000000
VERSION     DBNGVR010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4170026"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC16A0_02800"
     CDS             <1..519
                     /locus_tag="AC16A0_02800"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR3104290.1"
                     /translation="TAPTNGAAGVMPATIRYYLDHCIGADPAGIRTFLLAASAVGGII
                     KHNASISGAEVGCQGEVGSASAMAAAGLCAALGGSNEQVENAAEIALEHHLGMTCDPV
                     GGLVQVPCIERNAFGAVKAVTAASLALRVDGTHFDPLDSCVETMRQTGIDMIERYKET
                     STGGLAVNVVEC"
     gene            524..2275
                     /locus_tag="AC16A0_02805"
     CDS             524..2275
                     /locus_tag="AC16A0_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3104291.1"
                     /translation="MPVYAAPLREHLFCLYDLLGFDAHCALPGFGDISRDVVEAVLEQ
                     SSRFATQTLLPLNRPADEQGCRLAEGVVTTPEGFKSAYRKLVEGGWPALTCAAAHGGQ
                     GFPHMINVLFEEMMNAACLSFGLFPGLGRGAYVAIARHGSPEQAALYAPRLASGVWAG
                     SMCLTEAQAGTDLGLLRTQAVPQRDGSYAITGTKTFISCGEHDLTSNIVYLVLAKLPG
                     APAGTRGISLFLVPKFIPDSLGNPGPRNGVSCGAIERKMGLHGSPTCVMNFDGATGWL
                     IGEPNKGLAAMFSMMNAERIAVGIQGLAIAEASYQNALAYAHVRHQGRAPGSEAVADP
                     IIRHPDVRRMLMTQRVLVEGGRALALWTAQALDVSERHADPQVRQQADEFNALMTPVV
                     KAFLTDIGSECASLGVQVWGGHGYMRDNGMEQFVRDVRVTQIYEGTNGVQALDLLGRK
                     VLQLGLLDRFTVPAAAELAEASTDDRLRHLAGPALSAIGLLKESTSLLHLRASDSAEV
                     GSAATEYLRLFALTALGYLWTRMARLAVAAGDDGFTSAKLAGAEFYITRILPQTAALH
                     AAVAAGAVPVMNPAALH"
     gene            complement(2337..2585)
                     /locus_tag="AC16A0_02810"
     CDS             complement(2337..2585)
                     /locus_tag="AC16A0_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104292.1"
                     /translation="MKTIVLSLAVLTTLSSASYASWRDRTPNESRFGFANEQASETSG
                     TMDLQTEGFMVEGAASTPAMPGAGKNVDPYRFNWGGRS"
BASE COUNT          461 a          912 c          882 g          526 t
ORIGIN      
        1 accgcgccca ccaatggggc tgcaggtgtc atgcccgcca ccatccgcta ctatctcgat
       61 cattgcatcg gcgccgatcc tgccgggatc cggaccttcc tgctggcggc ttcggccgtc
      121 ggcggcatca tcaagcacaa tgcctcgatc tcgggcgccg aggtcggctg ccagggcgaa
      181 gtgggttcgg cctcggccat ggcggcggcg ggcctctgcg ccgcgctcgg cggtagcaac
      241 gagcaggtgg agaacgcggc cgagattgcg ctcgaacacc acctcggcat gacctgcgat
      301 ccggtgggcg ggctggtgca ggtgccctgc atcgagcgca atgccttcgg tgcggtgaag
      361 gccgtcaccg ccgcgtcgct ggcgctgcgc gtcgacggca cgcatttcga tccgcttgac
      421 agctgcgtcg agaccatgcg tcagaccggc atcgacatga tcgagcgcta caaggaaacc
      481 tccaccggcg gcctcgccgt caacgtggtc gagtgctagg tccatgcccg tctatgccgc
      541 gccgctgcgc gaacatctct tctgcctcta cgaccttctc ggattcgacg cgcattgcgc
      601 tctgcccggc ttcggcgaca tctcgcgcga tgtggtcgaa gccgtgctgg agcaatcatc
      661 gcgcttcgcc acgcaaacgc ttctcccgct gaatcgaccg gccgacgaac agggctgccg
      721 tctggcggag ggcgtcgtga ccacgcccga gggattcaag tctgcatatc ggaaacttgt
      781 ggagggcggc tggccggcgc tcacctgcgc ggctgcgcac ggcggccagg gctttcccca
      841 catgatcaac gtcctcttcg aggaaatgat gaatgcggcc tgcctgtcct tcgggctctt
      901 tcccggcctt ggccgtggcg cctatgtcgc catcgcgcgc cacggcagtc ccgaacaggc
      961 ggcgctctat gcgccaaggc tcgcgagcgg cgtctgggcc ggctccatgt gcctgaccga
     1021 ggcacaggcc ggcaccgatc ttggcctgct gcggacgcag gcggttccgc agcgcgacgg
     1081 cagctatgcc ataaccggaa ccaagacctt catctcctgc ggcgagcacg acctcacgtc
     1141 aaacatcgtg tatctcgtgc tggcaaaact tcccggcgcg cccgcgggca ccaggggcat
     1201 cagcctgttc ctcgttccca agttcattcc ggattcgctc ggcaaccccg gccctcgcaa
     1261 cggcgtgtcc tgcggcgcca tcgagcgcaa gatggggctc cacggatcgc ccacctgcgt
     1321 gatgaacttc gatggcgcta ccgggtggct cattggcgag ccgaacaagg gcctggccgc
     1381 aatgttctcc atgatgaacg ccgagcgcat cgcggtaggc atacagggac tggcgattgc
     1441 cgaggcctcc taccagaatg cgctggccta tgcccatgtg cgccaccagg gccgtgcccc
     1501 gggttcggag gcggtcgctg atcccatcat ccgccatccc gacgtgcgcc gcatgctgat
     1561 gacccagcgc gtgctcgtcg aaggcggccg cgccttggca ctttggacgg cccaggcgct
     1621 ggacgtgtcg gagcgccatg ccgatccgca agtccgtcag caggctgatg aattcaatgc
     1681 gctcatgacc ccggtggtca aggcattcct cacggatatc ggcagcgaat gcgcaagtct
     1741 tggcgtgcag gtctggggcg gtcacggcta tatgcgcgac aacgggatgg aacaattcgt
     1801 ccgtgatgtc cgggtgacgc agatctacga gggtaccaat ggcgttcagg cgctggacct
     1861 tctcggccga aaggtgctgc aactgggatt gctcgaccgc ttcacggtac cggctgccgc
     1921 cgaacttgcc gaggcttcga cggacgacag gctgcggcac ctcgccggcc cggcgctctc
     1981 cgcgatcggc ctgttgaagg agtcaaccag cctgctgcac cttcgcgcgt ccgattcggc
     2041 cgaagtgggg tccgcggcca ccgaatactt gcggcttttc gcgttgactg cgctcggcta
     2101 tctctggacc cgcatggctc gccttgccgt ggccgctggc gatgatggct tcacgtctgc
     2161 caagctggct ggtgccgagt tctacatcac ccgcatcctg ccgcagaccg cggcgcttca
     2221 cgcggccgtt gcagcgggtg ccgttccggt catgaatcct gctgccctgc attgagcctg
     2281 aacgggcaag agccggctgc gctggcagcc ggctctctgg tgcttctggt ttgtgcttac
     2341 gaacggccgc cccagttgaa gcgataggga tcgacgttct tgccggcgcc aggcatggcc
     2401 ggggtgctgg cagcaccttc gaccatgaag ccctcggttt gcagatccat ggtgcccgag
     2461 gtttcggaag cctgctcatt ggcgaatccg aagcggctct cgttcggcgt gcggtcgcgc
     2521 cacgaggcat aggaggcaga cgacagggtg gtcaggacgg cgaggctcag gacgatggtc
     2581 ttcatggcga actccttcgg ggtttgaggt ttcgggtatc tctttcggtc agcgaatgct
     2641 gcgtccgatg tcatcaagat agccctccac ccgccagaat cgaatcaacc ccgttcacgc
     2701 tccgatcaga agaaaacata tataaaacaa tatattacag ttggatttgt ggtgcggatt
     2761 caaacatcgt tcaagccgcc a
//
LOCUS       DBNGVR010000130         4575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1814578, whole genome shotgun sequence.
ACCESSION   DBNGVR010000130 DBNGVR010000000
VERSION     DBNGVR010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1814578"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="AC16A0_02815"
     CDS             <1..997
                     /locus_tag="AC16A0_02815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3104293.1"
                     /translation="ELIKPNKLEVASGDDPKRVATVVAEPLERGFGTTLGNALRRILL
                     SSLQGAAVTSVLIDGVLHEFSSIAGVREDVTDIILNIKEIAIGMQADAPKRLVLRKEG
                     PGVARAGDIQQTGDIQILNPEQVICTLDEGAEVRMEFTVATGKGYVPSERNRPEDAPI
                     GLIPVDSIYSPVKRVSYKIENTREGQILDYDKLIMTVETDGSMTPEDAVAYAARILQD
                     QLSVFINFSEPSKAVIEESRPELEFNAALLKKVDELELSVRSANCLKNDNIVYIGDLI
                     QKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVPNWPPENIEELAKKYETHY"
     gene            1043..1468
                     /gene="rplQ"
                     /locus_tag="AC16A0_02820"
     CDS             1043..1468
                     /gene="rplQ"
                     /locus_tag="AC16A0_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR3104294.1"
                     /translation="MRHGFRGRRFNRTAEHRTAMFANMSAALIKHEQIVTTLPKAKDL
                     RPVVEKLVTLAKKGSLHARRQAMAEVRDETQVKKLFEVLAARYKDRHGGYLRIMKAGF
                     RYGDSAPMAVIEFVDRDVNARGQDSGPSNKPEEAAEAAA"
     gene            1631..2353
                     /locus_tag="AC16A0_02825"
     CDS             1631..2353
                     /locus_tag="AC16A0_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3104295.1"
                     /translation="MIPRFDLFNEARQPFASARESSEYLKRARDRFSVQNLSYWFLGS
                     SPQMPDRLTWLSTYDQEYMAVYMREYSPLNDPVFDVCFRRLLPLDWHEMRDADQQAQM
                     IHQVAEQYGIGRHGISFPIRDPGFGDAMFSVNFECDDRQWDSLRHDLVNGFHLFAHFF
                     HLRMKDVISRRPVTSEFDLSPREREVLAWAADGKTAWETAQLLGVSERAIRLYTENAM
                     NKLRAKTKTQAVAIAVKNDIIH"
     gene            complement(2354..3538)
                     /locus_tag="AC16A0_02830"
     CDS             complement(2354..3538)
                     /locus_tag="AC16A0_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme domain-containing
                     protein"
                     /protein_id="HIR3104296.1"
                     /translation="MTATVRKLEKAGRFADARAVARILQPEEPVFLFSAPQLLARLKT
                     FQKGFPGTTSFAVKSNPSPDVIACLAKAGLKHWDVASVHEMEAVRAQAPKPVFHYHNP
                     VKSRREIADAYGRYGCRRFAVDCREELAKIADIAGSDASLEIAVRFVLPRDRGSSAHD
                     FSTKFGAPEHVAVALLEEAARRGYRPLLTFHPGSQTRDPQTYVRHIEAAARIAAAAGI
                     TLSKLNVGGGFPADYPNSKGGPLEIFFHTIGQAVTRCFGAGREPQLECEPGRGLVANA
                     MSLLTRIKLVCSDGDDIFINDGVYGGLMEFMQVPELQPPHRVIRDGEVLAGPAKPWKV
                     FGPTCDPLDVLPHRLDLPAHIMDEDFIEFGTLGAYGIATSTMFNGYGGHRVIPVETAL
                     AT"
     gene            3662..4099
                     /locus_tag="AC16A0_02835"
     CDS             3662..4099
                     /locus_tag="AC16A0_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR3104297.1"
                     /translation="MVQITPKDEQLIALLRVDAREPVASLARKLGLSRTTVQDRLRRL
                     EGSGIIAGYSLKLAREIDAGGMRAFVTLSIEPRRQVEVARTLAKFHQIETLHTVSGKY
                     DLVAQVKTGTSEDMDRLIDEIGLIPGITRIETAVILSTKLDRR"
     gene            4127..>4575
                     /locus_tag="AC16A0_02840"
     CDS             4127..>4575
                     /locus_tag="AC16A0_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3104298.1"
                     /translation="MRLIHLALAAVIALAVPPAVRAETQVPRSQGEMLLSFAPVVKRT
                     APAVVNVYAQRIVASRQEGIGADPFFRRFFGENGGFGRSRERVQNSLGSGVIVDPSGI
                     IVTNNHVIRGGSEIKVVLADRREFEASVVLTDERTDLAVLRIDVSDE"
BASE COUNT          873 a         1412 c         1480 g          810 t
ORIGIN      
        1 agagctgatc aagccgaaca agctcgaagt cgccagcggc gacgatccca agcgtgtcgc
       61 caccgtcgtg gcggagccgc tggagcgcgg cttcggcacg acgctcggca atgcgctgcg
      121 ccgcatcctc ttgtcgtcgc tgcagggcgc ggccgtcacc tcggttctca tcgacggcgt
      181 gctgcacgag ttctcctcga tcgccggcgt gcgcgaggac gtgaccgaca tcatcctcaa
      241 catcaaggaa atcgccatcg gcatgcaggc cgatgcgccg aagcggctgg tgctgcgcaa
      301 ggaaggcccg ggtgtcgccc gcgccggcga tatccagcag accggcgata tccagatcct
      361 caatccggag caggtcatct gcacgctgga cgagggtgcg gaagtccgca tggagttcac
      421 cgttgccacc ggcaagggct atgtcccctc cgagcgcaac cgtccggaag atgcgccgat
      481 cgggctgatc ccggtcgact cgatctacag cccggtcaag cgcgtgtcct acaagatcga
      541 gaacacccgc gaaggtcaga tcctcgatta cgacaagctg atcatgaccg tcgagaccga
      601 cggctcgatg accccggaag atgctgtggc ctatgctgca cgtatcctgc aggaccagct
      661 ctcggtcttc atcaatttct cggaaccttc aaaggccgtg atcgaggaga gccgtccgga
      721 gctcgagttc aacgccgccc ttctcaagaa ggtcgacgag ttggagcttt cggtgcgttc
      781 ggcgaactgc ctgaagaacg acaacatcgt ctatatcggc gacctgatcc agaagaccga
      841 ggcggagatg ctccgtacac cgaacttcgg acgcaagtcg ctcaacgaga tcaaggaagt
      901 tctcgcccag atgggcctgc atctcggcat ggaagtgccg aactggccgc ccgagaacat
      961 cgaagaactc gcgaagaagt acgaaacaca ttactgagac agagagggcg cccgagggtg
     1021 cccgtagtga aggagaacgg tcatgcgtca tggtttcagg ggccgccggt ttaaccgcac
     1081 cgccgagcat cgcactgcga tgtttgccaa catgtcggcg gcgttgatca agcatgagca
     1141 gatcgtcacc acgcttccca aggccaagga tctgcgcccc gtcgtggaga agctggtcac
     1201 cctggccaag aagggcagcc tgcatgcgcg ccgtcaggcc atggccgaag tgcgggacga
     1261 gacccaggtc aagaagctct tcgaggttct ggcggcccgt tacaaggatc gccacggcgg
     1321 ctatctccgc atcatgaagg ccggcttccg ctacggtgat tcagcgccca tggccgtgat
     1381 cgagttcgtc gaccgcgacg tgaatgcccg cggccaggat tccggcccgt cgaacaagcc
     1441 ggaagaggcc gccgaggcgg ctgcctaaga acaagaagtc attcggtttt cagcggggtg
     1501 gcagaaatgc cgccccgttt ccgtttgtgc tgctgacgga acgtaagggc ctgcgctccg
     1561 caacagggaa cttcgtttct caagaggcgg ctgacctgct actaaatggt taatgacccc
     1621 gcgagacgac atgattccgc gctttgacct cttcaacgag gcgcgccagc cctttgcctc
     1681 ggcgcgggaa tcctctgagt atctcaagcg ggcgcgcgac cgcttcagcg tacagaatct
     1741 gtcctactgg ttccttggct cgtcgccgca gatgccggac cgcctgacct ggctgtcgac
     1801 ctacgaccag gaatacatgg ccgtctacat gcgggagtat tcgccgttga acgatccggt
     1861 ctttgacgtg tgcttccggc gcctgctgcc gctcgactgg cacgagatgc gcgatgcgga
     1921 tcagcaggcg cagatgattc accaggtggc ggaacaatac ggcatagggc ggcacggcat
     1981 ctcgtttccg atccgtgatc ccggtttcgg cgatgcgatg ttcagcgtga acttcgaatg
     2041 cgacgatcgc cagtgggaca gtctgcgcca cgacctcgtg aacggcttcc acctgtttgc
     2101 gcatttcttc cacctgcgga tgaaggatgt catctcgcgg cgtccggtga catccgagtt
     2161 cgacctcagc ccgcgcgagc gcgaggtgct ggcctgggcg gcggatggca agacggcctg
     2221 ggagaccgct cagctgctgg gcgtcagcga gcgggcgatc cgtctgtaca cggagaatgc
     2281 catgaacaag ctgcgcgcca agaccaagac ccaggccgtg gccattgccg tgaagaacga
     2341 catcatccac tgatcaggtg gcgagcgcgg tctccacggg aatcacgcga tggccgccat
     2401 agccgttgaa catcgtggag gtggcgatgc cgtaggctcc cagcgttccg aattcgatga
     2461 agtcctcgtc catgatgtga gccggcagat cgagccggtg cggcaggacg tcgaggggat
     2521 cgcaggtcgg gccgaagacc ttccagggct tggcggggcc ggcaaggacc tcgccatcgc
     2581 ggatcacgcg atgcggcggc tgcaactcgg ggacctgcat gaattccatc aggccgccat
     2641 agacgccgtc gttgatgaag atgtcgtcgc catcgctgca gaccagcttg atgcgggtga
     2701 ggagcgacat ggcattggcg accaggccgc ggcccggttc gcattccagc tgcggctcgc
     2761 ggccggcgcc gaagcagcgc gttacggcct gtccaatggt gtggaagaag atttcgagcg
     2821 ggccgccctt gctgttggga tagtcggcgg gaaatccgcc gccgacgttg agcttcgaca
     2881 gcgtgatccc tgcagcggcg gcgatccgcg cggcggcctc gatgtggcgg acataggttt
     2941 ggggatcgcg ggtctgtgac ccgggatgga aggtgaggag gggacggtaa ccgcggcggg
     3001 cagcctcctc gagcaatgcg acggcgacat gttccggcgc accgaacttg gtcgaaaagt
     3061 catgggccga ggatccgcgg tcgcggggga gcacaaagcg cactgcgatt tcgaggctgg
     3121 cgtcgcttcc ggcgatgtcg gcgatcttgg cgagctcctc gcggcagtcc accgcgaagc
     3181 ggcggcatcc gtagcggcca taggcgtccg cgatctcgcg gcgcgacttc accgggttat
     3241 gatagtggaa cacgggcttc ggcgcctggg cgcgaaccgc ttccatctcg tggacagaag
     3301 caacgtccca gtgcttgagc ccggccttgg ccaggcaggc aatcacatcc ggcgacggat
     3361 ttgacttaac ggcaaagctt gtcgtccccg ggaacccctt ctggaaggtc ttcaggcggg
     3421 ccagcagctg cggcgcgctg aagaggaaca ccggttcctc gggctgcaga atccgggcca
     3481 cggcccgggc atccgcaaat cttccagcct tttcaagttt ccgcaccgtt gccgtcatgt
     3541 gaccctcctt ggccgaacag agccgttacc taggctcggc agcagttgga atacaggcgg
     3601 cattatgctg aaatgtgacg gtcgagtcgt ccgaacgaca agcgaggttt catttcgacg
     3661 gatggtgcag attacgccca aggacgaaca gctgatcgcg ctcctgcgtg tggacgcgcg
     3721 cgagccggtg gcatcgctgg cgcgcaagct cggcctgtcg cgcaccaccg tgcaggaccg
     3781 cctgcgccgc ctcgaaggct ccggcatcat cgccggctat tcgctcaagc tcgcccgcga
     3841 gatcgatgcc ggcggcatgc gcgccttcgt gaccctgtca atcgaaccgc ggcggcaggt
     3901 cgaggtggcc cggacgctcg cgaagtttca tcagatcgaa acgctgcaca cggtcagcgg
     3961 caagtatgat cttgtcgcgc aggtcaagac cgggacctcc gaagacatgg accggctgat
     4021 cgacgagatc ggcctcatcc ccggaatcac ccggatcgag actgcggtca tcttgtcgac
     4081 aaagctcgat cgcagatagg gcaacagact gcccaatgag gaacccatgc gcctgatcca
     4141 tttggccctt gccgccgtca tcgcacttgc cgtcccgcct gccgtgaggg cggaaaccca
     4201 ggttccgcgg tcgcaaggcg agatgctctt gtcctttgcg cccgtcgtga agcgcacggc
     4261 gcccgctgtc gtcaacgtct atgcgcagcg gattgtcgcc agccggcagg aaggcatcgg
     4321 ggcggatcca ttctttcgcc gcttcttcgg cgagaacggc ggcttcgggc gctcgcgcga
     4381 gcgggtgcag aactcgctgg gatcaggcgt gatcgtcgat ccgtcgggca tcatcgtgac
     4441 caacaatcac gtcattcgcg gcgggtccga gatcaaggtc gttctggccg accgccgcga
     4501 gttcgaggcc agcgtcgtcc tgacagatga gcgcaccgac cttgcggtgc ttcggatcga
     4561 cgtgagcgac gagga
//
LOCUS       DBNGVR010000131         4449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4170465, whole genome shotgun sequence.
ACCESSION   DBNGVR010000131 DBNGVR010000000
VERSION     DBNGVR010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4170465"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16A0_02845"
     CDS             <1..333
                     /locus_tag="AC16A0_02845"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3104299.1"
                     /translation="TLDTDLNGRPDAFNPAELFLASIAACMIKGIERVSPIIHFSLRH
                     VDIILHGVRQDAPPMMITVDYEIIVDTDEDDRRLELLHTNVRKYGTISNTVAKAVELR
                     GKIRRADN"
     gene            393..1436
                     /locus_tag="AC16A0_02850"
     CDS             393..1436
                     /locus_tag="AC16A0_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3104300.1"
                     /translation="MRIGINGMGRIGRLALRASLGGAHRGDDDPRRGNRLDVVHVNEI
                     KGGAEATAHLLEFDSVQGRWRTDISQPGETAIAVSGNIIGFSSHARAEDIPWGDLGVD
                     LVLECTGKFLTHATLDGHLKRGAKRVIVAAPVKETDVLNVVVGVNHHLYDPARHPIVT
                     AASCTTNCLAPVVKVVHEALGIRHGQITTIHDPTNTNVVVDAPHKDLRRARSAMLSLQ
                     PTTTGSATAIALIYPELKGKLDGHAVRAPVLNASLTDCVFELNRATTRDEVNDLFRAA
                     AGGPLAGILGYEPRPLVSADYARDTRSSIVDGLSTLVTDGTLLKVYAWYDNEMGYACR
                     MVDLACHMRDAGI"
     gene            1442..2680
                     /gene="arsJ"
                     /locus_tag="AC16A0_02855"
     CDS             1442..2680
                     /gene="arsJ"
                     /locus_tag="AC16A0_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HIR3104301.1"
                     /translation="MTPGTRNYAIVTAAYWGFTLTDGALRMLVLLHFFQLGYSPFTLS
                     FLFLLYEAAGIAANFIGGWLATRFGITRMLTVGLSTQIVGFLVLSALSPSWAAGLSVA
                     WVVLAQGICGVAKDLTKTASKSAIKIAEGEARSEDTESRLFRWVAWFTGSKNAMKGLG
                     FFLGGVLLEFLGFRMSLWVMAAALALILAGVVLSLPAMMGKSKASASARELLGKSRGV
                     NLLAAARVMLFGARDVWFVVGVPVFLYSTGWTFSMVGGFMALWTIFYGLVQAAAPALV
                     ARSPDGLSKEVRAAQLWSAALALIPAAIIVALQSGLGLAPDLVLVFGLAVFGIVFAVN
                     SSVHSYLILAYAGSEKAAEDVGFYYAANALGRFGGTLFSGLLYQWGGITACLAGSAAM
                     LIACFLFTLPLPSTAARAKA"
     gene            2836..3315
                     /locus_tag="AC16A0_02860"
     CDS             2836..3315
                     /locus_tag="AC16A0_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104302.1"
                     /translation="MRHLLKFLHTIGAIGLMGSMASLLVLLSLAPPPDALAEYALIRG
                     AMGSIATWIFFPSLGLTLIAGLIALGYSKAYHNAGWAWAKAISGILVFESGFVGILGP
                     MQREAERSADALAGKIEPSTLAASLTAERNTLWILLAVATANVIFGIWRPRLTKLRD"
     gene            3562..4380
                     /locus_tag="AC16A0_02865"
     CDS             3562..4380
                     /locus_tag="AC16A0_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3104303.1"
                     /translation="MPFHQGLSSRIHYEQTGTGPDIVWVGGGGTMGRDWQRFQTPHFD
                     RAFRSTVFDNRGIGQTACSEPLPWTIEDFANDLAELTRSVCRGPARFIGTSLGSAIVQ
                     QLAIDHPDLVRCAIVMGTGAWSTGWGWDYQLAEIEFRNAGGRLDGLMGVTHYASMLYP
                     ARALGDRTLWPRLKELLLEWMDSGENEASLVPQWDASLRFDQRARLPSIRVPLHVIAF
                     NEDVQAPPQDGEELASLVPTAEYHLLQGMGHGSWYGHAHEELNPFLESILRRYP"
BASE COUNT          766 a         1488 c         1358 g          837 t
ORIGIN      
        1 acgttagata ccgacctcaa tggcaggcca gatgctttca atcccgcgga gctgttcctc
       61 gcctccatcg ccgcttgcat gatcaagggc atcgagcgcg tatccccgat aatccacttc
      121 tcgcttcgac atgtcgacat catcttgcat ggtgtcagac aggacgcgcc gcccatgatg
      181 atcacagtcg actacgagat tatcgtcgac acggacgagg acgaccgccg actggaactc
      241 ctccacacca acgtccgcaa gtacggcacg atctccaaca cggtcgccaa ggcggttgaa
      301 ctccggggca agatcagaag ggccgacaac tgaccgtcac acgcgcgaga tgcgaacaca
      361 ggaagacacc cgcagcgaag aaggattgag acatgcgcat cggaatcaac ggcatgggcc
      421 gcatcggacg gctcgccctg cgcgcaagcc tgggcggcgc gcatcgcggt gacgacgatc
      481 cgcgccgcgg caatcgcctg gacgtggtgc atgtgaacga gatcaagggt ggcgccgaag
      541 cgacggccca tcttctcgag ttcgattccg tgcagggacg ctggcgcacg gacatctcac
      601 aaccaggcga gaccgccatc gcggtttctg gaaacatcat cgggttctcg tcgcacgcga
      661 gggcggagga cattccatgg ggcgaccttg gcgtcgacct tgtattggaa tgcaccggca
      721 agttcctgac tcacgcgacg ctcgatggac atctgaagcg cggcgccaag cgtgtgatcg
      781 tggcggcacc tgtcaaggag acagatgtcc tgaacgtcgt ggtcggcgtc aaccatcacc
      841 tctatgatcc agcgcggcac ccgattgtga cggcggcctc ctgcaccacc aattgcctcg
      901 cgccggtggt gaaggtggtt cacgaggcgc tcggcatccg ccacggccag atcaccacga
      961 tccacgaccc gaccaacacc aacgtcgtcg tcgatgcgcc gcacaaggac ctgcgccgcg
     1021 cgaggtcggc catgctgtcg ctgcagccca ccaccaccgg aagcgccacg gccattgcgc
     1081 tgatctatcc ggaactgaag ggcaagctcg atggtcacgc ggtgcgcgct ccggtgctca
     1141 atgcgtcgct caccgactgc gtcttcgagc tgaaccgcgc taccacgcgc gatgaggtca
     1201 atgatctgtt ccgggctgca gccggcggtc cgctcgccgg aatcctcggg tatgagccgc
     1261 ggcctctggt ctcggccgac tatgcccgcg acacccgctc ctccatcgtc gacggccttt
     1321 ccaccctcgt caccgatggc acgttgctca aggtctatgc ctggtacgac aacgagatgg
     1381 gctatgcctg ccgcatggtc gatctcgcct gccacatgcg ggacgccggg atctgaaccc
     1441 catgacgcca gggacccgca actacgccat cgtcaccgcc gcctactggg gcttcaccct
     1501 cacggatggc gcgctgcgca tgcttgtgct tctgcacttc ttccagctcg gttattcgcc
     1561 gttcaccctg tcgttcctgt tcctgctcta tgaagcagcg ggtatcgctg cgaacttcat
     1621 cggcgggtgg ctggccaccc gcttcggcat cacccgcatg ctgacggtcg gattgtcgac
     1681 gcagatcgtc ggctttctgg ttctctcggc cctgtcgccc agctgggctg cgggtctctc
     1741 cgtggcctgg gtcgtcctgg cccagggcat ctgcggcgtc gccaaggacc tgaccaagac
     1801 ggcctcgaaa tccgccatca agatcgccga aggcgaggcc agaagcgagg acaccgaaag
     1861 ccgcctgttc cgctgggtcg cctggttcac gggatccaag aatgccatga aggggctggg
     1921 gttcttcctt ggcggcgtgc tgctggagtt cctcggcttc cgcatgtcgc tctgggtgat
     1981 ggccgcagcg ctggccctga tcctggcggg cgttgtgctg tcgcttccgg cgatgatggg
     2041 caagagcaag gccagcgcct cggcacgcga acttctcggc aagtccaggg gcgtcaatct
     2101 gctggcggcg gcgcgcgtca tgctcttcgg cgcccgggac gtctggttcg tcgtcggcgt
     2161 acccgtgttc ctctattcga caggctggac cttctccatg gtcggcggct tcatggcgct
     2221 ctggaccatc ttctacggtc tcgtgcaggc cgccgccccg gcacttgtgg cacgcagccc
     2281 tgacggcttg tcgaaggagg tccgcgcagc gcaactttgg tcagccgctc tcgccctgat
     2341 cccggctgca atcatcgtgg ccctgcaaag cgggttgggt ctggcgccgg accttgtcct
     2401 cgtcttcggc ctggccgtct tcggcatcgt ctttgccgtc aactcctccg ttcattccta
     2461 cctcatcctc gcctatgccg gatcggagaa ggcggccgag gacgtgggct tctactatgc
     2521 cgcaaatgcc ctgggccggt tcgggggcac cctgttctcc ggactgctct accagtgggg
     2581 cggcatcacg gcatgccttg cggggtcggc ggcaatgctg atcgcctgtt ttctattcac
     2641 cctgcctctt ccaagtacgg cagcgagagc gaaggcatga gccgccccat ccggcacaga
     2701 ttccgaaact ccgtttcagg ctcttgcaga ttgcgcgatc gctcgccgcc cgcttgacta
     2761 cagttgatcc cgccgttccc atgccctagc atttgtcggt gtacacacgc acaacactga
     2821 cgctgacgaa acgccgtgcg acacctcctg aaatttctcc acacgatcgg tgcgatcgga
     2881 ctgatgggct caatggcatc cctgctcgtg ctgctgagtc tggcgccgcc accagacgcg
     2941 ctggccgaat atgccctgat ccgcggcgcc atgggatcga tcgccacctg gatcttcttt
     3001 ccgtcgcttg gcctgacgct cattgccggc ctgatcgcgc ttggctacag caaggcctat
     3061 cacaatgccg gctgggcctg ggcaaaggcg atcagcggca tccttgtctt cgaaagcggc
     3121 ttcgtcggca ttcttgggcc gatgcagcgc gaagccgagc gaagcgccga cgcgctggcc
     3181 ggaaagatcg aaccttcgac attggccgca tcgctcaccg ccgagcggaa cacgctctgg
     3241 atactgctcg cggtcgcaac agccaacgtc atcttcggca tctggcggcc gcgcctgacc
     3301 aagttgcgcg actgaggtgg cggaccacgt tcacgttgca ggggcgacct cccagagcgc
     3361 ctcatctgac agacgcctgc ggtcttgaag aagatgcctg ccgcgcgttc gacatcctcc
     3421 ggcaggcaca tgggcggcag caagcgcaga accgcgacac gactggagtc gtgccgattg
     3481 accgacggaa cccagctggt ccatgagtgg aaggccgccc gggctaccgc gcggcggtga
     3541 caagatattg agggtgatgc ggtgccgttc catcaaggcc tttcgtcaag gattcactac
     3601 gagcagaccg gtaccggtcc cgacatcgtc tgggtcggcg ggggcggtac catgggccgc
     3661 gactggcaga gattccagac gccgcacttc gatcgcgcct ttcgcagcac cgtcttcgac
     3721 aaccgcggca ttggccagac ggcctgttct gagccgctcc cctggacgat agaggacttc
     3781 gccaatgacc tggccgaact gacccgcagc gtctgccgcg ggccggcccg cttcatcggc
     3841 acatcgctgg gctcggcgat cgttcaacaa ctcgccatcg atcatcccga ccttgtccgc
     3901 tgcgccatcg tcatgggcac cggcgcctgg agcactggct ggggctggga ctatcagctg
     3961 gcggagatcg agttccgcaa cgccggcggc cggctcgacg gattaatggg cgtgacgcat
     4021 tacgcgagca tgctctatcc ggcgcgcgcg ctcggcgacc ggaccctttg gccccggctg
     4081 aaggaactgc tgctcgaatg gatggactcg ggcgaaaacg aagcctcgct ggtgccgcag
     4141 tgggatgcct cgctgagatt cgatcagcgg gctcggcttc cctccatcag ggttccgctg
     4201 catgtcatcg ccttcaacga agacgtgcag gcaccaccgc aggacgggga agagctcgca
     4261 agcctggtac cgacggccga gtaccacctg ctgcagggca tggggcacgg ctcctggtat
     4321 ggccacgcgc atgaggaact gaacccgttt ctggagagca tcctgcggcg atacccttag
     4381 gctgcaccgg tctcgtggaa caggtctgca cgccaggccc tgcctgtcgc catgcccccg
     4441 ccccttcct
//
LOCUS       DBNGVR010000132         1946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4170792, whole genome shotgun sequence.
ACCESSION   DBNGVR010000132 DBNGVR010000000
VERSION     DBNGVR010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4170792"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..697
                     /gene="efp"
                     /locus_tag="AC16A0_02870"
     CDS             125..697
                     /gene="efp"
                     /locus_tag="AC16A0_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3104304.1"
                     /translation="MVKVIASGVRKGNVIEHEDGKLYVVLKAESMHPGKGTPTTTIDM
                     RRISDGVKTTVRYKTTDSMERAFVETITHSYLYQDGDNYIFMNPENFEQIQVSGDMLG
                     DSAPFLQEGMECQIALFNGTPISIELPQRVTLEIVETEPAIKNQTASSSFKPAVLSNG
                     VRTMVPPHIVSGTRIVVATEDGSYVERAKD"
     gene            complement(698..1915)
                     /locus_tag="AC16A0_02875"
     CDS             complement(698..1915)
                     /locus_tag="AC16A0_02875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR3104305.1"
                     /translation="MTDATVSITAMGRRAEGLAMHEGHPLFVPLSLPGEELRVAIEGE
                     RGSIRDILAASPQRVAPFCPHYGRCGGCQLQHWQEVPYRVWKAGLVDSALAAKGIDKN
                     VSGLIDAHGAGRRRVSIHARRRDGVATAGFMAARSHDLLDLDRCPVLVPALAPAFDVA
                     RTLAARLGDSDVALTATASGLDVSVKAERKIVTAEMPKLAGLARELDLARLSVNGEVV
                     VMPRVPLVQMGKAMVPIPPVSFLQATAAGEDVLGTLVCDGLGKAKSVADLFSGCGPFT
                     FRIAERARVRAFDSDRPAIAALIAAQRTASGLKPVSAEARDLFRAPLVASELKDYDTV
                     VFDPPRAGAEAQAKQLARSQVRTVIAVSCDPVTLARDAAILVGGGYALEAVTAVDQFK
                     WTAHVETVAIFRR"
BASE COUNT          365 a          646 c          641 g          294 t
ORIGIN      
        1 ggatcgaacc agggtgtctc ggacggcggc aagggcttct cccgaggggg ttggctttgg
       61 cgggggccgc tgctaatacc cccgccagcc aattccagca attcccagaa agacgagaaa
      121 caacgtggtc aaggttatcg caagcggtgt ccgcaagggc aatgtcatcg agcatgaaga
      181 cggcaagctc tatgtcgttc tgaaagccga atccatgcat ccgggcaagg gcacgcccac
      241 cacaacgatc gacatgcgcc gcatctcgga cggcgtgaag accacggtgc gctacaagac
      301 gaccgactcg atggagcgcg ccttcgtcga gaccattacc catagctatc tttaccagga
      361 tggcgacaac tacattttca tgaatcccga gaatttcgag cagatccagg tctcgggcga
      421 catgctgggc gactccgccc ccttcctgca ggaaggcatg gagtgccaga tcgcgctgtt
      481 caacggcacg ccgatctcca tcgaactgcc gcagcgcgtg acgctggaaa tcgtcgagac
      541 cgagcccgcc atcaaaaacc agacggcgtc ctcctccttc aagccggcag tgctgtcgaa
      601 tggcgtgcgc accatggtgc cgccgcacat cgtgtcgggc acccgcatcg tggtggcgac
      661 cgaggacggt tcctacgtgg aacgcgccaa ggactgatca cctgcggaag attgcgacgg
      721 tctcgacatg ggccgtccac ttgaactggt cgacggccgt gacggcctcc agcgcatagc
      781 cgccgccgac aaggatggcg gcatcgcgcg ccagcgtcac cggatcgcag gagaccgcga
      841 tcaccgtcct gacctgcgac ctggcgagct gcttcgcctg cgcttcggcc ccggcgcggg
      901 gcgggtcgaa gaccaccgtg tcataatcct tcaattcgga ggccacgagc ggcgcgcgga
      961 acagatcgcg cgcctcggcc gagacgggct tgagccctga cgcggtgcgc tgggcggcga
     1021 tgagcgcagc aatcgccggc cgatcactgt cgaaggcgcg gacccgggca cgttcggcga
     1081 tgcggaaggt gaaggggccg cagccgctga agagatcggc aaccgatttg gccttgccca
     1141 gcccgtcgca caccaaggtg ccgaggacgt cctcacctgc ggccgtggcc tgcaggaagg
     1201 agactggcgg aatgggcacc atggccttgc ccatctgcac cagcggcacc cgcggcatga
     1261 ccaccacctc gccgttgacg ctgagccgcg ccagatcgag ttcgcgcgcg aggcctgcaa
     1321 gcttcggcat ttcggcggtg acgatcttgc gctccgcctt gacgctgacg tcgaggccgc
     1381 tggctgtggc ggtgagggcg acgtcgctgt cgcccagcct ggcggcgagg gtccgcgcga
     1441 catcgaaagc cggcgcaagc gcaggaacca gaaccgggca gcggtcgagg tcgagcaggt
     1501 catggctgcg cgccgccatg aagcccgccg tcgcaacccc gtcgcgccgc cgcgcatgaa
     1561 tgctgacacg ccggcgcccg gcgccatgcg catcgatcag tccactgacg ttcttgtcga
     1621 tccccttcgc cgcaagcgcg gaatcgacaa gtccggcctt ccagacgcga tagggaactt
     1681 cctgccagtg ctgcagctgg caaccgccgc agcggccgta atgcggacaa aagggtgcga
     1741 cccgctgcgg cgaggcggcg aggatgtcgc ggatgctgcc gcgctcgcct tcgatggcga
     1801 cccgcagctc ctcgcccgga aggctcagcg gcacgaagag cggatggccc tcatgcatcg
     1861 caaggccttc cgcgcggcgg cccatggccg tgatcgagac ggtggcgtca gtcatggctg
     1921 tcgaaccgcg gcatggcgat ggcgac
//
LOCUS       DBNGVR010000133         5246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1226575, whole genome shotgun sequence.
ACCESSION   DBNGVR010000133 DBNGVR010000000
VERSION     DBNGVR010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1226575"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="AC16A0_02880"
     CDS             complement(<1..317)
                     /locus_tag="AC16A0_02880"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR3104306.1"
                     /translation="MQIATWNVNSIKARTDAALAWLKEASPDVVCLQEIKCEDSAFPS
                     ALFEDLGYNVVTHGQKTYNGVALLSKMRLEDVRMGLPGDSDDTQARYIEAVVHGAEGA
                     FRIG"
     gene            complement(355..681)
                     /locus_tag="AC16A0_02885"
     CDS             complement(355..681)
                     /locus_tag="AC16A0_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR3104307.1"
                     /translation="MTAITLTERAAQRIKEITAAEAGKSALRVAVNGGGCSGFQYEFL
                     LESGPAADDLVIERSGAVAVIDSVSQGFLDGSVIDFVDDLIGQSFKIMNPNATSSCGC
                     GTSFSI"
     gene            790..1929
                     /locus_tag="AC16A0_02890"
     CDS             790..1929
                     /locus_tag="AC16A0_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR3104308.1"
                     /translation="MTEPDSPPRTPFARDRDRIIHSSAFRRLKHKTQVFVYHEGDHYR
                     TRLTHSIEVAQVARSIARALGLDEDLTEALALAHDLGHTPFGHAGERELDRLMKPHGG
                     FDHNAQSLRIVTRLERRYADFDGLNLTWETLEGLVKHNGPLLDAKGNPHGPYREQGLP
                     EAIIEYAEVHDLKLALYPSAEAQVAAMSDDIAYNAHDIDDGLRAGLFGVIDLGDVPLA
                     GEALSDVLRRYPGIDTQRTIHETVRRVISVMIADVIAETRNRAQAAGVTSADEVRHLG
                     KPLVGFSLQMAEKNRTLQSFLSGKMYRHPQVTEIMGRAQRVVRDLFEAYQGDPGLLPP
                     NWREGSFTDDRSRFARQVCDFIAGMTDRFALDQHKRLFDLDPLFR"
     gene            1970..3703
                     /gene="argS"
                     /locus_tag="AC16A0_02895"
     CDS             1970..3703
                     /gene="argS"
                     /locus_tag="AC16A0_02895"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3104309.1"
                     /translation="MNLFSDFLASIRTAANAVAADLALQGTIDLDKVTAEPPRDPAHG
                     DIATNAAMVLTKAFGKPPRVLAEAIAAKLREHTDVAHVAIAGPGFINIRLRHTVWPRL
                     LDAALSHGGSYGGSRLGGGLKANVEYVSANPTGPLHVGHCRGAVFGDALAQLLTETGF
                     DVTREYYINDAGAQVDTVARSAFLRYREALGETIGEIPPGLYPGDYLKPVGVALAREH
                     GDGLLALDEAQWLPLVRAKAMAMMMDLIRNDLAALNIRQEVFFSERSLHDSGEVADTI
                     ADLRAAGHVYQGSLPPPKGEVPDDWEDREQTLFRATAFGDDMDRPLLKSDGSFTYFAS
                     DVAYSRNKIRRGYKELIYILGADHGGYVKRLEAVAAAVAGPGAVKVVVRLCQLVKLYR
                     AGEPVRMSKRAGEFVTLRDVVDEVGPDVVRFMMLFRKNEAPLDFDFAKVTEQSKDNPV
                     FYVQYAHARTCSVLRNAEAEGLSGGKPDFALLTDPAEMGIIRRIAEYPRLLESAAIAH
                     EPHRVAFYLYELAGEFHALWNKGKESPQLRFILREDLETTITRLAFLRVIRYVLANGL
                     RILGVRPADEM"
     gene            3660..>5246
                     /locus_tag="AC16A0_02900"
     CDS             3660..>5246
                     /locus_tag="AC16A0_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104310.1"
                     /translation="MGCGFSACDLPTRCRSETADMDQDSTNGTSDKRNWRERLGIGTR
                     DMPKISDEFKAQPGAESKTEQEQPIARPAVKPPQPVSRTAPMAPRNPAPMAPRAAPAK
                     QPAATNPAQAAARPAPPNEALADKLRAQRAAAERLAEQRVQAARERAESRMSDQPSAR
                     PKVAEPPQKPSTSARPKFSFADDANTAEVPPPVTRTPAGPLVPPRPALGGDRGQPPFL
                     RPSGPSAPTPSFRTETPPYRPIDPATGYPGASARYQPTYRGNYGNEPGLGSATGRPPP
                     PARRTPGYDPYARGQEPAFEPEPFADDTPRLGRPAAPRGRGRAAYEEDQEDVFEDEQP
                     RRRASARDYHSAYEQGDEGLEEGRRRSKGPWLLLSALLAAAVATAGVVWYYNTNIKTG
                     STASQGEVPVIAAPEEAAKTAPEAATDATGESPALKKKQIYDRIVGEQEMTGEQMVPT
                     EEVPVQPEPAAAATDEGGTSAIPAPAGAVPDALEEPAPLPLPPPESDQQGSLSQTGAE
                     KLAAAASGDPLNLPVPGTTTG"
BASE COUNT          963 a         1700 c         1662 g          921 t
ORIGIN      
        1 ccgatgcgaa acgctccttc cgcaccgtgc acgactgctt caatgtatcg cgcctgcgtg
       61 tcgtcgctgt cgccgggcag gcccatacgc acgtcctcga gcctcatctt cgagagcagt
      121 gcgaccccat tgtaggtctt ctgaccatgg gtgacgacgt tgtagccaag atcctcgaac
      181 agggcagaag gaaacgcgct gtcttcgcac ttgatttcct gcaagcacac gacgtccggg
      241 ctggcttcct tcagccaggc gagcgctgcg tcggtgcggg ccttgatcga attgacattc
      301 caggttgcaa tttgcatggg cccatgatgg cggttcaccg ccagtgccgc aagctcagat
      361 cgagaaactg gtgccgcagc cgcaggacga cgtcgcgttg gggttcatga tcttgaagga
      421 ctgcccgatc aggtcgtcga cgaaatcgat caccgaacca tccaggaaac cctgcgacac
      481 cgagtcgatc accgccactg ctccggagcg ctcgatcacc agatcatccg cggctgggcc
      541 gctctcgaga aggaactcgt actggaagcc cgaacagcca ccgccgttca cggcgacgcg
      601 gagggcagac ttgcccgctt ccgctgcggt gatttccttg atgcgctgtg ctgcgcgctc
      661 ggtgagggtt atggcggtca tgacgattgt gtatgttggg aagaacacgg cgcttgtcaa
      721 tggattcccg cgatgcagcg attaacggct ccctttgcct caagtcctga tctatcgcgc
      781 gggcggctgg tgacggagcc ggactcgccg ccgcgcacgc ccttcgcgcg cgaccgggac
      841 cggatcatcc attcctcggc ctttcgccgc ctcaagcaca agacgcaggt cttcgtctat
      901 cacgagggag atcactaccg gacgcggctg acacactcga tcgaggtggc gcaagtggcc
      961 cgctccatcg cgcgcgcgct cggtcttgac gaggacctga ccgaggccct ggcccttgcg
     1021 catgatctcg gccacacgcc cttcggccat gctggcgagc gcgaactcga ccgcctcatg
     1081 aagcctcatg gcggcttcga tcacaatgcg cagtcgctac gtatcgtcac ccgcctggag
     1141 cgccgctatg cggacttcga cggtctcaat ctgacctggg aaacgctcga gggcctggtc
     1201 aagcacaacg ggccgctgct cgacgccaaa ggcaatccgc atggtcccta ccgcgaacag
     1261 ggattgcccg aggccattat cgagtatgcc gaggttcacg acctgaagct ggcgctctat
     1321 ccttccgccg aagcacaggt cgcagcgatg tcggacgaca ttgcctacaa cgcccatgac
     1381 atcgacgatg gcctgcgggc ggggctcttc ggcgtgatcg atcttggcga tgtgccactg
     1441 gcgggcgaag ctctctccga cgtgctgcgc cgctatccgg gtatcgacac ccagcgcacc
     1501 atccacgaaa ctgttcgccg tgtgatttcc gtcatgattg ccgacgtgat cgcggaaacc
     1561 cgcaaccggg ctcaggctgc gggcgttacg tcggcagacg aggttcggca tctgggcaaa
     1621 ccgctggtcg ggttcagcct ccagatggca gagaaaaacc ggaccctgca atcattcctc
     1681 tccggcaaga tgtacaggca tccccaggtc accgagatca tgggccgggc acagcgtgtg
     1741 gtgcgcgatc tcttcgaggc ctaccagggc gatcccggcc tgttgccccc caactggcgg
     1801 gaaggttcct tcaccgatga ccgaagccgc tttgcccgtc aggtctgtga cttcatcgcc
     1861 ggcatgacgg accgtttcgc gctggatcag cacaaacgtc tgtttgacct cgatccgctt
     1921 ttccgctagg gcgaccgttc gcattgaatt caaaggctta gactgctcaa tgaatctgtt
     1981 ttctgatttc ctcgccagca tccgcaccgc ggccaatgcg gtggctgcgg atcttgcctt
     2041 gcaagggacg atcgacctcg acaaggtcac ggcagaaccc ccgcgcgatc ccgcacacgg
     2101 cgacatcgcg accaacgcgg cgatggttct gaccaaggcc tttggcaagc cgccgcgggt
     2161 tctggcggag gcgatcgctg caaagcttcg cgagcacacg gatgtcgccc atgtcgctat
     2221 cgctggcccc ggctttatca acatccggct gcgccacacg gtctggccgc gtctgctgga
     2281 cgcagcattg tcgcacggcg ggtcctatgg cgggtcccgg ctgggcggcg ggctcaaggc
     2341 caacgtggag tatgtgtcgg ccaatccgac ggggccgctg cacgtcgggc actgccgtgg
     2401 cgccgtcttc ggcgacgcgc tggcgcagtt gctgacggaa accggcttcg acgtcacgcg
     2461 ggagtactat atcaacgacg cgggagcgca ggttgatacc gtcgcgcgct cggcctttct
     2521 gcgctatcgc gaggctctgg gcgaaaccat cggcgaaatc ccgccgggac tctatccggg
     2581 cgattacctg aagccggtcg gcgtggccct ggcgagggag cacggtgacg gtttgctggc
     2641 gctcgacgag gcacagtggc taccgctcgt ccgtgccaag gccatggcca tgatgatgga
     2701 cctgatccgg aacgatctgg ccgccctgaa cattcgtcag gaggtgtttt tctccgaaag
     2761 gtctctgcat gacagcggcg aggtggcaga tacgattgcc gatctgcgcg ccgccggaca
     2821 cgtctaccag gggagccttc cgccacccaa gggcgaagtg cctgacgatt gggaagatcg
     2881 cgagcagaca ctgttccgcg ccaccgcgtt cggagacgac atggaccggc cgctgctgaa
     2941 gtctgacggg tcctttacct atttcgcctc tgacgtcgcc tatagccgca acaagatccg
     3001 tcgcggctac aaggaactga tctacatcct cggggctgat catggtggct atgtcaagcg
     3061 gctggaagcc gtcgccgcag cggtggcggg ccccggggcg gtcaaggtcg tggtccggct
     3121 gtgccaactc gtgaagctct accgcgcggg cgaaccggtg cggatgtcga agcgcgccgg
     3181 cgagttcgtg acattgcgcg acgtggtgga cgaggtgggg ccggacgttg ttcgcttcat
     3241 gatgctgttc cgcaagaatg aggcgcctct cgacttcgac ttcgccaagg tcacggaaca
     3301 atccaaggac aatccggttt tctatgttca gtacgcccat gcccggacct gttcggtgct
     3361 ccgcaatgcg gaggcggagg gattgagcgg gggcaaaccg gatttcgcct tgcttacgga
     3421 tccggccgaa atgggcatca ttcggcggat cgcggaatat ccacgtcttc ttgagtccgc
     3481 ggccattgcc catgagcccc atagagtggc attctatctc tatgagctgg ctggcgaatt
     3541 ccacgcgctc tggaacaaag gcaaagagtc gcctcaattg agatttattt tgagggaaga
     3601 tctggagaca acgataacgc ggctcgcatt cttgcgcgtg attcgctatg ttttggcgaa
     3661 tgggctgcgg attctcggcg tgcgacctgc cgacgagatg tagaagcgaa acggctgaca
     3721 tggatcagga ttcgacaaac ggcaccagcg acaaacgcaa ctggcgggaa cgcctcggga
     3781 ttggcacacg ggacatgccc aagatttctg acgaattcaa agctcagccg ggcgctgagt
     3841 caaaaaccga acaggaacaa ccaattgcgc gtccggcagt caagccgccc cagcctgtga
     3901 gccgtactgc gccgatggcg ccgcgcaacc ctgcgcccat ggcgccgcgt gcggccccgg
     3961 caaagcagcc tgcggcaacc aatccggctc aagcggcagc acgcccggcg ccgccgaacg
     4021 aagccctggc tgacaagctg cgggcccagc gggctgccgc cgagcggttg gccgagcagc
     4081 gggtccaggc tgcgcgggag cgcgccgaaa gccgcatgtc cgaccagcct tcggcacgcc
     4141 ccaaggtggc cgagccgccg cagaagccgt cgacatccgc caggccgaaa ttcagctttg
     4201 ccgatgatgc caacactgcc gaagtcccgc ctccggtgac ccggactccg gcaggtccgc
     4261 tcgttccgcc gcgtccggct ttgggcggcg accgtggcca accgccgttc ttgcgcccgt
     4321 ccgggccttc ggcacccact ccgtcgttcc gcaccgagac gccgccctat cggccgattg
     4381 atccggccac cggctatccc ggagcgtccg cgcgctatca gcccacctat cgcggcaact
     4441 atggcaacga acctggcctc ggcagtgcga caggtcgacc gccgccgccc gcccggcgga
     4501 cacccggata tgatccctat gcccgcggcc aggagcctgc tttcgagccg gagccctttg
     4561 cggacgacac gccgcgcctg ggccgtccgg ccgcaccgcg cggtcggggc agggcagcct
     4621 atgaagaaga ccaggaagat gtcttcgagg atgaacagcc gcgccgccgg gccagtgccc
     4681 gggattatca cagcgcctat gagcagggtg acgaggggct cgaggagggg cgccggcgtt
     4741 ccaaggggcc ctggctcctg ctgagtgctt tgctggcggc cgctgtggcg acggccggtg
     4801 tcgtctggta ctacaacacg aacatcaaga ccggctcgac agcgtcgcag ggcgaggttc
     4861 ctgtgattgc tgcgcctgaa gaagcagcca agaccgcccc cgaggcggcg acagatgcca
     4921 cgggtgaaag cccggcgctg aagaagaagc agatctacga ccgcatcgtc ggcgagcagg
     4981 aaatgacggg cgagcagatg gtgccgaccg aggaggtgcc cgttcagccg gaaccagccg
     5041 ccgccgcgac cgatgaaggc ggcacgagcg ccattccggc gcccgcgggc gcagtacccg
     5101 atgccttaga ggagcctgcg cccctgccat tgccgccgcc ggaaagcgac cagcagggca
     5161 gtctgagcca gaccggtgcc gagaagctgg cggctgctgc gtccggcgat cctctgaacc
     5221 ttccggtgcc tggcaccacc accggt
//
LOCUS       DBNGVR010000134         4360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5351167, whole genome shotgun sequence.
ACCESSION   DBNGVR010000134 DBNGVR010000000
VERSION     DBNGVR010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5351167"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC16A0_02905"
     CDS             complement(<1..395)
                     /locus_tag="AC16A0_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR3104311.1"
                     /translation="MSNLRIHPRNSEGHIVRVTPDSAGWTYVGFDLWKLKPGMRAVGS
                     ETGRETCLVLISGRADVSAGREDFGILGQRMSPFEGKPWSVYVSGSDSWSVTAETDVT
                     LAVCTAPGRGTIASRVIGPGGLHQETRGKG"
     gene            complement(415..2343)
                     /locus_tag="AC16A0_02910"
     CDS             complement(415..2343)
                     /locus_tag="AC16A0_02910"
                     /EC_number="2.7.1.92"
                     /EC_number="4.1.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     5-dehydro-2-deoxygluconokinase/5-dehydro-2-
                     deoxyphosphogluconate aldolase"
                     /protein_id="HIR3104312.1"
                     /translation="MTSAQETAPKLDVVTIGRCSVDLYGQQIGSRLEDIASFAKSVGG
                     CPANIAIGTARLGLKSAIITRVGDEQMGRFVKEQMAREGVALDGIATDPARLTALVLL
                     SVENDRTFPLIFYRDNCADSALDDSDIDEDLIASSASVLVTGTHFSKPHSDAAQRKAM
                     RLARKHGGKVILDIDYRPNLWGLAGLGAGEERYIRSDRVSAHLKTILPDCDLIVGTEE
                     EVHIAAGSEDTLEALKIIRALSKAVIVLKRGPMGCVVYPDAIPDALDKGIVGQGFPIE
                     VYNVLGAGDSFLSGFLRGWLRGEDHATSATWANACGAFAVSRLLCSPEIPTWEELQYF
                     LAHGSPHRALRKDEAINHVHWATTRRPQPGVLMALAIDHRKQLADMANRLGAPAERIN
                     GFKRLAMQAIAKVAVGRPGFGTLLDDIYGREAMFDAARLGLWVARPLEEPGSRPLRFE
                     FSQDVGARLVDWPVTHTVKCLSFYHPDDPAALRAEQTQKIRTLYEAARKVGRELMVEI
                     IAGKHGAMDDSTIARALADHYALGIKPDWWKLEPQATPTAWRNIGDVIRRHDPWCRGI
                     VLLGLEAPEAELARGFDTAAGEPLVKGFAVGRTIFNAAAEGWLAGRITDEQAVADMAQ
                     RFASLVETWNGVQRRRAV"
     gene            complement(2435..3274)
                     /locus_tag="AC16A0_02915"
     CDS             complement(2435..3274)
                     /locus_tag="AC16A0_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR3104313.1"
                     /translation="MTSADGPPRDFAGLKSLIAERAGGLPRRLTQIASFALEHPDEIA
                     FGTVSSIAEQAEVQPSTLVRLSQALGYQGFSEMQEVFRARLRDRVLNYDERIAQLRAR
                     AAASSKPNAVFHGFSDAAEQSIASLRERLDPRQLDKAVDMLSRAQTLYLVGLRRSFPI
                     ASYMAYAYGKLGVRAILIDAVGGMAAEQMSFATPKDTVLAVSFTPYASETVALAQSVA
                     ARQVPLVSITDSPFSPLAQIADLWIEVVEANFEGFRSMSAALVLAMTLTVAVAERRGK
                     EQK"
     gene            complement(3365..>4360)
                     /locus_tag="AC16A0_02920"
     CDS             complement(3365..>4360)
                     /locus_tag="AC16A0_02920"
                     /inference="COORDINATES: protein motif:HMM:NF022449.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR3104314.1"
                     /translation="ERSEAARRALLAAEVNDAAKRKGALLIPSFAVERTQEIVTDLVA
                     LMEQDAVPRANIFIDSPLASKATRIFTKHARELQNGDALARAFNSDIVRSTESVEDSK
                     ALARFTGFRIIVAASGMCEAGRIRHHLKNHLWQPSTTVLLAGFQAQGSLGSILESGAK
                     TVTIMGETISVKAAIRRIEDYSGHADGPELVQWVKERLPISRSIFLTHGEEQAQIDLE
                     ADLHTLDIARDCIVRPRLDDVYELSGTACAFLAAETHPRIDPAMVARLDSSNEMADLV
                     LDIQEAVSRAADEKAKAVLLRRIRRALSADGSGGGDRRRPPISGVSRRSRGWDEG"
BASE COUNT          718 a         1429 c         1460 g          753 t
ORIGIN      
        1 cccttgccgc gggtctcctg gtgcagtccg ccggggccga tgacgcgcga ggcaatcgtg
       61 ccgcgtccag gcgcagtgca gaccgccagc gtcacatcgg tctcggccgt gacgctccag
      121 gagtcgctcc cggaaacata gaccgaccac ggcttgcctt cgaagggcga catgcgctgg
      181 cccagaatgc cgaagtcctc acgtccggcc gagacatcgg cgcgccccga gataaggacg
      241 aggcaggtct cgcgtccggt ctcgctgccc accgcccgca tgccgggttt cagcttccac
      301 aggtcgaagc ccacataggt ccagccggcg gagtcgggcg tgacgcggac gatgtggcct
      361 tcgctgttcc gcggatgaat cctaaggttc gacatgatgg gctccttcac tggatcagac
      421 ggcgcgcctg cgctgcacgc cattccaggt ttctaccaga cttgcgaagc gctgcgccat
      481 gtccgcgacc gcctgctcgt ccgtgatgcg cccggccagc cagccttcgg ccgccgcgtt
      541 gaagatggtg cggcccaccg cgaagccctt gaccagcggc tcccccgccg ccgtatcgaa
      601 gcccctggcc agttcggcct ccggcgcctc gagcccgagc agcacgatgc cccggcacca
      661 gggatcgtgc cggcggatga cgtcgccgat gttccgccat gccgtcggcg ttgcctgcgg
      721 ctcgagcttc caccagtcag gcttgatgcc cagggcatag tgatcggcca gggcgcgggc
      781 gatcgtgctg tcgtccattg cgccatgctt gccggcgatg atctccacca tcagttcgcg
      841 gccaaccttg cgggcggctt cgtaaagcgt tcggatcttc tgcgtctgtt cggcgcggag
      901 tgcggcgggg tcgtcggggt gatagaagga caggcatttc accgtgtggg tgaccggcca
      961 atcgacgagg cgcgcgccga catcctggct gaattcgaag cgcaatggcc gcgagccggg
     1021 ctcctccagg gggcgggcca cccagagacc gagccttgcc gcatcgaaca tggcctcgcg
     1081 gccatagatg tcgtcgagca aggtgccgaa gcccggccgg ccgaccgcca ccttcgcgat
     1141 ggcctgcatg gcgaggcgct tgaagccgtt gatgcgctcg gccggcgcgc cgaggcggtt
     1201 agccatgtcg gcaagctgct tgcggtggtc gatggccagt gccatcagca cgcccggctg
     1261 cggccgccgt gtcgttgccc agtggacatg gttgatcgcc tcgtccttgc gcagggccct
     1321 gtgcgggctg ccgtgggcga ggaagtactg cagctcctcc caggttggga tttcgggcga
     1381 gcagagcagg cgcgacaccg cgaaggcgcc gcaggcattg gcccaggtgg cggatgtagc
     1441 gtggtcctcg ccgcgcagcc agccgcgcag gaaccccgac aggaagctgt cgccggcgcc
     1501 cagcacattg tagacctcga tcggaaagcc ctggccgacg atgcccttgt cgagcgcatc
     1561 ggggatcgca tcaggataga cgacgcatcc catgggtccg cgctttagca cgatcaccgc
     1621 tttcgacagt gcacggatga tcttcagcgc ttcgagcgtg tcctcgctgc ctgcggcgat
     1681 gtggacctcc tcttcggtgc ccacgatcag gtcgcagtcg ggaaggatgg tcttgagatg
     1741 agccgacacc cggtcagacc tgatgtaacg ctcctcgccc gcacccagcc cggcaagccc
     1801 ccacagattg ggccggtagt cgatgtcgag gatgaccttg ccgccatgct tccgggcgag
     1861 acgcatggcc ttgcgctggg cggcatcgct atgcggcttg gagaagtgcg tgccggtcac
     1921 cagcaccgaa gccgaggagg cgatcaggtc ctcgtcgata tcggagtcgt cgagggcgga
     1981 atcggcgcaa ttgtcgcggt agaagatgag cggaaaggtc ctgtcgttct cgaccgagag
     2041 aaggaccagg gcggtgaggc gcgccggatc ggtcgcaatg ccgtcgaggg ccacgccctc
     2101 gcgcgccatc tgctccttga cgaagcggcc catctgctcg tcgcccacgc gggtgatgat
     2161 cgcggacttc agccccagcc gggccgtgcc aatggcgatg tttgcggggc agccccccac
     2221 cgacttggcg aaggaggcga tgtcttccag tcgcgagccg atctgctggc cgtaaagatc
     2281 caccgagcag cgcccgatgg tgacgacatc cagtttcggc gcggtctctt gcgcggacgt
     2341 catttccgcc tcctggttca gggttcccgc ttgtgaaaca ttaattccaa ttacaagtca
     2401 atacagaaca atcattctaa atcaattcca aagtctactt ctgttccttg cctcgccgct
     2461 cggcaacagc aacggtcaga gtcatcgcca gcaccagcgc ggccgacatg gagcggaagc
     2521 cctcgaaatt cgcctcgacc acttcgatcc agagatcggc gatctgggcg aggggcgaga
     2581 aggggctgtc ggtgatcgag accagcggca cctgccgcgc cgccacggac tgcgccagcg
     2641 caacggtctc gctggcgtag ggcgtgaagc tgacggccag taccgtatct ttcggcgtgg
     2701 cgaaggacat ctgttcggcc gccatgccgc cgaccgcatc gatcaggatg gcccgcacgc
     2761 ccagcttgcc gtaggcatag gccatgtagg atgcgatcgg aaaggaccgg cgcaggccga
     2821 cgaggtaaag cgtctgcgcc ctggacagca tgtccacggc cttgtcgagc tgccgggggt
     2881 cgagcctctc gcgcagcgat gcgatcgact gctcggcggc atcgctgaag ccgtggaaca
     2941 cggcgtttgg cttggagctg gcggccgcgc gggcgcgcag ctgggcgatg cgctcgtcat
     3001 agttgagaac gcggtcgcgc agcctggccc ggaagacctc ctgcatctcc gagaaaccct
     3061 gatagccgag ggcctgcgat aagcgcacca gagtggaggg ctgcacctca gcctgctcgg
     3121 caatgctcga gacggtgccg aaggcgatct cgtcggggtg ctccagcgcg aagctggcga
     3181 tctgggtgag acgccgcggc aggccgccgg cccgctccgc gatcaggctc tttagcccgg
     3241 cgaaatcccg tggtggcccg tcggccgagg tcatggtctg ctccatcaag gcaagcggag
     3301 gtcgatggac tgtctattcc atttctccta ccgatggaat tgaaagccgc gggcgacgcc
     3361 cgccttagcc ctcatcccag ccgcggctgc ggcgcgatac ccccgaaatt ggcggccgcc
     3421 gccggtcgcc gccgccgctg ccgtcggcag acagcgcccg ccggatgcgg cgcaggagga
     3481 cagctttcgc cttttcgtcc gccgcccggg agacggcctc ctggatgtcg aggacgaggt
     3541 cggccatttc gttgctgctg tcgaggcgcg cgaccatggc cgggtcgatg cgcggatggg
     3601 tctcggccgc caggaaggcg caggccgtgc cggaaagttc atagacatcg tcgagccgcg
     3661 gccggacgat gcagtcccgg gcgatgtcca gcgtgtggag atcggcttcc agatcgatct
     3721 gcgcctgctc ctcgccgtgc gtaaggaaga tgcttcgact gatcggcagg cgttccttca
     3781 cccattgcac cagctcaggt ccatcggcat ggccggaata gtcctcgatc ctgcggatcg
     3841 ccgccttcac gctgatggtc tcgcccatga tcgtgacggt tttggcgcca ctctcgagga
     3901 tgctgcccag cgaaccctgc gcctggaaac cggccagcag cacggttgtc gagggctgcc
     3961 agagatgatt cttcaggtga tggcggatgc ggccggcttc gcacatgccg ctggcggcaa
     4021 cgatgatgcg gaagccggtg aagcgcgcca gcgccttgga atcttcgacg ctctcggtcg
     4081 acctcacaat gtcggagttg aaggcgcgcg ccagcgcgtc gccgttctgc agctcgcgcg
     4141 catgcttggt gaagatgcgc gtggccttgc tggcgagcgg cgagtcgatg aagatgttgg
     4201 cgcgcggcac cgcatcctgt tccatcagcg ccacgagatc ggtgacgatc tcctgcgtgc
     4261 gctccactgc aaaggacgga atgagcagcg cacccttgcg cttggccgcg tcattgacct
     4321 cggcggcgag cagcgcgcgg cgtgccgcct cggaacgctc
//
LOCUS       DBNGVR010000135         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1030436, whole genome shotgun sequence.
ACCESSION   DBNGVR010000135 DBNGVR010000000
VERSION     DBNGVR010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1030436"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1390)
                     /gene="murJ"
                     /locus_tag="AC16A0_02925"
                     /pseudo
     CDS             complement(1..1390)
                     /gene="murJ"
                     /locus_tag="AC16A0_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
     gene            complement(1405..>2489)
                     /locus_tag="AC16A0_02930"
     CDS             complement(1405..>2489)
                     /locus_tag="AC16A0_02930"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332055.1"
                     /note="catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR3104315.1"
                     /translation="LGPRFGLTPAETDTVAWLIRHHLLMSETAQMRDLNDFKTVLDFS
                     SVVQSLERLKLLLILTVADIRAVGPGVWNGWKGQLLRTLYAEAEPVLSGGHTALSRRD
                     RVAEAQAAFFARMPDWSDEQKKAYAARHYDAYWLSVATGKQLRHHELIATAAPKTVVT
                     AVETDAFTSITELTIYAPDHPRLLALITGACAAASANIAGAQIFTTADGMALDTILIQ
                     REFQTEEDERRRAERVADIIRKALRGELWLKEAVARAYRPRTRINAFTVEPRVIIDNQ
                     SSNRFTVIEINGLDRLGLLYDLTEALYRLNLNIGSAHVTTFGEKAIDVFYVTDLTGGK
                     IENEPRRKQIENALTAVLRPARPAAA"
BASE COUNT          468 a          742 c          887 g          392 t
ORIGIN      
        1 ccagcagcag cgcgcccatc gcgagcgcgg ccagcacgat ccgcggcagg cgctggcgca
       61 gccgctggtc aagggcgaag tggccgcgcc gattcagggt aaatgccagc tgaaaggcat
      121 ttacccatgc cgcgatggtg gtacccatgg cgacgccgac atgcccgaaa acctgcgcca
      181 gggcaaggct cgcggcgatg ttcaccacca ccgacacgat ggcgaagcgc atcggtgtcg
      241 cggtgtcctc gcgggcatag aagcccggct ggaagacctt gatgagggca aaggccggca
      301 ggcccgctgc aaagaccagc acggcaggcg ccacggccat ggtgtcggcc cgggtgaatg
      361 cgccgtgctc gtagacagta tggatgatcg gtgcgggtat ggccatcagg gccatggccg
      421 ccggcaaggt gagaaacagc gacagttcca gcgcccggtt ctggctggca agcgcggcgg
      481 catccttgcc ggcacggagc ttgcgcgaca gatccggcag cagcaccacg ccgatggcga
      541 cgccgatgac gcccagcggc aattggtaga ggcgatcggc gtagtagagg taggagacgg
      601 cgcgatcgat cgtggtggcg atcatggtcg cgatcaccag attgatctgg gtgatgccgc
      661 cggaaatgat gccgggaatc gaaagccgga tcacccggcg cacatcgggc gtgagcttcg
      721 gcatgcgcgg caggagccgc atcccggcgc gccggcaggc gatcgccagc agcagatact
      781 gcgcgatgcc cgcagcgaag atgccccagg ccaagatata gcccgtcgcc gccgtgttgc
      841 ccgtgccgaa ctaccaggcc acgacattgg ccgcgatcat gaccaggttg agcaggatcg
      901 gtgcggctgc cgcggcggtg aagcggttga ggctgttgag gacgccggac tgcagcgcgg
      961 tcagggagat gaagagcagg tagggaaagc agatgcgggt gagatcgacg gcgaggtcga
     1021 acttcggtgg atcatcggag aagcctggcg cgatcaccag catgatcagc ggcattgcga
     1081 tcatcgcgac gaccgagaag agcatcagcc acgtcagcag gacggaaaag acttcgatgg
     1141 cgaacgcgcg tgccttggcc tcgccatcgc cttcgatttt cttggcgaaa agcggcacga
     1201 aggcggaatt gaaggcgccc tccgcaaaga gcgccctgaa gagattggga aatcgctgag
     1261 ccacgaagaa ggcctcggcg atcgggccgg tgcccagcgt gaaggcgatg agctgatcgc
     1321 gcaggaagcc gaggaggcgg ctgatcaggg tgaagccgcc gacggttgcc gcggatttga
     1381 gcagcgacat tcaggcaccg cccttcatgc cgccgccggc cgggccggcc tgaggacggc
     1441 ggtcagcgca ttctcgatct gcttgcggcg cggctcgttc tcgatcttcc cgccggtgag
     1501 atccgtcacg tagaagacgt cgatggcctt ctcgccaaag gtcgtcacgt gcgccgagcc
     1561 gatattgagg ttgagccggt acagtgcctc cgtcaggtca tagagcaacc cgagacgatc
     1621 gaggccattg atctcgatga cggtgaagcg gttggacgac tggttgtcga tgatgacgcg
     1681 cggctcgacg gtaaaggcat tgatgcgggt ccgcgggcga taggcgcggg cgaccgcctc
     1741 cttgagccag agttcgccgc gcagcgcctt gcgaatgatg tcggccacgc gttcggcacg
     1801 gcggcgctcg tcctcctcgg tctggaactc gcgctggatc agaatcgtgt cgagcgccat
     1861 gccgtcggcg gtggtgaaga tctgtgcgcc cgcgatgttg gcgctggccg ccgcacaggc
     1921 gccggtgatg agcgccagca ggcgcggatg atccggcgca tagatggtga gctcggtgat
     1981 cgaggtgaag gcgtcggttt caaccgcggt gacgaccgtc ttgggcgcgg ccgtggcgat
     2041 cagctcatgg tggcgcagct gcttcccggt ggccacgctc agccagtagg catcgtagtg
     2101 gcgcgccgca taggccttct tctgctcgtc gctccagtcc ggcatgcggg cgaagaaggc
     2161 ggcctgggcc tcggccacac ggtcgcgccg tgacagcgcg gtatgcccgc ccgacagcac
     2221 gggctcggct tcggcgtaga gcgtgcgcag cagctggccc ttccagccgt tccacacgcc
     2281 aggccccacc gcgcggatat cggcgacggt gaggatcagg agaagcttca ggcgttcgag
     2341 gctttgcacg acggaagaga aatcgagcac tgtcttgaag tcattgagat cgcgcatctg
     2401 cgccgtttcg ctcatcagca ggtgatggcg gatgagccag gccactgtat cggtctctgc
     2461 tggcgtgagg ccaaaccggg gtccgagct
//
LOCUS       DBNGVR010000136         4866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2013368, whole genome shotgun sequence.
ACCESSION   DBNGVR010000136 DBNGVR010000000
VERSION     DBNGVR010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2013368"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="AC16A0_02935"
     CDS             complement(<1..2359)
                     /locus_tag="AC16A0_02935"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3104316.1"
                     /translation="MSFLQALTFTTPLALGALLLLPVIWWLLRFTPPKPETVRFPPLR
                     LLLDLVNREEQPDKTPWWLMLLRLAIAALVIFGVSHPLYAPGRVDTLRSEPLLLVVDD
                     TWAAAADWDKRVAVLNEILDGAKSADSVVSLATTVPHLRPPSLEPAIADDVRTRVAAV
                     QPQALDADRRGLIDRLRNQFGNAAALRVIWMSDGIDDGAAASFAAGLTTLANGTATVE
                     AILPETSQLPLALAAPGFDGGRISVTALRADDGAARGAKAVARASNGRALSEANLIFA
                     AGQSKVQGVMELPVELRNEIARIEIEGQRNAAAVHLMDDRWRRKSIGLQSGAAAENAQ
                     PLLSPLYYVSRALEPYGDMSEPADPEALKAALDQGLSMLVLADIGVLPAETEDLVRRW
                     VENGGVLVRFAGPRLAGAQDSLIPVRLREGGRSLGSALSWETPQAMQAFPETSPFAGL
                     AIDPAVRITRQVLAEPDAELPDRVWANLEDGTPLVTARREGKGTVVLFHVTANADWSN
                     LPLTGLFVEMLRRIVDVAPAAGGGTAVAADDANAAAGFAPYRSLNGFGDLGDPLPDIR
                     PIPAAQIDSVKASPGTPAGLYRRGGQERAINIMRVGDRLTPIAGLPPAIALRSLAPQP
                     AVPLAPWLFAAAVVLFLLDSLAALTLGGGLNRLRARRAAVSILLVIALLPVSSTAFAQ
                     EKNDDFAMRAALQTRLAYVITGDDSIDTISEEGLKGLNTVLRDRTSVDPGDPVGINIE
                     TDEIVFFPLLYWPVRADATVPSDAALAKIDSFMKNGGTIFFDLRND"
     gene            complement(2356..3237)
                     /locus_tag="AC16A0_02940"
     CDS             complement(2356..3237)
                     /locus_tag="AC16A0_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3104317.1"
                     /translation="MAQAAAALSLTDRAEAAGRVLPPLLVEAERLAATVILGDHGRKR
                     AGPGESFWQYRPYSFGDSTQRIDWHKSARSDRIYIRENEWEAANTLWLWASPAESMTF
                     RSHLSRTSKRDRAELVALACGVLAVRAHERVGAVGAPHAPDHARGTLVKIAQWFLDHQ
                     GPALPAFQRMPRFSSALFLGDFFETPSAVQAAIAPMAEAGVTGHLVQVVDPAEETLPY
                     AGRVEFQEMAGPLKFTSSKTETLRSAYAEKLALHRAALQQLCRSIGWTFTIHRTDEPA
                     ARLLFQIHNLIGGAWKR"
     gene            complement(3242..4240)
                     /locus_tag="AC16A0_02945"
     CDS             complement(3242..4240)
                     /locus_tag="AC16A0_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3104318.1"
                     /translation="MPSINEADRQILADLEAMGERLTKVRAAIGKVIFGQDEVVEQVL
                     IAILGGGHALLVGAPGLAKTKLASTLGRVLGLEEKRVQFTPDLMPADILGSEVLDESE
                     GGRRQFRFVPGPVFCQLMMADEINRASPRTQSALLQAMQEHHVTVAGMRHDLPRPFHV
                     LATQNPIEQEGTYPLPEAQLDRFLLQIDVPYPSLDAERRMLFATTGSAEAEAEKVLST
                     TELMNAQRLTRVIPVGEQVAETILKVVRAARPGPDADDFVNKNVAWGPSPRASQALML
                     GARARALLQGRLSPSPEDVAALLEPVFKHRMALNFTARADGHTIQSTIARLAQLIA"
     gene            4380..>4866
                     /locus_tag="AC16A0_02950"
     CDS             4380..>4866
                     /locus_tag="AC16A0_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HIR3104319.1"
                     /translation="MTTADQRDPANPLRGLADAAKAGCGPAPVHLWNPPFCGDIDMRI
                     AADGTWFYLNSPIGRKPLVQLFASVLRRDPDGKYYLVTPVEKCGIRVDDAPFTAIRMA
                     VQGEGRDRIIRFETHVDDEVTVDAAHPLRFMDEPGTGGLKPYVTVRSNLEALVSRALF
                     YD"
BASE COUNT          800 a         1578 c         1681 g          807 t
ORIGIN      
        1 cgtcattgcg caggtcgaag aagatcgtgc cgccgttctt catgaagctg tcgatcttcg
       61 ccaaagccgc atccgacggc accgtcgcat cggcccggac cggccagtag agcagcggga
      121 agaagacgat ctcgtcggtc tcgatgttga tgccgaccgg gtcgccggga tcgaccgagg
      181 tgcggtcgcg caggacggtg ttcaggccct tcagcccttc ctcgctgatc gtgtcgatgc
      241 tgtcgtcgcc ggtgatcaca taggccagcc gcgtctgcag cgcggcgcgc atggcgaagt
      301 cgtcgttctt ttcctgggcg aatgcagtgc tgctgactgg cagaagcgca atcaccagca
      361 gaatgctcac tgccgcgcgg cgcgcgcgca ggcgattgag tccgccgccc agcgtcaagg
      421 cggccaggct gtccagcaaa aagagaacca cggccgcggc aaagagccag ggcgccagcg
      481 gcaccgcggg ctgcggggcc agcgagcgca aggcaatggc gggcggcagt cccgcgattg
      541 gcgtcaggcg gtcacccacc cgcatgatgt tgatggcgcg ctcctgtccg ccgcggcggt
      601 aaaggccggc cggcgtcccg ggcgaggcct tcacactgtc gatttgggcc gccggaatcg
      661 gacggatgtc gggcagggga tcgccgaggt cgccgaaacc gttgaggctg cggtaaggtg
      721 cgaaccctgc cgcggcattg gcgtcgtcgg ctgcgactgc tgtcccgccg cccgcggccg
      781 gtgccacatc cacgatccgc cgcagcatct ccacgaagag gccggtgagc ggcaggttgg
      841 accagtcggc attggcggtc acgtggaaga ggacgaccgt tcccttgccc tcgcgccggg
      901 cggtgacaag cggcgtgccg tcttcgagat tggcccagac gcggtcgggg agttcggcgt
      961 cgggttccgc aaggacctgg cgggtgatgc gcacggcggg atcgatggca agaccggcaa
     1021 aaggactggt ttcggggaag gcctgcatgg cctggggcgt ctcccagctg agtgcgctgc
     1081 ccagagagcg tccgccctcg cgcagccgca ccgggatcag gctgtcctgt gcgccggcaa
     1141 ggcgcggccc cgcgaagcgc accagcacgc cgccgttctc cacccaccgg cgaaccagat
     1201 cctcggtctc cgccggcaag accccgatat cggccagtac aagcatggaa aggccctgat
     1261 cgagcgccgc cttcagggcc tcgggatcgg ccggctcgct catgtcgcca tagggttcaa
     1321 gggcgcggct cacatagtag agaggcgaaa gcaggggttg ggcgttctcg gctgccgccc
     1381 cggactgcag gccgatgctc ttgcgccgcc agcgatcatc catcagatgc accgccgcgg
     1441 cgttgcgctg gccttcgatc tcgattcggg cgatctcgtt gcgcagctcg accggcaatt
     1501 ccatgacgcc ctgcaccttg ctctggccgg ccgcgaagat cagattggct tcgctcagcg
     1561 cgcggccgtt gctggcccgg gcgaccgcct tggcgccgcg cgctgcgcca tcgtccgcac
     1621 gcagtgcggt gacgctgatg cgcccgccgt cgaagcccgg cgccgcaagt gccagcggca
     1681 gttgcgacgt ctcgggcagg atggcttcga ccgtggccgt gccattggca agtgtcgtaa
     1741 ggccggccgc gaagctggcg gccgcgccat cgtcgatccc gtcgctcatc cagatgacgc
     1801 gcagggccgc ggcattgccg aactgattgc gcagcctgtc gatcagtccg cggcggtcgg
     1861 catcgagcgc ctgcggttga actgccgcga cacgcgtgcg cacgtcatcc gcaatggcag
     1921 gctcgaggct cggtggccgc agatgcggca ccgtcgtggc aagcgacaca acggaatcgg
     1981 cgctcttcgc gccatcgaga atctcgttca gaaccgccac gcgcttgtcc cagtccgccg
     2041 ccgccgccca tgtatcgtcg accaccagca gcagcggttc gctgcgcagc gtgtcgacgc
     2101 gtcccggcgc atagagcgga tgcgacacgc cgaagatcac caaagcggca atggccagcc
     2161 gcagtaacat cagccaccac ggagtcttgt ccggctgttc ctcgcggttg acgaggtcaa
     2221 gcaggaggcg cagtggcgga aagcgcaccg tctccggttt tggcggcgtg aagcgcagca
     2281 gccaccagat caccgggagc agcagcagcg cacccagcgc gagaggcgtg gtgaaggtga
     2341 gggcctgaag gaaactcatc ttttccaggc tccgccgatc agattgtgga tctggaacag
     2401 cagccgcgcg gcgggttcat cggtgcgatg gatggtgaag gtccagccga tgctgcggca
     2461 cagctgctgc agggcggcgc gatgcagcgc cagcttctcc gcataggcgc tgcgcagggt
     2521 ctccgtcttg ctcgatgtga acttcagcgg ccctgccatt tcctggaact cgacgcggcc
     2581 tgcatagggc agggtctcct cggccggatc gaccacctgc acgagatgtc cggttacgcc
     2641 ggcctccgcc atgggagcga tcgcggcctg gaccgcagat ggggtctcga agaaatcacc
     2701 gaggaagagc gccgatgaaa agcgcggcat gcgctggaat gcgggcaatg cggggccctg
     2761 gtgatcgaga aaccactgcg cgatcttcac cagcgtgccg cgcgcatgat cgggggcatg
     2821 gggcgcgccg accgcgccca cgcgttcatg cgcccgcacg gcgaggacgc cgcaggccag
     2881 tgcgacgagt tcggcgcggt cgcgcttgct ggttcgcgag agatgcgagc gaaaagtcat
     2941 cgactcggcc ggcgaagccc agagccacag cgtattcgcc gcctcccatt cgttctcgcg
     3001 gatgtagatg cggtccgaac gcgccgactt gtgccagtcg atgcgctgcg tcgagtcgcc
     3061 gaaactgtag ggacggtact gccagaagct ctcgcccggt ccggcacgct tgcggccgtg
     3121 atcgccaagg atcacggttg cggccagccg ctcggcctcc accagaagcg gcggcagcac
     3181 gcggcccgcg gcttcggcgc ggtcggtgag cgacaatgcg gcggcggctt gggccatggg
     3241 ctcaggcaat gagctgagcg aggcgggcga tggtactctg gatggtgtgg ccgtcggctc
     3301 gggcggtgaa gttcaacgcc atgcgatgct tgaacaccgg ctcgagcagg gcggccacgt
     3361 cctccggcga agggctgagc cggccttgca gcagggcgcg ggcgcgggcg cccagcatca
     3421 gcgcctggct ggcgcgcggc gacggccccc aggccacgtt cttgttgacg aagtcgtcgg
     3481 cgtccggccc tggcctggcg gcgcgcacca ccttcaggat cgtctcggcc acctgttcgc
     3541 ccaccgggat gacacgtgtc agccgctggg cattcatgag ctcggtcgtg ctcagcacct
     3601 tttccgcctc ggcctcggca gaacccgtgg tggcgaacag catgcggcgc tcggcatcga
     3661 gcgaggggta gggtacatcg atctgcagca ggaagcggtc gagctgggct tcgggcaggg
     3721 gataggtccc ttcctgctcg atcgggttct gggtggccag cacatggaag ggccgcggca
     3781 ggtcgtggcg catgccggcc acggtgacat ggtgctcctg catcgcctgc agcaaggcgg
     3841 actgggtgcg cgggctggcg cggttgatct cgtcggccat catcagctgg cagaagaccg
     3901 gtcccggcac gaagcggaac tggcggcgcc cgccctcgct ctcgtccagc acttcggaac
     3961 ccaggatatc ggccggcatc aggtcgggcg tgaactgcac gcgcttttcc tcgagaccca
     4021 gcacccggcc cagcgtgctg gccagtttcg tcttggcaag gccgggcgca cccaccagca
     4081 gggcatggcc gccgccgaga atggcgatca gcacctgttc cactacctcg tcctggccga
     4141 agatgacctt gccgatggcg gcgcgcacct tggtcaggcg ctcgcccatg gcctcgagat
     4201 cggcgagaat ctggcggtct gcttcattga tgctgggcat gacgctcact atagttgtta
     4261 tagttgtttc acgccaatct tggacggcgg aacgggtttg ctggtaatga catcctcgtg
     4321 aatgagaatg gcctaaaaga ggcggttcac gttacgactc atacagggtt tacggtgcga
     4381 tgacgaccgc agatcaaagg gatccggcca atccgctgcg gggcctcgcg gacgccgcga
     4441 aggcggggtg cgggccagcg cccgtgcatc tttggaaccc gcccttctgt ggcgatatcg
     4501 acatgcggat cgccgctgat ggaacctggt tctacctcaa ttcgcctatc ggccgtaagc
     4561 cgttggtgca gctcttcgcc tcggtcctgc gccgcgatcc ggacggcaag tactacctcg
     4621 tcacgcctgt ggaaaaatgc ggcatccgcg tggatgacgc gcccttcacg gcgattcgca
     4681 tggcggtgca gggcgagggc agggaccgca tcatccgctt cgagacccat gtcgatgatg
     4741 aggtgacggt cgatgccgcg catccgctgc gtttcatgga cgaaccgggc accggtgggt
     4801 tgaagcccta tgtgaccgtt cgttccaatc tcgaggcgct tgtctcccgg gcgctgttct
     4861 acgacc
//
LOCUS       DBNGVR010000137         3997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_244472, whole genome shotgun sequence.
ACCESSION   DBNGVR010000137 DBNGVR010000000
VERSION     DBNGVR010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_244472"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="AC16A0_02955"
     CDS             <1..840
                     /locus_tag="AC16A0_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HIR3104320.1"
                     /translation="STDVTPRYVEADPFEGGKQAVAECWRNLTAVGSLPIAITDNLNF
                     GNPERPEIMGQFVKAIEGISAACRALDFPVVSGNVSLYNETNGQGILPTPAIGGIGLL
                     EDLMKMATLAFKSAGDEILLIGGHGTHLGQSIYLREVLGREDGAPPPVDLAVEKRNGD
                     FVRNLINTSQVSAVHDVSDGGLGLAIIEMALAARLGASLLPLTHERLFGEDQARYVVT
                     CAPSAAAAISAAGGKAGIAVDRIGHVTADATLNIAGGPSISLDQLRSAHEGWFPAYMS
                     GEL"
     gene            850..1086
                     /locus_tag="AC16A0_02960"
     CDS             850..1086
                     /locus_tag="AC16A0_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR3104321.1"
                     /translation="MAMSAADIERFIKESLPDAKVEIRDLAGDGDHYAANVISAAFKG
                     KSRVQQHQMVYAALKGRMGGELHALALQTSTPED"
     gene            1090..1410
                     /gene="grxD"
                     /locus_tag="AC16A0_02965"
     CDS             1090..1410
                     /gene="grxD"
                     /locus_tag="AC16A0_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020628855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR3104322.1"
                     /translation="MSDINTWIDNEVKSNDVVLFMKGTPDMPMCGFSGRATQILNHLG
                     VPFKGVNVLDSDDLRQGIKNYTNWPTIPQLYVKGEFVGGSDIMMEMFQSGELKTLMTE
                     KGLG"
     gene            1568..2464
                     /locus_tag="AC16A0_02970"
     CDS             1568..2464
                     /locus_tag="AC16A0_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104323.1"
                     /translation="MDSLKTRAILALLLTMIFWGSAAVFMRTLALALTPENSLALRYV
                     IVSAINVIGLAYLGTWRIPRADWPRFLVAGLAGMAGYNWFVNAGFALVPAGLGTIITM
                     VEPLMIALLAALLLGEKLNRFIFIGVAIATLGAFVLFWEDLTTTQASSVPPLGILYLM
                     ICCLCWAIYTIVAKPLLDRYDSFTVTAITMLIAAPPLIGAANEPLTVLAARLDLRQWA
                     ELIYLVLASGIGGTLLWNYGSKHLSGTTAGTFLYLIPVVAVASGALVLAEPVTIFIVL
                     GGLLMLAGVAAAQFGPSLFSRR"
     gene            2469..>3997
                     /locus_tag="AC16A0_02975"
     CDS             2469..>3997
                     /locus_tag="AC16A0_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104324.1"
                     /translation="MQVQALIALLFTVIVWGVGPVFIRSLSVALGPSDHIVIRYTIVA
                     IIYAIGLLILGGWRIARSDWPRLMLISTVGMIGYNLGSAFGFELVPAGLGSLIIGTQP
                     LLIAATGALIAREKLTPIAMLGLAVGFTGTVLLVWRDLGLSGDSASFIAGSVFIFFCG
                     VAWAIYVVGAKPLIRKYGSYSITALSIIIASLIMIPMLARPSTLGTVMAMTPRNWLDM
                     AYISILSTLIATITWNYGASRLPAAAAGAFIYLVPIIGVAAGAFMLSETITPGMMLGG
                     TMILAGVAIAQFGPLLKLGGKATALAAILFAVTMWGLIPVAMRYLVLELSPQTAMVLR
                     LFPAGLLAIVILTVIGVRRIAPRDWVRIAIAALAGNLGYQILAAYGMETVPASWTGLI
                     FGLEPVFIALFAVLLAGDRLTGWLIAGMTVAMLGTAALMLGSTLLPASDVGLAGLLMV
                     TASTMGWGIYTVVIQPVSRKYGAFEVACLAMGISALPMALFVTPDMPQVLAAMGTTEW
                     LA"
BASE COUNT          636 a         1380 c         1210 g          771 t
ORIGIN      
        1 tcgaccgacg tgacgccgcg ctacgtcgag gccgatccct tcgagggcgg caagcaggcg
       61 gttgccgaat gctggcgcaa cctcacggcg gtggggtcgc tgcccatcgc gatcaccgac
      121 aacctgaact tcggcaatcc ggaacgcccc gagatcatgg gccagttcgt caaggccatc
      181 gagggcatct cggccgcctg ccgcgcactc gacttcccgg tcgtctccgg caatgtctcc
      241 ctctacaacg agaccaatgg ccagggcatc ctgccgacgc ccgccatcgg cggcatcggg
      301 ttgcttgaag atctcatgaa aatggccaca ctggccttca agtccgccgg cgacgagatc
      361 ctgctgatcg gcggccacgg cacgcatctg ggccagtcca tctatctccg cgaggttctg
      421 ggacgcgaag acggagcgcc gccgccggtc gacctggctg tcgagaagcg caatggcgac
      481 ttcgtgcgga acctgatcaa cacctcgcaa gtctctgctg tgcatgacgt ttccgacggt
      541 ggactgggac ttgccatcat cgagatggcg cttgccgcaa ggcttggcgc cagcctgctg
      601 ccattgaccc acgaacggct cttcggcgag gaccaggcgc gctatgtcgt gacctgcgca
      661 ccttccgcgg cagcggcgat ctcggcggcg ggaggcaagg ccggcatcgc cgtggaccgg
      721 atcggccatg tgacggccga cgccacgttg aacatcgccg gagggccctc tatatctctc
      781 gatcaactcc gctctgccca tgaaggctgg ttcccggcct acatgtcagg ggaactgtag
      841 gaacacgata tggcaatgtc cgccgccgac atcgagcgct tcatcaagga gtcgctgccg
      901 gatgcgaagg tcgagatccg cgacctcgcg ggcgatggcg accactatgc ggccaatgtg
      961 atctccgccg ccttcaaggg caaatcgcgg gtgcagcagc accagatggt ctatgccgcc
     1021 ctgaagggcc gcatgggcgg cgaactccat gcccttgcac tccagaccag cacaccagag
     1081 gactgaccga tgagcgacat caacacctgg atcgacaatg aagtgaagag caacgacgtc
     1141 gtgctgttca tgaagggcac gcccgacatg cccatgtgcg gcttttcggg ccgcgccacc
     1201 caaatcctca atcaccttgg cgtgcccttc aagggcgtca atgtcctcga ctcggatgac
     1261 ctgcgacagg gcatcaagaa ctacacgaac tggcccacca ttccgcagct ctacgtgaag
     1321 ggcgagttcg tcggcggttc ggacatcatg atggagatgt tccagtccgg cgaactcaag
     1381 accctgatga ccgagaaggg cctgggctaa acgcccatca tgagagattt cggaaggccg
     1441 ggcctcgtgc ccggcctttt ccgtttggca gcagagcttg cccttggccg catgcctcct
     1501 ccgcaggccc gccaattgac cggagcgcgc cgttcgggca cccatggtct tctcatccgg
     1561 attcttcatg gacagcctca agacccgcgc cattcttgcc ctcctgctga ccatgatctt
     1621 ctggggatcg gcagcggtct tcatgcgcac gctggcgctg gcgctgacgc ccgagaattc
     1681 gctggcgctg cgctatgtca tcgtcagtgc gatcaatgtc atcggcttgg cctacctcgg
     1741 cacctggcgc atcccgcgcg ccgactggcc gcgcttcctg gtcgcgggcc tggccggcat
     1801 ggccggctac aactggttcg tgaatgccgg cttcgcgctg gtcccggccg ggctcggcac
     1861 catcatcacc atggtcgaac ccctgatgat cgccctgctc gccgccctgc tgctgggcga
     1921 gaagctcaac cgcttcatct tcatcggcgt ggccatcgcc acgctcggcg ccttcgtcct
     1981 gttctgggaa gacctcacca ccacgcaggc ctcatcggtc ccgccgctcg gcatcctcta
     2041 cctgatgatc tgctgcctgt gctgggccat ctacaccatc gtcgccaagc ccttgcttga
     2101 ccgctacgac agcttcacgg tgacggccat caccatgctg atcgcggccc cgccactgat
     2161 cggcgccgcc aacgagccgc tgacggtgct cgctgcgcgc ctcgatctgc ggcaatgggc
     2221 tgaactcatc tatctcgtcc tcgccagcgg cattggcgga acgctcttgt ggaactacgg
     2281 cagcaagcat ctctcgggca cgacggccgg caccttcctc tatctcatcc cggtcgttgc
     2341 cgtggccagc ggcgcgctcg tcctggcgga gccggtgacc atcttcatcg tgctgggcgg
     2401 cctgctcatg ctggccgggg tggcggccgc gcagttcgga ccaagcctgt tctcgcggcg
     2461 ctgagccgat gcaggtccag gccctcatcg ccttgctctt caccgtcatc gtctggggcg
     2521 tggggccggt tttcatccgc agtctctcgg tggccctggg gccatccgac cacatcgtca
     2581 tccgctacac catcgtggcg atcatctatg cgatcgggct gctgatcctc ggaggctggc
     2641 gcatcgcgcg ctcggactgg ccgcgactga tgctgatctc caccgtcggg atgatcggct
     2701 acaatctggg ctctgcattc ggcttcgaac ttgtcccggc cggcctgggc agcctgatca
     2761 tcgggacgca gcccctgctg atcgccgcca cgggcgcgct catcgcccgc gaaaagctaa
     2821 cacccatcgc catgcttggc ctggcggtcg gcttcaccgg aaccgtgctg ctggtctggc
     2881 gcgaccttgg gctgtcgggc gactccgcca gcttcatcgc cggcagcgtc ttcatctttt
     2941 tctgcggcgt cgcctgggcc atctatgtcg tcggggccaa gccgctgatc cggaagtacg
     3001 gttcctacag catcaccgca ctctccatca tcattgcctc cctgatcatg atccccatgc
     3061 tggcacggcc ttcgaccctc ggcacggtca tggccatgac gccacgcaac tggctggaca
     3121 tggcctatat ctcgatcctg tcgacgctga tcgccaccat cacctggaac tacggcgcat
     3181 cgcgcctgcc cgcagccgct gcgggcgcct tcatctatct ggttccgatc atcggcgttg
     3241 ccgccggcgc cttcatgctg agcgagacca tcacgccggg catgatgctg ggcggaacga
     3301 tgatcctggc cggcgttgcc atcgcgcaat tcggtcccct gctgaaactc ggcggcaaag
     3361 cgacagcact agcggccatc ctcttcgcgg tgacaatgtg gggcctgatc ccggtggcca
     3421 tgcgctacct cgtgctggag ctgtcgcccc agacggcgat ggtgctgcgc ctctttccgg
     3481 cgggcctcct cgccatcgtc attctcaccg tcatcggggt aaggcgcatt gcgccacgcg
     3541 actgggtccg catcgcaatc gccgcccttg ccggaaacct cggctaccag atcctggccg
     3601 cctatggcat ggagaccgtt ccagcaagct ggaccgggct gatcttcggc ctggaaccgg
     3661 tcttcatcgc gctgtttgcc gtgctgctgg cgggcgaccg gctgaccggc tggctcatcg
     3721 ccggcatgac ggtcgcgatg ctggggacgg ccgcgctgat gttgggcagc accctgctgc
     3781 ccgcctccga tgtggggctt gcgggcctgc tcatggtgac ggcaagcacc atgggctggg
     3841 gcatctacac ggtggtgatt cagccggtct cgcggaagta cggagccttc gaagtggcct
     3901 gcctcgccat gggcatctcg gcgctcccca tggcgctctt cgtgacgccc gacatgccgc
     3961 aggttctggc ggccatgggc acgaccgagt ggctggc
//
LOCUS       DBNGVR010000138         1567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4173212, whole genome shotgun sequence.
ACCESSION   DBNGVR010000138 DBNGVR010000000
VERSION     DBNGVR010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4173212"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC16A0_02980"
     CDS             complement(<1..484)
                     /locus_tag="AC16A0_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3104325.1"
                     /translation="MTRPIRHFLDLTQFGAEEIRGILQASVDMKRARKRGERQAVRPL
                     EGKTLAMIFDKPSTRTRVSFDVAMRELGGETIMLTGAEMQLGRGETIGDTARVLSRYV
                     DAIVIRMLDQNAVRELAEGATVPVINGLTKASHPCQIMADVMTFEEHRGSIKGRTIAW
                     V"
     gene            complement(481..>1567)
                     /locus_tag="AC16A0_02985"
     CDS             complement(481..>1567)
                     /locus_tag="AC16A0_02985"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HIR3104326.1"
                     /translation="GIAVNALGHAHPHLVKALTDQAQRVWHVSNLYHIPAGERLAQRL
                     VDSTFADHVFFTNSGAEAIECAIKMARKYHSANGQPERFRVITFEGAFHGRTLATIAA
                     GGQKKYLEGFGPKVDGFDQVPFGDHKALEAAIGPETAAILIEPIQGEGGVRSVPPQCL
                     RGLRELCDKQGLLLIFDEVQTGVGRTGRLFAYQRAGVEPDIMAIAKGIGGGFPMGACL
                     STREAGKGMTAGVHGTTFGGNPLAMAVGNAVLDVILEPGFLEKVEHTSLLLKQRLAEL
                     KDRHPGIIEEIRGEGLLIGIKCRVPNTELATAARGQKLLTVPAGDNVLRLLPPLNIDE
                     TIVGEAFARLDRACAQIEASQVKGAAE"
BASE COUNT          261 a          520 c          482 g          304 t
ORIGIN      
        1 ctacccaggc gattgtgcgg cccttgatcg atccacgatg ctcctcgaac gtcatgacat
       61 cggccatgat ctggcacgga tgcgacgcct tggtgagacc gttgatgacg ggcaccgttg
      121 cgccttcggc cagttcacgc accgcattct ggtccagcat cctgatcacg atggcgtcga
      181 cgtagcgaga caggactcgc gccgtgtctc cgatcgtctc cccgcgccca agctgcatct
      241 ccgcgccggt gagcatgatc gtctcgccgc ccaactcgcg catcgccacg tcgaacgaga
      301 cgcgcgtacg ggtggagggc ttgtcgaaga tcatcgccag cgtcttgcct tcgagaggcc
      361 gcactgcctg gcgctcgccg cgcttgcgcg cgcgcttcat gtcgaccgag gcctgcagaa
      421 tgcctctgat ttcctcggcg ccgaactgag tgagatccag gaagtgcctg attggtcttg
      481 tcattccgcc gctcccttca cctgcgacgc ctcgatctgg gcgcaggcgc gatcgagccg
      541 cgcaaaggct tctccgacga tggtttcatc gatgttcaag gggggaagca gcctgagcac
      601 attgtcgccg gccggcacgg tcaacagctt ctggccgcgg gcggccgtgg caagttccgt
      661 attcgggacg cggcatttga tgccgatcag caggccttcg cccctgatct cctcgatgat
      721 cccggggtgg cggtctttca actcggcaag ccgctgcttg agcaacaggc tggtatgctc
      781 gaccttttcg aggaaacctg gctccaggat gacatcgagc actgcattgc cgaccgccat
      841 tgcaagcgga ttgccgccga acgtcgtgcc gtgaacgccc gctgtcatgc ccttgcccgc
      901 ttcgcgcgtc gagaggcagg cacccatcgg gaagccgccg ccaatgccct tggcgatagc
      961 catgatgtcg ggctcgacgc cggcgcgctg gtaggcaaac agcctgccgg tccggccgac
     1021 gccggtctgg acttcgtcga aaatcagaag gaggccctgc ttgtcgcaga gttcccgcaa
     1081 gccgcgcagg cattgcggcg gcacgctccg cacgccgcct tctccctgga tcggttcgat
     1141 caggattgcg gccgtctccg gtccaattgc agcttcgagc gccttgtggt ccccgaacgg
     1201 cacctggtcg aagccgtcga ccttcggacc gaagccctcg aggtatttct tctgcccgcc
     1261 tgctgcgatc gtcgccagcg tgcgtccgtg gaacgcaccc tcgaaggtga tcacgcgaaa
     1321 ccgctcgggc tgaccattgg ccgaatggta cttgcgagcc atcttgatcg cgcattcaat
     1381 cgcctcggcg cctgaattcg tgaaaaagac atggtccgcg aaggtgctat cgacgagacg
     1441 ctgcgccagc cgctcgcctg ccgggatgtg ataaaggttt gagacgtgcc agacgcgctg
     1501 cgcctggtca gtcagcgcct tcaccagatg cggatgcgcg tggccgagcg cattcacggc
     1561 gatgccg
//
LOCUS       DBNGVR010000139         6952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_245090, whole genome shotgun sequence.
ACCESSION   DBNGVR010000139 DBNGVR010000000
VERSION     DBNGVR010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_245090"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1868
                     /gene="ilvD"
                     /locus_tag="AC16A0_02990"
     CDS             144..1868
                     /gene="ilvD"
                     /locus_tag="AC16A0_02990"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3104327.1"
                     /translation="MDKPVIDKTKLPSRHVSVGPERAPHRSYYYAMGLTPQQINQPFV
                     GVASCWNEAAPCNIALMRQAQAVKKGVAAAAGTPREFCTITVTDGIAMGHQGMKASLV
                     SREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSVFIYGGSILPGTF
                     KGQPVTIQDVFEAVGKHSVGAMSDDDLLELEQKACPSAGSCGAQFTANTMATVAEAIG
                     LALPYSCGAPAPYEIRDTFCFTAGEKVMELLARNIRPRDIVTRKALENAATVVAASGG
                     STNAALHLPAIAHEAGISFDLFDVAEIFRRTPYIADLKPGGRYVAKDLFEVGGIPLLM
                     KTLLDHGYLHGDCVTVTGRTMAENMASVKWNPDQDVIRPADRPFTRTGGVVGLKGNLA
                     PEGAIVKVAGMKTLRFSGPARCFDTEEACFEAVSKRKYKEGEVLVIRYEGPRGGPGMR
                     EMLATTAAIYGQGMGDKVALITDGRFSGATRGFCIGHVGPEAQLGGPIGLLQDGDVIT
                     IDAEAGTLDCNVSEAEFAQRRASWQPRAHGYNSGTLWKYAQQVGPAFSGAVTHPGGAA
                     EGMAYADI"
     gene            complement(1932..2222)
                     /locus_tag="AC16A0_02995"
     CDS             complement(1932..2222)
                     /locus_tag="AC16A0_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HIR3104328.1"
                     /translation="MIISKMEFLYRSHLDQETLQVWIEEEWLVPQVSEPDVTFTEADV
                     ARAQLILDLKKDLGVNDEGIGVILNLIDQMHSLRRALAGKSGARGSFPGEDS"
     gene            complement(2225..3127)
                     /locus_tag="AC16A0_03000"
     CDS             complement(2225..3127)
                     /locus_tag="AC16A0_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003571986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR3104329.1"
                     /translation="MKDPYEILGVARGASADELQKAYRKLAKKLHPDLNPGDKTAEER
                     FKQVAGAYDLLSDPEKRRRFDAGEIDALGAERPRERYYRDYASAAASGSPYENPSGFA
                     DFAQNDDFLAELLRRQARQARQAPGADVHYRLAVDFLDAINGANRRLTLPDGGSIDVS
                     IPPGVREGQVLRLRGKGAPSRGEGPPGDALVEIEVKPHRHFTLQGDDILLDLPVTIQE
                     AALGGSVRVPTPSGSVMMTIPKGSNSGTTLRLKGKGAARRGGGNGDQLVKLKVMLPSE
                     PNAELNAFLSKWAPGPNDNPRRDM"
     gene            complement(3214..4296)
                     /locus_tag="AC16A0_03005"
     CDS             complement(3214..4296)
                     /locus_tag="AC16A0_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR3104330.1"
                     /translation="MDKDWIDIGEAAELASQSVRRCRAGNTEIALTCVNGLFGAVHNA
                     CNHVGGPLGEGTLQGDYLVCPWHQWRFNRITGLGEPGFENDKVPSYPVKVEGGRVLVN
                     TGAGTSRNRLPHDPITLARPVVRAEGAIRVAGISTTAMDGRAPRYSGSDDLLGSALAG
                     AKAEGCETQMIRLNDLKFHACEGYYSKAARACTWPCSITQMRSDDELTAVYEALVHWA
                     DVILIATPIRWGQASSLYFKMAERLNCIQNQITTHNRVLIRNKVAGFIIVGGQDNIQG
                     VAGQMLGFFAELGFQFPPFPYIAHSRGWSAEDMENNVTMVRNSRELHEGAAELARRSV
                     DLSRRLLETAPIERLSRGGRKAHAER"
     gene            4539..5486
                     /locus_tag="AC16A0_03010"
     CDS             4539..5486
                     /locus_tag="AC16A0_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR3104331.1"
                     /translation="MQSRDWGALMGEAHNLSSLRGAAANARLRLGVRVEPPIPLDVED
                     VREREVRLAALAAEEVIPRLLAIHNQIIVPVDHDRLHAGSIEIEELSRLVLRPDGPEA
                     LDYIQLLRDRGLSLDQLHEELLEPTARHLGELWEADRIDFVDVAVGVSKLQRMVHYFA
                     RLDAIPPYDDRLKALITTTPGDSHSFGNAIVQKFLRASGWHVCACSAPGPDELASLVA
                     DEWFGVVGFSVSADQHLDALASAIRRVREVSLNRTIGIMVGGPCFESHDERVSMVGAD
                     GTARNGPAAAILAKKLLVRSLAFTDLPSSPLHPPFDTPT"
     gene            complement(5515..6069)
                     /locus_tag="AC16A0_03015"
     CDS             complement(5515..6069)
                     /locus_tag="AC16A0_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019501.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HIR3104332.1"
                     /translation="MSIRFLAVPMLMAMLGFGAGPSQAFPAPSSEQVSQATTGVTLAA
                     NRSSYVSGRKSYGGRSAYRKRWIRRKGHAGNWHGHHGHWNNHKKHYPRYWRARPHYYY
                     PGYFNWGYCGWRYGALPYDCFGNDDYYGDDDYDVPAKRVRTSNSMHIKWCKARYKTYN
                     VKTDTFIGKGKKKYRCNSPYDGRR"
     gene            complement(6326..>6952)
                     /locus_tag="AC16A0_03020"
     CDS             complement(6326..>6952)
                     /locus_tag="AC16A0_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3104333.1"
                     /translation="WPILSARTFPLPKYPVYGHLNDCSVLLESVIGHPGGAFRFYVTH
                     LNYLSERQRLIQTRMLIDIIADAPRQGGPVTGPGVPASEFGADWIALEKDEVPSMPEP
                     AILLGDFNMMPSSPEYALLTGPAGPDYGRIPEYGLFVDALTAAGQQESEGITYPANDI
                     EQAKRIDHILLTSDLAGRVRRAWIDEAADASDHQPVYAELDWGEASAG"
BASE COUNT         1275 a         2149 c         2207 g         1321 t
ORIGIN      
        1 cccatctcga actggaccag attccaggtc tggctctaaa ttcggaacga gaccacagca
       61 ctgtcatcgc cctcattctc cagagagtgg caaaacgacc gggacacccc tacattcgcc
      121 aagcacacgg aattggagtc agcatggaca agccagtcat cgacaagacc aagctgccca
      181 gccgccatgt cagcgtgggg ccggagcgcg cgccgcaccg gtcctattac tacgcgatgg
      241 gcttgacgcc gcagcagatc aaccagccct tcgtgggtgt cgcctcctgc tggaacgaag
      301 ccgcaccctg caacatcgcc ctgatgcggc aggcccaggc ggtgaagaaa ggcgtggccg
      361 cggctgccgg cacaccgcgc gaattctgta cgatcacggt gacggatggc atcgccatgg
      421 gccaccaggg catgaaggcc tcgctggtct cgcgcgaggt catcgccgac tccgtcgaac
      481 tcacgatgcg cgggcattgc tacgatgcgc tggtcgggct tgccggttgc gacaagtccc
      541 tgccggggat gatgatggcc atggtgcggc tcaacgtccc ctctgtcttc atctacggcg
      601 ggtcgattct gcccggtacc ttcaagggcc agccggtgac cattcaggac gtcttcgagg
      661 ccgttggcaa gcattcggtg ggcgccatgt ccgatgacga cctgcttgaa ctggagcaga
      721 aggcctgccc ttcggcgggt tcctgcggcg cacagttcac ggccaatacc atggccactg
      781 tggcggaggc gatcggcctc gcgctgccct attcctgcgg cgcgccggcg ccttacgaga
      841 tccgcgacac cttctgcttc actgcgggcg agaaagtgat ggagctgctc gcgcgcaaca
      901 tcaggccgcg cgacatcgtc acccgcaagg ccctggagaa cgcagcaacc gtcgtcgccg
      961 cctcgggcgg atcgaccaat gcggcgctgc atcttcccgc catcgcgcac gaggcgggca
     1021 tctcgttcga tctcttcgac gtggcggaga tcttcaggcg gacaccctac attgccgacc
     1081 tgaagcccgg tggccgctac gtggccaagg atctctttga agtcggcggc atcccgctgc
     1141 tcatgaagac cttgctcgat cacggctacc tgcatggcga ctgcgtgacg gtgacaggaa
     1201 gaaccatggc cgagaacatg gcgagtgtga aatggaatcc cgatcaggac gtcatccgtc
     1261 cggcggatcg gcccttcaca aggacgggcg gcgtcgtggg gctcaagggc aatctcgcgc
     1321 ccgaaggcgc catcgtgaag gtcgctggca tgaagactct tcgcttctct ggtcctgcgc
     1381 gatgcttcga tacggaggag gcatgtttcg aggcggtatc gaaacgcaag tacaaggaag
     1441 gcgaagtgct ggtgatccgc tatgagggac cgcgcggcgg ccccggcatg cgcgagatgc
     1501 tggcgaccac cgctgccatc tacgggcagg gcatgggcga caaggtggca ctgatcaccg
     1561 acggccgctt ctcgggcgcc acgcgcggat tctgcatcgg ccatgtcgga ccggaggcgc
     1621 agctgggtgg gccgatcggc ctgcttcagg acggcgacgt cattaccatc gacgcggaag
     1681 ccggcacgct ggattgcaat gtcagcgagg ccgagttcgc gcagcgccgt gccagctggc
     1741 agccgcgtgc ccatggttac aactcgggca cgctctggaa atatgcccaa caggtaggcc
     1801 ccgccttctc tggtgcagtg acgcatcccg gcggcgcggc cgaaggcatg gcatacgcgg
     1861 acatttgaat gtgatcagcc ctcttcgagg accggtctga gagcgagtag ctgggcggcc
     1921 tcgccgccgc ctcaggagtc ctcgccggga aagctgcccc tggcgccaga ctttccagcg
     1981 agtgccctgc gcaggctgtg catctggtcg atcaggttca agatgacgcc gataccctcg
     2041 tcgttgacgc cgaggtcctt cttgagatcg aggatcaatt gggcacgggc gacatccgcc
     2101 tcggtgaagg tgacgtcagg ctcgctgacc tgtggcacaa gccattcctc ctcgatccag
     2161 acctgcaagg tttcctgatc gaggtgggag cggtaaagaa actccatctt gctgatgatc
     2221 atggctacat gtcccggcgc ggattgtcgt tcgggcctgg agcccatttc gagaggaacg
     2281 cgttgagttc ggcattgggt tcgcttggca gcatgacctt gagcttcacc agctggtcac
     2341 cgttgccgcc gccgcggcgc gctgcgccct tgcccttgag ccgcaaagtg gttccgctgt
     2401 tcgatccctt gggaattgtc atcatgaccg agccgcttgg cgtgggcacg cggacgctgc
     2461 cgcccagtgc ggcttcctgg atggtgaccg gaaggtcgag gagaatatcg tcgccctgca
     2521 aggtaaaatg gcggtgtggc ttgacctcga tctcgaccag agcgtcgccg ggcggtcctt
     2581 cgcctcgcga gggggcgccc ttgccgcgca ggcggaggac ctgtccttcg cgcacgcccg
     2641 gcggaatact gacgtcgatc gatccgccat cggggagggt cagccgacgg ttggcgccat
     2701 tgatggcgtc gaggaagtcc accgcaagcc gatagtgaac atctgcacct ggcgcctggc
     2761 gggcctgacg ggcctgacgc ctgagcagct cggccaggaa atcgtcgttc tgtgcgaagt
     2821 cggcaaatcc tgacgggttc tcgtatgggc ttccactggc cgccgccgac gcatagtcgc
     2881 gatagtaacg ttcgcgcggg cgctctgcac ccaaggcatc gatctcgccg gcatcgaagc
     2941 ggcggcgctt ctcgggatca gacaacaggt cataggctcc agccacctgc ttgaagcgct
     3001 cctccgctgt cttgtcgccg ggattgagat cggggtgcag cttcttcgcc agcttgcggt
     3061 aggctttctg gagttcatcg gccgaagcgc cgcgcgccac cccaagaatc tcgtaagggt
     3121 ccttcactgt cgttccttct ggcaggccgg tgggtttgct ctaaagacta ctttgaaagg
     3181 cacgaaagta aagtacggcg ggcatgcatg gcactagcgc tcggcatgcg ccttgcggcc
     3241 accacggctc agcctttcga tcggtgccgt ttcgagcagc cggcgcgaca ggtcgacgga
     3301 tcgtcgcgcc agttccgccg cgccctcgtg cagttccctg gaatttctga ccatcgtgac
     3361 gttgttctcc atgtcctcgg ccgaccagcc gcggctgtgt gcgatatagg ggaagggcgg
     3421 gaactggaaa cccaactcgg cgaaaaatcc gagcatctgg ccggcgacgc cctggatatt
     3481 gtcctggccg ccgacgatga tgaagcccgc aaccttgttg cggatcagca cgcggttgtg
     3541 ggtggtgatc tgattctgga tgcagttcag ccgctcggcc atcttgaagt agagcgaact
     3601 ggcctgtccc cagcggatgg gcgtggcaat caggatcaca tcggcccagt gcacaagggc
     3661 ctcgtagacg gcggtcagtt cgtcgtccga gcgcatctgc gtgatcgagc agggccaggt
     3721 gcaggcccgc gcggctttcg agtagtagcc ttcgcaggca tgaaacttga ggtcgttcag
     3781 cctgatcatc tgggtctcgc atccttcggc cttggccccc gcaagagcgg agccgagcag
     3841 gtcgtccgat ccggagtagc gtggcgcgcg gccgtccatg gcggtggtcg aaatgcccgc
     3901 cacgcgaatt gcgccctcgg cacgaaccac aggccgggcc agggtaatgg gatcgtgcgg
     3961 aagccggttg cgtgaggtgc cggcgcccgt gttgaccagc acgcggccgc cttccacctt
     4021 caccggatag ctgggaacct tgtcgttctc gaagccgggc tcgccaagcc cggtgatgcg
     4081 gttaaagcgc cactgatgcc aggggcacac cagatagtcg ccctgcagcg tgccttcgcc
     4141 gagcgggcca ccgacatgat tgcaggcatt gtgaacggcg ccgaacaggc cgttgacgca
     4201 ggtgagcgcg atctccgtgt tgccggcgcg gcagcgacga acagattgcg atgcgagctc
     4261 cgccgcttcg ccaatgtcaa tccaatcctt gtccatgggg tcttgctctc ctgcatgaga
     4321 atcgccagag ggaagtattg gcggaaacgg caacagatgg gaaccgcggc ttcgccgcat
     4381 cgtcttttcg atgacaggtc aagcacatgt tgcaaagcag cgagaaggct tcgcgggaat
     4441 tgttggaagg catccgcggg atgcttgcca agtgcgcccg gaatgcatac ggtattatca
     4501 aagctgcacc caagacagcg aaggttgctg cgcaagtcat gcaatcgaga gactgggggg
     4561 cgctcatggg cgaggcacac aatctgtctt cactacgggg ggccgcagca aacgcgcggc
     4621 tgcggcttgg tgtcagagtt gaacctccca tcccgctgga tgtcgaagac gtccgcgaac
     4681 gggaagtccg gctggcggca cttgcggctg aagaggtcat ccctcgcctg ctcgccattc
     4741 acaatcagat catcgtgccg gtggatcatg atcgccttca cgcgggttcg atcgaaatcg
     4801 aagagctctc acggctggtg ctaaggcccg atgggccgga ggcgctcgac tacatacagc
     4861 ttctccggga tcgcgggctg tcgctcgatc agctccatga ggaacttctc gagccgacgg
     4921 cccggcatct tggagaactg tgggaggccg accgcatcga cttcgtcgat gtggccgtgg
     4981 gtgtctcgaa gctgcaacgc atggtgcact actttgcgcg gcttgatgcc attccaccat
     5041 atgatgaccg gctcaaggcc ctgatcacca ccacacccgg cgatagccac agcttcggca
     5101 atgcgatcgt ccagaaattc ctgcgggcca gcgggtggca cgtctgcgcc tgttctgcgc
     5161 ccggccctga tgaactggcc agtctcgtcg cggacgagtg gttcggtgtc gtggggttct
     5221 cggtcagtgc cgatcagcac ctcgatgcac tggcctccgc catccggcgc gtgcgcgagg
     5281 tttccctcaa tcgcaccatc ggcatcatgg tcggcggacc atgcttcgag agtcatgacg
     5341 agcgcgtttc gatggtcggg gccgatggca ccgcacggaa cgggcctgcg gcggcgatcc
     5401 tggcaaaaaa gctgctcgtg cggagcctgg catttaccga ccttccgtct tcacccctgc
     5461 atccgccgtt cgatacccca acctgaacaa tgccgcaggc ggcgcagctg cgggtcagcg
     5521 ccgcccgtca taggggctgt tgcagcggta cttcttcttg ccttttccaa tgaaagtgtc
     5581 ggtcttgaca ttgtaggtct tgtaacgcgc cttgcaccac ttgatgtgca tgctgttcga
     5641 cgtccggact ctctttgccg gcacatcata gtcgtcgtcg ccatagtagt cgtcgttgcc
     5701 gaaacagtca tagggcagcg caccgtatct ccagccgcag tatccccagt tgaagtagcc
     5761 gggatagtag taatggggcc gcgcgcgcca atacctcgga taatgcttct tgtgattgtt
     5821 ccaatggcca tgatggccgt gccaattgcc ggcatggccc ttgcgacgga tccagcgctt
     5881 tcggtaggcg gacctaccgc cgtagctctt gcggccgctg acgtaactcg accggtttgc
     5941 cgcaagcgtc acgcccgtcg tggcctgact tacctgctcc gatgatggtg caggaaaggc
     6001 ctgggatggc cctgcaccaa agcccagcat ggccataagc atgggaaccg caagaaacct
     6061 gatggacata ctgtagctcc gaattccgcg cgcaccagct ggaccgtatt cactttgctc
     6121 tcatgggcgc gcattttgtc atagcagagt ccctcatgtg agcgatgaca acgccgcatt
     6181 tcggaaaccc tgaattcaca ggcaagttta gccgggcgaa tggaacccgg cgttgccatg
     6241 gatcaaatcg cagatacccg cggtatggtg ggagggcgtg ccgcgacaac ccctgcgcga
     6301 aattcctcac tcctggccct gcagatcaac ccgcagatgc ctcgccccaa tcgagttcgg
     6361 catagaccgg ctgatggtcg gaggcatcgg ccgcctcatc gatccaggcc cggcgcaccc
     6421 gtccggccag atccgaagtg agcaggatgt gatcgatccg cttggcctgc tcaatgtcgt
     6481 tggccggata ggtgattccc tcgctctcct gttggccggc ggcggtgagc gcatcgacga
     6541 agagaccata ctccgggatc cgcccgtaat ccggccccgc aggtccggtg agcagggcat
     6601 actccggcga gctgggcatc atgttgaagt cgcccagcag gatggcgggt tccggcatgg
     6661 acggcacctc gtccttttca agcgcaatcc agtcggcgcc aaactccgag gctggaacgc
     6721 ccggaccggt caccgggccg ccctgccgcg gcgcatcggc aatgatgtcg atgagcatcc
     6781 gggtctgaat caggcgctgg cgttcggaca gatagttgag atgcgtcacg tagaagcgga
     6841 aagccccgcc cggatgaccg atgaccgact cgagtagaac gctgcagtcg ttcaagtgac
     6901 cgtagacggg atacttgggc agtggaaatg tcctggcgga caggatgggc ca
//
LOCUS       DBNGVR010000140         3127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4173470, whole genome shotgun sequence.
ACCESSION   DBNGVR010000140 DBNGVR010000000
VERSION     DBNGVR010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4173470"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1459
                     /gene="gabT"
                     /locus_tag="AC16A0_03025"
     CDS             158..1459
                     /gene="gabT"
                     /locus_tag="AC16A0_03025"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - GABA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HIR3104334.1"
                     /translation="MNAPVAAPGMNKQWVERRAKAVSRGISPGAPVIAARAENSEIWD
                     VEGNRYIDFGGGIAVVNTGHRHPLVMKRVYEQLEAFTHTCAMVMPYAPFIEVCEKLNA
                     VAPISGEKKSALVTTGAEAVENAIKFARAYTGRSDVIAFNGGFHGRTLLGMTLTGKVL
                     PYKAKFGPMAPGVWHVPFPVAHKGVTVEDSMHAIEWLFKADVEPSRVAAIIIEPVQGE
                     GGFYVAPKELMVRLRKLCDDHGILLIADEIQSGFGRTGKFFAMEHYGVEADLMTAAKS
                     LAGGFPLAAVVGRAKIMDAPDPGGIGGTYAGNPVACAAALGVFDAFEQEGLLEKAEKQ
                     GKTIMARMQAIKSQKKGMPIGDIRGLGAMCAFEVVTKHGGNDPDAAGAKALAAKCLER
                     GLLILTCGVYSDTVRLLTPLTASDMVLNEGLDILESAILGN"
     gene            complement(1466..1792)
                     /locus_tag="AC16A0_03030"
     CDS             complement(1466..1792)
                     /locus_tag="AC16A0_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HIR3104335.1"
                     /translation="MAPLVVRQYDVCRNGDRSSRSRIPFLIVLQSDLLSPLETVVVAP
                     VVAEREAQTIKRLNPRLMLSGKAYRVVMQELAGIPRQRLGQAVANVRDRHAEFVGAAD
                     LLFTGV"
     gene            complement(1777..1971)
                     /locus_tag="AC16A0_03035"
     CDS             complement(1777..1971)
                     /locus_tag="AC16A0_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HIR3104336.1"
                     /translation="MNLSVNAQLLEAARAQGINLSAVLQHALAQQAAQRWLDENRTAI
                     EAYNEEVRANGVWSDGWRRW"
     gene            2179..3087
                     /locus_tag="AC16A0_03040"
                     /pseudo
     CDS             2179..3087
                     /locus_tag="AC16A0_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109670.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT          586 a         1037 c          959 g          545 t
ORIGIN      
        1 aaattgaccc cgctctcagg ccatcgacga tctgcgttgc ggtcatgcgg ataagatcgg
       61 acatgcggaa ttccttgttt cagcgccgga ttgaatccgt ttgccgccac cccgtcaaca
      121 ggcgtaaaag gccgccatca ttcgcaagga gaacatcatg aacgcaccgg tcgcagcccc
      181 aggcatgaac aagcagtggg tggaacggcg cgccaaggcc gtgtcccgcg gcatcagccc
      241 gggcgcccct gtcatcgcgg cgcgcgcaga aaactccgaa atttgggacg tcgagggcaa
      301 ccgctacatc gattttggcg gcggcatcgc cgtggtcaat accggccacc gccatccgct
      361 ggtgatgaag cgcgtctatg agcagttgga agccttcacc cacacttgcg ccatggtcat
      421 gccctacgcg cccttcatcg aagtctgcga gaagctgaac gccgtggccc ccatctcggg
      481 cgagaagaag tcggcgctgg tcaccaccgg tgccgaggcg gtggagaacg ccatcaagtt
      541 cgcccgcgcc tataccggcc gctccgatgt catcgccttc aacggcggat tccacggccg
      601 caccctgctg ggcatgacgc tcaccggcaa ggtgcttccc tacaaggcca agttcggccc
      661 catggcgccg ggcgtctggc atgttccctt cccggtcgcg cacaagggcg tgacggtgga
      721 ggactcgatg catgccatcg agtggctgtt caaggccgat gtggagccct cgcgcgtcgc
      781 cgccatcatc atcgagccgg tgcagggcga aggcggcttc tatgtcgcgc cgaaggaact
      841 catggtgcgc ctgcgcaagc tctgcgacga ccacggcatc ctgctgatcg ccgacgagat
      901 ccagtcgggc ttcggccgca ccggcaagtt cttcgccatg gaacattatg gcgtggaggc
      961 cgatctcatg acggcggcca agtcgctggc tggcggattc ccgctggcgg ccgttgtggg
     1021 ccgggccaag atcatggatg cgccggatcc gggtggcatc ggcggcacct atgccggcaa
     1081 tccggtggcc tgcgccgcgg ccctcggcgt cttcgacgcc ttcgagcagg aggggctgct
     1141 ggagaaggcc gagaaacagg gcaagaccat catggcccgc atgcaggcga tcaagtccca
     1201 gaagaagggt atgccgatcg gcgacatccg cgggcttggc gccatgtgcg ccttcgaggt
     1261 ggtgaccaag cacggcggca acgatccgga cgcggccggc gccaaggcgc ttgccgccaa
     1321 gtgcctcgag cgcggcctgc tcatcctcac ctgcggcgtc tacagcgaca cggtgcgcct
     1381 gctcaccccg ctcaccgcct ccgacatggt gctgaacgaa ggactcgaca tcctagagag
     1441 cgccattctc ggcaattgag cccgctcaga cgccggtgaa caggagatcg gccgcgccga
     1501 cgaactcggc gtggcgatcc cgcacattgg cgaccgcctg accaagcctc tggcgcggga
     1561 tgccggccag ttcctgcatc accacgcgat aggccttgcc cgacagcatg agccgtggat
     1621 tgaggcgctt gatcgtctga gcctcacgtt ccgccactac gggtgcgaca accacggtct
     1681 caagcggcga cagcaagtct gactgcagga cgatcaggaa gggaatgcgg ctacgggacg
     1741 agcgatcccc gttgcggcat acgtcgtact gcctgactac cagcggcgcc atccgtcgga
     1801 ccagacgccg ttggcgcgaa cctcttcatt gtaggcttcg atcgccgtcc tgttttcgtc
     1861 cagccaacgc tgcgccgcct gctgggccag agcatgttga agcactgcag aaagattgat
     1921 cccctgggcc cgcgcggcct cgagaagctg cgcgttgacg gacaggttca ctgctttctt
     1981 tcgtgtgcgg cttgtcatgc gcataagcta tgcgcattca tgtgtgaggt caattcaacg
     2041 ttcgagtttc gacgatgccg gacggggctg aacgtgctgc ccggctttgg gcggcgcatg
     2101 caagcggcgc gcatcgcgca tgcctgtcca agctccccgg cggctgctat aaggccgctt
     2161 caccctgagg agttccgcat gccgctgtcc aacatggccg tccgcgatat cgagacggtg
     2221 atccatccct acaccaatct cgcccgccat cgcgaagtcg gcccgaccat cctcaacgag
     2281 ggccgcggca tctatctcta cgacgacagc ggcaagcgct acatcgaggg catggcgggg
     2341 ctctggtgca cggcgctggg ctacggcaac gaagagatcg tcgacgctgc cgcggccgcc
     2401 atgcgtaagg tctccttcac ccatgccttc agcgggaagt cgcatgacgg ggtgatcgag
     2461 ctgtccgaga agctcaagga aatctcgccg gtccccgcct ccaaggtgct gttcggcacc
     2521 tcgggctccg aggccaacga catgcagatc aagctgtcgt ggtactacaa caatgcgcgc
     2581 ggcaagcgcg agaagaagaa gatcatctcg cgcatccggg gctatcatgg cgtcactatc
     2641 gcctcggcct cgctcaccgg gcttccctgg gttcagactg atttcgacct gcccatcgcc
     2701 aatgtgttcc acacctcctg cccgcaccac taccgcttcg gccaggacgg cgagagcgag
     2761 gaggccttcg ccgcccgcat ggcgcaggac ctcgatgacc tcatcatcaa ggaaggcccc
     2821 gacaccatcg ccgccttcat cgccgagccg atcatgggtg cgggcggcgt catcgttccg
     2881 cccgacagct atttccccaa gatcaacgcg gtgctggcga agtttgacgt gcgcttcatc
     2941 gccgatgaag tgatctgcgg cttcgggcgc accggcaagt ggttcggcac cgagaccttc
     3001 ggcatgaagg cccagtccat atccatggcc aaggccatca cctcggccaa tttccccatg
     3061 tcagcgctga ccatcgagga agacctataa cagtccatgc tggacgagag ccgcaaacac
     3121 gccacct
//
LOCUS       DBNGVR010000141         2290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1425758, whole genome shotgun sequence.
ACCESSION   DBNGVR010000141 DBNGVR010000000
VERSION     DBNGVR010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1425758"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="AC16A0_03045"
     CDS             complement(<1..253)
                     /locus_tag="AC16A0_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3104337.1"
                     /translation="MSRLRVHPERHLVTRIGWLRAAVLGANDGIVSTASLIVGVAAAS
                     PARSDVLIAGVAGLVAGAMSMAAGEYVSVSSQSDTEQADL"
     gene            complement(297..371)
                     /locus_tag="AC16A0_03050"
     tRNA            complement(297..371)
                     /locus_tag="AC16A0_03050"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(337..339),aa:Gln,seq:ttg)
     gene            452..528
                     /locus_tag="AC16A0_03055"
     tRNA            452..528
                     /locus_tag="AC16A0_03055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:486..488,aa:Pro,seq:cgg)
     gene            complement(568..1140)
                     /locus_tag="AC16A0_03060"
     CDS             complement(568..1140)
                     /locus_tag="AC16A0_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR3104338.1"
                     /translation="MHNGLLADRIWPAHATGRPARIAALAVIGSLLVAVAAQVSVPMI
                     PVPMTLQTLAVLMVGAAYGARLGAATLALYALQGALGLPVFANLMGGYAVLAGPTGGY
                     ILGFILAAGLVGYLAERGWARTPLRLLAACLAGAAILYVPGLLWLSQFTGAAQVFSVG
                     LFPFLPGDVVKAALVALALPAAQALIGGKS"
     gene            1218..2102
                     /locus_tag="AC16A0_03065"
     CDS             1218..2102
                     /locus_tag="AC16A0_03065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3104339.1"
                     /translation="MTALDFDRNDRAAPGEAEQLTPLVRRILCNNPSAFTFTGTSTFI
                     VGRGRVAVIDPGPDDPAHLAALRHALRGETVTHVLVTHSHADHSPLAARLKAETGATV
                     LAFGAADTASGDALRLDAGIDHGFAPDESLRDGETLSGPGWTLDAVFTPGHMSNHMCF
                     ALREEQSLFSGDHVMFWATSVIAPPEGRMGDYLSSLRKLLERDDKYYHSAHGPGRRDP
                     LPFVRGLLSHRRMREAAILQRLRSGDRDAKEIVAALYADVDPRLHGAAALSVRAHLEN
                     LVEKGQARLTDDGYEALG"
     gene            2147..>2290
                     /locus_tag="AC16A0_03070"
     CDS             2147..>2290
                     /locus_tag="AC16A0_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3104340.1"
                     /translation="MLVDVRQVRKSYATGEGPLQVLQGVDLQLARGESVALTGESGSG
                     KSTL"
BASE COUNT          394 a          782 c          757 g          357 t
ORIGIN      
        1 ccagatcggc ctgctcggta tcggactgcg aactgaccga aacatactcg cccgccgcca
       61 tcgacatggc gccggccacc aggcctgcca cgccggcaat cagcacgtcg ctgcgggccg
      121 gcgaggccgc cgccacgccc acgatcaggc ttgccgtcga gacgatgccg tcattggccc
      181 ccagcaccgc ggcgcgaagc cagccgatgc gggtaacaag atgacgctcg ggatggacgc
      241 gcagtcggct cacgagtcct cgctttcgcc ggtgaccggc cctgtgttgc cggacgtggc
      301 tggggcggga gggatcgaac ctccgcatgg cggaatcaaa atccgctgcc ttaccgcttg
      361 gcgacgcccc aagttccggg gctccgtata acggccccgc tcatgcttgc atagtccgaa
      421 aagcgccgtt ataagcgccg cgaaccaatg acggagtgta gcgcagtctg gtagcgcacc
      481 tgcttcggga gcagggggtc ggaggttcga atcctctcac tccgaccact atgaaacgcg
      541 ggccctgcgc ccgcgttttc ttttctgtca ggacttgccg ccgatcagtg cctgggccgc
      601 cggaagcgcc agggcgacaa gggccgcctt caccacgtcg ccgggcagga aggggaagag
      661 tcccacggag aaaacctgcg ctgcgccggt gaactgcgaa agccagagca ggcccggcac
      721 atagaggatg gctgcgccgg caaggcaggc cgccagcagg cggagcggcg tgcgcgccca
      781 gccgcgttcg gccaggtagc cgaccaggcc tgcggccagg atgaagccca ggatgtagcc
      841 gccggtcggc ccggcaagca ccgcatatcc gcccatgagg ttggcgaaga cgggcaggcc
      901 cagggcgccc tgcagtgcat agagcgccag tgttgccgca cccagccgcg caccataggc
      961 cgcgcccacc atcagcacgg ccagagtctg cagcgtcatc ggcaccggga tcatcggcac
     1021 gctcacctgc gcggccaccg ccacaagcag ggagccgatg acggcaaggg ccgcaatgcg
     1081 ggcaggcctg ccggtggcat gggcgggcca gatacggtcg gcgaggaggc cgttgtgcat
     1141 tttcttctct ctttcgctgc tgtaaggaat ctctccatga cacttatagt tttgtgcatc
     1201 agccgctgca acgccgcatg acggcactcg atttcgaccg caatgacagg gcggccccag
     1261 gcgaggcgga gcagctcacg ccgctggtgc gccgcatcct ctgcaacaat cccagcgcct
     1321 tcaccttcac cggcacatcg accttcatcg tcggccgcgg ccgcgtcgcc gtcatcgatc
     1381 cgggacccga cgatccggcc catcttgcag ccctgcgcca cgcgctccgc ggggaaaccg
     1441 tcacccatgt gctggtgacg cattcgcatg ccgaccactc gccgctcgcg gcaaggctca
     1501 aggcggagac cggcgccacc gtgctcgcct tcggcgccgc cgatacggca tccggggacg
     1561 cgcttcgcct cgatgccggg atcgaccacg gatttgcgcc cgacgaaagc ctgagggatg
     1621 gtgagaccct gtcgggtccg ggttggacgc tcgatgccgt cttcacgccg ggccacatgt
     1681 ccaatcacat gtgcttcgcg ctgcgcgagg agcagtcgct cttctcgggc gaccatgtca
     1741 tgttctgggc caccagcgtc attgcgccgc cggagggccg gatgggcgat tacctcagct
     1801 cgctccgcaa gctgctggag cgggatgaca agtactatca ttccgcccat ggaccgggac
     1861 gccgcgatcc gctgcccttc gtgcggggcc ttctgtctca tcgccgcatg cgcgaagccg
     1921 ccatcctgca gcgcctgcgc agcggggacc gggatgcgaa ggagatcgtc gcggcgctct
     1981 atgcggatgt cgatccgcgc ctgcatggcg ccgcggcgct gtcggtgcgc gcccacttgg
     2041 agaatctcgt cgagaagggc caggcgcggc tcacggatga tggatacgaa gcccttggct
     2101 gaccgcctca catctgccgt gctgcaagtt gcgtaatgga caggccatgc tcgtcgacgt
     2161 tcgccaggtc cgcaagtcat atgccacggg agaagggccg ctgcaggtgc tgcagggcgt
     2221 cgatctccag ttggcgcggg gcgaaagcgt ggcgctcacc ggcgagtcgg gcagcggcaa
     2281 gagcacgctg
//
LOCUS       DBNGVR010000142         1744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3586565, whole genome shotgun sequence.
ACCESSION   DBNGVR010000142 DBNGVR010000000
VERSION     DBNGVR010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3586565"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16A0_03075"
     CDS             complement(<1..202)
                     /locus_tag="AC16A0_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104341.1"
                     /translation="MLRLDVAAGVKPNGKPPVRIFLGTEAGQFRAERVFVWSVINTRD
                     PARVYEIYFMKNLKGYDRTGWKT"
     gene            complement(492..1406)
                     /locus_tag="AC16A0_03080"
     CDS             complement(492..1406)
                     /locus_tag="AC16A0_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3104342.1"
                     /translation="MVDRQASIGERIVGLLPDGPRIALKRGIRGLSEKRKLARADLVV
                     VGHPKSGNTWLRFQLARVYQHKYGLPESVIPDVEILHGLNRSIPQLHMGAYTYIRPII
                     AGPAPAAQLAGKGVVFIVRHPLDIMVSLYLHIQKHALHERKLFNNWPVDLSKTSMAEF
                     TDNPDWGLRPLIGFFNDCLRQHERLEHSMIVSYEDMKAQPAQLLARITALAGGPVSPE
                     EAEEAAAYTSFDKLRQAEIENKFNTTRLRAANPGDPDSFKVRRAKVFGYLDYFSGEVL
                     ERLQAMIDRDLDPRLGYGNSATKDTPGM"
BASE COUNT          342 a          563 c          493 g          346 t
ORIGIN      
        1 cggtcttcca gccggtacgg tcgtatccct tcagattctt catgaagtaa atctcataga
       61 cccgggccgg atcgcgcgtg ttgatcacgg accagacgaa cacccgttcg gcgcgaaact
      121 ggccagcttc cgtccccaaa aagatgcgga ccggcggttt tccgttcggc ttcacgccgg
      181 cggcaacatc aaggcgaagc atctcgggaa agtccctgac gccttccggg cggaccaggt
      241 ccttctggaa ccagtaacgc ggcgtccgat gataccaccg cgtcttgaac tgaccttctt
      301 ttgcgtactt cctagccatt ttcatcacgc cccgtagccc ggcgcctctc agttgtgaat
      361 tcgaagacaa tgacccggcg atagaacacc actccgggcc ggccgcatat tgaccaatat
      421 caattcccca aaccggggcg gcagattccg tctcttaggg cgcaaaccta ggccaaagca
      481 agacgttgcc gctacatacc gggagtatcc tttgtcgctg agttcccata acccagccgc
      541 ggatcaagat cacgatcaat catggcctga aggcgctcca gcacctcgcc gctgaagtag
      601 tcaagatagc caaaaacctt ggcgcggcgc accttgaagc tgtccgggtc gcccggattt
      661 gccgcgcgaa ggcgcgtcgt attgaatttg ttctcgatct ctgcctgccg aagcttgtcg
      721 aatgacgtat aggccgccgc ttcctctgct tcctccggac tgaccgggcc tccggccaag
      781 gcggtgatgc gggccagaag ctgcgccggc tgcgccttca tgtcttcgta gcttacgatc
      841 atggagtgct ccagccgttc atgctgacgc aggcagtcgt tgaagaagcc gatgagcggc
      901 cgcagccccc aatccggatt gtcggtgaac tcggccatgc tcgtcttgct gagatcgacc
      961 ggccagttgt tgaagagctt gcgctcgtgc agggcatgtt tctggatgtg aaggtagagc
     1021 gagaccatga tgtccagcgg atggcgcacg ataaacacga cgcccttgcc agccagctgt
     1081 gccgccggcg caggaccagc gatgatcggc cggatatagg tataggcgcc catgtgcagc
     1141 tgcgggatgc tgcgattgag gccatgcagg atttccacat ccgggatcac ggattcgggc
     1201 agaccatatt tgtgttggta gacgcgcgcc agctggaaac gcaaccaggt attgccgctc
     1261 tttggatggc cgactacaac gagatcggca cgcgcgagct tgcgcttttc ggacaggcct
     1321 cgaatccccc gcttcagcgc aatgcgtggc ccgtccggca gcaacccgac aatccgttcg
     1381 ccaatactgg cctgccgatc gaccatccta tccacctttg tccctcagcc gccttgagcc
     1441 gcgacccgtc gccggttcgg ttggcggcac catcgggaaa cacgccgtgc caccgcacgc
     1501 tcccaccggg cgccgccttt tatcggcaag cggtcaaccg aggcaatcac tgttttgggg
     1561 ttctgcgcgc agcgcgacac ctaagttcca gggccttcac gaagcccttg ccaggcccgc
     1621 gccagtatgt cgccaacaat gaccacctcg ccttcgaact gcgacaggag tccgaggatg
     1681 aactccagtt ccgtctcccg gctatggccc ggcgaatagg cattcgggcc cgatgccgcc
     1741 cagg
//
LOCUS       DBNGVR010000143         4094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3586621, whole genome shotgun sequence.
ACCESSION   DBNGVR010000143 DBNGVR010000000
VERSION     DBNGVR010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3586621"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="AC16A0_03085"
     CDS             <1..1900
                     /locus_tag="AC16A0_03085"
                     /inference="COORDINATES: protein motif:HMM:NF044955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HIR3104343.1"
                     /translation="ALMLAFCLLLGFAAEALAEEKITSFISDVTVNADASLEVRERIS
                     VNAEGDEIRRGILRDFPTIYTDRNGVRVKVGFEVIEVLRNGRSEPFGLESLSNGTRIR
                     IGNKDVLIGSGIHVYEIVYRTTRQLGFFETYDELYWNVTGNGWTFAIDRAAVTIRLPP
                     GAEIQQSAGYTGPQGADGRDFRVLNGNGSVFEAETTRRLEPYEGFTVAVAWQKGIVMP
                     PSEADKWRWWIGDNAGIFALVASLLASGFYYFSAWNKVGRDPPKGTIIPLFRPPAGLS
                     PAGARYLREYGLDDRGFAAALVGLAVKKHLKIKDEDGEFAITPLNRSGGGDPLTPAEG
                     ALFKALPSGELKLKQSNHQMVRNARSVLKDALEAEYEGSAFVRNIGAFVKGAIISAVG
                     LVLGALLMPPEDGFAGLFLVGWMSIWWGVVLTFLWFTLKGLFQGKGIASRVGSVFMLL
                     FLVPFVGGGVMAPLGMLMGAGSPRLYLLIGTAVLLGVMNLVFFYLLRAPTLPGRKLLD
                     EIEGFRLYMTTAEEERLKVLHPPEKTPELFERYLPYALALDCENEWNAKFASVLAAAA
                     MAGAAQPGWYSGRNWDAGRTGSFTDSLGSSLSSSVASASTAPGSRSGSGGGGSSGGGG
                     GGGGGSGW"
     gene            complement(1901..3712)
                     /locus_tag="AC16A0_03090"
     CDS             complement(1901..3712)
                     /locus_tag="AC16A0_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3104344.1"
                     /translation="MAQILLHLRDIKLTFGGKPVLEGAELAVSEGERIALVGRNGSGK
                     STLLKIAAGLVDYDKGSRWLQPGTTLRYLPQEPDLAGFATVMDYVKAGLGPTDDPHRC
                     VSLLRDLGLSGAEDPARLSGGEIRRAAIARTLAPAPDILLLDEPTNHLDLPAIEWLEA
                     ELKSLRSAMVLISHDRRFLEALTNRTVWLDRGASRRLDRGFAQFEAWRDEILAKEETE
                     QALLAQRIATEEHWMRYGVTARRKRNMRRVDLLAKLRKDRREHVGALGAVRMAASAGD
                     LSGKLVAEAKAMSKSFGDRVIVDKLDLLVMRGDRLGIVGPNGAGKTTLIKLITGALAP
                     DAGLIRLGTGLQTVMLDQSRQSLDPASTLADVITDGRGETVTINGETRHVVSYMKDFL
                     FRPEQARTPVSVLSGGERARLILARELAKPSNLLVLDEPTNDLDLETLDMLQEMLADY
                     QGTILLVSHDRDFLDRVASSVLMSEGEGRWTEYAGGYTDMVAQRGAGVAARKAKAVTS
                     PKAAAASPAAAPAQGQRKLSFKEKHALETLPGLMQKLESEAAALEQRLADPGYFGRDA
                     KGFDADIKRLAAIRDELARAEEQWLELEMKREELEGA"
     gene            complement(3726..>4094)
                     /locus_tag="AC16A0_03095"
     CDS             complement(3726..>4094)
                     /locus_tag="AC16A0_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3104345.1"
                     /translation="IAGLARRKPAEISGGERQRVAIARALLRDRPILLLDEPFAALGP
                     ALRGDMLDLLMTLQKDHKLTVLMVTHLPDDALQAADLTAFVDHGEIVALRPTAELLAS
                     TDVPGLESYLGALEPARPMR"
BASE COUNT          636 a         1317 c         1420 g          721 t
ORIGIN      
        1 cgccctgatg ctggccttct gcctcctcct cggcttcgcc gccgaggccc tggccgaaga
       61 gaagatcacc tccttcatca gcgacgtgac ggtgaatgcc gatgcgtcgc tcgaggtgcg
      121 cgagcgaatc tcggtcaatg cggaaggcga cgagatccgg cgtggaatcc tgcgtgactt
      181 tccgaccatc tataccgacc gcaacggcgt cagggtgaag gtcggcttcg aggtgatcga
      241 ggtgctgcgc aacgggcgct cggagccctt cgggctcgag tccctcagca acggcacgcg
      301 tatccgcatc ggcaacaagg atgtcctgat cggcagcggc attcatgtct atgagatcgt
      361 ctatcgcacc acgcggcagc tgggcttctt cgagacctac gacgaactct actggaacgt
      421 gacgggaaat ggctggacct ttgccatcga ccgcgccgcc gtgaccatcc ggctgccacc
      481 gggcgcggag atccagcaat ccgcgggtta caccgggccg cagggcgccg atggcaggga
      541 cttccgcgtt ctcaacggca atggaagcgt cttcgaggcg gaaaccacgc ggcgcctcga
      601 accctacgag ggcttcaccg ttgcggttgc ctggcagaag ggcatcgtca tgccgcccag
      661 cgaagccgac aagtggcgct ggtggatcgg cgacaatgcc gggatctttg ccctggtcgc
      721 atctcttctg gcgtccggat tctactattt ctcggcgtgg aacaaggtgg gccgcgatcc
      781 gcccaagggc acgatcatcc cgctcttccg cccgccggca ggcctcagcc ccgccggtgc
      841 gcgctacctg cgcgaatacg gtctcgacga tcgcggcttt gcggcggcgc tcgtcgggct
      901 ggcggtgaag aagcatctga agatcaagga cgaggacggc gagttcgcga tcacgccgct
      961 caaccgctcg ggcggcggcg atccgctgac gccggcggaa ggcgccctgt tcaaggccct
     1021 gccatcgggc gagctgaagc tgaagcagtc caatcaccag atggtgcgca atgcccggag
     1081 cgtactcaag gatgcgctgg aggcggagta tgaaggcagc gcctttgtcc gcaacatcgg
     1141 tgccttcgtg aagggtgcga tcatctcggc ggtgggcctg gtccttggcg cgctcctcat
     1201 gccgccggaa gatggcttcg ccgggctctt tctcgtcggc tggatgagca tctggtgggg
     1261 cgtggtcctg accttcctgt ggttcacgct gaagggcctg ttccagggca agggcatcgc
     1321 cagccgcgtg ggctcggtct tcatgctgct gttcctggtg cccttcgtcg gcggtggcgt
     1381 catggcgccg ctgggcatgc tcatgggggc gggatcgccg cggctttacc tcctcatcgg
     1441 aacggcggtg ctgctcggcg tcatgaacct cgtcttcttc tatctcctgc gtgcgcccac
     1501 tctgccgggc cgcaagctgc tggacgagat cgaaggcttc cgcctctaca tgacgacagc
     1561 ggaagaagaa cgcctgaagg tcctgcatcc gccggagaag acgcctgagc tcttcgagcg
     1621 ctatcttccc tatgccctgg cactggactg cgagaacgaa tggaatgcga agttcgcatc
     1681 ggtcctggct gccgcggcca tggccggcgc ggcgcagccg ggctggtata gcgggcgcaa
     1741 ctgggatgcc ggacggaccg ggagcttcac cgacagcctg ggcagcagcc tgtcatccag
     1801 cgtggcctcg gcctcgacgg cgcccggcag ccggtcggga tcgggtggcg gcggatcgtc
     1861 gggcggcggt ggtggcggcg gtggtggatc gggctggtag tcaggcgcct tccagctcct
     1921 cgcgcttcat ctcgagttcc agccactgtt cttcggcccg tgcgagctcg tcgcggatcg
     1981 cggccagccg cttgatgtcg gcatcgaaac ccttggcatc gcggccgaaa tagcccggat
     2041 cggcaaggcg ctgttcgagc gccgccgcct cggactcgag cttctgcatg aggccaggca
     2101 gggtttccag cgcatgcttc tccttgaagg agagcttgcg ttgcccctgt gccggcgccg
     2161 cggccggact cgccgctgcg gcctttggag aggtcacggc cttcgccttc cgggccgcca
     2221 cgccagcacc gcgctgagcc accatgtcgg tatagccgcc ggcatattcg gtccagcggc
     2281 cctcgccttc cgacatcagg acggaagagg cgacgcgatc cagaaagtcg cggtcatggc
     2341 tcaccagcag gatggtaccc tgatagtcgg ccagcatctc ctgcagcatg tcgagcgtct
     2401 cgaggtcgag atcattggtc ggctcgtcaa gcaccagcag gttggagggc ttggccagtt
     2461 cgcgcgccag gatgaggcgc gcgcgctcgc cgccggagag caccgagacc ggcgtccgcg
     2521 cctgctcggg acgaaagagg aagtctttca tgtagcttac cacatggcgc gtctcgccat
     2581 tgatggtgac ggtctcgccg cgcccgtcgg tgatgacatc ggccagcgtg ctggcgggat
     2641 cgaggctctg ccggctctgg tcgagcatta cggtctgcag cccggtgccg aggcggatca
     2701 acccggcatc gggcgccagc gcgccggtga tgagcttgat cagcgtcgtc ttgcccgcgc
     2761 cattgggtcc gacgatgccg agccggtcgc cacgcatgac caggaggtcg agcttgtcga
     2821 cgatcacccg gtcgccgaag gatttcgaca tggccttggc ctcggccacc agcttgccgg
     2881 agagatcgcc ggccgaggct gccatgcgga cggcgccgag tgcgcccaca tgctcccgcc
     2941 ggtccttgcg cagcttggca agcaggtcca cgcggcgcat gttgcgcttg cgccgcgcgg
     3001 tcacgccata gcgcatccag tgttcttcgg tggcgatgcg ctgggccagc aacgcctgtt
     3061 cggtttcttc cttcgccagg atctcgtcac gccaggcctc gaactgggcg aagccgcggt
     3121 caaggcggcg cgatgcgccg cggtccagcc agacggtgcg gttggtcagc gcctcgagaa
     3181 agcggcgatc atggctgatc agcaccatgg cgctgcgcag cgacttcagt tccgcttcca
     3241 gccattcgat ggcgggaagg tcgagatggt tggtgggctc gtcgagcagc aggatatcgg
     3301 gggccggcgc cagcgtgcgc gcgatggcgg cgcggcggat ctcgccgccc gacagtcgcg
     3361 cgggatcctc cgcgccgctg aggcccagat cgcgcagcag gctgacgcag cggtgtggat
     3421 cgtcggtcgg gccgagaccc gccttcacat agtccatcac ggtggcgaag cccgcgagat
     3481 ccggctcctg cggcagatat cgcagcgtgg tgccgggctg cagccagcgg ctgcccttgt
     3541 cgtagtcgac gagaccggcg gcgatcttga gcagcgtcga cttgcccgag ccgttgcgcc
     3601 ccaccagggc gatccgctcg ccctcggaca cggcaagctc ggcgccttcg agcaccggct
     3661 tgccgccgaa ggtcagtttg atgtcgcgaa gatgaagcag gatctgggcc atggctgcgg
     3721 gcggattagc gcatcggccg cgccggttcc agtgcgccga gataggattc gaggccgggc
     3781 acgtcggtgc tggcgagaag ttctgcggtg ggccggagtg cgacgatctc gccatgatcg
     3841 acgaaggctg tgagatcggc cgcctgcaag gcatcatcgg gcaggtgcgt caccatgagg
     3901 accgtcagct tgtggtcctt ctgcagcgtc atcagcagat cgagcatgtc gccgcgcagt
     3961 gccgggccga gggcggcgaa gggttcgtcc agcaacagga tcgggcggtc gcgcagcagg
     4021 gcgcgcgcga tggcgacgcg ctggcgctcg ccgccggaga tctcggcggg cttgcgccgg
     4081 gcgagacctg cgat
//
LOCUS       DBNGVR010000144         3420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4766105, whole genome shotgun sequence.
ACCESSION   DBNGVR010000144 DBNGVR010000000
VERSION     DBNGVR010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4766105"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..1453
                     /locus_tag="AC16A0_03100"
     CDS             404..1453
                     /locus_tag="AC16A0_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104346.1"
                     /translation="MASITLEKITKAWGAIKGVDAIDLEIRDKEFMVFLGPSGCGKTT
                     TMRMIAGLEDPTEGNIIIDGEKVNEVDARDRDVAMVFQSYALYPNMTIYENIRFPLRM
                     RGVPKDQHDRLVREAADIVELGELLDRKPKALSGGQRQRAALARAVVRHPQVFLMDEP
                     LSNLDAKLRLSMRGQLKHIQRKLQTTTVYVTHDQIEAMTLADRIAIMDKGKIQQLGTP
                     DEVYNDPANIFVAGFIGSTPMNLIKGDLENGTFVAPGTHVAKAGKGSRKNVVLGIRPE
                     DVSVVAKSKGSFTAPLFTLEPTGDQTLAAARNDAQMVVARAARDFRQEIGSDISFAFA
                     PDRVYLFDAASGARI"
     gene            complement(1457..2566)
                     /locus_tag="AC16A0_03105"
     CDS             complement(1457..2566)
                     /locus_tag="AC16A0_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HIR3104347.1"
                     /translation="MKSRAAVAFAAGQPLEIVEVDVEGPKAGEVLVEIKATGVCHTDD
                     FTISGADPEGLFPAIMGHEGAGVVVEVGPGVTSLKPGDHVIPLYTPECRECEYCLNPK
                     TNLCQKIRATQGKGLMPDGTSRFSYKGKPVHHYMGCSTFSNYSVLPEIALAKVRRDAP
                     FDKICYIGCGVTTGVGAVINTAKVEVGSNCVVFGLGGIGLNVIQGLRLAGASMIVGVD
                     LNDDREAWGRRFGMTHFVNPSKVTGDLVAHLVEMTGGGADYTFECIGNVKVMRIALEC
                     AHKGWGESIIIGVAGAGQEISTRPFQLVTGRSWRGTAFGGARGRTDVPKIVDWYMNGR
                     IEIDPMITHVLPLEKINEAFDLMHQGKSIRSVITY"
     gene            complement(2688..3368)
                     /locus_tag="AC16A0_03110"
     CDS             complement(2688..3368)
                     /locus_tag="AC16A0_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR3104348.1"
                     /translation="MHIEPGVVDGAKIVLSYATAAASFGLAAKLAMDTIRKDGGMAAL
                     LTRSVITTALVFSFFELLPHYPVGVSEVHLILGSTLFLIFGAGPAAIGLALGLLLQGV
                     FLAPFDLPQYGMNVTTLLVPLYAISLLARRIIPANTPYVDLTYRQALALSTSYQGGIV
                     AWVAFWALYGHGFTAENIGNILAFGGAYMLVILLEPLLDLAVLAGAKTIRQLKNSVLV
                     QQRVYQAA"
BASE COUNT          697 a         1046 c         1074 g          603 t
ORIGIN      
        1 atatccatct tcacgtgcgt gcgtcccttc accatgactg tccaccggcc gcagtcggca
       61 gcgcatcccg gaagcgctct tggggcaggc gcagatcaca caacggcgga tgagcgggca
      121 ccgaatacgg gcctccaatt ctgaagccaa ttagatactt ttgacctcat ggaagtcaat
      181 aagtaatatt atgcagaatt gcatcgtaaa cactgggtca tcgacgataa ccgatggtgc
      241 gacgcacaaa attctgcggc gcagcatgga agttaatggt cctgatggct tccctttgtc
      301 gagaaatctg acacaaatgg actccaatct tgaaagtgtg tcagttctat gggactgatc
      361 gcgcaacgaa cccggcaacc ggggaagtgg accgggaggg cgaatggcgt cgattacgct
      421 ggagaagatc accaaggcct ggggtgcgat caagggcgtc gatgcgatcg acctcgagat
      481 ccgcgacaag gagttcatgg ttttcctcgg tccctcgggc tgcggcaaaa ccacgaccat
      541 gcgaatgatt gcgggcctcg aggatccgac cgagggcaac atcatcattg acggcgagaa
      601 ggtgaatgag gtcgatgcgc gcgaccgcga cgtggccatg gtgttccaga gctatgcgct
      661 ctaccccaac atgacgatct acgagaacat ccgctttccg ctgcgcatgc gaggtgtccc
      721 caaggatcag catgaccggc tggtgcgtga agccgccgac atcgtggaac tcggagaatt
      781 gctggaccgc aagcccaagg cgctctccgg cggccagcgc cagcgcgcgg cactggcccg
      841 cgccgttgtg cgccacccgc aggtctttct catggacgag ccgctgtcaa acctcgatgc
      901 caagctgcgc ctgtccatgc gcggccagct caagcatatc cagcgcaagc tgcagacaac
      961 gaccgtctat gtgactcatg accagatcga ggccatgacg ctggccgacc gcatcgccat
     1021 catggacaag ggaaagatcc agcagctggg cacccccgac gaggtctaca acgatcctgc
     1081 gaacatcttc gtggcgggct tcatcggatc cacgccgatg aacctgatca agggcgacct
     1141 cgagaatggg acattcgtcg cgcccggcac gcatgtcgcc aaggcgggca agggcagccg
     1201 caagaacgtg gtgctgggca tccgtcccga ggacgtcagc gtcgtcgcca agtcgaaggg
     1261 cagcttcacg gcgccgctct tcacgctgga gccgaccggc gatcagactc ttgccgccgc
     1321 gcgcaacgac gcgcagatgg tggtggcccg tgccgcgcgc gacttccgcc aggagattgg
     1381 cagcgacatc tccttcgcct tcgcgcccga tcgggtctat ctcttcgacg ccgcgtcggg
     1441 cgcacgcatc tgactctcag taggtgatca ctgaccggat cgacttgcct tgatgcatca
     1501 ggtcgaaggc ctcgttgatc ttctcgagcg gcaggacgtg cgtgatcatc ggatcgatct
     1561 cgatcctgcc gttcatgtac cagtcgacga tcttgggcac gtcggtgcgg ccccgcgcgc
     1621 caccaaaggc cgtgccgcgc catgaccgtc cggtaaccag ctggaagggc cgtgtggaga
     1681 tctcctgccc ggcgccggcc acgccgatga tgatcgactc gccccagccc ttgtgcgcgc
     1741 attcgagtgc gatgcgcatg accttcacgt tgccgatgca ttcgaaggtg tagtcggcgc
     1801 cgccgccggt catttcgaca agatgcgcca cgagatcgcc cgtcaccttc gacgggttga
     1861 cgaagtgggt cataccgaag cgccggcccc aggcctcgcg gtcatcgttg agatcgacgc
     1921 ccacgatcat gctggcaccg gcaagccgca ggccctggat cacgttgagg ccaatgccgc
     1981 ccagtccgaa gaccacgcag ttggaaccca cctccacctt ggccgtgttg atcaccgcgc
     2041 ccacgccggt ggtgacgccg cagccgatgt agcagatctt gtcgaagggc gcgtcgcggc
     2101 ggaccttggc caacgcaatc tcgggcagca ccgagtaatt cgagaaggtg gagcagccca
     2161 tgtagtgatg gacgggcttg cccttgtagg agaatcgcga agtgccgtcc ggcatcagcc
     2221 ccttgccctg ggtcgcccgg atcttctggc agaggttggt cttggggttc aggcagtact
     2281 cgcactcgcg gcactccggc gtatagagcg gaatgacgtg gtcgccgggc ttcagcgagg
     2341 taacgcccgg cccgacctcg accacgacac ccgcgccttc atggcccatg atcgccggaa
     2401 acagcccctc cggatcggcg cccgagatgg tgaaatcgtc cgtgtggcag acgccggtgg
     2461 ccttgatctc gaccaggact tcgccggcct tcggcccctc cacgtcgacc tcgacgattt
     2521 caagcggctg accagcggca aaggcgacgg cggcacgaga tttcatggca agggatcctc
     2581 aggtcatcga tgagagcttc cggcggccgc atcgcgctga ggcctggtgc cctgtggtag
     2641 atgaagaagg cgcggcggcc aagacagccg ccgcgccatg atcgtattca ggccgcctga
     2701 tagacgcgct gctgtaccag caccgagttc ttcagctgac ggattgtctt ggcaccggcg
     2761 agaacggcca ggtcgagcag cggctcaagc aggatgacca gcatataggc gccgccgaag
     2821 gcaaggatgt ttccgatgtt ctcggccgtg aagccgtggc catacaaagc ccagaaggca
     2881 acccaggcga cgatgccgcc ctgatagctg gtcgacaggg caagggcctg gcggtaggtc
     2941 agatcgacgt agggcgtgtt cgcgggaatg atgcgtcttg caagtaggct gatggcgtag
     3001 agcggcacca gcagggtcgt gacgttcatg ccatattgcg ggagatcgaa gggcgcgagg
     3061 aacacgccct gaagcagaag gccgagggcg aggccgatcg ctgccgggcc tgcgccaaag
     3121 atgaggaaga gtgtcgatcc caggatcaaa tgaacctctg acacgccgac gggataatgc
     3181 ggcagcaatt caaagaatga aaaaaccagc gccgtggtga tgacggagcg ggtcagcagg
     3241 gcggccattc cgccgtcctt gcggatggtg tccatggcca atttggccgc aaggccaaag
     3301 gaagccgctg ccgtggcata gctcagaacg atctttgcgc cgtcgacgac gccaggttcg
     3361 atatgcatga ttcaagtcct ttctgcaccc atcgtgcagt ccttggttgt ctggcgcagg
//
LOCUS       DBNGVR010000145         4694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2800306, whole genome shotgun sequence.
ACCESSION   DBNGVR010000145 DBNGVR010000000
VERSION     DBNGVR010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2800306"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="AC16A0_03115"
     CDS             complement(<1..1062)
                     /locus_tag="AC16A0_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104349.1"
                     /translation="MSRTAGPFARMGLPGREAERGLLLLADDPAAFDAMQPLADALAR
                     RDSRINLLSASAVAATAKWLQSHLAGVRQVPLPYGNRVSGDLYLRRLNIRTVAFLDPS
                     RATASPALLRSLKRQAISIVTLTASNARGVEPDAPAVRDSEQVILIDDAAANGASGAM
                     THAAAADFLAALMARDLKPLRQERSLLAAPARALAGMTRSAKNRPMIDWRFRRFATLA
                     DLKRRLGHPATILCLGNGPSCEDPALAAIAHDALFRVNHSWQARQRFNRPQVVFTGGR
                     PTMRAVTGAIFGLQSLEAEVRLASARGLNPMVGATEFFNVNDLTDSIRRFDWGHLRPT
                     NGASMLAAAVALSPQRLVIA"
     gene            complement(1059..2147)
                     /locus_tag="AC16A0_03120"
     CDS             complement(1059..2147)
                     /locus_tag="AC16A0_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HIR3104350.1"
                     /translation="MTASPVPRVWLLIDDRPGHRTQVEGLARMMGWSAEDRILSFNFL
                     NYIPNPILGSSLLSLRGISADSLRPPFPDVVVGMGRRVIPVARWIKRQSGGRSRIVML
                     GRKAGGASRNSDLYVSCSHFGSFPGPRLFELAVPPTKVDAESLAAARNARPDPLDGLR
                     HPRVLLLVGGPTAQHEFTADFAWRMANLVVRSVESLGGDLAIVTSRRTPADAIAAMRE
                     AAPAAHFHEWERARVDNPYLSYLAAADALVVTGESESMLAEGAATGLPLTIFPLQPKP
                     PGLKARLIAAIHRMALDRGLRADVCRYFFDRGWLAPPRDLDRLHRAMVDHGLARLFES
                     GVSLDAPDQNPDLDRLKQRIGELLDQPS"
     gene            2274..3029
                     /locus_tag="AC16A0_03125"
     CDS             2274..3029
                     /locus_tag="AC16A0_03125"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR3104351.1"
                     /translation="MRTLVLMRHGATLWGEENRFAGWGDTPLSETGMKEARKAAATLV
                     RSGLSFDIGYTSCLRRAQQTLDIALDEMQQRDLPVRREWRLNERHYGALQGETRSAMI
                     DRYGNSHVVEWRRSFDAIPPALTDDDPRWQEQLDRLPQIPIADQPRTESMAQAADRIA
                     PLWHEAIRPDLAAGKQLLVVAHTSALRGLIRLIEGLSDKEAADFRIATAIPRVYVMDE
                     NFKPIRTMDLSEGTAASIRYWVTALKPRRIGWI"
     gene            3133..>4694
                     /locus_tag="AC16A0_03130"
     CDS             3133..>4694
                     /locus_tag="AC16A0_03130"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104352.1"
                     /translation="MKRVFSIFFRAEDTRPFLVLFCLLLGGVAEAVGISTLLPVATAI
                     AGGDSVGSSRLNVTVREAISWFGLTPNLSTMVFIVMSMIVFKAILSFAALSYAGVSSA
                     RVSNNLRKRLISAIFEARWSFYAEQSGGRFANAISNDCGRSGDAYALAAQVVAYSVQA
                     VMYAIVGILVDPRIAIVGLLFGLIVTLLTSRLVRVTRKAGFKQTDSTATLTVLMVDML
                     NNIKPLKTMERYDAMLKSLSGTLRRLKRSLVVRQLSHHGLNQLSDALIAVIVAGGIYF
                     ANSYWSVPLPELAVSGMVFYQIIASISKLQKFNMIAAQVESAYVRATELIALAESNRE
                     VNAGKALPEIGNGCRFENVSFSHGSTPILHDVDLEFPTNSITVLSGSSGAGKTTIIDL
                     LIGLNRPTRGRIMIGSTALTDIDVRAWRREIGYVPQELSLLHASIRENISLNDTSISD
                     AQIVEALTLAGAGEFLANLPNGLDTDVGEMGGKLSGGQRQRISLARALVTRPKVLILD
                     EVTSALDGRTEA"
BASE COUNT          901 a         1486 c         1495 g          812 t
ORIGIN      
        1 ggcgatcacc agccgttgcg gcgacagggc caccgcggcg gcaagcatgc tggcgccatt
       61 ggtcgggcgc agatgacccc agtcaaagcg ccggatgctg tcggtaaggt cgttgacatt
      121 gaagaactct gtcgccccca ccattgggtt caaaccccgc gcggacgcga ggcgcacttc
      181 tgcctcaagg ctctggagcc cgaaaatggc accggttact gcacgcatgg tgggacggcc
      241 gccggtgaac accacctgcg ggcggttgaa ccgctgtcgc gcctgccagg agtgattgac
      301 gcggaacagc gcatcatggg caattgcggc gagcgccgga tcctcgcagg acggaccgtt
      361 gccgaggcac aagatggtcg ccggatggcc aagccgcctc ttgaggtccg cgagcgtcgc
      421 aaaccggcgg aagcgccagt cgatcattgg cctgtttttg gcagagcggg tcattccagc
      481 cagcgcccgg gcaggtgcgg ccagcagtga cctctcttgt ctgaggggtt tcagatcgcg
      541 ggccatcagg gcggcaagaa agtccgcggc cgccgcatgc gtcatggctc cagacgcgcc
      601 gttcgccgcg gcgtcgtcga tcaggatgac ctgctcgctg tccctcacgg ctggcgcatc
      661 cggctccacg ccacgggcgt ttgacgcagt cagggtgacg atggagatcg cctggcgctt
      721 caagctccga agcaaggcag gcgacgccgt cgcccgggac ggatcgagaa aggcgaccgt
      781 ccgaatgttg agacggcgaa ggtagagatc gcctgaaact cggtttccgt aaggcaaggg
      841 cacctgccgc actccggcca gatgactttg cagccacttc gccgtcgcag ccactgcgct
      901 ggctgacagc aggttgatgc ggctatcccg cctggcaagc gcgtccgcca gtggctgcat
      961 ggcgtcgaag gcggcaggat catccgccag gaggagcagg ccacgctctg cttcccttcc
     1021 cggcagcccc atgcgggcaa agggtccggc ggtcctgctc acgatggctg atccagaagt
     1081 tccccaatcc gctgcttcag acggtcgagg tctggattct ggtcgggcgc atccaatgac
     1141 accccgctct cgaacaaccg ggccagtccg tggtcaacca tggcgcgatg aaggcggtcg
     1201 aggtcgcgcg gcggcgccag ccagccacgg tcgaaaaaat agcgacagac atcggcgcgc
     1261 aaaccccggt caagggccat ccggtggatg gctgcaatga ggcgcgcctt cagcccgggc
     1321 ggctttggct gaagcgggaa gatggtaagg ggcaggccgg tcgccgcccc ttcggcaagc
     1381 atcgattcgc tttcgccggt cacgaccagg gcatccgcag ccgcgaggta gctgagatag
     1441 ggattatcga cccgtgcgcg ctcccattca tggaaatggg ccgcaggcgc tgcttcccgc
     1501 atggccgcaa tggcgtctgc gggcgtgcgg cggctggtga caatggcgag gtcgccgccc
     1561 aggctttcca cggaacggac gaccagattg gccatccgcc aggcgaaatc cgccgtgaac
     1621 tcatgttgtg ctgtcggacc gcccacgaga agcagaaccc ggggatgccg caggccatcc
     1681 aggggatcgg gccgcgcatt gcgggcggcg gcgagacttt cagcatcgac cttggtgggt
     1741 ggcacggcca gctcgaagag ccggggaccg gggaaggatc cgaaatgact gcagctgacg
     1801 taaaggtcag aatttctgct cgcgccaccc gccttgcgtc caagcatgac gatgcggctg
     1861 cggccgccgc tctggcgctt gatccaacgg gccaccggaa tgacgcggcg gcccatgccc
     1921 accaccacgt cggggaacgg cgggcgcagg ctgtccgcac tgatcccgcg gagcgacaga
     1981 aggctgctac ccagaatcgg attcgggata taattcaaaa agttaaagga caggatccga
     2041 tcctctgcag accagcccat catgcgggcg agcccctcca cctgggttcg gtggccaggg
     2101 cggtcgtcaa tcagcagcca gacgcgcggc acaggtgaag ccgtcatgca gggcagctcg
     2161 cgcggtctcg ggtgggatgc aacatgcggt cgctgtttgg accggtacag ggcggttttc
     2221 aagagcggcg agatggtcta acacgcgccc gacacagcac agaactcagg cggatgcgaa
     2281 cacttgtctt gatgcgccat ggcgccaccc tgtggggtga agagaaccgc tttgccggtt
     2341 ggggcgacac cccgctcagc gagaccggca tgaaggaagc gcgcaaagcc gccgctacgc
     2401 tcgtccgcag tggactctcc ttcgacatcg gctacacctc ctgcctgcgc cgggcccagc
     2461 agacgctcga catcgcgctg gacgagatgc agcagcgcga cctcccggtc cgccgcgaat
     2521 ggcgcctcaa cgaaagacac tacggcgcgc tgcagggcga gacacggtcg gcgatgatcg
     2581 atcgctacgg caacagccat gtggttgaat ggcggcgctc cttcgatgcc ataccgccag
     2641 cgctcacgga tgacgacccg cgctggcagg aacagctgga ccgtctgcct cagattccga
     2701 tcgccgacca gcctcgtacc gaaagcatgg cacaggccgc ggatcgaatc gcaccattgt
     2761 ggcacgaggc aatcaggccc gacctcgctg ccggcaagca attgcttgtg gtcgcccata
     2821 ccagcgcgct gcggggactg atccgtctca tcgaaggcct gagcgacaag gaagccgcgg
     2881 acttccgcat tgcgaccgct attccgagag tctatgtgat ggacgagaat ttcaagccga
     2941 tcagaaccat ggatctctcg gaaggcactg ccgcgtcgat ccggtactgg gtcacggccc
     3001 tgaaaccgcg ccgcatcggt tggatctagt ggggccttca atcctgacct gcgccgtcaa
     3061 gcttgagttc cccacctcgc ttctctattg ctgcgggccc aggccctccg acgaaagctc
     3121 agcaccggca gcatgaagcg cgttttcagc attttcttcc gggcggaaga cacgagaccc
     3181 tttctcgtcc tgttctgcct ccttctgggc ggggtggccg aggcagtcgg catcagcacg
     3241 ctgctaccgg tcgcgacggc catcgcgggc ggagacagcg tcggctcgtc ccgcctcaat
     3301 gtcaccgtgc gcgaggccat cagttggttc ggactgacgc caaacctgtc caccatggtc
     3361 ttcatcgtga tgagcatgat cgtcttcaag gccatactct cgttcgcagc gctgtcatat
     3421 gccggggtct ccagtgcccg ggtctccaac aatctgcgca agcggttgat cagcgccatc
     3481 ttcgaggcgc gctggagctt ctatgccgaa cagagcggcg gacggttcgc caacgcaatc
     3541 agcaatgatt gcggccggtc gggcgatgcc tatgcgctcg ccgcccaggt tgttgcctac
     3601 tcggtacagg ccgtcatgta tgcaatcgtc ggaatcctgg tcgatccgcg cattgcgatc
     3661 gttggattgc tgttcggcct catcgtcacg ctgttgacca gcaggctggt gcgcgtcacc
     3721 cgcaaagccg gtttcaagca gactgacagc acggcgacgc tgacggtgct gatggtcgac
     3781 atgctgaaca acatcaagcc gctcaagacc atggagcgct acgatgcgat gctgaagtcc
     3841 ctgtccggga cactgaggcg cctgaagcgc tcactcgttg tgcggcagtt gtctcatcat
     3901 ggactcaacc agctgagcga cgcgctgatc gcggtcatcg ttgccggcgg aatctacttt
     3961 gccaacagct actggtcggt tccgctgccc gaactggccg tgagcggaat ggtcttctac
     4021 cagatcattg cctcgatctc gaaactccag aagttcaaca tgatcgctgc acaagtggaa
     4081 agcgcatatg tgcgggcgac ggaactcatc gccctggcgg aaagcaaccg cgaggtgaat
     4141 gccggcaagg ccctgccgga gatcggcaat ggttgccggt tcgagaacgt cagcttctcg
     4201 cacggcagca cgccgatcct gcatgatgtc gatctggagt ttccaaccaa cagcataacg
     4261 gttctgagcg gttcatctgg agcgggcaag accacaatca ttgatctgct gatcgggctc
     4321 aaccgtccga ccagaggtcg catcatgatc ggatcgacgg ccctcaccga catcgatgtc
     4381 cgagcctggc gccgcgagat cggctatgtg ccgcaggagc tgagcctgct gcacgccagc
     4441 atccgggaaa acatctcgct gaatgacacg tcgatttcag atgcgcagat cgtggaagcg
     4501 ctcacccttg ccggcgctgg tgaatttctc gccaatctcc ccaacggtct cgacaccgac
     4561 gtcggggaaa tgggcggcaa gctgtccggc gggcagcgcc aacgcatttc tctggcgcgc
     4621 gccctggtca ccaggcccaa ggtgctcatt ctggatgaag taaccagtgc gctcgacggg
     4681 cgcacggaag ccga
//
LOCUS       DBNGVR010000146         3359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_444568, whole genome shotgun sequence.
ACCESSION   DBNGVR010000146 DBNGVR010000000
VERSION     DBNGVR010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_444568"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="AC16A0_03135"
     CDS             <1..319
                     /locus_tag="AC16A0_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIR3104353.1"
                     /translation="TVGLTETEARKDGRAIDVYKTSFKPMKHTLSGRDERMLMKLVVD
                     AETDVVLGCHICGPDAGEMAQLLGIAVRLGAKKSDFDATMAVHPTAAEELVTLREKWQ
                     PGR"
     gene            complement(323..916)
                     /locus_tag="AC16A0_03140"
     CDS             complement(323..916)
                     /locus_tag="AC16A0_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HIR3104354.1"
                     /translation="MSRLQTIPARRGKAVRLAKGQSVKVINTHGNQVVDFWAFNAGDL
                     REYMGMEHCRAFWTRLFPVAGDKMVTNRRRPILIMTEDYSPGRHDTLIAPCDNERYGL
                     LGCTHYHDNCRDNMHAGLAELGYTIPYTPDSLNLFMNIPWRQDGALEWGNCLCKPGDY
                     VTFKAELDSIVVFSACPQDMLPINNGITTEAHFEVID"
     gene            959..1915
                     /gene="speB"
                     /locus_tag="AC16A0_03145"
     CDS             959..1915
                     /gene="speB"
                     /locus_tag="AC16A0_03145"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3104355.1"
                     /translation="MKKDPRFQPVDAAVTPRFAGIATFMRTQRHDVSSDLDIALVGVP
                     FDIGVNYRAGARQGPAAVREASRLTRRVHSTSGIAPYDICNVADVGDAPVNPIDLARS
                     IAMIEAFFAQIHAAGATPLSIGGDHTVPLPILRALAKDQPVGIFHIDSHADTLDTLAD
                     TRINHATTFRRGVEEGLIDPKRTIQIGLRGSRFSPDDIRWGQDAGFTCITFDEYEALG
                     RDAVIRKIQSVLGKGPTYMTIDIDGIDPAWAPGTGVPEIGGLTPRDVQVMIRSLQGMH
                     LVGGDICEVAPCFDPTGITAVTAANLMWEMLCVLADSKARQG"
     gene            1977..>3359
                     /locus_tag="AC16A0_03150"
     CDS             1977..>3359
                     /locus_tag="AC16A0_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3104356.1"
                     /translation="MDDKPQKTDRYRETVFLPRTDFSMKAGLPQKEPELLKRWEELGL
                     YRRLREQSRGRPKYVLHDGPPYANGHLHIGHALNKILKDIVTRSRQMQGFDSNYVPGW
                     DCHGLPIEWKIEEQYREKGKNKDEVPINDFRRECRTFAEHWIKVQREEFKRLGVTGDW
                     DNPYLTMNFEAEALIAKELLKFAASGQLYRGSKPVMWSVVEKTALAEAEIEYQDYQSD
                     TIYVKFPVRFVVRSGNVMSDPLGANVVIWTTTPWTIPGNRAISFSSKISYGLYEVQQA
                     PDGNWAKKGERYILADKLADAVMAAAKVEAFARIADADPSGLVCDHPLAAMGYAFDVP
                     LLDGNHVTDDTGTGFVHTAPGHGADDYNIWMEEVGGPIPFTVNADGYFTKEAPGFEGR
                     RVITDKGEKGDANDAVIRALADAGALVARGRLKHQYPHSWRSKKPIIFRNTPQWFISM
                     SSHGLRDIALR"
BASE COUNT          684 a         1023 c         1037 g          615 t
ORIGIN      
        1 caccgtgggg ctaacggaaa ccgaagcccg caaggatggc agggccatcg acgtctacaa
       61 gacgtcattc aagcccatga agcatacgct ttccggacgt gacgagcgta tgctgatgaa
      121 gctggtcgtg gatgcagaga cggacgtcgt gcttggatgc cacatctgcg gcccggatgc
      181 cggcgaaatg gcccagcttc tcgggattgc cgtgagactc ggggcaaaga agtcagactt
      241 cgacgcaacc atggccgtgc atccgacggc cgccgaagag ctggtcacgt tgcgcgaaaa
      301 atggcagccg ggaagataac gctcagtcga tcacttcgaa atgggcttcg gtggtaatgc
      361 cgttgttgat cggcagcata tcctgtgggc aggccgagaa gacgacgatt gaatccagct
      421 ccgccttgaa tgtcacatag tcgccgggct tgcacaggca attgccccac tcaagcgccc
      481 cgtcctgccg ccacggaatg ttcatgaaca gattgagcga gtccggcgta taagggatgg
      541 tgtatcccag ctctgccagg ccggcgtgca tgttgtcgcg gcaattgtca tgataatggg
      601 tgcagccgag caggccgtag cgctcgttgt cgcaaggcgc gatcagcgtg tcgtgacggc
      661 caggcgaata gtcttctgtc atgatgagga tcgggcggcg gcggtttgtg accatcttgt
      721 ccccagccac ggggaagagc ctggtccaga aagccctgca atgctccatg cccatgtact
      781 cgcgcaggtc gcccgcattg aaggcccaga aatcgaccac ctgattgccg tgcgtgttga
      841 tgacttttac tgactggccc ttcgccagcc gaacggcctt gccacggcgg gccgggatgg
      901 tctgtagtct gctcatcgtc gtctcctgtt tccatttcac attagaggtg ccgtgcagat
      961 gaagaaagac ccacgcttcc agcccgtcga cgcagccgtt accccgcgct tcgcaggaat
     1021 tgccacattc atgcgcacgc agcgccatga tgtctcgtct gacctcgata ttgcgctggt
     1081 cggcgttccc ttcgacatcg gtgtcaatta ccgggccggt gcaagacagg gacccgcggc
     1141 agtccgagaa gcctcgcggc tcacccgccg cgtgcactcg acctcgggca ttgcgcccta
     1201 cgacatatgc aatgtcgcgg atgtgggcga cgcgccggtc aatcccatcg atctggcgcg
     1261 gtcgattgcg atgatcgagg cgttcttcgc gcagattcac gctgcaggtg cgacaccatt
     1321 gtcaatcggc ggcgaccaca ccgtgccctt gcccatcctg cgggccctgg cgaaggacca
     1381 gccggtgggc attttccaca tcgacagcca tgccgatacg ctcgacacgc ttgccgacac
     1441 gcgcatcaat cacgccacga ccttccggcg cggcgtggag gaggggctga tcgatccgaa
     1501 gcggaccatc cagatcggtc tacgcggctc gcggttctcg cctgatgaca tcaggtgggg
     1561 ccaggacgcc ggtttcacct gcatcacctt cgatgagtac gaagccttgg gccgggacgc
     1621 cgtcatcagg aagatccagt ccgttttggg caaggggccg acctatatga ccatcgatat
     1681 cgatgggatc gacccggcct gggcgccggg gacaggtgtt cccgagatcg gcgggctcac
     1741 gccccgggac gttcaggtga tgatccggtc gctgcagggg atgcacctcg tgggtggcga
     1801 catctgcgag gtggcgccgt gtttcgaccc caccggcatc acggcagtga cggcggccaa
     1861 tctgatgtgg gaaatgctct gcgtcctggc tgattccaag gcccgccagg gctgattgcg
     1921 gggcagggca ctggctgcta gaagcgcgtc caagctcagc attggaccgc ttcgccatgg
     1981 acgacaagcc gcaaaagacc gaccgctatc gcgaaaccgt gttcctgccc aggaccgact
     2041 tctcgatgaa ggccggcctg ccgcagaagg aaccggaact tctcaagcga tgggaggagc
     2101 ttggcttgta tcgaaggctt cgggagcaga gccgcggacg accgaagtac gtgctgcatg
     2161 acggcccgcc ctatgccaac ggccaccttc acatcggcca cgcgctgaac aagatcctga
     2221 aggacatcgt cacccgctcg cgccagatgc aggggttcga ttccaactac gttccgggct
     2281 gggactgtca cggcctgccc atcgaatgga agatcgagga gcaatatcgc gagaagggca
     2341 agaacaagga cgaagtcccg atcaacgact tccgcaggga atgccggacc ttcgccgagc
     2401 actggatcaa ggtgcagcgc gaggagttca agcgccttgg cgtgaccggc gactgggaca
     2461 atccctacct cacgatgaac ttcgaagccg aggcgctgat cgcaaaggaa ctcctgaagt
     2521 tcgcggccag cgggcagctc taccgcggct ccaagccggt gatgtggagc gtcgtcgaga
     2581 agacggccct ggccgaggcc gagatcgagt accaggacta tcagagcgac acgatctacg
     2641 tgaagtttcc ggttaggttc gtggtcagat caggcaatgt catgtcggat ccgttgggag
     2701 cgaatgttgt aatctggaca acaactccat ggaccatccc cggcaaccgc gccatcagct
     2761 tctcgtccaa gatctcctat ggactctacg aagttcagca agcgccggac ggcaactggg
     2821 ccaagaaggg cgaacgctac atcctggctg acaagcttgc cgacgcagtg atggcggccg
     2881 caaaagtcga ggccttcgcg cgcatcgcag atgccgatcc aagtggtctg gtctgtgacc
     2941 accctctcgc ggcaatgggc tacgcctttg atgttccgct gctcgatggc aaccatgtga
     3001 cagacgacac cggtaccggc ttcgtgcaca cggcgcccgg ccatggcgcc gacgactaca
     3061 acatctggat ggaagaggtc ggaggcccga ttccctttac cgtcaatgcc gacggctact
     3121 tcaccaagga agcgcccggt ttcgagggtc gccgcgtcat caccgacaag ggtgagaagg
     3181 gggatgccaa cgacgccgtg atccgcgcat tggccgatgc cggggcactc gtcgcacgcg
     3241 gacggctgaa gcaccagtat ccgcattcct ggcgctcgaa gaagccgatc atcttccgca
     3301 atacccccca gtggttcata tccatgtcgt cccatgggct gcgcgacatc gcattgcgc
//
LOCUS       DBNGVR010000147        12027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4963257, whole genome shotgun sequence.
ACCESSION   DBNGVR010000147 DBNGVR010000000
VERSION     DBNGVR010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4963257"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="AC16A0_03155"
     CDS             complement(<1..616)
                     /locus_tag="AC16A0_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3104357.1"
                     /translation="MVKDIGHFIGGKHVKGTSGRFADVYNPNTGEVQARVALASQAEL
                     RAAVENAKAAQPGWAAKNPQVRARVMMKFLDLAAKHAEELATLISVEHGKTIPDALGD
                     LQRGVEVAEFACGIPHLLKGEFTDGAGPGIDMYSMRQPLGVAAGITPFNFPAMIPLWK
                     AAPAIACGNAFILKPSERDPSCPMRLAELFLEAGLPPGILNVVNG"
     gene            complement(735..1685)
                     /gene="speB"
                     /locus_tag="AC16A0_03160"
     CDS             complement(735..1685)
                     /gene="speB"
                     /locus_tag="AC16A0_03160"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3104358.1"
                     /translation="MVDKPDLAFAGAPVMGMGYEPTYSGATSLFRRPYSRDLSGVDMV
                     AWGIPYDLSVTNRGGCRFGPRGIRAASTNLAWFGGPWPWGFDPFETLRMVDYGDCSFD
                     PGFPMEVAASIEAEASAILENGAFLFSMGGDHSVTAPLLRAQAKRHGPIALIQIDAHS
                     DTWAEPVRRIDHGTMFYHAAQDGIIDASHSVQAAIRTLNDETHGFTVLDAAFVHRNGP
                     EATAERICSIVGDRPAYLTFDIDALDPAFAPGTGTPVCGGLSTWQAQEIVRNLKTVNL
                     VGADLVEVSPPFDHAEITALAGASLLLDMICLRAWQLGRR"
     gene            complement(1798..3168)
                     /locus_tag="AC16A0_03165"
     CDS             complement(1798..3168)
                     /locus_tag="AC16A0_03165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3104359.1"
                     /translation="MTTFSELKLSEPILKALAAEGYETPTPIQAKSIPSLLEGRDLLG
                     IAQTGTGKTAAFALPILEHLAKKTERPARGTVRALILAPTRELASQISDSFRTYGKNL
                     RVSRAVVFGGVGIGKQIEILRGGIDVLVATPGRLVDLIERKAVTLSKVEIFVLDEVDQ
                     MLDLGFIHAIRKLTAMLPKQRQNLFFSATMPKEIAGLADALLSNPVRVEVAPVVTTAE
                     RVNQAVIHVDPANKVKLLAELLKDNNMTRTLVFTRTKHGADKVMKHLSAGGFTAAAIH
                     GNKSQANREKALAGFKAGKILVLVATDIAARGIDVDGVSHVVNYDLPFVPESYVHRIG
                     RTARAGATGEAIAFCTPEDRPLLRDIEKTIRQPVPVKEHALGLKNVPALAPTHGWTPM
                     GKGKPGQKPGGNRNRNHGKPQGQKAQGSGKPKPHRKGQSANGGQGAPRRSGGGSWMTQ
                     IGQKQA"
     gene            complement(3390..4052)
                     /locus_tag="AC16A0_03170"
     CDS             complement(3390..4052)
                     /locus_tag="AC16A0_03170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3104360.1"
                     /translation="MSFRHFIFDCDGVLVDSEPLSMRVDVAILAENGIRMSEDEAHAR
                     FVGKTFAAMIAEMEREHGVRFPADASAQKDRRLLELYERELKPVAGISEALAALPRAV
                     HSVASNSPEERVATALRVAGLEAWFGRITTFEHVANGKPAPDVFIESARRAGFRPEDC
                     MVVEDSVTGVTAAARAGCAVLGFTGTHSDPQAHAARLATAGAQKTFTDMRDLPALAKT
                     FL"
     gene            complement(4049..4546)
                     /locus_tag="AC16A0_03175"
     CDS             complement(4049..4546)
                     /locus_tag="AC16A0_03175"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR3104361.1"
                     /translation="MPSHPEITAYRPCVGAMLINREGLVWIGRRFDKQNDEGKGQWWQ
                     MPQGGIDKGEDPGPAALRELAEETAVTSAAIIAESPEWYTYDLPDHLIGKSWKGKYRG
                     QTQKWFALRFLGDDGEINLAPPGHKQEFDQWRWARMDEILDIIVPFKRPVYERVIADF
                     RHLGA"
     gene            complement(4546..5703)
                     /locus_tag="AC16A0_03180"
     CDS             complement(4546..5703)
                     /locus_tag="AC16A0_03180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HIR3104362.1"
                     /translation="MPRDELRQPLRKRSLSERLWAKRPGALATASVVLAVTVAGGAIW
                     LSRIPHPFAGEPVVIAAIPPAEELKTSSTTPDAAAEDTAAADEAAETVLEAEEAPPKD
                     YSQEAAIIMATHRPLKAAPIADVMEQGPDGPLPRVSKQGRKPSDVYAQVTPTAVITSA
                     RPKIAILLGGMGINQRLTQKAIKELPGDISFGFAPYGENLQAQVNRARAKGHEVMLQL
                     PLEPPGYPGINPGPQTLLSDAPEEENLKSLRWMLSRFAGFTGITNYMGGRFLAVPDSL
                     APVMRELKKRGLVYLEDGSVAQSTAPAIAQETGLPMRRASMVIDAKPDAESIRAALDE
                     LVREAEKNGFAIGIGAGLEVTIETVGEFARELQERGVLLVPISATFKGQMG"
     gene            complement(5848..7194)
                     /locus_tag="AC16A0_03185"
     CDS             complement(5848..7194)
                     /locus_tag="AC16A0_03185"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3104363.1"
                     /translation="MTMKNNLRNVGLVAAGAVSGVLAWHAFGVADAASNANTYKQLNL
                     FGDVFDRVRADYVEQPDEAKMVEAAINGMLASLDPHSSFMNAKQFEDMSVQTRGEFGG
                     LGIEVTMENGVIKVVSPIDDTPASRAGIMANDLIIKIDGQDIQGLTLNEAVDKMRGKV
                     GAPITVTVKRGQQDPFDQKLVRDRIRVQSVKYNVENGDIGYIRISSFTEQTQEGLEKA
                     MAAFDKEVGDKVKGYIIDLRNDPGGLLDMAISVSDSFLDRGEIVSTRGRHADETQRFN
                     AQKGDLAKGKPVVVLINGGSASASEIVAGALQDHKRATIIGTRSFGKGSVQTIIPLGG
                     QGALRLTTARYYTPAGRSIQAKGIDADIVIEQELPPELQGKDVSTEGEASLRGHLSNE
                     GGGEEGGGSSAYVPPDKAKDMQLKAAIDLLNGVKVVSNVKPPEKKPADGAETTTAN"
     gene            complement(7191..8447)
                     /locus_tag="AC16A0_03190"
     CDS             complement(7191..8447)
                     /locus_tag="AC16A0_03190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3104364.1"
                     /translation="MKVTASSLRPAMLAACLLLSPLAAAQTAAPDPQQLETVEQQLEG
                     SKAEQDRIAAEVAAAMREQDEIASRLIEISAQIQEQEAAIQASETELQKLAADQVLLM
                     ASLGERQEVLSELLAGLQRLEQNPPPALVVEPEDVLAALRGAMLFGTVVPELQDDAQE
                     LIVELARLDALKTEVAARKQALADDIRRLETSRGEIGGLIAQKKELVARGNADLESER
                     KRAERLAANASNLRQLLAGLAAAREKAEAETRKKAADAAAAARRLEEEKRLPKIAFAQ
                     AKGQLNLPAKGQVLRRFGDPDGLGGKSQGLVIGTRKGAQVTAPADGQVEFAGAFRSYG
                     KVVILNPGSGYRILLAGLEEVTAATGERLRAGEPVGLMGDGPSSVTLLGDVVQNGEPV
                     LYIEFRNSTEAIDSGPWWTANKEARG"
     gene            complement(8502..8984)
                     /gene="rlmH"
                     /locus_tag="AC16A0_03195"
     CDS             complement(8502..8984)
                     /gene="rlmH"
                     /locus_tag="AC16A0_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR3104365.1"
                     /translation="MRLSIIAVGRLKAGPEKELAEEYRKRSEALGRRAGITRLAVTEF
                     AESQAGSAASRIAEEAQLIAGALPPRGFTVVLDEAGKSMTSADFAAFLQRRRDDGTAD
                     LAFVVGGPDGHAQATRAGAGLVLSFGAMTWPHRLVRVLLFEQIYRAVTIMVNHPYHRA
                     "
     gene            complement(8988..9392)
                     /gene="rsfS"
                     /locus_tag="AC16A0_03200"
     CDS             complement(8988..9392)
                     /gene="rsfS"
                     /locus_tag="AC16A0_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3104366.1"
                     /translation="MPKAKPKAGKAAPARKAAKPAKPGLLDMVLAQLDDAKAEAVVTI
                     NLDGKGALADSMVVASGRSNRHVGAIADQLVDRLKEAGHRNLRIEGMPQCDWVLVDAG
                     DVVVHLFRPEVRSFYNLEKLWSEHAPQERVAV"
     gene            complement(9630..10361)
                     /locus_tag="AC16A0_03205"
     CDS             complement(9630..10361)
                     /locus_tag="AC16A0_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3104367.1"
                     /translation="MPESTSYGQFCPVAMAAEIVCSRWTPLLIRELMSGSTRFNDLRR
                     GVPRMSPTLLSKRLKELEESGIVTVDRSAGASDYRLTPAGEELRPIVMALGNWGQRWV
                     EAELTLRTLDPTLLMWDMRRGLRILPPPDLRCTIRFHFPEMKSNQRSYWMVVEPEGSV
                     DLCYVEPGYPIDIYVEGSLRSMTAVWMGLSRLNAEREAGRLEIDGARGLVQSFCGWLA
                     LSPFAREAKRPDANIRPAPHRVIAA"
     gene            10501..11652
                     /locus_tag="AC16A0_03210"
     CDS             10501..11652
                     /locus_tag="AC16A0_03210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3104368.1"
                     /translation="MTALPMTKPGRCPSHLAIAQTLGQQFARHAAKADEEDCFVADNY
                     AVLKTSGLVEAGVPVELGGGGAEIAALADMLRILAHHRSSTALAFSMHTHQVAIPAWR
                     WKVQKVAAVEPLLRRIVAEKLILLSSGGSDWIAGSGRAVRVEGGYSINARKIFTSGAP
                     AGDLLMTGAVLEEDGQEPMVLHFGIPMTSPHVRIDPVWRTLGMRGTGSHDVLIEGHVI
                     RETAVALKRKAGEWHPLFHTIATLAFPLVYAAYLGVAESACSIAVELARKRRADHNVT
                     QLIGRMQTELAGAQMAHRHMVEAASGGTTSAETVNDVMIGKRLVTEHGIRAVEFAMEA
                     AGGAGFYHSQGLERRFRDIQGARYHVLQAGPQATFAGSLALGLPVDKVF"
     gene            complement(11649..>12027)
                     /locus_tag="AC16A0_03215"
     CDS             complement(11649..>12027)
                     /locus_tag="AC16A0_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase family protein"
                     /protein_id="HIR3104369.1"
                     /translation="LGSRTTAETLRGMAPVLKLAHFVWIMGADSFANLHHWHDWLEIP
                     GTLPLAILARPGYSLRALGSPAATRFAEQQVPTPLAPQLVTTPPPAWAFIPMPLRPES
                     STAIRSRQRPAVNAVKSGTGPSD"
BASE COUNT         1958 a         3871 c         4008 g         2190 t
ORIGIN      
        1 cgccgttcac gacattgagg atgcccggcg gaagtccggc ctcgaggaag agttcggcca
       61 ggcgcatcgg gcaggacgga tcgcgctccg agggcttcag gatgaaggcg ttgccgcagg
      121 caatggcggg tgccgccttc cacagcggga tcatggccgg gaagttgaag ggcgtgatgc
      181 cggcggccac gcccagaggc tggcgcatgg agtacatgtc gatgccgggg ccggcgccat
      241 cggtgaactc gcccttcagc aggtgcggga tgccgcaggc gaattccgcc acctcgacgc
      301 cgcgctgaag gtcgcccagc gcatccggga tggtcttgcc gtgttccacc gagatcagcg
      361 tggcaagttc ctcggcgtgc ttggcggcca gatccaggaa cttcatcatc acgcgggcgc
      421 gcacctgcgg gttcttggcc gcccagccgg gctgggccgc cttggcgttc tcgacggcgg
      481 cgcgcagttc cgcctgcgag gccagcgcca cccgggcctg aacctcgccg gtgttggggt
      541 tgtagacatc cgcgaaacgg ccggaagtgc ccttgacgtg cttgccgccg atgaaatgcc
      601 cgatgtcctt gaccatgtaa ccctcgcttg aactcaagat atggcgggca aactaggggg
      661 atggtccccg gcccgcaagt gacagtggcc gcccgataac acgtccagac gggatttgga
      721 atgcgccgcc ggacctagcg ccggccaagc tgccaggccc ggaggcagat catgtcgagc
      781 agcagcgagg ccccggccag cgccgtgatc tcggcatggt cgaagggagg cgagacctcc
      841 accagatcgg cacccaccag attgaccgtc ttcaggttgc gcacgatctc ttgcgcctgc
      901 caggtggaaa ggccgccgca gaccggcgtg ccggtgcccg gtgcaaaggc cgggtcgagc
      961 gcatcgatgt cgaaggtgag ataggccggg cggtcgccga cgatgctgca gatcctttcg
     1021 gcggtggcct cggggccgtt gcggtgaaca aaggcggcat cgagcaccgt gaagccatgg
     1081 gtctcgtcat tcagcgtgcg gatcgccgcc tgtacggagt gggacgcgtc gatgatcccg
     1141 tcctgcgcgg cgtggtagaa catcgttccg tggtcgatgc gccgcaccgg ctctgcccag
     1201 gtgtcggaat gcgcgtcgat ctggatcagc gcgatggggc catggcgttt cgcctgcgcc
     1261 cgaagcagcg gcgcggtgac ggaatggtcg ccccccatgg agaaaaggaa ggccccgttc
     1321 tcgagaattg cggaagcctc ggcctcgatg ctggcggcaa cctccatcgg gaatcccgga
     1381 tcgaaggaac aatcgccgta atcgaccatg cgcagcgtct cgaagggatc gaaaccccag
     1441 ggccagggcc cgccgaacca ggcgagattg gtggacgccg cgcggatgcc gcgcggcccg
     1501 aagcggcagc cgccgcggtt ggtgacgctg aggtcgtaag gaatgcccca ggccaccatg
     1561 tcgacgccgc tcaggtcgcg gctgtagggt ctgcggaaca ggctggtggc gccgctgtag
     1621 gtcggctcat agcccatgcc catgacaggc gcgcccgcaa aggcgagatc cggcttgtcg
     1681 accatgcaaa gttcctcccg aaagcgctca tctagccggc gccgcgccgc cttcacaagg
     1741 gcgcccaacg aaaatggccg ggcaagcccg gccattcctt ggtcgatgca gcaggcgtca
     1801 ggcctgcttc tggccgatct gcgtcatcca gctgccgccg ccggagcggc ggggcgcacc
     1861 ctggccgcca ttggcgctct ggcccttgcg gtggggcttg ggcttgccgc tgccctgggc
     1921 cttctggccc tggggcttgc cgtggttgcg attgcggttg ccgccgggct tctggcccgg
     1981 cttgcccttg cccatcggag tccagccatg ggtcggtgcc agcgccggca cgttcttgag
     2041 gccaagcgca tgctccttca ccggaaccgg ctggcggatg gtcttctcga tgtcgcgcag
     2101 cagagggcga tcttccggcg tgcagaaggc gatggcttcg cccgtggcgc ccgcccgcgc
     2161 ggtgcggccg atgcgatgca cataggattc cggcacgaag ggcaggtcgt agttcaccac
     2221 gtggctgacg ccgtcgacgt cgatgccgcg cgccgcgatg tcggttgcga ccagcaccag
     2281 gatcttgccc gccttgaagc cagccagcgc cttctcgcgg ttggcctgcg acttgttgcc
     2341 gtggatggcc gcggccgtga agcctccggc ggagagatgc ttcatgactt tgtcggcgcc
     2401 gtgcttcgtc cgtgtaaaga cgagcgtgcg cgtcatgttg ttgtccttga gcagctcggc
     2461 cagcagcttc accttgttgg cgggatcgac gtggatcacg gcctggttca cccgttcggc
     2521 cgtggtgacg acgggcgcca cttcgacacg gacgggattg ctgagcagcg cgtcggcaag
     2581 gccagcgatc tccttcggca tggtggccga gaagaacagg ttctggcgct gcttgggcag
     2641 catggcggtg agcttgcgga tggcgtggat gaagccgaga tccagcatct ggtcgacttc
     2701 gtcgaggacg aagatctcga ccttggacag ggtcacggcc ttgcgttcga tgagatcgac
     2761 gaggcggcca ggcgtggcca cgagaacgtc gatgccgccg cgcaggatct cgatctgctt
     2821 gccgatgccg acgccgccga agaccacggc gcgcgagacg cgcaagttct tgccgtaggt
     2881 gcggaaggag tcggagatct gagaggcgag ttcgcgcgtc ggcgccagga tcagcgcacg
     2941 cacggtaccg cgcgccgggc gctcggtctt cttggccagg tgctcgagga tcggcagcgc
     3001 gaaggcggcc gtcttgccgg tgccggtctg ggcaatgcca agcaggtcgc ggccttcgag
     3061 caggctcgga atggacttcg cctggatggg ggtcggggtc tcgtagccct cggcggccag
     3121 cgccttgaga atgggttcgg agagtttcag ttcgctaaaa gtggtcaagt tcgattcttt
     3181 catgtttcgg aacggtgggg aacgcgcgaa ccatgtccga atggccatgc gcggaaatcg
     3241 cgcaaagcct ggagggcgcg taaacagacc cacgtgaaag gcatgttcgg aagatcgtcc
     3301 ggtccgaatg gccacccgag gggcctcacg tcattcttta caagggaccg aagacgatga
     3361 cggctatatg ggcgtgttgc agcgcaatgt caaaggaatg ttttggccag cgccggcaga
     3421 tcgcgcatgt cggtgaatgt tttctgcgcg ccggcggtcg cgaggcgggc ggcatgggcc
     3481 tgcggatcgg aatgggttcc ggtgaagccc agcacggcgc agccggcgcg ggcagccgcg
     3541 gtgacgccgg tgacgctgtc ctcgacgacc atgcagtctt ccggccggaa tccggcgcgc
     3601 cgcgcggatt cgatgaagac gtcgggcgcc ggtttgccgt tggcgacatg ctcgaaagtg
     3661 gtaatgcggc cgaaccaggc ctcgagcccc gccacccgca gcgcggtggc gacgcgctcc
     3721 tccggactgt tcgaggcaac gctgtgaacc gcgcgcggca gggccgcgag ggcttcggag
     3781 atcccggcga ccggcttcag ctcccgctca tagagttcga gcaggcgccg gtccttctgg
     3841 gcgctggcgt cggcaggaaa gcgcacgccg tgctcgcgtt ccatttccgc gatcatggcg
     3901 gcaaaggtct tgcccacgaa gcgcgcatgc gcctcgtctt cgctcatgcg gatgccattc
     3961 tcggcgagga ttgccacgtc gactcgcatc gacagcggtt cgctgtcgac gagcacgccg
     4021 tcgcagtcga agatgaagtg ccggaagctc acgcgccgag atgcctgaag tctgcgatga
     4081 cgcgctcata gaccggccgc ttgaacggca caatgatgtc gagaatctcg tccatgcgcg
     4141 cccagcgcca ctggtcgaat tcctgcttgt gcccgggcgg tgcgagattg atctcgccgt
     4201 catcgcccag gaagcgcagc gcgaaccact tctgcgtctg tccgcggtat ttgcccttcc
     4261 aggacttgcc gatgagatga tcgggcaggt cgtaggtgta ccattccggc gattcggcga
     4321 tgatcgcagc ggaggtgact gcggtttcct cggccagctc gcgcagagcc gcgggcccgg
     4381 gatcctcgcc cttgtcgatg ccgccctgcg gcatctgcca ccactggccc ttgccctcgt
     4441 cgttctgctt gtcgaagcgg cggccgatcc acacgagccc ttcacggttg atcagcatgg
     4501 cgccgacgca gggccggtag gcggtgatct cgggatgcga tggcatcagc ccatctggcc
     4561 cttgaaggtg gcactgatcg ggaccagcag cacgcctctc tcctggagtt cgcgggcaaa
     4621 ctccccgacc gtctcgatgg tgacttccag gcctgcgccg atcccaatgg cgaagccgtt
     4681 cttctcggcc tcgcgcacca gttcgtcgag cgcggcacgg atgctttcgg catcgggttt
     4741 ggcgtcaatc accatgctgg cccgccgcat gggcagtccg gtttcctgtg cgatggccgg
     4801 cgccgtgctc tgggcaaccg agccatcctc gagatagacc aggccgcgct tcttcagttc
     4861 cctcatcacc ggtgccagac tgtcgggtac ggccaggaac cggccgccca tgtagttggt
     4921 gatgccggtg aagccggcaa agcggctgag catccagcgg agcgacttga ggttctcttc
     4981 ctctggcgca tcggacagca gtgtctgcgg accgggattg attcccggat agcccggcgg
     5041 ttcgagcggc agctgaagca tgacctcgtg acccttggcg cgcgccctgt tgacctgcgc
     5101 ctgcaggttc tcgccatagg gcgcaaagcc gaagctgatg tcgcccggaa gttccttgat
     5161 cgccttctgg gtcaggcgct ggttgatgcc catgccgcca agcagaatgg cgatcttggg
     5221 ccgggccgag gtgatgacgg cggtgggcgt gacctgggca tagacatcgg acggcttgcg
     5281 gccctgcttc gaaacgcgcg gcagcggtcc gtccggaccc tgctccatga catcggcaat
     5341 cggcgcggcc ttgagcggcc tgtgcgtcgc catgatgatg gcggcttcct gcgagtaatc
     5401 cttcggcggc gcttcctcgg cctccagaac ggtttcggcg gcctcatcgg ccgcggcggt
     5461 gtcctcggca gccgcgtccg gcgtggtcga agatgtcttc agttcctcgg ccggcgggat
     5521 cgccgcgatc acaaccggct cgccggcgaa gggatgggga atgcgcgaca gccagatggc
     5581 accgccggcc accgtgacgg caagtacgac ggaagcagtg gccagcgcac ccggccgctt
     5641 ggcccagagg cgctcggaca gggatcgctt gcgaaggggt tgccgcaatt cgtcgcgggg
     5701 catgactctc gggtgatctt gtgattcgat tgacccaact gtggagtccc aaccgttaac
     5761 ggcctgttaa ccctgatcgc aagtttccat aatgcagaca ccccgcccgt ttccgggcgg
     5821 ggtgtgtgat gacgcgtggg gttatcgtca gttggcggtg gtggtctcgg cgccatccgc
     5881 cggctttttc tccggcggct tcacgttgga gacgaccttg acgccattga gcaggtcgat
     5941 ggcggctttc agctgcatgt ccttggcctt gtcgggcggg acataggccg aggatccgcc
     6001 gccttcctcg ccgccgccct cattggagag atggccgcgg aggctggctt cgccttcggt
     6061 cgagacgtct ttgccctgca gttcgggcgg cagttcctgc tcgatgacga tgtcggcatc
     6121 gatacccttg gcctggatgg agcggccggc cggcgtgtag tagcgcgccg ttgtcaggcg
     6181 caaggcaccc tgtccgccca ggggaatgat ggtctgtacc gatcccttgc cgaagctgcg
     6241 ggtgccgatg atggttgcac gcttgtggtc ctgcaaggcg ccggccacga tctcggaggc
     6301 cgaggcggaa ccgccattga tcagcacgac aaccggcttg cccttggcaa ggtcgccctt
     6361 ctgggcattg aagcgctgcg tctcgtcggc gtgacgcccg cgggtggata cgatctcgcc
     6421 ccggtcaagg aagctgtccg acaccgagat cgccatgtcg agcaggccgc ccggatcatt
     6481 gcgcagatcg atgatgtagc ctttcacctt gtcgccaacc tccttgtcga aggcagccat
     6541 ggccttctcg aggccttcct gcgtctgctc cgtgaaggag ctgatgcgga tgtatccgat
     6601 atcgccgttc tcgacattgt acttgaccga ctggacgcgg atacggtcgc gcaccagctt
     6661 ctggtcgaag gggtcctgct ggccgcgctt gacggtgacg gtaatgggcg cgcccacctt
     6721 gccgcgcatc ttgtccaccg cttcgttcag cgtcagtccc tggatgtcct gaccgtcgat
     6781 cttgatgatc aggtcattgg ccatgatgcc ggcacgcgag gccggcgtgt cgtcgatcgg
     6841 cgacaccacc ttgatgaccc cgttctccat cgtgacttcg atgcccaacc cgccgaattc
     6901 gccgcgggtc tggacgctca tgtcctcgaa ctgcttggcg ttcatgaaac tggaatgcgg
     6961 atcgagggaa gccagcatgc cgttgatggc ggcttccacc atcttggcct cgtcgggctg
     7021 ttcgacatag tcggcgcgga cgcggtcgaa cacgtcgcca aagagattga gctgcttgta
     7081 ggtattggca ttggaggcgg catcggcgac gccgaaggcg tgccaggcga gaacgccgga
     7141 cacggcaccc gccgcgacca aaccgacatt gcgcaagtta ttcttcatcg tcatccacgc
     7201 gcctctttat ttgctgtcca ccacggacca gaatcgatgg cttcggtgct gttccggaac
     7261 tcaatataca gcacaggttc gccgttttgc actacatcac ccaaaagtgt gaccgaagag
     7321 gggccatccc ccatcaggcc gaccggctcg ccagcccgca gccgttcccc ggtggctgcc
     7381 gtcacttcct caagaccggc caacaggata cgatagccgc tgcccggatt caaaattaca
     7441 actttgccat atgaacggaa ggccccggcg aactccacct ggccgtcggc cggcgccgtg
     7501 acctgggcgc ccttgcgggt gccgatgacc aggccctggg acttgccccc cagcccgtcc
     7561 ggatcgccga agcgccgcaa gacctggccc ttggccggca gattgagctg gcccttggcc
     7621 tgcgcgaagg cgatcttggg aagacgcttc tcctcttcaa gccgccgggc ggcggcagcg
     7681 gcatcggcag ccttcttcct ggtttcggct tctgcctttt cgcgggcggc ggcgaggccg
     7741 gccagcaact gacggaggtt ggaggcattg gcagccagcc tctcggcgcg cttgcgttcg
     7801 ctctcgaggt cggcattgcc gcgcgccacc agttccttct tctgggcgat caacccgcca
     7861 atctcgccgc gcgaggtctc aagccgccgg atgtcgtcgg ccagcgcctg cttgcgcgcc
     7921 gcgacttcgg tcttgagtgc atcaagtcgc gcaagctcga cgatcagttc ctgtgcgtcg
     7981 tcctgcagct cgggcaccac cgtgccgaag agcattgccc cccggagtgc ggccagcacg
     8041 tcctcgggct ccaccaccag ggcgggcggc gggttctgtt ccagccgctg caggccggcc
     8101 agcaactcgg agagcacttc ctggcgctcc cccagactgg ccatcaacag cacctgatcc
     8161 gcggccagct tctgcagctc cgtctcgctg gcctggatgg ccgcttcctg ttcctggatc
     8221 tgggccgata tctcgatcag ccggctggcg atctcgtcct gctcgcgcat ggccgcggcc
     8281 acttcggcgg caatccggtc ctgctccgcc ttcgatccct cgagttgctg ttcgacggtt
     8341 tccagctgtt gcggatcggg ggcggcagtc tgggcggcgg ccagcggcga cagaagaaga
     8401 caggcggcca gcattgccgg acgcagactt gaggcggtga ccttcacgct cgggaaactg
     8461 ggtatcgcga ttgtccaaaa tatggccgga gcggctgcga ctcaggcccg gtgataggga
     8521 tgattcacca taatcgtcac tgcccggtaa atctgctcga aaagcagcac gcgcaccagg
     8581 cggtgcggcc aggtcatggc accgaaggac aggacgaggc cggcacccgc gcgcgtagcc
     8641 tgggcatgcc cgtccgggcc gccgacgacg aaggcgagat cggcggttcc gtcatcgcgc
     8701 cgtctttgca ggaaggcggc gaaatcggcg ctggtcatgg acttgccggc ctcgtccagc
     8761 acgacggtga agccgcgcgg cggcaaggcg ccggcgatca gctgcgcttc ctcggcgatg
     8821 cgcgaagctg cgctgccggc ctgggattcg gcaaactcgg tcacggcaag gcgggtgata
     8881 ccggctctcc gcccaagggc ttcgctgcgc ttgcggtact cctcggcaag ctccttttcc
     8941 ggaccggcct tgagacggcc cacggcaatg atcgagagcc gcacggctca gaccgcgacg
     9001 cgttcctgcg gtgcgtgttc cgaccacagt ttctcgaggt tgtagaagct gcgcacctcg
     9061 ggacggaaca gatgcacgac cacatccccg gcatcgacca ggacccagtc gcactgcggc
     9121 atgccttcga tccggagatt gcggtgaccg gcctccttca gcctgtccac gagctggtcg
     9181 gcgatggcgc cgacgtgccg gtttgaacgg cccgaggcca cgaccatgct gtcggccagc
     9241 gcgcccttgc cgtcgaggtt gatggtcacc acggcctcgg ccttggcgtc atcgagctgc
     9301 gccagcacca tgtccagcag accgggcttg gccggctttg cggctttgcg tgcaggggcg
     9361 gcctttccgg ccttgggctt ggccttcggc accttggcct tggcagcttt cgcgggcttc
     9421 gcggtcttga ccggcttggc tgccgccttt ttggcaacag gcttcgcctt cgctgcggcc
     9481 ttaggcgcag cgttcttctt cgctggtgct ttcaggggta tcgtcctctt tcaatgcgca
     9541 ggtcttcatc agactggcga tcatgtcccg aacaggtgac aacaacggca aactaggccc
     9601 tccggtcctg ccattcaagg cccagtatgt tacgctgcga tgacacggtg aggcgccggc
     9661 cgaatgttgg catccggccg cttggcttcg cgggcaaagg ggctaagtgc cagccagccg
     9721 caaaaggact gcaccaggcc acgggcgccg tcgatctcga gccgaccggc ctcgcgttcg
     9781 gcattcagtc ggctcagccc catccacacg gcagtcatgc tgcgcagcga tccctcgaca
     9841 tagatgtcga tcggatagcc aggttccaca tagcagagat ccacgctgcc ttcaggttcg
     9901 accaccatcc agtaggatcg ctggttcgac ttcatttccg gaaagtggaa tctgatcgtg
     9961 cagcggaggt cgggcggcgg caagatgcgc agtccgcgcc gcatgtccca catcagcagc
    10021 gtcggatcga gtgtgcgcag cgtcagctcg gcttccaccc agcgctgtcc ccagtttccc
    10081 agtgccatca cgatcggccg gagttcttcg ccggccggag tcaaacggta gtcgctcgcg
    10141 ccagcactcc ggtcaaccgt gacgatcccg gattcctcca gctccttcag gcgcttcgac
    10201 agcagcgtcg gagacatgcg tggaacgccg cggcgcaggt cgttgaaccg ggtcgatccg
    10261 gacatgagct cgcggatcag cagtggagtc cagcgtgaac agacgatctc cgccgccatc
    10321 gcgaccgggc agaactgccc gtagcttgtg gattcgggca tgcgtacctc cctgatgatt
    10381 aagccagatc ggcagcacca taccatgcct gtcagccccc gaggcagtac agattctgca
    10441 ctggagcgac ggaagcggac gggccaacaa gctgctcact gctgatcgga ggacatgctc
    10501 atgactgctc tgcccatgac gaagccggga cgctgcccgt cgcatcttgc cattgcccag
    10561 acgctgggcc agcagtttgc gcggcacgcc gcaaaggccg acgaggagga ctgcttcgtc
    10621 gccgacaact atgccgtgct gaagacgtca ggccttgtgg aggcgggcgt tcccgtggag
    10681 ttgggcggcg gcggcgccga aatcgcagca ctcgccgaca tgctgcggat acttgcgcat
    10741 caccgcagtt cgaccgcact cgccttctcg atgcacacgc atcaggtggc gatcccggca
    10801 tggcgctgga aagtgcagaa agtggctgcc gtcgagcccc tgctcaggcg catcgtcgcc
    10861 gagaagctga tcctgctctc gagcggcggc tccgactgga tcgcaggctc cggacgggcc
    10921 gtgcgcgtcg agggcggcta cagcatcaat gcccgcaaga tcttcacctc aggcgcgccg
    10981 gccggcgatc tcctgatgac cggcgcagtg ctcgaagagg acggacagga accgatggtc
    11041 cttcacttcg gtataccgat gacttcgccc catgtgcgca tcgatccggt ctggcgcacg
    11101 ctgggcatgc gcggcacagg ctcgcatgac gtgctgatcg agggccatgt gattcgggaa
    11161 acggccgtcg ccctcaagcg caaggccggc gaatggcatc cgctgttcca taccatcgcg
    11221 acgctggctt tcccgctggt ctacgccgcc tatcttggcg ttgccgaaag cgcctgcagc
    11281 atcgccgtcg aacttgcccg caagcgccgc gccgaccaca atgtcacgca gctcattgga
    11341 cgcatgcaga cggaacttgc gggcgcacag atggcgcatc gccacatggt cgaggcggcg
    11401 tcgggcggca ccacgtcggc cgagaccgtc aacgacgtga tgatcggcaa gcgcctcgtg
    11461 accgagcatg gcattcgtgc cgtggagttc gcgatggagg ccgccggcgg tgcgggcttc
    11521 taccactcac agggtctcga gagaaggttc cgcgacatcc agggcgcgcg ctaccacgtc
    11581 ctgcaggctg gtccgcaagc gaccttcgcc ggctcgctcg cgctcgggct ccctgtcgac
    11641 aaggttttct agtcggaggg tcccgtaccg gacttgaccg cgttcacagc aggcctctgt
    11701 ctgctgcgaa ttgcggtcga gctctccggc cggagcggca tggggatgaa ggcccaggcg
    11761 ggcggcggcg tcgtgaccag ctggggcgca aggggcgtgg gaacctgctg ctcggcaaag
    11821 cgcgtggccg cggggcttcc gagcgcgcgt aacgaatatc cgggccgcgc cagaatggcg
    11881 agcgggagtg ttccgggaat ctccagccag tcgtgccaat ggtgcaggtt ggcgaagctg
    11941 tcggcgccca tgatccacac gaaatgtgca agcttcagca ccggcgccat cccgcgcagg
    12001 gtctccgcag tggtgcgcga tcccagc
//
LOCUS       DBNGVR010000148         6926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5158839, whole genome shotgun sequence.
ACCESSION   DBNGVR010000148 DBNGVR010000000
VERSION     DBNGVR010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5158839"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC16A0_03220"
     CDS             complement(<1..447)
                     /locus_tag="AC16A0_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3104370.1"
                     /translation="MINTNELAKQVSASGRRAGGRESRRAMRAAPIASFPTLVRNIPV
                     YQMVPDEAVELIHEESLKILEEVGCEFRDDEAIVMWKAAGADVKGTRVHIDRALLMSL
                     VSQVPSEFTLNARNPERTLKVGGRNTVFVPMYGAPYVRDLDNTRRYG"
     gene            complement(536..1438)
                     /locus_tag="AC16A0_03225"
     CDS             complement(536..1438)
                     /locus_tag="AC16A0_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3104371.1"
                     /translation="MVRRHYSLPSLNALAAFEAAARHLSLTRAATELTVTPGAVSKQV
                     KSLESEIGRPLFLRLHRAMELTPEGETVARALQEAFERVSATLQQVSHNPALRSVSIG
                     STMAFAQFWLMPRLHDFWNAHQDIVIDHMISDRNADLFRNDVDLRIRYGDGGWPDETA
                     VKLFDDRIAAVASPGFAARHKVKVKQAKDMAGLPLLSVEGVDWTWTTWSDFLRATGAP
                     HRKLGLRRFNSYVIALQAAQDGQGLALGWMNMIKPLLSKRALVQVTSNEIAAPQAFYV
                     TWNSKRALSREAETLRDWLLSQSA"
     gene            complement(1560..2648)
                     /gene="dctP"
                     /locus_tag="AC16A0_03230"
     CDS             complement(1560..2648)
                     /gene="dctP"
                     /locus_tag="AC16A0_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR3104372.1"
                     /translation="MTIKKKTDLAADRILASRRSMLRNIGVGTAAVAASSLAAPAIVR
                     AQAPIKWRLQTYSGAPLGAHVIKPQIDAFNAAANGEMEIELYYADQLVPTAELFRALQ
                     NGTLDAVQSDEATMASPVDISVFGGYFPFATRYSLDVPALFRYYGLKEIWEEAYSEVE
                     NVTWLSCGAWDPLHIFTKEPIKSLADMNGKRVFGVPTAGKFLAKYGLVPVTVPWDNVE
                     VAIQTGELDGVAWCGFTEAYEVGWADVCNYALTNSVTGAWFGSYFANTASWNKIPAHL
                     KELFLITIDQSHYYRAVWYWGGEAKLRVEGNKMELTSLPDNEWAQVVTDSQAFWDEVA
                     ASSPRAGKVVQAFKKYAETMEKAGYPYR"
     gene            complement(2714..4036)
                     /locus_tag="AC16A0_03235"
     CDS             complement(2714..4036)
                     /locus_tag="AC16A0_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3104373.1"
                     /translation="MSYELIALSMFGMMMVMLLTGQRVFAAIGFVASAAALLLYGEGA
                     VEMPFSQVFKLFNWFPMLTLPLFIYMGYILSESGIAEDLYRMLHVWFGRVRGGLAIGT
                     ILLMVVISAMNGLSVAGMAIGATIALPEMLRRGYDKVMITGVVQGGSSLGILIPPSVV
                     LVLYGMIARQPVGQLWLAGVIPGLLMAAIFVLYIVIRCKLNPSLAPTVKDEELNMPFA
                     EKLRLLRAGIIPFLIFFFMTGLFVFGYTSLVESSAVGATAATLAAAAKRRLNWPLLVK
                     TSRQTLEVSAMFLWLILAALAFGAVFDGLGAARALENLFIGELQMEPWMILTMMMATF
                     IVLGVFLDDTAMLVIVAPLYIPLVKILGYDLVWFGVLYTITCQIAYITPPFGYNLFLM
                     RALAPKEVTLVDIYRSIWPFVIMMVGVIVLVGIFPQLALWLPQYIYAK"
     gene            complement(4033..4536)
                     /locus_tag="AC16A0_03240"
     CDS             complement(4033..4536)
                     /locus_tag="AC16A0_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR3104374.1"
                     /translation="MIRSYVKWIDAISEKVGIAAMALIALMIGILLLDAVTRNVIRMP
                     LHWCIELAQFTLAAYYFMGGPMTLKDESHVRMDLIYGHLSSRGKHWVDLITMFCMLFY
                     LGVMLIGGISSLRYAIETNETRFSIWNPSMIPIKALMVACLVLMVLQAIALAIRHACA
                     LRGKPIL"
     gene            4861..6216
                     /locus_tag="AC16A0_03245"
     CDS             4861..6216
                     /locus_tag="AC16A0_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3104375.1"
                     /translation="MRTQFDIAVIGGGVVGLAILRRFAMSGLRCVLLERGADILSGAS
                     KGNSALLHTGFDAPAGSLELACMQAGYEEYLAIRDRLNLPLLESAALVVAWTEKELAK
                     LPEIVTRAHRNGVRDVEEIGRDDLRRREPHLSEAALGAVRVPREHIIDPWSAPLAYAH
                     QALAHGAEIRRKAEVTSGTYDGERWQLATAAGNLRARVVVNAAGNFGDLVEAMARPSP
                     FTITPRRGQFVVFDKPAARLATAIILPVPTERTKGVVLFRTIFGNLAIGPTAEDVDER
                     DVAACDQPTLERLRARAIEMVPELSGIGVTAIYAGLRPATERKDYIIEALQTQNWLTV
                     GGIRSTGLTAALGIARHVATLYEETFDSLSKAPDPIWTPVPNLAEHLPRPYMTGGEVV
                     CHCELVTRQEIQAALSGALPAGDLIGLKRRTRDTMGRCQGFYCSANVAALYREAADAG
                     S"
     gene            6203..>6926
                     /locus_tag="AC16A0_03250"
     CDS             6203..>6926
                     /locus_tag="AC16A0_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3104376.1"
                     /translation="MQDRDVVIIGGGPAGLSAARRLAELGIRNVTLLEREAEAGGVPR
                     HCGHLGFGWQSHRRIWSGPHFAAAMRGSVEGLDIRTGTTVLQIGKDGVLRIRDRSGLG
                     EIAARRILIATGTRETPRAPRFAGGSGPQGVLTTGALQQHVYLQGFRPFKAPVIVGTE
                     WVSFSAILTCRHLGIRPVAMIEAQARTSAPWPGGPIARLIYGVPVLLNSRILEIRGKQ
                     QVEAVAIKSDGQRRELACDGVIF"
BASE COUNT         1319 a         2203 c         2229 g         1175 t
ORIGIN      
        1 accatagcgc cgggtgttgt cgagatcgcg cacatagggg gcgccataca tgggcacgaa
       61 taccgtgttg cgcccgccca ccttcaacgt gcgttccgga ttgcgcgcat tgagcgtgaa
      121 ttcggacggc acctgcgaca ccaggctcat caacagggcg cggtcgatgt ggacgcgcgt
      181 gcccttcaca tcggcgccgg ccgccttcca catgacgatg gcctcgtcgt cgcggaattc
      241 gcagcccact tcttcgagga tcttcaagga ttcctcgtgg atcagctcga ccgcctcgtc
      301 cggcaccatc tggtagacgg gaatgttgcg caccagcgtg gggaacgatg caatgggtgc
      361 ggcccgcatg gcgcggcggc tttcgcgccc acccgccctg cggcccgatg ccgagacctg
      421 cttcgccagt tcattggtgt tgatcatgcc gggcctcgtg atttgcatgc cgattaagcg
      481 cacggtacct gcaacgcgca acccgaagaa gtcaccagcc tcgcttgagg gaaactcaag
      541 cggactggga gagcagccag tcgcgcaggg tctcggcttc gcgcgacagc gcccgcttgc
      601 tgttccatgt cacatagaag gcctgaggtg ccgcaatctc gttgctcgtg acctgcacca
      661 gcgcgcgctt cgacagcagg ggcttgatca tgttcatcca gccaagggcc aggccctgcc
      721 cgtcctgggc cgcctgcaat gcgatgacgt agctgttgaa gcggcgcagg ccgagcttgc
      781 ggtgcggtgc cccggtggcg cgcaggaagt cggaccaggt ggtccaggtc cagtccacgc
      841 cctcgacaga gagcaggggc aggcccgcca tatccttcgc ctgcttcacc ttgaccttgt
      901 ggcgcgcggc gaaacccggg ctggccacgg cagcgatccg gtcgtcgaag agcttcacgg
      961 cggtctcgtc cggccacccg ccatcgccat aacggatgcg caggtccacg tcgttgcgga
     1021 acagatccgc gttgcggtcg gagatcatgt ggtcgatgac gatgtcctga tgcgcattcc
     1081 agaagtcatg cagccgcggc atcagccaga actgggcaaa ggccatggtc gagccgatgg
     1141 agacgctgcg cagcgcagga ttgtggctga cctgctgcag cgtcgcggag acgcgctcga
     1201 aggcttcctg cagagcccgg gccacggttt cgccttccgg cgtcagttcc atggcccggt
     1261 gaaggcgcag gaacagcggc ctgccgatct ccgactcgag cgacttgacc tgcttcgaca
     1321 cggcgcctgg cgtgacggtg agctcggtgg ccgcgcgggt aagactcaga tgccgcgccg
     1381 ccgcctcgaa ggcggcgagg gcattgagcg acggcaggct gtagtggcgg cggaccataa
     1441 agcagtgaac cagcacaggg ctttcggatg agctattctc aacctacgat cccgcttgcg
     1501 caagaaaaat gcgcggccca ggagccgcgc attctgcttt cgaatcgatc cggctggcct
     1561 cagcgatagg ggtagccagc cttctccatg gtttcggcat acttcttgaa ggcctgcaca
     1621 acctttccgg ctcgcgggct ggaggcggca acctcgtccc agaaggcctg ggagtcggtc
     1681 acgacctgcg cccattcatt gtcgggcagc gaggtcagtt ccatcttgtt gccctcgacg
     1741 cggagcttgg cctcgccgcc ccagtaccag acggcacggt aatagtggga ctggtcgatg
     1801 gtgatgagga agagttcctt cagatgcgcg gggatcttgt tccacgacgc cgtattggcg
     1861 aaatagctgc cgaaccaggc acccgtgacg ctgttggtca gcgcgtagtt gcagacgtcg
     1921 gcccagccca cttcataggc ctccgtgaat ccgcaccagg ccacgccatc gagttcgccg
     1981 gtctgaatgg cgacttcgac gttgtcccac ggcacggtca ccggaaccag gccgtacttg
     2041 gccaggaact tgcccgccgt cggcacgccg aagacgcgct tgccgttcat gtcggcgagg
     2101 ctcttgatcg gctccttggt gaagatgtga agcggatccc aggcgccgca ggaaagccag
     2161 gtcacgttct cgacctcgct ataggcttcc tcccagattt ccttcaggcc gtagtagcgg
     2221 aacagcgccg gcacatcgag gctgtagcgc gtggcgaagg ggaaatagcc gccgaatacc
     2281 gagatgtcga cgggcgaagc catggtggct tcgtccgact gcacggcatc aagcgtcccg
     2341 ttctgcagtg cacggaacag ttccgccgtc ggcacgagct ggtcggcata atagagctcg
     2401 atctccatct cgccattggc agccgcgttg aaggcgtcga tctgcggctt gatcacatgc
     2461 gcaccgagcg gggcacccga ataggtctgc agccgccact tgatcggagc ctgggcccgc
     2521 acgatggcag gcgcagccag cgaggaggcc gcaaccgctg cggttccaac gccaatgttc
     2581 ctgagcatcg agcggcggct cgcgaggatc ctgtccgctg cgagatcggt cttcttcttg
     2641 atcgtcatgt cattactctc cctcttgctt gttttggtcg ttgcggcaaa aaactcgttc
     2701 cgttccggcc tcttcacttt gcgtaaatgt actgcggcag ccacagcgcc agctgcggaa
     2761 aaatcccgac cagcacaatg acaccgacca tcatgatgac gaagggccag atggacctgt
     2821 agatgtccac gagtgtgacc tccttcggcg cgagcgcccg catcaggaag agattatagc
     2881 cgaagggcgg cgtgatatag gcgatctggc aggtaatggt gtagagcacg ccgaaccaga
     2941 ccagatcgta tccgagtatc ttgaccagcg gaatgtagag cggcgccacg atcaccagca
     3001 tggcggtatc atcgaggaat acgccaagca cgatgaaggt tgccatcatc atcgtgagga
     3061 tcatccaggg ctccatctgg agctcgccga tgaagagatt ttccagcgcg cgcgccgcac
     3121 cgagcccgtc gaacacggcg ccgaaggcca gggctgcaag gatcagccag aggaacatgg
     3181 cggacacctc gagcgtctgc cgcgaggtct tgaccagcaa gggccagttg agccgccgct
     3241 tggccgccgc cgcaagggtt gctgcggtgg caccgacagc cgagctttcg accaggctgg
     3301 tgtatccgaa aacgaacagc ccggtcatga agaagaagat caggaacggg atgatgccgg
     3361 cgcgcagcag cctgagcttc tccgcaaagg gcatgttcag ctcttcgtcc ttcaccgtcg
     3421 gcgccagcga agggttcagc ttgcagcgga tgacgatgta gaggacgaag atcgcggcca
     3481 tgagaaggcc ggggatgacc cccgccagcc agagctggcc gaccggctgg cgcgcgatca
     3541 tgccataaag cacgaggacg acgctgggcg gaatcaggat gccgagcgag gaaccgccct
     3601 gcaccacccc ggtgatcatg accttgtcgt aaccgcgtcg cagcatttcg ggcagcgcga
     3661 tggttgcacc gatcgccatg ccggcgaccg acaggccgtt catcgccgag atcaccacca
     3721 tgagcaggat cgtgccgatg gcaagaccgc cgcggacccg gccgaaccag acgtgcagca
     3781 tgcggtagag gtcttccgcg atgccggact cggagaggat gtagcccatg tagatgaaca
     3841 ggggcagcgt cagcatcggg aaccagttga acagcttgaa cacctggctg aagggcatct
     3901 cgacggcacc ttcgccatag agcagcaggg ccgcggcaga ggcgacgaaa ccgatggccg
     3961 cgaagacgcg ctgtccggtc agcagcatga ccatcatcat gccgaacatg gagagcgcga
     4021 tgagttcgta gctcatagga tgggcttccc tctcagcgca caggcatgcc tgatggcaag
     4081 cgcaattgcc tgcaggacca tgagcacaag gcaggcaacc atcaatgcct tgatgggaat
     4141 catcgaggga ttccagatcg agaaccgcgt ctcgttggtc tcgatggcat atctcaggct
     4201 ggagatgccg ccgatcagca tcacgcccag atagaacagc atgcagaaca tggtgatgag
     4261 atcgacccag tgcttgccgc gcgacgagag atgaccgtag atgaggtcca ttcgcacatg
     4321 cgactcatcc ttgagcgtca tggggccgcc catgaagtag taggcggcca gcgtgaactg
     4381 cgcgagttcg atgcaccagt gcagcggcat gcggatgaca ttgcgggtca cggcatcgag
     4441 cagcaggatt ccgatcatca aggcgatcag cgccatggcg gcaatgccga ccttctccga
     4501 gatcgcgtca atccacttga cgtaggacct gatcacgccg cacctctgct gccagagcgg
     4561 tagaatccgt gcggcctgcc atttgccggc gccctgcgag gctgcccgaa acgcccggca
     4621 gcgacagtgc gcggcgcgga cacatgcgtc acgggccccg ggatcaatcc ggcggcctgc
     4681 ccggatgcag caggcatttg cactccccct ctcgtcttct tattctgaat aaaactttac
     4741 agcggccatg gaagcttggc aactgagttt cagaatcggg aaacgcgccg gacgcacaga
     4801 ccgttgtcga tacggcaagg cttgcaggaa cgaactgacg gggagaaaca tccggacaag
     4861 atgcggactc aattcgatat cgccgtgatc ggcggcgggg tggtgggact tgcgatcctg
     4921 cgccggttcg ccatgtccgg actgcgctgc gtgctgttgg agcgcggcgc cgacatcctg
     4981 tcgggcgcct cgaagggcaa cagcgccctg ctgcataccg gcttcgacgc gccagccggc
     5041 agcctcgaac tcgcctgcat gcaggcggga tacgaggaat acctggccat cagggaccgg
     5101 ctgaacctgc ccttgctcga aagcgcagcc cttgtcgtgg cgtggacaga aaaagaactc
     5161 gcgaagctgc cggagatcgt gaccagggcg caccggaatg gggttcgcga tgtcgaggag
     5221 atcggccgcg atgacctgcg caggcgggaa ccgcacctgt cagaagccgc gctcggtgcc
     5281 gtacgggtgc cgcgcgagca catcatcgat ccctggtcgg ccccgctcgc ctatgcgcac
     5341 caggccctgg cccatggtgc ggaaatccgg cgaaaggcgg aggtcacgtc gggcacatat
     5401 gacggcgagc gctggcaact cgccaccgcc gccgggaacc tgcgggcgcg cgtcgtcgtc
     5461 aatgcggcag ggaacttcgg cgatctcgtc gaggccatgg cccggccctc gcccttcacc
     5521 atcacgccgc ggcgcggaca gttcgtggtg ttcgacaagc cggccgctcg gctcgccacg
     5581 gccatcattc tcccggtgcc aaccgagcgc accaagggcg tggtgctgtt ccgcaccatc
     5641 ttcggcaatc ttgcgatcgg tcccacggcc gaggatgtcg atgaacgcga cgtcgcggcc
     5701 tgcgatcagc cgacactcga acggctcagg gcgcgcgcca tcgagatggt cccggaactc
     5761 tccggcattg gcgtgaccgc catctatgcc gggcttcgcc ccgccactga gcgcaaggat
     5821 tacatcatcg aggcgctgca gacgcagaac tggctcacgg tgggcggcat ccgctccacc
     5881 gggctgacgg cagcgctggg catcgcccgt catgtcgcaa cactctatga ggaaaccttc
     5941 gactcactgt ccaaggcgcc agacccgatc tggacaccgg tgccaaatct cgcggaacat
     6001 ctgccccgcc cctacatgac cggcggcgag gtggtctgcc actgcgagct cgtgacgcgg
     6061 caggaaattc aggcggccct gtcaggcgcc ttgcccgccg gcgacttgat cggcctgaag
     6121 cgccgcaccc gcgacaccat gggccggtgc cagggcttct actgttcggc caatgtagcc
     6181 gccttgtacc gggaggccgc cgatgcagga tcgtgatgtc gtcatcatcg gcggcggacc
     6241 ggcgggactg tcagcggccc gccggcttgc agaactcggc atccggaatg tcacgctgct
     6301 ggaacgcgaa gccgaagccg gcggcgttcc gcgccactgc ggccacctcg gcttcggctg
     6361 gcagagccat cgccgcatct ggagcggccc gcatttcgcg gccgccatgc gcggcagcgt
     6421 cgaaggcctc gacatccgca ccggcaccac cgtgctgcag atcggcaagg acggtgtcct
     6481 gcgcatccgc gatcgcagcg gactgggcga aatcgccgcc cggcgcatcc tgatcgcgac
     6541 cggaacccgc gagacgccgc gggctccgcg tttcgccggc ggctcgggac cgcagggcgt
     6601 gttgaccacg ggcgccctgc agcagcatgt ctatctgcag ggcttcaggc ccttcaaggc
     6661 gccggtgatc gtggggaccg aatgggtgtc cttctcggcg atcctcacct gccgccatct
     6721 cggcatcagg ccggtggcaa tgatcgaggc gcaggcacga acctctgcgc catggcccgg
     6781 cggtcccatt gcacggctga tctatggcgt gccggtgctg ctgaacagcc ggattctcga
     6841 gatcaggggc aagcagcagg tcgaggccgt cgcgatcaag tcggatgggc agcgccgcga
     6901 gctggcctgc gacggcgtga tcttca
//
LOCUS       DBNGVR010000149         3600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5159205, whole genome shotgun sequence.
ACCESSION   DBNGVR010000149 DBNGVR010000000
VERSION     DBNGVR010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5159205"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16A0_03255"
     CDS             complement(<1..219)
                     /locus_tag="AC16A0_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104377.1"
                     /translation="MTRIRFLGLAVSAAATVFGTGAAAAGPSPVFDHITLTPTGIPPV
                     IIVESQDGKKWSKIRPQKLLLSGKVDVAM"
     gene            complement(329..1222)
                     /locus_tag="AC16A0_03260"
     CDS             complement(329..1222)
                     /locus_tag="AC16A0_03260"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR3104378.1"
                     /translation="MNAPFPSSQTAALMSLDPLHAFLQNLSIEITGKQIEKLPLLRQR
                     LVPGTKVFIALIDPADVAVQLESARQLKDAGFQAIPHVPARFVRDAEDLKSRIAALAG
                     AGVTEMLVLGGGAPQPIGIYDAALQLLETGVFQANGITRIGLAGHPEGNPDITKIHGE
                     AVLLKALKEKQAYLKANGIDGFIATQFLFEAAPVAEWARTLRAEGVGLPIHVGVPGPA
                     TIKTLVKYATMCGVGNSARFLRKQAMNITKLLTVNTPDDFVQDLAELHASRPELGIAG
                     PHLYPFGGFDKLFDWLASKQG"
     gene            1418..2407
                     /locus_tag="AC16A0_03265"
     CDS             1418..2407
                     /locus_tag="AC16A0_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HIR3104379.1"
                     /translation="MFGDKPMQLPHHFALVMVPEFTMMPVTSAIEPLRIANRLSEKSL
                     YRWTMHSVDGKPVAASNGILAMVDGDLAVVPENATVIICGGLNVQHHADKRLVAWLRK
                     IARKGPDIGAVCTGAHILAEAGLLDGYRCTIHWENLPGFSEAFPEIDATGGLFEIDRD
                     RFTSAGGTTALDMMLTLIASQHGPELASSVAEGILHAPIRHHSEHQRMSLPARIGARH
                     PKLVGIIEEMEGNLEEPLSPSLLARQAGLSTRQLERLFRRYLDRSPKRYYLELRLKKA
                     RSLLLQTDMSVINVALACGFSSPSHFSKCYRAFYGRTPYRERGIPVIEAAEHA"
     gene            complement(2611..>3600)
                     /locus_tag="AC16A0_03270"
     CDS             complement(2611..>3600)
                     /locus_tag="AC16A0_03270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3104380.1"
                     /translation="NGTQLRVIQQNFKLGDNERNYSFLVAGNFDELKGEIDAFRRTLF
                     VVLGLLGIGLMVAVLLQVNYGLLPLKRLRAGVTAIREGRAEKLEGEFPAEIRPVADEL
                     NLLIKSNADIIERARTQVGNLAHALKTPLSVLTNEAHVHKSPFASKVVEQTNVMRDHV
                     SLYLDRARRAARAQGLGAVTEIKPVLEGIARTTERIHRDKGVTVKVKCKGAYKFRGEK
                     QDFEEVMGNLIDNACKWAAKRVVVKASLGGAAAHQGRTWLTLLVDDDGPGLPAERRAD
                     ALKRGQRLDETKPGSGLGLSIVKETAQMYGGSIELDDAELGGLRVRLILPAAA"
BASE COUNT          639 a         1177 c         1125 g          659 t
ORIGIN      
        1 catcgcaaca tccaccttgc ccgacagcag cagcttctgc ggccggatct tgctccactt
       61 ctttccatcc tgggattcga caatgatcac gggcggaatg ccggtcggcg tcagcgtgat
      121 atgatcgaac accggcgagg gaccggcggc ggcggcaccg gtgccgaaga ccgtggctgc
      181 ggctgaaacg gcgaggccga ggaagcgaat gcgggtcatg gcagaactcc ttctttgctg
      241 atgtccaaac gatagacaca aggctagtgg agtcagtctg aatggaatcg ggacggcctg
      301 ttcacacgcg gttcaccgga ggaaccggtc agccctgctt gctggccagc cagtcgaaga
      361 gcttgtcgaa gccgccgaag ggatagagat gggggccggc gatgcccagc tcgggacggc
      421 ttgcgtggag ctccgccagg tcctgcacga agtcgtcggg cgtattgacg gtgagcagct
      481 tggtgatgtt catcgcctgc ttgcggagga agcgcgccga attgcccacg ccgcacatgg
      541 tggcatattt caccagcgtc ttgatggtgg cggggcccgg cacgccgaca tggatgggaa
      601 ggccgacgcc ctcggcccgg agcgtccggg cccattcggc caccggcgcg gcctcgaaga
      661 ggaactgcgt ggcgatgaag ccgtcgatgc cattggcctt gaggtaggcc tgcttctcct
      721 tcagcgcctt gagcagcacc gcctcgccat ggatcttggt gatgtcggga ttgccttcgg
      781 gatgtccggc gaggccgatg cgggtgatgc cattggcctg gaacaccccg gtctcaagca
      841 gctggagggc agcatcgtag atgccgatcg gctgcggcgc gccgccgccc agcaccagca
      901 tctcggtcac gccggcgccg gccagggccg cgatgcgcga cttgagatcc tcggcgtcgc
      961 gcacgaagcg ggccggaacg tgcgggatgg cctggaagcc cgcgtccttc agctgacgcg
     1021 ccgattcgag ctggaccgcc acgtcggccg ggtcgatcag ggcgatgaag accttggtgc
     1081 ccggcaccag ccgctgtctc agcaggggca gcttctcgat ctgcttgccg gtgatctcga
     1141 tggacaggtt ctgaaggaag gcgtggaggg gatcgaggct catcaaggcg gctgtctggc
     1201 tggaggggaa gggagcgttc atggtcagtg gggtccggtt cagacccgga cattgcaatg
     1261 cgaagccttc cggcgcaccg gattttgcga cagggcggtt acgaatgcga cttatgggag
     1321 gcctctcacc ctcatgtcgt ttctgtcatg tctcatgacg cggatggaca aatgaccgat
     1381 tttggacgag aaaggcggca acaggagttc ctcgcccatg ttcggcgaca agcccatgca
     1441 attgcctcac catttcgccc tcgtcatggt gccggagttt accatgatgc cggtcacctc
     1501 ggcgatcgag ccgctccgca tcgccaaccg gctatcggaa aaatcgctct accgctggac
     1561 catgcattcg gtcgacggca agccggtggc cgcctccaac ggcatcctgg cgatggtcga
     1621 tggcgatctg gcagtggtgc ccgagaacgc caccgtcatc atctgcggcg gcctgaacgt
     1681 gcagcatcac gccgacaagc gccttgtcgc ctggttgcgc aagatcgccc gcaagggccc
     1741 cgacatcggc gcagtctgca ccggtgccca tatccttgcc gaggccgggc tgctcgacgg
     1801 ctaccgctgc accatccact gggagaacct gccgggcttc tcggaggcct tccccgagat
     1861 cgacgccacc ggcggcctgt tcgagatcga ccgcgaccgt ttcaccagcg ccggcgggac
     1921 caccgcgctc gacatgatgc tgacgctgat cgccagccag catggcccgg agctcgcgtc
     1981 atcggtcgcc gaaggcatcc tgcatgcccc gatccgccat cattccgagc accagcgcat
     2041 gtcgctcccg gcccgaatcg gagcccgcca tcccaagctg gtcggcatca tcgaggagat
     2101 ggagggcaac ctggaagaac cgctctcgcc cagcctcctc gccagacagg ccggcctgtc
     2161 cacccgccag ctcgaacggc tcttccgccg ttacctggat cgctcgccca agcgctacta
     2221 tctcgagctg cggctgaaga aggcccgttc gctcctgctg cagaccgaca tgtcggtgat
     2281 caatgtggcg ctggcctgcg gcttctcctc gccgtcgcat ttctcgaaat gctatcgcgc
     2341 cttctacggc cgcaccccct accgcgagcg cggcataccg gtgatcgagg cggccgaaca
     2401 cgcctgatgc caatgagaaa gggcgggccg tttccggccc gccccgtcac cactcaggtt
     2461 ctggaccggg ggcgaccagc agaacctgac atgcagctga cttaaagtca tgatgctctg
     2521 aatctaagca ccaatgctta atgtagcata ggtccaacca ccttcatcaa caatcgattt
     2581 caaagacatg gtgaaacccg ccggtccacc tcaggctgca gctggtagga tcaggcgaac
     2641 acgaagacct cccagctcgg catcgtccag ctcgatgctg ccgccataca tctgggccgt
     2701 ttccttgacg atcgacaggc caaggcccga acccggcttg gtctcgtcca gccgctgacc
     2761 gcgcttcagc gcgtcggcgc ggcgctcggc cggaagaccc ggcccgtcat cgtcaaccag
     2821 cagcgtcagc caggtgcggc cctgatgcgc cgcagcgccg ccgagactgg ctttaaccac
     2881 cacgcgcttg gcggcccact tgcaggcatt gtcgatgaga ttgcccatca cctcctcgaa
     2941 gtcctgcttt tcgccccgga acttgtaggc gcccttgcat ttcaccttca ccgtaacgcc
     3001 cttgtcgcga tggatgcgtt cggtggtgcg ggcgatgcct tcgagcaccg gcttgatctc
     3061 ggtcaccgcg cctaagccct gggcgcgcgc ggcgcggcgt gcgcggtcca gatagaggct
     3121 cacgtgatcg cgcatcacat tcgtctgctc caccaccttt gaggcaaagg ggctcttgtg
     3181 cacgtgggct tcgttggtca aaacgctcag cggcgtcttc agggcatgcg cgagattgcc
     3241 gacctgggtt cgggcgcgct ctatgatgtc ggcgttcgac ttgatcaaca ggttgagttc
     3301 gtcggccacc gggcggatct cggcggggaa ttccccttcg agcttctccg ccctgccctc
     3361 gcggatcgcc gtcacgccgg cccgcaggcg cttgagcgga agcaggccat agttcacctg
     3421 cagcagcacg gcaaccatca gtccgattcc gagcaggccc agcaccacga acaaggtgcg
     3481 gcggaaggca tcgatctcgc ccttcagctc gtcgaagttt ccagcaacca gaaaggaata
     3541 gttccgttcg ttgtcgccga gcttgaagtt ctgctggatg acccgcagct gcgtgccgtt
//
LOCUS       DBNGVR010000150         5499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_839968, whole genome shotgun sequence.
ACCESSION   DBNGVR010000150 DBNGVR010000000
VERSION     DBNGVR010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_839968"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="AC16A0_03275"
     CDS             complement(<1..955)
                     /locus_tag="AC16A0_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3104381.1"
                     /translation="MAAAPRSTHVGGAEGPTDAAGIVKDLIITALLALAIFGPFVGLK
                     TVTASGTLALQQRWGEVAVFVAITVAGRLCIHLYQMMKPKAAAAAPAKAASAKPEFAI
                     GKYLGPIMLVIAIALPFIASTTGDRERQIMDLAILIMTYIMLGWGLNIVVGLAGLLDL
                     GYVAFYAVGAYTYALLATIYFPMWFGEGIIPWAFFITLPIAGILAALWGVILGFPVLR
                     LRGDYLAIVTLAFGEIIRVVLLNWVDFTGGPAGIGQIPRPSFFGLPFTADEGGFAAFF
                     GLEHKPIYRIIWLYYIILVLALLTNFGTLRLRKLPVGRAWEA"
     gene            complement(960..1895)
                     /locus_tag="AC16A0_03280"
     CDS             complement(960..1895)
                     /locus_tag="AC16A0_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3104382.1"
                     /translation="MEYFLQQLINGITLGSIYGLIAIGYTMVYGIIGMINFAHGDVFM
                     IGGFVSLIAIVALGLTGGSGMFLIILSLILVVIIAMIIAAAFNWTIERVAYRPLRGSF
                     RLAPLISAIGKSIVLQEYVRIAQDGRPKPLQPIVTGGHVIMERASDQGEFIVRLSNMQ
                     IFIIITTLVLMAVFTAIITRTSLGRAQRSCEQDMKMASLLGIDVNKTISITFIMGAAL
                     AAVAGMMFFLYYGVIDFYVGFLAGVKAFTAAVLGGIGSIPGAMLGGLIIGLIEVFWSA
                     YFSVEYKDVAAFAMLAAVLIFLPQGILGKPEVEKV"
     gene            2101..2637
                     /locus_tag="AC16A0_03285"
     CDS             2101..2637
                     /locus_tag="AC16A0_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3104383.1"
                     /translation="MNVRTLCLGFLSTGEASGYEIKKGIEDGMFSHFIDASFGSIYPA
                     LTQLTAEGLLTVRAEEQTGKPDKKVYAITEDGRKALARAISVLPAKDKFKSEFLFQML
                     LHQFITPDVMLAAIDKQLNDLRHDLARIAECRCSEQPHAGARFVADYGEAVLTASVRC
                     LEEKRRELVRQTAAQAAE"
     gene            2655..3797
                     /locus_tag="AC16A0_03290"
     CDS             2655..3797
                     /locus_tag="AC16A0_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3104384.1"
                     /translation="MIRRVFYLLLVTILLGGLAGAIAYYAFDFKPKMLATVILGAPRP
                     PETVAAEPARMDTWQPQISAIGTLTASEGIDITPQLGGLVEQINFESGQDVKKGQLLV
                     KLDTSTEEADIRSIEAELANNQTELTRKEGLVGKGIVARTELDALTTRKRVLQAGLDR
                     LTAIVAQKHIYAPWDGRVGLREISIGSYLAAGQKIVWMQKIDPIYADFQVTEAEFGRI
                     REGLKVTATFNAYPGESFTGEIATTDARVSNDSRMITVRASLPNGDLRLVPGMYAKVL
                     VDSGAPETVVTVPQTAVTYSLYGDNVFVVVPATKLDPNAKPEEKAIERRFVKAGPVRD
                     GRVQIVSGLADGEEVVTAGQNKIDQGSKVVIDNSIALKLQDTSTIQ"
     gene            3810..>5499
                     /locus_tag="AC16A0_03295"
     CDS             3810..>5499
                     /locus_tag="AC16A0_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3104385.1"
                     /translation="MHFTDIFVKRPVLATVVSLLILLAGLQAILNLPIRQFPEVSDTK
                     IEITTIYPGANADQIKGFITTPLQQAVASTEGVDTIESRSSQNSSVITLKLRLDADAD
                     RALADVLSKVNEVKGVLPEDANDPIVKRTTGAGFALMYISFKSEQMSSPQISDYLDRV
                     VRPRLQAINGVASAEILGGSTFAMRVWLDPEKMASLSITPLDVRNALAANNFTTAAGE
                     VKSDFTQQNINAQTSLETPEDFTDLVVATRDNTVIRLGQIATVDLGPENFNSKSAFDG
                     LKAVFMGIQTTPDANPLTVISAVRDAMPEIQRGLPSGLEANIAYDATEFINASIWEVG
                     KTLLEAGLIVIVVIFLFLGNFRSTIIPVITIPLSLVGVALVLVALGYSINLLTLLALV
                     LAIGLVVDDAIVVVENIHRHIEEGMKPFDAAIKGAREITGPIIAMTITLAVVYAPIGF
                     TTGLTGALFREFAFTLAGAVMVSGVVALTLSPMMTSKMLKPHAELSWFGRLVERVFGA
                     LQRFYRRRLDGTIKQRAVFAWTAVLTIALSAVLYNAINRELAPAEDQGVLFAFIN"
BASE COUNT         1134 a         1624 c         1730 g         1011 t
ORIGIN      
        1 gcgcctccca ggcgcggccc accggcaact tgcgcagacg caaggtcccg aagttggtga
       61 gcagggcgag gacaaggatg atgtagtaga gccagatgat gcgatagatc ggcttgtgtt
      121 ccagaccgaa gaaggccgcg aatccaccct cgtcagccgt gaaggggagg ccgaagaagg
      181 agggtcgcgg gatctggccg atgcctgcgg ggccgccggt gaagtccacc cagttgagca
      241 gcaccacgcg gatgatctcg ccgaaggcca gcgtcacgat ggcaaggtag tcgccgcgga
      301 ggcgcagcac cgggaagcca aggatgacgc cccacagcgc ggccaggatg ccggcgatcg
      361 gcagggtgat gaagaaggcc cagggaatga tgccttcgcc gaaccacatc gggaagtaga
      421 tggtggccag cagggcgtag gtataggcgc cgaccgcgta gaaggcgacg tagccgaggt
      481 cgagcagtcc ggcgagaccc accacgatgt tgagacccca ccccagcatg atgtaggtca
      541 tgatcaggat cgcgagatcc atgatctggc gttcgcggtc gccggtggtg ctggcgatga
      601 agggcagcgc aatcgcaatg accagcatga tcggaccgag atacttgccg atggcgaatt
      661 cgggtttcgc tgacgccgcc ttggccggag ccgcagccgc cgctttcggc ttcatcatct
      721 ggtagagatg gatgcaaagc cggcctgcaa cggtgatggc aacgaagacc gccacttcgc
      781 cccagcgctg ctggagggcg agcgtgccgc tggcggtgac cgtcttgagg ccgacgaagg
      841 ggccgaagat ggccaatgcc agaagcgcag taatgatcag gtccttgacg attcccgcgg
      901 catccgtcgg gccttcggca ccgccgacat gcgtggatct gggtgctgca gccatgatgt
      961 cagaccttct ccacttcggg tttgccgaga atgccctgcg gcaggaagat cagcacggcg
     1021 gcaagcatgg cgaaggctgc gacgtccttg tattcgacag agaagtaggc cgaccagaag
     1081 acctcgatca gaccgatgat caggccgccg agcattgcgc ctggaatcga tccgatgccg
     1141 cccagcacgg cggcggtgaa ggccttcacg ccggcgagga agcccacgta gaagtcgatc
     1201 acgccatagt agaggaagaa catcatgccg gccacggcag caagggcggc gcccatgatg
     1261 aaggtgatgg agatggtctt gttcacgtcg atcccgagca gcgaggccat cttcatgtcc
     1321 tgctcgcagg agcgctgggc ccggcccagc gaggtgcggg tgatgatcgc ggtgaagacc
     1381 gccatgagca ccagcgtggt gatgatgatg aagatctgca tgtttgacag gcggacgatg
     1441 aattcgcctt ggtcgctggc gcgctccatg atgacatggc cgccggtgac gatgggctgc
     1501 agcggctttg gtcggccgtc ctgcgcgatg cgcacatatt cctgcagcac gatggacttg
     1561 ccgatggccg agatcagggg tgccaggcgg aaggatccgc gcaggggccg gtaggccact
     1621 cgttcgatgg tccagttgaa ggccgccgcg atgatcatgg cgatgatcac caccaggatc
     1681 agcgagagga tgatcaggaa catcccgctg ccgccggtga ggccgagcgc gacgattgca
     1741 atgagcgata cgaagccgcc tatcatgaac acgtcgccgt gggcgaagtt gatcatgccg
     1801 atgattccgt agaccatggt gtatccgatg gcgatcaagc catagatcga ccccaaggta
     1861 attccattaa tcagctgctg gagaaaatac tccatgagcc cccctgcctg ttttctgtgc
     1921 ggcgctgttt ttgtgcacct gctaaagacc ttaatagaca gaagccccgg cgatgacaag
     1981 caaggtcggc tgcagtcaaa aattgtgatg acgccggcgg ttcttgtgtc ttgacactat
     2041 atcagttcga tatatatcga accggcattc atcgaaccag tgtcgtaacc cgaaatgacc
     2101 atgaacgtca ggacactctg ccttggattc ctctcgaccg gggaggcgtc gggctatgag
     2161 atcaagaaag ggatcgaaga tggaatgttc agtcatttca tcgacgcaag cttcggctcg
     2221 atctatccgg ccctgacgca actcactgcc gaagggctgc tgaccgtgcg ggcggaagag
     2281 cagaccggca agcccgacaa gaaggtctat gccatcacag aagatggtcg caaggccctt
     2341 gcacgggcaa tttccgtgct gcccgcgaag gacaagttca agtcggaatt cctgttccag
     2401 atgctgctgc accagttcat cacaccggac gtgatgctgg cggccatcga caagcagctg
     2461 aatgacctgc ggcacgatct agctcgtatt gccgagtgcc ggtgttcgga acagccacac
     2521 gcgggtgcgc gattcgtggc cgactatgga gaagcggtcc tgacagcctc tgtcaggtgc
     2581 ctggaagaaa agagacgcga gcttgtgcgc cagacagcgg cgcaggccgc cgaatagctg
     2641 acgggaacga gacgatgatc aggcgtgtat tctatctttt gctggtgacc atcctgctgg
     2701 gcggactggc tggcgcaatc gcctattatg ccttcgactt caagccgaag atgctggcaa
     2761 cggtgatcct cggcgctccg cgcccgccgg aaaccgtcgc cgccgaaccg gcacgcatgg
     2821 acacgtggca gccgcagatc tcggccattg gtacgctgac ggcatccgag ggcatcgaca
     2881 ttacgccaca gctgggtggc ctggtggagc agatcaattt cgaatccggg caagacgtca
     2941 agaagggcca gcttctggtc aagctcgaca cctcgaccga agaggcggac atccgcagca
     3001 tcgaggccga acttgccaac aaccagaccg agctgactcg caaggagggg ctcgttggga
     3061 agggcatcgt ggcgcggacg gaactcgacg cgctgaccac ccggaagagg gtcctccagg
     3121 cgggacttga tcgcctgacg gccatcgtgg cgcagaagca catctatgcg ccgtgggacg
     3181 gtcgtgtcgg cctgcgcgaa atctccatcg gcagctatct ggcggccggt cagaagatcg
     3241 tctggatgca gaagatcgat ccgatctatg ccgattttca ggtgaccgaa gctgaattcg
     3301 ggcggatccg cgagggattg aaggtgaccg ccaccttcaa cgcttatcct ggcgaaagct
     3361 tcaccggaga gatcgccacc actgatgcgc gcgtttcgaa cgacagccgc atgatcacgg
     3421 tgcgagcctc ccttccgaat ggcgatctca ggctggtgcc cggcatgtat gccaaggtgc
     3481 tggtcgattc cggtgcgcca gagacggtgg tgaccgtacc gcagacggcg gtgacctact
     3541 cgctttacgg cgacaatgtc tttgtggtcg tacccgcaac caagctggat cccaatgcga
     3601 agcccgaaga aaaggctatc gagcgccggt tcgtgaaggc cggtccggtc cgtgacgggc
     3661 gcgtccagat cgtcagcggc ctcgccgacg gcgaagaggt ggtgaccgct ggccagaaca
     3721 agatcgacca gggaagcaag gtggtgatcg acaactccat cgccctcaag ctgcaagaca
     3781 cgtcaacgat ccagtagagg ccgcccgcga tgcatttcac cgacatcttc gtcaagcggc
     3841 cggtcctcgc caccgtggtc agcctgctga ttcttctggc gggcctccag gccatcctga
     3901 acctgccgat tcggcagttc ccggaagtgt ccgacacgaa gatcgagatc accacgatct
     3961 atcctggcgc caatgccgat cagatcaagg gcttcatcac tacaccgctt cagcaggccg
     4021 ttgcctccac ggagggtgtg gacacgatcg aatcccgctc ttcgcagaac tcctcggtca
     4081 tcacgctgaa gcttcgcctc gatgccgatg ccgaccgggc cctggcagac gtattgtcaa
     4141 aggtgaacga ggtcaagggc gtcctgccgg aagacgccaa tgatccgatc gtgaagcgca
     4201 ccaccggcgc tggattcgcg ctgatgtaca tcagcttcaa gagcgagcag atgtcttcgc
     4261 cgcagatctc ggactatctg gatcgcgtgg tgcgtccgcg tctgcaggcg atcaatggcg
     4321 tggcctcggc ggaaattctt ggcggctcga ccttcgccat gcgcgtctgg ctcgacccgg
     4381 agaagatggc gtcgctctcg atcacgccgc tcgatgtccg caatgcgctt gcggccaaca
     4441 acttcaccac cgcggcgggc gaagtgaagt cggacttcac gcagcagaac atcaatgccc
     4501 agacctcgct tgaaacgccc gaggacttca ccgaccttgt ggttgccaca cgggacaaca
     4561 cggtcattcg cctcggccag atcgccacag tcgatctggg gccggaaaac ttcaattcca
     4621 agtcggcctt cgacggactg aaagccgtgt tcatgggcat ccagacgacg cccgatgcca
     4681 atccgctcac cgtcatttcc gccgtgcgcg atgccatgcc ggagattcag cgcggcctgc
     4741 catcaggcct ggaagccaac atcgcttatg atgccaccga gttcatcaat gcctcgatct
     4801 gggaggtcgg aaagactctg ctggaagctg gactgatcgt catcgtggtc attttcctgt
     4861 ttctcggcaa tttccggtcg acgatcatcc cggtcatcac cattccgctt tcgctggttg
     4921 gcgtggcgct ggtgctggtg gcgttgggct attccatcaa cctgctgacc ttgcttgccc
     4981 tggtgctcgc catcggtctg gtggtcgacg acgccatcgt ggtggtggag aacatccatc
     5041 gccacatcga agagggcatg aagccgttcg atgcggccat caagggcgcg cgcgagatca
     5101 ccgggccgat cattgccatg acgatcacac tggcggtggt ctatgctcca atcggcttca
     5161 caacaggcct caccggcgcg ctgttccgcg agtttgcctt cacgctggcg ggtgcggtga
     5221 tggtctcggg cgtcgtcgct ttgacgctgt caccgatgat gacgtcgaag atgctgaagc
     5281 cgcatgctga gctgagctgg ttcggccggc tggtcgagcg ggtcttcgga gccctgcagc
     5341 gcttctatcg ccgccgcctc gacggaacca tcaagcagcg cgcggtcttt gcctggaccg
     5401 cagtgctcac catcgcgttg tcggccgttc tctacaatgc gatcaaccgc gagctggccc
     5461 cggccgagga ccagggggtg ctctttgcct tcatcaatg
//
LOCUS       DBNGVR010000151         1619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2803406, whole genome shotgun sequence.
ACCESSION   DBNGVR010000151 DBNGVR010000000
VERSION     DBNGVR010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2803406"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="AC16A0_03300"
     CDS             complement(<1..545)
                     /locus_tag="AC16A0_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3104386.1"
                     /translation="MQTTAMDGLVALAAVPEADIPHTARIMADLSLFDCCVVALAGAH
                     APASCMVRDLVLDEDGRPAASLIGASRKVPPRAAALANGLTAHALDYDDTHFAHVGHP
                     SVAVLPAALAIAEDRGLSAAALRDAFLVGAEASIRLGVVLGQRHYDHGFHQTATAGCF
                     GASVAAGRLLDLGPQRLRMAL"
     gene            818..1570
                     /locus_tag="AC16A0_03305"
     CDS             818..1570
                     /locus_tag="AC16A0_03305"
                     /inference="COORDINATES: protein motif:HMM:NF039976.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HIR3104387.1"
                     /translation="MKLICYAVDPAYPVDIRPASQNRDWIDQTREQNAKRCLPLTAAN
                     SHGWEILAQDGCTVLWNGGADQSDLTVQTDSGTDVHIASHFGEGIVTFRPKCILRTEP
                     GYNIWVTGPVNGLKDGIQPLSGLLESDWMPYSFTMNWRVTRPGVEIRFERGEPICHFF
                     PVPRRIVDDAEPELRSIKDDPDLEAQHSAWFVSRKALVERLTNGEAMAPNETWQKLYH
                     RGLFPDGRVTAAGHQTRVRPQPFRSMPPKTQS"
BASE COUNT          308 a          534 c          511 g          266 t
ORIGIN      
        1 ctgagcgcca tgcgcagtct ttgcggcccg aggtccagca gccgccccgc ggccacgctc
       61 gccccgaaac atcctgccgt cgccgtctgg tgaaagccgt gatcgtaatg cctttgcccc
      121 agcaccacgc cgaggcggat cgaggcctcg gcccccacca ggaaggcgtc gcgcagcgcg
      181 gcggcggaaa ggccgcgatc ttctgcgatc gccagtgctg ccggcagcac cgccacggag
      241 ggatgcccga catgggcgaa atgggtgtcg tcatagtcca gggcatgcgc ggtgaggcca
      301 ttggcgagcg cagcggcgcg cggcggtacc ttgcgactgg cgccgatcag gctcgccgcc
      361 gggcgcccgt cttcgtcaag caccaggtcg cggaccatgc agcttgcggg cgcgtgggcg
      421 ccggcaaggg caaccacgca gcaatcgaag agcgacagat cggccatgat gcgggccgta
      481 tgcggaatat cggcctccgg cacggctgcc agggcgacaa ggccatccat cgcggtggtc
      541 tgcattcggt caatccaaca aaaaagaagg ggccgcgcaa gctggtcctc ccctgaggta
      601 ccgccggcat ccccaccgga cgatatcgaa tgaggatcgg accaaacaac ttcctctgct
      661 tgatcctgtg cttgggctgg agtgcaggca tcagcgtggc ggcccccaag ctctcgcagg
      721 ctcgtcatgg attgcaagaa atcccttgcg attgagacga attgcgattt gatccgatgt
      781 ccagctcaac ccctgtctgg agaggcgacg tcacggcatg aagctcatct gctacgcggt
      841 tgatcccgcc tatcccgtcg acatccggcc ggccagccaa aatcgcgact ggatcgacca
      901 aacgcgtgag cagaatgcga agcgatgcct gccgcttacg gccgccaatt cccatggctg
      961 ggaaatcctc gcgcaggacg gatgcacggt gctctggaac gggggcgccg atcagagcga
     1021 cctgaccgtt caaaccgact ccggcactga tgttcacatc gcgagtcact tcggcgaagg
     1081 cattgtcact ttccgcccca agtgcatcct gcggacggag cctggataca acatctgggt
     1141 cacggggccg gtcaacggac tcaaggacgg tatccagcct ctcagcgggc tgctggagtc
     1201 ggactggatg ccctacagct tcaccatgaa ctggcgtgtc acccggccgg gtgtcgagat
     1261 ccggttcgag cggggagagc cgatctgcca tttcttcccg gtgccgcggc gcatcgtgga
     1321 cgatgccgaa ccggaactcc ggtcgatcaa ggatgatccc gacctcgaag cccagcacag
     1381 cgcctggttt gtgtcccgca aggcgctcgt cgaacgcctg accaacggcg aagccatggc
     1441 gcccaatgag acgtggcaga agctctatca tcgcggactc tttccagatg ggcgagtgac
     1501 tgcggccggc caccagaccc gcgtcaggcc gcagccattc cggtccatgc cgcccaagac
     1561 gcagtcatga tggggctgcc tgtcatcgcc gatcggctca cgccatcgcc ttgcggagt
//
LOCUS       DBNGVR010000152         2454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3787160, whole genome shotgun sequence.
ACCESSION   DBNGVR010000152 DBNGVR010000000
VERSION     DBNGVR010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3787160"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC16A0_03310"
     CDS             complement(<1..431)
                     /locus_tag="AC16A0_03310"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR3104388.1"
                     /translation="MSLLVLGVVLLAGLHLIAAVPSQKQRLRQAVGAAAYGPVFGGAS
                     VVALAIIVLGWRMAPFVPVYDPPSWGWIANFALTLVAFICLGIFLFRGRLRQTLRFPM
                     GIAAIFWATGHLFANGDLASLILFGGMLVYAAAHIAIGMANG"
     gene            complement(428..1003)
                     /locus_tag="AC16A0_03315"
     CDS             complement(428..1003)
                     /locus_tag="AC16A0_03315"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090471 - 9,15,9'-tri-cis-zeta-carotene
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0016120 - carotene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR3104389.1"
                     /translation="MTYIYIGAILFGGMHLFSILFPSLRNAIRTSLGEKVWKIVYALV
                     SLAGIGFLIAGYWQSRSGPMTADILYDPPSWGRHLTMLMVLLAFILIGASHGKGYIKT
                     YVHNPMSWGIVLWSAGHLLANGQRSDVYLFGVFLIVGLADIIFSELRGKRPAHQPRLR
                     SDIIAVIVGMVLYVIFLFGFHPYVLNIPVAG"
     gene            complement(1038..1652)
                     /locus_tag="AC16A0_03320"
     CDS             complement(1038..1652)
                     /locus_tag="AC16A0_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3104390.1"
                     /translation="MARKAKLRPYHHGDLREALIASATEMIEEGGPSSFTLRECARRA
                     GVSHAAPKNHFATAEDLIAEVAARGFEHFVAALAKAADSSVDQSPDARLTAMCRAYVD
                     FARSHRGVYGLMFRERSGFAKSEHLRTAARAAWEQLHRSVAAVTGPGRGDTDAKAAHV
                     WSLVHGIASLTIDRRFPEIIDPDAVIATSVASLPAAIRSVGPAR"
     gene            1752..2174
                     /locus_tag="AC16A0_03325"
     CDS             1752..2174
                     /locus_tag="AC16A0_03325"
                     /inference="COORDINATES: protein motif:HMM:NF025473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abscisic acid-deficient protein Aba4 family
                     protein"
                     /protein_id="HIR3104391.1"
                     /translation="MTPELMFSLSGTVALVGWISLAAGILLRNALLRDTMAGLAIPLA
                     LSAAYAVLILVFWWTAEGGFDSLANVQKLFTSPWVALAGWIHYLAFDLAIGALLARKI
                     MAAGLSRLFLIPILPLTFLFGPIGFLLGQAVLLSRKEA"
     gene            2178..>2454
                     /locus_tag="AC16A0_03330"
     CDS             2178..>2454
                     /locus_tag="AC16A0_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104392.1"
                     /translation="MSDTIADRPTTASFLHRLRAASPLLWWTSLAFLTCFALCLVLQT
                     VDPRLLNGISVWIKPAKFFVSLALHMLTRAWGISLLPQTTRSGWTVRV"
BASE COUNT          458 a          789 c          792 g          415 t
ORIGIN      
        1 ccgttcgcca tgccaatggc gatgtgtgct gcggcataga caagcatgcc gccgaagagg
       61 atgaggctgg cgagatcgcc gttggcgaac aggtgtccgg tggcccagaa gatggccgcg
      121 atccccatgg gaaagcgcag cgtctgccgc agccggccgc ggaacaggaa aatgccgaga
      181 cagatgaagg ccacgagcgt gagagcgaag ttggcgatcc agccccagga cggcgggtcg
      241 tagacgggca cgaaaggcgc catgcgccag cccagcacga tgatcgcaag cgctaccacc
      301 gaggcgccac cgaagacggg gccataggct gcggcgccca cggcctgcct gagccgctgc
      361 ttctgcgacg gcacggcggc gatcagatgg aggcccgcga gcaggacgac gccaaggaca
      421 agcagactca tcccgcaacc ggaatgttga gcacgtaggg gtggaagcca aacaggaaga
      481 tcacgtagag caccatgccc acgatcaccg cgatgatgtc ggacctcagg cgcggctgat
      541 gcgccgggcg cttgccacgc aattcggaga agatgatgtc ggcgaggccc acgatcagga
      601 aaacgccaaa gagatagaca tcgctgcgct ggccgttggc cagcaggtga ccggccgacc
      661 acaggacgat gccccaggac atgggattgt gcacataggt cttgatgtag cccttgccat
      721 gcgaggcgcc gatgagaatg aaggccagca gcaccatcag catggtcaga tggcgccccc
      781 aggacggagg atcatagagg atgtcggcgg tcatcgggcc cgagcgcgac tgccagtagc
      841 cggcaatcag gaagccgatg ccggccagcg agacgagggc atagacgatc ttccagacct
      901 tctcgccgag actggtgcgg attgcattgc gcagcgacgg aaacaggatc gagaagagat
      961 gcatcccgcc gaacaggatt gcgccgatat agatgtaggt catgtgtgcc tctttgggtc
     1021 tgcgccttat gccagattca ccgcgccgga ccaaccgacc tgatggccgc aggcaggctg
     1081 gcgacgctgg tggcgatcac ggcgtccgga tcgatgatct cggggaaacg ccggtcgatg
     1141 gtgaggctgg cgatgccatg caccagcgac cagacatggg cggccttggc gtcggtgtcg
     1201 ccgcgtcccg gtcccgtcac ggccgcgacc gaccggtgca actgttccca ggcggcgcgt
     1261 gccgcggtcc gcagatgctc cgacttcgcg aagccgctgc gctcgcggaa catcaggcca
     1321 taaacgccgc gatgcgaccg ggcaaagtcc acataggcgc ggcacatggc ggtcagccgc
     1381 gcgtcgggtg actgatccac gctgctgtcc gcggccttgg ccagcgccgc gacgaagtgc
     1441 tcgaagccgc gcgccgcgac ttcggcaatc aggtcctcgg cggtcgcaaa gtgattcttg
     1501 ggcgcggcat gcgacactcc ggcccggcgg gcgcattccc gcagggtgaa ggacgacggg
     1561 ccgccctcct cgatcatctc ggtggcggag gcaatcaaag cctcgcgcag gtcgccgtga
     1621 tgatagggcc tgagtttcgc ctttcgcgcc atgtcacttc atttcacaaa atattgacgc
     1681 cgtcaaaatc aatattgaca atgtcaataa atacagcgca tgaatgttga caccgaaaac
     1741 ttggaggtcc catgacgcct gagctgatgt tctccctgtc cggcacggtg gcgctcgtcg
     1801 gctggatctc ccttgcggcc ggcatcctgc tccggaacgc cctgctgcgc gatacgatgg
     1861 ccggcctggc cataccgctg gcgctcagcg cggcctatgc cgtcctgatc ctggtcttct
     1921 ggtggacggc ggagggcggc ttcgactcgc ttgccaatgt gcagaagctc ttcacctcgc
     1981 cctgggtcgc actggccggt tggatccact acctcgcctt cgacctggcg atcggcgccc
     2041 ttctcgcccg caagatcatg gcggccggcc tgtcccgcct gttcctgatc ccgatcctgc
     2101 cgctcacctt cctcttcggg ccgatcggct tcctgctggg ccaggccgtg cttctcagcc
     2161 ggaaggaggc ctgatccatg agcgatacca tcgccgatcg ccccacgacg gcttccttcc
     2221 tccatcggct ccgcgcggca tcgccgctcc tgtggtggac cagcttggcc ttcctgacct
     2281 gctttgcgct ttgcctggtt ttgcagacgg tcgacccacg gctgctcaac ggcatcagcg
     2341 tgtggatcaa gccggcgaag ttctttgtgt cgctcgctct tcacatgctc acgcgggcct
     2401 ggggcatcag cctgcttccg cagaccacgc gcagcggctg gaccgtccgg gtca
//
LOCUS       DBNGVR010000153         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2215397, whole genome shotgun sequence.
ACCESSION   DBNGVR010000153 DBNGVR010000000
VERSION     DBNGVR010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2215397"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2433
                     /locus_tag="AC16A0_03335"
     CDS             <1..2433
                     /locus_tag="AC16A0_03335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HIR3104393.1"
                     /translation="PGRTPQSQLEHLAWEGAAERYPQGIPDKVRESLARELALIARLS
                     YAPYFLTVHDIVAFARQQEPPILCQGRGSAANSTVCYCIGITAVDPNQIELLFERFIS
                     EERKEPPDIDIDFEHERREEVIQYIYSRYGRERAGLAATVITYRGRSAVREVGKVMGL
                     SEDVTAALAGIVWGRMREEMQDGRLREAGLDPDDPLLARVIELAGELSGFPRHLSQHV
                     GGFVLTKGRLDETVPIGNAAMEDRTVIEWDKDDIDTLGILKVDVLGLGMLTCIRKALD
                     LLAAWYGQRYTLASVPREDKAVYDMLCKADSIGVFQVESRAQMNMLPRLRPRCFYDLV
                     IEVAIVRPGPIQGDMVHPYLRRRDGLEPEIYPAPHPDFGPADELRQVLGRTRGVPLFQ
                     EQAMKLAMVAAEFTPGELNELRKAMATFRRRDTIGRLEQKMVSRMVERGYDADFALRC
                     FNQIKGFGEYGFPESHAASFAHLVYVSSWLKCHHPDVFACALLNSQPMGFYAPAQIVR
                     DAREHGVTVLHPDVAYSSWDSTLEVPPEKSAFFALRLGLREVDGLRQADAARIVPGTI
                     ADMQRRGVPVPALEKLAAADCFRALGLDRRAALWEVKALARSKPLPLFAFAEVQDQGG
                     EPTVTLPAMPLSEHVVNDYQTLRLSLKAHPMSFLREACRAEGVIDNAQLKRKRNGANV
                     AVAGVVLVRQRPGSAKGVVFLTLEDECAVCNAVIWPQVLETHRAVVMGARLMLIRGRV
                     RRTGDIIHVVASRILDRSHWLARLAEDHAGFRAPLARADEVVRPGPDPAQTPAARHPR
                     QMRVIPRSRDFH"
BASE COUNT          396 a          898 c          847 g          402 t
ORIGIN      
        1 ccgggccgga cgccgcagtc gcagctcgag catctcgcct gggaaggcgc ggcggagcgc
       61 tacccgcagg ggattcccga caaggtgagg gagagcctcg ccagggaact ggccctcatc
      121 gccaggctca gttacgcgcc ttacttcctc acggtccacg acatcgtcgc cttcgcccgg
      181 caacaggagc cgcccatcct ctgtcagggc cggggatccg ccgccaattc caccgtgtgc
      241 tactgcatcg gcatcaccgc ggtcgatccc aaccagatcg agctgctctt cgagcgcttc
      301 atctcggaag agcgcaagga gccgcctgac atcgacatcg acttcgagca cgagcgccgc
      361 gaggaggtga tccagtacat ctattcccgc tacggccgcg aacgcgccgg cctcgccgcc
      421 accgtcatca cctatcgcgg acgctcggcg gtgcgcgagg tgggcaaggt catgggcctg
      481 tcggaagacg tcaccgcggc gctggccggc atcgtctggg gccgcatgcg cgaggaaatg
      541 caggacgggc ggctgcgcga ggcgggactc gatcccgacg atccgctgct cgcccgcgtc
      601 atcgaactgg cgggcgagct ctccggattt ccgcgccacc tgtcgcagca tgtcggcggc
      661 ttcgtgctga ccaagggccg gctcgatgag accgtgccca tcggcaatgc cgcgatggaa
      721 gaccgcaccg tcatcgaatg ggacaaggac gacatcgaca cgctgggcat tctcaaggtc
      781 gacgtgctgg gccttggcat gctcacctgc atccgcaagg cgctcgacct gctcgccgcc
      841 tggtacggcc agcgctacac gctggccagc gtgccgcgcg aggacaaggc cgtctacgac
      901 atgctgtgca aggccgattc gatcggcgtc ttccaggtcg agagccgggc ccagatgaac
      961 atgctgccgc ggctcaggcc gcgctgcttc tacgatctcg tcatcgaggt ggcgatcgtg
     1021 cggcccgggc cgatccaggg cgacatggtg catccttatc tgcgccgccg cgacgggctg
     1081 gagcccgaga tctatccggc cccccatccc gacttcggtc cggcggacga actgcgccag
     1141 gtgctgggcc gcacccgggg cgtgccgctg ttccaggaac aggcgatgaa gctcgccatg
     1201 gtggcggccg agttcacgcc gggcgagctg aacgagctgc gcaaggccat ggccaccttc
     1261 cggcggcgcg acaccatcgg ccggctggag cagaagatgg tgagccgcat ggtggagcgc
     1321 ggctatgacg cggatttcgc gctgcgctgt ttcaaccaga tcaagggatt cggcgaatac
     1381 ggctttcccg aaagccatgc ggcgagcttc gcccatctcg tctatgtctc gtcctggctc
     1441 aagtgccacc atcccgatgt cttcgcctgc gcgctgctca attcgcagcc catgggcttc
     1501 tatgcgccgg cccagatcgt gcgcgacgcg cgcgagcacg gcgtcaccgt actgcatccg
     1561 gacgtagcct attcgtcgtg ggattcaacg cttgaggtgc cgccggagaa gagcgccttc
     1621 ttcgccctgc gcctgggcct gcgcgaggtc gatggcctgc gccaggccga tgcggcgcgc
     1681 atcgtgcccg gcaccatcgc cgacatgcag cgccgcggcg tgccggtacc ggcgctggag
     1741 aagctggcgg cggccgactg cttccgcgcg ctgggcctcg accgccgcgc ggcgctgtgg
     1801 gaggtgaagg cgctggcgcg gtcgaagccc ctgccgctct ttgccttcgc cgaggtgcag
     1861 gaccagggcg gcgagcccac cgtgacgctg cccgccatgc cgctctccga gcacgtggtg
     1921 aacgactacc agacgctgcg cctgtcgctg aaggcgcatc ccatgtcctt cctgcgcgag
     1981 gcctgccggg ccgagggcgt catcgacaac gcgcagctca agcgcaagcg caatggcgca
     2041 aacgtcgcgg tggcgggcgt cgttctggtg cggcagcggc cgggctcggc caagggcgtg
     2101 gtcttcctca cgctggagga cgaatgcgcc gtctgcaacg ccgtgatctg gccgcaggtg
     2161 cttgagacgc accgcgccgt ggtcatgggc gcgcgcctca tgctgatccg cggccgggtg
     2221 cggcgcaccg gcgacatcat ccatgtggtg gccagccgca tccttgaccg cagccactgg
     2281 ctggcccgcc tcgccgagga ccacgccggc ttccgcgccc cgctggccag ggccgacgaa
     2341 gtggtccgcc ccggacccga tcccgcccag acacccgccg cccgccatcc ccgccagatg
     2401 cgcgtcatcc ccaggtcgcg cgacttccac tgaacccggg ccctcaggct tgcggcttga
     2461 tttgccgccg ggatatgcta gccagcgcag cacctcccgg tagctcagca ggatagagca
     2521 caggtttcct aaacctgggg tca
//
LOCUS       DBNGVR010000154         3955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1823633, whole genome shotgun sequence.
ACCESSION   DBNGVR010000154 DBNGVR010000000
VERSION     DBNGVR010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1823633"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1884
                     /locus_tag="AC16A0_03340"
     CDS             70..1884
                     /locus_tag="AC16A0_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HIR3104394.1"
                     /translation="MLDKVQAAQAETRSEGEESPDSTESTGPAAPQRGGFRHLIPRRL
                     TSRIVFLNLLGLVVLVAGILYFNQFRQGLIDARVQSLTTQAHIIAAAIAGSATVDTGS
                     IVIDPDSLSDTLDEARPDADQLSGLDFPINPETAGPILRRLLANTTVRARIVDPEGNL
                     VVDSRFLYSRGDIIQSELPPLAVDAGNPLKQLWDSFLTWAFSYDYPRQPEYGLDNGKD
                     FPEVAAALNGASVSLVRLNDRGQIVVLVSVPVQRYRAVLGALVLSTSGGEIDSVLGAE
                     RRVVFMTFGLVALVTILLSVFLAGTIAAPLRKLAAAAERVRRGVNKRVEIPDYTARQD
                     EIGELSGAIRDMTTSLYNRIDAIEAFAADVSHELKNPLTSLRSAVETLSFVKTDEQRA
                     RLIDIVKHDVKRLDRLITDISDASRLDAELARAELHAVDMVKLLTAIVDLANEVRKPD
                     QAEIQLDIARLPPGMDPVRGYMVVGQDSRLGQVVRNLLDNARSFTRPGTRLNVRMRRV
                     GDEVEFRVDDSGPGISPDNLERIFERFYTDRPEVSFGANSGLGLSISKQIVEAHKGRI
                     WAENRLGKADATGERTVLGARFIVRLPAVPEDWVPPNG"
     gene            1877..2344
                     /locus_tag="AC16A0_03345"
     CDS             1877..2344
                     /locus_tag="AC16A0_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HIR3104395.1"
                     /translation="MADSTTLHGTCVSVDGEGVLLLGLPGAGKSDLALRLVDQPGLGI
                     DGFLRVARLVADDQVVITRRGGDLQASAPATLKGKLEIRGLGIAALPAEEDVTLRMAV
                     RLERSADIERLPDLAGTRLDILGLGLPLVQIDPFAASAPARVRAALDLFRRPQ"
     gene            2421..2819
                     /locus_tag="AC16A0_03350"
     CDS             2421..2819
                     /locus_tag="AC16A0_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3104396.1"
                     /translation="MIGLVLVTHGRLAEEFLFALEHIVGPQTGIETISIGADDKMDAR
                     RVDIADAIGRADAGAGVIILTDMFGGTPSNLAISLMEIGRVEVVAGLNLPMLVKLARV
                     RKTSNLEKAAAAAQDAGRKYINIASQILGD"
     gene            2821..3093
                     /locus_tag="AC16A0_03355"
     CDS             2821..3093
                     /locus_tag="AC16A0_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3104397.1"
                     /translation="MSALSKEFAIINQRGLHARASAKFVKCAEGFDANITVTRDGQTV
                     PATSIMGLMMLAAAMGTSIIVEASGPQAEAAMAALEALVASRFDEE"
     gene            3150..>3955
                     /locus_tag="AC16A0_03360"
     CDS             3150..>3955
                     /locus_tag="AC16A0_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3104398.1"
                     /translation="MNQPRYQKLFEPVRIGPVTAPNRFYQTPHATGMGWRMPKASAAL
                     RGLKAEGGWGVVSTEYCSIHPSSDDFPFAYLSLWDEDDVRALAVTADAVHEHGSLLAV
                     ELWHGGFHCNNRLSRDAAVSPSGQALHLLGPGSARAMDKADIRALLGWQAEAAARARR
                     AGADIVYVYAGHDYLPLQFLSPRINRRSDTYGGSIANRTRLVREMIEVTRDAVHGTCA
                     VALRMSVDELHGPEGITHDGEAREIVALLAELPDLWDVNVSGALGNDSKSA"
BASE COUNT          664 a         1291 c         1258 g          742 t
ORIGIN      
        1 cccgacttcg acgccatcga gacgctctac ggcgttggct accgcttcaa ggaacagtaa
       61 cgggcggaga tgctggataa ggtgcaagcg gcgcaggccg agactcgctc cgaaggcgag
      121 gaaagtcctg actccaccga atccaccgga ccggcagcgc cgcaacgggg cggcttccgg
      181 catctcattc cgcgccgcct gacaagccgc atcgtcttcc tgaatctgct tggccttgtc
      241 gttctggtgg cgggcattct ttacttcaat cagttccgcc agggcctgat cgacgcgcgc
      301 gtccagagtc tgaccaccca ggcccacatc attgccgcag ccattgctgg ttctgcgacg
      361 gtggacacgg gatccatcgt catcgatcct gactcgctct cggatacgct cgacgaggcc
      421 agacccgatg ccgaccagct ctcgggactc gattttccaa taaatccgga aacggcagga
      481 cccattctcc ggcgactgct cgccaatacc acggtgcgcg cccgcatcgt cgatccagag
      541 ggcaatctcg tggtcgacag ccgctttctc tacagccgcg gcgatatcat ccagagcgaa
      601 ctcccgccac tagcggtcga tgccggcaat cccctcaagc agctctggga cagcttcctg
      661 acctgggcct tttcctacga ctatccgcgg cagccggagt atgggctcga caatggcaag
      721 gatttcccgg aagtcgcggc ggcactcaac ggcgcgtcgg tgagtcttgt ccgcctcaat
      781 gaccgcggcc agatcgtggt tctggtctcg gtgccggtgc agcgctatcg ggccgtgctt
      841 ggcgccctgg tgctgtccac ctctggcggc gagatcgaca gcgtgctcgg agcggaacgg
      901 cgcgtggtct tcatgacctt cggactggtg gcgctggtca ccatcctgct ctcggtcttc
      961 cttgccggta ccatcgcggc acccttgcgc aagctcgctg ccgccgccga acgtgtgcgg
     1021 cgcggcgtca acaagcgtgt cgagatcccg gactataccg cgcgccagga cgagattggc
     1081 gaactctcgg gcgcaatccg cgacatgacg acatcgctct acaaccgcat cgacgctatc
     1141 gaggccttcg ccgccgacgt gagtcacgag ctgaagaatc cgctgacatc gctgcgcagc
     1201 gccgtcgaaa ccctctcgtt cgtgaagacc gacgaacagc gcgcccgcct catcgatatc
     1261 gtcaagcatg acgtgaagcg gcttgaccgc ctgatcaccg atatctccga tgcgtcgcgt
     1321 ctcgatgcgg agctggcgcg cgccgagctt catgcggtcg atatggtgaa gctgctcacc
     1381 gccatcgtgg atctcgccaa tgaagtgcgc aagcccgatc aggccgaaat tcagcttgat
     1441 atcgcccgtc tcccgcccgg catggatccg gtgaggggct acatggttgt cggccaggac
     1501 tcccgccttg gtcaggtggt tcgcaatctg ctcgacaatg cgcggtcctt cacccgtcca
     1561 ggcacccgcc tcaacgtccg catgcggcgg gttggcgacg aggtggagtt ccgcgttgac
     1621 gactctggcc cgggcatcag tccggacaat ctggaacgga tcttcgagcg gttctatact
     1681 gatcggccgg aggtcagctt cggcgccaat tcgggcctcg gcctgtccat ctcgaagcag
     1741 atcgtcgagg cccacaaggg ccggatctgg gccgagaacc gcctgggcaa ggccgatgcc
     1801 accggcgagc gcactgtcct cggggcgcgc ttcattgtcc gcctgccagc cgttccggag
     1861 gattgggtgc cgcccaatgg ctgattccac cacgctccac gggacctgtg tctccgtgga
     1921 tggcgaaggg gttctgcttc ttggactgcc cggggccggc aagtcggacc tggccctgcg
     1981 ccttgtcgac cagccggggc ttgggatcga cgggttcctt cgcgttgcca ggctggtggc
     2041 cgacgatcag gtcgtcatca cccgcagagg cggggacctg caggccagcg cgcctgctac
     2101 actgaaggga aagctcgaga tccgtggcct gggcatcgcc gcgttgccgg cggaggagga
     2161 cgtgaccctg cggatggcgg tgcgcctcga gaggtcggcc gacatcgaac gcctgcccga
     2221 cctggcaggg acccgtctcg atattcttgg tctagggctg ccgttggtgc agatcgatcc
     2281 cttcgcggcc tccgcgccgg cccgggtgag ggcggcgctc gatctgttca ggaggccgca
     2341 atgacgtctt gcaagcgatc tccgatctgg gcatgttgcg cctccccgaa ctggccggag
     2401 catggagtct cagcgcaacg atgatcggtc tcgtcctggt cacccatggc cgcctggcag
     2461 aagagtttct ctttgcgctc gagcatatcg tcggacccca aaccgggatc gagacgattt
     2521 cgattggcgc cgatgacaag atggacgcgc ggcgcgtcga cattgccgat gcgatcggcc
     2581 gggccgatgc cggggcaggg gtgatcatcc tgaccgacat gttcggcgga acgccgtcca
     2641 atctcgcgat ttcactgatg gagattggcc gtgtcgaggt cgtcgccggc ctcaacctgc
     2701 cgatgcttgt gaagctggcg cgcgtgcgca agacctcgaa cctcgagaag gctgcagccg
     2761 ccgcgcagga tgcgggccgc aaatacatca acatcgccag ccagatcctg ggagactgac
     2821 gtgtcggcgc tatcgaagga gttcgccatc atcaaccagc gcggccttca cgcccgcgcc
     2881 tcggcgaagt tcgtgaagtg tgccgagggc ttcgacgcca acatcaccgt gacccgcgat
     2941 ggccagactg tccctgccac ctccatcatg ggtctgatga tgctggccgc ggcgatggga
     3001 acctcgatca tcgtcgaggc gtcgggcccg caggccgagg ccgcaatggc ggcgctcgag
     3061 gcgctggttg cctcccgctt cgacgaggaa taggcccgag gttgcgcttt tcaggccctg
     3121 caggcattgt gccgcagggg agtctgagcg tgaaccagcc gcgataccag aagcttttcg
     3181 agcctgtccg gatcggcccg gtgaccgcgc caaaccggtt ctaccagacg ccgcatgcca
     3241 ccggcatggg ttggcgcatg cccaaggcct cggccgccct gcgcggtctc aaggccgaag
     3301 gcggatgggg cgtggtctcc accgagtact gctctattca tccgtcatct gacgattttc
     3361 cttttgccta tctctcgctg tgggacgagg atgatgtccg cgcacttgcg gtcactgccg
     3421 atgccgtaca cgaacacggc agcctgttgg ccgtcgagct ctggcacggc gggtttcatt
     3481 gcaacaaccg actctcgcgc gatgcggcgg tctcgccatc gggccaggcc ctgcacctgc
     3541 ttggaccagg atcggcccgg gcgatggaca aggccgacat ccgcgccctt cttggttggc
     3601 aggcggaagc ggctgcgcgg gcgcgccgcg ccggtgccga tatcgtctat gtctatgctg
     3661 gtcacgacta tctgccgctg cagtttctct cgccccgcat caaccgacgc agcgacacct
     3721 atgggggatc gatcgcgaac cgcacccggc tggttcgcga gatgatcgag gtcacccgcg
     3781 atgcggtcca cggcacgtgt gccgtggcgc tccgcatgtc ggtggacgaa ctgcacgggc
     3841 ccgagggcat cacacatgat ggcgaggctc gcgagattgt cgcgcttttg gccgaacttc
     3901 ccgacctctg ggacgtgaat gtgagcggtg cgcttggcaa tgattcgaag agtgc
//
LOCUS       DBNGVR010000155         5887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2609807, whole genome shotgun sequence.
ACCESSION   DBNGVR010000155 DBNGVR010000000
VERSION     DBNGVR010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2609807"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="AC16A0_03365"
     CDS             <1..481
                     /locus_tag="AC16A0_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3104399.1"
                     /translation="TCLRVPVDIGIVEWTIPQLGAERLLGILRDRPDAPRIIVYAASN
                     DGEVPRRAMAAGAAGFCNRDLPQDQLLDMAATVAKGRMVFPYVDVRSLKRDPRESLTE
                     REKTMLAALARGRTNTELAADLGISINTVKFHLRNLYEKLGLRNRSQAIAFYYSSEA"
     gene            541..1638
                     /gene="mtnA"
                     /locus_tag="AC16A0_03370"
     CDS             541..1638
                     /gene="mtnA"
                     /locus_tag="AC16A0_03370"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR3104400.1"
                     /translation="MNVNGKAYRTIWPARDGWSVDIIDQTKFPHVFETVSLKTVEDAA
                     VAIRDMLVRGAPLIGVTAAYGVALAMHREASDAALAKAYDTLVVTRPTAINLKWALDE
                     MIKTLQPLRASDRRSAAYRRAAEIADEDVEINKAIGRNGFEIIKAIAAKKKPGERINI
                     LTHCNAGWLATVDWGTATAPIYTAHDAGLPIHVWVDETRPRNQGASLTAWELLHHGVP
                     HTVIPDNTGGHLMQHGLVDMAIVGTDRTTAQGDVCNKIGTYLKALAAHDNGVPFYVAL
                     PSPTIDFRVVDGVNEIPIEQRSGSEVSQMTGRTRDGRIETVEIVPEGSPVANYAFDVT
                     PARYVTGLITERGVIAANRVAIAKAFPERAG"
     gene            complement(1635..2921)
                     /gene="mtnK"
                     /locus_tag="AC16A0_03375"
     CDS             complement(1635..2921)
                     /gene="mtnK"
                     /locus_tag="AC16A0_03375"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046522 - S-methyl-5-thioribose kinase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HIR3104401.1"
                     /translation="MTTSIEQPEGYLPQSPSTLAAYLSCVAEVASILGGKPEDWTIRE
                     VGDGNLNLVFIVKGPLGGVAVKQALPYVRLVGESWPLPLTRAHYEHMALSEQARLTGS
                     LVPRVIHYDQLQALIVMELLEPHIIMRRGMIAATVYPKFAEDISRFMANTLFFTSDLA
                     LPADRKKKMIAAFAGNTALCKITEDLIFTDPYRVAELNRWTSPQLDGFARRWREDAEL
                     KIAVSRLKLKFLSSAEAMLHGDLHTGSVMLTADETRIIDPEFAFMGPMGFDVGAVIAN
                     FLINYLSHDGHEESPGARDAYRDWVLVTVEAIWTMFRSKFLDLWRGNGTGDAYPASLF
                     ADAAGAAALEAEREAYMERLWRDTIGFAAAKMIRRVLGLAHNIDLEWIKDPDVRASCE
                     ARVLTLARDLLVNTQHYPGIASVTAAARRIRTRKLL"
     gene            3222..4247
                     /locus_tag="AC16A0_03380"
     CDS             3222..4247
                     /locus_tag="AC16A0_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3104402.1"
                     /translation="MLNDLIKEAATRRDVFKKLGATALGAALLADAGFDPAMAQALAP
                     AGKPMKAAFSNAGLQATWCAQGKQAAEAWGKLMNVEITWFDGELAATKQRAAIDNMAS
                     QKWDFVAIQTFGIGTLTDPIKKMIDAGTPVIAMDTLVAPPGTVPIHTFIAPDNIMMGS
                     VVTSALVAAMGGAGNITMTQGVLGHSGAQGRAKGFHQVIDGNANIKLLDEQPADWDVT
                     KVARIWDSLLTQYPDIKGAFFHNDDMALAAHNVMKAKNRTDILIAGVDAMPPAVNAVL
                     EGVMVATVRNPSCRIHGWSVAAGVAAVQGGEQAGKDIPDFILADGPVITKDTAAGHLW
                     LQKNFLI"
     gene            4294..5778
                     /locus_tag="AC16A0_03385"
     CDS             4294..5778
                     /locus_tag="AC16A0_03385"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3104403.1"
                     /translation="MPPLLELEHVSKHFGGVMALRDVDFTLLPGEIHGLVGENGAGKS
                     TTMKIIAGVHTDFDGVMKIRGKEVRLRSARDALSNSIGMVHQELSIVPDLTVAENVFL
                     GNQPLTPLGTVDWSRMNREAKRLIASLGLEIDPTTTMGTLPVGLQQLVELSRVLFSGA
                     QIIILDEPTSALSPPEVKQLFAVLRRLRSEGRSIVFISHFLDDVLAISDRVTVFRNGQ
                     KVTTADTKTLTKDDVISHMIGRGSKGMHMGEEAELRGDDAKPIVLSVDGVSDGRSLRD
                     FSIELRAGEITGVYGFMGSGQIELSRALFGKLPLRRGTISLDGKPVRLRSTAAARAKG
                     IAFVPENRRMMLFRQEPVYKNVSISILDRIHRLFLKPQVERKITEGHIKDLQIRPPRT
                     GIALGSLSGGNQQKVALAKWLTHLPKVLILSEPTRGMDVGAKEDVIRIVKSLRDRGVA
                     VLVLSTEPETILTLADRVTVISKGMVAKRFSTGHIEKADLLAAA"
BASE COUNT         1149 a         1811 c         1864 g         1063 t
ORIGIN      
        1 gacctgcctg cgcgtccccg tcgacatcgg tatcgtcgag tggaccatac cgcagctcgg
       61 cgccgagcgg ttgctcggca tcctgcgcga ccggccggac gcgccgcgaa tcatcgtcta
      121 tgccgcatct aacgatgggg aagtgccgcg tcgggcgatg gcagccgggg cggccggatt
      181 ctgcaatcgc gacctgccgc aggatcagct gctggacatg gctgcgacgg tggccaaggg
      241 gcgaatggtg tttccctatg tcgacgttcg gagcttgaaa cgcgacccgc gcgaatcgct
      301 gaccgagcgc gagaagacca tgctggctgc gctggcccgc ggccgcacca atacggaact
      361 ggccgcagat ctcggcatct cgatcaatac ggtgaagttt caccttcgca atctgtatga
      421 gaagctaggg cttcgaaacc gctcgcaggc catcgccttc tactattcga gcgaagcttg
      481 acgggcgcgg cttgattgcc atgcgccgtc tggtagacct tgccgcccgg gagtgaatgc
      541 atgaatgtca acggcaaggc ctaccgcacc atctggcccg caagggacgg atggtctgtc
      601 gacatcatcg accagaccaa gtttccccat gtgttcgaga ccgtaagcct gaagacggtc
      661 gaggatgcgg ccgtcgccat ccgcgacatg ctggtcaggg gtgcgccgct gatcggtgtg
      721 acggcggcct atggcgtggc tctggccatg catcgcgagg cgtcagacgc cgcgttggcc
      781 aaggcctatg acacgctggt cgtaacgcgc ccgaccgcga tcaacctgaa gtgggcgttg
      841 gacgagatga tcaagacgct gcagcccctg cgtgcttcgg accggcgctc tgcggcctac
      901 cggcgcgcgg ccgagatcgc cgacgaggat gtcgagatca acaaggcgat cgggcggaac
      961 ggtttcgaga tcatcaaagc cattgccgca aagaagaagc cgggtgagcg catcaacatc
     1021 ctcacgcact gcaatgccgg ctggctcgcc accgtcgact ggggcaccgc caccgccccg
     1081 atctacacgg cgcatgacgc gggccttccc atccatgtct gggtggacga gacgcggccg
     1141 cgcaatcaag gcgcttccct gacggcctgg gagctcttgc atcacggcgt gccgcacacg
     1201 gtgattcccg acaacacagg gggtcacttg atgcagcacg ggcttgtcga catggcgatc
     1261 gtcggcaccg accggaccac ggcacagggg gatgtctgca acaagattgg cacctatctc
     1321 aaggcgctcg ccgcccatga caatggcgtg cccttctatg tggcgctgcc gtcgcccacc
     1381 atcgactttc gcgtcgtcga cggcgtgaac gaaatcccga tcgagcaacg gtccggcagc
     1441 gaggtctcgc agatgaccgg ccgcacccgc gatggacgga tcgagaccgt tgagatcgtt
     1501 ccggagggat cgccagtggc gaactacgcc tttgacgtga cgccggcacg gtatgtcacg
     1561 ggcctcatca ccgagcgtgg cgtcatcgcg gccaaccgcg ttgccatcgc gaaggcattc
     1621 cccgagcgcg ccggctagag cagcttccga gtccggatcc ggcgtgccgc agccgtcacc
     1681 gaagcgattc cgggataatg ctgcgtgttc accaacaggt cacgggcgag ggtcaggacg
     1741 cgggcctcgc aggatgcccg cacatcggga tccttgatcc attcgagatc gatgttgtgc
     1801 gcaagtccca gaacccggcg gatcatcttg gctgccgcga agccgatggt gtcgcgccag
     1861 agccgctcca tgtaggcttc gcgctctgcc tcgagtgccg ctgcgcccgc ggcatcggcg
     1921 aagagactgg cggggtaagc atcgcctgtg ccattgccgc gccagagatc gaggaacttg
     1981 gagcgaaaca tggtccagat cgcctcgact gtcacgagaa cccagtcgcg ataggcatcg
     2041 cgcgcgccgg gcgactcttc gtgaccgtcg tgggagaggt agttgatcag gaaattggcg
     2101 atcacggcgc ccacgtcgaa acccatgggt cccatgaagg cgaactccgg gtcgatgatg
     2161 cgggtctcgt ctgcggtcag catgaccgat ccggtatgca ggtcgccgtg cagcatggct
     2221 tctgccgagg acaggaactt cagcttgagc cgggacaccg cgatcttgag ttccgcgtcc
     2281 tcccgccagc gccgggcaaa cccgtcaagc tggggcgatg tccagcggtt cagttcagcg
     2341 acccggtaag gatcggtgaa gatgaggtcc tcggtgatct tgcagagtgc ggtattgccc
     2401 gcaaaggctg cgatcatctt cttcttccga tccgcgggca gcgccaggtc cgatgtaaag
     2461 aagagcgtgt tggccatgaa tctggaaatg tcttccgcga acttcgggta gacggtcgcc
     2521 gcgatcatgc cgcgccgcat gatgatatgc ggctccagca gttccatcac gatgagcgcc
     2581 tgcaactgat cgtagtggat gacacgcggt accagcgatc cggtcagtcg tgcctgttcg
     2641 ctgagggcca tgtgttcgta atgtgcgcgc gtgagcggca gtggccagct ctcgccgacg
     2701 agccgcacat agggcagggc ctgctttacc gcgaccccgc ccagcggtcc cttcacgatg
     2761 aagacaagat tgagattgcc gtcgcccact tcccgaatcg tccagtcctc gggctttccg
     2821 ccgagaatgc ttgcgacttc cgcgacgcat gacagatatg cggccagtgt ggatggagat
     2881 tgcgggaggt agccttccgg ctgttcaatg gatgtggtca tgtcatacgc gtgatcatgt
     2941 gtcgtgaccg tatcaagagc gaaaacggca cggcttgtcc agtgtgactc gccacattct
     3001 tgggcatgcc gtgcagtcgg cgcgccagcc gggcaatttc gcaaacgcag catcgattag
     3061 tgctttgcag caccatgaat tgtacgacaa aaggctagac aaaggcgtga agctgttgga
     3121 aggttccacg cagcaccgca caccaacgca aacatgcaat cgacctgtgc gcgcgcagac
     3181 aggtgctacg ccggcacggg acttggagga ggaccaagcc aatgttgaat gatctaatca
     3241 aggaagcagc gacccgccgt gacgtgttca agaaactcgg cgcgaccgcg ctcggcgcag
     3301 ccttgctggc cgatgccggt ttcgatcctg ccatggccca ggcactcgcc ccggctggca
     3361 agccgatgaa ggcggcgttc tcgaatgccg gcctgcaggc aacctggtgc gcccagggca
     3421 agcaggccgc ggaagcctgg ggcaagttga tgaacgtcga gatcacttgg ttcgacggcg
     3481 aactcgcggc caccaagcag cgcgccgcca tcgacaacat ggcctcgcag aagtgggact
     3541 ttgtcgccat ccagaccttc ggtatcggca cgctgaccga tccgatcaag aagatgatcg
     3601 acgccggaac gcccgtcatc gccatggaca cgctggtagc accgcccgga accgttccaa
     3661 tccatacctt tatcgcgccc gacaacatca tgatgggttc ggttgtcacc tcggcactgg
     3721 ttgcagcaat gggtggcgct ggaaacatta cgatgaccca gggcgtgctc ggccactccg
     3781 gtgcgcaggg acgggccaaa ggctttcatc aggtgatcga tggcaatgcc aacatcaagc
     3841 tgctggatga gcaaccggcc gattgggatg tgaccaaggt ggcccgtatc tgggattcgc
     3901 tgctcacgca gtatccggac atcaagggcg cgttcttcca caacgacgac atggctctgg
     3961 ctgcgcacaa cgtgatgaag gccaagaacc ggaccgacat cttgatcgcc ggtgtcgacg
     4021 ccatgccgcc ggcggtgaat gccgtgctcg aaggcgtgat ggttgccacc gtgcgcaacc
     4081 cgtcctgccg cattcatggc tggtcggtgg ccgccggtgt cgcggcggtg cagggcggcg
     4141 agcaggccgg gaaggacatt ccggacttca tcctcgccga cgggccggtc atcaccaagg
     4201 acacggctgc cggtcatctc tggctgcaga agaacttcct gatctgatcc ggagaccatg
     4261 acgggtcatt cgacagaaca gagcaggggc ggcatgccgc ccctgctcga acttgagcac
     4321 gtctccaagc acttcggcgg cgtcatggcg ctgcgcgacg tggacttcac gctgctgccc
     4381 ggcgagatcc atggactggt gggcgagaac ggcgccggca agtccacgac aatgaagatc
     4441 atcgccgggg tccacacgga tttcgacggc gtgatgaaga tccgcggcaa ggaagtgcgg
     4501 ctgcgctccg cccgggatgc gctctccaat tccatcggca tggtgcatca ggaactgtcc
     4561 atcgttccgg acctcacggt tgccgaaaat gtctttctgg gcaatcagcc cctgactccc
     4621 cttggcacgg tcgactggtc gcggatgaac cgcgaggcga agcgcctgat cgccagcctg
     4681 ggcctcgaga tcgatccgac caccacgatg ggaactctgc ccgtgggttt gcaacaattg
     4741 gtggaactct cgcgcgttct cttctcgggt gcgcagatca tcatcctcga cgaacccacc
     4801 tccgccttgt cgccgcccga agtgaaacaa ctcttcgcag tcctgcggcg tctgcggtcg
     4861 gaaggccgga gcatcgtctt catctcgcat tttctcgacg acgtgcttgc catctctgac
     4921 cgtgtgacgg tgttccgcaa tggccagaag gtgacgactg ccgataccaa gaccctgacc
     4981 aaggacgacg tcatctcgca tatgatcgga cgcggctcca agggcatgca catgggcgag
     5041 gaagcggagc tccgcggcga tgacgccaag cccatcgtgc tctcggtcga tggtgtgagc
     5101 gacggccgca gtctgcgcga tttctcgatc gaactgaggg ccggagagat cacgggcgtg
     5161 tacggtttca tgggatccgg acagatcgaa ttgtcccggg ccctgtttgg caagctccca
     5221 cttcggcgtg gcaccataag ccttgatggc aagcctgtgc ggctgaggtc aaccgcagcg
     5281 gcccgcgcca aggggatcgc ctttgttcct gagaaccgcc gcatgatgct gttccggcag
     5341 gaaccagtct acaagaacgt ctccatttcc attcttgacc gtattcaccg ccttttcttg
     5401 aaaccccagg tcgagcgaaa gatcaccgaa gggcacataa aggatcttca gatccgtccg
     5461 ccgcgcaccg ggatcgcgct gggttcactg tcgggcggca accagcagaa ggtggcgctg
     5521 gccaagtggc tgacccatct gcccaaggtg ttgatcctgt cggagcccac gcgtggcatg
     5581 gatgtcggcg cgaaggagga cgtgatccgg atcgtcaagt cgctgcgcga tcgcggtgtc
     5641 gcggtgctgg tcctgtccac ggaacccgag accattctca cgctggccga ccgcgtgacg
     5701 gtgatcagca agggaatggt tgccaagcgg ttttcgacgg gacatatcga aaaggctgac
     5761 ctgctggcgg cggcctgaga gcggaagagg ggatggacga cacatgacgg caaccgaagg
     5821 cgcagcaccg cgcaagttcg agctcaagcg gtttctcgcg gagcaactgc gcaacatcgc
     5881 tcccatc
//
LOCUS       DBNGVR010000156         2477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_645812, whole genome shotgun sequence.
ACCESSION   DBNGVR010000156 DBNGVR010000000
VERSION     DBNGVR010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_645812"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16A0_03390"
     CDS             <1..326
                     /locus_tag="AC16A0_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HIR3104404.1"
                     /translation="LLKPGAHVDLIGAYQPQMRESDDATIRRAGRIFLDTRLFCERSG
                     DIAEPLARGIVTREGLVADLFDLCCGRHRGRGSAAEITVYKNSGGGHLDLFTARHLYA
                     RVMAG"
     gene            complement(401..1762)
                     /gene="tig"
                     /locus_tag="AC16A0_03395"
     CDS             complement(401..1762)
                     /gene="tig"
                     /locus_tag="AC16A0_03395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3104405.1"
                     /translation="MQVTETLSSGLKREYKVVVNAAELDRELNSKLADLSKRANIKGF
                     RPGKVPVPHLKRVYGKSAMAEVVQKAIDDRSKALLEEKKLKPAYQPEVKLPEDEAEVN
                     AVMDGKGDLAFSLSFEVIPDFEVKDHSGLALVKHLVEVTEDHIAETLNRFASQSKSFE
                     EKQGEAANGDQLTISFVGTIDDTPFEGGTADDVQLELGSNQFIPGFEEQLIGAKAGEE
                     RTVRVTFPESYGVAELAGKPAAFAVKVTKVEAPKQAAIDEDFAKKMGFEDLAKLKDMI
                     KERMSQEFGQMTAMKLKRDVLDALDKLYDFELPVKLVEAEFNGIWTALTNEMKRAAKS
                     FADENTTEEDARKEYKAIAERRVRLGLVLGTLGEKEGIQVNEQELQQALMNRMRQFPG
                     QEKQVMDYYRKNPAALVELRGPIFEQKVVDLIVSRAKVDEKKVGKEDLQKMIEDEQDR
                     PEI"
     gene            complement(1817..1901)
                     /locus_tag="AC16A0_03400"
     tRNA            complement(1817..1901)
                     /locus_tag="AC16A0_03400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1865..1867),aa:Leu,seq:tag)
     gene            complement(1987..>2477)
                     /locus_tag="AC16A0_03405"
     CDS             complement(1987..>2477)
                     /locus_tag="AC16A0_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3104406.1"
                     /translation="GAIAIIVARNSGLDPGQSAWLLLAALVPVSLGLGNVYRTLAWPR
                     EAGPIRLAALTNLAAAGPLLALAWALDGLDLPALLAVPGLVGLQLLASSLMFLMFFRL
                     QQEGGPTYLSQIGYVAAAVGAGIGVGVLGESYPAGVWAGVAIVAAGIGLTTLAQLRTA
                     RA"
BASE COUNT          387 a          848 c          760 g          482 t
ORIGIN      
        1 cattgctgaa gcctggcgcg catgtcgatc tcatcggcgc ctatcagccg caaatgcgcg
       61 agtccgacga cgcgaccatc cgccgcgccg gacgcatctt cctcgacacg cgccttttct
      121 gcgagcgctc gggcgacatc gccgagccgc tggcccgcgg catcgtcacc cgcgaggggc
      181 tggtggccga cctcttcgat ctctgctgcg gccgccacag gggccgcggc tcagccgccg
      241 agatcaccgt ctacaagaat tccggcggcg gccatctcga cctcttcacc gcgcgccacc
      301 tctacgcgcg ggtcatggct ggctagctcg tccgtcgact gacgaaaacg gccgccccat
      361 gggcggccgc ttcgattcac gacgggtctc ctgagcgcgt tcagatctcg ggccggtcct
      421 gctcgtcctc gatcatcttc tgcaggtctt ccttgccgac cttcttctcg tccaccttgg
      481 cgcgcgagac gatgaggtcc accaccttct gctcgaagat ggggccgcgc agctcaacca
      541 gcgcagccgg gttcttgcgg taatagtcca tcacctgctt ttcctggccg gggaactggc
      601 gcatgcggtt catcagcgcc tgctgcagtt cctgctcgtt gacctggatg ccttccttct
      661 cgcccagcgt gccgagcacg aggccgaggc gcacgcggcg ctcggcaatc gccttgtatt
      721 ccttgcgggc atcctcttcc gtggtgttct cgtcggcgaa gctcttggcg gcgcgcttca
      781 tctcgttggt cagcgccgtc cagatgccgt tgaactcggc ctcgaccagc ttcaccggca
      841 gttcgaaatc atagagcttg tccagtgcgt cgagcacgtc gcgcttcagc ttcatggccg
      901 tcatctggcc gaactcctgg ctcatgcgct ccttgatcat gtccttgagc ttggccaggt
      961 cctcgaagcc catcttcttg gcaaagtctt cgtcgatggc agcctgcttc ggcgcttcca
     1021 ccttggtcac cttgaccgcg aaggcagccg gcttgccggc cagctcggcc acgccatagg
     1081 actcggggaa ggtcacccgg accgtgcgct cctctccggc cttggcgccg atgagctgct
     1141 cctcgaaacc ggggatgaac tggttcgatc ccagctccag ctgcacgtca tccgccgtgc
     1201 cgccctcgaa aggcgtgtcg tcgatggtgc ccacaaagct gatggtcagc tggtcgccat
     1261 ttgcggcctc gccctgcttt tcctcgaagc tcttggactg ggaagcgaag cggttcagcg
     1321 tctcggcgat gtggtcctcg gtcacctcga cgaggtgctt caccagcgca aggcccgaat
     1381 ggtccttcac ctcgaaatcg ggaataacct cgaaggacag cgagaaggcg aggtcgccct
     1441 tgccatccat cacggcgttc acttcggctt cgtcctcggg aagcttcact tccggctgat
     1501 aggcgggctt gagtttcttt tcctcgagca gcgccttgct gcggtcgtca atggccttct
     1561 gcacgacttc cgccatggcc gacttgccgt agacgcgctt cagatgcggc accggcacct
     1621 tgccggggcg gaagcccttg atgttggcgc gcttggacag gtcggcgagc ttggagttca
     1681 gttccctgtc gagctcggcg gcattgacca cgaccttgta ctcacgcttc aggccggagc
     1741 tcagtgtttc tgtgacttgc atatctctcg ctcttgtcta cgtcggtgtt tcgccaaggg
     1801 ggcatgggcc aggcgctggt gcgggcggag ggacttgaac ccccacgggt ttccccacag
     1861 gaacctaaat cctgcgtgtc tgccaatttc accacgcccg cgcggcagcc agtgccccgg
     1921 gttcgcattg ggcggggtct atagcagagg ggtttccgcg atgccagtgg agaaagtggg
     1981 gaagcttcag gcccttgccg tccgcaattg ggccagcgtc gtcagcccga tgcccgccgc
     2041 gacgatggca acgcccgccc agacgcccgc cggataggac tcgcccagaa cgccgacgcc
     2101 gatgcccgcg cccacggctg cggccacata gccgatctgg ctcagatagg taggcccgcc
     2161 ctcctgctgc agccggaaga acatgagaaa catgagcgaa gacgccagaa gctggaggcc
     2221 gaccagtccc ggcacggcga gcagcgcggg aaggtcgagg ccatccagcg cccaggccag
     2281 ggccagcaag gggcccgccg ccgccaggtt ggtgagcgcc gccagccgta ttggtccagc
     2341 ttccctgggc caggccaggg tgcggtagac attgccgagc ccgagagaca ccggcaccag
     2401 tgcggcaagc agcagccacg ctgactgtcc cgggtccagg ccgctgttgc gcgccacgat
     2461 gatcgcgatg gcgccca
//
LOCUS       DBNGVR010000157         3811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3395966, whole genome shotgun sequence.
ACCESSION   DBNGVR010000157 DBNGVR010000000
VERSION     DBNGVR010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3395966"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="AC16A0_03410"
     CDS             <1..1130
                     /locus_tag="AC16A0_03410"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR3104407.1"
                     /translation="AAFGDEGANTLGHIAERMPLKLPHMASLGLGQAAKLASGHDPLG
                     PATIIGRWGCATEISRGKDTITGHWEIAGVPLNRDWGYFPHSIPAFPQDLIDALVRRC
                     RLPGLLALCHASGTQVIEDFGEEHVRSGKPIVYTSVDSVIQIAAHEAHFGLDRLYEVC
                     RVARELTYPLNIGRVIARPFLGETAKTFVRTGHRKDYAVAPPSPTLLDALTAAGRPVI
                     SIGKIGDIFAHRGTGEEIKVAGLDTLMDVSLDAMDRLPDGGFAMTNFVDFDTEFGHRR
                     DPLGYGGLLEHYDTMLPRVFAKLRDSDLLVLTADHGNDPTWTGTDHTRERVPIMTYQR
                     GNRPGSLGARGSFADIGQSIAHHLGVAPLGAGKAWQAQGTS"
     gene            complement(1133..1840)
                     /locus_tag="AC16A0_03415"
     CDS             complement(1133..1840)
                     /locus_tag="AC16A0_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HIR3104408.1"
                     /translation="MFRLHSKRQVTFRCAAEDLGVIAEPVPAKQVLPEWFRKLPALDK
                     SHVTTADNGLTVKRCMPFLDAMMTGWIIPLAATVRLEVKDGGRTVNAGWEFDKVMVSN
                     HNPHQVAGSPTDPRPPCKFHNYWTIATPPGWSCLFVQPLNRHQPVFQLFSGVVDTDTY
                     QSLIHFPFVATAADGVYTLEKGTPLVQVIPFRREATGLQAEIRAETGDEADRRTRIMR
                     NTQSAEGWYREIARAKR"
     gene            1928..2461
                     /locus_tag="AC16A0_03420"
     CDS             1928..2461
                     /locus_tag="AC16A0_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104409.1"
                     /translation="MRFFLLALSSEELEELFELELLEEFDELLELELLEELDELFELE
                     LLEEFDELLELELLEEFDELFELELSEELSELLELELLEELPATRVNCSVSSAFACAC
                     WSMSAGTAAMAVPALIRAAPARVVTVIVNFFIGVSCLLVLAPMRSLHKDGAGTGLFPS
                     VFKTGMENRQRDTGRSN"
     gene            complement(2434..>3811)
                     /locus_tag="AC16A0_03425"
     CDS             complement(2434..>3811)
                     /locus_tag="AC16A0_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3104410.1"
                     /translation="LVLALGLFGLPGISTPVDVRTVLADDDGPGGDDDGGGGGGFGGG
                     DDDDDDRSNARTSRTQGSKFFSSGGSRNKAVKKSTTKKPAKKPAAKKKPRPVIVVLDL
                     PDSAIARLRRQGFAILSDNRLASTGIRVQRMRIPASMSLARANAIVKRLGASTSDVNS
                     YYTTQTLQGCERASDCDYRHLAGWSMDVIGACMASPRIGLVETHLEPKHPALLGQKIE
                     IIDVKPAERPRSGSSHGTGIAALLVGARESKAPGLLPDAELIVATPFYRAGNADRAEA
                     ADIIRAIDAVVSRQPDVLSLSLAGPPNAALARVLETVRARGIPVVAAAGNGGAKSKPL
                     YPAAYDTTIAVTAVTAKLNIFRRASRGDHVDFAAPGVEIAVADGKNGLTKRSGTSFAV
                     PFVTASLAVLRAQQPARTIDDMVGELAASAKDLGEPGKDSTFGWGLADASRLCAGPAD
                     QLLLPVSR"
BASE COUNT          659 a         1203 c         1214 g          735 t
ORIGIN      
        1 ccgcagcctt cggcgacgaa ggggcgaaca cgctgggcca cattgccgag cgcatgccgc
       61 tgaagctgcc gcacatggcc tcgctcggtc ttggccaggc ggcgaaactg gcctccggcc
      121 acgatccgct cggtcctgcc acgatcatcg gacgctgggg ctgcgccacc gaaatctcgc
      181 gcggcaagga caccatcacc gggcactggg agattgccgg cgtgccgctc aaccgcgact
      241 ggggttattt cccgcacagc atccccgcct ttccgcagga cctgatcgac gcgctggtgc
      301 ggcgctgccg gctgcctggc cttctcgcgc tctgccatgc ctcgggcacg caggtgatcg
      361 aggatttcgg cgaggagcac gtccgctccg gcaagcccat cgtctatacc agcgtcgaca
      421 gcgtcataca gatcgccgcg catgaagcgc atttcggtct cgaccgtctc tacgaggtct
      481 gccgcgtggc acgcgagctg acctacccgc tcaacatcgg ccgcgtgatt gcccggccct
      541 tcctgggcga gacggcgaag acctttgtcc gtactggcca tcgcaaggac tacgcggtcg
      601 cgccaccgtc gccgactctg ctcgacgcgc tgaccgccgc gggccggccg gtgatctcca
      661 tcggcaagat cggcgatatc ttcgcgcatc gcggcacggg cgaggagatc aaggtggccg
      721 gtctcgacac cctgatggac gtctcgctcg acgccatgga ccgcctgccc gatggcggct
      781 tcgccatgac caatttcgtc gacttcgaca cggagttcgg acatcgccgc gatcccttgg
      841 gttacggcgg cctgctggaa cactacgaca ccatgctgcc gcgggtcttc gcgaaactcc
      901 gcgatagcga cctgctggtg ctgacggccg atcacggcaa cgatccgacc tggacgggca
      961 ccgaccacac gcgcgagcgc gtgccgatca tgacctatca gcgcggcaac aggccgggca
     1021 gcctcggggc gcgcggcagc ttcgcggaca tcggccagtc catcgcccat caccttggcg
     1081 ttgcgccgct cggtgcgggc aaggcctggc aggcgcaagg gacatcatag actcagcgct
     1141 tcgcgcgcgc gatctcgcga taccagcctt cggcgctctg ggtattgcgc atgatgcggg
     1201 tacgccgatc ggcctcatcg ccggtctcgg cgcgaatctc ggcctgcaag ccggttgctt
     1261 cgcgccggaa gggaatcacc tgcaccagcg gcgttccctt ctccaacgtg tagacgccgt
     1321 cggcagcagt ggccacgaat ggaaaatgaa tgagggactg gtacgtgtcg gtgtcgacga
     1381 cacctgagaa aagctgaaac acaggctgat gccggttgag cggttgcacg aagaggcaac
     1441 tccaacctgg tggcgtcgcg atggtccagt agttgtggaa cttgcatggc gggcgtggat
     1501 cggtggggct gcccgcgacc tgatgcggat tgtgattgct caccatgacc ttgtcgaact
     1561 cccagccggc gttcactgtt cggccgccat ccttcacctc cagacggacg gttgccgcaa
     1621 gcggaatgat ccagcccgtc atcatggcgt cgagaaaggg catgcagcgc ttcacggtga
     1681 gaccattgtc tgccgtcgtg acatgggact tgtcgagtgc cggcagcttc cggaaccact
     1741 ccggaagaac ctgcttggcc ggcacgggtt cggcgatcac gccaagatct tccgctgcgc
     1801 aacggaaggt gacctggcgc tttgagtgaa gtcggaacat cctatggtcc tgttgatccg
     1861 ccgagacaag gagctggagg atgctggcct cggcatcctc caggcgttct gatgtcaggg
     1921 cttctgcttg cgcttcttcc tgttggcctt gtcgtccgaa gagttggaag agctgttcga
     1981 gctcgaattg ctcgaggagt tcgatgagct gttggagctg gagttgctcg aggaattgga
     2041 cgagctgttc gagctggaat tgctcgaaga gttcgacgag ctgttggagc tggagttgct
     2101 cgaggagttc gacgagctgt tcgagctcga gttgtccgag gagttgtccg agctgttgga
     2161 actcgagttg ctcgaggaat tgccggcaac ccgcgtgaat tgctcggtca gttcggcctt
     2221 tgcctgcgcc tgctggtcaa tgtcggccgg cacagcggcc atggcggttc ccgcactcat
     2281 cagagcggcg ccggcaaggg tcgtcaccgt catcgtgaac ttcttcattg gtgtctcctg
     2341 cttgttggtc ctcgctccga tgcggagcct acataaggac ggagcaggga ccggtttatt
     2401 cccgtcagtg ttcaaaaccg ggatggaaaa ccgtcaacgg gacacgggca ggagcaactg
     2461 atctgcagga ccggcacaca gcctggaggc atcggcaagt ccccatccga atgttgagtc
     2521 cttaccgggt tcccccagat ccttggccga ggctgcaagc tccccgacca tgtcgtcgat
     2581 cgtccgtgcc ggctgctgcg cacgcaagac ggccaggctc gccgtgacaa atggaacagc
     2641 gaatgatgtt ccggacctct tggtcaggcc gttcttgccg tcggcgactg caatctcgac
     2701 gccaggcgca gcaaaatcaa cgtggtcgcc acgagatgcc ctgcggaaga tgttcagttt
     2761 cgccgttacc gccgttacgg caatcgtcgt gtcataggct gcgggataga gaggctttga
     2821 ctttgcgccg ccatttccgg cggcggcaac aaccggaatc ccgcgggcgc ggacggtctc
     2881 gagaaccctt gcgagagccg catttggcgg gcccgccagg ctaaggctca acacgtcggg
     2941 ctggcgcgac accactgcgt cgatggcgcg aatgatgtcg gcggcttccg cccggtcggc
     3001 attgccggcc cggtagaacg gggttgccac gatcagttcg gcgtccggca ggagtccggg
     3061 tgccttgctt tcccttgcgc cgacaagcag tgcggcgatg ccggtgccat gactgcttcc
     3121 tgaccgcggc ctttcggctg gcttgacatc gatgatctcg atcttctggc ccagaagcgc
     3181 cggatgcttg ggttcgagat gcgtttctac caggccaatt cggggcgacg ccatgcaggc
     3241 cccgatcaca tccatcgacc atcccgccag atggcgatag tcgcagtcag acgcacgctc
     3301 gcagccctga agggtctgag ttgtgtagta gctgttcacg tccgatgtgg atgcgcccaa
     3361 ccgcttcacg atagcattgg cccgcgccag cgacatgctg gctggaatgc gcatgcgctg
     3421 aacgcgaatg cctgtgctgg cgagccggtt gtcgctaagg atcgcgaatc cctgccgccg
     3481 caaccgcgcg atggcagagt cgggaaggtc gagcacgacg ataacgggcc tgggcttctt
     3541 ctttgcggca ggcttctttg caggcttctt tgtcgtgctt ttcttgaccg ccttgttgcg
     3601 cgatccgcct gacgagaaga acttcgaacc ctgcgtccgg ctggttcggg cgttgctgcg
     3661 atcatcgtca tcgtcgtcac cgccgccgaa gccgccgcca ccaccaccgt catcgtcgcc
     3721 gccggggccg tcatcatcgg caaggacggt cctgacatcg acaggcgttg agatgcccgg
     3781 cagcccgaag aggcctagcg ccagaaccag c
//
LOCUS       DBNGVR010000158         2441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2021704, whole genome shotgun sequence.
ACCESSION   DBNGVR010000158 DBNGVR010000000
VERSION     DBNGVR010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2021704"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="AC16A0_03430"
     CDS             complement(<1..754)
                     /locus_tag="AC16A0_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104411.1"
                     /translation="MPSSSRLAGILAMVLSMGLFIASDSASKFAFDQVPVFQVVMLRA
                     LAAALLCTVIILALGQGGALAHAFNPWALARGLCEVGANLGFTLAILRLPIADVTAIA
                     QTAPLLVLLGASLIWGERIGPLRLVLIGIGVTGALLVAQPGTTAFSTYALLGFVVAAC
                     AATRDLLTRKVPPHVPAPVVALSVLLILTIAGTLGMVAFETPVMPDATSAVLLVAAGA
                     LMVGGHVGVFLAYKLASARSVAPFMYALTLWAV"
     gene            965..2230
                     /locus_tag="AC16A0_03435"
     CDS             965..2230
                     /locus_tag="AC16A0_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR3104412.1"
                     /translation="MTVEKTDTLVVGAGQAGLAMSEHLTKAGIPHLVLERHRIAERWR
                     SERWDSLVANGPAWHDRFPGLAFEGTDPDAFPHKEQVADYFVVYAEKFAAPVRCGVEV
                     MSVRKMVGRAGFRVETSQGAIEAKRVVAATGAFQRPVIPPLVPASAGIVQMHSTAYRT
                     PGQLPDGAVLVVGSGSSGAQIAEELLGAGWTVYLSVGPHDRPPRRYRGRDFVWWLGVL
                     GKWDAAAPAAGMEHVTIAVSGAHGGQTVDFRQLAARGMILVGRTEGFTDGVMRFAPDL
                     ARNITNGDANYLSVLDEADAFVTRNGLDLPEEPEARIIGPAPPCMTEPILELDLAKAG
                     VSSIIWATGFAADYSWLQVAAFDERGKPKHQRGVSSEPGVYFLGLPWQSRRGSTFIWG
                     VWHDAKYLADQIAIQRGYMQYSRESPARP"
     regulatory      2256..>2441
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          463 a          803 c          808 g          367 t
ORIGIN      
        1 gcacggccca gagcgtgagc gcatacatga aaggtgccac gctgcgggca gatgccagct
       61 tgtaggcgag gaagacgccg acatggccgc ccaccatcag cgcaccggcc gccaccagca
      121 agacggcact cgtcgcatcg ggcatgaccg gcgtctcgaa ggccaccatg ccgagcgtcc
      181 cggcaattgt caggatgagc aggaccgaca aggccacgac gggtgcggga acatgcggcg
      241 gcaccttgcg ggtgagaaga tcgcgcgttg ccgcacaggc tgccaccacg aagccaagca
      301 gcgcgtaggt cgagaaggcc gtcgtgccgg gctgtgcgac caggagcgcg cccgtgactc
      361 cgataccgat caacaccagc cgcagcgggc cgatcctttc gccccagatc aggctggcgc
      421 ccagcagcac caagagcggc gccgtctgcg caatggccgt gacatctgca atgggcaaac
      481 gcagaatcgc cagggtgaat ccgaggttcg ctccaacctc gcagagcccg cgcgccaggg
      541 cccagggatt gaaggcatgg gccagcgcgc cgccctgccc gagggcgagg atgatcacgg
      601 tgcagagaag cgccgcagcc agcgcgcgca gcatcacgac ctgaaagacc ggcacctggt
      661 cgaaggcgaa tttcgacgca ctgtcgctcg cgatgaagag gcccatggac aggaccatgg
      721 ccaggatgcc ggcaaggcgt gaagaagagg gcatgagacc gccgcggagg acaaccccca
      781 tctagccgcg gacgcgccgg acaacaacgg tcaacttcgg cgtgcggcca tgcgcgaacg
      841 atcttggttc ttgcgccacc cgcccaatcg ccgctttact ggagatcctc ccttgggtca
      901 ccagtctgac ctgcacgggc gaggaacggg gacaagagcc aaaggcggca agaaggcgaa
      961 tgacatgacg gtcgagaaaa ccgacacgct ggttgtcggc gcaggccagg cgggcctggc
     1021 gatgagcgag cacctgacca aggccggtat cccgcatctc gtgctggaac ggcaccggat
     1081 cgccgagcgc tggcgctcgg aacggtggga ttcgctggtc gccaacggtc cggcctggca
     1141 cgaccgtttt ccgggtcttg ccttcgaagg caccgatccc gatgcctttc cccacaagga
     1201 gcaggtggcg gactacttcg tcgtctatgc cgaaaagttt gcagcgcctg tccgctgcgg
     1261 agtcgaagtc atgtcggtcc gcaagatggt cggccgggcc ggcttccgcg tcgaaacctc
     1321 gcaaggcgcg atcgaggcca agagagtggt tgcggccacc ggcgccttcc aacgccctgt
     1381 cattccgccg ctggtgcccg cttcggccgg catcgtgcag atgcattcga ctgcctatcg
     1441 cactcccgga cagcttccgg atggtgctgt cctggtggtc ggctcgggct cttcgggggc
     1501 ccagatcgcc gaggaactcc tgggggccgg ctggaccgtc tatctgtcgg tggggccgca
     1561 cgaccggccg ccccgccgct atcgcggacg cgacttcgtc tggtggctcg gtgttctggg
     1621 aaagtgggat gccgccgcac ctgccgccgg catggagcat gtcaccatcg cggtaagcgg
     1681 tgcccatggc ggccagactg tcgacttccg ccagctggca gcgcgcggca tgatcctggt
     1741 gggccgcacg gaaggattca cagacggcgt catgcgcttc gcacccgatc tcgcgcggaa
     1801 catcacgaat ggtgacgcca actatctgtc ggtcctcgac gaagccgatg ccttcgtgac
     1861 gcgcaatggg ctggacctgc ctgaggaacc cgaagcccgt atcatcggtc ccgcaccacc
     1921 ttgcatgacg gaaccgatcc tcgaacttga tctggccaag gccggcgtct cctccatcat
     1981 ctgggccacc ggcttcgcgg ccgactacag ctggctgcag gtcgcggcct tcgacgaaag
     2041 ggggaagccg aagcaccagc gcggcgtttc gtctgaaccg ggagtttatt ttctcggcct
     2101 gccctggcag tcgcggcgcg gctcgacctt catctggggc gtctggcacg acgccaagta
     2161 cctcgccgac cagatcgcca tccagcgcgg ctacatgcag tacagccgag aatcacccgc
     2221 acggccgtag cctcttgtcg gattcgcctc gccagagtag cctcctgctc gatggtgccg
     2281 tggttggccg aagctgccgc ggctgaaaag ggaacatggt gaggaagacc ccagaggggc
     2341 cgaatccatg gctgcccacg caactgtcag cggagaggca tcctgcacat gccactggcc
     2401 ggaaggccgg gaaggcgcag gagaccggag acccgcgaag c
//
LOCUS       DBNGVR010000159         3015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_253169, whole genome shotgun sequence.
ACCESSION   DBNGVR010000159 DBNGVR010000000
VERSION     DBNGVR010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_253169"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="AC16A0_03440"
     CDS             complement(<1..390)
                     /locus_tag="AC16A0_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237447.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HIR3104413.1"
                     /translation="MDKKDVKATGQCPVMHGAQMLAAFGVRSNRDWWPNQLNLKMLHQ
                     HSSLSSPMARDFDYAKAFTSLDLKALKKDLQALMTDSQEWWPADFGHYGPLFIRMAWH
                     SAGTYRTGDGRGGAGSGQQRFAPLNSWP"
     gene            608..1561
                     /locus_tag="AC16A0_03445"
     CDS             608..1561
                     /locus_tag="AC16A0_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HIR3104414.1"
                     /translation="MINLTLRQFRYFEALRRHGHFGRAAEACAISQPALSMQIRELED
                     ALGTELFERGARQVRLTRLGEEFALRVRDILRSVDELEDIARASQDQLVGRLRIGIIP
                     TIAPYLLPTIIGNLTGAYAGLDIHVRETVTPRLIQELGEGRLDTAIVALPISEPSFTE
                     VALFSESFVLVRPGEDAGKPVPSRESLREMRLLLLEEGHCFRDQALSFCNLHAARPRE
                     LLDGSSLSTLVQMVSVGIGVTLIPEMAVAVETRSAPVSVAQFKPPRPSRTIGMIWRKT
                     SPLARQLLQMSEIVRQSAEDMRLRHAAAAVTPDSLSPAVGI"
     gene            1623..>3015
                     /locus_tag="AC16A0_03450"
     CDS             1623..>3015
                     /locus_tag="AC16A0_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3104415.1"
                     /translation="MAKAAAETRDIAEVVKSVLRGIWATLLTATLTVTFVISFTTIIY
                     GGSLAPLLETGLGLSLLGAALMCGVGGFFYSFRGTICQPQDVTAIVLAVTVSAMAETW
                     PADQEDSLMATIAVLVALTTAFSGILAWSFGMLRLGFLARFIPYPVIGGFLAATGFLL
                     LMGAISLGLGARVTVWNLPSLLAPGTLLLWLPWVLIGGGIAVAAHWFSREMLLPVVLA
                     LALAGFYAILYFAGMSLEQAEDASLLLGPFETGGFLQVLDPSLVLDADPWAILKAAPT
                     IAIVALLTILGTLLNATGLELALDRDLDLEKDLRATGIANIAGALGGGMTGYQTIPGT
                     VLGERLGLGSVLPGLSAAAGCLLIFIYGATELSFLPAGLFVAVIAYLGIDLLITWLWF
                     RRRQFSLTDYALVLLILAVAATVGFFQALGLGILVAAAMFIVSFAQVNVVRLRSTLAS
                     RRSLVERSDAEVHY"
BASE COUNT          454 a          979 c          931 g          651 t
ORIGIN      
        1 aggccagctg ttgagcgggg cgaagcgctg ctgccccgat ccggcgccgc cgcggccatc
       61 gccggtgcga taggtgccgg cactgtgcca ggccatgcgg atgaagagcg ggccatagtg
      121 gccgaagtcg gcgggccacc actcctgcga gtccgtcatc agggcctgca gatccttctt
      181 cagggccttg agatcgagcg acgtgaatgc cttggcgtag tcgaagtccc tggccatcgg
      241 gctggacagc gaggaatgct gatgcagcat cttgaggttc agctggttcg gccaccaatc
      301 gcggttggat ctcaccccga aggccgcaag catctgggct ccatgcatca ccgggcactg
      361 gccggtggcc ttgacgtctt tcttgtccat gcttctctcc aatcgtggtt gcgttgaccg
      421 atagacagag tctatctcat tgaatgtctt gaaagaaatt gcgagaaccc ggtccatgct
      481 agacgcccgg gaccgacctg tggagtctcc tttttgtcgc ctgttcccgc cttcgctgga
      541 ggtgtcttag caaagccagg cgattaattt aagctgaatt ttctgatcgc tgcgataaga
      601 tcggcttatg atcaatctga ccctcaggca attccgctat ttcgaggccc tgcggcgcca
      661 cggccacttc ggccgagccg ctgaggcctg cgccatttcg cagcccgcac tctccatgca
      721 gatcagggaa ctggaagacg cgctcggtac cgagctgttc gaaagaggcg cgcggcaggt
      781 gcgcctgact cgcctgggcg aggagttcgc gttgcgggtt cgcgatatcc tgcgttcggt
      841 tgatgaactt gaggatatcg cacgcgcctc ccaggatcag cttgtcgggc gcctgcgcat
      901 cggcatcatc cccaccatcg cgccctatct cctgccgacc atcatcggca atctgaccgg
      961 cgcctatgcc ggcctcgaca ttcatgtgcg cgagaccgtc acgccgcggc tcattcagga
     1021 gcttggcgaa ggccgccttg atacggcgat cgtggcactg cccatttcgg aaccctcctt
     1081 caccgaggtg gcgctcttct ctgaaagctt tgtcctcgtg cgtcccggcg aagacgcggg
     1141 aaaaccggtt cccagccgcg agtccctgcg cgagatgcgg ctcttgctgc tggaggaggg
     1201 ccactgcttc cgcgatcagg ccctctcttt ctgcaacttg catgcggcgc gcccgcgcga
     1261 actgctcgac ggcagttcgc tgtccacgct ggtgcagatg gtcagtgtcg gcatcggcgt
     1321 caccctcatt cccgagatgg cggtggcggt cgagacgcgg tcagccccgg tctccgttgc
     1381 gcagttcaag ccgccgcgcc cctcgcgcac catcggcatg atctggcgca agacgagtcc
     1441 tctggccagg cagcttctgc agatgtcgga gatcgtccgg cagtcggccg aagacatgcg
     1501 cctgcgtcat gccgccgccg ccgtgacgcc tgactcgctg tcacctgctg ttggaatcta
     1561 aatctcgaca aacccgttcg atttggattt gcttgcgccg caatcccggg gagacgtcac
     1621 aggtggcgaa agcggcagct gaaacgcgag acattgcgga agtggtgaaa tccgtcctgc
     1681 gcggaatctg ggcaacgctt ctcacggcca cgctcaccgt caccttcgtg atttccttca
     1741 ccaccatcat ttatggcggg tccctggcac ctctgctcga gacaggtctc gggctgtcgc
     1801 tcctgggtgc ggcgctgatg tgcggggtcg gcggcttctt ctacagcttt cgtgggacca
     1861 tctgccagcc gcaggacgtg acggcgatcg tgcttgcggt gaccgtcagc gccatggccg
     1921 agacctggcc tgccgatcag gaagacagcc tgatggcaac catcgccgtt ctggtcgcgc
     1981 tgaccacggc cttctcgggc atactggctt ggagcttcgg catgttgcgg cttggcttcc
     2041 tcgcacgctt cattccctat ccggtgatcg gcggatttct cgctgccacc ggctttctgc
     2101 tgctgatggg tgcgatcagc ctcggactcg gcgcccgcgt caccgtgtgg aatctgccct
     2161 cgctgcttgc acccggaacg ctgctgctct ggctgccctg ggtcttgatc ggcggcggca
     2221 ttgcggtggc tgcacattgg ttcagccggg aaatgctcct gccagtggtc ctggcgctcg
     2281 ccctcgccgg cttctacgca atcctctatt tcgcgggcat gagtctggag caggccgagg
     2341 atgccagcct cctgctcggt cccttcgaga caggcggctt tctgcaggtt ctcgatccct
     2401 cgcttgtgct tgatgccgat ccctgggcga tcctcaaggc agcgccaacc atcgcgatcg
     2461 tggcgctgct gaccattctc ggcacgctgc tcaatgccac gggtcttgag ctggcgctcg
     2521 atcgcgacct cgatctcgag aaggacctcc gcgcgaccgg catcgccaac attgccggtg
     2581 cgctgggcgg cggcatgacc ggctatcaga cgatccccgg aacggtgctg ggcgagcggc
     2641 tcggactggg cagcgttctg cccggcctca gcgcggccgc cggctgcctg ctgatcttca
     2701 tctatggcgc aacggaactg tcctttctcc cggccggact cttcgtcgcg gtgattgcct
     2761 atctcggcat tgatctcttg atcacctggc tctggttccg gcgccggcag ttctcgctca
     2821 ccgactatgc gctggtgctg ctgatcctcg cggtggcggc cacggtcggc ttcttccagg
     2881 cgctgggcct cggcatactc gtggcggccg ccatgttcat cgtgtccttc gcgcaggtga
     2941 atgtcgtgcg cttgcgctcg acgctggcca gccggcgttc gctggtcgag cgcagtgatg
     3001 cggaagtgca ctatc
//
LOCUS       DBNGVR010000160         3089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2415393, whole genome shotgun sequence.
ACCESSION   DBNGVR010000160 DBNGVR010000000
VERSION     DBNGVR010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2415393"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /gene="tatC"
                     /locus_tag="AC16A0_03455"
     CDS             <1..461
                     /gene="tatC"
                     /locus_tag="AC16A0_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0009977 - proton motive force dependent
                     protein transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3104416.1"
                     /translation="FRPYLIATPIFFLLGAGLVYFFLLPTAIHFFYTLAAGTGSEDQN
                     AIQLMPDIEAYLDFVMMLILAFGFCFQLPVILTLLGHIGVVTYDQLRSGRKFAIVGVF
                     IVAAIVTPPDPISQIAMAVPLMGLYEVSVQAVRFLESRRKKADAAREAEG"
     gene            524..1804
                     /gene="serS"
                     /locus_tag="AC16A0_03460"
     CDS             524..1804
                     /gene="serS"
                     /locus_tag="AC16A0_03460"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3104417.1"
                     /translation="MHDIKAIRDNPEAFDRGLARRGLAPMSLELIGIDAERRDHLQKL
                     QDAQARRNTVSKEVGDAMKAGDKARAEQLKAEVTSLKDFIASGEEAERQIDARLTAAL
                     AAIPNIPFDDVPDGKDETGNVEMRRWGEKPEFAFEPRQHFDIGEYLGLLDFESAAKIA
                     GARFAVMRGQLARLERALAQFMLDWQTGTNGYTENYVPYLVNDAAMFGTAQLPKFAED
                     QFRTTDGRWLIPTAEVSLTNLARESVLDEKDLPLRLTAYTPCFRAEAGAAGRDTRGMI
                     RQHQFSKVELVSITTPEASREELERMTSCAESVLQQLGLHYRVMRLCAGDMGFGSRMT
                     YDLEVWLPGQNAFREISSCSVCGDFQARRMEARYKPLDGKGTRYVHTLNGSGLAVGRT
                     LVAVLENYQNADGTVTIPEALRPYMGGAERITRP"
     gene            1804..2565
                     /gene="surE"
                     /locus_tag="AC16A0_03465"
     CDS             1804..2565
                     /gene="surE"
                     /locus_tag="AC16A0_03465"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3104418.1"
                     /translation="MRILVTNDDGIHAPGLEVLETIARNIADDVWVVAPAEENSGAGH
                     SLSLADPIRYRKLGERRFEVAGTPTDCVVMAVRKILPGMPDLVLSGVNRGQNIADDVT
                     YSGTIAAAMEGTLLGLKSIALSQVTGIHGNGFSYEVAAQYGTGVVKKLMAMTFGPGVL
                     MNVNFPDCRPDELQGVEVTRQGKRDVNLLTVDERVDARGNNYYWLGFKRERGNPAIGT
                     DLWAAFNKRISVTPLHLNLTQVEAMEAVRAALETP"
     gene            2562..>3089
                     /locus_tag="AC16A0_03470"
     CDS             2562..>3089
                     /locus_tag="AC16A0_03470"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3104419.1"
                     /translation="MTADPRKVALIMALRNQGVRDMRVLEAIEKVPRELFVEQPFLDQ
                     AYADQPLPIDCGQTISQPFIVGLMTDRLQLEERHKVLEIGTGSGYQTAILAQLCRRFF
                     TIERYRTLARQAEERFRALKLGNITQLVADGMKGWPQLAPFDRIIVTAAAAREVPPAL
                     LEQLAIGGIMIIPLEE"
BASE COUNT          540 a         1044 c          977 g          528 t
ORIGIN      
        1 ccttccgccc ctatctgatc gccacgccca tcttcttcct gctgggggcg gggctggtct
       61 atttcttcct gctgcctacg gccatccact tcttctatac gctggctgcc ggcaccggca
      121 gcgaggacca gaacgccatt cagctgatgc ccgacatcga ggcctatctc gacttcgtga
      181 tgatgctgat cctggccttc ggcttctgct tccagctgcc cgtgatcctc accctgctgg
      241 ggcacatcgg cgtcgtcacg tatgaccagc tgaggtcggg ccgcaagttc gccatcgtcg
      301 gcgttttcat cgtggcggcg atcgtgaccc cgcccgaccc gatttcgcag attgccatgg
      361 ccgtgccgct gatgggcctt tacgaagtct cggtccaggc cgtccgcttc ctcgaaagcc
      421 gccgcaagaa ggccgatgcg gcgcgcgagg ccgagggcta aacgccgcct ttgcctgagg
      481 ttcctcaagg ccttatagga gggcttcgca gaggaatcac gtcatgcacg acatcaaggc
      541 cattcgcgac aatccggagg cattcgacag ggggctggcc cgccgcgggc tggcgccgat
      601 gtcgctggag ctgatcggca tcgatgccga gcgccgcgac catctgcaga agctgcagga
      661 cgcgcaagcc cggcgcaaca cggtctccaa ggaggtgggc gatgcgatga aggcgggcga
      721 caaggcccgc gccgagcagc tcaaggcgga agtgaccagc ctcaaggact tcatcgcctc
      781 gggcgaggag gccgagcgcc agatcgatgc gcggctgacg gcggccttgg ccgccatccc
      841 caacatccct ttcgacgatg tgcctgacgg caaggatgag accggcaatg tcgagatgcg
      901 gcgctgggga gagaagccgg agtttgcctt cgaaccgcgc cagcacttcg acatcggcga
      961 gtatctcggg ctgctcgact tcgagagcgc ggcgaagatc gccggtgcgc gcttcgcagt
     1021 catgcggggg cagctggcgc ggctggagcg cgccctggcc cagttcatgc tcgactggca
     1081 gaccggcacc aatggctata ccgagaacta cgtgccctat ctggtgaacg acgccgccat
     1141 gttcggcaca gcgcagctgc ccaagttcgc cgaggaccag ttccgcacca ccgatgggcg
     1201 ctggctcatt cccaccgccg aagtatcgct caccaatctc gcgcgcgagt cggtgctcga
     1261 cgagaaggac ctgccgctgc ggctcaccgc ctacacgccc tgcttccgcg ccgaagcggg
     1321 cgctgccggg cgcgacacgc gcggcatgat ccgccagcat cagttctcca aggtcgagct
     1381 ggtctcgatc accacacccg aagcatcgcg cgaagagctg gagcgcatga cctcctgtgc
     1441 cgagagcgtg ctgcagcagc ttggcctgca ttaccgcgtc atgcggctct gcgccggcga
     1501 catgggcttc ggcagccgca tgacctatga cctcgaggtc tggctgcccg gccagaacgc
     1561 cttccgcgag atttcctcct gttcggtttg cggcgatttc caggcgcgcc ggatggaggc
     1621 ccgctacaag ccgctcgatg gcaagggcac ccgctatgtc catacgctga acgggtccgg
     1681 tctcgcggtc ggccggacgc tggttgctgt ccttgagaat taccagaacg ccgacggcac
     1741 tgtcacaatc cctgaagccc tgcgccccta tatgggcggc gcagagagaa tcacgaggcc
     1801 ctgatgcgca tccttgtcac caacgacgac ggcattcacg cccccgggct tgaggtgctt
     1861 gaaaccatcg cccggaacat cgccgacgat gtctgggtcg tggcgccggc ggaggaaaac
     1921 tcgggagccg ggcactcgct ctcgctggcc gatcccattc gctaccggaa gctcggtgag
     1981 cgccgcttcg aggtggccgg cacgcccacc gactgcgtgg tgatggcggt gcgcaagatc
     2041 ctgccgggca tgcccgacct cgtgctgtcg ggcgtcaacc gcggccagaa catcgccgat
     2101 gacgtcacct attccggcac catcgcggcg gcgatggaag ggacgctgct cggcctcaag
     2161 tccatcgcgc tgtcgcaggt gaccggcatc cacggcaacg gcttctccta cgaggtggca
     2221 gcacaatatg gcaccggcgt ggtgaagaag ctgatggcca tgaccttcgg ccctggcgtg
     2281 ctgatgaatg tcaattttcc cgattgccgc cccgatgaac tgcagggcgt agaagtgacg
     2341 cggcagggca agcgcgacgt gaacctgctc acggtcgacg agcgggtcga tgcgcgcggc
     2401 aacaactatt actggctcgg cttcaagcgg gagcgcggca atccggccat cggcaccgat
     2461 ctctgggccg ccttcaacaa gcgcatctcg gtcacgccgc tgcacctcaa tctcacgcag
     2521 gtcgaggcga tggaggcggt acgcgcggca ctcgagacgc cgtgaccgcc gatccccgca
     2581 aggttgccct gatcatggcc ctgcgaaacc agggcgtgcg cgacatgcgg gttctggaag
     2641 ccatcgagaa ggtaccgcgc gaactcttcg tcgagcagcc ctttctcgat caggcctatg
     2701 ccgaccagcc gctgcccatc gactgcggcc agaccatctc gcagcccttc atcgtgggcc
     2761 tcatgaccga tcgcctgcag ctggaagaac gccacaaggt gctcgagatc ggcacgggct
     2821 cgggctacca gaccgcgatc ctcgcccagc tctgccgccg ttttttcacc atcgagcgct
     2881 accgcacgct ggccaggcag gccgaggaac gcttcagggc gctgaagctc ggcaacatca
     2941 cgcagctggt ggccgacggc atgaagggct ggccccagct cgcgcctttc gaccgcatca
     3001 tcgtcacggc tgccgcagcc cgggaggtgc cgcctgcgct gctcgagcag ctcgccatcg
     3061 gcggcatcat gatcattccg ctggaggag
//
LOCUS       DBNGVR010000161         2459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3004517, whole genome shotgun sequence.
ACCESSION   DBNGVR010000161 DBNGVR010000000
VERSION     DBNGVR010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3004517"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16A0_03475"
     CDS             <1..333
                     /locus_tag="AC16A0_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3104420.1"
                     /translation="ARMAIDDYLARCPLSLKPHEPMFRGVRGGPLNARNIQLLIARLR
                     GALGLPETATPHALRHSFATHLLANGADLRVIQELLGHASLSTTQIYTEVNRAHLLEQ
                     YRKAHPRP"
     gene            330..1328
                     /locus_tag="AC16A0_03480"
     CDS             330..1328
                     /locus_tag="AC16A0_03480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR3104421.1"
                     /translation="MKRIALALAAVLFAGAAAAQEPPVCTGINLLDDLKSRDPATYEA
                     VMKEAAAVPNGEAILWKIEKDGVKPSHLLGTAHVTDPRVTKGPPGIEELIASSSKLAL
                     EVKELADDQEMAMAVMRSAPMMVMPVGKSLWDVIPDADEDAIRNSPNLPAGAAGTIFG
                     YQPWVVATMLTLPPCEQARDAAGLRSFDDLLARFASRKGVEVVGLETLEEQFAVMSGM
                     DMDLQARYLVETARMSDRAADQLETLLALYDRRQVTALMPLMLALEPPTETTKAVFAY
                     VESDLIRKRNHLMVKRAADLLAKGNVFIAVGALHLPGEDGLVELIRKAGYKVTPVN"
     gene            1341..>2459
                     /locus_tag="AC16A0_03485"
     CDS             1341..>2459
                     /locus_tag="AC16A0_03485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3104422.1"
                     /translation="MPVIDIAASDSRLPDRAGVAIIGGGVIGTATALELAERGIDVVL
                     LEKGDIAAEQSSRNWGWCRQMGRDPREIPLILESLDLWRRMDARIGAATGFTECGILY
                     LAETDAELEAKQSWYERHGRAYQLDIRVISGAETERLQPGASRAWKGALYSPTDGRAE
                     PFKAVPAMAAAVRARGGKVFTNCAVRGLETRGGRVSAVVTERGSIACDTVVLAGGAWS
                     RRFCHNLGIPLPQLSVVNSVMRTEPIDTGLTRSCSGGKFAFRKRADGGFTIAHRHLSV
                     ADIMPDSFRLFFDFLPLLKMDFGGVRLRLGRRFIEEARLKRRWALDEVSPFEQVRILD
                     PEPVPSILDEAGTSLMAYFPAFRGMKIAERWAGCIDATP"
BASE COUNT          410 a          848 c          774 g          427 t
ORIGIN      
        1 gcgcgcatgg ccatcgacga ttacctggcg cgctgtcccc tgagcctgaa gccgcatgag
       61 cccatgttcc gcggcgtgcg cggcggaccc ctcaacgccc gcaacatcca gctgctgatc
      121 gcaaggctgc gcggggcatt gggcctgccc gaaacggcga ccccccacgc cttgcggcac
      181 tccttcgcca cccatctgct ggccaatggc gcggacctcc gcgtgatcca ggaactgctc
      241 ggccatgcca gcctctcgac cacgcagatc tataccgaag tcaaccgcgc tcatctcctc
      301 gagcagtatc gcaaggcaca tccaaggcca tgaaacggat cgctctcgca ctcgctgccg
      361 tcctctttgc cggcgccgct gctgcccagg agccgccggt ctgcaccggc atcaatctgc
      421 tcgatgacct gaagtccagg gatcccgcga cctacgaggc ggtgatgaag gaggccgctg
      481 ccgtgcccaa tggcgaggcg atcctgtgga agatagagaa ggacggcgtc aagccctccc
      541 atcttctcgg caccgcgcat gtcaccgatc cgcgcgttac caaggggccg ccgggaatcg
      601 aggagctcat tgcctcctcc agcaagctgg cgctcgaggt gaaggaactg gccgacgacc
      661 aggaaatggc catggccgtc atgcgcagtg cgccgatgat ggtgatgccg gtcggcaaat
      721 ccttgtggga cgtcatcccg gacgcggacg aggatgcgat ccgcaacagt cccaacctgc
      781 ccgcgggcgc ggccggcacc atcttcggct accagccctg ggtggttgcc accatgctca
      841 ccctgccgcc ctgcgaacag gcccgtgacg cggccggcct gcggtccttc gacgatctcc
      901 ttgcccgctt cgccagccgc aagggcgtgg aggtcgtcgg cctcgaaacc ctggaagaac
      961 agttcgccgt catgtccggc atggacatgg acctgcaggc ccgctacctc gtcgagacgg
     1021 cgcgcatgtc ggatcgcgcc gccgatcagc ttgagacatt gctggcgctc tatgaccggc
     1081 ggcaggtcac cgccctcatg cccttgatgc tggcgctcga gccgccgacg gaaaccacca
     1141 aggccgtctt tgcctatgtc gaatccgatc tgatccgcaa gcgcaaccac ctcatggtca
     1201 agcgcgccgc cgatcttctc gcgaagggca acgtcttcat cgccgtgggt gcgctgcatc
     1261 tcccgggcga ggatggactg gtggaactga tccgcaaggc gggttacaag gtgactcccg
     1321 tcaactgagt gccgcctgcc gtgcctgtca tcgacattgc cgcttccgat tcccggcttc
     1381 cggacagggc aggcgttgcc atcatcggtg gcggcgtgat cggcaccgcc acggcacttg
     1441 aactcgccga gcgcggcatc gatgtcgtcc ttctggagaa gggcgatatc gctgccgaac
     1501 aatcgagccg gaactggggc tggtgccgcc agatgggacg cgatccgcgg gaaattccgc
     1561 tgatcctcga atccctcgat ctgtggcgcc gcatggacgc gcggatcggt gccgcaaccg
     1621 gcttcaccga atgcggcatt ctctatcttg ccgaaaccga tgccgaactc gaagccaagc
     1681 agagctggta tgaacggcac ggacgcgcct atcagctcga cattcgcgtc atatcgggcg
     1741 ccgagaccga gcgcctccag cccggcgcca gccgggcctg gaagggcgcg ctctattcgc
     1801 ccaccgacgg ccgcgccgaa ccctttaagg ccgtgccggc catggccgct gccgtgcgtg
     1861 cgaggggcgg caaggtcttc accaattgtg cggtgcgcgg cctcgagacc aggggcggac
     1921 gggtctcggc cgtcgtcacc gagcgggggt ccatcgcctg cgatacggtg gtgcttgccg
     1981 gcggtgcctg gtcgcggcgc ttctgtcaca atctcggcat tccgctgccg cagctctcgg
     2041 tggtcaattc ggtgatgcgc accgaaccca tcgacaccgg actcacgcgc agctgttccg
     2101 gcggcaagtt cgccttccgc aagcgcgccg acggcggctt caccatcgcg catcgccacc
     2161 tgtcggtcgc cgacatcatg ccggacagct tccggctctt cttcgatttc ctgccgttgc
     2221 tgaagatgga tttcggcggt gtgcgcctgc gcctcggcag gcgtttcatc gaggaggccc
     2281 ggctgaagcg gcgctgggcg ctggacgaag tcagcccgtt cgagcaggtc cggattcttg
     2341 atcctgaacc cgtgccatcg atcctggacg aggcaggcac gagcctgatg gcgtattttc
     2401 cggcgttccg cggcatgaag atcgccgagc gctgggccgg ctgcatcgac gcgacaccg
//
LOCUS       DBNGVR010000162         2601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4182205, whole genome shotgun sequence.
ACCESSION   DBNGVR010000162 DBNGVR010000000
VERSION     DBNGVR010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4182205"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1380
                     /locus_tag="AC16A0_03490"
     CDS             142..1380
                     /locus_tag="AC16A0_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3104423.1"
                     /translation="MFPQAANQNLAPLSGKLAENIMHFARVLREAGIPVGPGAVIDAL
                     DAAQCGSLRTREDFYWTLHAIFVKRRDQREVFDQAFHVFWKKPKMLEQLMQMFFHQVA
                     RDVGEKSKQAGFRRLAEAMFDKHEGKSQQRQKKDDLEIDSSFTASANEVLRRKDFEQM
                     TVAEQAQARQAIARLRLHRSEIMTRRYEPALKGSVIDMRRTLKGSLRAGGHFIDLERR
                     RRQWHEPPLVVLCDISGSCSNYSRMFLHFLHALSNDRDRVTVFLFGTRLTNITRELQR
                     RDIDEAMTKVSGAVKDWSGGTRIGTSLKEFNYKWARRVLTQGAQVLLMTDGLDREEGS
                     TLEHEMERLKRQTKRLIWLNPLLRFDGFRPLAGGIRAMMPHVDEFRPVHSLDSLSDLA
                     TALAGPPAAAHDPRKWMVRA"
     gene            1396..1719
                     /locus_tag="AC16A0_03495"
     CDS             1396..1719
                     /locus_tag="AC16A0_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3104424.1"
                     /translation="MSASDHSILEQASGWLQEGRKVAIATVVETWGSAPQPVGSQLVI
                     DAEGNFIGSVSGGCVEGEVVSEAVDIIATGKPRLLQFGVADETAWRVGLACGGRIGVY
                     VEPLA"
     gene            1716..2384
                     /locus_tag="AC16A0_03500"
     CDS             1716..2384
                     /locus_tag="AC16A0_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3104425.1"
                     /translation="MKRELLEAVLAARRERRAVALVTDLASGSQRLVARVEAARDPLG
                     AVLDEAFRFDQSGTHDGQFVNIHNPPLRLIIIGAVHIAQAVIPMAQQLGYDVTVIDPR
                     GAFATGARFPGVALFAEWPDEVIPRIGLDARTALIALTHDPKIDDPALNAALRSDLFY
                     IGALGSKKTQASRRARLVEAGFAEADISRIRGPIGLSIGAKGAPEIAVSIMAEMTRAL
                     RLGE"
     gene            2386..>2601
                     /locus_tag="AC16A0_03505"
     CDS             2386..>2601
                     /locus_tag="AC16A0_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HIR3104426.1"
                     /translation="MKFGELPVAEAEGAILAHSVRHGSGIFKKGRVLSLADISALRSA
                     GVDRVFAARLAADDVPEDKAAAAVAQVI"
BASE COUNT          467 a          816 c          854 g          464 t
ORIGIN      
        1 cacgctggat ccctcgaccg tcaacgatac gcttggcgtt ctgctgaaat atcaggacga
       61 catctcacgc atgcagggca ccgaggcgaa gcgcatcctc gaccagatca aggacgagat
      121 ccgcaccatg ggcaaggggt gatgtttccc caggccgcca atcagaacct ggcgccgctc
      181 tcgggcaagc tggcagagaa catcatgcat tttgcccgtg tcttgcgaga agcgggaatc
      241 ccggttggcc ccggtgccgt gatcgatgcg ctcgacgccg cgcaatgcgg atcgctcagg
      301 acgcgcgagg acttttactg gacattgcat gccatcttcg tgaagcgccg cgaccagcgc
      361 gaggtcttcg accaggcctt ccacgtcttc tggaagaagc cgaagatgct ggagcagctg
      421 atgcagatgt tcttccatca ggtggcccgc gacgtcggcg aaaagtccaa gcaggccggg
      481 ttccggcgcc tggcggaagc catgttcgac aagcacgagg gcaagagcca gcagcgccag
      541 aagaaggacg acctggaaat cgactcgagt ttcaccgcct cggccaacga ggtgctgcgc
      601 cggaaggact tcgagcagat gaccgtggcc gaacaggccc aggcacggca ggccattgcc
      661 cggctgcgcc tccaccgcag cgagatcatg acgcggcgct atgagccggc cctgaagggc
      721 agcgtgatcg acatgcggcg cactttgaaa ggctcgctgc gtgccggcgg ccacttcatc
      781 gatctcgagc gccggcgcag gcaatggcac gagccgccgc tggtcgtgct ctgcgacatc
      841 tccggatcat gttcgaacta cagccgcatg ttcctgcatt tcctgcatgc cttgtcgaat
      901 gaccgcgacc gggtgacggt gttccttttc ggaacgcggc tcaccaacat cacccgcgaa
      961 ctgcagcggc gtgacatcga cgaggctatg acgaaggtct cgggcgcggt gaaggactgg
     1021 tccggcggca cccgcatcgg cacgtcgctg aaggagttca actacaagtg ggcgcggcgc
     1081 gttctcacgc agggcgctca ggtgcttctg atgactgatg gcctcgaccg cgaggagggc
     1141 tcgacattgg aacacgagat ggaacggctg aagcgacaga ccaagcgcct catctggctc
     1201 aatccgctgc tgcgctttga tggcttcagg ccgctggcag gcggcatccg cgcgatgatg
     1261 ccgcatgtcg atgagttccg tccggttcac tcgctcgaca gcctgagcga cctcgccaca
     1321 gcccttgccg ggccgccggc agcggcgcat gatccgcgca agtggatggt gcgcgcttag
     1381 accaaggaga ttgccatgtc cgcctccgat cattccattc tcgaacaggc gtccggctgg
     1441 ttgcaggaag ggcgcaaggt cgctatcgca acggtggtcg aaacctgggg ctcggccccg
     1501 cagccggtcg gaagccagct ggtgatcgat gccgagggaa acttcatcgg gtcagtctcc
     1561 ggaggctgcg tcgaaggcga agtggtctct gaagccgtcg acatcatcgc cacgggcaag
     1621 cccaggctgc tccagttcgg cgtcgccgac gagacggcct ggcgcgtcgg gctggcctgc
     1681 ggcggccgca tcggcgtcta tgtggaaccg ctggcgtgaa gcgcgaactc ctcgaagctg
     1741 ttctggctgc gcgccgtgag cggcgggccg ttgcacttgt taccgatctg gcctcgggaa
     1801 gccagcgcct ggtggcccgc gtcgaggccg ctcgggaccc gctcggagcc gtcctggacg
     1861 aggcgtttcg cttcgatcag tccggaaccc acgacgggca gttcgtcaat attcacaacc
     1921 cgccgctgcg attgatcatc atcggcgccg tccatatcgc ccaggccgtg attccgatgg
     1981 cccagcaact cgggtatgac gtcaccgtga tcgacccgcg cggggccttc gcgaccggtg
     2041 cacggtttcc cggtgttgcg ctcttcgccg aatggcccga cgaggtcatt ccgcggatcg
     2101 gcctcgatgc ccgcacggcg ctgatcgcgc tgacccatga tccgaagatc gacgatcctg
     2161 cgttgaacgc ggcgttgcgc tcggacctct tctacatcgg cgccctgggc tcgaagaaga
     2221 cccaggcctc gcgacgagcc cgtctggtcg aggctggttt tgcagaagcc gatatctcgc
     2281 gcattcgcgg tcccatcggg ctcagcatcg gcgccaaggg ggcgcctgaa atcgccgtgt
     2341 cgatcatggc ggagatgacg cgggcgctgc gcctcggaga gtgagatgaa attcggagaa
     2401 cttccggttg cggaggccga aggcgccatt ctcgcccatt cggtcaggca tggcagcggc
     2461 atattcaaga aggggcgggt gttgtcgctg gctgacatct ctgctcttcg atctgccggc
     2521 gtcgacaggg tttttgctgc acggcttgca gcggatgatg tgccggagga caaggctgcc
     2581 gcagccgtgg cgcaggtcat c
//
LOCUS       DBNGVR010000163         2086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3792202, whole genome shotgun sequence.
ACCESSION   DBNGVR010000163 DBNGVR010000000
VERSION     DBNGVR010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3792202"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="AC16A0_03510"
     CDS             <1..875
                     /locus_tag="AC16A0_03510"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3104427.1"
                     /translation="DKAQAWSKIVKIGRTHTQDATPLTLGQEFSGYTQQVENGIARIE
                     QTMPMLMELAQGGTAVGTGLNAPVGFAELVADRIATITGMPFTSAPNKFEALAAHDAM
                     VMSHGAINTAAVSLFKIANDIRLLGSGPRSGLGELALPENEPGSSIMPGKVNPTQCEA
                     LTMVCVQVFGNQSAITFAGSQGHFELNVYNPVMAYNFLQSVRLLADAVISFTDNCVTG
                     IVAREDNIKAALERSLMLVTALAPKYGYDRAAKIAKTAHKNGTTLREEAIRDGIDAED
                     FDRIVRPEDMIGPK"
     gene            891..1061
                     /locus_tag="AC16A0_03515"
     CDS             891..1061
                     /locus_tag="AC16A0_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HIR3104428.1"
                     /translation="MGEIVNLKQQRKRRARDAREEKAAENRVSFGLSKKDRKRADTLR
                     DKAAKDLDGHKR"
     gene            1067..1285
                     /locus_tag="AC16A0_03520"
     CDS             1067..1285
                     /locus_tag="AC16A0_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR3104429.1"
                     /translation="MAKDLKHSLTIAGHRTSLSLEPEFWTALQKAAARRNLTIAGLVS
                     EIDQSRAGRNLSSAIRVWILNAVMTGLL"
     gene            complement(1277..>2086)
                     /locus_tag="AC16A0_03525"
     CDS             complement(1277..>2086)
                     /locus_tag="AC16A0_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104430.1"
                     /translation="AVDAARDGNGLTAAFDLLRKGQALGFGYVAGPVTISDGAVRFAP
                     FVATTPQAKVEVKVVGELALNAIDAEIAVAFPGRAGLPGLSIAYAGVPDALVRSEDMA
                     ELSTRLGVTIMQQGIDELERLQREQERLAREEEQQRKLDEERLQAYYAQRDELILRRR
                     ELRIHSEMRAYESERMRQKIEADREANAAISKDELRQRMRELKTFRQQAKLTRAAIPV
                     QVVRPKPKAAIQRQNVPKPKKPAAQQGPVILVQPEGAPVVISPPPSGSPSQ"
BASE COUNT          376 a          710 c          666 g          334 t
ORIGIN      
        1 acgacaaggc tcaggcgtgg tcgaaaatcg tcaagatcgg ccgcacccac acgcaggacg
       61 ccacgccgct gacgcttggc caggagttct ccggctacac ccagcaggtg gagaacggca
      121 tcgcgcgcat cgagcagacc atgcccatgc tgatggagct ggcgcagggc ggcaccgccg
      181 tcggcaccgg cctcaatgcg cctgtcggct tcgccgaact ggtggcggac cgcatcgcga
      241 ccatcaccgg catgcccttc acctcggcgc ccaacaagtt cgaggcgctc gccgcccatg
      301 atgcgatggt catgtcgcac ggcgccatca acaccgccgc ggtctcgctc ttcaagatcg
      361 ccaacgacat ccggctcctt ggctccggtc cgcgctcagg cctcggagaa ctggctctcc
      421 ccgagaacga gcccggctcg tcgatcatgc cgggcaaggt caatcccacc cagtgcgagg
      481 ccctgaccat ggtctgcgtg caggtcttcg gcaaccagtc ggccatcacc ttcgccggca
      541 gccaggggca tttcgaactc aacgtctaca atccggtgat ggcctacaac ttcctgcagt
      601 cggtgcgcct gctggccgat gcagtcatca gcttcaccga caattgcgtc acgggcatcg
      661 tggcgcgcga ggacaatatc aaggcggcgc tcgagcgttc gctcatgctg gtcacggcgc
      721 tggcgccgaa gtacggctat gaccgtgccg ccaagatcgc caagacggcg cacaagaacg
      781 gcaccacgct gcgcgaggaa gccatccgcg acggcatcga tgccgaggat ttcgaccgca
      841 tcgtgcgtcc ggaagacatg atcggaccga agtagacggg gacagggatg atgggcgaaa
      901 tcgtcaacct caagcagcaa cgcaagcgtc gcgcgcgcga cgcccgagaa gagaaagccg
      961 ccgagaaccg cgtcagcttc ggcctcagca agaaagaccg caagcgcgca gacaccttgc
     1021 gcgacaaggc ggcaaaggat ctggacggac acaagagata agcatcatgg caaaggacct
     1081 gaagcactcg ctgaccattg cgggccaccg cacctcgctg tcgctcgagc cggaattctg
     1141 gacagcgctc cagaaggccg ccgccaggcg caacctgacc atcgccgggc tggtgtcgga
     1201 gatcgaccag agccgggccg gccgcaacct gtccagcgcc attcgcgtct ggatcctcaa
     1261 tgcggtgatg accggcctat tgtgacggcg agccggatgg cggcggcgag atgacgaccg
     1321 gcgcgccttc cggctgcacc aggatcaccg ggccctgttg cgcggccggc ttcttcggtt
     1381 tcggcacgtt ctgcctctgg atggctgcct tgggcttggg ccgcaccacc tggaccggta
     1441 tagcggcgcg cgtcagcttc gcctgctggc ggaaggtctt gagctcgcgc atccgctgtc
     1501 gcagctcatc cttgctgatg gccgcattgg cctcccggtc cgcttcgatt ttctgacgca
     1561 tccgctccga ctcataggcc cgcatctcgg agtgaatgcg cagttcgcgc cgcctgagga
     1621 tgagctcgtc gcgctgggcg tagtaggcct gaaggcgctc ctcgtcgagt ttgcgctgct
     1681 gttcttcctc gcgggccagc cgctcctgct cgcgctgcag ccgttcgagt tcgtcgatgc
     1741 cctgctgcat gatggtgacg ccgaggcggg tggagagttc ggccatgtcc tcgctccgca
     1801 ccaacgcatc gggcacgccg gcataggcga tcgaaaggcc cggcagcccg gcacggcccg
     1861 ggaaggctac ggcgatctcc gcgtcgatgg cattgagcgc cagttcgccc accaccttca
     1921 cctcgacctt ggcctgcggc gtggtggcga cgaagggcgc gaaacggacc gcgccatcgg
     1981 aaatggtcac cggtccggcc acataaccga agccgagcgc ctggcccttg cgcagcaggt
     2041 cgaaggcggc ggtgaggccg ttgccgtcgc gcgccgcatc gacggc
//
LOCUS       DBNGVR010000164         2474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1042485, whole genome shotgun sequence.
ACCESSION   DBNGVR010000164 DBNGVR010000000
VERSION     DBNGVR010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1042485"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16A0_03530"
     CDS             <1..352
                     /locus_tag="AC16A0_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104431.1"
                     /translation="ARKVLDDLYAAQVGEMKALYVKREAEGRATADVAQAARAATFGA
                     IDTLLFDMDASEAGTISAEDGSVTFGKPGPGTYGIVDEIAGRALATGAKVMAVRKPDL
                     PAGDAVAAILRYPI"
     gene            complement(436..1323)
                     /locus_tag="AC16A0_03535"
     CDS             complement(436..1323)
                     /locus_tag="AC16A0_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104432.1"
                     /translation="MPSSKPLWLILAPVIFLLLWSGGFSVAKLGLKHVEPMSFLSLRY
                     ASVLVILLPLYFILRPPLPKRGMEWVHLLIVGFLIQAVYFGLSYIAFAAGVSAGGVAI
                     IVCLQPILVGLIAPRFAGEKVSALRWIGLALGLAGAATVVAARSAIAAESLAGIVAAV
                     GALLGITAGVLWEKRFGLSFHPVVSNSVQYVAGLAGTLPLALATEHFRFEWSNPEFLA
                     AWAYLVIGNSIIAMTLLLAMIRAGEVSRVSSLFYLVPPISALIAWPLLGEAMPPLGWA
                     GFALAALGVALASRKPQKG"
     gene            complement(1378..1677)
                     /locus_tag="AC16A0_03540"
     CDS             complement(1378..1677)
                     /locus_tag="AC16A0_03540"
                     /inference="COORDINATES: protein motif:HMM:NF025742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HIR3104433.1"
                     /translation="MRISATDLGKLPPHKDGWSRFDAMRDDEIAQRAASDPDSVPVDF
                     DWSGAELRFPQLKQAMSLRIDPDVLAFFKASGKGYQTRMNAVLRAYMEHRLRERR"
     gene            complement(1658..1978)
                     /locus_tag="AC16A0_03545"
     CDS             complement(1658..1978)
                     /locus_tag="AC16A0_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HIR3104434.1"
                     /translation="MGRTTMPMDWELTVLPVSNVEWNHEKAERNIKKHGVGFEDAARI
                     FLNPIVARDDDHGEEFRTVAIGLSGDRVLVVVFTERRDAVRIISARKATAAERKRLGA
                     HLRH"
     gene            2132..>2474
                     /locus_tag="AC16A0_03550"
     CDS             2132..>2474
                     /locus_tag="AC16A0_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR3104435.1"
                     /translation="MRFPPSFLDDIRSRVAISSVVGRKVQWDRRKTNAGKGDYWACCP
                     FHGEKSPSFHVEDKKGRYHCFGCKASGDIFTFLVEKEGLSFPEAVERLAMEAGLAMPK
                     VTEADARREEER"
BASE COUNT          464 a          804 c          814 g          392 t
ORIGIN      
        1 cgcgcgcaag gtgctcgacg atctctatgc cgcgcaggtg ggcgagatga aggcgctcta
       61 cgtgaagcgc gaggccgagg gccgcgccac cgccgacgtg gcgcaggccg cgcgcgccgc
      121 caccttcgga gccatcgaca cgctgctctt cgacatggac gccagcgagg ccggcaccat
      181 cagcgcggaa gatggcagcg tcaccttcgg aaagccgggt cctggcacct atggcatcgt
      241 cgacgagatc gccggacgcg ccctcgcaac gggtgcaaag gtcatggcgg tacgcaagcc
      301 cgatctcccg gcgggcgatg ccgtcgcggc catcctgcgc tacccgatct gaactctcca
      361 gcgcatcacc cttgccccgt caccccggcg aaagccgggg cccacggttg ggcctgatcg
      421 ggcaagcgaa cttcctcaac ccttctgcgg cttgcggctg gccagcgcca cgcccagggc
      481 cgccagcgcg aagcccgccc agcccagcgg cggcatggct tcgcccagca ggggccaggc
      541 gatcagcgcc gagatcggtg gcacgagata gaagagcgag gagacgcggc ttacctcgcc
      601 ggcgcggatc atggcgagca gcagggtcat ggcgatgatc gaattgccga tcaccagata
      661 ggcccaggcg gcaaggaatt ccgggttgct ccactcgaag cggaagtgct cggtggccag
      721 cgccagcggc agcgtgccgg cgagtcccgc cacatattgc acgctgttcg acaccacggg
      781 gtgaaacgag aggccgaagc gcttctccca caagacgccg gcggtgatgc cgagcagcgc
      841 gcccactgcg gcaacgatgc cggccaggct ttccgccgcg atggccgaac gcgccgcgac
      901 cacggttgcg gcacccgcaa gtccgagcgc cagcccgatc cagcgcagcg cgctcacctt
      961 ctcgccagcg aagcgcggcg cgatcagccc gacgagaatg ggctgcaggc agacgatgat
     1021 ggcaacgccg cccgccgaga cgcccgccgc gaaggcgatg tagctcagcc caaaatagac
     1081 agcctggatg aggaagccca cgatcagcag atgcacccac tccatgccgc gcttgggcag
     1141 cggcggccgc aggatgaaat agagcggcag caggatcacc aataccgagg catagcgcag
     1201 cgagagaaag ctcatcggct cgacatgctt gaggccaagc ttcgccaccg agaacccgcc
     1261 cgaccacagg agcaggaaga tgacgggcgc gaggatgagc cagagcggct tggaggaggg
     1321 catggcgagc agttagcccg gtccgcggct gcagcggaac tgcgttcttg gaatgcctca
     1381 gcgcctttcg cgcaaccggt gttccatgta ggcgcgcagc acggcattca tgcgggtctg
     1441 atagcccttg cccgaggcct tgaagaaggc caggacgtcg ggatcgatcc tcaggctcat
     1501 ggcctgcttg agctgcggaa agcgaagttc ggcacctgac cagtcgaagt ccaccggcac
     1561 gctgtcgggg tcggaggccg ccctttgggc gatctcgtcg tcgcgcatcg catcgaagcg
     1621 ggaccagcca tccttgtgcg gcggaagctt cccgagatca gtggcggaga tgcgcaccaa
     1681 gccgcttgcg ttcggccgcg gtcgcctttc gcgctgagat gattcggact gcgtctcgtc
     1741 gttcggtgaa gacgaccacg aggacgcggt ctcccgaaag cccgatcgcg acggtgcgga
     1801 attcttcccc gtggtcgtcg tcgcgagcga cgatcggatt gaggaagatc cgggcggcgt
     1861 cctcgaagcc gacgccgtgt ttcttgatgt tccgttccgc tttttcatgg ttccattcga
     1921 cattcgatac gggaagaacg gtcagttccc agtccatcgg catggtcgtg cggcccaaga
     1981 cttcgtctct tcgatgcgaa ggacgtttgg ccattcaagt tccgttgccg catccggttt
     2041 ccccattttc cggcaatgta tatacaattg tggtacggct gtcgcaacac atgtcttggg
     2101 gcgcggggca agagcgtcta gactcgccgc catgcgtttc ccgccctcat tccttgacga
     2161 catccgctcc cgggtcgcga tctcctcggt ggtcgggcgc aaggtgcagt gggaccggcg
     2221 caagaccaat gcgggcaagg gcgactactg ggcctgctgc cccttccatg gcgagaagtc
     2281 gccgtccttc cacgtcgagg acaagaaggg ccgctatcac tgcttcggct gcaaggcctc
     2341 gggcgatatc ttcaccttcc tggtcgagaa ggagggcttg agcttccccg aagcggtgga
     2401 acgcctcgcc atggaagccg ggcttgccat gcccaaggtc acggaagctg atgcaagacg
     2461 cgaggaagag cgcg
//
LOCUS       DBNGVR010000165         5720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3203547, whole genome shotgun sequence.
ACCESSION   DBNGVR010000165 DBNGVR010000000
VERSION     DBNGVR010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3203547"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..865)
                     /locus_tag="AC16A0_03555"
     CDS             complement(95..865)
                     /locus_tag="AC16A0_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR3104436.1"
                     /translation="MTRPSPRRGEILRLARERGTLAVSELASVLAVSEETIRRDVRPL
                     VAAGEVVKLHGAIALPHDVGEAPFERRMRTNADAKRAIAMHAARMIADGDSLMLDTGT
                     TTSILARELLRKRNLTVVTNSSDIARTLATVNGNRVYMAGGELNGDNGAAFGASAVQF
                     VSTFRVRHAFISIAAISAEAGPSDDYLAEAEFARMVLSCGERRYLLSDASKFGRRALI
                     RVCSFGEINVLITDQAPPASIASVLQDAGVTIEIAQRA"
     gene            1064..2035
                     /locus_tag="AC16A0_03560"
     CDS             1064..2035
                     /locus_tag="AC16A0_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104437.1"
                     /translation="MTRQSRWKSAVLASAALAAMPFLTPAPAGAAESSDPIKIALFDW
                     TSVNINARILGGIFEKLGYTVEYPTADYLSSLTTGLTNGDITVGVEFWDTTAGEAMAA
                     SEATGQTERLGALGPKAKEEWWYPLYMKEKCPGLPNWEALKDPKCAEAFSTPETAPNG
                     RYVGGPVTWEGFDDERVQALGLPFTVIHAGTDAAMFAELDSAYQRKAPVMVWVYSPHW
                     APAKYEGEWVEFPAHTKECYEDPKWGSNPDMAYDCGKPSGDIWKFAWGGMKDKWPIAH
                     KVAKAYQIDVNELNKLSGEVDLDGKSIEEVAAKWVADNEAKWKAWAQ"
     gene            2056..3117
                     /locus_tag="AC16A0_03565"
     CDS             2056..3117
                     /locus_tag="AC16A0_03565"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR3104438.1"
                     /translation="MKTKAAGADDPRPVKLSCHGLWKIFGSHAADFLRNHGGKPSAAD
                     LDRAHLIGAVRDATLEVREGEIFIVMGLSGSGKSTLVRCLSRLIEPTGGELIFDGKDL
                     LKASDSELIDIRRHKMGMVFQHFALLPHLNVLENVAFPLFIQGIGKTEREDRARQVIE
                     LVSLKGRERHYPSELSGGQQQRVGIARSLAVEPEIWFLDEPFSALDPLIRREMQTELM
                     RLQSVLKKTIVFITHDFDEAIRLADRIAIMKDGSIIQIGTPEELVRSPATDYVAEFTR
                     DVNRAKVLSARSLMTKATARAKGYDKIEASAKVASFAAAIVDRNAPLAVVDSMGAIIG
                     EVAPRSVIDLLSGKERGMA"
     gene            3114..5093
                     /locus_tag="AC16A0_03570"
     CDS             3114..5093
                     /locus_tag="AC16A0_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104439.1"
                     /translation="MSDATLPASVPRAAAPSLARWVVSGSVLAAVLIFILREQLPALS
                     SYPSELVIPFKEWIATAMTWLKVNFTWFTRSLTAIIDVPLRFAFNLLAKGFKFGSGEA
                     AWTLPRLSWLGIVLTMAYLGYIAGGRRLAALCGLSFLALAVFGVWDNAMLTLGLIIIC
                     VPFCVVTGLLIGIWAYRSPRVNQWLISPSLDLMQTIPTFAYLIPMLLLFGSNPVSALL
                     ATGLFATPPMVRATVLGLNKVPQEIKDFADMAGCTKRQKLWRVLVPSAKPSLMIGVNQ
                     VIMLALNMVIISSMIGAGGLGYDVLLALRALKIGQAMEAGLAIVLIAVVLDRLSQAFA
                     HRSPDAAPREGPWARHGNLLIVVGMLFVTTLAALAVPALDKLPEAMTITTAPYWKAGV
                     DWMTQNLFDTIEAVRVFLLLYFLNPLRTFFEALPWAGVLLILAALGWRIGGAGLAVLV
                     GLLGFFAVATGLWVPAMQTLYLAGASTLVACALGIPIGLWASRSDRVSAIVTPIIDTL
                     QTIPSFCFIIPVVMLFRVGDVTAMIATVAFAIVPAIRYTNHGIRQVPQQLIEAGQVSG
                     CTRWQLFRHVQLPVAMPEILLGINQTILLALSMIIICAMIGTRDLGQEIFKALAKADS
                     GRGLVAGLVIAFIGIIADRLITAWVHKTRARRGEA"
     gene            5090..>5720
                     /locus_tag="AC16A0_03575"
     CDS             5090..>5720
                     /locus_tag="AC16A0_03575"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HIR3104440.1"
                     /translation="MTPAEQRVRALPIWQGLKDLVPLKGGVSNASFTVTDATGRYVAR
                     VGEDYPFHQVSRDRETIASRAAYAAGLSPEVVHTAPGIMVVRYLDARTYAEADVRANA
                     AACVEIVKRCHREMGRRITGQGAIFWVFQILRDYAHTLTAGKHRHVPDLPRLMAILDD
                     LEAAQAPLPIVFGHHDLLPTNFLDDGHRLWLIDWEYGAFGTAMFDLANIA"
BASE COUNT          988 a         1848 c         1804 g         1080 t
ORIGIN      
        1 tcacctgttt tgggaatccc gcggcagttt ggtccatcgc cgttgcccgg tcaactcccg
       61 caggctgcgg ctgcatgcca ccgctgcgat gcgatcaagc ccgctgcgca atctcgatcg
      121 tgacgcccgc atcctgcaag acacttgcga ttgacgccgg gggcgcctgg tcggtgatca
      181 ggacattgat ttcgccgaaa ctgcagacgc ggatcagggc gcggcggccg aacttgctgg
      241 cgtcgctcag caggtaacgg cgttcgccgc aggacagtac catgcgcgcg aattccgctt
      301 ctgccagata gtcgtcggaa ggtccggcct cggcgctgat ggcggcaatg gagatgaagg
      361 catggcgtac gcggaaggtg gagacgaatt gtaccgccga agcgccgaag gccgcaccat
      421 tgtccccgtt cagttcgcca cccgccatgt agacccgatt gccattgacc gtggcaagcg
      481 tccgggcgat gtcgctggaa ttggtcacca ccgtgaggtt gcgcttgcgc aacagctcgc
      541 gcgcgagaat ggaggtggtc gttcccgtat cgagcatgag gctgtcgccg tcggcgatca
      601 tgcgtgccgc gtgcatggcg atggcgcgct tggcatccgc attggtccgc atacgccgct
      661 caaagggggc ttcgccgaca tcatggggca aggcaatggc gccgtgcagc tttacgacct
      721 ccccggcagc caccagcggc cggacatcgc gacggatggt ctcttccgag acggcgagca
      781 ccgatgccaa ctccgatacg gccagggtgc cgcgttcccg cgcaagcctc aggatctctc
      841 cgcgtcgcgg gctagggcgc gtcatgttgg aattgtgcct gtttcttagt cattttgccg
      901 cattctaatc agaaatgcgc ccaatgccac atcagattcg gtcaagccca caataatcaa
      961 catcggctgt gaccgccgca ttgacacggc tgtggctttc ggtcgagact gccggcggag
     1021 gcgctgcatc agacaagcgt ccgaaaaaat tgcgagggag caaatgacca gacagtctcg
     1081 ttggaaatct gccgtgcttg ccagcgccgc ccttgcggcg atgcccttcc tgacccccgc
     1141 gcctgccggc gccgcggagt cgtccgaccc gatcaagatc gccctgttcg actggaccag
     1201 cgtgaacatc aatgcgcgga ttcttggcgg cattttcgag aagctgggct acacggtcga
     1261 gtatccaacg gcggactatc tctcgtcgct gaccaccggg cttaccaacg gcgacattac
     1321 cgtcggcgtt gagttctggg ataccacagc cggcgaagcc atggccgctt ccgaggcgac
     1381 agggcagacc gagcggcttg gcgcacttgg tccgaaagcc aaggaagagt ggtggtatcc
     1441 gctctacatg aaggagaagt gccccggtct tccgaactgg gaagcgctga aggatccgaa
     1501 gtgcgcagaa gccttttcga cgcctgaaac cgcaccgaat ggccgctatg tcggcgggcc
     1561 tgtgacctgg gaaggtttcg acgacgaacg cgttcaggcc ctcggcctgc ccttcaccgt
     1621 cattcatgcc ggcacggatg ccgccatgtt cgccgaactc gactcggcct atcagcgcaa
     1681 ggcgcccgtc atggtgtggg tctattctcc gcattgggcg cccgccaagt atgaaggcga
     1741 gtgggtggaa ttcccggcgc acaccaagga gtgctacgaa gacccgaagt ggggcagcaa
     1801 tcccgacatg gcttacgact gcggcaagcc ctccggcgat atctggaagt ttgcctgggg
     1861 cggcatgaag gacaagtggc cgatcgccca caaggtggcc aaggcctacc agatcgatgt
     1921 caacgagctg aacaagcttt cgggcgaagt cgatctcgac ggcaagtcca tcgaggaagt
     1981 ggccgcgaaa tgggtcgccg acaacgaagc caagtggaag gcctgggccc agtaagaccg
     2041 gccggggagc ctggcatgaa aaccaaagcc gcgggcgcgg atgatccgcg cccggtcaag
     2101 ctgtcctgcc atgggctctg gaagatcttc ggttcgcatg cggccgattt tctccggaac
     2161 catggcggca agccaagtgc cgcggacctc gatcgcgcgc atctcatcgg tgcggtgcgc
     2221 gatgcgacgc ttgaagtccg cgaaggcgag atcttcatcg tcatgggttt gtccgggtcc
     2281 ggcaagtcca cgctggtgcg ctgcctctcg cgcctgatcg aacccactgg cggcgaactc
     2341 atcttcgatg gaaaggacct gctcaaggcc agcgactccg agctgatcga catccggcgc
     2401 cacaagatgg gcatggtgtt ccagcatttc gcgctcctgc cacacctcaa tgtgctcgag
     2461 aacgtggcct tcccgctgtt catccaaggc atcggcaaga ccgagcgcga ggaccgcgcg
     2521 cgccaggtga tcgaactggt gagcctcaag ggccgcgagc gccactaccc gtcggaactc
     2581 tcgggcggtc agcagcagcg ggtggggatc gcccgctcac tcgcggtcga acctgagatc
     2641 tggttcctcg atgagccatt ctccgcactt gatcccctga tccgccgcga gatgcagacg
     2701 gagctgatgc ggctgcagag cgtcctgaag aagaccatcg tcttcatcac gcatgacttc
     2761 gacgaagcca tccgcctggc cgaccgtatc gccatcatga aggacggatc gatcatccag
     2821 atcggcacgc ccgaagaact ggtgcgcagc cccgcgacgg actacgtggc agagttcacc
     2881 cgcgacgtga atcgcgccaa ggtgctgtcg gcccgcagcc tgatgaccaa ggcgacggcc
     2941 agggcgaagg gctatgacaa aatcgaagcc agcgccaagg tcgccagctt cgccgcggcc
     3001 atcgttgatc gcaatgcacc gctggcggtt gtcgactcga tgggcgcaat catcggcgaa
     3061 gttgcgccgc gatcggtgat cgatctgctg tcaggcaagg aacgggggat ggcatgagcg
     3121 acgcgacgct gccggcttcg gttccccggg ccgccgcgcc gtctctggcg cgctgggttg
     3181 tatccggctc cgtccttgcc gcagtcctga tcttcatcct gcgtgaacaa ctgccggcgt
     3241 tgagcagcta tcccagcgaa ctggtgattc ccttcaagga gtggattgcg acggcgatga
     3301 cctggctgaa ggtgaacttc acctggttca cccgttccct cacggccatc atcgacgtgc
     3361 cgctgcgctt cgccttcaac ctcctggcca agggcttcaa gttcggcagt ggcgaagcgg
     3421 cctggacgct gccgcgcctg tcgtggctgg gcatcgtgct caccatggcc tatctgggat
     3481 acatcgccgg cggccgcagg ctggcggccc tgtgcggatt gagcttcctg gcgctggcgg
     3541 tcttcggcgt gtgggacaat gccatgctta cgctggggct gatcatcatt tgcgtcccct
     3601 tctgcgtggt gaccggcctg ctgatcggca tctgggccta ccgcagcccg cgggtgaacc
     3661 agtggctgat ctcgccgtcg ctggacctca tgcagaccat accgaccttc gcctacctca
     3721 ttccaatgct gttgctgttc gggtccaatc cggtgtcggc acttcttgcc acggggcttt
     3781 ttgcaacgcc gcccatggtg cgcgccacgg tgctgggcct caacaaggtg ccccaggaga
     3841 tcaaggattt tgccgacatg gccggctgca cgaagcggca gaaactgtgg cgcgtgctgg
     3901 tgccctcagc caagccatcg ctgatgatcg gcgtgaatca ggtcatcatg ctggcgctca
     3961 acatggtgat catctcctcg atgatcgggg ccggcggcct tggctacgat gtgctgctgg
     4021 ccttgcgcgc cctcaagatc ggtcaggcga tggaggcggg tctcgccatc gtgctgatcg
     4081 ccgtcgtgct ggaccgcctg agccaggcct tcgcccatcg cagccccgac gcggccccgc
     4141 gggaaggccc gtgggcgcgc cacggcaatc ttctgatcgt tgtcgggatg ctcttcgtca
     4201 ccacgcttgc agcgcttgcc gttcccgcgc tcgacaagct gccggaagcg atgaccatca
     4261 ccaccgcgcc ctactggaag gccggcgtcg actggatgac gcagaacctc ttcgatacca
     4321 tcgaggccgt tcgcgttttc ctgcttctgt acttcctcaa tccgctgcgc acgttcttcg
     4381 aggccctgcc gtgggccggc gtgctgctga tccttgcggc actgggctgg cggatcggcg
     4441 gggccggcct ggccgttctg gtaggtcttc tcggcttctt cgcggtggcg acgggtctct
     4501 gggttcccgc catgcagacc ctttatctgg ccggtgcctc cacgctggtg gcctgcgcgc
     4561 ttggcattcc tatcggcctc tgggcgtcgc gcagcgatcg cgtctccgcc atcgtgacgc
     4621 cgatcatcga cacgctacag accatcccgt ccttctgctt catcatcccc gttgtcatgc
     4681 tcttccgcgt cggtgacgtc accgccatga tcgccaccgt ggccttcgcc atcgtgcccg
     4741 ccatccgcta caccaaccat ggcatccgcc aggtgccgca gcagctgatc gaggccgggc
     4801 aagtgtcggg gtgcacgcga tggcagctgt ttcgccacgt gcaactgccc gtggcgatgc
     4861 ccgagatcct gctcggcatc aatcagacca tcctgctggc actcagcatg atcatcatct
     4921 gcgccatgat cggcacgcgc gacctgggcc aggagatctt caaggcgctg gcaaaggcgg
     4981 attccgggcg tggcctcgtc gccggtctcg tcatcgcctt catcggaatc attgccgacc
     5041 ggctgatcac ggcctgggta cacaagacac gggcgcgacg gggcgaagca tgacgccggc
     5101 cgaacagcgt gtgcgggcgc ttccgatctg gcagggtctg aaggatctgg tgccgctcaa
     5161 gggcggggtc agcaatgcca gctttaccgt gaccgacgcg accgggcgct acgtggcgcg
     5221 ggtgggggaa gactatccgt tccaccaggt ctcccgcgac cgcgagacca tcgcatcccg
     5281 tgcagcctac gcggccgggc tttcgcccga agtggtccat actgcgccgg gcatcatggt
     5341 cgtgcgctat ctggacgcga ggacctatgc ggaggccgat gtccgcgcca atgcggcggc
     5401 ctgcgtcgag atcgtcaagc gctgccatcg cgagatggga cggcgcatca cggggcaggg
     5461 cgccatcttc tgggtgttcc agatcctgcg cgactacgcg cataccctga cggccggcaa
     5521 gcaccgccat gtgcccgacc tgccgcggct catggccatc ctggacgatc tggaggcggc
     5581 gcaggcaccg ctgccgatcg tgttcggtca tcatgacctg ctgcccacca atttcctgga
     5641 cgacggacac cggctctggc tgatcgactg ggagtatggc gccttcggca ccgccatgtt
     5701 cgacctcgcc aacatcgcgt
//
LOCUS       DBNGVR010000166         5357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1828939, whole genome shotgun sequence.
ACCESSION   DBNGVR010000166 DBNGVR010000000
VERSION     DBNGVR010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1828939"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="AC16A0_03580"
     CDS             complement(<1..486)
                     /locus_tag="AC16A0_03580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HIR3104441.1"
                     /translation="MSRPGSGKKPATRSLKQRVKTGKGRTVSQKIWLERQLNDPYVKE
                     ARRLGYRSRAAFKLIEIDDKYKFLKPGGRVVDLGAAPGGWSQVAAQRVKAEQGKGRVV
                     AIDMHGMDPIPAVTIFKKDFYDEDAPQTLIDALGGEKADCVLSDMATHATGHRQTDHI
                     NI"
     gene            complement(483..1553)
                     /locus_tag="AC16A0_03585"
     CDS             complement(483..1553)
                     /locus_tag="AC16A0_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3104442.1"
                     /translation="MDATHGAGQARSRRKAGPHSGSNHSKDLRTHRPEALYGALDLGT
                     NNCRLLIATPADQGFVVVDAFSRIVRLGEQLGQTGRLSDAAMSRTIDALRVCANKLKW
                     RKVTKSRLIATEACRMAGNGADFIARVEAETGLALEIIDRETEARLAAVGAEPLVEAE
                     AETALVFDIGGGSTEVLWLQRKGQRFEIAAWTSLAAGVVTISERFGGGMDVTPESFEA
                     MCAYLRPMLQDFVRQVEAVTGRQPVPSHLLGTSGTVTTIAGVQLGLPRYDRSRVDGCW
                     LNSADIGKVTGDLLSFTYEQRAANPCIGRERADLVLAGCAILEEIRLAFPAERIRVAD
                     RGLREGILTQLMKQDGTYGRPQ"
     gene            1755..2612
                     /locus_tag="AC16A0_03590"
     CDS             1755..2612
                     /locus_tag="AC16A0_03590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3104443.1"
                     /translation="MRTFGQKWFKQDSLIGEFSEHLGVAVTQKKQAVALRAAKVDAEL
                     ASKAKSEFIANMSHELRTPLNAIIGFSDMLHTQTVSSPEKVQQYTGYIKQAAEHLLAL
                     INGILDVSKIQAGKLSVDREQVEMSSILSSCLLIIDAKAREKSITLETAVSPDMPRIM
                     GDPLRIKQVLINLVGNAVKFTPEGGRIKVDVRPLQPGYAVIEVTDTGCGMTPAEVETA
                     MRPFGQIDTAFSKRHEGTGLGLPIAYALARLHGGDLRIDSQKGQGTRVTVILPTVELP
                     TDAEQQNPN"
     gene            2701..4545
                     /locus_tag="AC16A0_03595"
     CDS             2701..4545
                     /locus_tag="AC16A0_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3104444.1"
                     /translation="MAVTGAHAVILLDADDSTLAVQMKGPEIGTLLKVDTPKAISLCM
                     ISALSSPMPSHARDENEMRIVEVEFIGELPKDGSGQPKSFRRGISCYPSLGDTVFRAS
                     KSELEKAYACDAETAVRVGNIQQDSTIPAMIKIDELLGKHFAVLGTTGTGKSCTVALI
                     LRRILEKNPQAHILLLDVHREYANAFRDYAEVISPDNMNLPFWLLNFDEIVEILIGQQ
                     PNREADIEVLRELIPLAKVRYVNNQRRDRAASGRGMMESTNVGVDTPIPYRTSDLTGI
                     LEEYIGELELRGELAPYKRLKARLETISRDPRYAFMFGSLTVQDTMAQVLSRLFRIPV
                     AGKPIAILELGGLPGEIINVVVSVLARLAFDFGVWSAGRIPITFVCEEAHRYVPIDRT
                     LGFEPTKRAISRIAKEGRKYGVSLCIVTQRPAELDPTILSQCNTIFSMRLTNERDQEI
                     LRAGISDAAASLLEFMPTMGTGEAITFGEGVALPTRIKFDMLPADSWPKSNTASFTKN
                     WAMDMPDDTFLHEIVMRWRAQSHNPDAMSYVAEHQAPAAPPAAASGPSTPSLQSSQPS
                     LRRTMPQGGGGLAQAAAPRPQAPMPQAAPASPGGQQQSLASLIRQFRS"
     gene            complement(4542..>5357)
                     /locus_tag="AC16A0_03600"
     CDS             complement(4542..>5357)
                     /locus_tag="AC16A0_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3104445.1"
                     /translation="FGRTDLRVHRKIVVIDGAAAWTGSMNMVDPRFFKTDAGVGEWVD
                     AMARIEGPAASLLAAVMIGDWVAEGGESVHSVIAAARLEGAAARGGVPVQVAASGPGE
                     SFDGLLQVMLALVNAARREIVMTTPYFVPDDALVLALRGALARGVRVVVIQPEKIDSL
                     LVRYASRSFFDDITAMGGEVHFYRSGLLHTKSITIDGEISMFGTANLDMRSLWLNYEV
                     SLLVYDEDFATRLRALQQGYIKRSLPLDLTSWSARPFHERLAESALRLMSPLL"
BASE COUNT          971 a         1861 c         1600 g          925 t
ORIGIN      
        1 gatgttgatg tggtcggtct ggcggtggcc cgtggcatgt gttgccatgt ccgacagcac
       61 gcagtcggcc ttctcgccgc ccagcgcatc gatgagagtc tggggcgcgt cctcgtcgta
      121 gaagtccttc ttgaagatgg tcacggcggg tatcgggtcc atgccatgca tgtcgatggc
      181 caccaccctg cccttgccct gttccgcctt gacccgctgg gccgccacct ggctccagcc
      241 tccgggtgcg gcgcccaggt cgaccacccg gccgcccggc ttcaggaact tgtacttgtc
      301 gtcgatctcg atgagcttga aggcggcgcg cgagcgatag ccgagacggc gcgcctcctt
      361 cacgtaggga tcgttgagct ggcgctccag ccagatcttc tgcgacacgg tgcgtccctt
      421 gccggtcttc acccgctgct tgagactgcg cgtcgccggt ttcttgccgc tgccgggcct
      481 gctcattgcg gcctcccgta ggttccgtcc tgcttcatga gctgcgtgag gatgccctcg
      541 cgcagcccgc gatcggccac gcggatccgc tcggccggaa aggccaggcg gatttcctcg
      601 agaatggcgc aacccgccag cacgagatcg gcccgttccc gcccgatgca gggattggcg
      661 gcgcgctgtt cgtaagtgaa ggacaaaagg tcgccggtca ccttgccgat gtccgcactg
      721 ttgagccagc agccatccac ccgcgaacgg tcatagcgcg gcagccccag ttgcacgccc
      781 gcaatggtgg tcaccgtgcc cgatgttccc agcagatggc tgggcaccgg ctggcggccg
      841 gtgacggcct caacctgccg cacgaagtcc tgcagcatcg gccgcagata cgcgcacatg
      901 gcctcgaagg actcgggcgt cacgtccatg ccgccgccga agcgttccga gatggtcacc
      961 acgcccgctg ccagcgatgt ccaggccgcg atctcgaagc gctggccttt gcgctgcagc
     1021 cacaggacct ccgtcgaacc gcccccgatg tcgaagacca gcgccgtctc ggcttccgcc
     1081 tcgaccagcg gttcggcacc gacggcggcg agacgcgctt cggtttcccg gtcgatgatc
     1141 tcgagtgcga gcccggtttc ggcctccacg cgcgcgatga agtcagcacc gttgccggcc
     1201 atgcggcagg cctcggttgc gatcagccgc gacttggtga ccttccgcca cttgagcttg
     1261 ttggcgcaga ccctgagggc atcgatggtg cgcgacatgg ccgcatccga caggcggccg
     1321 gtctgtccaa gctgctcgcc aagacggacg atgcgcgaga aggcatcgac cacgacgaag
     1381 ccctgatcgg ccggggtggc gatcagcaac ctgcaattgt tggttccgag atccagcgca
     1441 ccatagagag cttccggacg atgagtccgc aagtccttgg aatggttgga cccgctatgc
     1501 gggcccgcct tgcgccggga ccgggcctgg cccgccccat gggtcgcgtc cacgcgttct
     1561 aacctttcgg gacgggtgcg ctcgacaacc gcagctggag cgccagcccc cgtccataat
     1621 catttcgtta gcatcaatgt agacatcttc gtcttgggta acaattcaaa agtcagaccc
     1681 gacaaatgct gaaccccgcg ttaaccgcgc attgagacgt tgccgcctaa atagtcgtcg
     1741 gaactggggt tttcatgcgg acttttggcc aaaaatggtt caagcaggac tcgctgatcg
     1801 gcgagttttc ggagcacctg ggtgtcgcgg tcacgcagaa gaagcaggcc gttgcgctcc
     1861 gcgccgccaa ggtcgacgcc gagctcgcct ccaaggccaa gtcggaattc atcgccaaca
     1921 tgagccatga gctgcgcacg ccgctcaacg ccatcatcgg cttctccgac atgctgcata
     1981 cccagacggt gagttcgccc gagaaggttc agcagtatac ggggtatatc aagcaggcgg
     2041 ccgagcatct cctggcgctg atcaatggca tcctcgacgt ttcgaagatc caggcgggca
     2101 agctcagcgt cgaccgcgag caggtcgaga tgtcctccat cctgtcctcc tgcctgctca
     2161 tcatcgacgc aaaggccagg gagaaatcga ttactctgga aacggcggtc tcccccgaca
     2221 tgccgcgcat catgggcgat ccgctgcgca tcaagcaggt gctgatcaac ctggtgggca
     2281 atgccgtgaa attcacgccg gagggcggtc gcatcaaggt tgatgtgcgc ccgctgcagc
     2341 ccggctatgc ggttatcgag gtgaccgaca ctggatgcgg catgacgccg gccgaagtcg
     2401 agactgccat gcggcccttc ggccagatcg acaccgcctt cagcaaacgc cacgaaggca
     2461 ccggcctggg cctgcccatc gcctatgcgc tggcacggct gcatggcggc gacctccgca
     2521 tcgattcaca gaagggccag gggacgcggg tcaccgtgat cctgccgacc gtagaacttc
     2581 cgaccgacgc cgaacaacaa aaccccaact gagacgcagg gcattatgta tcgtcagcag
     2641 gtttccccga cttccctgaa ctttccccgc gacgactcga tcgagcgcgt cggcaggatc
     2701 gtggctgtta ccggcgccca cgctgtcatt cttctcgatg cagacgactc caccctcgcg
     2761 gtacagatga agggcccgga gatcggaaca ctgctcaagg tcgatacgcc caaggcgatt
     2821 tcgctttgca tgatttccgc cctgagttcg cccatgccgt cgcatgcccg cgacgagaac
     2881 gagatgcgga tcgtcgaggt cgagttcatc ggcgagcttc ccaaggacgg cagcggccag
     2941 ccgaagtcgt tccgccgcgg catttcctgc tacccctcgc tgggcgacac ggtgttccgc
     3001 gccagcaagt cggaactcga gaaggcctat gcctgcgatg ccgagaccgc cgtgcgcgtc
     3061 ggcaacatcc agcaggactc gaccatcccg gcgatgatca agatcgacga gctgctgggc
     3121 aagcatttcg ccgttctcgg taccaccggc accggcaagt cctgcaccgt ggccctgatc
     3181 cttcgccgca ttctcgagaa gaacccgcag gcccatatcc tgcttctcga cgtgcaccgc
     3241 gaatatgcca atgccttccg cgactatgcc gaagtcatct cgcccgacaa catgaacctg
     3301 cccttctggc tgctcaactt cgacgagatc gtcgagatcc tgatcggtca gcagcccaat
     3361 cgcgaggccg acatcgaagt gcttcgcgag ctcataccgc tggccaaggt ccgctacgtc
     3421 aacaaccagc gccgcgatcg cgcggccagc ggccgcggca tgatggaatc caccaatgtc
     3481 ggcgtcgata cgccgatccc ctatcgcacc tctgatctca ccggcatcct cgaggaatac
     3541 atcggcgagc tcgaactgcg cggcgaactg gcgccctaca agcgcctcaa ggcccggctc
     3601 gagaccatca gccgcgatcc acgctacgcc ttcatgttcg gcagcctgac ggtgcaggac
     3661 accatggccc aggtgctgtc gcgcctgttc cgcatcccgg ttgcgggaaa gcccatcgcc
     3721 atcctcgaac tgggcggcct gccgggcgag atcatcaatg tcgtggtctc ggtgctggcc
     3781 cgcctcgcct tcgacttcgg ggtatggagc gccggccgca ttcccatcac cttcgtttgc
     3841 gaagaggccc accgctacgt gccgatcgac cgcacgctgg gcttcgagcc gaccaagcgg
     3901 gccatctcgc ggatcgccaa ggaaggccgc aaatacggcg tctcgctgtg cattgtgacg
     3961 cagcgcccgg ccgaactcga cccgacaatc ctctcccagt gcaacaccat cttctcgatg
     4021 cgcctcacca atgagcgcga ccaggaaatc ctgcgcgcag gtatctccga cgctgccgcc
     4081 agcctgctcg aattcatgcc gaccatgggc accggcgagg ccatcacctt cggcgaaggc
     4141 gtggcgctcc ccacccgcat caagttcgac atgctgccgg ccgattcctg gcccaagagc
     4201 aacaccgcct ccttcaccaa gaactgggcg atggacatgc cggacgacac cttcctgcat
     4261 gagatcgtga tgcgctggcg cgcccagagc cacaatcccg acgcgatgtc ctacgtggcc
     4321 gaacatcagg cgccggctgc gccgccggct gcggcctcgg gcccctccac gccatcgctg
     4381 caatccagcc agccgagcct gcgccggacc atgccccagg gcggcggcgg tcttgcccag
     4441 gccgctgcgc cgcggccgca ggcgcctatg ccgcaggccg cacccgcctc gccgggcggc
     4501 cagcagcaaa gccttgccag cctgatccgg cagttccgca gctagagcag cggactcatc
     4561 agacgcagcg cgctttccgc caggcgctcg tggaagggcc gtgccgacca gcttgtcagg
     4621 tcgagcggca gggaccgctt gatgtagccc tgctgcaacg ccctgagccg cgtggcgaag
     4681 tcttcatcgt agaccagcaa cgaaacctca tagttcagcc acaggctccg catgtcgaga
     4741 ttggcggtgc cgaacatcga aatctcgccg tcgattgtga tcgacttcgt gtgcagcagg
     4801 ccgctccggt agaaatgcac ttccccgccc attgccgtaa tgtcgtcgaa aaacgaccgg
     4861 ctggcatagc gcaccagcag ggagtcgatc ttctccggct ggatcacgac cacgcgaacg
     4921 ccgcgcgcca atgcgccgcg caaggccagc accagtgcat cgtcaggcac gaagtagggc
     4981 gtcgtcatca cgatttcgcg gcgggccgcg ttgaccagcg ccagcatcac ctgcagcagc
     5041 ccgtcgaagg attcgcccgg gcccgatgcc gcaacctgaa ccggaacgcc gccgcgcgca
     5101 gcagcgccct caagccttgc cgcagcaatc acactgtgaa cgctttcacc gccctcggcc
     5161 acccagtcgc cgatcatcac ggctgccagc aggcttgccg ccggtccttc gatgcgggcc
     5221 atggcgtcga cccattcacc gaccccggca tccgtcttga agaagcgcgg gtccaccatg
     5281 ttcatgctgc cggtccaggc ggcggcaccg tcgatgacga cgatcttgcg gtggacccgg
     5341 aggtcggtgc gcccaaa
//
LOCUS       DBNGVR010000167         2636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4971669, whole genome shotgun sequence.
ACCESSION   DBNGVR010000167 DBNGVR010000000
VERSION     DBNGVR010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4971669"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="AC16A0_03605"
     CDS             complement(<1..1001)
                     /locus_tag="AC16A0_03605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3104446.1"
                     /translation="MSSPGATQPRYGNLVAAIATVAACDIAMGLTLQLLPLLMEAGGH
                     PAWIIGANTAMGPIGILLAGPLLPRLIGNIGSRRIVYFVAIILIVALLAMKLAPSIWW
                     WFPLRLIFGIAAGTLFTVSEAWVLTFSSDGNRGRIMGLYTSVLAVTFSVGPLIIPWTG
                     IDGWTPWLIAIVCILVALLPLAFVKVSDDAFKQEKHGSFFRFVRRAPLLLFAVCAATL
                     FDNVFISFFTIFGLRNGVELDVASRILGFGIIGNVVLFYPMGWLADHWSRRGVVILTA
                     CSTIVLSLSLIWLINTPFIWITVVLLSASAFGVYVVALTPMGDTFKGPDVVAGSAAFA
                     "
     gene            1115..1756
                     /locus_tag="AC16A0_03610"
     CDS             1115..1756
                     /locus_tag="AC16A0_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018446669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3104447.1"
                     /translation="MTQLQAFLIPVTGFQQNCSLVFDKDQKRGAIVDPGGDIPLILDA
                     IAQSGVTIEKILLTHGHVDHAGGAAELRDTLKVPIIGPHEDDLFLLEELAGTGAQYGI
                     MGARNVKPDRWLKDGDKVSVAGLDFDILHAPGHSPGSVVFFNDANRFALMGDVLFQGS
                     VGRTDLPRGSHETLLRSIRKKILPLGDDVVFLPGHGPASTIGQERQTNPFLNE"
     gene            1802..>2636
                     /locus_tag="AC16A0_03615"
     CDS             1802..>2636
                     /locus_tag="AC16A0_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3104448.1"
                     /translation="MSKHVPPPENPKDIEPIGLKEALEERYLAYALSTIMHRALPDVR
                     DGLKPVHRRLLHVMRQLRLDPNTAFKKSAKIVGDVMGGYHPHGDQSIYDALVRLAQDF
                     ASRYPLVDGQGNFGNIDGDSAAAYRYTEARMSEVARLLLDGLDEDTVDFRPNYDGSLQ
                     EPVVLPGAFPNLLANGSTGIAVGMATSIPPHNAAELCDAALHLIKAPNATVEKLAQLV
                     PGPDFPTGGIVIDDRASIIEAYRSGRGSFRVRAKWSQEDLGRGAWQIVVTEIPYLVQK
                     GR"
BASE COUNT          518 a          811 c          859 g          448 t
ORIGIN      
        1 gcgaaggccg ccgatcccgc caccacgtcc ggtcctttga aggtgtcgcc catgggtgtg
       61 agtgccacga catagacacc gaaggccgag gcggagagca acaccaccgt gatccatatg
      121 aatggcgtgt tgatgagcca gatcaggctc agagacagaa cgatggtgct gcaggcggtg
      181 aggatgacga cgccgcgccg cgaccagtga tcggccagcc agcccatggg atagaacagc
      241 accacgttgc cgatgattcc gaagccgagg atgcggctcg ccacatcgag ttccacgcca
      301 ttgcggaggc cgaagatggt gaaaaacgag atgaagacat tgtcgaagag cgtcgcggcg
      361 cagacggcga agagcagcag cggcgcccgc cgcacgaagc ggaagaagct accgtgcttc
      421 tcctgcttga acgcgtcgtc actgaccttg acgaaggcga gcggcagcag ggcgacgagg
      481 atgcagacga tggcgatcag ccacggcgtc cagccgtcaa tcccggtcca gggaatgatc
      541 agcggcccga ccgagaatgt gacggcgagc acgctggtgt agagtcccat gatgcggccg
      601 cggttgccgt cacttgaaaa ggtcagcacc caggcctcgc tgacggtgaa gagcgtgccg
      661 gcggcaatgc cgaagatcag gcgcagcggg aaccaccacc agatcgaggg cgcgagcttc
      721 atggcaagca gcgcgacgat caggatgatg gcaacgaaat agacgatgcg gcgtgacccg
      781 atgttgccga tcaggcgggg cagaagcggc ccggcgagca ggatgccgat cggccccatg
      841 gccgtgtttg cgccgatgat ccaggccgga tgaccgcctg cttccatgag caggggcagc
      901 agttgcagcg tgagtcccat ggcaatgtcg caagctgcca ccgtcgcgat cgcagcgacg
      961 aggttgccat agcgcggctg tgtagccccg ggggaagaca ttggatcggt tctcttgaat
     1021 cgtcgttcaa caggctgtag ctggccgccc ggcagggggc aagggcgggg gccggtgttg
     1081 cgcttgcccc tcagcgcagc tatgcagcgc cgccatgaca cagcttcaag ctttcctcat
     1141 tcccgtgacg gggtttcagc agaactgttc gcttgtcttc gacaaggacc agaagcgcgg
     1201 tgcgatcgtc gatcccggag gtgacatccc attgatcctc gacgccatcg cgcagtccgg
     1261 cgtgacgatc gagaagatcc tgctcacgca tggccatgtc gatcatgccg gcggtgccgc
     1321 cgaactccgc gacaccttga aggtgccgat cattgggccc catgaagacg acctgtttct
     1381 tctggaggag cttgctggaa cgggtgcgca gtatgggatt atgggtgcgc gcaatgttaa
     1441 gccggatcgc tggctgaagg acggcgacaa ggtcagcgtg gcaggactcg acttcgacat
     1501 cctgcatgcg ccgggccatt cgccaggatc ggtggtcttt ttcaatgatg ccaaccgctt
     1561 cgcactgatg ggcgacgttt tgttccaggg ttccgtaggc cgtaccgacc tgccgcgcgg
     1621 cagccacgag accctgttgc ggtcaatccg caagaagatc ctgccgttgg gcgacgatgt
     1681 cgtcttcctg cctggccacg gcccggccag caccatcggc caagagcgcc agaccaaccc
     1741 cttccttaac gaatagggcg ggtccgggcc ttcccggagc ccgtgatata atcgccgaat
     1801 catgagcaag cacgttccgc cgcccgagaa tccgaaagac atcgaaccca ttggcctgaa
     1861 ggaagcgctg gaagagcgct atctggctta tgcgctctcc accatcatgc accgcgctct
     1921 gccggatgtg cgcgacgggc tgaagccggt gcaccgccgg ctgctgcatg tgatgcggca
     1981 gctgcggctc gatcccaata cggcgttcaa gaagtcggcc aagatcgttg gcgacgtcat
     2041 gggcggctat catccgcatg gcgaccagtc gatttatgat gcactggtcc ggctcgcgca
     2101 ggatttcgcc tcgcgctatc cgctggtcga cgggcagggc aacttcggca atatcgacgg
     2161 cgacagcgcc gcggcctatc gctacactga ggcccgcatg tccgaggtgg cgcgcctgct
     2221 gctcgacggt ctggacgagg acacggtcga tttcaggccc aactacgacg gctcgctgca
     2281 ggagccggtg gtgctgccag gggcctttcc caatctcctt gccaacgggt cgactggcat
     2341 cgcggtgggc atggcaacct ccattccgcc gcacaacgcc gcggagctct gcgacgccgc
     2401 cctgcatctg atcaaggcgc ccaatgccac ggtcgagaag ctggcccagc tggtgcccgg
     2461 ccccgacttt ccgaccggcg gcatcgtcat tgatgaccgc gcctcaatca tagaagctta
     2521 ccggagcgga cgcgggtcct tccgggtccg cgcgaagtgg tcgcaggaag accttggccg
     2581 cggcgcgtgg cagatcgtcg tcacggagat tccctatctc gttcagaagg gccggc
//
LOCUS       DBNGVR010000168         2831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_454358, whole genome shotgun sequence.
ACCESSION   DBNGVR010000168 DBNGVR010000000
VERSION     DBNGVR010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_454358"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="AC16A0_03620"
     CDS             complement(<1..211)
                     /locus_tag="AC16A0_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104449.1"
                     /translation="MTPRSFVLLTVFAAVTLTLMPLQQLFVWFWPGMARRFPMHYHRM
                     ICGLLGIRLRVIGQPPRQGPVLIASN"
     gene            complement(208..1317)
                     /gene="tsaB"
                     /locus_tag="AC16A0_03625"
     CDS             complement(208..1317)
                     /gene="tsaB"
                     /locus_tag="AC16A0_03625"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3104450.1"
                     /translation="MKLLALDTSMAACSAAILDTDRDVILARRWVAMERGHAEALAPM
                     VAEVLQEAELHPRDLSRIAVTAGPGTFTGVRIGLAMARGLGLALGIPVAGLDSLTAIA
                     ANVPPGRPVLVAADARKEEVYAAGFDADGTRRFGPEVMNLGEAARRLAPGSVVLGTAA
                     ERIMAIAGAADWLHPPGVDLPDAAALARRSAMLPRPDSMPQPIYLRSADATPQPLAMN
                     EPLLRTAVAVDAAALALLHAECFDTAWSASEFASLMLMPGTEVLMAFDGPEPLAFLMT
                     RRAADEAEIVAIATRPGARRRGLARRLLDHQSTEFLAAGIATLFLEVASSNAAARGLY
                     ESLGFRPAGLRRNYYDRGGGLREDAVVMRKDLAPR"
     gene            complement(1352..1909)
                     /locus_tag="AC16A0_03630"
     CDS             complement(1352..1909)
                     /locus_tag="AC16A0_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3104451.1"
                     /translation="MFIQTEATPNPATLKFLPGKPVLPGGTRDYRTAEEAAESPLATR
                     LFSVNGVAGIFLGQDFITVTRGEAEWQHLKPAILGLIMDHYLSGAPVLLATSGGDSAS
                     EEFFDAADGEVVTTIKQLLDSRVRPAVAQDGGDITFHGFRDGIVYLNMKGSCAGCPSS
                     TATLKHGIENLLRHFVPEVAEVRAV"
     gene            2038..2385
                     /locus_tag="AC16A0_03635"
     CDS             2038..2385
                     /locus_tag="AC16A0_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3104452.1"
                     /translation="MQPDHLSLTLSALADPTRRAILARLAGGARSVTDLAEPFAMSLP
                     AVSRHLKVLERARLIERDRKAQWRPCRLAPEPLKDVSAWLDQYRRFWDESFDRLDTYL
                     TELQKGVPDEPAS"
     gene            2369..>2831
                     /locus_tag="AC16A0_03640"
     CDS             2369..>2831
                     /locus_tag="AC16A0_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3104453.1"
                     /translation="MNRQAKAQSAPADTLRITRVFAAPRALVYRMWTEKHLMDRWSCP
                     TGFTMPDSGADLRPGGTWHATMRTADGVNLKLQGTYREIVPDERLVFTHAWLGADGKP
                     GPETLVTVTFAEDGQNTRLTFEQGVFETVEACQSHTRGWGEFLERLDAYLTA"
BASE COUNT          517 a          920 c          944 g          450 t
ORIGIN      
        1 gattgctggc gatcagcacc ggcccttggc ggggcggctg accgatgaca cgcagcctga
       61 tgccgaggag gccgcagatc atacgatgat agtgcatggg aaaccggcgc gccatgccgg
      121 gccagaacca gacgaagagc tgttgcagcg gcatcagcgt cagcgtgacg gcggcgaaga
      181 cggtcagcag aacgaaggac cgcggcgtca tcgcggcgcc aggtccttgc gcatcaccac
      241 cgcatcctcg cgcaaaccgc cgccgcggtc atagtagttg cggcgcaggc ccgcaggcct
      301 gaagccgagg ctctcgtaga ggcctcgggc cgcggcattg gaggatgcca cttcaaggaa
      361 cagcgtggcg atgccagcgg caagaaactc cgttgactga tgatcgagca accgtcgcgc
      421 caggccgcgg cggcgggcgc cgggacgcgt tgcgatggcg acgatctctg cttcgtcggc
      481 cgccctgcgc gtcatcagga aagccagcgg ctccggtcca tcgaaagcca tgagcacctc
      541 cgtgcccggc atgagcatga ggctggcaaa ttcgctggca gaccaggcgg tgtcgaagca
      601 ttcggcatgg aggagggcca acgccgcagc atcgacggca actgccgtgc gcaagagcgg
      661 ttcattcatc gcaagcggct gcggtgtggc atcggccgaa cgaagataga tcggctgcgg
      721 catggagtcg ggccggggaa gcatcgcaga acgccgggcc aacgctgcgg cgtccggaag
      781 atcgactcca gggggatgaa gccagtccgc tgcaccggca attgccatga tccgctcggc
      841 cgcggtgccg agcaccacac ttcccggcgc caggcggcgt gcggcttccc cgagattcat
      901 cacctccggt ccgaagcggc gcgtgccatc cgcatcgaag ccagcggcat agacctcttc
      961 ctttcgcgca tcggcggcca caaggaccgg ccggcctggc ggcacattgg cggcaattgc
     1021 ggtcaggctg tcgaggccgg cgacggggat gcccagcgcc aggccaagcc cgcgcgccat
     1081 ggccaggccg atgcggacgc ccgtgaaagt tccaggcccc gctgtcaccg cgatacggct
     1141 gaggtcccgc ggatgaagct ccgcctcctg cagcacctcc gccaccatgg gcgccagcgc
     1201 ctctgcatga ccgcgctcca tcgccaccca gcggcgcgcg aggatgacgt cgcgatccgt
     1261 gtcgagaatg gcggccgagc aagccgccat ggacgtgtcg agggcgagga gcttcattgc
     1321 ggggccagtt aggccagccg cgggtcagcc gtcaaacggc gcggacttcc gccacttcgg
     1381 gcacgaaatg gcgcagcaga ttctcgatgc cgtgcttcag cgtggcggtc gagctggggc
     1441 agccggcgca ggagcccttc atgttgagat agacgatgcc atcacggaag ccatggaagg
     1501 ttatgtcgcc gccgtcctgc gccaccgccg ggcgcacccg gctgtcgagc agctgcttga
     1561 tggtcgtgac gacttcacca tcggccgcat cgaagaattc ttcagaagcg ctatcgccgc
     1621 cgctggtggc gaggaggacc ggcgcgccgg acaggtaatg atccatgatc agtccgagga
     1681 tggcaggctt caggtgctgc cattcggcct cgccgcgggt gacggtgatg aaatcctgcc
     1741 ccaggaagat gccagccacg ccatttaccg agaaaagccg tgtggcgagc ggtgactcgg
     1801 cagcttcctc ggccgtccgg tagtcgcgcg tgccgccggg aagaaccggc ttgccgggga
     1861 ggaacttcag cgtggcggga ttgggggtcg cttccgtctg gatgaacatg tccgattcct
     1921 cttgtcggac ttcatatagg tcagtttaga acggtttcaa agtgcggaga ttgcttgacc
     1981 gcagatcggc gatccgttat ttaacaattc agttaattaa ctgaatgttt aaattccatg
     2041 caacccgatc acctgagcct caccctgtcc gccttggccg atcccacccg gcgggcgatc
     2101 ctggcccggc tggccggcgg cgcacggagc gtgaccgatc tcgccgagcc ctttgccatg
     2161 agcctgcccg ccgtttcccg gcatctgaag gtgctggagc gcgcccggct gatcgaacgc
     2221 gaccgcaagg cgcagtggcg gccatgccgg ctggcgccgg aacctctcaa ggacgtttcc
     2281 gcctggctcg accagtatcg ccgcttctgg gatgagagct tcgaccgcct cgatacatat
     2341 ctgaccgaac tgcagaaggg agtgcccgat gaaccggcaa gctaaggcac agtccgcccc
     2401 ggccgatacg cttcgcatca cgcgagtctt cgccgcgcca cgggccctcg tctaccgcat
     2461 gtggaccgag aagcatctca tggaccgctg gagctgtccc accggcttca ccatgccgga
     2521 ctcgggcgcc gatctgcggc ccggcggaac gtggcacgcg acaatgcgga cggccgatgg
     2581 cgtcaatctg aagcttcagg gcacctatcg cgagatcgtg ccggacgaaa ggctggtctt
     2641 cacccatgcc tggctgggag cggatggcaa gcccggtccc gagacgctgg tcaccgtcac
     2701 cttcgccgag gacggccaga acacccgcct gaccttcgaa cagggtgtgt tcgagacggt
     2761 cgaggcctgc cagtcgcaca cccgcggctg gggcgagttc ctggagcggc tggacgccta
     2821 tctgacggcc g
//
LOCUS       DBNGVR010000169         2753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1241358, whole genome shotgun sequence.
ACCESSION   DBNGVR010000169 DBNGVR010000000
VERSION     DBNGVR010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1241358"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="AC16A0_03645"
     CDS             complement(<1..778)
                     /locus_tag="AC16A0_03645"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3104454.1"
                     /translation="MSSENSLVLTNIGLAFDHPEAVAWGPDGRVYAGGEAGQLYRFSL
                     DGKSCEEIARVPGGFLLGLAHDAAGNTYACDDRSACVHCIAPDGATSTYASGSDTQKM
                     RVPNYPVFDDHGNLYVSDSGTWGNRDGFIWKIAPGGRAEVWDRSCNGFTNGMCLSADG
                     KALYVAESSPPLVSRIEIGADGKAGRRTVVVELPRTVPDGLALDEAGNLYISLYNPNI
                     IYRLSPDGTLATLYDDWEQLMLIAPTNIAFGGPDMRTLIIA"
     gene            960..1736
                     /locus_tag="AC16A0_03650"
     CDS             960..1736
                     /locus_tag="AC16A0_03650"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104455.1"
                     /translation="MGLLDGKVCLVSGAGQGLGRAISREMAAEGAAVVLLERNGETVA
                     AVAQEIAKAGGTAHPYQLDVTDYDGYGKVVADVAMKLGRIDVLINNAAINPPTRTILN
                     DTLEDWRRTIAINLEAVYMGSKLVAPHMVARNDGRIIHVASIQGFASSGDCGSYNAAK
                     GGIIAYTKSMAVELGRYNILVNAVAPGFMVTPMSVVNGIDETTTPDFIDWYVTRRKIP
                     LGRSGLPEDVSGTVIFLASDYCRYMTGQLLVVDGGLMSTF"
     gene            1784..>2753
                     /locus_tag="AC16A0_03655"
     CDS             1784..>2753
                     /locus_tag="AC16A0_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104456.1"
                     /translation="MNITRKLNVSRRDIIRTSMMIGASSAFGSMLPRGAHAQEKTLDM
                     WWWGEQELPGLQKFVDGSIAGYKDATVKAMLQDTAVVISQFQTAAAAGNAPDIQYLWN
                     GIYHMESVWLGYLRGLNSLIKPDILKASNPTQLSHFGGDIYRVGWYPLPMFWCYNKDL
                     FEKAGLDPEAPPKTWDELLAACEKLKSAGIEPIGGGIQDGYWSEWYISHGLVQNLDTL
                     GEAVQLFIGDKDFREPKYHEHWVKLEELKNKGYLNKDMSSLELYPGIDLVVAGKVAIG
                     QTIGTRLPADSKATNGRIGYMTMPVYGKGKLAGTPVVDVQGLGISSN"
BASE COUNT          545 a          843 c          884 g          481 t
ORIGIN      
        1 tggcgatgat cagggtcctc atgtcaggcc cgccgaaggc gatgttcgtg ggggcgatca
       61 gcatcagctg ttcccagtca tcgtagagcg tggcgagcgt cccgtccggc gagagccggt
      121 agatgatatt gggattgtag aggctgatgt agagattgcc ggcttcgtcc agcgcaagcc
      181 cgtctggcac ggtgcgcgga agctcgacca cgaccgtccg ccggccggcc ttgccgtcgg
      241 cgccaatctc gatgcgcgac acgagcggcg ggctcgactc cgcgacatag agcgccttgc
      301 cgtctgccga gaggcacatg ccattggtga agccgttgca gctgcggtcc cacacctcgg
      361 ccctgccgcc gggcgcgatc ttccagatga agccgtcgcg gttgccccat gtgcccgagt
      421 cggagacgta gagattcccg tgatcgtcga agaccggata gttggggacc cgcatcttct
      481 gcgtgtccga tccgctggca taggtcgatg tggcaccgtc gggcgcgatg cagtgaacgc
      541 aggccgaccg gtcatcgcag gcataggtgt ttccggccgc atcatgcgcg agaccgagca
      601 ggaagccgcc ggggacgcgc gcgatctcct cgcagctctt gccgtcgagg ctgaagcgat
      661 agagctggcc cgcctcgccg cccgcataga cccgcccgtc tggtccccag gccacggctt
      721 ccggatgatc gaaggcaagg ccgatgttgg tgagtaccag gctgttttcc gatgacatga
      781 ggagggcctc gcgcggcagg ttgcgtcaag acaggagggg cgattgaacc ttgaccggtc
      841 tttcgtcaga actatagtgg cgtccagcaa tgcagatcaa tgtccaacat ttcggaactt
      901 gatcggagaa tggccggcag gctgccgcgg caggacctgc ttcgcacccg gggaacgtca
      961 tgggcttact tgacggcaag gtttgtcttg tgagtggcgc gggacagggc ctgggacgtg
     1021 cgatatcacg cgagatggcg gccgaaggtg cggccgtggt gttgctcgag cgcaatgggg
     1081 agaccgtcgc ggccgtggcg caggagattg caaaggccgg cgggaccgcg catccttacc
     1141 aactcgatgt gacggattac gacggatatg gcaaggttgt ggcggatgtg gccatgaagc
     1201 tcggccgcat cgacgtgctg atcaacaatg ccgcgatcaa tccgccgacc cgcacgatcc
     1261 tcaacgacac gctcgaggac tggcgccgca ccatcgcgat caacctcgaa gccgtctaca
     1321 tgggcagcaa gctggtggcg ccgcacatgg tcgcccgcaa tgacgggcgt atcatccacg
     1381 tcgcgtcgat ccagggcttt gcctccagcg gcgactgcgg gtcctacaat gccgcgaaag
     1441 gcggcatcat cgcctacacg aagtcgatgg ccgtggagct tggccgctac aacattcttg
     1501 tcaatgccgt ggcgcctggc ttcatggtga cgccgatgtc ggtggtcaat ggcatcgacg
     1561 agacgacgac ccccgacttc atcgactggt acgtcacccg ccggaagatt ccgctcggac
     1621 gcagcgggct tcctgaagat gtctccggca ccgtgatctt tctggcgtcg gactactgcc
     1681 gctacatgac gggccagctt ctggtcgtcg atggcggcct gatgagtaca ttctgaaatc
     1741 gcaccacaag aacaatcgag gaaacaggga aggcaaattg aaaatgaaca tcactcgcaa
     1801 actcaacgtc tcgcggcgcg acatcatccg cacctccatg atgatcggcg ccagctcggc
     1861 cttcggcagc atgctgcccc gcggcgccca tgcccaggag aagaccctgg acatgtggtg
     1921 gtggggcgaa caggaattgc ccgggctgca gaagttcgtc gacggctcga tcgccggtta
     1981 caaggacgcg accgtcaagg ccatgttgca ggacacggcc gtggtcatct cgcagttcca
     2041 gaccgccgcg gcagccggca acgcgcccga catccagtac ctctggaatg gcatctatca
     2101 catggagagc gtctggctcg gctacctgcg cggcctcaac agcctgatca agccggatat
     2161 cctgaaggcc tccaatccga cgcagctcag ccatttcggc ggagacatct atcgcgtcgg
     2221 ctggtacccg ctgccgatgt tctggtgcta caacaaggac ctcttcgaga aggccgggct
     2281 cgaccccgaa gcgccgccga agacctggga cgagctgctt gcggcctgcg agaagctcaa
     2341 gagcgccggc atcgagccga tcggcggcgg catccaggat ggctactgga gcgaatggta
     2401 catcagccat ggcctggtgc agaacctcga cacgctgggc gaggccgtcc agctcttcat
     2461 tggcgacaag gacttccgcg agccgaaata ccacgagcac tgggtcaagc tcgaggagct
     2521 gaagaacaag ggatatctca acaaggacat gtcctcgctc gaactctatc ccggcatcga
     2581 tctcgtggtc gctggcaagg tggcgatcgg ccagaccatc ggcacaaggc ttcccgccga
     2641 cagcaaggcc accaatggcc gcatcggcta tatgaccatg ccggtatacg gcaagggtaa
     2701 gctggccggc acgccggtcg tcgacgtaca ggggcttggc atatcgagca acg
//
LOCUS       DBNGVR010000170         2486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_651820, whole genome shotgun sequence.
ACCESSION   DBNGVR010000170 DBNGVR010000000
VERSION     DBNGVR010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_651820"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16A0_03660"
     CDS             <1..399
                     /locus_tag="AC16A0_03660"
                     /inference="COORDINATES: protein motif:HMM:NF020253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR3104457.1"
                     /translation="GTTTPIDVGLDGLVSKKKADFIGKRSLEQAYLKGPNRKQLVGLL
                     TEDPNEVLPDGAYAVKAVKDKPPMEMIGQVTSTYMSPSLGRSIAMALIENGRARMGET
                     ISFPLEGNKVMRARIVDPVFYDKEGSKLNV"
     gene            392..994
                     /locus_tag="AC16A0_03665"
     CDS             392..994
                     /locus_tag="AC16A0_03665"
                     /EC_number="1.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF016180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:1901053 - obsolete sarcosine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma"
                     /protein_id="HIR3104458.1"
                     /translation="MSELFYESPLAGAAAAEGLSVSLREIPDRGMIDLRGLTSDRKFM
                     AAAKEALSIDLPRQPRSSVAWGDIKLLWLSIDQWLILCPRGKVTDVIARLRDALGNIH
                     SLVVDVSDMRAVIRMEGEGCREIIMKGTSLDLLSAEYAPGLVRRMRFAEIAALLNVVE
                     DDVFDIYVFRSYAHYAWDFLRAAAREPARVKLFGKQVAPA"
     gene            1060..2373
                     /locus_tag="AC16A0_03670"
     CDS             1060..2373
                     /locus_tag="AC16A0_03670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR3104459.1"
                     /translation="MTAAAVLAPRPALAQSPPQVLFSNVRVFDGISPGLSAPRHVMVR
                     GNLISELSETPLSAGPGTTVIDGGGRTLMPGLIDAHVHMAMSTLPIALMMTADPNYST
                     LRTGRAAEDMLLRGFTAVRDLGGPTFGLKRAIDEGFVAGPRIWPCGATISQTSGHGDF
                     RLVSDLPRSDAAPLHATERYGYAAIADGVPEVLRRVREQLMQGASQIKIMAGGGISSN
                     FDPLDVTQYTEDEMRAAVAAAADWGTYVTVHAYTPRAVQAAIRAGVRCIDHGQLLDEE
                     TVRIMADKGLWWSLQPFIDDGDSRFPEGSPNRVKQLEMYNGTDTAYALAKKHGIKVAW
                     GTDILFDQMAAARQGQHITRMTRWYDAPDILRMVTAGNAELLQLSGNRSPYPGRLGVV
                     QEGALADLLLVNGDPLANLALLENTQASLAVIMKDGKIYKNALGG"
BASE COUNT          436 a          838 c          789 g          423 t
ORIGIN      
        1 ggcaccacca cgcccatcga cgtcgggctc gacggtctgg tgtcgaagaa gaaggccgat
       61 ttcatcggca agcgctcgct ggaacaggcc tatctcaagg gcccgaaccg caagcaactg
      121 gtgggcctgc tcacggaaga cccgaacgag gtccttcccg acggcgccta tgcggtgaag
      181 gcggtgaagg acaagccgcc aatggaaatg atcggccagg tcacatcgac ctacatgtcg
      241 ccgtcgcttg gccgctcgat tgccatggcg ctgatcgaga acggccgggc gcgcatgggc
      301 gagaccatct ccttcccgct ggaaggcaac aaggtcatgc gcgccaggat cgtcgatccg
      361 gtcttctacg acaaggaagg atccaagctg aatgtctgag ctcttctacg aatccccgct
      421 tgcgggcgcg gctgcggccg aaggcctctc ggtcagcctg cgcgagatac ccgaccgcgg
      481 catgatcgac ctgcgcggcc ttacgtccga ccgcaagttc atggctgccg caaaggaggc
      541 gctcagcatc gatcttccca ggcagccgcg cagctccgtc gcctggggcg acatcaagct
      601 gctctggctg tcgatcgacc agtggctgat cctctgcccg cgcggcaagg tcacggatgt
      661 catcgccagg ctgcgcgatg cgctgggcaa catccattcg ctcgtggtcg acgtgtccga
      721 catgcgcgcg gtgatccgca tggaaggcga aggctgccgc gagatcatca tgaagggcac
      781 ttcgctggac ctcctgtccg ccgagtatgc gcccggcctg gtgcgccgga tgcgcttcgc
      841 cgagatcgcc gcgctgctca acgtggtgga ggacgatgtc ttcgacatct acgtcttccg
      901 gtcctacgcg cattatgcct gggacttcct gcgcgctgca gcacgcgagc ccgcccgggt
      961 gaagctcttc ggaaagcagg ttgcgccagc ctgacggcct gaccatactc ccgtttcgag
     1021 gaggaacgga tcatagtcac gcgtcgtcac ctgcttggca tgacggccgc cgccgtcctg
     1081 gcgccgcggc cggcacttgc gcaatccccg ccgcaggtcc ttttcagcaa tgtccgcgtg
     1141 ttcgatggca tctcgccagg gctctcggcg ccgcggcatg tgatggtccg cggcaatctg
     1201 atatcggaac tctccgagac accactgtca gccggccccg gcaccacggt catcgacggc
     1261 gggggccgca cgctgatgcc gggcctcatc gatgcccatg tccacatggc catgtccacc
     1321 ctgcccatcg cgctgatgat gacggccgat ccgaactaca gcacgctgcg caccgggcgg
     1381 gccgccgaag acatgctgct gcgcggcttc accgcggtgc gcgatctcgg cggacccacc
     1441 ttcgggctga aacgcgccat tgacgagggc ttcgtcgccg ggccgcgaat atggccctgc
     1501 ggcgccacca tctcgcagac ctccgggcac ggcgacttcc ggcttgtgtc cgatcttccg
     1561 cgctccgatg cggcgcccct gcacgccacc gaacgctatg gttacgcagc gattgccgat
     1621 ggcgtgccgg aggtgctccg ccgggtgcgc gaacagctga tgcagggcgc aagccagatc
     1681 aagatcatgg ccggcggcgg aatctcgtcc aacttcgacc cgctcgacgt cacccagtac
     1741 accgaggatg aaatgcgcgc ggcggtggcc gcggcggcgg actggggcac ctatgtcacg
     1801 gtccatgcct atacgccacg agccgttcag gcggccatcc gtgcgggcgt ccgctgcatc
     1861 gaccacggcc agctgctcga cgaggagacc gtgcgcatca tggccgacaa gggcctgtgg
     1921 tggagcctgc agcccttcat tgacgacggc gactcgcgtt ttcccgaggg atctcccaat
     1981 cgagtgaagc agctggagat gtacaacggg acggacacgg cctatgcgct ggcgaagaaa
     2041 cacgggatca aggtggcctg gggcaccgac atcctcttcg accagatggc cgcggcccgg
     2101 cagggtcaac acatcacccg catgacccgc tggtacgacg cgcccgacat cctgcgcatg
     2161 gtcacggccg gcaatgccga actgcttcag ctctccggca atcgcagtcc ctatcccggc
     2221 agactgggcg tggtgcagga gggagcgctc gccgatctgc tcctcgtcaa cggggacccc
     2281 cttgcgaatc tcgccttgct cgagaacacg caagcgtcgc ttgccgtgat catgaaggac
     2341 gggaagatct acaagaacgc gcttggcggc tgaggtctct cccaccccct gagaaggagg
     2401 gagagtctga ccgggaccgc cggcccgatc aggagtcttc cttcaggttg cccggctgct
     2461 tgatgctgca gcttgccttc agcggt
//
LOCUS       DBNGVR010000171         2408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4384742, whole genome shotgun sequence.
ACCESSION   DBNGVR010000171 DBNGVR010000000
VERSION     DBNGVR010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4384742"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1037)
                     /locus_tag="AC16A0_03675"
     CDS             complement(60..1037)
                     /locus_tag="AC16A0_03675"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIR3104460.1"
                     /translation="MTRICIFGAGAIGGYVGARLAMAGEADVSLVARGPHLAAMQQHG
                     LILKQDGETRVLHPEVTDDPRQLGPQDFIILTLKAHAITGVIEALQPLVGPHTAILFA
                     QNGIPWWYFYKSGGPFDGHRLQSVDPGGAIWDRIGPERALGCVVWQAAEIEAPGVISH
                     HYGDRMPIGEPSGEKTARVQTLSRLLTAAGIKSQIKPNLRNEIWLKLWGNLSFNPVSV
                     LTQGTLVELASHAGTRRVIRQMMQEAGDVAQMLDVTFPVGVDERIGMAAKVGAHRTSL
                     LQDVEAGRPTELDALLGVVIELAALVGRPVPALQMVYDLAKFRCSPPDR"
     gene            1243..>2408
                     /locus_tag="AC16A0_03680"
     CDS             1243..>2408
                     /locus_tag="AC16A0_03680"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3104461.1"
                     /translation="MTDRLTIALAQLNPTVGAINANLAKARDALAKSAGADLILFPEL
                     FICGYPPEDLVLRPSFVENCRIAVEDLARDTASGPAILMGLPWRDGSRLYNAVALLAN
                     GRIETLRYKHDLPNYGVFDEKRVFDAGPAPGPISFKGVRLGVPICEDIWTEESCETLA
                     ETGAEILLVPNGSPFEGNKDDVRLNLVVARVTETGLPLVYLNQVGGQDELVFDGASFV
                     LNADRSLALQMPMFEEGLALTEWRRDSGGWRMQPGDIARLPEAEEETWKACVLGLRDY
                     VLKNRFPGVVLGLSGGIDSAVVAAMAVDALGPERVHCVMMPYAYTSRDSLVDAEDCAR
                     RLGVRYDIVPIKEPVEGFLSALKPLFEGTPSGITEENLQSRARGVILMAISNKFG"
BASE COUNT          422 a          776 c          775 g          435 t
ORIGIN      
        1 ctcgatgttg ctgctgggct tctcattgtc gagacgcatg ggacgttctc ctggataact
       61 taccggtcag gcgggctgca tctgaatttg gcaagatcat agaccatttg caaggcgggt
      121 acagggcgcc cgaccagcgc ggccaactcg atcacgacgc caagcaatgc atccagttcc
      181 gtgggccttc ccgcctccac atcctgtagc agagaggtgc ggtgggcgcc caccttcgcc
      241 gccataccga tgcgctcgtc gacaccgacc gggaatgtga cgtccagcat ctgcgcgacg
      301 tctccggctt cctgcatcat ctgccttatc acccggcgag ttcccgcatg acttgcgagt
      361 tccaccagcg tgccctgcgt cagcacgctg accgggttga agctgagatt gccccagagc
      421 ttgagccaga tctcattgcg caggttcggc ttgatctgac ttttgatgcc ggccgccgtc
      481 aggaggcggc tcagcgtctg aacgcgtgcg gtcttttcgc cggacggttc gccgatcggc
      541 atgcggtcgc cataatggtg ggagatgacg cccggcgcct cgatctcggc tgcctgccag
      601 acgacgcagc caagcgcgcg ttccggcccg atgcggtccc agatcgcgcc gccgggatcg
      661 acgctctgca gccgatggcc gtcgaacggt ccgcccgact tgtagaagta ccaccagggg
      721 atgccgttct gtgcgaagag aatggcggtg tgcggaccca ccagaggctg cagcgcctcg
      781 atgacacccg tgatcgcatg agccttgagg gtcagaatga tgaaatcctg cgggcccagc
      841 tggcgcgggt catcggtgac ctcagggtgc aggacgcgcg tctcgccatc ctgcttcagg
      901 atgaggccat gctgctgcat cgcggcaaga tgaggcccgc gggcaaccag cgacacgtcg
      961 gcttcgccgg ccatcgccag cctggcgccg acatagccgc cgatcgcacc ggcgccgaag
     1021 atgcagatgc gcgtcatgcc agcccccgcc cccgcgcttg tctgggaatc gcgcaacggc
     1081 catatgcacg atgacggcgc ctggggacca tgcaggccgc tgcagcaacc gggccagacc
     1141 ttgagaggaa ggcggggatc atcggcctct catgcgcaat tgtgttgccg ccgtcaacgg
     1201 cgcgcaattg aaggcgcccg gccaatccgc taagaccgcg ccatgaccga caggctcacc
     1261 atcgcactgg cgcagctcaa cccgaccgtt ggcgccatca acgccaatct ggcaaaggcg
     1321 cgcgatgcgc tggcaaagag cgccggagcc gatctgatat tgtttccgga gctcttcatc
     1381 tgcggctatc cgccggagga tctggtgctg cgcccgtcct tcgtggagaa ctgcaggatc
     1441 gccgtggaag acctcgcgcg cgacacggcg tccggtccag ccatcctcat gggactgccc
     1501 tggcgagacg gaagcaggct ctacaatgct gttgcgctgc tcgccaatgg ccggatcgag
     1561 acgctgcgct acaagcatga cctgcccaac tacggcgtct tcgacgaaaa gcgggtcttc
     1621 gatgccggcc ccgctcccgg acccatctcc ttcaagggcg tgcggcttgg cgtgcccatc
     1681 tgcgaggata tctggaccga ggagtcctgc gagacgctgg ccgaaaccgg tgccgagatc
     1741 ctgctggtgc cgaacggctc gcccttcgag ggaaacaagg acgatgtacg cctcaatctg
     1801 gtggtggctc gggtgaccga gaccggtctg ccgctcgtct acctcaacca ggtgggcgga
     1861 caggacgagc tcgtcttcga cggcgcttcc ttcgtgctca atgccgaccg gtcgctcgcc
     1921 ctgcagatgc cgatgttcga ggagggcctc gccctcaccg aatggcggcg cgattccggt
     1981 ggctggcgca tgcagccggg tgacattgca aggctccccg aagcggagga ggagacctgg
     2041 aaggcctgcg tgctgggcct gcgcgactat gtgctcaaga accgctttcc tggcgtggtg
     2101 ctgggactct cgggtggaat cgactcggcc gtggttgccg cgatggcggt ggatgcactg
     2161 gggccggaac gcgtgcactg cgtgatgatg ccttacgcct ataccagccg cgacagtctc
     2221 gtggatgccg aggactgcgc caggcgactc ggtgttcgtt atgacatcgt gccgatcaag
     2281 gaacctgtcg aaggctttct ctcggccttg aagccgctgt tcgaaggaac gcccagcggc
     2341 atcaccgagg agaacctgca gtcacgcgcg cgcggcgtga tcctgatggc gatttccaac
     2401 aagttcgg
//
LOCUS       DBNGVR010000172         2635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3796528, whole genome shotgun sequence.
ACCESSION   DBNGVR010000172 DBNGVR010000000
VERSION     DBNGVR010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3796528"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="AC16A0_03685"
     CDS             complement(<1..500)
                     /locus_tag="AC16A0_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3104462.1"
                     /translation="MQDILEKLERKRAEARAGGGERRVAAQHAKGKLTARERIELLLD
                     EGSFEEFDMFVEHRCTEFGMEGTKFAGDGVVTGWGTVNGRVVYVFAKDFTVFGGSLSE
                     SHARKMIKIQDMALQNRAPIIGLFDAGGARIQEGVDALGGYGEVFARNVNSSGVIPQI
                     SFIMGP"
     gene            complement(574..1269)
                     /locus_tag="AC16A0_03690"
     CDS             complement(574..1269)
                     /locus_tag="AC16A0_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family chaperone protein"
                     /protein_id="HIR3104463.1"
                     /translation="MKSRRFYKTVSVTGDLGIALDGRLVKTPGKVPLLLPLPALAQAV
                     AAEWEAQGETIDPASMVMTRLANTAIDRVPAHRPAILAEMRDFAGSDLVCYRAAEPDD
                     LVARQSAAWDPVIDGAVAELGQPIAIWSGVMHRPQPAATLEAVSRWCAKLSDFEIAAF
                     HSLMTLTGSALIPMMLTKRAIAPDQAWAAAHVDEDFQIEAWGQDDEAAARRRRRHAEF
                     DACCRFLLLAASN"
     gene            complement(1266..1937)
                     /locus_tag="AC16A0_03695"
     CDS             complement(1266..1937)
                     /locus_tag="AC16A0_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3104464.1"
                     /translation="MRLAIFDVDGTLVDSRSMITAAMTRAFADVGIAAPDQDRMLSIV
                     GLSLVDAMAALTTDESTATHRRLGEAYKQAFWDLRLSGAHPEALFNGARDLLEDLRGR
                     GVVLGIATGKSRRGVDHLIAAHGFANWFATVQTSDTHPSKPHPSMIVTALAETGCDPA
                     ATIMIGDTSFDMSMARQAGVAAAGVAWGNHPATELVAAGAHRVSKNFKQLALDLEALW
                     EERGR"
     gene            complement(1934..>2635)
                     /locus_tag="AC16A0_03700"
     CDS             complement(1934..>2635)
                     /locus_tag="AC16A0_03700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3104465.1"
                     /translation="VIHEDGDLFVLDKPSGFAVQGGTKTHHHLDGLLMGLGVEVGERP
                     LLVHRLDRDTSGVIVVAKRRAVASALGKLFATRAVKKTYWAVTRGVPKPAQGRIEVAL
                     IKAKGLDGDRMRASKEGEEDDEQRAVTHYAVIDKAPPVAAWVSLKPVTGRQHQLRAHM
                     AHIGTPILGDNKYGGDLELPEGVADRLHLHARRIIFPHPRGGTVDVSAPLPTHMRDTF
                     ALFGFDADRFEEETP"
BASE COUNT          447 a          881 c          860 g          447 t
ORIGIN      
        1 cacgggccca tgataaatga aatctgcggg atcacgcccg aggaattgac gttgcgcgcg
       61 aacacttcgc catatccgcc aagcgcatcg acgccttcct gaatgcgcgc accgccggca
      121 tcgaacagac cgatgatcgg cgcgcggttc tgcagcgcca tgtcctggat cttgatcatc
      181 ttgcgggcgt gggattccga cagcgagccg ccgaagacgg tgaagtcctt ggcgaagaca
      241 tagacgaccc ggccattcac ggtgccccag cccgttacca cgccatcgcc ggcgaacttc
      301 gtgccttcca tgccgaactc ggtgcagcgg tgttcgacga acatgtcgaa ctcctcgaag
      361 ctgccctcgt cgagcagaag ctcgatacgc tcgcgcgccg tgagcttgcc cttggcatgc
      421 tgggcggcca cgcggcgttc gccaccgccg gcgcgtgcct cggcacgctt gcgctcgagc
      481 ttttccagaa tgtcctgcat gatgccctcg ttcctgctgt tgccaacttg ttaggcggca
      541 gcggagacgg cgtcaaatcg gccgaaaggc caatcagttg ctggcggcca gcaggaggaa
      601 gcggcagcag gcatcgaact cggcatgacg gcgcctgcgg cgcgcggctg cctcatcatc
      661 ctggccccag gcctcgatct ggaagtcctc gtcgacatgg gcggcggccc aggcctggtc
      721 gggcgcgatc gcccgcttgg tcagcatcat gggaatgagg gccgagcccg tcagcgtcat
      781 gagggagtga aacgcggcga tctcgaaatc agacagtttc gcgcaccagc ggctgaccgc
      841 ctccagcgtc gccgccggct gcgggcgatg catgacaccg gaccagatcg cgatcggctg
      901 gccgagttcc gcaaccgccc cgtcaatgac cggatcccat gcggccgact ggcgggccac
      961 cagatcgtcg ggctcggcgg cgcgatagca gacgagatcg ctgcccgcga aatcgcgcat
     1021 ttcggcgaga atggcaggcc ggtgggccgg cacgcggtca atcgcagtat tggccaggcg
     1081 ggtcatcacc atggaggccg gatcgatggt ctcgccctga gcctcccact cggccgcgac
     1141 ggcctgcgcg agggccggaa gaggcaggag gagcggcacc ttgccgggag tcttcaccag
     1201 ccggccatcg agcgcgatgc caaggtcccc ggtgacggat accgtcttgt agaagcgccg
     1261 gctcttcatc gtccccgctc ttcccacaaa gcctcaagat caagcgccaa ttgcttgaaa
     1321 ttcttggaaa ccctgtgggc acctgcggcc accagctcgg ttgcgggatg gttgccccag
     1381 gccacgcctg ccgccgccac cccggcctgc cgggccatgg acatgtcgaa actggtgtcg
     1441 ccgatcatga tggtggctgc cggatcgcag cccgtctcgg ccagagcggt cacgatcatc
     1501 gaggggtgtg gctttgacgg atgcgtgtcc gaggtctgta ccgttgcgaa ccagttcgcg
     1561 aagccatggg cggcaatgag gtggtccacg ccgcggcgcg acttgccggt ggcgatgccg
     1621 aggaccacgc cccggccccg caggtcttcc aaaaggtccc gcgcaccatt gaacagggcc
     1681 tccggatggg cgccggacag acggagatcc cagaaagcct gcttgtaggc ctcccccagg
     1741 cgccgatggg tcgctgtaga ctcatccgtc gtcaaggctg ccatcgcgtc caccagcgag
     1801 agcccgacga ttgacagcat ccggtcctgg tccggcgcgg cgatgccgac atcggcaaag
     1861 gcgcgcgtca ttgcggcggt gatcatgctg cggctgtcca ccagcgtgcc atccacgtcg
     1921 aagatggcga gcctcacggt gtctcctcct cgaacctgtc ggcgtcgaag ccgaagagcg
     1981 cgaaggtgtc gcgcatgtgg gtcggcagtg gcgccgagac atcgacggtt ccgccgcgcg
     2041 gatgcgggaa gatgattcgc cgcgcatgca ggtgcaggcg gtcggcgacg ccctcgggaa
     2101 gctcgaggtc gccaccatac ttgttgtcgc ctaggatcgg ggtgccgata tgcgccatgt
     2161 gggcgcgcag ctggtgttgg cgtccagtca ccggcttcag cgacacccag gctgccactg
     2221 gcggcgcctt gtcgatcacg gcataatggg tcaccgcgcg ctgctcgtcg tcctcttcgc
     2281 cctctttgct cgcgcgcatg cggtcgccat ccagaccctt ggccttgatc agcgccactt
     2341 cgatccgccc ctgtgcgggc ttgggcacac ccctggtcac ggcccagtag gtcttcttca
     2401 cggcgcgcgt tgcaaacagc ttgcccagcg ccgaagccac cgcccggcgc ttggccacca
     2461 cgatgacgcc cgaggtgtcg cggtcgaggc gatgcaccag cagcggacgt tcgccaactt
     2521 cgacgcccag ccccatcaga aggccgtcga gatggtgatg ggtcttggtg ccgccttgaa
     2581 cggcaaagcc gcttggcttg tcgaggacga agaggtcgcc gtcctcgtgg atcac
//
LOCUS       DBNGVR010000173         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2421408, whole genome shotgun sequence.
ACCESSION   DBNGVR010000173 DBNGVR010000000
VERSION     DBNGVR010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2421408"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="AC16A0_03705"
     CDS             complement(<1..1650)
                     /locus_tag="AC16A0_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3104466.1"
                     /translation="MTDHRPDRSLVKYPESGARIALRVSLKLALLAVFVVITNVGFGQ
                     RLSMLYETGRWETLIPYLIIWATALVAVAVAAFHPKTWVRLVWAAVMTLSSAVAWGYH
                     NASQSEFTVFDIVSLWNARHEAGRAADFYGEHIMLASVLLVCGILIFALPVGTRGPLR
                     PWIKRLGWLPVLPIAVIGAIVVMKGGGGSQAMPSQFTPVALTSLAGAKIALQGAPVRR
                     QVTWQPQPGPRRNIVMMIDESIRHDYLDFTAGNPHTPRLAALADRFVNFGPAVSGGNC
                     SNYSNAILRFAASRKDVTGSINTSPTLWSYAKKAGYRTVFIDAQAGGITNPGLMQNYM
                     TMTERADIDAFHALRDVTSAEADFRLAEIVAAELAKPGPVFIYANKNGAHFPYDAAYP
                     ESEAHYRPTMTEAGVDDPVARVASYRNAVAWSVDRFMADLFAKADFSDTTMVYTSDHG
                     QALDPGKLSHCVVDNPDARTALVPLLVATDDAVLQAELAAAARQRHGKASHFQIAPSV
                     LSWMGYGDSDIATAYDESLVSGNREPVFTAGDVFGLFSSAVH"
     gene            1762..>1975
                     /locus_tag="AC16A0_03710"
     CDS             1762..>1975
                     /locus_tag="AC16A0_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104467.1"
                     /translation="MTEVQNAVTAALVREQLADAAEIGWACSWLEACGYPGLKLLDEA
                     LQDAQRDLPLVRDALGLDLQNVSCAFL"
BASE COUNT          367 a          589 c          695 g          324 t
ORIGIN      
        1 gtgtaccgcg ctggaaaaga gcccgaagac atcgcccgcg gtgaaaaccg gttcgcgatt
       61 gccgctgacc agggattcgt cataggccgt ggcgatgtcg ctgtcgccat agcccatcca
      121 ggacagcacg ctgggcgcga tctggaagtg cgaggccttg ccgtggcgct gccgcgccgc
      181 agccgcgagt tcggcctgca ggacggcatc gtcggttgcc accagcaacg gcacaagggc
      241 cgtgcgcgca tcgggattgt cgaccacgca atggctgagc tttccgggat cgagcgcctg
      301 gccatgatcc gaggtgtaga ccatcgtggt gtcggagaaa tcggctttgg cgaatagatc
      361 cgccatgaac ctgtcgaccg accaggccac ggcattgcgg taggaggcga cacgtgccac
      421 cggatcgtcg acgccggctt ccgtcatggt gggccgataa tgtgcctcgc tttcgggata
      481 ggcggcgtca taggggaaat gcgcgccatt cttgttggcg tagatgaaga cggggccggg
      541 cttggccagt tccgcggcga cgatctcggc caggcggaaa tcggcctcgg cgctggtcac
      601 gtcgcgcagg gcatgaaagg cgtcgatgtc ggcgcgctcg gtcatggtca tgtagttctg
      661 catcaggcca ggattggtga tgccgcccgc ctgggcatca atgaagacgg tgcggtagcc
      721 ggccttcttc gcataggacc acagggtggg gctggtgttg atcgagccgg tcacatcctt
      781 gcgggatgcg gcaaagcgca ggatggcatt ggagtagttg ctgcagttgc cgcccgagac
      841 ggcgggaccg aagttgacga agcggtccgc cagcgcagca aggcgcgggg tgtggggatt
      901 gccggccgtg aagtccaggt agtcgtggcg gatgctctcg tcgatcatca tcacgatgtt
      961 gcgccggggg ccaggctgcg gttgccaggt gacctgacgg cggacgggcg cgccctgcag
     1021 cgcgatcttg gcgcccgcaa gcgatgtgag cgccacgggc gtgaactgcg agggcatggc
     1081 ctgggagccg ccgccgccct tcatcaccac gatggcgccg atgaccgcga tgggcagcac
     1141 gggcagccag ccgagtcgct tgatccaggg ccgcagtggg ccgcgggttc cgaccggcag
     1201 cgcgaaaatg aggataccgc agaccagcag cacggaagca agcatgatgt gctcgccata
     1261 gaagtcggcc gcgcgtcccg cttcgtggcg ggcgttccac aatgagacaa tatcgaagac
     1321 ggtgaactcc gactggctgg cattgtgata gccccaggcc accgcgctgg agagcgtcat
     1381 gacggcagcc cagacgaggc ggacccaggt cttcggatgg aaagccgcca cggccacggc
     1441 cacgagcgcc gtggcccaga tgatcagata gggaatgagc gtctcccagc ggcccgtctc
     1501 gtagagcatg gacaggcgct ggccgaagcc gacattggtg atgacgacga aaaccgccag
     1561 aagcgccagc ttcaggctga cgcgcagggc gatgcgcgcg ccactttcag ggtacttcac
     1621 cagggaacgg tcgggccggt ggtcagtcat cggcaggtca tcctcgtcaa agacagtttg
     1681 gtggaccatt cggtgcaaca aacccgccaa catggacggc acggcgaaac ttggttaatc
     1741 actggtgtca tgcacaggac gatgacagag gttcaaaacg cggtgaccgc cgccctggtc
     1801 cgggagcagc ttgccgacgc cgccgaaatt ggctgggcct gcagctggct cgaggcctgc
     1861 ggctatccgg gcctcaagct cctggacgag gccttgcagg atgcacagcg cgacctgccg
     1921 ctcgtccgcg atgcgctggg gctggacctg cagaacgtct cctgcgcctt cctgg
//
LOCUS       DBNGVR010000174         2492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3993564, whole genome shotgun sequence.
ACCESSION   DBNGVR010000174 DBNGVR010000000
VERSION     DBNGVR010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3993564"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="AC16A0_03715"
     CDS             complement(<1..939)
                     /locus_tag="AC16A0_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3104468.1"
                     /translation="MKKRLFKAPSGQSVSFTELGFGAAPLGNLYRAVSDKDAQDTLTA
                     AWKAGIRYFDTAPLYGLGLSEIRLNQFLRGKARKDYVISTKVGRLLEVCPPAERTGIG
                     KFFDTPSRKERFDYSYDGVMRSLEFSLERLGIDSIDVVFAHDVDVFTHKSEAARDKHV
                     ASLMKGGYKALVKLRDERVIKAIGAGINEWQVAETLARSGDFDVFLLAGRYTLLEQEA
                     LRSFLPLCAERGIGIVLGGPYNSGILATGPKPGAYYNYDPAPKHILDRVRRIEAVCKA
                     HKVKLPEAALRFPLMHPCVVSVIPGAVTPKEVALNVK"
     gene            complement(936..1670)
                     /locus_tag="AC16A0_03720"
     CDS             complement(936..1670)
                     /locus_tag="AC16A0_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3104469.1"
                     /translation="MSAVAVTYDFLSPDRPTGKTGATARAHALLREAIVSLELKPGDP
                     LDKQAIAARLGVSRFPVAEAMGRLAAEGLVEVIPQSGSRVALIRIADARENMFLRRAL
                     EVETARRVARAPSPELAEALRQNLRYQKAAIDSGDPKGFHHFDLAFHAMLQDQLGFSR
                     VRNATETARLGLERVRRMLNTRRRLDVTLAEHEAIARAISAGDGQAAASAMNDHLESV
                     LAELEDFARERPELFADLTFRGGPSQ"
     gene            1730..>2492
                     /locus_tag="AC16A0_03725"
     CDS             1730..>2492
                     /locus_tag="AC16A0_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR3104470.1"
                     /translation="MIATVPKTPRMLRLTPEDNVLVAIDVVEKGATAPEGIVALERIM
                     KGHKMAATEIRVGQPVRKFGQIIGFAKQDIPKGAWIHEHNTGMQDFDRDYAFAEEAKP
                     EDILPLGQQATFQGYRRANGKVGTRNYIGIMTSVNCSATVAGFIAEEVKRSGMLKDYP
                     NIDGIVALKMDNGCVVDFRGAIFDILKRTAWGYATNPNMGGVLMVGLGCEGFQIPRFK
                     EAYNVTESETFRTMTIQETGGTRKTVAAGVAAVREM"
BASE COUNT          455 a          798 c          828 g          411 t
ORIGIN      
        1 cttcacgttg agggccacct ccttgggggt caccgcgccc ggaatgacgc tgaccacaca
       61 agggtgcatg agcggaaagc gcagcgccgc ctcgggcagt ttcaccttgt gggccttgca
      121 gacggcctcg atacggcgga cgcggtcgag gatgtgcttg ggcgccgggt cgtagttgta
      181 gtaggcgccg ggtttgggtc cggtcgccag aatgcccgag ttgtagggtc cgcccagcac
      241 gatgccgatg ccacgctccg cgcagagcgg caggaaggac ctgagcgcct cctgctccag
      301 cagcgtgtag cggcccgcca gaaggaagac gtcgaagtcg ccgctgcgcg ccagcgtttc
      361 cgccacctgc cactcgttga tgccggcgcc gatcgccttg atcaccctct cgtcgcgcag
      421 cttgaccagc gccttgtaac cgcccttcat caggctggcg acatgcttgt cgcgggcggc
      481 ctccgacttg tgggtgaaga catcgacgtc atgggcgaag accacatcga tcgagtcgat
      541 gccgagccgc tccagcgaga attcgagcga gcgcatgacc ccgtcgtagc tgtagtcgaa
      601 acgttccttg cgcgagggcg tgtcgaagaa cttgccgatg ccggtgcgtt cggcgggcgg
      661 gcagacctcg agcagacgcc cgaccttggt ggagatcacg tagtccttgc gcgccttgcc
      721 gcgcaggaac tggttgagcc ggatctccga caggccgagg ccatagagcg gcgccgtatc
      781 gaaatagcgg atgcccgcct tccaggcggc agtgagcgtg tcctgcgcat ccttgtcgct
      841 gactgcacga tagagattgc caagcggtgc tgcaccgaac cccagttcgg tgaagctgac
      901 gctctggccc gagggcgcct tgaacaggcg tttcttcatt gtgatggtcc tcccctgaaa
      961 gtcagatctg cgaagagctc gggccgctcc cgggcaaagt cttcgagttc ggccagcacg
     1021 gactccagat gatcgttcat ggccgacgcc gcagcctggc cgtcgcccgc cgagatagca
     1081 cgggcgatgg cttcgtgctc ggccaacgtc acgtccagtc ggcggcgcgt gttgagcatg
     1141 cggcgcacgc gttcgaggcc aagccgtgcc gtctcggtgg cgttgcgcac ccggctgaag
     1201 ccgagctggt cctgcagcat ggcatggaag gcgagatcga agtggtggaa gcccttggga
     1261 tctccgctgt cgatggcggc cttctggtag cgcaggttct ggcgcaatgc ttcggcgagt
     1321 tcgggcgagg gcgcccgggc gacccggcgg gcggtttcca cctccagcgc ccggcgcagg
     1381 aacatgttct cgcgcgcgtc ggcaatgcgg atgagggcca cgcgcgaacc gctctgcgga
     1441 atgacctcga cgagaccctc ggccgccagc cggcccatgg cttcagcgac cggaaagcgc
     1501 gagacgccga gcctcgcggc aattgcctgc ttgtccagcg gatcgccggg cttcaactcg
     1561 agcgaaacaa tggcttcccg tagcagggca tgggcccgcg ccgtcgcgcc ggtcttgccc
     1621 gtgggccggt cgggcgacag aaaatcatag gtgacggcga cagctgacat gctaacatgt
     1681 tagctggatt cctcgggctg gcaagccccg caacacgaaa gaccgcaaga tgatcgccac
     1741 cgtgcccaag accccccgca tgcttcgcct gacgcctgag gacaatgtcc tggtggccat
     1801 cgacgtggtc gaaaaggggg ccacggcgcc cgagggcatc gtggcgctgg agcgcatcat
     1861 gaagggccac aagatggccg cgacagagat cagggttggc cagccggtgc gcaagttcgg
     1921 ccagatcatc ggcttcgcca agcaggacat cccgaagggc gcctggatcc acgagcacaa
     1981 caccggcatg caggatttcg accgcgacta tgccttcgcc gaagaggcaa agcccgagga
     2041 catcctgccg ctcggccagc aggccacctt ccagggctac cgccgcgcca atggcaaggt
     2101 cggcacccgc aactacatcg gcatcatgac cagcgtgaac tgctctgcca ccgtggccgg
     2161 attcatcgcc gaagaggtga agcgctccgg catgctgaag gactatccca atatcgacgg
     2221 catcgtggcg ctgaagatgg acaatggctg cgtggtggat ttccgcggcg ccatcttcga
     2281 catccttaag cgcacggcct ggggctatgc caccaatccc aacatgggcg gcgtgctcat
     2341 ggtgggcctc ggctgcgagg gcttccagat cccgcgcttc aaggaagcct acaacgtgac
     2401 ggaaagcgaa accttccgga ccatgacgat ccaggaaacc ggcggtaccc gcaagacagt
     2461 ggccgcgggc gtggccgcgg tgcgcgagat gc
//
LOCUS       DBNGVR010000175         7583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3993737, whole genome shotgun sequence.
ACCESSION   DBNGVR010000175 DBNGVR010000000
VERSION     DBNGVR010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3993737"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="AC16A0_03730"
     CDS             <1..118
                     /locus_tag="AC16A0_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR3104471.1"
                     /translation="HHWLILHGRYLCKARKPECWRCPIADLCRFRPKTPAPA"
     gene            complement(90..752)
                     /locus_tag="AC16A0_03735"
     CDS             complement(90..752)
                     /locus_tag="AC16A0_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018917.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HIR3104472.1"
                     /translation="MMTAFFRALGDVLSPELRSVLWRALGLTLLLFIAVLVGVQMLLA
                     VLTEFSWPWADVVLGLGTGLVLLVAFFFLMAPVTAAFAGLFLDEVAARVELRHYPADL
                     PGTPLPAAAALFSGIRFAGLVLVVNLAMLPLVFTGIGAIALLLANAYLLSREFFEMAA
                     SRHMPLDEARRYRKENSPAVFLAGLVPAALALVPILNIAVPLFATAYFVHVFKLARAS
                     SA"
     gene            833..1825
                     /locus_tag="AC16A0_03740"
     CDS             833..1825
                     /locus_tag="AC16A0_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HIR3104473.1"
                     /translation="MTSHVDVLCIGNAIVDVLARVEDGFVNRHGLVKGSMNLIDEARA
                     ESLYADMGQAVEVSGGSAGNTAAGIASFGGKAAYFGKVKDDQLGAIYRHDMRAQGVSF
                     DSRASTEGPATARSFILVTPDGERTMNTYLGACVNLTVADIDSAVVESAKVTYMEGYL
                     WDRPEAKEAFKRAAAISRKAGRQTSITLSDSFCVERHRDSFLDLIRSGIDIVFANESE
                     IKSLYQTQNFDGAMQAIRKDCPIAVLTRSEAGCVVVRGEEVHAIPAYPVSKVVDVTGA
                     GDLFAAGFLHGLTHGLKLAHCAQLGALAAAEVISHIGARPEVNLRAHAKAVGLL"
     gene            complement(1820..2770)
                     /gene="coaA"
                     /locus_tag="AC16A0_03745"
     CDS             complement(1820..2770)
                     /gene="coaA"
                     /locus_tag="AC16A0_03745"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIR3104474.1"
                     /translation="MDELAKEVSPFHRFSREEWAARRADTPMTLIQADLDELRSLNDR
                     IDLTEVETIYLPLSRLLTLYVEATRGLFRATNHFLHLNDRKVPYIIGMAGSVAVGKST
                     TARILQRLLARWPRQPKVDLVTTDGFLLPNAVLEREGLMARKGFPESYDLPAILRFMS
                     DIKAGKRDVKAPVYSHLTYDVLKHEHVVVDQPDILIVEGLNVLQTGRPPRDGRGIPNT
                     SDFFDFSIYLDADPADLKTWYIERFLALRDGAFRHPKSYFNRYASLSDEEAVATATRI
                     WETINLVNLRENVLPTRPRADLVLRKGADHFIRSVFLRRL"
     gene            complement(2773..3105)
                     /locus_tag="AC16A0_03750"
     CDS             complement(2773..3105)
                     /locus_tag="AC16A0_03750"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR3104475.1"
                     /translation="MNNHRLEVLARLADTVAARKAAPAADSYTAKLLGKGVGACAKKL
                     GEEAVETALAAVSGDRRQIAAESADLLYHLVVVLAATGVDLAEVMAELERREGTSGLA
                     EKASRPKS"
     gene            complement(3117..3875)
                     /gene="hisF"
                     /locus_tag="AC16A0_03755"
     CDS             complement(3117..3875)
                     /gene="hisF"
                     /locus_tag="AC16A0_03755"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3104476.1"
                     /translation="MLKARLIPCLDVKDGRVVKGVNFVDLRDAGDPVEIAKAYDAAGA
                     DELCFLDITATHENRGIILDVVSRTAEACFMPLTVGGGVRRLEDIRRLLLAGADKVSI
                     NSAAVADRDFVRQAAEKFGSQCVVVAIDAKLVAPGGWEIFTHGGRKPTGIDAVDFARD
                     VVARGAGEILLTSMDRDGTGPGFDLELTRAIADAVPVPVIASGGVGTLDHLVAGIRDG
                     HAAAVLAASIFHFGTHSIAEAKRHLSSSGIPMRL"
     gene            complement(3877..4614)
                     /gene="hisA"
                     /locus_tag="AC16A0_03760"
     CDS             complement(3877..4614)
                     /gene="hisA"
                     /locus_tag="AC16A0_03760"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3104477.1"
                     /translation="MILFPAIDLKDGQCVRLKLGDMAQATVFNDDPAAQALAFQRQGF
                     SWLHLVDLNGAFAGQPVNGAAVEAILRAVTIPVQLGGGIRSMAQIESWLAHGIRRVIL
                     GTVALRDPQLVREACRSFPDRIVVGIDARGGRVAVEGWAETADITAIDLARRFADAGV
                     AAIIFTDIDRDGVLKGLNIDSTLELAAATTIPVIASGGLASIADVKRLLQPDCRILEG
                     AISGRALYDGRLDAGEALVLIRNAEAA"
     gene            complement(4611..5261)
                     /gene="hisH"
                     /locus_tag="AC16A0_03765"
     CDS             complement(4611..5261)
                     /gene="hisH"
                     /locus_tag="AC16A0_03765"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3104478.1"
                     /translation="MSVAIIDYGSGNLHSAAKAFERAARDSGLAARIDVTADPAVVRR
                     ASRIVLPGVGAFKDCRDGLKAVPGMWETLVEQVHGRGKPFLGICVGMQLMATRGLEHE
                     VTEGFGWIPGDVTPIEPRDASYKIPHMGWNTLDLVRPHALLEGIALGPQGLHAYFVHS
                     FHLACDQADDVIATTDHGCTITAIVGRDNVAGTQFHPEKSQALGLALIANFLGWSP"
     gene            complement(5266..5715)
                     /locus_tag="AC16A0_03770"
     CDS             complement(5266..5715)
                     /locus_tag="AC16A0_03770"
                     /inference="COORDINATES: protein motif:HMM:NF022400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HIR3104479.1"
                     /translation="MAIYSIFRKAGAPRDEAVFVKEGFSIAAFVLTFLWALWSRMWIV
                     AAVILAVMAAVAVAGSLTAANEVVVTAVNTGLALIFGFEAQSLRAWSLRRSGFVDDGL
                     VEAASREEAELKYFAAALPAERVVPRAAPVRSHGSSQSDPLGLFGNV"
     gene            complement(5715..6308)
                     /gene="hisB"
                     /locus_tag="AC16A0_03775"
     CDS             complement(5715..6308)
                     /gene="hisB"
                     /locus_tag="AC16A0_03775"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR3104480.1"
                     /translation="MRKASIDRKTTETDVTATVDLDGNGVFDISTGVGFLDHMLEQLS
                     RHSLIDISLKARGDVHIDFHHTTEDSGIALGQAVARALGDRKGIRRYASLHLPMDEAL
                     TRAAIDVSGRPYLIWKVAFNRPKIGDFDTELVREWFQAFAMNAGVTLHVETLYGDNNH
                     HIAESCFKALARCLREAIAIDPRQKNRIPSTKGSLQG"
     gene            6433..6981
                     /gene="hslV"
                     /locus_tag="AC16A0_03780"
     CDS             6433..6981
                     /gene="hslV"
                     /locus_tag="AC16A0_03780"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR3104481.1"
                     /translation="MAEQVQWHGTTILTVRKGGKVVIAGDGQVSLGQTVIKGNARKVR
                     PLGTGGHVIAGFAGATADAMTLFERLEAKLEQYPQQLTRACVDMAKDWRTDRYLRRLE
                     AMMIVADRSVSLVLTGTGDVLEPERGVIAIGSGGNYALAAARALIDTDMDAEAIARKA
                     MAIAAEICVYTNSNVTVESLDA"
     gene            6981..>7583
                     /locus_tag="AC16A0_03785"
     CDS             6981..>7583
                     /locus_tag="AC16A0_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104482.1"
                     /translation="MPGDAKQVIAGLESAIAAGAIDRATADRLTPYLLPVLQADPSAD
                     PDDERLRLVTGFNDIFVTIGLVMFLGALGYLLSSLGGALASLAVAGAAWGLAEFFTRI
                     RRMALPSIVLLLAFVASIFAAVPALFDGGSRSPFDIGLEPLPMMLAGVAAALAAWLHW
                     LRFHVPITVAAGVGALAVIVLALLARAAPDLVQTHPLPVFL"
BASE COUNT         1360 a         2473 c         2490 g         1260 t
ORIGIN      
        1 gcatcactgg ctgatcctgc atgggcgcta cctgtgcaag gcccggaagc cggaatgctg
       61 gcgttgcccc attgccgacc tctgccgctt caggccgaag acgcccgcgc cagcttgaag
      121 acgtgcacga agtaggccgt ggcgaagagc ggcacggcaa tgttgaggat cggcaccaag
      181 gccagggccg ccggcacaag gccggccaag aacaccgcgg gtgagttttc cttgcgatag
      241 cggcgggcct cgtccagcgg catgtggcgg ctggccgcca tctcgaagaa ctcgcggctc
      301 agcagatagg cattggccag cagcagcgca atggcgccga tgccggtgaa gacaagcggc
      361 agcatggcga ggttcaccac cagcacgagg cccgcaaaac ggatgccgga gaacagggcc
      421 gctgccgccg gcaggggcgt acccggcaga tcggcgggat agtggcgcag ctcgacacgg
      481 gccgccacct cgtcgagaaa cagtccggca aaggccgcag tcaccggggc catcaggaag
      541 aagaaggcca ccagcagcac aagcccggtg ccaagcccca gcaccacatc ggcccagggc
      601 caggaaaact ccgtcagcac cgccagcagc atctgcacgc ccaccaggac ggcgatgaac
      661 agcagcagcg tgagaccgag cgcccgccac agcacgctgc gcagttcggg cgacagcacg
      721 tcgcccaggg cccggaagaa agcggtcatc atggccctca cctaaggtgc aagccttgct
      781 agtgcaagcc cgctcggcta gggcaaggcc cgcagtcacc gcggagaatt tcatgaccag
      841 ccacgtcgat gtcctgtgca tcggaaacgc catcgtcgac gtgctggcgc gcgtcgagga
      901 cggcttcgtc aaccggcatg ggctggtcaa gggctccatg aacctgatcg acgaggcgcg
      961 cgccgaatcg ctctatgccg acatgggtca agccgtcgaa gtctccggcg ggtcggccgg
     1021 caacaccgcc gccggcattg cgtccttcgg cggcaaggcc gcctatttcg gcaaggtcaa
     1081 ggacgatcag ctgggcgcca tctaccgaca tgacatgcgg gcgcagggtg tgagcttcga
     1141 ctcgcgggcc tcgaccgagg gtcccgccac ggcccgctcc ttcatcctgg tgacgcccga
     1201 tggcgagcgc accatgaaca cctatcttgg cgcctgcgtg aacctgaccg tcgctgacat
     1261 cgactctgcc gtggtcgaat ccgccaaggt cacctacatg gaaggctatc tgtgggaccg
     1321 gcccgaggcc aaggaggcct tcaagcgtgc ggccgccatt tcgcgcaagg cggggcgcca
     1381 gacctccatc accctctccg attccttctg cgtggaacgc caccgcgaca gtttcctcga
     1441 cctgatccgg tcgggcatcg acatcgtctt cgccaacgag tcggagatca agtcgcttta
     1501 ccagacgcag aacttcgatg gcgccatgca ggccattcgc aaggattgcc ccattgccgt
     1561 tctcacacgc tcggaagccg gctgtgtcgt ggtgcggggt gaggaggtcc acgccattcc
     1621 cgcctacccg gtcagcaagg tggtggacgt gacgggggcg ggcgatctct tcgccgccgg
     1681 ctttcttcac ggcctcacgc atggtctgaa gctggcccat tgcgcgcagc tgggtgcgct
     1741 cgctgcagcc gaggtgattt cgcatatcgg cgccaggccc gaggtgaacc tcagggccca
     1801 tgccaaggcc gtgggcctgc tataggcggc gcaggaagac gctgcggatg aagtgatccg
     1861 cgcccttgcg caggaccaga tcggcgcgcg gccgcgtcgg cagcacgttc tcgcgcaggt
     1921 tcaccagatt gatggtctcc cagatgcgcg tggcggtggc gaccgcctct tcgtcgctga
     1981 gcgatgcgta gcggttgaag tatgacttcg gatggcggaa ggcgccgtcg cgcagcgcaa
     2041 ggaagcgctc gatgtaccag gttttcagat ccgccggatc ggcatcgaga tagatcgaga
     2101 agtcgaagaa gtcggaggtg ttgggaatgc cgcggccatc gcgcggcggg cggcccgtct
     2161 gcagcacgtt gaggccttcg acgatgagga tgtcgggctg gtccaccacc acatgttcgt
     2221 gcttcagcac gtcataggtg agatgcgaat agaccggcgc cttgacgtcg cgcttgcccg
     2281 ccttgatgtc ggacatgaag cgcaggatgg cggggagatc atagctttcc gggaagccct
     2341 tgcgggccat caggccctcg cgttcgagaa ccgcgttggg caggaggaat ccgtccgtgg
     2401 tgacgagatc gacctttggc tggcgcggcc agcgcgccag aagccgttgc aggatgcgtg
     2461 ccgtggtgct tttgcccacc gccacactgc cggccatgcc gatgatgtag ggcaccttgc
     2521 ggtcattgag gtggaggaaa tggttggtgg cgcggaacag gccgcgtgtc gcctcgacat
     2581 agagcgtcag caggcgcgac agcggcaggt agatggtctc cacctccgtc aggtcgatgc
     2641 ggtcgttgag gctgcgcagc tcgtcgaggt cggcctggat cagggtcatc ggcgtatcgg
     2701 cccgccgggc ggcccattcc tcgcgcgaga agcgatggaa gggcgagact tccttggcaa
     2761 gttcatccat gatcagctct tcgggcggct ggccttttcg gcaagcccgc tggtgccttc
     2821 gcggcgttcg agttccgcca tcacttcggc caggtcgacg ccggtcgccg ccagaacgac
     2881 gacgaggtga tagagcaggt cagccgactc ggcggcaatc tggcggcggt cgccggagac
     2941 ggccgcaagt gcggtctcga ccgcctcttc cccgagtttc ttggcgcagg ctccgacgcc
     3001 cttgcccagc agcttcgccg tatatgagtc agcagcgggc gcggcctttc gcgccgcgac
     3061 ggtgtcggcg agacgtgcca ggacctccag tcgatggttg ttcatgattt ctcctatcac
     3121 agccgcatcg ggatgccact ggacgaaaga tgccgcttgg cctcggcgat ggaatgggtt
     3181 ccgaaatgga agatcgatgc ggccagcacg gcggcggcat ggccatcgcg gatgcccgcg
     3241 accaggtgat cgagcgtgcc gacgccgccc gaggcgatca ccggaaccgg aaccgcatcg
     3301 gcaatggcgc gggtcagttc caggtcgaaa cccgggccgg tgccgtcgcg gtccatcgag
     3361 gtaagcagga tctcgccagc accgcgcgcc accacgtcgc gtgcgaaatc cactgcatcg
     3421 ataccggttg gcttgcggcc gccatgggtg aagatctccc atccgccggg tgcgaccagc
     3481 ttggcatcga tcgccacgac gacgcactgg ctgccgaatt tctccgcggc ctggcgcacg
     3541 aagtcgcggt cggccacggc cgccgagttg atcgacacct tgtcggcgcc ggccagcagg
     3601 agcctgcgga tgtcctcgag cctgcgtacc ccgccgccga ccgtcagcgg catgaagcag
     3661 gcttcggccg tgcggctcac cacatcgagg atgatgccgc ggttctcgtg ggtcgcggtg
     3721 atgtcgagaa aacaaagctc gtccgcaccc gccgcgtcat aggccttggc gatttccacg
     3781 ggatcgccgg catcccgcag gtcgacgaaa ttgacaccct tgacgacgcg gccgtccttc
     3841 acgtcgaggc aggggatgag gcgcgccttc agcatgtcat gccgcctccg cattgcggat
     3901 gagcaccagc gcctcgcccg catcgagccg gccatcatag agcgcgcggc ccgagatggc
     3961 gccttcgaga atcctgcagt cgggctgcag caggcgcttc acgtccgcga tcgaggccag
     4021 gccgccgctg gctatgacgg gaatggtcgt cgcggcagca agttccaggg tggagtcgat
     4081 attgagtccc ttcagaacgc catcgcgatc gatgtcggtg aagatgatgg cggcaacgcc
     4141 ggcatcggcg aagcgccgag ccaggtcgat ggcggtgata tccgccgttt cggcccagcc
     4201 ttcaaccgcg acgcgcccgc cgcgcgcatc gatgccgacc acgatgcgat ccggaaagct
     4261 ccggcaggcc tccctcacca gctgcggatc gcgcaacgcc acggtgccga gaatcacgcg
     4321 gcggatgccg tgcgcgagcc aggactcgat ctgcgccatg ctgcggatgc cgccgcccag
     4381 ctgcaccgga atcgtcacgg cccttaggat cgcctcgacg gcggcaccat tcaccggctg
     4441 gccggcgaag gcgccattga gatcgacgag atgcagccag gagaaaccct ggcgctgaaa
     4501 cgccagcgcc tgcgcggccg gatcgtcgtt gaacaccgtc gcctgcgcca tgtcacccag
     4561 cttcaggcgc acgcactggc catccttcaa gtcgattgca ggaaagagaa tcatggcgac
     4621 cacccgagga aattggcgat gagcgcaagg cccagcgcct ggctcttttc cggatggaac
     4681 tgcgtgcccg cgacattgtc acggcccacg atggcggtga tggtgcaacc gtgatcggtg
     4741 gtggcaatca catcgtcagc ctgatcgcag gcaagatgga aggaatgcac gaaataggca
     4801 tgcagccctt gcgggcccag ggcgatgcct tcaagcagcg catgcggccg gacgaggtcc
     4861 agcgtattcc atcccatgtg cggaatcttg tagctggcat cgcgcggttc gatcggtgtc
     4921 acgtcgccgg ggatccagcc gaaaccttcg gtcacctcgt gctccaggcc gcgcgtcgcc
     4981 atcagttgca tgccgacaca gatgccgagg aacggcttgc cccggccatg aacctgctcc
     5041 accagcgtct cccacatgcc gggcacggcc ttcagaccat cgcggcaatc cttgaaggcg
     5101 ccgacgccag gcagcacgat gcgcgaagcg cggcgaacca ctgcaggatc cgcagtcacg
     5161 tcgatccttg cagcgaggcc cgaatcccgg gccgcgcgtt cgaaggcctt ggcggcggaa
     5221 tgcagattgc cggagccgta gtcgatgatg gcgacgctca tcgggtcaca cgttcccgaa
     5281 gaggcccagc ggatcgctct ggctgctgcc atggctgcgg accggcgccg cccgcggaac
     5341 aacgcgttcg gccggcaatg ctgccgcgaa atacttcaac tccgcttctt cgcgactcgc
     5401 ggcctcgacc agcccatcat ccacgaagcc cgaacggcgc agcgaccagg cccgcaagct
     5461 ctgcgcttcg aagccgaaga tcagggcgag acccgtgttg acggcggtca ccaccacctc
     5521 atttgctgcg gtcagcgatc ctgcaacggc aacggccgcc atcaccgcca gaatcacggc
     5581 ggccacaatc cacatgcggc tccacagggc ccagaggaag gtcagcacaa atgccgcgat
     5641 ggagaatccc tccttcacga aaacggcctc gtcgcgcggc gcgccagcct tgcggaatat
     5701 ggagtagatc gccatcagcc ctgcagcgat cccttggtcg agggaatgcg gttcttctgg
     5761 cgcggatcga tcgcgatggc ctcgcgcagg cagcgcgcca gcgccttgaa gcaggactcc
     5821 gcgatgtgat ggttgttgtc gccatagagc gtttcgacat gcagcgtgac gcccgcattc
     5881 atggcaaagg cctggaacca ctcgcgcacc agctcggtat cgaagtcgcc gatcttgggg
     5941 cggttgaacg caaccttcca gatcagatag ggacggcccg agacatcgat ggccgcccgg
     6001 gtcagcgcct catccatggg aagatgcagg ctggcgtagc ggcggatacc cttgcggtcg
     6061 cccagcgcac gggccacggc ctggcccagc gcaatgccgg aatcctccgt tgtgtgatga
     6121 aagtcgatat ggacatcgcc cctggccttg aggctgatgt cgatgagcga atggcgcgac
     6181 aattgctcca gcatgtgatc gaggaatccg accccggtcg agatgtcaaa gacgccattg
     6241 ccgtcgaggt cgacggtggc cgtcacatcc gtttcggtgg tcttgcggtc gatactggct
     6301 ttacgcatgg aactccctcc gctgcccggc ggtcccggcc ctttagcaga agcgccggga
     6361 aatggccaag ggtggctttt ctttctgcag gccaaggcct acatgaagcg ttaacggaac
     6421 aggacatgag ccatggcaga gcaggtacag tggcatggga cgacgatcct caccgtccgc
     6481 aagggcggca aggtggtgat cgcaggtgac ggccaggtga gcctcggcca gacggtgatc
     6541 aagggcaatg cgcgcaaggt gcggccattg ggaacgggcg gccacgtcat cgcaggcttc
     6601 gccggcgcca ctgccgatgc catgacgctc ttcgagcgcc tcgaggcaaa gctcgagcaa
     6661 tatccgcagc agttgacccg ggcctgcgtc gacatggcca aggactggcg caccgaccgc
     6721 tatctgcgcc gcctcgaggc catgatgatc gtggccgacc gcagcgtcag cctggtgctg
     6781 accggcaccg gcgacgtgct ggaacccgag cgcggcgtca ttgcaatcgg ttcgggcggc
     6841 aactacgccc tcgcggccgc ccgcgcgctg atcgataccg acatggacgc agaggcaatt
     6901 gcccgcaagg ccatggcgat cgctgccgag atctgcgtct acaccaattc caacgtcacg
     6961 gtcgaaagcc tggacgcgtg atgccgggcg atgccaagca ggtgatcgcg ggcctcgagt
     7021 cggccatcgc cgccggggcc atcgaccggg ccacggcgga ccggctgacg ccctatctcc
     7081 tgcccgtcct gcaggcagat ccttccgccg atccggatga cgaacggctg cgtcttgtta
     7141 cgggattcaa cgacatcttc gtgaccatcg ggctggtgat gtttttgggc gcgctgggat
     7201 atcttctctc cagcctgggt ggtgcgcttg cgtcgctggc ggttgccggc gcggcctggg
     7261 gcctcgctga attcttcacg agaatccggc gcatggcgct gcccagcatc gtgctgctgc
     7321 tggccttcgt tgcctcgatc tttgccgccg tgcccgccct gttcgacggc ggatcgcggt
     7381 cgcccttcga catcggtttg gagccgctgc cgatgatgct ggccggcgtg gcagccgcgc
     7441 tggctgcctg gctgcactgg ctgcgcttcc acgtgccgat cacggtggcc gccggcgtcg
     7501 gcgctcttgc cgtgatcgtg ctggccttgc tggcaagggc agcgcccgac cttgtgcaga
     7561 cccatccgct tcccgtcttc ttg
//
LOCUS       DBNGVR010000176         2101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2226188, whole genome shotgun sequence.
ACCESSION   DBNGVR010000176 DBNGVR010000000
VERSION     DBNGVR010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2226188"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="AC16A0_03790"
     CDS             complement(<1..1355)
                     /locus_tag="AC16A0_03790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3104483.1"
                     /translation="MRPSILNPLFAGAAGIKGIGSKLDRLLAGFLRPSHGAAGETTRV
                     LDLLFHLPSGIVDRRFRPRIADLPREGVVTVEVTVGKHRPPPAFNKRVPYRIDVHDQT
                     GTLTLVFFHSYADSLKRMLPEGERRFVSGKIDWYGAEPQMAHPDHVVSAEEFDRLPLI
                     EPIYPLTEGVSGKILTKAIRAAIERLPVLPEWQDDAFRQRHAWPAFDAALRALHHPET
                     PAALGPEAPSRRRLAYDELLANQLALALVRANLKRAAGRTLRGTGAIREKILAALPYM
                     LTDGQRHAVDDILRDMAAPERMLRLLQGDVGAGKTVVALLALATAVESGAQGALMAPT
                     EILARQHLATLSKLAATSGLRIALLTGREKGAARDDVLARAAAGEIDILIGTHALFQE
                     HVVFKDLGLVVIDEQHRFGVHQRLALQAKAGSLADLLVMTATPIPRTLALTVYGDMDV
                     S"
     gene            1418..1684
                     /locus_tag="AC16A0_03795"
     CDS             1418..1684
                     /locus_tag="AC16A0_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015870.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006105 - succinate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0034552 - respiratory chain complex II
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HIR3104484.1"
                     /translation="MTPASDSDEIRRKRLLWRATHRGIKEMDIIFGGFVQRGLTRFTP
                     AELDELERMVALPDQELLAWATQMAPVPSAHDGPLLRAMLAYRP"
     gene            1681..>2101
                     /locus_tag="AC16A0_03800"
     CDS             1681..>2101
                     /locus_tag="AC16A0_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3104485.1"
                     /translation="MIDLSRLKSRLGVSGRVQVSGVPQGLDAMILPEVAKAIGSRAIV
                     HVALDDQRAAVLADQLAFLAPELEVLRFPAWDCLPYDRVSPVPDIVARRLATLARLAQ
                     PLSKPTVVLATVNAVLQRVVPRGLIAAATFSAAPGNRV"
BASE COUNT          337 a          654 c          764 g          346 t
ORIGIN      
        1 ttcgagacat ccatgtcgcc atagacggtc agcgcaaggg ttcggggaat tggcgttgcc
       61 gtcatgacca ggagatcggc gaggcttccg gccttggcct gcagcgcaag ccgctgatgc
      121 acgccgaagc ggtgctgctc gtcgatgacc acgaggccca gatccttgaa gacgacgtgt
      181 tcctggaaca gcgcatgcgt gccgatgaga atgtcgatct cgccagccgc cgcgcgtgcg
      241 aggacatcat cgcgggcggc gcccttctcg cgcccggtga gcagggcgat gcggaggccg
      301 gatgtggccg cgagtttcga cagggtcgcc agatgctgcc gcgccaggat ttcggtgggc
      361 gccatcagcg ccccctgagc gccgctctcg acggctgttg cgagggcaag caacgcgacc
      421 acggtctttc ctgcgccgac atcgccctgc aagaggcgca gcatccgctc gggcgctgcc
      481 atgtcgcgca ggatgtcgtc cacggcgtgg cgctgcccat cggtgagcat gtagggaagg
      541 gccgcgagga tcttctcgcg gattgcaccc gttccgcgca gcgtgcgtcc tgcggcgcgc
      601 ttcagattgg cgcggaccaa ggcgagggca agctggttgg cgagcaactc gtcgtacgca
      661 aggcggcggc gggatggggc ctcgggacca agggcggccg gggtctcggg atggtgcagg
      721 gcgcgcagtg cagcatcgaa ggcaggccag gcgtggcgct ggcggaaggc atcgtcttgc
      781 cactccggca gaaccggcaa gcgctcgatc gcggcacgga tggccttggt caggatcttt
      841 cccgaaacgc cttcggtcaa aggatagatc ggctcgatga gcgggagcct gtcgaactcc
      901 tcggccgaga cgacgtgatc gggatgggcc atctgcggct ctgctccata ccagtcgatc
      961 ttgccggaca cgaagcgccg ttcgccctcg ggcagcatgc gcttcaggct gtcggcgtag
     1021 ctgtggaaga acaccagcgt gagggttccg gtctggtcgt ggacatcgat gcgatagggg
     1081 acgcgtttgt tgaacgccgg cggcggcctg tgcttgccga cggtcacctc gacggtgacc
     1141 acaccctcgc ggggaagatc ggcgatgcgc ggccggaaac ggcggtcgac gattccggag
     1201 gggaggtgga agagaagatc gaggacgcgc gttgtctcgc ctgcggcgcc atgcgatggc
     1261 cgcaggaaac cggccagcag cctgtcgagc ttgctgccga tgcccttgat gccggcggcg
     1321 ccggcaaaga gcgggttgag gatcgaagga cgcatggcag cagtgtcggg gggaacgggt
     1381 tgcggcgcaa gggtggatga ggcaaagagc accgcaaatg acccccgcca gcgacagtga
     1441 cgagatccga cgcaagcgcc tgctgtggcg ggcgacccac cggggaatca aggaaatgga
     1501 tatcatcttc ggcggcttcg tgcagcgcgg actgacccgg ttcacgccgg ccgagctgga
     1561 cgagctggaa cgcatggtcg cgttgccgga ccaggaactg ctggcctggg caacgcagat
     1621 ggcgccagta ccttcggcgc atgacgggcc cctgctgcgc gccatgctgg cgtatcggcc
     1681 atgatcgacc tttcccgcct gaagtcgcgg ttgggggtgt ccggtcgcgt gcaggtctcc
     1741 ggcgtgccgc aaggcctgga cgccatgatc ctgccggagg tggcgaaggc gatcggctca
     1801 cgcgccatcg tgcatgtggc gctcgacgat cagcgcgctg ccgtgctggc cgaccagctg
     1861 gccttccttg cgcctgagct tgaggtgctt cgtttcccgg cctgggattg cctgccctat
     1921 gaccgcgtct cgccggttcc cgacatcgtg gcccgccggc ttgcgacgct ggcgcggctg
     1981 gcacagccgc tgagcaagcc gacagtggtg ctcgccacgg tgaatgcggt tctgcagcgc
     2041 gtcgtgccgc gcgggctgat cgccgccgcg accttctccg ctgcaccggg caaccgcgtc
     2101 c
//
LOCUS       DBNGVR010000177         4835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_853419, whole genome shotgun sequence.
ACCESSION   DBNGVR010000177 DBNGVR010000000
VERSION     DBNGVR010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_853419"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC16A0_03805"
     CDS             <1..263
                     /locus_tag="AC16A0_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3104486.1"
                     /translation="TKSHPSKVIASDTFVGYCGGKWILLGTEKLRGFEQAVKIFAPDT
                     ARVKGRGLELTQGQEEAGMSDAEHLMMLHRQAGQAAQKSAGR"
     gene            269..1216
                     /locus_tag="AC16A0_03810"
     CDS             269..1216
                     /locus_tag="AC16A0_03810"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 20 protein"
                     /protein_id="HIR3104487.1"
                     /translation="MQHASPDTALTIVTRAAPDLRYDDLLVDGFDGEGFSPAGGLYYK
                     PTFEQSAGSVEFQRDVSLDGRGALKLSVKPLGSGGKRTDISERAEIWERPQLRVPYDR
                     GIWYGFAVKFADPVPQDDHRYLIAQWKREIDPGHPLDFSPFLALRLNKGKLFATVETN
                     YHPPIPSLGGRADGVDAGRVPVKLRPETNQMRALIATPSNWTPADGELFHTSSDKLTV
                     IDRGTPMPDPRSGWIDFVVYTKPGPDGSGHIEIFANGKWIVTVKGHIGHNDDGLGQNQ
                     YFKFGPYRAGHSTVWTLYYDDFRRSPRCADVLRGIACPF"
     gene            1379..3277
                     /locus_tag="AC16A0_03815"
     CDS             1379..3277
                     /locus_tag="AC16A0_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104488.1"
                     /translation="MKPVNDLLRIEDLGVGITMQSGQLEAVKSASLRVLPGKVTALVG
                     ESGSGKSVIAQTILGILPPVAKVTKGRILFNDPERPGSSIDLCQLDRDSEAYRALRGQ
                     RISMIFQEPMTALSPLHTVGNQIQEALTIHTSLPEAEARRRTEEMLDLVGFPDPKRAY
                     GMYPFELSGGLRQRAVIAMALICRPALVVADEPTTALDVTIQAQILQLLRELQSKLNM
                     AILLITHDLGIVANVADEVVIIYHGEIMEAGTVSDIFRNPQHPYLKGLLRAVPHFDMA
                     PGERLKSLRDVQVDTSKLKQAETRTAGKRGSILLSAKGVTKSFSTRKSGWFAKQESTA
                     RKALDDVSLEIRRGECLGLVGESGCGKTTLSKVIMRAVRPDAGTVTYDDGNGPIDVLA
                     AEGEDLQTLRTKIQMVFQDPFSSLSPRMTVQNILSEPLEIHNRGDAKSRIEQVKTLME
                     VVGLDPRFLSRYPHSFSGGQRQRVGIARALALGPDLLICDEPVSALDVSVQAQILNLL
                     KDLQAQLGLTYLFISHNLAVVDYMADRIAVMCRGRIVEIAPREVLFKSPIHPYTRALL
                     AAVPFPDLDRPLDYTTLQLGTASDTGTWPSFFREADNKAPLVAAHCGEDHFVLAQASA
                     KVQELRAW"
     gene            3271..>4835
                     /locus_tag="AC16A0_03820"
     CDS             3271..>4835
                     /locus_tag="AC16A0_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015008336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104489.1"
                     /translation="MVTRRTTLGLLASVALARPTRADDLEPPFLRERLSSGELPPVAE
                     RLPKVPRVINLEAMGRVPGRHGGDIRTLIGGQRDIRLMTLYGYSRLVGYDEKLVLQPD
                     ILERYDIVEDRIFTFRIREGHKWSDGSPFTAEDFRYAWEDIQLNEDLSPGGLSTYLLS
                     NGKPPVFEIIDPLTVRYSWESPNPDFLPRIAAAQPLNMAMPAAYLKQFHKKYQTEEKL
                     AELVKKNKVKKWTVLHTRMSRTYRPENPDLPTLDPWRNTTAPPAEQFIFERNPYFHRV
                     DEAGRQLPYVDRFILNSSTSSIIPAKTGAGESDLQANAIDFTDYAFLKESEQRYPVKV
                     TLWKKTQASRLALLPNLNYKDPAWQALFRDPRVRRALSLAIDRHEINEALFFGLGTES
                     SDTLMPESPLFKPEFAKAWIEHDPEQANALLDAAGLSARDSDGIRLLPDGRRAEIIIE
                     TAGESTLETDALELVTDYWAKVGVKLFIRTSQRDIFRSRTIGGEIMMAIWSGMDNGIA
                     TGDMNPAQLAPTS"
BASE COUNT          955 a         1504 c         1465 g          911 t
ORIGIN      
        1 tgaccaagag ccacccgtcc aaggtcattg ccagcgatac ctttgttggc tattgcggcg
       61 gaaagtggat cttgctgggg accgagaagt tgcgcgggtt cgagcaggcc gtgaagatct
      121 tcgcaccgga cacggcccgg gtgaagggca ggggtctgga attgactcag ggacaggaag
      181 aggctggaat gtctgacgcc gaacatctga tgatgcttca ccggcaggct ggccaggcgg
      241 cacagaagtc cgccggacgc tgaggttctt gcaacatgcg tcccccgata cggccctgac
      301 catcgtgacc cgtgcagcac cggatctgag gtacgacgac ctgctggtcg acgggttcga
      361 cggggagggt ttttcgcccg ccggcgggct ctattacaag ccgacctttg aacagagcgc
      421 cggctctgtc gaattccaga gggacgtctc gctcgatggg cgtggtgcgc tcaagctctc
      481 ggtgaagccg ctcggaagcg gcggcaagcg caccgacatc agcgagcggg ccgaaatctg
      541 ggaaaggcca cagctgcgtg tgccctatga caggggcatc tggtatggtt ttgcggtgaa
      601 attcgccgat ccggttccgc aggacgatca ccgctatctg attgcccaat ggaagcgcga
      661 gatcgacccg ggccaccccc tggacttcag ccccttcctt gccctgcggc tgaacaaggg
      721 taagctcttc gccacggtgg aaacaaacta ccacccgccc atcccgtccc tcggcggcag
      781 ggcagatggc gtcgacgccg ggcgcgtgcc ggtaaagttg cggccggaaa ccaaccagat
      841 gcgcgccctg atcgcgacgc cgtccaactg gacgcctgcc gacggcgagc tgtttcatac
      901 cagttcggac aagctcacgg tcatcgaccg tggcacgccc atgccggatc cccgctcggg
      961 ctggatcgat tttgtcgtct ataccaagcc tggacccgat ggctcgggcc atatcgagat
     1021 tttcgccaat gggaagtgga ttgtcaccgt gaaggggcat atcggccaca acgatgacgg
     1081 actgggacag aatcagtatt tcaagttcgg accctaccgg gccgggcaca gtaccgtgtg
     1141 gacgctctac tacgatgact ttcggcgctc gccccgctgc gccgacgtgc tgcgcggaat
     1201 agcctgtcct ttctgagtca tacggcgttc atgagatcgt gatttcgctt gcctttccag
     1261 tgacggggga gatgaggcgg ggtgtgtgaa caggaatgtg gtgcccctgt gttttcccga
     1321 ttgacaaagt gacaggagaa caccgacgat cccctgtaag tcaccttgat ggcaaagaat
     1381 gaaacctgtg aacgaccttc tgcggatcga ggaccttggt gtcggcatca ccatgcagtc
     1441 cggacaactt gaggctgtaa agagcgcctc gcttcgcgtt cttcccggca aggttacagc
     1501 cctcgtgggg gaatccggct caggaaaatc agtgattgcc cagaccattc tgggaattct
     1561 gccgcctgta gccaaggtta ccaaggggcg gatcctcttc aacgatccgg aaaggccggg
     1621 cagcagcatc gacctgtgcc agctcgaccg cgacagcgaa gcctaccggg cgctgcgcgg
     1681 tcagcgcatt tcgatgatct ttcaggaacc gatgaccgcg ctttcgccgc tgcatacggt
     1741 tggcaaccag atccaggaag ccctgaccat tcacaccagc ctgccggagg cggaggcgcg
     1801 gcggcggact gaggagatgc ttgatctcgt cggctttccc gatcccaagc gcgcctatgg
     1861 catgtatcct ttcgaattgt ccggcggcct gcggcagcgg gcggtgatcg ccatggcgct
     1921 gatctgccgc ccggccctgg tggtggccga cgaacccacc actgccctgg acgtgacgat
     1981 ccaggcgcag atcctgcagc tgctgcgcga gttgcagtcg aagctcaaca tggccatcct
     2041 cctgatcacc catgatctgg gcatcgtcgc caatgtggct gacgaggtgg tgatcatcta
     2101 tcacggcgag atcatggagg cggggactgt ctccgacatc ttccgaaatc cccagcaccc
     2161 ctatctcaag ggcttgctgc gggccgtgcc gcatttcgac atggcccccg gcgagcgcct
     2221 gaaatcgctt cgcgacgtgc aggtggatac ctcgaagctg aagcaggccg aaacccgaac
     2281 ggcagggaaa cgcggttcga tcctgctctc cgccaagggg gtgaccaagt cgttctccac
     2341 gcgaaagtcc ggctggtttg caaagcagga aagcactgcg cgcaaggcgc tggacgacgt
     2401 gagcctcgag atccgccgcg gcgaatgcct gggtctggtc ggcgaaagcg gatgtggcaa
     2461 aacaacactg agcaaggtca tcatgcgcgc agtaaggccc gatgccggca ctgtgaccta
     2521 tgacgacggc aacggaccga tcgatgtgct ggccgccgag ggagaagacc tgcagacgct
     2581 tcgcaccaag atccagatgg tattccagga cccgttctcg tcgctgtcgc cgcgcatgac
     2641 ggttcaaaac attctttccg agccattgga gatccacaat cgcggcgacg ccaagtcgag
     2701 gattgaacag gtcaagacct tgatggaagt ggtcggtctc gatccgcgct tcctgagccg
     2761 ctatcctcac agcttttccg gtggccagcg ccagcgtgtc ggcatcgccc gtgccttggc
     2821 gcttgggccc gatctcctca tttgcgacga accggtttcg gcgctcgatg tctcggttca
     2881 ggcccagatc ctgaacctgc tgaaggatct tcaggcccag ctgggcctta cctatctctt
     2941 catttcccac aatctggccg tggtcgacta catggccgac cggatcgccg tgatgtgccg
     3001 cggcaggatc gtggagattg cgccgcgcga ggtgctgttc aagtcgccaa tacatcccta
     3061 tacgcgtgcc ctgctggcgg cagtgccatt cccggatctc gaccgcccgc tcgactatac
     3121 cacgctgcag ctcggcacgg cgtccgatac cggcacatgg ccgtccttct tccgggaggc
     3181 agacaacaag gcgccgctgg tggcggcgca ttgcggtgaa gatcacttcg tgctggccca
     3241 ggcttcagcc aaggtgcagg agttacgcgc atggtaacgc gccggaccac tctcggactg
     3301 cttgcatcgg tcgccttggc gaggcccacg cgcgccgacg acctcgaacc gccattcctt
     3361 cgtgagcggc tgtcgagcgg cgaacttccg cctgtggccg agcgcctgcc caaagtgccg
     3421 cgcgtcatca atctcgaagc catgggccgg gttccgggga ggcatggcgg cgatatccgc
     3481 accctcatcg gagggcagag ggatatccgc ctgatgacgt tgtacggcta ttcgcggctc
     3541 gtcggctacg acgagaagct tgtccttcag cctgacatcc tggagcgcta tgacatcgtc
     3601 gaggacagga ttttcacctt caggatccgc gagggacaca agtggtcgga cggctcgccc
     3661 ttcaccgcag aggatttccg ttacgcttgg gaagatatcc agctcaacga ggacctgtcg
     3721 cccggcggcc tctccacata tcttctttcc aacggcaaac cgcccgtctt cgagatcatc
     3781 gatccgctga ccgtccgcta tagctgggaa tcgcccaatc cggatttcct gccgcgcatc
     3841 gcagcggcgc agcctctgaa catggcgatg ccggctgcct acctcaagca gttccacaag
     3901 aaataccaga cggaggagaa gctcgccgaa ctggtgaaga aaaacaaagt gaagaaatgg
     3961 accgtgctgc atacgcgcat gtcccggacc tatcggccgg agaacccaga tctacccacg
     4021 cttgatccgt ggcgcaacac gaccgcgccg ccggccgagc agttcatttt cgagcgcaac
     4081 ccgtatttcc atcgcgtcga tgaggcgggg cgccagcttc cctatgtcga ccgcttcatt
     4141 ctcaacagca gcacgtcgtc gatcattccg gccaagaccg gtgcgggaga atccgacctg
     4201 caggcaaatg ccatcgactt caccgattat gccttcctga aggaatccga gcagcgctat
     4261 ccggttaagg tcacgctttg gaagaagact caggcttcgc gcctcgccct gcttcccaac
     4321 ctcaattaca aggatccggc atggcaggcg ctgttccgcg atccgcgggt gcgtcgtgcc
     4381 ctgtcgcttg ccatcgatcg ccacgagatc aacgaagcac tgttcttcgg tctcggcacc
     4441 gagagttccg acaccctcat gccggagagc ccgctgttca agcccgagtt cgcaaaggcc
     4501 tggatcgagc atgatccgga acaggccaat gcgttgctgg atgcggccgg attgtcggcg
     4561 cgtgacagcg acggaatcag gctgctgccc gatggccgcc gggccgaaat catcatcgag
     4621 accgccggcg aaagcacgct cgagaccgac gcgctcgaac ttgtcaccga ctattgggcg
     4681 aaggtcggag tcaagctctt catccgcacg tcgcagcgcg acatcttccg cagccgcacc
     4741 atcggcggcg aaatcatgat ggctatctgg tcgggcatgg acaatggcat agcgaccggc
     4801 gacatgaatc cggcgcagct ggcgccgaca tcgga
//
LOCUS       DBNGVR010000178         6689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3405336, whole genome shotgun sequence.
ACCESSION   DBNGVR010000178 DBNGVR010000000
VERSION     DBNGVR010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3405336"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1334)
                     /locus_tag="AC16A0_03825"
     CDS             complement(78..1334)
                     /locus_tag="AC16A0_03825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3104490.1"
                     /translation="MYGLLDRAFKAVLAQGTMRVTGPDGVTRSYGDGTGSPVHLLIKT
                     TEAARKLVFDADLFLGECYMDGSIDVKDCTIYDVLALLMANVEANKMPAPARLMYGLR
                     RFKKRIDQYNPVGKAKENVAHHYDLSGALYDLFLDRDRQYSCAYFEGPDASLEDAQLA
                     KKRHLAAKLMIKPGMKVLDIGSGWGGLGLYLAETCGAEVTGVTLSEEQHKLSNERAEQ
                     RGIADRVKFHLKDYRHLDQRFDRIVSVGMFEHVGVGHFAEYFGKIRSLLADDGAAVVH
                     SINRSDGPGATSAWVKKYIFPGGYIPALSEVVPQIEGAGLYITDIEILRLHYAETLRE
                     WAHRFADHRDRAREIYDERFCRMWEFYLAASECAFRFGGMNNFQIQFCRNQNALPLTR
                     NYMIKEEERLRAIDSKRQRLKSIPAE"
     gene            complement(1544..2110)
                     /gene="rplI"
                     /locus_tag="AC16A0_03830"
     CDS             complement(1544..2110)
                     /gene="rplI"
                     /locus_tag="AC16A0_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004691968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3104491.1"
                     /translation="MDVILLERVAKLGAMGEVVKVKDGYARNFLLRRGKALRATDANK
                     AKFERDRADLEARNADRRGAAEAEAKGLAGKTFVLIRQAGESGQLYGSVTPRDIAEAA
                     TATGVKVERGHVHLDAPIKTIGLYTVSVIPHPEVEVKVTVNVARSADEADAQARGEDL
                     TGPVSDRAEIRAAAEALFEQEAEAQDNA"
     gene            complement(2124..2366)
                     /gene="rpsR"
                     /locus_tag="AC16A0_03835"
     CDS             complement(2124..2366)
                     /gene="rpsR"
                     /locus_tag="AC16A0_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3104492.1"
                     /translation="MSTGQPARRPFFRRKKSCPFTGDNAPKIDFKDTRLLQRYISERG
                     KIVPSRITAVCAKKQRELAKAIKRARFLGLLPYVLK"
     gene            complement(2366..2815)
                     /gene="rpsF"
                     /locus_tag="AC16A0_03840"
     CDS             complement(2366..2815)
                     /gene="rpsF"
                     /locus_tag="AC16A0_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3104493.1"
                     /translation="MALYEHVFLTRQDASNTQVDALTEQFKGLIAAGGGKVTKTEYWG
                     VKSLSFRIKKNRKAHYTLMNIDAPAAAVAEMERQMSLSEDVIRHLTVKVEALEEGPSA
                     QMRRRDDREERSDRGFGGGGGFGGREGRESRDGGRGRREEAEVEGAN"
     gene            3078..3470
                     /locus_tag="AC16A0_03845"
     CDS             3078..3470
                     /locus_tag="AC16A0_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104494.1"
                     /translation="MVPRMSCRGTGQKRAARAGVALSLLFAFLLPLLLGVLPAPTAAA
                     QLAQDIAASYCDPNGERPAQSPHGDHKTCCILCPVGGLAALEARDVPIDPAPAAQADV
                     WVAGVGLPLLPGRRIDLAQIVPRGPPSP"
     gene            3543..4943
                     /locus_tag="AC16A0_03850"
     CDS             3543..4943
                     /locus_tag="AC16A0_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HIR3104495.1"
                     /translation="MSSTTLPQQDAVASTRLYRAMWRWHFYAGLYVIPFLIMLAVTGL
                     IILWFTAIAPEYGDRLTLQPGAQALSITQQADAALAAHPGGTVGKYVPPLVPENPALF
                     QVNLEQGNRMIAIDPYTGAILRDRAQDGTWNEFATDIHGSLLLGGSGGFGDLLIEVAA
                     SLGIVLLITGLYLWWPRGTGWRDVLVPRLSAGGRAFWKSIHSVTGFWMSLVILFFLVS
                     GLSWAGVWGGKFVQAWSTFPAEKWDSVPLSDEALAGMNHGALKEVPWALEQTLMPASG
                     SAAGVTGLPEGVPVVLESVVALGRAIGFEGRFQVAVPADKTGVWTLSQDSMSYDSPDP
                     TADRTVHVDQYTGKILASAAFADYSLAGKAMAVGIALHEGQMGLWNIVLNVVFCLAVI
                     LIAVSGVVMWWLRRPVGQFGVPLYPKAYRVPVAILGLAVLVSIAFPLGGAAIALIAVV
                     DFLLPKRVKEAGSAKA"
     gene            4971..5444
                     /locus_tag="AC16A0_03855"
     CDS             4971..5444
                     /locus_tag="AC16A0_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR3104496.1"
                     /translation="MKLFKELAFAATLSLASIYAIASGHAHEIKVGDIVIAHPWSRPA
                     PMASDVAAGFMKITNTGTADDRLVSATATITGTVQLHDMKMEGDVMKMFEIPGGIVIP
                     AGQTVELKPKSLHVMFMDLKRAPVEGEAFKGTLTFEKAGTVEIDYEVTAQAPEAH"
     gene            5444..6028
                     /locus_tag="AC16A0_03860"
     CDS             5444..6028
                     /locus_tag="AC16A0_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3104497.1"
                     /translation="MLRFIRIASLVTAALLAAVTGYVYLTRGEAVVSVADIGGPFRLA
                     SASGGVVDSQDLAGKPYAVFFGFTRCPEVCPTTMAEMSAALTELGDEGKDLRVFFVTV
                     DPERDTAEFLKDYLSSFDPRIEALVPTAEELAAMARSFRAYYAKSPTSDGGYTMDHTA
                     TVFLMNRQGKLASTISYGEEQATRMAKLRKLLGV"
     gene            6086..>6689
                     /locus_tag="AC16A0_03865"
     CDS             6086..>6689
                     /locus_tag="AC16A0_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104498.1"
                     /translation="MRSKSLSVILFVLSLAEARAEDGVGLAPAVAAIFPRGEEVCYLA
                     GPETTGLKSGQKLSEFYLYRFYDPLHAREEIGPSREEAIARERKINSSYASLDVVARF
                     RDSRNVYSQSVLCTTYDGGKTITCGVECDGGSFAVEPGNRALQITVPADGSGLSLNQS
                     CGDPDDEGRERWLTGADAGGSFTLERWPMAHCIAAAEDAHP"
BASE COUNT         1073 a         2180 c         2183 g         1253 t
ORIGIN      
        1 atgggaccgt gttagccgat gaatcggatc agtggcgcag tttgcgcgct tatacccgtt
       61 atccacaagg gcggcgacta ctccgcaggg atcgatttca ggcgctgccg cttcgagtcg
      121 atggcccgca ggcgttcttc ctctttgatc atgtagttgc gcgtcagcgg cagggcgttc
      181 tggttgcggc agaactggat ctggaaattg ttcatgccac cgaagcggaa ggcgcattcc
      241 gacgcggcga gatagaactc ccacatgcgg cagaagcgct cgtcgtagat ctccctggcg
      301 cggtcgcggt ggtcggcgaa gcggtgggcc cattcgcgca gcgtctcggc atagtgcagg
      361 cgcaggatct cgatgtcggt gatgtagagg ccggcaccct cgatctgcgg cacgacttcc
      421 gacagggcag ggatatagcc gcccgggaag atgtacttct tgacccatgc gctggtggcg
      481 cccggcccgt ccgagcggtt gatggaatgc accacggcag caccatcgtc ggcgagcagc
      541 gagcggatct tgccgaagta ttcggcgaaa tggccgacgc cgacatgctc gaacatcccg
      601 accgagacga tgcggtcgaa acgctggtcg aggtggcggt agtccttgag gtggaacttc
      661 acgcggtccg cgatgccgcg ctgttcggcg cgctcgttgg acagcttgtg ctgttcttcc
      721 gacagggtca cgccggtcac ctcggcgccg caggtctcgg ccagatagag gcccagcccg
      781 ccccagcccg agccgatgtc cagcaccttc atgccgggct tgatcatcaa cttggcggcg
      841 aggtggcgct tcttggccaa ctgcgcatct tcgagcgatg cgtccgggcc ttcgaaatag
      901 gcacaggaat actgccggtc gcggtcgagg aagagatcgt agagcgcgcc cgagagatcg
      961 tagtggtgcg ccacgttctc cttggccttg ccaaccggat tgtactggtc gatgcgcttc
     1021 ttgaagcggc gcaggccata catgaggcgg gcgggggccg gcatcttgtt ggcctcgaca
     1081 ttggccatca gcagcgccag cacgtcatag atcgtgcagt ccttcacgtc gatgctgccg
     1141 tccatgtagc attcgccaag aaagaggtcg gcgtcgaaga caagcttgcg cgccgcctcc
     1201 gtggtcttga tcagcagatg caccggtgac cccgtgccgt cgccatagct gcgcgtgacg
     1261 ccatccggtc cggtgacgcg catcgttccc tgcgccagca cggccttgaa ggcacggtcc
     1321 aggagtccgt acatgattgt atcctcccaa agacacgccg ccggcccgtc cgcaggcggt
     1381 cattcccccc gaagtgttaa tgaaagttaa tacaaggctt cgggaccccg caagagggag
     1441 gaatctgctg agttaacagg ggtgccgcta ggtcaggatg ccatagaaaa agggccgccc
     1501 cgaagggcgg ccctcatccg ttttggtgcg agccaagccg cggtcaggcg ttgtcctgcg
     1561 cctcggcttc ctgctcgaac agggcctcgg cggccgcgcg gatctcggcg cggtcggaga
     1621 ccgggccggt gaggtcctcg ccgcgggcct gggcgtccgc ctcgtcggcg gagcgggcga
     1681 cgttcaccgt caccttcacc tcgacctccg ggtgcggaat gaccgacacg gtgtagaggc
     1741 cgatggtctt gatcggcgca tcgagatgca cgtggccgcg ctcgaccttc acgccggtgg
     1801 cggtggccgc ctcggcgatg tcgcgcgggg tgaccgagcc gtagagctgg cccgactcgc
     1861 cggcctggcg gatcagcacg aaggtcttgc ccgcgaggcc cttggcctcg gcctcggccg
     1921 cgccgcgccg gtcggcgttg cgggcttcga gatcggcgcg atcgcgttcg aacttggcct
     1981 tgttggcgtc ggtggcgcgc agcgccttgc cgcggcggag caggaagttg cgggcatagc
     2041 cgtccttgac cttcaccact tcgcccatgg cgccaagctt ggcgacgcgt tcgagaagaa
     2101 taacgtccat tggtccggtc tccttacttc agcacatagg gcagaagacc caggaagcgg
     2161 gcgcgcttga tggccttggc cagctcgcgc tgcttcttgg cgcagacggc ggtgatgcgg
     2221 gacggaacga tcttgccgcg ctcggaaatg tagcgctgca gcagacgcgt gtccttgaag
     2281 tcgatcttcg gcgcattgtc gccggtgaag gggcagctct tcttgcggcg gaagaagggg
     2341 cggcgtgcgg gttgaccagt cgacatcagt tcgctccttc cacttcggct tcctcgcggc
     2401 ggccacggcc accgtcacgg ctttcgcggc cttcacggcc gccgaagcca ccgccgccgc
     2461 cgaagccgcg gtcgctgcgc tcttcacggt cgtcgcggcg gcgcatctgg gccgacgggc
     2521 cttcctccag cgcttcgacc ttgaccgtca ggtgacggat cacgtcttcg ctgagcgaca
     2581 tctggcgctc catctcggcc acggcggccg cgggagcgtc gatgttcatc agcgtgtaat
     2641 gcgccttgcg gttcttcttg atgcggaagg agagggactt gacgccccag tactccgtct
     2701 tggtgacctt gccgccgccg gcggcgatga ggcccttgaa ctgctcggtc agggcatcga
     2761 cctgcgtgtt cgatgcatcc tggcgcgtca ggaatacgtg ctcgtagaga gccatgtggg
     2821 atagcctttc ttcattacgt ttcagcgact tgcatccggc gcagagcctc tttcgaacct
     2881 ggttgaaggc tcgaaagacc ggtcaaacga aagagcggag acacgggaag acggggtcga
     2941 agcccctgat tgccaaggca atccttccgt tcagccccca gccgggtgcc tatcggcagg
     3001 cggcttatgg cggataagcc cggcgattgc aacccctgcg actcttgcgg ctctggcgga
     3061 tcgccgcagg tggaggcatg gtcccgcgca tgagctgccg ggggacagga cagaagcgcg
     3121 cggcgcgggc aggggtagcc ctgagcctgc tctttgcgtt cctgctgcca ttgttgctcg
     3181 gcgtgctccc ggcgccaacc gcggccgctc agctcgccca ggacatcgcc gccagctact
     3241 gcgacccgaa cggcgagcgt ccggcgcagt cgccgcatgg cgatcacaag acctgctgca
     3301 tcctctgccc ggttggcggc cttgctgcgc ttgaagcgcg cgacgtgccc atcgatccgg
     3361 cgcccgccgc gcaggcggac gtctgggtgg ccggcgtcgg cctgccgctg ctgcccggcc
     3421 gccgcatcga tctggcccag atcgttccgc gaggccctcc atcgccgtga tgacctgaat
     3481 gcgtatccgc gggccgcact gcgcgcgccc gccgtccttc atcatcactt tgcgaggtct
     3541 tcatgtcgtc gacaaccctg ccgcagcaag acgctgtggc cagcacgcgg ctctaccggg
     3601 ccatgtggcg ctggcatttc tatgccggcc tctatgtcat tccctttctc atcatgctgg
     3661 ccgtcaccgg cctcatcatc ctgtggttca ccgccattgc gcccgagtac ggcgaccggc
     3721 tgacgctgca gcccggcgcc caggcgctga gcatcaccca gcaggccgat gccgcgctgg
     3781 ccgcacaccc cggcggcacc gtcggcaaat atgtcccgcc gctcgtcccc gaaaatccgg
     3841 cgctgttcca ggtcaatctg gaacagggca accgcatgat tgcgatcgat ccctataccg
     3901 gagcgatcct gcgcgaccgt gcgcaggatg gaacctggaa cgagttcgcc accgacatcc
     3961 acggctcgct gctgctgggc ggcagcggcg gcttcggcga ccttctgatc gaagtggcgg
     4021 ccagcctcgg catcgtgctg ctgatcaccg gtctttacct gtggtggccg cgcggcaccg
     4081 gctggcgcga tgtcctggtg ccgcggcttt cggcgggcgg ccgcgccttc tggaagtcga
     4141 tccactcggt caccgggttc tggatgtcgc tggtcatcct cttcttcctg gtctcgggat
     4201 tgtcatgggc gggcgtctgg ggcgggaagt tcgtgcaggc ctggagcacc ttcccggccg
     4261 agaagtggga cagcgtgccc ctgtccgatg aagcccttgc cgggatgaac cacggcgccc
     4321 tcaaggaagt gccctgggcg ctggaacaga ccctgatgcc ggcctcgggt tcggcggcag
     4381 gcgtcaccgg cctgccggaa ggcgtgcccg tggtactcga gtcggtggtg gcgctgggcc
     4441 gtgccatcgg cttcgaaggc cgcttccagg tggccgtacc cgccgacaag acgggggtct
     4501 ggacgctgag ccaggactcg atgagctacg acagccccga tccgacagcc gaccgcacgg
     4561 tgcatgtcga ccagtacacg ggcaagattc tcgcctcggc cgcctttgcc gactattcgc
     4621 tggccggcaa ggcgatggcc gtgggcatcg ccctgcacga aggccagatg ggattgtgga
     4681 acatcgtcct caatgtcgtc ttctgccttg ctgtgatcct gatcgcggtc agcggtgtgg
     4741 tcatgtggtg gctgcgccgg ccagtgggcc agttcggcgt gccgctctat ccgaaagcct
     4801 atcgtgtgcc ggtggccatc cttggccttg cggtgctggt gtccattgcc ttcccgctgg
     4861 gcggtgccgc catcgcgctc atcgccgtgg tcgacttcct tctgccgaag cgcgtgaagg
     4921 aagccggttc cgccaaagcc tgacaacatc cacaagaaag gaatccgatc atgaaactct
     4981 tcaaggaact cgccttcgcc gcgacactct cgctggcctc gatctatgcc atcgcctcgg
     5041 gccacgcgca cgagatcaag gttggcgaca tcgtcatcgc gcatccctgg tcgcgccccg
     5101 cgccgatggc gtcggacgtc gctgcaggct tcatgaagat aaccaacacc ggcaccgcag
     5161 acgacaggct cgtcagcgcg accgccacca tcaccggcac ggtgcagctg catgacatga
     5221 agatggaggg cgacgtgatg aagatgttcg agatcccggg tggtatcgtc attccggccg
     5281 gccagaccgt tgaactcaag cccaagtcgc tccatgtcat gttcatggat ctcaagcgtg
     5341 caccggtcga gggcgaggcg ttcaagggaa cgcttacctt cgagaaggcc ggcacggtcg
     5401 agatcgacta cgaggtgacg gcgcaggccc cggaggccca ttgatgctgc ggttcatccg
     5461 tattgcaagc ctggtgacgg cggcgcttct cgccgccgtc accggttacg tctatctcac
     5521 ccgcggcgag gcggtggtga gcgttgccga catcggcggc cccttccgcc tggcctcggc
     5581 cagtggcggg gtcgtcgaca gccaggacct ggcaggaaaa ccctatgccg tcttcttcgg
     5641 cttcacccgc tgccccgaag tgtgcccgac cacgatggcg gagatgagtg ccgcactcac
     5701 cgaactcggc gacgaaggca aggatctgcg ggtcttcttt gtcaccgtcg atccggaacg
     5761 cgatacggcg gagttcctga aggactatct ctccagcttc gatccccgca tcgaagcgct
     5821 cgtacccact gcagaggagc ttgcggccat ggctcgcagc tttcgcgctt actacgccaa
     5881 gtcgccgacg tccgatggcg gatacaccat ggaccacacg gccacggtct tcctgatgaa
     5941 ccggcagggc aagctcgcct cgaccatctc ctacggcgag gaacaggcaa cgcgcatggc
     6001 caagctgcgc aagctgctcg gcgtgtaagt tccggatcgc tgtgctaggc tcgcttgccc
     6061 gatcttgttc ttgttggttt tggtcatgcg cagcaaatcg ctttcggtca tcctgttcgt
     6121 cctgtccctt gccgaggcca gggccgagga cggcgttggc cttgcaccgg ccgtggcggc
     6181 catcttcccg cgcggcgagg aggtctgcta tctcgccggt cccgaaacca caggcctgaa
     6241 atccgggcag aagctgtctg agttctatct ctaccggttc tatgaccccc tgcacgcgcg
     6301 cgaggagatc gggccgtcgc gcgaagaggc catcgcgcgc gagcggaaga tcaattcgtc
     6361 ctatgccagc ctcgatgtgg tggcgcgttt ccgggattcg cgaaacgtct actcccagtc
     6421 ggtcctgtgc accacttatg acggcggcaa gaccatcacc tgcggcgtcg aatgcgatgg
     6481 cggcagcttc gcggttgaac cgggcaaccg ggctctccag atcaccgttc cagccgacgg
     6541 cagcggcctg tcgctcaacc agagctgcgg cgatcccgac gacgaggggc gcgagcgctg
     6601 gctcaccggt gccgatgcgg gcgggtcctt cacgctcgag cgctggccga tggcccattg
     6661 catcgcggcg gccgaggatg cccatcccg
//
LOCUS       DBNGVR010000179         3556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2814764, whole genome shotgun sequence.
ACCESSION   DBNGVR010000179 DBNGVR010000000
VERSION     DBNGVR010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2814764"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..861
                     /locus_tag="AC16A0_03870"
     CDS             121..861
                     /locus_tag="AC16A0_03870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3104499.1"
                     /translation="MKVAVVTGAGSGIGKAVAQAFGRDGHAVVLAGRRADALETVARS
                     IGSNALAVPTDVSDPASVAKLFAAAKAKFGRVDVVFNNAGTGAPGTILLEDLTIEQWQ
                     NVVNVNLSGVFYCMQEAFRIMKAQTPRGGRIINNGSISAHAPRPNSAPYTSTKHAVTG
                     LTKSGSLDGRKYDIAVCQIDIGNAGTDMTEKMKSGMPQANGTVAPEPTMDVENVARTV
                     LYMASLPLEANAQFVTVMATKMPFIGRG"
     gene            complement(871..1314)
                     /locus_tag="AC16A0_03875"
     CDS             complement(871..1314)
                     /locus_tag="AC16A0_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006014199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HIR3104500.1"
                     /translation="MIRYDLICDKGHEFDGWFRDSATYDKQARRGFVACSVCGSSKVE
                     KQLMAPGIPAKSNRKDEVPQRMVAGPVDPRVAMMMKMMREMRQHVEENADYVGDKFAE
                     EARKIHFAETEARGIYGEATADEATALIEEGIEVFPLPRLPEDGN"
     gene            complement(1338..2168)
                     /locus_tag="AC16A0_03880"
     CDS             complement(1338..2168)
                     /locus_tag="AC16A0_03880"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3104501.1"
                     /translation="MILRAGLVQLRSGRDMARNLADAAGLIRQAAGQGAALVATPEMT
                     NVLEPDRPRLRGLAKVQAEDESVAGFSALAVELRIWLNIGSLALMGDDGTLINRSLLF
                     DPAGTLRAYYDKIHLFDVDLPTGESLRESHAYSGGSRAVVLDAAGYRLGLTICYDVRF
                     PGLYRHLAQAGAEVITVPSAFTVPTGEAHWHVLLRARAIETGSFVLAAAQAGRHESGR
                     ETYGHSLAVAPWGEVLAEAGQDPCAVVVDLDLAQVTAARRRIPALAHDRPFLLENLKR
                     "
     gene            complement(2165..2428)
                     /gene="grxC"
                     /locus_tag="AC16A0_03885"
     CDS             complement(2165..2428)
                     /gene="grxC"
                     /locus_tag="AC16A0_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HIR3104502.1"
                     /translation="MNKAKITIYTTPYCPYCHAAKALLNRKGAAFEEIDVQDPALRQQ
                     MMLRANGRRTVPQIFIGEIHVGGSDDIHELDRRGGLDSLLVSA"
     gene            complement(2433..3188)
                     /locus_tag="AC16A0_03890"
     CDS             complement(2433..3188)
                     /locus_tag="AC16A0_03890"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR3104503.1"
                     /translation="MDVAELRLKAWRGLVDFLTPSQCLICHQPAGEAQGLCAACWAGL
                     THLDEPACNILGTPFTYEQGPTAVSVAALANPPAWDRARAAVAFDDRSRLLVHALKYR
                     DTPQAGHLMARMMCRAGRRLVAEADLILPVPLFRWRLWRRRFNQSAVLAQEIARISGT
                     PWTPTLLLRQRATRSQVGLNVSERRRNVRGAFMLPADGVGQVKDKAILLIDDVRTTGA
                     TLEACTDVLRAAGARQVDVLTFALVLEPARLHN"
     gene            3280..>3556
                     /locus_tag="AC16A0_03895"
     CDS             3280..>3556
                     /locus_tag="AC16A0_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3104504.1"
                     /translation="MTFRRTDLDRLAAIMTEAAETQIMPRFRSLGDGGIREKTGALDL
                     VTDADEKAEWQIRDACAKAFPTARFIGEESVERDRSLLDGIGDAELAI"
BASE COUNT          652 a         1177 c         1130 g          597 t
ORIGIN      
        1 aggctgtgcc ggcttcggca ttggctccaa catttacaaa cccggcatga ctgccgatga
       61 tgtggcgaca tccgcggcag gcttcgttgc ggcatggaat tcattgaaga ggaacggacg
      121 atgaaggtag cagtggtgac aggcgccggc agtggcatcg gcaaggccgt ggcgcaggcc
      181 ttcgggcgcg acggccatgc cgtagtcctg gccggacggc gggccgacgc gctggagacc
      241 gtggccaggt ccatcgggtc caacgcactg gccgtgccga ccgacgtgtc cgatcccgct
      301 tcggtggcaa agctcttcgc cgccgccaaa gcgaagttcg gccgcgtcga cgtcgtgttc
      361 aacaatgccg gcaccggcgc acccggcacg atcctgctcg aggacctcac catcgagcag
      421 tggcagaacg tggtgaacgt caatctctcc ggcgtcttct actgcatgca ggaggccttc
      481 cggatcatga aggcgcagac gccgcgcggc ggccgcatca tcaacaatgg atcgatttcc
      541 gcccatgcgc cgcgccccaa ttcggcgccc tacacctcca ccaagcacgc cgtgacgggc
      601 ctcacgaaat cgggctcgct cgatggacgc aaatacgaca tcgccgtctg ccagatcgac
      661 atcggcaatg ccggaaccga catgaccgag aaaatgaagt cgggaatgcc gcaggccaat
      721 ggcacggtgg cgccggagcc gaccatggat gtcgagaacg tggcccgcac cgtgctctac
      781 atggcctcgc tgccgctgga ggccaatgcg cagttcgtga cggtaatggc caccaagatg
      841 ccattcatcg ggcggggctg aacggcaccg tcagtttccg tcttcgggca gtcgcggcag
      901 gggaaagacc tcgattccct cttcgatcag cgcagtcgcc tcatccgcag tggcctcgcc
      961 atagattccg cgcgcttcgg tttcggcgaa atggatcttg cgggcttcct cggcgaactt
     1021 gtcgccgaca taatccgcat tctcttccac atgctggcgc atctcgcgca tcatcttcat
     1081 catcatggcc acgcgcggat cgactggacc tgcaaccatt ctttgcggca cctcgtcctt
     1141 gcgattggac tttgccggga tgccgggcgc catgagctgc ttctcgacct tcgacgagcc
     1201 gcagacggaa caggccacga atccgcgtct tgcctgcttg tcataggtag cgctgtcgcg
     1261 gaaccagcca tcgaattcgt ggcccttgtc gcagatgagg tcatagcgga tcatgctgtc
     1321 tagctagcaa tgcccagtca gcgtttcaag ttttcgagca aaaagggccg gtcatgggca
     1381 agtgccggta tcctgcgcct tgccgcggtc acctgcgcga ggtcgagatc gacgacaact
     1441 gcgcaggggt cctgtccggc ttcggccagt acctcgcccc agggtgcaac cgccagcgaa
     1501 tgtccatagg tctcacgccc cgattcatga cggccagcct gcgcggccgc aagaacgaag
     1561 cttcctgtct cgatcgcccg ggcccgcagc agcacatgcc agtgggcttc gccagttggg
     1621 acagtgaagg ccgagggcac ggtaatcacc tctgcaccgg cctgcgccag gtgtcggtaa
     1681 agacccggaa agcgcacatc gtagcagatg gtcagtccca gccggtatcc cgcggcatcg
     1741 agaaccaccg cgcggctgcc gcctgaatag gcatgcgact cgcgtagcga ctcgccggtc
     1801 ggcagatcga catcgaagag atgtatcttg tcgtagtagg ctcgcagcgt tccggcgggg
     1861 tcgaagagca gggatcggtt gatcagcgtg ccgtcatcgc ccatcagcgc cagcgagccg
     1921 atgttgagcc atatccgaag ctctacggcg agcgcggaaa agcctgcaac gctttcatct
     1981 tccgcctgca ccttcgccag gccacgcagg cgcggcctgt caggctcgag aacattggtc
     2041 atttccggcg tcgcgacgag tgccgcacct tgccccgccg cttgccggat caggccggcc
     2101 gcatcggcga ggttccgggc catgtcgcgg cccgaccgca gctggaccag gcccgcgcga
     2161 aggatcacgc gctgacgagc aggctgtcga gcccgccgcg gcggtcaagc tcatggatat
     2221 cgtcgctgcc gcccacatgg atctccccga tgaagatctg cggtacggtc cgccgcccat
     2281 tggcccgcag catcatctgc tggcgcagcg caggatcctg cacgtcgatc tcttcgaagg
     2341 ctgcaccctt gcggttcagg agcgccttcg cggcgtggca gtaagggcaa tagggcgtgg
     2401 tgtagatggt gatcttcgct ttgttcatga tgctaattat ggagacgcgc cggttccaga
     2461 acaagagcga aggtcaatac atccacctgc cgcgcgcccg cggcgcgcag cacatcagtg
     2521 caggcttcca gcgtggcgcc ggtggtgcgg acatcgtcga tgagcaggat ggccttgtcc
     2581 ttcacctgcc ccacgccatc cgccggcagc atgaaggctc cccgcacatt gcggcggcgc
     2641 tcgctgacat tgagccccac ctggctgcgg gtggcccgtt gccgcagcag gagcgtgggg
     2701 gtccatggcg tgccggagat ccgggcaatc tcctgcgcca gcacggcaga ctggttgaag
     2761 cgccgccgcc acaggcgcca gcggaacagg ggtaccggca ggatcaggtc ggcttcagcc
     2821 accagccggc ggccggcccg gcacatcatg cgggccatga gatgtcccgc ttgcggcgta
     2881 tcgcggtact tgagcgcgtg gaccagcagc cgcgacctgt cgtcgaaggc gacggccgcc
     2941 cgcgcccggt cccaggccgg cgggttggcc agcgccgcca cgctcacggc cgtcggcccc
     3001 tgctcatagg tgaaaggtgt gccgaggatg ttgcatgcag gctcatcgag atgggtgagc
     3061 ccggcccagc aggccgcaca gaggccctgc gcctcgccgg ccggttgatg gcagatcaga
     3121 cattggctgg gggtcagaaa gtccaccagc ccgcgccagg ccttcagccg cagctcagcc
     3181 acatccatcc gcatgccgcc atgctagcag agcgggcgag aacattggat tcctaaactg
     3241 cgatcgtcta accagtggcg cgaacggagc accccagaca tgacctttcg ccggaccgac
     3301 ctcgaccgcc tcgctgccat catgacggag gcggccgaaa cacagatcat gccgcggttc
     3361 cgaagcctcg gcgatggcgg catccgcgag aagaccggcg cgctggacct ggtgaccgat
     3421 gccgacgaga aggccgaatg gcagatacgg gacgcctgcg ccaaggcctt ccccaccgcc
     3481 cgcttcatcg gcgaggaatc ggtggagcgc gaccgcagcc tgctcgacgg catcggcgat
     3541 gcggaactcg ccatca
//
LOCUS       DBNGVR010000180         4597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2620094, whole genome shotgun sequence.
ACCESSION   DBNGVR010000180 DBNGVR010000000
VERSION     DBNGVR010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2620094"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC16A0_03900"
     CDS             complement(<1..529)
                     /locus_tag="AC16A0_03900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971144.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3104505.1"
                     /translation="MPQYSPDQTRSMANAIRALSMDAVQKANSGHPGLPMGAADIATV
                     LFTQFLKFDAADPHWPDRDRFILSAGHGSMLMYSLLYLLGYEDMTLDEIKNFRQLGSK
                     TAGHPEFGHATGIETTTGPLGQGIANAVGFAIAERHLNAGFGDDLVNHKTYVLAGDGC
                     LMEGISQEAITLAGHL"
     gene            705..989
                     /locus_tag="AC16A0_03905"
     CDS             705..989
                     /locus_tag="AC16A0_03905"
                     /inference="COORDINATES: protein motif:HMM:NF025122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HIR3104506.1"
                     /translation="MAGTEKLDEAFDRFERALRQFEGAVGRTQQTEKRAETLAGEAEA
                     LRRDRARLAHELDLVRSKAGELVDTSKQAAGKIDAAMSRIRAVLHSNSPD"
     gene            993..1349
                     /locus_tag="AC16A0_03910"
     CDS             993..1349
                     /locus_tag="AC16A0_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR3104507.1"
                     /translation="MAHVIVQVNGRPYTMQCPDGEEAHLRDLAKLLDAEVARVKASVG
                     SVGDIRLLVMSGLMVADRLSEATARIEALEEQVRGLRESRNAALAEARELEAQFIARL
                     DSASKRLLGLAQDIDG"
     gene            1400..1561
                     /gene="ssrS"
                     /locus_tag="AC16A0_03915"
     ncRNA           1400..1561
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16A0_03915"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1583..2158
                     /locus_tag="AC16A0_03920"
     CDS             1583..2158
                     /locus_tag="AC16A0_03920"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR3104508.1"
                     /translation="MSIAEIKKSARGAASKRRAEAHAALKDEAGLALAARGLPDGLAT
                     RRGIVSGFIPYKSEITTLPLMAALLRLGWQTALPVVIAPDAPLVFRPWSPGDRLEPGV
                     WDIPVPLAAAPEVLPDLLLVPGLSFDRKGFRLGYGGGFYDRTLAKLRAVKPVTAIGVA
                     YHAQIVDEVVRDAFDAPLDYVMTEQETIACG"
     gene            2149..2970
                     /locus_tag="AC16A0_03925"
     CDS             2149..2970
                     /locus_tag="AC16A0_03925"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR3104509.1"
                     /translation="MRLIFLGDVVGRSGRDALARELPLLRERYRPDLVVVNGENAAHG
                     FGITEDIFLSLVAAGADVVTLGNHAFDQREALIFIERYPNLLRPLNWPAGCPGRGTAL
                     VDTASGRRVLVMNAMGRVMIEPVLDDPFPAVSRELEACPLGVACDAVLIDFHAETTSE
                     KMSMGHFCDGRASLVVGTHTHVPTADHQILPGGTGFMSDAGMCGDYDSVIGMEKAEPL
                     NRFQRKLPVERMRPAEGPATVCGVAVETDDATGLARAIAPIRVGGRLSEAAVSFW"
     gene            3250..4296
                     /locus_tag="AC16A0_03930"
     CDS             3250..4296
                     /locus_tag="AC16A0_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104510.1"
                     /translation="MKKIATLLGAVLLAGTAFTASVRAADELTITSWGGAYQESQRKA
                     FFEPFIAAGNKITEAEYNGEIAKIRAMVEAGAVTWDVVDVDTQTALAGCAEGTFETID
                     WAKLGLDRSKFIGGDVTDCAVPNILYSTVAAYDTTKTEAPASLFVALFDTTKYPGKRA
                     LQKSPFVNMEWALMADGVAPADVYKVLGTDEGVERALKKLDTIKKDVVWWESGAQGPQ
                     LLADGQVVFATSWNGRFYNAIKTDKKPFQIIWDYQAPDWDWWAIPKGTPKIEEAYRFI
                     TFASDPQRMANQTKFIAYGPANKDAAPLVDPAILPDLPSAPDNLKTVFYPDPQFWGDK
                     GDELRERFNAWLAQ"
     gene            4373..>4597
                     /locus_tag="AC16A0_03935"
     CDS             4373..>4597
                     /locus_tag="AC16A0_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104511.1"
                     /translation="MAMAEALPNDGVMRAADGTPLKVALARAERRERIKALGLVTPLF
                     AFIVLSFLIPILVMLYNSVYDPDVAENLPAT"
BASE COUNT          790 a         1468 c         1507 g          832 t
ORIGIN      
        1 tgaggtggcc ggccaaggtg atggcctcct ggctgatgcc ctccatcagg cagccgtcgc
       61 ccgccagcac ataggtcttg tggttgacca ggtcgtcgcc gaatccggca ttgagatggc
      121 gctcggcgat ggcgaagccc acggcattgg caatgccctg gcccaacggg ccggtggtgg
      181 tctcgatgcc ggtggcatgg ccgaattccg gatggccggc ggtcttcgag cccagctggc
      241 ggaagttctt gatctcgtcc agcgtcatgt cctcgtagcc caggagatag agcagcgaat
      301 acatcagcat ggagccatgg ccggcggaga ggatgaagcg gtcccggtcg ggccagtgcg
      361 gatcggcggc atcgaatttg aggaactggg taaacagcac ggtggcgatg tcggcagccc
      421 ccatgggcag gcccggatgg cccgaattgg ctttctggac cgcatccatg ctgagggccc
      481 ggatggcatt ggccatggag cgtgtctgat ccggagaata ctggggcatc tgggggaatt
      541 cctcgcaagc ggttcgagcc gggctcgtga taacacccgg cccccgagag tcaattgtgg
      601 atcgtaagtc gcttttcgct ggcggcgttg cacttcggcc acgttccccc tatgattcgt
      661 gaaatcttca ggagacagat tctcttgtct tcacagaggc tttgatggca ggcactgaga
      721 aattggatga agctttcgac cgcttcgaac gggccttgcg gcagttcgag ggcgcggtcg
      781 gccgtacaca gcagaccgag aagcgcgccg agacgctggc gggcgaggcc gaggccctgc
      841 gccgcgatcg cgcgcggctt gcgcatgaac tcgacctggt gcgcagcaag gccggcgagc
      901 tcgtcgatac cagcaagcag gccgccggca agatcgacgc cgccatgtcg cgcatccgcg
      961 ccgtgcttca ttccaacagt ccggattgag ccatggccca tgtgatcgtt caggtgaatg
     1021 gccggcccta caccatgcag tgcccggatg gcgaagaggc gcatctccgc gacctcgcca
     1081 agctgctcga cgccgaagtg gcgcgggtca aggccagtgt cgggagcgtg ggcgacattc
     1141 gtctgctcgt catgtcggga ctgatggtgg ccgaccggct ctcggaggcg accgcgcgca
     1201 tcgaggctct cgaggaacag gtgcgcggac tgcgggaatc gcgcaatgcg gcactcgctg
     1261 aggcgcgcga gcttgaggcc cagttcatcg cccggctcga ctccgcctcc aagcgcctgc
     1321 tggggcttgc ccaggacatt gacggctgac gctccggccc aattggacat ttctcccgcg
     1381 acttcctaca ttggccggtg gcgggctgcc cggtgcgtgc ggagatttta ttccctgggg
     1441 cctatgcgat tctgaatggg agctgtccct gggcttggcc gtggcctcgc ctaaacggcg
     1501 cccacctacg taagtaggga cccgggattg cacttctcac acggcccatg gtggctcgcc
     1561 gcttctgcct cgaaagactg gaatgagcat cgccgagatc aagaagtccg cccgcggggc
     1621 cgcatcgaag cggcgtgccg aagcccatgc ggcgctgaag gacgaagcgg gcctcgcgct
     1681 ggccgcgcgc ggcctgccgg atggcctggc cacccggcgc ggcatcgtct cgggcttcat
     1741 tccctacaag agcgagatca ccaccctgcc gctgatggcc gcgctgctcc gcctcggatg
     1801 gcagaccgcg ctgcccgtcg tcatcgcgcc cgatgcgccg ctggtcttcc gcccctggtc
     1861 gccgggcgac cggctcgagc ccggcgtctg ggatattccg gtgccgcttg cggccgcgcc
     1921 cgaagtcctg ccggaccttc tgctggtgcc cggcctcagt tttgaccgca agggcttccg
     1981 cctcggctat ggcggcggct tctacgaccg cacgctggcg aagctgcgcg ccgtgaagcc
     2041 ggtcaccgcc atcggtgttg cctatcatgc ccagatcgtg gatgaggtgg tgcgcgatgc
     2101 cttcgatgca ccgctggact atgtgatgac cgaacaagag accattgcgt gcggctgatc
     2161 tttctgggtg acgtggtcgg ccgcagcggc cgcgatgcgt tggcgcgtga attgcccctg
     2221 ctgcgcgaac gctatcgtcc cgatctcgtg gtggtgaatg gcgagaatgc cgcgcatggc
     2281 ttcggcatca ccgaggacat tttcctcagc cttgtggcgg cgggcgccga tgtcgtgacc
     2341 cttggcaatc acgccttcga ccagcgcgag gcgctgatct tcatcgagcg ctatcccaat
     2401 ctgctgcgcc ccttgaactg gcctgcgggt tgtccgggcc gtggcacggc gctggtggat
     2461 accgccagcg gcaggcgggt gctggtgatg aatgccatgg gccgggtcat gatcgaaccg
     2521 gtgctcgacg atccctttcc cgccgtttcg cgcgaactcg aggcctgccc gctgggcgtt
     2581 gcctgcgatg cggtgctgat cgactttcat gccgagacca cctccgagaa gatgagcatg
     2641 gggcatttct gcgatggccg cgccagcctg gtcgtcggca cccacacgca tgtgcccacc
     2701 gccgatcacc agatcctgcc gggcggcacg gggttcatgt ccgatgccgg catgtgcggc
     2761 gactacgact cggtgatcgg catggagaag gcggagccgc tgaaccgctt ccagcgcaag
     2821 ctgccggtgg aacgcatgcg gccggccgaa gggccggcga cggtgtgcgg cgtggcggtg
     2881 gagaccgatg acgccaccgg cctcgcgcgc gccattgcac cgatccgcgt cggcgggcgg
     2941 ctgtcggagg ccgcggtttc cttttggtaa gtgccgtcca tgacctaatg tctggtgacc
     3001 cagcaaaaaa actctagtga cgcgctgcaa aaagccgtta gtatctttca ccagcgcttg
     3061 gaaaagcagt gccgggaagg gtcgcgcaca gggttgcgac aggtccttca acaggccgag
     3121 aaagcgttga atatggggag gatcgccgga tcatgcaggt agcgccgaag cgggcgttcg
     3181 ttgcgatatc catcctcctc gaaacgtttt gacgccgggt gtccggccta aaaggaggaa
     3241 gagaccagta tgaagaaaat cgcaacactg ctcggtgcgg tcctgctcgc cggaacggcg
     3301 ttcacggcct ccgtgcgcgc cgccgacgag ctgaccatca cgtcctgggg cggcgcctat
     3361 caggaaagcc agcgcaaggc cttcttcgag cccttcatcg cggcgggcaa caagatcacc
     3421 gaagctgaat acaacggcga gatcgccaag atccgcgcca tggtggaagc cggcgccgtg
     3481 acgtgggacg tggtcgacgt cgatacccag acggcccttg caggctgcgc cgaaggcacc
     3541 ttcgagacca tcgactgggc caagctcggc cttgaccgct cgaagttcat cggtggcgac
     3601 gtgaccgact gcgccgttcc gaacatcctc tattcgacgg tcgctgccta cgacaccacc
     3661 aagacggaag ccccggccag cctgttcgta gcgctcttcg acacgaccaa gtatcccggc
     3721 aagcgcgccc tgcagaagag ccccttcgtc aatatggagt gggcgctgat ggccgatggc
     3781 gttgctccgg ccgatgtcta caaggtgctg ggcacggatg agggcgttga acgcgcgctc
     3841 aagaagctcg acaccatcaa gaaggacgtc gtctggtggg aatcgggcgc ccagggtccg
     3901 cagcttctcg ctgacgggca ggtggtgttc gccacctcct ggaacggccg cttctacaac
     3961 gccatcaaga cggacaagaa gcccttccag atcatctggg actaccaggc tccggattgg
     4021 gactggtggg cgatcccgaa gggcacgccg aagatcgagg aagcctaccg cttcatcacc
     4081 ttcgcgtccg acccgcagcg catggccaat cagaccaagt tcatcgccta cggcccggcc
     4141 aacaaggacg ccgctccgct ggtcgacccg gcgatcctgc cggacctgcc gtctgctccc
     4201 gacaacctca agaccgtgtt ctatccggac ccgcagttct ggggcgacaa gggcgacgag
     4261 ctgcgcgagc gcttcaacgc ctggctggcc cagtaatcac gtcttcggcg cgggaagagg
     4321 gccctctgcc ttcttcccgt tttcattcca ccggcatcga ggggcagttt ccatggcgat
     4381 ggctgaggct ctacccaatg acggcgtgat gcgcgccgca gacggcacgc cgttgaaagt
     4441 tgcgctggcc cgtgcggaac gccgcgagag gatcaaggcc ctcgggctcg tcacgccgct
     4501 gttcgccttc atcgttctgt ccttcctcat tcccatcctc gtgatgctct acaattcggt
     4561 ctacgacccg gacgtggccg agaacctgcc cgcgacc
//
LOCUS       DBNGVR010000181         5972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4781551, whole genome shotgun sequence.
ACCESSION   DBNGVR010000181 DBNGVR010000000
VERSION     DBNGVR010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4781551"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16A0_03940"
     CDS             <1..307
                     /locus_tag="AC16A0_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104512.1"
                     /translation="GSGFILAAVLTLVTAGTLGGMGSHWIGGDQTDATGLPLLGWSTT
                     GGDLRVSHFVSLHLMQAIPLAALTGRSLAVLAVAVAGIAAAAATYAQALMGYPLLAF"
     gene            386..1132
                     /locus_tag="AC16A0_03945"
     CDS             386..1132
                     /locus_tag="AC16A0_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR3104513.1"
                     /translation="MKPTLILVGADKGGVGKTTISRALLDFFARKNVLTRAFDTENPR
                     GSLKRFYPAITEVIDLENVAHQMKVLDTMETANVPVTLVDLKAGGLSYALDIFERIGV
                     LEAARNNQFTLGLFHIVGSSIASLDEIGEISKYLNGIEYVVTRNFINETNFFEWDQTT
                     HRKYFANLPKAQEIDIPKLNEMAYEQIDLSGVTFKDFIENRAADGQPGKFSFVLRGYA
                     RKWCSEIDEEVARVKMVQDLLTGGRGTPAA"
     gene            1149..1844
                     /locus_tag="AC16A0_03950"
     CDS             1149..1844
                     /locus_tag="AC16A0_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104514.1"
                     /translation="MASRFAVHIVCSDQPRNGKSLLARVLSDFLLLDGRDPFLIDISH
                     PEGTLRNYFPGRTVLVDFSNIRGQMKAFDTIMQAPGRDYVIDVSSPYLDRFCEAAKNL
                     NLSEAFREVDLAMAVFFVVDAAPDSLKAAAWAEKMLKADIFVPVRNTAVGSALPARFD
                     GMTLNMPRLDPELHAIIENRRFSLRTFLQGDEADVPVRLRPRLKDFLQSVMTELRAVA
                     PALSLVRLRSGAA"
     gene            1967..3121
                     /locus_tag="AC16A0_03955"
     CDS             1967..3121
                     /locus_tag="AC16A0_03955"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3104515.1"
                     /translation="MPRSNPHSLQVDCLRSLAEAEAFAPAWRELEDGHATSLDWFQTS
                     GWCLNWLRHLGHEGIAPRILVLRDGGEAIAVWPLMIETERFGLRILRSLGDPHTQYSG
                     ILCRNGTLESTHVELLRSALLSLVDADAAVIKLVPADSPLARVLPAGSCIEALGTSAS
                     VLSLAEFRTAADYVARLSKARRRSRSRRSKGLAERGTVSFRALQPEHPDYARILTRAM
                     AYKKSWLARTGRVSLGLSMPGHADFLAGIGKGPVLFELAVNGETIAAELGFLRNRHFY
                     SYLGSFDWAWRDLSPGKAQMDLALEWLIAQGAEAYDLLAHRTSYKDSWSDTEIPLHGH
                     VVEITTRGAIYARCWINTARPALKRAMASIPEEYRAGLQIVMKQDIPFLA"
     gene            3114..4418
                     /locus_tag="AC16A0_03960"
     CDS             3114..4418
                     /locus_tag="AC16A0_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3104516.1"
                     /translation="MRNAAFTFAIRVAGAGAAIGLQILLARLMGADSYGLYVIAWTWM
                     LALGSVVSLGLADAAMRFVPRYVARGRAPQAARFFRTGVGSVTLAGLLLATAGAGILQ
                     VLPLDRPLLALVTVVAVGLPIVAFEYFLEGMARSLGWFRLTGIAIYIVRPMLIAVACL
                     ALHGAGVGLTAPLVGTVTILAVAATGLTTYFTLAMHLRPATGPRRPAGRLRRLWLGAA
                     LPMLVVSSLDDLFPALDVMLVGALLGPAEAAIYFAAGRLLALAHFAQYAVHFVNGHAM
                     SLALAGNAEQSVARCLQRATAVSALATLVALGATLAAGPFLLGLFGQNFVHGYAAMCL
                     LAVGLLGRSLAVPAQEYLLLSGRNRVLTRIGAITLAVLAVLCAVLAPAFGLTGAATAA
                     AIAQLLRSLLLIRATRRMGGLLVLPPLRLGRQRFIAAPGGSQ"
     gene            4454..4846
                     /locus_tag="AC16A0_03965"
     CDS             4454..4846
                     /locus_tag="AC16A0_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015007508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3104517.1"
                     /translation="MDRFIGGCLCGDVRFVATGRPYRVGLCHCMDCRKHHGAVFHASA
                     IFPESAVSVEGGTRDYCGRHFCPRCGSSVFARTGDEVEVNLGSLDTPDQLRPTYESWT
                     SRRESWLPPFPLKRRYERDRDATGRFED"
     gene            complement(4876..5865)
                     /locus_tag="AC16A0_03970"
     CDS             complement(4876..5865)
                     /locus_tag="AC16A0_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104518.1"
                     /translation="MGVRKRSGKGEIVLRLDESIEVEARASLPPLSAHKPCNPSLIVR
                     DDTILCVYKGINFDLETEGYRFTYGGYNVPFCDSQNYVAELDMDLKVRGAHFLEDRHI
                     RARPFALHGLQDLRLFAWRDGLWCMGAAATHETQSGAVRRRLVERVVLCRLAGKTLKV
                     AAVLPSRQLREKNWMPWVRGDDLFAIYAQDPYEVLHLEKGKSQQSLHPASHQALEGQF
                     GGTCVIPWQDGFIGIIHRQHRDAVRGAPASGRLRTYTHSLVVYRSNFDVAAVSVPFSF
                     EGQKVEFCCGLAVSGRSIFLSYGIWDREAVVLKAPLASVRRALNLQHVLGRDG"
BASE COUNT          947 a         2062 c         1897 g         1066 t
ORIGIN      
        1 cgggtcgggc ttcattctcg cagcagtgct caccctcgtc acggcgggaa cgcttggcgg
       61 gatgggatcg cactggatcg gcggcgacca gacggatgcg accggcctgc cgttgctcgg
      121 ctggtccacc accggcggcg acctccgcgt gtcgcacttt gtctcgctcc acctgatgca
      181 ggccattcca cttgctgccc tgaccggccg cagcctcgcc gtcctggccg tcgccgtcgc
      241 cggaatcgcc gctgccgccg caacttacgc gcaagcgctg atgggctatc cactgcttgc
      301 gttctaggtg gttccgggcg gtatactcga ctctggagcc gtgttacacc gccagtgatt
      361 ccgcggtcag cttagaggtc agatcatgaa gcccactctc atcctggttg gcgccgacaa
      421 gggcggcgtc ggcaagacca caatctcgcg cgccctgctc gacttcttcg cgcgcaagaa
      481 cgtgctgacc cgggccttcg atacggaaaa cccgcgcggc tcgctgaagc gcttctaccc
      541 ggccatcacc gaagtcatcg atcttgaaaa cgtggcgcac cagatgaagg tgctggacac
      601 gatggaaacc gccaatgtgc cggtgaccct cgtcgacctc aaggcggggg gactcagcta
      661 tgccctcgac atcttcgaac gcatcggcgt gctcgaggct gcgcgcaaca accagttcac
      721 gctgggactc ttccacatcg tcggctcctc catcgcctcg ctcgacgaga tcggcgagat
      781 ttcgaagtac ctcaacggca tcgaatatgt cgtgacgcgc aacttcatca acgagaccaa
      841 cttcttcgag tgggaccaga caacccaccg caagtacttc gccaacctgc ccaaggcgca
      901 ggagatcgac atccccaagc tcaacgagat ggcctacgag cagatcgacc tgtcgggcgt
      961 caccttcaag gacttcatcg agaaccgcgc ggccgacggc cagccgggca agttctcctt
     1021 cgtgctgcgc ggctatgccc gcaagtggtg ctcggagatc gacgaggaag tcgcccgcgt
     1081 gaagatggtg caggatctcc tcaccggcgg acgcggcacg ccggccgcct gatcccgcgg
     1141 gtgccgccat ggcctcgcgc tttgccgtcc acatcgtctg ctcggaccag ccgcgcaacg
     1201 gcaagagcct gctggcgcgg gtcctgtcgg atttcctgct gctcgacggg cgcgacccct
     1261 tcctgatcga catcagccat ccggagggca cgctgcggaa ctactttccc ggccgaaccg
     1321 tgctggtcga cttcagcaat atccggggcc agatgaaggc cttcgacacc atcatgcagg
     1381 cgcccggccg cgactatgtg atcgatgtgt cgtcgcccta cctggaccgg ttctgcgagg
     1441 cggcgaagaa cctcaacctg tcggaggcgt tccgcgaggt ggatctcgcc atggccgttt
     1501 tcttcgtggt cgatgcggct cccgattcgc tgaaggcggc ggcctgggcc gagaagatgc
     1561 tgaaagccga catcttcgtg ccggtgcgca acacggccgt gggcagcgcc ctgcccgccc
     1621 gtttcgacgg catgactctg aacatgccgc ggctcgaccc ggaactgcac gccatcatcg
     1681 agaaccgccg cttctcgctg cgcaccttcc tgcagggcga tgaagccgac gtgccggtgc
     1741 ggctgcggcc ccggctcaag gacttcctgc agtcggtgat gaccgaactc cgcgccgtgg
     1801 ccccggccct cagccttgtc cgcctgcgca gcggcgcggc ctgatcggcc gttaaccctg
     1861 aaagcccatc cacgccacgg gacaacacct ggcctgattg accaatctgg catggttctg
     1921 gctagcggtt agcaatcgtt tgctcatccg ggactcgcca agccgcatgc cacgttccaa
     1981 tccccactca ctgcaggttg actgcctccg gtcgctcgcc gaagccgagg cctttgcgcc
     2041 ggcctggcgg gagctcgaag atgggcatgc cacatcgctc gactggtttc agaccagcgg
     2101 ctggtgcctg aactggctgc ggcatctcgg ccatgagggg atcgcgccgc gcatcctggt
     2161 gctgcgcgac ggcggcgagg ccattgcggt ctggccgctg atgatcgaga ccgagcgctt
     2221 cggcctgcgg atcctgcgca gcctcggcga cccccatacc cagtattcgg ggatcctgtg
     2281 ccggaatggc acactcgaat cgactcacgt ggagctgctg cggtcagcac tcctgtcgct
     2341 ggtggatgcg gatgctgccg tcatcaagtt ggtgcccgcc gattcgcccc tggcccgggt
     2401 tctacccgcc ggcagctgca tcgaggctct gggcacaagc gcatccgtgc tgtcgcttgc
     2461 ggagttccgc accgcggcag actatgtcgc gcgcctgagc aaggcccggc ggcgcagccg
     2521 cagccggcgc agcaagggcc tggcggaacg cggcacggtg agctttcgcg ccctccagcc
     2581 cgagcacccc gactatgccc ggatcctgac ccgggccatg gcctacaaga agtcatggct
     2641 tgcccgcacg ggccgggtga gcctcggcct gtccatgccg ggtcacgccg actttctcgc
     2701 cggaattggc aagggtcccg tccttttcga gctcgccgtg aacggcgaaa ccatcgccgc
     2761 cgagctgggc ttcctgcgca atcgccactt ctactcctac ctcggcagct tcgactgggc
     2821 ctggcgcgat ctctcgcccg gcaaggcgca gatggacctc gcgctcgaat ggctcatcgc
     2881 ccagggcgcc gaggcctatg acctgctggc gcatcgcacc agctacaagg attcatggtc
     2941 cgacacggag attccgctgc acggccatgt ggtggagatc accacgcggg gggccatcta
     3001 tgcgcgctgc tggatcaaca cggcgcgacc ggcgctgaaa cgcgccatgg catccattcc
     3061 ggaggagtat cgcgccgggc tgcagatcgt gatgaaacag gatatcccct tccttgcgta
     3121 acgcggcctt cacctttgcc atccgcgtcg cgggcgcggg cgccgccatc ggccttcaga
     3181 tcctgctcgc gcggctgatg ggcgccgact cctatggact ctacgtgatc gcctggacct
     3241 ggatgctggc gctgggcagc gtcgtatcgc tgggcctggc cgatgccgcc atgcgcttcg
     3301 tgccgcgcta tgtggcgcgg ggccgggcgc cgcaggccgc caggttcttc cggaccggcg
     3361 tcggcagcgt gaccctcgca ggtctccttc tggccactgc cggcgccggc attctgcagg
     3421 tccttccgct cgaccggccg ctgctggcgc tggtgacagt ggttgccgtg gggctgccca
     3481 ttgtcgcctt cgaatacttc ctcgaaggca tggcgcgcag cctcggctgg ttccgcctga
     3541 ccggcatcgc catctacatc gtgcggccca tgctgatcgc ggtcgcctgc ctcgcgctgc
     3601 atggcgcggg tgtcggattg accgcaccgc tggtcggcac ggtgacgatc cttgcggtgg
     3661 cggcaacagg actgaccacc tatttcaccc tggcgatgca cctgcggccc gcgacagggc
     3721 cgcgccgccc ggcgggccgg ttgcggcggc tctggcttgg cgcggcgctt ccgatgctgg
     3781 tggtctcgag cctcgatgac ctctttccgg cgctcgacgt gatgctggtc ggcgcattgc
     3841 tggggccggc agaagccgca atctatttcg ccgccggccg cctgttggcg ctggcgcatt
     3901 tcgcgcagta tgcggttcac ttcgtcaacg ggcatgccat gagccttgcg ctcgcaggca
     3961 atgcggaaca gagcgtcgcg cgctgcctgc agcgggcaac agcggtttcc gcccttgcca
     4021 ccctggtcgc actgggcgcc acgctcgccg ccgggccgtt cctgctcggc ctcttcggac
     4081 agaacttcgt ccatggctat gccgccatgt gtcttctcgc cgtcggtctt ctgggacgat
     4141 cgctggccgt gcccgcacaa gagtatctgc tgctgtcggg gcggaaccgg gtcctgacac
     4201 ggatcggggc tatcacgctc gcggtccttg ccgtgctgtg cgccgtcctg gcgccggcct
     4261 tcggcctcac gggggccgca accgccgcag ccattgccca gctgctgcgc agcctcctcc
     4321 tcatccgcgc cacccggcgc atgggcggcc tgctggtcct gccgccgctg cgacttggac
     4381 ggcagcgctt cattgccgcg cccggcggtt cgcagtaagt cttcggcggg atcgatcagc
     4441 gaggagacac ggcatggacc ggttcatcgg cggctgcctg tgcggcgatg tgcgcttcgt
     4501 ggcgaccggc cgcccctatc gcgtcggact atgccactgc atggactgcc gcaagcatca
     4561 cggcgcagtg ttccatgcct ccgcgatctt ccccgagagc gccgtgagcg tggaaggcgg
     4621 gacgcgcgac tattgcggac ggcatttctg cccgcgctgc ggttcttccg tcttcgcccg
     4681 gacgggcgac gaagtggagg tgaacctcgg ctcactggac acgcccgacc agctgcggcc
     4741 gacctacgaa agctggacca gccgccgcga atcctggctg ccaccgtttc cgctcaagcg
     4801 gcgatacgag cgcgatcgcg atgctaccgg acggttcgaa gactgacgga tctcggcatg
     4861 gtgagctgtg ccggatcagc cgtccctgcc gaggacatgc tgcagattca gcgcccttct
     4921 cacgctggcc aacggggcct tgaggacaac cgcctcccgg tcccagatgc cgtaggacag
     4981 gaaaatcgag cggccggaga cggccagccc gcaacagaac tcgaccttct gaccctcgaa
     5041 ggagaatggc acgctcacgg cggccacatc gaagttcgac cgatacacca ccaggctgtg
     5101 cgtataggtc ctcagccgac cgctggccgg tgcgccgcgg acggcatctc gatgctggcg
     5161 gtggatgatg ccgatgaacc cgtcctgcca gggaatgacg caggtcccgc cgaactggcc
     5221 ctccagggcc tggtgcgacg ccgggtggag cgactgctgg ctcttcccct tctccagatg
     5281 cagcacttcg tagggatcct gcgcatagat cgcgaagaga tcgtcgcccc ggacccaggg
     5341 catccagttc ttctcccgca attggcgcga cggcaacacc gcggcaacct tcagggtctt
     5401 gcccgccagc cggcagagga cgacgcgctc gaccagacgg cggcgcaccg ctccggattg
     5461 ggtctcgtgg gtggcggccg cccccatgca ccacaggcca tcgcgccacg cgaagagccg
     5521 gagatcctgc agtccgtgca gcgcaaaggg gcgcgcccgg atgtgcctgt cctcgaggaa
     5581 gtgggcgccg cggaccttga ggtccatgtc cagctccgcc acgtagttct ggctgtcgca
     5641 gaagggcacg ttgtagccgc cataggtgaa gcggtagccc tccgtctcga gatcgaagtt
     5701 gatgcccttg tagacacaga ggattgtgtc gtccctgacg atcagcgaag gattgcaggg
     5761 cttgtgggcc gagagcggcg gcaggcttgc ccgggcctcg acctcgatcg attcatcaag
     5821 ccgcaacacg atctcaccct tgcccgaccg cttccggacg cccacgtccc ggaaccgaaa
     5881 aacaattgca tttcacggag ggggaaagca agctggcacg cgtccagccg cccgccgcaa
     5941 gatacaggga gtagaatcct ttgggtaatc ag
//
LOCUS       DBNGVR010000182         2562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_459709, whole genome shotgun sequence.
ACCESSION   DBNGVR010000182 DBNGVR010000000
VERSION     DBNGVR010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_459709"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..2363
                     /locus_tag="AC16A0_03975"
     CDS             240..2363
                     /locus_tag="AC16A0_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3104519.1"
                     /translation="MLLLLAAVTIFSAAPKAQTETSSPPAKVEELLQLLGDPEVQKWL
                     DAQKAAKAATAPANPVAQEKMAIEANFERIRRHLASLAAAIPTFPQELGRVLATVHGD
                     MMGYGLLRVLAIFAAFAAAGVLAQWLFWRLAAGLRAWIASANLATVQDRLIAMFARLA
                     YSAGSVLAFAAGSIGAFLAFGWPPRLHQIIIAYLVAVLIYRSARVLLEFLLSPPDLKG
                     FGDSARFRVLPVSDARASYWVRRFSLAIGWYAFGSATVGLLGALGLSLPARQLVAYAL
                     GLVLLAIGAEAVWNRPVDPRAAGSPAAETWFNAKARTYLLILSFFILWVLWVESATWA
                     FWLMAVFLALPLAITAARNAVNNVLRPAGSAPGGNEIPSVIAAVVERGLRVALVAAAV
                     VFLANRLGIDFTTMTAQDTVGTRLMRGALSATIILLTADFVWHVTKTVIERKIAESAS
                     MGAAGSDLARRRDRIRTLLPIARNVLMIVIAVVAILMALSSLGVEIGPLIAGAGILGV
                     AIGFGAQTVVKDIISGVFYLLDDAFRVGEYIQSGSYKGTVESFSLRSVKLRHHRGSIY
                     TVPFSDLGAIQNMSRDWAIDKITLNITYDSDVDLARKIVKKIGLELAQDPEFAAATLQ
                     PLKMQGIDEFGESGLMLRMKIMTRPGEQFTIKRRALMMIKKAFDENGIKLAVPTVQVS
                     GDSDPKTAAANQLRRKKQQAAAAPA"
BASE COUNT          416 a          879 c          807 g          460 t
ORIGIN      
        1 gttactcgtt gccagcgaag gtccagcaac accaactcgc aggagcggca cgatcatcac
       61 catggtcgtg tcaaaccgtc cgcccggtca aacctgcagg cccacgggtt tgagtgccag
      121 tccctgcctc aagatgactt ggccacccgc atgcgataag acaatggaaa atcggagggg
      181 cagtcttgca catctggacg gaatctcact ttgcggcggt caagcggggc attcttgcga
      241 tgctcttgct cttggcggcg gtgaccattt tttcagccgc acccaaggca cagaccgaga
      301 cgtccagtcc tccggccaag gtggaagaac tgctgcagct cctcggcgat cccgaggtgc
      361 agaagtggct ggacgcccag aaggcggcaa aggccgcgac ggcgccggcc aatcccgtcg
      421 cgcaggagaa gatggcgatc gaggcgaact tcgaacgcat ccgccggcat cttgccagcc
      481 tcgctgcagc gattccgaca tttccgcagg aactcgggcg ggtgctggcc acggtgcacg
      541 gcgacatgat gggctatggg ctcttgcgcg tcctcgcgat cttcgccgcc ttcgccgccg
      601 ccggcgtgct ggcgcagtgg ctgttctggc gcctggcagc agggcttcgc gcctggatcg
      661 ccagcgccaa tctcgcaacg gtgcaggacc ggctcatcgc catgtttgcg cgccttgcct
      721 acagcgcggg ttcggtgctt gccttcgcag caggaagcat cggcgccttt ctcgccttcg
      781 gctggccgcc gcggctgcat cagatcatca tcgcctacct cgtcgccgtc ctgatctacc
      841 gctcggcgcg cgtgctgctg gaattcctgt tgtcgccgcc ggacctcaag ggcttcggcg
      901 actcggcccg cttccgtgtc ctgccggttt ccgatgcgcg cgccagctac tgggtgaggc
      961 gcttcagcct ggccatcggc tggtatgcct tcgggagtgc cacggtgggc ctgcttggcg
     1021 cactgggcct ctcgcttcct gcccgccaat tggtcgccta tgccctcggc ctcgtcctgc
     1081 tcgcgatcgg cgccgaggcc gtctggaacc ggccggtcga cccccgggcg gccggcagcc
     1141 ccgccgcgga aacatggttc aatgccaagg cgcggaccta tctcctgatc ctgagcttct
     1201 tcattctctg ggtgctgtgg gtggaatccg ccacatgggc cttctggctc atggccgtct
     1261 tcctcgcctt gccgctcgcc atcacggccg cccgcaacgc agtgaacaac gtgctgcggc
     1321 cggcaggatc ggcgcccggc gggaacgaga tccccagcgt catcgccgcc gtcgtcgaac
     1381 gcggcctgcg ggtggccctg gtcgccgccg ccgtggtctt tctcgcgaac cggcttggaa
     1441 tcgacttcac caccatgacg gcccaggaca cggtcggtac ccgcctgatg cgcggggccc
     1501 tgagcgcgac catcatcctc ctcaccgccg atttcgtctg gcacgtcacc aagaccgtca
     1561 tcgagcggaa gatcgccgaa tccgccagca tgggcgcagc cggatcggac ctcgcccgcc
     1621 gccgcgaccg gatccgtacg ctcttgccga tcgcccgcaa tgtgctgatg atcgtcattg
     1681 ccgtcgtcgc catcctgatg gccctgtcgt cgcttggcgt cgagatcggc ccgctcatcg
     1741 ccggcgcagg cattctcggc gttgcaatcg gttttggcgc gcagaccgtc gtgaaggaca
     1801 tcatcagcgg tgtcttctat ctgctcgatg atgccttccg ggtgggcgaa tacatccaga
     1861 gcgggtccta caagggaacc gtcgagtcct tcagcctcag gtcggtgaag ctgcgccatc
     1921 accgcggctc gatctatacg gtgcccttca gcgacctggg cgccatccag aacatgagcc
     1981 gcgactgggc catcgacaag atcacgctca acatcaccta tgactccgac gtcgacctgg
     2041 cgcgcaagat cgtgaagaag atcggtctcg agctggccca ggacccggaa ttcgctgccg
     2101 ccaccctgca gcctctcaag atgcagggca tcgacgagtt cggcgagtcg ggcctgatgc
     2161 tgcggatgaa gatcatgacc cggccgggcg agcaattcac catcaagcgc cgcgcgctga
     2221 tgatgatcaa gaaggccttc gacgagaatg gcatcaagct ggccgttccg acggtgcagg
     2281 tctccggcga ctccgacccc aagacggccg ccgccaatca gctgcgccgg aagaagcagc
     2341 aggccgccgc agcgccagcc tgacacgctg tgggacgggg cttgcctggc gccggtccag
     2401 cgggaatgat gccgtggatg agcgacgccc aaaccgaaag cacgcggaag caggccgtgg
     2461 cggacccgtc cgccagcgac gccctgcttg ccagcctcta ccgttcggcg cgtgcggcgg
     2521 ccgatggccc gggcctggtg tggaacgcac aggtcgggtg ct
//
LOCUS       DBNGVR010000183         2339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1836185, whole genome shotgun sequence.
ACCESSION   DBNGVR010000183 DBNGVR010000000
VERSION     DBNGVR010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1836185"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..280)
                     /locus_tag="AC16A0_03980"
     CDS             complement(23..280)
                     /locus_tag="AC16A0_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104520.1"
                     /translation="MKRIAAVLVALAPALPDAALAQDTDVSIIAAQVRSQGFACDNPT
                     GAERVVDESAPDQPVYTLACDGVTYRVKLIPDMAAEITKID"
     gene            complement(277..912)
                     /locus_tag="AC16A0_03985"
     CDS             complement(277..912)
                     /locus_tag="AC16A0_03985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR3104521.1"
                     /translation="MLTHKQVWYAIDVIAERYGFSASGLAKKSGLDPTSFNPSKRHGP
                     DGRPRWPTMESISRLLQASGATMEEFADLLIGRKNAPARRKHIPVLGFAKAGKGGFFD
                     DSGFPAGNGWDEIEVPGVTDSNAYALEITGDSMMPVYREGDTIVISPAATIRKGDRVV
                     VRTTDGQVMAKIMQRQTSKTIELASFNADHPPKLLDMKDVDWVARIVWASQ"
     gene            1012..1353
                     /locus_tag="AC16A0_03990"
     CDS             1012..1353
                     /locus_tag="AC16A0_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HIR3104522.1"
                     /translation="MRFLFKICPRGDWALAEAAGHYAGSAVDHRDGFIHLSSDAQVRE
                     TATRHFAGQEGLVLVALPVDGLDGLRWEPSRGGALFPHVYGTIPVTKALWVRDLPLVD
                     GLHVFPPEAGA"
     gene            1350..>2339
                     /locus_tag="AC16A0_03995"
     CDS             1350..>2339
                     /locus_tag="AC16A0_03995"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR3104523.1"
                     /translation="MSLAGRAFPLLRPLLHSLDAETAHGLTIRLLRGFAPGTPRSSDR
                     SLAVAAFGLAFPNPLGLAAGFDKNAEVPDAMLALGFGFVEIGTVTPRPQAGNPRPRLF
                     RLPADRGVINRMGFNNEGQAATLRRLEARAGRPGILGVNIGANKDSADRVADYVAGIE
                     AFSHLASYVTINISSPNTPGLRGLQSRAELETLLSRLGEARARQRRQPPMLLKIAPDL
                     GDDELEDIARCCGGGAVDGIIVSNTTLRRDGLASPARGEAGGLSGAPLFALSTRVLAK
                     VYLLTGGRLPLIGAGGIGDAETAWIKLRAGATLLQLYSALVYAGPALVEEILTG"
BASE COUNT          340 a          785 c          788 g          426 t
ORIGIN      
        1 ggcaaacccg gaactcgggc cgctagtcga tcttggtgat ctccgcggcc atgtccggaa
       61 tgagcttcac gcgataggtc acgccgtcgc aggcgagcgt gtagaccggc tggtcgggcg
      121 ctgattcatc gacgacgcgt tcggcgcccg tcggattgtc gcaggcgaag ccctggctgc
      181 gcacctgggc ggcgatgatc gagacatcgg tatcctgcgc cagggcagca tccggcaggg
      241 caggtgccag cgccacgaga acggcagcga tcctcttcat tggctggccc agacgatgcg
      301 ggcgacccag tcgacgtcct tcatgtccaa cagctttggc ggatggtcgg cattgaagga
      361 ggccagctcg atggtcttcg aggtctggcg ctgcatgatc ttggccatga cctggccatc
      421 ggtggtgcgc accaccacgc ggtcgccctt gcggatggtg gcggccggcg aaatcacgat
      481 cgtgtcgccc tcgcgataga ccggcatcat cgagtcgccg gtgatctcca gcgcataggc
      541 gttgctgtcg gtgacgcccg gcacctcgat ctcgtcccag ccattgccgg ccggaaagcc
      601 ggaatcgtcg aagaagccgc ccttgcctgc cttggcgaag cccagcaccg gaatgtgctt
      661 gcgccgcgcc ggcgcgttct tgcggccgat cagcaggtcg gcgaactcct ccatcgtggc
      721 gcccgaggcc tgcagcagcc gcgagatgct ctccatggtc ggccagcgcg gacggccgtc
      781 cggcccgtgg cgcttggacg gattgaagga ggtcgggtcg aggcccgatt tcttggccag
      841 gcccgaggcg gaaaatccgt agcgctcggc gatgacgtcg atggcgtacc agacctgctt
      901 gtgggtgagc atgttcggtc ctgtctgctg ctaagctgct taagcgatag gaacctatac
      961 ctcatgcctg ggcgattgtc catggcgcgc ctgccgccta aggtccgtgc catgcgattc
     1021 ctgttcaaga tctgcccgcg cggcgactgg gccctggccg aggctgccgg gcactacgcc
     1081 ggttccgcgg tggaccaccg cgacggtttc atccatctct cctccgatgc ccaggtgcgc
     1141 gagaccgcta cgcgccattt cgccgggcag gagggactgg tactggtggc cctgccggtg
     1201 gacggcctcg acggcctgcg ctgggaaccc tcccgcggcg gcgcgctctt tccgcatgtc
     1261 tatggcacga tcccggtgac gaaggcgctc tgggtaaggg atctacctct ggtagacggt
     1321 ctgcacgtct ttccgcccga ggctggcgca tgagcctggc aggccgggcc tttcccctgt
     1381 tgcggccgct gctgcacagc cttgatgccg aaaccgcgca tggtctcacc atccgtctgc
     1441 tgcggggctt tgcgccggga acgccgcggt cctccgatcg cagcctggcc gtcgcggcct
     1501 tcggcctcgc atttcccaat cccctgggac ttgcggcggg cttcgacaag aatgccgaag
     1561 ttcccgatgc catgctggcg ctgggtttcg gtttcgtgga aatcggcacg gtcacgccca
     1621 ggccgcaggc gggcaatccg cgcccccgcc tgtttcgcct gcccgccgac cgcggcgtca
     1681 tcaatcgcat gggtttcaac aatgaaggcc aggccgccac gctgcgccgg ctcgaggcgc
     1741 gcgccggccg gcccggcatt ctgggcgtga acatcggcgc caacaaggac tctgccgacc
     1801 gcgtcgccga ctatgtggcg gggatcgagg ccttctcgca cctggcgtct tatgtgacga
     1861 tcaacatctc ctcgcccaat acgccgggct tgcgcggcct gcagtcgcgc gccgaactgg
     1921 aaacgctcct gtcccgtctg ggcgaggcgc gggcgcggca gaggcggcag ccgccgatgc
     1981 ttctcaagat cgcgcccgat ctcggcgatg acgaactcga agacattgcc cgctgctgcg
     2041 gcgggggagc cgtcgatggc atcatcgtgt ccaataccac cctgcgccgt gatgggcttg
     2101 catcgccggc ccggggcgag gcgggcggcc tgtcgggtgc accgctcttt gcactgtcga
     2161 cccgtgtgct ggccaaggtc tatctgttga ccggcggcag gctgccgctg atcggtgccg
     2221 gtggcatcgg cgacgcggag acggcctgga tcaagctgcg cgcgggtgcc accctgctcc
     2281 agctctactc ggccctggtc tacgccggcc ctgcgctagt ggaggaaatc ctcacgggg
//
LOCUS       DBNGVR010000184        10922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3800900, whole genome shotgun sequence.
ACCESSION   DBNGVR010000184 DBNGVR010000000
VERSION     DBNGVR010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3800900"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /gene="queG"
                     /locus_tag="AC16A0_04000"
     CDS             complement(<1..1094)
                     /gene="queG"
                     /locus_tag="AC16A0_04000"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR3104524.1"
                     /translation="MGGLAEDLKARARAEGFDDVRITALRDDPVRAGRLTEFLASGHH
                     GSMDWMAETAPRRSSPLTLWPAARSAVVLAQSYAPDTDPMARLHDRDSGVISVYALNR
                     DYHDVVKGKLKRLGQWLAHRSGAEVKVFVDTAPLMEKPLAADAGLGWQGKHTNLVSRQ
                     LGSWFFIGTILTAAALPPDQPEADHCGTCRACLDICPTRAFPAPYRLDARRCISYLTI
                     EHKGHIDRGFRKAMGNRIYGCDDCLAVCPWNKFAQAASEAKLVARDDLRAPALSRLLQ
                     LDDAAFRKLFSGSAVKRIGRDRFIRNVLIAAGNSGREDLIADVAPLLSDAAPVVRAMA
                     VWAMSQLASPDAFARLRAQHRGIESDPDVLL"
     gene            complement(1097..1780)
                     /locus_tag="AC16A0_04005"
     CDS             complement(1097..1780)
                     /locus_tag="AC16A0_04005"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3104525.1"
                     /translation="MPALIHFALDPFSRRMRLALAEYGVGVDFIEETPWDPSHDLFEL
                     NPAGTVPVYLEDSTSPVSGVEAIGEFLEETKSARIKLIPGNAFARAEVRRIVGWFDVK
                     FYAEVSEPLITERVIRRYLPRENGGGGPDMGRVRQAAQRLREHLAYIGHLADHHPWLA
                     GEALSLADLAAAAHVSVADYFGDVPWSDYPSAKSWYQRIKSRPSFRPLLADTVRGMAP
                     SANYADLDF"
     gene            1941..2765
                     /locus_tag="AC16A0_04010"
     CDS             1941..2765
                     /locus_tag="AC16A0_04010"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3104526.1"
                     /translation="MVILQIVVLALIQGITEFLPISSSGHLLLVPALTGWPDQGVLTD
                     VMVHMGSFLAVVVYFWRDCVNLTLGALDFLRGRITPWGKLAMLIVLGTIPAVVFGVIL
                     DKIGFMDAVRQMPQIIAWNAIIFGILLYLCDRFGLSIRRMSDMTLAPALIIGVAQAIA
                     IIPGTSRSGITMTAARALGFERPEAARFAFLLGIPAIAGAGVFKLGDAAASGEAISFG
                     MLLTAVLTFFVALGTITVLMKLVRHMSFLPFAIYRVLLGLVLLALIYSGMPLGAVN"
     gene            complement(2732..3718)
                     /locus_tag="AC16A0_04015"
     CDS             complement(2732..3718)
                     /locus_tag="AC16A0_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIR3104527.1"
                     /translation="MLVNQQNAEKLITIIGGSGFLGRHIVRALAKRGYRIRAACRRPD
                     LAGHLQPLGVPGQVMPVQANVRYPASLAAACDGAYAVINLTGVLYSAGAQSFDAIHAF
                     GAEASAKAARAARARVFIQMSAIGADPGSSAAYARSKAEGEARARAAFPGAIILRPSI
                     VFGPEDSFFNRFAGLARFAPALPLIGGGATKFAPVFVGDVAEAVARLVDRGEADGKTY
                     ELGGPEVLTFKQLMQFTLNTIERNRLLIPVPWNVARLQGLILGLLPNPLLTADQVELL
                     KSDNVVSEAAQREKRTLDGLGIAAQGIEGIVPGYLYRFRKAGQFTAPKGMPE"
     gene            3876..3960
                     /locus_tag="AC16A0_04020"
     tRNA            3876..3960
                     /locus_tag="AC16A0_04020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Leu,seq:cag)
     gene            3992..4603
                     /locus_tag="AC16A0_04025"
     CDS             3992..4603
                     /locus_tag="AC16A0_04025"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR3104528.1"
                     /translation="MAIKLYKGDLPAGLDFGSSVAIDTETLGLNPRRDKLCLVQLSSG
                     DGNAHLVQLDRSAYDAPHLKALLTNPNVTKIFHYARFDVAALKHHLGVATAPVYCTKI
                     ASKLTRTYTDRHGLKDLIKELLGIDLNKQQQSSDWGAHVLSDAQKQYAAQDVIYLHEV
                     KARLDQMLAREGRTGLASACFDFVPVRADLDLAGWTEEDVFAH"
     gene            4644..5246
                     /gene="lptC"
                     /locus_tag="AC16A0_04030"
     CDS             4644..5246
                     /gene="lptC"
                     /locus_tag="AC16A0_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR3104529.1"
                     /translation="MRFASTARLLSYLAGAIGVGAFLLFLFQAGLFSAFMPKEKLNVP
                     VIENPDSISAQTSTVTGLDRQNQPYEVTAKRGRQDAVKPNIVYLEDIEAVFRKPGGKV
                     YNVTSASGVYDDKAKDVNLEGQVVISEATTFTARMEKAYVQIETKAMTSDVPVTVDMV
                     GGGTIAANGMQITDDGTKILFLNGVKARFGAETGKGDQTP"
     gene            5243..5734
                     /locus_tag="AC16A0_04035"
     CDS             5243..5734
                     /locus_tag="AC16A0_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR3104530.1"
                     /translation="MTLRRILVAGALALAAFGSGALAQESISSGPQATDIEADQMEVV
                     DAEKKAIFKGNVNAKRGKVTLKSAELVVHYADVKQADGTTKTDATNIDATGNVVIVTA
                     KETITGDWAKIDVQSDKLVVGGNVKLVQGSTILTGPELRADLKSNRMEMKGGRVKGSF
                     LPK"
     gene            5774..6574
                     /gene="lptB"
                     /locus_tag="AC16A0_04040"
     CDS             5774..6574
                     /gene="lptB"
                     /locus_tag="AC16A0_04040"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR3104531.1"
                     /translation="MARDDNVKVARKPARTRKKVPEFHDGNEGLVVEGLSKTYKRRPV
                     VRNVSLSLRRGEAVGLLGPNGAGKTTVFYMITGLIAADSGSIRLDGADITHLPMYRRA
                     RLGIGYLPQESSIFRGLTVEENIRAVLELTESSRSRRDSMLRELLDEFSITHLRNSPA
                     VALSGGERRRVEIARALAASPQFMLLDEPFAGIDPIAISDIRKLVRQLTARGIGVLVT
                     DHNVRETLELIDTALIIHEGQVLTVGTPDEIVRNPDVRRHYLGADFSM"
     gene            6595..8106
                     /gene="rpoN"
                     /locus_tag="AC16A0_04045"
     CDS             6595..8106
                     /gene="rpoN"
                     /locus_tag="AC16A0_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR3104532.1"
                     /translation="MAIGPRMEMRQGQSLVMTPAMQQAIKMLPMSNIDLQAFLESELE
                     RNPLLELDDGSEVREAPASHQADTVPEVSADPPPEDGYAGYDGEADGRSGGAGEPVIA
                     GPGGLGPQDSNWSSVRSGGGAGFDGDDMEFASTISREETLVEHLSQQLSLAISDPADL
                     LIGQHIIGMVNEAGYLTGDTAGIADMLGTSVAHVDRVLAVMQQFDPPGVFARDMRECL
                     MLQLIDRNRYDPAMAKLLDNLKLLGERNYAALRSICEVDRDDLAEMIDEIHRLEPKPG
                     RQFGSDPVQPIIADVIVRGAPDGSWIVELNSETLPKVLVHNSYAARVSKSSTREEDKL
                     FISKMSEYQAHAKWLVESLDHRANTILKVAREIVRQQDAFLVHGVQQLRPLNLRNVAD
                     AIGMHESTVSRVTSNKYMATPRGIFEMKFFFTRAIAATESGDEAHSAEAVRHRIRELV
                     AAEGEAVLSDDDLVGKLKQEGIEIARRTIAKYRESLGIRSSVQRRREWREKHR"
     gene            8308..8880
                     /gene="hpf"
                     /locus_tag="AC16A0_04050"
     CDS             8308..8880
                     /gene="hpf"
                     /locus_tag="AC16A0_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR3104533.1"
                     /translation="MQIKITGKNLDVGSALRDHVEAKLLQLKSKYFEGTVHGHVTVEK
                     QRGNFHTDCTLHLATGLTLQATGDAAEAHPSFDAAANHIEKQLRRYKRRLKDHHKSRR
                     EPVRHLEAASYVITPAPEEEDEAAGLNPVVVAEADARLPELSVGEAVMQLDISNVPFV
                     LFRNGGHGRLNVVYRRKDGNIGWVDPKPAA"
     gene            8916..9392
                     /gene="ptsN"
                     /locus_tag="AC16A0_04055"
     CDS             8916..9392
                     /gene="ptsN"
                     /locus_tag="AC16A0_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HIR3104534.1"
                     /translation="MSLDEIIDDQSVLANVKAQGKKQLLLELAQAMASRVAIDHRVIF
                     ETLLTREKLGSTGIGQGIAIPHGKVPTLNRVYGLFARLATPIDFESVDGQPVDLVFVL
                     LAPDHAGADHLKALAKISRLLRDPVMVQKLRGTESAESLYAILTEPAATASSATAA"
     gene            complement(9406..9669)
                     /locus_tag="AC16A0_04060"
     CDS             complement(9406..9669)
                     /locus_tag="AC16A0_04060"
                     /inference="COORDINATES: protein motif:HMM:NF018343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HIR3104535.1"
                     /translation="MNTQDTKKTLSLSESDLAKLGGGALAYIREIKGKDARKMLGGQA
                     TIPANARLFCLFHADGTPVSISPSREAAVGSAFEHELMPMSVH"
     gene            complement(9675..10097)
                     /locus_tag="AC16A0_04065"
     CDS             complement(9675..10097)
                     /locus_tag="AC16A0_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HIR3104536.1"
                     /translation="MSRVSMFSSPFLLGFDELERMLDRASKTSGEAYPPYNIERLPRA
                     EGEAERLRITLAVAGFSRDELEVTVEENQLVIKGRQREDQDRSYLHRGIAARQFARTF
                     VLADGMNVTAARLENGLLIVSLERPEPRRVVRRIEIGG"
     gene            10302..>10922
                     /locus_tag="AC16A0_04070"
     CDS             10302..>10922
                     /locus_tag="AC16A0_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3104537.1"
                     /translation="MKRRNLLAAVAAVAMSATAAFAADDKPAIVFDIGGKFDKSFNES
                     MYNGSEKFKAETGIAYVVLEIANEAQREQAIRNFADQGYSPIIAAGFAQAAAVEKVAK
                     EYPELKFAIVDMVVDLPNVQSIVFKEHEGSYLVGMLAGMASKSGKVGFVGGMDIPLIR
                     KFACGYAQGVKAAKADATIFQNMTGDTGAAWNDPVKGGEITKGQMAQ"
BASE COUNT         2063 a         3438 c         3504 g         1917 t
ORIGIN      
        1 agcagcacat cgggatcgct ctcgatgccg cgatgttgcg cgcgcagtct ggcaaatgca
       61 tccggcgatg caagctgcga catggcccag accgccatgg cgcgcaccac cggtgcggca
      121 tcggacagca ggggcgcgac atccgcaatc aggtcttcac ggccgctgtt acccgccgca
      181 atcagcacat tgcggatgaa ccggtcacgg ccgatgcgct tgaccgcgga gccgctgaac
      241 agcttgcgga atgccgcatc gtcgagctgc agcagccgcg acagggcagg ggcgcgcaga
      301 tcgtcgcggg ccacgagctt tgcttcggat gcggcctgcg cgaacttgtt ccacgggcag
      361 acggcaaggc aatcgtcgca gccatagatg cggttgccca tcgccttgcg gaagccgcga
      421 tcgatgtgac ccttgtgctc gatggtcaga taggagatgc agcggcgggc gtcgagccgg
      481 tatggtgccg gaaaggcccg ggtgggacag atgtcgaggc aggcgcggca ggttccgcaa
      541 tggtcggcct ccggctgatc gggcggcagc gcggcggcgg tcaggatggt gccgatgaag
      601 aaccacgatc ccagctggcg cgaaacgaga ttggtgtgct tgccctgcca gcccagaccc
      661 gcatcggccg ccagcggctt ctccatcagc ggcgccgtgt cgacgaagac cttgacctcg
      721 gcgccggagc ggtgcgccag ccattgcccc aggcgcttga gcttgccctt gaccacgtca
      781 tggtagtcgc gattgagggc gtagacggaa atgacgccgg agtccctgtc atgcaggcgt
      841 gccatcgggt ccgtgtcggg cgcatagctc tgcgccagga cgacggcgct gcgggcagct
      901 ggccacaagg tgaggggact ggagcggcgc ggggccgtct cggccatcca gtccatgctg
      961 ccatgatggc cgctggcgag gaattccgtc aggcggccag cgcgtaccgg atcgtcgcgc
     1021 agtgcagtga tgcggacgtc atcgaagcct tcggccctcg cccttgcctt cagatcctcg
     1081 gcgagcccgc ccacgcctag aaatcgaggt ctgcgtagtt tgccgatggc gccatgccgc
     1141 gcaccgtgtc ggccagcagc ggccggaagg atggccgcga cttgatacgc tgataccacg
     1201 acttggcgga aggataatca gaccacggaa catcgccgaa atagtcggcc accgagacat
     1261 gggccgccgc ggcgagatcg gccagcgaca gggcctcgcc ggcaagccag gggtggtggt
     1321 cggcaagatg gccgatatag gcgagatgtt cgcgcagccg ctgcgcggcc tgccgcaccc
     1381 ggcccatgtc gggtccgccg ccgccgttct cgcgcggcag gtaacggcgg atcacgcgct
     1441 cggtaatgag aggttccgag acctcggcat agaacttcac gtcgaaccag cccacgatgc
     1501 ggcgcacctc ggcgcgcgcg aaggcattgc ccggtatgag cttgatgcgg gcggactttg
     1561 tctcctcgag aaactcgccg atcgcctcga cgcccgacac cggcgatgtg ctgtcctcga
     1621 gatagacggg gaccgttccg gcggggttga gctcgaagag atcatggctg ggatcccacg
     1681 gcgtctcctc gatgaagtca acgcctacgc catattcggc aagcgccagc cgcatgcggc
     1741 ggctgaaggg atcgagcgca aaatggatga gtgccggcat ggtctgcgaa ggtgctttcg
     1801 gtttcaggct gctggcgcgg ggcacgtgcc gccgcgattg ccccgccgct gcgctgcata
     1861 taaaacccgg gcgtccctac agcaagcgat tcgagttagc gcaggccggg ccgtcagacc
     1921 ggagcaggaa tgtcagtttc atggtgatac tccagatcgt cgtcctcgcc ttgatacagg
     1981 gcatcaccga attcctgccg atctcctcgt caggccacct gcttctcgtt cctgccctga
     2041 ccggctggcc cgaccagggc gtgcttaccg acgtgatggt gcacatgggg tcctttctcg
     2101 ccgtcgtcgt ctatttctgg cgcgattgcg tcaacctgac ccttggcgcg ctggactttc
     2161 tccggggccg catcacgccc tggggcaagc tggccatgct gatcgttctg ggcaccattc
     2221 ccgccgtggt gttcggcgtc atcctcgaca agatcggctt catggatgcg gtccgccaga
     2281 tgccgcagat catcgcctgg aacgccatca tcttcggcat ccttctttat ctctgcgacc
     2341 gcttcggcct gtcgatccgc cgcatgtcgg acatgacact ggcgcccgca ctgatcatcg
     2401 gcgtggcgca agccattgcc atcattcccg gcaccagccg gtcgggcatc accatgacgg
     2461 cagcccgggc gcttggcttc gaacggccgg aggccgctcg cttcgctttc ctgctcggca
     2521 ttccggccat tgccggtgcc ggcgtcttca agcttggcga tgccgccgca tccggtgagg
     2581 cgatctcctt cgggatgctg ctgaccgcgg tcctgacctt cttcgtggcg ctgggcacga
     2641 tcaccgtact catgaagctt gtgcgccaca tgagcttcct gcccttcgcc atctaccgcg
     2701 ttctgctggg cctcgtcctg ctggcgctga tctattccgg catgcccttg ggcgcggtga
     2761 actgacccgc cttgcggaac cggtagagat agccggggac gattccctcg atgccctgcg
     2821 ccgcgatgcc caatccatcc agcgtgcgct tctcgcgctg cgccgcctcg ctcacgacat
     2881 tgtcggactt cagaagctcg acctggtctg ccgtgagcag tggattgggc agcaggccca
     2941 ggatcaggcc ctggaggcgg gcgacgttcc agggaaccgg aatcagcagc ctgttgcgct
     3001 cgatggtgtt gagggtgaac tgcatcaact gcttgaaggt gagcacttca ggcccgccaa
     3061 gttcgtaggt cttgccatcc gcctcgcccc tgtcgaccag ccgcgccacc gcctcggcca
     3121 cgtcgccgac gaagaccgga gcgaacttcg ttgccccgcc gccgatcagc ggcaaggccg
     3181 gcgcgaagcg ggcaaggccg gcgaaccggt tgaagaagct gtcctccggc ccgaagacga
     3241 tggacgggcg caggatgatg gcacccggaa aggcagcccg cgcgcgggcc tcgccttcgg
     3301 ctttgctccg ggcataggcg gccgaactgc cgggatcggc gccgatggcc gacatctgga
     3361 tgaagacacg cgcccgcgcc gccctggcgg cctttgcgga ggcctccgcg ccgaaggcat
     3421 ggatggcatc gaagctctgg gctccggcgg aatagagcac gccggtcagg ttgatcaccg
     3481 cataagcccc gtcgcaggcc gccgccagcg atgccgggta gcgcacattg gcctgcaccg
     3541 gcatgacctg gcccggcacg ccgaggggtt gcaggtggcc ggccagatcg gggcggcggc
     3601 aggctgcccg gatgcgatag ccccgcttgg ccagcgccct gacgatgtgg cggccaagaa
     3661 agccggagcc gccgatgatg gtgatgagct tttccgcgtt ctgctggttc acgagcatgg
     3721 catccagtga ttcagttgag cgggttctta gcgcccgcgc tgcgccgatg caaagccgat
     3781 gtcgccgccc gtgccgtcag gacgcgaagc cgcggcgggc ggctcaagcg gtttggttga
     3841 caggccccgc aacccacact aagagacccc ccgaagccca gatggcggaa ttggtagacg
     3901 cactagtttc aggtactagc gctgaaaggc gtggaggttc gagtcctctt ctgggcacca
     3961 tctttcgaca gtcgcagcgt caggatcccc gatggccata aagctttaca agggcgacct
     4021 gcccgccgga ctggatttcg ggtcctcggt cgcgatcgac accgagacgc tcggactcaa
     4081 tccccgccgc gacaagctct gtctcgtgca gctgtcgtcg ggcgacggca atgcccacct
     4141 ggtgcagctt gaccggtctg cctacgatgc gccgcatctc aaggcgctgc tgaccaatcc
     4201 gaacgtcacc aagatcttcc attacgcacg gtttgatgtt gccgcgctga agcaccatct
     4261 cggcgtggcg acggccccgg tctattgtac caagatcgca tccaagctga cccggaccta
     4321 taccgaccgc catggactga aggacctgat caaggaactt ctcggcattg acctgaacaa
     4381 gcagcagcag agctccgact ggggcgccca tgtgctgagc gacgcccaga agcagtatgc
     4441 ggcgcaggac gtgatctatc tgcatgaagt caaggcgcgg ctggatcaga tgctggcccg
     4501 cgagggccgt accggacttg ccagcgcctg cttcgacttt gtgccggtgc gcgccgatct
     4561 cgatcttgcc gggtggaccg aggaagatgt cttcgcccac tgaaacaagt ggcagaaccg
     4621 ataacggccg tgcggcacgg gccgtgcgct ttgcttcgac ggcgcgcctt ctgagctatc
     4681 tggcaggtgc catcggcgtc ggggcatttc tgctcttcct gttccaggcc gggctttttt
     4741 cggctttcat gccgaaggaa aagctcaacg tgccggtgat cgagaatccc gactcgatat
     4801 cggcccagac ctccaccgtc accgggctgg accggcagaa ccagccctat gaagtgacgg
     4861 ccaagcgcgg ccgtcaggat gcggtgaagc caaacatcgt gtatctcgag gacatcgagg
     4921 cggttttccg gaagcccggc ggcaaggtct acaatgtaac ctccgcatcg ggcgtctatg
     4981 acgacaaggc caaggacgtg aatctcgagg gccaggtcgt gatcagcgag gcgaccacct
     5041 tcaccgctcg catggaaaag gcttacgtgc agatcgagac taaggcgatg acgtcggacg
     5101 tgccggtcac ggtcgacatg gtggggggcg gcacgattgc cgccaacggc atgcaaataa
     5161 ccgatgacgg aacgaagatt ctgttcttga atggcgtgaa agcgcgcttc ggcgcggaaa
     5221 ccggaaaggg agaccagacc ccatgactct acggcggatt ttagtggcgg gcgccctggc
     5281 cctggccgct ttcggttcgg gggccctggc gcaggagagc atcagctcgg gtccgcaagc
     5341 aaccgatatc gaagcggatc agatggaagt ggtggacgcc gagaagaagg cgatcttcaa
     5401 gggcaacgtg aatgccaagc gcggcaaggt gacgctcaag agcgcggaac tcgtggttca
     5461 ctacgccgac gtgaagcagg cggatggcac caccaagacg gatgccacca acatcgatgc
     5521 gaccggcaat gtggtgatcg tcaccgcgaa ggagaccatc accggcgatt gggccaagat
     5581 cgacgtgcag agcgacaagc tggtggtggg cggcaacgtg aagctggtgc agggctcgac
     5641 catcctgacc ggtcctgaac tgcgcgccga cctcaagagc aaccggatgg aaatgaaggg
     5701 tggccgcgtc aagggcagct tccttccgaa gtgatgttta aggcaaaatt aaagctattg
     5761 ggtggaatgt ctcttggcgc gtgatgacaa tgtgaaggtg gcgcggaagc cggcgcggac
     5821 tcggaagaag gttcccgagt tccacgacgg aaacgaaggc ctggtggttg agggtctgag
     5881 caagacctac aagcgccggc cggtcgtccg caacgtatcc ctgtcactca ggcgcggtga
     5941 ggccgtggga ttgcttggac ccaatggcgc cggcaagacg accgtgttct acatgatcac
     6001 gggcctgatc gccgccgaca gcggatccat ccgactcgat ggggccgata tcacccacct
     6061 gcccatgtat cggagggcaa ggctgggcat cggatatctg ccgcaggaga gctcgatctt
     6121 ccgcgggctc acggtcgaag aaaacattcg cgccgttctc gaactgacgg aaagttccag
     6181 atcccggcgt gacagtatgt tgcgtgagtt gcttgatgag ttctcgatta cccatctccg
     6241 gaattctccg gctgttgcgc tctctggcgg agaacgtcgc cgcgtggaga tcgcccgcgc
     6301 ccttgccgcc agcccccagt tcatgctcct cgacgagccc ttcgccggaa ttgacccgat
     6361 tgcgatcagt gatattcgca agctggtgcg acagctcacc gctcggggaa tcggggtgct
     6421 tgttacggat cacaacgtcc gcgaaaccct tgaactcatc gatacggccc tcatcatcca
     6481 tgagggccag gtcttgaccg tcggcacacc cgacgagatc gtccgcaacc cggacgtcag
     6541 gcgtcattac ctgggcgccg acttcagcat gtaacggcag ggggacgtcg tgccatggcg
     6601 ataggcccca ggatggaaat gcgccagggt caatccctgg tcatgacccc ggccatgcag
     6661 caggccatca agatgctgcc catgtcgaac atcgatctcc aggcctttct cgaaagcgaa
     6721 cttgaacgca atccgctgct cgagctcgat gacggctcgg aggtgcgcga ggcgccggcg
     6781 tcgcatcagg ccgacaccgt tccggaagtc tcggccgatc cgccgcccga ggacggatat
     6841 gccggttacg acggcgaagc cgacggcagg tcgggcggcg ccggcgagcc ggtcatcgca
     6901 ggtccgggcg ggcttggccc acaggattcg aactggtcct cggtccggtc cggcggcgga
     6961 gccggcttcg acggcgacga catggagttc gcctcgacca tctcgcgcga ggagacgctg
     7021 gtcgagcatc tgagccagca actttcgctg gccatatccg atccggccga cctgctgatc
     7081 ggtcagcaca tcatcggaat ggtcaacgag gcgggctacc tcaccggcga cacggcaggc
     7141 attgccgaca tgctcggcac cagcgtggca catgtcgatc gcgtccttgc cgtcatgcag
     7201 cagttcgatc cgcctggagt tttcgcgcgc gacatgcgcg agtgcctcat gcttcagctc
     7261 atcgaccgca accgctacga tccggcgatg gcgaagctcc tcgacaacct gaagcttctc
     7321 ggggagcgca actatgccgc cctgcgcagc atctgcgaag tcgaccggga cgaccttgcc
     7381 gagatgatcg acgagatcca caggctggaa cccaagccgg gacggcagtt cggctcggat
     7441 cccgtccagc cgatcattgc cgatgtcatc gtgcgcggcg ctccggacgg ttcctggatc
     7501 gtggaactca acagcgagac attgccgaag gtgctggtgc acaatagcta tgcggccaga
     7561 gtctcgaaat cgtcgacgcg cgaggaagac aagctcttca tctccaagat gtccgagtac
     7621 caggcgcatg ccaaatggct tgtcgagagc ctggaccacc gggccaacac gattctgaag
     7681 gtggcacgcg agatcgtgcg ccagcaggat gcctttcttg tccacggcgt gcagcagttg
     7741 cggcccctca acctgcgcaa tgtcgccgat gccatcggga tgcacgaatc gaccgtgagc
     7801 cgcgtgacct cgaacaagta catggcgact ccgcgtggca tcttcgagat gaagttcttc
     7861 ttcacgcgcg ccatcgccgc caccgaatcc ggcgatgagg cgcattccgc cgaagcggtt
     7921 cgccatcgta ttcgcgaact ggttgcagcc gagggcgagg ccgtcctgtc cgacgacgat
     7981 ctggtcggga agctcaagca ggaaggcatt gagatcgccc gcagaaccat cgccaaatat
     8041 cgggaatccc tggggattcg atcttccgtc cagcgccgcc gcgagtggcg cgagaagcat
     8101 cgctgagggc gttgacttcg ccagttgcgg cgactaggtt ccgcgccgct tcgcaaggga
     8161 acgggcgcgc cgccttccgg tcccgcaagg acacctgaaa atccgaagca tgtgaaggcg
     8221 gtaccgcaag atgccgcttg ccagttgacg tttggagcga agacgcccat tttcgatcag
     8281 gggcgaaacg aggattttgc cacgaccatg cagatcaaga tcacgggaaa gaacctcgat
     8341 gtgggtagcg cattgaggga ccacgtggaa gcaaagctcc tccagctgaa gtccaaatac
     8401 ttcgaaggca ccgttcacgg acatgtgacc gtcgagaagc agcgcgggaa ttttcatacc
     8461 gactgcaccc tgcatctggc gacgggactg accctgcagg ccaccggcga tgccgccgag
     8521 gcccatccca gtttcgacgc cgcagccaat cacatcgaaa agcagttgcg ccgctacaag
     8581 cgccggctga aggatcatca caagtcgcgg cgcgaaccgg tgcgccatct ggaagcggcc
     8641 agctatgtca tcacgccggc accggaagag gaggacgagg cggccgggct caatcccgtc
     8701 gtggtcgccg aggcggatgc caggctgccc gagctgtcgg taggggaagc ggtgatgcaa
     8761 ctcgatatct cgaacgtgcc tttcgtgctg ttccgcaacg gcgggcacgg ccgtctgaac
     8821 gtggtttacc gccgcaagga cggcaatatc ggctgggtcg atcccaagcc ggcagcttga
     8881 ccaacaccgg cagccgaagg gcggggagtg gcaccatgag tctggatgag atcatcgacg
     8941 accaatcggt actggccaat gtgaaggcgc agggcaagaa gcagctgctg ctcgaactgg
     9001 cccaggccat ggcgtcgcgc gttgcgatcg atcatcgcgt gatcttcgag accctgctga
     9061 cgcgcgagaa gctcggctcg acgggaatcg gacagggaat cgccatcccg cacggcaagg
     9121 ttcccacgct caatcgcgtt tacggcctgt ttgcgcgcct cgccacgcca atcgacttcg
     9181 agtctgtcga tgggcagccc gtcgatctcg tcttcgtcct gctggcgccg gatcatgccg
     9241 gcgcagatca cctgaaggca ctggcgaaga tttcgcgcct gttgcgtgat cccgtcatgg
     9301 tccagaagct gcgcggcacg gaatcggccg agagcctcta tgccatcctc acggagccgg
     9361 ccgcgacggc gtcaagcgcc accgcggcct agcctccttg ccacgtcagt gcacgctcat
     9421 cggcatgagc tcatgctcga aggcgctgcc caccgccgcc tcgcggctgg gcgagatcga
     9481 gaccggcgtc ccgtcggcgt ggaacaggca gaagagcctg gcattggccg ggatcgtcgc
     9541 ctggccgccg agcatcttcc tggcgtcctt gcccttgatc tcgcggatat aggccagcgc
     9601 gcccccgccc agcttggcca ggtcggattc ggacagcgaa agggtcttct tggtatcctg
     9661 cgtgttcatg gttcctagcc tccaatctcg atacggcgca ccacccgccg cggttctggc
     9721 cgttccaggc tgacgatcag caagccgttt tccagccgtg cggcggtcac attcatgccg
     9781 tcggccagca cgaaggtgcg ggcaaactgg cgggcggcga tgccccggtg aagatatgac
     9841 ctgtcctgat cctcgcgctg ccggcccttg atgacgagct ggttctcctc aacggtgacc
     9901 tcaagctcat cccggctgaa accggcaacc gcaagtgtaa tgcgaagccg ctcggcctcg
     9961 ccctcggccc gcggcaggcg ttcgatattg tagggtgggt aggcctcgcc ggaggtcttg
    10021 gacgcccggt cgagcatccg ctcaagctcg tcgaatccga gcaggaatgg cgaagaaaac
    10081 atggaaacgc gcgacatgac gttggccctt cgataagcag ctcctgtgtc ccgcggccct
    10141 ctggaggcac cgctggacgg atgtcatatg tgaaaggggg gcggccattt caagcggcag
    10201 gccgccggcg gcccgcaggc cttgacccga cgataaaaaa tggaatcgca acccgaattg
    10261 cgatgtggtt atatccagcc gaaacgatca gggagactgc aatgaaaaga cgtaatctgc
    10321 ttgccgccgt cgccgccgtt gccatgtcgg cgaccgctgc tttcgcggca gacgacaagc
    10381 ccgccattgt cttcgacatc ggcggcaagt tcgacaagtc cttcaacgag agcatgtaca
    10441 acggctccga gaagttcaag gccgagaccg gcatcgccta tgtcgtgttg gagatcgcca
    10501 acgaggccca gcgcgagcag gccatccgca acttcgccga ccagggttat tcgccgatca
    10561 tcgcggcggg cttcgcccag gccgcggccg tcgagaaggt ggccaaggaa tatcctgaac
    10621 tcaaattcgc catcgttgat atggtggttg acctgccgaa tgtccagtcc atcgtcttca
    10681 aggagcatga aggttcctat ctcgtcggca tgctggccgg catggcgtcc aagtccggca
    10741 aggtcggctt cgtcggcggc atggacattc cgctgatccg caaattcgcc tgcggctacg
    10801 cgcagggcgt gaaggccgcc aaggccgacg ctacgatctt ccagaacatg accggcgaca
    10861 ccggtgctgc ctggaacgat ccggtcaagg gcggcgagat caccaagggc cagatggccc
    10921 ag
//
LOCUS       DBNGVR010000185         3891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3801017, whole genome shotgun sequence.
ACCESSION   DBNGVR010000185 DBNGVR010000000
VERSION     DBNGVR010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3801017"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="AC16A0_04075"
     CDS             <1..1756
                     /locus_tag="AC16A0_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HIR3104538.1"
                     /translation="LSLFGDLALDGESRGVSAASLGLTDRRGECIYAACPHYRRCFIE
                     RSVRAGRNADIVIANHALVLHQAAVDHALGVATTEEEEAAPGGLRRIVFDEGHHLFDA
                     ADSAFSGHLTALETVELRRWLRGPESERRRGRGLAERIGDMVENDQAAEALMQQVLRA
                     ALMLPGPGWTRRVQAGQPEGPMELFLTLVRQQVLARAEQAGGNTMETDCLPLVDGLSE
                     AAGEVAAALIDLKRPMAGLAAALAKKLDAEAEELSTHDRGRIEAVSRSLRRRGELMVG
                     SWIDMLTRLLDQKNPLFIEWFAIEQAFGREHDVGLHSHWVDPTEPLALAVLKQADGIV
                     ITSATLKDRPPDVPDDWTNAEMRTGAVHLPYPVKRASYDSPFDYPRASSVIVVNDVNR
                     EDMDQVAAAYRELFLAAGGGALGLFTAIARLRAVQKRLLRPLAEAGLPLYAQHVDPMD
                     TGTLVDLFRAERDACLLGTDAVRDGVDVPGDSLRLIVLDRVPWGTPTILERARKEAFG
                     GNAYGDMVVRLRLRQAFGRLIRRAGDRGSFVVLDSRLASRFATAFPPGVAIARMGLVE
                     AIDRVRAAARLDKAPPIA"
     gene            1783..2196
                     /locus_tag="AC16A0_04080"
     CDS             1783..2196
                     /locus_tag="AC16A0_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR3104539.1"
                     /translation="MPGTISIEQALIYTMVTMSGVEGRINPIELAEIGQLVKHLPVFR
                     GFDETRLTTVAQEAGEILQEAEGLNAILGLIKQALPRELRETAYALAVEVAASDLAVG
                     REELRFLAILRDTLELDKLVTAALERSAIARYQTA"
     gene            complement(2204..3538)
                     /locus_tag="AC16A0_04085"
     CDS             complement(2204..3538)
                     /locus_tag="AC16A0_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3104540.1"
                     /translation="MSDSHAPHAQPITNRSVLTVAIPIMLSNVSEPMIGVVNTAVIGQ
                     LPDPYYIGAIAVGALIFSFIFWGFGFLRLSTGGLSAQATGAGDSAELVAVLGRSLMIA
                     AVAGVSLILLGPLIRDTAFDLVGGSAEIRAHGETYFNYRIWSAPAALANYCVMGWFIG
                     QSRAKLAFMVQLFLNLTNMALSAFFVLELGMTSDGVGLAALIAELSAVLLALALALGM
                     LRRMGARFSMARILDRDRLWKTMAMNGDVMIRTLCLVFAFTWFTARGARAGDVVVAAN
                     AVLINLFEVSAYLIDGFAYASEALVGQSVGARQRDRFRAAIWLTSVWAMVVGVICSAV
                     VWFAGPALIDLMTVSPEVRAAAREYLPWAAIAPVLGVICFQFDGIFTGAMATRDMRNM
                     MIVSLAVFMLAWWLLEAPFGNHGLWAALNIFFVARALTFASRMPSLERRAFA"
     gene            3583..>3891
                     /locus_tag="AC16A0_04090"
     CDS             3583..>3891
                     /locus_tag="AC16A0_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3104541.1"
                     /translation="MSLPQHIQNWFAARGWSPRPHQLAVLDHAAAGDSVLLISPTGGG
                     KTLAGFLPSLAELSEQRSGGGIHTLYVSPLKALAVDIARNLEAPVREMGLPITIETRT
                     G"
BASE COUNT          673 a         1330 c         1336 g          552 t
ORIGIN      
        1 cctctccctc ttcggcgatc tcgctctgga cggcgaaagc cgcggcgtct cggccgcctc
       61 gcttggcctc accgaccggc gcggcgaatg catctatgcc gcctgcccgc attaccgccg
      121 ctgcttcatc gagcggtcgg tgcgcgccgg gcgcaacgcc gacatcgtca tcgccaatca
      181 cgcgctggtg ctgcaccagg cggccgtcga ccacgcgctg ggcgtggcca ccaccgagga
      241 agaagaggcc gcgcccggcg gcctgcgccg catcgtcttc gacgagggcc atcacctttt
      301 cgatgcggcg gactcggcct tctcgggcca tctcacggca ctggagacgg tcgaactgcg
      361 gcgctggctg cgcggacccg agagcgagcg ccgccgcggc cgcggccttg ccgaacgcat
      421 cggcgacatg gtggaaaacg accaggccgc cgaggcgctg atgcagcagg tgctgcgcgc
      481 cgcactcatg cttccgggcc ccggttggac ccgccgcgtg caggccggtc agccggaggg
      541 gccgatggag ctgttcctga cgctggtgcg ccagcaggtg ctggcccggg ccgagcaggc
      601 cggcggcaac acgatggaga ccgactgcct gcccctggtc gatgggctct cggaggcggc
      661 aggcgaggtg gcggcggccc tcatcgacct gaagcgcccg atggccgggc tggcggcggc
      721 gcttgccaag aagctcgatg ccgaagccga ggagctctcc acccatgacc gcggccgcat
      781 cgaggccgtg tcgcgctcgc tccgccgccg cggcgaactc atggtcggca gctggatcga
      841 catgctgacg cggctgctcg accagaaaaa tccgctcttc atcgaatggt tcgccatcga
      901 gcaggccttc ggccgcgaac atgacgtggg gctgcattcg cactgggtcg atcccaccga
      961 gccgctggcg cttgccgtgc tgaaacaggc cgacggcatc gtcatcacct cggccacgct
     1021 caaggaccgc ccgcccgacg tgcccgacga ctggaccaat gccgagatgc gcaccggtgc
     1081 ggtccatctg ccctacccgg tgaagcgcgc cagctacgac tcgcccttcg actatccgcg
     1141 ggcgagttcg gtgatcgtcg tcaacgacgt gaaccgagag gacatggacc aggtggcagc
     1201 ggcctatcgc gagctgtttc ttgccgcggg cggcggcgcc ctcggcctct tcaccgccat
     1261 cgcgcggctg cgcgcggtgc agaagcgcct gctgcgcccg ctggccgagg cgggcctccc
     1321 gctttacgcg cagcatgtcg atccgatgga caccggcacg cttgtcgacc tcttccgcgc
     1381 cgagcgcgat gcctgcctgc tgggcaccga cgcagtgcgc gacggcgtgg acgtgccggg
     1441 cgactcgctc cgcctcatcg tgctcgaccg cgtgccctgg gggacgccca ccatcctgga
     1501 acgcgcccgc aaggaggcct tcggcggcaa tgcctatggc gacatggtgg tgcggctccg
     1561 gctgcgccag gccttcggcc ggctgatccg ccgggccggc gaccgcggca gcttcgtggt
     1621 gctggactcg cgcctcgcct cgcgcttcgc caccgccttc ccgcccggtg tcgcgatcgc
     1681 gcgcatgggg ctggtggagg cgatcgacag ggttcgggcg gcggcgcggc tggacaaggc
     1741 accgcccatc gcctagttgt cccggcagag acggagaagg tcatgcccgg caccatcagc
     1801 atcgaacagg cgctcatcta caccatggtc acaatgtccg gtgtcgaagg ccgcatcaat
     1861 cccatcgagc tggccgagat cgggcagctg gtgaagcacc tgccggtgtt ccgcggtttc
     1921 gacgaaacgc ggctgaccac cgtggcgcag gaggccggcg aaatcctgca ggaggccgaa
     1981 gggctgaatg caatcctcgg actcatcaag caggccctgc cgcgcgagct gcgcgagacc
     2041 gcctatgcgc tggccgtcga ggtggccgct tccgatctcg ccgtcggcag ggaggagctg
     2101 cgtttcctcg ccatcctgcg cgacacgctg gagctcgaca agctggtcac cgccgccctc
     2161 gaacggtccg ccatcgcgcg ctatcagacg gcctgagccg cgttcacgca aaggcgcgcc
     2221 gttcgagcga cggcatgcgg ctggcgaagg tgagcgcgcg ggccacgaag aagatgttga
     2281 gcgcggccca gagcccgtga ttgccgaaag gcgcttcgag aagccaccag gccagcatga
     2341 agacggccag cgacacgatc atcatgttgc gcatgtcgcg ggttgccatg gcgccggtga
     2401 agataccgtc gaactggaaa cagatgacgc cgagcaccgg cgcgatcgcc gcccagggca
     2461 gatattcgcg cgccgccgca cggacctccg ggctgaccgt catcagatca atgagcgcag
     2521 gaccggcgaa ccagacgacg gcggagcaga tcacgccaac caccatggcc cagaccgagg
     2581 tcagccagat ggcggcgcgg aagcggtcgc gctgccgtgc gccgaccgac tggccgacga
     2641 gggcttccga ggcataggca aaaccatcga tgagataggc cgagacctcg aagagattga
     2701 tcagcaccgc attggccgcg accaccacgt ctccggccct tgcaccacgc gcggtgaacc
     2761 aggtgaaggc gaagacaagg cagagcgtgc ggatcatcac gtcgccgttc atcgccatgg
     2821 tcttccacag ccggtcgcgg tcgaggatgc gggccatgga gaagcgcgcg cccattcggc
     2881 gcagcatgcc aagggccagc gccagggcca gcagcacggc ggacagttcg gcaatcagcg
     2941 ccgcaagccc cacgccgtcg gaggtcatgc caagctccag cacgaagaag gcggagagcg
     3001 ccatgttggt gagattcaga aagagctgca ccatgaaggc gagcttcgcc ctggactggc
     3061 cgatgaacca gcccatcacg cagtagttgg ccagcgccgc gggcgccgac cagatgcggt
     3121 aattgaaata ggtctcgcca tgggcgcgga tctcggcgga gccgcccacc aggtcgaagg
     3181 ccgtgtcgcg gatcagcgga ccgagcagga tgagcgagac gccggcgacg gcggcaatca
     3241 tcagcgaacg ccccagaacg gcgacgagct cggcggagtc cccggcaccg gtcgcctggg
     3301 ccgagaggcc gccggtcgaa agcctcagga atccgaagcc ccagaagatg aaggagaaga
     3361 tcagcgcgcc gaccgcgatg gcgccgatgt agtagggatc gggcaattgc ccgatcaccg
     3421 ccgtgttcac cacgccgatc atgggctccg agacattgga gagcatgatc gggatggcaa
     3481 ccgtcaggac cgagcggttg gtgatcggct gcgcatgcgg ggcgtggctg tcggacatgg
     3541 cgtcagttgc tttgaacggg gggccaagtc aagctgcggg ccatgagtct tccgcaacac
     3601 atccagaact ggttcgccgc gcgcggctgg tcgccgcggc cgcatcagct ggcggtgctg
     3661 gatcatgcgg cggcaggcga cagcgtgctg ctgatctcgc ccaccggtgg gggcaagacg
     3721 ctggcgggct tccttcccag cctcgccgag ctgtctgaac agcgcagcgg cggcggcatc
     3781 cacacgctct atgtctcgcc gctcaaggcg ctggcggtgg acatcgcccg caacctcgag
     3841 gcacccgttc gggagatggg actgcccatc accatcgaga cccgaaccgg c
//
LOCUS       DBNGVR010000186         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1051283, whole genome shotgun sequence.
ACCESSION   DBNGVR010000186 DBNGVR010000000
VERSION     DBNGVR010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1051283"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1023)
                     /locus_tag="AC16A0_04095"
     CDS             complement(7..1023)
                     /locus_tag="AC16A0_04095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104542.1"
                     /translation="MTTAVSFQHVSRHFGSVRAVDDVSLDIAEGEFFAMLGPSGSGKT
                     TCLRLIAGFEQPTSGHIEIFGETAEGVPPYRRNVNTVFQDYALFPHMTVLENVGYGLM
                     VKGVDKGTRDRKAEEALALVALGGFGTRRPSQLSGGQRQRVALARALVNQPKVLLLDE
                     PLGALDLKLREQMQVELKTLQRNLGITFVFVTHDQGEALSMADRVAIFNEGKIVQVGT
                     PEDIYERPQTRFAADFVGSSNVLSPAFSKAAGGPAHWASLRPEKIGVLRGRAKSPGKD
                     HQVNVTVKAVHYQGSSTRLNTVTPGGEQINVTAPSALGRFSEGETLTLHWAPSVLHVM
                     EKEA"
     gene            complement(1133..>1668)
                     /locus_tag="AC16A0_04100"
     CDS             complement(1133..>1668)
                     /locus_tag="AC16A0_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3104543.1"
                     /translation="DPYELTEEQYKAALDLLRAQRNIVGRYWHDAFIQIDDFKNEGVV
                     ASSSWPFQVNLLQADKQPIASTIPVEGATGWADTTMMHADAAHPNCAYMWMEHSINPK
                     LQGDLAAWFGSVPAVPAACKGNALLTDAGCETNGFGNFEKIHFWRTPVAKCETQSAGC
                     VPYYRWVTDMIAVLGGR"
BASE COUNT          294 a          513 c          541 g          320 t
ORIGIN      
        1 cgctgctcac gcctctttct ccatgacatg cagcacggag ggcgcccagt ggagcgtcag
       61 cgtctcgcct tcgctgaagc ggccaagcgc cgacggcgcc gtgacgttga tctgttcgcc
      121 acccggcgtg acggtgttga ggcgcgtgct ggatccttga tagtgcacgg ccttcaccgt
      181 gacattgacc tggtgatcct tgccgggtga cttggcgcgg ccgcgcagga ctccgatttt
      241 ctccggccgc aggctggccc agtgtgccgg accgccagcg gccttgctga aggcaggcga
      301 caggacgttc gatgacccca cgaagtccgc ggcgaagcgg gtttgcgggc gttcatagat
      361 gtcctccggc gtcccgacct gcacgatctt gccctcgttg aagatggcca cgcgatcggc
      421 catcgacagg gcctcgccct gatcgtgggt gacgaagacg aaggtgattc cgagattgcg
      481 ctgcagcgtc ttgagttcta cctgcatctg ttcgcggagc ttgagatcta gcgcgccaag
      541 cggttcgtcg agcagaagga ccttgggctg gttgacaagg gcgcgggcca gggccactcg
      601 ctgccgctgg ccaccggaca gctgagaagg cctgcgagtg ccgaaacccc caagagccac
      661 gagagcgagg gcttcttcgg ccttgcggtc ccgcgtgccc ttgtccactc ccttgaccat
      721 cagcccatag ccgacgttct ccagcaccgt catgtgcggg aagagggcgt agtcctgaaa
      781 cacggtgttc acgttccgcc gatagggcgg cacgccctcc gccgtctcac cgaagatttc
      841 gatatgcccg gaagttggct gctcgaagcc tgcgatcagc cgcaggcagg ttgtctttcc
      901 cgagccggat ggtccgagca tggcaaagaa ttcgccctcg gcaatgtcga gggacacgtc
      961 atcgacggcg cgtacgctgc cgaaatggcg gctcacgtgc tggaagctca cggctgtggt
     1021 catgtctgcg ggcaactttt tctgacgtcc cgcccatgcg aggggagggt tccggacaag
     1081 cacgaaagga tcggcccctc cctggaaagg aggggccgca gtggtgtgag gatcagcgtc
     1141 cgccgagaac ggcaatcatg tcggtgaccc agcgatagta gggcacgcaa cccgcagact
     1201 gtgtctcgca cttggccacc ggggtgcgcc agaagtggat cttctcgaaa ttgccgaaac
     1261 cattggtttc gcaacccgcg tcggtcagca gtgcattgcc cttgcaggct gcggggacag
     1321 ctggcaccga gccgaaccag gccgctagat cgccctgcag tttcggattg atcgaatgct
     1381 ccatccacat ataggcgcag ttgggatgcg ctgcatccgc atgcatcatg gtcgtgtcgg
     1441 cccagccggt cgcaccttcg accggaatgg tcgaggcgat cggctgtttg tcggcctgaa
     1501 gcaggttcac ctggaagggc catgacgagg aggccacaac gccttcgttc ttgaagtcgt
     1561 cgatctggat gaaggcatca tgccagtagc gaccgacgat gttgcgctgg gcgcgcaaca
     1621 ggtcgagcgc ggccttgtac tgctcctccg tcagctcgta ggggtcct
//
LOCUS       DBNGVR010000187         2011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3604426, whole genome shotgun sequence.
ACCESSION   DBNGVR010000187 DBNGVR010000000
VERSION     DBNGVR010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3604426"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="AC16A0_04105"
     CDS             <1..970
                     /locus_tag="AC16A0_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HIR3104544.1"
                     /translation="AMDRLEEEAGLTMKVGFEHEFTVRGLNQIAHPAYSVASGRAISP
                     LAGHVLQVLAKSGIRLEQFQAEYGTDQFEISSMPADPLAAADRVVLTQEAIRDGARLF
                     GLHASFIPKPAPNAAGNGVHIHFSLYRKGQPVTAAQDWLTDVSAPFVQGILDHAESIV
                     PFTCLSANSYLRLRPNSWVGPYVCVGLRNREAMIRLVPRQPDGDGRHPQASLEFRASD
                     GTANVYLALAALIGAGLDGLKRQKAPINVAKNPELLGDQERKRLGLRLLPQSLPEALN
                     AFDAKVASSWLGAELVEAYLACRREDARQFCNMSDEEAATVLQRVY"
     gene            complement(980..1363)
                     /locus_tag="AC16A0_04110"
     CDS             complement(980..1363)
                     /locus_tag="AC16A0_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HIR3104545.1"
                     /translation="MELWPDILIAAFLVIGGFFGLVGSYGLIKLPNPMTRLHAPTKAT
                     TLGVGGVLIASMLSALFDRGVLSLHELLITLFLLLTAPITAHFIAKTHLHQNVRRADL
                     PDTGTGAPWASESNRDIGSGKGVSE"
     gene            complement(1371..1640)
                     /locus_tag="AC16A0_04115"
     CDS             complement(1371..1640)
                     /locus_tag="AC16A0_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HIR3104546.1"
                     /translation="MIAIALYFAIGCYASGLVMNMWTITRSRSLGDRILALDTMVING
                     IALTVLYGILSQTAVYFELSLIIAMVGFVSTVAYARFMLRGNVIE"
     gene            complement(1637..>2011)
                     /locus_tag="AC16A0_04120"
     CDS             complement(1637..>2011)
                     /locus_tag="AC16A0_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HIR3104547.1"
                     /translation="AIPILTSAYWPNRPKLRRPLKFVAYAMVVLWDICVANLVVARLI
                     LFRRNANLRSAWVVIPLDLRSPEAITVLAGTITMTPGTVSSDVSACGRALLVHCLDAP
                     DPDAVRDEIKSRYESRLKEIFE"
BASE COUNT          385 a          622 c          645 g          359 t
ORIGIN      
        1 ggccatggat cgcctcgagg aagaggcggg cctgacgatg aaggtgggct tcgagcacga
       61 gtttaccgtc aggggcctca atcagatcgc tcacccggcc tattcggttg cctcgggccg
      121 cgccatctcg ccgcttgccg ggcacgtgtt gcaggtgctg gcgaaatccg gcatccggct
      181 ggagcagttc caggccgaat acggtaccga ccagttcgag atttcttcca tgccggccga
      241 tccgctcgcc gccgccgacc gcgtggtgct gacccaggaa gcgattcgcg acggtgcccg
      301 actgttcggc ctgcatgcga gcttcatccc gaagcccgcg cccaatgccg ccggtaacgg
      361 cgtgcacatc cacttcagcc tgtaccggaa aggccagccc gtcacggccg cacaggattg
      421 gctgacggat gtttccgcgc ccttcgtgca gggcatactg gaccacgccg agtcgatcgt
      481 gccgttcaca tgcctctccg ccaattccta cctgcggcta cgccccaaca gctgggtcgg
      541 gccctatgtc tgcgtcggcc tccggaaccg ggaagcgatg atccgcctgg tgccccgaca
      601 gcccgatggc gacggccggc atccgcaggc ctcgctggaa ttccgtgcca gcgatggcac
      661 ggccaatgtc tatctggcgc tggccgcact gatcggagca gggctggacg gtctcaagcg
      721 gcagaaggcg ccgatcaatg tggccaagaa tccggagctt ctcggcgacc aggagcgcaa
      781 gcgactggga cttcggctat tgccgcagtc cctgccggag gcgttgaatg cctttgatgc
      841 caaggttgca tcgtcctggc tcggagcgga gctcgtcgag gcctatctcg cctgccgccg
      901 cgaggacgca aggcagttct gcaacatgag cgacgaggaa gccgcaaccg ttctccaacg
      961 tgtctattga cacgacctgc tattccgaca cgcctttgcc cgagccaatg tcccggttag
     1021 attcgcttgc ccatggcgca ccggttccgg tgtccggaag atccgcccgc cgcacgttct
     1081 gatgcagatg cgtcttcgcg atgaagtgcg ccgtgatagg cgccgtaagc aacaggaaca
     1141 gggtgatcag aagctcgtgc agcgatagga cgccgcgatc gaacagcgca ctcagcatgg
     1201 aggcgatcag cacgccgccc acgcccagcg tggtcgcctt ggtgggggcg tgcagccgcg
     1261 tcatgggatt tggcagcttg atcaggccat aggatcccac caggccgaag aatccgccga
     1321 tcacgaggaa cgcggcaatg agaatgtcgg gccagagttc catgtcaggt tcactcgatg
     1381 acgttgccgc gcagcatgaa gcgtgcgtag gcgactgttg atacgaagcc gaccatggcg
     1441 atgatcagcg acagctcgaa atagaccgcc gtttgcgaca gaattccgta cagcacggtc
     1501 agagcaatgc cgttgatgac catggtgtcg agagccagga tgcggtcgcc gaggctcctt
     1561 gatcgggtga tggtccacat attcatcacg aggccggacg cgtagcagcc gatggcgaag
     1621 tacagggcaa tggctatcac tcgaagatct ccttcaaacg gctttcgtat cggctcttga
     1681 tttcgtcccg taccgcatcg gggtcgggcg catcgaggca atggacaaga agggcgcggc
     1741 cgcaggcgga cacgtcgctc gagacggttc cgggcgtcat ggtgatcgtg cccgccagta
     1801 ccgtgatcgc ttcgggcgat ctcagatcca gcggtatgac cacccaggcg ctgcgcagat
     1861 tggcgttgcg tcgaaacagg atcagacggg ccacgacgag attggcgacg cagatgtccc
     1921 acaggacgac catggcatag gcgacgaatt tcagcggccg ccgaagcttc ggccgattgg
     1981 gccagtaggc gctggtcagg atcggtatgg c
//
LOCUS       DBNGVR010000188         2004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2033536, whole genome shotgun sequence.
ACCESSION   DBNGVR010000188 DBNGVR010000000
VERSION     DBNGVR010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2033536"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="AC16A0_04125"
     CDS             complement(<1..535)
                     /locus_tag="AC16A0_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR3104548.1"
                     /translation="MAANPRIAFIGAGSTVFMKNIVGDILQRKATAGATLALMDIDAA
                     RLAESEVVARKLIASLGVPARIETHSDRRRALAKADFVIVAFQIGGYEPCTVTDFEVP
                     KRFGLRQTIADTLGVGGIMRGLRTVPHLWAVCDDMMELCPNAILLQYVNPMAINTWAI
                     AAKYPAVRQVGLCHSVQG"
     gene            697..1119
                     /locus_tag="AC16A0_04130"
     CDS             697..1119
                     /locus_tag="AC16A0_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3104549.1"
                     /translation="MLARTLAIIPMFFLAILPAQSQESGTNAGVSAEADFRMYCAECH
                     GEEGKGDGPKSFGLSAPSPDLTKLSARNGGTFPREKLADVIDGRADIALHGGREMPVW
                     GTWFKMEAAENLGGAEGDEDSIERRVDNLIAYIEELQE"
     gene            1121..>2004
                     /locus_tag="AC16A0_04135"
     CDS             1121..>2004
                     /locus_tag="AC16A0_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR3104550.1"
                     /translation="MTYHTFIPETYHNTIGWHDPVMFIDDGDTVCAETADAHGFDRHG
                     NQPGSRPNPMTGPFFVRGAEPGDVLKVAINRISMTRSSGWTFSTLAANVVDPAVVRRL
                     PAPEKVLWIIDSDGSVARLAEPPPRLADWTVPLDPMIGCFGVAPALGQAWSTATSGPY
                     GGNMDYRGFRSGVEVMFPVFAPGALFFLGDVHAAQGDGEIVGTGIETSAEVEFTVAVI
                     KKKQIGWPRGETPDEIFAIGNARPLDQALQHATPEMLDWLIAEHGHDAVSASHLLGMA
                     VRYDVANVFNPNYSVACR"
BASE COUNT          370 a          658 c          633 g          343 t
ORIGIN      
        1 tgccctgcac cgagtgacac agacccacct gacggaccgc cggatacttg gcggcgatcg
       61 cccaggtgtt gatcgccatc ggattcacgt attgcagcag gatcgcattg ggacagagtt
      121 ccatcatgtc atcgcagacg gcccacaaat gcggcacggt gcgcaggccg cgcattatgc
      181 cgccaacccc cagcgtatcg gcaatggtct gacgcaggcc gaagcgcttc ggcacctcga
      241 agtccgtcac cgtgcagggc tcgtagccgc cgatctggaa ggcgacgatg acgaaatccg
      301 ccttcgccaa tgcgcggcgg cggtcggagt gggtttcgat cctcgccggt acgcccaggc
      361 tggcgatcag cttgcgggcg acgacttccg actcggccag ccgcgcggca tcgatgtcca
      421 tcagcgcaag ggtggcaccg gccgtcgcct tgcgctgcag gatgtcgccg acgatgttct
      481 tcatgaagac ggtggagccc gcgccgatga acgcaatgcg gggattggca gccatgaaac
      541 gatccttccc ttgggagcat cgggacatag acccatcgcg cccagcccgg caatgcgcag
      601 accggacaat tgcttcccgg atggtaaggc aggcgaagtc ctgctagcct tgctgccggg
      661 aagaacgaac gggcgactca tgatctggag cactccatgc ttgcgcgcac cctagccatc
      721 attccgatgt tctttctggc catcctgcct gcacagtccc aggagagcgg caccaacgcc
      781 ggcgtctcgg cagaggccga cttccgcatg tattgcgccg aatgccatgg cgaggagggc
      841 aagggtgacg gccccaagtc cttcggtctc agcgcgccgt cgcccgacct gaccaagctc
      901 tctgcccgca acggcgggac cttcccgcgc gagaagcttg ccgatgtgat tgacggccgc
      961 gccgacatcg cgctgcatgg cggccgcgaa atgcctgtct ggggaacctg gttcaagatg
     1021 gaggcggcgg agaatctcgg aggcgccgag ggcgacgagg acagcatcga gcgccgcgtc
     1081 gataatctca tcgcctacat cgaagagctt caggagtaga atgacctacc acaccttcat
     1141 tccagagact tatcacaaca ccatcggctg gcatgacccg gtgatgttca tcgatgacgg
     1201 cgacacggtc tgcgcggaaa ccgccgatgc acatggtttc gaccggcatg gcaaccagcc
     1261 cggcagcagg cccaacccga tgacggggcc attcttcgtc cgcggcgccg agccgggcga
     1321 cgtgctgaag gtggcgatca atcgcatctc gatgacacgc tcatccggct ggaccttcag
     1381 cacactggcc gccaatgtcg tcgatcccgc ggtggtacgc cgccttcctg cgccggaaaa
     1441 ggtcctgtgg atcatcgaca gcgatggcag cgttgcgcgg ctggcggagc cgccaccgcg
     1501 gctggcagac tggaccgtcc cgctcgaccc gatgatcggc tgcttcggcg tggcacctgc
     1561 cctgggccag gcctggtcga cggccacaag cggtccctat ggcggcaaca tggactaccg
     1621 cggttttcgc tcgggcgtgg aggttatgtt tcccgtcttc gccccgggcg cgctgttctt
     1681 cctcggcgat gtccatgccg cgcagggtga cggcgagatc gtcggcaccg gaatcgagac
     1741 atcggccgaa gtcgagttca ccgtggcggt catcaagaag aagcagatcg gctggccgcg
     1801 cggcgagacg ccagacgaga tcttcgccat cggcaatgcc cggccgctgg accaggccct
     1861 gcagcacgcc acccctgaga tgctggactg gctcatcgcc gagcatggcc atgatgcggt
     1921 gagcgccagc catctcttgg gcatggccgt tcgctacgac gtcgccaatg tcttcaatcc
     1981 gaactactcg gtcgcctgcc gcat
//
LOCUS       DBNGVR010000189         1768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_857113, whole genome shotgun sequence.
ACCESSION   DBNGVR010000189 DBNGVR010000000
VERSION     DBNGVR010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_857113"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /gene="miaB"
                     /locus_tag="AC16A0_04140"
     CDS             complement(<1..1153)
                     /gene="miaB"
                     /locus_tag="AC16A0_04140"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR3104551.1"
                     /translation="MDPFSKKVFVKTYGCQMNVYDSERMRDVLAPLGYAETDRPEEAD
                     LVILNTCHIREKAAEKVYSELGKIRRMKQDRAAAGREMVIAVAGCVAQAEGEEIVRRA
                     RAVDLVFGPQTYHRLPQLLAAREATGRAVVETEFPAEDKFDKLAARRPSGHGVTAFLT
                     VQEGCDKFCSFCVVPYTRGAEFSRSVAAVVSEAQKLAARGVREITLLGQNVNAYHGEG
                     ASGSVNLAGLVAELSRIPGIERLRYTTSHPRDMTDDLIAAHADNPKLMPYLHLPVQAG
                     SDRILKAMNRGHTSADYLRLVERIRAARPDIAMSGDFIVGFPGETEDDFRATLALVSE
                     VGYAFAYSFRYSPRPGTPAADAGDQVPAEVAQDRLHRLQALLHAQQTAFN"
     gene            complement(1166..>1768)
                     /locus_tag="AC16A0_04145"
     CDS             complement(1166..>1768)
                     /locus_tag="AC16A0_04145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3104552.1"
                     /translation="QPPRQGPVLIASNHVSWIDILVLSAIAPLSFIAKREVGRWPFFG
                     SLARLQRTVFIDRDRRHATGNARDEMRDRFRAGDILVLFAEGTTGDGRSILPFKSAFF
                     GAAEYPGVLVQPVTLAYTGHHGLPMDRRTRPSYAWYGDMDLAPHLIEAIATGPVDVTV
                     IFHEALSLSGERTRKHLAAHAEAEVRAGLVSALHHPAKMG"
BASE COUNT          304 a          548 c          620 g          296 t
ORIGIN      
        1 cattgaaggc ggtctgctgg gcatgcagca gcgcctgcag ccgatgcagc cggtcctgcg
       61 ccacctcggc cgggacctgg tcaccggcgt ccgccgccgg cgttccgggc cgcggcgaat
      121 agcggaacga ataggcgaag gcatagccga cttcgctaac cagtgcgagg gtggcccgga
      181 agtcatcttc cgtctcgccg gggaagccca cgatgaaatc gcccgacatg gcgatatcgg
      241 gccgcgccgc gcggatgcgc tcaaccagcc gcagatagtc ggccgaggtg tggccgcggt
      301 tcatggcctt gaggatgcgg tcgctgccgg cctgtaccgg cagatgcaga tagggcatca
      361 gcttggggtt gtcggcatgg gcggcgatga gatcgtcggt catgtcccgg ggatggctgg
      421 tggtgtagcg cagcctctcg atacctggga tgcgtgacag ttcggcgacg agcccggcca
      481 ggttcaccga tcccgatgcg ccctcgccgt gataggcatt gacgttctgg ccaagcagcg
      541 tgatctcgcg cacgccccgg gcggcgagct tctgcgcctc ggaaaccacg gccgcaacgg
      601 agcgcgagaa ttcggcgccg cgcgtatagg gaaccacgca gaaagagcag aacttgtcgc
      661 agccttcctg cacggtgaga aaggcggtga cgccatgacc ggaaggccgg cgggcggcga
      721 gcttgtcgaa cttgtcctcg gccgggaact cggtttcgac gacagcgcgg ccggtggctt
      781 cgcgcgccgc cagaagctgc gggaggcgat ggtaggtctg ggggccgaag acgaggtcga
      841 cggcgcgagc gcggcgcacg atttcctcgc cttccgcctg ggcgacgcag ccggcaacgg
      901 cgatgaccat ctcccgaccc gcggctgcgc ggtcctgctt catgcggcgg atctttccca
      961 gttcggaata gaccttctcg gccgccttct cgcggatgtg gcaggtgttg aggatgacca
     1021 gatcggcctc ttcgggccga tcggtttcgg catagcccaa gggtgcaagc acatcgcgca
     1081 tgcgctcgct gtcatagacg ttcatctggc agccgtaggt cttgacgaag accttcttgg
     1141 agaaggggtc catgacgggc agctcctaac ccattttggc aggatggtga agcgcgctta
     1201 ccaggccggc gcgcacctcg gcctcggcat gggcggccag atgcttgcgg gtcctttcgc
     1261 ccgagagcga cagggcctcg tggaagatga ccgtgacatc gactggaccg gtggcgatgg
     1321 cctcgatcag gtgcggcgcc aggtccatat cgccgtacca cgcataggac ggccgcgtgc
     1381 gccggtccat cggcagacca tggtggcccg tataggccag cgtcaccggc tggaccagga
     1441 cgcccggata ctcggcggca ccgaagaagg cgctcttgaa gggcaggatg ctgcggccat
     1501 cgcctgtggt gccctcggcg aaaagcacca ggatgtcacc tgccctgaat cgatcccgca
     1561 tctcatcgcg ggcattgccg gtggcatgac ggcggtcgcg gtcgatgaaa acggtacgct
     1621 gcagccttgc caggctaccg aagaagggcc agcggcccac ctcgcgcttg gcgatgaagg
     1681 acagcggtgc gattgcgctg agcaccagga tatcgatcca gcttacgtga ttgctggcaa
     1741 tcagcactgg cccttggcgg ggcggctg
//
LOCUS       DBNGVR010000190         9507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4193606, whole genome shotgun sequence.
ACCESSION   DBNGVR010000190 DBNGVR010000000
VERSION     DBNGVR010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4193606"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4444)
                     /locus_tag="AC16A0_04150"
     CDS             complement(<1..4444)
                     /locus_tag="AC16A0_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0042157 - lipoprotein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein A-IV repeat region-like
                     domain-containing protein"
                     /protein_id="HIR3104553.1"
                     /translation="MASNTTETRRQDKRAAEAEIQRREPPRGSLDPAPVRGRPANEND
                     PGTSMMLARLRRQPSFAPYYAAFALSLAWVAGWFFIFSSTLFGSQGAAAPEGMADTMT
                     ALALLVLPIGLIWVLAYFLWRASQLRQVSEVLMQSAMRLIRPQDIATEGLTTIAQAVR
                     SEVDLLVGGVEHAVQRASVLEEIVHKEIAAIERAFGGNEERIRSLVTGIENQRAALHQ
                     AGLVINGDANPLLARLETNTKNLDHVVANAHDTLARLETGLRDTASGLARVIDDVAGR
                     AHEAGDEISAQTTQMERMSGLMISELRDFSRQLASQVEQLSHAANTLNAETSEFGHTV
                     TGMEASMAQLIRQSIEQLTDINSDITRTIERVSVGSADQMKQTVADLAEVVQSTANNL
                     TYHIKTATGEVAATVERAGIDTTQQIEQSRTLVTQGLQAVAGDYLDRVAQARAELVGY
                     LDNSAESLSDAINQATDQVAGRINAAGSQMIAGLDQTANTLLTELGSMSTGLSGRVEE
                     TTGKLFGEISMRAEGIITRLDDQAGAVFSRLDEQAGLVTHRVEDTANRILSSIEERTA
                     GATLRIGDTASEVLTALEQQNSDSAARITDSAARLQMAISDAAEMLANRLGSTSIDLV
                     GNIDGSFSAALEKLDSTARDFRTLISQTTDSLEDRISGSGDSLNSVMNAAVASASNRI
                     EEVSNLFHTLLEQTTTGVEERVGGSADALRLHVTEAVTAATGKLDETTRLFQQLIGGT
                     SEELEQRIDGAAERLYGHMNATVTSVSDKIDGVSIMVSSRLEQTSAGVTGQLQSVAGS
                     LNEVLVTTSGTIAAHLKETAEIVNREMQESGIALAANIEKSGGLVTERLITVSDSFNH
                     NMATARDDLIGLLNTSAGDITSRLEETAVQLYSRLDTSTSSMTGQIEQSASKLYHRLD
                     QVTLQVSGELEQTAERINERISSTADSVTTQVARTTDVLTSQLDQSSRQLDDLLGATA
                     DRLGGQLQQATHDLNALFTTSSEMLTDQLGQTASTLGDTFSTNSQQLVRTLDETSTEL
                     SSLFNAKTRGLSDQLSQTTTELSALFSANTKMMSEQLDTTANAMTNNFAETAVRVTSQ
                     VNEANTLMVQRLERTSNEVTSQLDSAGSTIFAKIDNTSRELGQRFEIATHLLERVSGE
                     LSGKLEGTGARFAEILDSASSQIITDLSRVRDAFSQGLGQTTLQITGRLEQDSGLMVD
                     RIDRATRELEQASVTTSVKLDDAHRKFSKHVETANTYLADQLSAAASGLDERLETISM
                     HLTGKLEMTGARVSERLDDVTQLVEKSIDKLNDEMERVLVNRKSALDGLIMDASKRAT
                     EIDSVMTSYMNLIEESLASSEARAKDISRIVAEQAAIANRNLEDEIRKLESTSGGQIS
                     QAARVLRDQHERAMTAMNEMLSSTATDFQQTAQDMRITAQQVVKDIDQARAELKRAIL
                     DLPEETRSNADAMRKVVADQISALSALADVVKRQTGVMDISGPGI"
     gene            4773..5147
                     /locus_tag="AC16A0_04155"
     CDS             4773..5147
                     /locus_tag="AC16A0_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR3104554.1"
                     /translation="MAGRSRHNLPLQAEGQAEVFDRGLLARYTLDSPELEREIVDLFL
                     LQLPETVAMMTQEADAGQWRFAAHALKGSASAVGALQLVALARELETAAPDPVSRNLV
                     LADLQLAITQFRAAVARAYSPR"
     gene            5219..5539
                     /locus_tag="AC16A0_04160"
     CDS             5219..5539
                     /locus_tag="AC16A0_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3104555.1"
                     /translation="MAKITFIQHNGVEQTCDGSPGMSVMETAIKNMVPGIDADCGGAC
                     ACATCHVYVEPEWVEKVGPRNPMEEDMLDFAFDVQDSSRLSCQIKVTDALDGLRVRVP
                     AKQF"
     gene            5548..6576
                     /locus_tag="AC16A0_04165"
     CDS             5548..6576
                     /locus_tag="AC16A0_04165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3104556.1"
                     /translation="MSDVITTDAVIIGAGPCGLFAVFELGLVDVKCHVVDILDRPGGQ
                     CAELYPEKPIYDIPALPIVTGAELTSRLLEQIKPFGAEFHYNQMVTELIKHGDNDFEL
                     KTDGGLVFRSKVVVIAAGGGSFQPKKPPIPGIDSYEGTSVFYSVKKMEAFRGKDVLIV
                     GGGDSALDWTINLQPIAKSMTLLHRRDDFRAAPDSVKKMRELVAEGKMQFRLGQVTGL
                     KGEGGVLSAATVKGNDGAVFDIATNAMLPFFGLTMKLGPVAEWGLNLHENLVPVDTAK
                     FETSTPGVFAIGDINTYPGKLKLILSGFHEAALMAQQAFKYKNPGERLLFQYTTSSSS
                     LQKKLGVN"
     gene            6584..7090
                     /locus_tag="AC16A0_04170"
     CDS             6584..7090
                     /locus_tag="AC16A0_04170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3104557.1"
                     /translation="MNGLLAPPFEGELFRSLLALNNAHAEELSYKTAGDLLALFQAAS
                     HVRAEAAGLALFVAFDDGCSYDNPNFAWFRSRYPRFYYIDRVVVAAAARGRGLARRLY
                     GDLERRAQAEGRERLVCEINASPANPQSDSFHRALGFLPVGERSLADRGKTVRYWAKN
                     FSPGSAGA"
     gene            complement(7065..8861)
                     /locus_tag="AC16A0_04175"
     CDS             complement(7065..8861)
                     /locus_tag="AC16A0_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3104558.1"
                     /translation="MEAGSISHYKEFLIILGVSGLVVPLFLRIGINAVLAYLMVGILL
                     SADVLGQLARIFSPLEALVIHHRESIAQMGELGVVFLLFLIGLELSFERLNTMRRLVF
                     GLGGLQVIITLAAIAAILFAIGFDSATALVAGAALSLSSTAIVVQLLSDAKRLGSQTG
                     RTSFAILLFQDLAVIPLLLLVTILGRKSDGSVLVSILTALAQAGVAIAVIVIVGRYAL
                     RPLLRFVAGAQSSDLFMAATLLIAVGAGAAATLAGLSMSLGAFIAGLLLAETEFRRAI
                     EAIIEPFKGLLLGAFFLLVGLNIDLEGLFENPLRIVAAAAALILLKGLIIYGLARLFK
                     VSNMAATETALLLGPCGEFAFVILGSATAMGIFDPRVNADSLLIVTLTMILIPFLARI
                     GTGIRTRVKDRAIAEQLASVELPDAVPQRVILAGFGRVGHLVGSMLTEHNIPYLAVDV
                     DADLVARERKAGYPVYYGDAANAEFLKRCGLDEAGALAVTMDNPSRVDDVARVARSLR
                     SDLKIIARARDERHAMRLYATGVTEAVPETIESSLQLGEALLVETGVPMGLVIASVHE
                     RRDGFRKLLGRPNRREQVEAARKRLRRQQTQG"
     gene            8934..>9507
                     /locus_tag="AC16A0_04180"
     CDS             8934..>9507
                     /locus_tag="AC16A0_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR3104559.1"
                     /translation="MTGLARRGRDVGLPFPGRPGPFNAITDVPGVEVGTVTLISGEGP
                     LVVGKGPVRTGVTAILPRGKDKAQIPCAAGYYALNGNGEMTGVVWIEECGELQTPVTI
                     TNTHSCGVTRDANIRWLVSRGVGTGQDWGLPVAAETYDGDLNDINGFHVTAEHTIAAL
                     DNARSGPVELGSVGGGTGMITYDFKAGNGSS"
BASE COUNT         1680 a         2886 c         3094 g         1847 t
ORIGIN      
        1 atatgcccgg tccggagata tccatgaccc ccgtctgccg cttcacgaca tccgcaaggg
       61 cactgagcgc ggagatctgg tccgccacga ccttgcgcat ggcatcggca ttgctgcggg
      121 tctcttccgg caggtcgagg atggcgcgct tgagctcggc gcgcgcctga tcgatatcct
      181 tgaccacctg ctgggccgtg atccgcatgt cctgcgccgt ctgctggaag tcggttgccg
      241 tcgacgagag catctcgttc attgccgtca tggcgcgctc gtgctggtcg cgcagaaccc
      301 gcgcggcctg ggagatctgg ccgccagacg ttgactccag cttgcggatc tcgtcttcga
      361 ggttgcgatt ggcgatcgcc gcctgctcgg cgacgatgcg gctgatgtcc ttggcgcggg
      421 cctcagaaga agccagcgac tcctctatga ggttcatgta gctggtcatc accgagtcga
      481 tctcggttgc ccgcttgctg gcgtccatga tcaggccatc cagtgccgac ttgcggttca
      541 ccagcacgcg ctccatctcg tcattgagct tgtcgatcga cttctcgacc agctgcgtca
      601 cgtcgtcgag gcgctcagaa acccgggcac cggtcatttc cagctttccg gtcaggtgca
      661 tcgagatcgt ttcgaggcgc tcgtccaggc cactggcagc ggccgacaac tgatcggcca
      721 gataggtatt ggccgtttcg acgtgcttgg agaacttgcg atgcgcatcg tcgagcttga
      781 ccgacgtggt gaccgaagcc tgttccagtt cccgcgtggc gcggtcgatg cggtcaacca
      841 tcaatcccga atcctgttcg aggcgtccgg tgatctgcag tgtcgtctgg cccaggccct
      901 gcgagaacgc atcgcggacg cggctcaggt cggtaatgat ctggcttgag gcgctgtcga
      961 ggatctcggc aaagcgggcc cccgtgcctt ccagcttgcc ggacaactcg cccgaaacgc
     1021 gctccagcag atgagtggcg atctcgaagc gctggcccag ttcgcgcgag gtgttgtcga
     1081 tcttggcgaa gatcgtgctg cccgccgaat cgagctggga cgttacctcg ttggaggtgc
     1141 gctcgagtcg ctgaaccatc agcgtgttgg cctcgttgac ttggctggtc acacgcaccg
     1201 ccgtttccgc gaagttgttg gtcatcgcgt tggccgtggt gtcgagctgt tcgctcatca
     1261 tcttggtgtt ggccgagaag agcgccgaga gctctgtggt ggtctggctg agctgatcgc
     1321 tcaggccgcg cgtcttggcg ttgaacaggc tcgaaagctc ggtcgaggtc tcgtcgagcg
     1381 tcctgaccag ctgctgggaa ttggtcgaga aggtgtcgcc cagcgtgctg gccgtctgtc
     1441 caagctggtc ggtcagcatc tcgctgctcg tcgtgaagag cgcattgagg tcgtgcgtgg
     1501 cctgctgcag ctggcctccg aggcgatccg ccgtggcgcc gagcaggtca tcgagctgac
     1561 gcgatgactg gtcgagctgg ctggtcagaa cgtccgtcgt gcgcgcaacc tgcgtggtca
     1621 cgctgtcagc cgtcgaactg atgcgctcgt tgatgcgttc ggctgtctgt tccagttcgc
     1681 cgctgacctg gagcgttacc tggtcgaggc ggtgatagag cttgctggca gattgctcga
     1741 tctggcctgt catgctgctg gtgcttgtat ccaggcggct atagagctga acggctgttt
     1801 cctcgagccg cgacgtgatg tctccggccg aggtgttgag caagccgatg agatcgtcgc
     1861 gcgccgtggc catgttgtgg ttgaagctgt ccgagaccgt gatgaggcgc tcggtcacga
     1921 gaccgccgga cttctcgatg ttggccgcca gcgcaatgcc ggactcctgc atctcccggt
     1981 tgacgatctc cgccgtttcc ttgaggtggg ccgcaatggt gccgctggtc gtgaccagca
     2041 cctcgttgag ggatccggca accgactgaa gctgcccggt gacgccggcc gaggtctgct
     2101 cgagccggct cgagaccatg atcgacacgc cgtcgatctt gtccgagacg gaggtgacgg
     2161 ttgcgttcat gtgtccatag agacgctcgg ctgcgccgtc gatgcgctgc tcgagttctt
     2221 ccgatgttcc gccgatcagc tgctggaaca gccgcgtggt ttcgtcgagt ttgcctgtgg
     2281 ccgcagtgac cgcttcggtc acgtgaaggc gcagggcatc cgcgctgccg ccaacgcgtt
     2341 cttcgacccc ggttgtcgtc tgctccagca atgtgtggaa gaggttggag acttcctcga
     2401 tgcggtttga ggctgaagcc accgcggcat tcatcacgct gttgagggaa tcgcccgatc
     2461 cgctgatgcg atcctccagg ctgtcggtgg tctggctgat gagcgtgcgg aaatcgcgcg
     2521 ccgtgctgtc cagcttctcg agcgcagcag agaagctgcc gtcgatgttg ccgacgaggt
     2581 cgatcgacgt gctgcccagc ctgttggcca gcatttcggc agcgtcgctg atcgccatct
     2641 gcagacgggc ggctgaatcg gttatgcgcg cggcgctgtc gctgttctgc tgctcaagcg
     2701 cggtgagcac ttcggaagcc gtgtcgccga tgcgcaaggt ggcgccggcg gtgcgttcct
     2761 cgatcgaact cagaatgcgg ttcgccgtgt cctccacgcg gtgcgtgacg agtccggcct
     2821 gctcgtccag gcggctgaag accgcgccag cctgatcgtc gagacgggtg atgatgcctt
     2881 cggcacgcat gctgatctcg ccgaacagct tgccggtggt ttcctcgaca cgtccggaaa
     2941 gcccggtgct catcgagccg agttcggtga gaagcgtatt ggcggtctgg tcgaggccgg
     3001 cgatcatctg ggagccggcg gcgttgatcc ggcccgccac ctggtcggtc gcctggttga
     3061 tggcgtcgga caggctttcg gcggaattgt cgaggtaacc gaccagttcg gcacgggcct
     3121 gcgccacgcg gtcgaggtaa tcgccggcca cggcctgcag gccctgcgtc accagcgtcc
     3181 ggctctgctc gatctgctgc gttgtgtcga tgccggcgcg ctcgacggtg gcggcgactt
     3241 ctcccgtcgc cgtcttgata tggtaggtga ggttgtttgc agtgctctgc accacttcgg
     3301 ccagatcggc gaccgtctgc ttcatctggt cggccgagcc gacggagact cgctcgatgg
     3361 tgcgcgtgat gtccgaattg atgtcggtca gctgttcgat gctctggcgg atgagctgcg
     3421 ccatgctggc ttccatgccc gtcaccgtat gaccgaattc gctggtctcg gcattgagcg
     3481 tgtttgcggc atgggacagc tgctcgacct gcgaggccaa ctggcgcgag aaatcgcgca
     3541 gctcgctgat catcaagccg gacatacgct ccatctgcgt cgtctgggcg ctgatttcgt
     3601 ctcccgcctc atgggccctt ccggcgacgt cgtcgatcac ccgggccagt ccgctggcgg
     3661 tgtcccgcag gccggtttcg agacgcgcca gcgtgtcgtg ggcattcgcc acaacgtggt
     3721 cgaggttctt ggtgttggtc tcgagccgcg ccagcagcgg attggcatca ccattgatga
     3781 caagtcctgc ctggtgaagg gcggcgcgct ggttttcgat gccggttacc aggctgcgaa
     3841 tgcgttcctc gttgccgccg aaggcccttt cgatggccgc gatttccttg tggacgattt
     3901 cttcaagcac agaggcacgc tgcaccgcgt gctcgacccc gccgactagc aagtcaactt
     3961 ccgagcgcac agcctgtgca atggtggtaa ggccctcggt ggcaatgtcc tgcggccgga
     4021 tgagccgcat ggcggattgc atcaggactt cggagacctg gcgcagctgc gaggcgcgcc
     4081 ataggaagta agccagaacc cagatgagac cgatgggcag cactagcagg gcgagcgccg
     4141 tcatcgtatc ggccatgccc tccggcgcgg ccgcaccctg gctgccgaac agcgtgctgg
     4201 agaaaatgaa gaaccaaccg gcgacccagg ccagcgacag ggcaaatgcg gcatagtagg
     4261 gcgcgaagga cggctgccgg cgcaggcgcg ccagcatcat cgaggtgccg ggatcattct
     4321 cgttggcagg ccgtccacgg accggtgcgg ggtcgaggct gccccggggc ggttcacgcc
     4381 gctggatttc ggcctcggcg gcgcgcttat cttgacgccg ggtctcggtc gtattgcttg
     4441 ccatgtgtat tttcccgcat ccactcacaa cgtcccgcgg cgtttttccc gccgctttgc
     4501 ggtcattcca tgacggctgc cggaacaggc ccaactgggc cggcaccggc aatggcctcc
     4561 gtcgcggagt ttccgcagtc gttcacaata gcttaaccaa tggccagctt gaaccttttg
     4621 gccgccggtt tggccgaatt atttaaaatt tggttaagat catgcagaca attgaaaaga
     4681 ttcgacaaac gggtggttca cggattgccg ttaaggattg gcactacagg cggttgcaag
     4741 tcggctgccg cacggggaga cttcaacgga aaatggcagg gcgatcccgg cacaatcttc
     4801 ctctgcaggc cgaagggcag gcggaggtct tcgatcgcgg acttctggcc cgctatacgc
     4861 tggattcgcc ggagctcgaa cgcgagatcg tggacctctt cctgctgcag ctgcccgaga
     4921 ccgtggcaat gatgacgcag gaggccgacg cgggccaatg gcgcttcgcg gcgcacgcgc
     4981 tcaaggggtc tgcgtcagcg gtcggcgctc tccagctggt ggcgctggcc cgtgaactcg
     5041 aaaccgcggc ccccgatccg gtgtcccgca acctggttct ggctgatctg caactggcca
     5101 tcacgcagtt ccgcgcggcc gtggccagag cctattcgcc gcgctaacag ttgtgtttcg
     5161 caccgtgatc ccctagaagg ccgttcgaca aaacgaacgc ctcaacggat tccgcagcat
     5221 ggccaagatt accttcatcc agcacaatgg tgtcgagcag acctgtgacg gttcgcccgg
     5281 catgtcggtc atggagaccg caatcaagaa catggttccc ggcatcgatg ccgactgcgg
     5341 aggcgcatgt gcctgcgcta cctgtcatgt ctatgtcgag ccggagtggg tggagaaggt
     5401 cggcccgcgc aacccgatgg aagaagacat gctggacttt gctttcgacg tacaggattc
     5461 cagccgcctg tcgtgccaga tcaaggtcac cgatgcgctc gacggcctcc gcgtgcgcgt
     5521 tccagcaaag cagttctgag gtttgtcatg tctgatgtga tcacgaccga cgccgtcatc
     5581 attggcgctg gcccctgcgg cctcttcgcc gtcttcgagc tggggctcgt cgacgtgaaa
     5641 tgccatgtcg tcgacattct cgaccgcccc ggcggccaat gtgccgagct ttatccggaa
     5701 aagccgatct acgacattcc ggcgctcccg atcgtcaccg gcgccgagct gacctcgcgc
     5761 ctgcttgagc agatcaagcc gtttggcgcc gagttccact acaaccagat ggtcaccgaa
     5821 ctcatcaagc atggcgacaa cgacttcgaa ctgaagaccg atggcgggct ggtgttccgc
     5881 tccaaggtcg ttgtgatcgc ggccggcggc ggctcgttcc agccgaagaa gccacccatt
     5941 cccggcatcg attcctacga aggaacgtcg gtcttctatt ccgtgaagaa gatggaagct
     6001 ttccgcggca aggatgtgtt gatcgtcggc ggcggcgatt cggcgctcga ctggaccatc
     6061 aatcttcagc ccatcgcaaa gtccatgacg ctgcttcatc ggcgcgacga ctttcgtgcc
     6121 gcacccgaca gcgtgaagaa gatgcgcgag ctggtggccg aaggaaagat gcagttcagg
     6181 cttggccagg tgaccggact caagggcgaa ggcggcgttc tgtcggcagc aacggtaaag
     6241 ggaaatgacg gcgccgtgtt cgacatcgcc acaaacgcca tgctgccctt cttcgggctc
     6301 acgatgaagc ttggtcctgt tgccgaatgg ggactcaatc tgcatgagaa cctggtgccg
     6361 gtggataccg cgaaattcga gacgtccact cccggcgtct ttgccatcgg cgacatcaac
     6421 acctatcccg gcaagctgaa gctcattctc tccggtttcc acgaggccgc gctcatggcg
     6481 cagcaggcct tcaagtacaa gaatccgggc gaaaggctgc ttttccagta tacgacgtca
     6541 agctcgagcc tgcagaagaa gctgggcgtc aactgaggcc gggatgaacg gcttgctggc
     6601 gcctcccttc gaaggcgagc tgttcaggag cctgctggcc ctgaacaacg cgcatgccga
     6661 agaattgtcc tacaagaccg caggcgatct gctcgccctg ttccaagcgg catctcatgt
     6721 ccgcgccgaa gcggcggggc tcgccttgtt tgtcgccttc gacgacggct gcagctacga
     6781 caatccgaac ttcgcctggt tccggtcgcg atatccgcgc ttctactaca ttgaccgcgt
     6841 ggtcgtggct gcagcggcgc ggggcagggg cctcgcgcgt cggctctatg gcgacctcga
     6901 acggcgcgcg caagcagaag gccgcgaaag gctggtctgc gagatcaatg cgagtccggc
     6961 aaatccgcag tccgactcct ttcatcgcgc gcttggtttc ctgcccgtcg gcgaacggag
     7021 ccttgccgat cgcggcaaga ccgtgcgcta ttgggcgaag aacttcagcc ctgggtctgc
     7081 tggcgcctga gtcgcttgcg ggcggcctca acctgttccc ggcggttggg ccgtccaagc
     7141 agcttccgga aaccgtcgcg ccgctcatgc accgaggcga tcaccagccc catgggcacg
     7201 ccggtctcca ccagaagggc ttcgccgagt tgcaggctgg actcgatggt ctcgggcacg
     7261 gcctccgtca cgccggtcgc gtatagtctc atggcgtggc gttcatcgcg cgcgcgggca
     7321 atgatcttga ggtcggaacg gagggaccgg gcgacgcggg ccacatcgtc gacgcgcgat
     7381 ggattgtcca tcgttacggc aagtgcgccg gcctcatcca ggccgcagcg cttcaggaac
     7441 tcggcattgg ccgcatcccc gtagtagacg gggtaacctg ccttgcgctc gcgcgccaca
     7501 aggtctgcat ccacatcgac ggcgagatac ggaatgttgt gttccgtcag cattgatccg
     7561 accaggtggc cgacgcggcc aaatcctgcg aggatcactc tctggggtac cgcatcagga
     7621 agttccacgc tggccagctg ctcggcgatt gcgcggtctt tgacgcgggt cctgataccg
     7681 gtgccgatgc gggcaagaaa gggaatgaga atcatggtga gcgtcacgat cagcagcgag
     7741 tcggcattca cgcggggatc gaagataccc atggccgtgg cgctgccgag gatgacgaag
     7801 gcaaattcac cgcaaggccc cagcagcagt gccgtctcgg tggcagccat gttgctgacc
     7861 ttgaacaacc tcgcgaggcc gtaaatgatg agacccttga gcaggatgag cgctgctgcc
     7921 gcggcgacga ttcgcagcgg attttcgaac aatccttcaa gatcgatgtt gaggccgacc
     7981 agcaggaaga aggcgccgag caggagcccc ttgaagggtt cgatgatcgc ttcgatggca
     8041 cggcggaact ccgtctcggc cagcagcaat ccggcaatga aggcgccaag cgacatcgaa
     8101 aggccggcca gcgtggcggc cgcgccggcg ccgacagcga tcagcagcgt cgccgccatg
     8161 aagagatcgg agctttgggc tcccgcgacg aaacggagca gggggcgcaa ggcatagcgt
     8221 ccgacgatca cgatgacggc aatcgcaaca ccggcctggg ccagggccgt gagaatgctg
     8281 accagcaccg acccatcgga cttgcggcca agaatggtga cgagcaggag cagcgggatg
     8341 acggccaggt cctggaacag caggatggcg aagctcgtgc ggcccgtctg cgaaccgagg
     8401 cgcttggcat ctgagaggag ctgcacgaca attgccgtcg acgacagcga aagggctgca
     8461 ccggcgacga gggccgtggc actgtcgaag ccgatggcga agaggatcgc ggcgatcgca
     8521 gccaaggtta tgatgacctg caatccgccc agtccaaaga ccaggcggcg catggtgttc
     8581 aaccgttcga aggacagttc gagcccgatg agaaagagga ggaagaccac gcccagttcg
     8641 cccatctggg cgatgctctc ccggtgatgg atgacgaggg cctcgagcgg cgagaagatc
     8701 cgggcaagct ggcccagcac gtcggcgctc aagagtatgc cgaccatcag gtaggccagc
     8761 acggcattga tgccgattcg caggaacagg ggcaccacca gcccggaaac ccccaggatg
     8821 atgaggaact ccttgtaatg tgagatggat ccggcttcca tagaggcact cccgtcaggc
     8881 atcatgtggg ataggcaggg aattgcaagg atgtatgtgg agcacttgac agcatgaccg
     8941 ggctagccag acgcggccgc gatgtgggcc tcccctttcc gggacggccc ggccccttca
     9001 atgcgatcac cgatgtaccg ggagtcgagg tcggaaccgt cacgctgatc tcgggcgaag
     9061 gaccgctggt cgttggcaag gggccggttc gcaccggagt taccgccatc cttccgcgcg
     9121 gcaaggacaa ggcgcagatt ccctgtgccg ccgggtatta tgccctgaac ggcaatggcg
     9181 aaatgactgg tgtggtctgg atcgaggagt gcggtgaact gcagacaccc gtcaccatca
     9241 ccaacacgca ttcctgcggc gtcacccgcg atgcaaacat cagatggctg gtgtcgcggg
     9301 gcgtgggcac cgggcaggat tggggactgc ccgtcgcggc cgagacctat gacggcgacc
     9361 tgaacgacat caatggattc catgtcacgg ccgaacacac gatcgcggcg ttggacaatg
     9421 cgcgcagcgg cccggtcgaa ttgggcagtg tgggcggcgg aacgggaatg atcacctatg
     9481 acttcaaggc aggtaacggc tcatcct
//
LOCUS       DBNGVR010000191         3355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_265968, whole genome shotgun sequence.
ACCESSION   DBNGVR010000191 DBNGVR010000000
VERSION     DBNGVR010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_265968"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="AC16A0_04185"
     CDS             complement(<1..364)
                     /locus_tag="AC16A0_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HIR3104560.1"
                     /translation="MDQKPAIPQDAIAQEIWDMKYRLKAADGTPIERTVDETWWRVAR
                     AIAMAEAPAQREHWAGAFHDAMSGFRFLPAGRILAGAGTGRSVTLFNCFVMGTLPDSL
                     SGIFDGLKEAALTMQQGGG"
     gene            complement(410..1138)
                     /locus_tag="AC16A0_04190"
     CDS             complement(410..1138)
                     /locus_tag="AC16A0_04190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3104561.1"
                     /translation="MTVIRSLLFNVLFYVNLVLFLVLGSPFFFTPRIWSIRALQVWAR
                     SSLWLLRVVAGIGMELRGREHIPHGPALIAGKHQSFWETFAILPWLDDPAMVLKKELV
                     YIPWFGWFARKFRMIAVERSAGSAALRNLVRQAEACIAAGRQVVIMPEGTRRAPDDPP
                     AYKPGAAALYATLGVPCVPFALNSGLFWPRRKFLRHPGTIVLEFLPPIPPGLPRKAFQ
                     AALEGAIEPATQALVAEGRAMYKQ"
     gene            complement(1135..2043)
                     /locus_tag="AC16A0_04195"
     CDS             complement(1135..2043)
                     /locus_tag="AC16A0_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR3104562.1"
                     /translation="MTSTSSNRPGAIIPANIGALRSLTVAMTVMCYLACLAIGALVLT
                     NRAVSSWTSGLSREVTVQVRQTKDADVEAEVAKAQEILQDFPGIAAVTVLDREAGAKL
                     LEPWLGSRNLEDLPIPRLIRVAVDEAAPPDYEALGAALEIVKGANLDTHRRWQSELTR
                     MARALSVLAYTVLTLIAVSSVAIVVFGTRAVLQAHRPIVDLLHLVGARDGYIARQIDG
                     RFLKTGLFAGLLGMGLGLVTFFLLSLTGPSGGGGLADASRSLLFAPPESASLSYALLL
                     AVPVMATLISLVTSRVTLMRMLGDVL"
     gene            complement(2040..2696)
                     /gene="ftsE"
                     /locus_tag="AC16A0_04200"
     CDS             complement(2040..2696)
                     /gene="ftsE"
                     /locus_tag="AC16A0_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR3104563.1"
                     /translation="MVRLENVGLRYGHGPEILKDVNFRMDVGEFAFLTGPSGAGKSSL
                     LRLLFLAQRPSRGAIRLFGEDVAKLNRKSLPALRRRIGVVFQEFRLLDHLTTYDNVAL
                     PLRVAGHGEASYRADVSDLLSWVGLAEQARAYPPVLSGGEKQRAAIARAVIAKPELLV
                     ADEPTGNVDPVLANRLLHLFVELNRLGTTVLIATHDHALVQRARAPVYHLEDGHLRLR
                     "
     gene            2821..>3355
                     /locus_tag="AC16A0_04205"
     CDS             2821..>3355
                     /locus_tag="AC16A0_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3104564.1"
                     /translation="MPATMMISCPSCSTRYDLPAQRLAADGTVIKCASCGYSWLESRA
                     IEITSVNPHQLPAVIEHDYEPDHEVRRLVEANREAQEAFAAQRARRNRRMAGWSALAA
                     CAALPLLVALAMPEQVVAMAPASVTAYQTIGWNVNIYGLDFRKVEMQHMIVDGTRVLA
                     VKGEIANVSDDDRKIPWI"
BASE COUNT          608 a         1084 c         1158 g          505 t
ORIGIN      
        1 tgccgccgcc ctgctgcatg gtgagggccg cttccttgag cccgtcgaag atgcccgaca
       61 gcgaatccgg cagcgtcccc atgacgaagc agttgaagag cgtgaccgag cggccggttc
      121 cggcccccgc caggatgcgg cccgccggca ggaagcggaa cccggacatg gcatcgtgaa
      181 aggccccggc ccaatgttcg cgttgtgcgg gggcttcggc catggcgatg gctcgggcca
      241 cgcgccacca ggtttcgtcg accgtccttt cgatgggggt gccgtcagcg gccttgagcc
      301 ggtacttcat gtcccagatt tcctgggcaa tggcatcctg cggaatggcg ggcttctggt
      361 ccatggcgtg cgagtttacg cccaagaccc taacgagtcg agaattcgtt cactgtttgt
      421 acatggcgcg gccctcggcc accagggcct gcgtcgcggg ctcgatggcc ccttccaggg
      481 cggcctggaa ggctttgcgc ggcaggcccg gcgggatggg cgggaggaat tccagcacga
      541 tggtgccggg atggcgcagg aacttgcggc gcggccagaa caggccggaa ttgagcgcga
      601 agggcacgca gggcacgccc agggtggcat agagcgcggc ggcgcccggc ttgtaggccg
      661 gcggatcgtc aggcgcgcgg cgcgtgccct cgggcatgat caccacctga cggcccgcgg
      721 cgatgcaggc ctccgcctgc cgcaccagat tgcggagcgc ggccgaaccg gcgctgcgtt
      781 ccaccgcgat catgcggaac ttgcgggcga accagccgaa ccacgggatg tagaccagct
      841 ccttcttgag caccatcgcc ggatcgtcga gccagggcag gatggcgaag gtctcccaga
      901 aggactggtg cttgccggcg atcagcgccg ggccatgcgg aatatgctcg cggccgcgca
      961 gctccatgcc gatgccggcc accacgcgca gcagccagag cgaagacctt gcccagactt
     1021 gcagcgcgcg gatcgaccag atgcgcgggg tgaagaagaa gggcgaaccc agcaccagga
     1081 agagaacgag gttcacgtag aacagcacat tgaacagcag cgagcggatg acggtcacag
     1141 cacgtcaccc agcatgcgca tgagcgtgac gcgcgaggtc accaggctga tcagcgtcgc
     1201 catcaccggc acggcgagca gcagcgcgta actgaggctc gccgactcgg gtggtgcgaa
     1261 gagcaggctg cggctggcgt cggcaagtcc gccgccgccg cttggcccgg tgagcgagag
     1321 aaggaagaag gtgacgagtc caaggcccat gccgagaagc cccgcgaaga gtccggtctt
     1381 gaggaagcgg ccatcgatct gccgcgcgat atagccgtcg cgcgcgccga cgaggtgcag
     1441 cagatcgacg atggggcgat gggcctgcag caccgcgcgg gtgccgaaga cgacgatggc
     1501 gaccgacgac accgcgatca gcgtcagcac ggtataggcg agaaccgaca gcgcgcgggc
     1561 catgcgggta agctccgact gccagcggcg atgggtatcg agattggcgc ccttgacgat
     1621 ctccaaggcc gcgcccagcg cctcgtagtc gggcggcgca gcttcatcga ccgccaccct
     1681 gatcaggcgc gggatgggaa gatcctcgag attgcggctg ccgagccagg gttcgagcag
     1741 cttggcgccg gcctcgcggt cgagcacggt gacggccgcg atgcccggaa agtcctgcag
     1801 gatctcctgc gccttcgcca cttcggcctc gacatcggcg tccttcgtct ggcgcacctg
     1861 gaccgttacc tcgcgcgaca ggccgctggt ccagctgctc accgccctgt tggtgaggac
     1921 cagcgcgcca atggcgaggc aggcgagata gcacatcacc gtcatggcca cggtgagcga
     1981 acgcagggcg ccgatgttgg cgggaatgat ggcgccgggg cggttggagg atgtgctggt
     2041 catcgcagcc tcaggtgtcc gtcctcgaga tgatagaccg gtgcgcgggc gcgctgcacc
     2101 agggcatgat catgggttgc gatcagcacc gtggtgccga ggcgattgag ttcgacgaaa
     2161 agatgaagga ggcgattggc cagcaccgga tcgacatttc cggtcggctc gtcggccacc
     2221 agcagttcgg gtttggcgat gacggcgcgg gcgatggcgg cgcgctgctt ctcaccgccg
     2281 gaaagaaccg gcggataggc acgggcctgc tcggccaggc caacccagct cagcagatcg
     2341 ctgacgtcgg cgcgatagga cgcctcgcca tggcccgcca cgcgcagcgg cagcgccaca
     2401 ttgtcatagg tcgtcaggtg gtcgagcagg cggaactcct ggaagaccac gccgatgcgg
     2461 cggcggagcg caggaaggct cttgcggttg agcttggcca cgtcctcgcc gaagaggcgg
     2521 atcgcgccgc ggctggggcg ctgggccagg aagagaagcc ggagcagcga ggacttgccg
     2581 gcgcccgatg ggccggtgag aaaggcaaac tcccccacat ccatgcggaa gttcacatcc
     2641 ttgaggattt ccggcccgtg accataacgc aggcccacat tctcaagccg taccacctga
     2701 ttgctccctc cgctcagccc cggtttcagc acaaagcccg ttaacgaggc attaaccgta
     2761 attgcagtta agcttcatcc tgaattctca cggggatttc cccgccaggc ccctctgcgg
     2821 atgcccgcca ccatgatgat ctcctgcccg tcctgttcca cccgctacga tctcccggcg
     2881 cagcgccttg ccgccgacgg cacagtgatc aaatgcgcgt cctgcggcta tagctggctg
     2941 gagagccgcg ccatcgagat cacctcggtc aatccccatc agcttccggc cgtgatcgag
     3001 cacgactatg agcccgatca cgaggtgcgg cggctggtgg aagccaatcg cgaggcccag
     3061 gaggccttcg cggcccagcg cgcccggcgc aaccggcgca tggccggatg gtccgcgctg
     3121 gccgcctgtg cggcacttcc cctgctggtt gcgctggcca tgccggagca ggtcgtcgcc
     3181 atggcgccgg ccagcgtgac ggcctatcag accatcggct ggaacgtgaa catctatgga
     3241 ctcgacttcc gcaaggtcga gatgcagcac atgatcgtcg acgggacgcg ggtgctggcc
     3301 gtcaagggcg agatcgccaa tgtctcggac gacgaccgca agatcccctg gatcc
//
LOCUS       DBNGVR010000192         8431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4000467, whole genome shotgun sequence.
ACCESSION   DBNGVR010000192 DBNGVR010000000
VERSION     DBNGVR010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4000467"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC16A0_04210"
     CDS             <1..287
                     /locus_tag="AC16A0_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3104565.1"
                     /translation="IGARQAQAIGLINQAVPANELGEVVASVTMKIASKSMVTVKTGK
                     QAFYRQLDLQLDEAYAYTARVMTENLLTRDAEEGIAAFIAKRPPQWTDVE"
     gene            284..709
                     /locus_tag="AC16A0_04215"
     CDS             284..709
                     /locus_tag="AC16A0_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3104566.1"
                     /translation="MTPRINLVTLGVADVAASRAFYERLGLKASSSGNDNITFFDVNG
                     VVLALFSHAALADDAAMAAGEPPVFRGVTLAWNAESEAAVDAIFAHARRAGAKIVKPP
                     HKVFWGGYSGYFCDPDGHLWEVAYNPFFPIDASGRIQLP"
     gene            706..1239
                     /locus_tag="AC16A0_04220"
     CDS             706..1239
                     /locus_tag="AC16A0_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3104567.1"
                     /translation="MNHDSYAPDYIRGILQSVKTIALVGASANEVRPSYFVMKYLLDK
                     GYDVIPVNPGLAGQTLLGQTVYATLKDIPRPVDMVDIFRNSEAAGPITDEALSLSPRP
                     KVIWMQLSVRNDDAASRAEAEGVQVVMDRCPKMEYGKLSGEWAWVGGNSGVISSRKQS
                     LHESGKLQSLGLGPKTY"
     gene            1310..1861
                     /locus_tag="AC16A0_04225"
     CDS             1310..1861
                     /locus_tag="AC16A0_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR3104568.1"
                     /translation="MNSAAHDRYSTGAIVLHWLTALLVIFMIVFGEELVEGAEHDDAA
                     GTFLPSIHVSVGVTILVLTLLRLAWRLGHAAPPYPATMKAWELTLAKVTHLLFYALLI
                     AVPLTGWLAFADWVREEPAMSAVRVFGLFQLPAAPLIGGEVKELHEIGSNAMMVLVIL
                     HVLAALKHQFVDGGGVFKRMLPH"
     gene            1932..3209
                     /locus_tag="AC16A0_04230"
     CDS             1932..3209
                     /locus_tag="AC16A0_04230"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HIR3104569.1"
                     /translation="MADKTPGFDTLALHAGAAPDPATGARITPIYQTTAFVFQDVKHA
                     ADLFALRAFGNIYTRIMNPTQSVLEAKVAALEGGTAALAVASGHAAQLLVFHTIMQPG
                     DEFVAVRQLYGGSINQFSHAFKSFGWHVVWADAEDLSSIEKALSPKTKAIFIESIANP
                     GGLITDIAGIAKIAQKAGVPLIVDNTLATPYLCKPIEHGANIVLHSLTKFMGGHGNSM
                     GGVIVDAGNFDWSKSGRYPLLSEPNGSYHGLRLHETFGNIAFAIACRVLGLRDLGPAI
                     APMNAFLILTGMETLSLRMQRHCDNALKVAQWLKKQPQVAWVNYAALPDDKHHALFKK
                     YCPRGAGAVFTFGLKGGHEAGVKAVSSVKLFSHLANIGDTRSLIIHPASTTHSQLSKD
                     QLKLAGASPDVIRLSVGIEDIDDIIADLAQAIA"
     gene            complement(3178..4206)
                     /locus_tag="AC16A0_04235"
     CDS             complement(3178..4206)
                     /locus_tag="AC16A0_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3104570.1"
                     /translation="MHFLDARQRAALPAVRFWLYFTAFLIFCMVIVGGATRLTDSGLS
                     ITEWQPILGAIPPLTEADWLAALEKYRQIPEYQLINKGMSLEDFKFIYWWEWAHRFLG
                     RIIGLVFFIPFAVFALTGRLSRGTAWRCGVLFVLGGLQGALGWYMVSSGLVDRVDVSQ
                     YRLSAHLTLATVIFAAILWVAIGLDGKRHAPRHGMEWAALALVALILLQVAAGGFVAG
                     LDAGMGYNTWPLMDGALVPPGLMIMEPGWRNLFENAMTVQFNHRLLAYVITLAAVAYA
                     LATRTQTAALLLAAVLLQVVFGIWTLLLQVPLWLGLVHQGGALIVLAAALWNLHALLR
                     QSPGQDPR"
     gene            4300..4512
                     /locus_tag="AC16A0_04240"
     CDS             4300..4512
                     /locus_tag="AC16A0_04240"
                     /inference="COORDINATES: protein motif:HMM:NF022456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HIR3104571.1"
                     /translation="MRQRTRKAIGTVATVLFLIVYSLIAMGIGGNYVVGSGLIPELLY
                     FMVAGALWLPVVMALIRWMAKPDPVS"
     gene            complement(4505..5560)
                     /locus_tag="AC16A0_04245"
     CDS             complement(4505..5560)
                     /locus_tag="AC16A0_04245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3104572.1"
                     /translation="MPNTAFLKTSLDLMYYSGATALLQGATTGLGAIFMLHHVFPGGG
                     QQEGFVPNRVLEVDPGFLDAVIGMVKSLGYDLVSIDDAVERVIAGEHDRPFAVFTLDD
                     GYRDNLVHARPVFGRHDCPFTVYVTTSMIDGTCELWWRGLEIAIAQSPRVTASLAGGH
                     IDIETRTVDQKWAAWEALYWPVRTLDQHEQRDWIRAFCTQHGVDLDRLCRESAMTWDE
                     LRTLAADPLCTIGAHTVRHFASACLPPGEALAEMVASADRLTRELGRRPRHFAYPYGD
                     ELSAGPRDFDLAREVPFTSAVTTRKGLIYPAHADHLLALPRLSLSGQLQETRYIKSLL
                     TGLPFLLFNRLHRVNVS"
     gene            5577..6827
                     /locus_tag="AC16A0_04250"
     CDS             5577..6827
                     /locus_tag="AC16A0_04250"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3104573.1"
                     /translation="MPASAGIYRKWHEQSVALDEALAGAAPSVLSAEGLTAEIHTALP
                     PLRRTWTSFEQIAHGTFYQSYLWCSAWLETVGRASQVEPVVVILRDGAHRICALLPLQ
                     IRRVGKLRTLEWLAAPSNNYGYALYHPDFVRAAVTWLGANWPALVRSIGRVDAVVLRD
                     NPERMADIANPLTGMFTSRAANRSYVMRLSPDFASLYGRKRDGDTRRTGRKKEKALSR
                     LGTVEFGLPQGREETRAVIETMFAQQQGRLAEHGIHNVFGPAERAFIHRIAELQDESR
                     PALAPYTLKLNGIVLAVYLGGLYKDTFWALISSLAAGPERKYSPGDIALRLSIERCCA
                     AGLSTYDFSAGSSRYKLSWSDDIIQLHDVIEGTTLAGAAYVAWLRGRSAAKRYIKESD
                     TLLRAASGLRRLLRGQTPVRPISD"
     gene            complement(6808..>8431)
                     /locus_tag="AC16A0_04255"
     CDS             complement(6808..>8431)
                     /locus_tag="AC16A0_04255"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:NF025179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HIR3104574.1"
                     /translation="WLSAQIDELRQKLARSEEAVEKFRAEAGLLQGATTTLGTQEISE
                     LNTQITLARAASTEARARADSIRDLLNSKGSVDASSDVLASAAVQRLKEQRSDAARRL
                     AELSATYLPGHPKMVAAQGEVNSVDRQIRSEALKVVTSLEEQARVADAREKSLRESLD
                     RLKSAEASANIDDVKLKALEREASADRALLETMLSRFAEASSRQDQSAQPGMARIIQS
                     ASVPSTPSFPKRGPIVLLMTLAGAALAIGLAFLAELMSAAARLTERLSEQAQPAVAPA
                     PPVYAVSAMPPPAQLVPAAILNAVQPGPAEAPHAAPRVPEVAMMPQVATVEEAADFLL
                     SPAVNSAAAQLAAWSLGQAAAGGGVRLALVAVNCEAIAAATASVAVARHFAASGKRVV
                     IADLSNGGSQIEDICGVIRGPGLAELLMGSVDFTKVIGRDLGSTAHILRHGNDRSATT
                     RQLIAQRAESVVAALAQVYEVVIINLGEAVADLPSLVRVGQHVAIVAPEFGSTGGVGS
                     LLPVLTAGAQSTCHVLVGPPQAARAELSLKSA"
BASE COUNT         1545 a         2775 c         2660 g         1451 t
ORIGIN      
        1 tcatcggcgc gcgccaggcc caggccatcg gcctcatcaa ccaggcggtt ccggccaatg
       61 agctgggcga ggttgtcgcg tccgtcacca tgaagatcgc ctcgaagtcg atggtgacgg
      121 tcaagaccgg caagcaggcc ttctatcgcc agctcgacct ccagcttgac gaggcctatg
      181 cctataccgc gcgcgtcatg accgagaacc tgctgactcg cgacgcggag gaaggtattg
      241 ccgccttcat cgccaagcgc ccgccgcagt ggaccgatgt ggaatgacgc cgcgcatcaa
      301 tcttgtcacc cttggcgtcg cggacgttgc tgcctccagg gctttctacg agcggctggg
      361 attgaaggca tcatcctccg gcaatgacaa catcaccttc ttcgacgtga acggcgtggt
      421 cctggccctg ttcagtcatg cggccctggc tgacgacgca gccatggcag cgggtgagcc
      481 gcccgtgttt cgcggcgtga ctctcgcctg gaatgcggag agcgaggcag cggtcgatgc
      541 catcttcgcc cacgcccgca gggctggcgc gaagatcgtc aagccgccgc acaaggtgtt
      601 ctggggcggt tactccggct atttctgcga tcccgatgga catctctggg aagtcgccta
      661 caaccccttc ttccccattg atgcatccgg acgaatccag ctgccatgaa ccacgacagt
      721 tacgcacccg actacatccg cggcatcctg cagtcggtga agaccatcgc gctggtcggg
      781 gcctcggcga atgaggtgcg cccgtcctac ttcgtgatga agtacctgct cgacaagggc
      841 tacgacgtta tcccggtcaa tccgggcctc gctggacaaa cgcttctcgg ccagaccgtc
      901 tatgccacgc tgaaggacat tccgaggccc gtcgacatgg tcgacatctt ccgtaactcc
      961 gaggctgccg gcccgatcac cgacgaggcc ctgtcgcttt cgccgcgccc aaaggtgatc
     1021 tggatgcagc tgtcggtacg caatgatgat gcggcgtcgc gggcggaggc cgaaggagtc
     1081 caggtcgtca tggaccggtg cccgaagatg gaatacggca agctgtccgg cgagtgggcc
     1141 tgggtcggag gaaactccgg ggtcatctcg tcacgcaagc agagcttgca cgagagcggc
     1201 aagctccaga gcctgggact gggcccgaag acctactgac gcaccggctt gcgagaatcc
     1261 ctgacgtggt tcactgtcag gcggcgagca agttccggga ggctctcagg tgaacagtgc
     1321 cgcgcatgat cgctattcaa ccggtgccat cgtcctgcac tggcttaccg ccctgctggt
     1381 gatcttcatg atcgtctttg gcgaagaact ggtcgagggc gcggaacacg acgacgcagc
     1441 cggaaccttc ctgccgtcca tccatgtcag tgtcggtgtc acgatcctgg tgctgacgct
     1501 cctgcgcctc gcctggcgcc tcggccatgc ggccccgccc taccctgcca ccatgaaggc
     1561 ctgggagctc accctggcca aggtcaccca cctgctgttc tacgccttgc tgatcgccgt
     1621 cccgctcacc ggctggcttg ccttcgccga ctgggtccgc gaggagccgg ccatgtccgc
     1681 cgtccgggtc ttcgggctgt ttcagctgcc cgccgcgccg ctcatcggcg gcgaggtgaa
     1741 ggaactccac gagatcggca gcaacgccat gatggtcctg gtgatcctgc atgtgctggc
     1801 cgccctgaag caccagttcg tcgacggcgg cggcgtgttc aagcgtatgc ttccgcactg
     1861 acatctcggc aattgtcgtt tccggcggcg cgggctaatg tcccgccccg cacaaggaat
     1921 gggagacgac aatggcagac aagacccccg gcttcgacac gctggcgctt cacgcgggcg
     1981 cggctccgga ccccgcaaca ggcgcgcgca tcacgccgat ctaccagacc accgctttcg
     2041 ttttccagga cgtgaagcac gccgccgacc tcttcgcgct ccgcgccttc ggcaacatct
     2101 acacccgcat catgaacccg acgcagtctg tactcgaggc caaggtcgcg gctctcgaag
     2161 gcggcacggc cgcgctggcc gtcgcgtccg gccatgcggc ccagctcctc gtcttccata
     2221 ccatcatgca gccgggtgac gagttcgtgg ccgtgcgcca gctctatggc ggttcgatca
     2281 accagttcag ccacgccttc aagtccttcg gctggcatgt cgtctgggcc gatgccgagg
     2341 atctctcctc catcgagaag gcgctgtcgc caaagaccaa ggccatcttc atcgagtcga
     2401 tcgccaatcc gggcggcctc atcaccgaca tcgcgggcat cgcaaaaatc gcgcagaagg
     2461 cgggcgtgcc gctgatcgtc gacaacacgc tggccacgcc ttatctctgc aagccgatcg
     2521 aacatggcgc caacatcgtg ctgcattcgc tcaccaagtt catgggcggc cacggcaact
     2581 cgatgggcgg cgtgatcgtg gatgccggca acttcgactg gtcgaagagc ggccggtacc
     2641 cgctgctgtc ggagcccaac ggctcatacc atggcctgcg cctgcatgag accttcggca
     2701 acatcgcctt cgccatcgcc tgccgcgtgc tgggcctgcg cgatctgggg cccgccatcg
     2761 cgccgatgaa cgccttcctg atcctcaccg gcatggagac gctgtcgctg cgcatgcaac
     2821 gccactgcga caatgcgctg aaggtggcgc aatggctgaa gaagcagccg caggtggcct
     2881 gggtaaacta tgccgccctg cccgacgaca agcatcacgc gctgttcaag aagtattgtc
     2941 cgcgcggcgc cggcgcggtc ttcaccttcg gcctgaaggg cggccacgag gcgggcgtca
     3001 aggcggtgtc atcggtgaag ctgttctcac acctcgccaa tatcggcgac acgcgctcgc
     3061 tgatcattca cccggcctcg accacccaca gccagctgtc gaaggatcag ctcaagcttg
     3121 caggcgcatc gcccgacgtg atccgcctct cggtcggcat cgaggacatc gacgatatca
     3181 tcgcggatct tgcccaggcg attgcctgag gagagcgtgc aggttccata gggcggcggc
     3241 gagaacgatc agcgccccgc cctggtggac caaccccagc cagagcggca cctgcaagag
     3301 cagggtccag ataccgaaca ccacttgcag aagcacggcc gcgagcaaca gcgcggccgt
     3361 ttgcgtccgt gtggcaagtg cgtaggccac cgccgccagc gtgatcacat aggcgagaag
     3421 ccggtggttg aactgcaccg tcatcgcgtt ctcgaacagg ttgcgccagc ctggctccat
     3481 gatcatcagc cccggcggca cgagggcgcc gtccatgagc ggccaggtgt tgtagcccat
     3541 gccggcatcg aggcccgcaa caaagcctcc ggcagcgacc tgcagcagga tgagtgcaac
     3601 gagcgcaagt gcggcccatt ccatcccgtg ccgtggtgca tggcgcttgc cgtcgaggcc
     3661 gatggccacc cagaggattg cggcgaagat cacggtggcc agggtcaggt gggccgaaag
     3721 ccggtattgg ctgacgtcga cccggtccac caggccggag gacaccatgt accatcccag
     3781 cgcgccctgc agcccgccca gcacgaacag cacgccgcag cgccatgccg ttccgcgcga
     3841 gaggcgtcca gtcagcgcaa agacggcaaa gggaatgaag aagaccagcc cgatgatgcg
     3901 gccaagaaag cggtgcgccc actcccacca atagatgaac ttgaagtctt cgagggacat
     3961 gcccttgttg atcagctgat actcgggaat ctgccggtac ttttcgaggg ccgccagcca
     4021 gtcggcctcg gtcaggggcg gaatggctcc aaggatcggc tgccattcgg tgatcgacag
     4081 gccgctgtcg gtcaggcgcg tggcgccgcc cacgatcacc atgcagaaga tcaggaaggc
     4141 agtgaaatag agccagaacc ggactgcggg gagcgctgcg cgctgacggg cgtcgaggaa
     4201 atgcatggta gaggccaata gacgcgggcc ctgccccctt caagggatga agcgccgcat
     4261 gcccttgcgc cgggcgtggc ggcagactag gtgacgccca tgcgccagcg tacccgcaaa
     4321 gccatcggca ccgtcgccac cgtcctcttc ctgatcgtct attcgctgat cgccatgggg
     4381 atcggcggaa actatgtggt tggcagcgga ctgatccccg agttgctcta tttcatggtt
     4441 gcgggtgcgc tctggctgcc cgtggtcatg gccctcatcc ggtggatggc caagcccgat
     4501 ccggtcagct gacgttcacc cggtgcagcc ggttgaagag caggaagggc aagccggtca
     4561 gcagcgactt gatgtagcgc gtctcctgaa gctgcccgga cagcgacagg cgcggcaggg
     4621 ccaacagatg atcggcatgt gcgggataga tgagaccctt tcgggtggta accgcgctcg
     4681 tgaagggaac ctcgcgcgca agatcgaagt cgcgcggtcc ggcggaaagt tcgtcgccat
     4741 agggataggc gaaatgccgg ggccgcctgc ccaattcgcg cgtcaatcgg tcggcagaag
     4801 caaccatctc cgccaacgcc tcgccgggcg gcaggcaggc actcgcaaaa tgccggaccg
     4861 tatgtgcgcc gatggtgcac aaggggtcgg cggcgagcgt ccgcagctca tcccaggtca
     4921 tcgccgactc ccggcagagc ctgtccagat cgacgccgtg ttgcgtgcag aaggctctga
     4981 tccagtcacg ctgctcgtgc tggtcgagag tccggacggg ccagtacaag gcctcccagg
     5041 cagcccactt ctggtcgacc gtacgtgtct cgatgtcgat atgcccaccg gcaagcgaag
     5101 ccgtgacccg cggcgactgg gcgatggcga tctccagccc tcgccaccac aattcgcagg
     5161 tgccatcgat catgctggtg gttacataga ccgtgaaggg gcagtcatgg cgaccaaaca
     5221 ccggacgggc atggacaaga ttgtcccgat agccgtcatc gagcgtgaag acggcgaaag
     5281 gcctgtcatg ttcgcctgcg atcacgcgtt cgacggcgtc atcgatgctc accaggtcgt
     5341 agcccaggga cttgaccatg ccaatcacgg catccaggaa acccggatcc acttcgagaa
     5401 cgcggttggg aacgaagcct tcctgctgtc cgccaccagg gaacacatgg tggagcatga
     5461 atattgcacc gagacccgta gttgcaccct ggagcagcgc cgttgcccca gaatagtaca
     5521 tcagatcgag gcttgtcttg aggaacgccg tattcggcat cacaaagggt cccggaatgc
     5581 cagcaagtgc gggaatctat cgcaaatggc atgaacaaag cgttgccctt gatgaagcac
     5641 tggcaggtgc ggcaccctcc gtgctgtcgg cggaagggtt aacggccgag atccataccg
     5701 cgctccctcc cctccgcagg acttggacca gcttcgagca gatcgcacat ggcacgttct
     5761 accagtccta tctctggtgc agcgcctggc tagagacggt cggcagggct tcgcaggtcg
     5821 aaccggtcgt ggtgatcctg cgggatggtg cccatcgcat ctgcgccctc cttcccctgc
     5881 agatcaggcg cgtcggaaag ctgcgcactc tcgagtggct tgccgcgccg agcaacaact
     5941 atggctatgc actctaccat ccggacttcg tccgggccgc agttacatgg cttggcgcca
     6001 attggccggc acttgtgcgg tccatcggca gggtcgatgc cgttgtgcta cgcgacaacc
     6061 ctgagcgcat ggcggacatc gccaatccgc tgacaggcat gttcaccagc cgggccgcca
     6121 accgctccta tgttatgagg ctctcgccgg acttcgcgtc actctacggc cggaagcgtg
     6181 atggcgacac caggcggacg ggacgcaaga aagagaaggc cctctcgcgg ctcggaacgg
     6241 tcgagttcgg cctgccgcag ggacgtgagg aaaccagggc cgtcatcgag accatgttcg
     6301 cgcagcagca aggacgcctc gccgagcatg gaatccacaa tgtcttcggc ccggccgaac
     6361 gcgccttcat ccatcgcatt gccgaactcc aggacgaaag ccggcctgct cttgcaccct
     6421 atacactcaa gctgaacgga atcgtgctgg ccgtctacct gggcggcctc tacaaggata
     6481 cgttctgggc gctcatctcg tcgctggctg ccgggccgga gcgcaagtac tccccgggag
     6541 acatcgccct gcggctgagc atcgagcgct gttgtgcggc gggcctgtcg acctacgatt
     6601 tctcggcggg atcgtcgcgc tacaagctga gctggtccga cgacatcatc cagctgcatg
     6661 acgtcatcga gggcaccacg ttggcgggcg ccgcctatgt cgcctggctt cgtggcaggt
     6721 ccgcggccaa gcgttacatc aaggaaagcg ataccctcct gcgcgccgca tccgggcttc
     6781 gccggctgct ccgcggtcag actccggtca ggccgatttc agactgagtt cggcccgtgc
     6841 ggcctggggc gggcctacca gcacgtgaca tgtcgactgg gctccggcgg tcagaaccgg
     6901 cagcagagac ccgacgcctc cagtgctgcc aaattcgggc gcaacgatcg cgacgtgctg
     6961 gcccacgcga accagcgatg gcagatcggc aaccgcctcg ccaagattga tgatcacgac
     7021 ctcgtaaacc tgtgccagcg ccgcaaccac ggattccgcc cgctgcgcaa tcagttgacg
     7081 ggtcgttgcc gaccggtcgt ttccatggcg caggatatgg gccgtcgacc caaggtcgcg
     7141 gccgatcacc ttggtgaagt cgacggatcc catcagcaac tcggcgaggc caggtccgcg
     7201 gatcacgccg cagatgtcct cgatctgcga cccgccattc gagaggtccg cgatgacgac
     7261 acgcttgcct gaggctgcga agtgccgcgc cacggcgacg gacgccgttg ccgcggcgat
     7321 ggcttcgcag ttcaccgcaa ccagggccag ccgcacaccg ccgccggccg cggcctggcc
     7381 caggctccag gccgcaagtt gggcagcagc cgagttgacg gctggcgaca gaagaaaatc
     7441 ggctgcctcc tcgacggtcg ccacctgcgg catcatggcc acttccggaa cacgcggtgc
     7501 cgcatgcggc gcttccgccg gacccggctg gacggcattg aggatcgcgg caggtacaag
     7561 ctgtgcaggc ggcggcatgg cggacacggc gtagacgggg ggtgccggag ccacggctgg
     7621 ctgagcctgt tcggacaggc gttcggtgag gcgtgccgcg gcagacatca gctcggccag
     7681 gaatgcaagg ccgatagcga gcgcggcgcc ggccagggtc atgagaagca cgatgggacc
     7741 gcgcttggga aaggacggcg tcgaaggaac cgaggcgctc tggatgatcc gcgccatgcc
     7801 cggttgagcc gactggtcct gccgcgaact ggcctcggcg aaacggctca gcatggtttc
     7861 gagcaatgcg cggtcggctg atgcctcgcg ttccagcgcc ttgagcttaa cgtcgtcgat
     7921 gttggcgctg gcctcggccg acttcaaccg atcgaggctc tcgcgcagcg acttctcgcg
     7981 cgcatctgcc acccgtgcct gttcctcaag gctcgtgacg accttcagtg cctccgacct
     8041 gatctggcgg tcgaccgaat tgacctcgcc ctgcgccgcc accatcttgg ggtggccggg
     8101 cagataggtc gccgaaagtt cggccaggcg gcgggcggca tcgctgcgct gctctttcag
     8161 ccgttgaacc gcggcggagg ccagcacatc cgaagatgca tccacactgc ccttgctgtt
     8221 cagcaggtcg cggatggaat cggccctggc ccgcgcctcc gtgctggcag cacgcgccag
     8281 cgtgatctgc gtgttgagtt ccgagatttc ctgcgtgcca agcgtggtcg tggccccctg
     8341 caagaggccg gcttcggccc ggaacttctc gacggcctcc tccgatcttg cgagtttctg
     8401 gcgcagttca tcgatctgtg ccgacagcca g
//
LOCUS       DBNGVR010000193         3516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3607222, whole genome shotgun sequence.
ACCESSION   DBNGVR010000193 DBNGVR010000000
VERSION     DBNGVR010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3607222"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="AC16A0_04260"
     CDS             complement(<1..228)
                     /locus_tag="AC16A0_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3104575.1"
                     /translation="MAQSDLPKNLFVVNHPLILHKLTLMRDKSTPSAVFRQLLREISL
                     LLAYEVARDLPMTMQTIETPLAEMEAPILKGK"
     gene            396..887
                     /locus_tag="AC16A0_04265"
     CDS             396..887
                     /locus_tag="AC16A0_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3104576.1"
                     /translation="MTPAHPIPAGTRIGHVHLKVSDIQKALDFYVGVLGFELQQLYGR
                     QAAFISAGGYHHHIGLNTWESEGGSPPPPGTTGLYHVAILYPTRADLADALKRLAAAG
                     IGLDGASDHGVSEALYLHDPDLNGIELYWDKPEDQWPRNADGSLAMVTRRLDLNDLIN
                     TKH"
     gene            897..1496
                     /gene="wrbA"
                     /locus_tag="AC16A0_04270"
     CDS             897..1496
                     /gene="wrbA"
                     /locus_tag="AC16A0_04270"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIR3104577.1"
                     /translation="MTKLLVLYYSAWGHMEQMAYAAAEGAKEAGAKVDVKRVPELVPD
                     EVAKAAHYKLDQKAPIAKPEELADYDAIIFACGTRYGTMAAQMRNFMDQTGGLWAKQA
                     LAGKVGSAMTSSATQHGGQESTILGFIPTMMHHGMVVVGLPYTWAGQSGVDEIRGGSP
                     YGASTITGSDGSRQPAQHELDGARYQGKHVATIAAKLAK"
     gene            1598..2068
                     /locus_tag="AC16A0_04275"
     CDS             1598..2068
                     /locus_tag="AC16A0_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HIR3104578.1"
                     /translation="MPEPPYVNPHPFIELFIGILFLTVNIFVHGAGVRAISQRFSRSW
                     SKTTPTTPHWHVNLIFAAVIGAFALLHLFETLLWASPIYLTGAIASFRDSYYFVLENY
                     TTLGDDTVNLPPAWQLIGPIIAMAGLFTFGWTGSVLVGIMSEFGRWDRNRVNGA"
     gene            complement(2065..>3516)
                     /locus_tag="AC16A0_04280"
     CDS             complement(2065..>3516)
                     /locus_tag="AC16A0_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3104579.1"
                     /translation="HDLPADAGSFGVMEALALAARPGEAGAGNSRLVELVSSSSAAAS
                     ALLPVTAARADARFVPVLVAIARHHPSMKERTLEVLAGLPLDLSRDAAVPNDLVEAEI
                     VSHNAARRVTGYRLAALAGWPADRILQGLSDPDVLVRRAAVDALQGRAESIESLRPLL
                     RSEDARVRLAAIEALGQAEATDILFAYLRDEAFPRIAAYRGWRKSLPSGNASWVQIAR
                     LAMAEADRAAIDEVLATLEALGHDQAVKYIRRFIGAGDERMRARAAEAISAFDERKLV
                     TPLLPLLDEPSGTRRKRLPLASVIAQMKTSSSPWLRRAAALAKPAKPKEPAMSAAEEG
                     LLDRLLFLRKVPIFESCTLDDLYAIHQVMTRADFADGDLIMEDGAQGEQLFVLLEGTA
                     KVGRRTPSGFVEYTTLSPGSTFGEMALFGDGIRTADVIAAGAVACLVLERSHFEDLIR
                     QQPGILMQICRLFGSRLRAANRGSDALRAEVRS"
BASE COUNT          645 a         1168 c         1116 g          587 t
ORIGIN      
        1 cttgcccttg aggatcgggg cctccatttc ggccagcggt gtttcgatgg tctgcatcgt
       61 catcggcaag tcacgcgcca cctcataggc gagcagcagg ctgatctcgc gcagcaattg
      121 ccggaacacc gccgagggcg tggacttgtc gcgcatcagc gtcagcttgt gaaggatcag
      181 cggatggttc accacgaaca ggttcttggg aagatcggac tgggccatgg tggaattcct
      241 tctgtgcggc tccgttttgc ggaagcccgc cgccgatttc aaggcggcac cgccgtcgtc
      301 caccgttttg gcccggaccg ggcgaaaaga gggcttgcgc atgcaccgcg ccgcatctat
      361 atcggtaaca ctttgcgtta cataaaggca cttccatgac gcccgcccat cccattccgg
      421 ccggaacccg catcggtcac gtccatctga aggtatccga catccagaag gccctggact
      481 tctatgtcgg cgtccttggc ttcgagctgc aacagcttta cggccggcag gccgccttca
      541 tctccgccgg cggctatcac catcatatcg gcctcaacac ctgggagagc gagggcggct
      601 caccgccgcc gccgggtacc acgggcctct atcatgtcgc catcctctac ccgacccgtg
      661 ccgatctcgc cgatgcgctg aagcggctcg ccgccgcggg catcgggctg gatggcgcct
      721 ccgaccacgg cgtctcggaa gccctctacc tgcacgatcc ggacctgaac ggaatcgaac
      781 tctactggga caaaccggaa gaccagtggc cgcgtaacgc tgatggaagc ctggccatgg
      841 tgacccggcg cctcgatctc aacgacctga tcaacaccaa gcactgaagg aacacgatga
      901 caaagctact ggtactctac tattccgcct ggggacacat ggagcagatg gcctatgcgg
      961 ctgcggaagg cgccaaggaa gcgggcgcga aggtcgatgt gaagcgggtt ccggaactgg
     1021 ttcccgacga ggttgccaag gccgcccact acaagctcga tcagaaggcg cccatcgcaa
     1081 agcctgaaga gctcgccgac tacgatgcca tcatcttcgc ctgcggcacc cgctatggca
     1141 ccatggcagc ccagatgcgc aacttcatgg accagaccgg tggtctctgg gccaagcagg
     1201 ccctggccgg caaggtcggc tcggccatga catcctccgc cacccagcat ggcggacagg
     1261 aatcaaccat cctcggattc atccccacca tgatgcatca cggcatggtt gtcgtcggcc
     1321 ttccctatac ctgggccggc caatcgggcg tcgacgagat tcgtggcggt tcgccctatg
     1381 gtgcttccac catcaccggc tccgacggca gccgccagcc ggcccagcac gaactggacg
     1441 gcgcgcgcta ccagggcaag catgtggcca ccatcgcggc caagctcgcg aagtaagagt
     1501 tgcggacacg gcagcccttc ggggccgccg tgtccggcgc tttgtggatg cgggcgtgat
     1561 gggctagcct ccggcaaacc tgtcgaaagg acggaccgtg cccgagcccc cctatgtcaa
     1621 tccccatccg ttcatcgagc tgttcatcgg gattctgttc ctcaccgtga acatcttcgt
     1681 gcatggcgcg ggcgtgcgcg ccatcagcca gcgattcagc agaagctggt cgaagaccac
     1741 acccacaacg ccgcactggc acgtcaatct catctttgcc gcagtgatcg gcgcctttgc
     1801 gctgctgcat ctcttcgaga cgctgctctg ggcatcgccg atctacctga ccggcgccat
     1861 cgcgagcttt cgcgacagct actacttcgt cctggagaac tacacgacgc ttggcgatga
     1921 cacggtcaat cttcctccgg cgtggcagct catcggaccc atcatcgcca tggcggggct
     1981 cttcaccttc ggctggaccg gcagcgttct ggtcggcatc atgtcggagt tcggacgctg
     2041 ggatcgcaac cgggtgaatg gcgcctagct gcgaacctcc gcgcgcaatg cgtcgctgcc
     2101 gcggtttgca gcccgcaggc ggctgccgaa caaccggcag atctgcatga ggattcccgg
     2161 ctgctggcgg atcaggtcct cgaaatgcga gcgctcgagc acgaggcacg cgacagcacc
     2221 ggcggcgatc acatcggcgg tgcggatgcc gtcgccgaag agcgccattt cgccgaaggt
     2281 ggagccgggc gacagcgtcg tgtattcaac gaaccccgaa ggcgtcctgc ggccgacctt
     2341 ggcagttccc tccagcagca cgaagagctg ctcaccctgc gcgccatcct ccatgatgag
     2401 atcgccatca gcgaagtcgg cccgcgtcat gacctggtga attgcataga gatcatccag
     2461 cgtgcagctt tcgaagatcg ggaccttgcg caggaacagc aggcggtcaa gcagtccctc
     2521 ttcggcagcg gacatggcgg gctccttcgg cttggcgggc ttggcaaggg ctgcagcacg
     2581 gcgcagccac ggagaggatg atgtcttcat ctgagcgatg acggatgcca gcggcaggcg
     2641 tttgcgccgc gttcccgacg gctcgtcaag cagcggcagc agcggggtca ccagcttgcg
     2701 ctcatcaaag gccgagatgg cctcggccgc tcgcgcacgc atgcgttcgt cgccggcgcc
     2761 gatgaagcgg cggatgtact tgacggcctg atcgtggccc agggcctcga gcgtcgccag
     2821 cacttcatcg atggccgcgc ggtcggcctc ggccatggcg aggcgggcaa tctggaccca
     2881 gctggcgttg cccgatggca gtgacttgcg ccagccacga taggcggcga tgcgcgggaa
     2941 ggcctcgtcc cgcaggtagg cgaaaagaat atcggtggcc tcggcctggc ccagggcctc
     3001 gatggctgca agccgtaccc gcgcatcctc gctgcgcaag agcggacgaa ggctctcgat
     3061 cgactccgcg cggccctgaa gcgcatcgac ggcggcgcgc cggaccagga catccggatc
     3121 ggagagcccc tgaaggatgc gatctgcagg ccagcctgcc agggctgcaa ggcggtagcc
     3181 ggtcacccgc cgggcggcgt tatgggaaac gatttcagcc tcgacaaggt cattcggcac
     3241 ggcggcatcc cgcgacaggt cgagcggcag tccggccagc acctcgaggg tgcgctcctt
     3301 catggacgga tgatgccgcg cgatcgcgac cagcaccggc acgaagcgcg cgtcggcacg
     3361 ggcggccgtg accggcagca gggccgaagc ggcggcggag gatgaagaga ccagttccac
     3421 cagacggctg ttgccggcgc cggcctcgcc ggggcgcgcg gcaagggcca gggcctccat
     3481 cacgccgaag ctaccggcat ccgcgggcaa gtcatg
//
LOCUS       DBNGVR010000194         2758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4195441, whole genome shotgun sequence.
ACCESSION   DBNGVR010000194 DBNGVR010000000
VERSION     DBNGVR010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4195441"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="AC16A0_04285"
     CDS             complement(<1..435)
                     /locus_tag="AC16A0_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3104580.1"
                     /translation="MSESSAKPPGVSFIHLRTHTAYSLSEGALQIKKLAELAKDAGMP
                     AVAITDTGNLFGALEFSEAMADKGIQPIIGCTLKVDLAEPQKDGASRPAHGLRRVPPL
                     ALLVRNEAGYRNLMKLSSRAYLSSPDNAEHHVSWDYLAAHSGG"
     gene            complement(445..1524)
                     /locus_tag="AC16A0_04290"
     CDS             complement(445..1524)
                     /locus_tag="AC16A0_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3104581.1"
                     /translation="MKAVFSALQLGHAPSRFLSKGYLVSYPESPERARVLLEGARQAG
                     AQICAARRHDPAALTGIHDSSYVAFLQSAHADWSKQEGAAPELMPSLREMVPASVMPE
                     HIMARAGRHLMDFSCAITEKTWTAVQASAMTALTSAELVANGEPVSYALCRPPGHHAY
                     AAHAGGFCYLNNTALAAQRLRQVHDRVAVLDIDVHHGNGTQSIFYARPDVLTVSIHAD
                     PVHYYPFYWGGAEETGEGEGRDFNLNLPVTVGSGDDAWMAAMEKALARIAAYRPGALV
                     IALGLDAHESDPLRGGNVTQAGFARLAELAAALSLPTVIVQEGGYLTEHLSDNLAMFL
                     SAFEGAHSIAVEETPGTVPEAAALD"
     gene            complement(1526..2212)
                     /locus_tag="AC16A0_04295"
     CDS             complement(1526..2212)
                     /locus_tag="AC16A0_04295"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104582.1"
                     /translation="MSTPAPALELRDVARSYAEGKGRLEVFRDLNLMVYPGEIVALVG
                     QSGSGKSSLLHIAGLLEAPTSGEVLIAGRNCSELDDMARTRIRRIGVGFVYQFHHLLP
                     EFSAIENVVIPQLIAGATKAAAKARAEELLIRLGLGERLEHRPAELSGGEQQRVAIAR
                     AIANRPLLLLADEPTGNLDPATAARVHNELLRLIREEGLGALVATHNHDLAAGMTRIA
                     RLDQGRIFEA"
     gene            complement(2209..>2758)
                     /locus_tag="AC16A0_04300"
     CDS             complement(2209..>2758)
                     /locus_tag="AC16A0_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020049503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3104583.1"
                     /translation="DIEALILPLREASGEGMAVQTWKQRNLTFFNALAVERNVMFLVL
                     TMIILVAALNIISGLIMLVKDKGRDIAILRTMGATRGAIQRVFFITGAAIGTAGTIGG
                     FIVGLLICLNVERIRQFISWISGVDPFNPELYYLAQLPAKMEVGQTLLIVGMALALSL
                     GATLYPSWRAAKLDPVEALRYE"
BASE COUNT          507 a          893 c          876 g          482 t
ORIGIN      
        1 cccgccgctg tgcgccgcca ggtaatccca actcacgtga tgctcggcat tgtcgggcga
       61 ggagagatag gcccggctcg acagcttcat caggttgcgg tagcccgcct cgttccgcac
      121 cagcagggcc agcggcggca cccggcgcaa cccatgggcc ggccgtgacg cgccgtcttt
      181 ctgcggctcg gcgaggtcga ccttcagtgt gcagccgatg atcggctgga tacccttgtc
      241 cgccatggcc tcggagaact cgagcgcgcc gaagagattg ccggtgtcgg tgatcgcaac
      301 cgcgggcatg cccgcgtcct tggccagctc cgccagtttc ttgatctgca gcgccccttc
      361 cgacagagag taggcggtat gggtgcggag atggatgaag ctcacgccgg gcggcttggc
      421 tgacgattct gacatgaccc cagtctagtc cagcgccgca gcctccggaa ccgtcccggg
      481 agtttcctca acagcgatgc tgtgggcacc ctcgaaggcg ctcaggaaca tggccagatt
      541 gtccgagaga tgctcggtca gatagccccc ctcctgcacg atcaccgtcg gaagcgacaa
      601 tgccgccgcc agctctgcaa gcctggcgaa acccgcctgg gtaacattgc ccccgcgcag
      661 cggatcgctc tcgtgcgcat cgagccccag cgcgataacc agtgcgcccg gacgatacgc
      721 cgcaatccgg gcaagggcct tttccatcgc cgccatccag gcgtcgtcgc cgcttcccac
      781 ggtcaccggc agattgagat tgaagtcccg tccttcgcct tcgcctgtct cttccgcgcc
      841 tccccagtaa aagggatagt agtgcaccgg atcggcatgg atcgagacgg tcagaacgtc
      901 gggacgggca tagaagatcg actgcgttcc attgccgtga tggacatcga tgtcgagcac
      961 tgcgacacgg tcatggacct gcctgagacg ctgggctgcc agcgccgtat tgttcaaata
     1021 gcagaagcct ccggcatggg ccgcataggc atggtgacca ggcggccggc acagggcata
     1081 tgagacgggt tctccgttgg ccaccagctc ggccgacgta agtgccgtca tggcggaggc
     1141 ctgcacggcg gtccatgtct tctccgtgat ggcacaggag aaatccatca agtggcgacc
     1201 ggcccgcgcc atgatgtgtt caggcatgac cgaggccggc accatctcgc gcaggcttgg
     1261 catgagttcc ggggccgcac cttcctgctt gctccagtcg gcatgggctg actgcaggaa
     1321 agcgacatag gaactgtcat gaatgcccgt cagcgccgcg ggatcatgcc gccgcgccgc
     1381 gcaaatctgg gcgccggcct gccgcgcacc ttccagcagc acgcgcgccc gttcagggct
     1441 ttccggatag gagaccaggt atcccttgct caggaaacgc gacggcgcat ggccaagctg
     1501 cagagccgag aaaaccgcct tcatgtcatg cctcgaagat gcgcccctgg tccagccggg
     1561 caatgcgtgt catgccggcg gcgaggtcgt gattatgggt ggccacaagc gcacccagtc
     1621 cttcttcccg gatcagccgc agcagctcat tgtgtacgcg cgccgccgtc gccggatcga
     1681 gatttccggt gggctcgtcg gctagcagga gcagcggtct gtttgcgatg gcccgggcga
     1741 tggcaacgcg ttgctgttcg ccaccggaga gttcggccgg gcggtgctcc aggcgctccc
     1801 caagtccaag ccggatcaga agctcctccg cccgcgcctt ggccgcagcc tttgtcgcgc
     1861 cggcgatgag ctgtggaatg acgacattct cgatcgccga gaactccggc aggagatgat
     1921 ggaactgata gacgaaaccc acgccgatgc gccggatgcg cgtgcgcgcc atgtcatcga
     1981 gttcactgca gttgcgcccc gcgatcaaaa cctcgcccga agtcggcgcc tctagcaggc
     2041 cggcaatatg gagcagcgac gactttccgg atcccgactg gcccaccagc gccacgatct
     2101 cgccggggta aaccatgaga ttgaggtctc tgaagacttc gaggcggccc ttgccctcgg
     2161 cataggaccg cgccacgtcg cggagttcca gggcgggcgc aggcgtgctc attcgtatct
     2221 cagcgcctcg acgggatcga gcttcgccgc gcgccatgag gggtagagcg ttgcgcccag
     2281 cgacagggcc agcgccatgc ccacgatcag cagggtctgg cccacttcca tcttggccgg
     2341 aagctgagcc aggtaataga gttcgggatt gaagggatcg acgccggaaa tccaggagat
     2401 gaactggcgg atccgctcca cgttcaggca gatcagcagt ccaacgatga agccaccgat
     2461 ggtgccggcc gttccgatgg cagcgcccgt gatgaagaag acgcgctgga tggcgccgcg
     2521 tgtggctccc atggtgcgca ggatggcgat atcgcggccc ttgtccttca ccagcatgat
     2581 gaggcccgaa atgatgttca gcgccgcgac caggatgatc attgtcagta ccaggaacat
     2641 cacgttgcgt tccactgcca gcgcattgaa gaacgtgaga ttgcgctgct tccaggtctg
     2701 aacagccatg ccttcgcccg aggcttcgcg caggggcagg atcagcgcct cgatgtca
//
LOCUS       DBNGVR010000195         1797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1839977, whole genome shotgun sequence.
ACCESSION   DBNGVR010000195 DBNGVR010000000
VERSION     DBNGVR010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1839977"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1291)
                     /gene="metK"
                     /locus_tag="AC16A0_04305"
     CDS             complement(98..1291)
                     /gene="metK"
                     /locus_tag="AC16A0_04305"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3104584.1"
                     /translation="MSRKNYLFTSESVSEGHPDKVCDRISDEVVDLFYREALAQGFDP
                     LQVRVACETLATTNRLVIAGETRGPSSVTPEKIEAVARKAIKDIGYEQAGFHWETVKI
                     EILLHSQSADIAQGVDAGEKKDEGAGDQGIMFGYACDETPELMPAPIYYSHRILSLLA
                     EARHSGKEAALGPDAKSQVTVEYKNGKPVAATQIVVSHQHVDESLSSADVLALVKPYV
                     KKALPKGWIRKDTVWHVNPTGKFYIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGK
                     DPTKVDRSAAYAARYLAKNVVAAGLARRCTIQLSYAIGVSQPLSIYVDTHGTGKVSEA
                     KLEKALAKVMDLSPRGIRTHLGLNRPIFARTSAYGHFGRKPEKDGGFSWEKTDLVKKL
                     KAAVK"
     gene            complement(1396..1797)
                     /locus_tag="AC16A0_04310"
     CDS             complement(1396..1797)
                     /locus_tag="AC16A0_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3104585.1"
                     /translation="MTRRDPNFVDVHVGSRIRMRRQFVGMSQEKLGELLGITFQQVQK
                     YEKGSNRISASRLYYTAKILGVPVQFFFEELPGSEDRSSQDGTREQDTVLSSLMNAEG
                     IVLAKAFREADNSNKRKLIASVARLIAEAKD"
BASE COUNT          307 a          566 c          553 g          371 t
ORIGIN      
        1 agcgccggca gcagctcatc gacgcgctgc aaatgatgac ggcgcaagga tttccccttg
       61 cgccgcccgt agaactgata gcgacgttcc ggctcgctca cttcaccgca gctttgagct
      121 tcttcaccag atccgtcttc tcccaggaga agccgccgtc cttctccggc ttgcgtccga
      181 agtgcccata ggcggaggtg cgggcaaaga tcgggcgatt gagtccgaga tgcgtgcgga
      241 tgccgcgcgg ggacagatcc atcaccttgg ccagcgcctt ctcgagcttg gcttccgaca
      301 cctttccggt gccgtgggta tcgacgtaga tcgaaagcgg ctgcgacacg ccgatggcat
      361 aggacagctg aatggtgcag cgccgggcca gtcccgccgc caccacattc ttggcgagat
      421 agcgtgcggc ataggcagcc gaacggtcga cctttgtcgg gtccttgccc gaaaaggcgc
      481 cgccgccatg cggcgcagcg ccgccatagg tatcgacgat gattttgcgg ccggtgagac
      541 cggcatcgcc gtccgggccg ccgatgtaga acttgccggt cggattgacg tgccagacgg
      601 tgtccttcct gatccagccc ttgggcaggg ccttcttcac atagggcttc accaaggcga
      661 ggacatcggc tgaggagagg ctctcgtcca catgctggtg ggacaccacg atctgcgtgg
      721 ccgcgaccgg cttgccgttc ttgtattcga cggtgacctg gcttttcgcg tcgggcccca
      781 gtgcggcttc cttgccggaa tgccgtgcct ccgccagaag gctgaggatg cggtgcgaat
      841 agtagatcgg ggccggcatg agttccggcg tctcgtcgca ggcataaccg aacatgatgc
      901 cctggtcgcc ggcaccttca tccttcttct cgccggcatc gacgccctgc gcgatatcgg
      961 ccgactgcga gtgcagcagg atctcgatct tcaccgtttc ccagtgaaag ccggcctgtt
     1021 cgtagccgat gtccttgatg gccttgcgcg ccaccgcttc gatcttctcg ggcgtgaccg
     1081 atgatgggcc gcgggtctcg ccggcgatca ccaggcgatt ggtggtggcc agcgtttcgc
     1141 aggccacccg cacctgcaag ggatcgaagc cctgagccaa ggcctcccgg tagaacaggt
     1201 cgacgacctc gtcggagatc cggtcgcaga ccttgtccgg atgtccttcg gacacggatt
     1261 cgctcgtgaa gagatagttc ttgcgtgaca tgcgtgattc atccccgcgg aagcccaccg
     1321 ccttttggca gcggcccact ccaaggtcaa gtcaggggcc ttacattaaa tcagacgccc
     1381 ggaccgctgc ggacttcagt ccttggcctc ggcaatgagc cgcgccacgc ttgcgatcag
     1441 tttgcgcttg ttggagttgt cggcttcgcg gaaggccttt gcgaggacga tgccctcggc
     1501 attcatcaac gacgacagga ccgtgtcctg ctcgcgcgtc ccgtcctggc tgctgcggtc
     1561 ttcgctgccc ggaagttcct cgaagaagaa ctgaacggga acgccgagga tcttggcggt
     1621 gtagtagagc cggctcgcgc tgatgcggtt cgagcccttc tcgtatttct gcacctgctg
     1681 aaaggtgatt cccaggagtt cgccaagctt ctcctggctc attccgacaa attgccgacg
     1741 catgcggata cggctgccca catggacatc aacaaagttt ggatcgcgtc ttgtcat
//
LOCUS       DBNGVR010000196         2925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_661377, whole genome shotgun sequence.
ACCESSION   DBNGVR010000196 DBNGVR010000000
VERSION     DBNGVR010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_661377"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..481
                     /locus_tag="AC16A0_04315"
     CDS             191..481
                     /locus_tag="AC16A0_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3104586.1"
                     /translation="MADYILFMHDDAVADDPAAWDTYLRALRQSGAFEGGSAIGDGVC
                     LRNTGAAAPITRHLTGFIRVTAAGIDAARSLLAGNPHFEAGGTVEIRELPRG"
     gene            complement(497..1678)
                     /locus_tag="AC16A0_04320"
     CDS             complement(497..1678)
                     /locus_tag="AC16A0_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3104587.1"
                     /translation="MKIEAVDFFYLSMPVVADAGDGSQDALLVRVAAGGEVGWGECEA
                     SPLTSIAAFVCPMSHGACKPVRDVVLGKALSGPDDIAALAAAIEMECMDLLQAVHTFS
                     GIEMALWDLLGRKRGVPVYQLLGYAKAYPKLPYASQLFGDTPAETMAGCRAARSRGFR
                     AVKCGWGPFGRGSLKDDRDHLQAAREGLGPDGKLLIDAGQIFRENVDAAAARIALLEE
                     VDAGWFEEPFLGGAYAAYGALSQRRGKVKVAGGEASHNFHMAQHLIDYGKVSFIQIDC
                     GRIGGLAPAKRVADYAVAHGVTYVNHTFTSHLALCASLQPYAGLKDHVICEYPFAPKP
                     VAWDMSETHLEPDANGEVRVPDAPGLGIAMSKSGIRKYLVDVEIRAKGMLLYRTPDPL
                     A"
     gene            complement(1690..2859)
                     /locus_tag="AC16A0_04325"
     CDS             complement(1690..2859)
                     /locus_tag="AC16A0_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3104588.1"
                     /translation="MKITKVECFVLLVPDFRTDACSSAQDNLVVMIHTDDGHVGIGET
                     DTNPWVARAMIEAQGTHIMGLGLTEMLMGQDPTDVEGLWEKMYRGSAMTGRRGLGICA
                     IGALDMALWDLRGKIEGKPCWHLMGGATNPTITPYASLLPEGDDLETYGRVLCERALA
                     AQKLGFRAAKLEICLKGPYSHNSLQIADDRECARMVRDIRKAVGPEMTLMVDVAYAWP
                     DWKEALRAIEMFAGEDIYFVETPLPSDDVEGYAKLCHASPVKIALGEWQNTRFEFLEF
                     MNAKAIDVVQPDVGRVGGLTEAKRVAMHARDRGLVVVPHCWKSAIGIAASVHLAAVAP
                     TCAYIEFLPRELADSALRRDLCSVELPVVDGRIPLPTTPGLGVTLNEEALHRFRV"
BASE COUNT          514 a          974 c          933 g          504 t
ORIGIN      
        1 caatgagatc gcgggccttt tcctttttgg gggcggagcg gtttgcaccg gatttgcgcg
       61 acgtcccgga tggccgccgt tacgccgccg gtctttgatg aggggagctg ggttcggcaa
      121 cgctgatcgc cgttccatgc gttggcagat tggcgcagac tcgacgacga agtgatgcgg
      181 ggagcctgcg atggcggact acatcttgtt catgcatgac gatgccgtgg cagacgaccc
      241 cgccgcgtgg gatacttacc ttcgagcgct gaggcaaagc ggcgctttcg agggcggcag
      301 cgcaatcggc gacggagtct gcctgcggaa caccggtgcc gcagcgccga tcacccggca
      361 cttgaccggc ttcatccgtg tgacggccgc cggcatcgac gcggcacgat cactcctggc
      421 cggcaatccg cattttgaag ccggcggcac cgtggagatt cgcgaactgc cgcgaggctg
      481 agacgaagcg aaggagtcat gccagcggat cgggcgtccg gtagagcagc atgcccttcg
      541 ccctgatctc cacgtccacg agatatttcc tgataccgct cttgctcatc gcaatgccga
      601 ggcccggcgc atccggtacg cgcacctcgc catttgcatc tggctccagg tgggtttcgc
      661 tcatgtccca ggccaccggc ttcggcgcga aagggtactc gcagatcacg tgatccttga
      721 ggccggcata gggctggagc gaggcgcaga gcgccagatg ggaggtgaaa gtgtgattca
      781 cataggttac gccatgggcc acggcatagt ccgccacccg ctttgccggt gccaggccgc
      841 cgatgcggcc gcagtcgatc tggatgaagg agaccttgcc gtagtcgatc aggtgctggg
      901 ccatgtggaa gttgtgcgag gcctcgccgc cagcaacctt caccttgccg cgccgctgcg
      961 acaaggcgcc ataggccgca taggcgccgc cgaggaaggg ctcctcgaac catccggcgt
     1021 cgacctcttc gagcagcgcg atgcgtgccg ccgcagcatc cacattctcg cggaagatct
     1081 ggcccgcatc gatgagcagc ttgccgtcgg ggcccaggcc ttcacgcgcc gcctgcaggt
     1141 ggtcgcgatc gtccttgagg ctgccgcggc caaagggacc ccagccgcat ttcaccgccc
     1201 ggaagccgcg cgaccgggcc gcacggcaac cggccatggt ctccgccggc gtgtcgccga
     1261 aaagctgcga agcatagggc agcttcggat aggccttcgc gtaacccaga agctggtaga
     1321 ccggcacgcc acgcttgcgg ccgagcaggt cccacagcgc catttcgatt ccggagaatg
     1381 tatgtaccgc ctgcagcagg tccatgcact ccatctcgat agccgcggcc agggcggcaa
     1441 tatcgtcggg gccgctcaaa gcttttccca gcaccacgtc gcgcaccggc ttgcaggcgc
     1501 catgcgacat gggacagacg aaggcggcga tcgaggtgag cggcgaggcc tcgcattcgc
     1561 cccagcccac ctcgccgcct gcggcaaccc gcaccagcaa ggcgtcctgg ctgccatcgc
     1621 cggcatcggc caccaccggc atggagagat agaaaaagtc tacggcttcg atcttcatca
     1681 gtgtcctcgt caaacgcgaa atctgtggag cgcttcttcg ttcagcgtca cgccgagacc
     1741 tggcgtcgtc ggcaagggga tgcggccatc caccaccggg agttcgacgc tgcacaggtc
     1801 acggcgcagc gccgagtcgg caagctcgcg cggcagaaac tcgatgtagg cgcaggtcgg
     1861 cgcgacggcg gcaagatgca ccgaggcggc aatgccgatg gccgacttcc agcagtgcgg
     1921 caccacgacc agaccccggt cgcgcgcatg catggccacg cgcttggctt cggtgagtcc
     1981 gccgacgcgc ccgacatcgg gctgcaccac gtcgatcgcc tttgcattca tgaactccag
     2041 gaactcgaag cgcgtgttct gccattcgcc cagtgcgatt ttcaccggcg aggcgtgaca
     2101 gagcttggca tagccttcga catcgtcgga gggaagcggc gtctcgacga aatagatgtc
     2161 ctcgcccgcg aacatctcga tggcgcgcag cgcctccttc cagtccggcc aggcataggc
     2221 gacgtcgacc atcagcgtca tctccgggcc gaccgccttg cggatgtcgc gcaccatgcg
     2281 ggcgcattcg cggtcatcgg cgatctgcag cgaattgtgc gagtaaggcc ccttgaggca
     2341 gatttccagc ttggccgccc ggaagcccaa cttctgcgcg gccagcgcgc gctcgcacag
     2401 cacgcggcca taggtctcga gatcgtcacc ctccggcagg agcgaggcgt agggcgtgat
     2461 cgtgggattg gtcgccccgc ccatgagatg ccagcacggc ttgccctcga tcttgccgcg
     2521 cagatcccac agcgccatgt cgagcgcgcc gatggcgcag atgccgaggc cgcgccggcc
     2581 ggtcatggcg gagccgcgat acatcttctc ccacaagccc tcaacgtccg tcggatcctg
     2641 tcccatgagc atctcggtga ggccgaggcc catgatgtgg gtgccctgcg cctcgatcat
     2701 ggcccgcgcg acccagggat tcgtgtctgt ctccccgatt cccacatggc cgtcgtcggt
     2761 atggatcatg accaccagat tgtcctgcgc cgaggaacag gcatcggtgc ggaagtcggg
     2821 caccaacaat acgaaacact cgaccttcgt gatcttcatc gctttgtcct ccgctgcggt
     2881 attgcttcga cgtcgcccgg ccatgtggcc cggcgcttca ggtca
//
LOCUS       DBNGVR010000197         4301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4787518, whole genome shotgun sequence.
ACCESSION   DBNGVR010000197 DBNGVR010000000
VERSION     DBNGVR010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4787518"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..421
                     /gene="hspQ"
                     /locus_tag="AC16A0_04330"
     CDS             83..421
                     /gene="hspQ"
                     /locus_tag="AC16A0_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HIR3104589.1"
                     /translation="MTPLPAARFTIGQVVKHRLFPFRGVIFDVDPVFNNTEDWWLSIP
                     EEIRPRKDQPFYHLLAENDDTEYVAYVSEQNLLIDDTGEPIRHPQVREYFEGFDPKRK
                     SYRPRRRISH"
     gene            485..1678
                     /locus_tag="AC16A0_04335"
     CDS             485..1678
                     /locus_tag="AC16A0_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HIR3104590.1"
                     /translation="MTFVFITVMMDMIGFGLVMPVLPRLIEEVGGTDLAGASLIAGWM
                     YFAYGGMQFLFGPAVGNLSDAYGRRPLLLLSVFGLAVDFLFTALAPTLFLLFVGRVAA
                     GICGASYTIANAYLADITKPEDRARVFGLMGAAFGLGFVIGPAIGGLLGEFGPRVPFF
                     VAAALTGMNFVFGYFVLPETLPPDQRRPFSLRRSNPVGALKVFASYRGVVPLAAVMFA
                     YFLATSVYPAIWAFWGIARFGWSEAMIGLTLAAFGLITAIIQGTLTGPTVKWLGEKNT
                     VIFGLAASVIAAAGYVWAPGLLAVMVLLVIHAPEGFVHPALTAMMSKSAPPDAQGELQ
                     GGIASLQSIAMLIGTVFFAQVFGWFMQPTSPIITPSAGYVIAAVILAGVLAFFLLLRQ
                     QRVDD"
     gene            complement(1675..2808)
                     /gene="alr"
                     /locus_tag="AC16A0_04340"
     CDS             complement(1675..2808)
                     /gene="alr"
                     /locus_tag="AC16A0_04340"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3104591.1"
                     /translation="MSHRDARPVWGEIDLAAITHNVGLLRERAGRPVSILAPVKANAY
                     GHGMAAVSLHLQKLGVEGLATANFDDAVAARRAGVTIPILLYGAQLPSGHVDVLDQGL
                     TPSVYDRESLQVLAQLGSRRANPVPVHIKVDAGLGRLGVRLDEAAAFAHVVLQQPGLL
                     LEGIYTHIPFSDDQTGAWSQRRLAAFTSLVREIQTSHGIVILYAQGAASSVMSQNFPD
                     ELNTIAPGHLVFGLSPISGLRAESLGFRKALRALKASLIHIGHRRRGDDLIGSPTGGL
                     ASDATTGVVLFGMDNGFRPAAAGQTAMMLCHGQRCPVLSVSAEYAVIDVSQLEHPRVG
                     DEVTIIGTDGRDAIAVEDVALWQGAPSAAYWQVALRNVPFAAV"
     gene            complement(2805..3608)
                     /locus_tag="AC16A0_04345"
     CDS             complement(2805..3608)
                     /locus_tag="AC16A0_04345"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3104592.1"
                     /translation="MPSALLSEYETQGFAFRPGLLSTEEAGIITGELERIIARDGADP
                     QRIIMEKDGVTPRTVVNPHVHNEVFDRLVRHPALVNAAEELLGEEVYVFQSGVNCKAA
                     FNGDVWFWHQDYPAYYRDDHIPLPRMVNALIFFDDVSSMNGPLMMVPGSHQLTDDMPE
                     NTDQGTSYSFRDTSPDVIKEQVRLRGVVAPTGPAGSVIFMNVNVLHGSAGNLSPWSRR
                     MMTLTYNAMSNKATSPSIRPRHIVYEDRDLPPIKAMKGDCLTSLAGASV"
     gene            complement(3714..>4301)
                     /locus_tag="AC16A0_04350"
     CDS             complement(3714..>4301)
                     /locus_tag="AC16A0_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3104593.1"
                     /translation="AILAGKLRGTITRDQERELVGALIEAPRHISEILKDEKHIAAVA
                     KEIAQASDVLFLGRGINYPIALEGALKLKEISYIHAEGYAAGELKHGPIALIDEAVPV
                     IVIAPHDDLFDKTVSNMEEVAARGGRIVLITDEEALASAGRGAFQQIRVPRAHPFISP
                     LLYAVPVQLLAYHAAVVRGTDVDQPRNLAKSVTVE"
BASE COUNT          753 a         1357 c         1339 g          852 t
ORIGIN      
        1 tttcggacat cgcctcaggc cggattctga ctggtctgct ctggctcttg ccttcccaca
       61 gcgtccttgg caatcaatag ttatgacgcc gcttcccgca gcacgattca cgatcggcca
      121 ggtggtcaag caccggctgt tcccctttcg gggcgtgatc ttcgatgtcg accccgtgtt
      181 caacaacacg gaggactggt ggctgtccat tccggaggaa atccgcccgc gcaaggacca
      241 gcctttctac catctgttgg cggagaatga cgacacggag tacgtggcct acgtctcgga
      301 gcagaacctt ctgatcgacg acaccgggga gcccatacgt catcctcagg tccgggagta
      361 cttcgaaggc ttcgacccca agcggaagag ctaccggccc cgccgccgca tcagtcatta
      421 gcgcgtcaac cgcaactgcc agatcaggac agcaatgacc gcaaggccac caggacgcca
      481 tgccgtgacc tttgtcttca tcaccgtgat gatggacatg atcggctttg gcctcgtgat
      541 gccggtcctg ccgcggctca tcgaggaggt gggcggcact gacctcgccg gggcatcgct
      601 gatcgcggga tggatgtatt ttgcctatgg cggcatgcag tttctcttcg gtccggccgt
      661 cggcaatcta tcggatgcct atggccggag acctctgctg ctgctgtcgg tcttcggcct
      721 ggccgtcgac ttccttttca ccgcactggc gccgaccctg ttcctgctct tcgtcgggcg
      781 cgtcgcggcc ggtatctgcg gcgcgtccta caccatcgcc aatgcctatc tggctgacat
      841 caccaagccg gaggaccggg cccgggtctt tggcctgatg ggagcggcct tcggtctcgg
      901 cttcgtcatc ggcccggcca tcggcggatt gctcggcgag ttcggcccac gggtgccgtt
      961 cttcgtggcg gcggcgctga ccggcatgaa cttcgtcttc ggctatttcg tgctgccgga
     1021 gacactgccg cctgaccaga ggaggccctt ctcgctgcgt cgatcaaatc ctgtcggtgc
     1081 gctgaaggtc ttcgccagct accgcggcgt ggtgccgctg gcagccgtga tgtttgccta
     1141 tttcctcgca acgtctgtct accccgccat ctgggccttc tggggcatcg cccgcttcgg
     1201 ctggagcgag gcaatgatcg gcctgacgct ggccgccttt ggactgatca cagccatcat
     1261 tcagggcacg ctcacgggtc cgaccgtcaa gtggctgggc gagaagaaca ctgtcatctt
     1321 tggtctcgcg gcgtccgtca tcgcagcggc aggctatgtc tgggcgccgg ggcttctcgc
     1381 cgtcatggtg ctgctggtca tccatgcgcc cgagggcttt gtccatccgg ccctgacggc
     1441 gatgatgtcg aagtcagctc cgccagacgc gcaaggagaa ctgcaaggcg gcattgccag
     1501 cctgcagagt atcgccatgc tgatcggcac agtgttcttc gcccaggtct tcggctggtt
     1561 catgcagccg acgtccccga tcatcacgcc cagtgcaggc tatgtcatcg cggccgtcat
     1621 tcttgccggc gtgctcgcct tcttcctgct gctccggcag cagcgggtgg atgactagac
     1681 ggccgcgaag ggaacgttgc ggagtgctac ctgccagtag gccgcgctag gcgcaccttg
     1741 ccagagcgca acatcctcga ccgcaatggc atcccgtcca tccgtgccga tgatggtcac
     1801 ctcgtcaccc acgcgcggat gctcaagttg cgaaacatcg atgactgcgt attcggctga
     1861 cacgctcaga accggacacc gctggccatg gcagagcatc attgccgtct gccctgcggc
     1921 ggccgggcgg aatccattgt ccatgccgaa cagaaccacg cccgtcgtcg catcggacgc
     1981 caggccgccg gtcggcgacc cgatcagatc gtcgcccctg cgccggtgcc cgatgtggat
     2041 gagacttgcc ttgagggcgc gcaacgcctt gcgaaagccc aagctctctg cccgaagccc
     2101 ggagatgggg ctgaggccga aaacaagatg cccaggcgcg atcgtgttga gttcgtcggg
     2161 aaagttctgg ctcatcaccg aactcgcggc accctgggca taaagaatga caatgccatg
     2221 gctggtctgg atttcgcgta ccagacttgt gaaggcggcg agtcgccgct gtgaccaggc
     2281 gcctgtctgg tcgtccgaga aaggaatgtg cgtgtagatg ccctcaagca acaatccggg
     2341 ttgctgcaga accacgtgcg caaaggcggc agcttcgtcc agccgcactc ccagacgtcc
     2401 gagaccggcg tcaaccttga tatgcaccgg aaccggattc gcccgacgag atccgagttg
     2461 ggcaagaacc tgaagcgact cccgatcata gacgctgggc gtgaggcctt ggtccaacac
     2521 gtcaacatgc cccgatggca gctgcgcacc gtaaaggaga atggggatgg tgactcctgc
     2581 ccggcgtgcc gcaacggcat cgtcgaaatt cgccgtcgcc aggccttcga ctcccagctt
     2641 ctgcaggtgc aggctgacgg ctgccatgcc gtggccatag gcattggcct tgaccggcgc
     2701 caggatactg accggccggc cggcacgctc ccgcaacagg ccgacgttgt gtgttatggc
     2761 ggcaaggtca atctcgcccc agaccggacg cgcatcacga tggctcacac gctggctccg
     2821 gctagagacg tcaggcaatc acccttcatg gccttgatcg gcggaaggtc tcggtcctca
     2881 tagacgatgt gccgcggccg gatcgacggg ctcgtcgcct tgttcgacat ggcattgtag
     2941 gtgagcgtca tcatgcgccg ggaccagggc gagagattgc cggccgaccc gtgcagcaca
     3001 ttcacgttca tgaagatgac cgagcccgcc ggaccggtgg gcgccaccac gccgcgaagg
     3061 cggacctgtt ccttgatgac gtccggactg gtgtcccgga aggaatagga ggtgccctga
     3121 tcggtgtttt cgggcatgtc atcggtcaac tggtgagacc ccgggaccat catcagcgga
     3181 ccgttcatgc tgctgacatc atcgaaaaag atcagcgcat tgaccattcg gggcagcggg
     3241 atgtgatcgt cccggtagta ggctgggtag tcctgatgcc agaaccagac gtcaccgttg
     3301 aaggctgcct tgcagttcac gccggactgg aaaacgtaca cctcttcgcc gagcagttcc
     3361 tcggccgcgt tgacgagggc gggatgacgg accagccgat cgaatacctc attgtggaca
     3421 tgcgggttca cgaccgtgcg gggcgtgacg ccgtccttct ccatgattat cctctgggga
     3481 tccgcgccat cgcgggcgat gatccgctcc agttcgcccg tgatgatccc tgcctcctcc
     3541 gtcgacaaga ggcccggccg gaaggcaaag ccctgggtct catattctga gagaagtgca
     3601 gacggcatct ggagcccctt gatggtgttg atccacgtag gctatttgct ccttgtacag
     3661 ggggcaagag gctgtctggc agagcagtcg tgcaggccgc gcgggcggcg cttctattcg
     3721 accgtcacag acttcgccag attgcgcggc tggtccacgt ccgtaccccg gacgactgca
     3781 gcatggtagg ccagcagttg cacgggcacc gcatagagca gcggactgat gaagggatgg
     3841 gcgcgcggta cccgtatctg ctggaaggcg cccctgcccg ccgaggccag agcctcctca
     3901 tcggtgatca gcacgatcct gccgccgcgc gctgccacct cttccatgtt ggagacggtc
     3961 ttgtcgaaga gatcatcatg cggcgcaatg acgatgaccg gcaccgcctc gtcgatcaga
     4021 gcgatggggc catgcttcag ttcgccggca gcatagcctt cggcgtggat ataggagatt
     4081 tccttgagtt tcagcgcgcc ctcgagggca atgggataat tgatgccgcg gccgaggaag
     4141 agcacgtcgc tggcctgggc gatctccttg gccacggcag caatgtgctt ctcgtccttc
     4201 aggatctcgg agatatggcg cggtgcctcg atgagtgctc caaccaactc gcgctcttga
     4261 tcgcgggtga tggtgccgcg cagtttgccg gccagtatgg c
//
LOCUS       DBNGVR010000198         7210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_860731, whole genome shotgun sequence.
ACCESSION   DBNGVR010000198 DBNGVR010000000
VERSION     DBNGVR010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_860731"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="AC16A0_04355"
     CDS             complement(<1..984)
                     /locus_tag="AC16A0_04355"
                     /inference="COORDINATES: protein motif:HMM:NF021367.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HIR3104594.1"
                     /translation="MTRGVKSALGLTQVLLAAMVWVFAMTAGLQAGGPVETSIFAVQG
                     VDVDVTSTDAAAAKNQALLDVQVKAFFQLVGRLGTPELVAELQAKLKPEDITPYLRSL
                     SIEQESSAPGRYIGKFTVRFLPEPMNKLFAAYGIKVPSRQGDPILVLPVWRGKEANQM
                     WEDNIWRKAWLELRGEQGLVPLIVPLGDLEDTEAIDAEAALLKDPIKIEAIRRRYGAP
                     TLLLAQAQLAEGGGIHMFIEGETEFGKVSYNKIVTAEDGNPETAAMNGVQTFQNLLFK
                     AYTDNAAKVAAEEAARRANERQEIAVSVPFSSPTEWNSIRSRILAAPNVMGV"
     gene            1165..2250
                     /gene="purM"
                     /locus_tag="AC16A0_04360"
     CDS             1165..2250
                     /gene="purM"
                     /locus_tag="AC16A0_04360"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3104595.1"
                     /translation="MTSKGTATGNGISYADAGVDIAAGNALVDAIKPLARSTRRKGAD
                     SKLGGFGGLFDLKACGFRDPVLVAANDGVGTKLRIAIETGRHHGVGIDLVAMSVNDLV
                     VQGAEPLFFLDYYATGKLDVSAARDVIAGIADGCRQAGCALIGGETAEMPGMYEGDDY
                     DLAGFAVGAVERKMILPRKDVRPGDVILGLASSGPHSNGYSLVRKLVARSGLSYADPA
                     PFDAAQSLGEALLMPTRIYVKPLLKAIRAKAGIKALAHITGGGFIENIPRILPRATVA
                     DINLDQVPYLPVFLWMQETGGVAQREMLRTFNCGIGMVVVVAAEDAKKAASLLRRAGE
                     TVVTLGSIRRRRGQEEQVALSGALEVR"
     gene            2247..2840
                     /gene="purN"
                     /locus_tag="AC16A0_04365"
     CDS             2247..2840
                     /gene="purN"
                     /locus_tag="AC16A0_04365"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3104596.1"
                     /translation="MRRLRAAVLISGRGSNMMALAEAARAPDYPVTIACVISNRPDAE
                     GLAYAARHGIAAKLIDHREFASREGFEAALDRYLRAEAIDLIACAGFMRVLTGNFVAG
                     WEGRMINIHPSLLPAYKGLHTHERVLADGATEHGCTVHLVTAELDAGPIILQARVPVM
                     PDDTPETLAARVLTEEHRIYPQALAMLARDIQAKTST"
     gene            complement(2819..3532)
                     /locus_tag="AC16A0_04370"
     CDS             complement(2819..3532)
                     /locus_tag="AC16A0_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012555340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3104597.1"
                     /translation="MRRAERLLQLLQILRRHRRPVTGDAIARELEVSLRTVYRDIAAL
                     IADRVPIRGEAGIGYVLGEGFDLPPLMFTPDELEAVMLGLRWVARRGDADLSRAAQDT
                     VAKIGAVLPERLRPFLFDAALLVPPHFRLAEDQVNVALLRAAIREGRKAEIKYRSEDG
                     RETVRVIWPIAVAYFDAQRLIVAWCELRQDFRTFRTDRMLAMLVREEKYPARRKALLK
                     QWQDEAHVREGMPQVDVFA"
     gene            3723..4667
                     /locus_tag="AC16A0_04375"
     CDS             3723..4667
                     /locus_tag="AC16A0_04375"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR3104598.1"
                     /translation="MSKPLPIIIDTDPGQDDAVAILLALASPELEVLGITAVAGNVPL
                     ALTEKNARKICELAGRPDVKVHAGAIRPMVRSLVTAEEVHGKTGLDGPVLAEPTMALQ
                     DRHAVDYLVETLMARPAGTITLCTLGPLTNIGLALVREPRIASRIKQIVAMGGGYFEG
                     GNVTPSAEFNIYVDPHAARLVFEAGIPLTLIPLDCTHQALTTARRVEAFRAMNNKSGP
                     ATAAMLDFFERFDENKYGTDGGPLHDPCVIAWLLQPELFEGKLVNVSVECESELTMGM
                     TVVDWWRVTKRAPNATVVRNVNAEGFFKLLTERISRLP"
     gene            complement(4664..4918)
                     /locus_tag="AC16A0_04380"
     CDS             complement(4664..4918)
                     /locus_tag="AC16A0_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104599.1"
                     /translation="MMIPSTRILLLMVTALMVATAATAATAFAEGQPRKKKRIIPDME
                     QVADPIEQTSQASLKARTKRPSPNNIPPLTPGPDLLPTIN"
     gene            complement(5038..7128)
                     /locus_tag="AC16A0_04385"
     CDS             complement(5038..7128)
                     /locus_tag="AC16A0_04385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR3104600.1"
                     /translation="MNILPNIRTGISVCPHDCPSACALEVEVLDEHTIGRVRGAKANS
                     YTLGVICEKVGRYAERIHHPDRLLHPLKRKGPKGSGEFTRISWDDAMAETADALLKAE
                     AEYGAESVWPYFYAGTMGLVMRDGINRLRHAKRYSGMYDTFCVALSWSGYLAGVGHVS
                     GVDPREMQKSDLIVIWGGNPVNTQVNVMTHAMMARKNRGAKIACVDIYETGTMKQADV
                     RLLIRPGTDGALACAVMHVLFRDGLADWSYLERFTDAPREFEAHLKTRTPEWASKICD
                     IPVADIEAFAKLIGGTKRTYFRLGYGFTRGRNGAVNMHAVTCIPAVTGAWQYEGGGAL
                     HSNSGMYRWNKTMIEGLDVKSPDVRVIDQSRIGAALLGDPHDLAGGPPIKALFVQNTN
                     PASVAPDQTKVKQGLAREDLFTCVHEQFMTETAKFADIVLPATMFLEHDDLYQGGGHS
                     HIMLGRKLVEPPGECRNNHQVICDLAQRVGASHPGFEMSERELIDWTLTHSNRPGLTQ
                     LDAENWIDVQPDFEVAHNLKGFGFPDGKFRFRPRWGSVRFKGGGRSYGPVDRLPEFPD
                     HCDVIEEATAEYPFRLATSPARTFLNSSFTETPSSRKREGRPTVFVHPDDLRELGIED
                     GAKVRLGSARGTVTLHAKAHEGQRRGVLIAESIWPNESFEDGQGINTLTGADQPAPSA
                     GGCFHDNRVWIRLA"
BASE COUNT         1258 a         2347 c         2328 g         1277 t
ORIGIN      
        1 cacgcccatc acattgggcg ccgcgaggat ccgggacctg atgctgttcc attcggtcgg
       61 actggagaag ggcaccgaca cggcgatttc ctggcgttcg ttggcgcgcc tggcggcttc
      121 ctcggccgca acctttgcgg cgttgtccgt gtaggccttg aacaggaggt tctggaaggt
      181 ctggacgccg ttcatcgcgg ccgtttccgg attgccgtct tcggccgtga cgatcttgtt
      241 gtagctgacc ttgccgaact cggtctcgcc ttcgatgaac atgtgaattc cgccgccttc
      301 ggcgagttgc gcctgggcca gaagcaaggt gggcgcgcca tagcgccggc gtatggcttc
      361 gatcttgatc ggatccttca ggagtgcggc ttccgcgtcg atggcctcgg tgtcctcgag
      421 atcgccaagc ggcacgatga gcggcaccag cccctgctcg ccgcgcagct cgagccaggc
      481 ctttcgccag atattgtctt cccacatctg gttggcctcc ttcccgcgcc agaccggaag
      541 cacgagaatg ggatcgccct gcctgctggg gaccttgatg ccgtaggcgg cgaacagctt
      601 gttcatcggt tccggcagga agcgcaccgt gaacttcccg atgtagcggc cgggcgccga
      661 ggattcctgt tcgatcgaaa gcgaccgcag atagggcgtg atgtcctcgg gcttcagctt
      721 ggcttgaagc tcagccacga gttcgggcgt gccgagccgg ccgaccagct ggaagaaggc
      781 cttgacctgc acatcgagca aggcctggtt cttggccgcc gcggcgtcgg tgctggtgac
      841 gtcgacatcg acaccctgga cggcaaagat gctggtctcg accgggccgc cggcctgcag
      901 gcccgccgtc atggcaaaga cccagaccat ggccgccagc agcacctgcg tcaggccaag
      961 cgccgacttt actcccctcg tcaaaccaaa gtcctcggtt tccacaatct cacaccggac
     1021 gaaccggcgc gctttattac gcaagcgctt cgggctatgc acgcaatatc gcatatgcgg
     1081 ccaaagtgta agcgccttgg caaaccgcct gtgccctcgt taacaaccgg cataaccata
     1141 tcagaaataa ggcaaactgg cggcatgacc agcaaaggga cagcaacggg aaacgggatc
     1201 agctatgccg acgccggcgt cgacatcgca gccgggaacg ccctggtcga tgccatcaag
     1261 ccgctggcgc gctcgacgcg gcgcaagggc gctgattcga aacttggcgg atttggcggc
     1321 cttttcgatc tcaaggcctg cggctttcgc gacccggtgc tggttgccgc gaacgacggg
     1381 gtcggaacca agctgcgcat cgccatcgag acaggccgtc accatggtgt cggcatcgat
     1441 cttgtcgcga tgtcggtgaa cgacctcgtt gtgcaaggcg ccgaacctct gttcttcctc
     1501 gactactatg cgacgggaaa gctcgacgtc tcggccgcgc gcgacgtgat tgcgggcatt
     1561 gccgatggat gccggcaggc cggatgcgcc ctcatcggcg gcgagaccgc agagatgccc
     1621 ggcatgtacg agggcgacga ctatgacctc gccggctttg ccgtcggtgc cgtcgagcgc
     1681 aagatgattc tgccgcgcaa ggacgtgagg cccggtgacg tgatcctggg tctggcctcc
     1741 agcggaccgc actccaacgg ctactcgctg gtccgcaagc tggtggcgcg cagcggcctt
     1801 tcctatgcgg atccggcccc cttcgacgca gcgcagtcgc tgggagaagc cctgctcatg
     1861 ccgacgcgca tctatgtcaa gcccctgctc aaggcgatcc gggccaaggc cggcatcaag
     1921 gcacttgctc acatcaccgg cggcggcttc atcgagaaca tcccccggat cctgccgcgg
     1981 gccacggtgg ccgacatcaa tctcgaccag gtgccctatc tgcccgtttt cctgtggatg
     2041 caggaaaccg gcggcgtggc ccagcgcgag atgctgcgca ccttcaactg cggcatcggc
     2101 atggtggtgg tggtcgcagc agaagacgcc aagaaggccg cgagcctgct tcgacgcgcg
     2161 ggtgagacag tggtcaccct cggcagcatc cggcgccgca ggggccagga ggaacaggtg
     2221 gcgctcagcg gcgcccttga ggtgagatga ggcgcctgcg cgcggccgtg ctcatctcgg
     2281 ggcgcggctc gaacatgatg gcgctggccg aggcggcgcg cgcgcccgac taccccgtga
     2341 ccattgcctg cgtcatttcc aatcgcccgg atgcggaagg gcttgcctat gccgcgcggc
     2401 acggtatcgc ggccaagctg atcgaccacc gcgaattcgc cagccgcgag ggcttcgaag
     2461 cagcactcga ccgctatctg cgtgccgagg cgattgacct catcgcatgc gcaggcttca
     2521 tgcgcgtgct cacgggcaac ttcgtcgcag gctgggaagg ccggatgatc aacattcacc
     2581 cttccctgct cccggcctac aagggtctgc acacgcatga gcgtgtcctg gccgacggcg
     2641 cgacggaaca tggctgcacg gtgcatctgg tcacggccga gctcgatgcc gggccgatca
     2701 tccttcaggc cagggtcccc gtgatgccgg atgacacgcc ggagacgctc gcggcacggg
     2761 tgctgacgga agaacaccgg atctatccgc aggccctggc catgctggcc cgcgacattc
     2821 aggcgaagac atcgacctga ggcatgccct ccctgacatg cgcctcgtcc tgccattgct
     2881 tgagcagggc cttgcgccgg gccggatact tctcctcgcg caccagcatg gcgagcatgc
     2941 gatcggtccg gaaggtgcga aagtcctggc gcagctcgca ccaggccacg atcaggcgct
     3001 gggcgtcaaa ataggccacc gcgatgggcc agatgacccg caccgtctcg cgtccatcct
     3061 cactgcgata cttgatctcg gccttgcggc cctcgcggat ggcggcccgg agaagggcca
     3121 cattcacctg atcttccgca aggcggaaat ggggtgggac cagcagggcg gcgtcgaaga
     3181 gaaagggcct caggcgttcg ggcaggacag caccgatctt ggccacggta tcctgagcgg
     3241 cgcgcgacag gtcggcatcg ccgcgccgcg ccacccagcg caatccaagc atcacggctt
     3301 ccagttcgtc gggcgtgaac atcagcggcg gcaggtcaaa tccctcgccc agcacatagc
     3361 cgatgcccgc ctcgccccgg atcggcacgc ggtcggcgat caacgctgca atgtcgcgat
     3421 agacggtccg gagcgagacc tcgagttccc gcgcaatggc gtcgcccgtc accggccggc
     3481 ggtggcggcg gaggatctgc agcagctgga gcaggcgttc ggcgcgtcgc atgggtttct
     3541 tctagcgtag cgtggtgtca ggagttgtca gcagcttggc agcatgatcg gcaggcggca
     3601 agacctgcgc aaggcgtacc cggcggtcct gccaactgct gggtgaatgg cggccatcgc
     3661 ggcggaggat tgctgcggca ccggggctcc attaagagat tggggagtaa cgaggaacca
     3721 caatgagcaa gccgcttccc atcatcatcg acaccgatcc gggccaggac gacgccgttg
     3781 ccatcctgct ggcgctggcc tcgcccgaac tggaggtgct gggcatcacc gcggttgccg
     3841 gaaacgtgcc gctggcactc accgagaaga acgcgcgcaa gatctgcgag cttgccgggc
     3901 ggccggacgt caaggtccat gctggcgcca tccgccccat ggtccgcagc ctggtcacgg
     3961 ccgaagaagt gcatggcaag acgggcctcg acggccccgt gctcgccgag cccaccatgg
     4021 ccctgcagga tcggcacgcc gtggactatc tggtcgagac tctgatggcc cggccggccg
     4081 gcaccatcac gctgtgcacg ctggggccgc tgaccaacat tggcctggcg ctggtgcgcg
     4141 aaccgcgcat cgcctcgcgc atcaagcaga tcgtcgccat gggcggcggc tacttcgagg
     4201 gcggcaatgt cacgccctcc gcagagttca acatctacgt cgatccccat gcggcgcggc
     4261 tggtcttcga agccggcatt cctctgacgc tcattccgct ggactgcacg caccaggccc
     4321 tgacgacggc aagacgggtc gaggccttcc gcgcaatgaa caacaagtcg gggcctgcca
     4381 ccgcggccat gctcgacttc ttcgagcgct tcgacgagaa caagtacggc acggatggcg
     4441 gtccgctgca cgacccctgc gtgatcgcgt ggctgctgca gccggaactc ttcgagggca
     4501 agctggtgaa cgtctcggtc gaatgcgagt cggaactcac catgggcatg accgtcgttg
     4561 actggtggcg cgtcaccaag cgggcgccca atgcgacggt tgtccgcaat gtcaatgcgg
     4621 aaggcttctt caagctgctg accgagcgga tcagccggct gccctaattg atggtcggca
     4681 gcaggtcggg tcccggagtc agaggcggaa tgttgttggg ggagggacgc ttcgtcctgg
     4741 ccttgagact ggcctgagag gtctgctcga tcggatcggc cacctgctcc atgtctggaa
     4801 tgatccgctt cttcttccgc ggttgtccct cggcaaaggc cgtggccgcc gtcgccgcgg
     4861 tggcaaccat cagggctgtc accatcagca gcaggattcg ggtggagggg atcatcatcg
     4921 atacaacctt gggggtgccc ctgcgggcgg gaaatcgccg taacttgact cccttctagt
     4981 ccctcaaggc tgagcggttc ctgactcggc cgttcatctc gcgttcatct tggccgctca
     5041 ggccaggcgg atccagaccc ggttgtcgtg aaaacagccg ccggcgctgg gcgcgggctg
     5101 gtcggcgccg gtcagggtat tgatgccctg cccgtcttcg aaggattcat tgggccagat
     5161 cgactccgca atcagcaccc cgcggcgctg gccctcatgt gccttggcat ggagcgtcac
     5221 ggtgccccgc gcgctgccca gcctgacctt cgcgccatcc tcgatcccga gctcgcgcag
     5281 gtcgtcggga tgaacgaaaa cggtcgggcg cccctcgcgc ttgcgcgagg aaggggtctc
     5341 ggtgaagctc gaattgagga aggtccgggc cggactcgtg gccaggcgga aaggatactc
     5401 ggccgttgcc tcctcgatca cgtcgcaatg atccggaaac tccggcagcc tgtccaccgg
     5461 cccatagctg cggccgccgc ccttgaaccg caccgacccc cagcgcggcc tgaagcggaa
     5521 cttcccgtcc gggaagccga agcccttcag attatgcgcg acctcgaagt caggttgcac
     5581 atcgatccag ttctccgcgt cgagctgcgt caggccgggg cgattcgaat gcgtcagcgt
     5641 ccagtcgatc agttcgcgct cgctcatctc gaatcccgga tggctggcgc cgacgcgctg
     5701 cgccaggtcg cagatgacct ggtgattgtt ccggcactcg cccggcggct cgaccagctt
     5761 gcggccgagc atgatgtgcg agtggccacc gccctgatag aggtcgtcat gctcgagaaa
     5821 catcgtcgcg ggcagcacga tatcggcaaa ctttgccgtc tcggtcatga actgttcgtg
     5881 cacgcaggtg aagagatcct cgcgtgccag gccctgcttg accttggtct ggtcgggcgc
     5941 caccgaggcc gggttggtgt tctggacgaa gagcgccttg atcggcggac cgccggcaag
     6001 gtcatgcgga tcgccaagca gcgccgcgcc gatgcggctc tgatcgatga cgcgaacgtc
     6061 cggcgacttg acgtcgagcc cttcgatcat cgtcttgttc cagcgataca tgccgctgtt
     6121 ggaatgaagc gccccgccgc cctcgtattg ccaagcgccg gtgacggcgg gaatgcaggt
     6181 gacggcatgc atgttgaccg cgccgttgcg accgcgcgtg aagccgtagc cgaggcggaa
     6241 ataggtccgc ttcgtcccgc caatgagctt tgcgaaggct tcgatgtcgg cgacgggaat
     6301 gtcgcagatc ttcgatgccc actctggcgt gcgggtcttg agatgcgctt cgaactcgcg
     6361 gggcgcatcg gtgaatcgct ccagatagga ccagtctgca aggccatcgc ggaagagcac
     6421 atgcatcacc gcacaggcca gggcaccgtc cgtacccgga cggatcagca gcctgacatc
     6481 ggcctgcttc atcgtgcccg tctcgtagat gtcgacgcag gcgatcttcg ccccgcggtt
     6541 cttccgggcc atcatggcat gcgtcatgac attgacctgg gtgttcaccg ggtttccgcc
     6601 ccagatcacg atcaggtccg atttctgcat ctcgcgcgga tcgacgcccg agacgtgtcc
     6661 cacgccggca agatatcccg accacgacag cgccacgcag aaggtgtcgt acatgccgga
     6721 gtagcgcttg gcgtggcgca agcggttgat gccgtcacgc atgacgaggc ccatggtgcc
     6781 agcatagaaa tagggccaga cgctttcggc accatattcg gcttcggcct tcagcagcgc
     6841 atccgctgtc tcggccatgg cgtcatccca ggagatacgc gtgaactcgc ctgacccctt
     6901 cggtcccttg cgcttcagcg ggtggagcag ccggtcggga tgatgaatgc gttcggcgta
     6961 gcggcccacc ttttcgcaga tcacccccag cgtataggaa ttggccttgg cgccgcgcac
     7021 ccgcccgatg gtgtgctcgt caagaacctc gacttcaagc gcacaggccg agggacagtc
     7081 atgcgggcag accgagatgc ccgtcctgat gttgggaagg atgttcatgg cccgagacta
     7141 tcaagccgca ccgaacaagt caccctgcac ggcgagcccc tcgtgggcaa gcagccattt
     7201 cttgcgcgcg
//
LOCUS       DBNGVR010000199         2311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_861174, whole genome shotgun sequence.
ACCESSION   DBNGVR010000199 DBNGVR010000000
VERSION     DBNGVR010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_861174"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1330
                     /locus_tag="AC16A0_04390"
     CDS             173..1330
                     /locus_tag="AC16A0_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3104601.1"
                     /translation="MQLDDIVIQVGQRPVTLLELLLGAGGLALVLLIAAVILAWRAQA
                     SRRSEAIDAMRRTADLEYRLAELSGTLRGFAEQAQGTQMHLARTLDERLDQVSHRLGV
                     SLHDQTERTSQSLSQLNERLAVIDSAQKNLTALSSEMVTLKDILANKQARGAYGQGRM
                     EAIVRDGLHSSAYAFQGVLSNNTRPDCLIRLPDSALRLVIDAKFPLEAFNALKTAKGD
                     SDIKAAETQLRSDVLRHVKDISSKYLIAGETHESAIMFVPSEAVYAELYERFEDVIQK
                     AHRARVIIASPNVLMLLIQTMQAIFKDARMREQAGLIQIEVTRMLEDVNRLKDRVADL
                     QRHFGMANADLEKLGISADRVTKRGLRIESLDLTEPAAIAEAPKPRLVERG"
     gene            1406..>2311
                     /locus_tag="AC16A0_04395"
     CDS             1406..>2311
                     /locus_tag="AC16A0_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR3104602.1"
                     /translation="MKKTTVAALALGGLLAMSGLAQAAECGNSAKGFNTFLKGIKAEA
                     AAAGVGARGLSALDGVEYQPGIIKKDRAQSVFSQSFLQFQARMVSDRRITTGQAMMKK
                     HKATFDAVQKAYGVPAPVLISFWALETDFGQVMGDFPTIPSLATLAWDCRRPEKFREQ
                     LIGALVLYDKGDLELEDMVGAWAGEIGHTQFLPKEYNETAVDFDGDGHRNLKRSIPDA
                     MASSAALLIKHGWQPNQPWLQEVSVPKDMPWDQADIAIQHPRSQWAKWGVKAREGKLK
                     ADNFPASLLLPMGKDGPAFLAYENFT"
BASE COUNT          440 a          758 c          722 g          391 t
ORIGIN      
        1 gattgataaa gcagaggtca ggccgcgggg caggccccga atcccaacag acacgagaat
       61 tgaaccgagc ggctcctgtc gccgtcccga gccgcgcggt tcaagggccc gtcctctcct
      121 atccggaggg gacgggccac ttgtttcggc cgaatcaggc tagactcttc tgatgcaact
      181 cgatgacatc gtgatccagg tcgggcagcg gcccgtgacc ttgctggagc ttctgctggg
      241 cgcgggcggg ctggcgctgg ttctcttgat tgccgccgtg atcctggcct ggcgggccca
      301 ggcgtcacgc cgcagcgagg ccatcgacgc catgcgccgc accgccgatc tcgaataccg
      361 ccttgccgaa ctctcgggca cgctgcgcgg ctttgccgag caggcgcagg gcacccagat
      421 gcatcttgcc cggacgctcg acgagcgcct cgatcaggtg tcacaccgtc ttggtgtcag
      481 cctgcatgac cagaccgagc gcaccagcca gtccctgtcg caactgaacg agcgtctggc
      541 ggtcatcgac tcggcacaga agaacctcac cgccctgtcg agcgaaatgg tgacgctgaa
      601 ggacatcctg gccaacaagc aggcgcgtgg tgcctatggg cagggccgca tggaggcgat
      661 cgtgcgcgat ggcctgcatt ccagcgccta tgccttccag ggcgtgctca gcaacaatac
      721 caggccggat tgcctgatcc gccttccgga ttcggcgctg cgtctggtga tcgacgcgaa
      781 gtttccgctg gaggccttca atgccctgaa gacagcgaag ggcgacagcg acatcaaggc
      841 cgcggaaacc cagttgcgct ccgatgtgct gcgccatgtg aaggacattt ccagcaagta
      901 tctgatcgcc ggcgaaaccc atgaatcggc gatcatgttc gtgccgtcgg aggccgtcta
      961 cgccgaactc tacgagcgtt tcgaggacgt gatccagaag gcccaccgcg cgcgggtcat
     1021 catcgcctcg cccaatgtgc tgatgctgtt gatccagacc atgcaggcca tcttcaagga
     1081 cgcccgcatg cgcgagcagg cggggctgat ccagatcgag gtgacccgca tgctcgagga
     1141 cgtgaaccgg ctcaaggacc gtgtcgccga cctgcagcgc cacttcggca tggccaacgc
     1201 cgatctcgag aagcttggca tctcggcgga tcgcgtcacc aagcgcggcc tccgcatcga
     1261 aagcctcgac ctgaccgaac ccgccgccat tgccgaggca ccgaagccga ggctggtcga
     1321 gcgaggctga gggcgtggcc atcaatttga ccaacatcgc ccctatcgaa ttgacaaaac
     1381 ggcataatcc attgggagtc cacacatgaa gaagacgact gttgcagcgc tggcgcttgg
     1441 cggcctgctg gccatgtcgg gccttgcgca ggcggccgaa tgcggcaact ccgccaaggg
     1501 cttcaacacc ttcctgaaag gcatcaaggc cgaagcggcg gctgccggcg tgggcgcccg
     1561 cggcctttcg gcgctcgatg gcgtcgagta ccagccgggc atcatcaaga aggaccgggc
     1621 gcagagcgtc ttctcgcaga gcttcctgca gttccaggcc cgcatggttt ccgatcggcg
     1681 catcaccacc ggtcaggcca tgatgaagaa gcacaaggcc accttcgatg ccgtgcagaa
     1741 ggcctatggc gtgccggccc ccgtgttgat ttccttctgg gccctcgaga ccgatttcgg
     1801 ccaggtcatg ggggacttcc cgaccatccc gtcccttgcc acgctggcct gggactgccg
     1861 ccgccccgag aagttccgtg aacagctgat cggcgcgctg gtcctctatg acaagggcga
     1921 tctcgaactc gaagacatgg tgggcgcctg ggccggcgag atcggtcaca cgcagtttct
     1981 ccccaaggaa tacaacgaaa ccgccgtgga tttcgacggc gacggccacc gcaatctgaa
     2041 gcgcagcatt cccgatgcga tggcatcctc ggccgcgctg ctgatcaagc atggctggca
     2101 gcccaaccag ccctggctgc aggaagtcag cgtgccgaag gacatgccct gggaccaggc
     2161 cgacattgcc atccagcatc cgcgcagcca gtgggccaag tggggcgtga aggcgcgcga
     2221 gggcaagctc aaggccgaca acttcccggc gtccctgctc ttgcccatgg gcaaggatgg
     2281 cccggccttt ctcgcctatg agaacttcac g
//
LOCUS       DBNGVR010000200         2948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_662849, whole genome shotgun sequence.
ACCESSION   DBNGVR010000200 DBNGVR010000000
VERSION     DBNGVR010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_662849"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="iolE"
                     /locus_tag="AC16A0_04400"
     CDS             <1..794
                     /gene="iolE"
                     /locus_tag="AC16A0_04400"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007701421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HIR3104603.1"
                     /translation="ETGRRFPLDPRVLGPILDRYGISVCGGWFSGLLLDGDIAAEKDR
                     IRPQMELFKAMKAPCIVYGETAGTIQGDRSRPLATKRRLSEDEIKVYGRKMTAFGEWC
                     AEQGMPISYHHHMAAVIETEPELDLLMKHSGEGLPLLYDSGHMAFAGGDVLRVIDKHH
                     KRISHVHTKDVRMGVIDTLDRSRESFLDAVVKGAFTVPGDGSLDFEAIVKRLASHGYE
                     GWFVVEAEQDPKTAPPLKMAEVGHKELLRVMTAAGYTVVQGRDHG"
     gene            787..1908
                     /locus_tag="AC16A0_04405"
     CDS             787..1908
                     /locus_tag="AC16A0_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3104604.1"
                     /translation="MAEIGYGLIGTGFMGRAHAYAYRAAPAVFPDIPRVRLRGVADAD
                     AAAAARFATQYGFETSTGDWRRLLDDPAIHIISVTTPNILHKDMAIAAARAGKHVHCE
                     KPLAPSAGEAREMMEAAEAAGVKTQVGYNYIKNPALKLAREMIAGGELGEITGFRGIH
                     AEDYMANPDGPYVWRLDASGGPGVIADLGSHIIGMARFLLGPMTEVAADVETIVKSRP
                     KEPGSAERVKVEVDDIARMMLRFARGCGGTIEANWVATGRKMQLDFEVYGSKGGLVFT
                     QERFNELHLYRAGGTPRELGFTRIEIGPQHPPYVNFCVAAGHQLGFNDLKTIEAAEFL
                     GEVAGGPRSGPDFREAWEIQKVVETAVNAGRARQWLKIP"
     gene            1954..2814
                     /locus_tag="AC16A0_04410"
     CDS             1954..2814
                     /locus_tag="AC16A0_04410"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR3104605.1"
                     /translation="MSWTDDKKTAWTWYRGAWHKGNPMLMGPMTHAPWLGSCVFDGAR
                     AFEGTAPDLDQHCRRIVRSAESFGLKIVKRAGEIEELIREGIAKFPKDAELYLRPMFW
                     AADGFVDVDPEATEFSVSVYDSPIPAPTGFSITVSPFRRPSFEYAPTDAKAACHYPNS
                     ARAMREAKARGFDNAVMLDPLGHVAELATANIWLVKDGAAHTPVPNGTFLNGVTRQRV
                     IDLLRKAGVAVHERSIRWQEFFGADEVFSTGNYAKVMPVTRVGERHLQPGPVMAKARE
                     LYWDYAHGGR"
BASE COUNT          517 a          932 c         1022 g          477 t
ORIGIN      
        1 tggagacggg gcggcgcttc ccgctcgatc ccagggtcct ggggccgatc ctcgaccgct
       61 atggcatcag cgtctgcggc ggctggtttt ccggcctgct gctcgatggc gacatcgcgg
      121 cggagaaaga ccgcatccgg ccgcagatgg agctgttcaa ggcaatgaag gcaccctgca
      181 tcgtctatgg cgagacggcg ggcaccatcc agggcgaccg cagcaggccg ctggccacca
      241 agcgcaggct gagcgaggac gagatcaagg tctatggccg caagatgacc gccttcggcg
      301 aatggtgcgc cgagcagggc atgccgatct cctatcacca tcacatggcc gcggtgatcg
      361 agaccgagcc ggagctcgac ctgctgatga agcactcggg cgaggggctg ccgctgcttt
      421 acgactccgg ccacatggcc ttcgcgggcg gcgacgtgtt gcgcgtgatc gacaagcatc
      481 acaagcgcat cagccacgtg catacgaagg acgtccgcat gggcgtgatc gatacgctcg
      541 accgcagccg cgagagcttt ctcgatgccg tggtgaaggg cgccttcacc gtgccgggcg
      601 acggctcgct tgacttcgag gccatcgtca agagacttgc aagccatggc tatgaaggct
      661 ggttcgtggt cgaggccgag caggacccga agacggcgcc gccgctgaag atggcggagg
      721 tgggccacaa ggagctgctg cgcgtgatga cggcggcggg ttacacggtt gtccagggga
      781 gagatcatgg ctgagatcgg atatggactg atcggcacgg gattcatggg cagggcccat
      841 gcctatgcct atcgcgccgc gccagcggtg ttccccgaca tcccgcgcgt gcgcctgcgc
      901 ggcgtggcgg atgcggacgc ggctgcggcg gcccgctttg ccacgcaata tggcttcgag
      961 acctcgaccg gcgactggcg ccgtctgctc gacgacccgg caatccacat catctcggtg
     1021 accacgccca acatcctgca caaggacatg gcgatcgccg cagccagggc cggcaagcat
     1081 gttcattgcg aaaagccgct ggcgcccagc gctggcgagg cgcgcgagat gatggaggcg
     1141 gccgaggcag cgggcgtcaa gacgcaggtt ggctacaact acatcaagaa cccggcgctc
     1201 aagctggcgc gcgagatgat cgccggaggc gagctgggcg agatcaccgg cttccgcggc
     1261 atccatgccg aggactacat ggccaatccg gacggcccct atgtctggcg cctcgatgcc
     1321 agcggcgggc cgggcgtgat cgccgacctc ggttcgcaca tcatcggcat ggcgcgtttc
     1381 ctgctgggtc ccatgaccga ggtggcagcc gacgtcgaga ccatcgtgaa atcgcggccg
     1441 aaggagccgg gttccgccga acgcgtgaag gtcgaggtcg acgacatcgc ccgcatgatg
     1501 ctccgttttg cgcggggctg cggcggcacc atcgaggcca actgggtggc gacgggccgc
     1561 aagatgcagc tggacttcga agtttacggc tcgaagggcg ggctggtctt cacccaggag
     1621 cgcttcaacg agcttcatct ctacagggcc ggaggcactc cgcgcgaact gggcttcacc
     1681 cgcatcgaga tcggcccgca gcatccgccc tatgtgaatt tctgcgttgc cgccgggcac
     1741 cagctgggct tcaacgacct caagaccatc gaggccgccg agttcctcgg cgaagtggcg
     1801 ggcggcccga ggtccgggcc tgacttccgc gaggcctggg agatccagaa ggtggtggag
     1861 acggccgtga atgccgggcg ggcgcggcag tggctgaaga ttccctgacg ccgcctgtcc
     1921 cgtggcaggc agcgtcgacc tggagatgtt ccgatgagct ggacggacga caagaagacc
     1981 gcatggacct ggtatcgcgg cgcctggcac aagggcaatc cgatgctgat ggggccgatg
     2041 acccatgcgc cctggctcgg ctcctgcgtc ttcgacggcg cgcgcgcatt tgaaggcaca
     2101 gcccctgacc tcgatcagca ctgccggcgc atcgtgcgct cggccgagag cttcggcctc
     2161 aagatcgtca agcgcgccgg cgagatcgag gagctgatcc gcgaggggat cgcgaagttt
     2221 ccgaaggatg cggagctcta tctgcggccg atgttctggg cggccgacgg attcgtcgac
     2281 gtcgatcccg aggccaccga gttttcggtc tcggtctatg actcgcccat tccggcgccc
     2341 acgggtttct cgatcaccgt ctcgcctttc cgccggccat ccttcgaata tgcaccgaca
     2401 gatgccaagg cggcttgtca ctatcccaat tcggcgcgtg ccatgcgcga ggcgaaggcg
     2461 cggggcttcg acaacgcggt gatgctggac ccgttgggcc acgtggcgga actcgccacc
     2521 gccaacatct ggctggtgaa ggatggcgcc gcgcatacgc cggtgcccaa cggcaccttc
     2581 ctcaacggcg tgacgcgcca gcgcgtgatc gacctgttgc gcaaggccgg cgtggcggtg
     2641 catgagcgga gcatccgctg gcaggaattc ttcggggccg acgaggtctt ctcgaccggc
     2701 aactatgcga aggtcatgcc ggtgacgcgc gttggcgagc ggcatctgca gcccggtccg
     2761 gtgatggcca aggcccgcga gctgtattgg gactacgcgc atgggggacg gtgagcggaa
     2821 agcaaggcga ctgccaccct cgctggcgct cgggccctca tccgcccttc gggcaccttc
     2881 tcccacctgc ggtgggagaa ggggtaaacc ggacatcctc gtcccgcttg cagagggaga
     2941 agggggaa
//
LOCUS       DBNGVR010000201         5443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4197903, whole genome shotgun sequence.
ACCESSION   DBNGVR010000201 DBNGVR010000000
VERSION     DBNGVR010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4197903"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..928
                     /gene="ypfJ"
                     /locus_tag="AC16A0_04415"
     CDS             65..928
                     /gene="ypfJ"
                     /locus_tag="AC16A0_04415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF016143.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR3104606.1"
                     /translation="MRRVNVPMGGRGGFSIKTIIFLVIIYFVLKMLGIDLLGGLNPGQ
                     QSQFDVPDVSTDVVQDSGPGAGTGPATADSDAGRNFVSRVLGSTERVWSDIFKNMNRD
                     YPEPKLVLFSGFVQSACGMAQSAMGPFYCPRDQKVYIDLSFYQDMKNKLGAPGDFAQA
                     YVIAHEVGHHVQNVIGIADKVMAERMRTSEERANALSVRMELQADCFAGLWAKEADAT
                     AQILEDGDIEEALNAAAAIGDDRLQKRSQGYVVPESFTHGSSAQRVRWFKQGYTANDL
                     RDCDTFGTDNL"
     gene            1043..1453
                     /locus_tag="AC16A0_04420"
     CDS             1043..1453
                     /locus_tag="AC16A0_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3104607.1"
                     /translation="MKKILCAIDGTEHSFHAIELAASLAAKFGASLAVCAVNVARGGA
                     RGPLIYAWEDDQVKKILDGAAERAKKSGATKVEELALRSREAAAAVVQYAEENGYDHI
                     VVGTGDKRGVSRLVLGSVAADISGRAHCSVTVAR"
     gene            complement(1470..1712)
                     /locus_tag="AC16A0_04425"
     CDS             complement(1470..1712)
                     /locus_tag="AC16A0_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104608.1"
                     /translation="MDLKPIPAVPDAETWDDDLFAPPLLVQVAIGVIVIALAWGGLLS
                     PRTQQAPDFCRYAETETSKQVGHAPGRQNQAGVYCS"
     gene            1890..3137
                     /locus_tag="AC16A0_04430"
     CDS             1890..3137
                     /locus_tag="AC16A0_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR3104609.1"
                     /translation="MEPRLVLREVFDAAVRAADPAAAVAAHLPQPPKGRTVVIGAGKA
                     AIPMAASLEQNWSGPLEGFVVAPHGYRHELKRIGVHFASHPVPDDASLAAADRALALA
                     GTLGPDDLLIALISGGGSALMSKPVPGIDLKDKLDLVRALLKCGANIAELNCVRKQVS
                     GVKGGRLARAAGRARLHTLVVSDVPGDDPAIVASGPTVADPTRKRDALEILNRYRIAI
                     PGAVRAWLEGPDDDRSLAEADRQVHVVVSPHESFRAARRRAEALGLQVLYLGDRIEGE
                     SREVAKVHAGIALEIAQYRQPVAPPCVILSGGETSVTVRGKGRGGRNVEFALALAVAL
                     AGERKIHALAADTDGIDGMEPTAGAFVDPDSLDRARARGLDPIAFLEGNDAHRFFEAL
                     GDQLVTGPTHTNVNDFRAILVAP"
     gene            3134..4054
                     /locus_tag="AC16A0_04435"
     CDS             3134..4054
                     /locus_tag="AC16A0_04435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3104610.1"
                     /translation="MKAQPPLIATACRYFMVVAETGSVRAAARHLNVAASAISRQLIL
                     LEEQLGHSLFDRSGRSLSLSPAGEVLLRGLRGLSQGTEATLDQLDALKGLKRGTVRLA
                     TVESISVSILPDLLLAFARHYPGIQVTVTVAGSDAVTGLVRDVHADLGFTFNPSSLEG
                     LEAIVVRDMHLGAVMAPSHPLAGSQSLTLAECLAHPIAWPSPGLSLRAALDKIPGTRG
                     LRPAFECNSLRLMASLARHGSCIAFQTPIGIEQEMAEGKLVWIPLSDKRLPLDRLMLV
                     RRRGPRGRVAADAFADLVREHVPHQGAVRR"
     gene            4173..4523
                     /gene="uraH"
                     /locus_tag="AC16A0_04440"
     CDS             4173..4523
                     /gene="uraH"
                     /locus_tag="AC16A0_04440"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033971 - hydroxyisourate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HIR3104611.1"
                     /translation="MGRLTTHVLDTASGKPAAGLKIELYMLDGGTVYLKTIHTNSDGR
                     ADGPLLEGAEFARGHYELRFHAGDYLRRAGADLPEPAFLDVIPIRFGIANPSQHYHVP
                     LLLSPYGYSTYRGS"
     gene            4527..>5443
                     /locus_tag="AC16A0_04445"
     CDS             4527..>5443
                     /locus_tag="AC16A0_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3104612.1"
                     /translation="MASRSAIRFLRRGQLVSLENVPPMRTLLDYLRLEEKSRGTKEGC
                     NEGDCGACTVALGSLRDGRLVYEPVNACILLMGQIDGKELVTVDDLAEDGTLHPVQQA
                     MVDLHGSQCGFCTPGFIMSLFALYHSGKAPTRQDIVDQIAGNLCRCTGYRPIVDAATR
                     ACTGTALDRWARTADETARKLAKLQDGTDLLCGSADSFIARPASADSLCYLAASNPEA
                     AIVAGATDVGLWITKQMRELPRIILVGGVEELHRISDKQGRLSIGAAATYAEAQAQLA
                     GLHPDIGEVLRRIGSRQVRASGTVGGNIA"
BASE COUNT          925 a         1762 c         1795 g          961 t
ORIGIN      
        1 cgtggacctg cggggccgag gcggtggcgg tggcttctga tttccgggcg gtggcggggg
       61 tggcatgcgc cgcgtcaacg tcccgatggg cggccgcggc ggcttcagca tcaagaccat
      121 catcttcctg gtgatcattt acttcgtgct caagatgctt ggcattgatc ttctgggcgg
      181 cctcaatcca ggtcaacagt cgcagttcga cgtgcccgac gtgtcgaccg atgtcgtcca
      241 ggactcgggc ccgggcgcgg gcaccggacc ggccacggcg gacagcgacg cgggacgcaa
      301 cttcgtctcg cgcgtacttg gatcgaccga acgggtatgg agcgacatct tcaagaacat
      361 gaaccgcgac tatccggaac ccaagcttgt gctcttttcg ggcttcgtgc agtcggcctg
      421 cggcatggcg cagtcggcca tgggcccttt ctactgtccg cgcgatcaga aggtctacat
      481 cgacctgtcc ttctaccagg acatgaagaa caagcttggc gcgccgggcg actttgccca
      541 ggcctatgtc atcgcccatg aggtgggcca tcacgtccag aacgtcatcg gcatcgccga
      601 caaggtgatg gccgagcgca tgcggaccag cgaggagcgc gccaatgccc tatcggtacg
      661 catggagctg caggctgact gcttcgccgg cctctgggcg aaggaggccg atgccacggc
      721 gcagatcctc gaggacggtg acatcgagga ggcgctcaat gccgccgccg ccatcggcga
      781 tgatcgactc cagaagcgct ctcagggcta tgtcgtgccg gagtccttca cgcacggcag
      841 ctccgcccag cgggtccggt ggttcaagca aggttacact gcaaacgatc tccgcgactg
      901 tgacaccttc ggcaccgaca atctctgagc gactggcgat tcagggtgtt tgactaagat
      961 caaggcttga tgcccccact gggccattag tggcactgca accacgggag ccgccggctc
     1021 tcgctctgga tggagacctg ccatgaagaa gatcctttgc gccatcgatg ggaccgaaca
     1081 ttccttccat gctatcgaac ttgccgccag ccttgccgcg aagtttgggg ccagcctggc
     1141 ggtctgcgca gtcaacgtgg cccgcggcgg cgcgcgtgga ccgctgattt atgcatggga
     1201 agacgaccag gtgaagaaga tcctcgatgg agcggccgaa agggcgaaga agtcgggcgc
     1261 cacgaaggtg gaagagctgg cgctccgcag ccgggaagcc gccgcagccg tcgtgcaata
     1321 tgccgaagaa aacggctatg accacatcgt cgtcggcacc ggcgacaagc gcggcgtgtc
     1381 acgcctggtg ctgggttcgg ttgcggccga tatctccggg cgcgcgcatt gctcggtcac
     1441 cgtcgcgcgg tgaggcggcg gaacgccgct cagctgcaat agaccccggc ctggttctgc
     1501 cggccgggtg cgtggccaac ttgctttgag gtttccgtct ccgcataacg gcagaagtcg
     1561 ggggcctgct gggtccgggg cgaaagcaac ccgccccatg ccagggcgat gacgatcacg
     1621 ccaatggcga cttgtaccag cagaggcggg gcaaagagat cgtcatccca ggtctcggca
     1681 tccggcacgg ccggaatggg cttcaagtcc atggcgctcc ctccacgaaa accacatgcg
     1741 cgtcccgccc cctagatgcg gcatttgggt ttacggtcga tgaacggcat cggtaaaagt
     1801 ctcgcaaagt ttgaccgaac tcaattcctg tcgccacccg tccgggcctc ggttatatgg
     1861 ggccggagcc agctggggaa cgggacgcca tggagccgcg cttagtactg cgtgaagttt
     1921 tcgatgcggc ggtcagggcg gccgatccgg cggccgccgt ggcggcgcat ctgccgcagc
     1981 cgccaaaggg gcgcaccgtg gtgatcggtg ccggcaaggc cgccattccc atggccgcat
     2041 ctctcgaaca gaactggagt ggtcccctcg aaggcttcgt ggttgcaccg catggctatc
     2101 ggcatgaact gaagcggatc ggagtgcatt tcgccagcca tcccgttccg gatgacgcga
     2161 gtctcgcggc ggccgaccgc gcattggcgc tcgctggcac gctcggaccg gatgacctgc
     2221 tcatcgcact catctccggc ggcggctcgg cgctgatgtc gaagccggtt ccgggcatcg
     2281 atctgaagga caagctggac ctggtgcggg cgctgctgaa gtgcggcgcc aacattgccg
     2341 aactcaactg cgtgcgaaag caggtctccg gcgtgaaggg cggccggctg gcaagggcgg
     2401 ccggccgggc acgcctccac acgctggtgg tgtcggacgt gcccggcgat gatcccgcca
     2461 ttgtcgcctc cgggcccacg gtggctgacc ccacccgcaa gcgcgatgcg ctggagatcc
     2521 tgaaccgtta ccgtatcgcc atcccggggg cggtgagagc ctggctcgaa ggcccggatg
     2581 acgatcgcag cctggccgag gcggatcgcc aggtgcatgt cgtggtctct ccgcatgagt
     2641 ccttccgcgc cgcgcgccgc agggccgagg cgctgggctt gcaggtgctc tatctcggtg
     2701 accggatcga aggcgaatcc cgcgaggtgg cgaaggtgca tgcaggcatt gccctggaga
     2761 ttgcgcagta ccgtcagccg gtggcgccgc cttgtgtcat tctctcgggc ggcgagacca
     2821 gcgtcaccgt gcgcggcaag ggccgtggcg gccgcaatgt ggagttcgcg ctggccctgg
     2881 ccgttgcgct ggcgggggag cggaagatcc atgcgctggc agccgatacc gatggcatcg
     2941 acggcatgga gccgacggcg ggggccttcg tcgatccgga cagcctggac cgtgcaaggg
     3001 ccaggggcct tgatccgatc gccttcctcg aaggcaacga cgcgcacagg ttcttcgaag
     3061 cgctgggcga ccagctggtc accggtccca cccacaccaa cgtgaacgac ttccgcgcca
     3121 tactcgtggc accatgaaag cgcagccgcc gctcatcgcc acggcctgcc gctacttcat
     3181 ggtggtggcc gagaccggtt cggtgagagc ggcggctcgc catctcaacg tcgctgcgtc
     3241 cgccatcagt cgccagctca tccttctcga agaacagcta ggtcattcgc tcttcgaccg
     3301 cagcggacgc agcctgtcgc tgtcgcctgc gggtgaggtc ctgctgcgcg gcctgcgtgg
     3361 cctttcccag ggtaccgagg caacgcttga ccagctcgat gcgttgaagg gcctcaagcg
     3421 cggcacggtg cggttggcga cggtcgaaag catctcggtg tcgattctgc ccgatctttt
     3481 gctggccttc gccaggcact atcctgggat ccaggtgact gtgaccgtgg cgggatcgga
     3541 cgcggtgacg ggattggtcc gcgacgtgca tgccgatctc ggcttcacct tcaatccgtc
     3601 ctccctggag gggctggagg ccatcgtcgt gcgcgacatg catcttggcg ccgtcatggc
     3661 gccctctcat ccgcttgcgg gatcgcaaag cctcacgctg gctgaatgcc tcgcgcatcc
     3721 catcgcctgg ccgtcgcccg gcctcagctt gcgggctgcg cttgacaaga tccctggcac
     3781 ccgcggcctg cggcccgcct tcgagtgcaa ttcgctccgc ctcatggcat cgctggcgcg
     3841 tcacggcagc tgcatcgcct tccagacgcc aatcggaatc gagcaggaga tggcggaggg
     3901 aaagctggtc tggattccgc tcagcgacaa gcgcctgccg ctggaccggc tcatgctggt
     3961 gcgccgccgc ggccccaggg gccgggttgc ggccgatgcg ttcgcggacc ttgtgcgcga
     4021 acatgtccca caccagggag cagtgcgaag ataacgtacg gtccgtagca attattcttc
     4081 tatttcgcac agacaaccgg tgttatcgtc ttcgccagac gcggcaccag aaccgcggac
     4141 accggaatgg gcaggggagc tgaggaagcg cgatggggcg gctgaccacg catgttctcg
     4201 acacggcctc gggaaagccc gcggcgggtc tgaagatcga gctttacatg ctcgacggcg
     4261 ggacggtcta tctcaagacg atccacacca attccgacgg gcgtgccgac gggcccctgc
     4321 tggaaggagc ggaattcgca cgcggccatt atgaactccg ctttcacgcc ggcgactatc
     4381 tgcgccgtgc cggagccgac ctgcccgagc cggctttcct cgatgtcatt ccgatccgtt
     4441 tcggaatcgc caacccaagc cagcattacc acgtcccgct tctgctatcg ccctacggtt
     4501 actccaccta tcgcggcagc tgatccatgg cctcgcgcag cgccatacgt ttccttcgcc
     4561 gcggccagct ggtctcgctc gagaacgtgc cgcccatgcg cacgcttctc gactatctcc
     4621 ggcttgagga gaagtcgcgc ggcaccaagg agggctgtaa cgaaggagac tgtggcgcct
     4681 gcacggtggc tctcggcagt ctgcgtgacg ggcgtctcgt ctatgaaccc gtgaatgcct
     4741 gcatcctgct gatgggccag atcgacggca aggagctcgt cactgtcgac gaccttgccg
     4801 aagatggaac gctgcatccg gtgcagcagg cgatggtcga cctgcacggt tcgcagtgcg
     4861 gcttctgcac gccaggattc atcatgtcgc tcttcgcgct ctatcattcc ggcaaggcgc
     4921 cgacacggca ggacatcgtc gaccagatcg ccggcaatct gtgccgctgc acgggctatc
     4981 gccccatcgt ggatgcggcc accagggcct gcaccggcac ggcgctcgac cgctgggcca
     5041 ggaccgcgga cgagacggcc cggaagctgg cgaagctgca ggacggaacc gatctgctct
     5101 gcggatcggc tgacagcttc atcgcacggc cggccagtgc ggactcgctt tgctatcttg
     5161 ccgccagcaa ccctgaggcg gccatcgtcg caggtgcgac cgacgtggga ctgtggatca
     5221 ccaagcagat gcgcgagctg ccgcgcatca tactggtcgg tggtgtcgag gagcttcacc
     5281 ggatctcgga caagcagggc aggctgtcga tcggcgccgc tgcgacctat gcggaggcgc
     5341 aagcgcaact cgcggggctc caccccgata tcggcgaggt cctgcgccgc atcggctcgc
     5401 gccaggtgcg tgcctccggc accgtcggcg gcaatatcgc caa
//
LOCUS       DBNGVR010000202         5338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4789167, whole genome shotgun sequence.
ACCESSION   DBNGVR010000202 DBNGVR010000000
VERSION     DBNGVR010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4789167"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="AC16A0_04450"
     CDS             <1..1271
                     /locus_tag="AC16A0_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3104613.1"
                     /translation="ALLMSLVSQVPSEFTLNARNPERTVKVGGRNTVFVPMYGAPYVR
                     DLDNTRRYGSLEDLNNFHKLSYMSPALHSSSSIICEPMEIPVPKRHLHIVHSALQHSD
                     KPFMGIVTAKERAEDVMKMAGIVFGDDYVKDNTVLVSITNCNSPLVWDATMLDAMKVY
                     ARHNQPLILAPFALCGASTAASAVGAVAQVNAEALAGVAFTQLVRPGSPQIYGQFMVT
                     VDMKSGAPMGGTPEAAQMMYLMGALARKYRLPWRTSGFHVGSKLNDAQAGYEANMLMH
                     AAILAGANYIWHVAGWLEAGLCCGYSKFVTDAEQLVGWYKYAGGLSFDDFKEAMAAVR
                     EVGPAGHFLGTQHTLEHFEKAFFIPSIMDFNSFEQWSAEGGKDHDTRGRDKARALLAA
                     YEEPRLDQGIAEGLRDFIARREEELPNSVS"
     gene            1295..1927
                     /locus_tag="AC16A0_04455"
     CDS             1295..1927
                     /locus_tag="AC16A0_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR3104614.1"
                     /translation="MLGDFITSAIATLLVVADPVFMSALFLGITNGMGKEQRREVALR
                     ASIIAFCILLAAGLGGAQLLNLLGISLSAFRIAGGLLLLSAAAEMVFDRRSQRLQATA
                     EQAITVDHVRNIAAFPLAIPLMAGPGAITAMILLAGRADGRIEYLVSLYVVAGLVMAA
                     CFACFLAAERISKIMGITGRAVLTRLLGIILAALAVQFIIDGVVALSPSP"
     gene            complement(1911..2201)
                     /locus_tag="AC16A0_04460"
     CDS             complement(1911..2201)
                     /locus_tag="AC16A0_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase inhibitor subunit zeta"
                     /protein_id="HIR3104615.1"
                     /translation="MSIEEIEMHTEGQVIGRRNLRLGLWAGMRLGLRGDRLTRYARDV
                     MDADYRLPGPHDVVQKIADDFEEHGVDYPTDMIMMEIQRIERQVRTELMATD"
     gene            complement(2212..2511)
                     /locus_tag="AC16A0_04465"
     CDS             complement(2212..2511)
                     /locus_tag="AC16A0_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104616.1"
                     /translation="MRNLVWIATAAVIALWSGVAWLLHAAIGLGGSLAATNADLIPAN
                     PELVEWASWFALFGTGVGEWLVIAIWALVSAVLLALGFVATRLLPRLSRSIAAQS"
     gene            complement(2511..2888)
                     /locus_tag="AC16A0_04470"
     CDS             complement(2511..2888)
                     /locus_tag="AC16A0_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HIR3104617.1"
                     /translation="MANAYPGTIHLDLTRDGTLARLEWLSWFTDSAIRIPGTTRSFGA
                     DGLLSIVPGAGSLAGTGISLYLLAEAIRHGAPLPLLARMGGNVAADMVVGAIPVVGFA
                     FDMVFKANQRNLRLLREHMKGNT"
     gene            3446..4885
                     /locus_tag="AC16A0_04475"
     CDS             3446..4885
                     /locus_tag="AC16A0_04475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR3104618.1"
                     /translation="MTRTDPSRINCDLCIIGAGSGGLSVAAAAAQMGARVVLVEAGAM
                     GGDCLNTGCVPSKAMIAAAQAAHGGEGAPELGIAAATPRIDFAAVHAHVRRVIAAIAP
                     HDSVERFESLGVQVVKAEARFTAPDEIIAGTQRITARRFVIAAGSRAALPPIPGLAEA
                     GAYTNETIFDLTERPEHLIVIGGGPIGLELSQAFRLLGSAVTVLERHVILPRDEPEAA
                     ELVRRRLREDGVDLRESIQIDSVRRDAAGVTVGIADRDGKRSIAGTHLLVAAGRQPNV
                     ERLGLEAAGIVFTPKGITVDAGLRTSNRKAYAIGDIAGGPQFTHIAGYHAGLVIRSAL
                     LGLPAKVDYAALPWVTFLDPEIAHVGLTEAEARKQHGSVTVLHESFARNDRAVTEQET
                     QGFIKVIAGKRGRILGATIVAPHAGEMISLWGLAISRRLKLSAIAGMLAPYPTLSEIS
                     KRAAGQYYAPVVFGSKVRALVRLVQKYLP"
     gene            complement(4886..>5338)
                     /locus_tag="AC16A0_04480"
     CDS             complement(4886..>5338)
                     /locus_tag="AC16A0_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR3104619.1"
                     /translation="KCPNVFKGYWRMREKTRDEFTADGYFITGVVATIDAEGRVAIVG
                     RAKDLIISGGFNVYPKEVEDVLDALPFVAESAVVGVPHPDFGEGVIAIVTAKGAVPEE
                     EAIIAQLAKQLAKFKVPKRIILVEALPRNAMGKVQKAELRRIYGDSFA"
BASE COUNT          925 a         1730 c         1706 g          977 t
ORIGIN      
        1 gcgccctgtt gatgagcctg gtgtcgcagg tgccgtccga gttcacgctc aatgcgcgca
       61 atccggaacg cacggtgaag gtgggcgggc gcaacaccgt tttcgtgccc atgtatggcg
      121 cgccttatgt gcgcgatctc gacaacaccc ggcgctatgg ttcgctggag gatctcaaca
      181 acttccacaa gctgtcctac atgtcgccgg cgctgcactc gtcgagttcg atcatctgcg
      241 aaccgatgga gattccggtg ccgaagcggc acctgcatat cgtgcattcc gcccttcagc
      301 actccgacaa gcccttcatg gggatcgtta cggccaagga acgtgccgaa gacgtcatga
      361 agatggcggg catcgtcttc ggcgacgact acgtgaagga caataccgtt ctcgtctcca
      421 tcaccaattg caattcgccg ctggtctggg atgccaccat gctggacgcg atgaaggtct
      481 atgcccggca caaccagccg ctcatccttg cgcccttcgc gctctgcggc gcctcgaccg
      541 cagcgtccgc ggtgggcgct gtggcccagg tcaatgccga agcgctggcg ggcgtcgcct
      601 tcacccagct tgtccggccc ggcagcccgc agatctacgg gcagttcatg gtgacggtcg
      661 acatgaagtc gggagctcct atgggcggaa cgcccgaggc cgcgcagatg atgtacctga
      721 tgggcgcgct ggcgcgaaag taccggctgc cctggcgcac ctccggcttc catgtcgggt
      781 caaagctgaa tgacgcccag gccggctatg aggccaacat gctgatgcat gcggcgatcc
      841 tggcgggtgc gaattacatc tggcacgtcg ccggctggct cgaggcgggg ctgtgctgcg
      901 gttactcgaa gttcgtgacc gacgcggagc agctggtcgg ctggtacaag tatgccggcg
      961 gactctcctt cgacgacttc aaggaggcca tggccgccgt ccgcgaagtc ggtcctgcag
     1021 ggcattttct cggcacgcag cacacgctgg aacacttcga gaaggccttc ttcattccca
     1081 gcatcatgga tttcaattcc ttcgagcaat ggtcggcgga aggcgggaag gatcacgaca
     1141 cgcggggccg cgacaaggcc cgcgccctgc tggcggccta tgaggagccc aggctcgacc
     1201 aaggcattgc cgaaggattg cgcgacttca tcgcgcggcg cgaagaggaa ctgccgaata
     1261 gcgtcagctg acggcgccgt gctaggctgg cgccatgctg ggcgacttca tcacttccgc
     1321 cattgcgaca ctgctggtcg ttgccgatcc cgtcttcatg agcgcgctct tcctcggcat
     1381 caccaatggg atgggcaagg agcagcgccg cgaggtggcg ttgcgcgcct cgatcatcgc
     1441 cttctgcatc ctgctggcgg cgggccttgg cggcgcccag ctgctgaacc tgctgggcat
     1501 ctcgctctcg gccttccgca ttgccggcgg gcttctcctt ctgtcggctg cggccgagat
     1561 ggtcttcgac cgccgcagcc agcggctcca ggccacggcc gagcaggcca tcaccgtcga
     1621 tcatgtgcgg aacattgcag ccttcccgct ggcgatcccg ctcatggcag gccccggcgc
     1681 catcacggcg atgatcctgc tggccggtcg cgccgatggc cgcatcgaat atctcgtctc
     1741 gctctatgtg gtcgccgggc tcgtcatggc ggcctgcttt gcctgcttcc tcgcggcgga
     1801 acgcatctcc aagatcatgg gcatcaccgg ccgcgcggtg ctgacgcggc ttctcggcat
     1861 catcctcgcc gcgctggccg tgcagttcat catcgatggc gtggtggcgc tcagtccgtc
     1921 gccatgagtt cagtcctcac ctgacgctcg atccgctgga tttccatcat gatcatgtcg
     1981 gtgggatagt cgacgccatg ttcctcgaag tcatcggcga tcttctgcac cacgtcatgc
     2041 ggtccgggca ggcgatagtc ggcgtccatc acgtcgcgcg catagcgcgt cagccggtcg
     2101 ccgcgcagtc cgaggcgcat gccggcccac aggccgagcc gcaggttgcg ccgtccgatc
     2161 acttgccctt cggtgtgcat ttcgatttcc tcgatcgaca tcagtttctc ctcatgattg
     2221 tgcggcgatg ctgcgcgaca ggcgcggcag caggcgcgtg gcaacgaaac ccagggccag
     2281 caggacggcc gagaccaggg cccagatggc aatgaccagc cactcgccca cgccggttcc
     2341 gaacagagcg aaccacgaag cccattccac caactccggg ttggccggaa tgaggtcggc
     2401 attggtggcg gccaggctgc cgcccaggcc gatcgcggcg tggagcagcc aggccacgcc
     2461 cgaccacagg gcgatgacgg ccgcagtcgc gatccagacg agatttcgca tcaggtgttt
     2521 cctttcatgt gttcgcgcag aaggcgcagg ttccgctggt tggccttgaa gaccatgtcg
     2581 aaggcgaagc cgaccaccgg gatggcgccc acaaccatgt cggcggcgac attgccgccc
     2641 atgcgcgcaa gcagcggcag gggcgcgcca tgccgtatgg cttccgcgag gaggtagagc
     2701 gagatgccgg tgcccgccag gctgccggcg ccgggaacga tgctgagcag tccgtcggcg
     2761 ccgaaggacc gggttgtgcc cggtatgcgg atggcgctgt cggtgaacca ggacagccat
     2821 tcaagccggg ccagcgtgcc gtcgcgcgtc aggtcgaggt ggatcgtgcc ggggtatgca
     2881 ttcgccatga ctgtctccca gattgcggca ggtatctgcc ttcaaggtgg aagagtccga
     2941 catcgcagtt tgggctgcca gaggggcccg gacccgttgc cgaggatatg ggggtgccgg
     3001 gataccattc aatgccgcac cggattaaaa cttggagagg aaaaagaggg gtgagcggca
     3061 gcaaccagaa accgctcacc ccaagcttgg ccgcccgccg caaggcggtt ggtcaagacg
     3121 ccacgaggac tctggacttg agaatgggag gacccaagac agacgtgtgc gttgcaacaa
     3181 gagatgcaag gggggtgcca gtttggaatc tgatttgtat tatttttcaa agcgaggaaa
     3241 ttattgagat attccggttt tcgtgaaggg tgatgcttga ccctgagtca ctctggatgc
     3301 aaggccattg tgcagtgcga tgcacaagaa ggcagcatct cggcttgtct gactgcgaat
     3361 cgtccagcct ttcgatcaca gggcgtcacg caatatctcg ggaaggtggc tggcaaagag
     3421 agccatgatg ggcgaaagga acggaatgac ccgcacggat ccgtcccgca tcaactgcga
     3481 cctctgcatc attggcgccg gctccggcgg actctccgtg gccgccgcag ccgcgcagat
     3541 gggtgcccgc gttgtgctgg tcgaagccgg cgccatgggc ggcgactgcc tcaacacggg
     3601 ctgcgtgccc tcaaaggcga tgatcgccgc agcccaggct gctcatggcg gggagggtgc
     3661 gccggaactc ggcattgcgg cggccacgcc ccgcatcgac ttcgccgcgg ttcacgcgca
     3721 tgtccggcgg gtcatcgcgg cgatcgctcc gcacgacagt gtcgagcgct tcgagtcgct
     3781 tggcgttcaa gtggtgaagg cagaggcgcg ctttaccgca ccggacgaga tcatcgccgg
     3841 cacccagcgg atcacggccc ggcgcttcgt catcgccgcg ggctcccgcg ccgcattgcc
     3901 gcccattccg ggtctggccg aagccggcgc ctacaccaat gaaaccatct tcgatctgac
     3961 cgaaaggccc gaacacctca tcgtcatcgg cggcggcccg atcgggcttg aattgtcaca
     4021 ggccttccgg ctcctcgggt ccgccgtgac cgtgctggag cgccacgtca tcctgccgcg
     4081 cgacgaaccc gaggctgcgg aactggtccg gcgccgtctg cgtgaggacg gcgtcgacct
     4141 gcgcgagtcg atccagattg actccgtgcg ccgcgatgct gctggcgtca cggtcgggat
     4201 cgcggacagg gacggcaagc gatccatcgc gggcacccac cttctggtcg cggctggccg
     4261 acagcccaat gtcgaaaggc tgggactgga ggcagccggc atcgtcttca cgcccaaggg
     4321 cattaccgtc gatgccggcc tgcgcaccag caaccgcaag gcctatgcca tcggcgacat
     4381 tgccggcggc ccgcagttca cgcacatcgc aggctatcac gccggcctcg tcatccgcag
     4441 cgccctgctg ggccttcccg caaaggtcga ctatgcggcg ctgccctggg tcaccttcct
     4501 cgatcccgag atcgcccatg tcggattgac cgaggccgaa gcgcgcaagc agcatgggtc
     4561 cgtcaccgtt ctgcacgagt ccttcgcccg caacgaccgc gccgtcaccg agcaggagac
     4621 ccaaggattc atcaaggtca tcgccggaaa gcgcggccgc attctcggtg caaccattgt
     4681 tgctcctcat gccggcgaaa tgatctcgct ctggggcctt gccatatcga ggaggctgaa
     4741 gctctcggcc atcgccggca tgctggcgcc ttatccgacc ctctcggaga tttccaagcg
     4801 ggcagcgggt cagtactacg cgccggtggt cttcggctcc aaggtccgcg ccctggtcag
     4861 gctcgtgcag aagtatctgc cctgatcagg cgaagctgtc gccgtagatt ctgcgcagtt
     4921 cggccttctg caccttgccc atggcattgc gcggcagcgc ctccaccaag atgatgcgtt
     4981 tcgggacctt gaacttcgcc agctgcttgg ccagctgggc gatgatcgcc tcctcttccg
     5041 gcaccgcgcc tttcgcggtc acgatggcga tcacgccttc gccgaagtcg ggatggggaa
     5101 cgccgacgac ggccgattcc gcgacgaagg gcagggcatc cagcacatcc tccacttcct
     5161 tcggatagac attgaagccg cccgagatga tcaggtcctt ggcgcggccg acgatggcca
     5221 cccggccttc ggcgtcgatg gtggcgacga cgccagtgat gaagtacccg tccgcagtga
     5281 actcatctct ggtcttttcc cgcatgcgcc agtagccctt gaagacgttg ggacactt
//
LOCUS       DBNGVR010000203         4226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_74038, whole genome shotgun sequence.
ACCESSION   DBNGVR010000203 DBNGVR010000000
VERSION     DBNGVR010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_74038"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /gene="aspA"
                     /locus_tag="AC16A0_04485"
     CDS             complement(<1..233)
                     /gene="aspA"
                     /locus_tag="AC16A0_04485"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR3104620.1"
                     /translation="MTLTRTERDSIGEWPVPHDADFGIHSARAADNFPITGILLRDYP
                     EFVQSLAMVKKAAAMANRDLGALQPVIADAIIAA"
     gene            395..1492
                     /locus_tag="AC16A0_04490"
     CDS             395..1492
                     /locus_tag="AC16A0_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3104621.1"
                     /translation="MDRRNFIRSAGVAAAAVPALAAPAIAQTMPEIKWRMTSSFPKAL
                     DTIYGAAETFARYVAEATDNRFQIQVFPAGEIAPGLEAANEVGKGSIEMCHTASYYYW
                     GKDPTYALATAVPFGLNARQQNAWMYYGGGVDLMNEFYATQNLIGFPCGNTGAQMGGW
                     FRKEINSVADLQGVKMRIGGFGGKVISKIGVVPQQIPGGEIYPALEKGTIDAAEWVGP
                     YDDEKLGFYKVAKYYYYPGWWEGGAMLHTMVNLAKWGELPPAYQAIVKSASQAANCDM
                     MASYDAKNPTAIKSLVANGAVLRPYPQDVMEACFNAANETYAEINASNAAFKKIYDSM
                     AAYRRDAYLWLQVSEATYDNFMMAQQRKKAL"
     gene            complement(1546..3204)
                     /locus_tag="AC16A0_04495"
     CDS             complement(1546..3204)
                     /locus_tag="AC16A0_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3104622.1"
                     /translation="MIELIAHNLAPIMFLSLIAFLLLGYPVAFSLAANGLLFFVIAVE
                     LSPYSNEIALDWPLLQTLPGRVLDVMRNDTLLAIPFFTFMGIILEKSGMAEDLLDTIG
                     QLFGPIRGGLAYAVILVGALLAATTGVVAASVIAMGLISLPIMLRYGYDRPLATGVIA
                     ASGTLAQIIPPSLVLIVLADQLGRSVGDMYAGAMIPGLMLAGLYCAYVFFVSVVSPQK
                     VPALPLAARTLKGSPAPLLAILAGTLVIGLAAYWLLARQMETGAGIWAATIATVATYL
                     FALADKHLKLGRLSHLVQQVVIVLVPPLGLIFLVLGTIFLGIATPTEGGAMGAAGALL
                     MAIAKRRLSFRLLSDALDATTRLSCFVLFILIGSRIFSLTFYGINGHVWVEELFLSVP
                     GGVMGFLIAVNILVFILGCFIDFFEIAFIVVPLLAPVAAKLGIDLVWFGVLLGVNLQT
                     SFLTPPFGFALFYMRSVAPVKDYIDKVTGGRIAGISTTQIYRGAVSFIILQAIMIAVT
                     VAFPWIALPDSGPKVDPNSIEIEIPAIETGGGESGGVPPPPAFD"
     gene            complement(3201..3779)
                     /locus_tag="AC16A0_04500"
     CDS             complement(3201..3779)
                     /locus_tag="AC16A0_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR3104623.1"
                     /translation="MGILLSISRMIDAVTRFIGYNVRWLILAAVLVSAGNAIIRKAFD
                     SSSNAWLELQWLLFGAVFMLAAAYTLQVNAHVRIDVVSSRLSKRTRDIIDAVCHILML
                     APLAAIMIWLSIPFFWDSYTGGEMSTNAGGLSVWPAKFLVLAGFVLLLLQMASELIKR
                     IAVLMGVIADPEPQAVHGHAPGDTAVGGGHNE"
     gene            3876..>4226
                     /locus_tag="AC16A0_04505"
     CDS             3876..>4226
                     /locus_tag="AC16A0_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HIR3104624.1"
                     /translation="MELRPDGRQSETALAIQRGVCRLLRQAGFAVLPEFTLGSGRRAD
                     VIALNPAGLIWIVEIKSSLADFRCDMKWPEYRDYCDRLFFAIPGDFDPEIMPADTGLI
                     IADMFGAEVIREAPE"
BASE COUNT          890 a         1303 c         1348 g          685 t
ORIGIN      
        1 gcagcgatga tggcatcggc aatgaccggc tgcagcgcgc cgagatcgcg attggccatt
       61 gctgccgcct tcttgaccat ggcaagcgac tgcacgaatt caggataatc ccgcagcaga
      121 atcccggtga tcgggaaatt gtccgccgcc cgggccgaat ggatgccgaa gtcggcatcg
      181 tggggaaccg gccactcgcc gattgaatcg cgttccgtgc gggtaagggt catgacagcc
      241 tcccagtgca ttgaatttgg gcactctaca cccgaacggc ccgacaatgt gatgattgcc
      301 taacccattt cccgtcgagt agtgtgaccg gaccgggctg ccgcagtgcc gcccgccata
      361 agaaaaaatc gcccgcaccc aatgaggaga catcatggat cgccgtaatt tcatccgctc
      421 ggccggcgtt gccgccgctg ccgttccagc ccttgccgct cccgccatcg cccagaccat
      481 gcccgagatc aaatggcgca tgacctcgag cttccccaag gcgctcgaca cgatctatgg
      541 cgcagccgag accttcgccc gctacgtggc cgaggccacc gacaaccggt tccagatcca
      601 ggtctttccg gctggcgaga tcgcgcccgg gctcgaggcc gcaaatgaag tgggcaaggg
      661 ctccatcgag atgtgccata cggccagcta ttattactgg ggcaaggacc ccacctatgc
      721 gctggccacc gccgtaccct tcggcctgaa cgcccggcag cagaacgcct ggatgtatta
      781 cggcggcggc gtcgacctga tgaacgagtt ctacgccacg cagaacctca tcggctttcc
      841 ctgcggcaac accggcgccc agatgggcgg ctggttccgc aaggagatca actcggtggc
      901 cgacctgcag ggcgtcaaga tgcgcatcgg cggcttcggc ggcaaggtga tctcaaagat
      961 cggcgtggta ccgcagcaga ttccgggcgg cgagatctat ccggccctcg agaagggcac
     1021 gatcgatgcc gccgagtggg tcggccccta tgacgacgag aagctgggct tctacaaggt
     1081 tgccaagtac tactactatc ccggctggtg ggaaggcggc gccatgcttc acaccatggt
     1141 gaaccttgcc aagtggggtg aattgccgcc ggcctatcag gccatcgtca agtcggccag
     1201 ccaggcggcc aactgcgaca tgatggcaag ctacgatgcc aagaacccga cggcgatcaa
     1261 gagcctcgtg gccaatggcg cggtactgcg gccctatccg caggacgtca tggaggcctg
     1321 cttcaacgcc gccaacgaga cctatgcgga gatcaatgcc agcaacgcgg ccttcaagaa
     1381 gatctatgac tcgatggccg cctatcgccg cgatgcctat ctctggctgc aggtgtcgga
     1441 agcgacctat gacaacttca tgatggcgca gcagcgcaag aaggcgctct gatcttcgcg
     1501 atccgatttc aaggagaagg gcccctcatg gggccctttt cactttcagt cgaaggccgg
     1561 cggtggcggc acgccacctg actctccgcc acccgtttcg atggcgggga tctcgatctc
     1621 gatcgaattc ggatcgacct tcggtccgct gtccggcagt gcgatccatg ggaaggcgac
     1681 ggtgacagct atcatgatgg cctgcaggat gatgaaggac actgcaccgc gatagatctg
     1741 cgttgtcgag atgccggcga tccttccgcc ggtgaccttg tcgatgtagt ccttcaccgg
     1801 cgccaccgag cgcatgtaga acagcgcaaa gccaaagggc ggcgtgagga agctggtctg
     1861 gaggttcacg cccagcagca cgccgaacca gacgagatcg atgccgagct ttgccgcaac
     1921 cggggccagc agcggcacga cgatgaaggc gatctcgaag aagtcgatga agcagccgag
     1981 gataaagacc aggatgttga cggcgatcag gaaacccatg acgccgccgg gcacgctgag
     2041 gaagagctcc tcgacccaga catggccgtt gatgccatag aaggtgagcg agaagatgcg
     2101 gctgccgatc aggatgaaga gcacgaagca ggacagccgg gtggtggcat cgagcgcatc
     2161 gctgagcagc ctgaagctga ggcgccgctt ggcaatggcc atgagcagtg cgccggccgc
     2221 ccccatggcg ccgccctcgg tgggcgtggc gatgcccagg aagatcgttc ccagcacgag
     2281 gaagatcagg cccagcggcg gcaccagcac gatgacgacc tgctggacga gatgggacag
     2341 ccggccgagc ttcagatgct tgtcggccag cgcgaagaga taggtcgcaa cggtggcaat
     2401 ggtcgctgcc cagatgcctg cgccggtttc catctggcgg gcgagcagcc aataggcggc
     2461 aagaccaatc accagggtac cagccagaat ggcgagcaac ggggccggac ttcccttcag
     2521 ggtacgcgcc gccagcggca gcgccggaac cttctgcggc gagacgaccg agacgaagaa
     2581 gacataggca caatagaggc ctgccagcat caggccgggg atcatggcgc cggcatacat
     2641 gtcgcccacc gagcggccga gctggtcggc cagcacgatc agcaccagcg agggcgggat
     2701 gatctgggcc agcgtgcccg aggcggcgat cacgccggtg gccagcggcc ggtcgtaacc
     2761 gtagcgcagc atgatgggca gcgagatcag gcccatggcg atgaccgaag ccgccacgac
     2821 accggtggtg gccgccagca gggcgcccac caggatcacc gcataggcca ggccgccgcg
     2881 gatcggtccg aagagctggc cgatggtgtc gagcagatct tccgccatgc cggacttctc
     2941 gagaatgatg cccatgaagg tgaagaaggg gatggccagc agcgtgtcgt tgcgcatcac
     3001 gtcgagaacc cggccaggca gcgtctgcaa caggggccag tcgagcgcga tctcgttgga
     3061 atagggcgag agctcaaccg caatgacgaa gaagaggaga ccattggcgg ccagcgagaa
     3121 ggccacggga tagccaagca gcaggaacgc gatcagcgac aggaacatga tcggcgcgag
     3181 attgtgggca atgagctcga tcattcgttg tgccctccgc ccacggcggt gtcgccaggc
     3241 gcatggccat gaacggcctg gggctcggga tcggcgatca cgcccatcag cacggcaatc
     3301 cgcttgatca gttcggaggc catctgaagc agcagcagga cgaagcctgc aagcaccagg
     3361 aacttggctg gccagaccga gagtcctccg gcattggtgg acatctcgcc acccgtgtag
     3421 ctgtcccaga agaagggaat cgaaagccag atcatgatgg ccgcgagggg tgccagcatg
     3481 aggatgtggc agactgcgtc gatgatgtcg cgggtccgct tggacagccg gctcgacacg
     3541 acatcgatgc gaacatgggc gttcacctgc agcgtatagg cagcggcgag catgaagacc
     3601 gccccaaaga gcagccattg caattcgagc caggcatttg aacttgaatc gaaggccttg
     3661 cggatgatgg cattgccggc acttactagc actgcggcca ggatcagcca gcgcacattg
     3721 tagccgatga agcgcgtcac ggcgtcgatc atccggctga tggacagcag gattcccatg
     3781 cgtattctcc ccttcgcagc cgtggcccgg cgcctgtatt atggcgtcca cacttacgag
     3841 actcgcctgt cgtcaacaag aggactttcg tcgccgtgga gcttcgcccc gacggacgcc
     3901 aatcggaaac cgccctcgcc atacagcgcg gcgtctgccg cctgctgcgc caggccggat
     3961 ttgccgtttt gccggagttt acccttggct ccggccggcg ggccgatgtc attgccctca
     4021 atccggcagg actgatctgg atcgtcgaga tcaagtcatc gctggccgat ttccgctgcg
     4081 acatgaaatg gcccgagtac cgggactact gcgatcgcct cttcttcgcc attcccggcg
     4141 atttcgaccc cgagatcatg cccgccgata ccggactgat cattgcagac atgttcggcg
     4201 ccgaggtcat ccgcgaggcg ccagaa
//
LOCUS       DBNGVR010000204         2349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3414570, whole genome shotgun sequence.
ACCESSION   DBNGVR010000204 DBNGVR010000000
VERSION     DBNGVR010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3414570"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="AC16A0_04510"
     CDS             complement(<1..159)
                     /locus_tag="AC16A0_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3104625.1"
                     /translation="MTERTPLLELKGVSKRYGGIAALTDVDFKAYAGEIHAVLGENGA
                     GKSTLIKIA"
     gene            complement(231..1358)
                     /locus_tag="AC16A0_04515"
     CDS             complement(231..1358)
                     /locus_tag="AC16A0_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3104626.1"
                     /translation="MVRRFLKVLTGTVAALSFATSAIAGPEVVSGPGVQPECFAPLSA
                     ETKFLKFPKKEGPYRIALANGFVGNTWRIQMIKTAKAYSEQPDVAKDIKELKVVSTGT
                     DVAAQIAAIDNFINSGYDAILTIAVNPTAFTPVIKRANKAGVVLVAFDNTFDSDEVIQ
                     VNENQFQIGVINGTWIKKNVPNGGKILEVRGLPGNSVDRDRHLGIRSVLEAADGPKYE
                     IIEVVGNWDDGTAQKVTADAIAVHKKFDGVTVQGGSTGTVRALMDAGHPFVPVAGEAE
                     NGFRKLCAENADKGLLCSSAGQSPGLVAIAMKTAIEALKGETLPQFVSVPIPLVEHPD
                     FKADVNFFPALTDNFFNSNDFPPCGVNILATDIMGKSEADN"
     gene            complement(1722..>2349)
                     /locus_tag="AC16A0_04520"
     CDS             complement(1722..>2349)
                     /locus_tag="AC16A0_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR3104627.1"
                     /translation="IISSATMSPDDARRLAGRAEALGLHYLDAPISGGAARAAAGELT
                     VMASGTPQAFATARPALEAMAATVYELGDAAGTGAAFKMVNQLLAGVHIAAACEAMTF
                     AAKLGLDLPTVYKVITASAGNSWMFENRVPHILDGDYSPRSAVEIFTKDLGIVSDMGR
                     KEKFPLSIAATALQMFLMTAASGMAKDDDASVARMIARMTGIDLPPAK"
BASE COUNT          411 a          740 c          742 g          456 t
ORIGIN      
        1 ggcgatcttg atgagcgtcg acttgccggc accattctcg cccagaacgg cgtgaatctc
       61 tcctgcatag gccttgaagt cgacatccgt cagcgcggcg atgccgccgt agcgtttgga
      121 gacgcccttg agttcgagaa gcggggtgcg ttcagtcatg gcctgggccg gaaggcagcc
      181 tgacggatgg ccggagccat ccgccaggca ttacctgatc cgcactgctc tcagttgtca
      241 gcctcggact tgcccatgat gtcggtggca aggatgttca cgccgcaggg cgggaaatca
      301 ttcgagttga agaagttgtc cgtcaatgcc gggaagaagt tcacatcagc cttgaagtcc
      361 ggatgctcga ccagcgggat cggcaccgag acgaattgcg gcagcgtctc gcccttcagg
      421 gcttcgattg ccgtcttcat ggcgatggcc acgaggcccg gagactggcc ggccgaggaa
      481 cacagcaagc ccttgtcggc attctcggcg caaagcttgc ggaagccgtt ctcggcctcg
      541 cccgccaccg gcacgaaggg atgcccggca tccatcaggg cacggacggt tccggtcgag
      601 ccgccctgca ccgtcacgcc gtcgaacttc ttgtgcacgg cgatggcgtc ggcggtcacc
      661 ttctgcgctg tgccgtcgtc ccagttgccg accacttcga tgatctcgta cttcggcccg
      721 tcagcggcct ccagcacgct gcggatgccg aggtggcggt cacggtccac cgagttgccc
      781 ggcaggccgc gcacttcgag gatcttgccg ccgttcggca cgttcttctt gatccaggtc
      841 ccgttgatga cgccgatctg gaactggttc tcgttgacct ggatgacttc gtcgctgtcg
      901 aaggtattgt cgaaggcgac cagcaccacg cccgccttgt tggcgcgctt gatgacgggc
      961 gtgaaggccg tcgggttcac cgcgatggtg aggatggcat catagcccga gttgatgaag
     1021 ttatcgatgg cggcgatctg ggccgcgacg tcggtgcccg tcgacacgac cttgagttcc
     1081 ttgatgtcct tggcgacgtc aggctgctcc gaataggcct tggccgtctt gatcatctgg
     1141 atgcgccagg tattgcccac gaagccgttg gccagcgcga tgcgataggg cccttccttc
     1201 ttcgggaatt tcaggaactt cgtctcggcc gaaagcggcg cgaagcattc cggctgaacg
     1261 ccaggtccgg agacgacctc cggaccggcg atggcggaag tcgcaaagct caaggccgca
     1321 actgttccgg taagcacttt caagaaacgt ctgaccacgg cacgtcctcc catctgttgt
     1381 tgttgtcgtc tgcccagcgg aacgttccgc cgctggggtt tgaatcgcgg gggcaagact
     1441 ccgcatatcc agttcttcgc gatttcagag agcgctttcg tcctggctcc gcgacgtccc
     1501 ccacaaaaca aatcacatcc gccgccgagg cgacaaggat cacgaacgat tgctgaaggc
     1561 agtgcgacct gccgtccgat cgcatgtgta ttgccgatca acttatcccg tcacgatgaa
     1621 ctgtcaatgg cagatcaggg acttatctgc tgcggcgcag catcgaaccc agtcacgctt
     1681 cgagactcga gagaccgatg ccgctcctgc gcgcgcgcca ttcacttggc aggcgggaga
     1741 tcgattcctg tcatccgcgc gatcatgcgt gccaccgatg catcatcgtc cttggccatg
     1801 ccggaggccg cagtcatcag gaacatctgc agcgccgtcg cagcgatcga gagcgggaac
     1861 ttctccttgc ggcccatgtc gctgacgatc cccagatcct tggtgaagat ctccaccgcg
     1921 ctgcgggggg aatagtcgcc gtcgagaata tgcggcacgc ggttctcgaa catccagctg
     1981 ttgcccgccg aagcggtgat gaccttgtag acggtgggta gatccaggcc cagctttgcg
     2041 gcgaaggtca tggcctcgca ggccgcggcg atgtgaacgc cggcgagcag ctggttcacc
     2101 atcttgaagg cagcgccggt gccggcagcg tcgcccagtt cgtagaccgt tgcagccatg
     2161 gcctcgagcg ccggccgagc cgttgcgaag gcctgcggcg ttccggacgc catgacggtc
     2221 aactcgccgg ccgcagccct ggccgcgccg ccgctgatgg gcgcgtcgag atagtgaagg
     2281 ccgagcgctt cggcgcgccc cgccagccgc cgcgcatcgt cgggcgacat ggtggccgac
     2341 gagatgatg
//
LOCUS       DBNGVR010000205         6107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1059340, whole genome shotgun sequence.
ACCESSION   DBNGVR010000205 DBNGVR010000000
VERSION     DBNGVR010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1059340"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="AC16A0_04525"
     CDS             <1..1415
                     /locus_tag="AC16A0_04525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3104628.1"
                     /translation="RLERNYRSTPDILGAASGLIAKNESRLGKTLRTDREESEPVTVR
                     SHWDGDEEARTIGDDIESFQRKGDRLNDMAILVRASFQMRSFEDRFITLGVPYRVVGG
                     PRFYERAEIRDALAYLKIIASPDNDLAFERIINTPKRGIGDTSVKRMHEHARNQGISL
                     YRAAEALSLTDELPNKTRTALRTLMESFSRWRQAMDGLPHTELAEIVLDESGYTAMWQ
                     NDKSPEAEGRLENLKELVRSMGEFENLLGFLEHISLVMDVEQSPDDDKVNIMTLHSAK
                     GLEFGTVFLPGWEEGLFPHQRALDEKGRAGLEEERRLAYVGITRAKRRAFISFAQNRR
                     VHNLWQSALPSRFIDELPESHVEVAPMSTSYGGYGLGSYGKSRFEEQSFTNTYATPGW
                     QRAQERWAKGDSARATPRFIEGEMVARETEGGGFDKGERVFHQKFGYGLITSVDGNKL
                     TIDFDKAGEKRVIDSFVERT"
     gene            complement(1395..2798)
                     /locus_tag="AC16A0_04530"
     CDS             complement(1395..2798)
                     /locus_tag="AC16A0_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR3104629.1"
                     /translation="MPSATRLIPLIIASALFMENMDQTIIATSLPAIAADLGTSPIVL
                     KLAFTTYLLSLTVFIPISGWLADRFGARHVFRVAIAVFTLGSVACGLSSSLGELVAAR
                     ALQGLGGAMMVPVGRIILLRAIPKAELVDALAWLTIPALLGPLLGPPVGGFITDSFGW
                     RWIFWMNVPFGIAAILLATWLMPETGVDHVPPLDVRGFLLSGLGLSSLIFGLTVLGRD
                     LLPAYAPPLLMGFGLVMLVLYYLHARRIAAPILDFRLLRIPTFRAGVLGGSLFRIGVG
                     AVPFLLPLMFQLGYGLSAFESGMITCAAAAGAISMKFGAARLIRRFGFRRLLLVNGAL
                     ASVSIAVMGLLTGATPYLLMVALLFVGGFLRSLQFTAMNAMSYSDVDNDGMSYATTFY
                     TVSQQISLSAGVVLGAFVLEAAQWWRSEASLQPADFTIAFFVVAVASLSALFSFRRLA
                     GDAGASVSGKTSRPLDE"
     gene            3038..4846
                     /locus_tag="AC16A0_04535"
     CDS             3038..4846
                     /locus_tag="AC16A0_04535"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104630.1"
                     /translation="MPPPGLLAFAWHYTKPFWPLLAVSMLFSAAIAFLEVYLFAFLGD
                     LVDFLTAANRETFWQDHGTKLIVMGAVVLIVLPLLNFLSESISHQGLRGNYAMRIRWT
                     AHRYVLRQSMDFFTNDFAGRVATKVMQTALGVRDAVMKFTEVIVYVSVYFVGALVLVA
                     TSDLWLMVPMLIWLAGYVAACWYFVPRLGRISAEQADMRSLVTGRVVDSYTNIPTVKL
                     FAHHDREDGYAKEGMTWMLDTVHRSMRLSTLMTSTLQILSGVLIFTMAAISISLWYNN
                     AITTGAIAFAIGLTLRIKAMSQWIIWEVASLFEDVGVIQDGVETIARERSIKDDEDAR
                     PLVVSRGEIRFEDVTFDYGRVAPVRGRTVVDHLNLTIRPGEKVGLVGRSGAGKSTLAN
                     LLLRFYDLQQGRILIDGQDIARTTQDSLRAAIGVVTQDTSLLHRSVIDNIRYGNPDAT
                     MADVEAAARKAHADEFIAHLVDPKGRKGYDAHVGERGVKLSGGQRQRIAIARVLLKNA
                     PILVLDEATSALDSEVEQAIQENLYTLMEGKTVIAVAHRLSTIAAMDRLVIMDKGRIV
                     EEGTHKELLALDGVYASLWRRQSGGFLVAEDLAAAE"
     gene            4911..5570
                     /locus_tag="AC16A0_04540"
     CDS             4911..5570
                     /locus_tag="AC16A0_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaD family glycine zipper/OmpA-like
                     lipoprotein"
                     /protein_id="HIR3104631.1"
                     /translation="MLRKISIILCAALMLAACSTDPYTGEQKISNTAVGAGAGALLGS
                     AAGLVIGKTTSASTRKSVLIGAGIGALAGGGVGVYMDRQEAKLRERLRGSGVSVTRRG
                     DNIILNMPSNITFDTDRADVKPQFFDVLNSVAIVLREFDQTLVDVTGHTDSTGSAAHN
                     LELSERRAGAVAEYLVAQGNNYRRFQVLGMGKSQPIASNATEAGRAENRRVELQIAPL
                     T"
     gene            5578..>6107
                     /locus_tag="AC16A0_04545"
     CDS             5578..>6107
                     /locus_tag="AC16A0_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR3104632.1"
                     /translation="MDHPVLTAIRRASFTPLGWFAPAPDDRVPDSARFVILIGNAGPD
                     MFRRFARERDPDRETLDDWTRDVVSRLAVDVGARAVYPFDTPPQPFLTWALRGGAGHV
                     SPLGLNIHPTYGLWHAYRAALLFPVVFDLPPPSAGRHPCESCAGRPCLTACPVTAFDG
                     TAYDVEACRSHIAMPQG"
BASE COUNT         1123 a         2065 c         1961 g          958 t
ORIGIN      
        1 tccgcctcga gcgcaactac cgctcgacgc cagatattct cggcgccgcc tcgggcctca
       61 tcgccaagaa cgagagtcgg ctgggcaaga cgctccgcac cgaccgcgaa gagagcgagc
      121 ccgtgacggt gcggtcgcac tgggacggcg acgaggaagc ccgcacaatc ggcgacgaca
      181 tcgaaagttt ccagcgcaag ggcgatcgcc tcaacgacat ggcgatcctg gtgcgggcct
      241 ccttccagat gcggagtttc gaggaccgct tcatcacgct gggggtgccc taccgcgtgg
      301 tcggcggccc gcgcttctac gaacgcgccg aaatccgcga cgcgctggcc tatctcaaga
      361 tcatcgcctc gccggacaat gatctcgcct tcgagcgcat catcaatacg cccaagcgcg
      421 gcatcggaga cacttcggtc aagcgcatgc atgagcatgc ccgcaaccag ggcatctcgc
      481 tctaccgtgc ggccgaggcg ctgagcctca ccgatgaact gcccaacaag acccgcacgg
      541 cgctgcgcac gctgatggaa agcttctcgc gctggcgcca ggcgatggat ggactgccgc
      601 acacggaact cgctgaaatc gttctggacg agtccggcta cacggccatg tggcagaacg
      661 acaagagccc ggaagccgag ggccgactcg agaacctcaa ggaactcgtg cgatccatgg
      721 gcgaattcga gaacctgctg ggcttcctcg agcacatctc gctggtcatg gacgtggagc
      781 agtcgcccga cgacgacaag gtcaacatca tgacgctgca ctcggccaag gggctggagt
      841 tcggaacggt cttcctgccc ggatgggagg aaggcctctt cccgcaccag cgcgcgctcg
      901 acgagaaggg acgcgccggc ctcgaagagg agcgccgcct cgcctatgtc ggcatcaccc
      961 gggccaagcg ccgggccttc atttccttcg cgcagaaccg ccgagtccac aatctctggc
     1021 aatcggccct gccctcgcgc ttcatcgacg agttgcccga gtcccatgtc gaggtggcgc
     1081 ccatgtccac cagctatggc ggctacggcc ttggctccta cggcaagagc cgcttcgagg
     1141 agcagagctt caccaacacc tatgccacgc ccggctggca gcgcgcccag gagcgctggg
     1201 ccaagggcga cagcgcccgc gcgacgccgc gcttcatcga gggcgagatg gtggcgcgcg
     1261 aaaccgaggg cggcgggttc gacaagggcg agcgcgtctt ccaccagaag ttcggctatg
     1321 gcttgatcac cagcgtcgac ggcaacaagc tgaccatcga tttcgacaag gccggcgaga
     1381 agcgcgtgat cgactcattc gtcgagcgga cgtgacgtct tgcccgacac gctggcgccg
     1441 gcgtcgcccg cgaggcggcg gaagctgaac agggccgaga gcgacgccac tgccaccacg
     1501 aagaaggcga tggtgaaatc cgccggctgc aggctggcct cgctgcgcca ccactgcgcg
     1561 gcctccagca cgaaggcgcc cagcaccacg ccggccgaca gcgagatctg ctgcgacacg
     1621 gtatagaagg tggtggcata gctcatgccg tcattgtcga cgtcggaata ggacatggcg
     1681 ttcatcgccg tgaactgcag cgagcgcagg aagccgccga cgaagagcaa ggccaccatc
     1741 agcagatagg gcgtggcacc ggtcaggagc cccatgacgg cgatcgagac ggacgccagc
     1801 gcgccgttca ccagcaacag gcggcggaag ccgaagcgcc ggatcaggcg ggccgcgccg
     1861 aacttcatcg agatggcgcc ggccgcggcg gcgcacgtga tcatgcccga ctcgaaggcg
     1921 ctgaggccat agcccagctg gaacatcagc ggcaggagga agggaaccgc gccgaccccg
     1981 atacggaaca ggctgccacc caggacgccc gcgcgaaagg tggggatgcg cagcaggcgg
     2041 aaatcgagga tgggtgctgc gatgcggcgg gcgtgcaggt agtagaggac gagcatcacc
     2101 aggccgaagc ccatgagcag cggcggcgca taggccggca gcaggtcgcg gcccagcacg
     2161 gtcaggccga agatcagcga agacaggccg agccccgaca agagaaagcc cctgacgtcg
     2221 agcggcggca cgtggtcgac tccggtttcc ggcatgagcc aggtggcgag caggatggcg
     2281 gcaataccga aaggcacgtt catccagaag atccagcgcc agccgaagct gtcggtgatg
     2341 aacccgccga ccggcggccc gagcagcggc cccaggagag ccggtatcgt gagccaggcc
     2401 agcgcgtcga caagctcggc cttgggaatg gcccggagca ggatgatgcg cccgaccggc
     2461 accatcatgg cgccgcccag gccctgcagg gcgcgcgccg ccaccagttc gcccagcgag
     2521 gacgagaggc cgcaggccac cgacccaagt gtgaagacgg caatggccac ccggaacacg
     2581 tgccgcgcgc cgaatcggtc ggccagccag ccgctgatcg ggatgaagac cgtgagactc
     2641 agcaaatagg tggtgaaggc cagtttcagc acgatggggc tggtgccgag atcggcagcg
     2701 atcgccggca gcgacgtggc gatgatcgtc tggtccatgt tctccatgaa cagggccgag
     2761 gcgatgatca gtggtatgag acgcgtggcg gacggcatgt ccggtaccta gcatgcaggc
     2821 atgcagaggc tcatgcattt tccggggact tgccgtgccg gaaacactgt aatcctgtgc
     2881 cctgccgcca ctttgcggcc ccgattcgcg ggtctataag gccctcgatc cgtatcgaat
     2941 tcggcaccca cggcggtgcc tggagttatc cgtgttcaac cgcttcttcc actggcttga
     3001 aagccgggtt gatccctttc cgcctgaccg gccgcagatg ccgccgccgg gcctgctggc
     3061 ctttgcctgg cactatacca agcccttctg gccgctgctg gcggtgtcga tgctgttttc
     3121 ggccgccatc gccttcctcg aagtctatct cttcgccttc ctcggcgacc tcgtcgactt
     3181 tctcaccgcg gcgaaccgcg aaaccttctg gcaggaccat ggcaccaagc tcatcgtgat
     3241 gggcgcggtg gtgctcatcg ttctgccgct gctgaacttc ctatccgaat ccatttcgca
     3301 tcagggcctg cggggcaatt atgccatgcg catccgctgg accgcgcacc gctatgtctt
     3361 gcgccagagc atggacttct tcaccaacga cttcgccggg cgcgtcgcca ccaaggtgat
     3421 gcagaccgca ctgggcgtgc gcgatgcggt gatgaagttc accgaggtca tcgtctatgt
     3481 cagcgtctac ttcgtcggcg ccctggtgct ggtggccacc agcgatctct ggctcatggt
     3541 gcccatgctg atctggctgg ccggctatgt ggccgcctgc tggtacttcg tgccgcggct
     3601 cggccgcatc tcggccgaac aggccgacat gcgttcgctg gtcaccggcc gggtggtgga
     3661 cagctacacc aatatcccca ccgtcaagct cttcgcccat cacgaccgcg aggacggcta
     3721 tgccaaggaa ggcatgacct ggatgctgga cacggtccac cgctccatgc gcctgtcgac
     3781 gctcatgaca tcgacgctgc agatcctctc tggcgtgctg atcttcacca tggccgccat
     3841 ctcgatctcg ctgtggtaca acaatgccat caccaccggg gcaatcgcct ttgccatcgg
     3901 cctcacgctg cgcatcaagg ccatgtcgca gtggatcatc tgggaagtgg ccagcctctt
     3961 cgaggacgtc ggcgtcatcc aggacggcgt cgagaccatt gcgcgcgagc gcagcatcaa
     4021 ggatgacgag gacgcccgcc ccctggtcgt cagccggggc gaaatccgct tcgaggacgt
     4081 gaccttcgat tatggccgcg tcgcaccggt gcgcggccgc accgtggtcg atcacctgaa
     4141 tctcaccatc aggccgggcg agaaggtggg gctcgtcggg cgttccggcg cgggcaagtc
     4201 gacgctggcc aatctgctgc tccgcttcta cgacctgcag caaggccgca tcctgatcga
     4261 cggacaggat atcgccagga cgacgcagga ttcgctgcgc gccgcgatcg gcgtggtgac
     4321 gcaggacacc tcgctgctgc accgttccgt gatcgacaac atccgctacg gcaatcccga
     4381 cgcgaccatg gcagacgtcg aggcggcagc tcgcaaggcc catgccgacg agttcatcgc
     4441 gcatcttgtc gatccgaagg ggcggaaggg ttacgacgcg catgtcggcg agcgcggtgt
     4501 gaagctctca ggcgggcagc gccagcgcat cgccatcgcg cgcgtgctgc tcaagaacgc
     4561 gccgatcctg gtgctcgacg aggccaccag cgcgctcgac tccgaagtgg agcaggccat
     4621 tcaggaaaat ctctacacgc tgatggaagg aaagaccgtc atcgcggtgg cgcatcggct
     4681 ctccaccatc gcggccatgg accggctggt gatcatggac aagggtcgca tcgtcgaaga
     4741 aggcacccac aaggaactcc ttgcgctaga tggagtttat gcctcgttgt ggcgccgcca
     4801 gtccggcggc ttccttgtgg ccgaagacct ggctgccgca gaataaccca tcgttaatca
     4861 tattgcggcc acatggggcg ttgatgaacc tgcgaaaacc gggggaaacc atgctgcgta
     4921 aaatctcgat catcctgtgc gccgccctga tgctggccgc ctgctccacc gatccctata
     4981 cgggcgagca gaagatctcg aacacggccg tcggtgccgg tgccggcgcg ctgcttggca
     5041 gtgccgcagg cctcgtgatc ggcaagacca ccagcgcctc cacccgcaag tccgtgctga
     5101 tcggcgccgg catcggcgcg ctggccggcg gcggcgtggg cgtctacatg gaccgccagg
     5161 aagccaagct gcgcgaacgc ctgcgcggca gcggcgtctc ggtcacgcgg cgcggcgaca
     5221 acatcattct caacatgccg tccaacatca ccttcgacac cgaccgcgcc gacgtgaagc
     5281 cgcagttctt cgacgtgctg aactcggtgg cgatcgtgct gcgcgaattc gaccagacgc
     5341 tggtcgacgt caccggccac accgactcga cgggcagtgc cgcgcataac ctcgaactgt
     5401 cagagcgccg tgccggtgcc gtggcggagt atctggtggc gcagggaaac aactaccggc
     5461 gcttccaggt cctgggcatg ggcaagagcc agccgattgc cagcaatgcc accgaggccg
     5521 ggcgcgccga gaaccgccgc gtcgagctcc agatcgcccc gctgacctga gcggatcatg
     5581 gaccacccgg tcctgaccgc catccgccgc gccagcttca cgccgctggg ctggttcgcg
     5641 ccggcgccgg acgaccgggt gccggacagc gcccggttcg tgatcctgat cggcaatgcg
     5701 ggacccgaca tgttccgccg cttcgcccgc gaacgcgatc ccgaccgcga gacgctggac
     5761 gactggaccc gcgacgtggt gtcccgcctg gcggtcgacg tcggcgcccg ggccgtctat
     5821 cccttcgaca ccccgccgca gcccttcctg acctgggccc tgcgcggcgg cgccgggcat
     5881 gtctcaccgc tgggcctcaa catccatccg acctacgggc tctggcacgc ctaccgtgct
     5941 gcactgctct tcccggtcgt cttcgacctg ccgccgccat cggcgggacg ccacccttgc
     6001 gagtcctgtg ccggccgccc ctgcctgacc gcctgccccg ttacggcctt cgacggcacg
     6061 gcctatgacg tggaggcctg ccggtctcac atcgccatgc cgcaagg
//
LOCUS       DBNGVR010000206         3229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1452337, whole genome shotgun sequence.
ACCESSION   DBNGVR010000206 DBNGVR010000000
VERSION     DBNGVR010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1452337"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16A0_04550"
     CDS             <1..615
                     /locus_tag="AC16A0_04550"
                     /inference="COORDINATES: protein motif:HMM:NF025912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3104633.1"
                     /translation="QPVDGFMGALPESQLKQFVDRLGGQGGMAEEIEAAVAEARLMME
                     QGDLQTAAQIFASVLQADREHAGALAGLARCQIAAGDLENARATLALVPPSKASDPDV
                     LSATAALDLALNPVDLSEAATLRAAIDRNPDDFQARLDLAVLLNGAGERTEATEQLLY
                     VIRKKRDWNEEAARKQLVKFFEAWGPKDEFTLAGRRKLSSLLFS"
     gene            627..1295
                     /locus_tag="AC16A0_04555"
     CDS             627..1295
                     /locus_tag="AC16A0_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3104634.1"
                     /translation="MAFLDRYRTPADLPQRLPVFPLSGALLLPRAELPLNIFEPRYLE
                     MMNDAMAGDRLIGMIQPAPLSTGDERPDLLKVGCAGRITAYSETPDNRLLVTLTGVSR
                     FAIREELDVDTLYRQVVADFHPYAVDLVSDLGAGDVNRPALLKAFRDYLNANDMKADW
                     DQVDAASTEVLVNTLSLLAPYPPEEKQALLEAPDLKTRADVLVALTEMALNRMGAGGK
                     TRLQ"
     gene            1295..1483
                     /locus_tag="AC16A0_04560"
     CDS             1295..1483
                     /locus_tag="AC16A0_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR3104635.1"
                     /translation="MPDTWRTDSRLLQMLVCPLTKMPLSYDAERQELVSKAAGLAYPI
                     RDGIPIMLPEEARDINRE"
     gene            1476..1829
                     /locus_tag="AC16A0_04565"
     CDS             1476..1829
                     /locus_tag="AC16A0_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR3104636.1"
                     /translation="MNDPWPEELRVSDKGRVLHVTFDSGERHALDAEYLRVESPSAEV
                     KGHGPGQEQLVWGKRDVAIAKLEPVGSYAVKITFSDGHNTGLFTWPYLLKLGREREAI
                     WSAYVDKLQAAGLRR"
     gene            1934..>3229
                     /locus_tag="AC16A0_04570"
     CDS             1934..>3229
                     /locus_tag="AC16A0_04570"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltF family protein"
                     /protein_id="HIR3104637.1"
                     /translation="MPRPQFTIIIAIVSLLSFLILAKAPAGASDVHDLEGFKPWTGDL
                     EGMKKRRVVRILVPYSKTIYFVDRGRPYGTAVELGQALEKWLNEGKTRQIDMTRVAFV
                     AKPRDQLLPALVQGLGDIAAGNLTITPERRRTVDFTAPFVTDVREVLVTGPDSPPVTS
                     IADLGGKEVFLRPSSSYREHVEAANLRLRAEQRPAIAITGIDEHLEDEDLLEMVNAGL
                     LPWAIVDEHKAGIWAKVLPNLTIRPEIAFSESGEIAWAIRKDSPQLKAELDAFIEKHR
                     IGTTFGNVLRKRYYRDDKMLRRANASAEQAKFREFLDLFRKHGEAYSFDYLLLAAQGY
                     QESQLDQKRRSPRGAVGVMQLMPATAADKAVGVRGIDKSADRNIEARLLRPAQVGVAR
                     LCVLRLQDDRLRRGRSRPACHPGERRAGRCARHDRAPGPR"
BASE COUNT          590 a         1103 c         1036 g          500 t
ORIGIN      
        1 cagccagtcg acggcttcat gggcgcgctg cccgagagcc agctcaagca gttcgtcgac
       61 cgtctcggcg gccagggcgg catggccgag gaaatcgagg ccgcggtggc cgaggcgcgc
      121 ctgatgatgg aacagggcga cctgcagacc gccgcgcaga tcttcgcgtc cgtgctgcag
      181 gccgaccgcg aacatgccgg cgcgcttgcc ggcctcgcgc gctgccagat tgcggcgggc
      241 gatctcgaaa atgccagggc cacgctggcg ctggtgccgc catccaaggc ctctgatccc
      301 gacgtgctga gcgccaccgc cgccctcgac ctcgcgctga acccggtgga cctgtccgag
      361 gccgcaaccc tccgggcggc aatcgacagg aaccccgacg atttccaggc gcggctcgac
      421 ctcgccgtgc tgctcaatgg cgcgggcgaa cgcaccgagg ccaccgagca gctgctttac
      481 gtcatccgca agaagcgcga ctggaacgag gaggccgcgc gcaagcagct ggtgaaattc
      541 ttcgaggcct gggggccgaa ggacgaattc accctcgccg gccgccgcaa gttgtcctcc
      601 ctcctgttct cctgaggcga tcccgcatgg cctttctcga ccgctaccgg acccccgccg
      661 acctgccgca gcgcctgccg gtcttcccgc tgtcaggcgc gctgctgctg ccgcgggccg
      721 agctgccgct caacatcttc gaaccgcgct acctcgagat gatgaacgac gccatggccg
      781 gcgaccggct gatcggcatg atccagcctg ctccgctctc caccggggac gagcggccgg
      841 acctgctgaa ggtgggctgc gccggccgca tcaccgccta ctccgagacg ccggacaacc
      901 ggctgctcgt cacgctcacc ggcgtgtcgc gcttcgccat ccgcgaggag ctcgatgtcg
      961 acacgctcta tcgccaggtg gtggcggact tccatcctta cgccgtcgat ctcgtctccg
     1021 acctcggcgc cggcgacgtg aaccgccccg ccctgctcaa ggccttccgc gactatctca
     1081 atgccaatga catgaaggcc gactgggacc aggtcgatgc cgcctcgacc gaggtgctgg
     1141 tcaataccct gtcgctgctg gcgccctatc cgccggaaga aaagcaggcc ctgctggaag
     1201 cgcccgacct caagacccgg gccgacgtgc tggtagccct gaccgagatg gcgctgaacc
     1261 gcatgggcgc gggcggcaag acgcggctgc agtgatgccg gacacctggc gcaccgattc
     1321 ccgcctgctg cagatgctgg tctgcccgct gaccaagatg ccgctgtcct atgacgccga
     1381 acggcaggag ctggtctcga aggcggccgg cctcgcctat cccatccgcg atggcatacc
     1441 catcatgctt cccgaagagg cgcgggacat caaccgtgaa tgatccgtgg ccggaggaat
     1501 tgcgcgtttc cgacaagggg cgcgtgctcc acgtgacctt cgacagcggc gagcgccatg
     1561 cgcttgacgc cgagtatctc cgggtggaaa gccccagcgc cgaagtaaag gggcatggcc
     1621 ccggccagga acagctggtc tggggcaagc gtgacgtcgc catcgcgaag ctcgaaccgg
     1681 tcggcagcta tgcggtgaag atcaccttca gcgacggcca caacaccggg ctcttcacct
     1741 ggccctacct gttgaagctg gggcgcgaac gcgaggcgat ctggagcgcc tacgtcgaca
     1801 agctccaggc ggcgggcctt cggcgatagg acctggtgga ttcggaactg catcgcggaa
     1861 atggacactc gccacacgtt ggggcaaaag gccatttgac gacaccccga gacctagccg
     1921 acccggattg accatgccca gaccgcagtt caccatcatc atcgcaatcg tcagcctgct
     1981 gagctttctg atccttgcga aagcgcctgc cggtgcaagc gacgtgcatg acctcgaggg
     2041 attcaagccg tggaccggcg acctcgaggg catgaagaag cgccgcgtgg tgcgcatcct
     2101 ggtgccctac agcaagacca tctatttcgt cgacaggggg cgcccgtacg gcacggcggt
     2161 ggagcttggc caggcgctcg agaaatggct caacgagggc aagacgcggc agatcgacat
     2221 gacgcgcgtg gccttcgtcg ccaagccgcg cgaccagctg ctcccggccc tggtgcaggg
     2281 actgggcgac attgcggccg gaaacctgac gatcacgccg gagcggcgca ggaccgtcga
     2341 cttcaccgca cctttcgtca ccgatgtgag ggaggtgctg gtgacaggcc ccgactcgcc
     2401 gcccgtgacg tcgatcgccg accttggcgg caaagaggtc tttctcaggc cgagcagcag
     2461 ctatcgcgag catgtggagg cggcaaatct ccggcttcgc gctgagcaac gccccgcgat
     2521 cgcgatcacc ggcatcgacg agcatctcga agacgaggat ctgctcgaga tggtcaatgc
     2581 cggactcctg ccctgggcga tcgtcgacga acacaaggcc ggaatctggg ccaaggtcct
     2641 gcccaatctg acgatccggc ccgagatcgc cttctccgaa agcggcgaga tcgcctgggc
     2701 catccgcaag gacagccccc agctgaaggc cgaactcgac gccttcatcg agaagcacag
     2761 gatcggcacc actttcggaa acgtgctgcg caagcgctac taccgcgacg acaagatgct
     2821 gcggcgtgcc aatgcctccg ccgagcaggc caagttccgg gaattcctcg acctgttccg
     2881 gaaacacggc gaggcctatt cattcgacta tctgctgctg gcggcacagg ggtatcagga
     2941 gtcgcagctt gaccagaagc ggcgaagtcc gcgcggcgcc gtcggtgtca tgcagctgat
     3001 gccggcgacg gcggccgaca aggcggtcgg cgtcaggggc atcgacaaga gcgccgaccg
     3061 caacatcgag gctcgacttc tacgaccggc tcaagtcggt gtcgcgcggc tatgcgtcct
     3121 tcgactacaa gatgatcggc tacgaagagg gcgatctcgt ccggcttgcc atcctggtga
     3181 acgccgagcc ggtcgatgcg ctcgccatga tcgtgcaccg ggcccgcgc
//
LOCUS       DBNGVR010000207         1607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2826193, whole genome shotgun sequence.
ACCESSION   DBNGVR010000207 DBNGVR010000000
VERSION     DBNGVR010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2826193"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /gene="argH"
                     /locus_tag="AC16A0_04575"
     CDS             complement(<1..1382)
                     /gene="argH"
                     /locus_tag="AC16A0_04575"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3104638.1"
                     /translation="MTNKMWGGRFRSGPDAIMEEINASIGFDQRLFAQDIAGSKAHAA
                     MLAAQGIISKTDAAGIRKGLDAIAREIEDGTFTFSRALEDIHLNVESRLKELIGPAAG
                     RLHTGRSRNDQVALDFRLYVRDTIDHLELQLKDLQLALAEKAEAHAATIMPGFTHLQV
                     AQPVTFGHHCLAYVEMLARDRSRLADARRRMNENPLGAAALAGTSFPIDRHATAKALG
                     FDRPTRNSLDTVADRDFVLETLSACAICATHLSRLAEEIVIWSTSQFRFVKLSDKFTT
                     GSSIMPQKRNPDAAELVRAKPGRIIGALTALLTVMKGLPLTYSKDMQEDKEPAFDAFD
                     NMSLALAATAGMVRDLTPNAEEMRKAAAAGFSTATDLADWLVRTLKMPFREAHHVTGS
                     LVALAETEGCDLPELPLKLMQSVEPRITRDVYKVLTVENSVASRTSYGGTAPKNVRKE
                     ARRWLKLLKT"
     gene            1418..>1607
                     /locus_tag="AC16A0_04580"
     CDS             1418..>1607
                     /locus_tag="AC16A0_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104639.1"
                     /translation="MSKAKAPGPLGQIAAGLAVIAAITGIYLFQAEGRKAGPESQAAS
                     PSVPGPSGISKDLATGSLA"
BASE COUNT          257 a          517 c          539 g          294 t
ORIGIN      
        1 caggttttca gcagcttgag ccagcgccgc gcttccttgc gcacattttt cggtgcggtg
       61 ccgccgtagc tggtgcgcga ggccaccgag ttttccactg tcagcacctt gtagacgtcc
      121 cgcgtgatcc gcggttctac cgactgcatc agcttcagcg gcagctcagg caggtcgcag
      181 ccctcggtct ccgccagcgc caccaggctt ccggtcacgt gatgggcctc gcggaacggc
      241 atcttcagtg tgcggacgag ccagtcggcg aggtcggtcg ccgtcgagaa gccggccgcc
      301 gccgccttcc gcatctcctc ggcgttgggc gtcaggtcgc gcaccatgcc ggcggtcgcc
      361 gcaagggcca gcgacatgtt gtcgaatgca tcgaaggccg gttccttgtc ttcctgcatg
      421 tccttcgaat aggtaagcgg caggcccttc atcacggtca gcagcgcggt cagcgcgccg
      481 atgatgcgcc cgggcttggc gcggaccagt tcggccgcat cgggattgcg cttctgcggc
      541 atgatcgatg agccggtcgt gaatttgtcg gagagcttca cgaagcggaa ttgcgaggtc
      601 gaccagatga cgatctcttc ggccagccgc gacaggtgcg tggcgcagat ggcgcaggcc
      661 gaaagcgtct ccagcacgaa gtcgcgatcg gccacggtat cgagcgaatt gcgggtcggc
      721 cggtcgaagc ccagcgcctt ggccgtcgcg tggcggtcga tggggaaaga ggtgccggcc
      781 agcgccgcgg cgcccagagg gttctcgttc atgcgcctgc gggcatcggc gagccgtgag
      841 cgatcccgcg ccagcatttc cacgtaagca aggcagtggt ggccgaaggt gaccggctga
      901 gccacctgca gatgcgtgaa gcccggcatg atggtggcgg catgcgcctc ggccttttcg
      961 gccagggcca gctggaggtc cttcaattga agttcgagat gatcgatcgt gtcgcgcaca
     1021 tagagtcgga agtcgagcgc cacctggtcg ttgcgcgagc gccctgtgtg caaccgtccg
     1081 gccgccgggc cgatcagctc cttgaggcgc gattcgacgt tcaggtgaat gtcctccagg
     1141 gcgcgcgaga aggtgaacgt accgtcctcg atctcgcgtg cgatggcatc gaggcccttg
     1201 cggatcccgg cggcgtcggt cttgctgatg atcccctgcg ccgccagcat cgccgcatgg
     1261 gccttggagc cggcgatgtc ctgtgcgaag aggcgctggt cgaagccgat cgaggcgttg
     1321 atttcctcca tgatcgcgtc cggcccggag cggaatcggc cgccccacat cttgttggtc
     1381 atcgaatttc cttcttcaca gccgaaaagc gcagatcatg agcaaagcga aagcacctgg
     1441 acccctggga cagatcgcgg cgggcctcgc cgtcatcgcc gcaatcacgg gcatttactt
     1501 gtttcaggcc gaggggcgca aggccggacc ggagagtcag gcggcctccc catccgtccc
     1561 cggcccgtcc ggcatctcga aggacctcgc caccggcagc cttgccg
//
LOCUS       DBNGVR010000208         3078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4988688, whole genome shotgun sequence.
ACCESSION   DBNGVR010000208 DBNGVR010000000
VERSION     DBNGVR010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4988688"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC16A0_04585"
     CDS             complement(<1..603)
                     /locus_tag="AC16A0_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enolase-like domain-containing protein"
                     /protein_id="HIR3104640.1"
                     /translation="MKIIDIEVIELRVPGWTGETFDGSYDNCLVLVHTDEGMTGIAEV
                     ASVPAVIRAIVDAPRSHTHAMGLKQVLLGQDPSDVEGLWDRMYDLTSYYGRRGVVIHA
                     ISAVDIALWDLRGKMQGKSLGRLLGAPQRDRVLAYGTVYPLGETPDEVRKTIDRGLKL
                     GLKAIKIVADPFWRDDLAKTEGLIRAARAHVGPDIRLMVDA"
     gene            676..1512
                     /locus_tag="AC16A0_04590"
     CDS             676..1512
                     /locus_tag="AC16A0_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3104641.1"
                     /translation="MSVSQPIALKNMRKASRPPVIEDESTGSRSLRRALDILDLMLQR
                     GGPLTVAQIIEALSIPKSTAYELVRTLTEANCIEPSGKGSGLYLGRKLYEFGMAYHGH
                     VDLLRDGSIVAEELRNETGETVQLSVLENDQMMVLLKEEGVRPLRIISNVGSRVPINW
                     AAAGRLLVSDLDDKALTRLLAATVRQSPSGKATMDIKKIVAQVRRYRRQGYATELNEA
                     NEHAGCIAAPIIDAAGHCIAALSVVVPEQRLARPNREHLIVSVIKAAEKLSRRIGGPR
                     AA"
     gene            complement(1509..2636)
                     /locus_tag="AC16A0_04595"
     CDS             complement(1509..2636)
                     /locus_tag="AC16A0_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3104642.1"
                     /translation="MRPVKLRGITWDHRRAIDPLINTAAMFREQHPDIEIAWSTRPLS
                     GFEFTPVAELAQSFDLIILDHPFMGAVAASKCLLPLDEDTGADSLFVGPSLATYRLDD
                     HVWALPVDAACQVAVSRPDLMELLDMEPPRNWDGLLRLGRRAREKGQWLAIGLKGVHS
                     LMTFFTLMANLGAPCASDRRHDFADRGMARQALGLMRALLEYCPPQVFDWNSIALHDA
                     MVREDDLVFCPAVYCYATYAEADQRRPLRFHDLPGPAGHAGSTIGGTGLAVSAATTHR
                     EAALAYVRFAATAEAQRAFASHHGQPAHAAVWDDPTVNQSFGGCYAQTRATMEACWIR
                     PRYDGYLAFQEAGGPLVESHLRGDIGADRLLDTLAQLHAGT"
     gene            2765..>3078
                     /locus_tag="AC16A0_04600"
     CDS             2765..>3078
                     /locus_tag="AC16A0_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104643.1"
                     /translation="MKSVKLLYGAAIAAIALTLSAPASLAAAGDECVKILGYEWSGEK
                     QSMDPADMHSGDDAYHTFAVYNRLVDIDDNFNVLPELATEWKVSDDGLTWTFKLREGV
                     KF"
BASE COUNT          584 a          977 c         1004 g          513 t
ORIGIN      
        1 ggcatccacc atgagccgga tgtcggggcc gacatgcgcg cgcgccgccc ggatgaggcc
       61 ttccgtcttg gcgagatcgt cccgccagaa ggggtcggcc acgatcttga tggccttgag
      121 gccgagcttc agcccccggt cgatggtctt gcggacctcg tcgggcgtct cgcccagggg
      181 atacacggtt ccgtaagcca ggacacggtc gcgctgcggc gcgccgagga gccggccaag
      241 gctcttgccc tgcatctttc cgcgcagatc ccacaacgcg atatccacag cggaaatggc
      301 atgaattact acgccgcgcc ggccgtagta actggtgaga tcatacatcc ggtcccacaa
      361 gccctcgaca tcgcttgggt cctggccgag cagaacctgc ttcagtccca tggcatgggt
      421 gtgggaccgc ggcgcatcga cgatggcgcg gatcaccgcg ggcacgctgg cgacttcggc
      481 aatccccgtc atgccttcgt cggtgtggac gaggacgagg caattgtcgt atgagccgtc
      541 gaacgtctcc ccggtccagc cgggaacgcg cagttcgatg acctcgatat cgatgatttt
      601 catgttccgg ttccatttcg ccgaattcca caaataccag ataccggaca gtagtccaca
      661 ggatgggaaa cccaaatgag tgtctcgcag cccattgccc tgaagaacat gcgcaaggcg
      721 agccggccgc cggtcatcga agacgagtcg accggctccc gcagcctccg tcgcgcgctc
      781 gacattctcg atctcatgct gcagcgcggc ggaccattga cggtggcgca gatcatcgaa
      841 gcgctctcca tcccgaagtc gaccgcttac gagctggtcc ggaccctgac ggaagccaat
      901 tgcatcgagc cgtccggcaa gggcagcggc ctctacctgg gccgcaagct ctacgagttc
      961 ggcatggctt accacggcca tgtcgacctg ctgcgcgacg gcagcatcgt ggccgaggaa
     1021 ctgcgcaacg agacgggcga gacggtccag ctctcggtgc tggagaacga ccagatgatg
     1081 gtgctgctca aggaagaggg cgtgcgaccc ttgcgcatca tcagcaatgt cggctcgcgc
     1141 gtgcccatca actgggcggc ggccgggcgg ctgctggtct ccgatcttga cgacaaggcg
     1201 ctgacaaggc tgcttgcagc aaccgtacgc cagtcgcctt cgggcaaggc gacgatggac
     1261 atcaagaaga tcgtcgccca ggtccggcgc taccgccgcc agggctacgc gaccgagctg
     1321 aacgaggcca acgaacatgc cggctgcatt gccgccccga tcatcgacgc agcgggccac
     1381 tgcatcgcgg cgctgagcgt ggtggtgccc gagcagcggc tggcgaggcc caaccgcgaa
     1441 catctcatcg tcagcgtcat caaggcggcc gaaaaactgt cgcgccggat cggcggccca
     1501 cgcgccgctt aagtgccggc gtggagttgc gccagggtat cgaggagccg atccgcgccg
     1561 atgtcgccgc gcagatgtga ctcgacgaga ggaccgcctg cctcctgaaa cgcaagatag
     1621 ccatcatagc gcgggcggat ccagcaggcc tccatggtgg cgcgggtctg cgcatagcag
     1681 ccgccaaagc tctggttgac cgtggggtca tcccagacgg ccgcgtgggc cggttgcccg
     1741 tgatgggacg cgaaggcgcg ctgagcctcc gccgtggcgg caaagcggac ataggccagc
     1801 gccgcctcgc ggtgggttgt tgcagcggaa accgcaagcc ccgtcccgcc gatggtcgag
     1861 ccagcatgcc ccgccgggcc gggcaggtca tgaaaccgca gcggccggcg ctggtcggcc
     1921 tccgcatagg tcgcgtagca atagaccgcg gggcagaaga cgagatcgtc ctcgcgcacc
     1981 atggcgtcat gcagcgcgat gctgttccag tcgaagacct gcggcgggca gtattccagc
     2041 agcgcgcgca tcaggccgag ggcctgccgg gccatgccgc ggtcggcgaa gtcatggcgg
     2101 cgatccgagg cgcagggcgc gccgagattg gccataaggg tgaagaaggt catcaggctg
     2161 tgtacgccct tgagtccgat ggcaagccat tgcccttttt cccgggcgcg ccgtcccagc
     2221 ctcaggaggc cgtcccagtt gcggggcggt tccatgtcca gcaactccat caggtcggga
     2281 cgggacaccg ccacctggca ggcggcatcg acgggcaggg cccagacatg atcgtccagg
     2341 cgataggttg ccagcgaagg gccgacgaac agcgagtccg cgccggtgtc ctcgtccagc
     2401 ggcaagaggc acttcgacgc ggcgacggcc cccatgaagg ggtggtcgag aatgatgaga
     2461 tcgaagcttt gcgccagctc cgcgacaggc gtgaactcga atccgctgag cggccgcgtc
     2521 gaccatgcga tctcgatgtc cggatgctgc tctcggaaca tcgcggccgt gttgatcagc
     2581 ggatcgatcg cgcgccgatg gtcccacgtg atgccacgca atttgactgg tctcatcaac
     2641 gcctccggta tcttgactcg cagtctatcg cagctaatgt gccagtctgg caagacgtac
     2701 agttgtccgc catttcggaa ttgcaagtgc ctttaaacag ataatgtctg ggaggacgta
     2761 tccaatgaag tcggtcaagt tgttgtacgg cgccgccatc gcggcgatcg cattgacgtt
     2821 gtcggcaccg gccagccttg ccgctgcggg tgacgagtgc gtgaagatac tgggttacga
     2881 gtggagcggc gagaagcagt cgatggatcc cgccgacatg cactcgggcg acgatgccta
     2941 tcacacattt gcggtctaca accgcctcgt cgatatcgac gacaacttca acgttctgcc
     3001 ggaactggcc acagaatgga aagtctccga cgacggactg acctggacct tcaagctgcg
     3061 cgagggcgtg aagttcca
//
LOCUS       DBNGVR010000209         4679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2238537, whole genome shotgun sequence.
ACCESSION   DBNGVR010000209 DBNGVR010000000
VERSION     DBNGVR010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2238537"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /gene="puhA"
                     /locus_tag="AC16A0_04605"
     CDS             <1..640
                     /gene="puhA"
                     /locus_tag="AC16A0_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030077 - plasma membrane
                     light-harvesting complex [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="photosynthetic reaction center subunit H"
                     /protein_id="HIR3104644.1"
                     /translation="AHITVQGFPAIPSPKTFKLADGSTVMAPRAEPQDSRPVKATPVG
                     GWLGAPLEPDGNPMLDGVGPAAYAERADIPDATIHGTPKLLPLRAADGFYIDTHDPDP
                     RGMPVIGADGRQGGIVRDVWLDRAEYIIRYLEVEVSDKNGKRNVLLPTTMSRVSGSRN
                     QVEVKSILGKQFANVPAHRKPDVVTRLEEDRICAYYASGHLYATPERAEPLI"
     gene            637..1278
                     /gene="puhB"
                     /locus_tag="AC16A0_04610"
     CDS             637..1278
                     /gene="puhB"
                     /locus_tag="AC16A0_04610"
                     /inference="COORDINATES: protein motif:HMM:NF040894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex putative assembly protein
                     PuhB"
                     /protein_id="HIR3104645.1"
                     /translation="MSDDFDFEPVRGLPEMLPAGERLLWQGAPRWQDLAVHAFHARKV
                     IWYFLAIAIVQAVFRLADGSSLSDASWPFVWLLPMGGIAAAILTFLAYVSSRTTVYSM
                     TTKRLVMRIGIALPVTLNLPFRLVDSASLRLYANGSGEIPLKLAASERVAYLLLWPHA
                     RPLHFRNPQPCLRAIPNADQVAKLLATALSNAPQSAAARQGQAVKLPVRAVAA"
     gene            1290..1763
                     /gene="puhC"
                     /locus_tag="AC16A0_04615"
     CDS             1290..1763
                     /gene="puhC"
                     /locus_tag="AC16A0_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex assembly protein PuhC"
                     /protein_id="HIR3104646.1"
                     /translation="MQPSPATAAPKLPTTVVVAFLCATLVFVAAARLGGMPPSPGMTG
                     QLTEAPEASRLLRFADAPDGSVRIIDAETGEDVAIVAPGTNHFLRGLMRGLNRARKSD
                     QVSYDVPFLLQRWPNNRLVLIDTHDGVALDLMAYGQTNAASFEPYLKRSEGGQAQ"
     gene            1760..2053
                     /locus_tag="AC16A0_04620"
     CDS             1760..2053
                     /locus_tag="AC16A0_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104647.1"
                     /translation="MNLFTGPREDVMCTVHVVNTFESLAAHVELDGNVEIKPGDEVLV
                     HGKPIVVPYGETAAERRKATIKRAGWIERKWVKMTGDLEFMELLEFSFSGEEL"
     gene            2050..3117
                     /gene="acsF"
                     /locus_tag="AC16A0_04625"
     CDS             2050..3117
                     /gene="acsF"
                     /locus_tag="AC16A0_04625"
                     /EC_number="1.14.13.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0048529 - magnesium-protoporphyrin IX
                     monomethyl ester (oxidative) cyclase activity [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     (oxidative) cyclase"
                     /protein_id="HIR3104648.1"
                     /translation="MTIETLGIRPADTLKKAQESTVLSPRFYTTDYAALNDMDVTSVR
                     KEWDALIAEMASDPNKSHFKRNAEWDHVTLESLPEGLRKEFVDFLVSSLTAEFSGCVL
                     YAEMRKRGSNPDMCELFKLMSRDEGRHAGFINDTLKDFKIGVDLGFLTKTKKYTYFKP
                     KFIFYATYLSEKIGYARYITIFRHLQAHPERRFHPIFKWFEQWCNDEFRHGEAFALLM
                     RANPELLSGLNKYWVKFFLLAVFATMYVRDHQRPHFHEALGIDPDDYGYKVFKITSEI
                     SRQVFPLELDIDNPAFFAGLAKLRQINDRNAVWQQKGGLTAKLGKLRTTAAAGLCFLR
                     LYMIPSKTNALPRDIRLAPTW"
     gene            3134..3958
                     /gene="puhE"
                     /locus_tag="AC16A0_04630"
     CDS             3134..3958
                     /gene="puhE"
                     /locus_tag="AC16A0_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative photosynthetic complex assembly protein
                     PuhE"
                     /protein_id="HIR3104649.1"
                     /translation="MAYALPIAYALFLWWFCTGLIFYLDSLPARTFRWSMLGASAVLA
                     TALGAIWWLRGDTGIVATYLAFTAAVLAWGFQEISLYMGYITGPRKHRCQAGCSGWRH
                     FRHAIEVNLWHELSIIAVALVIAWLVWDSQNLVAMWTYLLLWMMNLSARLNVFLGVRN
                     VSEEFVPARMEVLKSFLNRKPMNLLFPVSVTAATIGSVLLFLSAASAYTAHDVTSLSF
                     LGAMMTLALVEHWFLMLPLPVEKLFRWTLAVQHGPRRPAHATASEAVELKLVKTTP"
     gene            3990..>4679
                     /gene="hemA"
                     /locus_tag="AC16A0_04635"
     CDS             3990..>4679
                     /gene="hemA"
                     /locus_tag="AC16A0_04635"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HIR3104650.1"
                     /translation="MNYENFFKSELDGLREEGRYRIFANLERKVGSFPRATHRTTDTE
                     HEITVWCSNDYLGMGQNPAVIAAMHEAIDRCGAGAGGTRNISGTNHYHVLLEQELADL
                     HGKESALLFTSGYVSNWAALGTLASCIPDCVVLSDAGNHASMIEGIRHSRAERHIFKH
                     NDVADLERLLKHIDPDRPKLIAFESVYSMDGDIGPIREICDLADKYNAMTYLDEVHAV
                     GMYGPRGGGIAE"
BASE COUNT          897 a         1553 c         1366 g          863 t
ORIGIN      
        1 ggcccacatc acggtccagg gcttccccgc aattccttca ccaaagacct tcaagctcgc
       61 cgacggctcg accgttatgg cgccccgcgc cgagccgcag gactcgcgcc cggtgaaggc
      121 gacgccggtc ggcggctggc tgggtgcgcc actcgagccg gacggcaatc cgatgctgga
      181 tggtgtgggg cctgccgcct atgccgaacg cgcggatatt ccggatgcca ccattcacgg
      241 caccccgaag ctcctgccgc tgcgcgccgc cgacggcttc tacatcgata cccatgatcc
      301 cgatccgcgc ggcatgccgg tgatcggcgc cgacgggcgg cagggcggca tcgtccgcga
      361 tgtctggctc gaccgggccg aatacatcat ccgctatctt gaagtcgagg tcagcgacaa
      421 gaacggcaag cgcaacgtgc tgttgcctac cacaatgtcg cgcgtcagcg gctcgcgcaa
      481 ccaggtggaa gtgaagtcca tcctcggcaa gcagttcgcc aatgttccgg cccatcgcaa
      541 gccggacgtg gtcacgcgcc tcgaggaaga ccgcatctgt gcctactacg ccagcggaca
      601 cctctatgcc acgcccgaac gtgccgagcc cctgatatga gcgacgattt cgacttcgag
      661 cccgtaaggg ggttgcccga gatgctgcca gcaggagaac gcctgctctg gcagggcgcc
      721 ccgcgctggc aggatcttgc agtccatgcc tttcatgccc gcaaggtgat ctggtatttc
      781 ctggccattg ccatcgtcca agccgtcttc aggcttgccg acggatcctc gctttccgat
      841 gcatcctggc ccttcgtctg gctcctgccg atgggcggta tcgcggcggc gatcctgacg
      901 ttcctcgcct atgtcagttc acggacgacc gtctacagca tgaccaccaa gcgcttggtc
      961 atgcgcatcg gcatcgccct tccggttacg ctgaacctgc cattccggct ggtggacagc
     1021 gcctcgctcc gtctctatgc caacggctcg ggcgagattc cgctcaagct cgctgcctcc
     1081 gagcgagttg cctacctgtt gctctggccg catgcgcggc ccctgcattt ccgcaatccg
     1141 caaccttgcc tccgcgccat tccaaacgct gaccaggtgg caaagcttct ggcaacggcc
     1201 ctgagcaatg cgccgcagtc tgctgcggcc cgccagggac aggccgtgaa gttgccggtt
     1261 cgggcggttg cagcatgagg acggaaagca tgcagcccag ccccgcgacc gcggcaccga
     1321 aactcccgac caccgtggtt gtcgcctttc tttgcgcgac gttggttttc gtggcagcgg
     1381 cgcgcctcgg gggaatgccg ccgtcgccgg ggatgaccgg acagctgact gaggcccccg
     1441 aggcgtcacg gcttctgcgc ttcgccgatg ccccggacgg cagcgtgcgc atcatcgacg
     1501 ccgagaccgg tgaagacgtg gcgatcgtgg cacccggcac caatcacttc ctgcgcggcc
     1561 tgatgcgcgg cctcaaccgg gctcgcaaga gcgaccaggt ctcctacgac gtccctttcc
     1621 tgctgcagcg ttggcccaac aacaggctcg tgctgatcga cacccatgac ggcgtcgccc
     1681 tggacctcat ggcctatggc cagaccaacg cagccagttt cgagccctat ctgaaacgta
     1741 gtgagggagg acaagcacaa tgaacctctt taccggtcca cgcgaagacg tgatgtgcac
     1801 cgtacatgtc gtcaacacct tcgagagcct cgccgcccat gtcgaactgg acggcaatgt
     1861 ggaaatcaag cccggggacg aggttctcgt acacggcaag cccatcgtcg tgccctacgg
     1921 cgaaaccgca gccgaacgcc gcaaggccac gatcaagcgc gccggttgga tcgagcgcaa
     1981 atgggtcaag atgacgggcg acctggaatt catggaactg ctggaattca gcttctcggg
     2041 ggaggaacta tgaccatcga aacactcggc atccgcccgg cggacacgct caagaaggcg
     2101 caggaaagca cggtcctgag cccgcgcttc tacacgaccg actacgcggc gctgaacgac
     2161 atggacgtca cctcggtgcg caaggaatgg gatgcgctga ttgccgagat ggccagcgac
     2221 ccaaacaagt cccacttcaa gcgtaatgcc gaatgggacc acgtcacgct tgagagcctt
     2281 cccgagggcc tgcgcaagga gttcgtcgac ttcctggtca gctcgctgac agccgagttc
     2341 tcgggctgcg tgctctatgc cgaaatgcgc aagcgcggat ccaatccgga catgtgcgaa
     2401 ctcttcaagc tcatgagccg cgacgaggga cgccacgccg gcttcatcaa tgacacgctc
     2461 aaggacttca agatcggcgt cgacctcggc ttcctgacca agaccaaaaa atacacctat
     2521 ttcaagccga agttcatctt ctacgcgacc tatctctccg agaagatcgg atatgcccga
     2581 tacatcacca tcttccgcca cctccaggcc catccggagc ggcggttcca tccgatcttc
     2641 aagtggttcg agcagtggtg caacgacgaa ttccgccacg gcgaggcctt tgccctgctg
     2701 atgcgggcca atcccgaact tctcagcggg ctgaacaagt attgggtgaa gttctttctg
     2761 cttgccgtct tcgccacaat gtatgtgcgc gaccatcagc ggccgcattt ccacgaggcc
     2821 ctcggcatcg atcccgacga ctacggatac aaagtcttca agatcacctc ggagatctcg
     2881 cggcaggtct ttccgctgga gctcgacatc gacaacccgg ccttctttgc aggtctcgcc
     2941 aagctgcgcc agatcaacga ccgcaacgcg gtctggcagc agaagggcgg actgactgcg
     3001 aagcttggaa agctccgcac cacggccgcg gctggcctct gctttctgcg gctctacatg
     3061 atcccgtcaa agaccaacgc cctgccgcgc gacatccgcc tggcgccaac ctggtaggat
     3121 ccgcacgact ccgatggcct atgccctgcc catcgcctac gcgctctttc tctggtggtt
     3181 ctgcaccggg ctgatcttct acctggacag cctcccggcg cgaaccttcc ggtggagcat
     3241 gctgggagcg agcgccgttc tggcgacagc actgggtgcc atctggtggc tgcgcggcga
     3301 taccgggatc gtggccacct acctcgcctt cacggcagcc gttctggcct ggggcttcca
     3361 ggaaatcagc ctgtacatgg gatacatcac cggtccgcgg aagcatcgct gccaggcagg
     3421 ttgttcaggc tggaggcatt tccgccatgc catcgaagtc aatctctggc acgagctgtc
     3481 gatcatcgcc gtcgccctgg tcattgcctg gctggtgtgg gacagccaga accttgtcgc
     3541 catgtggacc tatcttctgc tctggatgat gaacctgagc gcgcgcctga acgtgttcct
     3601 tggcgtgcgg aacgtcagcg aggagtttgt ccccgcccgc atggaagtcc tcaagagctt
     3661 tctcaaccgg aagccgatga acctgctctt ccccgtctcc gtgacggcgg cgaccatcgg
     3721 gtcggtcctt ctcttcctct cggccgccag cgcctatacg gcacatgacg tcaccagcct
     3781 gagtttcctt ggcgcaatga tgacgctggc gttggtcgag cactggttcc tgatgctgcc
     3841 attgccggtc gagaagctct tccgctggac actggcggtg caacatgggc cgcggcggcc
     3901 tgcacacgct acggcatccg aagccgtgga actgaaactg gtaaagacaa caccctgaaa
     3961 gcaagcggga agatgcgcgg gaggatgaca tgaactacga gaacttcttc aagtccgaac
     4021 tggatggact ccgcgaggaa gggcgctacc gcatcttcgc caacctggag cgcaaggtag
     4081 gaagcttccc gcgggcgacg catcgcacga cagacacgga gcatgagatc accgtctggt
     4141 gctcgaacga ctacctcggc atgggccaga atccggcagt cattgcagca atgcacgagg
     4201 ccatcgaccg ttgcggggct ggcgcgggcg gaacacgtaa catctcgggc accaatcact
     4261 atcacgtcct gctggagcag gaactggccg accttcacgg caaggaatcc gccctgctct
     4321 tcacctcagg ctacgtatcg aactgggccg cgctcggcac gctggcttcc tgcatccccg
     4381 actgcgtggt gctgtccgac gctggaaacc acgcttcgat gatcgaaggc atccgtcaca
     4441 gccgtgccga acgccacatt ttcaaacaca atgacgtggc cgatctcgag cgcctgctga
     4501 aacacatcga tccggatcgg ccgaagctga ttgccttcga atcggtctac tccatggatg
     4561 gcgacatcgg cccgatccgc gagatctgcg accttgccga caagtacaat gccatgacct
     4621 acctcgatga agtccacgcc gtgggcatgt atggcccgcg cggcggcggc atcgccgag
//
LOCUS       DBNGVR010000210         4309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4202000, whole genome shotgun sequence.
ACCESSION   DBNGVR010000210 DBNGVR010000000
VERSION     DBNGVR010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4202000"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="AC16A0_04640"
     CDS             complement(<1..628)
                     /locus_tag="AC16A0_04640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3104651.1"
                     /translation="MTKLDSRLHAFRPDLADSRLAGRVAAARYAEGRVMQVAAPLVTV
                     HKSPSSDAMQLTQALMGERLHVFDEQEGWAWVQLAADSYVGYVSANALTPALTEATHR
                     VAVPATFLYPAANLKTQPAIAVTLNARLCATGMEGKFLKTAAGLFVYADHVASIDAYA
                     DDFVSVAERFRHVPYYWGGKSVQGLDCSGLVQLALEACGIACPRDSDMQ"
     gene            complement(640..984)
                     /locus_tag="AC16A0_04645"
     CDS             complement(640..984)
                     /locus_tag="AC16A0_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR3104652.1"
                     /translation="MSVDLNTAQALKLWHDVALALVQDDEPDLSLRQVSILLTIYLEA
                     PPHTVRDLARKLGVSKPVITRALDSMGRLELVSRRRDEKDRRNVLVQRTVKGALYLER
                     LADSIGNLARKL"
     gene            complement(1054..2433)
                     /locus_tag="AC16A0_04650"
     CDS             complement(1054..2433)
                     /locus_tag="AC16A0_04650"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR3104653.1"
                     /translation="MQDPKALLLAAGAAGRSIPVTVIAAPEWPQFKAGLDRRAAAWAA
                     AQGFGADPGRELAIPAADGSVSRVLAGATPEQPFALGRLARVLPAGTYRLEGAVGDSR
                     LAALAWCLEAYSFDAYRKRKPAAARLVCPAGVDRAEVLRSAAAIFMVRDLVNTPASDL
                     GPDQLEAAAREVARSHRARLRVVKGRALERGFPMVHHVGKASPRTPRLIDFSWGSPRA
                     PKVTLVGKGVCFDTGGLDIKPSSSMLLMKKDMGGAANVLGLAQMIMAAKLPVRLRVLI
                     PAVENSIAGNAFRPGDVWRSRKGLTVEIGNTDAEGRLILGDALALADEERPDLLVDMA
                     TLTGAARVALGPDIPPFYTDDDATAEALGKAAVAENDPLWRMPFWRPYNRLFESAVAD
                     LNNAGESGYAGSITAALFLRRFVERARTYIHFDIFAWTPQARPGSPRGGEAQGIRALF
                     ALIRNRYGK"
     gene            2551..3336
                     /locus_tag="AC16A0_04655"
     CDS             2551..3336
                     /locus_tag="AC16A0_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3104654.1"
                     /translation="MQSKASRPNIRRPAAILVMVAAAALAGCQQKSAGLGSIDSMTTA
                     STVPANYEQTTELAKKWQSDTKNLKKGMAYANALDSIGQTQKQLEVLSVLYKSHPDNP
                     QVAALYGKKLLASGRSNEAVVVLETATMLNNKDWRSYSALGSAYDQQGIYDKARTAYQ
                     QALTIEPDNLTVLNNLGMSYALQGDLKEAEKTLRMADALPKAASEPRIRQNLALVVGL
                     QGRFEEASAIASKDLPPQQVEANMAYLKTMLSQPNTWQQLQEG"
     gene            3417..3683
                     /locus_tag="AC16A0_04660"
     CDS             3417..3683
                     /locus_tag="AC16A0_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104655.1"
                     /translation="MNHRHRKILHSLFSHPLPSNIHLHDVETVFRELGAELGHTGHGR
                     LSVSLKGQSASLHGSDHGLSKDEVSHVRKFIESCGIEPSRDFPI"
     gene            complement(3762..>4309)
                     /locus_tag="AC16A0_04665"
     CDS             complement(3762..>4309)
                     /locus_tag="AC16A0_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3104656.1"
                     /translation="PPMRFAAAGVGIIFGYYLPSIFLKNLVKRRQDSIKRAWSDALDL
                     MLICVESGMSIEMGFQRVAREIGSASVPLAEELTLTVAELSYLQDRRKAYENLGKRTG
                     LATVKSVVTSLIQSERYGTPLGTALRVLAQENRDMRMSDAEKKAAALPPKLTVPMILF
                     FLPVIFVIILGPSIIRVMNIN"
BASE COUNT          811 a         1407 c         1401 g          690 t
ORIGIN      
        1 cctgcatgtc cgagtcgcgc gggcaggcga tgccgcaggc ctcgagcgct agctgcacaa
       61 ggccggaaca gtcgagaccc tgaacggact tgccgcccca gtaatagggc acatggcgga
      121 aacgttcggc gacgctgacg aaatcatccg cgtaggcatc tatcgacgcc acatgatcgg
      181 cgtagacgaa gaggccggcg gcggtcttca ggaacttgcc ctccatacct gtcgcacaaa
      241 gcctggcgtt gagcgtgacg gcgatggccg gctgggtctt caggttcgca gccggataga
      301 ggaaggttgc aggaacagcg acgcgatgcg tggcctcggt cagcgccggc gtgagggcat
      361 tggccgacac ataaccgacg taggaatcgg ccgcgagctg aacccaggcc catccttcct
      421 gctcgtcgaa gacatgcagg cgctcgccca tcagcgcctg ggtgagctgc atggcatccg
      481 acgacggcga cttgtgaacc gtcaccagag gcgcggccac ctgcatgacg cggccttccg
      541 catagcgcgc ggccgcgaca cggccggcca ggcggctgtc ggccagatcc gggcgaaagg
      601 catggagacg ggaatcgagc tttgtcatga ctcccgcgtc tagagctttc gcgcgagatt
      661 gccaatcgag tcggcgagcc tctccagata gagcgcgccc ttcacggtgc gctggaccag
      721 cacattgcgg cggtccttct cgtcgcggcg gcgcgacacc agctctaatc ttcccatgct
      781 gtccagcgcc cgggtgatga ccggcttcga gacgcccagc ttgcgcgcga gatcgcgcac
      841 ggtatgcggc ggggcctcga gatagatggt gagcaggatc gagacctggc gcagcgagag
      901 gtcaggctca tcgtcctgca ccagggcaag ggccacgtcg tgccagagtt tcagggcttg
      961 cgccgtattg aggtccaccg acatgaaaca cgcctccgca acggattgtt acgggtccgt
     1021 atcagatttg cgcgaatcct gtccgggcgc aagctacttt ccgtaccggt tccggatcag
     1081 cgcgaagagc gcgcggattc cctgggcttc gccgccgcgc gggcttccgg gccgggcctg
     1141 cggggtccag gcgaagatgt cgaagtgaat gtaagtccgc gcccgctcga caaagcgcct
     1201 gaggaaaagc gccgccgtga tcgaccccgc atatccggac tcgccggcgt tgttgaggtc
     1261 ggcgacagcc gactcgaaga ggcggttgta ggggcgccag aagggcatgc gccacaatgg
     1321 atcgttctcg gccaccgcgg ccttgcccag ggcttcggcg gtggcgtcgt catcggtgta
     1381 gaagggtgga atgtccggcc ccagcgccac ccgcgcagca ccggtgaggg ttgccatgtc
     1441 aacgaggaga tcgggccttt cctcgtcggc cagggccagc gcatcgccga ggatcagcct
     1501 gccctcggca tcggtgttgc cgatctcgac cgtgagcccc ttgcggctcc gccagacatc
     1561 gcccggacgg aaggcgttgc cggcgatgga attctccacc gccggaatca agacccgcag
     1621 gcggaccgga agtttcgccg ccatgatcat ctgggcgagg ccaagaacat tggcggcgcc
     1681 gcccatgtcc tttttcatca gcagcatgct tgaggagggc ttgatgtcca ggccaccggt
     1741 atcgaaacag actcccttgc cgacgagcgt cacctttggc gcccgcgggc tgccccatga
     1801 gaagtcgatc aggcgcggcg tccgcgggct ggccttgccg acgtgatgga ccatgggaaa
     1861 tccccgttcc agggccctgc ccttcaccac gcggagcctg gcgcgatgcg accttgccac
     1921 ttcacgcgcc gcggcctcga gctgatccgg ccccaggtcg gaggccggcg tgttcaccag
     1981 gtcgcgcacc atgaagatgg cagcggcgct gcgcaggacc tcggcccgat cgacccctgc
     2041 cggacagaca agccgggccg ccgccggctt gcgcttgcgg taggcatcga agctgtaggc
     2101 ctcgaggcac caggccaggg cggccagccg cgaatccccc acggcccctt cgagccggta
     2161 ggttccggca ggcaggacgc gcgccagccg gccgagcgca aagggctgct ccggcgtggc
     2221 tccggccagc acgcggctca ccgacccatc ggcggccgga atggcaagct cgcgtcccgg
     2281 atcggccccg aagccctgtg ccgcggccca ggcggcggcg cggcggtcca gtcccgcctt
     2341 gaattgcggc cactccggcg ccgcgatcac ggtcacggga atgctgcggc cagccgcacc
     2401 ggcggccagg aggagggctt tggggtcctg catgaatcgc tccagctgat tgttcccttt
     2461 cttaacgttg attgacgatc atggttaagc gtttattgac gagttgccgc ccatgatccg
     2521 gattcgagac gtgtattcgg agcagagtcg atgcaatcca aagcttcccg gccgaacatc
     2581 aggcggccgg cggcaattct ggtgatggtg gccgcggcgg cgctggcggg ctgccagcag
     2641 aagtcggccg gccttggcag catcgattcg atgaccaccg catcgacggt gccggccaac
     2701 tatgagcaga ccaccgaact ggccaagaaa tggcagtcgg atacgaagaa cctgaagaag
     2761 ggcatggcct atgccaatgc cctggacagc atcgggcaga cccagaagca gctggaggtc
     2821 ctgtcggtcc tgtacaagtc ccatcccgac aatccccagg tcgcggccct ctacggcaag
     2881 aagctgctgg cctcgggcag gtccaacgag gccgtcgtgg tgctcgagac cgcgaccatg
     2941 ttgaacaaca aggactggcg gagctactcc gccctcggct cggcctatga ccagcagggc
     3001 atctacgaca aggcgcgcac cgcctaccaa caggccctca ccatcgagcc cgacaatctg
     3061 accgttctca acaatctggg catgtcctac gcgctccagg gcgacctcaa ggaggcggaa
     3121 aagaccttgc gcatggccga cgccctgccg aaagcggcca gcgagccgcg catccggcag
     3181 aatcttgcgc tcgtggtcgg cctgcagggc cgcttcgagg aggccagcgc catcgccagc
     3241 aaggatcttc cgccgcagca ggtcgaggcc aacatggcct atctcaagac gatgctgtcg
     3301 cagcccaaca cgtggcagca gcttcaggaa ggctgaccgc gggccatttg atcggcgtca
     3361 agccgcagca cccgcgccgc gcatagattg catctgtcac ccacggagac agatacatga
     3421 accaccgcca ccgcaagatc ctgcacagcc tcttcagcca tcccctgccc agcaacattc
     3481 atctgcatga cgtcgagacc gtctttcgcg aactgggggc ggagctgggc cacaccggac
     3541 atggccggct cagcgtgtcc ctgaaggggc agtcggcgag cctccatggc tcggaccacg
     3601 ggctgtcgaa agacgaagtg agccacgtcc gcaagttcat cgaaagttgc ggcatcgagc
     3661 cttcccgcga ctttcccatc tgaaatcacg cattcgcaga aacgggacag gcgcggaaac
     3721 cggttccgcg cctgtctcat gtcttgccgt gggccgcggc gtcagttgat gttcatcaca
     3781 cggatgatcg agggcccgag gatgatcacg aaaatgacgg gcaggaagaa caggatcatc
     3841 gggaccgtga gcttcggcgg caaggcggca gccttcttct cggcgtccga catgcgcatg
     3901 tcacggtttt cctgagccag gacacgcagg gccgtgccaa gcggcgtgcc gtagcgttcc
     3961 gactggatca gcgaggtcac gaccgatttg acggtcgcca ggccggttcg cttgccaaga
     4021 ttttcatagg ccttgcggcg atcctgcaga taggagagct cggcaaccgt gagcgtcagc
     4081 tcctcggcca gcggcaccga cgccgagccg atctcgcgcg ccacgcgctg gaagcccatt
     4141 tcgatcgaca tacccgattc gacgcagatc agcatcaggt cgagcgcatc cgaccaggcg
     4201 cgcttgatcg aatcctggcg gcgcttcacc aggttcttga ggaagatcga cggcaggtag
     4261 tagccgaaga tgatgccgac gccggccgcg gcgaagcgca tcggcgggg
//
LOCUS       DBNGVR010000211         4037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3811143, whole genome shotgun sequence.
ACCESSION   DBNGVR010000211 DBNGVR010000000
VERSION     DBNGVR010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3811143"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="AC16A0_04670"
     CDS             complement(<1..712)
                     /locus_tag="AC16A0_04670"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104657.1"
                     /translation="MTIKRVGLIGVGLMGHGIGKNILAKGYELTVMAHRNRDPVEDLI
                     KRGAHERNNPLAIAKDSDLVILCVTGTPQIEQLVYGEYGLMGAVRPGLVIADCSTAEP
                     DSTIRIAADVEAKGGNFVDTPMTRTPKEAEAGKLGLMTGGPDEVIARIRPVLECFADT
                     IVHAGAVGSAHQLKLINNFLAICHATVAAEAITVAAKAGVDMKAMRDIVMAGGAASVM
                     FGRLINVPLSDDDSHAQFA"
     gene            complement(709..1188)
                     /locus_tag="AC16A0_04675"
     CDS             complement(709..1188)
                     /locus_tag="AC16A0_04675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3104658.1"
                     /translation="MTPVIRDMRDDEADQVAAMVHGLARHLGTGFVPRVTGASLRSSR
                     DLIDVMVAEDGGALIGACLGLMTFSTWRGARGLYIVDLFVTPQARGRNIGRDLLRRQA
                     RKFEARGARFIKLEVDESNTGAARFYARLGFRKKSEDRLHILEQDELQDFISTGDDT"
     gene            complement(1185..2216)
                     /locus_tag="AC16A0_04680"
     CDS             complement(1185..2216)
                     /locus_tag="AC16A0_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3104659.1"
                     /translation="MITVLDETQRTHAPREFIVSGRPQPIPERPERIDMLLAGVRQAG
                     SRIVAPPEIFGDTLATVHDRRYIQFLSTVWERWRRIPDAAASPSANVFALGRSTLPPV
                     SYPDSVVGQCGYHLGDGSCPIPAQTWAAARASAATAVHGAMLVLAGERLVYALCRPPG
                     HHAAADVAAGFCYFNNAALAAETLTRAGRRTAILDIDVHHGNGTEAIFYDRADVLTVS
                     IHADPKRFYPFFWGHAAETGRGAGTGYNLNLPLARGTTIKPYRAALDTALARIADFAP
                     EILVLAAGLDIAVDDPFQGFAIATPEFAEVGQAIGSAGLPILVVQEGGYPSPSLGANL
                     ASLLTGLGA"
     gene            2290..3636
                     /locus_tag="AC16A0_04685"
     CDS             2290..3636
                     /locus_tag="AC16A0_04685"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3104660.1"
                     /translation="MSATASLPQHIHSTGQLPKVAWAKGSYVHDVDGKRYIDGSGGPA
                     VYCIGHSNEEVNDAIRQQLDRIAHGYRYNFTSDALEELTDIIRQRCGGTLNHMVYVTG
                     GSEAVESCLKLALQYHAARGEMSRRRFIARERSWHGNTLGALSVSGFLERKKAFEGSL
                     LDVSHLSPANAYRPVAGATAETVGEACAKELEDEILRVGPEKVCAFIFEPVVGATGGC
                     VPAPPGYAKRVREICDRHGVLMIADEVMCGAGRVGTWRALEHDGVEPDIMSVAKGLAA
                     GYLPLGAAIYTDTVADAIHGKHGAPMTGHTFTGHTACCAAGVAVQRIVAREKLVERVR
                     ANGPRLTALMAAALSGVDAVGDIRGRGYFQAIELVADRATKTPFPAERKLFMKIRQKA
                     FDNGLICYPVGGNADGVNGDVVILAPPYNVTDAELTEIVDKTALSIRQVLAAEGLG"
     gene            complement(3687..>4037)
                     /locus_tag="AC16A0_04690"
     CDS             complement(3687..>4037)
                     /locus_tag="AC16A0_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3104661.1"
                     /translation="TVDFVPHTSELVFEETFGPVIPIVRVPNDDAEVIRISNSTPFGL
                     SSGVCTNSLPRITRFIEGLEVGTVNIWEVPGYRIEMSPFGGIKDSGNGIKEGVMEAMK
                     FFTNVKTWSLPWPA"
BASE COUNT          687 a         1267 c         1376 g          707 t
ORIGIN      
        1 tggcgaactg cgcgtggctg tcgtcgtccg acagcggcac gttgatgagc cgcccgaaca
       61 ttaccgaggc cgcaccgccc gccatgacga tgtcgcgcat cgccttcatg tcgacgccgg
      121 ccttggcagc gaccgtgatc gcttcggccg caacggtagc gtggcagatc gccaggaaat
      181 tgttgattag cttcagttga tgggccgagc ccacggcacc ggcatggacg atggtatcgg
      241 caaagcactc gagcacgggg cggatgcggg cgatgacttc gtccggtccg ccggtcatga
      301 ggcccagctt tccggcttcc gcctcctttg gcgtgcgcgt catcggcgtg tcgacgaagt
      361 tgccgccctt ggcctcgaca tcggccgcga tgcggatggt cgagtccggc tcggcggtgg
      421 agcagtccgc gatgacgagg ccggggcgca ctgcgcccat caggccatat tcgccgtaga
      481 ccagctgctc gatctgcggc gtgccggtaa cgcagaggat gacaaggtcg ctgtccttgg
      541 cgatggcgag cgggttgttg cgctcatgcg cgccgcgctt gatcaggtct tccaccgggt
      601 cgcggttgcg atgcgccatg accgtgagtt catagccttt ggccaggatg ttcttgccga
      661 tgccgtgccc catgaggcca acgccgatca gcccgacgcg cttgatggtc atgtgtcgtc
      721 tcccgttgaa atgaagtcct ggagttcatc ctgctccaga atgtgaagcc tgtcttcgct
      781 cttcttgcgg aatcccagcc gcgcgtagaa acgcgcggcg ccggtattgg attcgtccac
      841 ttccagtttg atgaagcgcg ctccgcgagc ttcgaacttg cgggcctggc ggcgcaggag
      901 gtcccggccg atgttgcggc cgcgggcttg cggcgtcacg aagaggtcga cgatgtagag
      961 gccgcgcgcg ccgcgccagg tggagaaggt catcagaccg aggcaggccc cgatgagtgc
     1021 tccgccatcc tcggctacca ttacgtcgat cagatcgcgc gaggaccgca aggatgcacc
     1081 agtgacgcgc ggcacgaaac ccgttccgag atgccgggcg aggccatgca ccatggcggc
     1141 cacctggtcg gcctcgtcgt cccgcatgtc ccggatcacg ggggtcatgc gccgaggccg
     1201 gtgagcaggg atgcgaggtt ggcgccaagg ctcggtgacg gatagccgcc ttcctgaacg
     1261 acaaggatgg gaaggccggc gctcccgatg gcctgtccga cctcggcgaa ctcgggcgtg
     1321 gcgatggcga agccctggaa gggatcgtca accgcgatgt cgagtccggc cgccagcacg
     1381 agaatctccg gtgcaaaatc cgcgatgcgg gcgagggcgg tgtcgagcgc agcgcgatag
     1441 ggcttgatgg tggtgccgcg tgccagcggc aggttgagat tgtagcccgt tcccgctccg
     1501 cgcccggttt ccgcggcatg gccccagaag aacggataga agcgcttcgg atcggcatgg
     1561 atggagaccg tcagcacgtc ggcacggtca tagaagatcg cctccgtgcc attgccgtga
     1621 tgcacgtcga tgtcgagaat ggcggtgcgc cgcccggcgc gggtcagcgt ctcggcggcg
     1681 agcgcggcat tgttgaagta gcagaagccg gccgccacgt cggcggcggc atgatggccg
     1741 ggaggcctgc agagcgcata gacaagccgt tcgccggcca gcaccagcat ggcgccatgc
     1801 acggcggttg cagcactcgc ccgggccgcg gcccatgtct gcgcagggat cgggcaggat
     1861 ccgtcgccca ggtggtagcc gcactggccc acgacgctgt cggggtagga gaccggcggc
     1921 agggtcgacc ggccgagcgc aaagacattc gcggaggggc ttgcggcggc atccggaatg
     1981 cgccgccagc gttcccagac ggtcgagagg aactgaatgt agcgacggtc atgcacggtg
     2041 gccagcgtgt cgccgaagat ctcgggcggc gccacgatcc ggcttcccgc ctgacgtacg
     2101 cccgcgagca gcatgtcgat ccgctcgggc cgttccggaa tgggctgcgg cctgcccgac
     2161 acgatgaatt cgcggggcgc atgcgtgcgt tgcgtttcgt ccagaaccgt gatcaccggt
     2221 ttgaagtgcc cctcgatcct ctgctaagag cggctgtccc gcatcccctc attgaaagcg
     2281 aatccgcgca tgtccgcaac cgcttccctg ccccagcata ttcattcaac cggccagctt
     2341 ccgaaagtgg cctgggcgaa gggcagctat gtccatgacg tcgacggcaa gcgctacatc
     2401 gacggctcgg gcggtcccgc cgtctactgc atcgggcatt cgaacgaaga ggtgaatgac
     2461 gcgatcaggc agcagcttga ccgtatcgcc catggctatc gctacaactt cacctccgac
     2521 gcgctggagg aactcaccga catcatccgc cagcgctgcg gcgggaccct gaaccacatg
     2581 gtctatgtca ccggcggttc cgaggcggtt gaatcctgcc tgaaactcgc gctgcagtat
     2641 catgccgcgc gcggcgagat gagccggcgt cgcttcatcg cgcgcgaacg gtcgtggcat
     2701 ggcaatacgc tgggggcgct ctcggtctcg ggtttccttg agcgcaagaa ggcctttgag
     2761 gggtcgctgc tggacgtctc gcacctttcg cctgccaatg cctatcgtcc cgttgcgggc
     2821 gcgacggccg agacggttgg cgaagcctgc gcgaaggaac tcgaggacga gatcctgcgg
     2881 gtcggtccgg agaaggtctg cgccttcatc ttcgagcccg tcgtgggagc caccggcggc
     2941 tgcgtgccgg cgcctcccgg ctatgcgaag cgggtccgcg agatctgcga ccgccatggc
     3001 gtactgatga ttgcggacga ggtcatgtgc ggcgcgggcc gcgtcggcac ctggcgggcg
     3061 ctggaacatg acggcgtcga gcccgacatc atgtcggtgg ccaagggtct tgccgccggt
     3121 tacctgccgc tgggtgccgc gatttatacc gacacggtgg ccgatgcgat ccatggcaag
     3181 catggcgcgc cgatgacggg gcacaccttc accggccata ccgcctgctg cgcggctggc
     3241 gttgccgtgc agcgcatcgt cgcacgcgag aagctggtgg aacgcgtgcg cgccaacggc
     3301 cccagactca cggccctgat ggccgctgcc ctttccggtg tcgatgcggt tggtgacatc
     3361 cgcggccgcg gctacttcca ggccatcgaa ctggtcgcag atcgcgcgac gaagactccc
     3421 tttccggctg agcgcaagct cttcatgaag atacgtcaga aggccttcga caatgggctc
     3481 atctgctatc cggtgggcgg caatgccgac ggcgtcaacg gcgacgtcgt gatcctggcg
     3541 ccgccctaca acgtgaccga tgccgaactg accgagatcg tcgacaagac ggccctgtcg
     3601 atcaggcagg tcctggcggc ggaaggtctg ggctagggcc gcccagcaga ttgacgatga
     3661 cgagggttcc cgggttcaat cctgcctcaa gcaggccagg gcagtgacca ggtcttcaca
     3721 ttggtgaaga acttcatggc ctccatgacg ccttccttga tgccgttgcc ggagtccttg
     3781 atgccgccga atggcgacat ctcgatacga tagcccggca cttcccagat gttcacggtg
     3841 ccaacctcga ggccttcgat gaaacgcgtg atgcgcggca ggctgttggt gcagacgccg
     3901 gaggagaggc caaacggcgt ggagttggaa atgcggatca cttcggcatc gtcgttgggc
     3961 acgcgcacga tggggatgac cggcccgaag gtctcttcga agaccagctc cgaagtatgc
     4021 ggcacgaagt cgacggt
//
LOCUS       DBNGVR010000212         2390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_667587, whole genome shotgun sequence.
ACCESSION   DBNGVR010000212 DBNGVR010000000
VERSION     DBNGVR010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_667587"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="AC16A0_04695"
     CDS             complement(<1..627)
                     /locus_tag="AC16A0_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HIR3104662.1"
                     /translation="MTLTAMLAQAQGGRLFAQVADAIGLDEATTRAAMMEICPAIISE
                     LKAAAEGDDSLLETLARHLAEGNEGSVLDDPGVLTGAESIKDGNAILAAIYGSKAGAM
                     EDVKTLVGGVPAAKLSKLAAVSATAAVSALMQSHAITPLAGVQRAAGTGGGILSVFID
                     ALIKGMVRAATSELNRQVRNVVKPRKAPAKRNAAAKRAPARKTPARKTA"
     gene            complement(624..1961)
                     /locus_tag="AC16A0_04700"
     CDS             complement(624..1961)
                     /locus_tag="AC16A0_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3104663.1"
                     /translation="MTGSTMNHVVVMTLTGAFGLMAMFMVDLADLFFLSLLNQTEVTA
                     AIGYAGTIVFTNLSVSIGIGIAAAALVATNLGAGKSERAREFATSSLLFALILSAIIT
                     IGIAAGSGWLLWLLGAQGEALRLAQAFIWTLTPGYILLTGAVCCSFILRGLGDPRRAM
                     YITLSAAVITALVDPLFIFGFGWGIQGAAAANVLGYSVSFAIGIHGVARVHRFINPLR
                     LSGLKRDFPAIWAIALPAMLTQLATPFANAYITHEVAPFGDEAVAASAIIGRLVPVAF
                     GMIFALSGSVGPIIGQNFGARKFDRVIQTLKAGLLFSAISTLLTSSILFLFRHEIAEA
                     FRASGRTVDIVVFFCTFIAISWAFAGAQFVSNAAFNNLGRANLSTMFNWGKSTIGTIP
                     FAMVGASYAGPEGVLAGTAVGSIIFGVASVYAAFRIVGRLAQQTSQASEGSAT"
     gene            complement(1976..>2390)
                     /locus_tag="AC16A0_04705"
     CDS             complement(1976..>2390)
                     /locus_tag="AC16A0_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3104664.1"
                     /translation="SRIGHPIDRAGWIEARRSAMAPWPEMLDLARQIGQQSRIAIYTN
                     NGPLTKAALPELFPEAAEIFPERYYSFEFATKKPDPLSFTRLTSHMEVEPQSCWFVDD
                     KRSNVQGARMAGLAGHHFRSYDQFRTEALSLGFEL"
BASE COUNT          433 a          746 c          822 g          389 t
ORIGIN      
        1 cgccgtcttg cgggccggtg tcttgcgcgc cggtgcacgc ttcgccgcgg cgttacgctt
       61 cgcgggggcc ttgcgcggct tgaccacgtt gcggacctgc cggttgagct cgctggttgc
      121 ggcccgcacc atgcccttga tcagggcatc gatgaagacg ctgagaatgc cgccgccggt
      181 gccggcggcc cgctgcacgc cggccagcgg cgtgatggca tgcgattgca tcagcgcgct
      241 gaccgccgcg gtggcgctga ccgccgcgag tttggacagc tttgctgcgg gcacgccgcc
      301 gacaagcgtc ttgacgtcct ccatcgctcc ggccttcgag ccatagatgg cggccaggat
      361 ggcgttgcca tccttgatcg actcggcccc cgtgagaacc ccggggtcat ccagcaccga
      421 accttcattg ccctcggcca gatgccgggc cagggtttcc agaaggctgt cgtcgccctc
      481 ggcggccgcc ttcagctcgg aaatgatcgc ggggcagatt tccatcatgg cggcgcgcgt
      541 cgtggcttcg tcgagaccga ttgcatcggc aacctgcgcg aacagtcgtc caccctgtgc
      601 ctgtgccagc atggccgtca gtgtcatgtc gcgctcccct cgctggcctg gcttgtctgc
      661 tgtgcaagcc ggccgacaat cctaaaggct gcatagaccg acgcaacgcc aaaaatgatg
      721 gacccgacgg ctgtgccggc cagtacgcct tccggccctg cgtagctggc gcccaccatg
      781 gcgaagggga tggtcccgat ggtcgacttc ccccagttga acatggtcga cagattggcg
      841 cggccaagat tgttgaaggc tgcgttcgac acgaattgcg ccccggcaaa ggcccagctg
      901 atggcgatga aggtacagaa gaataccacg atgtcgaccg tgcggcccga ggcacggaag
      961 gcttcggcga tctcatgccg gaacaggaag aggatggacg aggtgagcag ggtagagatc
     1021 gccgagaaca gcaggccagc cttcagggtc tggatcaccc ggtcgaactt tcgcgcgccg
     1081 aagttctggc cgatgatcgg cccgaccgat ccggacaggg cgaagatcat gccgaaggca
     1141 accggcacca gccgtccgat gatggccgag gcggcaacgg cctcgtcgcc gaagggcgcc
     1201 acctcatggg tgatgtaggc attggcgaag ggcgtcgcca gttgggtcag catggccgga
     1261 agggcaatcg cccagatggc cgggaagtcg cgcttcagcc cggagagccg cagggggttg
     1321 atgaagcggt gtaccctggc cacaccgtga atgccgatgg cgaacgagac ggagtagccg
     1381 aggacatttg ccgcagccgc gccctggatg ccccagccga agccaaagat gaagagcggg
     1441 tcgacgaggg cggtgatcac cgccgcggac aaggtgatgt acatcgcccg gcgcggatcg
     1501 ccgagcccgc gcaggatgaa ggagcagcag acggcccccg tcagcaggat gtatccaggc
     1561 gtcagcgtcc atatgaaagc ctgcgccagc cgcagcgcct cgccctgggc gcccagcagc
     1621 cacagcagcc accccgagcc tgccgcgatg ccgatggtta tgatggccga aaggatcagg
     1681 gcgaagagca gcgagctcgt ggcgaattcg cgggcacgct cggatttgcc ggcgcccagg
     1741 ttggtcgcaa ccagcgctgc tgccgcgatc ccgatgccga tgctgaccga caggttggtg
     1801 aagacgatcg tcccggcata tccgatggct gccgtcactt cggtctggtt caggaggctc
     1861 aggaagaaca ggtccgccag atcgaccatg aacatggcca tcaggccgaa ggcgcccgtg
     1921 agcgtcatga ccaccacgtg gttcatggtg gagccggtca cgaaacgcgc cggggtcaaa
     1981 gctcgaatcc gagactgagc gcttccgtgc ggaactggtc gtaggagcgg aaatgatgac
     2041 cggccagccc tgccatgcgg gcgccctgga cattggatcg cttgtcgtcg acgaaccagc
     2101 aggactgcgg ctccacctcc atgtgcgagg tcaatcgggt gaagctcagg ggatcgggct
     2161 tcttggtcgc aaactcgaag gagtagtagc gctcgggaaa gatctcggcc gcctcgggga
     2221 acagctcggg gagggccgcc ttggtgagtg gcccgttgtt ggtatagatg gcgatccgcg
     2281 actgttggcc gatctggcgc gcgaggtcga gcatctcggg ccatggcgcc atggcgctgc
     2341 ggcgggcctc gatccagccg gcccggtcga tgggatgtcc gatccggctg
//
LOCUS       DBNGVR010000213         1790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2826983, whole genome shotgun sequence.
ACCESSION   DBNGVR010000213 DBNGVR010000000
VERSION     DBNGVR010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2826983"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1790)
                     /gene="dnaE"
                     /locus_tag="AC16A0_04710"
     CDS             complement(<1..>1790)
                     /gene="dnaE"
                     /locus_tag="AC16A0_04710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3104665.1"
                     /translation="QMAALAKDARMPAVAITDTGNLFGALEFSDALAEKGIQPIIGCT
                     IKVDMADAPKEGSLRPQQGLRRMPSLALLAKDETGYGNLMKLSSQAYLSSPDNAEHHV
                     PIADLALRSEGLICLTGGPAGPLNEALVAGQMSLARTYAERLKDMFGDRLYVELQRHG
                     TEAERAAEAGLVEIAYALDLPLVATNEAYFAKASDYVAHDALICIAEGEAIATEDRRR
                     LTPEHYFKSQADMAALFADLPEAVENTVEIAMRTAFRVTSRAPILPRFAEDDEAEELK
                     RQAHEGLVQRLETRGLVEGKVREDYVSRLDFELDVISKMQFPGYFLIVSDFIKYAKSK
                     GIPVGPGRGSGAGSMAAYALTITDLDPFRFNLLFERFLNPERVSMPDFDIDFCQDRRD
                     EVIAYVQQRYGADRVAQIITFGKLQARMVTRDVGRVLQMPYGQVDRLSKLIPNNPANP
                     VTLQQAIDGEPKLQEERDRDPSVKTLFDIALKLEGLYRNASTHAAGVVIGDRPLQELV
                     PLYRDPRSSSPATQYNMKYVEKAGLVKFDFLGLKTLTVIEKAQKLIRVREPDFDIDRI
                     PYDDKKTYDMLGHGDTVGVFQLESSGMRDVL"
BASE COUNT          303 a          563 c          577 g          347 t
ORIGIN      
        1 agaacatcgc gcatgcccga gctttccagc tggaacaccc ccacagtgtc gccatggccc
       61 agcatgtcgt aggtcttctt gtcgtcgtag ggaatgcggt cgatatcgaa gtccggttca
      121 cgcacccgga tcagcttctg cgccttctcg atgacggtca gggtcttgag tccgaggaag
      181 tcgaacttta ccagtccagc cttctccacg tacttcatgt tgtactgggt ggccggactc
      241 gacgagcgcg gatcgcgata gagcggcacc agttcctgca gcggcctgtc gccgatcacc
      301 acgcctgccg catgggtcga cgcattgcga tagagtcctt ccagcttcag cgcgatgtcg
      361 aagagcgtct tcacggaggg atcgcggtcg cgctcttcct gcagcttggg ctcgccgtcg
      421 atggcctgct gcagcgtcac cgggttggcg gggttgttgg gaatgagttt ggacagccgg
      481 tcgacctgtc cgtagggcat ctgcagcacc cggcccacat cgcgtgtcac catgcgggcc
      541 tgcagcttgc caaaggtgat gatctgggcc acgcgatcgg cgccgtagcg ctgctgcaca
      601 taggcgatga cctcgtcgcg ccggtcctgg cagaagtcga tgtcgaagtc cggcatcgag
      661 acgcgctcag gattgaggaa tcgctcgaaa agaaggttga accggaaggg atccaggtcg
      721 gtgatcgtca acgcgtaagc cgccatggag cccgcgcccg aaccgcgccc cggccccact
      781 ggaatgccct tcgacttcgc atacttgatg aagtcggaca cgatcagaaa gtagcccggg
      841 aactgcatct tgctgatgac gtcgagttcg aagtcgagcc ggctgacata gtcctcccgg
      901 accttgccct ccaccagtcc gcgggtttcg aggcgctgga caagaccctc atgtgcctgc
      961 cgcttcagtt cctcggcctc gtcgtcctcg gcaaagcggg gcagaatggg cgcgcgcgac
     1021 gtcacccgga aggccgtccg catggcgatc tcgacggtgt tctccaccgc ttcgggaagg
     1081 tcggcgaaga gagccgccat gtcggcttgc gacttgaaat agtgttccgg cgtcaggcgg
     1141 cggcggtcct cggtggcgat ggcctcaccc tcggcgatgc agatcagcgc atcgtgcgcc
     1201 acatagtccg aggccttggc gaaataggcc tcattggttg ctacgagggg caggtccagc
     1261 gcataggcaa tctcgacgag ccccgcttcg gcggcacgtt cggcctcggt gccatggcgc
     1321 tgcagctcaa catagagccg gtcgccgaac atgtccttca ggcgctcggc ataggtcctg
     1381 gcaaggctca tctggcccgc caccagggct tcgttcaagg gtcctgcggg acctccggtc
     1441 aggcagatga ggccctcgct gcgcagcgcg agatcggcaa tcgggacgtg atgctcggca
     1501 ttgtcgggcg acgacaggta ggcctggctg gacagcttca tcaggttgcc gtagccggtc
     1561 tcgtccttgg ccagcagtgc gagcgacggc atgcggcgaa gtccctgctg gggtctgagg
     1621 ctcccctcct tcggcgcatc ggccatgtcc accttgatgg tgcagccgat gatcggctgg
     1681 atgccctttt cggccagcgc gtcggagaat tcgagcgccc cgaacagatt gccggtgtcg
     1741 gtgatcgcca ccgccggcat ccgcgcgtcc ttggcgagtg ctgccatctg
//
LOCUS       DBNGVR010000214         2736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_867163, whole genome shotgun sequence.
ACCESSION   DBNGVR010000214 DBNGVR010000000
VERSION     DBNGVR010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_867163"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="AC16A0_04715"
     CDS             <1..600
                     /locus_tag="AC16A0_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR3104666.1"
                     /translation="KLDINNVDWKNVDRSKIKFDKNQLNKIDRSKVKNNVKNNDRNKI
                     TNKAGDLKKNRPTTLPGKGNRPTKDVRASTLEGLKKQPGARPADKMARPGQGAGDKMA
                     RPGQGVTNKRPEGGKIDRPVGKPKPAARPDNRPKTPSPMGDMKRGKNAKFDSNRGAKS
                     MGGGGYKRPSGGGGGQKMKKRPMPKGGGGGRGGGGRGRR"
     gene            636..1574
                     /locus_tag="AC16A0_04720"
     CDS             636..1574
                     /locus_tag="AC16A0_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HIR3104667.1"
                     /translation="MKHLISSALLGTALVFGTVSLAAPAHALSDRLEAFIGSEPPLFA
                     TADEGVAAFKAAMATGDIVQISKLLGLDPDKVKAAEGIADRVGEMKQAAAKLVTVEGE
                     GDERIISIGSEVWPFPFPLVKSEKDGKWAFDTVAGIEEIVNRRIGENELHAIETARTY
                     VEAQRDYAAEDRDADGVLEYAQKLISTEGQTDGLYWPIEQGDGVSPVGPNIDLAALDK
                     AAKGDGYFGYKFRILRGQGNQVAGGRYDYVINGNMIGGFGLIAWPARYAETGVSTFVI
                     SHAGILYEKDLGPDTEKLAGEIRRFNPDSSWELVND"
     gene            1657..2343
                     /gene="aqpZ"
                     /locus_tag="AC16A0_04725"
     CDS             1657..2343
                     /gene="aqpZ"
                     /locus_tag="AC16A0_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HIR3104668.1"
                     /translation="MKKLAAEVFGTFWLVLGGCGAAVLATGYPDVGIGFLGVALAFGL
                     TVLTMAYAVGHLSGGHFNPAVTVGLAVAGRFNWGSVPGYVVAQLVGSVLAAAVLYLIA
                     TGKADAVIGGFASNGYGEHSPGKYSLFAALITEVVLTAFFLIVILGATSKRAPAGFAP
                     IAIGLALTLIHLVSVPVTNASVNPARSTGVALFAEGGWALQQLWLFWLAPLAGAAIGA
                     VIYQALLAED"
     gene            2503..>2736
                     /locus_tag="AC16A0_04730"
     CDS             2503..>2736
                     /locus_tag="AC16A0_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104669.1"
                     /translation="MTMRLDRRAFSIGALSLGLTGCAGATVEELRDPVPEPLLSQAAL
                     PGYKNIRYWGDDAASIDPAVLQEIAAQQARGLKS"
BASE COUNT          539 a          830 c          897 g          470 t
ORIGIN      
        1 aagctcgaca tcaacaatgt cgactggaag aacgtcgatc gcagcaagat caagtttgac
       61 aagaaccagc tcaacaagat cgaccggtcg aaggtcaaga acaacgtcaa gaacaacgac
      121 cgcaacaaga tcaccaacaa ggcgggcgac ctcaagaaga accggccgac gaccctgccc
      181 ggcaagggca accggccgac caaggacgtg cgcgccagca cgctcgaggg cctcaagaag
      241 cagcccggcg cgaggccggc ggacaagatg gcacggccgg ggcagggggc cggagacaag
      301 atggccaggc ccggacaggg tgtgaccaac aagcgtcccg aaggcggcaa gatcgaccgc
      361 ccggtgggca agcccaagcc cgcggcgcgt ccggacaacc gcccgaagac tccctcgccg
      421 atgggcgaca tgaagcgcgg caagaatgcc aagtttgatt ccaaccgcgg tgccaagtcc
      481 atgggcggcg gaggctacaa gcggccgagt ggcggcggcg gcggccagaa gatgaagaag
      541 cggcccatgc ccaagggcgg tggcggcggt cgcggcggcg gcgggcgcgg ccggcgttga
      601 gcagagcatc gggaggacag gacatgacca tgaccatcaa gcatttgatt tcctctgcct
      661 tgctcggcac ggcgctcgtc ttcgggaccg tttcccttgc agcgcccgcc catgcgctgt
      721 cggatcggct ggaagccttc atcggttccg agccgccgct tttcgccacg gccgacgagg
      781 gcgttgccgc cttcaaggcg gcgatggcta ctggcgacat cgtgcagatc tcgaagctct
      841 tgggactcga tcccgacaag gttaaggccg ccgagggcat cgctgaccgg gtcggagaga
      901 tgaagcaggc tgccgccaag ttggtgaccg tcgaaggcga aggtgacgag cgcatcatca
      961 gcatcggttc ggaggtctgg ccattcccgt tcccgctggt caagagcgag aaggacggaa
     1021 agtgggcctt cgacacggtg gccggcatcg aggaaatcgt gaaccgccgt atcggcgaaa
     1081 acgagctcca cgcgatcgag accgcccgga cctatgtcga agcccagcgc gactacgcgg
     1141 cggaagaccg cgatgcagac ggcgtgctcg aatatgccca gaagctgatc agcaccgagg
     1201 gccagaccga tggcctctac tggccgatcg aacagggtga cggcgtcagc ccggtgggtc
     1261 ccaacatcga tctggccgcg ctcgacaagg cggcgaaggg cgacggctat ttcggctaca
     1321 agttccgcat cctccgcggg cagggcaacc aggtggccgg cgggcgctat gactatgtca
     1381 tcaacggcaa catgatcggc ggcttcggtc tcatcgcctg gccggcgcgc tatgccgaga
     1441 ccggcgtaag caccttcgtg atcagtcacg caggtatcct ctatgaaaag gacctgggac
     1501 cggataccga gaagctggcc ggtgagattc gccgtttcaa ccccgacagc agttgggaac
     1561 ttgtgaacga ctaagcaagg gcgaccaccg gctgtcggtc tgcgctgatc gaggtgcggc
     1621 ccggccaaca ttggtcaatc gaagtgggga atggagatga agaaactagc agccgaggtg
     1681 ttcggcacgt tctggcttgt ccttggcggc tgtggcgccg cggtgctggc cacgggttat
     1741 cctgatgtgg gaatcgggtt tctgggagtt gctcttgcct ttggtctgac cgtcctgacg
     1801 atggcctatg cggttggcca tctctcgggc ggccatttca atccagccgt tacggtgggc
     1861 cttgcggtcg cagggcgctt caactggggc agcgtacccg gctatgtcgt cgcgcagctg
     1921 gtaggctcgg tcctcgccgc cgccgtgctt taccttatcg ccactggcaa ggccgatgct
     1981 gtgattggcg gttttgcttc caacggatat ggcgaacatt cacccgggaa gtactcgctc
     2041 tttgcggccc tgatcacgga agtggtgctg acggcattct ttttgatcgt catcctgggg
     2101 gccaccagca agcgggcgcc ggccgggttc gcgccgattg ccattggcct cgcactgacg
     2161 ctcatccacc ttgtttccgt cccggtgacg aatgcgtcgg tcaacccagc ccggtccaca
     2221 ggcgttgcct tgtttgccga gggcggctgg gcgctgcagc agctctggct cttctggctc
     2281 gcgccgcttg cgggcgctgc cattggggcc gtgatctatc aggctctcct tgcggaagac
     2341 tgatcgtccg gcgcaactgc aggcaagacc atgaagcagg gtaccgcatt tgcggtccct
     2401 gcttcatgaa tgctacaagc agccgataga gttaagaatt aactaatttg catcaaactc
     2461 gactggaatc cgatgcttgc gtgtaggccg tagacactcg acatgaccat gagactagac
     2521 cgccgcgcat tttccatcgg tgccctgtcc ctcggactga ccgggtgcgc cggcgcgacc
     2581 gtcgaagaac tgcgcgatcc ggtgccggag cccttgctca gccaggcggc actgccgggt
     2641 tacaagaaca tcagatactg gggcgacgat gccgcctcca tcgatccggc cgtgctgcag
     2701 gagattgccg cgcagcaggc tcgcggcctc aagtcg
//
LOCUS       DBNGVR010000215         2579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3614681, whole genome shotgun sequence.
ACCESSION   DBNGVR010000215 DBNGVR010000000
VERSION     DBNGVR010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3614681"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="AC16A0_04735"
     CDS             <1..1494
                     /locus_tag="AC16A0_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3104670.1"
                     /translation="PTDAQSIFEEGIRIPPTKIIKKDVLQEDILEIILHNCRMPQWNR
                     SDFYAIVAAIRTAEKRVIEMSNRFGDDTFYSALEDLLDRNKRAMSKLINAAVPTKKQY
                     FEDYLCDDGMGMGPYKIKCAMWREGDKVIFDFEGTDPQSTGSVNFYLNEEMFKMFCGV
                     YMIMVFDPQIMFNDGFYDLMEVRIPEGSLLKPRFPAALSCRTHALGRIFDILGGLLGQ
                     GNPDFLCAAGFSDSPHFMYSGHRKTGEWYQLYSIGFGGVPGKPFGDGPDGHSLWPSFT
                     NVPNEFLESYFPLRIEAYETLADTGGAGLHRGGNALRVGYRFLEPGQISIHDDRWLTY
                     PWGVNGGLPGARSRKIMIRKNGKKELLPSKCDRVPVAEGDLLYFDTWGGGGWGDPLKR
                     PAEKVAFDVEAGLVSREGAKRYGVVIKPNWTIDEKATTALRAKMAKKRGKPKLFDFGG
                     TIPELKKACKKETGLEAPKTPVFQTWAKPGADGAKAGSGKKAARTVA"
     gene            complement(1583..2410)
                     /locus_tag="AC16A0_04740"
     CDS             complement(1583..2410)
                     /locus_tag="AC16A0_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3104671.1"
                     /translation="MTSTRPIRALLRGLEVLHVLNQQNGATVSEVAMAIDLPRTTTYR
                     ILETLCHAGYAYRAASDERYRLTILVRGLSDGFDDEAWVTQIARPYMYELCNDLVWPI
                     AIATLSGSTMLVRQTTDHRSPLAVEKRGPGFRVAILESASGIAYLTWCPKEQRDALLD
                     LLAKSKRTEDQPAANRKKVNDLLLETRKRGYAAWRRPRRVSDELSLSVPILAGERLLA
                     SLTIRFSSTAVPETEAIQRFIPRLKATAQRIGEVFLSQSEQQALSIATDNPAPASEE"
BASE COUNT          493 a          805 c          823 g          458 t
ORIGIN      
        1 ccgaccgacg cccagtcgat cttcgaggag ggaattcgca tcccgccgac caagatcatc
       61 aagaaggacg tcctgcagga ggacatcctc gaaatcatcc tccacaactg ccggatgccc
      121 cagtggaacc ggagcgactt ctacgccatc gttgccgcca tccggaccgc cgagaagcgg
      181 gtcatcgaga tgtcgaaccg attcggtgac gacaccttct attccgcact cgaggacctt
      241 ctcgaccgca acaagcgggc catgagcaag ctcatcaacg cggctgtccc caccaagaag
      301 cagtacttcg aggactacct gtgcgatgac ggcatgggca tgggcccgta caagatcaag
      361 tgcgccatgt ggcgcgaagg tgacaaggtc atcttcgact tcgaaggcac cgacccccag
      421 tcgacgggct ccgtgaactt ctacctcaac gaggagatgt tcaagatgtt ctgtggcgtg
      481 tacatgatca tggtcttcga cccgcagatc atgttcaacg acggcttcta cgacctcatg
      541 gaggtgcgca ttccggaggg ctcactgctg aagccccgct tccctgcagc actctcgtgc
      601 cgtacgcatg ccctcgggcg catcttcgac atcctgggcg gactgctcgg ccagggaaac
      661 cctgacttcc tgtgcgccgc cggcttctcc gacagccccc acttcatgta ctcgggccac
      721 cgcaagacgg gcgagtggta ccagttgtac agcatcggct tcggtggcgt tcccggtaag
      781 ccgtttggag acggtccgga cggacactcc ctctggccca gcttcaccaa cgtccccaac
      841 gagttcctcg agagctattt cccgctgcgg atcgaggcct acgagaccct ggccgatacc
      901 ggtggggctg gcctgcatcg cggcggcaat gcgctgcgcg tgggctatcg cttccttgag
      961 cccggccaga tctcgatcca cgacgatcgc tggctgacct atccctgggg cgtcaatggc
     1021 ggcctgccgg gggctcgaag ccgcaagatc atgatccgca agaatggcaa gaaggagctg
     1081 ctgccgtcca agtgcgaccg tgtcccggtg gccgagggcg acctgctgta cttcgacacc
     1141 tggggcggcg gcggctgggg cgatcccctg aagcggcccg ccgagaaggt ggcgttcgac
     1201 gtggaagccg ggcttgtctc ccgcgaaggc gccaagcgct atggtgtcgt catcaagccg
     1261 aattggacca tcgacgagaa ggcaacgacc gccctgcgcg cgaagatggc caagaagcgt
     1321 ggcaagccca agctcttcga cttcgggggg accattcccg agctcaagaa ggcgtgcaag
     1381 aaggaaaccg gtctcgaggc gcccaagacc ccggtattcc agacctgggc aaagcccggt
     1441 gccgacggcg caaaggctgg ttccggcaag aaggcggccc gcacggtagc ctgacgcgca
     1501 ccggccagac gacacgtgcc aacacaaggc cctggttcac cgcgcgtgtg ccagggcctt
     1561 cgtgcttgtc gtccgggctg gcctattcct ccgatgccgg agcggggttg tccgtggcga
     1621 tcgagagcgc ctgctgctcg ctctgggaaa gaaagacctc gccaatgcgc tgggcggttg
     1681 ccttgagccg gggaatgaac cgctggatgg cctcggtttc cggcaccgcc gtgctcgaga
     1741 accggatcgt cagggaggcg aggaggcgct caccggccag aatcggtacg gagaggctga
     1801 gttcgtcgga cacgcgcctt ggccggcgcc acgccgcata gccgcgcttg cgggtttcaa
     1861 ggaggagatc attgaccttc ttgcgatttg ccgccggctg gtcttccgtc cgcttggact
     1921 tcgccagcag atcgaggagc gcgtcgcgct gttccttcgg gcaccaggtc aggtacgcga
     1981 tgcccgaggc tgactccaga atggccacac ggaaaccagg gccgcgcttc tcgacagcca
     2041 gcgggctccg gtggtccgtc gtctgccgga cgagcatcgt ggatccggac agcgttgcaa
     2101 tggcgatggg ccacacgaga tcgttgcaga gttcgtacat gtaggggcgg gctatctgcg
     2161 tgacccaggc ctcgtcgtca aaaccgtctg acaggccacg aaccaggatc gtcaggcgat
     2221 agcgctcgtc actggcggcg cgatacgcat agcctgcgtg gcagagcgtt tccaggatgc
     2281 gataggtggt ggtgcgaggc agatcgatgg ccatcgcaac ttcggacacg gttgccccat
     2341 tttgctggtt aaggacgtgc aggacctcga gaccgcgaag cagcgcgcga atggggcggg
     2401 tggaagtcat ctggtggagc atcctgacct gaccgacagg cggacgatgt tagtcaacgt
     2461 ccccggcaaa tccaaggccg gttttggatt tgttacacac cgttacgaaa tggaaatcgt
     2521 cccttggtca cgcccggcgg tcaatgctcc agagttccgc tgggacggtt agcatggcc
//
LOCUS       DBNGVR010000216         3315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1062554, whole genome shotgun sequence.
ACCESSION   DBNGVR010000216 DBNGVR010000000
VERSION     DBNGVR010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1062554"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC16A0_04745"
     CDS             complement(<1..261)
                     /locus_tag="AC16A0_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HIR3104672.1"
                     /translation="MSPLTSAYVLPIILLLASNVFMTFAWYGHLNYTSKPLWMAVMVS
                     WGIAFFEYWRAVPANRIGYQVYSAAELKTLQEVITFAVFAVFS"
     gene            complement(333..1217)
                     /locus_tag="AC16A0_04750"
     CDS             complement(333..1217)
                     /locus_tag="AC16A0_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104673.1"
                     /translation="MKRYAPLLLLAAAAPALAASETLLSFDDPAVYFASALGRQVNIR
                     FTDSFVASHLPQADYDGVILSGLSGDKVCFFGGEKGLDPADPKLADVARVEGNDICVP
                     RNVVAVKAGKPAVDGQPPEPFYATDQALCSWNWQRGGSVGLWTEDCKFESGRWNIAYD
                     KEKDLFGLHVDNGELYPVLRHFRTDPAKGPEGLLPDLKARGLVLDSPECVFEKNEEQF
                     GAPGWTIWQVVPTGKIKEAFDAQVKLEVPPPPCGEVGYAADFIGFFMVHKDHPDRMVF
                     VNLGQDGTMIDPFSLTLF"
     gene            1289..2485
                     /locus_tag="AC16A0_04755"
     CDS             1289..2485
                     /locus_tag="AC16A0_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR3104674.1"
                     /translation="MNDRTRVLPPFAILVAISAIGPLSLNIFIPSMPGLQDEFGIPYG
                     KAQLTLTLFLIGMAVCQLIYGPISDRVGRRPMLLGGMAVFVLASVLAALAPSIEVLIG
                     ARLLQALGGAAGIVLARAMVRDVFDRDKSASVISYITMAFVVAPMVAPVLGGLIEAIA
                     SWRYDFWLLAVLGAAVLAAAWLRLPETLVRREGAPNAVGLVAGAVHLAGIARFRSYTL
                     TLAFTSAVFFAFLGGAPHIMVDVLHRTPMEYGMWFVLISSGYMLGNFLSGRFTQRMGI
                     DRMMLMGCGVTTLGGLLCLAAAALGFLSPPTLFVPMAFAALGNGLTIPNGTAGAISVD
                     ARLTGAAAGWAGFVQMACGAAASQLVGTLQEDFPFSVFWCMSAASILALAIHLGALRR
                     KRLATS"
     gene            2609..3016
                     /locus_tag="AC16A0_04760"
     CDS             2609..3016
                     /locus_tag="AC16A0_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104675.1"
                     /translation="MKLTNRGLGRLAAVSAVALTLAACASTGTDNVANNSPGPEVMSA
                     QEIRGVLAGKSWKFRGPNNTGTTLYAEDGTSLVEVDGKGTTRGKWMTKDGQLCESFDP
                     APFLPGGVPMSCYPFSGSGGVYQAGKATFTLAS"
     gene            3117..>3315
                     /locus_tag="AC16A0_04765"
     CDS             3117..>3315
                     /locus_tag="AC16A0_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104676.1"
                     /translation="MTAAITRRSLLLSGLAFLGSGLAAGAKANALYGGEDQEYFNPDL
                     LPPAESEIDYPIEPVNPRRIRK"
BASE COUNT          577 a         1065 c         1043 g          630 t
ORIGIN      
        1 cgaaaacacc gcaaaaaccg caaaggtaat cacctcctgg agggtcttga gctcagccgc
       61 cgaatagacc tgatagccga tccggttcgc cggcaccgcc cgccagtatt cgaagaaggc
      121 gatgccccag ctcaccatca ccgccatcca tagcggcttg ctggtgtagt tgaggtggcc
      181 gtaccaggcg aaggtcatga agacattcga ggccagcaag agaatgatgg gaaggacata
      241 ggccgaggta agcggggaca tgcgcgcgat tccgaagggc gtgacgcctg gtgcatgaac
      301 gccgccgctg acgtcgtcaa ggcggcgaaa gctcagaaca gggtcagcga aaacggatcg
      361 atcatcgtgc cgtcctggcc gagattcacg aagaccatac ggtctggatg gtccttgtgc
      421 accatgaaga agccgatgaa atccgctgca tagcccacct ctccgcaagg cggtggcgga
      481 acctcgagct tgacctgcgc atcgaaggct tccttgatct tgccggtggg cacgacctgc
      541 cagatggtcc agccgggggc gccgaactgc tcttcgttct tctcgaaaac gcattcgggc
      601 gaatccagca ccaggccgcg ggccttgagg tcgggcagca ggccctcggg acccttggcc
      661 ggatcggtgc ggaaatggcg caacaccggg tagagctcgc cattgtcgac atggaggccg
      721 aacaggtcct tctccttgtc ataggcaatg ttccatcgcc cggactcgaa cttgcagtcc
      781 tcggtccaca ggcccacgct gccacccctc tgccagttcc agctgcacaa ggcctgatcg
      841 gtcgcataga agggttccgg cggctggcca tcgacggccg gctttccggc cttcaccgcc
      901 acgacattgc gcgggacgca aatgtcgttg ccttccacgc gggcaacatc ggctaacttg
      961 gggtcggccg gatccagtcc cttttcaccg ccgaagaaac agaccttgtc gcccgaaagg
     1021 ccgctgagaa tgacgccgtc gtaatcggcc tgcggcaggt ggcttgcgac gaagctgtcg
     1081 gtgaagcgga tgttgacctg acggcccagg gccgatgcga aatacacagc gggatcatcg
     1141 aaggacagca gggtctcgga ggcggccagg gccggtgcgg cggcagccag cagcaggagt
     1201 ggggcatagc gcttcatgcg ccaagctcat gccagacttc gcgcggcttg gctatcggga
     1261 aggccgtgag ccgtctatga gacgggccat gaacgatcga acccgggtgc tgccgccatt
     1321 cgcgatcctc gtcgcgatca gcgccatcgg ccccctgtcg ctgaatatct tcattccctc
     1381 catgccgggg ctgcaggacg agttcggcat tccctatggc aaggcccagc tcacgctgac
     1441 cctgttcctg atcggcatgg cggtctgcca gctgatctac gggccgatct cggaccgggt
     1501 gggccgccgt cccatgctgc tgggcggtat ggcggttttc gtactggcaa gcgtgctggc
     1561 agccctggcg ccgtcgatcg aggtgctgat cggcgcccgg ctgctccagg cgctgggtgg
     1621 cgccgccggc atcgtgctgg cccgggccat ggtgcgcgac gtcttcgacc gcgacaaatc
     1681 ggccagcgtc atctcctaca tcaccatggc cttcgtggtg gcgcccatgg tggctccggt
     1741 gctgggcggt ttgatcgagg ccattgccag ctggcgctat gatttctggc tgctggccgt
     1801 attgggcgcg gcggtgctgg cggcggcttg gctgcgcctg ccggagaccc tggtccgccg
     1861 cgagggcgcg cccaacgccg tgggacttgt ggccggcgcc gtgcatcttg cgggcatcgc
     1921 acggttccgc agctacacgc tgaccctggc cttcacttca gcggtcttct tcgccttcct
     1981 gggcggcgcg ccccacatca tggtcgacgt gctgcacaga accccgatgg aatatggcat
     2041 gtggttcgtg ctgatctcct cgggctacat gctgggcaat ttcctgtcag gccgctttac
     2101 ccagcgcatg ggcatcgacc gcatgatgct gatgggctgc ggcgtgacga cactgggcgg
     2161 gctcctgtgc ctggcggcgg ccgcgctcgg gtttctttcg ccccccaccc tcttcgtccc
     2221 catggccttt gcggccctcg gcaatggcct gaccattccc aacgggaccg cgggggcgat
     2281 cagcgtcgat gcccgcctca ccggcgccgc cgccggctgg gccggcttcg tgcagatggc
     2341 ctgcggtgcc gcggcctcgc aacttgtcgg cacgctgcag gaagatttcc ccttttccgt
     2401 gttctggtgc atgtcggcgg ccagcatcct ggccctcgcc atccatcttg gcgcgctgcg
     2461 ccgcaagcgc ctcgcaactt cgtgagtcgc ggcccaaaat tcgcccgttt tcgggacttt
     2521 agctcgaaaa tcattcgtga tggctttagc agcaaaatcg aatctgctag agttgacctt
     2581 cggggagtca atgaggagtt tcctgccgat gaagctgacc aatcgcggtc ttggccgtct
     2641 tgccgctgta tccgcggtgg cattgacgct ggccgcctgt gcctcgaccg gcacggacaa
     2701 tgtcgccaac aacagccccg gtcccgaagt catgtcggct caggagatcc gcggtgtgct
     2761 tgccgggaaa agctggaagt tccgcggccc caacaacacc ggcaccacgc tctatgctga
     2821 ggacggcaca tccctggtag aagttgatgg caagggcacg acccgcggca agtggatgac
     2881 caaggacggc cagctctgcg agagcttcga tccagcgccc ttcctgccag gtggcgtacc
     2941 gatgagctgc tatcccttct cggggagcgg tggcgtctat caggccggta aggccacttt
     3001 cacgcttgcg tcatgatgat ttgaacactt gccacaattg cgccacgctc gctcctaact
     3061 cttgcctgcg gcgaggtgca tcgccaaccc acaacatgtc gaaacgggat gatcggatga
     3121 ctgcggcaat cacccgccga agcctgttgc tttcaggact tgcgttcctt gggagcggcc
     3181 tggcggctgg cgccaaggcg aacgcgctct atggcggcga ggatcaggag tacttcaatc
     3241 ccgatctgct gccaccggcc gaatcggaaa tcgactatcc catcgagccc gtcaaccctc
     3301 gccgcatccg caaga
//
LOCUS       DBNGVR010000217         5071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4203625, whole genome shotgun sequence.
ACCESSION   DBNGVR010000217 DBNGVR010000000
VERSION     DBNGVR010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4203625"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="AC16A0_04770"
     CDS             <1..432
                     /locus_tag="AC16A0_04770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3104677.1"
                     /translation="VEQVPVGPGKTSLKKGELVESIQLPARKPRSGDAYLRFIPRSEM
                     DIAVVSAGVNLSLDAKGVVTDARVSLGAVAPTVLLVPAAAKALIGSKLDDAALEKLGA
                     AASAACRPIDDKRGTIEFRTKVAAVLAKRAAKIAYARAGGK"
     gene            435..908
                     /locus_tag="AC16A0_04775"
     CDS             435..908
                     /locus_tag="AC16A0_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3104678.1"
                     /translation="MALLVSTTINGDHVEFACEPDETLLDALRNRVGLTGVKEGCGTG
                     DCGACSILIDGRLVCSCLVLGAEAEGSEIETVEGMADGATLHPLQQKFLEHAALQCGV
                     CTPGFLIAAKALLEKNPDPSEEEIRFGLAGNLCRCTGYDKIVRAVQDAAKEMRGA"
     gene            912..3176
                     /locus_tag="AC16A0_04780"
     CDS             912..3176
                     /locus_tag="AC16A0_04780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3104679.1"
                     /translation="MPLDSNYTGQSFKSVGTRPLRPDGIDKVTGRAKYGADHNMPGQL
                     VGKILRSPHAHAKIKKIDTSRAEKLAGVKAVITAADLPDLTNGDSGMFDILDNCLART
                     KVFYDGHAVAAVAAVDAKTAREALKLIKVDYEVLPHVTEVDEAVKPKAPILHANCFTE
                     GVDPKPTKPSNIAKVTEFGHGDVDAGFAEADFVIERNFKTEQTHQGYIEPHACVASVS
                     PDGTGEHWVCTQGHFVYRNHCAMLLGMDVSKLRVTSSEIGGGFGGKTHVWAEPVALAL
                     SRKANRPVKLVMTRDEVFRASGPTSATSIDVKIGIKKDGTITAGSAELRYASGPYLSM
                     WAELGAMTSFACYDLKNVRTRGYEVLVNRPKSAAYRAPSAPMAAFAVESVVDELAHKI
                     GMDALDVRIKNAAKQGTKSSYGPVYGPIGIGPTLEAARKHPHMSAPLGPNQGRGMACG
                     FWFNFGGQTCTDLNVGQDGSVTLTVGTVDVGGSRASLALCVAEELGIPYSQVKCLVAD
                     TGTLGHNDMTDGSRGTFSSSMAGIFAARNAIGVLKERAAKMWEIPVEDVEWEGGHAKA
                     VGEKYGNLAPLSLKEIAAAAANTGGPVAGHAEIVADGAGVSFATHICDVEVDPETGRS
                     RIMRYTVIQDAGKAVHPTYVEGQYQGGAAQGIGWALNEEYVYGKDGRLQNAGFLDYRI
                     PVCSDLPMIDTQILEIPNPNHPYGVRGVGETSIVPPIAAIANAMSNAFGVRMTHVPMS
                     PPRVLKALEAARKA"
     gene            3178..3423
                     /locus_tag="AC16A0_04785"
     CDS             3178..3423
                     /locus_tag="AC16A0_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3104680.1"
                     /translation="MAEIHLWGALRPAAGGAPLIHVEAKTIRELFRKLEEDYPGMAPH
                     IARGVAVSINGKIFRDSWQEPLPEGAEIYLLPRIQGG"
     gene            3554..4474
                     /locus_tag="AC16A0_04790"
     CDS             3554..4474
                     /locus_tag="AC16A0_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HIR3104681.1"
                     /translation="MQLKGFHHLTAITADAPGNHKFYTKILGLRLVKKTVNQDDTSAY
                     HLFYADGVGSPGTDITFFDWPVGREHRGTHSITCTGFRVADSKALTYWKHRFTMQGVR
                     HGDVVERDGRPTLDFEDAEGQRLSLVADDSGEAHAWSESPVPAEHQIRGLGPITISVP
                     DLKPTDMVLTRLMNMTPARRFGDTHVYTMGGDGAVAELHVAVEPRLPPAQQGAGGVHH
                     VAFNIPSTEYQVWADRLSSMRVPNSGPVDRFWFKSLYFREPNGILFELATDAPGFATD
                     ESMDRLGTTLSLPPFLEPRRAQIEAGLKPL"
     gene            complement(4489..>5071)
                     /locus_tag="AC16A0_04795"
     CDS             complement(4489..>5071)
                     /locus_tag="AC16A0_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3104682.1"
                     /translation="DILRSDVENEDELHWLEPAGWRTMQILRPDGGDCAVGEEGELAV
                     RLTDIDCGAYLGDPATSASVFRDGRFHPGDMAIRREDGRIRILGRVSEVLNVAGRKIA
                     VGPVEERLQRILGGRPVCVFAQLDSAGNDEVVVAVETGSVPSDAQMREVANLLRGFHK
                     IKVTVVSRFPRTATAMAKIDRQSLRRLLNERPA"
BASE COUNT          950 a         1706 c         1582 g          833 t
ORIGIN      
        1 gtcgagcagg ttccggtcgg accgggcaag acctcgctga agaagggcga actggtcgag
       61 tcgattcaac ttcccgcgcg caagccgcgt tcgggcgacg cctacctgcg cttcattccg
      121 cgcagcgaaa tggacattgc ggtggtcagc gcaggcgtca atctttcgct tgatgccaag
      181 ggcgtggtga ccgacgcgcg cgtgtcgctc ggggccgtcg ctcccaccgt cctgctggtt
      241 ccggctgctg cgaaggcgct gatcggcagc aagctcgatg acgccgcgtt ggagaagctc
      301 ggcgccgccg catccgccgc ctgccgcccg atcgacgaca agcgcggtac catcgaattc
      361 agaaccaagg tcgccgccgt gctggcgaag cgcgccgcca agatcgccta tgcgcgcgca
      421 ggaggtaagt gattatggcc ctactcgtat cgacaaccat caatggcgac cacgtggaat
      481 tcgcctgcga gcctgatgag acccttctcg atgccctgcg caatcgcgtg ggattgaccg
      541 gcgtcaagga aggctgcggc accggcgact gcggcgcctg cagcatcctg atcgatggca
      601 ggcttgtctg ctcctgcctg gtgctcggcg ccgaagccga gggcagcgag atcgagaccg
      661 tggaaggcat ggccgatggc gcaacgctgc acccgctgca gcagaagttc ctggaacacg
      721 cggcactgca gtgcggcgtc tgcaccccgg gcttcctgat cgccgccaag gccctcctcg
      781 agaagaaccc cgatccgagc gaggaggaaa tccgtttcgg actcgccggc aacctttgcc
      841 gttgcaccgg ctatgacaag atcgtgcgcg ccgtgcagga cgccgccaaa gagatgagag
      901 gagcctgatc catgccgctc gacagcaact ataccggcca gtcgttcaag tccgtcggca
      961 cgcgccccct gcgtcccgac gggatcgaca aggtgacggg ccgcgccaag tatggcgccg
     1021 accacaacat gccgggccag ctcgtgggca agatcctgcg cagcccccat gcacacgcca
     1081 agatcaagaa gatcgataca tcccgcgccg agaagctggc cggcgtcaag gcggtgatta
     1141 ccgccgccga cctgccggac ctcaccaacg gcgactccgg catgttcgac atcctcgaca
     1201 actgcttggc ccgcaccaag gtcttctatg acggccacgc cgttgccgcc gttgcggcag
     1261 tcgacgccaa gacggccagg gaagccctga agctgatcaa ggtcgactac gaagtccttc
     1321 cgcatgtgac cgaggtcgac gaggcggtga agcccaaggc gccgatcctg cacgccaact
     1381 gctttaccga aggcgtcgat cccaagccga ccaagccatc caacatcgcc aaggtcaccg
     1441 agttcggcca tggcgacgtc gacgccggct ttgcggaggc cgacttcgtc atcgagcgca
     1501 acttcaagac cgagcagacg caccagggct atatcgagcc gcatgcctgc gtggcgagcg
     1561 tcagccccga cggcactggc gaacactggg tctgcaccca gggccacttc gtctatcgca
     1621 atcactgcgc catgctgctg ggcatggatg tgtcaaagct gcgcgtgacc tcttcggaga
     1681 tcggcggtgg tttcggcggc aagacccatg tctgggccga gcctgtggca ctggcgttgt
     1741 cgcgcaaggc caatcgcccg gtcaagctgg tgatgacgcg cgacgaggtg ttccgcgctt
     1801 cgggaccgac cagcgccacc tcgatcgacg tgaagatcgg catcaagaag gacggaacca
     1861 tcacggctgg ctcggcggaa ctccgctatg cctcgggtcc ctatctcagc atgtgggctg
     1921 aacttggcgc gatgacgtcc tttgcatgtt acgatctgaa gaatgtccgc acccgcggct
     1981 acgaggtgct ggtgaaccgc ccgaagtcgg cggcctatcg tgcgccctcg gcgcccatgg
     2041 cggccttcgc cgtcgaaagc gtcgttgacg aactcgccca caagatcggc atggatgcgc
     2101 tcgatgtccg catcaagaac gcagccaagc agggcaccaa atcttcctat ggtccggtct
     2161 atggtccgat cggaatcgga ccgacgctgg aggcggccag gaagcatccg cacatgtcgg
     2221 cgccgcttgg ccccaaccag ggccgcggca tggcctgcgg attctggttc aactttggcg
     2281 gccagacctg caccgacctc aacgtcggcc aggacggttc ggtgacgctg acggtgggaa
     2341 cggtggacgt cggcggctcg cgcgcctcgc tggcgctttg cgtcgccgag gaactgggca
     2401 ttccctacag ccaggtgaag tgcctggtcg ccgacaccgg aacgctcggt cacaacgaca
     2461 tgaccgacgg tagccgtggc accttctcct cgtccatggc cggcatcttc gcagcccgca
     2521 acgccattgg cgtgctgaag gagcgcgccg ccaagatgtg ggaaatcccc gtcgaggacg
     2581 tggagtggga aggcggacat gccaaggcgg taggcgagaa gtacggcaac ctcgcgccgc
     2641 tctcgctcaa ggagatcgcc gcagccgccg ccaatactgg cgggccggtg gccggccatg
     2701 ccgagatcgt cgcggacggt gccggcgtgt ccttcgccac ccatatctgc gacgtggaag
     2761 tcgatccgga aaccggccgc tcgcgcatca tgcgctatac ggtcatccag gacgccggca
     2821 aggcggttca ccccacctat gtcgagggcc agtatcaggg cggcgccgcc cagggcatcg
     2881 gctgggcgct gaacgaggaa tatgtgtatg gcaaggacgg tcggctgcag aacgccggct
     2941 tcctcgacta ccgtatcccg gtgtgttcgg acctcccgat gatcgacacc cagatcctgg
     3001 agatccccaa ccccaaccac ccctatggcg tgcgcggcgt tggcgagacc tcgatcgtgc
     3061 cgcccatcgc cgccatcgcc aatgccatgt cgaacgcgtt cggcgtccgc atgacccatg
     3121 tgccgatgtc gccgccgcgc gtgctcaagg cgctcgaggc agcacggaag gcctgatttg
     3181 gccgagatcc acctgtgggg ggcgctccgt ccggcagcgg gcggagcgcc cctcattcat
     3241 gtcgaggcca agaccatccg cgagcttttc cgcaaactgg aagaggacta ccccggcatg
     3301 gcgccgcata tcgcgcgcgg tgtggcggtc tcgatcaacg gcaagatttt ccgcgacagc
     3361 tggcaggaac ccctccccga gggcgccgaa atctatctcc tgccgcgcat tcagggcggc
     3421 tgatcaccgg ggctggagtg ccgccggcaa tgcttgcatt ccggaatccg cggtccgata
     3481 tgtgcgaggc cggagcgcac gaaggctccc caacatgatc gccgccacgc tctggcgcgc
     3541 cggaacctga cacatgcagc tcaagggctt tcaccacctg acggcgatca cagccgatgc
     3601 cccgggaaac cacaagttct acaccaagat tctcggcctt cgtctggtga agaaaacggt
     3661 gaaccaggac gatacctcgg cctaccacct cttctatgcg gatggcgtag gttctccggg
     3721 aaccgacatt accttcttcg attggccggt cggccgcgag catcgcggca cccattccat
     3781 cacctgcacc ggattccgcg tggctgactc caaggcgctg acctactgga agcaccgttt
     3841 cacgatgcag ggtgtgcggc acggtgacgt ggtcgagcgc gatggccgtc caacgctgga
     3901 cttcgaagac gcggaaggcc agcgcctcag cctcgtggca gacgacagcg gcgaggcaca
     3961 tgcctggtcc gagagcccgg ttccggccga gcaccagatt cgcggcctcg gccccatcac
     4021 catcagcgtt ccggatctca agcccaccga catggtgttg accaggctga tgaacatgac
     4081 gccggcccgg cgcttcggcg acacccatgt ctataccatg ggcggcgatg gagccgtggc
     4141 cgaacttcac gtcgccgtgg agccgaggct gccgcctgcc cagcagggcg ccggcggcgt
     4201 acatcacgtc gcgttcaaca taccgtccac ggagtatcag gtctgggccg accggcttag
     4261 ctccatgcgc gttcccaaca gtggcccggt ggaccgcttc tggttcaaga gcctctactt
     4321 ccgcgaaccc aacggcatac tcttcgaact ggccaccgac gcgccaggat ttgccactga
     4381 cgagtcgatg gacagactgg gcaccacact ctcactgccg cccttcctcg agccgcgacg
     4441 cgcacagatc gaggcggggc tgaagccgct atagaccgcg cgccttgctc aggccggccg
     4501 ctcgttcagg agccgtcgca aggactgacg gtcgatcttg gccatggccg tagccgtgcg
     4561 cggaaagcgc gacacgaccg tgacttttat cttgtgaaag ccgcgcaaga gattcgcgac
     4621 ttcgcgcatc tgtgcgtccg agggtacact cccggtctcg actgcgacga cgacttcatc
     4681 attgccggcg gagtccagct gagcaaagac gcagaccggc cgtccgccga gaatgcgctg
     4741 gaggcgctcc tcgaccggac ctacggcaat cttcctgccg gcaacattca gcacctcgct
     4801 cacgcgcccg agaatgcgga tgcggccatc ctcccgcctg atggccatgt ctcccggatg
     4861 gaagcgaccg tcgcgaaaca cggatgccga cgtggcaggg tcaccaagat aggcgccaca
     4921 gtcgatatcg gtcaggcgaa ctgcaagctc gccctcctca ccgacggcgc agtccccgcc
     4981 atcgggcctc aggatctgca tggtccgcca gccggcgggc tccagccaat gcaattcatc
     5041 ctcgttctcg acatcggagc gcaggatgtc g
//
LOCUS       DBNGVR010000218         6928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3812624, whole genome shotgun sequence.
ACCESSION   DBNGVR010000218 DBNGVR010000000
VERSION     DBNGVR010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3812624"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="AC16A0_04800"
     CDS             complement(<1..1162)
                     /locus_tag="AC16A0_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3104683.1"
                     /translation="MTKAEGGAQFIVANGNLYHLFEEGFAQQGARVAIRPADGQSTMT
                     YAEMSAAVSRYANALGVLGVEPGDRVTVQVEKSVSGVLLYLAVMKAGAIYQPLNTAYT
                     EAEVDYFVGDAEPKLIVCDPARQQRLRAIADAHKVFAVVNLDGRGEGSLAALADQMDE
                     RHDTVERSAEDLAGLLYTSGTTGRSKGAMITHGNLAANATTLVKLWQMSDSDVLLHAL
                     PVFHVHGLYVALNTCFLAGTEIIWFDRFDAAAMIDAMPRATLMMGVPTFYTRLLATPA
                     LSREATRNMRLFISGSAPLLTETHHAFSARTGHAILERYGMTETGMITSNPYDGARVP
                     GSVGFALPDVSVRIRGDQPGVIEVKGPNVFKGYWRMREKTRDEFTADGYFITG"
     gene            complement(1302..2468)
                     /locus_tag="AC16A0_04805"
     CDS             complement(1302..2468)
                     /locus_tag="AC16A0_04805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3104684.1"
                     /translation="MLKINPAVAAIEEETAFTVLDRANALRAKGLPVINLGIGQPDFK
                     PPKHILEAAEKAVRDGPHGYSSPQGLPILRDAVAQYVGNRFKVPVTGDEVVIVPGGKV
                     TFYFACQIFGGPGAEILYPDPGFPPYRDAARSSGATPVAYPIREEKDFGFDAEEVLSL
                     ITPATRLIIINSPANPTGGVVSRQEITRLAEGLLKHPQVAILSDEIYSHQVYDGVDFT
                     SFLGFPELRDRTIILDGWSKTFAMTGWRLGFGIWPRHLVDYVKKLITVDHSCTSVATQ
                     MAGLAAIAGPMDEIEGFRRSFQTRRDLVVAGLNDIKGVRCRVPGGAFYAFPNITGTGW
                     SSRDLSRELLEKAHVALLLGESFGANGAGFVRISYAASETELREALARMKAFLG"
     gene            complement(2468..3331)
                     /locus_tag="AC16A0_04810"
     CDS             complement(2468..3331)
                     /locus_tag="AC16A0_04810"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104685.1"
                     /translation="MQVGIVGAGLMGHGMAANLLKHGHGVTVIAHRNRAPVDDLVSKG
                     AREARDMAELAEADAILLCVTTSKVVADTIARLKPHLRRGQIILDSGTSAPEETRRLA
                     HDLEAMGVGFADIPLTGGPEQAEQGELGVLCGASDKTFAAIGPMLGCFATTVRHFGPP
                     GSGHTAKLISNYLVTGMVALVAEAFGAAHRAQIDWKDLYEVMLNGSGNSGVLRKMVGP
                     ALEGNFDGYRFALANAAKDIGYYEEMAGKLGCATRLTESVAEIFRQAVETGHGGRNVS
                     HLLDPAIDDVT"
     gene            3389..4828
                     /locus_tag="AC16A0_04815"
     CDS             3389..4828
                     /locus_tag="AC16A0_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3104686.1"
                     /translation="MMALHKNYINGEWVEGKGVRENINPSDLTDVVGEYAQADAGQAE
                     AAIAAAKAAQPGWAASTPQAKADALEAIGVELLARKDEIGRLLSREEGKPLANGIAET
                     MRAAQIFKFFAQEALRVEGVAITSVRPGVDVMLTREPLGVVGLICPWNFPIAIPAWKM
                     APALAFGNTVVLKPADLVPGTAWALMEIISRAGLPKGVVNLVMGRGSVVGQTMLDSKD
                     VNAISFTGSVETGARVAMACARRGAKFQLEMGGKNPLVVLDDANLDAAVAAAIDGAFY
                     QTGQRCTASSRLIVQKGIHDAFVDKMVATMKTLKVDHALKDGTVIGPVVDEGQMAQDE
                     KYIQIARDEGGELAWGGERLNRDTKGHYLSPALFVGTKNSMRINREEVFGPVASVIKV
                     DSYEEGLAVANDTAFGLSSGIFTTSLKHATDFRKKSQAGMAMINLPTAGVDYHVPFGG
                     RKGSSYGAREQGRHAVDFYTAIKTAYTLA"
     gene            complement(4829..5563)
                     /locus_tag="AC16A0_04820"
     CDS             complement(4829..5563)
                     /locus_tag="AC16A0_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HIR3104687.1"
                     /translation="MSGPLPYLPLDMGWPNLLAIALIFGLWGLYTPILKLIGKGSLNS
                     QLHDVRLRWLQMHQGIHREHRVFDAILLGHISNSISYFGSATLLVLAGLVGSLVNVSR
                     IYQVTRDLSFFDQGMTAESFTLCFALLTLIMALCFFAFTYALRKMAYTFAMLGGLQAS
                     PAHNEETRIMGEQAAIVLTEAVRSINNGIRGFYYAIAALFLFSGPYVCIAATIVITAI
                     LYYRQMLSPTARAIATYVAALKRLGS"
     gene            complement(5560..>6928)
                     /locus_tag="AC16A0_04825"
     CDS             complement(5560..>6928)
                     /locus_tag="AC16A0_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR3104688.1"
                     /translation="VMTFEYADRVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAK
                     GIEARTRKLFEICRLRDIPIITFVNKMDREARDPLDLLDEIEKGLALTVAPMVWPIGQ
                     GKDFAGTYDLQTPRVRQLEKAPEGVPVSGPDDPLIATLITGDTLTKWRDEIELVRDLC
                     HSFDRQAFLEGHLSPVYFGSALKNFGVRDLLEALIAYAPPPRDQSASARMVHATEPRM
                     TGIVFKIQANMDPNHRDRIAFMRLASGKLTRGMKVKLVRTGKTIALTAPQFFFAQDRS
                     IADEAYAGDVVGIPNHGLLRIGDTLTEGEDIVFTGVPSFAPEILRRVKLGDPMKAKKL
                     REALEQLAEEGVVQLFIPADGSPAIVGVVGALQLDVLADRLNSEYGVPVSFEPARYEV
                     MRWISCEDAKKLDEFVTANRFSIAHDLDGDPVFMAQSAFSLNYTLERAPEIKVTEIKE
                     MHKAA"
BASE COUNT         1211 a         2239 c         2278 g         1200 t
ORIGIN      
        1 cgccagtgat gaagtacccg tccgcagtga actcatctct ggtcttttcc cgcatgcgcc
       61 agtagccctt gaagacgttg ggccccttca cctcgatcac gccgggctgg tccccgcgga
      121 tgcggacgga cacatccggg agcgcgaagc cgacgctgcc gggcacgcgc gcgccgtcat
      181 agggattgga ggtgatcatg ccggtttcgg tcatgccgta ccgctcgagg atggcgtggc
      241 ccgtgcgggc gctgaaggcg tgatgcgtct cggtgaggag cggcgcggaa cccgagatga
      301 aaagccgcat gttgcgcgtg gcctcgcggg agagggcggg cgtggcgagc agccgcgtgt
      361 agaaggttgg cacccccatc atgagggttg cccgcggcat ggcgtcgatc atcgccgcgg
      421 catcgaagcg gtcgaaccag atgatctccg ttccggcgag aaagcaggtg ttgagggcca
      481 catagagtcc gtggacgtga aataccggca gcgcatgcag cagcacgtca gagtcgctca
      541 tctgccagag cttcaccagg gtcgtggcat tcgccgcgag gttgccatgg gtgatcatgg
      601 cacccttcga ccgtccggtg gtgccggacg tgtagagcag ccctgccaga tcctcggccg
      661 atcgttccac cgtatcgtgg cgctcgtcca tctggtctgc gagcgcggcc agcgatccct
      721 cgcccctgcc gtcgagattg accaccgcga agaccttgtg tgcgtcggcg atggcgcgga
      781 gcctctgctg ccgtgccgga tcgcacacga tcagcttggg ttcggcatcg ccgacgaagt
      841 agtccacctc ggcttcggta taggcggtgt tgagcggctg gtagatggct ccggccttca
      901 tcacggcgag gtagagcaag acgccggaga ccgacttctc gacctgcacc gtcacgcggt
      961 cgccgggctc gacgccgagg acgccaagcg cattggcgta gcggctcacc gccgccgaca
     1021 tctcggcata ggtcatcgtg ctctggccgt cggccgggcg gatggcgaca cgcgcaccct
     1081 gctgggcgaa gccctcttcg aagaggtggt agagattgcc gtttgccacg atgaattgtg
     1141 ccccgccttc cgctttcgtc atctgtttgt catcgggcgc gtgccaagaa ggtggcgcgg
     1201 gtgtcccaac ttgcccgcca gccccaactg aaagccatgc cggggcgctg tcggttcctt
     1261 agcccccctt cccaccgcag cgccggcatg gcgccccttt ttcagccaag gaacgccttc
     1321 atgcgcgcca gggcctcgcg caattcggtt tcgctagcgg cataggatat ccgcacgaag
     1381 cccgcaccat tggcgccgaa gctctcgccc agaagcaagg ccacatgcgc cttctcaagc
     1441 agttcgcgcg acaggtcgcg cgacgaccag ccggtgccgg tgatgttcgg aaaggcgtag
     1501 aaggcgccgc ccggcacgcg gcagcgcacg cccttgatgt cgttcagccc ggccaccacc
     1561 aggtcgcgcc gcgtctggaa gctcctgcgg aagccttcga tctcgtccat cgggccggca
     1621 attgcggcaa gccctgccat ctgggttgcc acactggtgc aggaatggtc gacggtgatc
     1681 agcttcttca catagtccac caggtgccgt ggccagatgc cgaagcccag gcgccatcct
     1741 gtcatcgcga aggtcttgga ccatccgtcg agaatgatgg tgcggtcgcg cagctccgga
     1801 aagccgagga agctggtgaa gtccaccccg tcatagacct ggtggctgta gatctcgtcc
     1861 gagaggatgg cgacctgcgg atgcttgagc agtccttcgg ccagccgcgt gatctcctgg
     1921 cgcgagacca cgccgccggt gggattggcg ggcgagttga tgatgatgag gcgggtggcc
     1981 ggggtgatca gcgagagcac ttcctcggca tcgaatccga agtccttttc ctcgcggatg
     2041 ggataggcca cgggcgtggc cccgctcgac cgggctgcgt cgcggtaggg cgggaagccc
     2101 ggatcgggat agaggatctc ggcgcccggc ccgccgaaga tctggcaggc gaaatagaaa
     2161 gtcaccttgc cgccgggcac gatcacaacc tcgtcgccgg tcaccggcac cttgaagcgg
     2221 ttgccgacgt attgcgccac cgcatcgcgc aggatgggca ggccctgcgg cgagctatag
     2281 ccatgcgggc cgtcgcgcac cgccttctcg gccgcttcga gaatgtgttt cggcggcttg
     2341 aagtcgggct ggcctatgcc gaggttgatg accggcaggc ccttggccct gagcgcattg
     2401 gcgcggtcca gcaccgtgaa ggcggtctct tcttcgatgg ccgcaacggc cggattgatt
     2461 ttcagcatca ggtcacatca tctatggctg gatcgagcag gtggctgaca ttgcgtccgc
     2521 cgtgacccgt ttctacggcc tgccggaaga tttccgcaac ggattccgtc aggcgcgtcg
     2581 cgcaacccag cttcccggcc atctcctcgt agtagccgat gtccttggcg gcattggcaa
     2641 gggcgaagcg gtagccgtcg aagttcccct ccagcgccgg acccaccatc ttgcgcaaca
     2701 cgccggaatt gccggagcca ttgagcatga cctcgtaaag atccttccag tcgatctgcg
     2761 ccctgtgcgc ggcgccgaag gcttccgcaa ccagcgccac catgccggtg accaggtagt
     2821 tggagatcag cttggccgta tgccccgagc ccggcgggcc gaagtgccgc acggtcgtgg
     2881 caaagcagcc gagcatgggt ccgattgcgg cgaaggtctt gtcgctcgcc ccgcacaaga
     2941 cgccaagctc gccctgctcc gcctgctccg gaccgccggt cagcgggatg tcggcgaagc
     3001 ccacgcccat cgcctcgagg tcatgtgcca ggcgccgggt ttcctccggc gcggaggtgc
     3061 cggaatcgag aatgatctgc ccccgccgca gatggggttt cagccttgcg atggtgtccg
     3121 caaccacctt cgacgtcgtg acgcacagaa ggatcgcatc ggcctctgcc agctccgcca
     3181 tgtcgcgtgc ttcgcgcgcc cccttcgaca ccagatcgtc caccggcgcc cggttacgat
     3241 gggcaatgac ggtcacgcca tggccatgct tcaagagatt ggctgccatg ccatgtccca
     3301 tcaatcccgc cccgacgatg cccacctgca tgcttgcctc tcccgcgctc cctgcctatg
     3361 gtccggcccg aaaatcaggg agttccgcat catggcgctg cacaagaatt acatcaatgg
     3421 cgaatgggtc gagggcaagg gcgttcgcga gaacatcaac ccgtcggact tgaccgatgt
     3481 ggtgggcgaa tatgcccagg ccgatgcggg ccaggccgag gccgccattg ccgccgccaa
     3541 ggccgcacag cccggctggg ccgccagcac gccgcaggcc aaggccgacg cgctggaagc
     3601 catcggcgtc gaactcctcg cccgcaagga cgagatcggc aggttgctct cgcgcgagga
     3661 gggcaagcca ctcgccaatg gcattgccga gaccatgcgt gccgcccaga tcttcaagtt
     3721 ctttgcgcag gaagcgctac gcgtcgaggg cgtcgcaatc acgtcggtgc gccccggcgt
     3781 cgacgtgatg ctgacccgcg agccgctggg cgtcgtcggt ctgatctgcc cgtggaactt
     3841 ccccatcgcg atccccgcct ggaagatggc gccggcgctg gccttcggca acacggtggt
     3901 gctgaagccc gccgacctgg tgcccggcac ggcctgggcg ctcatggaga tcatctcgcg
     3961 cgccggactg ccgaagggcg tggtgaacct cgtcatgggc cgcggctccg tcgtgggcca
     4021 gaccatgctg gactcgaagg acgtcaacgc catctccttc acgggttcgg tcgagaccgg
     4081 tgcccgggtc gccatggcct gcgccaggcg cggcgccaag ttccagctcg agatgggcgg
     4141 caagaacccg ctggtggttc tggatgacgc caatctcgat gcggccgtgg ccgccgccat
     4201 cgacggcgcc ttctaccaga ccggccagcg ctgcaccgcc tcgtcgcgcc tcatcgtgca
     4261 gaagggcatc cacgatgcct tcgtcgacaa gatggtcgcc acgatgaaga cgctcaaggt
     4321 cgaccacgcg ctgaaggacg gcaccgtcat cggcccggtg gtcgacgaag gccagatggc
     4381 gcaggacgag aagtacatcc agatcgcccg cgacgagggc ggcgagctgg cctggggcgg
     4441 cgagcgcctc aaccgcgaca ccaagggcca ttatctgagc cccgcgcttt tcgtgggaac
     4501 gaagaacagc atgcggatca atcgcgagga agtctttggc cccgtcgcct cggtgatcaa
     4561 ggtcgactcc tacgaggaag gcctggcggt cgccaacgac acggccttcg gcctctcgtc
     4621 gggcatcttc acgacctcgc tcaagcatgc caccgacttc cgcaagaagt cgcaggccgg
     4681 catggcgatg atcaacctgc cgaccgcggg cgtcgattac cacgtgccct tcggcggccg
     4741 gaagggttcg agctacggcg cgcgcgagca gggacgtcac gcggtggatt tctacacggc
     4801 catcaagacg gcctatacgc tggcctgatc aactgcccag ccgcttcagc gccgcgacat
     4861 aggtggcgat cgcccgcgcc gtgggcgaca gcatctgcct gtagtacagg atcgccgtga
     4921 tgacgatcgt cgcggcgatg cagacgtagg ggcctgaaaa caggaacagc gcggcaatcg
     4981 catagtagaa gccgcggata ccgttgttga tgctgcgcac cgcttcggtg agcacgatgg
     5041 cggcctgttc gcccatgatc cgcgtttcct cgttgtgcgc gggcgaggct tgaagtccgc
     5101 ccagcattgc gaaggtatag gccatcttgc gcagcgcata ggtgaaggcg aagaagcaca
     5161 gcgccatgat cagcgtgagc agcgcaaaac agagtgtgaa ggactctgcc gtcatgccct
     5221 gatcgaagaa ggagaggtcg cgcgtcacct gatagatgcg cgagacattg accagcgagc
     5281 cgacgagtcc ggccagcacc agcagcgtcg ccgaaccgaa gtacgagatg gaattggaga
     5341 tgtggcccag caggatcgcg tcgaagacgc ggtgctcgcg atgaatgccc tgatgcatct
     5401 gcagccagcg cagccgcacg tcgtgcagct ggctgttgag cgaacccttg ccgatgagtt
     5461 tcaggatcgg cgtgtagagt ccccagagtc cgaagatcag ggcaatggcc agaagattgg
     5521 gccatcccat gtcgagcggc agatagggaa ggggcccgct catgccgcct tgtgcatttc
     5581 cttgatctcc gtcaccttga tctccggcgc gcgttccagc gtgtaattca ggctgaaggc
     5641 cgattgcgcc atgaagaccg ggtcgccgtc aagatcatgg gcgatggaga accggtttgc
     5701 ggtgacgaac tcgtcgagct tcttcgcgtc ctcgcaggag atccagcgca tcacctcgta
     5761 gcgcgccggc tcgaagctta ccggcacgcc atattcgctg ttgagccggt cggccagaac
     5821 gtcgagctgc agcgcaccca ccacgccgac gatggcggga gagccgtccg ccggaatgaa
     5881 gagctgcacg acgccctcct ccgccagctg ctccaacgcc tcgcgcagtt tcttggcctt
     5941 catcgggtcg cccagcttca cgcggcgcag gatttccggc gcgaagctcg gcacgccggt
     6001 gaagacgatg tcctcgccct ccgtcagcgt gtcgccgatg cgcagcaggc catggttcgg
     6061 aatgcccacc acgtcgccgg cataggcctc gtcggcgatg gaacggtcct gggcaaagaa
     6121 gaactgcggc gcggtcagtg cgatggtctt accggtgcgc accagcttga ccttcatgcc
     6181 gcgcgtcagc ttgcccgagg ccagccgcat gaaggcgatg cggtcgcgat ggttcgggtc
     6241 catgttggcc tggatcttga acacgatgcc cgtcatcctg ggctcggtcg catgcaccat
     6301 gcgggccgag gccgattggt cgcgcggcgg cggcgcgtaa gcgatcaatg cctccagcag
     6361 gtcgcgcacg ccgaaattct tgagcgccga gccgaagtag acgggcgaaa gatggccttc
     6421 gaggaaggcc tggcgatcga agctgtggca gaggtcgcgc accagctcga tctcgtcgcg
     6481 ccacttggtc agcgtgtcgc ccgtgatcag cgtggcgatc agcggatcgt ccggtccgga
     6541 gactggaacg ccttccggcg ccttctcgag ctggcgcacg cgcggcgtct ggagatcgta
     6601 ggttccggcg aagtccttgc cctggccgat gggccagacc atgggcgcca cggtcagcgc
     6661 caggcccttc tcgatctcgt cgagcaggtc cagcggatcg cgggcctcgc ggtccatctt
     6721 gttgacgaag gtgatgatcg gaatgtcgcg cagccggcag atctcgaaga gcttgcgggt
     6781 gcgcgcctcg atgcccttgg ccgcgtcgat caccatgacg gcgctgtcga cggcggtgag
     6841 cgtgcgatag gtgtcttccg agaaatcctc gtggcccgga gtgtcgagca ggttgaacac
     6901 gcggtcggca tactcgaacg tcatcacg
//
LOCUS       DBNGVR010000219         2603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2633766, whole genome shotgun sequence.
ACCESSION   DBNGVR010000219 DBNGVR010000000
VERSION     DBNGVR010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2633766"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2540)
                     /gene="acnA"
                     /locus_tag="AC16A0_04830"
     CDS             complement(<1..2540)
                     /gene="acnA"
                     /locus_tag="AC16A0_04830"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3104689.1"
                     /translation="MSLDSFKSKKTLKVGAKTYTYFSLKAAEKNGLKGISKLPYSLKV
                     LLENLLRFEDGRSVTKDDIAGIARWLKNRGRDEKEIAFRPARVLMQDFTGVPAVVDLA
                     AMRDAMKGLGKDPKKINPLAPVDLVIDHSVMVDFFASSKAFKANVNLEYDRNGERYTF
                     LKWGQGAFDNFRVVPPGTGICHQVNLEYLARTVWTREEKVKGKKVTYAYPDTLVGTDS
                     HTTMVNGLAVLGWGVGGIEAEAAMLGQPISMLIPEVIGFKLTGALPEGTTATDLVLTV
                     TQMLRKKGVVGKFVEFYGPGLAHLSLEDMATIANMAPEYGATCGFFPINAETIAYLKG
                     TARASQQVALVEKYAKAQDMYWTPKAADPEFTDTLTLDLGSVVPSIAGPKRPQDRVVL
                     AEAATGFAKVMADEFKKAEELGRRVKVESANFDLGHGDVVIAAITSCTNTSNPSVMIG
                     AGLLARNAVKKGLASKPWVKTSLAPGSQVVTEYLASSGLQKDLDKIGFNLVGYGCTTC
                     IGNSGPLPENISEAIRKNDLVAAAVLSGNRNFEGRVNPDVRANYLASPPLVVAYALAG
                     SLNVNLATDPLGHDKTGKPIYLRDIWPTAKEIGDFVRKNVTRKAFAKRYKDVFKGDAQ
                     WRKIKVTKGMNYAWDAASTYVANPPYFDGLTMTPAPVKDVESARILGLFLDSITTDHI
                     SPAGDIKASSPAGAYLQSRGVEKLDFNSYGARRGHHEVMMRGTFANIRIKNQMVPGVE
                     GGVTRHYPDGTQMPIYDAAMKYEAEGVPLVIFAGREYGTGSSRDWAAKGTRLLGVRAV
                     IAQSFERIHRSNLIGMGVVPLQFREGESWQTLGLKGDETVTIKGLAEGL"
BASE COUNT          428 a          809 c          861 g          505 t
ORIGIN      
        1 aggccctcgg ccaggccctt gatcgtgacg gtctcgtcgc ccttcaggcc cagcgtctgc
       61 cagctctcgc cctcgcggaa ctgcagcggc accacgccca tgccgatgag gttcgaacgg
      121 tggatgcgct cgaagctctg ggcgatgacg gcgcggacgc cgagcaggcg cgtgcccttg
      181 gccgcccagt cgcgcgagga gccggtgccg tattccctgc cggcgaagat caccagcggc
      241 acgccttcgg cctcgtactt catggctgca tcgtagatcg gcatctgcgt accgtccgga
      301 tagtggcggg tcacgccgcc ttccacgccg ggcaccatct ggttcttgat gcggatgttg
      361 gcgaaggtgc cgcgcatcat cacctcgtga tggccgcgcc gcgcgccgta ggagttgaag
      421 tcgagcttct cgacgccgcg cgactgcaga taggcgccgg ccggcgacga ggccttgatg
      481 tcaccggcgg gcgaaatgtg gtcggtggtg atcgagtcga ggaagagtcc gaggatgcgc
      541 gcgctctcca catccttcac cggcgccggc gtcatggtga ggccgtcgaa gtagggcggg
      601 ttcgccacat aggtggaggc cgcatcccag gcatagttca tgcccttcgt caccttgatc
      661 ttgcgccact gcgcatcgcc cttgaagacg tccttgtagc gcttggcgaa ggccttgcgc
      721 gtcacgttct tgcgcacgaa gtcgccgatc tccttggccg ttggccagat gtccctcaga
      781 tagatgggct tgcccgtctt gtcgtggccg agcgggtcgg tggcgagatt gacgttcagc
      841 gatccggcca gtgcataggc gacaaccagc ggcggggacg ccagatagtt ggcgcgcaca
      901 tcggggttca cgcggccttc gaagttgcgg ttacccgaca gcacggccgc ggccaccaga
      961 tcgttcttgc ggatcgcctc agagatgttc tcgggtagcg ggccggagtt gccgatgcag
     1021 gtggtgcagc catagcccac gaggttgaag ccgatcttgt cgagatcctt ctggaggccc
     1081 gaggaggcga gatactcggt caccacctgc gagccgggcg ccagcgaggt cttgacccag
     1141 ggcttcgagg cgaggccctt cttcaccgca ttgcgcgcca ggaggccggc gccgatcatc
     1201 acgctgggat tggaggtatt ggtgcaggac gtgatggcgg cgatcaccac gtcgccatgg
     1261 cccaggtcga aattggcact ctccaccttc acccggcggc ccagttcttc ggccttcttg
     1321 aactcgtcgg ccatgacctt ggcgaaaccg gtggcggcct cggccagcac aacacggtcc
     1381 tgcggccgct tcggcccggc aatggaggga acgacgctgc cgagatcgag cgtcagcgtg
     1441 tcggtgaatt ccggatccgc tgccttgggc gtccagtaca tgtcctgggc cttggcatat
     1501 ttttcgacca gcgccacctg ctgcgaggcg cgcgccgtgc ccttgagata ggcgatggtc
     1561 tcggcattga tcgggaagaa accgcaggtg gcgccatatt ccggcgccat gtttgcgatg
     1621 gtggccatgt cctcgagcga gagatgcgcg aggcccggcc cgtagaactc cacgaacttg
     1681 ccgaccacgc ccttcttgcg cagcatctgg gtgacggtga gcacgagatc ggtggccgtg
     1741 gtaccctcgg gcagcgcgcc cgtcagcttg aagccgatga cttcagggat gagcatggag
     1801 atgggctggc ccagcatggc ggcttccgcc tcgatgccgc cgacgcccca gcccagcacg
     1861 gccaggccgt tgaccatggt ggtgtgcgag tcggtgccga ccagcgtgtc gggataggca
     1921 taggtgacct tcttgccctt caccttctcc tcgcgggtcc acacggtgcg ggcgagatat
     1981 tcgagattca cctgatggca gatgccggtg ccgggcggca cgacgcggaa gttgtcgaag
     2041 gcgccctggc cccacttgag aaaagtgtag cgctcgccgt tgcggtcgta ttcgagattg
     2101 acgttggcct tgaaggcctt cgaactggcg aagaagtcca ccatcaccga gtggtcgatg
     2161 acgagatcga ccggcgccag cgggttgatc ttcttcggat ccttgcccag acccttcatc
     2221 gcgtcgcgca tcgcggcgag atcgaccacg gcgggcacgc cggtgaagtc ctgcatcagc
     2281 acgcgggccg ggcggaaggc gatctccttc tcgtccctgc cgcggttctt gagccagcgg
     2341 gcgatacccg cgatatcatc cttggtgacc gagcggccgt cctcgaagcg cagcaggttt
     2401 tccagcaata ccttcagcga atagggcagc ttcgaaatgc ccttgaggcc gttcttctcg
     2461 gcggccttca ggctgaaata ggtataggtc ttcgcaccga ccttgagggt cttcttggac
     2521 ttgaagctgt cgagggacat cggggcctca tgatgctggg gatttggcgt gagcctatag
     2581 tatcttttgc actgcaacac cag
//
LOCUS       DBNGVR010000220         6847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_80754, whole genome shotgun sequence.
ACCESSION   DBNGVR010000220 DBNGVR010000000
VERSION     DBNGVR010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_80754"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="AC16A0_04835"
     CDS             complement(<1..456)
                     /locus_tag="AC16A0_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3104690.1"
                     /translation="MGFSLSVNTNPLVNRFAEPEDLIGTLAEEVGIGHIQLVHEFVNP
                     SWPAATVKRLTDRLARACAARRAHITSIMTGPYGRLNHFAHPDPDVRAYYLRWFKGMA
                     DIAGDLGAPAIGTQFGIFTFRDYDDAHRRATLMQVALDCWRELAAHARAR"
     gene            594..1577
                     /locus_tag="AC16A0_04840"
     CDS             594..1577
                     /locus_tag="AC16A0_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIR3104691.1"
                     /translation="MDEEAHLAARAAWLHFAGGKTQGEVAELLGVQSTKAHRLIARAR
                     SEGLIRVFVEGPIAGCIDLEEKLKGAFGLQLCEVVPNVDEGALPLRTLAIAGARYIRN
                     LIESGRYSLIGMGHGRTLAAMVDAMPSVAAEGTRFVSLLGGLTRRFAASPFDVIHKLA
                     ERTGAESYVMPVPFFANTAKDREVLESQFGVSNVIDMARRAEIYIAGIGEVDRKSFIA
                     TAGMVDDEDVDEVVQSGACAEILGQFFTAEGRHIPNGVSQRALAPRLSDLQTHKIVAV
                     AGGTSKTQAIRAILGSGLLHGLITDEATARRLVNAKPGREPGKKNGKSAPG"
     gene            1613..3052
                     /locus_tag="AC16A0_04845"
     CDS             1613..3052
                     /locus_tag="AC16A0_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104692.1"
                     /translation="MYDKEKGLFEAFVAKKMSRRELIQTTGKLGLSASASGFLLTQAQ
                     TQAMAADFDWQKYKGSKLKLLLNKHPYADAMIANIETFKTMTGMDISWDIFPEDVYFD
                     KVTAALSSGSTEYDAFMTGAYMTWTYGPAGWITDLNEYIKDPAKTNPNYAWDDVLPGL
                     RASTSWNGVPGGELGSADAKQWCIPWGYELNNLSYNKTMFDKVGVQPPKNMGELLEVS
                     AKLTKDLGGPYGVGVRGSRSWATIHPGFLSGYANFGEKDFNVADGKLKAAMNSAGSKA
                     SHADWVKMIQDSGPKNWSTYTWYQVGTDLGAGASAMIFDADILGYFMNGGDNKEKGNL
                     GYAPFAANPNAAAPTPNVWIWSLAMNNAGTQKDATWYFLQWATSVEHDLFGARKMDFV
                     NPVRASVWKDEEFRNRIDKSYPGYLNQHVVSAPGAKIYFTAQPLFFDLTTLWAENLQK
                     MVAKEIPVDEGLDKMAAEIDAKLAEAGLG"
     gene            3152..4078
                     /locus_tag="AC16A0_04850"
     CDS             3152..4078
                     /locus_tag="AC16A0_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3104693.1"
                     /translation="MTAAANQIHAPVKQSRFMRRALPYILSLPALLMCIGILIPFFTA
                     VYYSLLRYRMNLPQMRGFIWFDNYINFFADPKFWNTVYISLVYAALTVGLELIIGLGI
                     ALLLQRPTRFNNIASILLLLPLMTAPALAALMWKLMTHPDFGILSYLLTLIGITDFKW
                     ASDPSTALLTVVLVDIWVYTPFIMILLLAGLRSLPKAPFEAAALDGVPRSFVFFRITL
                     PMLMPYIITATLFRLLDSIQQFDIIYAMTQGGPGDKLLVFQVQAYLDFFQATNVGRSA
                     ALMIILWAITYALSNVFIKQWLKLRERAHGAG"
     gene            4082..4939
                     /locus_tag="AC16A0_04855"
     CDS             4082..4939
                     /locus_tag="AC16A0_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3104694.1"
                     /translation="MDHTSFLERLIRGILITLVLVFFLFPIVWILLMSLQTNETILRI
                     PPSLVFTPIFDNYTALVTGQLRTAAGSLQLNFMQNLWNSVLLSSASVLLSLILGVPAA
                     YAFARFKFRLGEDIAFTLLSFRFAPPLLVLLPLSLYFKDLGLTNTYIGLIWVYQLICL
                     PLILWIVRGYFEDISPDIEHAYRIAGNSWWKTFRKIAVPLARPGIAAAGLLAFIFAWN
                     NFVFALILASADKQPVTVGALAFVTASGIQYGQIAAAIVLSVTPTLALALYAQRYLVE
                     GLSMGAVKG"
     gene            4936..6018
                     /locus_tag="AC16A0_04860"
     CDS             4936..6018
                     /locus_tag="AC16A0_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104695.1"
                     /translation="MSQLSLKALSKSYGAFKVFEGLDLSAEAGEIVVVFGGSGTGKTI
                     LLRLIAGVEEPTAGSILIGGHDVSDIAPEHRGVGMAFQNFALFPHMSAAENIATPLRA
                     KGESEQAVQSGIAKVAKLLKIDHVLGHAPRELSNGQKQRTALARALAAEPRILLLDDP
                     LRNVDAKLRFEMRLELPRLLRQSGATVLYVTQDYKEAMALGDRIAVLAGGKFVQVATP
                     EDIYVKPASLSVARLFGDPSINTAETVVKAGGKGAGLSLGGTEVAIAAEYKGLAGKRV
                     VFGIRPEAIEIKSSKSSGIPAEIVAVTPLNERTVLLLKAADGWEFLASLPASAKAPEP
                     GTKVAASFASSDIHFFDTASGERLHG"
     gene            6011..>6847
                     /locus_tag="AC16A0_04865"
     CDS             6011..>6847
                     /locus_tag="AC16A0_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104696.1"
                     /translation="MGELVISKVDKIYRGRKTAVHAVKALDLTVKPGEIVALLGSSGC
                     GKTSTLRMIAGFEDVTSGSITLDGKPIHQLPPAQRGVAMAFEAYSLYPPLTIGENIAF
                     ALKASRLSAAEQESKVRAMAGMLEIDGILGKYPSSVSGGQQQRASLGRALVRKAGLYL
                     LDEPMGQLEPQLRAILRGRIKHYLRENACTTILVTHDQTEANALADRIAVMEGGELQQ
                     YATPAELKARPANLFTGTFIGEPPMNVFPATLSINSRTVSFDIAGGAALSYPAAELSR
                     PVR"
BASE COUNT         1249 a         2315 c         2067 g         1216 t
ORIGIN      
        1 tcgggccctc gcatgcgcgg caagctcgcg ccagcagtcg agagcgacct gcatcagcgt
       61 tgcgcggcgg tgcgcatcgt catagtcgcg aaaggtgaag atgccgaact gggtgccgat
      121 ggccggcgcg ccgaggtcgc cggcaatatc cgccatgccc ttgaaccagc gcagatagta
      181 ggcgcgcaca tcgggatcgg gatgggcgaa gtgattgagc cggccatagg gcccggtcat
      241 gatcgaggtg atgtgcgcgc gccgggcggc acaggcccgt gcgaggcgat cagtcaggcg
      301 tttcaccgtt gccgcgggcc aggacggatt gacgaattca tgtaccagct ggatgtggcc
      361 gatgcccacc tcctcggcca gcgtgccgat cagatcttcc ggctccgcaa agcggttgac
      421 cagcggattg gtgttcaccg agagcgagaa tcccattggc cgtccctgtc cctgctgcgt
      481 tgcagcgacg atttttgttg acgccgaggc tagtatgaaa aatattgcat ctcaagaaaa
      541 ataattcaaa accgaatggg aagcgattga acctgcacgc cacatcactt cacgtggacg
      601 aggaggcaca tcttgccgcc cgcgccgcgt ggttgcattt cgccggcggc aagacccagg
      661 gcgaggtggc ggaactgctc ggcgtgcaga gcaccaaggc ccatcggctg atcgcacggg
      721 cacgcagcga aggcctcatc cgggtcttcg tcgaaggccc gatcgccgga tgcatcgacc
      781 tcgaagagaa actgaagggt gccttcgggc tgcagctctg cgaggtggtg ccgaatgtcg
      841 acgaaggcgc cttgcccctg cgcacgctgg ccatcgccgg cgcgcgctac atacgcaacc
      901 tcatcgagag cggccgctat agcctgatcg gcatgggcca tggacgcacg cttgccgcca
      961 tggtggacgc catgccgagc gtggcggccg aaggcacgcg cttcgtttcg ctgctgggcg
     1021 gactgacccg gcgcttcgca gcaagcccct tcgacgtgat ccacaagctg gccgagcgca
     1081 cgggtgcgga gtcctacgtg atgcccgtgc ccttcttcgc caacaccgcc aaggaccgag
     1141 aagtcctgga gtcccagttt ggcgtctcga acgtcatcga catggcgcgg cgcgccgaga
     1201 tctatatcgc cggcatcggc gaggtcgacc gcaagagctt catcgccacc gccggcatgg
     1261 tcgacgacga ggatgtcgac gaggtggttc agtcgggggc ctgcgccgag atcctcggcc
     1321 aattcttcac cgccgagggc cggcacattc ccaatggcgt gtcacagcgc gcactcgcac
     1381 cgcggctttc cgatctgcag acgcacaaga ttgtcgccgt cgccggcggc acatccaaga
     1441 ctcaggccat ccgcgccatt ctcggaagcg gccttcttca tggcctgatc accgacgagg
     1501 caaccgcacg ccgcctcgtc aacgccaaac cgggacgtga gcccggcaag aagaacggaa
     1561 aatccgcacc gggctaactt tcgtgaacca acgaaacaac ggaggaatgg gaatgtacga
     1621 taaagaaaag ggcctcttcg aagccttcgt ggcgaagaag atgagccgcc gcgaattgat
     1681 ccagacgacc ggaaagctgg gcctctcggc cagcgcctcc ggcttcctgc tgacccaggc
     1741 gcagacacag gccatggcag cggacttcga ctggcagaag tacaagggct cgaagctgaa
     1801 gctcctgctc aacaagcatc cctatgcgga tgcgatgatc gccaacatcg agaccttcaa
     1861 gaccatgact ggcatggaca tctcctggga catcttcccg gaggatgtct acttcgacaa
     1921 ggtcaccgcg gcgctctcct cgggctcgac cgagtatgac gccttcatga ccggcgccta
     1981 catgacctgg acctacggcc cggccggctg gatcaccgac ctcaacgagt acatcaagga
     2041 cccggccaag accaacccga actatgcctg ggacgacgtg ctccccggcc tgcgggcgtc
     2101 gacctcctgg aacggcgttc ccggcggcga actgggttcg gcggatgcca agcagtggtg
     2161 cattccctgg ggctacgagc tcaacaacct gtcctacaac aagaccatgt tcgacaaggt
     2221 cggtgtccag ccgcccaaga acatgggcga actcctggaa gtctcggcca agctcaccaa
     2281 ggatctgggc ggaccctatg gcgtcggcgt gcgcggctcg cggagctggg ccacgatcca
     2341 tccgggcttc ctctcgggct atgccaactt cggcgagaag gacttcaacg tcgccgacgg
     2401 caagctgaag gccgccatga actcggcggg ctcgaaggcc tctcatgctg actgggtgaa
     2461 gatgatccag gactcgggcc cgaagaactg gtcgacctac acgtggtacc aggtcggcac
     2521 cgatctcggc gccggcgcct ccgccatgat cttcgacgcc gacatcctcg gctacttcat
     2581 gaacggcggc gacaacaagg agaagggcaa cctcggctac gcgcctttcg cggcgaaccc
     2641 gaatgccgcg gcacccacgc cgaacgtctg gatctggtcg ctggccatga acaatgccgg
     2701 cacccagaag gacgccacct ggtacttcct gcagtgggcc acctccgtcg agcacgacct
     2761 gttcggcgcc cgcaagatgg acttcgtcaa tccggtccgc gcctccgtct ggaaggacga
     2821 ggaattccgc aaccgcatcg acaagtccta tccgggctac ctcaaccagc atgtcgtgtc
     2881 ggcaccgggc gcgaagatct acttcaccgc ccagccgctg ttcttcgacc tcaccaccct
     2941 gtgggccgag aacctgcaga agatggtggc aaaggaaatc ccggtcgacg aaggcctgga
     3001 caagatggcg gcggaaatcg acgccaagct ggcggaagcc ggtctgggct gagccctgac
     3061 ggcaaagcgc ggggccgccg ttctccctga gcggccccgc agcttcgcct tctttccgca
     3121 catcgcaaat cgctttaggg taacgggtca gatgaccgca gccgcaaacc agattcatgc
     3181 gccggtgaag cagagccgct tcatgcggcg ggccctgccc tatatcctga gcctgccggc
     3241 gctgctgatg tgcatcggca tcctcattcc cttcttcacg gcggtctact actcgctcct
     3301 gcgctaccgc atgaacctgc cgcagatgcg cggcttcatc tggttcgaca actacatcaa
     3361 tttcttcgcc gacccgaagt tctggaacac ggtctatatc tcgctggtct atgcggcgct
     3421 caccgtcggg ctggaactga tcatcgggct cggcatcgcg ctcctcctgc agcggccgac
     3481 gcgtttcaac aacatcgcgt ccattcttct gctgcttcca ctcatgaccg cgcccgccct
     3541 ggctgcgctg atgtggaagc tcatgaccca tcccgacttc ggcatcctca gctacctgct
     3601 gacgctgatt ggcatcaccg acttcaaatg ggcctcggac ccctcgaccg cgctgctcac
     3661 cgtcgtgctg gtcgacatct gggtctacac gcccttcatc atgatcctgc tgctggcggg
     3721 cctgcggtcg ctgcccaagg cccccttcga ggcggcagcg ctcgacggcg tgccgcgcag
     3781 cttcgtgttc ttccgcatca cgctccccat gctgatgccc tacatcatca ctgccacgct
     3841 gttccgcctg ctcgactcga tccagcagtt cgacatcatc tatgcgatga cgcagggcgg
     3901 tccgggcgac aagctgctgg tcttccaggt ccaggcctat ctcgacttct tccaggccac
     3961 caatgtcgga cggtcggcgg cgctcatgat catcctctgg gcgatcacct atgcgctctc
     4021 caatgtcttc atcaagcaat ggctcaagct gcgcgaacgc gcgcatggtg cggggtaggc
     4081 catggaccac acatcattcc tcgagcgcct catccgcggc atcctgatca cgctggtgct
     4141 ggtgttcttc ctcttcccga tcgtctggat cctgctcatg tcgctgcaga ccaacgagac
     4201 gatcctgcgc attccgccgt cgctggtctt cacgccgatc ttcgacaact acaccgcgct
     4261 ggtcaccggc cagctgcgga ccgcggccgg cagcctgcag ctcaacttca tgcagaacct
     4321 gtggaactcg gtcctgctat cctcggcctc cgtgctcctg tcgctgatcc tcggcgttcc
     4381 cgccgcctat gccttcgcgc gcttcaagtt ccgcctgggc gaggacatcg cctttacgct
     4441 gctgtccttc cgcttcgccc cgccgctcct cgtgctgctg ccgctcagcc tctatttcaa
     4501 ggatcttggc ctgaccaaca cctacatcgg gctgatctgg gtctatcagc tcatctgcct
     4561 gccgctcatc ctgtggatcg tgcgcggcta tttcgaggac atcagtcccg acatcgaaca
     4621 tgcctatcgc atcgccggca acagctggtg gaagaccttc cgcaagatcg ccgtgccgct
     4681 ggcccggccc ggcatcgcgg cggcgggcct gctcgccttc atctttgcct ggaacaactt
     4741 cgtcttcgca ctcatcctgg catccgccga caagcagccg gtgactgtcg gcgcgctggc
     4801 cttcgtgacg gcctcaggca tccagtacgg acagattgca gccgccatcg tgctgtcggt
     4861 caccccgacg cttgcgctcg cgctctatgc ccagcgctat ctcgtcgagg gtctgtcgat
     4921 gggagcggtc aagggatgag ccagctctct ctcaaggcgc tgtcgaagtc atacggcgcc
     4981 ttcaaggtct tcgagggact cgacctgtcg gcggaggccg gcgagatcgt ggtggtcttc
     5041 ggcggttcgg gcaccggcaa gaccatcctg ctgcgcctca tcgcgggcgt cgaggagccg
     5101 accgcaggca gcatcctgat cggcggccat gacgtctccg acatcgcgcc ggaacaccgt
     5161 ggcgtcggca tggccttcca gaacttcgcg ctcttcccgc acatgtcggc ggccgagaac
     5221 atcgcaacac cgctgcgcgc caagggcgag tccgaacagg ccgtgcagtc cggcatcgcg
     5281 aaagtggcaa agctcctgaa gatcgaccac gtgctgggcc acgcgccgcg cgaattgtcc
     5341 aacggccaga agcagcgcac cgccctggcg cgcgcgcttg ccgcggaacc ccgcatcctg
     5401 ctgctcgacg atccgctgcg caacgtggat gcgaagctcc gtttcgagat gcggctcgag
     5461 ctgccgcgcc tgctgcgcca gagcggcgcc acggtcctct atgtgacgca ggactacaag
     5521 gaggccatgg cgctgggtga tcgcatcgcg gtcctcgcgg gcggcaaatt cgtccaggtc
     5581 gccacgcccg aggacattta tgtgaagccc gcctcgctga gcgtggcgcg gctgttcggc
     5641 gatccgtcga tcaatacggc cgagacggtg gtgaaggcgg gcggcaaggg cgcaggcctt
     5701 tccctcggcg gtacggaggt cgccatcgcc gcggaataca agggacttgc cggcaagcgc
     5761 gtcgtcttcg gcatccggcc ggaagccatc gagatcaagt caagcaagag cagcggcatc
     5821 ccggccgaga ttgtggcggt gacgccgctc aatgaacgca ccgtgctgct gctcaaggcc
     5881 gccgatggct gggaattcct cgcctcgctg cccgcctcgg caaaggcgcc cgagcccgga
     5941 acgaaagtcg cggccagctt cgcatcgtcc gatattcatt tcttcgacac ggcaagcggc
     6001 gagcggctcc atgggtgaac ttgtcatcag caaggtcgac aagatctatc gcggccgcaa
     6061 gacggcggtg catgccgtga aggcgctgga cctcacggtg aagccgggcg agatcgtggc
     6121 gcttcttggg tcatcgggct gcggcaagac ctcgacgcta cgcatgatcg cgggcttcga
     6181 agacgtgacc agcggcagca tcacgctgga cggcaagccg atccaccagc tccccccggc
     6241 gcagcgcggc gtggccatgg ccttcgaggc ctattcgctc tatccgcccc tcaccatcgg
     6301 cgagaacatc gccttcgcgc tgaaggcgtc gcggctgtcg gcggccgaac aggagagcaa
     6361 ggtccgcgcc atggccggga tgctggagat cgacggcatc ctgggcaagt atccctcctc
     6421 cgtctcgggc ggccagcagc agcgcgccag cctcggccgc gcgctggtgc gcaaggccgg
     6481 gctctatctg ctcgacgaac ccatgggcca gctcgaaccg cagctgcgcg ccatcctgcg
     6541 cggccgcatc aagcattatc tgcgcgagaa tgcctgcacc acgatcctgg tcacgcatga
     6601 ccagaccgag gccaatgcac tggcggaccg catcgccgtc atggaaggcg gcgaactgca
     6661 gcagtatgcg acaccggccg agctcaaggc gcggcccgcc aatctcttca ccggaacctt
     6721 catcggcgag ccgccgatga atgtcttccc cgccaccctg tcgatcaatt caaggactgt
     6781 gagcttcgat atcgcgggcg gtgccgcgct atcctacccc gcagccgagc tttccaggcc
     6841 cgtgcgg
//
LOCUS       DBNGVR010000221         3623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3224724, whole genome shotgun sequence.
ACCESSION   DBNGVR010000221 DBNGVR010000000
VERSION     DBNGVR010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3224724"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="aroC"
                     /locus_tag="AC16A0_04870"
     CDS             complement(<1..896)
                     /gene="aroC"
                     /locus_tag="AC16A0_04870"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3104697.1"
                     /translation="MSYNTFGHLFRVTTFGESHGPAIGCVVDGCPPNIPLTAAEIQAY
                     LDKRRPGQSRFTTQRQEPDQVKILSGVFEDERTPGQVTTGTPIMMLIENVDQRSKDYG
                     DIRDTFRPGHADFTYQAKYGIRDYKGGGRSSARETAARVAAGAVARKIIPGVTIRGAL
                     VQMGPHRIDRTNWDWEEVHRKPFFAPDAKAAKFYEDYLDGVRKAGSSCGAVIEVTASG
                     VPAGWGAPLYAKLDQELAAAMMSINAVKGVEIGAGFAAAAFSGEENADEIRMRRGKPE
                     FTSNHAGGILGGISTGQDLVVR"
     gene            complement(906..1511)
                     /locus_tag="AC16A0_04875"
     CDS             complement(906..1511)
                     /locus_tag="AC16A0_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3104698.1"
                     /translation="MSKARPPIYFIRHGETDWNRQGLIQGWTDTPLNEMGHGQARAVA
                     QALAALPDLTSDYQFFVSPLQRACQTMGYLAETLALDPQQIAIDADVQELGFGVWEGK
                     AFWELKASPIYPALPEDRYFWRPDGGESYEDGHARIRRWLGKVDRPTVVVAHGAIGRC
                     LVAEIAGLGLRDLVKLAMRQGCYCRLQDRTAEWFDATSDAA"
     gene            complement(1504..2328)
                     /gene="fabI"
                     /locus_tag="AC16A0_04880"
     CDS             complement(1504..2328)
                     /gene="fabI"
                     /locus_tag="AC16A0_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3104699.1"
                     /translation="MSENKGLMAGKRGLIMGVANNRSIAWGIAKSCAAHGAELAFTYQ
                     GDALKKRVEPLAAEIGSRIVVPCDVTDMASLDATFETLARDWGKLDFLVHAIAFSDKD
                     ELTGRYVDTSAENFHKTMFISVYSFTAIAQRAEKLMNDGGSLLTLTYYGAEKWMPHYN
                     VMGVAKAALEASVRYMAADLGPKAIRVNAISAGPIKTLAASGIGDFRYILRWNEYNSP
                     LRRTVTIDEVGDSGLYLLSDLGRGVTGEILHVDAGYHIVGMKHPDAPDITVNGHGE"
     gene            2618..3250
                     /gene="pdxH"
                     /locus_tag="AC16A0_04885"
     CDS             2618..3250
                     /gene="pdxH"
                     /locus_tag="AC16A0_04885"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR3104700.1"
                     /translation="MSLSDISTLTASMGFAERNDPFGLFGDWMKEAEKAEPNDPNAMA
                     LATVDDEGLPNVRMVLLKGFDDRGFVFYTNFESQKGQEILSTMKAALGFHWKSLRRQV
                     RVRGLVEEVTPAEADAYFASRPRDSRIGAWASQQSRPLESRFALETAVAMNTARYAIG
                     EVPRPPYWSGFRVKPLSIEFWHDRPFRLHDRVVFRRPNETSAWTKARLYP"
     gene            3247..>3623
                     /locus_tag="AC16A0_04890"
     CDS             3247..>3623
                     /locus_tag="AC16A0_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104701.1"
                     /translation="MNTSPDSERKTLILTGASRGIGHATVKRFSTAGWRVITCSRQPF
                     PEDCPWDAGPDDHIVVDLASSTDTHAAIAAMKERLKAQGSRLHALVNNAAISPKKPGG
                     ARLNTIETSEDDWKTVFQINFFAP"
BASE COUNT          625 a         1153 c         1194 g          651 t
ORIGIN      
        1 aagcgcacca ccaggtcctg gccggtcgat attccgccca ggattccgcc ggcgtggttg
       61 gaggtgaatt ccggcttgcc gcggcgcatg cggatctcgt cggcattttc ctcgccggag
      121 aaggccgcgg cggcaaagcc agcgccgatc tcgacgccct tcacggcatt gatgctcatc
      181 atcgcggcgg ccagttcctg gtcgagcttg gcgtagagcg gcgcgcccca gccggccgga
      241 acgccgctgg cggtcacctc gatcaccgcg ccgcaggagg atcccgcctt gcgcacgccg
      301 tcgagataat cctcgtagaa cttcgccgcc ttcgcatcgg gcgcgaagaa gggtttgcgg
      361 tgcacttcct cccagtccca gttggtgcgg tcgatccggt gcgggcccat ctgcacgaga
      421 gcgcccctga tggtcacgcc cgggatgatc ttgcgcgcca cggcgccggc cgccacgcgg
      481 gccgccgtct cgcgcgccga ggagcggccg ccgcctttgt agtcgcggat cccatacttc
      541 gcctgatagg tgaagtcggc atggccgggg cggaacgtgt cgcggatgtc gccatagtcc
      601 ttggagcgct ggtcgacatt ctcgatcagc atcatgatgg gcgtgccggt ggtgacctgg
      661 cccggcgtgc gctcgtcttc gaagacgccc gacaggatct tcacctggtc gggctcctgg
      721 cgctgcgtgg tgaagcgcga ctgtcccggc ctgcgcttgt cgagataggc ctggatctcg
      781 gcagcggtca gcggaatgtt cggcgggcag ccgtcaacca cgcaaccgat ggcggggcca
      841 tggctttcgc cgaaggtggt gacgcggaac agatggccga aggtattgta cgacatgcgt
      901 cctgattagg ccgcatcgct ggtggcgtca aaccactcgg cggtgcggtc ctgcaggcgg
      961 caatagcagc cctggcgcat ggcgagcttg accaggtcgc gcaggccgag gccggcgatt
     1021 tccgcgacca ggcagcggcc gatggcgccg tgggcgacga ccaccgtcgg acgatcgacc
     1081 ttgccgagcc agcgccggat gcgggcatgg ccgtcctcgt agctttcgcc gccgtccggg
     1141 cgccagaaat agcggtcttc cggcagggcc gggtagattg gcgaggcctt gagttcccag
     1201 aaggccttgc cctcccatac gccaaaaccc agttcctgca cgtcggcgtc gattgcgatc
     1261 tgctgcgggt cgagcgccag ggtctctgcc aggtatccca tggtctggca ggcccgctgc
     1321 aacgggctga cgaaaaactg gtagtcagac gtgagatcag gaagagccgc aagcgcctgg
     1381 gcgacggccc tggcctggcc gtggcccatc tcgttcagcg gcgtgtcggt ccagccctgg
     1441 atcaggccct ggcggttcca gtcggtctcg ccgtgccgga tgaaatagat cggaggcctg
     1501 gccttactca ccgtggccgt tcaccgtgat gtcgggcgcg tcgggatgct tcatgccgac
     1561 gatgtgatag cccgcatcca catgcaggat ctcgccggtg acaccgcggc cgagatccga
     1621 caggagatag aggcccgagt cgccgacctc gtcgatggtg acggtgcggc gcaagggcga
     1681 attgtactcg ttccagcgca ggatgtagcg gaagtcgccg atgccggagg ccgcgagcgt
     1741 cttgatcgga ccggcggaaa tggcgttgac gcggatggcc ttggggccaa ggtcggcggc
     1801 catgtagcgc accgaagctt cgagcgccgc cttggcaacg cccatgacat tgtagtgcgg
     1861 catccacttt tcagcgccgt agtaggtgag cgtcagcagc gagccgccgt cgttcatcag
     1921 cttctcggcg cgctgggcaa tggccgtgaa ggagtagacg ctgatgaaca tcgtcttgtg
     1981 gaagttctcg gccgaggtgt cgacatagcg gccggtgagt tcgtccttgt ccgagaaggc
     2041 aatggcatgg accaggaaat ccagtttgcc ccagtctctc gccagcgtct cgaaggtggc
     2101 atcaagggag gccatgtcgg tgacgtcgca ggggaccacg atgcgcgaac cgatttcggc
     2161 ggccagcggc tcgacccgct tcttcagcgc gtcgccctga taggtgaagg cgagttcggc
     2221 gccatgcgca gcacaggact tggcgattcc ccaggcaatc gaccggttgt tcgcaacgcc
     2281 catgatcagg ccgcgctttc cagccatcag ccccttgttt tcgctcatga tttttcctgc
     2341 atttccaggc cggaaccgac cttccggcgc cggttaattg gcacaagtcc ggaaacaggt
     2401 ccactggaaa gaaaatgccg ttatcctgtc atcttgaggc gtcaaggccc caacattccg
     2461 gcagccttgc actggcggca agggtggtgg atcggattgt cacttcgttc cccggcgact
     2521 tcgcgggcgc gatggtctat gcctgttccg caaaacatgc cggtgctgcg ggagtcgcct
     2581 cccgatgtcc ggcccaagcg atggaaaacc tgaagccatg agtctgagcg acatctccac
     2641 actgacggca tcgatgggct tcgcagagcg caacgatccc ttcggccttt tcggggactg
     2701 gatgaaggag gccgagaagg ccgaacccaa cgatccgaac gccatggcgc tggcaaccgt
     2761 ggacgacgag ggcctgccca atgtccgcat ggtgctgctc aaggggttcg acgatcgcgg
     2821 cttcgtcttc tacacgaact tcgaaagcca gaagggccag gagattctgt ccaccatgaa
     2881 ggcggcgctg ggctttcact ggaagtcgct gcgccgccag gtgcgggtgc gtgggctggt
     2941 cgaagaagtg acgccggccg aagccgatgc ctatttcgcc tcgcgtccgc gcgacagccg
     3001 catcggtgcc tgggcctcgc aacagtcccg tccgctggag agccgcttcg cgctcgagac
     3061 ggcggtcgcc atgaacaccg cgcgctatgc catcggcgag gtgccgcgcc ctccctactg
     3121 gtcgggcttc cgggtcaagc cgctgtccat cgagttctgg cacgaccggc cgttccggct
     3181 gcatgaccgc gtggtcttcc gccgcccgaa cgaaacgagc gcctggacca aggcgaggct
     3241 ctatccatga acacgtcccc cgacagcgaa aggaagacgc tgatcctcac cggcgcatcg
     3301 cgcggcatcg gccatgcaac ggtgaaacga ttctccaccg ctggctggag ggtcatcacc
     3361 tgttcgcgac agccgttccc cgaggattgc ccctgggatg ccggccccga tgaccatatc
     3421 gtcgtcgatc ttgcctcctc gacggatacc catgcagcca tcgctgccat gaaggagcgc
     3481 ctgaaggcgc agggctcgcg gctgcacgct ttggtgaaca atgccgccat cagcccgaag
     3541 aagccgggcg gggcaaggct caataccatc gagacgagcg aggacgactg gaagacggtg
     3601 ttccagatca acttctttgc gcc
//
LOCUS       DBNGVR010000222         4583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1065061, whole genome shotgun sequence.
ACCESSION   DBNGVR010000222 DBNGVR010000000
VERSION     DBNGVR010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1065061"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="AC16A0_04895"
     CDS             complement(<1..1186)
                     /locus_tag="AC16A0_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3104702.1"
                     /translation="MSKSIYASLGLAPIINAKGPATRLSGGIMRPEVAAAMVEASQHC
                     VDIAELQAAASREIAKATGAEAGYVASGASACLLLATAACITELDPGRMARLPDTRGL
                     RNEVIMVRSQRNFYDHAVRAAGAFIVEVGLPDRYAGAGVRDAEAWEIEDAITEKTAAI
                     FYVADAAAQPPLRDICKLARRRKIPVIVDAAAQLPPQSNLKRFIRDGADLVAFSGGKA
                     LGGPQASGLLCGRRDLIMAAALQHLDLDVYWDMWTPPPTLIDKRRLKGAPQHGIGRSC
                     KAGKEEIAGLLTALRLFVAEGDKARHARWLALLQMVQSGLGRTKGVVPSLSGAEDTAA
                     VPSLHLSFQREGRAAEVALLLQSQSPAIHVDAFLRQQNTLAINPMCIDPGQVTALLHG
                     LRK"
     gene            complement(1183..4092)
                     /gene="polA"
                     /locus_tag="AC16A0_04900"
     CDS             complement(1183..4092)
                     /gene="polA"
                     /locus_tag="AC16A0_04900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3104703.1"
                     /translation="MKPGDHLYLIDGSGYIFRAYHALPPLTRKSDGLPVGAVQGFCNM
                     LWKLLKDTNAGQKPTHLAVIFDKSSKTFRNDIYPAYKAQRPEPPADLRPQFGLIKQAT
                     RAFNVPAIEQDNFEADDLIATYARQAVEAGATCRIVSSDKDLMQLIRPGVTLYDTMKD
                     RDVGEAAVLEKFGVTPDKVIDVQSLAGDSVDNVPGVPGIGVKTAAQLLAEYGDLETLL
                     ARASEIKQQKRRENLIAFAEQARISKRLVTLDDQVPVEHDVSDFAVDQPRASQLIGFL
                     KALEFTSFTKKVAGELDADMAAIEPAPVEVSFWPPEGGENMADGTKAEAPARPPRSAP
                     KAETVPPAPSASDDALKAIPFRHEDYATVTDLAALEGWIAEANTQGYLAVDTETDALD
                     SMQANLVGVSLATAPGKACYVPLGHKASGGGLFGGDAVPGQIEVMAALARLKPLLEDP
                     AVLKIGQNLKYDIEVLARNGIAVAPIDDTMLISYALESGDVGHGMDELSDRHLGHKPI
                     SFKEVAGSGKSAVSFDRVELRRATDYAAEDADVTLRLWLLLKPRLAAMGKRTVYETLE
                     RPLVPVLARMEMAGIIVDPDVLKRLSNEFATAAAAIETQVYELAGERFNIGSPKQLGD
                     MLYGRMGLPGGRKTATGAWSTDSDALEDLAGQGVELARRVLDWRQLSKLRSTYTDALP
                     GYINPQTGRVHTSYSMAATSTGRLASTEPNLQNIPVRTEEGRKIRAAFVAPPGSKLIS
                     ADYSQIELRVLAHVADIPQLKKAFADGIDIHAMTASEMFGVPVKGMDPMVRRRAKAIN
                     FGIIYGISAFGLANQLGISREEAGNYIKTYFTRFPGIRDYMEATKKAAREQGYVETIF
                     GRRCHFPRINSPNPSERAFLERAAINAPIQGAAADIIRRAMIRMDDALAHAKLGARML
                     LQVHDELIFEAIDAEVDATIEVVRRVMEKAPEPAVQLTVPLQVDARAAQNWEEAH"
     gene            complement(4079..>4583)
                     /locus_tag="AC16A0_04905"
     CDS             complement(4079..>4583)
                     /locus_tag="AC16A0_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104704.1"
                     /translation="LAPQVFRFAEAVAARPLRDVALALAAWAAINTAFVLQGWATLPG
                     VALALGFMGAAAVVTMGVMLSKTRLAEPIRYAGENSIVIYLAFFLFMAATRAVLLKLM
                     PGLDLGLVSLIVTAAGVTGPVLMFWAVRKTPLAFLFRRPHWARLASPGRRWHSAPHGK
                     TANSEAR"
BASE COUNT          710 a         1507 c         1573 g          793 t
ORIGIN      
        1 ccttgcggag tccgtggagc agggctgtga cttgtcctgg gtcgatgcac atgggattga
       61 tcgcaagcgt gttctgctgg cgcaggaacg catcgacatg aatggcgggc gattggcttt
      121 gcagcagcag ggccacttcg gctgcacgcc cctccctctg gaacgacaga tgcagggacg
      181 gcaccgcagc cgtatcctcc gcgccggaca agcttggaac cacacccttc gtgcgcccga
      241 gtccgctctg aaccatctga agcagggcca gccatctggc atggcgggcc ttgtcgcctt
      301 ccgcgacgaa gagcctgagg gccgtgagca gaccggcgat ctcctccttg cccgccttgc
      361 agctgcggcc gatgccatgc tgcggcgcgc ctttcagcct gcgcttgtcg atcagcgtgg
      421 gcgggggtgt ccacatgtcc cagtagacgt cgagatcgag gtgctggagc gcggccgcca
      481 tgatgaggtc gcgccggccg cacaggagac cgctggcctg cggaccgccc agcgccttgc
      541 cgccggagaa ggcaacgagg tcggcgccgt ctcggatgaa gcgcttgaga ttggactgcg
      601 gcggcagctg tgccgcggca tcgacgatca ccgggatctt gcggcggcgc gccagcttgc
      661 agatgtccct cagcggcggc tgcgcggcgg catcggccac atagaagatc gccgccgtct
      721 tctcggtgat cgcgtcctcg atctcccagg cttcggcatc gcgcacgcct gcaccggcat
      781 agcgatcagg cagtcccact tcgacgatga acgcacctgc cgcgcgcacc gcgtggtcat
      841 agaaattgcg ctggctgcgc accatgatca cttcgttgcg caggccgcgg gtatcgggaa
      901 gcctggccat gcggccggga tcgagctccg tgatgcaggc cgcggtggcg agcagcaggc
      961 aggccgaggc cccggaggcc acatagcccg cttctgcgcc cgtggccttt gcgatctcgc
     1021 gcgaggccgc ggcctgaagc tccgcgatgt cgacgcagtg ctgcgaagcc tcgaccatgg
     1081 ccgcggccac ctctggccgc atgatcccgc cggacaggcg cgtggccggc cccttggcat
     1141 tgatgatggg ggcaaggccc aggctggcgt agatactctt gctcaatgcg cctcttccca
     1201 gttttgcgcg gcgcgcgcat ccacctgcag cggcacggtg agttgcaccg ccggctccgg
     1261 cgccttctcc atcacccggc gcaccacctc aatggtggcg tcgacctcgg catcgatcgc
     1321 ttcgaagatc agctcgtcat gcacctgcag cagcatgcgc gcgccgagct tggcatgggc
     1381 cagcgcatcg tccatgcgga tcatggcacg gcggatgatg tcggcggccg caccctggat
     1441 gggtgcattg atggcggcac gttcgaggaa ggcgcgctcg gagggattgg gcgaattgat
     1501 gcgcgggaaa tggcagcgcc gcccgaagat ggtctcgaca tagccttgct cgcgcgccgc
     1561 cttcttcgtt gcttccatgt agtcgcggat gccggggaag cgcgtgaagt aggtcttgat
     1621 gtagttgccc gcctcctcgc ggctgatgcc cagctggttg gccagaccga aggccgagat
     1681 gccgtagatg atgccgaagt tgatcgcctt ggcgcgccgc cgcaccatcg ggtccatgcc
     1741 cttgaccggc acgccgaaca tttcgctcgc cgtcatggcg tggatgtcga tgccgtcggc
     1801 gaaggccttc ttcagctgcg ggatatcggc cacatgggcc agcacgcgca gttcgatctg
     1861 gctgtagtcg gcgctgatca gcttcgatcc gggcggcgcc acaaaggcgg cgcggatctt
     1921 gcggccttcc tcggtgcgca ccgggatgtt ctgcaggttg ggctcggtcg aggcgagacg
     1981 gccggtggag gttgccgcca tcgagtagct ggtgtggacg cggccggtct gcgggttgat
     2041 gtagcccggc agcgcgtcgg tataggtcga gcgcagcttg gacaattgcc gccagtcgag
     2101 cacgcgccgc gccagctcca cgccctggcc ggcgagatcc tccagcgcgt cggagtcggt
     2161 cgaccaggcg ccggtggccg tcttgcggcc ccccggcagg cccatgcggc catagagcat
     2221 gtcgcccagc tgcttgggcg agccgatgtt gaagcgttcg cccgccagct cgtagacctg
     2281 cgtctcgatc gcggccgccg ccgtggcgaa ctcgttcgag agccgtttca gcacgtcggg
     2341 gtcgacgatg atgccggcca tctccatgcg cgccagcacc ggcaccagcg gccgctccag
     2401 cgtctcgtag acggtgcgct tgcccatggc cgcgagacgc ggcttgagga gcagccagag
     2461 ccgcagcgtc acgtcggcgt cttccgccgc atagtcggtg gcgcggcgca gctccacgcg
     2521 gtcgaagctc accgccgact tgccggaccc ggccacctcc ttgaagctga tcggcttgtg
     2581 gcccagatgg cgatcggaga gctcgtccat gccatgtccc acatcgcccg actcgagggc
     2641 ataggaaatc agcatggtgt cgtcgatcgg cgcgactgcg atgccgttcc gcgccagcac
     2701 ctcgatgtcg tatttcaggt tctgtccgat cttcagcacc gccggatcct cgagcagcgg
     2761 cttgaggcgg gcgagtgctg ccatgacctc gatctgtccg ggcaccgcat cgccgccgaa
     2821 gagcccgccg cccgaggcct tgtggcccag cgggacgtag caggccttgc cgggtgcggt
     2881 ggccagcgac acgcccacca gattggcctg catcgaatcc agcgcatcgg tttcggtgtc
     2941 gacggcgaga tagccctggg tgttcgcctc ggcgatccag ccttcgagcg ctgcaagatc
     3001 ggtcaccgtc gcgtagtcct cgtggcggaa gggaatggcc ttcagcgcat cgtcgctggc
     3061 cgacggcgcg ggcggcacag tttccgcctt gggagcgctg cgtggcggcc gcgcgggtgc
     3121 ttccgccttc gtgccatccg ccatgttctc gccgccctcc ggcggccaga aggaaacctc
     3181 caccggcgca ggctcgattg ccgccatgtc ggcgtcgagt tcgccggcca ccttcttggt
     3241 gaagctggtg aactcaaggg ccttgaggaa gccgatgagc tgggaggccc tgggctgatc
     3301 gacggcgaaa tcgctgacgt cgtgttccac cggcacctgg tcgtcgagtg tcaccagccg
     3361 cttggagatg cgcgcctgct cggcgaacgc gatcaggttc tcgcgccgct tctgctgctt
     3421 gatctccgag gcgcgcgcca gaagggtttc gagatcgccg tattccgcga ggagctgtgc
     3481 ggctgtcttc acgccgatcc ccggcacgcc cggcacattg tccaccgagt cgcccgccag
     3541 cgactgcacg tcgatcacct tgtcgggcgt cacgccgaac ttttccagca ccgcggcctc
     3601 gcccacgtcg cggtccttca tcgtgtcgta gagcgtcacg ccggggcgga tcagctgcat
     3661 caggtccttg tcggaagaga cgatgcggca ggtggcgccg gcctccaccg cctggcgcgc
     3721 ataggtggcg atgagatcgt cggcctcgaa attgtcctgc tctatggcgg gcacgttgaa
     3781 ggcgcgggtc gcctgcttga tcagtccgaa ctgcgggcgg agatcggccg gcggctcggg
     3841 gcgctgcgcc ttgtaggcgg gatagatgtc gttccggaag gtcttcgacg acttgtcgaa
     3901 gatcaccgcg agatgcgtgg gcttctggcc cgcattggtg tccttcagca gcttccacag
     3961 catgttgcag aagccctgca ccgcgcccac cggcaggccg tcggacttgc gggtgagcgg
     4021 cggcagcgcg tggtaggcgc ggaagatgta gccggaaccg tcgatcagat agagatggtc
     4081 accgggcttc agagtttgct gtcttgccat gcggcgcact atgccaccgg cgtccgggcg
     4141 atgccagcct tgcccagtgc gggcggcgga acaggaaggc cagcggcgtc ttgcgcacgg
     4201 cccagaacat cagaacggga ccggtgacgc cggcggccgt gacgatgagc gagacgagtc
     4261 ccagatcgag tcccggcatg agcttcaaca gcacggcgcg ggttgccgcc atgaagagga
     4321 agaaggcgag atagatgacg atggagttct cgccggcata gcgaatgggt tcggcgagcc
     4381 gggtcttcga cagcatcacg cccattgtca ccaccgcggc ggcacccatg aagcccagcg
     4441 ccagcgcgac gccgggaagc gtggcccagc cctgcagtac gaaggcggtg ttgatcgcag
     4501 cccaggccgc aagggcgagc gccacgtccc tgagcggtcg cgccgcgacg gcttcggcga
     4561 agcggaacac ctgcggggcc agc
//
LOCUS       DBNGVR010000223         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5189216, whole genome shotgun sequence.
ACCESSION   DBNGVR010000223 DBNGVR010000000
VERSION     DBNGVR010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5189216"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="AC16A0_04910"
     CDS             <1..588
                     /locus_tag="AC16A0_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF993 family protein"
                     /protein_id="HIR3104705.1"
                     /translation="DMFDPALKGYWGSEGFEPAMETCLSAIAATVAKVDGIKISLLDD
                     QKEVVMRRRLPAQVKMYTGDDFNYPSLIAGDGAGFSHALLGIFDAIAPAASGALAALA
                     QGDRARYDVLMSPTVPLSRLIFRAPTQFYKTGIVFLAWLNGHQDHFVMVNGAQAMRPL
                     PYFTGIFRLADQAGLLRDPALAVRRMKQLLAVYGV"
     gene            588..1442
                     /locus_tag="AC16A0_04915"
     CDS             588..1442
                     /locus_tag="AC16A0_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011649911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3104706.1"
                     /translation="MRDLSRDLGACAINTATLGFQAPISAVIDAVARAGFGGIAPWRR
                     EIEGQDVAAVARQIREAGLTVPGYCRSTYIPAADRSGFRENIESNRRAISTAAELGSH
                     SFVMVVGSLPPGSKDIAAARSQVREACALLLEHGRAEGVRIALEPLHPVYAADRSCLS
                     LLSEALDLCEAIEGGVPEPWLGTCIDVYHVWWDPNLVRDIARAGAGRRIFGFHVCDWL
                     VPTEHPLNDRGMMGDGVIDIPAIRSMIEEAGYAGLVEAEIFSAENWWKRPMPEILATC
                     RDRFASAV"
     gene            1480..1917
                     /locus_tag="AC16A0_04920"
     CDS             1480..1917
                     /locus_tag="AC16A0_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR3104707.1"
                     /translation="MDAFESCIGQSVSCTKTVGESDVYLFAGITGDLSPNHVDEAAMS
                     KTAYGHRIAHGALLVGYMSRASTLICDRCGPLMATHFPVSLGYDRVRFLSAVLIGDTI
                     TVNYRITAADRGKSRTLAEVELHNQQGTLCAVAAHIMSWIART"
BASE COUNT          336 a          657 c          657 g          367 t
ORIGIN      
        1 gacatgttcg accccgccct gaaaggctac tggggcagcg agggcttcga acccgcgatg
       61 gaaacctgcc tctcggcgat cgcggccacg gtggccaagg tcgatggcat caagatctcg
      121 ctgctggatg accagaagga ggtcgtgatg cggcgccggc ttccggcaca ggtgaagatg
      181 tacacgggcg acgacttcaa ctacccctcg ctgatcgccg gcgacggcgc gggattctcg
      241 catgcgctgc tcggcatctt cgatgccatt gcgccggccg cctcgggcgc cctcgccgcc
      301 ctggcgcagg gcgaccgtgc ccgctacgac gtgctgatga gtccgaccgt gccgctgtcg
      361 cgcctcatct tccgcgcgcc cacgcagttc tacaagacgg gcatcgtctt cctggcctgg
      421 ctcaacggcc atcaggatca cttcgtgatg gtcaatggcg cgcaggccat gcggcccctg
      481 ccctatttca ccggcatctt ccggctggcg gaccaggcgg gcctgctgcg cgaccccgcg
      541 cttgcggtga ggcgcatgaa acagctgctt gcggtctacg gcgtctgatg cgcgacctct
      601 cccgcgacct cggggcctgc gcgatcaata ccgcgactct cggattccag gcccccatct
      661 cggcggtgat cgatgccgtg gcccgggccg gcttcggcgg catcgcgccg tggcgccgcg
      721 agatcgaagg ccaggatgtc gccgccgtcg cgcggcagat ccgcgaggct ggactcactg
      781 tgccgggata ctgccggagc acctatatcc cggctgcgga tcgcagcggc ttccgcgaga
      841 atatcgagtc caatcgccgc gccatttcca cggcggccga gcttggctcc catagcttcg
      901 tcatggtggt gggcagcctg ccaccgggct cgaaggatat cgctgcggcc aggtcccagg
      961 tccgagaggc ctgcgccctg ttgctcgagc acggccgggc ggaaggcgtc cgcatcgcgc
     1021 tcgagcccct gcatccggtc tatgccgcag accgttcctg tctttccttg ctgtccgaag
     1081 cgctcgacct ctgcgaggct atcgaaggcg gcgttccgga accctggctc ggcacctgca
     1141 tcgatgtcta ccacgtatgg tgggacccga atctcgtgcg cgacatcgcg cgcgccggcg
     1201 caggccggcg catcttcgga ttccacgtct gcgactggct ggtaccgacc gagcatccgc
     1261 tgaatgaccg tggcatgatg ggcgatggcg tcatcgacat acctgccatc cggtccatga
     1321 tcgaggaagc aggctatgct ggtctcgtcg aggccgagat cttctcggcc gaaaactggt
     1381 ggaagcgccc catgcccgaa atcctcgcaa cctgccgtga ccgctttgcc tcggccgtct
     1441 agagtgtgcc aacctacgca ggaagaggag agcttgacgg tggacgcatt cgagtcatgc
     1501 atcgggcagt cggtgtcctg cacaaagacg gtgggcgaga gcgatgtcta cctctttgca
     1561 gggatcacgg gcgacctgtc gccgaaccat gtggacgagg cggcgatgtc gaagacggcc
     1621 tatggccatc gcatcgcgca tggtgccctg ctggtgggct acatgtcgcg ggcgtcgacg
     1681 ctgatctgcg acaggtgcgg gccattgatg gcgacgcact ttccggtctc gctcggctat
     1741 gatcgggtcc gctttctcag tgcggtgctg atcggtgaca cgatcaccgt caactaccgg
     1801 atcaccgcag cggatcgcgg caagagccga acgctggcgg aggtggaact gcacaatcag
     1861 caaggcactc tctgcgccgt tgcagcccac atcatgagct ggattgcgcg gacatgacag
     1921 gcgcggcgaa ggcgtccggc ttgccaatcg ggatgcaggt cactattcgt aaccatacgg
     1981 ttaactgagt gagaaaatga cgggcatggc agcgcag
//
LOCUS       DBNGVR010000224         4387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1654278, whole genome shotgun sequence.
ACCESSION   DBNGVR010000224 DBNGVR010000000
VERSION     DBNGVR010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1654278"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="AC16A0_04925"
     CDS             complement(<1..1388)
                     /locus_tag="AC16A0_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR3104708.1"
                     /translation="MAGIFDEMQGEGGAVRDPYGRIEQWLRTLGKKDVAHALKEAEAM
                     FRRQGITFAVYGESEASERLIPFDIIPRVFAAAEWRKLSAGIEQRVRALNAFIHDLYH
                     RQEILRAGRIPQEIIIQNDAFVPEMIGVNPARGIYAHIIGIDIVRVSENEFYVLEDNC
                     RTPSGVSYMLEDREAMMYLFPDLFARHRVAPVENYPAMLRKTLESVAPPACKGEPTVV
                     ILTPGIHNSAFFEHAFLADEMGVELCEGGDLFVSDGYLYMRTTQEPKRVDVVYRRIDD
                     AFLDPLAFRPDSAIGVPGIIDLYRAGRVTLVNAPGTGIADDKAIYTYIPDVIEFYTGE
                     KPLLKNVPTWRCADPGDLAYVLEHLPELVVKEVHGSGGYGMLVGPTSSRKEIEAFRAK
                     LKAKPRNYIAQPTLALSAVPTFTSSGVAPRHVDLRPFVLSGDQVRITPGGLTRVALKK
                     GSLVVNSSQGGGT"
     gene            1678..3063
                     /locus_tag="AC16A0_04930"
     CDS             1678..3063
                     /locus_tag="AC16A0_04930"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3104709.1"
                     /translation="MTIHAKNINVAEVVKADIEHHFHPFTDHKSFHAEGGPRVMTQAD
                     GIWIWDANGNKLLDGMSGLWCVNVGYGRKELAEVAYRQMQDLPYYNTFFKTTTVPATE
                     LAAKISSVMPERFKHIFFTNSGSESNDTIVRFVRHYWKLKGKPYRQHFIARTRGYHGS
                     TMVAANLGGMQGMHEQVGTEFPGFHHVQQPYWYDFGGDKTPEDFGLEAAAAVEKKILE
                     VGPDNVAAFIGEPIQGAGGVLIPPTTYWPEVQRICRKYGVLIISDEVICGFGRTGNWW
                     GFQSLGFEPDIVPMAKGLSSGYLPIGAVAFADHIIKDFFELGGEFYHGMTYAGHPVAA
                     AVALKNIEILEQEKLVDRCRELGPYFAQALASLRDHPIVGETRSMGLIGAIELSSNKA
                     KRSRFKDSGRVGTKCRDYCFKNGLIMRACWDTMVIAPPFVITKKEIDELVRLARVALD
                     ATYADVRSEME"
     gene            3201..4280
                     /locus_tag="AC16A0_04935"
     CDS             3201..4280
                     /locus_tag="AC16A0_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104710.1"
                     /translation="MSAYRPKFRPNRRMVLKGTASLAAGLTILPRFSMAEEEKKLNFY
                     NWDTYIGENTLADFNKATGIEVRLDLFADNDELFAKLKGGNPGYDVIVPTNDNLERMI
                     KANMIMPLDHAKIPNMANIDEPFKDADFDPGRKFSIPYMWGTLGIGYRKSKVAEPVDS
                     WKILLDSDQYAGNIALLGDASNCLGATLKYLGYSWNSTNIEELKKAEALLIAQKKNIK
                     VFADDNGQDLLASGEVDACMEWNGDIKQVITEDEDLAYVVPTEGSLLWQDTLAIPMGA
                     PHPMNAHAFINFVMGAEEGKHIAETILYATANRAAKDLMPEDYRNNPVIFPSAEIIAK
                     CEPGLYLGEEATKVRDEIWTRVLAA"
BASE COUNT          892 a         1373 c         1323 g          799 t
ORIGIN      
        1 gtgccgcccc cctggcttga gttcaccacg agcgagccct ttttcagtgc cacgcgcgtc
       61 aggccgcccg gcgtgatccg cacctgatcg cccgacaaca cgaagggccg gaggtcgacg
      121 tggcggggcg cgacgccgga actggtgaag gtcggcaccg ccgacaaggc cagggtcggc
      181 tgggcgatgt agttgcgcgg cttggccttc agcttggcac gaaatgcctc gatctccttg
      241 cgcgaagaag ttgggccgac cagcattcca tagccgccgg aaccatggac ctccttgacg
      301 acaagctcgg gcaggtgttc gagaacatag gcgagatcgc cggggtcggc gcagcgccag
      361 gtcggcacgt tcttcagcag cggcttctcg ccggtgtaga actcgatcac gtcgggaatg
      421 taggtataga tcgccttgtc gtccgcgatg ccggtgcccg gcgcgttgac caacgtgacc
      481 cggcccgcgc ggtagaggtc gatgatgccg ggaacgccga tggccgagtc cggccggaag
      541 gcgagggggt cgagaaaggc atcgtcgatc cggcggtaga cgacatccac ccgcttcggt
      601 tcctgggtcg tacgcatgta gagatagcca tcggagacga aaaggtcgcc accctcgcag
      661 agttccacgc ccatctcgtc ggcgaggaag gcatgttcga agaaggccga gttgtggatg
      721 ccgggtgtca ggatcaccac cgtcggctcg cccttgcagg caggcggggc cacgctttcc
      781 agcgtcttgc gcaacatggc ggggtaattc tcgacaggtg ccacgcgatg gcgcgcgaag
      841 aggtccggga agaggtacat cattgcctcg cggtcctcga gcatgtagga cacgcccgaa
      901 ggcgtgcggc aattgtcctc gagaacatag aactcgttct cgctcacccg gacgatatcg
      961 attccgatga tatgggcata gatgccgcgg gccgggttca cgccgatcat ctccggcacg
     1021 aaggcatcgt tctggatgat gatctcctgc gggatgcggc cggccctcag gatttcctgg
     1081 cggtggtaga gatcatggat gaaggcgttc agcgcccgga cccgttgctc gatcccggcc
     1141 gacaacttcc gccactcggc ggcggcgaag acccgcggga tgatgtcaaa ggggatgagg
     1201 cgctccgagg cttcgctctc gccatagacc gcgaaagtga tgccctggcg ccggaacatg
     1261 gcctcggcct ccttcagggc atgggccaca tccttcttgc ccagcgtcct gagccattgc
     1321 tcaatgcggc cgtagggatc ccggacggct cccccttcac cctgcatttc atcgaagatc
     1381 ccggccatcc cccgccctat gctgcattgc aataagatga caattcagga aagctggcaa
     1441 gcgaaacctg ccgtccgagt caatttgttg caagtttgag ccgaaaatga taaccggagc
     1501 tatgtgtttc cacatgacca cctctggcat tctcctgccg gtctggaccc gcacggtcca
     1561 tgacgaggtg atgctgcatt gcgccaggat cggcttcaag ccctagtttc tctacgtttt
     1621 gtaagttgaa tcaggccctt gcggggccca ttgctgcatg ttgatttgga gaacgaaatg
     1681 acgatccacg ccaagaacat caatgtcgcc gaagttgtga aggccgacat cgaacaccat
     1741 ttccatccct ttacggacca caagtcattc cacgcggaag gcggcccgcg ggtgatgacc
     1801 caggccgacg gaatctggat atgggatgcc aacggcaaca agctgctcga cggcatgtcg
     1861 ggcctgtggt gcgtcaatgt cggatacggc cggaaggagc tggccgaggt cgcctatcgg
     1921 cagatgcagg acctgcctta ctacaacacc ttcttcaaga cgaccacggt gccggccacc
     1981 gagctggccg ccaagatctc ctcggtgatg cccgagcgct tcaagcacat cttcttcacc
     2041 aactcgggtt ccgaatccaa cgacaccatc gtgcgctttg tgcgccacta ctggaagctc
     2101 aagggcaagc cctaccgcca gcacttcatc gcccgcacgc ggggctatca cggctccacc
     2161 atggtggcgg caaacctcgg cggcatgcag ggcatgcacg agcaggtggg caccgagttc
     2221 ccgggcttcc atcatgtgca gcagccttac tggtatgact tcggcggcga caagacgccg
     2281 gaggacttcg gcctcgaggc ggcggctgct gtcgagaaga agatcctgga agtcggcccc
     2341 gacaatgtcg ccgccttcat cggcgaaccg atccagggcg ccggcggcgt gctcatcccg
     2401 cccaccacct actggccgga agtgcagcgc atctgccgca agtatggcgt gctgatcatc
     2461 tctgacgaag tgatctgcgg ctttggccgc accggcaact ggtggggttt ccagtcgctg
     2521 ggcttcgagc cggacatcgt tccgatggcc aagggcctgt cgtcaggtta tctgccgatc
     2581 ggtgccgtgg ccttcgccga ccacatcatc aaggacttct tcgagctggg cggcgaattc
     2641 tatcacggca tgacctatgc cggtcacccg gtcgccgccg ccgtggccct caagaacatc
     2701 gagatcctgg agcaggaaaa gctggtcgac cgttgccgcg agcttggacc ctatttcgcc
     2761 caggcccttg cctcgctgcg cgaccatccg atcgtgggcg agacccggtc catgggcctc
     2821 atcggcgcca tcgaactgtc cagcaacaag gccaagcgca gccgcttcaa ggattccggc
     2881 cgcgtcggca ccaagtgccg ggactactgc ttcaagaatg gcctgatcat gcgggcatgc
     2941 tgggacacga tggtcattgc gccgccattc gtgatcacca agaaggaaat cgacgaactc
     3001 gtgcgccttg cgcgggttgc ccttgatgca acctatgcgg acgtgagatc cgagatggag
     3061 tgagttagga tttatcgagc gaattcgccg actgccgtga tgggggcctg actgggtccg
     3121 ggcggcagcc ggcgcaccaa ccggcaccaa aatacgccag agagcgacgt gccagacaac
     3181 acctcagagg ggtaacaaac atgagtgctt atcgtccgaa gttccggccg aaccggcgca
     3241 tggtgctgaa gggcactgct tcgctggccg ctggcctcac catcctcccg cgcttctcga
     3301 tggccgaaga ggaaaagaag ctcaacttct acaattggga tacctatatc ggcgagaaca
     3361 cgctcgccga cttcaataag gcgaccggca tcgaggtgcg cctcgatctc ttcgccgaca
     3421 atgacgagct ctttgccaag ctcaagggcg gcaaccccgg ctatgacgtc atcgttccga
     3481 ccaacgacaa tctggaacgt atgatcaagg ccaacatgat catgccgctg gatcacgcga
     3541 agatcccgaa catggccaac atcgacgagc ccttcaagga tgccgacttc gatccgggcc
     3601 gcaagttctc gattccctac atgtggggaa cgctgggcat cggctaccgc aagtccaagg
     3661 tggcagagcc ggtcgacagc tggaagatcc tgctcgactc cgaccagtat gccggcaaca
     3721 tcgcgctgct gggcgacgcc tcgaactgcc tcggtgccac gctgaagtac ctcggctatt
     3781 cctggaactc gaccaacatc gaggaactca agaaggctga agcgctgctc atcgcccaga
     3841 agaagaacat caaggtcttc gccgacgaca acggccagga tcttctggct tctggcgaag
     3901 tggatgcctg catggaatgg aacggcgaca tcaagcaggt catcaccgaa gacgaggacc
     3961 tcgcctatgt ggtgccgacc gagggctcgc tgctctggca ggacacgctc gccatcccga
     4021 tgggcgctcc gcatccgatg aacgcccatg ccttcatcaa cttcgtgatg ggtgcagaag
     4081 agggcaagca catcgccgag acgatcctct acgcgaccgc caacagggcc gccaaggatc
     4141 tcatgccgga agactaccgc aacaatccgg tgatcttccc ctcagccgag atcatcgcca
     4201 agtgcgaacc cggcctctat ctcggcgaag aggccaccaa ggtccgcgac gagatctgga
     4261 ccagggttct cgcggcctga gccacaacgc tttgcgacgg gcggcgggat catccgccgc
     4321 ccggttctct ttccctgact gatcatggtc aagagcctgc atccgccacg tctgccgctg
     4381 gaagtgt
//
LOCUS       DBNGVR010000225         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3619018, whole genome shotgun sequence.
ACCESSION   DBNGVR010000225 DBNGVR010000000
VERSION     DBNGVR010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3619018"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC16A0_04940"
     CDS             complement(<1..447)
                     /locus_tag="AC16A0_04940"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3104711.1"
                     /translation="MTTKISIHDLSKLSIVERNRLLQRTESDLSSFEEKVKPIIEAVR
                     TEGDAALARFARQFDKAPVEAEAIAATEADFARAEKTLDPRVKEALEFAAVSIRRFHE
                     DQKPEEMWMHEIRPGVFAGDRMSPIPSVACYVPRGKGAFPSAVMMTT"
     gene            complement(459..1217)
                     /locus_tag="AC16A0_04945"
     CDS             complement(459..1217)
                     /locus_tag="AC16A0_04945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104712.1"
                     /translation="MPLEIFSLAGRRALVTGASRGIGRAIALGLAGAGADLALAARSG
                     DALKSVADDVARLGRKAVTIAMDVSEVSDCRRAAADAADALGGLDILVNNAGVEQVCP
                     SLEVEEDLWDRILDTNLKGAFFMAQASARLMSGGAILNLCSLTSERGIPTAVPYGASK
                     TGLLGMTRALAVEWAPRGIRVNALEPGYFRTDLTEVFYRNDAWQKAMLANIPLQRFGE
                     LDDLVGASIFLCSDAARYITGACLPVDGGTLASI"
     gene            complement(1264..1926)
                     /locus_tag="AC16A0_04950"
     CDS             complement(1264..1926)
                     /locus_tag="AC16A0_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3104713.1"
                     /translation="MFESLLTGEALWAFAQVVMIDLVLAGDNAVVIGLAAAGLPKDQR
                     NKAIFVGIIAATVLRIAFASVATQLLEILGLLLIGGILLLWVCWKMWRELMQARSQQM
                     GAEAVTGVDLNADGTVAAGAPRKTFRQAAWQIIVADVSMSLDNVLAVAGAAREHPMVL
                     VFGLALSIALMGFAASFIAKLLQKHHWIAYVGLAIILYVALEMIYRGAMELYPVAMQV
                     AS"
     gene            complement(2013..>2411)
                     /locus_tag="AC16A0_04955"
     CDS             complement(2013..>2411)
                     /locus_tag="AC16A0_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR3104714.1"
                     /translation="TVKASHEYASDIVNSVWTGEPSVIYGNLRNNGCIPSLPADCAAE
                     VACLVDDRGIQPTYVGSLPPQLTALMRTNINVQELTVAALLTENREHIYHAAMLDPHT
                     AAELDLDQIWSLVDQLIDDHGNWLPAWVRR"
BASE COUNT          462 a          755 c          801 g          393 t
ORIGIN      
        1 ggtcgtcatc atcacggccg agggaaaggc ccccttgccg cgcggtacgt agcaggcaac
       61 cgacgggatg ggcgacatgc gatcgcccgc aaagacgccg gggcggatct cgtgcatcca
      121 catttcctcc ggcttctggt cttcatggaa acggcgaatt gaaacggcgg caaactccag
      181 cgcctccttc accctgggat cgagcgtctt ctcggcccgg gcgaaatcag cctcggtggc
      241 ggcaatggct tcggcctcga ccggtgcctt gtcgaactgg cgcgcaaagc gggcgagcgc
      301 cgcgtcgcct tcggtccgga cggcctcgat gatcggcttc accttctcct cgaaactgga
      361 aaggtccgac tcggtgcgct gcagcaggcg gttgcgctcg acgatcgaga gcttcgagag
      421 gtcgtggatg ctgattttcg tggtcatggg atcttccatc aaatcgaggc gagcgtgccg
      481 ccgtccacag gcaggcaggc accggtaatg tagcgcgcgg catcggaaca caggaatatg
      541 ctggcgccga ccagatcatc cagctcgccg aagcgctgca gcggaatgtt tgcaagcatc
      601 gccttctgcc aggcgtcgtt gcggtagaaa acttcggtga gatcggtgcg gaagtagccc
      661 ggctcgagcg cattgacgcg aatgccgcgc ggcgcccact ccaccgccag ggcgcgggtc
      721 atgcccagga ggccggtctt ggacgcgcca tagggcacgg cggtgggaat gccgcgctcg
      781 gaggtcagcg agcagaggtt gaggatcgcc ccgccggaca tgagcctggc ggaggcctgg
      841 gccatgaaga aggctccctt caggttggtg tcgaggatgc ggtcccacag gtcctcttcc
      901 acctcgagcg aggggcagac ctgttcgacg ccggcattgt tgacaagaat gtccagtccg
      961 cccagcgcat ctgctgcatc agccgcggca cggcggcaat cggacacctc cgagacatcc
     1021 atcgcaatgg tgactgcctt gcggcccagg cgggccacgt catcggcgac ggacttgagc
     1081 gcgtcgccgg accgcgcggc cagggccaga tcggcgcccg ctcccgccag cccaagggca
     1141 atggcacggc caatgccgcg gctggcaccc gtgaccaggg cacgccgtcc ggcaagcgag
     1201 aagatttcca ggggcatgac gggcggattc acccgagccg gcgggcagtg tcaagcgccg
     1261 gcctcaggag gccacctgca tggcaaccgg atagagctcc atggcgccgc gatagatcat
     1321 ctccagggcc acatagagga tgatggcgag gccgacatag gcgatccagt gatgcttctg
     1381 cagcagcttg gcgatgaatg aggccgcaaa gcccatgagc gcgatggaaa gcgccaggcc
     1441 gaagacgaga accatgggat gctctcgcgc ggcgccggca acggcaagaa cattgtccaa
     1501 cgacatcgag acatcggcca cgatgatctg ccaggctgcc tggcggaagg tcttgcgcgg
     1561 cgctccggcc gccaccgtgc catccgcatt gagatcgacg ccggtgaccg cttcggcacc
     1621 catctgctgg gatcgcgcct gcatgagctc gcgccacatc ttccagcaga cccagagcag
     1681 cagaatgccg ccgatgagca gaaggccgag aatttccagc agctgggtgg cgacgctggc
     1741 aaaggcgata cgcagcacgg ttgccgcgat gatgccgacg aagatcgcct tgttgcgctg
     1801 atccttgggc aggccggccg ccgcgaggcc gatgaccacc gcattgtcgc ccgccagcac
     1861 aaggtcgatc atgacaacct gggcaaaggc ccagagcgct tcacccgtca gcagcgattc
     1921 aaacatcccg tcttcccaat ggttgcagga ccccgccctg cggcctaggg cctacaatag
     1981 atggcgttgg acccgcaagg gcaaatgcag cttcagcggc gaacccaggc cggcagccag
     2041 ttgccatggt catcgatgag ctggtccact aggctccaga tctggtcgag gtccagctcc
     2101 gccgccgtat gcggatcgag catggcggcg tgatagatat gctcgcggtt ttcggtcagg
     2161 agggctgcga cggtaagctc ctgcacgttg atgttggtcc gcatcagcgc ggtgagctgc
     2221 ggcggcaggc tgccgacata ggtgggctgg atgccgcgat cgtcgacgag gcaggccact
     2281 tccgctgcgc aatccgcagg caacgagggt atgcagccat tgttgcggag attgccatag
     2341 atcaccgagg gttctccggt ccagacggag ttcacgatat cggaggcata ttcgtggctg
     2401 gccttcacgg t
//
LOCUS       DBNGVR010000226         3045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4994721, whole genome shotgun sequence.
ACCESSION   DBNGVR010000226 DBNGVR010000000
VERSION     DBNGVR010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4994721"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..989
                     /locus_tag="AC16A0_04960"
                     /pseudo
     CDS             1..989
                     /locus_tag="AC16A0_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315386.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
     gene            1083..1985
                     /locus_tag="AC16A0_04965"
     CDS             1083..1985
                     /locus_tag="AC16A0_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104715.1"
                     /translation="MTGREQSYGTGVTFVLLATLGWSLSGIFVRLMPELDGWQINTWR
                     GFWMSVGLLVYLVAIYGRDTLRRFLAIPLIAMCVSAGFFSIGSTLYVTSLTLVSTATI
                     SVIGASSPIFTGLLSPWVTGERPGLAAWIAAALAMVGVGIIAWDGLGGGKLVGILVSI
                     CVPISFAIQTLALRRYRGIDMVPAICVGGFVTFFAAGLLGFLAGHAGGGFDVGPREVL
                     LLAIMGPLQLSIPLIFYAKGAKSVPAVTLSLIVMLDAVINPLWPWLFVGEVPEPTAFI
                     GGAIIIGAVIISIFGGRFIARQRA"
     gene            complement(1963..2553)
                     /locus_tag="AC16A0_04970"
     CDS             complement(1963..2553)
                     /locus_tag="AC16A0_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HIR3104716.1"
                     /translation="MIESVREWLASRWVAGALFMGLALLAIVPILMGSWPLALLLIFL
                     HAPVYMLHQVEEHGRDRFRTYVNQKVFGGVEALTTDAVLWINIPCVWGLTLVALYAAW
                     LIAPGWGLMAPWLIIVNAITHIANAIKRREYNPGLLTSIFLFLPLGFATLHVIPGTLG
                     QTLFALGLSLLVHVMIVVTVFHHARVLRNLRPAAAR"
     gene            complement(2678..>3045)
                     /locus_tag="AC16A0_04975"
     CDS             complement(2678..>3045)
                     /locus_tag="AC16A0_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR3104717.1"
                     /translation="VIPAPAEVDALVARIPKGMLATLATLRQSLAAQHGATIACPVTT
                     GIFMGLVARAAEEREMMGAKRVTPWWRVLKTDGQLNPRMPGGLAEHRRRLEAEGFAIV
                     KAGRSGLMVESFEQRLARL"
BASE COUNT          515 a          998 c          964 g          568 t
ORIGIN      
        1 tccaggacat gtcggccttc gccaagatgg aagtatcggg acctggtgcg cgtgaatggc
       61 tggactcgat cctcgccaat cgaatcccga agaagatggg ccgcgtggcg ctctgccacc
      121 tgctcacgcc gcgcggcgga gtgcgcgccg agttcaccgt ctacgaatgg gcgcccggcc
      181 gcttctatct cgtctccgcc ggcgcctatg agcgtcacga ccacgacgta ttgcgcaagc
      241 tggcgcccac cgatggctcg gtgcgcctca atgccatcac cacccggctc ggcgtgctcg
      301 tcctcgctgg tcccaacagc cgcaaggtcc ttcagaagct gacagatgcc gacctctcca
      361 acgaggcctt cccctggctc tcgggctagg agatcagcgt ggggacgacc acggcgcatg
      421 cgctgcgcgt gaacttcgtg ggcgaactcg gatgggagtt ccatcacccg atcgagcagc
      481 aggtcgccct cttcgaccag ctgatggcgg cgggcaagga attcggcatc cgtccctatg
      541 gcatcaaggc gatgtcggcg ctgtcgatcg agaagagcta ccgcctggtg ccgcgcgaac
      601 tctcgatcga gtactccgcc tatgaatcgg gcctcgaccg cttcgtgcat cccaacaagg
      661 gcagcttcat cggccgcgac gcgctggtgg agggcaaggc gaaagggctg aactggaact
      721 tcgtcaccat ggaagtccat ggcatcacgg atggcgactc ggatgcccgc gggtccgaac
      781 cgctctatca caagggcaag ctgatcgggc gggcgaccaa tggcggcttc ggtttccggg
      841 tcaacaagtc cctcgcgctg ggcatggtcc agcctgacgt cgcggccgtg ggcaccgaac
      901 ttgaaatcaa gattctcggc aagctgttca aggcgaccgt gatcccggaa agcccctacg
      961 atcccgacaa tgccgcgctg agggcctgaa acagctgaca gcattttgaa gggccccatg
     1021 cgatcttgcc cgatccatgg tccctgttcc tttgctaggc tggcctgcga atcggggacg
     1081 ggatgacggg gcgggagcag agctacggga cgggtgtcac cttcgtgctg ctggccacgc
     1141 tgggctggag cctgtccggc atcttcgtcc gcctcatgcc cgaactcgac ggctggcaga
     1201 tcaatacctg gcgcggcttc tggatgtcgg tcggcctgct ggtctacctg gtcgccatct
     1261 acgggcgcga cacgctgcgc cgtttcctgg ccattccgct catcgccatg tgcgtttcgg
     1321 ctggcttctt ctccatcggc tcaaccctct acgtgacctc gctgacgctg gtcagcacgg
     1381 cgaccatctc ggtgatcggc gcttcgtcgc cgatcttcac cggccttctc agcccctggg
     1441 tgacggggga acgccccggt ctggcggcgt ggatcgccgc ggcgcttgcc atggtgggcg
     1501 tcggcatcat cgcatgggat ggtcttggcg gcggcaagct ggtgggaatc ctggtctcga
     1561 tctgcgtgcc gatctccttt gccatccaga ccctggcgct gcgccgctac cgcggcatcg
     1621 acatggttcc tgccatctgc gtcggcggct tcgtgacctt ctttgccgcg ggtcttctgg
     1681 gcttcctcgc cgggcatgcg ggtggcggct tcgatgtcgg cccgcgcgag gtgctgttgc
     1741 tggccatcat ggggccacta cagctgtcga tcccgctcat cttctacgcc aagggggcaa
     1801 agtcagtgcc cgccgtcacc ctgtccctca tcgtcatgct ggatgccgtg atcaatccgc
     1861 tctggccctg gctgttcgtc ggcgaggtac cggaaccgac cgccttcatc ggtggcgcca
     1921 tcatcatcgg cgcggtcatc atcagcatct tcggcggccg cttcatcgcg cggcagcggg
     1981 cctgaggttg cgcaataccc ttgcgtgatg gaagacggtg accacgatca tcacatgcac
     2041 cagcagcgaa aggcccagcg cgaagagcgt ctgtccgagc gtgcccggaa tgacatggag
     2101 cgtcgcgaag cccagcggca ggaagaggaa gatgctggtg agcaggccgg ggttgtattc
     2161 gcggcgcttg atggcattgg cgatatgagt gatggcgttg acgatgatca gccagggggc
     2221 catgaggccc cagcccggcg cgatcagcca ggcggcataa agcgccacca gcgtcaggcc
     2281 ccagacgcag gggatgttga tccacagcac tgcgtccgtc gtgagtgcct cgacgccgcc
     2341 gaagaccttc tggttcacgt aggtgcggaa gcggtcgcgc ccgtgttcct cgacctggtg
     2401 cagcatgtag acgggcgcat gcaggaagat gagcaagagg gccagcggcc aggatcccat
     2461 caggatcggc acgatggcga ggagggcaag acccatgaag agcgcgcctg caacccaacg
     2521 gctggccagc cactcccgga cggactcgat catgacattt cccccgacgc gtgccgcagc
     2581 tcaccggctg tttctgggag agtcatgaca gcttcatctt tacatcaact taagtaagca
     2641 cccgaaaagc ctaccttctg ctgctgcggg acgtgagcta aagtctcgcc aggcgctgtt
     2701 cgaagctctc gaccatcagg ccgctgcgcc cggccttcac gatggcaaag ccttcggctt
     2761 ccaggcggcg gcggtgttcc gccaacccgc ccggcatgcg aggattgagc tgtccgtcgg
     2821 tcttcagcac ccgccaccag ggcgtcaccc tctttgcgcc catcatttcc cgctcttcgg
     2881 ccgcccgcgc taccagcccc atgaagatgc cggtggtgac cggacaggca attgtggcgc
     2941 cgtgctgcgc ggcgagcgac tggcgcagag tggcaagagt tgccagcatt cccttcggaa
     3001 tccgggccac cagcgcatcc acctccgccg gcgccggaat cacca
//
LOCUS       DBNGVR010000227         1636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_476686, whole genome shotgun sequence.
ACCESSION   DBNGVR010000227 DBNGVR010000000
VERSION     DBNGVR010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_476686"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1087)
                     /gene="corA"
                     /locus_tag="AC16A0_04980"
     CDS             complement(74..1087)
                     /gene="corA"
                     /locus_tag="AC16A0_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR3104718.1"
                     /translation="MLCIYGTENGCLIERPEGAASGIPAAIWLDLVEPSEAEEAAVEA
                     ALGIDIPTREELAEIEASSRLYQEDGAAFMTANLIRRGENDQPESSPVTFIIKDNTLI
                     TIRYHHPQAFPVYVKRAMKPQTTEMTGWGVLISLLEAVVDRAADHLERVGQIVDETSR
                     KTFGTGRTLAGTAPKLRQPRRRDVSLQQLIENIGEEGDFTSKMRESLVSIGRVVAFMQ
                     AIIDQMRDTKAMKENRARIKILQRDIVSLTDHASFLNGKISFLLDAVLGLISIEQNGI
                     IKIFSVAAVVFLPPTLVASIYGMNFDVMPELHWTFGYPFALGLMVLSAILPFLYFKRK
                     GWL"
     gene            complement(1157..>1636)
                     /locus_tag="AC16A0_04985"
     CDS             complement(1157..>1636)
                     /locus_tag="AC16A0_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3104719.1"
                     /translation="CRHVGARHVVVTDINDYRLQLCNQVTDAVTVNVSKEELGDVMAR
                     LRMSEGFDIGLEMSGAPKAFDQMVDHLIMGGKFALLGIPSGPTTVDWNRVIFKALTIK
                     GIYGREMFETWYKMIAMLQSGLDVRKVITHRMKAADFAQGFELMKQGSCGKVVLDWA"
BASE COUNT          296 a          525 c          501 g          314 t
ORIGIN      
        1 gccatagcgc gccgccgttc cgatggcgcc gccgagggcc acccagagca cggtcacgcc
       61 gccgctccgt ccgtcacagc catcccttcc gcttgaaata gaggaagggc aggattgccg
      121 agagcaccat caggccgagc gcgaagggat agccaaaggt ccagtgcagt tccggcatga
      181 cgtcgaaatt catgccatag atgctcgcca ccagcgtcgg cggaaggaag acaacggcgg
      241 ccaccgagaa gatcttgatg atgccgttct gttcgatcga gatcaggccc agcaccgcgt
      301 cgagcaggaa gctgatcttt ccgttgagaa agctcgcatg gtcggtcaac gagacgatgt
      361 cgcgctgcag gatcttgatc ctggcgcggt tctccttcat cgccttggtg tcgcgcatct
      421 ggtcgatgat ggcctgcatg aaggccacga cccggccgat ggacaccagg ctctcgcgca
      481 tcttggaggt gaagtcgccc tcttcgccga tgttctcgat gagctgctgc aggctcacgt
      541 cgcgccgccg cggctgacgc agcttgggag cggttccggc aagcgtgcgc cctgttccga
      601 aggtcttgcg cgacgtctcg tccacgatct gtccgacgcg ctcgagatgg tcggcggcgc
      661 ggtcgacaac agcctcgagc aggctgatca acacgcccca gccagtcatc tcggtggtct
      721 gcggcttcat cgcccgcttg acatagaccg gaaaggcctg gggatgatgg tagcggatgg
      781 tgatgagcgt attgtccttg atgatgaagg tgacgggcga ggattccggc tgatcgttct
      841 cgccgcggcg gatcaggttg gccgtcatga aggccgcgcc gtcctcctga tagagccggc
      901 tcgatgcctc gatctccgcc agttcctcgc gggtcggaat gtcgatgccc agcgccgcct
      961 cgaccgcggc ctcctccgct tccgagggct ccacgaggtc cagccagatg gcggctggaa
     1021 tccccgacgc ggctccctca gggcgttcga tcaggcagcc attttcagtt ccatagatac
     1081 agagcatatg gcccgtttcc catttcgcgc cgctacgcgc aaggcctgcg aagaccgacc
     1141 ggcggcatat ccggactcag gcccagtcga gcacgacctt gccgcaggat ccctgcttca
     1201 tcagttcgaa tccctgcgca aagtcggcag ccttcatgcg atgggtaatg accttgcgga
     1261 cgtccagccc cgattgcagc attgcgatca tcttgtacca ggtctcgaac atctcgcggc
     1321 cgtagatgcc cttgatggtc agcgccttga agatcacccg gttccagtcg accgtggtcg
     1381 gtccggacgg gatgcccagc agggcgaact tgccgcccat gatcaggtga tcgaccattt
     1441 ggtcgaaagc cttcggtgcc cccgacatct cgaggccaat gtcgaagccc tcgctcatcc
     1501 gcaggcgcgc catcacgtcg ccaagctctt ccttcgagac attgacggtc actgcatccg
     1561 tcacctggtt gcacagctgg agccggtagt cgttgatgtc ggtgacaacc acgtgacggg
     1621 cgccgacatg gcggca
//
LOCUS       DBNGVR010000228         2312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4207925, whole genome shotgun sequence.
ACCESSION   DBNGVR010000228 DBNGVR010000000
VERSION     DBNGVR010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4207925"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..795
                     /locus_tag="AC16A0_04990"
     CDS             133..795
                     /locus_tag="AC16A0_04990"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3104720.1"
                     /translation="MALQLGDIAPDFTAETTEGRIKFHDYIDGSWVVFFSHPKDFTPV
                     CTTELGSMAKMKHEFDKRGVKLIGISIDTVEDHQRWKQDIKDVTGASVNYPLIGDNNL
                     EVAKLYGMIHPNASGNAATRTAADNATVRNVLIIGPDKKIKLIVSYPMSTGRNFDEIL
                     RVIDSLQLTAKASVATPANWKQGEDVIILASVNDEAAKEKFPAGWKTLKPYLRVVPQP
                     KI"
     gene            complement(855..>2312)
                     /gene="gatA"
                     /locus_tag="AC16A0_04995"
     CDS             complement(855..>2312)
                     /gene="gatA"
                     /locus_tag="AC16A0_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3104721.1"
                     /translation="LTNLTIAAARDGLRKKSFSALELTNAYLAAMEKARGLNAYVVET
                     PDKARAMASASDQRLARGDAGPLEGIPLGIKDLFATEGVHTQACSHVLSGFKPPYEST
                     VSANLWRDGAVMLGKLNMDEFAMGSSNETSYYGNVINPWKRRNSNAALVPGGSSGGSA
                     AAVAAHICAAATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPIA
                     RDVRDAAIMLKSMASVDPRDTTSVDMPVPDYEAAVGQPVKGLRVGIPREYRVDGMPAE
                     IDALWQKGAQWLKEQGAEVVEISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRV
                     PGDDIVDMYENSRAAGFGREVQRRILIGTYVLSAGYYDAYYVRAQKVRTLIKRDFDQA
                     WDKVDVVLTPATPSPAFAPGEITDPVQMYLNDIFTVTVNMAGLPGIAVPAGLSSEGLP
                     LGLQLVGKPFDEETLFRAASAIEQSAGHYVATPWW"
BASE COUNT          453 a          732 c          708 g          419 t
ORIGIN      
        1 gggatgacca tctccatgcc gaagttctgc gattcccgca aagtcgccct gcagcaagcg
       61 ccggacaatt cgttctttat tccgaacgct ctttgggcta tacaacaatc atcaaccgaa
      121 ggagtacccc aaatggcatt gcagctcggc gacatagctc ccgacttcac ggcggagacg
      181 accgaaggcc gcatcaagtt ccatgattac attgatggtt cgtgggtcgt gtttttctcc
      241 catccgaagg acttcacgcc cgtctgcacc accgagcttg gctcgatggc gaagatgaaa
      301 cacgagttcg acaagcgcgg cgtaaagctc atcggcattt cgatcgacac cgtggaagat
      361 caccaacgct ggaagcagga catcaaggac gtcacgggcg catcggtgaa ctatccgttg
      421 atcggcgaca acaatctcga agtcgccaag ctttacggca tgatccatcc gaatgcctcg
      481 ggcaatgccg ccacccgcac cgcggccgac aatgccaccg tccgcaacgt gctgatcatc
      541 gggccggaca agaagatcaa gctcatcgta tcctatccga tgagcaccgg ccgcaacttc
      601 gacgagatcc tgcgcgtgat cgactccctg cagctgaccg ccaaggcctc ggttgcgacg
      661 ccggcaaact ggaagcaggg cgaagacgtg atcattctcg cctcggtcaa tgacgaggcc
      721 gccaaggaaa aattccccgc cggctggaag accttgaagc cttacctcag ggttgttccg
      781 caacccaaga tctgagcgcg cttcgtctta ccctgcagaa cgtccccggc cttgccttgc
      841 agggccgggt tttcttacca ccagggtgtc gcgacgtagt gcccagccga ctgttcgatg
      901 gctgaagccg cccggaacag tgtttcctcg tcgaagggct tgccgacaag ctggaggcca
      961 agcggcaggc cctcggaaga aagccccgcc ggaacggcga tgccaggcag tcccgccatg
     1021 ttcaccgtaa ccgtgaagat gtcgttcagg tacatctgca ccgggtcggt gatctcgccg
     1081 ggtgcaaagg ctggactcgg cgtggcgggc gtgagcacca catccacctt gtcccatgcc
     1141 tggtcgaaat cgcgcttgat cagcgtgcgg accttctgtg cccgcacata ataggcgtcg
     1201 taatagccgg ccgagagcac ataggtgccg atgaggatcc gccgctgcac ctcgcgtcca
     1261 aagccagccg cgcgcgagtt ctcatacatg tcgacgatgt cgtcgcccgg cacgcgcagg
     1321 ccgtaacgga cgccatcgta tcgggcaagg ttcgacgacg cctcggcggg cgcaacgatg
     1381 tagtaggccg gcagggcata ctttgtgtgc gggagcgaga tctccaccac ttcggccccc
     1441 tgctctttca gccactgggc gcccttctgc cacagcgcgt caatctcggc gggcatgcca
     1501 tccacccgat actcgcgtgg aattccgaca cgaaggccct tcaccggctg gccgacagca
     1561 gcttcatagt caggtaccgg catgtcgacc gaggtcgtat cccgggggtc aacgctggcc
     1621 atggacttga gcatgatggc ggcatcgcgg acgtcgcggg cgatggggcc tgcctggtcg
     1681 agcgacgagg cgaaggccac gataccccag cgcgagcagc ggccataggt cggcttgatg
     1741 ccgacggtgc cggtaaaggc cgccggctgg cgaatggacc cgccggtatc cgtggcggtg
     1801 gcggccgcgc agatatgggc agcgaccgct gcggccgacc cgccggatga cccgccaggc
     1861 accagcgccg cattggagtt tcggcgcttc cacggattga tgacattgcc gtagtagctc
     1921 gtctcgttgg atgagcccat ggcgaattcg tccatgttca gcttgcccag catcaccgcc
     1981 ccatcgcgcc acaggttcgc ggagaccgtg gactcgtagg gcggtttgaa gccgctcaga
     2041 acatggctgc aggcctgggt gtggacaccc tcggtggcga agagatcctt gatgccgagg
     2101 ggaatgccct ccagtgggcc ggcatcgccc ctcgcgagcc tctggtcgga ggctgaagcc
     2161 atggcacggg ccttgtccgg cgtctcgacc acataggcgt tgaggccgcg cgccttctcc
     2221 atggcggcaa gataggcatt ggtcagttcc agggccgaga aggacttctt gcgcagtccg
     2281 tcacgagcgg ccgcgatggt caggtttgtc ag
//
LOCUS       DBNGVR010000229         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3619891, whole genome shotgun sequence.
ACCESSION   DBNGVR010000229 DBNGVR010000000
VERSION     DBNGVR010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3619891"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="AC16A0_05000"
     CDS             complement(<1..672)
                     /locus_tag="AC16A0_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3104722.1"
                     /translation="MTDFNRPLSGLVTPRFGGIATFMRLPHVPLDQAEGLDIGIFGIP
                     WDGGTTNRPGPRHAPRQMREASAMVRPMHQTMHIVPYDLANVADLGDSPVNPASVEDA
                     LKRVEGFYTALIAMGIRPLAAGGDHLCSLPALRAVGAKQPVGMIHFDAHTDLYDGYFG
                     GFKYTHGTPVRRAIEDGVLDPRRTIQIGLRGSMYDLDDFDYGAKMGVRLVRIEEAMEE
                     GPKAIM"
     gene            complement(680..>1594)
                     /locus_tag="AC16A0_05005"
     CDS             complement(680..>1594)
                     /locus_tag="AC16A0_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3104723.1"
                     /translation="GYEDSYVQNPERHPMDQLSQVIAARGWSAARIGVEMDNYWFSAA
                     ANASLRRHLTDASFFDTTGLVNWQRTVKSDHEVDFIRRAAAIVDAMHARVFELVEPGL
                     PKNVLVAEIQKQAVLGAGGHWGDYCAFVPMLPSGLDATAPHLTWDDRPFNNNEGTFFE
                     LGGCYRRYHAPLSRTVYLGKPPQKFLDAEKAVSEATEAGLGQCRPGVTCGEVADAFFG
                     TLQKNGFTKDSRAGYSIGLSYPPDWGERTLSIRRGDRTVLQPNMTLHFMPALWLDDGG
                     LELSETILITPTGVECLCKTPRKLVVKS"
BASE COUNT          261 a          490 c          573 g          270 t
ORIGIN      
        1 catgatggcc ttggggccct cttccatcgc ctcttcgatg cgcacgaggc gcacgcccat
       61 cttcgcgcca tagtcgaagt cgtcgagatc gtacatcgag ccgcgcaggc cgatctggat
      121 ggtacgcctg ggatcgagca cgccgtcctc gatggcgcgg cggacgggcg tgccatgggt
      181 gtatttgaag ccgccgaagt agccgtcgta gagatcggta tgggcatcga aatggatcat
      241 gcccaccggc tgcttcgcgc ccaccgcgcg cagcgccggc agggagcaga gatgatcgcc
      301 gcccgctgcg agcggcctga tgcccatggc gatgagcgcc gtgtagaatc cttccacgcg
      361 cttcagcgca tcctcgacgc tggccggatt gaccgggctg tcgccgaggt cggccacatt
      421 ggcgaggtca tagggcacga tgtgcatggt ctggtgcatg ggccgcacca tggcggaggc
      481 ctcgcgcatc tggcgcggcg catggcgcgg gccagggcgg ttggtggtgc cgccatccca
      541 gggaatgccg aagatgccga tgtcgagacc ttccgcctga tcgagcggca catgcggcag
      601 gcgcatgaag gtggcgatgc cgccgaagcg cggcgtgacg agtcccgaga gcgggcggtt
      661 gaagtcggtc atgatgtcct cagctcttga ccaccagctt gcgcggcgtc ttgcacaggc
      721 attcgacgcc ggtgggcgtg atcaggatgg tttcggaaag ctccagcccg ccgtcgtcaa
      781 gccacagggc cggcatgaaa tgcagcgtca tgttgggctg aagcacggtg cggtcgccgc
      841 ggcggatcga cagggtgcgc tcgccccagt cgggcggata ggagaggccg atggaatagc
      901 cggcgcgcga atccttggtg aagccgtttt tctgcagcgt gccgaagaag gcatccgcca
      961 cctcgccgca ggtgacgccg gggcggcatt gtccgaggcc cgcctcggtc gcttccgaca
     1021 cggccttctc ggcgtcgagg aacttctgcg gcggcttgcc gagatagacg gtgcgcgaca
     1081 ggggcgcgtg gtagcggcgg tagcagccgc cgagctcgaa gaaggtgcct tcgttgttgt
     1141 tgaagggccg gtcgtcccag gtgagatgcg gcgcggtcgc atcgaggccg gaaggcagca
     1201 tgggcacgaa ggcgcagtag tcgccccagt ggccgccggc gccgagcacg gcctgcttct
     1261 ggatctcggc caccagcacg ttcttgggga ggcccggctc gaccagttcg aagaccctgg
     1321 catgcatggc atcgacgatg gcggcagcgc ggcggatgaa gtcaacctcg tggtcgctct
     1381 tgacggtgcg ctgccagttg accagcccgg tcgtgtcgaa gaacgaggca tcggtgaggt
     1441 gccggcgcag cgatgcgttg gcggcggccg agaaccagta attgtccatc tcgacgccga
     1501 tgcgcgccgc actccagccg cgcgccgcga tgacctgcga aagctggtcc atcggatggc
     1561 gttccggatt ctgcacatag ctgtcctcgt agcc
//
LOCUS       DBNGVR010000230         2215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4995739, whole genome shotgun sequence.
ACCESSION   DBNGVR010000230 DBNGVR010000000
VERSION     DBNGVR010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4995739"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="AC16A0_05010"
     CDS             complement(<1..877)
                     /locus_tag="AC16A0_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104724.1"
                     /translation="MTPRVQGILLMAASMAFFALLDGMAKHVMIHLPAFVAVFFRYLL
                     ALALTLVIVWRAGGLSLLATGHPLLQALRGLMLVSSTWLNFFAISYLQLAQTAAISFT
                     IPLWVCALSVPLLGEAVGLRRWIAVLVGFLGVLVIMRPGTEDFHPAMLLSVMAAICGA
                     LYNIATRKVGGRDRAETSLFYVGFVGSLGAALPLPWHWQMPEGTQWVFLVGMGLCGGI
                     GHFMLIQAHRLAPAAALSPFVYTQIVWMIALGFLMFGDVPDLWTLIGAAVVIASGLFV
                     FARERSLGRSGEVPAP"
     gene            complement(902..1546)
                     /locus_tag="AC16A0_05015"
     CDS             complement(902..1546)
                     /locus_tag="AC16A0_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015007674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3104725.1"
                     /translation="MTLAFAAPPAVTVPIAGSDRLFPVRRIYCVGRNYAEHVKEMGND
                     PKGTPIFFQKPADALVLDGRFPYPACSKDVHHEIELVVALGPGGKVFGHAVGLDMTRR
                     DLQAAAKAGGRPWEAAKSFDFSAPIAAITPSAAAMTGGAITLDVNGTRRQSADLADMI
                     WKVDEILAELAKLFTLAAGDLVFTGTPAGVGPVQRGDRLRGDIAGLAPLDVRIV"
     gene            complement(1543..2148)
                     /gene="pncA"
                     /locus_tag="AC16A0_05020"
     CDS             complement(1543..2148)
                     /gene="pncA"
                     /locus_tag="AC16A0_05020"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HIR3104726.1"
                     /translation="MWKDTDVLVVIDVQNDFCPGGKLAVPGGDEVVPLINRLARGFRH
                     VVLTQDWHPAEHKSFATAHPAKNPFEIAMMHYGPQTLWPDHCIQDTPGAAFHKDLHIP
                     NAELIIRKGFREEIDSYSAFFENDQTTPTGLGGYLKERGFTKVVCVGLAFDFCVRFSA
                     EDARKLGFDTEVIEAACRSIDLDGSAAATRKSFAERGVVLA"
BASE COUNT          427 a          708 c          766 g          314 t
ORIGIN      
        1 cgggtgcggg aacctcgccg ctgcggccga ggctgcgctc gcgggcgaag acgaagaggc
       61 cgctggcgat gaccacggcc gcgccgatca gggtccagag gtcaggcacg tcgccgaaca
      121 tcaggaagcc cagtgcgatc atccagacga tctgggtata gacgaagggc gagagcgccg
      181 cggcgggcgc caggcgatgc gcctggatca gcatgaaatg gccgatgccg ccgcagagcc
      241 ccatgccgac gaggaagacc cattgcgtgc cttcgggcat ctgccagtgc cagggcagcg
      301 gcagggctgc gccaagactg ccgacgaagc ccacatagaa gagcgaggtc tcggcccggt
      361 cgcggccgcc gaccttgcgc gtggcgatgt tgtagagcgc cccgcagatc gccgccatga
      421 ccgagagcag catggcagga tgaaaatcct ccgtacccgg acgcatgatg acgaggacac
      481 cgagaaaacc cacaagaacg gcgatccagc ggcgcaggcc cacggcctcg cccagcaacg
      541 gcaccgacag cgcgcagacc cagagcggga tggtgaagga gatggccgcc gtctgcgcca
      601 gttgcagata gctgatggcg aagaagttga gccaggtcga ggagaccagc atgaggccgc
      661 gcagcgcttg cagcagcgga tgacccgtgg caagaagcga taggccgccc gcccgccaca
      721 cgatgaccag cgtgagggcc agtgccagca ggtagcggaa gaagacggcc acgaaggccg
      781 ggagatgaat catgacatgc ttggccatgc catcgagcag cgcgaagaag gccatggagg
      841 ccgccatcaa caggatgccc tgaacgcgcg gtgtcattcc gctttcgccg cggcagccgg
      901 ttcagacaat ccggacatcc agcggtgcaa ggccggcgat gtcgccgcgc aaccggtcgc
      961 cgcgctgtac cgggccgacg cccgcgggcg tgccggtgaa gacgagatcg ccggcggcaa
     1021 gcgtgaagag tttcgcgagt tcggccagga tctcgtccac cttccagatc atgtcggcga
     1081 gatcggccga ctgccgccgc gtgccgttga cgtcgagcgt gatggcaccg cccgtcatcg
     1141 ccgcggcaga cggggtgatt gcggcgatcg gcgcggagaa gtcgaaggat ttcgccgcct
     1201 cccacggccg tccgcccgcc ttggccgcgg cctgcaggtc gcggcgcgtc atgtcgagac
     1261 ccaccgcatg gccgaagacc ttgccgcccg ggccaagcgc cacgacgagt tcgatctcgt
     1321 gatgcacgtc cttcgaacac gccggatagg gaaagcggcc gtccagcacg agggcgtcgg
     1381 ccggcttctg gaagaagatc ggcgtgccct tcgggtcgtt ccccatctcc ttcacatgct
     1441 ccgcgtagtt gcgtcccacg cagtagatgc ggcgcaccgg gaagagacga tcgctgcccg
     1501 caatcggaac cgtgacggcg ggcggtgcgg caaaggccag agtcacgcca gcaccacgcc
     1561 gcgctcggcg aaggacttgc gcgtcgcggc ggcagacccg tcgagatcga tcgatcggca
     1621 ggcggcctcg atgacttccg tgtcgaagcc gagcttgcgg gcgtcctcgg cggagaagcg
     1681 gacgcagaag tcgaaggcaa ggccgacgca gaccaccttg gtgaagccgc gctccttcag
     1741 atagccgcca agtcccgtcg gtgtcgtctg gtcattctcg aagaaggcgg aatagctgtc
     1801 gatctcctcg cggaacccct tgcggatgat gagctcggca ttgggaatgt ggaggtcctt
     1861 gtgaaaggcg gcacccggcg tgtcctggat gcaatggtcc ggccacagcg tctgcggacc
     1921 gtaatgcatc atggcgatct cgaaggggtt cttggccgga tgcgcagtgg cgaaggactt
     1981 gtgctctgcc ggatgccaat cctgggtgag gacgacgtgc ctgaagccgc gggccaggcg
     2041 gttgatcagc ggcaccacct cgtcgccgcc cggcacggcc agcttcccgc ccgggcagaa
     2101 gtcgttctgc acgtcgatga ccacgagcac gtcggtgtct ttccacatgg ctcaattctc
     2161 ctctgcgatg gccgcggcac agcccttgaa ggccgggaag cggtcgacga tcaca
//
LOCUS       DBNGVR010000231         4071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1265964, whole genome shotgun sequence.
ACCESSION   DBNGVR010000231 DBNGVR010000000
VERSION     DBNGVR010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1265964"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC16A0_05025"
     CDS             <1..201
                     /locus_tag="AC16A0_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104727.1"
                     /translation="DDVDALWVFGSKADAAMAEKLSAGNLKRMLTDHGLATDWYDPAQ
                     AEGPILLRHAVQVKNIWIPYGE"
     gene            203..655
                     /locus_tag="AC16A0_05030"
     CDS             203..655
                     /locus_tag="AC16A0_05030"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HIR3104728.1"
                     /translation="MLRNFDNRLTADVIQALRAMGHGDDLVIADTNFPSDSVARQTTL
                     GRLLRMDNLTAAEAAEAILSVMPLDSFVDHPAERMEMVGKPKEIPPVQAEVQKAIDKA
                     EGKSWPMGSIERFAFYERAKTAYCVIVTGETRFYGCFIFKKGVIPPKA"
     gene            complement(786..2117)
                     /locus_tag="AC16A0_05035"
     CDS             complement(786..2117)
                     /locus_tag="AC16A0_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3104729.1"
                     /translation="MSAGLSRRGWVPAVAEAHVQAIARQVAQQSADENEASLLAWVEE
                     NHRIHERDCINLNPATNMMNPKAEALLASGIGSRPSLGYPGDKYEMGLEGIEKIEIMA
                     AELAAEVFGVRHAEIRVASGAMANLYVFMAAAKPGDCMIAPPPSIGGHVTHHAAGCAG
                     LYGLVTHPAPVDAANYTVDVPALRQLAQETRPKLITIGGSLNLFPHPIGAIRESADEV
                     GALVMFDAAHMSGMIAGRAWQQPLDEGAHVMTMSTYKSLGGPPAGLILTNDDAIAERL
                     DAIAYPGLTANFDAAKSAALAMTLLDWKEYGKAYGAAMAETAKALAEALAARGLPVHA
                     AARGFTASHQFALETRDFGGGQHAAKLLRQANVLSCGIGLPIADVDGDLNGLRFGTPE
                     IVRWGMTAKDMPAVAGFIADVLTGRRAAQSVAPEVAAFRSQFDHLHFVRGA"
     gene            complement(2114..3145)
                     /locus_tag="AC16A0_05040"
     CDS             complement(2114..3145)
                     /locus_tag="AC16A0_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3104730.1"
                     /translation="MAKTRGVVIGCGFFSVNHLNGWMDLKDRCEIVAVCDSDGDKARA
                     AAQRFGIAKHYADAAQMLAAETPDFADIVTTAPSHRPLVELCAAHGVAAIVQKPLALD
                     WEDATALVGAMRKKNLPLMVHENFRFQRPLLEARALVASGEIGTPVWGRFSWRTNFNV
                     YAGQPYLLETKRFILMDIGVHVLDVARAFLGEADEVFCRTQSIKPEIAGEDMATVMLR
                     HTNGAVSVNDFTYESRQTPDPFPQTLIHVEGTLGSMRVDEGYRMSVTSPRGSRVEDVS
                     PRPVAWGQPQWMLVQESVVNTQRHWLDAFQGGYAPETSGEDNLRTFALVEASYESAET
                     GRAIRPVLV"
     gene            complement(3265..3723)
                     /locus_tag="AC16A0_05045"
     CDS             complement(3265..3723)
                     /locus_tag="AC16A0_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104731.1"
                     /translation="MEPVWQALQESGFGVHVRNSVWLYPLANVLHVLAVMGFFALVAM
                     MDARLIAGSGAAAVRDTVRRLRPWALALFLLLAATGFVLLTPEAVPIAATPAFQLKAL
                     VLLLALVNVAVNTLALRRPGEVTMAARVTAVLSLILWLGVAALGRSIAYF"
     gene            complement(3742..>4071)
                     /locus_tag="AC16A0_05050"
     CDS             complement(3742..>4071)
                     /locus_tag="AC16A0_05050"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3104732.1"
                     /translation="ALLALPLLALPAVAHHGWGSYDATKPMTITAPIAEVMIGNPHGD
                     ITLMHEGEMWHVTLAPPSRMNARGATAEVVTQGAMITAHGYPRRDGTKEIRAEWIEIG
                     GTRYQLR"
BASE COUNT          691 a         1359 c         1370 g          651 t
ORIGIN      
        1 gacgatgtcg acgcgctctg ggtcttcggt tcgaaggccg atgcggcgat ggccgagaaa
       61 ctctcggccg gaaatctcaa acgcatgctc accgatcatg gcctggccac cgactggtac
      121 gacccggcgc aggcggaagg gccgattctg ctgcgccacg ccgtgcaggt gaagaacatc
      181 tggattccct acggagaatg acatgctgag gaacttcgac aatcgcctga cggctgacgt
      241 gatccaggcc ctgcgcgcca tgggccacgg cgacgatctc gtcatcgcgg ataccaattt
      301 cccgtccgac tcggtggcgc gccagaccac gctgggcagg ctgctgcgca tggacaacct
      361 gacggcggcg gaagccgccg aggcgatcct ctcggtgatg ccgctcgaca gcttcgtcga
      421 ccatccggcc gagcgcatgg agatggtggg caagcccaag gagatcccgc cggtgcaggc
      481 cgaggtgcag aaggcgatcg acaaggccga aggcaagtcc tggcccatgg gctcgatcga
      541 gcgcttcgcc ttctacgaac gcgccaagac ggcctattgc gtgatcgtca ccggcgagac
      601 ccgcttctac ggctgcttca tcttcaagaa gggcgtcatc ccgccgaagg cgtaagggca
      661 gtcaggcact ttccccctcc gccgcttcgc ggcacctccc ccgcgttccg cgagggagga
      721 aatatctgtc atccttcccc gcttgcaggg aaggtggcgc aacgcgccgg atggggttac
      781 cgcggtcaag ccccccgcac gaagtgcagg tggtcgaact gcgaccggaa ggccgcgacc
      841 tctggcgcca cggactgtgc ggcgcgccgg cccgtcagca catcggcgat gaagcccgcc
      901 accgccggca tgtccttcgc cgtcatgccc cagcgcacga tctccggggt gccgaagcgc
      961 aggccgttga gatcgccgtc gacgtctgca atgggcaggc cgatgccgca gctgagcaca
     1021 ttggcctggc gcagaagctt tgccgcatgc tggccgccgc cgaaatccct ggtctccagc
     1081 gcgaactgat gtgacgccgt gaagccgcgg gcggcggcat ggaccggaag gccgcgcgcg
     1141 gcaagagcct cggccaacgc cttcgcggtt tcggccatgg cggcaccata ggccttgccg
     1201 tattccttcc agtcgagcag cgtcatggcc agggctgccg acttcgcggc atcgaagttg
     1261 gcggtgaggc ccggataggc aatcgcatcg aggcgctcgg caatcgcgtc gtcattggtg
     1321 aggatcaatc ccgcgggcgg gccgcccagg ctcttgtagg tgctcatggt catgacatgg
     1381 gcaccttcgt ccagcggctg ctgccaggct ctgccggcga tcatgcccga catgtgcgcc
     1441 gcatcgaaca tcaccagcgc gcccacctcg tcggcgctct cgcggatcgc gccgatcgga
     1501 tgcgggaaca ggttgaggct cccgccgatg gtgatgagct tcggcctggt ctcctgtgcc
     1561 agctggcgca gggccggaac gtcgacggtg tagttggctg catcgacagg cgcaggatgg
     1621 gtgacaaggc cataaagacc ggcacagccc gcagcatgat gagtgacatg cccgccgatc
     1681 gacggcggcg gcgcgatcat gcagtcgccg ggcttcgcgg cggccatgaa gacatagaga
     1741 ttggccatgg cgcccgaggc cacgcggatc tcggcatggc gcacgccgaa gacctcggcc
     1801 gccagttccg ccgccatgat ctcgatcttc tcgatgccct cgagccccat ctcgtacttg
     1861 tcgccgggat agccgaggga aggccttgag ccgatgccgc ttgccagcag cgcttccgcc
     1921 ttcgggttca tcatgttggt ggcggggttg aggttgatgc agtcgcgttc gtggatccgg
     1981 tggttctctt ccacccaggc gagcaggctc gcctcgttct cgtctgccga ctgctgggcc
     2041 acctggcgcg cgatggcctg cacatgcgcc tccgcgaccg cgggcaccca gccacggcgc
     2101 gacaggcctg cgctcatacc agcaccgggc ggatggcgcg gcccgtctcg gccgattcat
     2161 aactcgcctc caccagggca aaggtacgca ggttgtcctc gcccgaggtt tccggcgcat
     2221 agccgccctg gaaggcatcg agccagtggc gctgcgtgtt gaccacgctc tcctgcacca
     2281 gcatccattg cggctggccc caggcgacgg ggcgcggcga gacgtcttcc acccggctgc
     2341 cgcgcggcga ggtgaccgac atgcgatagc cctcgtccac ccgcatcgag cccagcgtgc
     2401 cctcgacatg gatcagcgtc tgcgggaagg gatcgggcgt ctggcggctc tcataggtga
     2461 agtcgttcac cgagacggca ccattggtgt ggcgcaacat gacggtggcc atgtcctcgc
     2521 cggcgatctc gggcttgatg ctctgggtgc ggcagaaaac ctcgtcggcc tcgccgagga
     2581 aggcgcgcgc cacgtcgagc acatgcacgc cgatgtccat gaggatgaag cgcttggtct
     2641 ccagcagata gggctggccg gcatagacgt tgaaattggt gcgccatgag aagcggcccc
     2701 agaccggcgt gccgatctcg cccgaggcga ccagcgcgcg ggcctccagc agcggacgct
     2761 ggaagcggaa gttctcgtgc accatgagcg gcaggttctt cttgcgcatg gcgccgacca
     2821 gcgcggtggc atcttcccag tccagggcca gcggcttctg cacgatggcc gccacgccat
     2881 gcgcggcaca gagttcgacg agcggccggt gcgagggcgc tgtggtgacg atgtcggcga
     2941 agtccggcgt ctcggcggcg agcatctggg cggcgtcggc atagtgcttc gcaatcccga
     3001 agcgctgcgc ggctgctctt gccttgtcgc cgtcgctgtc gcagaccgcc acgatctcgc
     3061 agcggtcctt caggtccatc cagccattga gatgattgac cgagaagaac ccgcagccga
     3121 tcaccacgcc ccttgtcttc gccatgtgtt cccgaccccc ttgatcccgc gccaagtggt
     3181 actggatcat tcaggaaatg caacgtgctg ctgtgcgcct gcggggattc tttcgcagct
     3241 gcgagatgcc gcggccgcaa gccgtcagaa ataggcgatg gaacgcccga gcgcggcaac
     3301 gcccagccac aggatcagcg acaggaccgc ggtgacgcgc gcggccatgg tcacctcgcc
     3361 cgggcggcgc agcgccagcg tgttgaccgc gacattgacc agggccagca gcagcaccag
     3421 ggccttgagc tggaaggcgg gagtggcggc aatcggcacg gcctcggggg tcagcagaac
     3481 gaagccggtg gcggcgagga gtaggaagag cgccaaggcc cagggccgca gacggcgcac
     3541 cgtgtcgcgc acggcggccg caccggaacc ggcgatgaga cgggcatcca tcatcgccac
     3601 cagcgcgaag aagcccatca ccgccagcac gtgcagcaca ttggccagcg ggtagagcca
     3661 gaccgaattg cgcacgtgga cgccgaagcc cgactcctgc agcgcctgcc agaccggttc
     3721 catgccgtgc cctgccgccg ctcagcgcag ctggtagcgg gtgccgccga tctcgatcca
     3781 ctcggcgcga atctccttcg tgccgtcgcg ccggggatag ccatgggcgg tgatcatggc
     3841 gccctgcgtc acgacctcgg ccgtggcgcc gcgcgcattc atccgtgatg gcggcgccag
     3901 cgtgacatgc cacatctcgc cctcgtgcat cagcgtgatg tcgccatgcg gattgccgat
     3961 catgacttcg gcaatgggcg cggtgatggt catgggcttc gtcgcgtcat aggagcccca
     4021 gccgtgatgc gcgacggcgg gcagcgcaag cagcggcagg gcaagcaggg c
//
LOCUS       DBNGVR010000232         4624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2443824, whole genome shotgun sequence.
ACCESSION   DBNGVR010000232 DBNGVR010000000
VERSION     DBNGVR010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2443824"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..964
                     /locus_tag="AC16A0_05055"
     CDS             29..964
                     /locus_tag="AC16A0_05055"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF002204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase CobB"
                     /protein_id="HIR3104733.1"
                     /translation="MLAVDARHQAQSVAALVHGFSTFRPDVSVAGVLLNRVASDRHAA
                     ILHGALGHKVLGTLRHDDSLVLPSRHLGLVQAEENQDLETFIETAALAVARETVLDRI
                     LEIAGQLVGESVIREALPPLGQRIAIARDSAFAFAYPHLLHQWQRSGASLSFFSPLAN
                     EPAEPDADAIFLPGGYPELHAARLSANAAFLAGLRMSKGLIYGECGGYMVLGEALVDA
                     RGTSHAMAGLLPVVTSFANRKLHLGYRQLTPEPGAPWSSPLRGHEFHYSSIVQEGAAD
                     RLFSARDAAGRDLAPMGLRRGKVMGSYAHAICEAH"
     gene            964..2424
                     /locus_tag="AC16A0_05060"
     CDS             964..2424
                     /locus_tag="AC16A0_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR3104734.1"
                     /translation="MARAIMLMGTGSDVGKSLIAAGLCRAFARRGLRVRPFKPQNMSN
                     NAAVTAEGGEIGRAQALQARAAGIAPSVHMNPILLKPETTTGAQVIVQGQRQATMAAR
                     DYFRSRPRFMPAILDSFRKLAAEADLVIVEGAGSPGEINLRDGDLANMGFAAAANVPA
                     ILIGDIHRGGVIASIAGTFAILPPEDRNRLKAFLINKFHGDPELFSEGRAFLEAQTGT
                     PCLGILPHFAEASALPAEDAVALQEVLATNSGHCRISVLRLPRIANFDDLDPLRHEAG
                     VSVTFVNPGEVIPAESRLVIIPGSKSTMADLAALRREGWDVDLKAHARRGGLVLGLCG
                     GFQMLGRFVHDPLGLEGNAGTAEGLAMLDVETRLIPDKTLTLATGTHVPTGTAISGYE
                     IHLGITNGPDCSRPFAMIGDRPDGARSPSGQVTGTYLHGCFASDGFRAAFLQSLGAEA
                     SALRFEDRIERTLDALASHLETHLDLDRLLAMAEPV"
     gene            2424..3377
                     /gene="cbiB"
                     /locus_tag="AC16A0_05065"
     CDS             2424..3377
                     /gene="cbiB"
                     /locus_tag="AC16A0_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR3104735.1"
                     /translation="MWAFPAFAAAALALFIERLVGYPTYLLDRIGHPVIWVGKLITWL
                     DAHLNRNALSPADGRLRGVLALVVIIAAALIPAWFISHLLGFWRNGWAIEALLATSLI
                     AQKSMRDHVLAVYRGLSSSLAEGRRAVAHIVGRDPARLDESGVARAALESLAENTSDG
                     IVAPVFWFALLGLPGIAIYKAVNTADSMTGHKSERHLHFGWAAARFDDLINLPCSRLT
                     GVLFAASQGRRFGDIIDVMRRDAPKHQSPNAGWPEAAMAAALEIRLGGPRFYDGERLD
                     LAWMGEGRSDLTRIDIRRGLKVYGRAMTILLIALVLLSLVS"
     gene            complement(3343..3873)
                     /gene="cobU"
                     /locus_tag="AC16A0_05070"
     CDS             complement(3343..3873)
                     /gene="cobU"
                     /locus_tag="AC16A0_05070"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR3104736.1"
                     /translation="MRQVTLVLGGARSGKSRFAEELAERSGPLRTYIATAEAFDGEMQ
                     SRIDQHKTRRGEGWQTIEAPLALTDALAACGSPVVLIDCLTLWISNLMFRDRDVASEI
                     DLLCAALVRVSGHVILVSNEVGMGIVPENALSRRFRDMQGLANQKVAAVADAVHFVAA
                     GLPLTLKTPAIEAPAR"
     gene            complement(3870..4244)
                     /locus_tag="AC16A0_05075"
     CDS             complement(3870..4244)
                     /locus_tag="AC16A0_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 family protein"
                     /protein_id="HIR3104737.1"
                     /translation="MRAIVMCTTCRYSAGEKTGPDGRTGGETLIAHMSEALQARGMAS
                     VALQTQACLWNCKRHCSVILQDDQRFTYFTGDHEPSRAQADAILDWFQAHGESGTGEV
                     PFRLWPDRMRGHFIARIPPVKP"
     gene            4324..>4624
                     /locus_tag="AC16A0_05080"
     CDS             4324..>4624
                     /locus_tag="AC16A0_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR3104738.1"
                     /translation="MKFSRIAFLASDSPEARKAVGRLTRRHGNADPDSADVVVALGGD
                     GHMLQILHRFVSTGTPIYGMNRGTIGFLMNDFQDNHLPERLQAAEMTTIHPLRMVA"
BASE COUNT          789 a         1567 c         1396 g          872 t
ORIGIN      
        1 cctggcatcc agcctgggac tgcccgtcat cctcgccgtc gatgcccggc accaggcgca
       61 atccgtcgcg gccctggtac acggcttctc cacctttcgc ccggacgtct cggtggcagg
      121 ggtattgctc aaccgcgtcg ccagcgaccg ccatgccgcg attctgcatg gcgcgctggg
      181 acacaaggtt ctcggcacgc tgcggcatga cgactcactc gttctcccat cgcggcacct
      241 cgggctggtg caagcagaag agaatcaaga tcttgaaact tttattgaaa ctgccgcctt
      301 agccgttgca cgtgaaacag ttcttgacag gattctcgag attgcaggcc aactcgttgg
      361 cgaatcagtg attcgggaag cgctgccccc tttggggcaa cggatcgcca ttgcccgcga
      421 ctcggccttc gcctttgcct acccgcatct tctgcaccag tggcagcggt cgggggcaag
      481 cctcagcttc ttttcgcctc tggcaaacga gccagccgaa cccgacgcgg atgccatctt
      541 ccttcccgga ggctatccag agctccatgc cgcacggctc tcggcaaatg ccgcattcct
      601 cgccggcctc cgcatgtcca agggcctcat ctatggcgag tgcggaggat acatggttct
      661 cggcgaagcg ctggtcgatg ccaggggcac gtcccatgcc atggctggcc ttctgccggt
      721 ggtgacgagt ttcgccaatc gtaagctcca tctcggctat cgccagctga cccccgaacc
      781 aggagcgccg tggtcgtcgc cgctgcgcgg tcacgaattc cactactcgt ccatagtcca
      841 ggaaggcgct gccgaccgtc tcttctcggc aagagatgca gccggacggg atcttgctcc
      901 catggggctg cgccgcggca aggtcatggg ctcctacgca cacgccatct gcgaggccca
      961 ttgatggcca gggcgatcat gctcatgggc acggggtcgg acgtcggcaa gtcgctgatc
     1021 gcggcaggtc tctgccgggc ctttgcccgg cgcggactga gggtccgccc cttcaagccg
     1081 cagaacatgt cgaacaatgc cgccgtcacg gccgaaggcg gagaaatcgg ccgggctcag
     1141 gcccttcagg cgcgcgcggc aggcatcgcc ccgtcggttc acatgaatcc catcctgctg
     1201 aagcccgaaa ccacgaccgg cgcgcaggtc atcgtgcagg gacagcggca ggccacgatg
     1261 gccgcacgcg attacttccg cagccgcccc cggttcatgc ccgccattct cgacagtttc
     1321 aggaagttgg ctgctgaagc cgaccttgtt atcgttgagg gtgctggcag cccgggcgag
     1381 atcaacctcc gcgacggcga cctcgccaac atgggatttg cggcggcggc caatgtgccg
     1441 gcaatcctca tcggggacat tcatcgcggc ggagtcattg cctcgatcgc cggcaccttc
     1501 gcaatcctgc ctcccgagga tcgaaacaga ctaaaggctt tcttgatcaa caagttccat
     1561 ggcgaccctg aactgttctc tgagggccgc gcctttctgg aggcgcaaac cggaacgcct
     1621 tgcctcggca tcctgccgca ttttgcggag gccagcgctt tgccggcgga agatgcagtg
     1681 gccttgcagg aagtcctcgc aaccaactca ggtcattgca ggatttcggt tcttcgcctg
     1741 ccgcgcatcg ccaatttcga tgatctcgac ccgttgcgtc acgaagcggg tgtctccgtc
     1801 accttcgtca atccgggcga ggtcatcccc gccgagagcc ggctggtcat tatcccgggc
     1861 tcgaaatcca caatggccga tctcgcggcg ttgcgccgcg agggctggga tgtcgacctg
     1921 aaggctcacg ccaggcgcgg cggtctcgtg ctgggcctct gcggcggctt ccagatgctg
     1981 ggtcgcttcg tccacgaccc cctcggactt gagggcaatg ccggtaccgc agaaggcctg
     2041 gccatgcttg atgtcgagac ccgcctcatc cctgacaaga cccttaccct agcgacgggc
     2101 actcatgtgc cgaccggaac ggcgatttca ggttacgaga tccacctggg catcaccaat
     2161 gggccagact gctcccgccc ctttgcgatg atcggcgacc ggcctgacgg agcgcgatcg
     2221 ccgtctggcc aggtgaccgg gacctatctc catggctgct ttgccagtga tggcttcagg
     2281 gcggccttcc tgcaaagcct cggcgccgaa gcgtcagcgc tgcgcttcga ggaccgcatc
     2341 gagcggaccc tcgacgctct ggcctcccat ctcgagaccc acctcgacct cgacagactg
     2401 ctggccatgg cggaaccggt ctgatgtggg ccttcccagc cttcgctgcg gctgcccttg
     2461 ctctcttcat cgagcgactg gtcggctacc ctacatatct tctcgatcgt atcggccacc
     2521 ctgtgatctg ggtcggcaag ctcattacat ggctggacgc ccatctaaac cgaaacgccc
     2581 tttcgcctgc cgacgggcgg cttcggggcg tgctggcgct cgtcgtcatc atcgccgctg
     2641 cattgattcc tgcctggttc atctcccatc tgctggggtt ttggcgcaat ggctgggcca
     2701 tcgaagccct tctggccacc agcttgattg cccagaagtc gatgcgggat cacgtgcttg
     2761 cggtctaccg cggcctgtcc tcgtcactgg ccgaagggcg ccgggccgtg gcgcatatcg
     2821 tcggtcgcga cccggcccgt ctagacgaga gcggggtggc ccgcgccgca ctcgaaagcc
     2881 tggccgagaa cacgtcagat ggcatcgttg cgccggtctt ctggtttgcg cttctgggcc
     2941 ttcccggcat cgccatctac aaggccgtga acacagcgga ttcgatgacc ggccacaagt
     3001 cggaacggca tctgcatttc ggctgggcgg ccgcgcgctt cgacgacctg atcaacctcc
     3061 cctgctcccg cctcaccggg gtcctgttcg ccgcctccca gggcagacgg tttggcgaca
     3121 tcatcgacgt catgcggcgc gatgcaccaa agcaccaatc gcccaatgcc ggatggcccg
     3181 aggccgccat ggcggctgca cttgagatca ggctgggcgg tccgcgcttc tatgacggtg
     3241 agcggctcga ccttgcctgg atgggggaag gccgcagcga tcttacccgt atcgacatcc
     3301 ggcgcggcct caaggtctat ggccgcgcga tgaccattct gctcatcgcg ctggtgcttc
     3361 tatcgctggt gtcttgaggg tcaacggcaa gccggcagcc acgaagtgaa cggcgtccgc
     3421 cacggccgcc accttctgat tggccagccc ttgcatgtcc cgaaagcggc gcgacagggc
     3481 gttctccgga acgatgccca tgcccacttc gttcgagacg agaatgacgt ggcccgaaac
     3541 cctcaccaag gccgcgcaga ggaggtcgat ctcggacgca acatcgcggt cgcggaacat
     3601 cagattgctg atccacagcg tcaggcagtc gatgaggacg acaggacttc cgcaggcagc
     3661 cagcgcatcg gtcagagcca gaggcgcctc gatggtctgc cagccctccc cgcgccgcgt
     3721 cttgtgctgg tcgatccgag actgcatttc gccgtcgaag gcttccgcag tggcgatgta
     3781 ggtgcggagc ggaccagacc tttcggccag ttcttccgca aagcggctct tgcccgatcg
     3841 cgcgccgccc agcaccaaag tgacctgcct cacggcttca ccggcggaat gcgggcgatg
     3901 aaatgccccc gcatgcggtc aggccaaagc cggaacggta cctcgcccgt cccgctctcg
     3961 ccatgggctt ggaaccagtc caggatggca tcggcctggg ccctgctcgg ttcgtggtct
     4021 ccggtaaagt aggtgaagcg ctgatcatcc tgcaggatca cgctgcaatg ccgcttgcag
     4081 ttccacaggc aggcctgcgt ctgcagcgct acagaggcca tgccgcgggc ctgaagcgcc
     4141 tccgacatgt gcgcaatcaa tgtctcgccg ccggtccgcc cgtcgggtcc ggttttctcg
     4201 cccgccgaat agcggcaggt ggtacacatg acgatggcac gcatcgggtt ccgttcaggg
     4261 attgtcaaga ccggtgcctt caatgtaggc aaagacaggg cctgtcaacg cgaagaaacc
     4321 ggcatgaaat tctcccgtat cgccttcctg gcgtccgact cgcccgaagc ccgcaaagcc
     4381 gtcggccgcc tcaccaggcg ccatggcaac gccgatccgg acagcgccga tgtcgtggtg
     4441 gccctcggcg gtgacgggca catgctgcag atcctgcacc gcttcgtgtc gaccggcacg
     4501 ccgatctacg gcatgaaccg cggaacgatc ggcttcctga tgaacgactt tcaggacaac
     4561 catctcccgg agcggctgca ggccgccgag atgaccacca tccatcccct ccgaatggtg
     4621 gcac
//
LOCUS       DBNGVR010000233         2784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3230306, whole genome shotgun sequence.
ACCESSION   DBNGVR010000233 DBNGVR010000000
VERSION     DBNGVR010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3230306"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="AC16A0_05085"
     CDS             complement(<1..668)
                     /locus_tag="AC16A0_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3104739.1"
                     /translation="MLKYLRNTGLAAISLAAMAIAAEAKTTVTIGELDWPGARAIEQV
                     LKQVMETHLDADVATIAAAQEALYEALDKGDNSVDIVADMWTDHLPQQMKNYVLPGGR
                     ETIILNATPYLGTEGIFVPTYVVEEHGVKKLEDLAKPEVAKLFDSDGNGKGEMWAGAV
                     GWESTNHTEVRGKTYGFDKTMEYTTVEQAVFLAQLKDAIDNKKPIAFYYWTPEWIHAA
                     YKLT"
     gene            complement(715..1668)
                     /locus_tag="AC16A0_05090"
     CDS             complement(715..1668)
                     /locus_tag="AC16A0_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020812990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104740.1"
                     /translation="MRKADPGKLSAHFSWLMALVLALAAAWWLLAPALPEWFTSFPAP
                     PGLDNAAALAIDNAVTWMKQKWVGFFDVFTLILRTILNVIEKAMVATPWPLTAAAAIL
                     TAWRLSGPRAAVFTSACLAYLGLFGFWEKSMSTLALVAASVLICVAIGLPLGILCAKS
                     PRTKRLLEPVLDVMQTLPTFVYLIPAVAFFSIGKPPGVIATVIFALPSMVRLTALGIE
                     QVPAVVREAADAFGARPLQKLMKVELPLALPSIRLGINQTIMMSLSMVVVAAMIGAGG
                     LGLDVIRSLQHLKTGQGVLAGLAIVLCAMALDRMVRGKPPG"
     gene            complement(1655..2707)
                     /locus_tag="AC16A0_05095"
     CDS             complement(1655..2707)
                     /locus_tag="AC16A0_05095"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR3104741.1"
                     /translation="MAEPAIELRHVWKVFGSIAEPEFERLRRDGGSKGDIQERLGCVV
                     AVADASFSIAPGEIFCIMGLSGSGKSTLLRHINGLVAPTAGEVLVGGINIAALSAATL
                     RDLRARKIGMVFQNFALLPHRSVLDNVAFGLELRDVPQPERLDRARAMLKAVQLEAWA
                     DAGITELSGGMQQRVGLARALAGDPDILLMDEPFGALDPLIRRQLQDQFVGLCRTMNK
                     TAVFITHDLDEAMRIGSRIAIMRDGRILQIGAPHEIILAPADAHVAAFVHDVSSRDTL
                     SARHVMSKLRPGERKTSAKAQGVEADTVLAEAARLAAREKRDLPVRDSSGQIIGMLRI
                     PDMLSALAGRGQHAQG"
BASE COUNT          499 a          894 c          904 g          487 t
ORIGIN      
        1 ttggtcagct tgtaggccgc atggatccat tcgggagtcc agtagtagaa ggcgatgggc
       61 ttcttgttgt cgatggcatc cttgagctgg gccaggaaaa cggcctgctc cacagtggtg
      121 tattccatcg tcttgtcgaa gccataggtc ttgccgcgca cttccgtgtg gttggtcgat
      181 tcccagccca ccgcaccggc ccacatttcg cccttgccgt tgccgtcgct gtcgaacagc
      241 ttggccactt cgggcttcgc caggtcttcc agcttcttca ccccatgctc ctcgaccaca
      301 taggtgggca cgaagatgcc ctcggttccg agataggggg tggcattgag gatgatggtt
      361 tcgcggccgc ccgggagcac gtagttcttc atctgctggg gcagatggtc ggtccacatg
      421 tcggccacga tgtcgaccga gttgtcgccc ttgtcgagcg cttcgtagag ggcctcctgt
      481 gcagcggcaa ttgtggccac atccgcatcg agatgggtct ccatcacctg cttcagcacc
      541 tgctcgatcg cccgtgcgcc cggccagtca agttcgccga tggtcaccgt ggtcttggcc
      601 tcggcggcaa tcgccatcgc cgcgagagaa atggccgcga ggccggtgtt cctgagatat
      661 ttcaacatgt cttcctcctt gtgtggttgt cgtccttatg tctccgccgg tgtttcatcc
      721 cggcggcttt ccgcgcacca tgcggtcaag cgccatggcg cacaaaacga tggcaaggcc
      781 ggcgagaacg ccctgtccgg tcttgagatg ctgcagcgaa cggatgacat cgaggccgag
      841 gccgccggca ccgatcatgg cggcgacgac gaccatcgac agcgacatca tgatggtctg
      901 gttgatgccg agcctgatcg acggcaaggc gaggggaagc tcgaccttca tcagcttctg
      961 caagggccgt gccccgaagg catctgctgc ctcgcgcacc acggccggaa cttgttcgat
     1021 ccccagtgcc gtcagtcgca ccatcgaggg caaggcaaag atcacggttg cgatgacgcc
     1081 cggcggcttg ccgatcgaga agaaggccac ggccggaatc aggtagacga aggtcggcag
     1141 cgtctgcatc acgtcgagca ccggttcgag aagccgcttg gtgcgcggac tcttggcgca
     1201 gagaatgccg agcggcaggc cgatcgcaac gcagatcaga accgaggcgg cgaccagcgc
     1261 cagcgtcgac atgctcttct cccagaagcc gaagaggccg agataggcga ggcaggccga
     1321 ggtgaaaact gcagcacgag ggccggagag acgccaggcg gtcaggatcg ccgccgccgc
     1381 cgtcagcggc cagggggttg cgaccatggc cttttcgatg acgttcagga tggtgcggag
     1441 gatcagcgtg aagacatcga agaagccgac ccacttctgc ttcatccagg tgacggcatt
     1501 gtcgatggca agggccgcgg cattgtcgag acccggggga gcgggaaagc ttgtgaacca
     1561 ttcaggcagg gccggcgcga gcagccacca ggcggcggca agggcgagca cgagggccat
     1621 gagccaactg aaatgggccg acagctttcc gggatcagcc ttgcgcatgc tgacctcgtc
     1681 ccgccagtgc cgaaagcatg tccggaatgc gcagcatgcc gatgatctgt cccgaagaat
     1741 cgcgcaccgg aagatcgcgc ttctcgcgcg ccgcgaggcg tgccgcttcg gccagcacgg
     1801 tgtccgcttc gacgccttgc gccttggcgg aggttttccg ctcgcccggc ctgagcttcg
     1861 acatcacgtg cctcgcactc agcgtgtcgc gcgaggagac atcgtgaacg aaggcggcga
     1921 catgtgcatc cgccggcgcg agaatgatct catgcggtgc gccgatctgc aggatccgcc
     1981 cgtcgcgcat gatggcgatg cggctgccga tgcgcatcgc ctcgtcgaga tcgtgggtga
     2041 tgaacacggc cgtcttgttc atggtccggc acaggccgac gaactggtcc tgcagctgcc
     2101 ggcggatcag cgggtcgagt gcaccgaagg gttcgtccat cagcaggatg tcgggatcgc
     2161 cggccagcgc ccgcgccagg ccgacgcgct gttgcatgcc acccgaaagc tccgtgatgc
     2221 ccgcatccgc ccaggcctcg agctgaacgg ccttcagcat cgcccgcgcc cggtccagcc
     2281 tctcgggctg cggcacgtcg cgcagctcca ggccaaaggc gacattgtcg agcacgctcc
     2341 tgtgcggcag aagcgcaaaa ttctggaaga ccatgccgat cttccgggcg cgcaggtcac
     2401 gcagcgtagc ggcggagagc gccgcgatat tgatgccgcc gaccagcacc tctcccgcgg
     2461 tgggcgccac cagaccgttg atgtggcgca gcagggtgga cttgccgctg ccggacaggc
     2521 ccatgatgca gaagatctcg cccggcgcga tgctgaaact cgcatcggca accgccacaa
     2581 cgcagcccag gcgctcctgg atgtcgccct tgctgccgcc gtcgcgccgc agtcgctcga
     2641 actcgggctc cgcgatcgag ccaaagacct tccagacgtg gcgcaactcg atcgcgggct
     2701 ctgccatcac cacaaccctc cgagcgcgac ggtgacggct tcgccattga tcccggcggc
     2761 cgcgtcgctg gcgaggaacg agat
//
LOCUS       DBNGVR010000234         3507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4604667, whole genome shotgun sequence.
ACCESSION   DBNGVR010000234 DBNGVR010000000
VERSION     DBNGVR010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4604667"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..904)
                     /locus_tag="AC16A0_05100"
     CDS             complement(86..904)
                     /locus_tag="AC16A0_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3104742.1"
                     /translation="MLGKGVRWFDDWFAIEQVAPGVTAIGEPRFHQLNWNYLIEGNRR
                     ALLLDTGPGVRDIAKVVRALTNLPVTALPSHMHFDHTGGLAAFDNIAIADLPVLRNCV
                     ADGWLRPTDELYVGYWEGMTWTPVKVSQWLEIGREIDLGGRQLALLHTPGHSPDSVSL
                     LDAKADIFFAADFVYPGKLYAQIEGADLKAYLDSATALLPRLTDTTAILCAHGKPDAE
                     RLHAAPRLARQDVADLAASLTRLRDSGERPDRWPVNDRLSLLLWEPAFATWQSP"
     gene            complement(904..1833)
                     /locus_tag="AC16A0_05105"
     CDS             complement(904..1833)
                     /locus_tag="AC16A0_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HIR3104743.1"
                     /translation="MHPAYSVILFTTASGAGYGLLALLGLVGFNHGQASSVAFALVSV
                     FVALALITVGLLSSTFHLGHPERAWRALSQWRSSWLSREGLAALATYVPALLFAAVWS
                     GLVDAPNWIGPLGLITATLCALTVFTTGMIYRSLRTIRAWHQPLTVPVYLVFALATGA
                     TLITAIATLFGRFQPFQVILGVAALLLAMLLKFLYWRAVDAMPPAYTMNDATGLGRIG
                     DVSQWEVPHTSENYIQKEMGYAVARKHAAKLRKLVFLLLAAAILLMLLALLAPVVSLL
                     AVIAALSAAVVERWLFFAEARHVVTLFYGGRPV"
     gene            complement(1835..2632)
                     /locus_tag="AC16A0_05110"
     CDS             complement(1835..2632)
                     /locus_tag="AC16A0_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3104744.1"
                     /translation="MTSLPPPAAKKLGLVIDLDTCVGCQACVTSCKEWNTQGYSAPLS
                     DYDPYGSDPDGVWLNRVHGYEVKEQGQDQTRIVHFPRSCLHCETPACVTVCPTGASYK
                     RAEDGIVLVNPDTCIGCKLCSWACPYGAREYDLAQGVMKKCTLCVDRIYNENLEPEDR
                     QPACVKACPTGARHFGDLGDPVSPVSQLVAERGGYDLLPEMGYRPVNKYLPPRARRDA
                     TNTSEIALPKPDGSAFDRLFAWADRLLSAKAGASKADLTDYSGKLEG"
     gene            complement(2629..>3507)
                     /locus_tag="AC16A0_05115"
     CDS             complement(2629..>3507)
                     /locus_tag="AC16A0_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR3104745.1"
                     /translation="ERAPGLGPLAGFRGSNGKAQGRGAPNPEQLQHYIDNGCHWFYEL
                     PDHMKYYRHVNWAYLDWATFFGFVGKPDPIIHQLYCEPLQKFRLAAAGHGKVVPPAAH
                     RARIRDHFDPIPFWYPPFEGQMVSEADYPMHAVTQRPMAMYHSWGAQNAWLRQILGRN
                     WLYMARQRAGELGIADGDWVTVTSHHGTIKAQAKLMDGVNPDTAWTWNAIGKRSGAWG
                     LSPDAPEAKKGFLLNHLISELLPEREGGYRYSNSDPVTGQAAWYDLRVRIEKAAGQEP
                     ESAPQFATLKKLKRAK"
BASE COUNT          648 a         1093 c         1209 g          557 t
ORIGIN      
        1 cggctggcca gcgcttcggt gaaggacgga cgggacatgc agacctcttg ggaaaaaaga
       61 atataaagaa gtctttatat gattttcagg ggctttgcca agtggcaaac gccggttccc
      121 agagcagcag cgacaggcgg tcgttgacag gccagcgatc cggcctctcc ccgctgtctc
      181 tcaggcgggt caggctggcg gcaaggtcgg ccacatcctg ccgggccagg cgcggcgcag
      241 catgaagccg ctcggcatcg ggcttgccgt gggcgcacag gatggcggtg gtgtccgtca
      301 ggcgcggcag aagcgccgtg gccgagtcga gataggcctt caggtcggcg ccctcgatct
      361 gggcatagag cttgccggga tagacgaagt cggcggcgaa gaagatgtcc gccttggcgt
      421 cgagcagcga aaccgagtcg ggcgaatggc ccggcgtatg gaggagtgcg agctgccgtc
      481 cgccgaggtc gatctcccgg ccgatttcaa gccactggct gacctttacc ggcgtccagg
      541 tcatgccctc ccaataaccc acatagagtt cgtcggtcgg gcgcagccat ccatccgcga
      601 cgcagttgcg cagcaccggt aggtcggcga tggcgatgtt gtcgaaggcg gcaaggcctc
      661 cggtatggtc gaaatgcatg tgcgagggca gtgccgtcac cggcagattg gtcagggcac
      721 ggacgacctt cgcgatgtcg cgcacgcccg gtcccgtgtc gagcaggagg gcgcggcggt
      781 ttccttcgat caggtagttc cagttcagct ggtgaaagcg cggctcgccg atggccgtca
      841 cgcccggtgc gacctgctcg atggcgaacc aatcgtcgaa ccagcgcacg cccttgccca
      901 gcatcagacc ggcctcccgc catagaacaa cgtcacgaca tgccgggcct cggcgaagaa
      961 cagccagcgc tcgaccaccg ccgccgaaag tgccgcgatg acggccagca gcgagacgac
     1021 cggcgccagg agcgccagga gcatcaggag gatcgcagcc gcgagcaaga ggaagacgag
     1081 cttccgcaat ttggccgcgt gcttgcgggc gaccgcatag cccatttcct tctggatgta
     1141 gttctccgag gtgtgcggca cctcccattg cgagacgtct ccgatgcggc cgaggccggt
     1201 ggcgtcgttc atcgtatagg ctggcggcat ggcatcgacg gcgcgccagt aaaggaactt
     1261 gagcagcatc gccagcagca gggcggcgac gccgagaatg acctggaagg gctggaaccg
     1321 cccgaagagc gtggcgatcg ccgtgatcag cgtcgcgcct gtcgccaggg cgaatacgag
     1381 atagaccggc acggtgagcg gctgatgcca ggcgcggatg gtgcgcagcg agcgatagat
     1441 catgccggtg gtgaaaaccg tcagggcaca gagcgtcgcg gtgatgaggc cgagcgggcc
     1501 gatccagttc ggcgcatcga cgaggcctga ccagacggca gcaaagagca gtgcgggaac
     1561 ataggtggca agcgcggcaa gcccctcgcg cgacagccag ctggaccgcc actgcgacag
     1621 cgcgcgccag gcgcgttccg gatggccgag gtggaaggtg gacgacagca gtcccaccgt
     1681 gatcagcgcc agcgcgacga agaccgagac cagcgcaaag gcgacgctcg aggcctggcc
     1741 atggttgaat cccacgagcc cgagcaaggc cagcagccca taacctgcgc cggacgcggt
     1801 cgtgaagagg atgaccgaat aggcgggatg catctcagcc ctcgagcttg ccggaatagt
     1861 cggtcaggtc ggctttggat gcgccagcct tggccgagag cagccggtcg gcccaggcga
     1921 agagccggtc gaaggcagat ccgtcaggct tcggcagcgc gatctccgac gtgttcgtcg
     1981 catcgcggcg ggcgcgcggc ggcaggtact tgttgaccgg ccgatagccc atctcgggca
     2041 gcaggtcata gccgccccgc tccgcaacga gctgcgacac gggagaaacc ggatcgccga
     2101 gatcgccgaa atggcgggcc ccggtcgggc aggccttcac gcaggcgggc tggcggtcct
     2161 ccggctccag gttttcgttg tagatgcggt ccacacagag cgtgcacttc ttcatcacgc
     2221 cctgggccag gtcatactca cgcgcgccat agggacaggc ccaggaacag agcttgcagc
     2281 cgatgcaggt gtccgggttc accagcacga tgccgtcttc cgcgcgcttg taggaggcgc
     2341 cggtggggca gacggtcacg caggccggcg tttcgcaatg caggcaagag cgcgggaaat
     2401 gcacgatgcg ggtctggtcc tggccctgct ccttcacctc gtagccgtgc acccggttga
     2461 gccagacgcc gtccgggtcg gatccatagg ggtcgtagtc gctgagcggc gccgaatagc
     2521 cttgcgtgtt ccactccttg caggaggtga cgcaggcctg gcagccgacg caggtgtcga
     2581 gatcgatcac caggcccagt ttctttgcgg cgggcggcgg cagcgaggtc atttcgccct
     2641 cttcagcttc ttcagagtcg cgaactgcgg cgcgctctcc ggctcctggc cggcggcctt
     2701 ctcgatgcgg acacggaggt cgtaccaggc cgcctgcccg gtcaccggat cgctgttgga
     2761 atagcggtag ccgccttcac gttccggcag aagttccgag atcaagtgat tcagcagaaa
     2821 gccctttttc gcctccggcg catccggaga aagaccccag gcgccggacc gcttgccgat
     2881 cgcattccac gtccaggccg tgtccggatt gacaccatcc atgagcttcg cctgcgcctt
     2941 gatcgtcccg tgatgcgagg tgacggtcac ccagtcgcca tcggcgatgc cgagctcgcc
     3001 cgcccgctgg cgcgccatgt agagccagtt gcgcccgagg atctggcgca gccaggcatt
     3061 ctgcgcgccc caggagtgat acatggccat ggggcgctgc gtcaccgcat gcatgggata
     3121 gtcggcctcg ctcaccatct ggccctcgaa gggcggatac cagaagggaa tgggatcgaa
     3181 gtgatcgcgg atgcgggcgc gatgcgcggc gggcggaaca accttgccgt ggcctgccgc
     3241 ggccagacgg aatttctgca acggctcgca gtaaagctgg tggatgatcg gatcgggctt
     3301 gccgacgaag ccaaagaagg tggcccagtc gagataggcc cagttcacat ggcggtagta
     3361 cttcatgtga tcgggcagct cgtagaacca gtggcagcca ttgtcgatgt aatgctgcaa
     3421 ctgttcggga ttgggcgccc cccgcccctg cgccttgccg ttggaaccgc ggaagccggc
     3481 gagcggaccg agccccggcg cgcgttc
//
LOCUS       DBNGVR010000235         1773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_875344, whole genome shotgun sequence.
ACCESSION   DBNGVR010000235 DBNGVR010000000
VERSION     DBNGVR010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_875344"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..>1773
                     /locus_tag="AC16A0_05120"
     CDS             432..>1773
                     /locus_tag="AC16A0_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HIR3104746.1"
                     /translation="MNMHMLPEGLPEAHGLYDPRNEHDACGIGLIANIHNAKSHRVVA
                     DGLSILKNLEHRGAVGADPKAGDGAGVMIQIPHGFFTAEAKRLGFELPGPQHYGVGQL
                     FMPREPVYRQDIERIWWETSREEGLKVLGWRDVPVDSTVLGYSVKGTEPVHRQVFIGR
                     GPTIRDEAHFERKLFVCRKVVSNRVLEVLGSKAKTYYPVSVSARTIVYKGLVLGKSLG
                     DYYLDLVDPRVESAFAMVHQRFSTNTFPSWPLAHPYRFVCHNGEINTVRGNYNWMAAR
                     QANMQSEILGEDLSKLWPISYEGQSDTACFDNALEFLTMGGYSLPHAMMMLIPEAWAG
                     NPLMDEDRRAFYEYHAALMEPWDGPAAMVFTDGRLIGATLDRNGLRPSRYMTSADGHV
                     LLASEMGCLQFAEENITHKWRLQPGKMLLIDLEQKRIISDEELKKQLAAAHPYKD"
BASE COUNT          354 a          602 c          521 g          296 t
ORIGIN      
        1 tccgaacagc ctcttcctga tccatggtcg cctcacgaaa tattattgct gatttttcgg
       61 tctgccgatt ccttccttgc gcggcgcccc tcaatctgcg ataaagcaat aggacagtat
      121 cactgtcctt tcgtggtgaa tttcaaggcg agtggacccg gatgttccgg aatacgacca
      181 caaagaacct gaaagcggcg gccaaaccgg ctgtcgacac cgcgcgcatg ccccgggcga
      241 cacttcgtaa aacgtcgtaa gggcaggccc ggacaccgac gaaggtgccg ctggtctgac
      301 ctccgaaccg ggcacgggac ccccagggtc tccccctcac gagacaggca cggcacgaca
      361 cgatccttca caggcttcca cccggaggca tgtgagacaa ggctggggaa cggacgaaga
      421 aggagttggc catgaacatg cacatgctgc cagaggggct tcccgaagcg cacggtctct
      481 acgacccgcg caacgagcac gacgcctgcg gcatcggcct catcgccaat atccacaatg
      541 ccaagagcca tcgcgtggtg gccgacggac tctccattct caagaacctc gagcatcgcg
      601 gcgccgtggg tgccgacccc aaggccggcg acggcgccgg cgtcatgatc cagatcccgc
      661 acggcttctt cacggcggaa gccaagcgcc tcggcttcga actgcccggg ccgcagcatt
      721 acggcgtggg acaactcttc atgccgcgcg agccggtcta ccgccaggac attgagcgca
      781 tctggtggga aacgagccgc gaagagggcc tcaaggttct gggctggcgc gatgtgccgg
      841 tcgattcgac cgtactcggc tactcggtga agggcaccga gcccgtgcat cgccaggtct
      901 tcatcggccg cggacccacc atccgcgacg aggcgcattt cgagcgcaag ctcttcgtct
      961 gccgcaaggt ggtctcgaac cgcgtgctgg aagtgctggg cagcaaggcg aagacctatt
     1021 atccggtctc ggtctcggcc cgcaccatcg tctacaaggg cctcgtgctg ggcaagagcc
     1081 tcggcgacta ctacctggac ctcgtcgacc cgcgcgtcga gtcggccttc gccatggtgc
     1141 accagcgctt ctcgaccaac actttcccga gctggccgct ggcacatccc taccgcttcg
     1201 tctgccacaa cggcgaaatc aacacggtgc gcggcaacta caactggatg gcggcgcggc
     1261 aggccaacat gcagtccgag attctgggcg aagacctctc gaagctctgg cccatctcct
     1321 atgagggcca atccgacacc gcctgcttcg acaatgcgct cgagttcctc accatgggcg
     1381 gctacagcct gccccatgcc atgatgatgc tgatcccgga agcctgggcc ggcaacccgc
     1441 tgatggacga ggaccgccgc gccttctacg agtatcacgc ggccctcatg gagccctggg
     1501 acggcccggc cgccatggtc ttcaccgacg gacgcctgat cggcgcgacg ctggaccgca
     1561 acggcctgcg cccctcgcgc tacatgacct cggccgacgg tcatgtgctg ctcgcctccg
     1621 agatgggctg cctgcaattc gcggaagaaa acatcaccca caagtggcgc ctgcagcccg
     1681 gcaagatgct gctgatcgat ctcgaacaga agcgcatcat ctcggacgag gagctgaaga
     1741 agcagcttgc ggccgcacat ccctacaagg act
//
LOCUS       DBNGVR010000236         1619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_86933, whole genome shotgun sequence.
ACCESSION   DBNGVR010000236 DBNGVR010000000
VERSION     DBNGVR010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_86933"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16A0_05125"
     CDS             complement(<1..347)
                     /locus_tag="AC16A0_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HIR3104747.1"
                     /translation="MSRLSVFSSPLLLGFDELERMLDRASKTSGDGYPPYNIERLPRA
                     EGEAERLRITLAVAGFGRDELDVTVEENQLVIRGRQKEEDGRAYLHRGIAARQFTRSF
                     VLADGMEVTGARL"
     gene            552..1553
                     /locus_tag="AC16A0_05130"
     CDS             552..1553
                     /locus_tag="AC16A0_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR3104748.1"
                     /translation="MLRRTLLGAVTALAVSAASAFSAENKPAIVFDIGGKFDKSFNES
                     MFNGAEKFKAESGIAYGEFEIANEAQREQAIRNFADQGYSPIIAAGFAQAAAVEKVAK
                     EYPELKFAIVDMVVDLPNVQSIVFKEHEGSYLVGLLAGMASKSGKVGFVGGMDIPLIR
                     KFACGYAQGVKAAKPDATIFQNMTGDTGAAWNDPVKGGEITKGQMAQGADVIYAAAGA
                     TGLGVLQAAADGGALSIGVDANQNYLHPGKVLTSMLKRVDVAVYNVMKAGKDGTFKSG
                     ILALGLKEDGVGYAMDEHNKDLVTAEMQAAAEKAKADIIAGTIKVHDYTADNTCPVQ"
BASE COUNT          323 a          513 c          502 g          281 t
ORIGIN      
        1 tccagccggg ctccggtcac ttccatgccg tccgccagca cgaagctccg cgtgaactgg
       61 cgggccgcga tgccgcggtg caggtaagcc cttccgtctt cctccttctg gcggccgcgg
      121 atcaccagct ggttctcctc gaccgtcacg tccagctcgt cgcggccgaa acctgcgacg
      181 gcgagcgtga tgcgcagccg ttccgcctcg ccctcggcac gcggcagccg ttcgatattg
      241 tagggtggat agccgtctcc ggacgtcttg gaagcccggt cgagcatccg ctccagctcg
      301 tcgaagccga gcaggagggg cgatgaaaaa accgacagac gcgacatgtt tgccctcgtg
      361 aagcagcaat cgaagctgga acccggaagc ggcatcctca gctacaacca tcatatgtga
      421 aagctgttcc ccggattcaa gccgggagct tgaccttacg gcttgcccgg tgttttgatc
      481 atggacaaca aagggggtcg cagcctatgc cgggttgtgg ttagatcgcg caatcaacag
      541 ggagaccatt catgctcaga cgtacacttc tcggcgccgt gaccgcgctc gcggtatcgg
      601 cggcttcggc cttttccgcg gagaacaagc ccgccatcgt gttcgacatc ggcggcaagt
      661 tcgacaagtc ctttaacgaa agcatgttca acggtgccga gaagttcaag gcggagagcg
      721 gcatcgccta tggcgagttc gagatcgcca acgaggcgca gcgcgagcag gcgatccgca
      781 acttcgccga ccagggctat tcgccgatca tcgcggcagg cttcgcgcag gctgccgccg
      841 tcgagaaggt cgccaaggaa tatccggaac tcaagttcgc catcgtcgat atggtggtgg
      901 acctgccgaa cgtccagtcc atcgtcttca aggagcatga gggctcctat ctcgtcggcc
      961 tcctcgccgg catggcctcc aagtccggca aggtgggctt cgtgggcggc atggacattc
     1021 cgctgatccg caaattcgcc tgcggctatg cccagggcgt gaaggccgca aagcccgacg
     1081 ccaccatctt ccagaacatg acgggcgaca ccggcgccgc ctggaacgat cccgtcaagg
     1141 gcggcgagat caccaagggc cagatggccc agggtgcgga cgtgatttac gcggcagcgg
     1201 gtgcgactgg cctcggtgtg ctgcaggccg cggccgatgg cggagcactt tcgatcggcg
     1261 tcgacgccaa ccagaactac ctccatcctg gcaaggtgct gacctccatg ctgaagcgcg
     1321 tggacgttgc cgtctataac gtgatgaagg ctggcaagga cggcaccttc aagagtggta
     1381 tcctcgcctt gggcctcaag gaagatggcg tcggctatgc catggatgaa cacaacaagg
     1441 atctggtgac tgcggaaatg caggctgccg ccgaaaaggc caaggccgac atcatcgccg
     1501 gcaccatcaa ggtgcacgac tacaccgccg acaacacctg cccggtgcag taagaaacgc
     1561 tcctgatccc cttccccccc cccctcatgg gggaaggtgg cgcgaagcgc cggatgagg
//
LOCUS       DBNGVR010000237         3978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5390360, whole genome shotgun sequence.
ACCESSION   DBNGVR010000237 DBNGVR010000000
VERSION     DBNGVR010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5390360"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..906)
                     /locus_tag="AC16A0_05135"
     CDS             complement(28..906)
                     /locus_tag="AC16A0_05135"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3104749.1"
                     /translation="MSFELALRQAAQATDALLDQLLPLEASPARRIMEAVRYAALGPG
                     KRLRPFLLVEAASLFDVPRQQSLRAGAALECIHCYSLVHDDLPAMDDDDLRRGRPTTH
                     KAFDEATAILAGDALLTFAFEILSDAETHPDATIQLALVSGLAKAAGQAGMVGGQMLD
                     IEAETQANSNLSDIVRIQSLKTGALFRFACEAGAVMGASDPAPLRRYADSIGLAFQIA
                     DDILDVESTPEALGKATQKDKGMGKATFVDFLGLDGARRKASGLVDEAVAALAPYGAK
                     ADLLRDAARFIVARRK"
     gene            995..1696
                     /gene="mtgA"
                     /locus_tag="AC16A0_05140"
     CDS             995..1696
                     /gene="mtgA"
                     /locus_tag="AC16A0_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR3104750.1"
                     /translation="MAGTAARGWRRYLRIGGYVLAALVAWPIVMTVVYTVVPPPFSNV
                     MLLRALSGNGIKKDWVGLDEISPHLPRAVITAEDARFCEHRGVDWTEFQGVIEDALEA
                     GEAPLRGASTIPMQTAKNLFLWDGRFVIRKVLELPVALWMDLVWSKQRMIEIYLNIVE
                     WAPGVYGAEAAARHHFKKSARNLTRREAALLAAVLPNPIKRSAGKPSKGVRSIANRIQ
                     VRMAGMGPYLACLGR"
     gene            1767..1952
                     /gene="rpmF"
                     /locus_tag="AC16A0_05145"
     CDS             1767..1952
                     /gene="rpmF"
                     /locus_tag="AC16A0_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3104751.1"
                     /translation="MAVPRKKMSKSRIGMRRSGHQLGTVTWVEDKKSGELRRPHHIDL
                     KSGVYKGRQVLTVKTDA"
     gene            2037..3134
                     /locus_tag="AC16A0_05150"
     CDS             2037..3134
                     /locus_tag="AC16A0_05150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3104752.1"
                     /translation="MLVPNWTLMECRLVPGGLGAEAATRHLATRGLSACGPNDPELVL
                     ASLRHLGGDLALVQDSDGLRLSMPVVRRSLPLPLLGNRASPISTFGPPHLDADLAVPA
                     LTAFLRHVGAPVLIQSVPRDGAAWEALTAAGQVAEVERWHRAGLQVSGSFDAWFERNF
                     ERKRRKEYRRLKARLAEAGHLVARSLAPGEDPLPWISRFLDVEAASWKGGRGTALKSD
                     AKATAALRDACTALARHGRLRFWILELDGRPIAALYATVDRGQAWLGKIAFDPAFAKF
                     SPGVLVVLHATERIFAEGGIALVDSCAIPGHPMIDNIWRDRIAMADVVVAHPGFSRAA
                     FAATAMAERARRLGHAALRGLRNRMTGRHRS"
     gene            3131..>3978
                     /locus_tag="AC16A0_05155"
     CDS             3131..>3978
                     /locus_tag="AC16A0_05155"
                     /inference="COORDINATES: protein motif:HMM:NF025007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HIR3104753.1"
                     /translation="MSLVEIDPATARSKFLKQAFTLKHTLADHPLFTLPRLVHLAKSV
                     PRDRIEYNSGKVPVGVKPEDVPKIDRSADEVIRAIETANAWMVIKNVEEDTDYRALLR
                     TFVEDANRAAGRGAGDYTDLQGFIFVSSAQATTPFHIDAEENILIQIRGDKFVRTFDN
                     DDRKLISEEDMEISPSKHRNQKYENWYEQRATLHSLKPGDALHMPYMIPHWVSTGDTY
                     SISMAMTWKTPEVLRLNKIRLMNGTLRRFGLPQKPPGVSPALDMMKVIAHDVMRAVID
                     PIRKSET"
BASE COUNT          678 a         1322 c         1312 g          666 t
ORIGIN      
        1 gtccggcagc gctggggctg acgcccgcta cttgcgccgc gccacgatga agcgggcggc
       61 gtccctcagc aggtcggcct tggcgccata gggcgcgagg gccgcgacgg cttcatccac
      121 aaggccgctt gccttgcgcc gcgcgccatc cagccccaga aagtcgacga aggtcgcctt
      181 gcccatgccc ttgtccttct gggtcgcctt gccgagcgcc tccggggtcg actccacgtc
      241 gaggatgtcg tcggcgatct ggaaggcgag gccgatggag tcggcatagc gccggagggg
      301 cgcaggatcg cttgcaccca tcaccgcgcc cgcctcgcag gcaaagcgga agagggcgcc
      361 cgtcttcaga ctctgaatcc ggacaatgtc cgacagattt gaattcgctt gtgtttctgc
      421 ttcgatatcg agcatctgcc cgcccaccat gccggcctgg ccggcggcct tcgcaaggcc
      481 cgagaccagg gccaactgga tcgtggcgtc cggatgggtc tcggcatcgg agaggatctc
      541 gaaggcaaag gtcagcagtg cgtcgcctgc gaggatcgcc gtggcctcgt caaaggcctt
      601 gtgcgtcgtc ggccgcccgc gccgcaggtc gtcgtcgtcc atggccggga gatcgtcatg
      661 gacgagcgaa tagcagtgga tgcattccag cgccgcgccc gcccgcagcg attgctgacg
      721 cgggacatcg aacaggctgg ccgcctcgac cagcaggaac ggccgcagcc gcttgcccgg
      781 gcccagggcc gcgtagcgca cggcctccat gatgcgccgg gccggtgagg cttcgagcgg
      841 cagcagctgg tcgagcaggg cgtcggttgc ctgggcggcc tggcggaggg caagttcgaa
      901 ggacaaggca atcttcctgt gctatgcggc ccttagtagg gacatgagcg acgaaactca
      961 accagcagcc ttgccgcgcg agggagacag gtcgatggca ggcacggcag cacggggctg
     1021 gcggcggtac ctgcgcatcg gcggctatgt gttggcagcc ctggttgcct ggcccatcgt
     1081 gatgacggtg gtctacacgg tcgtgccgcc gcccttttcc aacgtcatgc tgctgcgggc
     1141 cctgtccggc aacgggatca agaaggactg ggtcggcctg gacgagattt ccccccatct
     1201 gccgcgcgcc gtgatcaccg cggaggatgc ccgcttctgc gaacaccgcg gcgtcgactg
     1261 gaccgagttc cagggcgtga tcgaggatgc actggaagcc ggcgaagcgc cgctccgggg
     1321 ggccagcacc attcccatgc aaacggccaa gaacctgttc ctgtgggacg ggcgctttgt
     1381 catccgcaag gtgctggagc tgccggtggc gctgtggatg gatctggtct ggtccaagca
     1441 gcgcatgatc gagatctatc tcaacatcgt cgaatgggcg ccgggcgtct atggcgcgga
     1501 agcggcggcc cgccatcatt tcaagaaatc cgccaggaac ctcacccgga gggaggcggc
     1561 gctgctggcg gctgtcctgc ccaaccccat caagcgcagc gctggaaagc cctccaaggg
     1621 ggtccggtcc atcgccaaca ggatccaggt gcggatggcg ggaatgggcc cctatctcgc
     1681 ttgccttggg cgctgagcgc aggtataagc cgccccaact tgagtggcgc ggcagttccc
     1741 gcgccacaat cacatggagt tctacaatgg ccgttccacg caagaaaatg tccaagagcc
     1801 gcatcggcat gcgccggtcc ggccaccagc tcggcaccgt gacctgggtc gaggacaaga
     1861 agtcgggtga gcttcgccgc ccgcaccaca tcgacctgaa gtccggcgtt tacaagggcc
     1921 gtcaggttct gaccgtcaag accgacgcct gatctggcgc ttaacctgtt gatttgaaaa
     1981 gccggccgcg aggccggctt tttcgttgca ggcacgggcc ttgcacgggc tttcccatgc
     2041 ttgtgccaaa ctggacattg atggagtgcc gcctcgttcc cggaggtttg ggcgccgaag
     2101 ccgcgacccg gcacctcgcc acccgcggcc tgtcggcctg cggtccgaac gacccggaac
     2161 tggttctggc cagcctgcgg cacctggggg gtgaccttgc ccttgtccag gacagcgatg
     2221 gcctccgcct gtccatgccg gtggtccgcc gcagcctgcc gctgccgctg ctcggcaacc
     2281 gggcctcgcc catctctact ttcggtccgc cgcatctgga cgccgacctc gccgtcccgg
     2341 cgctcacggc ctttctccgc cacgtggggg cacccgtcct gatccagtcc gttccgcgcg
     2401 atggcgccgc gtgggaggcc ctgacggcgg caggccaggt cgccgaggtc gagcgctggc
     2461 accgcgcagg ccttcaggtc tccggcagct tcgatgcgtg gttcgagcgg aacttcgagc
     2521 gcaagcgccg caaggaatac cgccggctca aggcgcgcct cgccgaggcg gggcatctcg
     2581 tggcccgcag cctggcgcct ggcgaagacc ccttgccctg gatctctcgc ttcctcgatg
     2641 tcgaagcggc cagctggaag ggcgggcgcg gcacggcgct caagtccgac gcaaaggcca
     2701 cggctgcgct gagggacgcc tgcacggcgc ttgccaggca cggacggctc cgcttctgga
     2761 tccttgaact cgatggccgg cccatcgccg cgctctacgc caccgtcgat cgcggtcagg
     2821 cctggcttgg caagatcgcc ttcgatcccg cctttgcgaa gttttcgccc ggcgtactgg
     2881 tcgtgctgca cgccaccgaa cggatctttg ccgaaggcgg aatcgcgctg gtcgattcct
     2941 gcgccattcc cggccacccg atgatcgaca acatctggcg cgatcgcatc gccatggcgg
     3001 acgtggtggt ggcgcatccg ggtttcagtc gcgccgcctt tgccgccacc gcgatggccg
     3061 agcgggccag gcgcctcggc catgccgcgc tgcgcggcct tcgcaacaga atgacaggga
     3121 gacaccgttc atgagcctcg tcgagatcga tcccgccacc gcgcggagca agttcctgaa
     3181 acaggccttc acgctgaagc acacgctggc ggatcatccg ctcttcacgc tgccgcgcct
     3241 ggtgcatctg gcgaagtccg ttccgcgcga ccgcatcgaa tacaactctg gcaaggtgcc
     3301 ggtgggggtg aagccggagg atgttccgaa gatcgaccgc agcgccgatg aggtgatccg
     3361 cgcgatcgag accgccaatg cctggatggt gatcaagaac gtcgaggagg acacggacta
     3421 ccgggcgctc ttgaggacct tcgtggaaga tgccaatcgc gccgcgggcc ggggggccgg
     3481 cgactacacc gatctgcagg gtttcatctt cgtgtcctcg gcccaggcca ccacgccctt
     3541 tcatatcgac gccgaggaga acatcctgat ccagatccgc ggcgacaagt tcgtgcgcac
     3601 gttcgacaat gacgaccgca agctgatttc cgaggaagac atggagatct cgccgtcgaa
     3661 gcaccggaac cagaagtatg aaaactggta tgagcaacgc gcgacgttgc acagcctgaa
     3721 gcccggcgat gcactgcaca tgccctacat gatcccgcat tgggtgagca cgggcgatac
     3781 ctattcgatc tcgatggcga tgacctggaa gacgccggaa gtcctgcgcc taaacaagat
     3841 ccgcctgatg aacggcacgc tgcggcgctt cggcctgccg cagaagccgc ccggcgtttc
     3901 gccagcgctc gacatgatga aggtgatcgc ccacgacgtg atgcgcgcgg tgatcgaccc
     3961 gatccggaaa tcagagac
//
LOCUS       DBNGVR010000238         3630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3230603, whole genome shotgun sequence.
ACCESSION   DBNGVR010000238 DBNGVR010000000
VERSION     DBNGVR010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3230603"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC16A0_05160"
     CDS             complement(<1..395)
                     /locus_tag="AC16A0_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HIR3104754.1"
                     /translation="MTEPVLNASPKTWDEVKTTTCYMCACRCGIKVYLKDGTVRYIDG
                     NRDHPVNKGVICGKGASGIMQHYSPARLSKPLIRVGERGSGEFREIEWEEALRTATLW
                     LSEVRNSDPKKLAFFTGRDQSQGLTGWWST"
     gene            480..1952
                     /gene="pdxR"
                     /locus_tag="AC16A0_05165"
     CDS             480..1952
                     /gene="pdxR"
                     /locus_tag="AC16A0_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR3104755.1"
                     /translation="MRDWLVHIRRSEHASLQTQIREALVSAILDGQLAREEPIPSTRK
                     MAKSLSVSRNTVVLAYQGMLDDGYLVARERSGYYVAEKALDSVTPQRLPPAARSAGEI
                     DWATRLVKDPAAQENITKPLDWQSYAYPFIYGQVDHNLFPLAEWRDCARQALGKKWLG
                     SWTNDTWAHDDPLLVEQIRRRILPRRGIMASDDEILITLGAQNALYLLTSLLAGPDTR
                     VAMEEPGYPDMRNIFRLKTRNVALIPVDEQGITFTDPLRDANIVFTTPSHQFPTTVTM
                     PLERRMELLKLASQRDMVVIEDDYEFETNYVNEPCPALKSLDDEGRVIYVGSLSKTLF
                     PGLRLGFMVGPKTLIAEARALRRLMVRHAPNNNQRTAALFLSLGHHDTLIRRLHREYR
                     SRWEVMGAALNAHMPNASRIPSFGGTSFWVTGPQGLDSEDLARAAAARGILIEPGRIN
                     FGVAKPPRNHFRLAFSSIDEAKIEPGVRLLADLVQAAGRA"
     gene            2022..2966
                     /locus_tag="AC16A0_05170"
     CDS             2022..2966
                     /locus_tag="AC16A0_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104756.1"
                     /translation="MAAATHNDNRLLAIACILAGVGLASTQDAIVKSISGGYPVYEAL
                     MLRCIGSIPILGIFVWREKALGELLTPPALGLIFIRGLILGFAYLCFVLAIAAIPIAN
                     AVAIYFTMPFFVAGLAGPLLGERVRLHRWLAIIAGFIGVLIMVRPGAGVFEPASIFAL
                     LSALGYAVGQMIGRPLSQQVSPVVIAVWQNLIYFGIGLALALAFNFAFKPDFEHPSLV
                     FLTRALVMPNLPDLLLMLFSGVLAAFGMMLFVNAYKYGEANFVAPFEYSAMIWAVFFG
                     LVLFNDFPDPLTWTGAAIVIAAGLLMVWRDRQLNRAGG"
     gene            3072..3533
                     /locus_tag="AC16A0_05175"
     CDS             3072..3533
                     /locus_tag="AC16A0_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104757.1"
                     /translation="MESDIKRKFGLVWPAHVGNLTRLLIAARQAFDGDLDMFLVLAII
                     GDRTFSARNADPALTFEAWQTGSAPHVATEDINIRSIADFSGIPRETVRRKLAQLIDK
                     GWVVRGDNNALAATLKARNELAPLTEESLRYLIQMFHLFEGIAGEPPRVTG"
BASE COUNT          594 a         1283 c         1099 g          654 t
ORIGIN      
        1 gtggaccacc agccggtgag cccctggctc tgatcgcgcc cggtgaagaa ggcgagcttc
       61 ttcggatcgc tgttgcgcac ctccgagagc cacagggtgg cggtgcgcag cgcctcctcc
      121 cactcgatct cgcggaattc gcccgagccc cgctcgccca cccggatcag cggcttggac
      181 agccgcgccg gggaataatg ctgcatgatg ccggaagcgc ccttgccgca gatcacgccc
      241 ttgttcaccg ggtgatctcg gttgccatcg atgtatctga cggtgccgtc cttgagatag
      301 accttgatgc cgcagcggca ggcgcacatg tagcatgtgg tcgtcttgac ctcgtcccag
      361 gtcttgggcg aggcgttgag aacgggttcg gtcatcggct tccgctggcg tttccgggct
      421 accttatccc gcgaattggc actatgatag gtccagacgc cttattcaca gggaccaatg
      481 tgcgcgactg gcttgtccat atccggcgca gcgagcatgc cagcctgcag acccagatcc
      541 gcgaggccct ggtctcggcc atcctcgacg gacagctggc gcgcgaggaa ccgatcccct
      601 ccacccgaaa gatggccaag agcctgtcgg tgtcgcgcaa caccgtggtg ctggcctatc
      661 agggcatgct ggacgatggc tatctcgtgg cccgcgagcg ctcgggctat tatgtcgccg
      721 agaaggcgct ggacagcgtc acgccgcagc gccttccgcc cgcagcgcgg tctgccggcg
      781 agatcgactg ggccacccgc ctcgtcaagg atccggccgc gcaggagaac atcaccaagc
      841 cgctggactg gcagagctac gcctatccct tcatctacgg ccaggtggac cacaatctct
      901 ttcccctggc cgagtggcgc gactgcgcgc ggcaggcgct gggcaagaaa tggctgggct
      961 cctggaccaa cgacacctgg gcccatgacg atccgctgct ggtcgagcag atccgccgcc
     1021 gcatcctgcc gcgccgcggc atcatggcca gcgacgacga gatcctcatc acgctgggcg
     1081 cgcagaatgc gctctacctg ctgaccagcc tgctggccgg tcccgacacc cgcgtggcga
     1141 tggaagagcc gggctatccc gacatgcgca acatcttccg gctgaagacg cgcaacgtgg
     1201 cgctgatccc cgtcgatgaa cagggcatca ccttcaccga tccgctgcgc gacgccaaca
     1261 ttgtcttcac cacgcccagc caccagttcc cgaccaccgt caccatgccg ctggagcggc
     1321 gcatggaact tttgaaactc gcctcgcagc gcgacatggt ggtgatcgag gacgactacg
     1381 aattcgagac caactacgtc aacgaaccct gcccggcgct caagtcactc gatgacgagg
     1441 gccgcgtcat ctacgtgggg tcgctctcca agacgctgtt ccccggcctg cgcctcggct
     1501 tcatggtggg accgaagacg ctgatcgccg aggcccgcgc gctccgccgc ctgatggtgc
     1561 gccacgcgcc caacaacaac cagcgcaccg cggcgctgtt cctgtcgctc ggccatcacg
     1621 acacgctgat ccgccgcctg caccgcgaat accgcagccg ctgggaggtg atgggtgccg
     1681 cgctcaacgc gcatatgccc aatgcctcgc gcattccgag cttcggcggc accagtttct
     1741 gggtgaccgg accccagggc ctggacagcg aagacctggc cagggccgcg gctgccagag
     1801 gcatcctcat cgagcccggg cgcatcaact tcggcgtggc caagccgccg cgcaatcact
     1861 tccgcctcgc cttctcgtcc atcgacgagg caaagatcga gccgggcgtg cgcctgctcg
     1921 cggacctcgt ccaggccgca ggccgcgcct gaggccacgc atggatgacc tgcgatggcg
     1981 ccccttccgg ccatcgcgcc cgcgggctaa gtgcagccgt catggccgcc gccacccata
     2041 acgacaaccg ccttctcgcg atcgcctgca tccttgcggg cgtcggcctt gcttcgaccc
     2101 aggacgccat cgtcaagtcg atctccggcg gctatcccgt ctacgaggcg ctgatgctgc
     2161 gctgcatcgg gtccatcccg atcctcggaa tcttcgtctg gcgcgagaag gcgctgggcg
     2221 agctgctgac gccgccggcg ctcggtctca tcttcattcg cggcctgatc ctcggattcg
     2281 cctatctctg cttcgtgctc gccatcgcgg cgatccccat cgccaacgcg gtggcgatct
     2341 acttcaccat gcccttcttc gtcgccggac tggcgggccc gctgctcggc gaacgcgtgc
     2401 ggctgcaccg ctggctggcc atcatcgcgg gattcatcgg cgtcctgatc atggtccggc
     2461 ccggtgcggg cgtcttcgaa ccggcatcga tcttcgccct gctctcggcg ctgggctacg
     2521 cggtggggca gatgatcggg cgcccgctgt cgcagcaggt gagccccgtc gtcattgccg
     2581 tctggcagaa cctcatctac ttcggcatcg gacttgcact ggcgctggcc ttcaacttcg
     2641 ccttcaaacc cgacttcgag catcccagcc tcgtctttct cacccgcgca ctggtgatgc
     2701 ccaacctgcc cgacctcctg ctcatgctct tctccggtgt gctcgccgcc ttcggcatga
     2761 tgctcttcgt caacgcctac aagtatggcg aggccaattt cgtggcgccc ttcgaatact
     2821 cggcgatgat ctgggccgtc ttcttcggcc tggtcctgtt caacgacttc cccgatccgc
     2881 tgacctggac cggcgccgcc atcgtgatcg ccgccggcct gctcatggtc tggcgcgacc
     2941 gccagctcaa tcgcgcgggc gggtgagcgc tgtcaccgcg agcgggcctg gcacttcttg
     3001 gcgcggctcc gctgggataa ggacaatccg attgaaccgt cgggacaagg ggacggcagg
     3061 gagcgttgaa cgtggaatct gacatcaaac ggaaattcgg cctggtctgg cccgcgcatg
     3121 tcggcaacct gacgcgattg ctgattgccg cacggcaggc cttcgacggc gacctcgaca
     3181 tgttcctggt cctggccatc atcggtgacc ggacattctc ggcccgcaat gcggatcccg
     3241 cgctgacctt cgaagcctgg cagacgggca gcgcaccgca cgttgcgacg gaagacatca
     3301 acatccgctc gatcgcagat ttcagcggca ttccccgtga gaccgttcgc cgcaagctcg
     3361 cccaattgat cgacaaggga tgggtcgtgc gcggggacaa caacgcgctg gccgccacgc
     3421 tgaaggcgcg caacgagctt gcgcccctga ccgaggaaag ccttcggtac ctcattcaga
     3481 tgttccatct gttcgagggc attgccggcg aaccgccgcg tgtaaccggg taacgcgccc
     3541 tatcgagccg cacggcgctg ctgctcgacc caaattgggc cacacgccgg tttaccggag
     3601 cgccggggct tgccaagttc aaccgctagc
//
LOCUS       DBNGVR010000239         3174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3622846, whole genome shotgun sequence.
ACCESSION   DBNGVR010000239 DBNGVR010000000
VERSION     DBNGVR010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3622846"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="AC16A0_05180"
     CDS             complement(<1..728)
                     /locus_tag="AC16A0_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104758.1"
                     /translation="MSKSPIPRPTGLAMLIAGSAVDSTSGLFSRLITADGFTTASGRG
                     FFAFLLLTLVLIWRDRNRTLRSLLGIGLGGIAFVLLNASGMVMNMLSLKFTAVANFFM
                     IFATAPFVAALAGRIVLGEKLDLATLLAALAGFAGIAIMMVSGASSGGLIGDLLALAC
                     VLTYAAIVMVVRRNPQLDMLPVLALTVLTSGLIALPFASFGGLPGHDWAILVLFGFFQ
                     LAVGNLLIFAAVSRIPAAQSGLLG"
     gene            836..2143
                     /locus_tag="AC16A0_05185"
     CDS             836..2143
                     /locus_tag="AC16A0_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3104759.1"
                     /translation="MGYIEIIVVVLLTALNGLLAMSELAVASSRMPKLRAMAEQGVHG
                     ARRAMRLASDPGRFLSTVQIGITLIGILAGAVSGAALGQRVSLWFASQGLSETVSEAL
                     GYGLVISAITYLSLIIGELVPKQIALRNPERIACMVAPAMTLLARVAAPIVWFLDRSG
                     RLVLALLGQTRQRDETVTDAEIHSMIAEAESAGVIEPEEREMIAGVMRLGDRPVRSVM
                     VPRADVQMIDMNDTPARIAKVLADSGHSRFVVYKDDPDNIVGVLQAKDVAVSLLRKKV
                     LNVKQLVKQAPVIPDTVDALDVVGRLKESEVHFGLVYDEYGHFEGIVTTADILEAIAG
                     AFREERQAEEPEMTERSDGSLLIAGWTPIDHAMQRLGLPAPERRDYQTMAGFVLDHLG
                     HLPVLGEAVEHRGYRFEVVDLDGRRIDMVMVQKITPQTRRPVI"
     gene            2140..2766
                     /locus_tag="AC16A0_05190"
     CDS             2140..2766
                     /locus_tag="AC16A0_05190"
                     /inference="COORDINATES: protein motif:HMM:NF017833.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HIR3104760.1"
                     /translation="MNDEPKKPKPRRLSEIVSSFEGREGFTIGAMVDDFGERAFGALM
                     FIFAVPNVIPAPPGTSAILGLPLVILTFQLMLGRQALWLPDRIRRRPVNSDMVNSFVR
                     KAVPILKRFETILRPRLSLIVMSNAAERVIGVVTLLLAVVLFLPIPLVNILPAAAIAC
                     LALALAERDGLFAIIGYLLALSTVFILFYVSTALFAAAKAFVGTLFGL"
     gene            2991..>3174
                     /locus_tag="AC16A0_05195"
     CDS             2991..>3174
                     /locus_tag="AC16A0_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3104761.1"
                     /translation="MQTGTVKWFNAQKGFGFIEPSSGGPDVFVHISAVERAGMTGLNE
                     GQKISYEIVTDRRNGKS"
BASE COUNT          599 a          957 c         1018 g          600 t
ORIGIN      
        1 atgccaagta ggccggactg tgcggcaggg atacggctga cggctgcgaa gatcaggaga
       61 ttgccgaccg caagctggaa gaaaccgaac aggacaagta tcgcccagtc atggccgggc
      121 agaccgccaa agcttgcgaa cggcagggca atcaggccgc tcgtcagcac tgtcagcgcg
      181 aggaccggca acatgtcgag ctggggattg cggcgcacca ccatcacgat cgcggcgtag
      241 gtcagcacgc aggccaatgc caggagatcg ccgatgaggc cgcccgagct ggcaccggaa
      301 accatcatga tggcgatccc ggcaaagcct gcgagggcgg cgagcagggt tgcaaggtcc
      361 agtttctccc caagcacgat ccggccagcc agagccgcca cgaagggggc tgtggcaaag
      421 atcatgaaga aattggccac ggccgtgaac ttcagggaga gcatgttcat caccatgccg
      481 ctggcattca ggagcacgaa ggcgatgccg cccagcccga ttccaaggag gctgcgtagc
      541 gtgcggttcc ggtctcgcca gatcagaacg agcgtaagca agagaaatgc aaagaacccg
      601 cgtccggaag ccgtggtaaa gccgtcggcc gtgatgagcc ggctgaacag cccggaggtg
      661 gagtcgacgg ccgatccggc aatcagcatg gcgagccctg tgggccgggg aatcggggat
      721 tttgacatag gcgccttgtt cacgctttga ccggcgcttg caaccacgcg aaaacttctt
      781 cgcgccccgg ctcgaaggct gctagaaagc tccccccgat cactattccg gaccaatggg
      841 ttacatcgag atcatcgtcg tcgtcctgtt gactgccctg aatggcctgc tggccatgtc
      901 ggaactggca gttgcctcgt cccgcatgcc aaagctgagg gccatggcgg agcagggagt
      961 ccatggcgcc cgccgcgcca tgaggctggc ctcggacccg ggacggtttc tctcgaccgt
     1021 gcagattggc ataaccctga tcggcatcct cgccggcgcc gtctccggcg cggcgctggg
     1081 ccagcgggta tcgctttggt ttgccagcca gggcctgagc gagacggtct ccgaagccct
     1141 gggctatggg ctggtgatca gcgccataac ctatttgtcg ctgatcatcg gcgaactcgt
     1201 gccgaagcag atcgcgctgc gaaatcccga acgcatcgcc tgcatggtgg ccccggccat
     1261 gaccctgctg gcccgggtgg ctgcaccgat cgtgtggttt ctcgaccggt ccgggcggct
     1321 ggtgctggcc ctgctgggac agacgcgcca gcgcgacgag acggtcacgg atgctgaaat
     1381 ccactcgatg atcgccgagg cggagtcggc cggcgtcatc gaacccgaag aacgcgaaat
     1441 gatcgccggc gtgatgcgcc tgggggatcg cccggtcagg tcggtcatgg tgccgcgagc
     1501 cgacgtgcaa atgatcgaca tgaacgacac cccggcgcgg attgccaagg tattggctga
     1561 cagtggccat tcgcgctttg tggtctacaa ggacgatccc gacaacatcg tcggtgtgct
     1621 gcaggccaag gacgtcgccg tttcactgct tcgcaagaag gtgctcaacg tgaagcagct
     1681 ggtcaagcag gcgccggtca ttccggatac ggtggatgcc ctggacgtcg tgggccggct
     1741 gaaggagtcg gaagtgcatt tcggcctggt ctatgacgag tatgggcact tcgagggcat
     1801 cgtgaccacc gccgacatcc tcgaggccat tgccggcgcc ttccgcgagg agcgtcaggc
     1861 ggaggagccg gagatgaccg aacgcagcga tggctcgctg cttatcgcgg gatggacgcc
     1921 gatcgatcat gccatgcagc ggcttggcct gcctgctccc gaacgccggg actatcagac
     1981 catggctggt ttcgttctcg atcacctggg ccacctgccg gtgctcggag aagccgttga
     2041 acatcggggc taccgcttcg aggtggtcga tctggacgga cgcaggatcg acatggtgat
     2101 ggtgcagaag atcacgccgc agacccggag accggtgatt tgaacgacga gccgaaaaag
     2161 ccaaagccgc gtcgcctgtc cgagatcgtg tcgtcgttcg aggggcgtga gggcttcact
     2221 attggcgcga tggtcgacga ctttggggaa agggccttcg gcgccctgat gttcatcttt
     2281 gccgttccaa acgtcatccc ggcgcctccg ggcacttctg ccatcctcgg cttgccgctg
     2341 gtcatcctga ccttccagct gatgctgggc cgccaagccc tatggctgcc cgaccggatc
     2401 cgtcgccgcc cggtgaactc cgacatggtc aacagcttcg tgcgcaaggc ggtgccaatc
     2461 ctcaagcgct tcgagaccat cctccggccc agactatccc tgattgtcat gtcgaatgcc
     2521 gccgagcggg tcatcggcgt ggtgaccttg ctgctggcgg tggttctctt tctgccgatt
     2581 ccgctggtca acatccttcc ggcagcggcc attgcctgtc tcgcactggc cctggcggag
     2641 cgggacgggc tctttgccat cattggctat ctgctggcct tgagcacggt cttcatcctg
     2701 ttctatgtct cgacagcgct ctttgctgcg gcaaaggcct tcgttgggac cctgttcggg
     2761 ctgtgaggcc ggacctgaga aaagccggct ttcgggcagc ctcgaggaaa tgcttgtaat
     2821 ccgagtggca agcgactaga tatgcgccat cgagtttcgg ccggttggct cggctccgcg
     2881 ctttcttcat gcaagtgcac tagcccttct cttactccca gaacatcgat ggcccgtatt
     2941 gcgcatgttg cgtgatgcgg aattgcattt gcatcttgaa aggaaagacc atgcagacag
     3001 gtaccgtaaa gtggttcaac gcccagaagg gctttggctt cattgagccg tcttccggcg
     3061 gcccggacgt attcgtccac atcagcgcgg ttgagcgcgc cgggatgacc ggcctcaacg
     3121 aaggtcagaa gatctcctat gagatcgtta ccgaccgccg caatggcaag tctt
//
LOCUS       DBNGVR010000240         2520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4802536, whole genome shotgun sequence.
ACCESSION   DBNGVR010000240 DBNGVR010000000
VERSION     DBNGVR010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4802536"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2414
                     /locus_tag="AC16A0_05200"
     CDS             <1..2414
                     /locus_tag="AC16A0_05200"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR3104762.1"
                     /translation="PDYKQLAQYEPPVMTRIHAADGSLLAEYAEERRLFVPANQMPRQ
                     LINAFISAEDKTFFTHGGLDWMGIASAAVRYVQVKTSGRGQIVGASTITQQVAKNFLL
                     TNERSLQRKLREALLVQRIEQAFSKDQIIELYLNEIFLGLNSYGVAAASLNYFGKSLD
                     QLSLEEIAYLAALPKGPNNYHPFRQRERAIERRNWVLTQMFENGYISEEEMKTAQAKP
                     LEVTPRPFGAQLFAAESFAEEVRRELIEMYGKDKLDKGGLSVRTTLDPKLQIYARQAL
                     ARGLIEFDRKRGYRGPIAKIEIAGDWGPLLKSRKVPADVAPWRIAVVLEVTETEARIG
                     LQPKLKPDGSQVEERETAIIPLEFLAWARAYEDGSKRGPEIKTPADVLAVGDVIYAAP
                     SKENGRYHLVQMPDVEGALVAMDPHTGRVLSLVGGFSYGRSQFNRAVQALRQPGSSFK
                     PIVYAAALDNGYSPASVVMDAPIEFNMPNGEVCKPKNYQNKFFGPSTLRRGIEQSRNV
                     MTVRLADELGMVKVADLSQRLGIYDKLPMQLAMALGAGETTLLKMVTAYAMMDNGGRK
                     IEPTLIDRVQDRYGRTVFRHDARDCTACSAEAYRPDLPEPELLDIREQVMSPYTAYQV
                     TSMMEGVVARGTGKRVQVVGKPVAGKTGTSNEEKDAWFIGFTPDLVVGVYVGYDNPKP
                     MGRGRTGGELAAPIVADFMQLALRDKAATPFRVPRAIELIPIDAKSGRRAIFGKEGVI
                     LEAFKPGDEPPDATKIIGEGTVAQARAPEEGVVLVPQQGGVPTQQVPQDGGSGGLTPQ
                     GGGLY"
BASE COUNT          485 a          822 c          799 g          414 t
ORIGIN      
        1 tccccgacta caagcagctc gcgcagtacg agcccccggt gatgacccgc atccacgcgg
       61 cggatggctc gctgctggct gagtatgccg aagagcgccg gctcttcgta cccgccaacc
      121 agatgccgcg ccagctgatc aatgccttca tctcggcgga ggacaagacc ttcttcaccc
      181 atggcggcct cgactggatg ggtattgcat cggcggctgt acgttatgtg caggtaaaga
      241 cttccggccg aggccagatc gtcggtgcct ccaccatcac ccagcaggtg gccaagaact
      301 tccttctcac caacgagcgc tccctgcagc gcaagctccg cgaggcgctg ctggtgcagc
      361 gcatcgaaca ggccttcagc aaggaccaga tcatcgaact ttatctcaac gagatcttcc
      421 tgggcctcaa ctcctacggt gtggcagccg cctcgctgaa ctacttcggc aagtcgctgg
      481 accagctcag cctcgaagag atcgcctatc tggccgcctt gccgaagggc ccgaacaact
      541 atcatccctt ccgccagcgc gaacgcgcca tcgaacgccg caactgggtg ctgacccaga
      601 tgttcgagaa tggctacatc tccgaggagg aaatgaagac cgcgcaggcg aagcccctgg
      661 aggtgacgcc gcggcccttc ggcgcgcagc tcttcgccgc cgagtccttt gccgaagagg
      721 tgcgccgcga actgatcgag atgtatggca aggacaagct ggacaagggc gggctttcgg
      781 tccgcacgac gctcgatccc aagctgcaga tctacgccag gcaggcgctc gcccgtggtc
      841 tcatcgagtt cgaccgaaag cgcggctacc gcggcccgat cgcaaagatt gagatcgccg
      901 gtgactgggg tccgctgctg aagtcccgca aggtacccgc cgatgtggcg ccctggcgca
      961 tcgcggtggt tctcgaagtc accgaaaccg aggcccgcat cggtttgcag ccgaagctga
     1021 aacccgacgg cagccaggtc gaggagcgcg agacggccat catcccgctc gagttcctcg
     1081 catgggcgcg tgcctatgag gatggttcga aacgcggtcc cgaaatcaag acaccggccg
     1141 atgtgcttgc cgtcggcgac gtcatctacg ccgcgccgtc gaaggaaaat ggcagatatc
     1201 atctcgtgca gatgcccgac gtggagggcg cactggtcgc gatggatccg cacacgggcc
     1261 gcgtgctgtc gctggtgggc ggcttctcct atggacggag ccagttcaat cgcgcggtcc
     1321 aggccttgcg ccagcccggt tcgtccttca agcccatcgt ctatgccgca gcgctcgaca
     1381 atggctacag cccggcctcg gtggtgatgg acgcaccgat cgagttcaac atgccgaacg
     1441 gcgaggtctg taagccgaag aactaccaga acaagttctt cggtccctcg accctgcgcc
     1501 gtggcatcga gcagtcgcgc aacgtcatga cggtgcggct ggccgacgaa ctcggcatgg
     1561 tgaaggtcgc cgacctttcg cagcgcctcg gcatctacga caagctgccg atgcagctgg
     1621 ccatggccct gggagctggc gaaaccacgc tgctcaagat ggtcaccgcc tatgccatga
     1681 tggacaatgg cggcaggaag atcgagccca cgctgatcga ccgtgtgcag gaccgctacg
     1741 gccgcacggt cttccgccac gatgcccgcg attgcacggc ctgctccgca gaggcctatc
     1801 gtcccgatct ccccgaaccg gagcttctcg acatccgcga gcaggtgatg agcccctaca
     1861 cggcctacca agtcacgtcg atgatggaag gtgtcgtcgc ccgcggaacc ggaaagcgcg
     1921 tgcaggtggt gggaaagccg gtggccggca agaccggcac atcgaatgaa gagaaggacg
     1981 cctggttcat cggcttcacg ccggacctgg tggtgggcgt ctatgtcggc tacgacaacc
     2041 ccaagcccat gggcaggggg cgtaccggcg gtgagctggc agcgcccatc gtcgccgact
     2101 tcatgcagct tgcgcttcgc gacaaggccg ccacgccctt ccgcgtgccg cgcgccatcg
     2161 aactcattcc catcgacgcc aagtcgggcc ggcgcgccat cttcggcaag gagggcgtga
     2221 tcctcgaagc cttcaagccc ggcgacgagc cgccggatgc caccaagatc atcggcgagg
     2281 gcaccgtcgc ccaggcgcgt gcgccggagg agggcgtggt attggtgccg cagcagggcg
     2341 gcgtgccaac gcagcaggtt ccgcaggatg gcgggagtgg cgggttgacg ccgcagggcg
     2401 gcggcctcta ttgagcgcca tcaacgcctg aagagacgga ttccatgcgc gccgagatag
     2461 cgaccctggt cgacgaaatg aagcagtcca tcggactgct gaggaggcat ctttgactgg
//
LOCUS       DBNGVR010000241         2909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4016622, whole genome shotgun sequence.
ACCESSION   DBNGVR010000241 DBNGVR010000000
VERSION     DBNGVR010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4016622"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="AC16A0_05205"
     CDS             <1..888
                     /locus_tag="AC16A0_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3104763.1"
                     /translation="SPSIWPVRTDATMLETAVLNLCINARDAMKPKGGRITIETFNRT
                     LDAASCADMEDVPPGDYVEIAVTDTGCGIAAENIEKVFQPFFTTKGPEAGSGLGLSMI
                     YGFAKQSGGHIKIYSEVGHGTTMRLYLPRLLTATDSASHAAPPARDVAAGAGPAMPEV
                     PVVAQVSETVPSKRPLVLVVEDNPSVRDVAAAMIEDMGFDVLIASSGAEGLQAIIERR
                     DIDLVLSDVIMAGGMNGPEMALKALEVRPELKVLFMSGYAPGSLRQMQEELPNAIDLV
                     NKPFTRNDLTEKVKRALAA"
     gene            complement(885..1646)
                     /locus_tag="AC16A0_05210"
     CDS             complement(885..1646)
                     /locus_tag="AC16A0_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR3104764.1"
                     /translation="MTENQPTAAVLLIGDEILSGRTKDKNLGFLADYLTALGIDLKEA
                     RIVADVEAEIVTAVNALRSRYTYVFTTGGIGPTHDDITADAMARAFGVPIGHDPRAVE
                     ILMSYFKANGREPNEARLRMARIPEGATLIANPVSAAPGFQIGNVFVMAGVPKIMNAM
                     MDDVAPRLSRGVPMQTRSIEFLGGEGDAARPLGEIQKQFPSVVIGSYPFQTSEGFGTN
                     LVLRSRDSAVLDEARDAVVAMARQLTAEGKARGWR"
     gene            1854..2675
                     /gene="map"
                     /locus_tag="AC16A0_05215"
     CDS             1854..2675
                     /gene="map"
                     /locus_tag="AC16A0_05215"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3104765.1"
                     /translation="MTYAAAEDVSPRSTRIKLHGPADFEAMRKAGRLTAEALDLLVPL
                     VKPGVTTDELDRVIVEFARDHKAVPAPYNYRGYPKSICTSINHVVCHGIPNDKPLREG
                     DIVNIDVTLVVDGWHGDSSRMYNVGEVNRKAERLVEVTYECLMRGIAVVRPGAHLGDI
                     GHAIQSFAEAERCSVVRDFCGHGLGRVFHDHPNVLHYGRMGEGVELKPGMFFTIEPMI
                     NLGRPHVKILADGWTAVTRDRSLSAQFEHTVGVTETGVESFTLSPKGLHCPPYRL"
     gene            2680..>2909
                     /locus_tag="AC16A0_05220"
     CDS             2680..>2909
                     /locus_tag="AC16A0_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3104766.1"
                     /translation="MAGALRQPRASASPHHAEGHRERLRTRFAAGGPDAIADYELLEM
                     VLFRVIRRGDTKPLAKALLAHFGSFAEVLSASP"
BASE COUNT          535 a          967 c          885 g          522 t
ORIGIN      
        1 tcgccgagca tctggccggt ccgtaccgat gccacgatgc ttgagacggc cgtgctcaat
       61 ctctgcatca atgcccgcga cgccatgaag cccaagggcg ggcgcatcac catcgaaacc
      121 ttcaaccgca cgctggatgc cgcctcctgc gccgacatgg aagatgtgcc gcccggcgac
      181 tatgtggaga tcgccgtcac cgacaccggc tgcggcatcg cggcggaaaa catcgagaag
      241 gtcttccagc cgttcttcac cacaaaggga ccggaggctg gatcgggtct tggcctctcc
      301 atgatctacg gcttcgccaa gcagtcgggc gggcacatca agatctattc ggaagttggc
      361 catggcacca ccatgcgtct ctatctgccg cggctgctga ccgccacaga ctcggcatcc
      421 cacgcagccc cgcccgcgcg cgatgtggcc gccggcgccg gtcctgccat gccggaagtc
      481 cctgtcgtgg cccaggtcag cgagacagtg ccgtccaagc gcccgctggt cctcgtggtc
      541 gaggacaatc catcggtccg ggacgtcgca gcggcaatga tcgaggatat gggctttgac
      601 gtgctgatcg cctccagcgg tgcggaggga ctgcaggcca ttatcgaacg gagggatatc
      661 gacctcgtcc tgtccgacgt catcatggcg ggtggcatga acggccccga aatggcactg
      721 aaggctctgg aggtgcggcc agaactcaag gtcctcttca tgtccggcta tgcgccgggc
      781 tcgctccgcc agatgcagga ggagctgccc aatgcgatcg acctcgtcaa caagcccttt
      841 acccgcaacg atctgaccga gaaggtgaag cgggcgctgg cagcctagcg ccaaccacgc
      901 gcctttccct cggccgtcag ctgccgggcc atggcgacga cggcatcccg ggcctcgtcg
      961 agtaccgcgg agtcgcgcga gcgcagcacc agattggtgc cgaatccctc cgacgtctga
     1021 aatggataag agccgatcac cacactaggg aactgcttct ggatctcgcc cagcggccgc
     1081 gctgcatccc cttccccgcc caggaactcg atgctgcgcg tctgcatcgg aacgccgcgc
     1141 gacagccgcg gcgccacgtc gtccatcatg gcgttcatga tcttgggaac gccagccatg
     1201 acgaagacat tgccgatctg gaagccgggc gcggccgaca ccggattggc gatgagcgtg
     1261 gccccttccg gaatgcgagc catgcgcaag cgcgcctcgt tgggttcgcg gccattggcc
     1321 ttgaaatagc tcatcaggat ctccactgcc cgggggtcat ggccaatcgg cacgccgaag
     1381 gccctggcca tggcatcggc cgtgatgtcg tcatgggtgg ggccgatgcc accagtggta
     1441 aagacatagg tatagcgtga ccgcagtgcg ttgacggcgg tcacgatctc ggcctcgaca
     1501 tccgcgacga tccgggcctc cttcaggtca attcccaagg ccgtcaggta gtcggccaga
     1561 aaacccaggt tcttgtcctt ggtgcggccg gagaggatct cgtcgccaat cagcaaaacg
     1621 gcggcagtcg gctggttctc ggtcattctg gtcaatcccg caggtcccga cccatgtgcc
     1681 accggggcgg atccaagtca aaacctgccg ttggccttgc aggaggccgt cgcggcatct
     1741 atttgggagg tccgaacggc atgttaagcg ggagttgagg ctttcgttcc atgctgtaaa
     1801 ctcccgaccc atcgaaatct accgtccggg cgatcccggt tctggaactc cgaatgacct
     1861 atgctgctgc cgaagacgtg tcgccgcgat ccacccgtat caagctccac gggcctgcgg
     1921 actttgaggc catgcgcaag gccgggcggc tcacggcaga ggccctcgat cttctcgttc
     1981 cccttgtcaa gccgggagtc acgacggacg aacttgaccg cgtcatcgtg gagtttgcac
     2041 gcgatcacaa ggccgtaccc gcgccctaca attaccgcgg ctatccgaaa tcgatctgca
     2101 cctcgatcaa ccacgtcgtc tgccatggca tccccaatga caagccactg cgggagggcg
     2161 acatcgtcaa tatcgacgtg accctcgtcg tcgacggctg gcatggcgat tcgagccgga
     2221 tgtacaatgt gggcgaggtg aaccggaagg ccgaacgcct ggtcgaggtc acctacgagt
     2281 gcctcatgcg cggcatcgcc gtggtcaggc ccggcgccca tctgggcgat atcgggcatg
     2341 ccatccagtc ctttgccgag gccgagcgct gctcggtggt tcgcgacttc tgcgggcacg
     2401 gcctgggccg ggtctttcac gaccacccca atgtcctgca ctacggccgc atgggcgaag
     2461 gcgtcgaact caagcccggc atgttcttca cgatcgaacc gatgatcaat ctcgggcggc
     2521 cgcacgtgaa gatcctggcc gatggctgga ctgccgtgac tcgcgaccgg tctctctcgg
     2581 cccagttcga acatacggtg ggtgtcaccg agaccggcgt cgagagcttc acgctctcgc
     2641 ccaaggggct gcactgcccg ccctatcgcc tctgaggcca tggcaggagc gctccgtcag
     2701 cccagagcct ccgcttcacc gcaccacgcc gaaggtcatc gtgaacgatt gcgcacacgc
     2761 ttcgccgctg gcggacctga cgcaatcgcc gactatgaac tgctcgaaat ggttctgttc
     2821 cgcgtcatcc ggcgtggcga cacgaagccc ctggcgaaag cccttctcgc acattttggc
     2881 agctttgccg aagtcctgag cgcaagccc
//
LOCUS       DBNGVR010000242         4180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1267190, whole genome shotgun sequence.
ACCESSION   DBNGVR010000242 DBNGVR010000000
VERSION     DBNGVR010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1267190"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="AC16A0_05225"
     CDS             <1..627
                     /locus_tag="AC16A0_05225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3104767.1"
                     /translation="ALLEVIMQTHAGMSTEQFLDISKQWLATAEHPRFKRRYDTLIYQ
                     PMLELLVHLRSQGYQTWIVSGGGVEFIRAFAEPVYGIPPQQVVGSSVVTEFQIVDGKP
                     QLMRLPKIDFIDDKAGKPVGINKFIGRRPVFAAGNSDGDLQMLQWTTAGDGPRLGMIV
                     HHTDAEREYAYDRQSSIGRLDKALDQSAANDWLVVDMKNDWTEIFPAM"
     gene            637..2871
                     /locus_tag="AC16A0_05230"
     CDS             637..2871
                     /locus_tag="AC16A0_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3104768.1"
                     /translation="MLRSCLARALLVIGCLLTGASFAVASDVEAFAGSAACSECHADE
                     TAAWAGSHHGWALREPVDGAVLGDFDNATFSHKGIATRFFRDGGRFMVETDGPDGKLV
                     AYPIRYAVGVTPLQQYLVETGNGRLQALDIAWDTVAQKWYHLYPDAEVGAGNGLHWTG
                     SYKNWQARCAACHQTGFDRGFDPKTATYQSKWTELTVSCESCHGPSSAHVAWARQSPR
                     SSPAPQMLKLGPGQQANELAACGPCHARREAFAQKNAPIGAPFGDHYNLSLLTPDLYF
                     GDGQQNAEVFILGSFLQSKMKAKGVTCSNCHEPHAGKLVAEGNAVCTQCHGTAGNAAF
                     PSLALKDYDSEAHTHHKAGSEAAQCVSCHMPDRAYMVNDRRRDHFFRKPDPPQSKAAG
                     APDACTTCHTEKSAEWAAGEIAAWFPDADRRWQDRAALIAFNGGERTQQVFDALAAYA
                     MDLDRPDIVRATTVGALREGTGPQVNAALHALLSDRSELVRAAAVPLFRQVDQQTKLA
                     LLAPLLSDPSRQVRHAAAIELAGGDLAALDAPTREALAKALGEYMDSRLANADMPEAH
                     MALAGLALSMRKWEEAESAFNQAGKIDPQLEQAWLTLARLRAALGDEAGAEAYLQKGL
                     QYLPQGIGLLFERAALEARRGNDARAIDWYRRIIAADSASVDAWLGLGAAALRSRQPA
                     LAIDAAGKAIALAPASADAHVIAAYGFYLQGDLPKARENARRARELAPDLQLPAELDS
                     LLKN"
     gene            3253..>4180
                     /locus_tag="AC16A0_05235"
     CDS             3253..>4180
                     /locus_tag="AC16A0_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR3104769.1"
                     /translation="MTTMKSMMAALFAGTLLAGSCMTATSHAQTQAAAVGAEAVDEEP
                     APLSAEEMEVLVARIALYPDELIAVITAASLYPLQIVEAQRFLDESKTKKDLKPKETW
                     DGSVVSLLNYPEVVKMMSDDLDWTQLLGEAIVNQQKDVLAAIQFLREKAVANGVIKSD
                     DKVKVVKEEDNIVIQPASTEVIYVPQYPPEMLYEPDYIYRPIAYYPDPYPYYYSPIAP
                     FFAGAVTGAIWAGIVDWDDGFWGGNWGNDWGNDIDIDCNKCIIGGDFNGKLDINNVDW
                     KNVDRSKIKFDKNQLNKIDRSKVKNNVKNNDRN"
BASE COUNT          839 a         1317 c         1287 g          737 t
ORIGIN      
        1 gcccttctcg aggtcatcat gcagacacat gccggcatgt cgaccgaaca gtttctcgac
       61 atctccaagc agtggctggc cactgccgaa cacccgaggt tcaagcggcg ctacgatacg
      121 ctgatctatc agcccatgct tgaactgctc gtccatctgc gcagccaggg ctaccagacc
      181 tggatcgtct ccggcggcgg cgtcgagttc atccgcgcct ttgccgaacc cgtctacggc
      241 atcccgcccc agcaggtggt ggggtccagc gtcgtcacgg agttccagat cgtcgacggc
      301 aagccgcaac tgatgcggct gccgaagatc gacttcatcg acgacaaggc cggcaagccg
      361 gtcggcatca acaagttcat cggacggcgc ccggtctttg cggcaggcaa ttcggacggc
      421 gacctgcaga tgctgcagtg gacaacggct ggtgacgggc cgcgccttgg catgatcgtt
      481 catcacaccg atgccgagcg cgagtatgcc tatgatcgtc aatcgtccat cggccgcctc
      541 gacaaggcgc tcgaccagtc cgcggccaat gactggctgg tggtcgacat gaagaatgac
      601 tggaccgaga ttttcccggc gatgtaagct tcgctgatgt tgaggtcttg tctcgcgcga
      661 gcgctcctgg tcatcggttg tcttctgacg ggtgccagct ttgccgtagc atcggatgtc
      721 gaggccttcg cgggcagtgc ggcctgttcc gaatgccatg ctgacgaaac cgcggcctgg
      781 gccgggtctc atcacggctg ggccttgcgg gaaccggtgg acggcgcggt tctgggcgac
      841 ttcgataatg cgaccttctc gcacaagggt atcgcgacgc ggttcttcag ggacggcggc
      901 cgcttcatgg tcgagaccga cggccccgac ggcaagctgg tcgcctatcc gatccgctat
      961 gcggttggcg tcacgccact ccagcagtat ctggtcgaaa ccggcaatgg acggctccag
     1021 gcgctcgaca tcgcgtggga tacggtggcg cagaaatggt accacctcta tcccgatgcg
     1081 gaagtgggtg ccgggaatgg gctgcactgg accggctcct acaagaactg gcaggcacgt
     1141 tgcgcagcct gccaccagac cggctttgac aggggcttcg atccgaagac ggccacctac
     1201 caaagcaagt ggacggagct cacggtctcc tgcgaatcct gtcacggccc gtcgtcggct
     1261 catgtggcct gggcacggca atcgccacgg tcgtcaccgg cgccgcagat gctgaagctc
     1321 ggacccggac aacaggccaa tgaactggcc gcctgcgggc cttgccatgc gcggcgcgaa
     1381 gctttcgcgc agaagaacgc accgatcgga gcgcctttcg gcgaccacta taatctatcg
     1441 ctgctgactc ccgatcttta cttcggtgac ggccagcaga atgccgaggt tttcattctc
     1501 gggtccttcc ttcagtccaa gatgaaagcc aagggggtta cctgttccaa ctgccacgaa
     1561 ccccatgccg gaaagctggt cgccgaaggc aacgccgtct gcacgcagtg ccacggcacg
     1621 gcaggcaatg cggccttccc cagtcttgcg ctcaaggact atgacagcga ggcgcatact
     1681 catcacaagg ctggcagcga ggccgcgcaa tgcgtcagct gccacatgcc tgaccgcgcc
     1741 tacatggtga acgaccgccg ccgcgaccac ttcttccgca agcccgatcc gccgcagtcc
     1801 aaggccgcag gcgcaccgga tgcctgtacg acctgccaca ccgagaagtc ggccgaatgg
     1861 gctgccggag agattgccgc gtggttcccg gatgccgacc ggcgctggca ggatcgcgcg
     1921 gccctgatcg ccttcaatgg cggcgaaagg acccagcagg tcttcgatgc gctggccgcc
     1981 tatgccatgg atctcgatcg ccccgacatc gtacgcgcaa cgacggtcgg ggctctgcgt
     2041 gaaggtactg gcccgcaggt gaatgccgcg ctccatgcgc ttctgtccga tcgcagcgaa
     2101 ctggtccggg ccgctgcggt acctctgttc cggcaggttg atcagcagac gaagcttgcc
     2161 ctcctggctc cgctgctcag cgatccgtca cgacaggtgc ggcatgcggc cgcgatcgag
     2221 cttgccggcg gcgaccttgc cgccctcgac gcgcccacgc gtgaggcgct ggccaaggcc
     2281 ctcggcgaat acatggactc gcggctggcc aatgccgaca tgccggaagc acatatggcg
     2341 ctggcgggac tcgcgctcag catgcgcaag tgggaagagg ccgagtcggc attcaatcag
     2401 gccggcaaaa tcgatccgca actcgagcag gcctggctca ccctagcgcg gctgcgcgca
     2461 gccctcgggg acgaggccgg cgcggaggcc tatctgcaga aggggttgca atatctgccg
     2521 caaggcatcg gactgctctt cgagcgcgcc gcgctcgaag cccggcgcgg caacgacgcg
     2581 cgcgccatcg actggtaccg ccggatcatc gctgccgact ccgccagcgt cgatgcctgg
     2641 cttggcctgg gcgccgcggc cttgcgcagc cgccagccgg cactggccat cgatgcggcc
     2701 ggcaaggcaa tcgcattggc gcccgcctcg gctgacgcac atgtgatcgc cgcctacggc
     2761 ttctaccttc agggggacct gccgaaggca agggagaatg cccgccgggc gcgcgaactg
     2821 gcgcctgacc tgcagcttcc ggctgagctc gacagtctgc tgaagaatta aagcttcgcc
     2881 atcagggctg atctgcttga cgttgcgctt gatttgcgcc atgcaaaacg caactcgatc
     2941 aacatacttt gtgtaaacaa ggtatttcta ggcggggata gacaatcgtg tggcctagtg
     3001 aaggccttgc ggaactgccg atcagcacgg cgaaagcctt ggtcagccat cgagatctgg
     3061 agccacttcg gcgagattgt cgaaatccgg acaatctccg tccaagttct atgctctatc
     3121 gccacagaat tgacaggtgg ccgataaggc ctgagggaac gggttcgggc gggacctcct
     3181 caatcacagg taatgacagg cagtatggcc atcagccagc tcgccgagag tattgaactg
     3241 ggagttgcag agatgacgac aatgaaatcc atgatggcgg ccctgttcgc aggaacattg
     3301 cttgccggaa gctgcatgac tgcaaccagc catgcccaga cccaggcggc ggcggtcggc
     3361 gccgaagcgg tggacgagga gcctgcacct ctcagtgccg aggagatgga agtgctcgtc
     3421 gcgcggatcg cgctttatcc tgacgaattg atcgctgtca tcaccgcggc ctcgctctat
     3481 cccctgcaga tcgtcgaagc ccagcgcttc ctcgacgaaa gcaagacgaa gaaggatctg
     3541 aagccgaagg agacgtggga cggcagcgtc gtttcgcttc tgaactatcc cgaagtcgtc
     3601 aagatgatga gcgacgatct cgactggacg cagctgcttg gcgaagcgat cgtcaaccag
     3661 cagaaggacg tactcgcggc gatccagttc ctgcgcgaga aggccgttgc caacggtgtc
     3721 atcaagtccg acgacaaggt gaaggtggtc aaggaagagg acaacatcgt catccagccg
     3781 gccagcaccg aagtcatcta tgtcccgcag tatccgccgg agatgctcta tgagcccgac
     3841 tacatctacc ggcccatcgc ctactatccc gatccctatc cctactacta cagcccgatc
     3901 gcgccgttct tcgcaggcgc cgtgacaggc gccatctggg cgggcattgt cgactgggat
     3961 gacggcttct ggggcggcaa ctggggcaat gactggggca acgacatcga cattgattgc
     4021 aacaagtgca tcatcggtgg cgacttcaac gggaagctcg acatcaacaa tgtcgactgg
     4081 aagaacgtcg atcgcagcaa gatcaagttt gacaagaacc agctcaacaa gatcgaccgg
     4141 tcgaaggtca agaacaacgt caagaacaac gaccgcaaca
//
LOCUS       DBNGVR010000243         4415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_87721, whole genome shotgun sequence.
ACCESSION   DBNGVR010000243 DBNGVR010000000
VERSION     DBNGVR010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_87721"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC16A0_05240"
     CDS             <1..192
                     /locus_tag="AC16A0_05240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3104770.1"
                     /translation="DVHEAPQIMRGNAQTLEPGMVFTIEPGLYRDGEAGVRIEDDVVV
                     TETGIECLTSFPRDLRLVG"
     gene            265..2118
                     /gene="lepA"
                     /locus_tag="AC16A0_05245"
     CDS             265..2118
                     /gene="lepA"
                     /locus_tag="AC16A0_05245"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR3104771.1"
                     /translation="MTDTAHIRNFSIIAHIDHGKSTLADRLIQLCGAVSDREMVNQIL
                     DSMDLERERGITIKANTVRLDYKARNGENYVLNLIDTPGHVDFAYEVSRSLAACEGSL
                     LVVDASQGVEAQTLANVYQAINNNHEIVPVLNKVDLPAADPDRVRQQIEDVIGIDASD
                     AIPISAKTGVGVPEVLEAIVHRLPAPKGDRNAPLKALLVDSWYDSYLGVVVLVRIIDG
                     VMKKGQKVKLVSTNAVYQLDRVGVSRPKREMVDELGPGEIGFFTASIKQVADTRVGDT
                     VVEDKAQAVTPLPGFRPAQSVVFAGFFPVDAARFEDLRDAMGKLRLNDASFSYDMETS
                     AALGFGFRCGFLGLLHLEIIRERIEREFNVDIITTAPSGIYKVNMRDGAQIELHNPVD
                     LPDQTLILSIEEPWIEATILVPDEYLGAILKLCEDRRGRQKQLTYAGARAMLVYELPL
                     NEVVFDFYDRLKSVSRGYASFDYKMIGYEEGDLVRLAILVNAEPVDALAMIVHRARAE
                     QRGRVMCEKLKDLIPRHMFQIPIQAAIGGKIVARETLSALRKDVTAKCYGGDISRKRK
                     LLDKQKEGKKKMRQFGKVEIPQEAFISALKMDENLSSASPTRLTLSSRPAQ"
     gene            complement(2048..3160)
                     /locus_tag="AC16A0_05250"
     CDS             complement(2048..3160)
                     /locus_tag="AC16A0_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3104772.1"
                     /translation="MNSTTYSAHAGTGSLRLGHRAIETLRTLAHGVRTHATLYVLAIG
                     TYLIGLLQSLWLGVPLSFGLVSIVGESTFAFLVLFILLWLAGDFIRLWWTGYAGSPTR
                     ALTSRLLDDILAPGRVSNTIHAFIINGVFFVGFLTIKKNIPITVPFSWDQSFMELDRF
                     LHFGLLPHEILAPLLQYPPVTFVLNITYNMWFVVMMAFFMWQGFRKRDTALRQQYLTS
                     YLLTWLIGTCVIGTLLSSAGPCFYGFVVDGPDPYAGLNAYLAQANTIYPIWAVPTQAL
                     LWQIHMAGFGDVEGVSAMPSMHVATTVLFICCAVASGKRWLIWFTSIFSGVILIGSVM
                     LSWHYAVDGYLGAVIELACWKASVWWVKRSLSSRPS"
     gene            3308..4264
                     /gene="mbfA"
                     /locus_tag="AC16A0_05255"
     CDS             3308..4264
                     /gene="mbfA"
                     /locus_tag="AC16A0_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron exporter MbfA"
                     /protein_id="HIR3104773.1"
                     /translation="MKSFSDLTEQELLALAISSEDDDARAYAGFAHSLMDLYPASAKV
                     FMEMAEEEHQHRRWLTDLYQKKFGDFIPLIRRGDIQGFVRHKPAWTIGKMNIEEMRQR
                     AEVIELENYRFYKQAAERSQDPQVKKLLLDLAEAEKGHESIAVKLGLTHLTADAKIEE
                     GEAKRRLFLLQVIQPGLVGLMDGSVSTLAPLFAAAFATGNTWETFLVGSAASIGAGIS
                     MGLAEGLSDDGQLTGRGHPLIRGLAAGVMTAVGGLGHTLPYLINDFQIATWIAIAVVF
                     VELWAISWIRYKYMDTPFLKAAFQIAVGGFIVFVTGILIGSA"
BASE COUNT          905 a         1373 c         1361 g          776 t
ORIGIN      
        1 gatgtccacg aggcgccgca gatcatgcgc ggcaacgccc aaaccctgga acccggcatg
       61 gtcttcacca tcgagccggg cctctatcgc gacggcgagg ccggtgtccg catcgaggat
      121 gacgtggtcg tcaccgaaac cggcatcgag tgcctgacca gcttcccgag ggatctccgg
      181 ctggtgggct gacggccgga cctcaggggc ctgcgccgcg ccgcttgacc ataggccgcc
      241 gccctgtcaa acaccgcgcc aaccatgacc gataccgccc acatccggaa tttctccatc
      301 atcgcccata tcgaccacgg caagtccacg ctggccgacc ggttgattca gctctgcggc
      361 gccgtatccg accgcgagat ggtgaaccag atcctcgact cgatggacct cgagcgggaa
      421 cgcggcatca ccatcaaggc caatacggtg cgcctcgact acaaggcccg caatggcgag
      481 aattacgttc tgaacctgat cgacacgcct ggtcacgtcg acttcgccta tgaggtctcg
      541 cggtcgctgg ccgcctgcga gggctcgctc ctcgtggtcg acgcctcgca gggtgtcgag
      601 gcccagacgc ttgcgaatgt ctaccaggcg atcaacaaca atcacgagat cgttcccgtc
      661 ctcaacaagg tcgaccttcc ggccgccgat ccggaccgcg tgcgccagca gatcgaggac
      721 gtgatcggca tcgatgcctc ggatgcgatt ccgatctcgg ccaagaccgg tgtcggcgtg
      781 cccgaggttt tggaagccat cgtgcaccgc ctgccggcgc ccaagggcga ccgcaacgcg
      841 ccgctcaagg cccttctcgt cgacagctgg tacgacagct atctcggcgt cgtggttctg
      901 gtccgcatca tcgatggcgt catgaagaag gggcagaagg tgaagctcgt ctccaccaat
      961 gccgtctatc agcttgaccg tgtcggcgtg tcgcgcccca agcgcgaaat ggtggacgag
     1021 ctcgggcccg gcgaaatcgg attcttcacc gcctcgatca agcaggtggc agatacgcgc
     1081 gtcggcgata ccgtggtcga ggacaaggcc caggccgtca cgccgctgcc gggcttccgc
     1141 ccggcccagt cggtggtctt cgcgggcttc ttccccgtcg atgcggcgcg cttcgaggac
     1201 ttgcgcgacg ccatgggcaa gcttcgcctg aacgatgcga gcttctccta cgacatggaa
     1261 acctctgccg cgctcggctt cggattccgc tgcggctttc tcggcctgct tcacctcgag
     1321 atcatccgtg agcggatcga acgcgagttc aacgtcgaca tcatcaccac cgcgccatcg
     1381 ggcatctaca aggtgaatat gcgcgacggc gcccagatcg agctgcacaa tccggtcgat
     1441 cttccggacc agacgctgat cctgtcgatc gaggagccct ggatcgaggc caccatcctg
     1501 gtgccggacg agtatctcgg tgccattctc aagctctgcg aggaccgccg cggccgccag
     1561 aagcagctca cctatgccgg cgcacgcgcc atgctggtct acgagcttcc gctcaacgag
     1621 gtggttttcg acttctacga ccggctcaag tcggtgtcgc gcggctatgc atcgtttgac
     1681 tacaagatga tcggctacga agagggcgat ctcgtccggc ttgccatcct ggtgaacgcc
     1741 gagccggtcg atgcgctcgc catgatcgtg caccgggccc gcgccgagca gcgtggccgg
     1801 gtcatgtgcg agaagctcaa ggaccttatt ccccgccaca tgttccagat cccgattcag
     1861 gcggcgatcg gcggcaagat cgtggcgcgc gaaactctct cggcgctgcg caaggacgtc
     1921 accgcaaagt gctacggtgg cgacatcagc cgaaagcgca agctcctcga caagcagaaa
     1981 gagggcaaga agaagatgcg gcagttcggc aaggtcgaaa tcccgcagga ggccttcatc
     2041 tcggccctca agatggacga gaacttaagc tccgcttcac ccaccagact gacgctttcc
     2101 agcaggccag ctcaatgacc gcgccgagat agccatcgac ggcatagtgc cagctcagca
     2161 tcaccgatcc gatcaggatg acgcctgaga agatgctggt gaaccagatc agccagcgct
     2221 tgcccgatgc caccgcgcag cagatgaaca gcaccgttgt cgccacgtgc atcgacggca
     2281 tggcagagac tccttcgacg tcgccgaagc cggccatatg gatctgccag agcagcgcct
     2341 gcgtcggcac cgcccagatc ggatagatgg tattggcctg ggccagatag gcattgaggc
     2401 cggcataggg gtcggggcca tcgaccacga agccgtagaa gcaaggaccc gccgaggaca
     2461 gaagcgttcc aatcacgcag gtgccaatga gccaggtcag gagataggag gtgaggtact
     2521 gctgacgaag cgccgtgtcg cgcttgcgga agccctgcca catgaagaag gccatcatca
     2581 ccacaaacca catgttgtag gtgatgttca gcacgaaggt caccggcgga tactgcagaa
     2641 gcggcgccag gatctcgtgc ggcagaagtc cgaagtgcag gaacctgtcg agctccatga
     2701 agctctggtc ccaggaaaag ggcacggtga tgggaatgtt cttcttgatg gtcaggaagc
     2761 ccacgaagaa gacgccgttg atgatgaagg catggatcgt gttggacacg cgcccagggg
     2821 ccaggatgtc gtccagcagg cgcgaggtca gtgcgcgggt cgggctcccc gcatagccgg
     2881 tccaccacag ccggatgaag tcgccggcca gccacagcag gatgaagagg accaggaagg
     2941 cgaaggtcga ctcgccgacg atcgacacga gaccgaagga cagggggacg cccagccaca
     3001 gactctgcag caggccgatc aggtaggtgc cgatggcgag gacatagagc gtcgcatgcg
     3061 tccggactcc gtgtgcgaga gtccggagcg tctcgatcgc gcggtggcca agcctcaggg
     3121 aaccggtccc ggcatgagcg ctgtaggtcg tggaattcat ggtcccgcca tcctctttgg
     3181 ccggaactat ctgaggagcg gggttaagtt caggttgtga ggaacacgca agctgaactg
     3241 gacctcagtt tagaattatt ctaaggttaa gacatcgttt cgacggcagc gaaagacagg
     3301 attgaacttg aagagttttt cggaccttac agaacaggaa ttgctggcac tcgcgatctc
     3361 cagcgaggac gatgatgcca gggcctatgc cggatttgcc cattcgctca tggatctcta
     3421 tcccgcctcg gccaaggtct tcatggaaat ggcggaggag gagcatcagc atcgccgctg
     3481 gctgaccgac ctctatcaga agaagttcgg cgatttcatc ccgctcattc gccgcggcga
     3541 catccagggc ttcgtccgcc acaagccggc ctggaccatc ggcaagatga acattgagga
     3601 gatgcggcag cgcgccgagg tgatcgagct cgagaactac cgcttctaca agcaggccgc
     3661 cgaacgcagc caggaccctc aggtgaagaa gctgctgctc gacctggcgg aagccgagaa
     3721 aggtcatgaa tcgatcgcgg tgaaactcgg cctcacgcat ctgacggcgg atgccaagat
     3781 cgaggagggc gaggccaagc gccgcctgtt cctgctgcag gtgatccagc cgggccttgt
     3841 tgggcttatg gatggctctg tctccacact ggccccgctg tttgccgcgg cctttgccac
     3901 tggcaacacg tgggaaacat tcctggtcgg ttccgcagcc tcgatcggtg ccggcatttc
     3961 catgggactt gccgagggcc tctcggatga tggccagctc accgggcgcg ggcacccgct
     4021 catccgcggc ctcgccgccg gcgtgatgac ggccgttggt ggtctcgggc atacattgcc
     4081 ctatctgatc aatgacttcc agattgcgac ctggatcgcg atcgccgtcg tcttcgtcga
     4141 actctgggcg atttcatgga ttcgctacaa gtatatggac acgccgttcc tcaaggcagc
     4201 cttccagatc gcggtcggcg gtttcatcgt gttcgtcacg ggcatattga tcggaagcgc
     4261 ctgacggaaa tcaggcaatc cgcagcagaa cggctcccgc cacgatcaga acggagcctg
     4321 caacgcggat tgccgtcacg ctttccttga gaacgcgcat cgacagcaga atgacgaaga
     4381 aaatgctggt ttcgcgcaac gccgccacgg cggca
//
LOCUS       DBNGVR010000244         2563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4803263, whole genome shotgun sequence.
ACCESSION   DBNGVR010000244 DBNGVR010000000
VERSION     DBNGVR010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4803263"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="AC16A0_05260"
     CDS             complement(<1..453)
                     /locus_tag="AC16A0_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3104774.1"
                     /translation="MTAKTNLTINPQRLWDALMETATFGGTPKGGIKRLTVSDEDKKV
                     RDWFKAECEKLGCAVVVDEVGNMFATRPGRRKDLAPIAMGSHLDTQPTGGKFDGVLGV
                     LGALEAMRTLVDMGYETNAPVMIVNWTNEEGSRFAPAMLCSGVYAGVFT"
     gene            complement(470..1540)
                     /locus_tag="AC16A0_05265"
     CDS             complement(470..1540)
                     /locus_tag="AC16A0_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3104775.1"
                     /translation="MEYRQLGRSGLRVSLHTLGTMTFGGEGFFAMAGKTALDEARRII
                     DVAVDHGVNMIDTSNIYSVGRSEEIVGAALKDKSQQILVGTKVRFSMGQGPNEGGLSR
                     YHIIEQCEASLKRLQRDRIDLYFLHEWDGQTPVEEMMEALDTLVKQGKVRYIGCSNFS
                     GWHIMKALMAADRHNWQRFCCQQIHYTIEAREAEHELMPIAIDQGVGIQVWSPIAGGL
                     LSGKFRRNAPPPQISRHTAGWGEPPIADEDRLYRIIDALVAVGEARGVSAAQVALAWL
                     AGRPGVASIIVGARTEAQLKDNLAAAGLELTPEERKRLDDVSLRPLPYPYWHQCNTAA
                     DRLGPADLALHAPHLANRKPRM"
     gene            complement(1656..2138)
                     /locus_tag="AC16A0_05270"
     CDS             complement(1656..2138)
                     /locus_tag="AC16A0_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3104776.1"
                     /translation="MTTNTICIWYDGAAEAAARFYASVFPDSHVDGMHLAPADYPSGR
                     KGDVITVEFTVVGVRCLGLNGGPAFPQTEAFSFQIATGSQEETDRYWNAIVGNGGRES
                     ACGWCKDKWGVNWQITPRVLIEALAAGGAPARRAFEAMMTMGKIDVAAIEAAVRGTPG
                     "
     gene            2139..>2563
                     /locus_tag="AC16A0_05275"
     CDS             2139..>2563
                     /locus_tag="AC16A0_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104777.1"
                     /translation="MALQIIVGSIVPSRMPPYGNAAQPKFRHDDCAAILYPDRKAVRR
                     QSMISSNPSQLAMAGSKSASELAFLHIGKNAETQIMFLAKQLKPHGVTVHQLPHDAKL
                     CRLPEKLPYFFSIRNPISRFKSGFYSRKRKGQPRLYVEWS"
BASE COUNT          418 a          853 c          828 g          464 t
ORIGIN      
        1 ggtgaagacg ccggcataga cgcccgagca cagcatggcc ggcgcgaagc gcgagccttc
       61 ctcgttggtc cagttgacga tcatcaccgg cgcattggtc tcgtagccca tgtcgaccag
      121 cgtgcgcatg gcctcgagcg cgccgagcac gcccagcacg ccgtcgaact tgccgccggt
      181 gggctgggtg tcgagatgcg agcccatggc gatgggcgcc aggtccttgc gccggcccgg
      241 ccgggtggcg aacatgttgc ccacctcgtc gaccacgacc gcgcagccca gcttctcgca
      301 ttcggccttg aaccagtcgc gcaccttctt gtcttcgtcc gacacggtga ggcgcttgat
      361 gccgcccttg ggcgtgccgc cgaacgtcgc ggtctccatc agcgcgtccc agaggcgctg
      421 cggattgatg gtgagattgg tcttcgctgt catgacggac tccggcggct tacatgcgcg
      481 gcttgcggtt ggcgagatgc ggcgcgtgca gcgccagatc ggcaggcccc agccggtcgg
      541 ccgccgtgtt gcactggtgc caatagggat agggcaaggg cctgaggctc acgtcatcga
      601 gccgcttgcg ctcctcgggc gtcagctcca gcccggcggc ggcaagattg tccttgagct
      661 gcgcctcggt gcgcgcaccc acgatgatgg aggcaacccc cggacgcccg gcgagccagg
      721 ccagcgccac ctgcgcggct gagacgccgc gcgcctcgcc caccgcgacc agcgcatcga
      781 tgatgcggta gagccggtcc tcgtcggcga tggggggctc gccccagcct gcggtatggc
      841 gcgagatctg cggcggcggc gcattgcggc ggaacttgcc cgagagcagc ccgccggcga
      901 tcgggctcca gacctggatg cccacgccct ggtcgatggc gatgggcatc agttcgtgct
      961 cggcctcgcg ggcttcgatc gtgtagtgga tctgctggca gcagaagcgc tgccagttgt
     1021 ggcggtcggc ggccatcagg gccttcatga tgtgccagcc ggagaagttg gagcagccga
     1081 tgtagcggac cttgccctgc ttcaccagcg tgtcgagcgc ctccatcatc tcctcgaccg
     1141 gcgtctgccc gtcccattcg tggaggaaat agaggtcgat gcggtcgcgc tgcaggcgct
     1201 tgaggctcgc ttcgcactgc tcgatgatgt ggtagcgcga gaggccgccc tcgttcggtc
     1261 cctggcccat ggagaagcgt accttggtcc cgaccaggat ctgctgcgac ttgtccttca
     1321 gcgccgcgcc gacgatctcc tccgagcggc cgacggaata gatgttggaa gtgtcgatca
     1381 tgttgacgcc gtggtccacc gccacgtcga tgatgcgcct ggcttcgtcg agcgccgtct
     1441 tgcccgccat cgcgaagaag ccttcgccgc cgaaggtcat cgtgcccagc gtgtgcagcg
     1501 acacacgcag gcccgaacgg ccgagttgcc gatattccat gactgactcc ctgaattgat
     1561 cggccttccc tatgccatat cctgcggtcg cagggaaatg caggtgagac atggtctgcc
     1621 tgcgccgggg ccggggattg cgcctggccc gccgattacc cgggtgtgcc gcgcaccgcc
     1681 gcctcgatcg ccgcgacgtc gatctttccc atggtcatca tggcttcgaa ggcccgccgg
     1741 gccggagcac ccccggcagc cagcgcctca atgaggacgc gcggcgtgat ctgccagttt
     1801 acgccccact tgtccttgca ccagccacag gcgctttcgc ggccgccgtt accgacgatc
     1861 gcattccagt agcggtcggt ttcttcctgg ctgccggtcg cgatctggaa cgagaaggct
     1921 tccgtctgcg ggaaggctgg cccgccgttg aggcccaggc agcgcacgcc caccacggtg
     1981 aattccaccg tgatcacgtc gcccttgcgg ccggacggat agtcggcggg cgcaagatgc
     2041 atgccgtcga catggctgtc gggaaagacc gaggcataga agcgggcggc cgcttcggcc
     2101 gcaccgtcat accagatgca gatcgtgttg gtggtcatgt ggcgctccag atcatcgtcg
     2161 gttccatcgt tccgtcgagg atgccaccat acggcaacgc cgcacagccg aaattccgcc
     2221 atgacgattg cgccgccatc ctctatcctg accgcaaggc agtccgaaga cagagcatga
     2281 tcagcagcaa tcccagtcag cttgccatgg caggcagcaa atccgcatcc gaactggcgt
     2341 tcctccatat cggcaagaat gcggaaacgc agatcatgtt ccttgccaag cagctcaagc
     2401 cgcatggcgt gacggtccac cagctgccgc atgacgcgaa gctttgcagg ctcccggaga
     2461 agctgcccta tttcttttcg atccggaatc cgatcagccg gttcaagtcg gggttctatt
     2521 cacgcaagcg gaaaggccag ccccggcttt atgtggaatg gtc
//
LOCUS       DBNGVR010000245         2156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_284873, whole genome shotgun sequence.
ACCESSION   DBNGVR010000245 DBNGVR010000000
VERSION     DBNGVR010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_284873"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="AC16A0_05280"
     CDS             <1..504
                     /locus_tag="AC16A0_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3104778.1"
                     /translation="RDKAQRTPLLAATRANAIDAARLLIEAGANVNAKDNIRDTPFLY
                     AGAEGRLEILRMTLKAGANLKDTNRYGGNALIPACHHGHPETVRELLKTDIDIDHINR
                     LNWTALLETIILSDGGPTHQDILAQLIAAGANVNIADGDGISPLAHAKQRGYAEMVVM
                     LEEAGAH"
     gene            complement(501..1796)
                     /locus_tag="AC16A0_05285"
     CDS             complement(501..1796)
                     /locus_tag="AC16A0_05285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3104779.1"
                     /translation="MAYGSLALSSNASFDPFWWRAAPREEAAPGVPPRETDVAIVGSG
                     MTGLVAALHLARGGRKVTVLEAREPGHGASTRNAGYVGRTLKHSFGEIMETEGLDQAK
                     RVYGELMDAFMAVKETVERERIDCHYRQQGRLLLATSSAMHEAMAREFALREVHLGEA
                     FETVTRDLQRNEIATDHYFGGVRIPDHAGLHPGLYHQGLLDAARTAGVQVCAHAPVLG
                     FRREPRGFTVSLKGARVEARDLIFATNGYGGSAWPWLMRRLLPFHAYQAVSAPLGENR
                     IASLLPGDRTFIDWNFNVDWMRRAPGDPTRIVFGGLTGEIDADLRVMAERLQLRLLRI
                     FPDLSDLRFDHVWTGRCSGTFDINPRIGVQDGVHFAGGYCFAGVPMGTLFGQKLARRI
                     LGQKGGESVFDRPLETRFFYRGNAWFVPHAIRWMSRNDR"
     gene            complement(1798..>2156)
                     /locus_tag="AC16A0_05290"
     CDS             complement(1798..>2156)
                     /locus_tag="AC16A0_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3104780.1"
                     /translation="RMQRDMVSVYPQLGNPRIDFAWSGIMGYARHFMPLIGTDGDGQW
                     WATAFGGHGMNTTAMGGILISRAIAAKDDEYRRFLPFAPDWAGGPLGRVAVQLGYWQM
                     QLRDRLDEARASRKAA"
BASE COUNT          405 a          726 c          687 g          338 t
ORIGIN      
        1 cgcgacaagg cccagcgtac gccgctgctg gcggccaccc gcgcaaatgc cattgacgct
       61 gcccgcctgc tgatcgaggc gggtgccaat gtgaatgcca aggacaatat ccgcgacacg
      121 cccttcctct atgccggtgc cgagggccgc ctcgagatcc tgcgcatgac cctgaaggcc
      181 ggcgcaaatc tcaaggacac caaccgctat ggcggcaatg ccctgatccc ggcctgccac
      241 cacggccatc ccgagacggt gcgcgaactg ctcaagacag acatcgacat cgaccacatc
      301 aaccggctga actggacggc gcttctggaa accatcatcc tttcggacgg cgggccgacg
      361 catcaggaca tcctcgcgca gctgattgct gccggcgcca acgtcaacat cgcggatggc
      421 gacggcatct cgccgctcgc ccatgcgaaa cagcgtggct atgccgagat ggtcgtcatg
      481 ctggaagaag ccggagcgca ctagcggtcg ttgcggctca tccagcggat ggcatggggc
      541 acgaaccagg cgttgccccg atagaagaag cgcgtctcga gcggccggtc gaagacgctc
      601 tcgccgccct tctgcccaag aatgcgccgg gccagcttct gcccgaagag cgttcccatc
      661 ggcacgcccg cgaagcagta gccgccggcg aagtggactc cgtcctgcac gccgattcgc
      721 ggattgatgt cgaaggtgcc gctgcagcgg ccggtccaca catgatcgaa tcgcaggtca
      781 ctgagatcgg ggaagatgcg cagcagccgt agctgcaggc gttcggccat gacacgcaga
      841 tctgcgtcga tctcgccggt aaggcccccg aagacgatac gcgtcggatc gcccggtgca
      901 cggcgcatcc agtcgacgtt gaaattccag tcgatgaagg tgcggtcgcc tggcagcaat
      961 gacgcaatgc gattctcgcc gagcggtgcc gagaccgcct ggtaggcatg gaacggcaga
     1021 aggcgccgca tcagccatgg ccaggctgag ccgccataac cattggtggc gaagatcaga
     1081 tcacgcgcct caacacgtgc acccttgagg gatacggtga agcccctggg ttcccggcga
     1141 aagccgagaa cgggtgcatg tgcacagact tgtacgccgg ccgtgcgggc cgcgtccagc
     1201 agcccctggt gatagagccc gggatgaagc cccgcatgat cagggatgcg gacaccgcca
     1261 aagtaatgat ccgtggcgat ttcgttgcgc tgaagatcgc gcgtcactgt ctcgaaggct
     1321 tcgccgagat gaacctcgcg cagggcgaac tcgcgcgcca tggcctcatg catggccgaa
     1381 gacgtggcga gcagcagccg gccctgctgg cggtagtggc agtcgatcct ttcccgctcc
     1441 accgtctcct tgacggccat gaaggcatcc atcagttcgc catagacgcg cttcgcctga
     1501 tcaagaccct cggtttccat gatctcgccg aaactgtgct tcagcgttcg cccgacataa
     1561 ccggcgttgc gcgtgctggc accgtggccc ggctcgcgcg cctcaaggac cgtcaccttg
     1621 cggccaccgc gtgccagatg cagcgccgcc accagtcctg tcatgccgga tccgacgatc
     1681 gccacatcgg tctcgcgcgg cggcacaccc ggtgcggcct cctcgcgcgg ggccgcacgc
     1741 caccagaagg gatcgaagga ggcgttggaa ctgagcgcca ggcttccata ggccatgtca
     1801 ggccgccttc cggctggcgc gggcttcgtc cagccggtcg cgcagctgca tctgccaata
     1861 gccgagctga acggcgacac gccccagggg accccccgcc cagtccggcg cgaagggcag
     1921 gaagcggcgg tattcgtcgt ccttggcggc gatggcgcgc gagatgagta tgccacccat
     1981 ggccgtcgtg ttcatgccgt ggccgccgaa cgcggtggcc caccactgcc catcgccgtc
     2041 ggtgccgatc agcggcatga agtggcgcgc atagcccatg atgccgctcc aggcgaagtc
     2101 gatgcgcgga ttgccgagct gcggatagac cgaaaccatg tcgcgctgca tgcggg
//
LOCUS       DBNGVR010000246         4325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_481807, whole genome shotgun sequence.
ACCESSION   DBNGVR010000246 DBNGVR010000000
VERSION     DBNGVR010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_481807"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1095)
                     /locus_tag="AC16A0_05295"
     CDS             complement(118..1095)
                     /locus_tag="AC16A0_05295"
                     /inference="COORDINATES: protein motif:HMM:NF020932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HIR3104781.1"
                     /translation="MLKPMLAGLSVLTLALVPAQAADSPDRLYDRIVAEVVAPGFGNL
                     ASAASRHAADWQSFCAAPSEALREGLVASFNDTADRWAEVEIFRSGPSSRDFRHERFH
                     FWPERKNAVSRGLAALRADSAAATPAQVSAASAAVQGLPALERLIFNDEHAADRCELG
                     PAIATNAAELAGQMQVAWTTAAPSASAESRTALATDLVTSFAIIKDSKIEAVIGKTPA
                     DVKPRAAESWRSGRSFRNIAHNLEGLARVNSVLASSLPEDAALVSTTRQAQRIATGLS
                     GDLGAIAVSDRRFEAILLRDAVDAAEDRAAIEIPAALGVTIGFNSLDGD"
     gene            complement(1105..2289)
                     /locus_tag="AC16A0_05300"
     CDS             complement(1105..2289)
                     /locus_tag="AC16A0_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018269.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HIR3104782.1"
                     /translation="MRRFPVSLVAVLAMAATAAADPLDTAMGKALFARNWVAAPASTD
                     ASDGLGPLFNARSCNACHAGGGPARFETDNGWFKARGLVVRSGASDGRPHPLLGAQLQ
                     TAALPGLAAEARVAVRLRGKALVVETRLQEPDDAIVLETRIAPSLRGRGAIDRIDETA
                     ILALADPSDRDGDGLSGRANMIADGGGRLVPGRFGSKATGANLLQQTAAAAALDLGLS
                     SPLVPRPHGDCTSDQADCLAHATGRSAITANEEISADMVRLITAYVATLEPRKPDTAV
                     RGFEIFRDSGCSGCHVPQLPDRHGMPVTIFSDLLLHDMGDATSSALRDGSALPGEWRT
                     APLIDLDPRQGTRRYLHGGKAATIEDAILTHGGEAARARDRFRQLDGRERQLLLDFLA
                     QL"
     gene            complement(2286..3569)
                     /locus_tag="AC16A0_05305"
     CDS             complement(2286..3569)
                     /locus_tag="AC16A0_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HIR3104783.1"
                     /translation="MKFPWLGLMAGTALTLSAAFSLALGDTATPKAVATHYADMAEAM
                     YGDARAAAADLQKAVDALVAKPSQETLETARKAWRESRPWYQQTEGFRFGNAIVDDWE
                     GRVNAWPLDEGLIDYVNIATYGEESDENVLYAANVIANPKLTIGTETIDASAITPDLI
                     KSLHEAADSEANVSVGYHAIEFLLWGQDLNGTGPGAGNRPYTDFDPVNCTNGNCERRA
                     AYLKSATDLLVADLDEMVANWGAEGAARKDVLSKPDAEALATILTGLGSLSYGELAGE
                     RMKLGLILHDPEEEHDCFSDNTHNSHYYDVAGMWSIYSGSVPRRDGTVLEGPGIQDYA
                     ASAAPEAEARLDGLMQDALARFKAIKDKGDSGAMAYDQMIGAGNAEGNALIQAAIDSL
                     VAQTRGIEAMVTALDLKIKVEGSDSLDNPAAVQAP"
     gene            complement(3584..4096)
                     /locus_tag="AC16A0_05310"
     CDS             complement(3584..4096)
                     /locus_tag="AC16A0_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104784.1"
                     /translation="MTANTRDLLARQPERLVISGFRNIMAAYELADADCWEAVWRDFI
                     GALGAPAARRLVGELQFWARCIRLNAEKPLAYFPQCCLNLCHDECMALSIVSAGQARD
                     AETGLRAARYLTGTSDMLRLRDVWHATLPFAAALHDARLAMYPVTVEVVDSISAMNRV
                     AQLQSPRTLN"
BASE COUNT          744 a         1363 c         1454 g          764 t
ORIGIN      
        1 gcggtctgcg cagcatccat gcggcaggcg acgaagaggc gctggcgcag ggcctcgccg
       61 cgtgccgtcg cgctgcccgc aagtcccgcg agcatgagcg tcaggatctg gcggcgatca
      121 atcgccatcg aggctgttga atccgatggt gacgccgagg gctgccggaa tctcgatcgc
      181 cgcacggtcc tcggcggcat cgacggcatc gcgcagcagg atcgcctcga aacgcctgtc
      241 gctgacggcg atcgcaccca ggtcgccgga gaggcctgtg gcaatgcgct gcgcctggcg
      301 cgtggtcgag acgagggccg cgtcttcagg caggcttgag gccagaacgg aattgacgcg
      361 ggccaggcct tccagattat gcgcgatgtt gcggaagctc cggccgctcc gccaggattc
      421 ggccgccctg ggtttcacat cggccggcgt cttgccgatc acggcctcga tcttgctatc
      481 cttgataatg gcgaaactgg tgaccagatc ggtggcaagt gccgtgcgcg actccgcgct
      541 ggcgctcggc gcagctgtgg tccaggcaac ctgcatctgt cctgcaagtt cggccgcatt
      601 ggttgcgatg gcgggtccga gttcgcagcg gtcggccgca tgttcgtcgt tgaagatcag
      661 acgctcaagc gccggcagtc cctgcacagc cgcactggcg gcggaaacct gtgcgggagt
      721 cgcggcagcg gagtcggcac gcaatgctgc aagtccgcgg ctcacggcat tcttgcgttc
      781 cggccagaag tggaagcgtt cgtgccggaa gtcccttgac gagggtccgg acctgaagat
      841 ctcgacttct gcccagcggt cggcggtgtc gttgaaggat gcgacaagtc cttcgcgcaa
      901 agcctcggaa ggcgccgcac agaaggactg ccagtctgcc gcatgccggc tggctgcgct
      961 ggcgagattg ccgaagcccg gcgcgacgac ctctgcgacg atgcgatcgt agaggcgatc
     1021 gggactgtct gctgcctgcg ccggcacaag ggccagcgtc agcacggaga gtcccgcgag
     1081 catcggcttc agcatggttc cgtcctagag ttgcgcgagg aagtctagga gcaactgcct
     1141 ttcccgtcca tcgagctgac ggaagcggtc gcgcgcgcgc gcggcttcgc cgccatgggt
     1201 cagtatggca tcttcgatgg tcgcggcctt gccgccgtga agatagcggc gcgttccctg
     1261 gcgcggatcg agatcgatca gcggggccgt gcgccactct cctggaaggg cactgccgtc
     1321 acgcaatgcg ctgctggtgg catcacccat gtcatgcagc agcaggtcgc tgaagatcgt
     1381 aacaggcatg ccgtggcgat caggcaactg cggcacgtgg cagccgctgc aaccgctgtc
     1441 gcggaagatt tcgaagccgc gcacggccgt gtctggcttg cgcggttcaa gagttgcgac
     1501 ataggccgtg atcagccgga ccatgtcggc ggagatctct tcgttcgccg tgatggcgct
     1561 gcgccccgtc gcatgtgcga ggcagtccgc ctggtccgac gtgcagtcgc catgtggccg
     1621 gggcacaagc ggcgacgaca ggccgaggtc gagcgcagcc gcagcagcgg tctgctgcag
     1681 caggttcgcg cctgttgcct tgctgccgaa gcggcccggg accagtcgtc cgcccccatc
     1741 agcgatcatg ttggcgcggc cggaaagtcc gtcgccgtcc cggtcggacg gatcggcaag
     1801 agcaagaatg gctgtttcgt cgatcctgtc gatcgcgccg cgtccgcgca ggcttggggc
     1861 gatccgcgtc tcaagcacga tcgcatcgtc aggctcctgc agccgggtct cgacaaccag
     1921 agccttgccc cgaaggcgga ctgcgaccct ggcttcggcg gcgagccccg gcaatgccgc
     1981 agtctgaagc tgggcgccca gcagcggatg tgggcgccca tcggaagcgc cgctccgcac
     2041 gaccagtccg cgcgccttga accagccgtt gtccgtctcg aaacgggccg ggccaccgcc
     2101 ggcatggcag gcattgcagg agcgcgcatt gaacagcggg cccaacccat ctgatgcatc
     2161 ggtggacgcc ggggcggcca cccagttgcg ggcaaacagg gccttgccca tggccgtgtc
     2221 gaggggatcg gctgcggccg ttgccgccat ggccagaacg gccacaagac tgacgggaaa
     2281 tcgcctcacg gagcttgcac tgccgcggga ttgtcgaggc tgtccgatcc ctcgaccttg
     2341 atcttgaggt cgagcgccgt gaccatcgcc tcgatgccac gggtctgggc gacgaggctg
     2401 tcgatggcgg cctgaatgag cgcgttacct tcggcattgc cagcgccgat catctggtca
     2461 taggccatgg cgccactgtc gcccttgtcc ttgatggcct tgaacctggc cagcgcatcc
     2521 tgcatcaggc catccagcct ggcctctgcc tcgggtgcag ccgacgccgc atagtcctgg
     2581 atcccggggc cttcgagcac cgtgccgtcg cgccgcggca cgctgccgga gtagatcgac
     2641 cacatgccgg ccacatcgta gtagtgcgaa ttgtgagtgt tgtcggaaaa acagtcatgc
     2701 tcttcctcgg ggtcgtggag aatgagtccc agcttcatcc gctcgccggc cagttcgcca
     2761 taagacagac tgcccagccc cgtgaggatg gttgcgaggg cctccgcgtc gggcttcgag
     2821 agtacgtcct tccgggccgc accctcggcc ccccagtttg caaccatctc atcgagatca
     2881 gccacgagga ggtcggttgc cgacttgagg taggcagcac ggcgctcgca gttgccattg
     2941 gtgcagttga cgggatcgaa gtccgtatac ggacggtttc ccgcgccggg accggtgccg
     3001 ttcaggtcct ggccccagag gaggaactcg atggcgtggt agccgaccga gacatttgcc
     3061 tccgagtcgg ccgcctcgtg cagcgacttg atgagatcgg gtgtaatcgc cgaggcgtcg
     3121 atggtttccg tgccgatggt cagcttcgga ttggcgatga cgttcgccgc atagagaaca
     3181 ttctcgtccg actcctcgcc atatgttgcg atgttgacgt agtcgatcag tccctcatcc
     3241 agcggccagg cattcacgcg gccttcccag tcgtcgacaa tggcattgcc aaaccggaaa
     3301 ccctccgtct gctgatacca gggccgcgac tcccgccagg ccttgcgcgc ggtctccagt
     3361 gtctcctgcg aaggcttggc gaccagcgca tcgacggcct tctgcagatc ggccgccgcg
     3421 gcgcgcgcat cgccatacat ggcctcggcc atgtcggcgt aatgggtggc tacggccttg
     3481 ggtgtcgcgg tgtcaccaag cgccagcgag aaggcggccg agagggtaag cgcagttccg
     3541 gccatcaggc caagccaggg gaatttcatg atcgagctcc ggttcagttc aaggtgcgag
     3601 gggattgcag ctgcgccacg cggttcatgg ccgagatcga gtcgacaact tcaaccgtga
     3661 ccgggtacat ggcgaggcgg gcgtcatgga gcgccgcggc gaagggcaac gtggcatgcc
     3721 acacatccct caggcgaagc atgtcgctgg tgccggtgag atagcgggcg gcacgcagcc
     3781 ccgtttcggc atcacgggcc tgaccggccg agacgatcga cagcgccatg cattcgtcgt
     3841 gacagaggtt caggcagcac tggggaaaat aggcgagcgg cttctccgcg ttcaggcgga
     3901 tgcagcgggc ccagaactgc agttcgccca cgagacgccg ggcggcgggg gcgcccaatg
     3961 ccccgatgaa gtcccgccac accgcctccc agcagtcggc atcggccagt tcgtaggccg
     4021 ccatgatgtt gcggaagccg gaaatgacca ggcgttcagg ctgccgtgcc aggagatccc
     4081 gcgtattggc tgtcatgtct ggttgcgccc cactaatccg agtaatttac tcagatagca
     4141 gtacacgagt gaggcagcaa gaaaaatgtg agcaagatca atatattaga attgttctag
     4201 actggggacg actgcttcag ggggcgatca ggggctcaaa acgcttgtcc gtcagtgtct
     4261 tgaggaaggc aacgagcgcg tcgatttcgc ggtccttgag ggccggaccg aacgcgagtt
     4321 cttcg
//
LOCUS       DBNGVR010000247         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3233290, whole genome shotgun sequence.
ACCESSION   DBNGVR010000247 DBNGVR010000000
VERSION     DBNGVR010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3233290"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="AC16A0_05315"
     CDS             <1..517
                     /locus_tag="AC16A0_05315"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HIR3104785.1"
                     /translation="KRIEAVVHNQDNREHDLLDSDDYYQFEGGMSAAVEEASGQRPRV
                     YHNDHSRPERPVIRTLEEEVGRVMRSRVVNPKWIAGVKRHGYKGAFEIAATVDYMFAF
                     AATTGAVRSHHFDLAYDAFIADAGTRDFIRDNNREALREIAARLAEAAARGLWAPRLN
                     SAYEQLRELAA"
     gene            514..1125
                     /gene="cobO"
                     /locus_tag="AC16A0_05320"
     CDS             514..1125
                     /gene="cobO"
                     /locus_tag="AC16A0_05320"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3104786.1"
                     /translation="MTETDDDNARHHEKMAKKKAARDKIMAGKTIEKGLLIVHTGKGK
                     GKSTAAFGMVFRCIGHGMRAGVIQFVKGAWSTGERVVLEKFPDLVTIKAMGEGFTWET
                     QDRARDIAHSRAAWDEAKKMIADPSYRMVLLDELNIVLRYDYLPLDEVLEVLRNKPAD
                     KHIVVTGRNAKDELIEIADLVTEMEQVKHPFRSGVKAQAGIEF"
     gene            complement(1153..1620)
                     /locus_tag="AC16A0_05325"
     CDS             complement(1153..1620)
                     /locus_tag="AC16A0_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3104787.1"
                     /translation="MSEGSAMIKGFNHTSFTVTDIDRAVRFWTATLGFEARSVSPRAG
                     DWQARVTGVPGAELNVAHLYGYGAHIEFIQYTSGASPAPRISPSMACAAHVCLEVSGI
                     EETWKRLIAAGASAQGEVALVDNGPVRGLKAGYLRDPEGILIELIEVPELPPP"
     gene            complement(1617..2357)
                     /locus_tag="AC16A0_05330"
     CDS             complement(1617..2357)
                     /locus_tag="AC16A0_05330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104788.1"
                     /translation="MTRILITGAGSGIGATTARLLAERGCRVAILDRNGSAASAIAAS
                     LGEEKALGAAGDVTDPAAVDAILRTMIARWGGIDGLVNNAGTWDHGQLLDLSLERWRA
                     VFEVNLMAPIAISNAVVPHLARGSSIVNVSSVLGQVAAPGRGPYCVSKSALITLTKMQ
                     AIEWAEKGIRVNAIAPGYIMNETTRALAATGSFDPSDINRRTPLGRFGEEQEVAEAIA
                     FLLDRHRAAYVTGHVLEVNGGWTAYGFT"
     gene            2579..>3051
                     /locus_tag="AC16A0_05335"
     CDS             2579..>3051
                     /locus_tag="AC16A0_05335"
                     /EC_number="4.99.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HIR3104789.1"
                     /translation="MSEKLGVMLCGHGSRDADAVREFAVLSEHLKRRLPQYPVEYGYL
                     EFATPIIRTGLDKLREQGVSRVLAVPGMLFAAGHAKNDIPSVLNTYAAQNGLRIDYGK
                     ELGIDPKMMRAAADRVQEALDAAGSGVPLHETLLVVVGRGASDPDANSNVSKVTRL"
BASE COUNT          559 a         1014 c          982 g          496 t
ORIGIN      
        1 gaagcgcatc gaggctgtcg tgcacaatca ggacaatcgc gagcacgacc tgctcgactc
       61 cgacgactac taccagttcg agggcggcat gagcgcggcg gtggaggagg cctcgggcca
      121 gcgcccgcgc gtttatcaca acgatcactc gcggcccgag cgtccggtga tccgcacgct
      181 ggaagaggag gtcggccggg tgatgcggtc ccgcgtggtc aatccgaagt ggatcgcggg
      241 cgtgaagcgc catggctaca agggcgcctt cgagatcgcc gccacggtcg actacatgtt
      301 cgccttcgcc gccaccacgg gcgccgtgcg cagccatcat ttcgacctgg cctatgatgc
      361 cttcatcgcg gacgccggga cccgcgactt catccgcgac aacaaccgcg aggcgctgcg
      421 cgagatcgcg gcgcgcctcg ccgaggccgc cgcgcgcgga ctctgggcgc cgcgcctcaa
      481 ttcagcctat gaacagctcc gggagcttgc cgcgtgaccg agaccgatga cgacaacgcc
      541 cgccaccacg agaagatggc caagaagaag gccgcccgcg acaagatcat ggccggcaag
      601 accatcgaga agggcctgct catcgtccac accggcaagg gcaagggcaa gtccaccgct
      661 gccttcggca tggtcttccg ttgcatcggc catggcatgc gcgcaggcgt catccagttc
      721 gtcaagggcg catggtcgac cggcgagcgc gtggtgctgg agaagttccc cgacctggtc
      781 accatcaagg cgatgggcga aggcttcacc tgggaaaccc aggaccgcgc gcgcgacatc
      841 gcccattccc gcgctgcctg ggacgaggcc aagaagatga tcgccgaccc ttcctaccgg
      901 atggtgctgc tcgacgaact taacatcgtc ctgcgttacg attacctgcc gctggacgag
      961 gtgctggagg ttctgaggaa caagcccgcc gacaagcaca tcgtggtgac cggccgcaac
     1021 gccaaggacg agctgatcga gatcgccgac ctggtgaccg agatggaaca ggtgaagcac
     1081 cccttccgct ccggcgtgaa ggcacaggca ggaatcgagt tctgacgtaa aggcggcggg
     1141 agaccttgcg cctcagggtg gcggcagctc cggaacttcg atcagttcga tgagaatgcc
     1201 ttcgggatcg cgcaggtagc ctgccttcag cccacggacc ggcccgttgt cgacgagtgc
     1261 gacttcaccc tgggcgctgg cgcctgccgc gatcagtcgc ttccatgtct cttcgatccc
     1321 ggagacttcc aggcagacat gggcggcaca tgccatgctg gggctgatgc gaggtgccgg
     1381 ggacgcaccg ctggtatact gtatgaactc gatatgggcg ccatagccat agagatgcgc
     1441 cacgttcagc tccgcgccgg gcacacccgt cacccgggcc tgccagtcac ccgcgcgcgg
     1501 cgatacggac cgcgcctcga agccgagcgt tgcggtccag aatctcacgg cgcggtcgat
     1561 gtcggtcacg gtgaagctcg tgtgattgaa acccttgatc atggcgctac cctcgctcac
     1621 gtgaatccat aggcggtcca gccgccgttc acttccagca cgtgcccggt cacataggcc
     1681 gcgcgatgcc gatcgagcag aaaagcaata gcttcggcga cctcctgttc ttcaccaaag
     1741 cgaccaagcg gcgtacgccg gttgatgtcg gacggatcga agctgccggt cgctgcgagg
     1801 gcgcgggtgg tctcgttcat gatgtatccc ggcgcgatgg cattgacgcg gatgcctttc
     1861 tccgcccatt cgatcgcctg catcttggtg agcgtgatga gcgcgctctt cgagacgcaa
     1921 tatgggccac gcccgggcgc cgccacctgg ccgaggacag acgacacgtt gacgatcgaa
     1981 ctaccccgcg ccagatgcgg aacgacggca ttcgaaatgg cgatcggcgc catgagattg
     2041 acttcgaaca cggcccgcca gcgctcgagc gagaggtcca gcaattgtcc gtgatcccag
     2101 gtgccggcat tgttgaccag tccgtcgatg ccgccccagc gcgcgatcat cgttcgcagg
     2161 atggcgtcga ccgccgcagg atcggtaaca tcgcccgcgg cccccagggc cttctcctcg
     2221 cccagcgatg cggcaatggc gcttgccgcg ctgccattgc gatcaaggat ggcgacgcgg
     2281 cacccccgtt ccgcaagcag ccgtgccgtc gtggcgccga ttccgctgcc cgctcccgtg
     2341 atgagaatgc gcgtcatgtg ccgaattctc gctgaagcgc cgcattcgcg caagcccatg
     2401 tcgcttggct cgcttgaccc tgtaacgggt ggcgcctaga ccatctggca gacgcagcgt
     2461 ccccaggacg ggactgaaag cacagtccgg tgcggccgac ccaagcgggg gccaagaccg
     2521 gagctgcctt ccttgccggc ttgccggcgg cgctgcaatg tgtggaaggc aggtcgaaat
     2581 gtccgagaaa ctgggtgtga tgctgtgcgg ccatggcagc cgcgacgccg atgcggtgcg
     2641 cgaattcgcc gtgctctccg agcacctgaa gcgcaggctg ccgcaatacc cggtggaata
     2701 cggttatctc gaattcgcca cgccgatcat ccgcaccggg ctggacaagc tgcgcgagca
     2761 gggcgtcagc cgcgtgctgg cggtgcccgg catgctcttc gccgcgggcc atgccaagaa
     2821 cgacattccc tctgtgctca acacctatgc cgcgcagaac gggctccgca tcgactatgg
     2881 caaggaactg ggcatcgatc ccaagatgat gcgcgccgcc gccgaccggg tgcaagaggc
     2941 gctcgacgcg gccggctccg gcgttccact gcacgagacc ctgctggtgg tggtgggccg
     3001 cggcgcctcc gatcccgacg ccaactccaa cgtgtccaag gtgacgcgcc t
//
LOCUS       DBNGVR010000248         3593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2446950, whole genome shotgun sequence.
ACCESSION   DBNGVR010000248 DBNGVR010000000
VERSION     DBNGVR010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2446950"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2183
                     /locus_tag="AC16A0_05340"
     CDS             <1..2183
                     /locus_tag="AC16A0_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine N-oxide demethylase"
                     /protein_id="HIR3104790.1"
                     /translation="ERYLVRGGGSVTVRVGPGDRLRVVDLEGLQACEIVPRGTDGRAD
                     AAMLGGSPGAKQIRIFGEGPAGEAAAFTVQREGIVKVSAPGGEMDVQRQDTATDLELF
                     ITRAVPARNKSGDQPLPDPLADILQDIRVPAAAAKAYMVKAGEYIQVIDVSGRQCTDF
                     QCFSARKLDRGLENALDATITRTLTGRSYPTPGLPSKMFGRDFEPLVEIVQDTVGRHD
                     AFATACNSRYYDDMGYPGHVNCTENFNRALDPYGIAPRKGWEALNFFYNTNIDAHNQL
                     YLDEPWSRPGDYVLMKALTDLVCVSSSCPDDIDAANAWDPTDIHVRTYSGKETFTRAV
                     AYRMTPDADPQLTRETAFHPRLSALTRNFTEYRGYWLPTRFNNDGAIEEYWACREKCV
                     VLDLSPLRKFEVTGPDAEALLQYCLTRDVRKLAVGQVVYSAMCYENGGMIDDGTLFRL
                     GQNNFRWIGGDDYGGRWLREQAEKKGYKAWVRSSTDQLHNIAVQGPNSRELLKEIIWT
                     SPAQPSIGELEWFRFCVGRIGHFEGAPVVVSRTGYTGELGYEIFCHPKDAVAVFDAVW
                     EAGQKHGIRPMGLDALDMVRIEAGLIFANYEFDSETDPFEAGIGFTVPLKTKADDFIG
                     REALIKRRDHPRRKLVGLEIDSNEAVGHGDCVHIGKAQVGVVTSGMRSPLLGRTIALA
                     RVDALTSEIGTAVEIGKLDGHQKRLPARVVRFAHYDPEKLRPRA"
     gene            complement(2252..>3593)
                     /locus_tag="AC16A0_05345"
     CDS             complement(2252..>3593)
                     /locus_tag="AC16A0_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3104791.1"
                     /translation="TKRVAVIGAGPSGLAQLRAFQSAAQKGAEIPEIVCFEKQDDWGG
                     LWKYTWRTGLDEYGEPVHCSMYRYLWSNGPKEGLEFADYSFEEHFGKPIPSYPPRAVL
                     WDYIVGRVKKAGVRKWIRFNSPVRMVTYDKKKKKFHVTAHDRPKDRMYTEEFDYVICA
                     SGHFSTPNTPFFEGLDSFNGRVLHAHDFRDALEFKGKDVLIVGRSYSAEDIGSQCWKY
                     GAKSVTTTYRSKPMGFKWPKNFEERPLLQKVEGKTAFFKDGSTKEVDAIILCTGYQHY
                     FPFLPEDLRLKTANRLWPANLYKGVVWQDNPRFFYIGMQDQFYTFNMFDAQAWYVRDI
                     ILGRIKLPKPDAMKKDWTKWLKREEKLQDAEQMIWFQGDYVNDLIQATDYPTFDIEGV
                     NKTFMEWEHHKMEDIMGFRNNSYRSLMTGTMSPKHHTPWKDALDDSMEAYLQTK"
BASE COUNT          665 a         1096 c         1147 g          685 t
ORIGIN      
        1 tggagcgcta tctggtgcgc ggcggcggct ccgtgacggt tcgcgtgggt ccgggcgacc
       61 ggcttcgggt cgtcgatctg gaagggttgc aggcctgcga gatcgtgccg agaggaaccg
      121 atggccgagc cgatgcggca atgcttggcg gcagtcccgg tgccaagcag atccgcatct
      181 tcggcgaagg gcctgctggg gaggccgccg cgttcaccgt tcaacgtgag ggcatcgtaa
      241 aggtctccgc gccgggtggc gagatggacg tccaacggca ggacacggcg accgatcttg
      301 aactgttcat cacgcgggcg gttcccgcaa ggaacaagtc aggcgaccag cccttgcctg
      361 accctctggc cgacatcctg caggacatcc gtgttccggc tgccgcagca aaggcctaca
      421 tggtcaaggc cggcgaatac atccaggtca tcgacgtgtc cggccgtcag tgtacggact
      481 tccagtgctt ctcggcacgc aagctcgaca ggggtctgga gaatgcgctg gatgccacca
      541 tcacccggac cttgacgggg cgcagctatc ccacgccagg cctgccctcg aagatgttcg
      601 gccgggattt cgaaccgctg gtcgagatcg tccaggacac ggtcgggcgt cacgacgctt
      661 tcgcaacggc ctgcaacagc cgctactacg acgacatggg ctatcccggc cacgtgaact
      721 gcacggagaa cttcaatcgc gcgctcgatc catacggcat cgcaccgcgc aagggctggg
      781 aagcgctcaa tttcttctac aacaccaata tcgatgccca taaccagctc tatctcgacg
      841 agccgtggtc gcggccgggc gactatgtgc tgatgaaggc gctgaccgat ctcgtctgcg
      901 tctcctcgtc ctgtcccgac gacatcgatg cagccaatgc ctgggacccg accgacattc
      961 atgtccgcac ttattccggc aaggaaacct tcacacgagc ggtggcctat cgcatgaccc
     1021 cagacgccga tcctcagctg acgcgcgaga ccgcgtttca cccgcgcctc tcggcgctga
     1081 cgcgcaactt cacggaatat cgcggctact ggctgccgac ccgcttcaac aacgacggcg
     1141 cgatcgagga atattgggcc tgccgcgaga aatgcgtggt tctggatctg tcgccgctgc
     1201 gcaagttcga ggtgacggga ccggatgccg aggcgctgct gcagtattgc ctgacgcgcg
     1261 acgtgcgcaa gcttgcggtg gggcaggtgg tctattccgc catgtgctac gagaacggcg
     1321 gcatgatcga cgacggcacg ctgttccggc ttggccagaa caacttccgc tggatcggcg
     1381 gcgatgacta tggcggcagg tggctgcgcg aacaggccga gaagaagggc tacaaggcct
     1441 gggtacggtc atcgacggac caactgcaca acattgcggt gcagggtccg aacagccgcg
     1501 aactcctgaa ggagatcatc tggacctcgc cggcgcagcc gtccatcggc gagcttgagt
     1561 ggttccgatt ctgcgtgggg cgcattggcc atttcgaggg cgcgccggtg gtggtgtcgc
     1621 gcaccggtta caccggcgaa ctcggctacg agatcttctg ccacccgaag gacgccgtgg
     1681 cggtcttcga tgcggtatgg gaggcgggac agaagcatgg aatccgaccg atggggctgg
     1741 atgcgctcga catggtgcgg atcgaggcgg ggctcatctt cgcgaactac gagttcgact
     1801 cggagaccga ccctttcgag gcgggcatcg gcttcacggt gccgctcaag accaaggcgg
     1861 acgacttcat cgggcgcgag gcgctgatca agcgccggga ccatccgcga cgcaagcttg
     1921 ttggcctcga gatcgactcg aacgaggcag tgggccacgg cgactgcgtg cacatcggca
     1981 aggcacaggt gggcgtggtg acgagcggca tgcgctcgcc gctcctgggc aggaccatcg
     2041 cgctcgcgcg ggtcgatgca ctcaccagcg agatcggaac ggcggtggag atcggcaagc
     2101 tggacgggca ccagaagcgc ctgccagcgc gcgtcgtgcg cttcgcccac tacgaccctg
     2161 aaaagctgcg cccgcgggcg tgatcggacg gcgatgcgaa acggaaaatg gccgggcaac
     2221 gcccggccat ccgtcttgcc ctacaggatg actacttcgt ctgcagatag gcttccatgg
     2281 agtcgtcgag cgcgtccttc catggcgtgt gatgcttggg cgacatggtg ccggtcatca
     2341 gcgaacggta cgagttgttc cggaacccca tgatgtcttc catcttgtgg tgttcccatt
     2401 ccatgaaggt cttgttcacg ccctcgatgt cgaaggtcgg gtagtcggtg gcctggatca
     2461 ggtcgtttac gtagtcgccc tggaaccaga tcatctgctc ggcgtcctgc agcttctcct
     2521 cgcgcttcag ccacttcgtc cagtccttct tcatggcgtc gggcttgggc agcttgatgc
     2581 ggccaaggat gatgtcgcgc acataccaag cctgggcgtc gaacatgttg aaggtataga
     2641 actggtcctg catgccgatg taaaagaagc gcggattgtc ctgccagaca acgcccttgt
     2701 agagattggc cggccagagc ctgttggcag tcttcaggcg gaggtcttcc ggcaggaacg
     2761 ggaagtagtg ctggtagccg gtgcacagga tgatggcgtc cacctccttg gtgctgccgt
     2821 ccttgaagaa ggcggtcttg ccctcgacct tctgcagcaa gggccgctcc tcgaagttct
     2881 tcggccactt gaagcccatg ggtttggagc gataggtggt ggtgaccgac ttcgcgccat
     2941 acttccagca ttgcgagccg atgtcttccg ccgagtagct gcggccgacg atcagcacgt
     3001 ccttgccctt gaactccagc gcatctcgga agtcgtgggc atgcagcacg cggccattga
     3061 agctgtcgag accctcgaag aagggggtgt tcggggttga gaaatggccg ctggcgcaga
     3121 tgacgtaatc gaactcctcc gtgtacatcc ggtccttggg ccgatcatgc gcggtaacat
     3181 ggaatttttt cttcttcttg tcgtatgtca ccatgcgcac tggcgagttg aagcggatcc
     3241 acttgcgtac gccagccttc ttcacccggc cgacgatgta atcccacagc accgcgcgcg
     3301 gcggatagga aggaatgggc tttccgaaat gctcctcgaa ggaatagtcg gcgaactcca
     3361 gcccctcctt ggggccgttg gaccagagat agcgatacat cgaacagtgg acgggctcgc
     3421 catattcgtc gaggccggtg cgccaggtgt atttccacag gccgccccaa tcgtcctgct
     3481 tttcgaaaca gacgatctcg gggatctcgg cacccttttg cgccgcagac tggaacgccc
     3541 gcaactgtgc cagtcccgac ggtcccgccc cgatcaccgc tacccgtttc gtc
//
LOCUS       DBNGVR010000249         2281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_90575, whole genome shotgun sequence.
ACCESSION   DBNGVR010000249 DBNGVR010000000
VERSION     DBNGVR010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_90575"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1131)
                     /locus_tag="AC16A0_05350"
     CDS             complement(16..1131)
                     /locus_tag="AC16A0_05350"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR3104792.1"
                     /translation="MKIAIPTESHDGERRVAASPDTVKAYVKKGLEVDVQAGAGAGSS
                     ISDDAFAAAGAKIVKEAGKDADIVLTVRRPSAKLLKAMKNDAILMGGLEPYGDKAGLE
                     AIAKAGVTAFAMELMPRITRAQAMDILSSQANLAGYKAVIDAAAHFTRAFPMMMTAAG
                     TVPPAKVFIMGVGVAGLQAIATARRLGAIVSATDVRKATEEQVKSLGAKFVFADVADA
                     ATSGGYAKELSAEDKAKQAALVADHVKGQDIVITTALIPGRPAPVLITKEMVESMKPG
                     SVIVDLAVERGGNCPLSRPDEVVVHNGVSIVGTVNLAGRLAGNASPLFAKNLANFLDL
                     MVQKDGSFKIDEADECIKGTMIAKGGELVHAMFTGAK"
     gene            complement(1149..1439)
                     /locus_tag="AC16A0_05355"
     CDS             complement(1149..1439)
                     /locus_tag="AC16A0_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104793.1"
                     /translation="MANHGSTGSQSHGFADHKSTYDNFIKGSIALTIICLFILVALVA
                     FAFAPSSNMLLGFGGLILGILAVLIDARAGGRWLLSGGLLVIFGLVTAVAVS"
     gene            complement(1627..>2281)
                     /locus_tag="AC16A0_05360"
     CDS             complement(1627..>2281)
                     /locus_tag="AC16A0_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR3104794.1"
                     /translation="TRDVMTLAHELGHGVHQVLAARQGALMASTPLTLAETASVFGEM
                     LTFQALLKQTTDPKKRKALLASKIEDMINTVVRQIAFYTFERKLHTARKSGELTPDQI
                     SAIWLEVQGESLGDSIEIGPGYETFWAYIPHFIHSPFYVYAYAFGDCLVNSLYARYRE
                     SAQGFQDKYFEMLKAGGTKHHSDLLRPFGLNAADPTFWDKGLSVISGMIDELEAMEA"
BASE COUNT          409 a          716 c          738 g          418 t
ORIGIN      
        1 gggcgatctg ttccatcact tcgctcccgt gaacatggca tgcaccagct cgccgccctt
       61 ggcgatcatg gtgcccttga tgcattcgtc ggcctcgtcg atcttgaagc tgccgtcctt
      121 ctggaccatg aggtcgagga agttggcgag gttcttggca aagagcggcg aggcattgcc
      181 cgccagcctg ccggcgagat tgacggtacc gacgatcgac acgccgttgt ggacgacaac
      241 ttcgtcaggc cgcgacagcg ggcagttgcc gccgcgctcg accgcgagat cgacgatcac
      301 cgaaccgggc ttcatcgact cgaccatttc cttggtgatc agcaccgggg ccgggcggcc
      361 cggaatgagt gccgtggtaa tgacgatgtc ctgacccttg acgtgatcgg ccaccagagc
      421 ggcctgcttg gccttgtcct cggccgacag ttccttggca tagcctccgg aggtggcggc
      481 atcggccaca tcggcaaaga cgaacttggc gccgaggctc ttgacctgct cttcggtggc
      541 cttgcgcacg tcggtggccg agacgatggc gccaaggcgg cgtgccgtgg cgatcgcctg
      601 caaccccgcg acgccgacgc ccatgatgaa gaccttggcc ggcggcacgg tgcccgccgc
      661 cgtcatcatc atcggaaagg cgcgggtgaa atgggccgct gcatcgatca cggccttgta
      721 gccggcaagg ttggcctgcg acgacagaat gtccatggcc tgggcgcggg tgatgcgggg
      781 catcagttcc atcgcaaagg cggtgacgcc ggccttggca atggcctcga ggccggcctt
      841 gtcgccatag ggctcgaggc cgcccatgag gatggcatcg ttcttcatcg ccttcagaag
      901 ctttgccgag ggccggcgga cagtcaggac gatatcggcg tccttgcccg cctccttgac
      961 gatcttcgca cccgctgcgg caaaggcatc gtcggaaatg ctggagccgg cgccggcacc
     1021 cgcctgaaca tcgacctcaa ggcccttctt cacataggcc ttgaccgtgt cggggctggc
     1081 cgcgacgcgg cgctccccat catggctttc ggtggggatc gcaattttca tggggttccc
     1141 ttgactgctc aggagacggc tacggcggtg accaggccga agatcaccag cagcccgccc
     1201 gagagcagcc agcggccgcc ggcgcgggca tcgatgagca cggccagaat cccgaggatc
     1261 aggccgccga agccaagcag catgttgctg gatggcgcga aggcgaaggc aaccagcgcc
     1321 accaggatga agaggcagat gattgtcagg gcaatcgagc ccttgatgaa gttgtcataa
     1381 gtacttttat ggtccgcaaa gccatgggat tgcgaaccgg tcgaaccgtg attggccatg
     1441 agtgtccccc gcgagatagg tgtcctttga cctgtttcta gccaagccgc aaggcccctg
     1501 caagacgcac gcaaccacgc ggcgatcggt cactctgctg acgtgaggag tcttgtgccg
     1561 gacccgtcaa gcaactctgt caatcactgc tgacaggcct catgccggcg cggccggcgc
     1621 aaaccatcac gcctccatgg cctccagttc gtcgatcatg ccggagatca ccgacaggcc
     1681 cttgtcccag aaggtaggat cggcggcatt gaggccgaag gggcgcagca ggtcggaatg
     1741 gtgcttggtg ccgccggcct tcagcatctc gaagtacttg tcctggaatc cctgggcgct
     1801 ttcgcggtag cgcgcataga gcgaattcac cagacagtcg ccgaaggcat aggcgtagac
     1861 atagaagggg gagtggatga agtgcgggat ataggcccag aaggtctcgt atcccgggcc
     1921 gatctcgatg ctgtcgccca ggctctcgcc ctgcacctcc agccagatcg cgctgatctg
     1981 gtcgggcgtg agttcgcccg acttgcgcgc cgtgtggagc tttcgctcga aggtgtagaa
     2041 ggcaatctgg cgcacaaccg tgttgatcat gtcctcgatc ttcgaggcca gcagcgcctt
     2101 gcgcttcttg gggtcggtcg tctgcttgag caacgcctgg aaggtcagca tctcgccgaa
     2161 gacgctggcc gtctcggcca gggtcagcgg cgtcgaggcc atcagcgcgc cctggcgcgc
     2221 cgccagcacc tggtgcacgc catgccccag ttcatgcgcc agagtcatga cgtcgcgcgt
     2281 c
//
LOCUS       DBNGVR010000250         3038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5394154, whole genome shotgun sequence.
ACCESSION   DBNGVR010000250 DBNGVR010000000
VERSION     DBNGVR010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5394154"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..2181
                     /gene="clpB"
                     /locus_tag="AC16A0_05365"
                     /pseudo
     CDS             37..2181
                     /gene="clpB"
                     /locus_tag="AC16A0_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
     gene            2242..2604
                     /locus_tag="AC16A0_05370"
     CDS             2242..2604
                     /locus_tag="AC16A0_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR3104795.1"
                     /translation="MTLDDYTSFCAALPHTSHVVQWGGAHVWKVAGKVFAIAGWNDGD
                     ELAVTFKCSAMSFDRLKDKPGMRPAPYLASRGMLWLQRTGPQTLKDKDLKGYIRESHR
                     LVAAGLPKRVQKELGLGG"
     gene            complement(2638..>3038)
                     /locus_tag="AC16A0_05375"
     CDS             complement(2638..>3038)
                     /locus_tag="AC16A0_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3104796.1"
                     /translation="NDGPLVPDLAKELRPGTIVHDKMIFGLAGQLPILDDVRRQGRDE
                     CVLVGLETDVCIAQSAIGLMQEGFRVAVIEDATASPPPHHEAGLRRIAGAGGVITNCK
                     GIHYEWVRDLATLGVVNQKLGTDLPSGLTL"
BASE COUNT          607 a          967 c          994 g          470 t
ORIGIN      
        1 aatcagcggg tcatggacca gaactcctaa gcgaaattcc aggtactgga aaaatacacc
       61 cgcgacctga ctaaactcgc ccgcgacaaa aaactcgatc cggtcatcgg ccgcgatgag
      121 gagatccgcc gcaccatcca ggtgctgtcg cggcgcacca agaacaatcc ggtgctgatc
      181 ggcgaacccg gcgtcggcaa gacggcgatc gccgaaggcc tggccctgcg catcatcaag
      241 ggcgacgtcc ccgagagcct gaaggacaag aagctcctgg cgctcgacat gggtgccctc
      301 atcgcgggcg caaaatatcg cggcgagttc gaggagcgcc tgaagtcggt gttgtcggag
      361 gtgacctccg cggatggcgg catcattctc ttcatcgacg aaatgcatac gctggtcggc
      421 gccggaaagg ccgatggcgc gatggacgcg tcgaacctgc tgaagccggc tctggcgcgc
      481 ggcgaactgc actgcgtcgg cgccaccacg ctcgacgagt accgcaagca tgtcgaaaag
      541 gacgcagccc ttgcccgccg cttccagccg gtcttcgtga ccgagcccac ggtggaggac
      601 tcgatctcca tcctgcgcgg catcaaggag aagtacgagc tgcatcacgg cgtgcggatc
      661 accgactcgg cgatcgtcgc ggcggcgacc ctgtcgaacc gctacatcac cgaccgcttc
      721 ctgccggaca aggccatcga cctgatggac gaggccgcgg cgcgcctgcg catgcaggtc
      781 gattccaagc cggaagagct cgacgagctg gatcggaaga tcatgcagct gcagatcgag
      841 cgggaagcgc tgaagaagga aaccgacacc gcatccaagg accgtctcga gaagcttcag
      901 aaggaactgg cggagctcga ggagaagtca gcgaccttca cccgccggtg gcaggaggag
      961 aaggaaaagc tcgcctcggc ctccaaggtc aaggaagagc tcgagaaggc ccggaaccag
     1021 ctggaccagg cgcagcgcaa gggcgatttc gccaaggcgg gcgaactggc ctattcgacc
     1081 attcccggtc tcgagaagca gctgaaggac gccgaagccc agagcgcctc gaagatgctc
     1141 gaggaagccg tgaccgaaag ccatgtggcc caggtcgtgt cgcgctggac cggcgttccg
     1201 gtcgacaaga tgctggaagg cgagcgcgag aagcttctgc agatggagaa ggatctcggc
     1261 aagcgcgtcg tgggccagga agaggccgtg gtcgcggtgg ccactgcggt caggcgttcg
     1321 cgcgccggac tgcaggatcc gaaccgcccc atgggatcct tcatgttcct cgggcccacc
     1381 ggcgtgggca agacggaact caccaaggcg ctggccggct tcctgttcga cgacgagcac
     1441 gcgatggtgc gcatcgatat gtcggaatac atggagaagc actccgtctc gcggctgatc
     1501 ggcgcgcctc cgggctatgt cggctacgat gaggggggcg ctctcaccga agcggtgcgc
     1561 cggaggccct accaggtcgt gctcttcgac gagatcgaga aggcgcatcc ggacgtgttc
     1621 aatgtgctgc tccaggtgct ggacgacggc cgcctgaccg acggccaggg ccgcaccgtg
     1681 gacttcaaga acacgctgat catcatgacg tcgaacctgg gcagcgagta tctcgtgaac
     1741 ctcggcgaga cgcaggacgt ggacatggtt cgcgaccagg ttatggcggt ggtcaagggt
     1801 cacttccggc ccgaattcct gaaccggctc gacgagatca tcttgttcca caggctgaag
     1861 cgcgagcaca tgggttccat cgtcgacatc cagctgggcc ggctgcagaa gctgctggcc
     1921 gaccgcaaga tcaccatcga ggtgaacgag gcggcgcgca gctggctggc cgacaagggc
     1981 tacgacccgg cttatggcgc gcgcccgctg aagcgcgtga tccagaagaa cgtacaggac
     2041 ccgctggccg aggaacttct cgccggacgg atcaaggacg gcgacacggt gaagctcgac
     2101 gccgtcgccg gtacgctcac cttcaacggc ctcgcggtcg gcggcaagcc cgtcaatccc
     2161 aaggtcgtga gcctgcactg aacctgctag actcggacgc gccggagacc ttctccggcg
     2221 cgtttccact tgcggcagac catgaccctc gacgactaca ccagcttctg cgccgccctg
     2281 ccccacacga gccatgtggt gcaatggggc ggcgcccatg tctggaaggt ggcgggcaag
     2341 gtctttgcca ttgccggatg gaacgatggt gacgaacttg ccgtcacctt caagtgttcg
     2401 gccatgagct tcgatcggct caaggacaag ccgggcatgc ggcccgcgcc ctatctcgcc
     2461 tcgcgcggca tgctgtggct gcagcgaacc ggcccgcaga cgctgaagga caaggacctc
     2521 aagggctaca tccgcgagag ccaccgcctg gtggccgccg gcctacccaa gcgcgtgcag
     2581 aaggaacttg gcctgggcgg gtaagggctt gccgtgccgg tccttctccc gttccgacta
     2641 caacgtcagg cccgacggca ggtcggttcc cagcttctga ttcaccacgc cgagcgtcgc
     2701 gagatcgcgg acccattcgt agtggatgcc cttgcagttt gtgatcacgc cgccggcgcc
     2761 ggcgatgcgg cgcagcccgg cctcatggtg gggcggcggc gaagctgtgg catcctcgat
     2821 cacggccacg cggaagccct cctgcatcaa accgatggcc gactgcgcga tgcagacatc
     2881 cgtttcgagt ccgacgagaa cgcattcgtc gcggccttgc cgccgcacgt cgtcgagaat
     2941 cggcagctgg ccggcaaggc cgaagatcat cttgtcgtgg acaatggtgc cgggcctcag
     3001 ctccttcgcg agatccggca ccagcggccc atcattgg
//
LOCUS       DBNGVR010000251         4579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3627887, whole genome shotgun sequence.
ACCESSION   DBNGVR010000251 DBNGVR010000000
VERSION     DBNGVR010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3627887"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /locus_tag="AC16A0_05380"
     CDS             complement(<1..1979)
                     /locus_tag="AC16A0_05380"
                     /inference="COORDINATES: protein motif:HMM:NF021606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HIR3104797.1"
                     /translation="MDGFSVLIAIAAFVLGLVALHKMSRLETLVAQLRMQIDQLLAEA
                     GRSGMADLRETAAAAGPDLAPSAPIAAEAVTEPPAVEAPPDLAVVKALQPSAPPPVRD
                     MEQTLASRWFVWIGGVAIAIGGLLFVKYAYDNGLIPPTLQIILGVLAGAVLVAAGELV
                     RRKGTSRLRTADDYVPAALSAAGLAILFASIFAAYALYEVIGGFPAFIGLAGVALGAL
                     ALSRWQGPFIAALGLLGSYVTPALIPSAEPSAWGFFAYLLIILAASFAVLRGRNWWWL
                     GYLALAGAGAWGLLWIKGGIFVADHVWPVGLFALALGIVGVFGLKGRAILDDDSGHLT
                     GPGSMSQPLVIALASLAVAVLLLAILVEQTRHAQPALMLMAAAVLAMTLLAWFSAGLS
                     PLAPIAGLLMLIVLMAWPEVAFHTLAFDERGFWSSVPGPGAGHFLSWMLAGAVAAALV
                     GVAGNLRRSVPLAWAVLAAGGAFFFTWGAWARVDILLSDTAWSLSALALAAILLAGAW
                     LSRDALAGQPRNTAAGVLAAGAAALLVFALDRHLDGVWLTLAIALLAATYAAAARSIT
                     AQLMGGIAAALGSLTALRLFLSHELWSDDRNLPMGQHWVIYGYGIPVILFLLSSRWLR
                     AAGHARSAVALEGISLGLAISLVSLEIRVLIGGGYL"
     gene            2050..2733
                     /gene="lipB"
                     /locus_tag="AC16A0_05385"
     CDS             2050..2733
                     /gene="lipB"
                     /locus_tag="AC16A0_05385"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3104798.1"
                     /translation="MVSRDEITRSLHSPAPVDWYVSGGLVPYPDALSGMDEVVAGIAQ
                     GEVRERVWLLEHPPLYTAGTSANPADLVSPERFPVFATGRGGQYTYHGPGQRVAYVML
                     DLKRRGGDLRAYVAGLEQWIIATLAAFGIAGERREDRVGVWVRRGDREDKIAAIGIRV
                     RHWVSFHGISINLAPDLSHFDGIVPCGVREHGVTSLRDLGLNVSMAELDAALRRGFEA
                     GFGPTVTAS"
     gene            complement(2720..3121)
                     /locus_tag="AC16A0_05390"
     CDS             complement(2720..3121)
                     /locus_tag="AC16A0_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104799.1"
                     /translation="MRKLKTAGAALALAILAAIPARAGYIDNYRSWMMLGPDGQSAYA
                     MALFDTMSVFIAGDEFSTARAIGLRNCAKALNLKAGMMAQAITKYYQDYPENQVASPF
                     VAFNGYLERGACSPFINDVRREMGLPEVKKQ"
     gene            3260..3871
                     /gene="gstA"
                     /locus_tag="AC16A0_05395"
     CDS             3260..3871
                     /gene="gstA"
                     /locus_tag="AC16A0_05395"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HIR3104800.1"
                     /translation="MKLYYSPGACSLAPHIVLNEIGEPSEIEKVDLGSKKTEKGDNFH
                     DVNSKGYVPTLELAKGEVLTEVSTILQYLADKAEKAKLLPKFGSMERYRAMESLNFVA
                     AELHKGIGGLFNKAMPEDGKKAIAERVSQRLGWLDAQLAKKPYLLGSDYSVADAYAFT
                     VINWCQWVGIDTKQWPHIQAFMQRVGSRPAVQAALKKEGLIPA"
     gene            complement(3907..4185)
                     /locus_tag="AC16A0_05400"
     CDS             complement(3907..4185)
                     /locus_tag="AC16A0_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3104801.1"
                     /translation="MTAVRVIVEGRVQGVGYRAWVSRQAKSRNLNGWVRNRGDGSVEA
                     VFAGDQVTVAAMVEACRSGPGLAVVSDVRQELWPDQPPPGFSVRPSQS"
     gene            complement(4182..>4579)
                     /locus_tag="AC16A0_05405"
     CDS             complement(4182..>4579)
                     /locus_tag="AC16A0_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3104802.1"
                     /translation="WVPGTPVFSADLGQGPVTVQVRRIPNGYKLSYRGVVLETHVYTA
                     REAELARLMPEKAVADTSRKLMCPMPGLVISIAVSEGQDVKAGEALAIVEAMKMENIL
                     RAERDVTVKKLHAKKGDSLAVDAVIMEFV"
BASE COUNT          938 a         1508 c         1412 g          721 t
ORIGIN      
        1 agatagccac cgccgatgag cacccggatc tcgagcgaaa ccagcgaaat ggcaagaccc
       61 aggctgattc cctcaagcgc cacggcggat ctggcgtgac ccgcagcacg cagccaccgg
      121 ctggacaaaa ggaagagaat aaccggaatg ccatagccat agatgaccca gtgctggccc
      181 atcggcaggt tgcgatcatc tgaccacaat tcgtgcgaga ggaacaggcg cagtgcggtc
      241 aagctgccca gcgccgcggc aattcctccc atcagctgcg ccgtgatcga tcgggccgca
      301 gcggcatagg ttgcagcgag cagagcaatg gccagcgtca gccagacgcc atcgaggtgg
      361 cggtcaaggg cgaagaccag cagcgctgct gcacctgcag cgagcacgcc cgccgcggtg
      421 ttgcgcggct gacccgccag ggcatcgcgc gacagccaag caccagccag cagaatggcc
      481 gcgagtgcca gcgctgaaag gctccaggcc gtgtccgaca gcaggatgtc gacccgcgcc
      541 caggcacccc aggtgaagaa gaatgcgcca cctgccgcaa gaacagccca ggcgagaggc
      601 acgcttcgcc gcaggtttcc cgcaacgccc accagggcgg cggcaacggc cccagcaagc
      661 atccaggaca ggaagtggcc tgcaccggga cccggcacgc ttgaccagaa cccgcgctca
      721 tcgaaggcca gtgtgtggaa ggccacttcc ggccacgcca tcaacacgat gagcatcagc
      781 agcccggcga tcggagcgag cggcgaaagg cctgcactga accaggcgag cagagtcatg
      841 gcaagcacag ctgcagccat gagcatgagc gcgggttgcg catggcgcgt ctgttcgacc
      901 aggatggcaa gcagaagcac tgccacggca agacttgcaa gcgcaatgac cagcggctgg
      961 ctcatgcttc cgggtccggt cagatggccg ctgtcatcat ccaggattgc gcggcccttg
     1021 aggccgaaga ctccgacgat tccaagtgcc agcgcaaaga gcccgaccgg ccagacatgg
     1081 tctgccacga agatgccgcc cttgatccag agcagccccc aggcgcccgc acccgccagg
     1141 gccagatagc ccagccacca ccagttccgc ccgcgcagca ccgcgaagct tgcagcaaga
     1201 atgatcagga gataggcaaa gaaaccccac gcactgggtt cggcagatgg aatgagcgcc
     1261 ggggtcacgt aagagccgag cagtccgagc gccgcgatga agggtccctg ccagcgcgag
     1321 agcgcgagcg cgccaagggc gacgcccgcc agaccgatga aggccggaaa cccgccgatc
     1381 acctcataga gcgcataggc cgcaaaaatg ctggcaaaca gaattgccag gccggcggcc
     1441 gaaagcgcgg caggcacgta gtcgtccgcg gtccgcaacc tgcttgtgcc cttgcggcgt
     1501 accagctcgc cggccgcgac cagcacggcg ccggccagaa ctccgagaat gatctgaagc
     1561 gtcgggggaa tcagtccgtt gtcataggcg tacttgacga agaggaggcc gccgatggcg
     1621 atggcaacgc cgccgatcca gacgaaccag cgcgaggcca atgtctgttc catgtcgcgc
     1681 accggcggtg gagcggatgg ctgcaatgct ttgaccacgg cgagatcagg cggtgcctcg
     1741 actgccggcg gctcggtcac ggcttcggct gcaatcggcg ctgacggcgc aaggtcaggg
     1801 ccagcagccg cggcagtctc gcgcaggtcc gccatgccgc tgcggccagc ttcggccaga
     1861 agctggtcga tctgcatgcg cagctgggcg acaagggttt ccagccgcga catcttgtgc
     1921 aatgccacca gacccagcac gaaagcggca atcgcgatca gaacactgaa cccgtccatt
     1981 ccgtactccc ggcccccgac tcaaggcctg cagcttgcca cgaaacagcg aaactgggaa
     2041 aagccgctca tggtctcccg cgatgagata acccgtagcc ttcattctcc ggctccagtc
     2101 gactggtacg tctcgggcgg cctcgtgcca tacccggatg cactctccgg tatggacgaa
     2161 gtggtggcgg gaatcgcgca gggcgaggtt cgcgagcgcg tatggcttct cgagcatccg
     2221 ccgctctaca cggccggcac gtcggccaat cctgcggatc tggtttcacc ggagcgcttc
     2281 cctgtcttcg caaccggacg ggggggccag tatacctacc acggtccagg gcagcgcgtt
     2341 gcctatgtga tgctcgacct gaaacgccgc ggtggcgacc ttcgtgccta tgttgccggg
     2401 ttggaacagt ggatcatcgc aacactggcg gccttcggta ttgccggaga gcggcgcgaa
     2461 gaccgggttg gcgtgtgggt caggcgcgga gaccgggaag acaagattgc tgccatcgga
     2521 atccgcgtcc ggcactgggt gagcttccac ggcatcagca tcaatctggc gcccgatctc
     2581 agccatttcg acggcatcgt cccctgtggc gtacgcgaac acggtgtcac cagcttgcgg
     2641 gacctgggac tgaacgtcag catggccgaa ctcgatgctg cgctgcgccg cggcttcgag
     2701 gccgggttcg ggccgaccgt tactgcttct tgacctccgg cagccccatc tcgcggcgca
     2761 cgtcgttgat gaaggggctg caggcgccgc gctccagata gccgttgaac gcaacaaagg
     2821 gcgaggccac ctggttctcg ggatagtcct ggtaatactt cgtgatagcc tgcgccatca
     2881 tgccggcctt gagattgagc gccttggcgc agttgcgcag gccaatggca cgcgccgtcg
     2941 agaactcgtc gccggcgatg aagacgctca tggtatcgaa cagtgccatg gcataggccg
     3001 attgcccatc gggtcccagc atcatccagg accgatagtt gtcgatgtat cccgctcggg
     3061 ccgggatggc ggccaatatg gcgagggcga gtgcggctcc tgccgtcttg agcttacgca
     3121 tgcaaaatcc ttcgaaatcg tgccgtcact atcctatggc acggaagcgc tgtccaccgc
     3181 gtcacgtccc tacgaaataa ttgtgacttg aattgttacg ataaagtgct acatcggtcc
     3241 cgcacaacgg agaaccacca tgaaactcta ctattccccc ggcgcctgtt cccttgcacc
     3301 gcatattgtc ctcaacgaga tcggcgaacc ctctgaaatc gagaaggtcg acctcggcag
     3361 caaaaagacc gagaagggcg acaacttcca cgacgtgaac agcaagggct acgtgcccac
     3421 gctggaactg gcgaagggcg aggtcctgac cgaagtctcg accatcctgc aataccttgc
     3481 cgacaaggcc gagaaggcaa agctgctgcc caagttcggc agcatggaac gttaccgcgc
     3541 catggaaagc ctgaacttcg tcgccgcgga actgcacaag ggcatcggcg gacttttcaa
     3601 caaggccatg ccggaagacg ggaagaaggc gatcgcagag cgcgtgtccc agcggctggg
     3661 ctggctcgac gcacagcttg ccaagaagcc ctatctgctc ggcagcgact attcggtggc
     3721 agacgcctat gccttcacgg tcatcaactg gtgccagtgg gtcggcatcg acaccaagca
     3781 gtggccccac attcaggcct tcatgcagcg ggtcggctca cgtcccgccg tgcaggcggc
     3841 cctgaagaag gaaggcctga tcccagcatg acaatcgtcg cggagatggg cttcgcccgt
     3901 ctccgctcac gattgcgatg gccgcacgga gaacccgggc ggtggctggt ccggccagag
     3961 ttcctgcctg acgtcgctga ccaccgcgag gccggggcct gaccggcagg cctccaccat
     4021 tgcggcaact gtcacctgat caccggcgaa caccgcctcc accgaaccgt caccacggtt
     4081 tctgacccag ccgttcaggt tccggctttt cgcctggcgg ctgacccagg cccgatatcc
     4141 gacgccctgg acgcgtcctt cgacgatcac ccggaccgcc gtcacacgaa ctccatgatc
     4201 accgcgtcga cggcgagact gtcacccttc ttggcatgga gcttcttcac cgtcacatcg
     4261 cgttcggcgc gcaggatgtt ctccatcttc atggcctcga caatcgccag cgcctccccc
     4321 gccttgacgt cctggccttc gctgaccgca atggaaatga ccaggccagg catggggcac
     4381 atgagtttcc ttgatgtgtc cgccacggcc ttctcgggca tgagccttgc cagttcggcc
     4441 tcccgcgccg tgtagacatg ggtctccaat actacgccgc gatagctcaa cttgtagccg
     4501 ttggggatgc gcctcacctg caccgtcacc gggccttgcc cgagatcggc cgagaagacc
     4561 ggcgtacccg ggacccacc
//
LOCUS       DBNGVR010000252         2786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3038266, whole genome shotgun sequence.
ACCESSION   DBNGVR010000252 DBNGVR010000000
VERSION     DBNGVR010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3038266"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="AC16A0_05410"
     CDS             <1..492
                     /locus_tag="AC16A0_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3104803.1"
                     /translation="DLGYGPARGAEMLSMMLGFGVISRLASGFIADRIGGLATLIAGS
                     FLQMLALILYIPFDGLVSLYVVSALFGLSQGGIVPSYALIIRDHFPAREAGARISMVL
                     TATVFGMALGGWLSGEIYDWTGSYQAAFLNGIAWNVLNLGIALWILFIRSPGKIAPRA
                     ATA"
     gene            640..>2786
                     /gene="ppdK"
                     /locus_tag="AC16A0_05415"
     CDS             640..>2786
                     /gene="ppdK"
                     /locus_tag="AC16A0_05415"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR3104804.1"
                     /translation="MAAESHKFVYAFGGGSAEGRADMKALLGGKGANLAEMANLGLPV
                     PPGFTITTEVCTVFSATGGKYPDGLAAQVDAALAAVGRLVGKNFGDAANPLLVSIRSG
                     ARASMPGMMDTVLNLGLNDRTVEGLAARSGDRRFAFDSYRRFISMYSDVVLGVDHADF
                     EEILGGFKDERGYALDTEITATEWEGLIARYKGLVEESTGQAFPQDVGEQLWGAIGAV
                     FKSWDGARAVTYRKLNAIPQDWGTAVNVQAMVFGNMGETSATGVAFTRNPSTGTRELY
                     GEFLVNAQGEDVVAGIRTPQNITEQARIEANSDAPSLEALMPAVFAELRAHFDRLEQH
                     YRDMQDMEFTIQEGKLWMLQTRIGKRTAKAALKIAVDMAEEGLISKADAVRRIDPSSL
                     DQLLHPTLDPGAKREVIATGLPASPGAASGEIVFDADEAERAKALGRKVILVRLETSP
                     EDIHGMHAAEGILTARGGMTSHAAVVARGMGKPCVSGAGGIRIDHVAGTLSSAGITMR
                     KGEILTIDGSTGQVIRGEVPTIKPALSGDFATLMSWADGLRRMKVRANAETPLDARTA
                     RDFGAEGIGLCRTEHMFFESSRILAVREMILADKLDARKAALAKLLPMQRQDFIEIFE
                     IMAGLPVTIRLLDPPLHEFLPHADHEIEEVADEMNANPDILRSRVAELDEFNPMLGHR
                     GVRLLITYPEIAEMQTRAIFEAATEVARKSGKAP"
BASE COUNT          466 a          928 c          926 g          466 t
ORIGIN      
        1 gacctcggct acgggcccgc gcgcggcgcc gagatgctgt cgatgatgct gggcttcggg
       61 gtcatcagcc gactggcctc gggcttcatc gccgaccgga tcggcggtct cgccacgctg
      121 atcgccggct ccttcctgca gatgctggcg ctcatcctct acattccctt cgacgggcta
      181 gtgtcgctct acgtggtctc agcgctcttc ggcctgtcgc agggcggcat cgtgccgagc
      241 tatgcgctga tcatccgcga ccatttcccg gcgcgcgagg cgggtgcgcg gatctcgatg
      301 gtgctgaccg ccactgtctt cggcatggcg ctgggcggct ggctctcggg cgagatctat
      361 gactggaccg ggtcctacca ggccgccttc ctgaacggca ttgcctggaa cgtattgaac
      421 ctcggcatcg cgctctggat cctcttcatc cgttcgccgg gaaagatcgc gccccgggcg
      481 gcgacggctt gagattgctt gtctgaaacc gtttgcatgg atcggtgtca cgcatgtgcg
      541 atattgcgtt gcactcggtc ggcaagtgtg gtttgaatcg agcagggccg ccgcccgcta
      601 agacttgaaa ctttccaatc cagaatcgag agtgcgacca tggcggccga gtcccacaaa
      661 ttcgtctatg cgttcggcgg cggctcagcc gaagggcgtg ccgacatgaa ggccctgctc
      721 ggcggcaagg gcgccaacct ggccgaaatg gccaatctcg gcctgccggt tccgcccggc
      781 ttcaccatca ccacggaagt ctgcaccgtc ttctcggcaa ccggcggcaa gtatcccgac
      841 ggcctcgcgg cccaggtcga cgcggcgctg gccgccgtgg gccgcctggt gggcaagaat
      901 ttcggcgatg ccgccaatcc gctgctggtg tcgatccggt ccggcgcgcg cgcctcgatg
      961 cccggcatga tggacacggt tctcaatctg ggcctcaacg accggacggt ggaggggctg
     1021 gcggcgcgat cgggtgaccg gcgcttcgcc ttcgacagct accgccgctt catcagcatg
     1081 tattccgacg tggtgctggg cgtcgaccat gccgacttcg aggagatcct cggcggcttc
     1141 aaggacgagc gcggctatgc gctggatacc gagatcaccg ccaccgaatg ggaaggcctg
     1201 atcgcgcgct acaagggcct ggtggaggaa tccaccggac aagccttccc gcaggatgtg
     1261 ggcgagcagc tgtggggcgc catcggcgcg gtgttcaagt cctgggacgg ggcgcgggcg
     1321 gtgacctatc gcaagctcaa tgccattccg caggactggg gcacggcggt caatgtccag
     1381 gccatggtct tcggcaacat gggcgagacg agcgccaccg gcgtggcctt cacccgcaat
     1441 ccctcgaccg gcacgcgcga gctctatggc gagttcctgg tgaacgccca gggcgaggac
     1501 gtggtggccg gcatccgcac gccgcagaac atcaccgaac aggcgcgcat cgaggccaat
     1561 tcggatgcgc cctcgctgga agcgctgatg ccggcggtct tcgccgagct gcgcgcccat
     1621 ttcgaccggc tcgagcagca ttatcgcgac atgcaggaca tggaattcac catccaggag
     1681 ggcaagctct ggatgctgca gacgcgcatc ggcaagcgca ccgccaaggc ggcgctgaag
     1741 atcgccgtcg acatggcgga ggaagggctg atctccaagg ccgacgcggt gcgccgtatc
     1801 gatccatcgt cgcttgacca gctgcttcat cccaccctcg atcccggcgc caagcgcgag
     1861 gtgatcgcca ccggtcttcc ggcctcgccg ggtgcggcct ccggcgagat cgtcttcgac
     1921 gccgacgagg ccgagcgcgc caaggcgctg ggccgcaagg tgatcctggt gcgcctcgag
     1981 accagccccg aagacatcca cggcatgcat gcggccgaag gcatcctcac cgcccgcggc
     2041 ggcatgacct cgcatgcggc cgtggtggcc cgcggcatgg gcaagccctg cgtctcgggc
     2101 gccggcggca tccgcatcga tcatgtcgca ggcacgctga gctcggctgg catcacgatg
     2161 aggaagggcg agatcctgac catcgacggt tcgaccgggc aggtgatccg cggcgaggtg
     2221 ccgaccatca agccggcgct gtcgggcgat ttcgccaccc tgatgtcatg ggccgacggc
     2281 ctgcgccgga tgaaggtgag ggccaatgcc gagacgccgc tggacgcccg cacggcgcgg
     2341 gacttcggtg cggaaggcat cggcctgtgc cggaccgagc acatgttctt cgagtccagc
     2401 cgcatcctcg ccgtgcgcga gatgatcctc gccgacaagc tggacgcccg caaggccgcc
     2461 ttggccaagc tcctgcccat gcagcgccag gacttcatcg agatcttcga gatcatggcc
     2521 ggcctgccgg tgaccatccg cctgctcgac ccgccgctgc acgaattcct gccgcatgcc
     2581 gaccacgaga tcgaggaagt ggcggacgag atgaacgcca atcccgatat cctgcgcagc
     2641 cgggtggcgg aactggacga attcaatccc atgctcggcc accgcggcgt acggctgctg
     2701 atcacctacc cggaaattgc cgagatgcag acgcgggcca tcttcgaggc agcgaccgaa
     2761 gtggcccgga agtccggaaa ggcacc
//
LOCUS       DBNGVR010000253         2893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1663675, whole genome shotgun sequence.
ACCESSION   DBNGVR010000253 DBNGVR010000000
VERSION     DBNGVR010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1663675"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="AC16A0_05420"
     CDS             <1..1047
                     /locus_tag="AC16A0_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104805.1"
                     /translation="PGRAALTADGIALRSVELRGARIDGETRMTAARVDGDLDLTGAR
                     LSHPDGEALHLNRTVVRGGLFLRGGAQIKGALDLTGASVDTLHDDEASWPAPGDLLLN
                     RCLYNALIGGPMDAERRIAWLARQTPDRWGEEFWPQPYEQLAYVFRDMGHDDDAQTVL
                     VEKERLQRAARRARASSPLWRLLLTVKDSLLGVTLGYGRKPLLAFAWLLFFWGLGVAV
                     FAVAQSQSAIKPNSPVILRSPEWILCGVPKDESVTLTAGPAIGRAIPGQSQLHCFLAQ
                     PEASSYPVFNAWMYSLDTLFPVLEIGQKDYWRPDPSRRLGSGIMGYFYFQAIVGWALS
                     LLAIAGFSGLVKSR"
     gene            complement(1063..2115)
                     /locus_tag="AC16A0_05425"
     CDS             complement(1063..2115)
                     /locus_tag="AC16A0_05425"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3104806.1"
                     /translation="MNGSPSAKRSGQSRPDVLIDLALQGGGSHGAFTWGVLDRLLEES
                     WLKIDAVSGTSAGAMNAAVLASGWLQGGAEGARQALEAYWRRVSEAAAFSPFQRTPLD
                     RLMGRWSLDHSPGYVAMDLMTRLASPYDLNPLGYNPLKPILEESIDFEILRQSPIKLF
                     ITATNVRTGRGRIFRNPEISADVLLASACLPTMFQAVEIDGEAYWDGGYAGNPTITPL
                     VRESSASDTILVQINPRERADVPRSAAEILNRLNEISFNASLMKEIRMIALLRQVADP
                     GSGEGARWAGMRTHRIMTELLSELGASSKLNAEWDFVSLLRDEGRRAAAAFLDQHGAD
                     IGARSTADIDVLLSEC"
     gene            complement(2242..2318)
                     /locus_tag="AC16A0_05430"
     tRNA            complement(2242..2318)
                     /locus_tag="AC16A0_05430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2282..2284),aa:Arg,seq:ccg)
     gene            2530..>2893
                     /locus_tag="AC16A0_05435"
     CDS             2530..>2893
                     /locus_tag="AC16A0_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3104807.1"
                     /translation="MSAAAASSRIDWVDYAKGLCIILVVMMHSTLGVEKATGQLSWMH
                     EVVEWARPFRMPDFFLISGLFLASRIDRPWRSYLDSKVLHFAYFYALWVTIQFAAKGY
                     GMTQELGAAGALQAYLMSF"
BASE COUNT          477 a          956 c          949 g          511 t
ORIGIN      
        1 ccggggcgcg ctgcgctcac ggcagacggc atcgcgcttc gttcggtgga actgcgcggt
       61 gcgcggatcg acggcgagac ccgcatgacc gcggcgcgcg tcgacggcga cctcgatctc
      121 accggggcaa ggctctctca tcccgatggc gaggcgctgc acctcaatcg tacggtggtg
      181 cgcggcggac tgtttctgcg cggcggcgcg cagatcaagg gagcgctcga tctcaccggc
      241 gcgtccgtcg acaccctgca cgacgacgag gcatcctggc ctgcgccggg cgatctcctt
      301 ctcaaccgct gcctctacaa cgcgctcatc ggcggcccga tggatgccga acggcgcatc
      361 gcctggctgg cgcgccagac gcccgaccgc tggggcgagg agttctggcc gcagccctat
      421 gaacagctcg cctatgtctt ccgcgacatg ggacatgacg acgacgcgca aacggttctt
      481 gtcgagaagg agcgcctcca gcgcgcagcc cggcgggccc gcgcctccag tccgctgtgg
      541 cggctgttgc tgaccgtcaa ggacagcctg ctcggcgtga cgctcggcta tggccgcaag
      601 ccgcttctgg catttgcctg gctgctcttc ttctgggggc tgggggtcgc cgtctttgcg
      661 gtggcgcagt cacagtccgc catcaagccg aacagcccgg tgatcctacg ctcgccggaa
      721 tggatcctgt gcggcgtgcc gaaggacgag agcgtgacgc tcacggcagg gccggcgatc
      781 ggccgcgcca taccagggca gtctcagctc cactgcttcc tcgcccagcc ggaggcatcg
      841 agctatcccg tcttcaatgc ctggatgtat tcgctcgaca cgctgtttcc cgtcctcgaa
      901 atcggacaga aggactactg gcgtcccgac ccgtcgcggc gcctgggctc gggcatcatg
      961 gggtacttct atttccaggc catcgtggga tgggccttga gcctgctggc gatcgcggga
     1021 ttctcggggc tggtgaagtc gcgctgagga gcccgcccgg gctcagcatt ccgacagaag
     1081 cacgtcgata tcggcggtcg aacgcgcacc gatgtcggcg ccatgctgat cgaggaatgc
     1141 cgccgcggcc ctgcgtccct cgtcgcgcag cagagagacg aagtcccatt ccgcattgag
     1201 cttcgaggat gcgccaagtt ccgagagcaa ttcggtcatg atgcgatgcg tgcgcatgcc
     1261 ggcccagcgg gcgccttctc ccgatccggg atccgccacc tgccggagca gggcgatcat
     1321 gcggatttcc ttcatgagcg acgcattgaa gctgatctcg ttcaggcggt tcaggatctc
     1381 tgcggcgctg cgcggcacgt cggcccgctc gcgcgggttg atctggacca ggatcgtgtc
     1441 gctggcactg ctctcgcgca ccagcggcgt gatggtggga tttcccgcat aaccgccgtc
     1501 ccaataggcc tcgccatcga tctccacggc ctggaacatg gtgggcaggc aggccgaggc
     1561 caggaggaca tccgcactga tctccggatt gcggaagatg cgcccgcggc ccgtccgcac
     1621 attggtggcc gtgatgaaga gcttgatcgg cgactgccgg aggatctcga agtcgatgct
     1681 ctcctcgagg atcggcttga gcggattgta gccgagggga ttgagatcgt aaggcgaagc
     1741 cagccgggtc atgaggtcca tggcgacata gccgggcgaa tgatcgagcg accagcggcc
     1801 catgagccgg tcgagcggcg tgcgctggaa gggactgaag gccgcggcct cggacacgcg
     1861 ccgccagtag gcctcaaggg cctggcgcgc gccttccgcg ccgccctgca gccagcccga
     1921 ggcgagtacg gcagcattca tggcgcccgc ggaggtgccg gataccgcgt cgatcttcag
     1981 ccagctctcc tcgagcagcc gatcgagcac gccccaggtg aaggcaccat gcgagccgcc
     2041 gccctgcagc gccagatcga tgaggacgtc gggccggctc tgtccggaac gctttgcaga
     2101 tggacttcca ttcattaggt acaaggcctc ggatcatgca tcgccagcat ccttgttgca
     2161 ctgcagcatg aaacgcaagc ccaatctgcc ggacccgcga catgggccgg acgatctgtc
     2221 actccaagtt attgaatcgg ctggcgcacc cgacaggatt cgaacctgtg acctctgcct
     2281 tcggagggca gcactctatc cagctgagct acgggtgcct gagagccgga gggtctctat
     2341 agctcaaggc cgaggcgatc cgcaatggcg ggcggactgg ccggcgcggc gctggcggag
     2401 aagaagggct gcggcgcggc cggggtcacg gtcatcacag aactcgcccg ctgactggcc
     2461 cgaagcctgc acgcctctct cccaagccat gaaacctgtc ccgttccggg acggtaccgg
     2521 gagtccgcca tgtccgctgc cgccgcatca tcccgaatcg actgggtcga ctatgccaag
     2581 ggcctctgca tcattctcgt ggtcatgatg cattcgacac tgggcgtgga aaaggccact
     2641 ggccagctct cctggatgca cgaggtggtg gaatgggccc ggcccttccg catgcccgac
     2701 ttcttcctga tctccggcct gttcctggcc agccgcatcg accggccctg gcgcagctac
     2761 ctcgacagca aggtgcttca tttcgcctac ttctatgcct tgtgggtgac gatccagttc
     2821 gccgccaagg gctatggcat gactcaggag ctgggcgctg ccggcgcgct gcaggcctat
     2881 ctcatgagct tca
//
LOCUS       DBNGVR010000254         2494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3235748, whole genome shotgun sequence.
ACCESSION   DBNGVR010000254 DBNGVR010000000
VERSION     DBNGVR010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3235748"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="AC16A0_05440"
     CDS             complement(<1..628)
                     /locus_tag="AC16A0_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3104808.1"
                     /translation="MIVSHRHRFIFLKTRKTAGTSVELSLRPQCGPDDIVTPVTEDRA
                     APQCGGPRNYLHDRTGWSLADRIAWQLRGDLSLKSRPAIGFFGHVRGVDLRERLPARV
                     WRDYFKFTIERNPWDRQVSHYFWHMRRAGARKPSFGDYFRASAPLGNWEIYTERDALI
                     VDKVLRYETLQADLAATAAMLGLPDLQHVARAKTSHRPGTASYRDFYDD"
     gene            complement(625..2031)
                     /locus_tag="AC16A0_05445"
     CDS             complement(625..2031)
                     /locus_tag="AC16A0_05445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HIR3104809.1"
                     /translation="MKAAIINFTGGRENWGCQATSDEMLRFCRGIIARWPQSEIMLVP
                     CLPKHALDSFIERVHGKRIRRIFAAECPRPSDLRFLEGLVEQRFGAMIERVRSSDIVI
                     FQGEGTMGPKVHFRGLRFFALPFLAKHLWRKPVVSLNQSFYAARPSDLAVAANIYRHF
                     DIVALREGQSYALYRSLGIGEPLLCPDLAFRARPAIGARHDADAPYFCVTGSAAMDEY
                     DLGAYAVLIRDLAATTGLRPLLLYSKQDDSRLMAAMAEALPVGSYAAVNSKCMATYDG
                     ILPILRDARFVIGGRYHTAVTALAQATPVILLPANTHKNEGLATMLGIELPVHGLADR
                     RAILAEAAALMQPGNGRADAIRGALDTVAARQEAFAAYLRRFIAGGGREGGIDPLLTP
                     DLPAAKVAPQNAAIYAQVNAGRSHNLIGRLKGTQRQLGAWHLVRTQLKRKSLRKLKPY
                     VSMAVNRRIYDRGSETQP"
     gene            complement(2149..>2494)
                     /locus_tag="AC16A0_05450"
     CDS             complement(2149..>2494)
                     /locus_tag="AC16A0_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104810.1"
                     /translation="TYLEAAILEPQQGRISNCFVNNISRTGAKVSIRDEAVIPSVFWL
                     QISGDPDLRYCADIWRKDRNIGVDFAADRTLREIEDELARAKARMRLSDPERARMSHW
                     TAGHRPHHRSPD"
BASE COUNT          421 a          777 c          877 g          419 t
ORIGIN      
        1 cgtcgtcgta gaaatcccga tacgaggcag tgcccgggcg gtggctggtc ttggcccggg
       61 cgacgtgctg caggtccggc aggccgagca tggcggcagt cgcggcgagg tccgcctgaa
      121 gggtctcgta gcgcagcacc ttgtcgacga tcaaggcgtc gcgctccgta tagatctccc
      181 agttccctaa cggcgcgctg gcccggaaat agtcgccgaa actcggcttg cgcgccccgg
      241 cccggcgcat gtgccagaag tagtgcgaca cctgtcggtc ccacggattg cgctcgatgg
      301 tgaacttgaa atagtcccgc cacactctcg ccggcagtcg ctcgcgcagg tccacgcccc
      361 gcacatgccc gaagaagccg atggccggcc gcgacttgag gctgaggtct ccgcgcaatt
      421 gccaggcgat gcggtcggcc agcgaccagc cggtgcggtc atgcaggtag ttgcgcgggc
      481 cgccgcactg tggcgcggcg cgatcctcgg tcaccggcgt cacgatgtcg tcggggccgc
      541 actgcgggcg cagtgagagt tcaacgctgg taccggccgt cttgcgcgtc ttgaggaaga
      601 tgaagcggtg gcggtggctg acgatcacgg ctgcgtctcg gacccacgat cgtagatgcg
      661 gcggttgacg gccatcgaca catagggctt cagcttgcgc aggctcttgc gcttgagctg
      721 ggtgcggaca agatgccagg cgcccagttg ccgctgtgtg cccttcagac gcccgatcag
      781 gttatggctc cgccctgcat tcacctgcgc atagattgcg gcgttctgcg gcgccacttt
      841 ggcggcgggg agatcgggcg tgagcagcgg atcgattccg ccctcgcggc caccgcctgc
      901 gatgaagcgc cggagatatg cggcaaaggc ctcttgccgg gccgcaaccg tgtcgagggc
      961 cccgcggatt gcgtcggcgc ggccattgcc gggctgcatc agggccgctg cttccgccag
     1021 gatcgcgcgc cggtcggcaa ggccgtggac gggaagctcg atgcccagca tcgtggccag
     1081 cccctcgttc ttgtgggtgt tggccggcag caggatcacc ggcgtcgcct gggccagcgc
     1141 ggtgacggcg gtgtggtagc ggccgccgat cacgaagcgc gcatcgcgca ggatgggcag
     1201 gatcccgtca taggtcgcca tgcatttgct gttgacggcg gcgtagctgc ccaccggcag
     1261 ggcctcggcc atggcggcca tcaggcggga atcgtcctgc ttggagtaga gcagcagcgg
     1321 cctcagcccc gtcgttgcgg caaggtcgcg gatcagcact gcataggcgc cgagatcgta
     1381 ctcgtccatc gcggccgatc cggtcacgca gaagtagggc gcatcggcat cgtgccgcgc
     1441 gccgatcgct ggccgggcgc gaaaggcgag atccgggcac agcagcggct caccgatgcc
     1501 aagcgagcgg tacagcgcat aggactgccc ctcgcgcagc gccacgatgt cgaaatgccg
     1561 gtagatgttg gcggccacgg cgaggtcgga aggccttgcc gcatagaagg actggttcag
     1621 cgagacgacg ggcttgcgcc agaggtgctt ggccaggaag ggcagggcaa agaagcgcag
     1681 gccacggaaa tggaccttcg gacccatggt gccttcgccc tggaagatga cgatatcgct
     1741 cgaccgcaca cgctcgatca tcgcgccgaa gcgttgttcg accaggccct cgagaaagcg
     1801 cagatcggac ggccgcgggc attcggccgc gaagatgcgc cggatgcgct taccgtggac
     1861 gcgctcgatg aagctgtcca gtgcgtgttt cggcaggcag ggcacgagca tgatttcgga
     1921 ctgcggccag cgcgcgatga tcccgcggca gaaacgcagc atttcgtcgg aggtggcctg
     1981 gcagccccag ttctcccggc ctccggtaaa gttgatgatg gcggccttca tggggtgaac
     2041 ggagcgagga aacgggaatt gctgacgaag gctccttctc gccctggcaa aggtggaaag
     2101 ccttgacctc gatcaaatca gggcgtcccg cccggtcatg tcgcggggtc agtccggaga
     2161 ccggtgatgg ggtcggtgtc ccgcggtcca gtgtgacatg cgagcacgct ccggatccga
     2221 caggcgcatg cgggcctttg cccttgcgag ttcgtcttcg atctcgcgaa gcgtccgatc
     2281 ggcggcgaag tccacgccga tgttgcgatc cttgcgccag atatcggcac aatagcgaag
     2341 atcagggtcg ccgctgatct gcagccagaa gacggacggg atcaccgctt catcgcggat
     2401 gctgaccttg gcgccggtcc gcgagatatt gttgacgaag cagttcgaga tccggccctg
     2461 ctgcggctcg agaatcgccg cctcgagata ggtc
//
LOCUS       DBNGVR010000255         3826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3628109, whole genome shotgun sequence.
ACCESSION   DBNGVR010000255 DBNGVR010000000
VERSION     DBNGVR010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3628109"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="AC16A0_05455"
     CDS             complement(<1..677)
                     /locus_tag="AC16A0_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HIR3104811.1"
                     /translation="MTELTLAAEFPALDRSGWLKRVEAVLKGDSFESRLVNTTADGLR
                     LEVLYGQQHGPRAGRPFAQPWTISQRADHPDPHKANLQALDDLANGATGLTLVARESA
                     TARGFGLDMDALPRILHDIHLHAIALRLEGGGALAVAKLVESQPLDPERLDLCFGLKD
                     TQAAEALARRGFVGPFLNADGRHWHEAGATDAEELGAVLGEAVGYLRRLDSFNDAYLV
                     RAVSVTLA"
     gene            complement(755..1144)
                     /locus_tag="AC16A0_05460"
     CDS             complement(755..1144)
                     /locus_tag="AC16A0_05460"
                     /inference="COORDINATES: protein motif:HMM:NF022526.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HIR3104812.1"
                     /translation="MDELVTKISAATGLAPQKAEQALGIMLNLVKTQGNQNKVAELFA
                     KLPGADALVARHGGDGASSGGLLGMLAGGMMGGPLAAVTKLSAAGLSMDQIKQLGTIT
                     LDYAKAKAGDDLVKQVAGSIPGLSGYV"
     gene            1291..2703
                     /locus_tag="AC16A0_05465"
     CDS             1291..2703
                     /locus_tag="AC16A0_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR3104813.1"
                     /translation="MANRHGLVTGATGTGKTVTLQIMAEGFCRMGVPVFAADVKGDLS
                     GICMEGEPKDFLIERAKAIGFDDYGMEATPTIFWDLFGQQGHPIRTTVTEMGPLLLSR
                     FMGLSDAQEGALNIAFRIADKEGLALLDLKDLRALLKELGERGKEITTEYGNVNAQTI
                     GAIQRRLVVLEEQGGNSFFGEPALDITDLMRTTPNGRGYMNVLAADKLINSPQLYATF
                     LLWLMSELFERMPEVGDPEKPRLVFFFDEAHLLFAEAPAGLLQKIEQTVKLIRSKGVG
                     IYFVTQNPLDIPESVLAQMGNRIQHALRAYTPREQKAVQTAASTFRPNPAFKTVEVIT
                     QLGKGEALVSVLDLKGVPSMVERTMVRPPSSRMGPITPEERQKIVKSSPVYGQYEEAV
                     DRESAYEILQKAAAERAEAAQKAKEEIEALKAEARSSRGRDSATDRFVKNMAGGLGTT
                     LARSVGNALVRGILGGLTRK"
     gene            2814..3434
                     /gene="msrA"
                     /locus_tag="AC16A0_05470"
     CDS             2814..3434
                     /gene="msrA"
                     /locus_tag="AC16A0_05470"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3104814.1"
                     /translation="MRSVLIFLGVLLTVTPMAARAETAVLAGGCFWCVESDLDKLPGV
                     IRTTSGLAGGTSPSPTYKNPGDHIEAVKVEFDPAVISYDRLVHHFLRTIDVTDAGGQF
                     CDRGSTYVAALMPLDDKQGRIAQAALDEAARDLNREIATRIVASPNFHAVEDYHQDYY
                     LSSDRILTRFGLIRKSDAYRKYREACGRDQRVREVWGKAAYETLTQ"
     gene            complement(3449..>3826)
                     /locus_tag="AC16A0_05475"
     CDS             complement(3449..>3826)
                     /locus_tag="AC16A0_05475"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR3104815.1"
                     /translation="KGCLRVMAAIGMITDGPRRPAHAEPALSTSSSWVRAPESGILHT
                     NRRIGDRVGLKEVIGVVADPLGTQSAPIVSESDGIIIGRTNLPIVHRGDALFHIARSK
                     PAASKAKAKSDAGDPLYDEDEII"
BASE COUNT          694 a         1234 c         1215 g          683 t
ORIGIN      
        1 gcgagagtca cgctgacggc gcggacgaga taagcatcgt tgaagctgtc gagcctgcgc
       61 aggtagccca cggcctctcc aagcacggcg cccagctctt ccgcatcggt cgcaccggcc
      121 tcgtgccagt ggcgtccgtc ggcattgagg aacggaccga cgaagccgcg gcgggccagc
      181 gcctcggcag cctgcgtatc cttcaggcca aagcagagat ccagccgttc ggggtccagc
      241 ggttggcttt cgacaagctt ggccacggcc aaggcgccgc cgccctcgag ccgcagggcg
      301 atggcatgca ggtgaatgtc atgcaggatg cggggcaggg catccatgtc cagtccgaag
      361 ccccgtgccg tggcactctc ccgcgccacc agcgtcagtc cggttgcgcc attggcgagg
      421 tcatcgagcg cctggagatt ggccttgtgt gggtcgggat ggtcggcgcg ctggctgatg
      481 gtccagggct gcgcgaaagg ccgcccggcg cgcggcccgt gctgttgtcc gtacagcacc
      541 tccagccgga gtccatccgc cgtggtgttg accaggcgcg attcgaagct gtcccccttg
      601 agaaccgcct ccacacgctt caaccatccg gagcggtcaa gggccgggaa ttcggccgcc
      661 agggtcaatt cggtcatggg cctgtcatcc gccttgcgtt gcagcatgtg tagcgaagcc
      721 gcaggacccg tgcaaaccgc ccttggtcta acgctcagac atagccgctg agacccggga
      781 tcgatccggc gacctgcttc acaaggtcat cgcccgcctt ggccttggca tagtccagcg
      841 tgatggtccc cagctgcttg atctggtcca tggaaagacc tgctgcggag agcttggtca
      901 cggcggccag tgggccaccc atcatgccgc cggccagcat gcccaacagg ccgcctgaac
      961 tggcgccgtc gccgccatgc ctggccacca gcgcatccgc tccgggcagc ttggcaaaga
     1021 gctctgccac cttgttctga ttgccctggg tcttgaccag attgagcatg atgcccagcg
     1081 cctgttcggc cttctgcggc gccagccccg tcgctgctga aatcttcgtg accagttcgt
     1141 ccattgaaaa tcctgcctag catttgattg tcttggcaaa gtataccaga ttgatcaggg
     1201 tccaagccgc atattcgggg agaacgaaac gtgtccgaca acgcaatttt cctgggcaag
     1261 agcgagaagc cggaggaact cttcctgccc atggcgaacc gccacggcct ggtcaccggc
     1321 gccaccggca ccggcaagac ggtgaccctc cagatcatgg cggaaggctt ctgcaggatg
     1381 ggcgtccccg tcttcgcagc ggacgtgaag ggcgacctct ccggcatctg catggagggc
     1441 gagccaaagg atttcctcat cgagcgggcc aaggccatcg gcttcgacga ctatggcatg
     1501 gaagccacgc ccaccatctt ctgggacctg tttggccagc agggccaccc catccgcacc
     1561 accgtcaccg agatggggcc gctgctgctg tcgcgcttca tgggcctctc ggatgcgcag
     1621 gaaggcgcgc tcaacatagc cttccgcatt gccgacaagg aaggcctcgc ccttctcgat
     1681 ctgaaggacc tgcgggcact gctgaaggaa ctgggcgagc gcggcaagga gatcaccacg
     1741 gagtacggca acgtcaacgc acagaccatc ggcgccattc agcgccgcct ggtggtgctg
     1801 gaggagcagg gcggcaacag cttcttcggc gaacccgcgc tggacatcac cgacctgatg
     1861 cgcaccacgc cgaatggccg tggctacatg aatgtgctgg ccgccgacaa gctgatcaat
     1921 tcgccgcagc tctatgccac cttcctgctc tggctgatgt cggaactctt cgagcgcatg
     1981 ccggaagtgg gcgatcccga gaagccgcgc ctcgtcttct tcttcgacga agctcatctc
     2041 ctcttcgccg aggcgcctgc aggattgctg cagaagatcg agcagacggt gaagctgatc
     2101 cgttccaagg gtgtgggcat ctacttcgtc acccagaacc cgctcgacat tccggaaagc
     2161 gtgctggccc agatgggcaa ccgcatccag catgcgctgc gggcctatac gccgcgcgag
     2221 cagaaggccg tgcagacggc ggcttccacc ttccgtccca atccggcatt caagaccgtc
     2281 gaggtcatca cccagctggg caagggcgaa gcgcttgtct cggtgctcga tctcaagggc
     2341 gtaccgagca tggtggaacg gaccatggtc cggcctccgt cgagccgcat ggggccgatc
     2401 acccccgaag agcgccagaa gatcgtcaag tcgtcgccgg tctatggaca gtatgaggag
     2461 gccgtcgatc gcgagtcggc ctatgagatc ctgcagaagg ccgccgccga acgcgccgag
     2521 gcggctcaga aggccaagga agagatcgaa gccctgaagg ccgaggcccg atcctcgcgc
     2581 gggcgggatt ccgcgaccga ccgtttcgtc aagaacatgg cgggagggct tggcaccacc
     2641 cttgcgcgct cggtgggcaa tgcactggtg cgcggcatcc tcggcggact gacgcggaag
     2701 tagcatctgt tcaaggcgcg tccttgtctt cacctgatcg tgcgcaaggg tgatcctgcc
     2761 gctgatccat tgctgctatt gacgagtatc agtcggattc aaaggagact gcgatgcgat
     2821 ctgtcctgat ctttctgggc gttcttctga cggtcacgcc gatggcggca cgcgccgaaa
     2881 cggctgtgct ggccggcgga tgcttctggt gcgtggagag cgatctcgac aagctgcctg
     2941 gcgtgatccg gacgacctcg ggtctggcag gtggcacgtc gccatcgccg acatacaaga
     3001 acccgggcga tcacatcgaa gcggtgaagg tcgagttcga ccccgccgtg atctcgtacg
     3061 acagattggt gcatcacttc ctgcggacga tcgatgttac agacgcgggc gggcagttct
     3121 gcgatcgcgg cagcacctat gtcgcggcgc tcatgccgct cgacgacaag cagggccgca
     3181 ttgcgcaagc cgccctggac gaagccgccc gtgatctcaa ccgcgagatc gcgacgcgca
     3241 tcgtcgcatc tccgaacttc catgccgtcg aggactatca ccaggactac tatctgagtt
     3301 cggatcggat cctcacgcgc ttcggcctca tcaggaagtc cgatgcctac aggaagtacc
     3361 gcgaggcctg tggccgcgac cagcgtgtgc gggaagtctg ggggaaggct gcctacgaga
     3421 cgctgacgca gtagtttcgg gtcatctgtc agatgatctc gtcttcgtcg taaagaggat
     3481 cgcctgcgtc ggatttcgcc tttgccttgc tggcggcggg cttggacctg gcgatgtgaa
     3541 acagcgcatc gccgcgatgc acgatgggca gattggtgcg cccgatgatg ataccgtcgc
     3601 tctccgagac gatgggagca gactgcgtgc ccagcggatc ggcgaccacg ccgatcacct
     3661 ccttcagccc cacccggtcg ccgattcggc ggttggtgtg caggattccg ctctccggcg
     3721 cccgcaccca ggaactcgac gtcgacagcg cgggctcggc atgcgccggg cggcgcggcc
     3781 catcggtgat catgccgatg gcggccatca cgcggaggca cccctt
//
LOCUS       DBNGVR010000256         7154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4022084, whole genome shotgun sequence.
ACCESSION   DBNGVR010000256 DBNGVR010000000
VERSION     DBNGVR010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4022084"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="AC16A0_05480"
     CDS             complement(<1..1158)
                     /locus_tag="AC16A0_05480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3104816.1"
                     /translation="MADMTGEDALMKSQESPAQGISTGSESTPAPSSGWQRELLVHFL
                     RNQIRVTPAMPVLALLLGITAMQWASPSRVIVWMIGAFACQAIQTYLCQLYFKRERSY
                     DEQRDWIGIMSAAELLTGICWFLPLFLFWTEANHSQQIYLIASVMVVIAVRLLIVNSF
                     MPVLIAGTGVLTIGVAMRCVWEAEPLYIALGATIVALEAFLLMVARNLQETARDMLIF
                     KAQKDTLIADLQRAKAKAEEQQQRAEEASRAKSEFLATMSHELRTPLNAIMGFSEILK
                     HEMFGPLAVKAYKDYADDIHHSGNYLLALINDILDLSRIEAGRRDVQDEPVSVLIAVE
                     EVNHLLLMKAADKKIEVFVEVSDTLPRLMADRRAISQILINLMSNAIKFTPA"
     gene            1319..1909
                     /locus_tag="AC16A0_05485"
     CDS             1319..1909
                     /locus_tag="AC16A0_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR3104817.1"
                     /translation="MGRALPHEPRPVLQPSATARLLIAGQAPGTRVHQSGRPFTDPSG
                     DRLRQWLGVSMEDFYDPARTAILPMGFCFPGLSAKGADLPPRRECALAWRGACMAAMP
                     QVELVICLGAHAMRWHMGNAFPGSMDAAVRDWRSGLALSPQVIALPHPSWRNNAWLKR
                     NAWFEADLLPELRAAVSRVLPAAPVTAMRAAAQLDE"
     gene            1911..3020
                     /locus_tag="AC16A0_05490"
     CDS             1911..3020
                     /locus_tag="AC16A0_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006137959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR3104818.1"
                     /translation="MPQVTRDDIVSVLKRIAAPDGRGNLVSAGLVSEISIQDGTVMFA
                     ISTDPQHVKTMELLRAAVEKAVAAIPGVSKAIVALTAERAPQREPGPRAQAAQRGQAV
                     PGIRHIVAVASGKGGVGKSTTAINIALGLKALGLRVAVLDADVYGPSMPKLFGLSGKP
                     QAADSEGKKMKPMQRFGVELMSIGFLVAEDTPMIWRGPMVMSALTQLLRETAWSETDV
                     MIVDMPPGTGDAQLTLAQQTPLSGAVIVSTPQDLALIDARKGLNMFRKVNVPVLGIVE
                     NMSYFVCDGCGKRHEIFGHGGAREEAEKLGLTFLGEVPLDRDVRLRSDRGEPVTATVP
                     DGQHAAIYRDIARKVWEAVEQGGLARPAPRIVMDS"
     gene            3117..3782
                     /locus_tag="AC16A0_05495"
     CDS             3117..3782
                     /locus_tag="AC16A0_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104819.1"
                     /translation="MAKLMWIAGILLAAVGIALGLMVFFSTAQMVAWGVTLDTASVLL
                     VGGLLALGLGGVINAVDVLGERYAAVDIVAAPAKAAATPIPEYGRRAAEAAGAAAAIT
                     EVSPEVKDTIDKIERAKQDLSKAFGDKTAVTGVSVTETVVASAPSEAAAEAAIEEIEE
                     AIDEAQGGEDQLYVVEERTIRNRPARILSDGTVEAETDEGWMRFENLEHLDEYLEAMS
                     PST"
     gene            complement(3787..4413)
                     /locus_tag="AC16A0_05500"
     CDS             complement(3787..4413)
                     /locus_tag="AC16A0_05500"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3104820.1"
                     /translation="MSEFKLHCFGESGNSYKPALMLEICRLDWQPVWIDFFNGATRDP
                     AFRHDVNEMGEAPVLEHGTLRLTQSGVILDYLAEQTGRYGAENATERREIWRWILFDN
                     HKFTASLATLRFLVNFAKTGETPVTEFLRARAITAIKIVDGHLADNSFIIGKRLTIAD
                     VSLCGYVFYGDELTIPLASYSNLQKWMDRIRALPGWKHPYDLMPRAPA"
     gene            complement(4499..5509)
                     /locus_tag="AC16A0_05505"
     CDS             complement(4499..5509)
                     /locus_tag="AC16A0_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3104821.1"
                     /translation="MAFFSRTTLSDRQEAAVNKTLQNLRTQRRNRFRVLACIDGTDES
                     FVTVRFGARYCVQSNCDLIILFVRPIDQGLHSGGLQVRLARQNMLDAGFELPGVRHLR
                     RAIEILKEEGIDVSAWPQEAEHQDAWGDAAGDNKIEYLSPEGRSVVVKLKTAPDVANG
                     ILDQYELGPYNLIILGEPSRWRGEVAAFFDSGIVQTVTTMAPCSVLVARQSFAKSGYF
                     ICTDGSSRSMQAVRRSAVLATVTGEPITLFSVATTESGRSAAEEAVANAKALLRAMKI
                     NVAETRVAVGTPAEEIIESGSLYKVIVVTDEGRSRFQRLFRGSIATDVVRGARTSVLD
                     VR"
     gene            complement(5519..7075)
                     /locus_tag="AC16A0_05510"
     CDS             complement(5519..7075)
                     /locus_tag="AC16A0_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3104822.1"
                     /translation="MSTAAPQFQLARDSLQAREPSAGIGRIRRRGYQRFLHLMSFVDL
                     AFVWRRRWFFITMILGFALMSAPTPAGLTHEGQIVLVMSLMATMLFITEAIPLPTVPL
                     LIIVGEVVLLGVDSTEVARSLMTDSVLFIMGSLMLAVAIVKQRLDKRIAWWIVRITGT
                     NVYWISFGIVTVCGVLAAFIGEHTVAAMMLPVGVTLISLTSNDPKKVHNLAAVILFSI
                     SYGCSIAGVATPSGGARNAIIISYWKDFFYNPLDPETRRFLMDYVTWTIYAFPMFLLR
                     LPIVAALLIYTFKPEVTDMSRAISRLRAQVSMQGPMRGNEWMTILIFAITILGWVTMS
                     NTLGLGIIAVAGAAAFLVFGLVRWEDINSGVNWGVVLLYAAAISLGVEMKATGAAEWV
                     AASVFDVMKNLSIGSGIGFNAAISLLTIGVSNTMTAGAAVAVLAPVVLKTAVAAGEDP
                     ISAGFVTAISSAFGYLTPAAQPAFTIIFASGYLKGPDFFKIGIRMMLISFAVLLLLAT
                     VYWPFLQHMS"
BASE COUNT         1357 a         2163 c         2379 g         1255 t
ORIGIN      
        1 agcgggggtg aacttgatcg cgttcgacat gagattgatc aggatctggc tgatggcacg
       61 gcggtcggcc atgagccgcg gcagcgtatc ggagacttcg acgaagacct cgatcttctt
      121 gtcggcagct ttcatcagga gcaggtggtt gacctcctcg acagcgatca ggacgctcac
      181 gggttcatcc tgcacgtcgc ggcggccagc ctcgatgcgg gacagatcga gaatgtcatt
      241 gatcagcgcc aacagatagt tgccggagtg atggatatcg tcggcatagt ccttgtaggc
      301 cttgaccgcg agagggccga acatctcgtg cttgaggatt tcggagaatc ccatgatggc
      361 gttcagcggc gtgcgcagct cgtggctcat ggtcgccagg aactccgact ttgcccggct
      421 cgcctcttcg gcgcgttgct gctgctcctc ggcctttgct ttggcgcgct gcaggtcggc
      481 gatcaacgta tccttctggg ctttgaagat cagcatgtcg cgcgcagttt cctgaagatt
      541 gcgcgcgacc atcagcagga aggcttcaag cgcgacaatg gtggcgccga gcgcaatgta
      601 gagaggttcc gcttcccaga cgcagcgcat cgccacgccg atggtcaaaa cgccggtgcc
      661 cgcgatcagc accggcatga agctgttcac gatgaggagg cggacggcga tcaccaccat
      721 cacggaggcg atgaggtaga tctgctggct gtgattggct tccgtccaga acaggaagag
      781 cggcaggaac cagcagatgc cggtcagcag ctctgcggcg gacatgatgc cgatccagtc
      841 gcgctgctcg tcgtagctgc gctcgcgctt gaagtagagt tggcaaaggt aggtctgaat
      901 ggcctggcat gcgaaggcgc cgatcatcca gacgatcacc cgggacgggc ttgcccactg
      961 catcgcggtg atccccagca gcagggcaag caccggcatt gccggggtga cacgaatctg
     1021 attgcgaagg aagtggacaa gcagttccct ttgccagccg ctgcttgggg caggcgtcga
     1081 ctcgctgccg gtcgaaatgc cctgtgcggg cgactcctgg ctcttcatga gagcgtcctc
     1141 acctgtcatg tccgccatga cccgtccctt ggcatcgcga tacagtccaa attcaccgct
     1201 acaccatagg tcactttcgc taatgtcggc tttggaacac cggtaaactt tgaagcaaac
     1261 tcgcctcacg gacctgacgg atcaaatcct tgcctgccgc atctgccgcg ataggccgat
     1321 gggccgtgcg ctgccgcacg agccgcgtcc ggtcctccag ccaagcgcaa cggcgcggct
     1381 gctgattgcc gggcaggcgc cggggacgcg cgttcaccag tctggccggc cctttaccga
     1441 tccctcgggc gaccggctgc ggcagtggtt gggcgtctcc atggaggact tctatgatcc
     1501 ggcgcggacc gccatcctgc ccatggggtt ttgctttccg gggctcagcg cgaagggggc
     1561 agacctgccg ccgcgccgcg aatgcgcact ggcctggcgt ggcgcctgca tggcggccat
     1621 gccccaggtc gaactggtga tctgcctggg tgcccatgcc atgcgctggc acatgggtaa
     1681 cgcctttccc ggcagcatgg atgccgcggt gcgggactgg cgcagcggac tggcgttgtc
     1741 gccgcaggtc attgccctgc cgcatccgtc atggcgaaac aacgcatggc tcaagcgcaa
     1801 cgcctggttc gaagccgatc tcctgccaga gcttcgagcc gctgtaagcc gtgtcttgcc
     1861 cgcggccccg gtcacggcta tgcgggccgc agcacagctg gatgaatgac atgccgcagg
     1921 ttacaaggga cgacatcgta tcggtgctca agcgcatcgc cgcaccggac gggcgcggca
     1981 atcttgtgtc ggccggactg gtatcggaga tctccataca ggacggcacg gtgatgtttg
     2041 ccatcagcac cgatccgcag cacgtcaaga cgatggagct cctgcgggcc gcggtcgaga
     2101 aggccgtggc ggcgataccc ggcgtttcca aggcaattgt cgccttgacc gcagaacgcg
     2161 ccccgcaacg cgagcctggc cccagggccc aggcggcaca gcgcggccag gccgtgcccg
     2221 gcatccggca tattgttgcg gtcgcctcgg gcaagggcgg cgtgggcaag tcgacaacgg
     2281 ccatcaacat tgcgctcggg ctcaaggcgc tgggcctgcg agtggccgta ctcgatgccg
     2341 atgtctatgg cccgtcgatg ccaaagctgt tcgggttgtc gggcaagccg caggccgccg
     2401 acagcgaagg caagaagatg aaaccgatgc agcgtttcgg ggtggagttg atgtccatcg
     2461 gctttctcgt ggccgaggac acgccaatga tctggcgggg ccccatggtg atgtcggcgc
     2521 tcacgcaatt gcttcgcgag acggcctgga gcgaaaccga cgtgatgatc gtcgacatgc
     2581 cgcccggcac cggcgatgcc cagctgaccc tggcgcagca gacgccgctg tcaggcgcgg
     2641 tgatcgtgtc cacgccgcag gatcttgcat tgatcgacgc ccgcaagggc ctcaacatgt
     2701 tccgcaaggt caacgtcccg gtcctgggca tcgtggagaa catgagctac tttgtctgcg
     2761 acggttgcgg aaagcgtcat gagatcttcg gtcatggcgg tgcgcgcgaa gaggcggaga
     2821 agctgggctt gaccttcctc ggtgaagtgc cgctggaccg ggacgtgaga ctgcggtccg
     2881 atcgcggcga gccggtgacc gccacggttc ctgatggtca gcacgcggcc atctatcggg
     2941 atattgcacg aaaggtctgg gaggcggtcg aacagggggg gcttgcccga ccggcgccac
     3001 gaatcgtcat ggacagctaa gcgcttccaa tccttggaaa ttgatgaaat attaaccacg
     3061 gcgacggttc atgatagctt gggaccgctg ccgaaaatcg agttgagggg cttgccatgg
     3121 caaaactgat gtggatcgcc ggaattctgc tggctgcggt aggaattgcg cttggcctga
     3181 tggtcttctt ctcgacggcg cagatggtcg cctggggcgt aacgcttgat actgcgtccg
     3241 tgcttctggt gggaggactg ctcgcgcttg gccttggcgg tgtcatcaat gcggtggacg
     3301 tgctgggcga gcgatacgcg gccgtcgaca tcgtggcagc accggccaag gcggcggcca
     3361 ctcccatccc ggagtatggc cgccgggctg ccgaggccgc gggagctgcc gcagccatca
     3421 cggaggtgtc gcccgaagtg aaggacacca tcgacaagat cgaacgggcc aagcaggacc
     3481 tgagcaaggc cttcggcgac aagaccgcgg tgaccggcgt gagcgttacc gagaccgtcg
     3541 tggccagcgc gccgtcggag gccgcggccg aagcggcgat cgaggaaatc gaggaagcga
     3601 tcgacgaagc gcagggcggc gaagatcaac tctatgtcgt ggaagagcgg acgatccgca
     3661 accggcccgc ccgcattctg tcggacggca ctgtcgaagc tgaaacggat gaaggctgga
     3721 tgcggttcga gaacctcgag cacctggacg agtatctcga ggccatgtcg cccagcacct
     3781 gacccgtcac gccggcgcgc gcggcatcag atcataggga tgcttccagc cgggaagtgc
     3841 ccggatgcga tccatccact tctgcagatt ggaatagctg gcgagcggga tggtcagctc
     3901 gtcaccatag aacacatatc cgcacagcga gacatcggcg atcgtcagcc gcttgccgat
     3961 gatgaaggaa ttgtcggcca gatgcccgtc gacgatcttg atggcggtga tggcgcgggc
     4021 ccgcaggaat tccgttaccg gagtctcgcc cgtcttggcg aagttgacga ggaagcgcag
     4081 ggtcgcgaga ctcgccgtga acttgtgatt gtcgaagagg atccagcgcc agatttcgcg
     4141 tcgctcggtg gcattctccg cgccgtaccg gccggtctgt tcggccagat agtccaggat
     4201 cacgccggac tgtgtgaggc gcaatgtacc atgttccagg accggggcct cccccatttc
     4261 attgacatcg tgccgaaagg ccgggtcccg cgtggcgccg ttgaaaaaat caatccagac
     4321 cggctgccag tccagcctgc agatctcgag catgagggcc ggcttgtagg agttccccga
     4381 ttccccgaag caatggagct tgaactcgga cattcggcat tcccttccgg ctggcgacag
     4441 atcgggcgac agcctgcaca agatcgggac ggttgggaag gccccggcgc gggcgaacct
     4501 aacggacatc caggacggag gtgcgcgcgc cgcggacgac atcggttgcg atcgacccgc
     4561 ggaacaggcg ctggaatctg ctgcggcctt cgtcggtcac gacgatgacc ttgtagagcg
     4621 atcccgactc gatgatctct tccgctggcg tgcccacggc cacgcgcgtc tcggccacgt
     4681 tgatcttcat ggccctgaga agagcttttg cattggccac ggcttcctcg gcggcgctcc
     4741 ggcccgactc ggtcgtcgcc accgaaaaca gggtgatcgg ctcacccgtc acagtggcga
     4801 gcacggcgga ccggcgcacc gcctgcatcg agcgcgagga gccatcggta cagatgaagt
     4861 agcccgactt cgcgaagctt tggcgggcca cgaggacgga gcagggcgcc atggtggtga
     4921 cggtctgcac aatgccggag tcgaagaagg ccgccacctc gccgcgccag cgcgacggtt
     4981 cgcccaggat gatgaggttg tagggtccga gctcgtactg atcgagaatc ccattggcca
     5041 cgtccggcgc cgtcttcagc ttgaccacaa ccgaacggcc ttcggggcta aggtactcga
     5101 tcttgttgtc gccggcggcg tccccccagg catcctggtg ttcggcctcc tgcggccagg
     5161 cggagacgtc aattccctct tccttgagta tctcgatcgc gcggcggagg tggcggactc
     5221 cgggcagttc gaagcccgca tccagcatgt tctggcgggc gagccgcacc tgcaggccgc
     5281 cggaatgaag cccctggtcg atgggtctga caaagaggat gatcaggtcg cagttgctct
     5341 gtacgcagta tcgcgcaccg aagcgcaccg tcacgaagga ttcgtcggtg ccatcgatgc
     5401 aggccagcac ccggaaccgg ttgcggcgct gggtccgcag gttctgcagg gtcttgttta
     5461 ccgctgcttc ctggcggtcc gagagggttg tgcggctgaa gaacgccatg cgcccagcct
     5521 atgacatgtg ctgcaggaat ggccagtaca ccgtggccag gagcaggagc accgcaaagg
     5581 agatcagcat catgcggatg ccgatcttga agaagtcggg acccttcaga taacccgagg
     5641 cgaagatgat ggtgaaggcc ggctgcgccg ccggcgtgag gtagccgaag gcagatgaga
     5701 tggcggtcac gaagccggcg ctgatcggat cctcgccggc agccacggcg gtcttcagca
     5761 ccaccggagc aagcacggcc acggcggcgc cggcggtcat ggtgttcgag acgccgatgg
     5821 tcagcagcga aatggctgcg ttgaagccga tgcccgatcc gatcgacagg ttcttcatca
     5881 cgtcgaagac gctggccgcc acccattcgg cagcacccgt cgccttcatc tcgacaccga
     5941 gcgagatcgc cgcggcatag agcaggacca ccccccagtt gacgccggag ttgatgtctt
     6001 cccagcgcac caggccgaag accaggaagg cagctgcgcc ggcgacggcg atgatgccca
     6061 acccgagcgt attcgacatc gtgacccagc ccaggatggt gatggcgaag atcaggatgg
     6121 tcatccattc attgccgcgc atgggaccct gcatcgagac ctgcgcccgc aaccggctga
     6181 tggcccggga catgtccgtg acttccggct tgaacgtgta gatcagcagt gccgccacga
     6241 tgggcaggcg gagcaggaac atcgggaagg cgtagatggt ccaggtgacg tagtccatca
     6301 ggaagcggcg cgtttccgga tccaacggat tgtagaagaa gtccttccag taacttatga
     6361 tgatggcgtt ccgggcgccg ccggacggcg tcgccacacc cgcgatggag cagccatagg
     6421 aaatcgagaa caggatgaca gccgccagat tgtggacctt cttcgggtcg ttcgacgtga
     6481 gcgagatcag cgtgacgccc accggcagca tcatggcggc aaccgtgtgc tcgccgataa
     6541 aggcggcgag aacgccgcag accgtgacga tgccgaagct gatccagtag acgttcgttc
     6601 cggtgatccg cacgatccac caggcaatgc gcttgtcgag ccgctgcttg acgatggcca
     6661 cggcaagcat cagcgagccc atgatgaaga gcacggaatc ggtcatcagg ctgcgggcga
     6721 cttccgtgga gtctacgccg agcaggacca cctcgcccac gatgatgagc aatggtaccg
     6781 tgggcagcgg aatggcctcg gtgatgaaca gcatggtcgc catgagcgac atgaccagaa
     6841 cgatctggcc ttcgtgggtc aggcccgcgg gggttggcgc cgacatcagc gcgaaaccca
     6901 ggatcatggt gatgaagaac cagcgccggc gccagacaaa ggcgaggtcc acgaaggaca
     6961 tgaggtgcag gaagcgctgg tagccgcggc gcctgatccg cccgatgccg gccgaaggct
     7021 cgcgcgcctg caggctgtcg cgggccagct gaaactgcgg ggcggccgtg ctcatggcgt
     7081 aacccttgct ccatgggcat gcgccgtgag acagtgccga tgattccggt tttccggccc
     7141 catggtgttg tctt
//
LOCUS       DBNGVR010000257         3382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_485938, whole genome shotgun sequence.
ACCESSION   DBNGVR010000257 DBNGVR010000000
VERSION     DBNGVR010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_485938"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1277
                     /locus_tag="AC16A0_05515"
     CDS             456..1277
                     /locus_tag="AC16A0_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HIR3104823.1"
                     /translation="MRTGHNKQRNRNRHRSSGGGGGGGGGGGGGNPLSRVYESNGPDV
                     KVRGTAQTIADKYLQLGRDAQVSGDNVTAESYYQFAEHYLRIVAAAQAYNQQMQQQFR
                     RPDDDQDEEGDEDGAEIQAQPRAADQGQGEQPRESDGERQQQPQQNFRQNRDQRDDRG
                     NRDNRDRNRPRFQDRRDGQPAGNAEARGNVQPREAAPPARAESTEDQSQWEAPSFLRR
                     PAPLPATETAGDAQDEAAAAPAAERKPRRPYTRREKPVDAEEGRAVEATPEPAAD"
     gene            complement(1283..2080)
                     /locus_tag="AC16A0_05520"
     CDS             complement(1283..2080)
                     /locus_tag="AC16A0_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR3104824.1"
                     /translation="MGNEAPQINPTGLGAYSPSEVARRIEEAGIAKSRLPAVPLMTLA
                     VLAGSFIALGAAAFTAVMVGTDLSYGPARLLGGLAFSLGLVLVVVAGAELFTGNTLIV
                     MAWVDRKIRLRDLLRNWLFSFSGNAAGALAIVALMALAGLLKGQIGELAIRIAEAKAS
                     LGWTEALARGILCNMLVCLAVWLTFAARDVAGKILAILLPISAFVLLGFEHSIANLYL
                     IPAGWMAGASVGWGGFLGNLVPVTIGNIIGGAGGVALTYRLAYRPGG"
     gene            2333..>3382
                     /locus_tag="AC16A0_05525"
     CDS             2333..>3382
                     /locus_tag="AC16A0_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR3104825.1"
                     /translation="MDFDKYTERSRGFIQSAQSLALREGHQRFTPEHLLKVLLDDEEG
                     LAAGLIKAAGGDSRIALRDVEAALKKMPKVSGGGAGQVYLSPELARVFDAAQKIADKA
                     GDSYVTAERLLQALAVEPSEAAKALKAAGATAQGLNEAINAIRKGRTADTASAEQGYD
                     ALKKYARDLTEAAREGKRDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEAGVGKTAIAE
                     GLALRIIKGDVPESLKDKKLLALDMGSLIAGAKYRGEFEERLKAVLSEVTAAAGGIIL
                     FIDEMHTLVGAGKADGAMDASNLLKPALARGELHCIGATTLDEYRKHVEKDPALERRF
                     QPVFVGEPTVENTIAI"
BASE COUNT          676 a         1097 c         1142 g          467 t
ORIGIN      
        1 ggtcgagatc ggcgccggcc aggcggacga tgtgtcagcc ctcttcgctg cggccgggtt
       61 tatcaatgcc gaacgatttc aagatcttgg gggccatgac cggtgcctgt cgttcaggct
      121 tggttaacgc tgggtcctgt ggacatggcc aaacaaaagc attggaaatc agccgctact
      181 ccgctagata cggtgcgtag agtttttctg ccctttgtga tggcgccggg gcgaagcgtg
      241 gatgaccggc caggaactgg cggtccgaca tactaaatcc ggagcaaatc gggggattgg
      301 catggcctgc ggcaggcccg gccagcggca gcgactcaca gcaaccaacg ggttcgtatt
      361 tggaaaagct caagtcttga gcatcgatcc gacaggttcc gtcatgcatg tgcgggagcc
      421 caagggcgaa cagaacccag cgaccaggac acgatatgag aaccgggcac aacaagcagc
      481 gtaaccgtaa ccgccatcgc agcagcggcg ggggtggagg cggaggcggc ggcggaggcg
      541 gcggtaatcc gctgagccgg gtctatgaga gcaacgggcc ggatgtgaag gtccggggca
      601 cggctcagac catcgccgac aaatatctcc agctgggccg cgatgcccag gtctcgggcg
      661 acaatgtcac ggccgagagc tactaccagt tcgccgagca ctatctccgc atcgtggccg
      721 cggcccaggc ctataaccag cagatgcagc agcagttccg ccgccctgac gacgatcagg
      781 acgaagaggg cgacgaggat ggtgccgaga ttcaggccca accgcgcgct gccgatcagg
      841 gccagggcga acaaccgcgg gagagcgacg gcgagcgcca gcagcagccg cagcagaatt
      901 tccgccagaa ccgcgatcag cgcgacgacc gcggcaaccg cgacaaccgt gaccgcaacc
      961 ggccccgctt ccaggaccgt cgtgatggcc agccggccgg caacgccgaa gcccgtggca
     1021 atgtgcagcc ccgtgaagca gcgccgcctg cgcgtgccga atccactgag gatcagagcc
     1081 aatgggaagc gccgagcttt ctgcggcggc cggcgccatt gccggccacg gagacagcgg
     1141 gcgatgcgca agacgaggct gcagcggcac cggccgccga acgcaagccg cgccggccct
     1201 atacccgccg cgagaagccg gtcgacgccg aagagggccg cgccgtcgaa gcgacgccgg
     1261 aacccgccgc cgactgacgc cgtcagccac ccggccggta ggccaggcga taggtgaggg
     1321 ccaccccgcc cgcaccgccg atgatgttgc cgatggtgac cggaacgagg ttgcccagga
     1381 agccacccca tccgaccgac gccccggcca tccagccggc ggggatcagg tagagatttg
     1441 caatcgagtg ctcgaatccg agcaggacga aggccgagat cggcagcagg atggccagga
     1501 tctttccggc cacgtcccgg gcggcaaagg tgagccagac ggcgaggcag accagcatgt
     1561 tgcagagaat cccccgggcc agcgcctcgg tccagccgag gctggccttg gcttcggcga
     1621 tgcggatcgc cagctcgccg atctgaccct tgagaagccc cgccagtgcc atcagggcga
     1681 cgatcgccag ggcgccggcg gcatttcccg aaaaggaaaa gagccagttc cggaggagat
     1741 cgcgcaagcg gatcttgcgg tcgacccagg ccatgacgat cagcgtgttg ccggtgaaga
     1801 gttctgcacc ggccacgacc accagcacca gtcccagcga gaaggcaaga ccgcccagca
     1861 gccgggccgg gccatagctc agatccgtgc caaccatgac ggccgtgaag gccgccgcgc
     1921 ccagggcaat gaagctgccc gcaagaaccg caagcgtcat gagaggcacg gccggaagcc
     1981 gcgacttggc gatgcccgcc tcctcgatgc gacgggccac ttcggacggg ctgtaggcgc
     2041 ccagtccggt cgggttgatc tgcggagctt cgtttcccat gtgatccttc tgcgcgttgg
     2101 cgagagatca tgtccttgat ccaccgcaag agtttcgacc gggcctgggg ccgtccgcgg
     2161 ccgatcttgt cgacgcaagg cagacttacc atattagttc ccgagcaagg ggttctgcct
     2221 caaggatgcc gtcgaggatc cgggagcgcg gttgccccgc caaccgttga cggcgtttgc
     2281 cgggtgcccg cacggggcct tgcaggcgtg ccgaggagag gaaagacaca tcatggattt
     2341 cgataaatac accgaacggt cccgcggttt catccagtcg gcccagtcgc tggccctgcg
     2401 ggaaggacac cagcgcttca ctcccgaaca tcttctgaag gtcctgctcg atgacgagga
     2461 aggacttgcc gccggcctga tcaaggccgc gggcggcgac tcgcgcatcg cgcttcgcga
     2521 cgtcgaggcc gcattgaaga agatgccgaa ggtgtccggt ggcggcgccg gccaggtcta
     2581 cctgtcgccc gagctggcgc gggtcttcga cgcggcccag aagatcgccg acaaggccgg
     2641 ggacagctat gtcactgccg agcgtctgct gcaggcgctg gccgtggaac cgagcgaggc
     2701 cgccaaggcg ctcaaggccg cgggcgccac tgcccagggt ctgaacgagg ccatcaatgc
     2761 aatccgcaag ggccggaccg ccgacacggc ctcagccgaa cagggctatg atgcgctgaa
     2821 gaaatatgcc cgcgacctca cagaggccgc gcgtgaaggc aaacgcgatc cggtcatcgg
     2881 ccgcgatgag gagatccgcc gcaccatcca ggtgctgtcg cggcgcacca agaacaatcc
     2941 ggtgctgatc ggcgaagccg gcgtcggcaa gacggcgatc gccgaaggcc tggccttgcg
     3001 catcatcaag ggcgacgtgc ccgagagcct gaaggacaag aagctcctgg cgctcgacat
     3061 gggctccctc attgccggcg ccaagtaccg tggcgagttc gaggagcggc tgaaggccgt
     3121 gctctcggaa gtgacggcgg ctgcgggcgg catcatcctg ttcatcgacg agatgcacac
     3181 gctggtcggc gccggcaagg cggacggggc gatggacgcc tccaacctgc tgaagcccgc
     3241 gctcgcccgc ggcgagctgc actgcattgg cgccaccacg ctcgacgagt accgcaagca
     3301 tgtcgagaag gatccggcgc tcgagcggcg cttccagccc gtcttcgtcg gcgagcccac
     3361 ggtggagaac accatcgcca tc
//
LOCUS       DBNGVR010000258         3680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1862227, whole genome shotgun sequence.
ACCESSION   DBNGVR010000258 DBNGVR010000000
VERSION     DBNGVR010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1862227"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="AC16A0_05530"
     CDS             <1..949
                     /locus_tag="AC16A0_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3104826.1"
                     /translation="GQEARTGRSSSWPRLARALNEPASVRPYAKPRPRPPLAARPRQL
                     SVTRIETLIRDPYAIYARHVLKLQPVEPIVARADPARRGMVIHEAISDFLQAYPDALP
                     PDALAELLRLGHAHFAAVLGDPAVFSFWWPRFERIADWLVEQERQQRPGIAHIHAEIE
                     GRIALSIADAAFHLTCRADRVDVLKDGTARIIDYKTGAVPSAREVRAGLAPQLTLQAA
                     ILARGGFTAVAPMEVSAVSYVKLSGGDPAGEDKGPDLGADLPQVVAQHVAGLVKLIAA
                     YRNADQPYLPRAMMKHEDDASDYDHLSRYREWALAGEAR"
     gene            946..>3680
                     /gene="addA"
                     /locus_tag="AC16A0_05535"
     CDS             946..>3680
                     /gene="addA"
                     /locus_tag="AC16A0_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HIR3104827.1"
                     /translation="MTAQPTASDRQREASDPRSSAWVNANAGSGKTRVLVDRVIRLLL
                     DGAEPSKIMCLTFTKAAAAEMSNRLFERLSAWVVLPEPELRAQLAELGVDDGDPATLR
                     RARRLFTRALETPGGLKIQTIHAFCERVLQAFPVEAGVVPRFAVLDERGQRDLLLAAR
                     GEVLDEALKQPAGDLGLAVADVVRRVQPDGFDALMQHLLSRRAELFGLLATADGVDAV
                     MTVLGAHLGIDPAETAQQVKAGLALDLPRYRDIADALATGSTADRKSLDQLAPVLLRP
                     TPNLLDLQTFYLTQKGEARSRMATNAVLARLPWLAGFAAGEQQRLLAALGRLADLERL
                     AATRALLILAGAVMRRFEAAKRRQGAYDFDDLIIRTAELLATRSDAQWVLFKLDGGIE
                     HLLVDEAQDTSPAQWRIVTALTQEFFAGAGRHGEKRRTIFAVGDRKQSIYSFQGADPD
                     IFAEVQMAFAQRLGEAGQRLRDVDFTVSFRSAPEILQAVDKVFAPDAVARRGLDGAAT
                     RDWVHESNRRAIRGMVEIWPLVAPLDKEDGDVWTAPVDREPAQSPRRRLAAQLAETIR
                     NWIGRRIVAATGRTVQPDDILVLVQKRNVFFDALIRELRQRNVPVAGADRLKLAENIA
                     VQDLMALAAFCLMPEDDHARACVLKSPILARPLGEEQLMAIAIGRGTQTLWQTLSGSA
                     DPACRAAAAELGRLIAIAPSSRPYDFLAGLLVDSRMRFLSRLGSEANDALDALLGQAM
                     RFEEDHPATLAGFANWFAAGETEIKRNMEQASGEVRIMTVHGAKGLEAPIVILADTAT
                     APDTRQQPPLLLVEAAGRDVKLPLWPLTGLPDSVAITALKSRASELQAEEYRRLLYVA
                     MTRARDELYICGYTGRQAVKSDCWYELAVQGLGPIMREVEDGIRRLGPDPVFGPAR"
BASE COUNT          559 a         1303 c         1242 g          576 t
ORIGIN      
        1 gggtcaggag gcccgcaccg gcaggtccag cagctggcca cgcctggcgc gggcgctcaa
       61 cgaaccggcc agcgtgaggc cctatgccaa gccgcggccg cgcccgccgc tcgccgcccg
      121 gccccggcag ctcagcgtca cacgcatcga gaccttgatc cgcgatccct atgccatcta
      181 cgcccgccat gtgctgaagc ttcagccggt cgagccaatc gtcgcgcgcg ccgatccggc
      241 gcggcgcggc atggtcatcc acgaggcgat cagcgacttc ctgcaggcct atccggatgc
      301 gctgccgccc gatgccctgg ccgaactcct gcgcctgggc catgcgcatt tcgcagccgt
      361 gctcggcgat cccgccgtct tcagcttctg gtggccgcgt ttcgaacgca tcgccgactg
      421 gctggtcgaa caggagcggc agcagcgccc cggcatcgcg catatccatg ccgagatcga
      481 aggccgcatc gcgctttcga ttgccgatgc cgcctttcat ctcacctgcc gcgccgaccg
      541 agtcgacgtg ctgaaggacg gcacggcgcg catcatcgac tacaagaccg gtgccgtgcc
      601 ctcggcacgc gaggtgcggg cgggactggc gccgcagctc accctgcagg cggcgatcct
      661 cgcgcgcggc ggcttcacgg ccgtggcgcc catggaggta tccgcggtgt cctatgtgaa
      721 gctcagcggt ggcgatcccg ccggcgaaga caagggtccg gatctcggcg cggatctccc
      781 gcaggtggtc gcacagcatg tcgcggggct ggtgaagctg atcgccgcct atcgcaacgc
      841 cgaccagccc tatctgccgc gcgccatgat gaagcacgag gacgatgcga gcgactacga
      901 ccatctctcg cgctatcgcg aatgggcgct tgcgggagag gccaggtgac cgcgcagccc
      961 accgccagcg accggcagcg cgaggcctcc gatccgcgca gttcggcctg ggtcaatgcc
     1021 aatgccggat cgggcaagac gcgggtgctg gtggaccggg tgatccgcct gctgctcgac
     1081 ggcgcggaac cttcgaagat catgtgcctc accttcacca aggcggcagc ggccgaaatg
     1141 tcgaaccgcc ttttcgagcg cctcagcgcc tgggtggtgc tgccggagcc tgagctgcgt
     1201 gcgcagctgg ccgagcttgg cgtcgacgat ggcgaccccg ccacgctgcg ccgtgcccgc
     1261 cggctcttca cccgcgcgct ggaaacgcca ggcggcctca agatccagac catccatgcc
     1321 ttctgcgaac gcgtgctgca ggctttcccg gtggaagccg gcgtggtgcc acgctttgcc
     1381 gtgctggacg agcgtggcca gcgcgatctc ctgctggccg cgcggggcga ggtcctggat
     1441 gaggcgctga agcagccggc gggcgatctc ggcctggcgg tcgccgatgt cgtgcggcgg
     1501 gtgcagcccg atggcttcga tgccctgatg cagcatctcc tctcgcggcg cgccgagctc
     1561 ttcgggcttc tcgccacggc ggacggcgtc gatgccgtga tgacggtgct gggggcgcat
     1621 ctcgggatcg atccggccga gaccgcgcag caggtaaagg caggtctggc gcttgatctg
     1681 ccgcgctacc gcgacattgc cgatgcgctt gccaccggca gcaccgccga tcgcaagagc
     1741 ctcgatcaac tggcacccgt gctgctgcgg cccacgccaa acctgctcga tctccagacc
     1801 ttctatctca cgcaaaaggg cgaagcgcgt tccaggatgg ccaccaacgc cgtcctcgca
     1861 agattgccct ggcttgccgg tttcgccgcg ggcgaacagc agcgcctgct tgccgcgctg
     1921 ggccgccttg ccgatctcga gcgccttgcg gccacgcgcg cgctgctgat ccttgccgga
     1981 gccgtcatgc ggcgcttcga ggcggccaag cgccggcaag gtgcctatga cttcgatgac
     2041 ctcatcatcc gcaccgccga gctgctggcc acccgcagcg atgcgcaatg ggtgctcttc
     2101 aagctcgacg gcggcatcga gcatctgctg gtcgacgagg cgcaggacac atcgccggcg
     2161 caatggcgca tcgtcaccgc gctcacccag gaattcttcg ccggcgccgg gcgccatggc
     2221 gagaagcgcc gcaccatctt cgcggtgggc gaccgcaagc agtcgatcta cagtttccag
     2281 ggtgccgatc ccgacatctt cgccgaggtg cagatggcct tcgcacagcg ccttggcgaa
     2341 gcgggccagc gcctgcgcga tgtcgacttc acggtctcct tccgctccgc gcccgagatc
     2401 ctgcaggccg tcgacaaggt tttcgcgccc gatgccgtgg cgcggcgcgg actcgatggc
     2461 gccgccacgc gcgactgggt gcatgaatcg aaccgccgcg ccatcagggg catggtcgag
     2521 atctggccgc tggtcgcgcc gctcgacaag gaggatggcg atgtctggac cgcgccggtc
     2581 gatcgcgagc cggcgcagtc gccgcggcgc cggctggcgg cccagctggc cgagaccatc
     2641 cggaactgga taggcaggcg catcgtcgcc gccaccggcc ggacggtgca gccggatgac
     2701 atcctggtcc tggtgcagaa gcgcaacgtg ttcttcgacg cgctgatccg cgaactccgc
     2761 cagcgcaatg tgccggtggc cggcgccgac cgtctcaagc tggccgagaa catcgcggtc
     2821 caggatctca tggccctggc ggccttctgc ctcatgccgg aggatgacca tgcgcgggcc
     2881 tgcgtgctga agagtcccat cctggcccgg ccgctcggcg aagagcagct gatggcgatc
     2941 gccattggcc gcggaacgca gacgctgtgg cagacgcttt cgggcagcgc agatcccgcc
     3001 tgccgtgccg ccgcggcgga actcggccgg ctcattgcca tcgccccctc cagccgcccc
     3061 tatgacttcc tggccggact cctggtcgac tcgcgcatgc gcttcctgtc gcggctgggc
     3121 agcgaggcca atgacgcgct ggacgcgctg ctcggacagg ccatgcgctt cgaggaagat
     3181 catccggcca cgctggcggg cttcgccaac tggttcgcgg cgggcgagac cgaaatcaag
     3241 cgcaacatgg aacaggcctc gggcgaggtg cgtatcatga cggtgcacgg cgccaagggc
     3301 ctggaagcac ccatcgtcat ccttgccgac acggcgacgg caccggacac ccggcagcag
     3361 ccaccgctcc tgctggtcga ggcggcgggc cgcgacgtga agcttcccct gtggccgctg
     3421 acgggactgc cggactccgt ggctatcact gcactgaaga gccgggccag cgaactgcag
     3481 gccgaggagt atcgccgctt gctctatgtt gccatgacgc gggcgcgcga cgagctctat
     3541 atctgcggct acaccggccg gcaggccgtc aagtccgact gctggtacga gcttgcggtc
     3601 cagggcctcg ggccgatcat gcgcgaggtc gaagacggca tccggcgcct cggcccggat
     3661 ccggtcttcg gtccggcgcg
//
LOCUS       DBNGVR010000259         3871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5200523, whole genome shotgun sequence.
ACCESSION   DBNGVR010000259 DBNGVR010000000
VERSION     DBNGVR010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5200523"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16A0_05540"
     CDS             <1..189
                     /locus_tag="AC16A0_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein"
                     /protein_id="HIR3104828.1"
                     /translation="NVADAMRFAFEVLKLVIEPGGAVALAAVLSGKLPTRGRNIGVII
                     SGGNADASLFARVLRGEI"
     gene            complement(186..755)
                     /locus_tag="AC16A0_05545"
     CDS             complement(186..755)
                     /locus_tag="AC16A0_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104829.1"
                     /translation="MSTALSPESFEAIESAVMETERGRWFLAEYAKRLRSRETTQLLD
                     HMRRLEATVSGNHDELIARLGEALTRSPQPGTAVPPVPELAPRHMKYFKKDEDIFEPA
                     PQATIAAVKPPALPDEKRGARLTITRIGAAPEPADVEAAQEQPRQPEAVAEEPPQAAA
                     PPSRRIVIVRHKPGEEISVPVQEDLARTG"
     gene            868..2028
                     /locus_tag="AC16A0_05550"
     CDS             868..2028
                     /locus_tag="AC16A0_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013473.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3104830.1"
                     /translation="MSSRSFFVRFSAFNAFLFLGTGIQMPFLPLWLRSEGLSDSQISL
                     IVAAMVAIRIFAMPLGTYLADLTGNRRRVIMLSTLASFICYGLLYFVDGFLGLLVMAV
                     LAAALFAPVGPLIEVFAIEGSAHYGIDYGRIRLWASLSFLSGSLLSGLLLEFVPVSAV
                     ILLIAGAQGIGALATLLLPPDHGIVKPKTRQIRLSAVLVCVLAASFLIFAAASSIGQA
                     SHGLFYAMGSVYFSDLGYSEFTIGKLWAVAVISEIVMFAFSNRIHKRFGSVNLIALGV
                     SCGALRWGLMALEPPLAIMYLAQTLHAGSFGLTHLGTMHYIRETVPDGMRNTVKGLYS
                     ALSGGILLSASMWAAGPLYGALGGQAYWVMAACAAIALVLALILARISPRAS"
     gene            complement(2009..2986)
                     /gene="dgcA"
                     /locus_tag="AC16A0_05555"
     CDS             complement(2009..2986)
                     /gene="dgcA"
                     /locus_tag="AC16A0_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HIR3104831.1"
                     /translation="MRLTVAAESWPIAGSFTISRGSKTQADVVVVTVNDGNHTGRGEC
                     VPYPRYAETVPGVIAALEDARAAIEAGIDRADVPKCVAPHAARNALDCALWDLEAKRA
                     DRPVWQLAGLHEPQALVTAYTLSLDTPEAMAEAAARAAHRPLLKLKLGRDGDADRLRR
                     IRAAAPKSRLIVDANEGWSPDTLAVMLKTCADVGVELVEQPLPAGNDEALRHVERPVP
                     VCADESAHDRSGLELLVGKYDAINVKLDKTGGLTEALALARDAQSIGQRIMVGCMLAT
                     SLAMAPAMLVAQYADVVDLDGPLLLKQDRNPGIRFEGSLMYPAPSSLWG"
     gene            complement(2991..>3871)
                     /locus_tag="AC16A0_05560"
     CDS             complement(2991..>3871)
                     /locus_tag="AC16A0_05560"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase DgcN family protein"
                     /protein_id="HIR3104832.1"
                     /translation="ADTGLPDISIADAAAKGAKTMVVGVANAGGVLPDHWISEIVAAL
                     DAGLDVATGLHKRLGSIPEIAAAAARHGRKLHDVRFSDISFATGKGTKRPGRRLLTVG
                     TDCSVGKKYTALAMEREMRRLGLNAEFRATGQTGVLISGRGIAIDAVVADFIAGAAEW
                     ISPDNEPDHWDVIEGQGSLFHPSFSGVTLGLLHGAQPDAFIVCHEPTRRTMRGVQHGL
                     PSIAQVIERTVLEGQLTNPAITCIGIAINTEHLSEPEALACLAETGKAHDLPCVDPIR
                     TGVTPLVEDLRHRFGV"
BASE COUNT          601 a         1245 c         1244 g          781 t
ORIGIN      
        1 aacgtggccg atgccatgcg ctttgctttc gaagtgctga agctggtgat cgagcccggc
       61 ggcgccgtgg ccctcgcggc cgtcctgtct ggaaagctgc cgacacgcgg ccgcaacatc
      121 ggcgtgatca tttctggcgg caatgcggat gcctcgctct tcgccagagt gcttcgcggc
      181 gagatctagc ccgtacgggc aaggtcctcc tgcaccggaa cgctgatctc ttcaccgggc
      241 ttgtggcgca cgatgacgat cctgcggctt gggggagccg cggcctgcgg cggctcttcg
      301 gcgaccgctt caggctggcg cggctgctcc tgagcggcct cgacgtcggc tggttcgggt
      361 gccgcaccga tacgggtgat ggtcagtctg gcgccacgct tctcgtcggg gagggccggt
      421 ggcttcacgg ccgcaatggt ggcctgcggc gcaggctcga agatgtcctc gtccttcttg
      481 aagtatttca tgtggcgagg tgccagttcg ggaacaggtg gcacagccgt gcctggctgc
      541 gggctgcgcg tcagtgcttc gccgagacgg gcgatcagtt cgtcgtgatt gccagaaacc
      601 gttgcctcca ggcggcgcat gtgatccaga agctgcgtgg tctcgcgcga tctgaggcgc
      661 ttggcgtatt cagccaggaa ccagcggccg cgctccgttt ccattacggc agactcgatg
      721 gcctcgaagg actcaggcga caacgcggtc gacatacgct aactccacac acaaacacct
      781 ctgcagaact cgcagatgac ccggacgtca attatgttag ggaatggtga atctttagtt
      841 taaggccgcg ctcgatcagt caagatcatg tcttcacgca gttttttcgt ccggttttcg
      901 gccttcaacg ctttcctctt cctggggact ggcatccaga tgccgttcct gccgctgtgg
      961 ctgagaagcg agggactgtc cgattcccag atttccctga tcgtggcggc gatggtggcc
     1021 atccgcatct tcgccatgcc gctgggcacc tacctggccg acctgaccgg caaccgccgc
     1081 cgcgtcatca tgctgtcgac cctcgcatcc ttcatctgtt acgggttgct ctacttcgtt
     1141 gacggctttc tgggcctgct ggtcatggcc gtgcttgcgg cggcactctt tgcgccagtc
     1201 gggccgctga tcgaggtctt cgcgatcgag ggaagtgccc actacggtat cgactatggc
     1261 cgcatccggc tctgggcatc actgagcttt ctctccggca gcctgctgtc cggcctgttg
     1321 ctcgaattcg taccggtcag tgctgtcatt ctgctcattg ccggtgctca ggggattggt
     1381 gcgctcgcga cgctgttgct tccgcccgat catggcatcg tcaagcccaa gaccaggcag
     1441 atccgccttt cggctgtcct cgtctgcgtc ctggctgcaa gttttctgat cttcgcggca
     1501 gcatcaagca tcggacaggc cagtcacgga ctcttctatg ccatgggttc ggtctatttc
     1561 agcgatctgg gctacagcga gttcaccatc ggcaagcttt gggcggtggc cgtcatctcc
     1621 gaaatcgtga tgtttgcctt ctccaaccgc attcacaagc gtttcggctc ggtcaacctg
     1681 atcgcccttg gagtgagctg tggcgcgttg cgctggggcc tgatggcgct ggaaccgccg
     1741 ctcgccatca tgtacctggc gcagacgctg catgccggat cattcggact cacccacctg
     1801 ggcaccatgc actacatccg tgaaacagta ccggacggca tgcggaatac cgttaagggc
     1861 ctctattccg cactctccgg cggcatcctg ctgtccgctt ccatgtgggc ggctgggccc
     1921 ctctacggcg cccttggcgg gcaggcctac tgggtgatgg cagcctgtgc ggcaattgcc
     1981 cttgtgctgg cgctgatcct cgcccgcatc agccccagag cgagctaggc gccggataca
     2041 tcaggcttcc ctcgaaacgg attcccgggt tgcgatcctg tttcagcagc aacggcccgt
     2101 cgagatcgac cacatcggcg tattgtgcca ccagcatggc gggcgccatg gccagcgagg
     2161 tcgcaagcat gcaacccacc atgatgcgct ggcctatgct ctgtgcgtcc cgtgcgagcg
     2221 ccagcgcctc cgtcaggccg ccggtcttgt cgagcttcac gttgatggcg tcatactttc
     2281 ccaccagcag ctccaggccg ctgcggtcat gcgcggactc atcggcacag acgggaaccg
     2341 gcctttccac atgacgcaag gcctcgtcat tgccggcggg cagcggctgc tcgacgagtt
     2401 ccacccccac gtcggcacag gtcttgagca tcaccgcaag ggtgtcaggc gaccagccct
     2461 cgttggcgtc cacaatgaga cggctcttcg gtgccgccgc gcgtatgcgc cgcagccggt
     2521 cggcatctcc gtctcggccg agcttcagct tcagaagcgg acgatgcgcg gcgcgggccg
     2581 cagcctccgc catggcttcg ggcgtgtcga gactgagcgt ataggcggtc acaagtgcct
     2641 gcggctcatg cagcccggca agctgccaca cgggccggtc cgcgcgcttt gcctcgaggt
     2701 cccagagcgc gcaatcgagc gcgttgcggg cggcatgcgg tgccacgcac ttcggcacgt
     2761 cggcgcggtc aatgccggct tcaatggcgg cgcgcgcatc ttccagcgcg gcgatgacgc
     2821 caggaacggt ttccgcatac cgcggatagg gaacgcattc gccgcgtccc gtgtggttgc
     2881 catcgttgac cgtcaccacc acgacgtccg cctgggtctt gctgccgcga gaaatggtga
     2941 agcttccggc gatgggccag ctttcggcgg caacggtcag gcgcatgtgg tcagacccca
     3001 aaacggtggc gcaggtcctc gaccagcggc gtgacgcctg tccggatcgg atcgacgcag
     3061 ggcaggtcat gggccttgcc ggtttcggcg aggcaggcga gcgcttccgg ttcggacagg
     3121 tgctcggtat tgatggcgat gccgatgcaa gtgatggccg ggttggtgag ctgtccttcg
     3181 agaaccgtgc gctcgatcac ctgcgcgatg ctcggaagcc cgtgttgcac gccgcgcatc
     3241 gtccttcggg tcggttcatg gcagacgatg aaggcgtctg gctgggcacc gtgcagaaga
     3301 cccagcgtga cgcccgagaa ggatggatgg aacagcgagc cctgaccctc gatcacatcc
     3361 caatggtcgg gttcgttgtc cggcgagatc cactcggcag cgcctgcgat gaaatccgcc
     3421 acaacggcat cgatggcgat gccgcgtccc gaaatgagca cgccagtctg gccggtggcg
     3481 cggaattcgg cgttgaggcc gagcctgcgc atctcgcgct ccatggccag ggcggtatac
     3541 tttttgccga ccgagcagtc cgttccgacg gtaagcaggc gccggccggg gcgcttcgtg
     3601 cctttgccgg ttgcgaagga gatgtccgag aagcgcacgt catgcagctt gcggccatgc
     3661 cgcgccgccg cggccgcgat ttcggggatg gagccgagcc gcttgtgcag gccggtcgca
     3721 acatcgaggc cggcgtcgag tgcggcgacg atctcactga tccagtgatc gggcagcacg
     3781 ccgccggcgt tggcgacgcc aaccaccatg gtcttcgccc ccttggcggc cgcatcggcg
     3841 atgctgatgt cgggcagacc ggtatccgcc t
//
LOCUS       DBNGVR010000260         2389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3827536, whole genome shotgun sequence.
ACCESSION   DBNGVR010000260 DBNGVR010000000
VERSION     DBNGVR010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3827536"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="AC16A0_05565"
     CDS             complement(<1..723)
                     /locus_tag="AC16A0_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3104833.1"
                     /translation="MTHSGTLALKRLTLTDFRNYAGLRLDVDGPAIALTGPNGAGKTN
                     LLEAISLLAPGRGLRGAAFEDLARQGGSGTWAIAVEAETPEGPVSLGTGWSGQGEGDS
                     PAQGRLVLVDGHVQKSSGALGQFMRVLWLTPAMDRLFAGPASDRRRFMDRLVAAFDPE
                     HASRLLVFEKVMRERNLLLEDPRADGTWLGSLEAHRAEAAVAIAAARLTALDALRFHI
                     GETRETSRFPWSEISLDGEIEAL"
     gene            complement(734..1846)
                     /gene="dnaN"
                     /locus_tag="AC16A0_05570"
     CDS             complement(734..1846)
                     /gene="dnaN"
                     /locus_tag="AC16A0_05570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3104834.1"
                     /translation="MRVTIERSAFLRALNHVQSVVERRNTIPILSNVLLQADGAALKL
                     TATDLDIEIVESVPAMIAKAGAATVPAHMLYDIVRKLPDGAQLELDQSGDSNRMAIFA
                     GRSRFNLQALPPEDFPDISAGDFSNRFSLPAGTLKGLIEKTRFAISTEETRYYLNGIY
                     FHEVAAGNLLRAVATDGHRLAQAQIARPEGAKGMPGVIVPRKTVLEVVKLLEGAEADV
                     EVSLSAAKIRFAAGDLVLTSKLIDGTFPDYERVIPRSNDKTMEIDTRTFASAVDRVST
                     ISMEKGRAVKLHISADKLMLSVNNPDSGSAEEELVCSYGADPIEIGFNSRYLMDIAGQ
                     IKSDSVVFSLADAGSPTIVRDPGDEQALYVLMPMRV"
     gene            complement(1944..>2389)
                     /locus_tag="AC16A0_05575"
     CDS             complement(1944..>2389)
                     /locus_tag="AC16A0_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019903.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3104835.1"
                     /translation="EFIANRMTGGGRELEGALNRVMAYQQFNQLPVTLDLAAMVLRDM
                     SANPDQSRVKIEDILKVVGRHFNVARTDLLSPRRARTVVVPRQIGMYLAKKLTSRSLP
                     EIGRRFGGRDHSTVLHAVRKIEDQIKTDDKLAREVAMLIRLVEQG"
BASE COUNT          420 a          758 c          797 g          414 t
ORIGIN      
        1 cagggcttcg atctcgccgt caaggctgat ttcgctccac ggaaaacggc tggtttcccg
       61 ggtttcgcca atgtggaagc ggagcgcatc aagggccgtc agccgggccg ccgcgatggc
      121 caccgcggct tccgccctgt gcgcttcgag gctgccaagc caggttccat ccgcgcgcgg
      181 atcctccagc agaaggttgc gctcgcgcat gaccttttcg aaaaccagca ggcgggaggc
      241 gtgctcggga tcgaaggccg ccaccagccg gtccatgaag cggcgccggt cggaggccgg
      301 gccggcaaag agccggtcca tggccggcgt gagccacagc acccgcatga actggcccag
      361 ggcgccggaa ctcttctgga catgcccgtc gaccagaacc agccggccct gggcgggact
      421 gtcgccttcg ccctggccgc tccagccggt gccgagcgaa acgggccctt ccggggtttc
      481 ggcctccact gcaatggccc aggtgccgct gccgccctgc cgggcaaggt cttcgaaggc
      541 cgcgccgcgc aggccgcggc ccggcgccag cagcgagatg gcctccagca gattggtctt
      601 gcccgcacca ttggggcccg tcagggcaat ggccggcccg tcaacatcca gcctcaggcc
      661 ggcatagttg cggaagtcgg tgagcgtgag gcgtttgagc gccagcgtgc cggaatgggt
      721 cacggccgcc cggctacacc cgcatgggca tcagcacata gagggcctgc tcgtcgcccg
      781 ggtcgcgcac gatggtgggc gaacccgcat cggcgaggct gaagaccacg gagtccgatt
      841 tgatctggcc tgcgatgtcc atcaagtagc gcgagttgaa cccgatctcg atcgggtcag
      901 cgccatagct gcagacgagt tcttcctcgg ccgagccgga gtcggggttg ttgaccgaga
      961 gcatcagctt gtcagccgag atatgcagct tcacggcccg gcccttctcc atcgaaatgg
     1021 tcgacacgcg atcgaccgcc gaggcaaagg tccgggtgtc gatttccatc gtcttgtcat
     1081 tgctgcgtgg aatgacgcgt tcgtagtccg ggaaggttcc atcgatgagc ttcgacgtca
     1141 gcaccagatc tccggcagcg aaacggatct tcgcagccga gagcgagacc tcgacatccg
     1201 cctcggcacc ttcgaggagc ttcaccactt ccagcaccgt cttgcgcggc acgatcacgc
     1261 cgggcattcc cttggcccct tcgggccgtg cgatctgggc ctgggccagg cggtggccat
     1321 cggtcgccac ggcgcgcagc aggttgcccg ccgcgacttc gtgaaagtag atgccattca
     1381 gatagtagcg ggtttcctcg gtggagatcg cgaaacgggt cttctcgatg aggcccttca
     1441 gcgtgccagc cgggaggctg aagcggttgg agaaatcgcc ggcggaaatg tccggaaagt
     1501 cttctggcgg cagcgcctgc agattgaagc gcgagcggcc cgcgaagatg gccatgcggt
     1561 tgctgtcgcc gctctggtcg agctcgagct gggcgccatc gggcagcttg cggacgatgt
     1621 cgtagagcat gtgcgccggc acggtcgctg cgccggcctt ggcgatcatg gcgggcacgc
     1681 tttcgacgat ctcgatgtcg agatccgtag ccgtcagctt cagcgcggcg ccgtcggcct
     1741 gcaacagcac attggacagg atcgggatgg tgttgcggcg ttccaccacc gactgcacat
     1801 ggttcagggc ccgcagaaag gcagagcgct cgatcgttac acgcatgacc ggaaatccct
     1861 atgaaacaag gcccggcgcg ctggcgccgg gccggagaca ttggcgggaa aacaggccgg
     1921 attcaagcct cggatggaaa aaatcagccc tgctcgacga gtcggatcag cattgccact
     1981 tcgcgcgcca gcttgtcatc ggtcttgatc tggtcttcga tcttgcggac cgcgtgcagc
     2041 acggtcgaat gatcgcgccc tccgaaccgg cggccaattt ccggcagcga ccgcgatgtg
     2101 agtttcttgg ccaggtacat gccgatctgg cgtggcacca cgacggtccg cgcacggcga
     2161 ggtgacagga gatcggtgcg ggccacgttg aaatgacggc cgacgacctt gaggatgtcc
     2221 tcgatcttga cgcgcgactg atcgggattg gccgacatgt cgcgcaggac catcgcggcc
     2281 agatcgagcg tgaccggcaa ctggttgaat tgctgatagg ccatgacgcg attgagggcg
     2341 ccctcgagtt cgcgtccgcc accggtcatg cgattggcaa tgaactcca
//
LOCUS       DBNGVR010000261         1897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3434596, whole genome shotgun sequence.
ACCESSION   DBNGVR010000261 DBNGVR010000000
VERSION     DBNGVR010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3434596"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC16A0_05580"
     CDS             <1..270
                     /locus_tag="AC16A0_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HIR3104836.1"
                     /translation="KNNAKEAVSLLKKAQRTENDMPVLYQYLGMAYGKMGDIPRAELA
                     TAEFAMLRGDRKLALDKANAAQKLFKTGTPEWTRANDILTFASRK"
     gene            324..1073
                     /locus_tag="AC16A0_05585"
     CDS             324..1073
                     /locus_tag="AC16A0_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3104837.1"
                     /translation="MLKTLQAATLVFAVATAPALAGGFDEAQRKEIGDIVRDYLLEHP
                     EILLEVSKKLEEKQQAEETAKREDALKALAGDIFRMKGDHVAGNPEGDVTMVEFFDYN
                     CGWCKKGMPEVLAMVEEDKNLRLVLKEFPIFGGDSDYAAKAALASQKQGKYWEFHVAM
                     LGHEGKVTRETVDKIAAEVGLDVARLKTDMESPEVAETIRKNQELATALAIGGTPAFV
                     IDNQVVPGYLPKDGLMASVNKVREQGGCKVC"
     gene            1161..1610
                     /gene="aroQ"
                     /locus_tag="AC16A0_05590"
     CDS             1161..1610
                     /gene="aroQ"
                     /locus_tag="AC16A0_05590"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3104838.1"
                     /translation="MKPIYVLNGPNLNMLGQREPSVYGAETLPEIEKLCRARATELGL
                     TVDFRQSNIEGELVSWIQEARSKASGIALNAGAYTHTSVAIHDALRAADVPAVEVHLS
                     NVYKREAFRHHSFIAAAVNGVICGFGSHSYVLALEALASLVNINKKG"
     gene            1615..>1897
                     /locus_tag="AC16A0_05595"
     CDS             1615..>1897
                     /locus_tag="AC16A0_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3104839.1"
                     /translation="MPSKKKAPAAPAAASKSDPELDLINSLAEILNRTGLSEIELDRR
                     GTRLRVSKQVTVTAMAAPAVHHQAPVATATPAGSPAPAEVPARGKEADHP"
BASE COUNT          411 a          602 c          576 g          308 t
ORIGIN      
        1 aagaacaatg ccaaggaagc cgtcagcctc ctgaagaagg cccagcgaac cgaaaacgac
       61 atgccggtgc tgtatcagta tcttggcatg gcctatggca agatgggtga cattccccgc
      121 gccgaactgg caacggccga gtttgcaatg ctgcgcggcg accgcaagct ggccctcgac
      181 aaggccaatg ccgcgcagaa gctgttcaag accggcaccc cggaatggac tcgcgctaac
      241 gacattttga cttttgccag ccgtaagtga cgtagacgcc acagccgttt ccgggcagtg
      301 tcggcacaaa acagaggatc aatatgctca agacccttca ggccgccact ctcgtgttcg
      361 ccgtcgccac cgcgcccgcg ctggccggcg gatttgacga agcccagcgc aaggaaatcg
      421 gcgatatcgt ccgcgactac ctgcttgaac accccgagat cctgctcgag gtcagcaaga
      481 agctcgagga gaagcaacag gccgaggaaa ccgccaagcg cgaggatgcg ctgaaggcat
      541 tggcgggcga tatcttccgc atgaagggcg atcacgtcgc gggcaatccc gagggcgacg
      601 tcacgatggt cgagttcttc gactacaatt gcggctggtg caagaagggc atgcccgagg
      661 tcctggccat ggtcgaggag gacaagaacc tccgcctggt gctgaaggag tttccgattt
      721 tcggcggcga ctcggattat gcggccaagg cggcgctcgc ctcgcagaag cagggaaagt
      781 actgggagtt ccatgtcgcc atgctgggcc acgagggcaa ggtcacgcgt gagacggtcg
      841 acaagatcgc agccgaggtg ggcctcgatg tcgcaagact gaagaccgac atggaaagcc
      901 cggaggtcgc ggaaaccatc cgcaagaacc aggagcttgc gacggccctc gccatcggcg
      961 gcaccccggc cttcgtgatc gacaaccagg tggttccggg ctatctgccc aaggatggcc
     1021 tcatggcgtc ggtcaacaag gtacgcgaac agggcggctg caaggtctgc tgattgtcca
     1081 ccggtcctgc cggcgacgct tccaacctct caccggaccg cctataagcg gttgtccaac
     1141 ctcggaatcg tggcgcagga atgaagccca tctacgtgct gaacggcccg aacctcaaca
     1201 tgctcggcca gcgtgaaccc tctgtctatg gcgcagagac cctgcccgag atcgagaagc
     1261 tttgccgtgc aagggccact gaactcgggc tcaccgtcga cttccgccag tccaacatcg
     1321 aaggcgaact ggtctcgtgg atccaggagg cgcgcagcaa ggcctcgggg atcgccctca
     1381 atgccggtgc ctacacccat acgtcggttg caattcacga cgcgctccgc gccgccgacg
     1441 ttcccgcagt cgaggttcat ctctcgaacg tctacaagcg cgaggcgttc cggcaccaca
     1501 gtttcatcgc ggcagccgtc aatggcgtca tctgtggttt tggaagccac agctatgtcc
     1561 ttgccttgga ggccctcgca tccctcgtca acatcaacaa gaagggttga gcaaatgccc
     1621 tcgaagaaaa aagccccggc ggcacccgct gccgcatcca agtccgaccc cgaactggac
     1681 ctgatcaaca gcctcgcgga gatcctcaac aggaccggct tgtcggagat cgaactggat
     1741 cgcaggggaa cccggttgcg cgtctcgaag caggtcacgg ttaccgccat ggcagcgcct
     1801 gccgtgcatc atcaggcgcc cgtcgccacg gccacgccag ccggttcgcc ggcaccggca
     1861 gaagtcccgg cgaggggcaa ggaggccgat catcccg
//
LOCUS       DBNGVR010000262         5810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5007415, whole genome shotgun sequence.
ACCESSION   DBNGVR010000262 DBNGVR010000000
VERSION     DBNGVR010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5007415"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="AC16A0_05600"
     CDS             <1..386
                     /locus_tag="AC16A0_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3104840.1"
                     /translation="VGRSLNPALDIGQVEGGFVQGMGWLTTEELVFDSQGRLRTHAPS
                     TYKIPCASDVPADFRVRLYESRGNKEETIYRSKAVGEPPLMLGISVWTAVFDAIARTN
                     PGQIPRLDAPATPEAILRALKGGGA"
     gene            383..1234
                     /gene="xdhC"
                     /locus_tag="AC16A0_05605"
     CDS             383..1234
                     /gene="xdhC"
                     /locus_tag="AC16A0_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HIR3104841.1"
                     /translation="MKIWGHICDALERHPACARVTVVATRGSAPREAGAAMAVTPEGY
                     HGTIGGGALEWRAIATAQAMLSRGAGSRRTSHALGPELGQCCGGHVDLLTEVFDRSDL
                     PDIRRRMADAGEARRRVYLFGAGHVGRALVLALAPLPFEVMWIDPRVSAFPAVMPSNA
                     TPLAPEDAPAVLAEATGGSLAYIMSHSHALDLAIADAALRNASVARVGLIGSATKRAR
                     FVKRLREAGVAEDRINGLICPIGVEGIGSKEPAMIALATAAQIASLHEQLQSAAQASP
                     DVQRSRA"
     gene            1231..2778
                     /locus_tag="AC16A0_05610"
     CDS             1231..2778
                     /locus_tag="AC16A0_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104842.1"
                     /translation="MNRGPEYLLEALHVVKVFGTLRANDDVTLKVKPGEIHAFLGENG
                     AGKSTLVKMIYGALQPTSGEFRWMGQPVTIASPAAARKLGIGMVFQHFSLFEALTVAE
                     NVALAMPGDFDMAKLSEKIARVSKEYGLPLAPTATVADLSVGERQRIEIVRCLLQEPK
                     LLIMDEPTAVLTPQEADQLFVTLTRLAKEGCAVIYISHRLEEVKRLCHNVTILRHGKV
                     VANVDPAKENAASLAKLMVGGDIHVVRGNRKHAAGEVRLKLDRLSLDPDGPFAVELKE
                     ISLDVRAGEVVAIAGVAGNGQSEFFDAVSGERILHGDSMLQIDGKAMGRAGVNARRKA
                     GAAFVPEERLGHGAVPAFSLSENVILTRHASDKSLIRSGLVQRGDASGISHRVIKDFD
                     VRKGKPDPEARSLSGGNLQKFVIGRELDRKPGLLVVNQPTWGVDAGASATIRQAIIDL
                     SRQGSAVLVISQDLDEIFEIADRIAVISRGQLSEAYPADKLTAEKIGLLMAGEHEIKE
                     VRHAAGA"
     gene            2762..3841
                     /locus_tag="AC16A0_05615"
     CDS             2762..3841
                     /locus_tag="AC16A0_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104843.1"
                     /translation="MRLVLEKRAEQSVRMAILSPFIALALTVIAGGIIFALRGLNPFE
                     ALYVYFIEPLTQDWAREQLIVKAAPLILIGSGLAVCYTANVWNIGAEGQFVIGALLAG
                     MIPVYFPAWQSPDVMVVMLALGIIGGMAWGAIPAFLKIRFSANEILTSLMLVYVAQYL
                     FDWLVRGPWRDPQGFNFPKTVNFAGWQLLPKWGEIHLGALFALIAAIVLAVVMWRMLK
                     GFELRVLGSAPRAGRFAGFSTERATLFCFLLSGGLAGLAGASDVMGVSGQLQTPYAPN
                     YGFVAIIVAFLCRLNPIGVIFAGLLLALSYIGGESAQVSLGISDKIAKVFQGILLFFI
                     LACDTLILYRIRIESGRAAQTRTAG"
     gene            3846..4721
                     /locus_tag="AC16A0_05620"
     CDS             3846..4721
                     /locus_tag="AC16A0_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104844.1"
                     /translation="MDMMIAVVLTIMTAATPLLLAALGELVVERSGVLNLGVEGMMVM
                     GAVCGFGAALMTGSPWLGLLASIAAGMLMSLLFGFLTLSLVSNQVATGLALTLLGLGL
                     SGQIGESFVGQPGVKLPNYDGFDPLIPFAILLAWGVSWFLFKTKAGLIVRAVGNSHAS
                     AHAMGYHVIRVRYLCVLFGGACAGLAGGYLSLVYTPQWIENMTSGRGWIALALVVFAS
                     WRPWRTVAGAYLFGAISILAFTVQTFAWGIPSQFLSMLPYLVTIIALVVISGNRMLTR
                     ANTPACLGQPFVPDR"
     gene            4748..>5810
                     /locus_tag="AC16A0_05625"
     CDS             4748..>5810
                     /locus_tag="AC16A0_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3104845.1"
                     /translation="MKKLMMSAAAFAMALGVAAASADAEAKTKACWVYVGSIGDFGWT
                     YEHNQGRLAVEKELGDKVETAYVENVPEADSERAIERLARDGCNIIFTTSFGFMEPTL
                     KVAAKFPDVKFEHATGYKTAENVTTYNAKFHEGRYVIGQIAAKMSKSGTAGYIVSFPI
                     PEVIAGINAFMLGAQSVNPDFKVKIVWANTWYDPGKEADAAKVLFSQGADIIVQHTDS
                     TAPLQVAEEQGKQGFGQASDMIKFAPKAQLTAIVDHWAPYYVRRVNDVINGTWKSQSS
                     WDGMAEGTVQIAPFTNMPDDVAKMASETAEAIKTGKLHPFTGPITKQDGTVVGEAGKP
                     LPDGDILGMNWYVKGVDDKL"
BASE COUNT          949 a         1928 c         1857 g         1076 t
ORIGIN      
        1 acgtcgggcg ctcgctcaat cccgcgctcg acatcggcca ggtggagggc ggcttcgtgc
       61 agggcatggg ttggctcacc accgaagagt tggtcttcga ttcgcagggg cggcttcgta
      121 cccacgcgcc ttccacctac aagatcccct gcgcctccga tgtgcctgcc gattttcgcg
      181 tcaggcttta cgagtcccgg ggcaacaagg aggagaccat ctaccggtcc aaggccgtgg
      241 gagagcctcc gctgatgctg ggcatttccg tctggactgc cgtttttgac gccattgccc
      301 gcacaaatcc cgggcagatt ccaaggcttg acgcgcctgc gacacctgag gccatattgc
      361 gggccttgaa aggcggcggg gcatgaagat ctgggggcac atctgcgacg cgctggagcg
      421 gcatccggcc tgtgcccgcg tcaccgtggt tgccacccgc ggctcggccc cgcgcgaagc
      481 aggtgccgcc atggccgtga cgccggaagg ctatcacggc acgatcggcg gcggcgcgct
      541 cgagtggcgc gccatcgcca ccgcccaggc catgctgtcc cgcggtgccg gatcgcgccg
      601 caccagccat gcgctcggtc ccgagcttgg ccaatgctgc ggtggccatg tcgatctcct
      661 gaccgaggtc ttcgatcgca gcgacttgcc ggacattcgc cgccgcatgg cggatgccgg
      721 cgaagcgcgc cgccgcgtct atcttttcgg tgcgggccat gtcgggcggg ccctggtcct
      781 ggcgctggcg ccattgccct tcgaggtgat gtggatcgac ccgcgcgtgt cggcctttcc
      841 tgcggtgatg ccatccaatg ccacgccgct cgcgccggaa gatgcacccg ccgtcctggc
      901 cgaagcgacg ggtggcagcc tcgcctacat catgtcgcat agccacgcgc tcgaccttgc
      961 cattgccgat gcggcgctgc gcaacgcctc tgttgcccgc gtcggcctca tcggcagcgc
     1021 caccaagcgg gcgcgtttcg tgaagcgtct gcgcgaggca ggcgttgccg aagaccgcat
     1081 aaatggcctg atctgcccga tcggtgtcga aggcataggc tcgaaggaac cggcaatgat
     1141 cgcgctggcc acggcggcgc agatcgcatc gctgcacgaa cagctgcagt ctgccgcgca
     1201 ggcctcgccc gacgtgcaga ggtcgcgggc atgaacaggg gtccggaata tctgctcgaa
     1261 gcgctgcatg tcgtaaaggt cttcggcacc ttgcgtgcca atgacgacgt cacgctcaag
     1321 gtgaagcccg gcgagatcca tgccttcctt ggcgagaacg gggcgggcaa gtccaccctg
     1381 gtcaagatga tctacggcgc gctgcagccc acctccggcg aattccgctg gatgggccag
     1441 ccggtgacca tcgccagtcc ggccgctgcc cggaagcttg ggatcggcat ggtgttccag
     1501 catttctcgc tcttcgaggc gctcaccgtg gccgagaacg tcgcgctggc aatgccaggc
     1561 gacttcgaca tggcgaagct ttccgagaag atcgcccgcg tctcgaagga gtatgggttg
     1621 ccgctggcgc ccaccgccac tgtcgccgat ctctcggttg gcgagcgcca gcgcatcgag
     1681 atcgtgcgat gcctgctgca ggaacccaag ctcctgatca tggacgagcc gacggcggtg
     1741 ctgacgccgc aggaggccga tcagctcttc gtgacgctga cccggctggc caaggaaggc
     1801 tgcgccgtca tctacatcag tcatcgcctg gaagaggtga agcgtctctg ccacaacgtc
     1861 accatcctgc gccacggcaa ggtggtggcg aatgtcgatc cggccaagga aaatgccgcg
     1921 agcctcgcca agctcatggt gggtggtgac atccatgtcg tgcgcggcaa ccgcaagcat
     1981 gcggccggcg aggtgcggct gaagctcgac cgcctgtcgc tcgatcccga cggtcccttc
     2041 gccgtcgagc tgaaggaaat ctcgctcgac gtgagggccg gcgaagttgt ggccatcgcg
     2101 ggcgttgccg gcaacggcca gtcggagttc ttcgatgccg tctcgggcga gcgcatcctg
     2161 cacggcgact ccatgctgca gatcgacggc aaggccatgg gccgcgcagg cgtcaatgcg
     2221 cggcgcaagg cgggcgcggc cttcgtgcca gaggaacgcc tgggccatgg cgccgtaccg
     2281 gccttcagcc tgtcggagaa cgtgattctc acccgccatg cctcggacaa gtcactgatc
     2341 cgctcgggcc tcgtgcagcg cggcgatgcc tcgggcatca gtcaccgggt gatcaaggat
     2401 ttcgatgtgc gcaagggcaa gccggatccc gaggcgcggt cgctctcggg cggcaacctg
     2461 caaaaattcg tgatcggccg ggaactcgac cgcaagcccg gcctgctggt ggtgaaccag
     2521 ccgacctggg gcgtcgatgc cggcgcctcc gccaccatcc gccaggccat catcgacctg
     2581 tccaggcaag gctctgccgt gctggtcatc agccaggatc tcgacgagat cttcgagatc
     2641 gccgaccgca tcgccgtcat ctcgcgcggc cagctgtcgg aagcctatcc ggccgacaag
     2701 ctcaccgccg agaagatcgg cctgctcatg gcaggcgaac acgagatcaa agaggtgcgc
     2761 catgcggctg gtgcttgaga agcgcgccga acagtcggtg cgcatggcga tcctctcgcc
     2821 cttcattgcc ctggcgctca ccgtcatcgc cgggggcatc atcttcgcgc tgcgcggcct
     2881 caatcccttc gaggcgctct atgtctattt catcgaacct ctgactcagg attgggcgcg
     2941 cgagcagctg atcgtcaagg cagcacccct catcctcatc gggtccggcc ttgccgtctg
     3001 ctacaccgcc aatgtctgga acatcggtgc cgaaggacag ttcgttatcg gcgccctgct
     3061 ggctggcatg atcccggtct actttcccgc ctggcagtcg ccggacgtca tggtggtgat
     3121 gctggcgctg ggcatcatcg gtggcatggc ctggggcgcc ataccggcct ttctgaagat
     3181 caggttcagc gccaacgaga ttctcacctc cctgatgctg gtctatgtgg cgcagtatct
     3241 cttcgactgg ctggtgcgcg gtccctggcg cgacccgcag ggcttcaact tccccaagac
     3301 ggtgaacttc gccggctggc agcttctgcc gaagtggggt gaaatccacc tgggtgcgct
     3361 cttcgcgctg atcgccgcca tcgtcctggc agtcgtgatg tggcgcatgc tcaagggctt
     3421 cgaactgcgg gttcttggca gcgcgccgcg ggccggtcgc ttcgccggtt tctccaccga
     3481 gcgcgcgacg ctcttctgct tcctgctctc gggcggcctc gcgggccttg ccggcgccag
     3541 cgacgtcatg ggcgtctcgg gccagctgca gacgccctat gcgccgaact acggcttcgt
     3601 tgccatcatc gtggccttcc tctgccgcct caatcccatc ggcgtgatct tcgccgggct
     3661 cctcctggcg ctgagctaca tcggcggcga gagcgcgcag gtctcgctcg gcatctccga
     3721 caagatcgcc aaggtctttc agggcatcct gctcttcttc attctggcct gcgacacgct
     3781 catcctctac cgcatccgca tcgagagcgg gcgggcggcg caaacgcgga cggcgggcta
     3841 gcgccatgga catgatgatc gccgtagtcc tcactatcat gacagccgcc acgccgctgc
     3901 tgctcgcagc ccttggcgaa ctggtggtgg aacggtccgg cgtgctcaat ctcggtgtcg
     3961 agggcatgat ggtcatgggc gccgtctgcg gttttggcgc ggcgctgatg accggctcgc
     4021 cctggctggg gctgctcgcg tcgattgccg ccggcatgct catgtcgctg ctcttcggct
     4081 ttctcaccct ctcgctggtt tcgaaccagg tggcgaccgg cctggcgctg accctgctgg
     4141 gcctcggcct ctccggccag atcggcgagt ccttcgtcgg ccagccgggc gtgaagctgc
     4201 cgaactacga tgggttcgac cccttgatcc ccttcgccat cctgctggcc tggggcgtgt
     4261 cgtggttcct tttcaagacc aaggcgggtc tcatcgtccg ggcggtgggc aacagccatg
     4321 cctcggcgca tgccatgggc tatcacgtca tccgggtgcg ctatctctgc gtgctcttcg
     4381 gcggcgcctg cgccggactg gccggcggct atctctcgct ggtctatacg ccgcagtgga
     4441 tcgagaacat gacctcgggc cgcggctgga ttgcgctggc gctggtggtc ttcgcctcct
     4501 ggcggccctg gcgcaccgtg gccggtgcct atcttttcgg cgccatctcg atccttgcct
     4561 tcaccgtcca gacttttgcg tggggaatcc cctcgcagtt cttgtcaatg ctgccctatc
     4621 ttgtcacgat catcgccctc gtggtcatct ccggcaaccg gatgctgacg agagcgaata
     4681 cgccggcatg cctgggtcag cccttcgtgc ctgaccgctg aaccaaaaac aaacagggag
     4741 tagtagaatg aagaaactca tgatgtccgc cgccgccttc gccatggcgc tgggcgtggc
     4801 ggcggccagt gccgacgccg aagccaagac caaggcctgc tgggtctatg tcgggtcgat
     4861 cggcgacttc ggctggacct acgagcacaa tcagggccgc ctcgcggtcg agaaggaact
     4921 gggcgacaag gtcgagacgg cctatgtcga gaacgttccg gaagccgact ccgagcgtgc
     4981 catcgagcgc ctggcccgcg acggttgcaa catcatcttc acgacgtcct tcggcttcat
     5041 ggagccgacg ctgaaggttg ccgccaagtt tccggacgtt aagttcgagc acgccaccgg
     5101 ttacaagact gccgaaaacg tgacgaccta caatgccaag ttccacgagg gccgctacgt
     5161 gatcggccag atcgcggcca agatgtcgaa atcgggtacg gcgggctaca tcgtgtcctt
     5221 cccgatcccc gaggtcattg ccggcatcaa cgccttcatg ctgggcgcgc agtctgtcaa
     5281 tccggacttc aaggtgaaga tcgtctgggc caatacctgg tacgatcccg gcaaggaagc
     5341 cgacgccgcc aaggtgctct tcagccaggg cgccgacatc atcgttcagc ataccgactc
     5401 caccgcgccg ctgcaggtgg ccgaggagca gggcaagcag ggcttcggcc aggcgtccga
     5461 catgatcaag ttcgcgccca aggcgcagct caccgccatc gtcgaccact gggcgcccta
     5521 ctacgtgcgc cgcgtgaacg acgtgatcaa cggcacctgg aagagccagt cgtcgtggga
     5581 tggcatggcc gaaggcacgg tgcagattgc gcccttcacc aacatgccgg acgatgtggc
     5641 caagatggcc agcgaaacgg cggaagccat caagaccggc aagctgcatc ccttcaccgg
     5701 tccgatcacc aagcaggacg gcacggttgt tggtgaagcc ggcaaaccgc tgcccgatgg
     5761 cgatatcctg ggcatgaact ggtacgtgaa gggcgtcgac gacaagctgc
//
LOCUS       DBNGVR010000263         3009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_488837, whole genome shotgun sequence.
ACCESSION   DBNGVR010000263 DBNGVR010000000
VERSION     DBNGVR010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_488837"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="AC16A0_05630"
     CDS             complement(<1..173)
                     /locus_tag="AC16A0_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR3104846.1"
                     /translation="MRIDAISIGKNPPKEVNVIVEVPVGGEPIKYEMDKAAGTLVVDR
                     FLYTSMRYPGNYG"
     gene            216..803
                     /locus_tag="AC16A0_05635"
     CDS             216..803
                     /locus_tag="AC16A0_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104847.1"
                     /translation="MKRVAIPFIFGLWPAVAGAAEITSAYTRFDLAKCQVIEKGDEYV
                     FAGVWRCPGLGGVDIFQASSDDRSYAAFGRDGTAHCAFRKTFGPLNTALSPVEWRLRD
                     GKPFAAIERWAVFKDDAVNSYTWLVITALRPADSCPVHYVAGSFPAANQQARDMADRL
                     AEGFDCETGVPTYDSTIGPPPIELLSCAAVKQQGQ"
     gene            complement(809..2641)
                     /gene="typA"
                     /locus_tag="AC16A0_05640"
     CDS             complement(809..2641)
                     /gene="typA"
                     /locus_tag="AC16A0_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR3104848.1"
                     /translation="MNLRNIAIIAHVDHGKTTLIDRLLSQSGSFRDNQKVAERAMDSN
                     DLERERGITILAKVTSLTWKDTRINIVDTPGHADFGGEVERILNMVDGAVLLVDAAEG
                     PMPQTKFVLQKALKIGLRPIVCINKIDRPDERHNEVVNEVFDLFANLDASEEQLDFPI
                     IYGSAKQGWMADSPTGPQDGMAPLFDLVVKHFNPPVVEDGPFRMLVTTIESNPFLGRV
                     LTGRVRSGAIKANQSVKALARDGKTVEQGRISKVLAFRGLERVPVDEAEAGDIIAISG
                     LTTATVADTICDSSVSEPIAAQPIDPPTLTMTFRINDGPLAGKEGDKVQSRVIRDRLL
                     REAEGNVALKVTASETETDAFEVAGRGELQLGILIETMRREGFELTVGRPRVVFRTDE
                     ETGEKLEPVEEVIIDVDEAYTGAVVQKLSERKGELKDMRPSGAGRQRIVFHAPTRGLI
                     GYQSELLSDTRGTAIFNRIFHGYAPHFGEIPGRHTGALISNSDGEAVAYAIFNLQDRG
                     PMFIEHGTKVYPGMIVGEHTRGNDLELNVIKGKKLSNVRASGKDEALILTPPIKMSLE
                     KALAYVGEDELVEVTPKSIRLRKAILDPHERKRVSRAKEAESAA"
     gene            2762..>3009
                     /locus_tag="AC16A0_05645"
     CDS             2762..>3009
                     /locus_tag="AC16A0_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104849.1"
                     /translation="MFESGKHTARTLIALTFTDGRTETVSVRLGLTAKLQDSLNNGDM
                     FLDVVNAADKQYFVAKSSILRVELVEVPKASQMNLQRRA"
BASE COUNT          488 a          952 c          982 g          587 t
ORIGIN      
        1 aagccgtagt tgcccgggta gcgcatcgag gtgtagagga agcggtccac gaccagcgtg
       61 cctgcggcct tgtccatttc gtacttgatc ggctcgccgc caaccggcac ctcgacgatg
      121 acattgactt ccttgggcgg attcttgccg atggaaatgg cgtcgatgcg catgctggct
      181 tcctcttgtt gtcgcttcgt ggcagtctgt ctgccatgaa aagagttgcc attccattca
      241 tcttcggact gtggcccgcg gtcgcgggcg ccgccgagat caccagcgcc tatacccgat
      301 ttgacctcgc caagtgccag gtcatcgaga agggcgacga gtatgtcttc gcaggtgtct
      361 ggcgctgccc cggccttggc ggcgtcgaca tcttccaggc cagttccgac gaccgcagct
      421 acgccgcctt cggccgcgac gggaccgcgc actgcgcctt ccgcaagacc ttcggtcccc
      481 tcaacacagc attgagtccg gtcgaatggc gcctgcgcga cggcaagccc ttcgccgcca
      541 tcgaacgctg ggcagtgttc aaggacgacg cagtgaattc ctatacctgg ctggtgatca
      601 cggcgctccg cccggcggac tcctgccctg tgcactatgt ggcaggctcc tttcccgccg
      661 ccaaccagca ggcccgcgac atggcagacc ggctggccga gggcttcgat tgcgagaccg
      721 gcgtgccgac ctatgattcg accatcggcc cgccgcccat cgaactcctg agctgtgctg
      781 ccgtgaaaca gcagggtcag tagaaggtct acgcggcgct ttcggcttcc ttggcgcggc
      841 tgacccgctt gcgctcgtgc ggatcgagga tcgccttgcg caggcggatc gacttcggtg
      901 tcacctcgac gagttcgtcc tcgcccacat aggcgagcgc cttctcaagc gacatcttga
      961 tcggcggcgt caggatcagc gcctcgtcct tgcccgaggc gcggacgttg gacagtttct
     1021 tgcccttgat cacgttgagc tcgaggtcgt tgccgcgcgt gtgctcgccc acgatcatgc
     1081 ccggatagac cttggtgccg tgctcgatga acatcggtcc gcggtcctgc aggttgaaga
     1141 tggcataggc caccgcctcg ccgtcgctgt tggagatcag cgcgccggtg tggcggccgg
     1201 ggatctcgcc gaaatgcggc gcatagccgt ggaagatgcg gttgaagatc gccgtgccgc
     1261 gcgtgtccga gagcagttcc gactgatagc cgatgagtcc gcgcgtcggc gcgtggaaga
     1321 cgatgcgctg gcggcctgcg cccgagggcc gcatgtcctt gagctcgccc ttgcgttcgg
     1381 aaagcttctg aaccacggcg ccggtatagg cctcgtcgac gtcgatgatg acctcctcga
     1441 ccggttccag cttctcgccg gtctcctcgt cggttcggaa gaccacgcgc gggcggccca
     1501 cggtgagctc gaagccctcg cgccgcatgg tctcgatcag gatgccgagc tgcagttcgc
     1561 cgcggccggc cacttcgaag gcgtcggttt cggtttcgga ggccgtcacc ttcagcgcca
     1621 cattgccttc cgcctcgcgc agcagacggt cgcggatgac ccggctctga accttgtcgc
     1681 cctctttgcc ggcgagcggc ccgtcgttga tgcggaaggt catggtcagc gtgggcggat
     1741 cgatgggctg ggcggcaatg ggctcgctga cgctcgaatc gcagatcgtg tcggccaccg
     1801 tggccgtcgt caggccggag atggcgatga tgtcgccggc ctcggcctcg tccaccggca
     1861 cacgctcgag gccgcggaag gccagcacct tggagatgcg gccctgctcg acggtcttgc
     1921 cgtcgcgcgc cagagccttg accgactggt tcgccttgat cgcgccggag cgcacgcggc
     1981 cggtgagcac gcggccaagg aaggggttcg actcgatggt cgtcaccaac atgcggaagg
     2041 ggccatcctc gaccaccggc ggattgaaat gcttcaccac caggtcgaag agcggcgcca
     2101 taccgtcctg cggcccggtc gggctgtcgg ccatccagcc ctgcttggcc gagccgtaga
     2161 tgatcgggaa gtcgagctgc tcctcgctgg catcgagatt ggcgaagaga tcgaagacct
     2221 cgttcaccac ctcgttgtgg cgttcgtccg gacggtcgat cttgttgatg cagacgatgg
     2281 gacgcagccc gatcttgagg gccttctgca gcacgaactt ggtctgcggc atggggcctt
     2341 cggcggcgtc caccagcaac acggcaccgt ccaccatgtt gaggatgcgc tccacctcgc
     2401 cgccgaagtc ggcgtggccc ggcgtgtcga cgatgttgat gcgggtgtct ttccaggtga
     2461 gggaggtgac cttggccagg atggtgatgc cgcgctcgcg ctcgagatcg ttggagtcca
     2521 tggcacgttc ggcaaccttc tggttgtcgc ggaacgaacc ggactgcgac aggagccggt
     2581 cgatcagggt ggtcttgccg tggtcgacgt gggcaatgat ggcgatgttg cgcagattca
     2641 tggggcgcga agactttctt aactgtgtgg aatgggcggc gcttagcacg gacgatccgc
     2701 aaaggcaatt taacccttgt taccggccga gttgctagag tccccggttc ggagggtcac
     2761 aatgttcgaa agcggcaagc acacggcgcg gacgctgatt gcgctgacat tcacagacgg
     2821 caggacggag acagtgtcgg tcaggctcgg cctcaccgcc aagctccagg attcccttaa
     2881 caacggtgac atgttcctcg atgtcgtcaa tgccgccgac aagcagtact tcgtggccaa
     2941 gtccagcatt ctgcgtgtcg agttggtgga agttcccaag gccagccaga tgaacctgca
     3001 gcgccgcgc
//
LOCUS       DBNGVR010000264         3652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3042716, whole genome shotgun sequence.
ACCESSION   DBNGVR010000264 DBNGVR010000000
VERSION     DBNGVR010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3042716"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="AC16A0_05650"
     CDS             <1..1384
                     /locus_tag="AC16A0_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3104850.1"
                     /translation="DAVEQLLDGKITSFDQEFRMRHQDGHFVWLRARVALAPGETENQ
                     PHLIGIVFDITQQKLADKLNKEAELRLMDAVENISEAFVLWDADNRLVLCNSKYQQFH
                     SLPASVCAPGTPYEDVVKTAKEPAVRKRIGLKGESGEGRSFEVQLADRRWLQINERRT
                     KDGGFVSVGTDITALKRQEERLLLSERELMMTVRDLQKERLLADQQAQRLADLADKYA
                     REKTRAEAANRSKSEFLANMSHELRTPLNAIIGFSEIMVAQIFGVLGSDKYREYANDI
                     HRSGQFLLDVINDILDMSKIEAGRMQLEVERFDLNAMFEEVMRLVGPRAFEGHVTIER
                     HIADALQAEADKRAIKQVVINLMSNAVKFTPEGGKVEVSAGLAGDNIVIRISDTGIGI
                     PARDIEKLGRPFEQAENQFTKSKGGSGLGLAISKSLVDLHGGRLDIESKVGAGTIVTV
                     TLPQPAKVAA"
     gene            1627..2133
                     /locus_tag="AC16A0_05655"
     CDS             1627..2133
                     /locus_tag="AC16A0_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HIR3104851.1"
                     /translation="MTQDAVTAAPPRWRIVLGVASFITAFAVHLITLAAVGIGASPAL
                     VSAIGAVNFVVNKVLVLASAAFLGRAGFDQLKTFVFGAVRRLAFPDEVSPFRYRAGLL
                     LLLIPIFLAWISPYLADLAPSFGRNTIRDGVIGDALLLIGLLLLGGDFWDKLRALFVR
                     NAKVAFPD"
     gene            2443..3381
                     /locus_tag="AC16A0_05660"
     CDS             2443..3381
                     /locus_tag="AC16A0_05660"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR3104852.1"
                     /translation="MLELIFSALAFAGLWAAMWWLRDMSIVLTLVLAIPTAGFMVRLF
                     MIQHDCGHGSFFKSRLANDWIGRIISVFTLTPYDFWRQSHAMHHAGVGNLDRRGIGDI
                     DMLTVREFQALRGHGRLRYRLYRNPLVMFGLGPAYLFLLRNRLPFGAMRSGAMPWIST
                     MLTNLATVAASGVAVYAVGAGTFLAIQIPVVLIGASIGVWLFFVQHQFERTYWEHQPD
                     WSHPEAALHGSSFYDLPRLLMWMTGNIGIHHLHHLSCRIPFYNLPEALADHPQFRQVG
                     RLTLWQSLRCARLSLWDEDKRRLVSFAEVRAAQAAV"
     gene            complement(3445..>3652)
                     /locus_tag="AC16A0_05665"
     CDS             complement(3445..>3652)
                     /locus_tag="AC16A0_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104853.1"
                     /translation="ASALEATSYDEARVRAVIAQINATSSGLIDRGGDAAITFIAALT
                     PEERGKLAKRILERVNRGSGPPGD"
BASE COUNT          666 a         1176 c         1092 g          718 t
ORIGIN      
        1 ggatgcggtg gaacagctgc tcgacggcaa gatcacctcc ttcgaccagg aattccgcat
       61 gcggcaccag gacggccatt tcgtgtggct ccgcgcgcgc gtggcgctcg cccccggtga
      121 aacggaaaac cagccgcacc tgatcggcat cgtcttcgac atcacccagc agaagctcgc
      181 cgacaagctc aacaaggagg ccgagctccg cctgatggac gcggtcgaga acatctcgga
      241 ggccttcgtg ctttgggatg ccgacaaccg cctcgtgctg tgcaattcga aataccagca
      301 gttccactcg ctcccggcat cggtctgcgc gcccggcacg ccctatgagg acgtggtgaa
      361 gaccgccaag gaacccgcgg tgagaaagcg catcggcctc aagggcgaga gcggcgaggg
      421 ccgctccttc gaggtgcaac tggccgaccg tcgctggctg cagatcaacg agcgccgcac
      481 caaggacggc ggcttcgtct cggtcggtac cgacatcacc gcgctgaagc gccaggagga
      541 gcggctgctg ctgtccgaac gcgaactgat gatgacggtg cgcgacctgc agaaggaacg
      601 cctgctcgcc gaccagcagg cacagcggct ggccgacctt gccgacaagt atgcccgcga
      661 gaagacgcgt gccgaagctg ccaaccggtc gaagtccgag ttcctcgcca acatgagcca
      721 cgagctgcgc accccgctca atgccatcat cggattcagc gagatcatgg tggcgcaaat
      781 cttcggcgtg ctggggtccg acaagtatcg cgagtatgcc aatgacatcc accgcagcgg
      841 acagttcctg ctcgacgtca tcaacgacat tctcgacatg tcgaagatcg aggccggtcg
      901 catgcagctc gaagtcgagc gcttcgatct caacgccatg ttcgaagagg tgatgcggct
      961 ggtggggcct cgtgccttcg aaggccacgt caccatcgaa cgccatatcg ccgacgccct
     1021 ccaggccgag gccgacaagc gcgccatcaa gcaggtggtc atcaacctca tgtcgaatgc
     1081 ggtgaagttc acgccggaag gcggaaaggt cgaggtttcg gcgggactgg cgggcgacaa
     1141 catcgtcata cgcatcagtg acaccggcat cggcatcccg gcccgcgaca tcgagaagct
     1201 tggccggccc ttcgagcagg ccgagaacca gttcaccaag agcaagggcg gctcgggtct
     1261 cggccttgcg atctcgaagt ccctcgtcga tctgcacggc ggccggctcg acatcgaaag
     1321 caaggtcgga gccggcacca tcgtgaccgt gaccctgccc cagcccgcga aggtcgccgc
     1381 ttagggcgga tctcgatttc tgctcgggtg atcaatctgt gcccagaggg agcctgaatc
     1441 gcctctccat tcagctcgca tgccggcaca gctggaaaag cgtgccatgt catggcaagc
     1501 tgcgtccgca tccgccttgc gatcggcaac cttgtcccgt agcaacgcca tcgcagcgga
     1561 tttgcgccat caacgcgtca ctcgtttcgc caacatgccg gagctggcca aaggccaggg
     1621 atcaagatga cacaggacgc cgtcactgct gctccacccc ggtggcgaat tgtgctcggt
     1681 gttgcctcct tcatcaccgc cttcgccgtt cacctcatca cgcttgcagc ggtcggtatt
     1741 ggcgccagcc cggctctcgt cagcgcgatc ggcgccgtga atttcgtggt caacaaggtg
     1801 cttgttcttg ccagcgcggc attccttggc cgcgccgggt tcgatcaact caagaccttc
     1861 gtcttcgggg ctgttcgacg cctcgctttt ccagatgaag tgagtccctt tcgctatcga
     1921 gcaggactct tgcttctttt gatcccgatc tttcttgcct ggatttctcc ctatctcgcc
     1981 gaccttgccc cgtccttcgg ccgaaatacc atacgcgacg gcgtgatcgg cgatgcattg
     2041 ctcctcattg gcttgctgct gctgggtggc gacttctggg acaagctcag ggcgctcttc
     2101 gtgcgcaatg cgaaggtcgc attccccgat tagcgccgca actgtcggac acgggccaag
     2161 gccgagggtc gctgtccgcg ggaattcgaa agcaatacag ggccttgcaa cgggcggcga
     2221 acccagtcat cttgccgtta cgccactctc gcgccgtctc tgcaaactca tgcggtccag
     2281 ccctcgaaaa accgcagtgt gatggtccaa gggcgcccgc tgagcaacag ctgcctctga
     2341 aagtggatat caacgcgtgg gaaactccag atcgtgacag tcaagacggc gtgcttgtcg
     2401 gcaagtctgg cagtccacag gaaccccaga acgcttcgct cgatgctgga gctcatcttt
     2461 tcggccttgg catttgccgg cctctgggcg gccatgtggt ggctgagaga catgagcatt
     2521 gtgctgacgc tcgttctggc catcccgaca gccggcttca tggtgcggct cttcatgatc
     2581 cagcatgact gtggtcatgg ctccttcttc aagtcccggc tcgccaatga ctggatcggc
     2641 cgcatcatca gcgtctttac cttgacgccc tatgatttct ggcgacagtc ccatgccatg
     2701 caccatgccg gagtcggcaa tctcgaccgc cgcggcattg gcgacattga catgctgacc
     2761 gtccgcgagt ttcaggcatt gcgcggtcat ggacggctgc gctaccggct ctatcgcaat
     2821 ccgctcgtga tgttcgggtt gggaccggcc tatctgttcc tgctacgaaa tcgccttcca
     2881 tttggcgcga tgcggtccgg ggcgatgcca tggatcagca ccatgctcac gaacctcgca
     2941 actgtggcag cctcgggagt ggccgtttac gccgtgggag ctggaacctt ccttgcaatc
     3001 cagattcccg tcgtgctgat cggcgcatcc atcggagtct ggctgttctt cgtgcagcat
     3061 cagttcgagc gcacctattg ggaacaccaa ccggattggt cacatccaga ggcagcgctg
     3121 catggcagct ccttctatga cttgccgcgc cttctgatgt ggatgaccgg aaacatcgga
     3181 atccatcacc tgcatcatct gtcttgccgg atacccttct acaatcttcc cgaggcgctt
     3241 gcggaccatc cacaattccg ccaggtcggc cgcctgacgc tctggcagag cctccgatgc
     3301 gcgcggctgt cgctgtggga tgaggacaag agacggctcg tgtccttcgc cgaggttcgg
     3361 gcagcgcagg ccgcggtctg atgcgttgcc tcgctggggg aaggccgatg gatcgccggc
     3421 ctggatcgcg acgccctcaa tcggtcagtc gcccggcggg cccgatcccc ggttgacccg
     3481 ctcgaggatt cgcttggcca gtttgccgcg ctcctctggc gtgagggcgg caatgaaggt
     3541 gatcgccgca tcgccgccgc gatcgatcaa gccggagctg gtggcattga tctgggcaat
     3601 gaccgcacgc accctggcct cgtcatagga tgttgcttcc agcgcgctgg cg
//
LOCUS       DBNGVR010000265         2837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1866070, whole genome shotgun sequence.
ACCESSION   DBNGVR010000265 DBNGVR010000000
VERSION     DBNGVR010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1866070"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="AC16A0_05670"
     CDS             <1..1036
                     /locus_tag="AC16A0_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3104854.1"
                     /translation="QNLFRVANEKTTFAAAFAMADQVLYSGVASITELMTKDGLINLD
                     FADVRAIMSEMGRAMMGTGEATGEKRAIEAAEAAISNPLLDDVSMRGARGLLISITGG
                     PDLTLYEVDEAATRIREEVDPEANIILGATFDESLEGTMRVSVVATGLASVADRQSEP
                     ASEDKAEPEETGRAVFGFTGKQPTPVKKHAQPAPMAARATVQQAQPPQHSHDHYDAEE
                     EADMVQQAVAEETPRQQPRPVRIPPINEFPPIAQQQMAAQQRSIESIADHAGRKKKGL
                     FERLASVGLGRKDEPAPVAQREPTMASQPRPQQVAPIRPQAPAEQHRPEPALDDDQLE
                     IPAFLRRQAN"
     gene            1141..2109
                     /locus_tag="AC16A0_05675"
     CDS             1141..2109
                     /locus_tag="AC16A0_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3104855.1"
                     /translation="MYDTGLKSWESDKVHSFDIRRASRGAARNGAAAVVLLILALPLG
                     GCATSIMDGIWGDDSSSTAAAGVGTGLSPDMATAAEGEAVAKLYNEALESLKKNNYKT
                     AVKEFGEVERQYPYSSYATKAILMQAYVNYKAKKYDDAIVAANRFITLHPGHKDAAYA
                     HYLVALSDFERINDTKRDQTSTRKALEALEEIERRFPDTPYAEDASQKVLVARDHLAG
                     KEMQIGRYYYKQGSYLAGINRFKRVVTEYQNTSQTPEALYRLSEGYMALGVVSEAQTA
                     AAVLGHNFPNSEWYKDAYQLVSSDGLAPVENRESWISKTFRAINPL"
     gene            2157..>2837
                     /locus_tag="AC16A0_05680"
     CDS             2157..>2837
                     /locus_tag="AC16A0_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3104856.1"
                     /translation="MLTALSIRDIVLIEKLDLSLDRGLTVLTGETGAGKSILLDSLGL
                     ALGARGDSTLVRAGAERGVVTAAFALPASHMAFSVLKEQGIEADGELVIRRIQNADGP
                     SRAAINDQPVSLNLLRQIAGTLAEIHGQHADRALVDVGAHRRLLDAFGALEGASAEVA
                     VLWDKTSVAQAELEAHRSLMARAEAERDYLEHAAEELRVLNPVDGEEAKLAEARQMMM
                     SAEQYAEAI"
BASE COUNT          572 a          920 c          882 g          463 t
ORIGIN      
        1 ccagaacctc ttccgagtcg ccaatgaaaa gaccaccttc gccgcggcct tcgccatggc
       61 cgaccaggtg ctctattcgg gcgttgcctc gatcaccgaa ctgatgacca aggatggcct
      121 gatcaacctc gacttcgccg atgtgcgcgc catcatgagc gagatgggcc gcgccatgat
      181 gggcacgggc gaggcgacag gcgagaagcg cgccatcgaa gcggcggaag ccgccatctc
      241 caatccgctg ctcgacgatg tctcgatgcg cggagcccgc ggcctgctca tctcgatcac
      301 cggcggtccc gaccttaccc tctacgaggt cgacgaggcc gcaacccgta tccgtgagga
      361 agtggatccc gaggccaaca tcatccttgg cgccactttc gatgaatcgc tggaaggcac
      421 catgcgggtc tcggtggtcg ccaccgggtt ggcatccgtg gctgaccgcc agtccgaacc
      481 cgcctccgaa gacaaagcgg agccggaaga gacaggccgt gccgtcttcg gattcaccgg
      541 caagcagccc acgcccgtca agaagcatgc gcagcccgcg cccatggcgg cccgcgcgac
      601 cgtccagcag gcacagcctc cgcagcattc ccacgaccac tatgatgccg aggaagaggc
      661 tgacatggtc cagcaggcgg tggcggaaga gacaccgcgc caacagcccc ggcccgtgcg
      721 cattccgccg atcaacgaat tcccgccgat tgcgcagcag cagatggcgg cgcagcagcg
      781 cagcatcgag agtattgccg atcatgccgg ccgcaagaag aagggcctct tcgagcgtct
      841 tgcaagtgtc ggccttggcc gcaaggacga gccggcgccg gtcgcgcaac gcgagccgac
      901 catggcttcc cagccgcgac cgcaacaggt cgcgcccatc cgcccgcagg cgccggccga
      961 gcagcaccgc ccggaacccg cgctcgacga tgatcagctg gagattcccg ccttcctgcg
     1021 ccgccaggct aactagctga aactcatctg cgttccgtga agcccggcgg tcacacttcc
     1081 gccgggtttc gtgcctctaa cggtcgtcgc cggggggtgc tggcatggcc gagacctgtg
     1141 atgtatgaca ccggcctcaa gagttgggaa tcggacaaag tgcattcatt cgatatcagg
     1201 agagcgtctc gtggggccgc gcgcaatggt gcagccgctg tcgtcctcct gatcctggcc
     1261 ttgccccttg gtggctgtgc cacgagcatc atggatggaa tctggggcga tgattcgtct
     1321 tcaacagcgg cagccggcgt cggcaccggc ctctcgccgg acatggcaac cgccgccgag
     1381 ggcgaggccg tggcgaagct ttacaatgaa gcgctcgaga gcctgaagaa gaacaattac
     1441 aagacggcag tcaaggaatt cggcgaggtc gaacggcagt atccctattc gagctacgcc
     1501 accaaagcca ttctcatgca ggcctatgtg aactacaagg ccaagaaata cgatgatgcg
     1561 atcgtggcag caaaccgctt catcacgctg catcctgggc acaaggatgc ggcctacgcg
     1621 cattatctcg ttgcgctgtc cgattttgag cggatcaacg ataccaagcg agatcagact
     1681 tccacgcgca aggcgctgga ggcgctcgag gagatcgagc gccggttccc cgacacgccc
     1741 tatgccgaag acgcaagcca gaaggtactg gtggcgcgcg accatctggc aggcaaggaa
     1801 atgcagatcg gccgctacta ctacaagcag ggctcctatc tggcgggcat caaccgcttc
     1861 aagcgggtgg tgaccgagta tcagaacacg tcccagacgc ccgaagcgct ctatcgcctc
     1921 tcggaaggct atatggcact gggcgtcgta tcggaggccc agacggccgc agccgtgctg
     1981 gggcacaatt tccccaattc ggaatggtac aaggatgcct accagctcgt ctccagcgac
     2041 gggctggcgc cggtcgaaaa cagggaaagc tggatcagca agaccttccg ggcgatcaat
     2101 cccctgtaat ccacaccccc tcgcgcgctc cgccggattt ccggtataag gcgcctatgc
     2161 tcaccgcact ttccatccgc gacatcgtgc tcatcgagaa gcttgatctc tctctcgacc
     2221 ggggcctcac cgtcctcacc ggcgagacgg gcgcggggaa gtccatcctg ctcgactctc
     2281 tgggactggc cctgggcgcc agaggggatt ccaccctcgt gagggccggt gccgagcgcg
     2341 gtgtcgtaac tgcagccttc gcgttaccgg cctcgcacat ggctttttcc gtgctcaagg
     2401 aacagggcat cgaagctgac ggcgaactgg tgatccggcg cattcagaat gccgacggac
     2461 cgagccgtgc ggcgatcaat gatcaaccgg tcagcttgaa tctgctgcgg cagattgccg
     2521 gtacgctggc cgagatccac ggccagcacg ccgatcgtgc gctggtcgac gtcggcgcac
     2581 accggcggct gctcgatgcc ttcggcgcgc tcgagggcgc atcggcagag gttgcggtcc
     2641 tatgggacaa gacaagtgtg gcgcaggctg aacttgaggc acatcgcagc ctgatggcgc
     2701 gggccgaggc cgagcgagac tatctggagc atgcagccga agagcttcgc gtgctgaacc
     2761 ctgtcgacgg cgaggaagcc aagctggccg aagcgcgcca gatgatgatg tcggccgagc
     2821 aatatgccga agcgatc
//
LOCUS       DBNGVR010000266         3478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_686280, whole genome shotgun sequence.
ACCESSION   DBNGVR010000266 DBNGVR010000000
VERSION     DBNGVR010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_686280"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16A0_05685"
     CDS             complement(<1..236)
                     /locus_tag="AC16A0_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HIR3104857.1"
                     /translation="MNELDLKALGVTGINQRLYKVPKDSNERHWVITNPMGQHAIACG
                     LDGPLQVDIKGHVGFYCGGMNKEAEIIIHGHAGVG"
     gene            complement(233..1135)
                     /locus_tag="AC16A0_05690"
     CDS             complement(233..1135)
                     /locus_tag="AC16A0_05690"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR3104858.1"
                     /translation="MCGIVGLYLKNPKLQSKLGAMFKPMIIEMTNRGPDSAGVAIYRN
                     PVKKAEVKFSLAHDDPAYNWKGVDTGLEKALKCDAKIKVIGNHCILVTTAKEDKVVHW
                     LKKTHPQVRVVGSGHTIEIFKETGLPENVYQKFDLSKASGTHIIGHTRMATESAVTTA
                     GSHPFATGADLCLVHNGSLSNHNRLRENLKREGMEFQTENDTEVAAAYMTWKIRNGAT
                     LKQALEDGIKDLDGFYTFLVGTADGFAVVRDPIACKHAVMAETEDWVAMATEYRAIAH
                     LPGADDAKIWEPEPTTIYTWGGHA"
     gene            complement(1251..1913)
                     /locus_tag="AC16A0_05695"
     CDS             complement(1251..1913)
                     /locus_tag="AC16A0_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3104859.1"
                     /translation="MDKRAPAKGKTRGKDKGLPFQDPHASPSRRANLLEEAIGREVKG
                     FREKLGLTIAELAKAADMSAGMLSKIENGATSPSLASLQALGKALQVPVTALFRGFEE
                     IRDATFVKAGQGLTIERRGTRAGHQYQLLGHSPHGPVMVEPYLITLTQQSDVFPTFQH
                     AGFEFLYMLEGNVVYRHGDKTYDMHPGDALFFDADAPHGPEDLRQLPARYLAVISYRR
                     EE"
     gene            2299..3213
                     /gene="cysD"
                     /locus_tag="AC16A0_05700"
     CDS             2299..3213
                     /gene="cysD"
                     /locus_tag="AC16A0_05700"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR3104860.1"
                     /translation="MTSHPDRLTHLERLEAESIHIMREVAAECEKPVMLYSIGKDSAV
                     MLHLAIKAFYPAKPPFPLMHIDTGWKFRDMYAMRAATAEKYGWELLVHKNPEGVAKNV
                     NPFDHGSQLHTHIMKTEGLKQALDKHGFDAAFGGARRDEEKSRAKERIFSFRDSRHRW
                     DPKNQRPELWNLYNARKNKGESIRVFPLSNWTELDIWQYIHKEGIDIVPLYFAAERPI
                     VWRDGQMIMVDDERMRLNPGEVPEMRNVRFRTLGCYPLTGAIDSNAATLLDIVEEMLV
                     ATSSERQGRVIDKDQSASMEKKKQEGYF"
     gene            3213..>3478
                     /locus_tag="AC16A0_05705"
     CDS             3213..>3478
                     /locus_tag="AC16A0_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3104861.1"
                     /translation="MTRFIEFTGGDVEAYLQAQEQKDLLRFITCGSVDDGKSTLIGRL
                     LYEAKMLFEDQLTALEADSKKMGTQGGNLDFALLVDGLAAEREQG"
BASE COUNT          652 a         1097 c         1057 g          672 t
ORIGIN      
        1 ccgacaccgg cgtggccatg gatgatgatc tcggcttcct tgttcatgcc gccgcaatag
       61 aatccgacat gccccttgat atccacctgc agcggcccgt cgaggccaca ggcgatggca
      121 tgctgtccca tcggattggt gatgacccag tgccgctcgt tcgagtcctt gggaacctta
      181 tagagacgct ggttgatgcc ggtgacgccc agcgccttga gatcgagctc gttcatgcgt
      241 gtccgcccca ggtgtagatg gttgtcggtt caggttccca gatcttggcg tcatcggcac
      301 ccggcagatg ggcaatggcg cgatactccg tcgccatggc cacccagtcc tcggtctcgg
      361 ccatcaccgc atgcttgcag gcgatgggat cgcgcaccac cgcgaagccg tcggcggtac
      421 cgacgaggaa ggtgtagaaa ccgtcgagat ccttgatgcc gtcttccaag gcctgcttca
      481 gcgtggcgcc gttgcggatc ttccaggtca tgtaggcggc cgccacttcg gtgtcgttct
      541 cggtctggaa ctccatgccc tcgcgcttca ggttctcgcg caggcggttg tggttcgaca
      601 gcgaaccgtt gtgcacgagg cagagatcgg caccggtggc gaagggatgc gaccccgccg
      661 tggtcaccgc gctctcggtg gccatgcgcg tatggccgat gatgtgcgtg ccggatgctt
      721 tggagagatc gaacttctgg tagacgttct ccggcaggcc ggtctccttg aagatctcga
      781 tggtgtggcc cgagcccacc acgcgcacct gcggatgggt cttcttcagc caatggacca
      841 ccttgtcttc cttggccgtg gtcaccagga tgcagtgatt gccgatgacc ttgatcttgg
      901 cgtcacactt gagcgccttc tccagtcccg tatcgacgcc cttccagttg taggcgggat
      961 catcatgcgc cagcgagaac ttgacctcgg ccttcttcac cggattgcgg tagatggcga
     1021 cgccggcgga atcgggtccg cgattggtca tctcgatgat catgggcttg aacatcgcgc
     1081 ccagcttgga ctgcagcttg ggatttttca ggtagagtcc gacgatgccg cacatgtgtg
     1141 gagcgccctc cgtgaatgat gctggagagg ctagcggcaa gctcgggcga aatcaactgt
     1201 tgggaaacaa taattcactc aggtaaacgc agacggcccc tgcaggcggc tcattcctcg
     1261 cggcggtagg agatcacggc caggtagcgg gccggcagct ggcgcaggtc ttccggccca
     1321 tgcggtgcat cggcatcgaa gaacagggca tcgccgggat gcatgtcata ggtcttgtcg
     1381 ccgtgccggt acaccacgtt gccctccagc atatagagaa actcgaagcc cgcatgctgg
     1441 aaggtgggga agacgtcgga ctgctgggtg agcgtgatga gatagggctc gaccatcacc
     1501 ggcccgtgcg ggctgtggcc caggagctga tactggtgac cggcccgcgt gccgcgccgc
     1561 tcgatggtca ggccctggcc cgccttcaca aaggtggcat cgcggatttc ctcgaagccg
     1621 cggaacagcg cggtgaccgg cacctgcagc gccttgccca gcgcttgcag cgaggcaagc
     1681 gaaggcgagg tggcgccgtt ctcgatcttg gatagcatgc cggccgacat gtccgccgcc
     1741 ttggccagtt cggcaatggt gaggcccaac ttctcgcgga agcctttcac ctcgcggccg
     1801 atggcttctt cgagcagatt ggcgcggcgc gagggcgagg catgagggtc ctggaacggc
     1861 aggcccttgt ccttgccgcg cgtcttgccc tttgctggag cccgcttgtc caatctgttt
     1921 cctcccagga aaattcttag cctcgccagg cttgtggctc aaggccaaga ttgcgctcga
     1981 taggttactc ccttcacgat ctatcaaaat aggttttttc cgctcgactc tttgttgatt
     2041 gatcggtaga aacctatcat ggacatgatc gcaaaggcgt tcctcgccga catcgacaaa
     2101 ttcctgcaac aggcaggcgt cgatccgacc gcccttggca agcaggccat gggcgatccc
     2161 tctttcgtct tcgatctcaa gaagggccgc tcgccctcga cccgcaccat ggagcgcgtg
     2221 cgcgcctgga tgctgcagca gcgcgctgcc gcagcgccgc cccggcctca ccccgcagcc
     2281 acaaaccccg gacctgtcat gaccagccat cccgaccgac tgacccacct ggaacggctg
     2341 gaagccgagt cgatccacat catgcgcgag gtggccgccg agtgcgagaa gccggtgatg
     2401 ctctattcca tcggcaagga ttccgccgtg atgctgcacc tcgccatcaa ggccttctat
     2461 cctgccaagc cgcccttccc gctgatgcac atcgataccg gctggaagtt ccgcgacatg
     2521 tatgccatgc gcgcagccac cgcggagaag tacggctggg aactgctggt ccacaagaat
     2581 cccgaaggcg tggccaagaa cgtcaacccc ttcgaccacg gctcgcagct tcacacccac
     2641 atcatgaaga ccgaaggcct gaagcaggcg ctcgacaagc atggtttcga cgcggccttc
     2701 ggcggcgccc gccgcgacga ggaaaaatcc cgcgccaagg agcgcatctt ctccttccgc
     2761 gattcgcgcc atcgctggga cccgaagaac cagcgccccg aattgtggaa cctctacaac
     2821 gcccgcaaga acaagggcga atcgatccgc gtcttcccgc tgtcgaactg gacggaactc
     2881 gacatctggc aatacatcca caaggaaggc atcgacatcg tgccgctcta cttcgcggcc
     2941 gaacgtccca tcgtctggcg cgacgggcag atgatcatgg tcgacgacga gcgcatgcgc
     3001 ctcaatccgg gcgaagtgcc cgagatgcgc aacgttcgct tccgcacgct gggctgctat
     3061 ccgctgaccg gcgccatcga ctccaatgcg gccacgctcc tcgacatcgt cgaggaaatg
     3121 ctggtggcca cctcctccga gcgccagggc cgggtcatcg acaaggacca gtcggcctcg
     3181 atggagaaga agaagcagga aggatatttc tgatgacgcg cttcatcgaa ttcaccggcg
     3241 gcgatgtcga ggcctatctg caggcacagg aacagaagga cctgttgcgc ttcatcacct
     3301 gcggctcggt cgatgacggc aagtccaccc tcatcggaag gcttctctat gaagccaaga
     3361 tgctgttcga ggaccagctc acggcgctcg aggccgattc gaagaagatg ggcacccagg
     3421 gcggcaatct ggattttgct ttgctggtcg atggccttgc cgccgagcgc gagcaggg
//
LOCUS       DBNGVR010000267         3304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3240779, whole genome shotgun sequence.
ACCESSION   DBNGVR010000267 DBNGVR010000000
VERSION     DBNGVR010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3240779"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="AC16A0_05710"
     CDS             <1..1111
                     /locus_tag="AC16A0_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3104862.1"
                     /translation="LLDMKAGLAAGLAAMEAWQGEGNLLFLAVADEEDRSAGARAAVG
                     QLAAFAKAQDLDIALVINLDAISDQGDGSSGRVAALGSIGKLLLTAFVIGREAHACYP
                     QDGANAAYLAAELLTEFELAPELAEASGSEIAAPPTALAAKDLKTGYNVTTPGQAWCY
                     WNTLQHRRGAEEVLDISLMLARRAMARAARKLGRDIPVMTCADLMARVPHAGSARFGA
                     EIAALTALDLPERSKRMTEALWALSGLSGPSVVIGFGSIPYPAVALSDAALIDIITEA
                     VTPHGLGTVQYFPGISDMSFFGESAGDLAAAAANSPIWGTSFAMPAPAGYPCINIGPW
                     GRDYHHWLERLHAPYAFETLPRVLLAVIEAVMKHR"
     gene            1143..1487
                     /locus_tag="AC16A0_05715"
                     /pseudo
     CDS             1143..1487
                     /locus_tag="AC16A0_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
     gene            1657..2541
                     /locus_tag="AC16A0_05720"
     CDS             1657..2541
                     /locus_tag="AC16A0_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3104863.1"
                     /translation="MALLLFIPALVLVNMVWVDGVTRAAAPRDGGRLVATPRIEANVR
                     VEGEGPPLVLIHGFAASLGWWDQIAPDLAKDHTVVRLDLIGHGGSAAPSAGYAIEDQA
                     QVVASVLDSLNLDPVTVIGHSMGGEVATALAEANPVRIARLILIDSPPEADTKFTGLT
                     ELYLTRGIGELLSHFQSDKAIRDGLAQGFAPGFPVPQRFVDDLRQLTYTAFRGAHDAS
                     VAYRSAAPTYERLAALDPVPPLLAIAGAADAIVPVATAKLFEKVPGAKVEILDGVGHT
                     PMVEAPEKTLALIRGFLP"
     gene            complement(2533..>3304)
                     /locus_tag="AC16A0_05725"
     CDS             complement(2533..>3304)
                     /locus_tag="AC16A0_05725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3104864.1"
                     /translation="KIGFGIHPLNYVRGLARAAAAAGAKIHASSRLTRWEETAAGHVL
                     TTPGGRLTARRVLVATNGYTPEDVSPRHAGRIMPALSSVLVTRPLSEAERRAQGWTSD
                     LMAYDSRNLLHYFRLLPNGRFLFGGRGGTDASAGGGEAYRAVLTRAFHGLFPAWRGVE
                     ITHYWRGFVCLAFDRVPYVGALDAAKTVWTALAYHGNGVAMGSWSGRAVARLMTGKAP
                     PSEIPAVLTRRLARFPLPAFRPLYLKGAYVWFGWKDSR"
BASE COUNT          555 a         1161 c         1107 g          481 t
ORIGIN      
        1 cttgctcgac atgaaggcgg gcctcgccgc aggactggcc gcaatggaag cctggcaggg
       61 cgagggcaac ctgctcttcc ttgcggttgc cgacgaggaa gaccgctcgg ccggcgcccg
      121 cgccgccgta gggcagctcg cggctttcgc gaaggcacag gacctcgaca tcgcgctggt
      181 gatcaatctc gatgccatct ccgaccaggg cgacggcagc agcggccggg tcgcggcgct
      241 gggctccatt ggcaagctgc tgctcaccgc cttcgtgatc gggcgcgagg cccatgcctg
      301 ttacccgcag gacggcgcca atgcggccta tctcgcggcc gagctgctga cggaattcga
      361 gctcgccccc gaactggccg aggccagcgg cagtgagatc gcggcaccgc ccaccgcgct
      421 ggccgccaag gacctcaaga ccggttacaa cgttaccacg ccgggccagg cctggtgcta
      481 ctggaacacg ctgcagcacc ggcgcggcgc cgaagaggtg ctcgacattt cgctgatgct
      541 ggcgcgccgc gccatggcgc gggccgcgcg caagcttggc cgcgacattc cggtcatgac
      601 ctgcgccgac ctcatggccc gcgtgccaca cgccgggtcg gcgcgttttg gcgcggagat
      661 cgcggcactg acggcactcg acctgccgga gcgcagcaag cgcatgacgg aggcgctatg
      721 ggcgctgagc ggcctctccg gtccctccgt ggtcatcggc ttcggctcca tcccctatcc
      781 ggcggtggcg ctctccgatg cggcgctgat cgacatcatc acggaagcgg tgactccgca
      841 tggcctcggc acggtgcagt actttcccgg catctccgac atgagcttct tcggcgaatc
      901 ggcgggcgat ctcgcagcgg ccgccgccaa ctcgccgatc tggggcacca gcttcgccat
      961 gccggcaccg gccggatacc cctgcatcaa catcgggccc tgggggcgcg actatcatca
     1021 ctggctcgag cggctgcatg cgccctatgc cttcgaaact ttgccgcgcg ttttgctggc
     1081 ggtgattgaa gcggtgatga aacatcgcta gagagcgagt gccaaacaag cggaggcaca
     1141 caatggcact caagagactc gaagccggca agcgcatgag ctcggccgtc atccacaacg
     1201 gcatggccta tctcgcgggc ttcgtcgcgg aagcggcggc cggcaagtcg gtcaaggaac
     1261 agaccgccga catcctgcag cagatcgatg cgacgctgaa ggcggcgggc accagcaaga
     1321 ccaacatcgt caaggccaac atctggctca ccgacatcaa gacctgggcc gagatgaacg
     1381 aggcctggga cgcctgggtc gtccccggcc agactccggc ccgcgccacg gtcgaatcca
     1441 ggctcgcggc ccccggcctc gacgtggaga tcatggtgga ggcggccatc gtgaagaagg
     1501 cggctccggc caggaaggcc gccccggcca agaaagcagc aaagaaagcg gcaaagaagc
     1561 ccgccaagaa gccagcgaag aagaagggcc gccgctgatc cacgtttgcc catgcgcaga
     1621 cgccgcagga actgggggac cacgagaggc gctctcgtgg cccttcttct cttcatcccc
     1681 gcgctcgtcc tggtcaacat ggtctgggtc gacggggtca cccgcgccgc cgccccgcgc
     1741 gacggcggac gcctcgttgc aacaccgcgc atcgaggcca atgtccgcgt cgagggcgaa
     1801 ggcccgcccc tcgtcctgat tcacggcttt gccgcctcgc tcggctggtg ggaccagatc
     1861 gcgcccgatc tcgcgaagga tcacaccgtt gtccgtctcg atctcatcgg ccatggcggc
     1921 agcgcggcac cgtccgcggg ctacgcgatc gaggaccagg cgcaggtggt tgcgtccgtg
     1981 ctcgacagcc tgaacctcga ccccgtcact gtcatcggcc attccatggg cggcgaggtg
     2041 gcaaccgcgc ttgccgaggc caatccggtc cgcatcgcgc gcctgatcct gatcgacagc
     2101 ccgcccgagg ccgatacgaa gttcacgggt ctcaccgagc tctatctgac acggggcatc
     2161 ggcgagctgc tgtcgcactt ccagtcggac aaggcgatcc gcgatggcct cgcccagggc
     2221 ttcgcgccgg gctttccggt gccgcaacgc ttcgtcgacg acctgcgcca gctcacctac
     2281 accgccttcc gcggcgcgca tgatgccagc gtcgcctatc gcagcgcggc gcccacctac
     2341 gaacggctcg ccgcgctcga tccggtgccg ccgcttctgg ccatcgctgg cgccgcggat
     2401 gccatcgtgc cggtggcgac ggcgaagctc ttcgagaaag tgccgggtgc gaaggtcgaa
     2461 atcctcgacg gcgtcggcca cacgcccatg gtggaagcgc ccgagaagac gctggcgctg
     2521 atccgcgggt tcctaccgtg agtccttcca gccgaaccag acataggcgc ccttgaggta
     2581 gagcgggcgg aaagcgggga gcggaaagcg cgccagcctt cgcgtcagca cggcggggat
     2641 ctccgagggc ggcgccttgc cggtcatcag ccgcgccacc gcgcggccgc tccagcttcc
     2701 catggccacg ccattgccgt gataggccag cgccgtccac acggtcttcg ccgcgtccag
     2761 cgcgccgaca tagggcacgc ggtcgaaggc gaggcagacg aagccgcgcc aatagtgggt
     2821 gatctccacg ccgcgccagg ccgggaagag gccatggaaa gcccgcgtca gcaccgcgcg
     2881 ataggcctcg ccgccgccgg cggaggcatc cgttccgccg cgcccgccga agaggaagcg
     2941 gccattgggc agcagccgga agtaatgcag caggttgcgc gagtcatagg ccatgagatc
     3001 cgaggtccag ccctgcgcgc ggcgttcggc ctccgagagc ggccgcgtca ccagcacgct
     3061 ggagagcgcc ggcatgatgc gtccggcatg gcgcggcgag acgtcttccg gcgtatagcc
     3121 gttggtcgcc accagcacgc gccgcgcggt cagcctgccg ccgggcgttg tcagcacatg
     3181 gcccgccgcc gtctcctccc agcgggtgag gcgcgatgat gcgtggattt tggcacccgc
     3241 cgcggctgcg gcgcgggcga ggccgcgcac atagttcagc ggatggatgc cgaagccgat
     3301 cttg
//
LOCUS       DBNGVR010000268         4571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5008807, whole genome shotgun sequence.
ACCESSION   DBNGVR010000268 DBNGVR010000000
VERSION     DBNGVR010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5008807"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="AC16A0_05730"
     CDS             complement(<1..613)
                     /locus_tag="AC16A0_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3104865.1"
                     /translation="MAKRPAPDYRDESVPALPETERGHPLVVFGDHRFCAGETTIVQR
                     MKGPHMHSQIELNFVLEGEMHYWFDGRALTVSAGRLALFWGMIPHQVTDIAEPTRFVV
                     LYVPMTVFLGLPALSRLREAIFRGAVVEALDIRPWDRDIFLRWREELLSGDPQIEQIV
                     RDELTARIRRLDREGWHDLRDVAPLAAAGLSHDTERALHVERMA"
     gene            780..2006
                     /locus_tag="AC16A0_05735"
     CDS             780..2006
                     /locus_tag="AC16A0_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3104866.1"
                     /translation="MTVFKGLTGLAFSFALTVSTALAGELVINSDQSDPAPKKAMEEL
                     IADFQAKNPDITVKWNNFDHEGYKSAIRNFLTADPPDVAAWYAGNRMAPFVKAGLFEP
                     VDDVWEANGFNDQLKSTAASMTIDGKKWGVPYTYYQWGIYYNKEAYKKAGVSEPKSWA
                     EFVANCEKFKAAGIDCLTTGTKALWPAAGIFDYLNLRTNGYEFHMDLANGKVPWTDPK
                     VKATFAEWAKIAPYTTANHAAIDWQDAAALLSQGKAAHYVMGNFAVPTFKDGGMTNET
                     LGFMVFPEITPGIPRAEEAPTDTFHIPSGAKNKEDAKKFLAYLGSAEAQTKMNATLNQ
                     LPVNNQSTVGDDPFIKAGFEMLSSAYALAQFFDRDAPADMAKAGMEGFQEFMVKPENV
                     DAILERLEKVRQKVYK"
     gene            2086..2979
                     /locus_tag="AC16A0_05740"
     CDS             2086..2979
                     /locus_tag="AC16A0_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3104867.1"
                     /translation="MSGSRSKRWNQRDIAPWLFLAPGLLLFLVYVIIPIFQSIWISFY
                     EWDGLGPKTWIGLDNSRELLDDDSFYTSLKNNIIWLVLYMLAIPAGLAVALFLNQTVA
                     GIRLYKSLFFFPFVISQVVVGLIFSWFYAPDFGLLPKIIGAITGTEVAILADERYATY
                     GIIAAGLWPQIAYCMILYLTGLNNINPEQVEAARMDNAKGWSMLWHIIIPQLRPATFI
                     AVVVTVIGALRSFDLISTMTVGGPYGSTRVLSYFMYEQALSEYGVAMGYGAAIAVVLF
                     AIMLVFITLFIVRMLAQERHG"
     gene            2979..3830
                     /locus_tag="AC16A0_05745"
     CDS             2979..3830
                     /locus_tag="AC16A0_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3104868.1"
                     /translation="MFPTPIARTSPWVQRSYKVLLPIALIVWLLPLIGIAVTSIRPSS
                     DLAAGNYFGMPSSFAGIENYTAVFKNSNIGYYILNSFRVTIPTVIGAVALSCLTGYAL
                     AVYKFKGNLLLFFMFIAGNFVPFQILAIPVRDLTLQLGLYNTTTGLVMFHVAFQTGFC
                     TLFMRNFIKGLPFALIESARVEGVSEWKIFWHIVMPLMRPAIAALSVLIFTFIWNDYF
                     WATIMAQSADVRPVTAGMNELKGQWKVAWELIAAASIIAALPPVAMFFMMQKHFIAGL
                     TLGATKG"
     gene            3834..4571
                     /locus_tag="AC16A0_05750"
                     /pseudo
     CDS             3834..4571
                     /locus_tag="AC16A0_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469112.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          845 a         1546 c         1336 g          844 t
ORIGIN      
        1 gcgccatgcg ctcgacatgg agcgcgcgtt ccgtgtcgtg gctcagtccg gccgcggcca
       61 gcggcgccac gtcgcgcagg tcgtgccagc cctcgcggtc gagacggcgt atgcgtgcgg
      121 tgagttcatc gcgcacgatc tgctcgatct gcggatcgcc cgacagcagc tcctcgcgcc
      181 agcgcaggaa gatgtcgcgg tcccaggggc ggatgtcgag cgcctcgacc accgcgccgc
      241 ggaagatcgc ctcgcgcagg cggctgaggg ccggaagacc caggaaaacg gtcatcggca
      301 catagagcac cacgaagcgg gtaggttccg cgatgtcggt cacctggtgc gggatcatgc
      361 cccagaacag cgccaggcga ccggccgaga cggtcagcgc gcggccgtcg aaccagtagt
      421 gcatctcgcc ttcgagcacg aagttgagtt cgatctggct gtgcatgtgc ggacccttca
      481 tccgctgcac gatggtggtc tcgccggcgc agaagcgatg atcgccgaag acgaccagcg
      541 gatggccgcg ctccgtctcg ggcagggcgg gcacgctctc gtcgcgatag tcgggggccg
      601 gacgcttcgc catgtgatct gacgacctta gaaaaccgga agtctgcaca atatctccgg
      661 tagaacctta cggttttgca gcgcatcgtc aagccataca cgcgaaccgg aaacaacaat
      721 aaccgggcac gtgcagaccc gccgccgcca tggtggctgc ggccaatggg aggaacaaca
      781 tgaccgtttt caagggcctc accgggctcg ccttcagctt tgccctgacc gtttcgaccg
      841 cgctcgccgg cgagctggtg atcaattcgg accagtccga tcccgcgccc aagaaggcga
      901 tggaggaact gatcgccgac ttccaggcca agaaccccga catcacggtg aagtggaaca
      961 acttcgacca cgagggctac aagtcggcca ttcgcaactt cctcaccgcc gatccgccgg
     1021 acgtggcggc ctggtatgcc ggtaaccgca tggcgccctt cgtcaaggcc ggcctcttcg
     1081 agccggtcga cgatgtctgg gaggccaatg gcttcaacga ccagctcaag tccaccgccg
     1141 cctcgatgac catcgacggc aagaagtggg gcgttcccta cacctattat cagtggggca
     1201 tctactacaa caaggaagcc tacaagaagg ccggcgtgag cgagcccaag agctgggccg
     1261 agttcgtcgc caactgcgag aagttcaagg cggccggcat cgactgcctc accaccggca
     1321 ccaaggccct gtggcccgcc gccggcatct tcgactacct gaatctccgc accaacggct
     1381 acgagttcca catggatctt gccaacggca aggtgccctg gaccgatccc aaggtgaagg
     1441 ccaccttcgc cgaatgggcc aagatcgcgc cctacaccac cgccaaccac gcggcgatcg
     1501 actggcagga tgccgcggca ctgctctcgc agggcaaggc tgcgcattat gtcatgggca
     1561 acttcgccgt gccgaccttc aaggacggcg gcatgaccaa cgagacgctc ggcttcatgg
     1621 tcttcccgga aatcacgccc ggaattccgc gcgccgagga agcgccgacc gataccttcc
     1681 acattccgtc gggcgccaag aacaaggaag acgcgaagaa gttcctcgcc tatctcggct
     1741 cggccgaggc ccagaccaag atgaacgcga cgctgaacca gcttccggtc aacaaccagt
     1801 ccaccgttgg cgacgatccc ttcatcaagg caggcttcga gatgctgtcc agcgcctatg
     1861 cgctggccca gttcttcgat cgcgacgcac cagccgacat ggccaaggcc ggcatggaag
     1921 gcttccagga gttcatggtg aagcccgaga acgtcgatgc cattctcgag cgcctcgaga
     1981 aggtccggca gaaggtctac aagtaatcct ccgctggggc cgggcatctt cggatgtccg
     2041 gcccactttc ttccagcctt gcgcctgcaa tcggggacgg ggatcatgag cggcagccgg
     2101 tcgaaacggt ggaatcagcg agacatcgcg ccctggcttt tcctggcgcc cggcctgctg
     2161 ctgttccttg tctatgtgat catcccgatc ttccagtcga tctggatcag cttctacgaa
     2221 tgggacgggc tcggccccaa gacctggatc ggcctcgaca actctcgcga gctgctcgac
     2281 gacgattcct tctatacctc gctcaagaac aacatcatct ggctcgttct ttacatgctg
     2341 gccatcccgg ccggcctcgc ggtcgcgctc ttcctcaacc agaccgtcgc cggcatcagg
     2401 ctctacaagt cgctgttctt ctttcccttc gtcatcagcc aggtggtcgt cggcctgatc
     2461 ttcagctggt tctacgcgcc cgacttcggc ctcctgccca agatcatcgg cgccatcacc
     2521 ggcacggaag tcgccatcct cgcggacgaa cgctacgcca cctatggcat catcgccgcc
     2581 ggcctctggc cgcagatcgc ctattgcatg atcctctacc tgaccgggct gaacaacatc
     2641 aatcccgagc aggtcgaagc cgcccgcatg gacaatgcca agggctggtc catgctctgg
     2701 cacatcatca tcccgcagct caggccggcc accttcatcg cggtcgtcgt caccgtgatc
     2761 ggcgcgctgc gctccttcga cctgatctcc accatgaccg tgggcggccc ctacggttcc
     2821 acccgcgtgc tgtcctactt catgtatgag caggccctgt cggagtatgg cgtggccatg
     2881 ggctatggcg cggccatcgc ggtggtgctt ttcgccatca tgctggtctt catcacgctc
     2941 ttcatcgtcc gcatgcttgc gcaggaaagg cacggctgat gtttccgacg cccattgccc
     3001 gcaccagccc ctgggtccag cgcagctaca aggtcctgct gcctatcgcg ctcatcgtct
     3061 ggctgctgcc gctgatcggc atcgccgtca cctcgatccg cccgtcgagc gacctggcgg
     3121 ccggaaacta cttcggcatg ccctccagct tcgccggcat cgagaactac acggccgtct
     3181 tcaagaacag caacatcggc tactacatcc tcaactcctt cagggtgacg atccccacgg
     3241 tgatcggcgc cgtggcgctc tcctgcctca ccggctatgc ccttgcggtc tacaagttca
     3301 agggcaacct gctgctgttc ttcatgttca tcgccggcaa tttcgtgccc ttccagatcc
     3361 tcgccattcc ggtgcgcgac ctcacgctgc agctgggcct gtacaacacc acgacggggc
     3421 tggtgatgtt ccacgtggcc ttccagacgg gcttctgcac gctcttcatg cgcaacttca
     3481 tcaagggcct gcccttcgcg ctgatcgagt cggcgcgcgt cgaaggggtg agcgagtgga
     3541 agatcttctg gcacatcgtc atgccgctga tgcggccggc cattgctgcg ctttcggtcc
     3601 tgatcttcac cttcatctgg aacgactatt tctgggccac catcatggcg cagtcggccg
     3661 atgtgcggcc ggtcaccgcc ggcatgaacg agttgaaggg ccagtggaag gtcgcctggg
     3721 agctgatcgc cgcagcatcg atcattgccg ccctgccgcc ggtcgcaatg ttcttcatga
     3781 tgcagaagca cttcatcgca ggcctcacgc tgggcgcgac aaagggatag accatggcga
     3841 ccgtcacatt gcgcaatgcc aggaagtcgt ttggcgacgt ccatatcatc aagggcgtcg
     3901 atctcgacat cgcggatggc gagttctgag tcttcgtcgg accctcgggc tgcggcaagt
     3961 ccacgctgct ccgcatggtc gccggtctcg aggagatcac atcgggatcg ctgcagatcg
     4021 gcggacgcga gatgcgggat gtggagcccg ccgaccgcgg cgtcgccatg gtgttccagt
     4081 cctatgcgct ctatccgcac atgacggtca aggacaacat cggcttcggc ctgcgcatga
     4141 ccgggcatga ccgtgccgac atcgagaagc gcacccagcg cgccgccgac atgctgcagc
     4201 tcgggccgct gctcgcccgc aagccgtcgc agctttccgg cggccagcgc cagcgcgtcg
     4261 ccatcgggcg cgccatcgtg cgcgaaccgg atgtctttct cttcgacgag ccgctgtcga
     4321 atctggatgc ggcgctgcgc gtgcagatgc gcatcgagat cggcaagctc cacagcgatc
     4381 tgaaatccac catgatctac gtgacccatg accaggtcga ggccatgacc atggccgaca
     4441 agatcgtcgt cctcaatgcc ggcatcatcg agcaggtcgg ttcgccgctc gaactctatc
     4501 accggccgcg caacaccttc gtcgccggct tcatcggcag cccgaagatg aacttcatca
     4561 aggccgaggc g
//
LOCUS       DBNGVR010000269         6770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3043532, whole genome shotgun sequence.
ACCESSION   DBNGVR010000269 DBNGVR010000000
VERSION     DBNGVR010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3043532"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..2252)
                     /locus_tag="AC16A0_05755"
     CDS             complement(198..2252)
                     /locus_tag="AC16A0_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3104869.1"
                     /translation="MDSTNGRRTPGSRCIALVGPYLSGKTTLLEAILFRTGAIPRQGR
                     IAEKSTVGDAAPEARAHGMSVELNAATTTFLGDSYTFIDCPGSIEFAQDSRAALLGCD
                     AAIVVCEADDKKAPALQLILKQLDEIGLPRIVFINKVDKSESSPRDVLSWLQPASSKP
                     LILRQLPIMKGTIVTGFVDLALERAFVYQEHAESKVVDMPADIAGIEKSERFAMLEKL
                     ADHDDALMEQLLSEMEPPRDVVFKDLSRELAEGLITPVLFGSAEHGNGVGRLLKALRH
                     EVPGVAQTVRRLGLKTGAGAVAQVLKTFHTAHGGKLSLVRLLAGEVADGTVFHGAQGQ
                     ESRTAGIFTLLGSTPHKIAKASAGDTVALGRLDAIATGETISTAKGHAPQLAEVPKAA
                     GVYGFAISVSDRKDEVKLTSAIAKLIEEDPSLSLDHNHDTHEMVLWGQGEMHLRVALE
                     RLQGKFGVHARSKPRRIAYKETIRKQAQIRGRHKKQSGGHGQFGDVVVEIAPQPRGSG
                     FRFSDTITGGVVPKQYIPAVEAGVREWMGHGPLGFPVVDFSVNLSDGSYHDVDSSEMA
                     FKTAARIAMSEGMPQCSPVLLEPIVEVEIHVPSEATSRINQIVTGHRGQLLGFDARDG
                     WPGWDTVRAHMPESEMGSLIIELRSATAGVGTFTYHFDHMAELTGRAADQVVNSRKAE
                     AA"
     gene            complement(2455..2940)
                     /locus_tag="AC16A0_05760"
     CDS             complement(2455..2940)
                     /locus_tag="AC16A0_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HIR3104870.1"
                     /translation="MKKCVAASLAVLGLLAIPASAADSDQPRKSGIKIGILTCRVDGG
                     VGWVIGSSKAVACVFKDNNGRKERYAGRIGKLGVDIGVTNQSSIAWAVFAPGKLKAGS
                     LKGSYTGASAEATIAVGLGANVLIGGFRKSINLQPISLQAQTGLNVAGGVASLYLERV
                     K"
     gene            complement(3068..3562)
                     /locus_tag="AC16A0_05765"
     CDS             complement(3068..3562)
                     /locus_tag="AC16A0_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HIR3104871.1"
                     /translation="MYEASVPVLTHFLKGLSAILKKAEAHCEARKIDPDAILLARLFP
                     DMFAFTRQVQLVTDFAKGAGARLAGIAVPSFADEEKTFAELQARIARTIDFLGTLRKE
                     QFADAATRTVTIKVAGNDMSFPGGEYFMGYAIPNFYFHLATAYNILRHNGIELGKRDF
                     MGRA"
     gene            complement(3578..4780)
                     /locus_tag="AC16A0_05770"
     CDS             complement(3578..4780)
                     /locus_tag="AC16A0_05770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3104872.1"
                     /translation="MGFISDALNRIQPSATIAISNKAMALKAEGRDVIGLAAGEPDFD
                     TPDNIKDAAIKAIRDGKTKYTPVDGIPELKKAICAKFKRENGLDYTPAQVTVGTGGKQ
                     VLFNALMATVNPGDEVIVPAPYWVSYPDIVMLAGGTPVFVDCKLEHGFKLQAADLERA
                     ITPKTKWLILNSPSNPSGAAYSRAELKAVTDVLMKHKHVWLLTDDMYEHLVYDDFAFT
                     TPAQIEPGLYDRTLTMNGVSKSYCMTGWRIGYGAGPEVLIKAMSKLQSQSTSNPSSIA
                     QWAAVEALNGPQGFIAANNKVFKERRDLVVSMLNQARGLSCATPEGAFYVYPSCAGTI
                     GKTAPSGNVIKTDEDFVTELLAAEGVAAVHGAAFGMSPFFRISYATGTDVLDDACRRI
                     QRFCGNLR"
     gene            4888..6054
                     /locus_tag="AC16A0_05775"
     CDS             4888..6054
                     /locus_tag="AC16A0_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3104873.1"
                     /translation="MSKPFLIAGGGIAGLAAALGLARGGRDIRLFEQAAAFEEVGAGL
                     QMSPNGVRALQELGAWDAVDPACVIPTEIHVRDGLTGRLLQRIRLGKGFETSFGAPYR
                     VCHRADLLEGLVQTARGRTGIVLETGRRVQSVESKVDGTALHLDGETVKGAAVICADG
                     IRSGLRKVVCGDVAPVDRGHVIFRSLIPLADVPSSIETDCVSLWLYPGGHAVHYAVSG
                     WRSFNIVVALDSDGIAASGWSDASDGSTVRQAFLDADDVLQDLLTRPAAWMRWRGADL
                     PGLSLWNKDNIVLTGDAAHATLPYLAQGAVMALEDAAVLGRETSMRARPAEAFAAFTQ
                     LRKPRTARLQLESRKLGAIYHARGLKSLLRNAALSVLGPDAALNRNRWIYDWRP"
     gene            6138..6668
                     /locus_tag="AC16A0_05780"
     CDS             6138..6668
                     /locus_tag="AC16A0_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3104874.1"
                     /translation="MRNTIIPLCFSALVMFASPVLAAGSGGSDGGSSPASQNCENGQV
                     WSKAKKKCVNASSGVLPDDELYEQGRALAMEGRYDWALQVLAAIENQNDPRVLNYTGY
                     SHRKAGRLDIGITYYRKALEIDPDFVLAREYLGEGYVAAGRVDLAMIELKEIGTRCGT
                     ACEEYKDLAAAISTAN"
BASE COUNT         1199 a         2143 c         2191 g         1237 t
ORIGIN      
        1 attgagattg cggggccgct tgttccgcca ccaaccggcg ctcgctagtc gtagcccgct
       61 ggctcattgg actgacgagt ctttcactgg atcttgtcca gtttgatgat tgaagcagct
      121 gttgtgaaat gtgattggag aaagggccgc ttgagcggcc ctttttgatt ttgatgctgc
      181 ctctcgcgcg cgactgacta ggccgcctcc gccttgcgcg agttcaccac ctggtccgcc
      241 gctcgccccg tcagttcggc catgtggtcg aaatgatagg tgaaggtgcc aacacctgcg
      301 gtggccgaac gcagctcgat gatcagcgag cccatttccg actcgggcat atgcgctctg
      361 acggtgtccc agcccggcca gccgtcgcgg gcgtcgaaac ccagaagctg tccgcgatgg
      421 ccggttacga tctggttgat ccgcgacgtg gcctcgctcg gcacatggat ctcaacttcc
      481 acgatgggtt ccagcaggac cggcgaacat tgcggcatgc cctcgctcat ggcgatgcgc
      541 gccgccgtct tgaaggccat ctcggaggag tcgacgtcat ggtaggaacc atccgaaaga
      601 ttgaccgaaa aatcgaccac cgggaagccg agcgggccat ggcccatcca ttcgcgcacg
      661 cccgcctcga cggcgggaat gtattgcttc ggcaccacgc cgccggtgat ggtatcgctg
      721 aaccggaagc cgctgccgcg cggttgcggc gcgatctcga ctacgacatc gccgaactgg
      781 ccatgcccgc cggactgctt cttgtggcgg ccgcggatct gcgcctgctt gcggatcgtc
      841 tccttgtagg caatgcggcg gggcttcgag cgggcatgaa cgccgaactt gccctgcagc
      901 cgctccagcg ccacccgaag atgcatctcg ccctgccccc acaggaccat ctcgtgggtg
      961 tcatggttgt ggtcgagcga cagcgacgga tcctcctcga tcagcttggc tatggcgctg
     1021 gtgagcttca cctcgtcctt gcggtccgac accgagatgg cgaagccata gacgcccgca
     1081 gccttcggca cctcggcaag ctgcggagcg tggcccttgg ccgtcgagat ggtctcgccc
     1141 gtggcaatgg catcgagccg gccgagcgcc accgtgtcgc cggcactggc cttggcgatc
     1201 ttgtgaggcg tcgagcccag cagggtgaag atgccggcgg tacgggattc ctgcccctgc
     1261 gcgccatgga aaaccgtgcc gtcggcgacc tcgcccgcca gcagccggac cagcgacagc
     1321 tttccgccat gggcggtgtg gaaggtcttg agcacctgcg caaccgcgcc ggccccagtc
     1381 ttgaggccga gccgtctgac cgtctgggcc acgccgggaa cttcatggcg cagcgccttc
     1441 agcagccgac ccacgccgtt gccatgctca gccgagccga agagcacggg cgtaatcagg
     1501 ccctccgcca gttcgcgcga caggtccttg aacacgacat cgcgtggcgg ctccatctcc
     1561 gacagcagct gttccatcag tgcgtcgtcg tggtccgcaa gcttctccag catggcgaag
     1621 cgctcgctct tctcgatgcc ggcaatgtcg gcaggcatgt cgacaacctt gctttcggca
     1681 tgttcctggt agacgaaggc acgctccagc gcgagatcga cgaaccccgt cacgatggtg
     1741 cccttcatga tcggcagctg gcgtaggatc agcggcttgc tgctggccgg ctgcagccac
     1801 gaaagaacgt cgcgggggct cgactcggac ttgtcgacct tgttgatgaa gacgatgcgc
     1861 gggaggccga tttcgtcgag ttgcttgaga atgagctgga gggcgggcgc cttcttgtca
     1921 tcggcttcgc agacgacgat ggcggcatcg cagcccagca gcgcggcgcg ggagtcctgc
     1981 gcgaattcga tggagcctgg acagtcgatg aaggtgtagc tgtcgccgag aaaggtggtg
     2041 gtcgccgcgt tgagctcgac gctcatgccg tgggcgcggg cttccggagc ggcgtcgcca
     2101 acggtggatt tctcggcgat gcggccctgg cgcggaatgg cgccggtgcg aaacagaatg
     2161 gcttcgagaa gcgtggtctt gccgctgaga taggggccca cgagtgcgat gcaccgggag
     2221 cccggcgttc ttctgccgtt tgtcgagtcc atggccaatc ctcctttgac tgctgaaaaa
     2281 gcgccgaagg accgcccttc tttctatctg cgtgaacacg ccggggcggt tccacacgcg
     2341 aaagcttgac tccgtttcaa aaagtcagca agcgaaatcc ggaactgatt ggcagacaag
     2401 gaaaaagggc cgcgacgcgc gcggccccct cgaacttgcc atcggatgcg cgggttactt
     2461 cacacgctcg aggtagaggc tggccacgcc gccagccacg ttcagtccgg tctgcgcctg
     2521 caggctgatg ggctgcaggt tgatcgactt gcggaagccg ccgatcagca catttgcgcc
     2581 gagtccgacc gctatggtgg cttcggccga tgcgccggta taggacccct tcagggaacc
     2641 cgccttgagc ttgccgggcg cgaagacggc ccaggcgatc gagctctggt ttgtcacacc
     2701 gatgtcgacg ccgagcttgc caatgcgacc tgcatatcgc tccttgcggc cattgttgtc
     2761 cttgaacacg caggcgacgg ccttggacga accgatgacc cagcccacac cgccgtcaac
     2821 ccggcaggtg agaatgccga tcttgatgcc gctcttgcgc ggctgatcgg aatccgccgc
     2881 ggaggccgga atggccagaa ggccgaggac ggcaagcgag gcggcaacac acttcttcat
     2941 aatcactggt tccttgttgg tttcagcaac ccgttatggc gcggaatgtg tcggcaattg
     3001 gactgcccag acaccgggtc gcgaggcaag ccggaaggca gacttgccct ttggaaacct
     3061 caacgcgtca agcgcggccc atgaaatcgc gcttgcccag ctcgatgcca ttgtggcgca
     3121 ggatgttgta ggctgtcgcg aggtggaagt agaaattggg tatggcgtag cccatgaagt
     3181 actcgccgcc cggaaagctc atatcgttgc cggccacctt gatggtaacg gtacgcgtgg
     3241 cggcgtcggc gaactgctcc ttgcgcaggg tccccagaaa atcgatcgtc ctggcgatgc
     3301 gggcctgcag ttcggcgaag gtcttttcct catcggcgaa actcggcacc gcgataccgg
     3361 ccaacctcgc gccggcgccc ttggcgaagt cggtgaccag ctgcacctgg cgggtgaagg
     3421 cgaacatgtc ggggaacagc cgcgccagga gaatcgcgtc gggatcgatc ttgcgcgctt
     3481 cgcaatgcgc ctcggccttc ttgaggatgg ccgagaggcc cttcaggaaa tgggtgagaa
     3541 ccggaacgga agcttcgtac atcattgagg tttttcctta tctgagattg ccgcagaagc
     3601 gctggatgcg cctgcatgcg tcgtccagca catcggtgcc ggtggcatag gagatgcgga
     3661 agaagggcga catgccgaag gccgcgccat gcactgccgc cacgccttcg gcggcgagca
     3721 gctcggtcac gaagtcctca tccgtcttga tcacattgcc cgaaggcgcg gtcttgccga
     3781 tggtgccggc gcaggaggga tagacataga aggcgccttc cggcgtggcg caggaaaggc
     3841 ccctggcctg gttcagcatc gacacgacga ggtcccggcg ctccttgaag accttgttgt
     3901 tggcggcgat gaaaccctgc ggcccgttga gcgcctcgac ggccgcccat tgcgcgatgg
     3961 aggacggatt cgatgtcgac tgcgactgca gcttggacat ggccttgatc agcacttccg
     4021 gcccggcgcc atagccgatg cgccagccgg tcatgcagta ggacttggac acgccgttca
     4081 tggtgagcgt gcggtcgtag agccccggct cgatctgggc gggcgtggtg aaggcgaagt
     4141 cgtcgtagac gagatgctcg tacatgtcgt cggtcagcag ccagacatgc ttgtgcttca
     4201 tcagtacgtc ggtgacggcc ttgagctcag cgcgcgaata ggccgccccc gaggggttcg
     4261 acggagagtt gaggatcagc cacttggttt tcggcgtgat cgccctttcg agatccgcag
     4321 cttgcagctt gaagccgtgc tccagcttgc agtccacaaa caccggcgta ccgccggcca
     4381 gcatgacgat gtcgggatag ctcacccagt agggcgccgg cacgatcacc tcgtcgcccg
     4441 gattgacggt agccatcagg gcattgaaca ggacctgctt gccgccggtg ccaaccgtga
     4501 cctgcgcggg cgtgtagtcc aggccgttct cgcgcttgaa cttggcgcag atcgctttct
     4561 tgagctccgg aatgccgtcg accggcgtgt acttggtctt gccgtcgcgg atcgccttga
     4621 tggcggcgtc cttgatgttg tccggcgtat cgaagtccgg ctcgccggca gcaagcccga
     4681 tgacgtcacg gccctcggcc ttgagcgcca tggccttgtt ggaaatggcg atggtggcag
     4741 acggctggat gcggttgagc gcgtcggaga tgaaacccat ggcaggaaac ctttcgaaag
     4801 ccgcgcctgt ctaggcccac gggctgcggc gggcaagcct caatccgccg catgacctga
     4861 gccgcccgac tgtggcaatc tgaccaaatg agcaagcctt tcctgattgc gggcggcggc
     4921 attgccggtc tcgcggcggc actgggcctc gcccgcggcg ggcgcgacat ccggcttttc
     4981 gaacaggcag ccgccttcga ggaagtgggc gccggactgc agatgtcacc caatggggtt
     5041 cgggccctcc aggagctggg ggcttgggat gcggtggacc ccgcctgcgt cattcccacc
     5101 gagatccacg tgcgcgacgg cctcactggc cggctgctcc agcgcatccg gctgggcaag
     5161 ggtttcgaga ccagcttcgg cgcgccctac cgcgtctgcc atcgcgccga tctgctggag
     5221 ggcctcgtac agaccgccag gggaagaacg gggatcgtcc tcgaaaccgg caggcgggta
     5281 cagtcggtag aatcgaaggt cgacggtaca gcacttcacc tcgacggcga gacggtgaag
     5341 ggcgcggctg tcatctgtgc cgacgggata cgctctggcc tgcgcaaagt ggtctgcggc
     5401 gacgtcgctc ccgtcgaccg cggacatgtc atcttccgca gtctcatccc tcttgccgat
     5461 gtaccatcct cgatcgagac cgactgcgtg agcctgtggc tctatcccgg cggccacgcg
     5521 gttcactacg ccgtgagcgg ctggcgcagt ttcaacatcg tggtcgcgct ggactccgac
     5581 gggatcgccg cgtcagggtg gtccgatgcc tccgacggca gcacggtacg gcaagccttc
     5641 cttgacgcag acgacgtgct gcaggacctt ctgacccggc ccgctgcgtg gatgcggtgg
     5701 cggggcgccg acctccccgg actgtcgctc tggaacaagg acaacattgt gctgaccggc
     5761 gacgccgcgc atgccaccct gccctacctc gcgcaggggg ccgtgatggc actggaagac
     5821 gctgccgtgc ttggaaggga aacatccatg cgcgccaggc cggcagaggc cttcgcggcc
     5881 ttcactcagc tccgcaaacc ccggacggcc cgacttcaac tggagtcccg aaagctgggc
     5941 gcaatctacc acgcacgcgg attgaaatcg ctcctgcgca acgccgcgct gtcggtcctg
     6001 ggcccggatg cggcattgaa ccgcaatcgc tggatctatg actggcggcc ctagccccgc
     6061 gatgccgttt ccgtgattac caccatcgat ccacggcgtg ctagtctgcg taaagacggc
     6121 aaacggagaa cagaaccatg cgcaacacga tcattccctt gtgtttctca gccctcgtca
     6181 tgttcgcctc gcctgtcctg gcagccggca gcggcggcag cgacggcggc tcttcgccag
     6241 cgtcgcagaa ctgcgaaaat ggacaggtct ggagcaaggc caaaaagaaa tgcgtgaatg
     6301 catcttccgg cgtgcttcct gacgacgagc tctacgagca gggccgcgcc ctcgccatgg
     6361 aaggccgcta tgactgggcg ctccaggtgc tcgccgccat cgagaaccag aacgatccgc
     6421 gcgtgctgaa ctacacggga tacagccacc gcaaggctgg ccggctcgac atcggcatca
     6481 cctactaccg caaggccctg gagatcgatc ccgacttcgt gctcgcccgc gagtacctgg
     6541 gcgaaggcta tgtggccgcc ggccgcgtcg atctcgcgat gatcgagctg aaggagatcg
     6601 gcacacgctg cggcaccgcc tgcgaagaat acaaggatct ggcggctgcc atctcgaccg
     6661 ccaactgagt gacgatcaga ccgggggcac cagccacctg acggtggcct gcccccagtc
     6721 tccgagaccg agacgctgcg caagagctga cgtgattgcg gtcagctcgt
//
LOCUS       DBNGVR010000270         3379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5009279, whole genome shotgun sequence.
ACCESSION   DBNGVR010000270 DBNGVR010000000
VERSION     DBNGVR010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5009279"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..739)
                     /locus_tag="AC16A0_05785"
     CDS             complement(23..739)
                     /locus_tag="AC16A0_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104875.1"
                     /translation="MPIELAQTIALIDHHCHGITSKDLDYVNFQKFISESYRPPPEGT
                     SDFQKPVGLAVRRFCAPVLDLEKGVSGEDYVRRRQELGPDEVNRRLMRACGLSHLLID
                     TGHRASIILDVPAMAEVTAVPAREVVRIEALAEEIMRAGVSADAFPNVFADTLRKRSR
                     NAVGLKTIVAYRTTFAIDYSPPSHEAVSQAAGQWMRRAEVSGKWRLDDATIIRHLPEA
                     SARFIHWPAACETASCDGRL"
     gene            complement(729..2048)
                     /locus_tag="AC16A0_05790"
     CDS             complement(729..2048)
                     /locus_tag="AC16A0_05790"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3104876.1"
                     /translation="MSTPSTDVAYLCWSDYVGVTRCRGVPANDLSKRMAKGLGWAVAG
                     QALTPFDDIADNPWGPMMEVRQTPNAETEVRLDLWPGTPAFHILMCDSNVNDGTNWEC
                     CTRGFMKKALGDFEAETGLRFTAAFEHEFLLSGPDLPPATPFSLEAMRVAAGFTGALA
                     EALALAGLEPQTIEPEYGVNQYEVSTAPAAGTMAGDRAVLTREVIREVARRHGYRASF
                     TPKPAPHAVGNGAHVHFSFLDHEGRNASYDSHGTAEAAQVAQHFVAGLVRHLPDICAL
                     VAGSPVSYYRLGPHHWSCGYASFGVQNREAAVRICPSPESDPSRRQSGFNMELRAPDA
                     TASPYMVVGAILRAGLEGIKAKLPLPTPIDRDPSELSPKEMEALGVVALPGSLAEALA
                     RLEASPVVRQWMSPTFLSSYVSVKRKEIAMMDGLSPEEICARYRDAY"
     gene            complement(2069..2764)
                     /locus_tag="AC16A0_05795"
     CDS             complement(2069..2764)
                     /locus_tag="AC16A0_05795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3104877.1"
                     /translation="MRLPSIPAVDAAGGAYRERPIELAKTALLSIDMQNAEWSPERMA
                     RARVAGSPEAPYLAIMEKIEKQLIPNQRRLQAAARKAGIEVIYTTIESYTLDGRDRSL
                     DHKISELFCPKGSWEAKVIAEVGPEPNDIVIPKTASGIFNSTNVEYVLRNLGIEYLVI
                     YGICTDQCVECAIRDAADRGFMVTQIEDACAAVEQRRHDVSIDQMRGHYCRTRTTDEM
                     IAEIEALSQSAAA"
     gene            complement(2778..>3379)
                     /locus_tag="AC16A0_05800"
     CDS             complement(2778..>3379)
                     /locus_tag="AC16A0_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR3104878.1"
                     /translation="HQSKPAKRSNPDRLLVENMTESLRKLLEPPTQRALEKAADLLGN
                     ATARIYLLGGRWSSAAAQYAAFQLTSLRGEIHVLAPQPSGLVEDRIADFTKRDVLVAY
                     DFRRYQPETIAFCQAAKQRGVKLVLFTDLDLSPVADIADVVVPVTVECTSPLETLAVA
                     IAATDAMLARLVRRLGTQASRRMAMLENLRRDASIPKLS"
BASE COUNT          575 a         1067 c         1115 g          622 t
ORIGIN      
        1 accggaccac cttcgccatc gactacagcc gcccgtcgca tgaggcggtc tcgcaggccg
       61 cgggccaatg gatgaagcgc gcggaggctt caggcaaatg gcggattatc gtggcatcgt
      121 ccaggcgcca cttgcccgaa acctcggcac gtctcatcca ttgtccggct gcctggctta
      181 ccgcctcgtg cgacggcggg ctgtagtcga tggcgaaggt cgtgcggtag gcgacgatgg
      241 tcttgagccc aacggcgttg cgtgaccgtt tgcgcagcgt gtcggcaaag acattgggaa
      301 acgcgtcggc cgagacgcct gcgcgcatga tctcttcggc cagcgcttcg atgcggacga
      361 cttcgcgtgc cgggacggcg gtcacctctg ccattgcggg aacgtcgaga atgatgctgg
      421 cgcggtggcc cgtgtcgatc agcagatgcg acaggccgca ggcgcgcatc agacgccggt
      481 tgacttcgtc cggtcccagt tcctgacgtc gccgcacgta atcctcgccg gagacgccct
      541 tctcgaggtc cagcaccggc gcgcagaagc gccgaacggc aagtccgacg ggcttctgga
      601 agtcgctcgt tccctccggc ggcggacgat agctttcgga gatgaacttc tggaagttca
      661 catagtcgag gtctttcgac gtgatgccgt ggcaatgatg gtcgatgagc gcaatggtct
      721 gtgccagttc aataggcatc gcgatacctc gcacagatct cttccgggct cagtccgtcc
      781 atcatggcga tctccttgcg cttcaccgag acataggatg acaggaaggt gggcgacatc
      841 cactggcgga ccacaggact ggcttccagc cgcgccagcg cctccgcgag gcttccgggc
      901 agggccacca cgccgagcgc ttccatttct ttcggggaca gctcgctggg atcgcgatcg
      961 atgggggtcg gcagggggag ctttgccttt atgccttcga gtcccgcccg caggatggca
     1021 ccgacaacca tgtagggact cgccgtggcg tcgggcgcac ggagttccat gttgaagccg
     1081 gactgccgcc gcgacggatc ggactccggc gagggacaga tgcgaacggc agcctcgcga
     1141 ttctgcacgc cgaagcttgc atagccgcag gaccagtgat gagggccgag ccggtagtag
     1201 gacaccgggc tgcccgccac caatgcgcag atgtccggca ggtgccgtac caggcccgcc
     1261 acgaagtgct gcgccacctg cgcggcttcg gccgtgccgt ggctgtcgta ggaggcgttc
     1321 cggccctcgt gatcgaggaa gctgaaatgg acgtgcgcgc cgttgcccac cgcatgtggc
     1381 gcgggcttgg gcgtgaagga ggcgcgatag ccgtgacgcc gcgcgacctc gcggatcacc
     1441 tcgcgggtca gcacggcacg gtcgccggcc atggtgcccg cggcaggcgc ggtcgagacc
     1501 tcgtactggt tgacaccgta ctccggttcg atcgtctggg gctcgagtcc ggccagcgcc
     1561 agggcttccg ccagtgcgcc ggtgaagccg gcggcaacgc gcatggcttc gagcgagaac
     1621 ggcgtggcgg ggggaaggtc aggaccggaa agcagaaact cgtgctcgaa ggcagccgtg
     1681 aagcgcaggc ccgtctctgc ctcgaaatcg ccgagcgcct tcttcatgaa gccgcgcgtg
     1741 cagcattccc agttcgtgcc gtcgttgacg ttcgagtcgc acatcaggat gtggaaggcc
     1801 ggcgtgcccg gccacaggtc aagccgcacc tccgtctcgg catttggcgt ctgccgcacc
     1861 tccatcatcg gaccccaggg gttgtcggca atgtcgtcga agggcgtcag cgcctggccg
     1921 gccacggccc atcccaggcc cttcgccatg cgcttcgaca gatcgttcgc aggtacgccg
     1981 cggcagcgcg taacgcccac atagtcgctc cagcagagat aggcgacgtc ggttgatggt
     2041 gtactcatcc tggtctccaa gactccggtc aggcggcggc cgactgtgac agcgcctcga
     2101 tctcggcaat catttcgtcg gtcgtccgcg tccggcagta atggccgcgc atctggtcga
     2161 tcgaaacgtc atggcggcgc tgctcgacgg cagcgcaggc atcctcgatc tgagtcacca
     2221 tgaagccgcg gtcggcggca tcgcggatgg cgcattccac gcattgatcc gtgcagatac
     2281 cgtagatgac aaggtattcg atgccgaggt ttcgcaacac atactcgacg ttggtggagt
     2341 tgaagattcc ggaagctgtc ttggggatga cgatatcgtt gggctcgggt cccacctcgg
     2401 cgatcacctt cgcctcccac gagcccttcg ggcagaacag ctccgagatc ttgtgatcga
     2461 ggctgcggtc gcgtccatcg agcgtgtagc tctcgatcgt cgtgtagatg acctcgatgc
     2521 ccgccttgcg ggccgccgcc tgaagtcgcc tctggttggg gatcagctgc ttctcgatct
     2581 tctccatgat cgccagataa ggcgcctccg gcgaacccgc tacgcgtgcc ctggccatac
     2641 gttccggact ccactcggca ttctgcatgt cgatcgaaag gagcgccgtc ttggccagtt
     2701 cgattgggcg ttcgcgatag gctccacccg cggcgtcgac ggcaggaatc gagggaagtc
     2761 gcatgtgtct ggtcctctca ggaaagtttc gggatactag cgtctcgccg caggttttcc
     2821 agcatggcca tgcgccggct ggcctgggtg cccagtcttc tgacgagtcg cgcaagcatt
     2881 gcatcggtcg cggcgatggc caccgccagc gtttcaagcg gcgaggtgca ctcgacggtg
     2941 actggcacga caacatcggc aatgtcggca acaggcgaga gatcgagatc ggtgaacagc
     3001 acgagtttca cgccacgctg cttcgcggcc tggcagaagg cgatggtctc gggctggtag
     3061 cgccggaaat cataggccac caatacgtcg cgcttcgtga agtcggcgat gcggtcctcg
     3121 acgagaccgg agggctgggg cgccagcacg tggatctcgc cacgcaggct cgtgagctga
     3181 aacgcggcgt attgcgcggc ggccgaactc cagcgcccgc ccaggagata aatgcgcgcc
     3241 gtggcattcc ccaacaggtc agccgccttt tccagcgcgc gctgtgtggg tggctcgagc
     3301 agtttcctga gcgactccgt catgttctcc accagcaggc gatccggatt cgaacgcttt
     3361 gccggcttgc tctgatgct
//
LOCUS       DBNGVR010000271         3562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1475090, whole genome shotgun sequence.
ACCESSION   DBNGVR010000271 DBNGVR010000000
VERSION     DBNGVR010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1475090"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="AC16A0_05805"
     CDS             complement(<1..938)
                     /locus_tag="AC16A0_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3104879.1"
                     /translation="MNRADCEALDRADPLAPKRALFAIPDGMNYLDGNSLGVLPKGVA
                     ERVGDTVTQHWGETLIRSWNEHGWFALPRKVGDRIAKLVGAAPGTVIAGDTISINLFK
                     LLGAALKLRPDRKVILSDSGNFPSDLYVAQGLRDLLGGGHVLRIVEPDAVVQAIDDTV
                     AVVMLTEVDYRTARLHDMRAVTAAAHAAGALTIWDLAHSAGATPVDLASANADFALGC
                     TYKYLNGGPGAPAFLYVRPDLQDTVRPPLAGWWGHAAPFAFELDYRPAPGIARNQCGT
                     QPILSLAALDAALDVWDGVDLQELRRKSMALCKLFI"
     gene            complement(995..1927)
                     /locus_tag="AC16A0_05810"
     CDS             complement(995..1927)
                     /locus_tag="AC16A0_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3104880.1"
                     /translation="MDDQGISQARDVLSRIPQFWHPDDDAVKITRLGGLTNLVFRVDH
                     GGEQYVLRIPGKGTEEYINRANEAQAAREAAKAGVSPDVLHFDLQTGVMITRLVDHAV
                     TMTPEGFKSIAGAPARAGAVFRKLHTSGARFNFRFELFSMIDDYLKILATKDISLPDG
                     YHDVVKEARSVRAALAAHPLPLVACHCDPLCENFLDVGNRMWLVDWEYSGMNDPMWDL
                     GDLAVEGQFDSVQEEDMIRAYFGGEPTPAERGRIVIYKAMCDLLWTLWGLIQHANRNP
                     ADDFWAYSVNRFNRCKALMATAEFPRHLAAVARG"
     gene            complement(1929..>3562)
                     /locus_tag="AC16A0_05815"
     CDS             complement(1929..>3562)
                     /locus_tag="AC16A0_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR3104881.1"
                     /translation="FAIGGWEDGSVGFHRGLPPFSFGRELLPLNMERLELFAIPAASR
                     LPILNETGIQTVINGPIPVSADGEPIMGLAPGYSNFYVACGFTAGIAASGGAGLAMSN
                     LILHGDAGMDLWPFDARRFSALQAQPRYLEERAIEAYGAYYKIHWPGEEAQVGRGLRR
                     SPLHDHLTARNAVFGSKMGWERPNWFAPQGAAPADRPSFEDKPGWFDGVAAEHLAIRT
                     RAALIDQTSFAKFEIAGSGTEAALQRIAANDLSGPPGKAVYTQLCNARGGIEADVTLV
                     HEAADRFMLVTGSGFGLRDSGWVASHLPDGVTIREITSSLAVINLCGPRSRDILQAVT
                     DDDVSNAAFPFLAARRIDVGLARALAVRIGYVGELGYELYIPQEFAAHVYETLWSAGE
                     SHGIANAGYRAIDSCRLEKGYLYWSGDITPDYNPYEAGLGFCVALDKGDFIGRDALAK
                     IRAEGVTRKLCSFSVEGFAPLLGGEAILHDGKVVGSTSSAGFGHTLGMTIAFGYLPAE
                     IAGETRFVIEAFGKGYPATRGPRCLYDAKMERLRG"
BASE COUNT          651 a         1177 c         1148 g          586 t
ORIGIN      
        1 tcgatgaaga gcttgcacag cgccatggac ttgcggcgca gctcttgcag gtcgacgccg
       61 tcccacacgt cgagcgccgc gtcgagcgcg gcgagcgaca ggatgggctg cgtgccacac
      121 tggttacggg cgatgccagg cgcggggcga taatcaagct cgaatgcaaa gggcgcggca
      181 tggccccacc aacccgccag cggcggccgt accgtgtcct gcaggtccgg gcgcacataa
      241 aggaaggcgg gcgcgccagg ccctccgttg aggtatttgt aggtgcagcc caacgcgaag
      301 tcggcattgg cactggcgag atcgacgggc gtggcgccgg ccgaatgcgc aagatcccag
      361 atggtcagtg cgccggcggc atgggctgcg gcggtcacgg ccctcatgtc atgcagccgc
      421 gcggtccggt agtccacttc cgtcagcatg acgacggcca ccgtgtcgtc gatggcttgc
      481 accacggcgt ccggctccac gatccgcaga acatggccac cacccagcag gtcgcgcagg
      541 ccttgcgcca catagaggtc ggacgggaaa ttgccggagt cggagaggat caccttgcgg
      601 tccggccgca gcttcagcgc cgcgccgagc agcttgaaca ggttgatcga gatcgtatcg
      661 cccgcaatca ccgttcccgg cgccgcaccc acgagcttcg cgatcctgtc gcccaccttc
      721 cgcggcagcg cgaaccagcc atgttcgttc cacgatctga tgagcgtttc gccccagtgc
      781 tgcgtgacgg tgtcgccaac ccgttctgcg acgcctttgg gcaggacgcc aagcgaattg
      841 ccatcgaggt agttcatccc atcggggatc gcgaagagcg ctcgcttcgg cgccagcgga
      901 tcggcgcggt cgagggcctc gcagtcggcg cggttcatgc atcactccgt ctcgtgcgcc
      961 gccgttcttg catgccggac gcggcgactc aacctcagcc gcgtgccacc gcggcaagat
     1021 gcctggggaa ctccgccgtc gccatcaggg ccttgcagcg gttgaagcgg ttcaccgaat
     1081 aggcccagaa gtcgtccgcc ggattccggt tggcatgctg gatcaggccc cagagggtcc
     1141 agagcaggtc gcacatggcc ttgtagatga cgatgcggcc ccgctcggcg ggcgtcggtt
     1201 cgccgccgaa ataggcgcgg atcatgtctt cttcctgcac cgaatcgaac tggccttcca
     1261 cggcaagatc gccgagatcc cacatgggat cgttcatgcc ggaatactcc cagtccacca
     1321 gccacatgcg gtttccgaca tcgagaaaat tctcgcagag cggatcgcaa tggcaggcca
     1381 ccagtggcag cggatgcgca gccagggcgg cccgcaccga ccgggcctcc ttcaccacat
     1441 cgtggtagcc atcgggaagc gagatgtcct tggtcgcgag aatcttcaga tagtcgtcga
     1501 tcatgctgaa gagttcgaag cggaagttga acctcgcacc tgatgtatgg agcttgcgga
     1561 agaccgcgcc ggcccgcgcc ggcgcaccgg caatcgactt gaatccctct ggagtcatgg
     1621 tcacagcgtg atcgacgaga cgggtgatca tcacgccggt ttgcagatca aagtgcagga
     1681 catcgggact gaccccggcc ttcgccgcct cgcgcgccgc ctgcgcctcg tttgcgcggt
     1741 tgatgtattc ctcggtgccc ttgccgggaa tccgcagcac gtattgctcg ccgccatgat
     1801 cgacgcggaa aacgagattg gtcagcccgc ccagacgggt gatcttcacc gcatcgtcgt
     1861 caggatgcca gaactgcgga atccggctca gcacgtcccg cgcctgcgaa atgccctggt
     1921 cgtccatcct accctctcag ccgttccatc ttcgcgtcat agaggcagcg cgggccgcgc
     1981 gtggccgggt agcccttgcc gaaggcttcg atcacgaagc gcgtctcgcc tgcgatctcg
     2041 gcaggaagat aaccgaatgc aatggtcatg ccgagcgtgt ggccaaagcc cgcgctgctg
     2101 gtcgagccca ccaccttgcc gtcatgcagg atcgcctcgc cgcccagcaa aggtgcgaag
     2161 ccttcgaccg agaaggagca gagcttccgc gtgacgcctt cggccctgat cttcgccagc
     2221 gcgtcgcggc cgatgaaatc gcccttgtcg agcgccacgc agaagccgag ccccgcctcg
     2281 tagggattgt agtcgggcgt gatgtcgccg gaccaataga gatagccttt ctccaggcgg
     2341 caggagtcga tggcgcggta gccggcattg gcgatgccat gcgactcgcc tgcggaccag
     2401 agtgtctcat agacatgggc ggcgaactcc tgcgggatgt agagttcata tcccagttcc
     2461 cccacatagc cgatgcgcac ggcaagcgcg cgcgccaggc ccacatcgat ccgccgggcc
     2521 gcgagaaagg gaaaggccgc attcgacacg tcgtcgtcgg tcactgcctg gagaatatcg
     2581 cgcgaccgcg gaccgcagag gttgatcacg gcgaggctgc tggtgatctc gcgaatcgtg
     2641 acgccgtccg gaagatggct ggcaacccaa ccggagtcgc gcagaccgaa gccggacccg
     2701 gtgaccagca tgaagcggtc ggctgcctca tggaccaacg tgacgtccgc ctcgatgcca
     2761 ccgcgcgcat tgcagagctg cgtgtagacc gcctttccgg gaggtccgga aagatcattg
     2821 gccgcgatgc gctgcagggc ggcctcggtg ccggaccccg cgatctcgaa cttggcgaag
     2881 gaggtctggt cgatcagcgc agcgcgcgtg cggatggcaa gatgttccgc cgccacgcca
     2941 tcgaaccacc ccggcttgtc ctcgaaactg ggccggtcgg ccggtgctgc accttgcggc
     3001 gcgaaccagt taggccgctc ccagcccatc ttggatccga agacggcatt gcgtgccgta
     3061 aggtggtcat gcaggggcga gcgccgcaat ccacgtccga cctgcgcctc ctcgccgggc
     3121 cagtggatct tgtagtaggc gccataggcc tcgatggccc gttcttccag atagcgcggc
     3181 tgcgcctgca gcgccgagaa gcgccgcgca tcgaagggcc agagatccat cccggcgtcc
     3241 ccatgcagga tgaggttcga catggcgagc cccgccccgc ccgaggccgc aatccccgcc
     3301 gtgaagccgc aggccacgta gaaattggag taacccggcg cgagacccat gataggctcg
     3361 ccatcggccg agaccgggat cggcccattg atcaccgtct ggattcccgt ttcgttcagg
     3421 atcgggagtc ggcttgccgc cggaatggcg aagagctcga gccgctccat gttgagcggg
     3481 aggagttcgc gcccgaagct gaagggcgga agcccgcggt gaaaaccaac agacccgtcc
     3541 tcccagccac cgatagcgaa gg
//
LOCUS       DBNGVR010000272         3374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1082150, whole genome shotgun sequence.
ACCESSION   DBNGVR010000272 DBNGVR010000000
VERSION     DBNGVR010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1082150"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..849)
                     /locus_tag="AC16A0_05820"
     CDS             complement(34..849)
                     /locus_tag="AC16A0_05820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR3104882.1"
                     /translation="MAGTDKTRNQVLGPAPVTVLVNPQLGENIGTAARAMANFGLHGL
                     RLVDPRDGWPNEKALTASSGANWIIEAATVHPDLPSALADMNYVYATTARPRGMIKEV
                     ITPEEAGHDMRRRIARGEKVAVLFGRERWGLNNDEVSLADVIVTAPVNPAFASINIAQ
                     AVLLVGYEWYKEQAETLGQQTPELPALEGPGLQTPDTRPATKEEFYGFLDHLERELDT
                     AGFFKTGDKKPGMLRNIRNLFARAELTEQEVRSLRGIVASLTRAHERRREPKG"
     gene            complement(882..2114)
                     /locus_tag="AC16A0_05825"
     CDS             complement(882..2114)
                     /locus_tag="AC16A0_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3104883.1"
                     /translation="MTTRPASPEANALRHRPFVFYWLATGAAAFAVQIMSVAVGWQIY
                     DITRNPLHLGYVGLAQFLPPLLLVLVTGLAADRFSRRFIMATCLGVEALCALGLVVFT
                     LSHPTGVTPVFAILVVLGIARAFMNPAADALAPNLVPKAAIAHAISLNSMIWQIGNIV
                     GPVFGGLLYGISGVVAYGTALLLVGTAGILVLLIGRVPQQDHAQETSLSTLFAGFHFI
                     KKEKIVLGAISLDLFAVLLGGAVALMPIFARDILDVGPLGLGLLRAAPGIGAVVMALY
                     LARFGIKDHAGIVLFVFVAGFGFFTVVFGLSTWVPLSIAALLMMGACDMISVYVRETL
                     LQLWTPDDVRGRVNAVNRVFIGASNELGEFRAGVVAAWIGAVAAVVIGGAGTMGVAFF
                     WSRWFPELRTARRLDGKG"
     gene            complement(2178..2759)
                     /gene="tmpB"
                     /locus_tag="AC16A0_05830"
     CDS             complement(2178..2759)
                     /gene="tmpB"
                     /locus_tag="AC16A0_05830"
                     /EC_number="1.13.11.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-1-hydroxy-2-trimethylaminoethylphosphonate
                     oxygenase"
                     /protein_id="HIR3104884.1"
                     /translation="MTAPRLDHRNIVAFIADIFERRGAESYLGEAVTMSEHMLQGAVL
                     AEQEGAPEELVAAALLHDIGHYTSEFGTMSLGDERDNYHEESGAAVLAPFFPPVIAEC
                     VRLHVPAKRYLCATDASYFDRLSEASKHTLSLQGGPMSAAEVTAFEANPYHREAVRVR
                     IWDDEGKQPGMATPHFRHYMPMLQRVVDRHAGN"
     gene            complement(2756..>3374)
                     /locus_tag="AC16A0_05835"
     CDS             complement(2756..>3374)
                     /locus_tag="AC16A0_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR3104885.1"
                     /translation="GRLFDVRAEVNPNNLAYTNLGLQAHTDNPYRDPVPTLQILACLE
                     NTVEGGDSIVVDGFKVAERLQSENPEGFELLSSHSARFAYEGTRGVRLRAKRPMIELG
                     PDGELIAIRFNNRSAAAFRDIPYEVMEAYYAAYRRFAELVEDPAMEVTFKLKPGELFI
                     VDNTRVLHARKSFSGSGKRWLQGCYADKDGLLSTLAAIEEEGSAE"
BASE COUNT          678 a         1056 c         1064 g          576 t
ORIGIN      
        1 tgccggaatc gaaaagcaga atgcgcgcct ggctcatccc ttcggctccc tgcgccgttc
       61 atgtgcacgg gtcagcgaag cgacgatgcc gcgcaggctg cggacctcct gctcggtaag
      121 ttcggcccgg gcgaaaagat tgcgaatgtt gcgcagcatg ccaggcttct tgtccccagt
      181 cttgaagaac cctgccgtat ccagctcccg ctcgagatgg tcaagaaagc catagaactc
      241 ttccttcgtc gccgggcggg tatcgggcgt ctgcaggccg ggtccttcga gagccggcag
      301 ttctggcgtc tgctggccca gggtctctgc ctgttccttg taccattcat agcccaccag
      361 cagcacggcc tgggcgatgt tgatcgaggc aaaagccgga ttcaccggcg ccgtgacgat
      421 gacgtcagca agggacacct cgtcgttgtt gagaccccag cgctcgcggc cgaaaagcac
      481 cgcaaccttc tcgccgcgcg caatgcgtcg gcgcatgtca tgccctgcct cttccggcgt
      541 gatcacctcc ttgatcatgc cgcgcggccg cgcggtggtg gcatagacat aattcatgtc
      601 cgccagcgcc gaaggcagat cgggatggac ggtcgcggcc tcgatgatcc agttggcacc
      661 tgaggatgcc gtaagcgcct tctcgttcgg ccagccgtcc cgcgggtcga cgagacggag
      721 accgtgcagc ccgaaattgg ccattgcgcg ggctgccgtg ccgatgttct cgcccagctg
      781 cggattgacc agcaccgtca ccggcgcggg gccgagaacc tgattccgtg tcttgtcggt
      841 acctgccatg gccgggaaat agcggagttg cgcggtgccc gctagccttt gccgtccagc
      901 cggcgcgccg tccgcaattc cgggaaccag cgcgaccaga agaaggcgac ccccattgtg
      961 cctgccccgc cgatcacgac cgcggccacg gcgccgatcc aggccgccac cacgccggcg
     1021 cggaactcgc cgagttcgtt ggaagcgccg atgaacacgc ggttgaccgc attgacccgc
     1081 ccgcgcacat cgtcaggggt ccagagctgc aggagtgtct cgcgcacata gacgctgatc
     1141 atgtcgcagg cgcccatcat caacagcgcc gcgatcgaca atggcaccca tgtcgagaga
     1201 ccgaagacaa cggtgaagaa cccgaaacct gccacaaaga caaagaggac gataccggca
     1261 tgatccttga tgccgaaccg cgcgaggtag agcgccatca cgacagcgcc gatccccggc
     1321 gcggcacgca gcaggccaag gccaagcggg ccgacatcaa ggatgtctcg tgcaaagatc
     1381 ggcatcagcg ccactgcgcc gcccagcagc acggcaaaga gatcaagcga gatggcacca
     1441 agaacgattt tctccttctt gatgaagtga aacccggcaa acaatgtcga cagggaggtc
     1501 tcctgcgcgt gatcctgttg aggaacgcgc ccgatgagga gcacgagaat tcccgccgtg
     1561 ccgacaagca gcaaggcggt gccataggca accacacccg agatgccata gagaaggcct
     1621 ccgaagaccg gcccgacaat gttgccgatc tgccagatca tcgagttgag cgagatagca
     1681 tgggcaatgg ccgccttggg aacaaggttg ggcgccagcg catcggcggc cggattcatg
     1741 aaggcgcggg cgatgccaag caccaccaga atggcgaaga cgggggtcac gcccgtgggg
     1801 tgagacaggg tgaagaccac caggcccagg gcgcagagcg cctcgacacc caggcaggtg
     1861 gccatgatga agcgccggct gaatcggtcg gccgcaagac ccgtgacgag aaccagcagg
     1921 agcggtggaa ggaactgggc cagtccgaca tagccgaggt gcagcgggtt gcgcgtgatg
     1981 tcatagatct gccaacccac cgccaccgac atgatctgga ctgcaaaggc ggcagcacct
     2041 gtcgccagcc agtaaaagac gaagggccga tgccgcaacg cattggcttc gggcgaagca
     2101 ggtctggttg tcatgttccg gacctgccac tgggcgccgg tgaagtcaaa gcctgaccgg
     2161 catcatgcgg cacgtggtca gttgccggcg tggcggtcaa ccacgcgctg gagcattggc
     2221 atgtagtggc gaaaatgcgg agtagccatg ccgggttgct tgccctcatc gtcccagatc
     2281 ctcacccgca ccgcctcgcg atggtatggg ttcgcctcga aagccgtgac ttctgcggcg
     2341 ctcatcggac cgccttgcag agaaagcgta tgcttggagg cttcggaaag gcggtcaaaa
     2401 tagctggcat ccgtcgcgca gaggtagcgc ttcgccggca catgcaggcg cacgcattcg
     2461 gcgatcaccg gcggaaagaa tggcgccagc acggcggcgc cggattcctc atggtaattg
     2521 tcgcgttcgt caccgaggga catggtgccg aattccgacg tgtagtggcc gatgtcgtgc
     2581 agcagggctg ccgcaacgag ttcctccggc gcgccctcct gctcggccag cacggcaccc
     2641 tgcagcatgt gctccgacat tgtcaccgct tcgccaaggt aggactccgc cccgcgccgc
     2701 tcgaaaatgt ccgcgatgaa cgccacgatg ttccgatgat caagtcgcgg tgccgtcatt
     2761 ctgcagatcc ttcttcttcg atcgccgcga gtgtcgaaag cagcccatcc ttgtctgcat
     2821 agcagccctg gagccaacgc ttgccgctgc ccgagaaact cttgcgggcg tgcagcaccc
     2881 tggtgttgtc gacgatgaag agttcaccgg gcttcagttt gaatgtcact tccatcgcgg
     2941 gatcttcgac cagttccgca aagcggcgat aggcagcata gtaagcctcc atgacctcgt
     3001 aggggatatc gcggaaggcg gcagcagacc ggttgttgaa gcggatcgca atgagttcgc
     3061 catcggggcc aagttcgatc atgggacgct tcgcccttaa ccggacacct ctcgtgcctt
     3121 cgtaggcaaa gcgcgcgctg tggctgctga ggagttcaaa gccctccggg ttttcggact
     3181 gcaggcgttc ggccaccttg aagccgtcga caacgatgga gtctcctccc tcgaccgtgt
     3241 tctcgaggca ggccaggatc tgcaacgtgg gaaccggatc gcgataggga ttgtcggtat
     3301 gagcctgcag ccccaggttg gtgtaggcga ggttattggg gttcacttcc gcgcgcacgt
     3361 cgaaaaggcg gcca
//
LOCUS       DBNGVR010000273         2625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1082697, whole genome shotgun sequence.
ACCESSION   DBNGVR010000273 DBNGVR010000000
VERSION     DBNGVR010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1082697"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="AC16A0_05840"
     CDS             <1..657
                     /locus_tag="AC16A0_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR3104886.1"
                     /translation="QIAGVEICTEANDVHLTTAQGQCIRFPVNDVRVFKGRDSTGVRG
                     IRLEDGDRVISMSILRHVEATADERAAFLKMSRAVAGEETEAGVSDEETAADATLSQE
                     RYAELGAAEQVILTVSSNGYGKRTSSYEYRITGRGGKGIVAMAVNDRNGPLVASFPVQ
                     DEDEIMLVTDGGQLIRCPVHDIRKAGRSTQGVIVIDVADDEHVVSVEHISEDEAEGEA
                     "
     gene            686..1297
                     /locus_tag="AC16A0_05845"
     CDS             686..1297
                     /locus_tag="AC16A0_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3104887.1"
                     /translation="MTLETYVAFCLAAAALAFVPGPTVTVIIANSLRHGTSAGLMNVL
                     GTQAGFLIWLAIAALGLGAAIEVMGVWFDVLRYAGAAYLVWLAIKLFRSNGDLAVAVD
                     RARPNGSFFLQGFIVIISNPKMLVLFGAMIPPFITPGGDPMRQILLLGGTFMAIALAG
                     DTAYALLAGRAGAWLSRSRIRSLEIASGCFLMAGGVWMALRGR"
     gene            complement(1301..1690)
                     /locus_tag="AC16A0_05850"
     CDS             complement(1301..1690)
                     /locus_tag="AC16A0_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3104888.1"
                     /translation="MSLGTNDYERAKAFYGRVLKVIGCKLIMEHPGAAAFGKTYPEFW
                     INSPFDSKPATAGNGTHVGFAAQSKAEVDLFFKEALAAGATADGDPGHRHDYGEPYYG
                     CFVRDPDGHKIEATYWDMELARKLGMA"
     gene            1821..2321
                     /gene="coaD"
                     /locus_tag="AC16A0_05855"
     CDS             1821..2321
                     /gene="coaD"
                     /locus_tag="AC16A0_05855"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3104889.1"
                     /translation="MARIGFYPGSFDPVTFGHLDIIARSARLVDRLVLGVGVHHGKQA
                     LIAAGQRIELLDQVTRPIAEHTGVKISIETFDGLAVDAARRAHASLIIRGLRDASDFD
                     YEVQMGQMNGALAPDIETVFLAASPATRMIASSLVKQIARMGGDVTLFIPKEAVAVVK
                     AALKVT"
     gene            complement(2308..>2625)
                     /locus_tag="AC16A0_05860"
     CDS             complement(2308..>2625)
                     /locus_tag="AC16A0_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3104890.1"
                     /translation="RPPFNNTGAWMACGTLEIHLTVNPQVHFRPAPLVDTAEIHFAAR
                     VADFKATVRHLESLGYSGDKPDGDPKRLVFRLEGPAPYLQLYLVDPDNHVIEINDAAL
                     KSP"
BASE COUNT          421 a          804 c          878 g          522 t
ORIGIN      
        1 cagatcgcag gcgttgaaat ctgtaccgaa gcgaatgacg tgcacctgac caccgcgcag
       61 gggcaatgca tccgcttccc ggtgaacgat gtccgcgtgt tcaaggggcg cgattccacc
      121 ggcgtccgcg gcatcaggct tgaagacggc gacagggtca tctccatgtc gatcctgcgc
      181 cacgtcgaag ccacggcgga tgagcgtgcg gccttcctga agatgtcgcg tgccgtggcc
      241 ggcgaagaga cagaagctgg cgtttcggac gaggagacgg cagccgatgc aacgctgtcg
      301 caggagcgct atgccgagct gggcgcggcc gagcaggtga tcctgaccgt gtcgtccaac
      361 ggctatggca agcgcacgtc gtcctacgag taccgtatca ccggccgtgg cggcaaaggc
      421 atcgtcgcca tggcagtgaa tgaccgcaat ggtccgctgg tggccagctt cccggtgcag
      481 gacgaggacg aaatcatgct ggtcaccgac ggcggccagc tcatccgttg cccggtgcat
      541 gacatccgca aggccgggcg ttcgacgcag ggcgtcatcg tcatcgacgt ggccgatgac
      601 gagcatgtcg tgtcggttga gcacatcagc gaggacgaag ccgaaggcga ggcctgagcg
      661 ggacggggga agaggagggc gttgtatgac tcttgagact tacgttgcct tctgcctggc
      721 ggccgccgcg ctggccttcg ttccggggcc tacggtcacg gtcatcatcg ccaacagttt
      781 gcgccatggc acgtcggccg gactgatgaa tgttctgggc acccaggcgg gtttcctgat
      841 ctggctggcc attgccgccc tgggccttgg cgccgccatc gaggtcatgg gcgtctggtt
      901 cgacgtgctg cgctatgccg gtgccgccta tctcgtctgg ctcgccatca agctgttccg
      961 ctccaacggt gacctcgcgg tggcggtaga ccgggcccgg cccaatggca gcttctttct
     1021 gcagggcttc atcgtgatca tctccaatcc gaagatgctg gtgctcttcg gcgccatgat
     1081 cccgcccttc atcacgcccg gcggcgaccc gatgcggcag atcctgctgc tgggcggcac
     1141 cttcatggcg atcgccctgg cgggtgacac ggcttacgcc ctgctggcgg gccgcgccgg
     1201 ggcctggctg tcgcgctcgc gcatccgctc gctcgagatt gcgagcggat gcttcctcat
     1261 ggcgggtggc gtctggatgg ccctgcgcgg ccgctgagag tcaggccatg cccagctttc
     1321 gcgccagttc catgtcccag tatgtggcct cgatcttgtg gccgtcgggg tcgcgcacga
     1381 agcagccgta gtagggctcg ccatagtcat ggcgatggcc ggggtcgcca tctgccgtgg
     1441 caccggcggc aagcgcctcc ttgaagaaca ggtcgacctc ggccttcgac tgggccgcga
     1501 agcccacatg agtcccgttt cctgcggtgg ccggcttgct gtcgaaggga ctgttgatcc
     1561 agaattccgg ataggtcttt ccgaaggcgg ctgccccggg gtgctccatg atgagcttgc
     1621 agccaatgac cttcaggacc cggccgtaga aagcctttgc tctttcgtag tcattcgtcc
     1681 caagtgacac gtgtgaaatg cacgactggt tttcctctgc cattgtggcc tccttcgatg
     1741 cagatcctcg atacaatcct ttgctatcgt caccctgctg acaggttgcg gcagcatgat
     1801 gaagcgtcta gtcttgctcc atggcacgga ttggtttcta tcccggctcc ttcgaccccg
     1861 tcaccttcgg gcatctcgac attattgccc gctcggcgcg gctggtggac aggctggtgc
     1921 tgggtgtcgg cgtgcatcat ggcaagcagg ccctcattgc ggcgggccag cgcatcgagc
     1981 tgctggacca ggtgacgcgg cccatcgctg aacacacggg cgtgaagatc tcgatcgaga
     2041 ccttcgatgg ccttgcggtg gatgccgcgc ggcgcgccca tgccagtctc atcattcgcg
     2101 gcctgcgcga tgcgtcggac ttcgattacg aggtgcagat gggccagatg aacggcgccc
     2161 tggcgcccga tatcgaaacg gtcttcctgg ccgcttcgcc tgccacccgc atgatcgcct
     2221 cgtcgctggt caaacagatc gcccggatgg gtggcgacgt gaccctgttc atcccgaagg
     2281 aagccgtggc cgtggtgaaa gcggcgctca aggtgacttg agtgctgcgt cattgatctc
     2341 gatgacgtgg ttgtcggggt ctaccagata gagttgcagg tagggcgcgg gcccctccag
     2401 gcggaagacc agccgcttgg ggtcgccatc tggtttgtcg ccgctatagc ccagggattc
     2461 gaggtgccgg acggtggctt tgaagtccgc cacgcgggcg gcgaagtgga tctcggcggt
     2521 gtcgaccaat ggcgcggggc ggaagtgaac ctggggattg accgtcaggt ggatctcgag
     2581 agtcccgcag gccatccagg cgccggtatt gttgaaaggc ggccg
//
LOCUS       DBNGVR010000274         2687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1476442, whole genome shotgun sequence.
ACCESSION   DBNGVR010000274 DBNGVR010000000
VERSION     DBNGVR010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1476442"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="AC16A0_05865"
     CDS             <1..182
                     /locus_tag="AC16A0_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR3104891.1"
                     /translation="HDKIEVRNLDLDHPRLAGDDPTVVAIAANGEVADAPVPVFSRDD
                     VTALATFIERHMELA"
     gene            179..715
                     /locus_tag="AC16A0_05870"
     CDS             179..715
                     /locus_tag="AC16A0_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR3104892.1"
                     /translation="MTPEMVVNFWEAAGPSRWFTRDAAFDGALAVRFGEALAQARAGA
                     FDHWDETAEGALGLVILLDQVSRNVHRGSPLAFAADAKALALARTWIGMGYHQKLVAP
                     RAIWLLMPFEHAEDLDAQQRCVALFRTMGLSEMAHWAQVHLDIIARFGRFPHRNAVLG
                     RASTPAEIAFLKAGGFAG"
     gene            817..>2687
                     /locus_tag="AC16A0_05875"
     CDS             817..>2687
                     /locus_tag="AC16A0_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3104893.1"
                     /translation="MKTQARVVVIGGGVVGVGTLYHLAKKGWSDVVLIERKELTSGST
                     WHAAGLLPLFNLSYSVGQIHKYSVKLYGELEAETGQQVGFSKVSNIRLARTRDRWDEF
                     MYYAGVAETIGVKVNVLTPAQVKAIWPLCETDGLVGAIQHPDDGYIQPADLTQAMAKG
                     ARSRGAEIYRNTTVTAIEQKPNGEWLVKTDKGDITCEHVVSASGNFARKTGAMVGLDI
                     PVIPVEHQYIVTEPHPLIRERQAKGLPEMGVLREADSSWYMREENGGLLLGPYEHGAP
                     CCYMDGPSDQSEYELFQEDLDRLAPHIETAIARVPAFGEVGIKKVYNGAIAYTPDGSP
                     IIGPAWDRRNFWLNEGHSFGVTAAGGAGWQLAEWIVEGEPSIDMMGVDPRRFGPYATR
                     GYLKVKNEEAYANVFTPHYPDEERSAARPLKRTPCYDRMKDLGAVFGSVYGWERPGWF
                     APKDYALTKDELASPDVLTNHNYAPPTEDGRIVEKWSFRRSNYFKFVGEECRNVMNNL
                     ALQDMSAFAKMEVSGPGARDWLDSILANRIPKKMGRVALCHLLTPRGGVRAEFTVYEW
                     APGRFYLVSAGAYERHDHDVLRKLCPTDGSVRLNAITTRLGVLVLAGPNSRKVLQKLT
                     "
BASE COUNT          454 a          903 c          860 g          470 t
ORIGIN      
        1 gccacgacaa gatcgaagtg cgcaatctgg acctcgacca cccccggctt gcgggcgacg
       61 atccgacagt ggttgccatt gccgccaacg gcgaggtcgc cgatgcgccg gtgccggtct
      121 tcagccgcga cgatgtcacc gcgctggcaa ctttcatcga gcgccacatg gagctcgcat
      181 gacgcccgaa atggtcgtga acttctggga ggcggccggc ccttcgcgct ggtttaccag
      241 ggatgccgcc ttcgacggcg ccctggccgt gcgcttcggt gaagccctcg cccaggcgcg
      301 cgcaggggcc ttcgatcact gggatgaaac ggcggagggt gcactgggcc tcgtcatcct
      361 gctcgaccag gtgtcgcgca acgtccaccg tggcagtccg ctggcctttg ccgccgatgc
      421 caaggccctc gcgctggcca ggacctggat cggcatgggc tatcatcaga agctggtggc
      481 gccccgggcc atctggctgc tgatgccctt cgagcatgcc gaggacctcg acgcccagca
      541 gcgctgcgtg gccctgttcc gcaccatggg tctgtcggag atggcccatt gggcccaggt
      601 ccatctcgac atcatcgctc gcttcgggcg ctttccgcac cgcaatgcgg ttctggggcg
      661 ggcctccacg ccggccgaga tcgccttcct gaaggccggc ggattcgccg ggtgagtgtc
      721 gtaatccgcc ctgtcccggt cgttccgcct cacgcctgtt gaattcccct cactattgtg
      781 ccctccagtc gaggttccgg ttcggagggt tctcccatga agacacaggc gcgggtggtg
      841 gtgatcggcg gaggcgtggt cggtgtcggc accctctatc atctggccaa gaagggctgg
      901 agcgacgtcg tcctgatcga gcgcaaggag ctgacctccg gctccacctg gcatgccgca
      961 gggctgctgc cgctgttcaa cctctcctat tcggtgggcc agatccacaa gtattcggtg
     1021 aagctctatg gcgagctcga ggccgagacc ggccagcagg tggggttcag caaggtgtcc
     1081 aacatccgcc tcgcccgcac cagggaccgc tgggacgagt tcatgtatta cgccggcgtg
     1141 gccgagacca tcggggtcaa ggtcaatgtg ctgacgccgg cgcaggtcaa ggcgatctgg
     1201 ccgctctgcg agaccgacgg gctggtcggg gccatccagc atcccgacga cggctacatt
     1261 cagccggccg acctgaccca ggccatggcc aagggtgcgc gcagccgcgg cgccgagatc
     1321 taccgcaata ccaccgtcac cgcgatcgag cagaagccca atggcgagtg gctggtgaag
     1381 accgacaagg gcgacatcac ctgcgagcat gtcgtctcgg cctccggcaa cttcgcgcgc
     1441 aagaccggcg ccatggtcgg cctcgacatt ccggtaattc cggtcgagca ccagtacatc
     1501 gtcaccgagc cgcatccgct tatcagagag cgccaggcca agggcctgcc cgaaatgggc
     1561 gtgctgcgcg aggccgattc ctcctggtac atgcgcgagg agaacggcgg gctcctgctc
     1621 ggaccctacg agcatggcgc tccttgctgc tacatggatg ggccctccga ccaaagcgaa
     1681 tacgagcttt tccaggaaga cctcgaccgg cttgcgccac acatcgagac cgccatcgcg
     1741 cgtgtaccgg catttggcga agtcggcatc aagaaggtct ataacggcgc catcgcctat
     1801 acgcccgatg gctcgccgat catcggcccg gcctgggacc gccggaactt ctggctcaac
     1861 gagggccata gcttcggcgt caccgcggcg ggcggtgccg gctggcagct ggcggaatgg
     1921 atcgtcgagg gcgagccctc catcgacatg atgggcgtgg atccgcgccg cttcggcccc
     1981 tatgccacgc gcggctatct gaaggtgaag aacgaggagg cctatgccaa tgtcttcacc
     2041 ccgcattatc cggacgagga gcgctccgcc gcgcgtccgc tgaagcgcac gccctgttat
     2101 gaccgcatga aggacctggg cgccgtcttc ggttcggtct atggctggga gcggcccggc
     2161 tggttcgcac cgaaggacta tgcgctgacc aaggacgagc tggccagccc cgacgtgctc
     2221 accaatcaca attatgctcc gcccacggag gatggacgca tcgtcgagaa gtggtccttc
     2281 cgccgctcca actatttcaa gttcgtcggc gaggaatgcc gcaacgtgat gaacaacttg
     2341 gcgctgcagg acatgtcggc cttcgccaag atggaggttt cggggcctgg cgcgcgcgac
     2401 tggctcgact cgatcctggc caaccgcatc ccgaagaaga tgggccgcgt ggcgctctgc
     2461 cacctgctca caccgcgcgg cggcgttcgc gccgaattca ctgtctatga atgggcgccg
     2521 ggccgcttct atctcgtgtc cgccggcgcc tatgagcgcc acgaccacga cgtgctgcgc
     2581 aagctctgcc ccaccgacgg ctcggtgcgc ctcaacgcca tcaccacccg gcttggtgtc
     2641 ctggtgctgg ccggccccaa cagccgcaag gttttgcaga agcttac
//
LOCUS       DBNGVR010000275         2257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1869564, whole genome shotgun sequence.
ACCESSION   DBNGVR010000275 DBNGVR010000000
VERSION     DBNGVR010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1869564"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16A0_05880"
     CDS             complement(<1..461)
                     /locus_tag="AC16A0_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104894.1"
                     /translation="MTSICSFFRIAIVCAIGLAAQSALAEADSFKVRFKPGAESARYT
                     GSIQGYDDDTYIFDARRGQEMTVSLRTSNPQAYFNILPPGSDVAIYNGSIEGNRYTGV
                     LPRSGKYRIVVYMMRAAARRDEVADYRLSVAIPAEEQDTPSGDFADGNAGGP"
     gene            complement(506..1489)
                     /locus_tag="AC16A0_05885"
     CDS             complement(506..1489)
                     /locus_tag="AC16A0_05885"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3104895.1"
                     /translation="MNITRRRFHEILLGSAGFGALTGQASAQLVTKYAEDLRNGEFNW
                     FPERSPSGPLLIIVSIPDQKVHVYRNGIRIAASTCSTGKPGHSTPTGVFKILQKDKHH
                     RSSTYSNAPMPNMNRLTWSGIALHAGNLPGYPASHGCVRLPMEFSERLFGLTRTGMTV
                     VIADNKSYPTEVAHPGLVLGDYARHEFGGVDASVKRSQYSEGRAAPQKSTSLVVSGKD
                     RSLSVFEDDQLVAQGKVTIRDAHRPIGEHVHTLTGTDAKRQQVRWSSASHGKQDKADL
                     AADLGRIQAEPKMREEVRKRMHHGTTVVTTNESSTPSHRSARDFVVIDGIY"
     gene            complement(1500..>2257)
                     /locus_tag="AC16A0_05890"
     CDS             complement(1500..>2257)
                     /locus_tag="AC16A0_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3104896.1"
                     /translation="SGRGLAVTPSGQRLAERLSATFANLDASLEDIVGGNAQVFRVAV
                     CSSFGPGWLIERLADFYDAYPEIDLQLKLYAQDPLISGEVADAFVTANPVKPDYVSVR
                     LKDEMLLAVHKPSAKRGKRHRLITTWLDKGHIGQEWADYCKAAGLKLSEFQDGAFRQC
                     THYLFALEMAKSGQGVALVPDFLAARDIAAGTVTAFSRRLVPSGRAYRLCYRKSRAHE
                     PQLGALRDWLLSGMAEKPGRRRRLVEIQNVAKP"
BASE COUNT          389 a          717 c          711 g          440 t
ORIGIN      
        1 ggacctccgg cattgccatc ggcgaagtca ccggacggcg tgtcctgctc ttcggccgga
       61 atggcgaccg atagccggta gtccgccact tcgtcgcggc gggcggcagc gcgcatcatg
      121 tagaccacga tgcgatactt gccggatcgc ggcagcacgc cggtgtagcg gttgccctcg
      181 atcgatccat tgtagatcgc cacatcggat cctggcggaa gaatgttgaa gtaggcctga
      241 ggattgctgg tccgcaggct cacggtcatc tcctggccgc ggcgtgcgtc gaagatgtag
      301 gtgtcgtcgt catagccctg gatcgaaccc gtataccgcg cactttccgc accgggcttg
      361 aagcgaacct tgaagctgtc ggcctcggcc agcgccgact gggctgcgag gccaatggca
      421 cacacgattg caatcctgaa gaagctgcag atcgacgtca tcccgtttct ctccctgtcc
      481 ttgcgacggc gccgccttga ctgtctcagt aaatgccgtc gatgacgacg aagtcacggg
      541 cgctgcgatg cgacggtgtg gacgactcgt tcgtcgtcac caccgtcgtg ccgtggtgca
      601 tgcgcttcct gacttcctcc cgcatcttcg gttccgcctg gattcggccc agatcggccg
      661 cgagatccgc cttgtcctgc tttccatggc tggccgaaga ccatctgacc tgctggcgtt
      721 tcgcatctgt gccagtcagc gtgtgcacat gctcgccgat cggcctgtgc gcgtccctga
      781 tggtcacctt gccttgcgcc accagctggt catcctcgaa gaccgacagg ctcctgtcct
      841 tgccgctcac caccaggctg gtggacttct gcggggccgc ccgaccctcg ctgtactggc
      901 ttctctttac cgaggcatcg acaccgccga attcatgccg cgcgtagtct cccagaacaa
      961 ggccgggatg ggccacctca gttggatagg acttgttgtc ggcgatgacc acggtcatgc
     1021 cggttcgggt gaggccgaag aggcgttccg agaactccat cggcaggcgc acgcatccat
     1081 gcgaggcagg atagccgggc aggttgccgg catgcagcgc aatgccggac caggtgagcc
     1141 ggttcatgtt cggcatgggc gcgttgctgt aggtcgagga acggtgatgc ttgtccttct
     1201 gcaggatctt gaacacgcct gtgggtgtcg aatggccggg ctttccggtc gaacaggtgg
     1261 aggccgcaat gcggatgccg ttgcgataga cgtgtacctt ctgatcgggg atcgagacaa
     1321 tgatcagcag cggcccgctg ggcgagcgct cgggaaacca gttgaattct ccgttccgca
     1381 ggtcctcggc atattttgta acaagctggg ccgaggcctg gccggtcagg gccccaaacc
     1441 ccgcggagcc aaggaggatc tcatgaaagc ggcggcgcgt gatgttcatg actttcccac
     1501 tacggctttg ccacattctg gatctccacg agtctgcgcc gccggcccgg tttctccgcc
     1561 atcccggaca acagccagtc acgcagggca ccaagctggg gctcatgcgc gcggctcttc
     1621 cggtagcaca gccggtaggc acgcccggat ggcaccagcc tccggctgaa ggccgtgacc
     1681 gtgcctgccg caatgtcgcg cgcggcgagg aagtccggca ccagcgccac gccctggcca
     1741 ctcttggcca tttcgagcgc gaagagatag tgcgtacact gacgaaacgc gccatcctgg
     1801 aattccgaaa gcttcagacc cgctgccttg cagtagtcgg cccattcctg gccgatgtgg
     1861 cccttgtcca gccaggtggt gatgagccgg tggcgcttgc cgcgtttcgc cgatggcttg
     1921 tgaaccgcaa ggagcatctc gtccttgagc ctgacggaca cataatccgg cttgaccggg
     1981 ttggccgtga cgaaggcatc ggccacttcg ccgctgatca ggggatcctg cgcatagagc
     2041 ttcagctgca ggtcgatctc gggataagcg tcgtagaaat ctgcgagccg ttcgatcagc
     2101 cagcccgggc cgaagctgct gcaaaccgcg acgcggaaga cttgcgcgtt gccgccgacg
     2161 atgtcctcaa gagatgcatc gaggttggca aacgtcgcgg acagccgttc ggcaagccgc
     2221 tgccccgaag gcgtcaccgc gagtccgcgc ccggagc
//
LOCUS       DBNGVR010000276         8210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1869709, whole genome shotgun sequence.
ACCESSION   DBNGVR010000276 DBNGVR010000000
VERSION     DBNGVR010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1869709"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC16A0_05895"
     CDS             <1..539
                     /locus_tag="AC16A0_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104897.1"
                     /translation="RRLIVAAARGMVVPVQAPRVARALRIVSSGTGAGRVTFQLFDPR
                     RVLWSDRRGYGAGARGRPRARFIDVAFDPRIPLFRPAPRVEADQAEEPDDHTINALPL
                     CRRLSAIRAALDDLPRQAQRYARWRARPRETRRPQRHDALRIGPPPGLKRRRTREVDE
                     ILSECHWLARHAAASDTS"
     gene            complement(617..1573)
                     /locus_tag="AC16A0_05900"
     CDS             complement(617..1573)
                     /locus_tag="AC16A0_05900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3104898.1"
                     /translation="MRTSRRHVLMGTAATAAAALLPGNAARAQTTQLGVAPADSHRYK
                     IGGYEVTAILDFTRPAEKPETIFGTNQKPEDVAALLEANFLPTNVTMNMFTPVVVNTG
                     KELVLFDTGLGAAAGGQLPQRLAAAGIAPDQINVVVITHCHPDHIGGVAADGKPTFPN
                     ARYVIGETEYGFWSAPERLSGPTENAAKLVAANVTPFKEKMTFIKDQDEVAAGIRAVA
                     APGHTPGHTAFHIESEGKRLLLGADFCNHYVLSLQRPDWEVRFDADKAAAAATRKKLL
                     DMIATDRIPFTSYHMPFPAVGYVEKTSDGGYRYVPATYQLTL"
     gene            1706..3136
                     /locus_tag="AC16A0_05905"
     CDS             1706..3136
                     /locus_tag="AC16A0_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR3104899.1"
                     /translation="MVKPSILWLRQDLRLADHAALTAAAAAGPVIPLYVLDDETPGNW
                     RMGAASRWWLHKSLESLGRRVPLVLLRGRADVVISELLGQSGATSLHFTRDYAPWSAG
                     LERRVKDACDAAGVACHRYGGYLLHEPEALRNGLGEVYKVYTPFSKACFAAGEPRSPR
                     PVPSFKTSDHALKGDELASWDLLPRRPNWARGFEACWEPGEEGARRRLAAFIANGLAD
                     YSDARDRPDRDVTSRLSAHLHFGEISPAQCWHAVRTAQSAANGRLDRAAEKFLKEILW
                     REFSYHLLHLRPDLPEQPFRPEFSAFPWQVNEADLVLWQQGRTGYPIVDAGMRELWSS
                     GIMHNRVRMIVASFLIKDLLIPWQAGERWFWDTLVDADIASNAASWQWVAGCGADAAP
                     YFRIFNPVLQGEKFDPVGAYVRKWVPELAQLPDPFVHRPWQAPTPVLQAAGVELGRNY
                     PHPLIDHGMARDRALAAFKAIKGAAE"
     gene            3133..3678
                     /locus_tag="AC16A0_05910"
     CDS             3133..3678
                     /locus_tag="AC16A0_05910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR3104900.1"
                     /translation="MNILGISGALRMASTNTGLLRALKEMAPATAAFEIATLHGIPLF
                     NEDDETATGKPQAVRDLDARIRAADGIVIACPEYNFSIPGVLKNATDWLSRGGSPLRW
                     KRVGIVGAGGGQFVGTARAQYHFRQNLQALQAITMPRPEVFVNHNEQKFDAAGNLTDD
                     ETRHHLAKWFESFLEWVEKKP"
     gene            complement(3688..4356)
                     /gene="rpe"
                     /locus_tag="AC16A0_05915"
     CDS             complement(3688..4356)
                     /gene="rpe"
                     /locus_tag="AC16A0_05915"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3104901.1"
                     /translation="MARPLIIAPSILAADFARLGDEVRAIDAAGADWIHIDVMDGHFV
                     PNISFGTPIMQSIRDVTKKPFDVHLMIAPVDPYLESFARAGADIITVHAEAGPHLDRT
                     LQAIRTMGKKAGVSLNPATPASVLRHVLDRLDLILVMSVNPGFGGQAFIPAMLDKISE
                     LREMTRGRNIDIEVDGGIGVENAGEILRAGANALVAGSSIFKTQDYAAAIKSIRMAAT
                     GVAI"
     gene            complement(4361..5371)
                     /gene="gap"
                     /locus_tag="AC16A0_05920"
     CDS             complement(4361..5371)
                     /gene="gap"
                     /locus_tag="AC16A0_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3104902.1"
                     /translation="MPKVRVAINGFGRIGRNIVRAIYESGRKDIDVVAVNDLGPVETN
                     AHLLRYDSVHGRFPHVVKVDGDSINIGSDKFKVTAIKDPAQLPWKDLGVDIALECTGI
                     FTSRDKASAHLTAGAKRVIVSAPADGADLTVVYGVNHDKLTKDHIVISNASCTTNCLA
                     PVAKVLNDTVGIDHGFMTTIHAYTGDQPTLDTMHKDLYRARAAAMSMIPTSTGAAKAV
                     GLVLPELKGKLDGVAIRVPTPNVSVVDFKFVAKRKTTPEEINAAIKAAADGPLRGILG
                     YTDEPNVSIDFNHNATSSTFHMDQTKVMEGTLVRVLSWYDNEWGFSNRMSDTAVAMGK
                     LL"
     gene            complement(5460..6113)
                     /locus_tag="AC16A0_05925"
     CDS             complement(5460..6113)
                     /locus_tag="AC16A0_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR3104903.1"
                     /translation="MPSSVADSGLGRKASKEVRKQQLIEATIRVLAVKGFAALTVADV
                     AKAAGLSTGIIIFHFVSKDELLASVLRFLAEEYYNNWKQAVAAAEDNPAAKLSALVLA
                     DFNPDVYTPDKLAAWIAFWGESQGRPIYEELCAGFDAERLVAGERFCSSLIESGGYDL
                     DPDITMKALDAMGDGLWLAVAQSGSGLTGRLTPQECNQIMHASLAAFFPRHFAQPGR"
     gene            complement(6113..6901)
                     /locus_tag="AC16A0_05930"
     CDS             complement(6113..6901)
                     /locus_tag="AC16A0_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3104904.1"
                     /translation="MAEADSGELYGNKLIRMLEIVWGDGYLSPGGPDEVGRVLAGLEL
                     TGKSVLDIGSGAGGIDIQLVREHGAGHVTGIDVEDTVIATARGKVVAAGLEDRIGFAK
                     VAPGPFPFPPACFDVVFSKDSIVHIPDKHALAREVFRVLRPGGWFAASDWLIGHDGEP
                     SPQMKEYIKAEGLDFGMASPARYRAALQDAGFAEIAITSRNAWYRDQARREVESMRGP
                     LYAQAAAELGRDFVDHNIEIWNRMLKVLDSGEHCPTHIRARRPV"
     gene            7045..>8210
                     /locus_tag="AC16A0_05935"
     CDS             7045..>8210
                     /locus_tag="AC16A0_05935"
                     /inference="COORDINATES: protein motif:HMM:NF023330.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HIR3104905.1"
                     /translation="MRDHELRNVLNDELHGRPGLPVTAPARVTHLAFTIAEGDPDPLI
                     NVKQLCDALGVKPPVDGAAHHSVEIKGGQIKYERHGEFYRISVIAEGGGDGEAIALLP
                     LGWLDGLPGRRLVAIHTRILSASDPTPDTDALVRIFGHDDIAGSKVSSGQATVWTDFR
                     IGPDGYTRMLVQDHGLSPLRLGRVVRRIHEIETYRMMALLALPLARRIQAELVGLERA
                     VSKVTADMLSSRESHEDAELLGQLSGIARDVEEISSRSSYRFAAARAYAALVDKRIEE
                     LGEEKVMSFQRIGVFLDRRFGPAMATCRAVADRITNLAERSERASNLLRTRVDIALEG
                     QNQKLLASVDKRGQQQLRLQQTVEGLSVVAISYYMIGIVTKLIDGLGSYVPALDT"
BASE COUNT         1420 a         2586 c         2699 g         1505 t
ORIGIN      
        1 tgcggcggct gatcgtggcc gcggcccgcg ggatggtggt gccagttcag gcgccgcgtg
       61 tggcgcgcgc gctgcgcatc gtctcatctg gcacgggagc gggccgcgtc acgtttcagc
      121 tcttcgatcc gcgccgggtt ctgtggagtg accgtcgcgg atacggcgca ggcgccaggg
      181 gcaggccgcg cgcccgcttc atcgacgtcg cattcgatcc ccgcattccc ttgttccgtc
      241 cggcacctcg ggtcgaagcc gatcaagccg aggagccgga cgatcacacc atcaacgccc
      301 tgccgctgtg ccggcggctt tccgccatcc gtgccgccct cgatgacctg ccccgccagg
      361 cgcagcgcta tgcccgctgg cgggccaggc cacgcgagac ccggagaccc cagcgccatg
      421 acgcgctgcg catcggcccg ccgccgggtc tcaaacggcg caggacccgc gaggtggacg
      481 agatcctctc cgaatgccac tggctcgccc gccatgcggc ggccagcgac acgtcatgac
      541 gacgaaatct ccccccaggt acgtgccggt ccgccacgcc ccaactcgca atcgatttag
      601 acgcagtcgg ttttgcctag agcgtcagct gataggtggc cggcacatag cgataaccgc
      661 catcggatgt cttctccaca tagcccacgg ccgggaatgg catgtgatag ctggtgaagg
      721 gaattcgatc agtggcaatc atgtcgagca gctttttgcg cgtcgcggcc gccgcggcct
      781 tgtcggcatc gaagcgaact tcccagtccg gccgctgcag cgacaggacg tagtgattgc
      841 agaagtcggc acccagcagc aggcgcttgc cttcgctctc gatgtggaag gccgtatggc
      901 ccggggtgtg gcccggcgcc gccacggcgc gaatgccggc ggcgacctcg tcctggtcct
      961 tgatgaaggt catcttttcc ttgaagggcg tgacatttgc cgcgaccagc ttcgcggcat
     1021 tctcggtcgg acccgacagg cgctcgggcg ccgaccagaa accatactcc gtctcgccga
     1081 tgacgtagcg ggcattgggg aaggtgggct tgccgtccgc tgccacgccg ccaatgtgat
     1141 cggggtggca atgggtgatc accaccacgt tgatctggtc gggcgcgatg ccggcagccg
     1201 cgaggcgctg cggcagctgg ccgcccgctg cggcacccag cccggtgtcg aagagcacca
     1261 gttccttgcc ggtgttgacg acgaccggcg tgaacatgtt catggtgacg ttggtgggaa
     1321 ggaagttcgc ctccaggagg gcagccacgt cttccggctt ctgattggtg ccgaagatgg
     1381 tttccggctt ctccgccggg cgcgtgaagt cgaggatggc ggtcacctca tagccgccga
     1441 tcttgtagcg gtggctgtcg gctggagcga ccccaagctg ggtcgtctgg gcgcgggcgg
     1501 cgtttccggg caacagggcg gccgcagccg tcgcggcagt tcccatcagc acatggcggc
     1561 gagacgttct catcggagtc ctcctgaaat tggtaacggc caatgatacg cttgaatcgt
     1621 ggcaggctat cagtttttgc cccggtctca cgggcttgtg atcgggtgat gatccgcggc
     1681 agggtcgctc cgtaaaaggg gtaccatggt gaaaccgtcc attctctggc ttcggcagga
     1741 tctgcgcctg gccgatcacg cagccctcac ggctgccgca gcggctgggc cggtgatccc
     1801 tctctatgtt ctcgatgacg agacacccgg gaactggcgc atgggggctg cgtcgcgctg
     1861 gtggcttcac aagagccttg aaagcctggg ccggcgagtt ccgctcgttc tgctgcgggg
     1921 gcgcgctgat gttgtcatct cggagcttct cggccaatca ggggccacga gcctgcactt
     1981 tacgcgcgac tacgcgccct ggtccgcagg gctcgagcgt cgcgtcaagg atgcctgtga
     2041 tgcggcaggc gtcgcctgtc atcgctacgg cggctatctg ctgcatgagc cggaagcact
     2101 ccgcaatggc ctgggcgagg tctacaaggt ctacacgccc ttctcgaagg cctgcttcgc
     2161 ggccggtgaa ccgaggagtc cgcggccggt accgagtttc aagacaagcg accacgcgtt
     2221 gaaaggcgat gaactggcca gctgggactt gctgccaagg cgccccaact gggccagggg
     2281 gttcgaggct tgctgggagc caggtgaaga gggcgcccgc agaaggctcg cggccttcat
     2341 tgccaacggg ctcgccgact attccgatgc gcgcgatcgt ccggaccggg atgtgacctc
     2401 aagactttcc gcccacctgc atttcggcga gatcagtccg gcacagtgct ggcatgcggt
     2461 ccggacggcg caatctgccg ccaatggccg cctcgaccgg gcggccgaga agttcctgaa
     2521 ggaaattctc tggcgcgagt tctcctatca cctgctgcac ttgcggcccg atctgccgga
     2581 acagccgttc cggcccgagt tctccgcctt cccctggcag gtgaatgagg cggaccttgt
     2641 cctctggcag cagggtcgaa cgggttaccc catcgtcgac gcgggcatgc gtgaactctg
     2701 gtccagcggg atcatgcaca accgcgtccg catgatcgtg gcctccttcc tcatcaagga
     2761 tcttctcatt ccatggcagg caggcgagcg ctggttctgg gacacgctgg tcgacgccga
     2821 catcgccagc aacgctgcaa gctggcaatg ggttgcgggg tgcggcgccg atgccgcacc
     2881 ctatttccgg atcttcaatc ccgtgctgca gggcgagaag ttcgatcccg tgggcgccta
     2941 tgtgcgcaag tgggtgccgg agcttgccca gcttcccgat ccgttcgtgc accggccgtg
     3001 gcaggcgccc acgccggtcc tgcaggcggc cggggttgaa ctgggacgca actacccgca
     3061 tcctctcatc gatcatggca tggcgcgtga cagagcgctt gccgccttca aggccatcaa
     3121 gggagcggcg gaatgaacat actcggcatc agtggcgcgt tgcgaatggc atcgaccaat
     3181 accggtcttc ttcgggctct gaaggagatg gcgccggcca ccgcggcatt tgagattgcc
     3241 acgctccacg gcattccgct cttcaacgag gatgacgaaa cggcgacagg caagccgcaa
     3301 gccgtgcgcg accttgatgc ccgtatccgc gccgccgacg gcatcgtgat cgcttgcccg
     3361 gagtacaatt tctcgatacc gggcgtgctg aagaatgcga ccgactggct gtcgcgcggc
     3421 ggttcgccct tgcgctggaa gcgcgtcggc atcgtcggtg cggggggcgg ccaattcgtc
     3481 ggcacggctc gcgcgcagta tcattttcgc cagaacctgc aggccctgca ggccattacc
     3541 atgccacgcc ccgaggtctt cgtgaatcac aacgagcaga agttcgatgc ggcaggcaac
     3601 ctgaccgacg acgagacccg ccaccacctg gcgaagtggt tcgagtcctt cctggaatgg
     3661 gtcgagaaga agccgtgatg gccaggatca gattgcgacg ccggtcgcgg ccatgcggat
     3721 tgacttgatc gccgcggcat aatcttgggt cttgaagatg ctcgatccgg cgaccagggc
     3781 attggcgcct gcgcggagga tctcgcctgc attttccaca ccgatgccgc cgtcgacctc
     3841 gatgtcgatg ttccggccgc gtgtcatctc gcgaagttcc gagatcttgt cgagcatcgc
     3901 cgggataaag gcctgaccgc cgaaacccgg attgaccgac atgaccagga tgaggtcgag
     3961 ccgatccagt acatgcctga gaaccgaagc gggcgtcgcc ggattgagcg agacgccggc
     4021 tttcttgccc atggtcctga tcgcctgcag ggtgcgatcg agatgaggac cggcttcggc
     4081 atggacggtg atgatgtcgg ccccggccct ggcgaagctc tcgagatagg gatcgacggg
     4141 cgcgatcatg aggtgaacgt cgaagggctt ctttgtgacg tcgcggatgg attgcatgat
     4201 cggcgtaccg aagctgatgt tgggcacgaa gtgcccatcc atcacatcga tatgaatcca
     4261 gtcagccccg gccgcatcga tggcccgcac ctcatcaccc agccgcgcga agtcggcggc
     4321 gaggatcgag ggcgcgatga tcagcggccg ggccatggtg tcagagcagc ttgcccatgg
     4381 ccacggccgt gtcgctcatg cggtttgaga agccccactc gttgtcatac cacgagagga
     4441 cgcgcaccag cgtaccttcc atgaccttgg tctggtccat gtggaacgtc gacgaagtgg
     4501 cgttgtggtt gaagtcgatc gagacattgg gttcgtcggt atagccgagg atgccccgga
     4561 gcgggccgtc ggccgcagcc ttgatggcgg cgttgatttc ttccggcgtg gtcttgcgct
     4621 tggcgacgaa cttgaagtcc accaccgaga cgttcggggt gggcacccgg atggcgacgc
     4681 catcgagctt gcccttgagt tccggcagca cgaggcccac ggccttggcg gctcccgtcg
     4741 aggtcgggat catcgacatg gcggccgcgc gggcgcggta gagatccttg tgcatcgtat
     4801 ccagcgtcgg ctggtcgccg gtgtaggcgt ggatggtggt catgaagccg tgatcgatgc
     4861 cgacagtatc gttcagcacc ttggccaccg gcgcgagaca gttggtggtg caggaggcat
     4921 tcgagatcac gatgtgatcc ttggtcagct tgtcgtggtt cacgccgtag accaccgtca
     4981 ggtcggcgcc atccgcggga gccgagacga tcacgcgctt ggcgccggcc gtgaggtggg
     5041 ccgaggcctt gtccctggac gtgaagatgc ccgtgcattc cagcgcgatg tcgacgccga
     5101 gatccttcca ggggagttgg gccgggtcct tgatggcggt caccttgaac ttgtcggatc
     5161 cgatgttgat cgagtcgccg tcgaccttca ccacgtgcgg gaagcggccg tgaacggaat
     5221 cgtagcggag caggtgggca ttggtctcga ccgggcccag atcgttcacg gccacgacgt
     5281 cgatgtcctt gcggccggac tcgtaaatgg cgcggacgat gttgcggccg atgcggccga
     5341 agccgttgat ggcgacgcgg acttttggca tggagacctc cgttgcaaat gaccttggat
     5401 ttctgcgccc ttatagccaa tcttgggcag ggcggaaggg aaaatcagag gtgcgcccct
     5461 cagcggcctg gctgtgcgaa gtgtcgcggg aaaaaggcgg ccagcgaggc atgcatgatc
     5521 tggttgcatt cctgcggcgt cagtcggccg gtgagaccgc tgccggactg ggcgaccgcc
     5581 agccaaagtc catcgcccat ggcatccaat gccttcatgg tgatgtcggg atcgaggtca
     5641 tagcctccgg actctatcag ggagctgcaa aacctttccc ctgcgactag ccgctcggcg
     5701 tcgaagccgg cacagagttc ctcgtagatc ggccgtccct gcgactcgcc ccagaaggca
     5761 atccaggcag caagtttgtc gggtgtgtag acgtccggat tgaaatccgc cagcaccagt
     5821 gcggagagct tggcggcagg attgtcctcg gcagcagcga ccgcctgctt ccagttgttg
     5881 tagtattcct cggcaaggaa gcgcagtacc gaggcgagga gttcgtcctt gctgacgaag
     5941 tggaagatga tgatgccggt cgacagaccg gcggcctttg cgacgtcggc gaccgtaaga
     6001 gccgcaaagc ccttcacagc gaggacgcgg atcgttgcct cgatcaactg ctgcttgcgc
     6061 acctccttcg aggccttgcg cccaaggcca gagtcggcga ccgacgatgg catcagacgg
     6121 gtctcctggc gcggatatgg gtggggcaat gttcgccgct gtccagaacc ttcaacatgc
     6181 gattccagat ctcgatgttg tggtcaacga agtcgcggcc aagctcggcg gccgcctggg
     6241 cataaagcgg ccctcgcatg gattcgactt cccggcgggc ctgatcccga taccaggcat
     6301 tgcggctggt gatggcgatc tcggcaaatc ccgcgtcctg aagcgccgcc cggtagcgcg
     6361 ccggcgaggc catgccaaaa tccaggccct cagccttgat gtactccttc atttgtgggg
     6421 atggctcgcc atcgtgtccg atcagccaat cggatgcggc gaaccagcct ccaggccgca
     6481 ggacccggaa gacttcgcgg gccagcgcat gcttgtcggg aatgtggacg atggagtcct
     6541 tgctgaagac cacatcgaag caggccgggg ggaagggaaa tggaccgggc gccaccttgg
     6601 cgaagccgat gcggtcctcc agccccgcgg ccacgacctt gccgcgcgcc gttgcgatga
     6661 ccgtgtcctc gacatcgatg ccggtcacat gtcctgcacc atgctcccta accaactgga
     6721 tatcgatacc gcccgcaccg cttccgatgt ccagtaccga cttgcccgtg agttccaacc
     6781 cggccagcac ccggccgacc tcgtcgggtc cgccgggcga caggtacccg tcaccccata
     6841 cgatctcaag catgcgaatg agcttgttgc cgtaaagctc gccggaatct gcctctgcca
     6901 tcgggaatcg ccttgcccgt ccaaaactgg caagaccttt gtccagccgc gaggacttgg
     6961 caagaccaag ctttcggggc actgtcactt ggacacattg acccctcgcc gggctgccca
     7021 tatgggtgtg gcaggggaac attcatgcgc gatcacgaac ttcggaacgt cctgaacgac
     7081 gagttgcacg gcaggcccgg ccttccggtc acggcgccgg cgcgggtgac gcacctggcc
     7141 ttcaccatcg cggaaggcga cccggatccc ctcatcaacg tgaagcagct ctgcgacgcg
     7201 ctgggggtga agccgccggt cgacggggcg gcgcatcact cggtggaaat caagggcggc
     7261 cagatcaaat atgaacgcca tggcgaattc taccggatca gcgtgattgc cgaaggcggc
     7321 ggcgacgggg aggcgatcgc gctgttgccg ctcggatggc tggacggatt gcccggcagg
     7381 cggctggtgg ccatccacac gcgcatcctc tcggcttcgg atccgacgcc cgacaccgac
     7441 gcgctggttc gcattttcgg gcacgacgac attgccggct ccaaggtgag ttccggtcag
     7501 gccacggtct ggaccgactt ccgcatcggg ccggatggtt atacccggat gctggtgcag
     7561 gaccacgggc tctcgccatt gcggctgggc cgggttgtcc gccgcattca cgagatcgag
     7621 acttaccgca tgatggcatt gctggccctg ccgctggcgc gcaggatcca ggcggagctg
     7681 gtggggctcg aacgcgccgt ctccaaggtg acggccgaca tgctgtcctc gcgggaaagt
     7741 cacgaagacg ctgaacttct cggccagctc agcggcatcg cccgcgacgt ggaagagatc
     7801 tcgagccgtt cgagctatcg ctttgccgcc gcccgggcct atgcggcgct ggtcgacaag
     7861 cgcatcgaag aactgggcga ggagaaggtg atgagcttcc agcgcatcgg cgtctttctc
     7921 gaccgccgct tcggaccggc catggccacc tgccgggcgg tggccgaccg catcaccaat
     7981 ctggccgaac gcagcgaacg cgccagcaat ctgctgcgca cccgcgtcga cattgcgctc
     8041 gagggccaga accagaaact gctggcctcc gtcgacaagc gcggccagca gcaactccgc
     8101 ctgcagcaga ccgtcgaggg cctctcggtg gtggcgatca gctattacat gatcggcatc
     8161 gtcaccaagc tcatcgacgg gttgggcagc tatgttccgg cgctcgacac
//
LOCUS       DBNGVR010000277         7053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2065761, whole genome shotgun sequence.
ACCESSION   DBNGVR010000277 DBNGVR010000000
VERSION     DBNGVR010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2065761"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="AC16A0_05940"
     CDS             <1..1359
                     /locus_tag="AC16A0_05940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3104906.1"
                     /translation="RSLPATEILLEGGRGVTAAVQVTVVPWHGGPARRAVLRHADLPR
                     GEALAQKTGPAEADQAGAPPLQTAEIIQLPQRNGHAEADDELRAILDTASDGIITLDA
                     QSRIHSFSAGAEAIFGYRIAEVAGKSFAELFVPESRRVLRDYLAALQGPGLASVFNDG
                     REVQAVVKQGGTVPLFLTIGRLQAPKSQAAFCAVVRDITQWKKTESELREAKERAEAT
                     SRQKSEFLARISHELRTPLNAIMGFSEVMRLARFGEIENERYRGYVNDIHASGSHLLS
                     LINDLLDIAKVEAGKLELNFTAVNLGEVIDHAMKMLQEEATSARVVLRKAWPQGLPNV
                     VADLRSMRQIMLNLLSNAIKFTDPGGEVIVSGQLTEAGELKLRVKDTGIGMDADQLRH
                     ALEPFRRVLTEGREAQGTGLGLPLTKALAEANRAAFAISSEPRKGTLAEITFPTTRVL
                     AE"
     gene            1356..3053
                     /locus_tag="AC16A0_05945"
     CDS             1356..3053
                     /locus_tag="AC16A0_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104907.1"
                     /translation="MTEASLPRRRHGQHLAPGSRFLRVAALVIGGLLALPVITIIFLA
                     ATPAENIWPHLVATVLPGYIWQTALLLAGVGAITFVVGTAVAWLVTACSFPLRRVFQW
                     ASLMPMAMPGYIVAYTYVDLLSYAGPVQTWLRGLFGWKTPADYWFPEIRSMGGAIIVL
                     SMVLYPYVFLTARASFIRQPGMQMEVARALGQTAWGAFRRVALPLARPAIAVGVSLAL
                     MECVNDIGAAGFFGVRTLTLGIYTTWLSQGNLGGAAQIAAVMLVFVFALLWIERAARR
                     QRSFVMPTRRPRAADRVRLRGIRGWIAFAVCLIPIVIGFIVPALTLVNFALHHLDQAV
                     SSSFFKAASHSLVLATTAAAIAVCLGLILGYSNRMAPGGLTRKVIRLASMGYAIPGTV
                     LGIGVLIPLAAFDNAIDTLMRDRFGISTGLLLSGSIAAITFAYVARFLAVSFGAVESG
                     LEKVTPNLTAAARTLGRGPIATFFEIHLPLLRPALVTAALLVFVDCMKELPATLILRP
                     FDFETLATTVFMLASLDQLEESALPALTIVAVGFIPVIMLSRTLRDPRIMTAMRASP"
     gene            complement(3025..4983)
                     /locus_tag="AC16A0_05950"
     CDS             complement(3025..4983)
                     /locus_tag="AC16A0_05950"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR3104908.1"
                     /translation="MTDRPLWTPSAARMAEANLTRFVAAANARHGLRLTGFRDTLRFS
                     VEQPEAFWSLLWDFCGVRAETRGSRVLVDGGRMPGARFFPDARLNYAENLLVKSDDSD
                     ALVFRGEDKVARRMSWRELNARVAQMHRALAAAGIGPGDRVCAVVPNMPESIVSFLAV
                     ASLGAIWSSCSPDFGERGILDRFGQIEPRLLITCDAYYYAGKTIRMADKIGNVLKELK
                     SVTQAVVIDYAGEAESVAAALPGAASFQAFLAGHDEGPIAFAQMPFDHPLYILYSSGT
                     TGIPKCIVHRAGGILLKHLCEHVLNTDTKASDRLFYFSTCGWMMWNWLVTGLATGATL
                     LLYDGSPFHPSERVLFDFADAEGMTIFGTSAKYIDAVKKTGWQPKLTHRLPALRSMLS
                     TGSPLSAESFDFVYSGIKADLHLGSISGGTDICGCFVGGNPFEPVWRGEIQGPMLGMA
                     VDVYDETGRPLRNVKGELVCVKPFPSMPVMFWNDPDGSKYHNAYFARFDNIWCHGDFA
                     EITGHDGLIIHGRSDATLNPGGVRIGTAEIYAQVEQIPDVVEAIAVGQDFDNDVRVIL
                     FVRLRQGAVLDDTLVAAIRKKIRDGASPRHVPAKILAVADIPRTKSGKITELAVRDVV
                     HGREVKNKEALANPEALDLFRDLPALRS"
     gene            5080..6879
                     /locus_tag="AC16A0_05955"
     CDS             5080..6879
                     /locus_tag="AC16A0_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3104909.1"
                     /translation="MQRMHVLAGAGLAALVLLLAGWMIFAPGWVTARLQEAIRVQLGR
                     ELKVTGGAHLNLSPLSVQLDGLRLEDPAAPDDALVTAKSARIPISFMGLVGRNIDLTR
                     ATLVDPEFAFLVNEKGDANWALPAPKTPEAIRLDLENASIRFFDARIGQSFTTSGGTL
                     AADITADGGLAVSGTTVVKDRLVKTEATLKSLDRVHEDGSPFQLNFDAPDLGVTFEGR
                     LATAKTLSLVGTVSLSGTSLHQALDWMGVDTGRDGEPGFTVSGALETAGRAFAVKSAE
                     FSLGNTRMSGDMSIDFRSERPRFDGLLSATDLDLALFVPATGSSADDWGRNPLGLGAL
                     RELDATIAIKADSLRYAELTAGPSQINLTLADGQLDSQINMSGLAGGAATLQVTADAT
                     TSPSVLGLGFKAEAVDLQSLLAPFGISWISGRGNLVALVSGQGATQQELIGALKGEAE
                     ASIADGALTGLDIASLFAAASQRIVEGWAGQQGAATGFATLTARATIADGIATMSNLA
                     LSTPTLTLTGSGDVDLLRRSLDLRVDPRVLAADGTSSGLPVSLRVSGPWAAPRIYPDI
                     ADILSKPEQAYERLRAMGLQQQQPQLPTPAMPN"
BASE COUNT         1158 a         2410 c         2298 g         1187 t
ORIGIN      
        1 cggtcgctgc ctgccaccga gatcctgctt gaaggcggcc gcggcgtgac cgcggcggtg
       61 caggtgaccg tcgtgccctg gcatggtgga ccggcgcgcc gcgccgtgct gcgccacgcc
      121 gacctgccac gtggcgaagc cctggcccag aaaaccgggc cggccgaggc cgaccaggcc
      181 ggcgctccgc cgctgcagac ggccgagatc atccagcttc cgcagcgcaa cggccatgcc
      241 gaggccgacg acgagctgcg cgccatcctc gacaccgcca gcgacggcat catcacgctc
      301 gatgcgcagt cgcgcattca cagcttcagt gcaggcgccg aggcgatctt cggctaccgg
      361 atcgccgagg tggcgggaaa gtccttcgcc gagcttttcg tccccgaaag ccgccgggtc
      421 ttgcgcgact atctcgcggc actgcagggc cccggtcttg ccagcgtctt caacgatggc
      481 cgcgaggtgc aggcggtcgt caagcagggc ggcacggttc cgcttttcct caccatcgga
      541 cgccttcagg cgccgaagtc gcaggccgcc ttctgcgccg tggtccgcga catcacccag
      601 tggaagaaga ccgagtccga actgcgcgag gccaaagaaa gggccgaggc gaccagccgg
      661 cagaagtcgg agttcctggc gcgcatcagc catgaactcc gcacgcccct caacgccatc
      721 atgggattct cggaagtgat gcggctggcc cgcttcggcg agatcgagaa cgaaagatat
      781 cgcggctatg tcaacgacat ccatgccagc ggcagccacc tcctgtcgct gatcaatgac
      841 ctgctcgaca tcgccaaggt cgaggcgggc aagctcgagc tcaacttcac cgccgtcaat
      901 cttggcgagg tcatcgacca cgcgatgaag atgctgcagg aagaggcgac atccgcgcgc
      961 gtggtcctgc gcaaggcctg gccgcaaggg ctgcccaatg tggtggccga cctgcggtcg
     1021 atgcggcaga tcatgctcaa tcttctgtcc aacgccatta agttcaccga tcccggcggc
     1081 gaggtgatcg tctcgggcca gctgaccgag gccggagagc tgaagctgcg cgtcaaggac
     1141 accggcatcg gcatggatgc cgatcagctg cgccatgcgc tggagccctt ccggcgcgtg
     1201 ttgacggaag gccgcgaggc acagggcacc ggacttggcc tgccgctgac caaggcgctg
     1261 gcggaagcca atcgcgccgc cttcgcgatc agcagcgaac cgcgcaaggg cacgctggcc
     1321 gagatcacct tccccaccac ccgcgttctg gccgaatgac ggaggcgagc ctgccccggc
     1381 ggcggcacgg ccagcatctt gcgcccggca gccggttcct gcgcgtggct gcgctggtca
     1441 tcggcgggct tctggcgctg ccggtcatca ccatcatctt tcttgccgcg acgcccgcag
     1501 agaacatctg gccgcatctg gttgccaccg ttctgcccgg ttacatctgg cagaccgcgc
     1561 tgctgcttgc aggcgtcggt gccatcacct tcgttgtcgg aacggcggtc gcctggctgg
     1621 tcacggcctg cagctttccg ctccggcggg tatttcaatg ggcctcgctc atgcccatgg
     1681 cgatgccggg ctacatcgtc gcctatacct atgtcgacct gttgagctac gcaggccccg
     1741 tacaaacctg gctgcgcggc ctcttcggct ggaagacgcc cgccgactac tggttccccg
     1801 aaatccgctc catgggcggc gccatcattg tgctgtcgat ggtgctctat ccctatgtct
     1861 tcctcaccgc gcgggcgagc ttcatccgcc agccaggcat gcagatggaa gtggcgcgcg
     1921 cgctgggcca gaccgcctgg ggcgccttcc gccgcgtggc gctgccgctg gcccggccgg
     1981 ccatcgccgt tggtgtaagc ctcgcactga tggaatgcgt caacgacatt ggcgccgccg
     2041 gcttcttcgg cgtgcgcaca ttgacgctgg ggatctatac cacgtggctc tcgcagggaa
     2101 atctcggcgg ggcggctcag atcgcggcgg tgatgctggt cttcgtcttc gccctgctgt
     2161 ggatcgaacg cgccgcccgg cggcagcgca gcttcgtcat gccgacgcgc cgtccgcgcg
     2221 cggccgaccg cgtgcggctg cgcggtatca ggggctggat cgccttcgcc gtatgcctga
     2281 tcccgatcgt catcggcttc atcgtgcccg cgctgaccct ggtgaatttc gccctccacc
     2341 accttgatca ggcggtgtcg tcatccttct tcaaggccgc ctcgcactcc ctggtgcttg
     2401 ccaccaccgc cgcggcgatt gccgtctgcc tgggcctgat cctcggctac tcgaaccgga
     2461 tggcgcccgg cgggctgacg cgcaaggtga tccgcctggc cagcatgggc tatgcaatcc
     2521 ccggcacggt gcttggcatc ggcgtgctca tcccgcttgc cgccttcgac aacgccatcg
     2581 acaccctgat gcgcgaccgg ttcggcatct cgaccggact ccttctgtcg ggctcgatcg
     2641 ccgcgatcac cttcgcctat gtggcgcggt tcctcgcggt gtcattcggc gccgtcgaga
     2701 gcggactgga aaaggtcacg cccaacctca cggctgcggc gcgaacgctg gggcgcgggc
     2761 cgatcgccac cttcttcgaa atccacctgc cgctgctgcg tccggcgctg gtcacggccg
     2821 cattgcttgt cttcgtcgat tgcatgaagg aattgccggc cacgctgatc ctgcgcccct
     2881 tcgacttcga gaccctggcc acgacggtct tcatgctggc ctcgctcgat cagctcgagg
     2941 agagcgccct gccggccctc accatcgtgg cggtcggatt cattccggtc atcatgctgt
     3001 cgcgcaccct gcgcgacccg cggatcatga ccgcaatgcg ggcaagtccc tgaacaggtc
     3061 aagcgcctcg ggattggcga gcgcttcctt gttcttgacc tcgcggccat gcaccacgtc
     3121 gcggaccgcc agttcggtga tcttgcccga cttggtgcgt gggatgtcgg cgaccgcgag
     3181 gatcttggcc ggcacatggc gcggcgaggc gccatcgcgg atcttctttc ggatcgcggc
     3241 aaccagcgtg tcatccagca cagcgccttg cctcaaccgc acaaacagaa tcacgcgaac
     3301 gtcgttgtcg aagtcctggc ccacggcaat ggcctcgacg acatccggaa tctgttcgac
     3361 ctgggcatag atctccgccg tgccgatccg cacgccgccc ggattgaggg tcgcatcgga
     3421 ccggccgtga atgatcaatc cgtcatgacc ggtgatctcg gcgaagtcgc cgtggcacca
     3481 gatgttgtcg aagcgggcaa aataggcgtt gtgatacttg gacccgtctg gatcgttcca
     3541 gaacatcacc ggcatcgacg ggaagggctt cacgcagacc agctcgccct tcacgttgcg
     3601 cagcgggcgg cctgtctcgt catagacatc gacggccatg cccagcatgg gcccctggat
     3661 ctcgccgcgc cagaccggct cgaagggatt gccgccgacg aagcagccgc agatgtcggt
     3721 gccgcccgag atcgagccga gatggagatc ggccttgatg ccggaataga cgaagtcgaa
     3781 gctttcggcc gaaagcggcg agccggtgga cagcatgctg cgcagcgccg gcaggcggtg
     3841 cgtgagcttc ggctgccagc cggtcttctt gaccgcgtcg atgtacttgg ccgacgtacc
     3901 gaagatggtc atgccctcgg catcggcgaa gtcaaacagc acgcgttccg aggggtggaa
     3961 tggcgagccg tcgtagagca gcagcgtggc acccgtggcg aggccggtga ccagccagtt
     4021 ccacatcatc cagccgcatg tggaaaaata gaagagccgg tcggaggcct ttgtgtccgt
     4081 attcagcaca tgctcgcaca gatgcttgag caggatgccc ccggcgcgat gaacgatgca
     4141 tttgggaatg cccgtcgtgc cggacgaata gaggatgtag agcgggtgat cgaagggcat
     4201 ctgcgcgaag gcgatgggac cttcgtcatg accggcgagg aatgcctgga agctcgccgc
     4261 tcccggcagc gccgcggcga cggactcggc ctctcccgca tagtcgatca ccacagcctg
     4321 tgtgaccgac ttcagttcct tcagcacatt gccgatcttg tccgccatgc ggatggtctt
     4381 gccggcatag taataggcat cgcaggtgat cagcagccgg ggctcgatct ggccgaagcg
     4441 gtcgaggatg ccgcgctcgc cgaagtcggg cgaacaggag gaccagatcg cgccaagcga
     4501 cgcgaccgcc agaaagctca cgatggattc cggcatgttg ggaaccaccg cgcagacgcg
     4561 gtcgccgggg ccgatcccgg cggcggccag cgcccggtgc atctgcgcga cgcgggcgtt
     4621 cagctcccgc cagctcatgc ggcgcgcgac cttgtcttcg ccgcggaaga ccagcgcgtc
     4681 gctgtcgtcc gacttgacga gcaggttctc ggcatagttc agccgggcgt cgggaaagaa
     4741 gcgggcgcct ggcatcctgc cgccatcgac gagcacgcgg ctgccgcgcg tctccgccct
     4801 gacgccgcag aaatcccaca agagcgacca gaaggcctcg ggctgttcca cggagaaccg
     4861 cagcgtgtcg cggaaccccg tgagccgcag cccgtggcgg gcattggcgg ccgcgacgaa
     4921 gcgcgtcaga ttggcttcgg ccatgcgcgc ggcactgggg gtccacaagg ggcggtcggt
     4981 catgtatgcg ggtctccggt tcggtcggcg gatggtctag tgtcgtcacg aaagggccgc
     5041 aagtcctgcc gaaaagcggc gtttcaacgg gagttcgcga tgcagcgcat gcatgttctg
     5101 gccggcgcag gcctggccgc cctggtcctg ctgctggcag gctggatgat cttcgcaccg
     5161 ggctgggtga cggcccggtt gcaggaggcc atccgcgtcc agctcggccg cgagctcaag
     5221 gtcaccggtg gcgcacatct gaacctctcg ccgctgtcgg tccagctcga cggcctccgg
     5281 ctggaagatc ccgccgcgcc ggacgatgcg ctggtcacgg cgaagtccgc gcgcattccg
     5341 atcagcttca tgggactggt gggtcgcaac atcgacctga cccgcgcgac cctcgtcgat
     5401 ccggagttcg ccttcctggt gaacgaaaag ggcgatgcca actgggcgct ccccgcaccc
     5461 aagacgccag aggccatccg gctggacctg gagaatgcct cgatccgctt cttcgatgcc
     5521 cgcatcggcc agagcttcac gacatcgggc ggaacgctcg ccgccgacat cacggcggac
     5581 ggcggcctcg cggtttccgg caccacggtg gtgaaggaca ggctggtcaa gaccgaggcc
     5641 acgctgaagt cgctggaccg cgtgcacgag gacggttcgc ccttccagct caacttcgat
     5701 gcgccggatc ttggcgtgac cttcgagggc cggctggcca cggcgaagac gctgagcctc
     5761 gtcggcacgg tgtcgctgag cggcaccagc ctgcaccagg cgctggactg gatgggtgtc
     5821 gacaccgggc gcgacggcga gccgggcttc acggtgtccg gcgcgctcga gacggcaggg
     5881 cgcgccttcg ccgtcaagtc ggcggaattc agccttggca acacgcggat gtcgggcgac
     5941 atgagcatcg acttccggtc ggagcggccg cgtttcgacg gcctgctctc cgccacggat
     6001 ctcgaccttg ccctcttcgt gcccgcaacc ggcagcagcg cggacgactg gggccgcaac
     6061 ccgctggggc tgggcgccct gcgcgaactg gatgccacca tcgccatcaa ggccgacagc
     6121 ttgcgctatg ccgaactcac cgccggcccg tcgcagatca acttgacgct cgccgacggg
     6181 cagctcgaca gccagatcaa catgtccggc ctggccggtg gcgctgcaac gctgcaggtc
     6241 accgcggatg cgaccacgtc tccttccgtg ctcggcttgg ggttcaaggc cgaggctgtc
     6301 gacctccagt cgctgctcgc cccgtttgga atctcctgga tcagcggacg cggcaacctg
     6361 gtggcactgg tctcgggcca gggcgcaacg cagcaggagt tgatcggcgc gctcaaggga
     6421 gaggcggaag ccagcatcgc cgatggcgcg ctgacgggac tcgacatcgc atcgctcttc
     6481 gcggccgcga gccagcgcat cgtggaggga tgggccggcc agcaaggtgc ggcgaccggc
     6541 ttcgccacgc tcacggcgcg cgccacgatt gccgacggca ttgcgaccat gagcaatctt
     6601 gcgctcagca ccccgaccct caccctgacc ggcagcggcg atgtggatct gctgcgccgg
     6661 tcgctcgacc tgcgcgtcga cccgcgagtg cttgcagcag acggcacgag cagcggactg
     6721 ccggtctcgc tgcgcgtctc cggcccctgg gccgcgccgc gcatctaccc cgacattgcc
     6781 gatatcctca gcaagccgga gcaggcctat gaacgcctgc gcgccatggg acttcagcaa
     6841 cagcaaccgc agcttccgac cccggccatg cccaattgaa tgcgcccccg aaggcaggca
     6901 acatgacgcc atgatccgcg tacagcagca acctttcgac gccggccagg aactcgccgc
     6961 cctcaaggcg ggacgtacca gcattggcgg caccgtgatg ttcctcggca ccgtgcgcga
     7021 catctcggat ggctctgaca tccgtgccat gac
//
LOCUS       DBNGVR010000278         6063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3244504, whole genome shotgun sequence.
ACCESSION   DBNGVR010000278 DBNGVR010000000
VERSION     DBNGVR010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3244504"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="AC16A0_05960"
     CDS             complement(<1..578)
                     /locus_tag="AC16A0_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3104910.1"
                     /translation="MTASAALGVLPEWNLADLYAAPDAPEFKADMDRGEADARAFAAR
                     YRGKLATLPGAELAQALKEYEALSDLLGRTGSYAQLYYVGDTTDPARGKFYGDVNARM
                     TDIYALLLFFELELNRIDDAALNASMQVPALAHYRPWIENLRMEKPYQLDDRIEELFL
                     EKSQTGAMAFNRLFDETIAGLRFVVDGEDLAL"
     gene            complement(684..2495)
                     /locus_tag="AC16A0_05965"
     CDS             complement(684..2495)
                     /locus_tag="AC16A0_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3104911.1"
                     /translation="MKPGKILLSLVSASGLALGFAAGWAGRSGWAEAIWGGAAVIALA
                     ALLIQILKSLGRGEFGLDIVAALSMSAAIAFGENLAAAVVALMYAGGQLLEDYASSRA
                     TAEMKALLDRAPKTALRYRDGELESCGIDDLRPGDRILVRQGDIVPADGRVQAGGALL
                     DLSALTGESVPVKAAADHEIPSGALSLDMAFDLVVTKTAAESAYSAIVRLVREAQTAK
                     APMVRLADRYAIWFLLLTVIIAATAYFMSGDRLRLLAVLVVATPCPLILAVPVAIIAG
                     ISRAAKQGVLLKGGPVLEAMAKARVLVIDKTGTLTHGRAELTGIDAQPGWKADGILSL
                     AASLDQASNHAMAHSIVAAAHLRHLKLTQPRKARETAGEGITGWVGPHRVHVGGDGFV
                     RRLLKRKHLPRGAAQTGAVTVAVAVDGKLAGHLIMADTLRDDAPAALSRLRQAGIERI
                     VLASGDSRAVTEKIAEALRLDDVRAELTPAGKVDIVIAERRHGTVLMAGDGVNDAPAL
                     AAADVGIAMGARGSAASAEAADAVLLVDRLERIADAVEISRRARTIALESVTIGLGLS
                     VVAMVVAALGYLSVIQGALLQEAIDLAVILNALRALR"
     gene            2713..4209
                     /locus_tag="AC16A0_05970"
     CDS             2713..4209
                     /locus_tag="AC16A0_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3104912.1"
                     /translation="MASRVLIVEDDPAQRRILEELVKRFGFDPIAVDGGAKGLDALRA
                     SSGGTIELVILDLNMPGMDGLEFLERVQGIRGDLPVIVQTAQGSIETVIKAMRAGADD
                     FVVKPISPERLKVSMQNLLKVNALTEEVKRLNKKVGGALSFDDLIASSPAMANVLRLG
                     RRGAQSNIPILIEGESGVGKEMIARAIQGESDRSGRPFVAVNCGAIPENLVESILFGH
                     ERGAFTGATSRHLGKFQEADGGTLFLDEVAELPLDMQVNLLRAIQEGEIDPVGARKPV
                     KVNIRLVSATNRNMIEMVKAGRFREDLYYRLNVFPIMVPPLRDRKEDIPALVDHFITR
                     FAAEEGRKVRGIAPDALALLRAYAWPGNVRQLENTVFRAIVLCEGDTLGIEDFPQIAA
                     LVDGYEVKIPAAPAAPPKPVLPQGTAAIAGSARSSGGAVSDGMALGIPVVTEGGHMRK
                     LEDVEADMIRMALHRYRGQMSEVARKLGIGRSTLYRKMKELGLEEAAS"
     gene            4316..>6063
                     /locus_tag="AC16A0_05975"
     CDS             4316..>6063
                     /locus_tag="AC16A0_05975"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3104913.1"
                     /translation="MSRLALKIGIAALAIVVAAGAAEASERTKKKKKGFFAALFGEST
                     NTQRKKAKKNRRFLFGGNDDVEIITGSESDITSGRSDDDPEPSPGLGMGNLTYVPDKL
                     ALLGNAKFTEPRPADPAASAIMDALASDDAGIRILPEARDAILDHYRSTGFKPVWLTG
                     QSLQPRAESLLKLLAAAGDEGLDPANYLPPALTSFDAPLPSWDAAAMARLDIGLTAMA
                     IKYARHASGGQFDPRRLSAYNDVEPAWVPASQALKVLAWSPFPVEYLNSLHPQLEAYA
                     ALKKALAESRDALATPAPEPIPDGKIIRIGMSDERMPEIRARLAEKGFGPEGDYIPVD
                     PFVLDTEFSARLKTYQISAGVKASGLVGPQTIRALNGEETINYAAAILNNMERLRWLP
                     KDLGKRHVFVNQPAFSVRVMDSGKETWNSRVIVGKPHTQTSVFHDEMETVVFNPSWGV
                     PQSIIANEYLPKLRRDPGYLDRIGFKVVNQKGKVVSSRSVNWRAYGRKIPYGIHQPPG
                     SDNALGELKFLFPNSHDIYMHDTPNRELFEKDVRAFSHGCVRVQNPREFAAVLLGWDS
                     AKIDEYVSTHGSENVKL"
BASE COUNT         1167 a         1974 c         1878 g         1044 t
ORIGIN      
        1 tcgagcgcca gatcttcgcc atccaccaca aagcgcaggc cggcgatggt ctcgtcgaaa
       61 aggcggttga atgccatggc acccgtctgc gacttctcga ggaagagctc ctcgatcctg
      121 tcatcgagct gatagggctt ctccatgcgg agattctcga tccacggacg gtagtgcgcc
      181 agcgcgggta cctgcatgga tgcattcagc gccgcgtcat cgatgcgatt gagctcgagt
      241 tcgaaaaaca gcagcagcgc atagatgtcg gtcatgcgcg cattgacgtc gccatagaat
      301 ttcccgcgcg ccgggtcggt cgtgtcgccg acatagtaga gctgcgcata tgagccggtg
      361 cgccccagca gatcggacag cgcctcgtac tccttcaacg cctgcgcaag ctcggcgccc
      421 ggcagcgtgg cgagcttgcc acgatagcgg gcggcaaagg ccctggcatc ggcctcgccg
      481 cgatccatgt cggccttgaa ctcgggggca tccggcgcgg catagaggtc ggcgagattc
      541 cattcgggca gcacgcccag ggcggcagaa gcggtcattt gcggtccttc atcagatttc
      601 agccagatat ggcccatcgc ccgaccttcg gcaacccgag cctcccccgt gatgacaatg
      661 aattcatgtc ggcagcgtgc cgttcagcgc agcgcccgca atgcgttgag gatgaccgcc
      721 aggtcaattg cctcctgcag caatgccccc tggatgaccg aaagatagcc caaggcggca
      781 acgaccatcg cgacaaccga aagcccaaga ccgatggtca cgctctccag ggcaatcgtg
      841 cgtgcacggc gcgaaatctc cacggcatcg gcgatgcgct ccagacggtc gacgaggaga
      901 acggcatcgg ctgcttccgc cgaagccgcc gaaccacgcg cacccatcgc gatgccgaca
      961 tcggcggccg ccagcgccgg cgcatcattg accccgtcgc cggccatcag caccgtgcca
     1021 tggcgccgct cggcgatcac gatgtcgacc ttgcccgccg gcgtgagttc ggcgcggaca
     1081 tcgtcaagcc gcaacgcctc ggcgatcttc tccgtcaccg cgcggctgtc gccggaggcg
     1141 agcacgatcc gctcgatacc ggcctgccgc agccgcgaca gggctgcggg cgcatcgtca
     1201 cgaagcgtat cggccatgat caggtggccc gcgagcttgc cgtcgacggc aaccgcgaca
     1261 gtcacggccc ccgtctgcgc cgcgccgcgc ggcaggtgct tgcgcttcag cagccggcgg
     1321 acgaaaccgt ccccgccaac atggacgcga tgcggcccga cccatccggt gatcccctcg
     1381 cccgccgtct cgcgcgcctt gcgcggctgg gtcagcttca ggtggcgcag atgggccgcg
     1441 gccacgatgg aatgcgccat ggcatggttc gaagcctgat ccagcgatgc ggcaagcgac
     1501 aatatcccgt cggccttcca tccgggctgg gcgtcaattc cggtcagttc ggcccggccg
     1561 tgggtcaggg ttccggtctt gtcgatgacg aggaccctgg cctttgccat tgcttcgagc
     1621 actggcccgc ccttgagcag caccccttgc ttggcggccc ggctgatccc cgcaatgatc
     1681 gcgacgggca ccgccaggat gagcggacag ggcgtggcca caaccaggac ggcaagcagc
     1741 cgcaaccggt ctccggacat gaaataggcc gttgccgcga tgatcacggt gagcagcagg
     1801 aaccagatgg cgtagcgatc cgccaggcgc accatgggcg cctttgccgt ctgggcctcc
     1861 cggaccagac gcacgatggc cgagtaggca ctctcggccg cggtctttgt caccacgaga
     1921 tcgaacgcca tgtcgaggct gagggcgccg ctgggaatct cgtggtccgc cgcggccttg
     1981 accgggaccg actcgccggt cagcgccgac aggtcaagca gtgccccgcc cgcctgaacc
     2041 cgtccatcgg ccggcacgat gtcgccctgg cgcacgagga tccggtcgcc cggccgcagg
     2101 tcatcgatgc cgcaggattc aagttcgcca tcgcgatagc gcagggccgt cttgggggcg
     2161 cggtccagca gcgccttcat ttcggcggtc gcccggctgg aggcgtagtc ctcgagcagc
     2221 tggccgccgg catacatcag cgcgacgacc gccgcggcaa gattttcgcc gaaggcgatg
     2281 gccgcggaca tggacagggc cgcaacgata tcgagcccga attcgccgcg gccaagcgat
     2341 ttcaggatct ggatcagcag ggccgccagc gcgatgacgg ccgcgccgcc ccagatggcc
     2401 tcagcccagc cactccggcc tgcccagccc gcggcgaaac cgagggcaag cccggatgcc
     2461 gacaccagag acagcagaat cttgcccggt ttcaccgctt tatccttgtt atggcatcac
     2521 gttagcatag gagcgatgcc cgcaactgat cctggtcaag acaccgggaa tgttcctaaa
     2581 cgtgttaggt cattaagtct gcctttaccc ttaactgcca gactcgcgat cctcagctgg
     2641 cacagatcca agccagctcg aaaccgagac cagcgccaac acacctgatt tcaaaggacc
     2701 ggtcgttcca gcatggcttc ccgcgtattg attgtagaag acgatccggc acagcgccgc
     2761 atcctcgaag aactggtcaa gcgttttggt tttgatccga ttgccgtcga tggcggcgcc
     2821 aaaggcctcg atgccttgcg cgccagttcc gggggcacca tcgaacttgt gattctcgat
     2881 ctcaacatgc ccgggatgga tgggctggag ttcctcgaac gggtccaggg catccgcggc
     2941 gatctgcccg tcatcgttca gacggcccag ggctcgattg agacggtgat caaggccatg
     3001 cgggccggcg ccgacgactt cgtggtcaag ccaatctcgc cggaacggct caaggtatcg
     3061 atgcagaacc ttctgaaggt caatgccttg accgaggagg tcaagcggct gaacaagaag
     3121 gttggcggag cgctgtcttt cgatgacctc atcgcgtctt ccccggccat ggcgaacgtc
     3181 cttcggcttg gtcgccgcgg ggcacagtcc aatattccga ttctcatcga gggcgaatcg
     3241 ggggtcggca aggaaatgat cgcccgcgcc atccagggtg agagcgacag atccggacgc
     3301 ccattcgtgg ccgtcaactg cggcgcaatt ccggagaatc tcgtcgagtc gattctgttc
     3361 ggccatgaaa gaggtgcttt caccggtgcg accagccgtc acctcggcaa gttccaggaa
     3421 gccgatggcg gcacgctgtt cctggatgaa gtcgccgaac tgcccctcga catgcaggta
     3481 aatctgctgc gcgcaattca ggaaggtgaa atcgatccag tcggcgctcg caagccggtg
     3541 aaggtcaata tccgcttggt gtcggccacc aatcgcaata tgatcgaaat ggtgaaggcc
     3601 ggccggttcc gcgaagatct ttactaccgc ctcaacgtct tccccatcat ggtaccgccg
     3661 ctgcgcgacc gcaaggagga catcccggcg ctggtcgacc atttcatcac ccgctttgcg
     3721 gccgaggaag gccgaaaggt gcgcggcatc gcgccggatg ccctcgcgct gctgcgcgcc
     3781 tatgcctggc caggcaatgt ccggcaactc gagaacaccg tgttccgcgc catcgtgctg
     3841 tgcgagggcg atacgctggg gatcgaggac tttccgcaga ttgcggccct ggtcgacggt
     3901 tacgaggtga agattcccgc agcgccggcg gccccgccaa agccggttct gccgcagggc
     3961 acggctgcaa ttgccgggtc cgcgcgctcg tccgggggcg cggtcagcga cggcatggcg
     4021 ctgggcattc cggttgtcac cgagggcggg cacatgcgca agcttgaaga tgttgaagcc
     4081 gacatgatcc gcatggcctt gcatcgctat cgcggccaga tgtctgaagt tgcacgaaaa
     4141 ctcggcatcg gaagatcgac cctctatcgc aagatgaagg aattggggct tgaggaagcc
     4201 gccagctgac accgcgagac gcacactgca atgtgagggt gtggatatct ctttcggttg
     4261 aactcctccg gcatgaccat taaggattgg gctcaagagg gttaagggag tcccgatgtc
     4321 caggctggcc ttgaagatcg gcattgccgc gctcgccatt gtcgtggctg caggcgcagc
     4381 cgaggccagc gaacgcacca agaaaaagaa gaagggcttc ttcgccgcac tgttcggcga
     4441 gtcgaccaat acgcagcgca agaaggccaa gaagaaccgc cgcttcctct tcggcggcaa
     4501 cgacgatgtc gagatcatca ccggatccga atcggacatc accagcggcc gcagcgacga
     4561 tgatccggag cccagcccgg ggctgggcat gggcaatctg acctatgtgc ccgacaagct
     4621 ggcactcctg ggcaatgcga agttcaccga gccgcgtccg gctgatccgg cggcctcagc
     4681 catcatggat gcgctggcaa gcgacgacgc gggcattcgc atccttcccg aggcccgcga
     4741 cgccatcctc gaccactacc ggtcgacagg attcaagcct gtctggctga cgggccagtc
     4801 cctgcagccg cgcgcagaga gtctcttgaa gctgctggcg gcggccggag acgaagggct
     4861 cgatccggcg aactacctgc ctcccgcctt gacctccttc gatgcgcccc tgcccagctg
     4921 ggacgcggcc gcgatggctc gcctcgatat cgggctgaca gccatggcga tcaaatatgc
     4981 ccgacacgca tcaggcggcc agttcgatcc gcgccgcctg tccgcctaca acgatgtcga
     5041 gccggcctgg gtgcccgcca gccaggccct caaggttctg gcctggtcgc ccttcccggt
     5101 cgagtatctg aacagtcttc atccgcagct ggaggcctat gccgccttga agaaggcgct
     5161 ggcggagagc cgcgacgccc ttgccacgcc ggcaccggaa ccgattcccg acgggaagat
     5221 catccgcatc ggcatgtccg acgagcgcat gcccgagatc cgcgcgcgcc ttgctgagaa
     5281 gggatttgga ccagaaggcg actacatccc ggttgatccc tttgtcctcg acaccgagtt
     5341 ctccgcgcgc ctcaaaacct atcagatcag cgccggcgtc aaggcctcgg gcctcgtcgg
     5401 cccccagacg atccgggccc tgaacggcga ggaaacgatc aactatgccg ccgcaattct
     5461 gaacaatatg gaacgtctgc gctggcttcc caaggacctg ggcaaacgcc atgtcttcgt
     5521 caaccagccc gccttttccg tgcgggtgat ggacagcggc aaggagacct ggaacagccg
     5581 cgtcatcgtt ggaaagccgc acacccagac atcggtgttc cacgacgaga tggaaaccgt
     5641 ggtgttcaac ccctcctggg gcgttccgca atcgattatt gcaaacgagt acctgcccaa
     5701 actgcggcgt gatccgggct atctcgaccg gatcggcttc aaggtggtga accagaaggg
     5761 caaggtcgtc agctcgaggt ccgtcaactg gcgcgcctat ggcaggaaga ttccctacgg
     5821 gatccatcaa ccgcctggaa gcgataacgc attgggtgaa ttgaagttct tgttccccaa
     5881 cagccatgac atctacatgc atgacacgcc gaaccgggag ctcttcgaga aggatgtccg
     5941 ggcattcagc catggctgtg tccgggtgca gaatccccgg gaatttgccg ccgtgctctt
     6001 gggttgggat agtgccaaga tcgatgagta cgtctccacg catggcagcg aaaacgtgaa
     6061 gct
//
LOCUS       DBNGVR010000279         3237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5404632, whole genome shotgun sequence.
ACCESSION   DBNGVR010000279 DBNGVR010000000
VERSION     DBNGVR010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5404632"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="AC16A0_05980"
     CDS             <1..324
                     /locus_tag="AC16A0_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HIR3104914.1"
                     /translation="AFAFGWTPCIGPVLAMILTLAATEQDMARGAGLLAVYSAGLGIP
                     FLLAAAGIGTCLGFFRRFRSQMRNVERAMGLLLLLTGVMFLTGYMQAFSYWLIEQFPW
                     LATIG"
     gene            complement(287..637)
                     /locus_tag="AC16A0_05985"
     CDS             complement(287..637)
                     /locus_tag="AC16A0_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HIR3104915.1"
                     /translation="MKLSDLDSLPRDESGPVFAEPWEAQAFAMAVKLNDAGVFTWSEW
                     AATLGAEIRAEPDRGYYGSWLAALERLVEAKGLIGTSERQERIADWDAAARATPHGQP
                     IDLSRSSPARGTAR"
     gene            complement(624..1274)
                     /gene="nthB"
                     /locus_tag="AC16A0_05990"
     CDS             complement(624..1274)
                     /gene="nthB"
                     /locus_tag="AC16A0_05990"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003580639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HIR3104916.1"
                     /translation="MNGAHDCGGMMGFGAVATERDEPVFHSAWESRAFALMSAVGDIG
                     GWTLDEDRSACESMHPADYAATSYYEHWLHGLQVLLVKHGLVTAEELADGMCRTAPVA
                     KQPTQAPAVWDLVTAPGSYAREPQGPARFAVGDRVRTRRLNPAGHTRLPRYLRGHAGE
                     IVARHGAHVFPDSNAAGRGEDPQWLYTVRFACAEVWGTATRDVIHADLWEPYLEAV"
     gene            complement(1271..1924)
                     /gene="nthB"
                     /locus_tag="AC16A0_05995"
     CDS             complement(1271..1924)
                     /gene="nthB"
                     /locus_tag="AC16A0_05995"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HIR3104917.1"
                     /translation="MNGPQDAGGMMGFGPVVPEPVTPVFHSDWERRAFAITLAMGMTG
                     SWNIDISRHARERIPPAEYWAASYYEVWLKGLERLLAEAGLASVEEIAAGRSLTAARP
                     VTRVATADMIAAILARGGPADRPAAAPPQFAAGDKIRTRNMNPEGHTRLPRYLRGCPG
                     TIAIVHGCHVLPDSSAHGRGDDPHWLYAVRFSASDVFEAPGRDEIVADLWEPYLEAA"
     gene            complement(1921..2541)
                     /gene="nthA"
                     /locus_tag="AC16A0_06000"
     CDS             complement(1921..2541)
                     /gene="nthA"
                     /locus_tag="AC16A0_06000"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR3104918.1"
                     /translation="MPHDGHDLDDDAGAALSPVALRVKALESLLVEKGYVDPAALDVL
                     IETYETRIGPRNGARVVARAWTDPDFADELRRDATAAIASMGYAGRQGEHMVALFNTP
                     ETHNMVVCTLCSCYPWPVLGLPPVWYKSMPYRSRAVMEPRAVLAEFGVTLPEGTAVRV
                     WDSTAEVRYLVIPQRPGGTEGWSAEQLAGLVTRDSMIGTGLVGSPA"
     gene            2604..>3237
                     /locus_tag="AC16A0_06005"
     CDS             2604..>3237
                     /locus_tag="AC16A0_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR3104919.1"
                     /translation="MTKLEGVRACVFDAYGTLFDIHAPLRKLAPEIGPEAENISKLWR
                     QKQLEYAWLRSLMGVHADFWHVTGDALDYALEHYKVHELGLRDEIMALYLKLEAYPDV
                     AEALQAVRNQGKRTAILSNGSPSMLDSAVRHAGVEKLFDAVLSVEDVGIYKPSRRVYR
                     HAMQKLQLHDAPSICYVAANTWDAQAAAQFGFQAVRVDRFGLGEERIPGKP"
BASE COUNT          530 a         1125 c         1042 g          540 t
ORIGIN      
        1 gccttcgcct tcggctggac accctgcatc gggccggtgc tggccatgat cctcaccctc
       61 gccgccaccg agcaggacat ggcgcggggt gcgggcctgc tcgctgtcta ttccgccggc
      121 ctcggcattc ccttcctgct ggcggccgcc ggcatcggga cctgcctcgg cttcttccgc
      181 cggttccgca gccagatgcg gaatgtcgaa cgggcgatgg gactgctgct gttgctgacc
      241 ggcgtcatgt tcctcaccgg ctacatgcag gccttctcct actggctcat cgagcagttc
      301 ccctggctgg cgacgatcgg ctgaggtcga tcggctggcc atgcggcgtg gcgcgcgcgg
      361 ctgcgtccca gtccgcgatc cgttcctgac gctcggatgt accgatcaga cccttggctt
      421 cgacgaggcg ttccagcgcc gccagccatg agccgtaata gccacggtcc ggctctgccc
      481 ggatctcggc accaagcgtc gcggcccatt cgctccaggt gaagacgccc gcgtcgttga
      541 gcttcaccgc catggcgaag gcctgggcct cccagggctc ggcgaagacg gggccgctct
      601 cgtccctggg cagtgaatcg aggtcagaca gcttcaaggt atggctccca cagatcggcg
      661 tggatcacgt cgcgcgtcgc cgttccccac acttcggcgc aggcgaaccg cacagtatag
      721 agccactggg gatcttcccc gcggcctgcc gcattgctgt cgggaaagac atgggcacca
      781 tgccgggcga cgatctcgcc cgcatggccg cgcaggtaac gcggcaggcg cgtatgcccg
      841 gcagggttca ggcgccgggt cctgacccgg tcgccaacag caaagcgggc cggcccctgc
      901 ggttcgcgcg catagcttcc tggcgcagtc accaggtccc agacggccgg tgcctgggtg
      961 ggctgctttg cgaccggcgc ggtgcggcac atgccatcgg cgagttcctc cgccgtgaca
     1021 agtccatgct tcaccagcag tacctgcagc ccatgcagcc agtgctcgta gtagcttgtg
     1081 gccgcataat cggcagggtg catggattcg caggcactgc gatcctcatc cagtgtccag
     1141 ccgccgatgt cgccaaccgc cgacatcaag gcgaaagcgc gcgactccca cgcgctgtgg
     1201 aacacgggct cgtcccgctc cgtggcaacc gcaccgaacc ccatcatgcc accgcaatca
     1261 tgcgcgccgt tcatgccgcc tccaggtagg gttcccagag atcggcgacg atctcgtcgc
     1321 ggcccggtgc ttcgaagaca tccgatgccg aaaaccgcac cgcatagagc cagtgcggat
     1381 catcgccacg gccgtgcgcg gacgaatccg gcagcacatg gcatccatgc acaatggcaa
     1441 tggtgcccgg gcagccgcgc agatagcgcg gcaggcgggt gtggccctcc gggttcatgt
     1501 tccgtgtacg gatcttgtcg ccggccgcga actgcggcgg cgcagcagcc ggtcggtcgg
     1561 ccggccctcc tcgcgctagg atggccgcga tcatgtccgc cgttgcgacc cgcgttaccg
     1621 gcctggcggc cgtcagactc cggccggctg caatctcctc caccgaggcc agcccggctt
     1681 ccgccagcaa ccgctccagc cccttgagcc aaacctcgta gtagctcgcg gcccagtatt
     1741 cggcgggcgg aatgcgttcg cgcgcgtggc gcgaaatgtc gatgttccag ctgccggtca
     1801 tgcccatggc gagcgtgatg gcgaaagccc ggcgctccca gtcgctgtgg aacacgggcg
     1861 tcaccggttc cggcaccacc ggcccgaagc ccatcatgcc gccggcatcc tgcggtccat
     1921 tcatgccggc gaacccacca gtccggtgcc gatcatggaa tcgcgggtca cgagcccggc
     1981 aagctgttcc gcactccagc cttcggtgcc gccgggccgc tgcgggatca ccaggtagcg
     2041 aacctcggcg gtggagtccc agacgcgcac cgccgttcct tcggggagcg tcacgccgaa
     2101 ctcggccagc acggcgcgcg gttccatcac ggcccgcgag cggtagggca tggacttgta
     2161 ccagaccggc ggcaggccaa gcaccggcca cgggtaacag gagcacaggg tgcagaccac
     2221 catgttgtgg gtctcgggtg tgttgaacag cgccaccatg tgttcgccct ggcgtccggc
     2281 atagcccatg gaggcgatcg ccgcggtggc gtcgcgtcgc aactcgtcgg cgaagtccgg
     2341 atcggtccag gcccgtgcga cgacgcgggc cccgttgcgc gggccgatcc gcgtctcgta
     2401 agtctcgatc agcacgtcca gcgcggcggg atcgacatat cccttctcga ccagcagtga
     2461 ttcgagcgcc tttacgcgga gcgcgacggg cgacagcgcc gcgcccgcgt cgtcatcaag
     2521 gtcatgaccg tcatggggca ttttccggac ctcactcttg ggttaaacat agggccaaga
     2581 ggcattgggg ggaagcggcg gccatgacca agctcgaagg tgtaagagcc tgcgtattcg
     2641 atgcctatgg caccctcttc gacatccatg cgccgctgcg gaagctggcg cccgagatcg
     2701 ggccggaggc cgaaaacatc tcgaaactct ggcgccagaa gcagctcgaa tacgcctggc
     2761 tgcgcagcct gatgggcgtc catgccgact tctggcacgt caccggcgac gcgctggact
     2821 atgcgctcga gcactacaag gtgcatgaac tcggcctcag ggacgagatc atggcgctct
     2881 acctcaagct cgaagcctac cctgacgtgg cggaggcgct tcaggctgtc cgcaaccagg
     2941 gcaagcgcac ggcgatcctc tccaacggca gcccgtcgat gcttgattca gcggtgcgcc
     3001 acgccggcgt cgagaaactg ttcgacgccg tgctctcggt ggaggacgtg ggaatctaca
     3061 agcccagccg ccgcgtctac cgccacgcca tgcagaagct gcagcttcac gatgcgccgt
     3121 cgatctgcta cgtcgccgcc aatacatggg atgctcaggc tgcggcgcag ttcggcttcc
     3181 aggccgtgcg cgtagaccgc ttcggccttg gggaagaacg tattcctggc aaacccg
//
LOCUS       DBNGVR010000280         2613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1477576, whole genome shotgun sequence.
ACCESSION   DBNGVR010000280 DBNGVR010000000
VERSION     DBNGVR010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1477576"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1128)
                     /locus_tag="AC16A0_06010"
     CDS             complement(151..1128)
                     /locus_tag="AC16A0_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104920.1"
                     /translation="MAPVLDIRSLSVQFSTAGQPARVLRDIKLSVPRGEIVGIVGESG
                     SGKSTLALAILGLLSANASVTAGQIMLGDVDLLALPPAGLREIRGRRVTMVFQDPMTS
                     FNPVRSIGKQMTDIQFRDKSASIEDKRRKAAAALTRVGIPDAMSQLDNYPFQFSGGMR
                     QRIAIAMGLLQRPELLIADEVTTALDVTLEAQILHLLRELSSEIGCSILFISHNLGAI
                     AEICDRVVVLYAGEVVEQGPIGDIFDRPQHPYTRALIACDPARIAEATRDLPVIPGEV
                     PNLRALPAGCIFAPRCSQAMERCRTEAPVDIEVGRQHVARCHLLCEARP"
     gene            complement(1128..2003)
                     /locus_tag="AC16A0_06015"
     CDS             complement(1128..2003)
                     /locus_tag="AC16A0_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104921.1"
                     /translation="MQRSESIIARPGSARRAWRRVTDDPMGAVGLILVLIVVFCGIFG
                     PWITPFDPLKINVPDKFQAPSLTHLMGTDNLGRDVFSRVIAGSRIALTIGISTIAIAL
                     VLGLILGLVAGYGPRWLDNLLMLIFDSIYSFPTVVLGLTVVTLLGASVTTLVLVVIVI
                     QTPAYARLTRTATLTIKNSEYIQAIESLGASKLRIIFLHILPNVVGPLLIVASMDIPS
                     VVALEAGLTYLGMGIPPPAPSWGRILQEGYSLIRVAPWIVVAGGIPLIITTLGFTFLG
                     ESLRDNLDPKLRRML"
     gene            complement(1990..>2613)
                     /locus_tag="AC16A0_06020"
     CDS             complement(1990..>2613)
                     /locus_tag="AC16A0_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104922.1"
                     /translation="WAALIGIPLGCYSAVRRNSFIDKFTGVISVGTISIPSFVVAIYL
                     ILIFAIALGWLPVIGAGEPGNLQDQIMHLILPAFALGLGWVGYLARIVRASMLEVMGE
                     NHIRTARAFGLPEFKIVSSYALRIAILPTITLLAIAVGGLLSSAVFVEAIFARPGIGK
                     LIVDAANMRNFPVVQGSVLTTVALFALVMPIADLLIAWLDPRVRATL"
BASE COUNT          558 a          787 c          852 g          416 t
ORIGIN      
        1 cgccgacgat ggcgagcgtg ctgccgcgcg caacatcgaa ggaaacgtcg acgaccgcat
       61 cgacatggcg gtttgccagg cccgccactg cggccttcag cctgctggca atggtgtagc
      121 ggacatgcag tccgtggacg gacagcaggg tcatgggcgc gcctcgcaaa gcagatggca
      181 acgcgcgacg tgctgtcgtc cgacctcgat gtctactggc gcctctgtcc ggcagcgctc
      241 catcgcctga ctgcagcgtg gcgcaaagat gcaacccgca ggcaaggcgc gcagattggg
      301 aacctcgcca ggaatgaccg gcagatcgcg cgtcgcctct gcaatccgcg ccggatcgca
      361 ggcgatcagt gcccgtgtgt agggatgctg cggccggtcg aagatgtcgc cgatcggccc
      421 ttgttcaacg acttctccgg catagagcac cacaacgcga tcgcagatct cggcaatggc
      481 cccgagattg tgcgagatga agaggatgga acagccgatt tcagaactca gctcacgcag
      541 caggtgcagg atctgggctt cgagcgtgac gtcgagcgcg gtcgtcacct cgtcggcgat
      601 gagcagctcg ggccgctgga gcaagcccat ggcaatggcg atacgctggc gcattccgcc
      661 ggagaactgg aagggatagt tgtcgagctg cgacatcgca tctggaatgc cgactcgcgt
      721 cagggcggcg gcagccttcc ggcgcttgtc ctcgatgctg gcacttttgt cgcggaactg
      781 gatatcggtc atctgcttgc cgatgctgcg caccggattg aaggatgtca tggggtcttg
      841 aaacaccatg gtcacgcggc ggccgcggat ctcgcgcagt ccggcgggcg gcagagcaag
      901 aagatcgaca tcgccaagca tgatctggcc agccgtgacg gaagcgttgg cggacaggag
      961 gccgagaatt gcaagcgcca gcgtcgactt gccggatcca ctttcgccca cgatgccgac
     1021 gatctccccg cgcggcacgg acagtttgat gtcgcgcagc acccgggcgg gctggcccgc
     1081 cgtgctgaat tggaccgaca ggctccggat gtcgagaacc ggcgccatca cagcattcgc
     1141 ctgagcttcg gatccagatt gtcgcgcagg ctttcgccga ggaaggtgaa gccgagggtc
     1201 gtaatgatca gcggaatgcc gcccgccacc acgatccacg gcgccaccct gatcagcgag
     1261 taaccctcct gcaggatacg gccccagctg ggtgcgggcg gcggtatgcc catgccgaga
     1321 taggtcaggc ccgcctccag agccacgacg gagggaatat ccatgctggc cacgatcagc
     1381 aggggtccga ccacattggg caggatatga aggaagatga tgcgcagctt cgatgcgccg
     1441 agcgactcga tggcctggat gtattccgag ttcttgatgg tgagcgtcgc cgtgcgggtg
     1501 agccgtgcat aggccggcgt ctggatgacg atcaccacca gaaccagtgt ggtgacgctg
     1561 gcgcccagaa gggtcaccac cgtgagcccc aggaccaccg tcgggaagga gtagatcgag
     1621 tcgaagatca gcatcagcag attgtccagc caacgcggcc cgtatcccgc aaccagtccg
     1681 agaatgaggc ccagcaccaa agcaatggcg atggtcgaaa tgccaatggt cagcgcgatg
     1741 cggctgcccg caatcacgcg agagaagacg tcgcggccaa gattgtcggt tcccatgaga
     1801 tgagtaagcg acggcgcctg gaacttgtcg ggcacattga tcttgagcgg atcaaagggc
     1861 gtgatccacg gcccgaagat accgcagaaa accacgatca gcacgaggat caatccgacg
     1921 gcacccatcg gatcatcggt gacccgccgc caggcgcggc gggcggagcc gggacgtgcg
     1981 atgatgcttt cagagcgttg cacggacacg ggggtcgagc caggcaatga gcaagtccgc
     2041 aatcggcatg acgagggcaa agagagccac cgtggtcagc acgcttccct ggacgaccgg
     2101 gaagttgcgc atattggcgg cgtcgacgat cagcttgccg attcccgggc gagcgaagat
     2161 cgcttccacg aagacggcgc tggacagcag gccgccgacg gcgatggcca gcagcgtgat
     2221 ggtaggcaaa atcgcgatgc gcaacgcata ggaactcacg atcttgaact ctggcaggcc
     2281 aaaggccctt gccgttcgga tatggttctc gcccatcacc tccagcatcg aggctcggac
     2341 gattcgcgcc agatagccga cccagcccag tccgagcgcg aatgcaggca ggatcagatg
     2401 catgatctgg tcttgcagat tgcccggttc gccggcgccg atcaccggca gccagcccag
     2461 cgcaatggcg aagatcagaa tgagatagat cgccaccacg aaactcggaa tcgaaattgt
     2521 accgaccgag atcacgccgg tgaacttgtc gatgaaggaa ttccgtctca ctgcggaata
     2581 gcagcccagc ggaatgccga tgagcgccgc cca
//
LOCUS       DBNGVR010000281         4824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1478895, whole genome shotgun sequence.
ACCESSION   DBNGVR010000281 DBNGVR010000000
VERSION     DBNGVR010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1478895"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1250
                     /locus_tag="AC16A0_06025"
     CDS             228..1250
                     /locus_tag="AC16A0_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3104923.1"
                     /translation="MNLRELAAHLGLSQTTVSRALNGFPEVSEETRLRVKAAADLHGY
                     RPSAAARRLATGQSGTLGMIFPGERNMLGDLLFIEFLGGCVESAADLGYDVTLAMARG
                     QQSEEAIYRRTVRSARVDGMILSSPLVTDARPALLDSLGMPFIVHGRTRSDKAYAFLD
                     IDNRGAFEAATRLLVDLGHRRIALINGDPRYNFAADRETGFRDALRARGIAPAPALIS
                     GGPMHEGTGHAQALRLLDSEDPPTAFLCSSLAQAAGVRRACLDRGLRAGQDLALIAHD
                     DRLQEMRAEAFDPPLTTTQSPIGDAGRRIVELLVALLRDPKQVLPAEVWPVDLVVRNS
                     TPAPKP"
     gene            complement(1253..2554)
                     /locus_tag="AC16A0_06030"
     CDS             complement(1253..2554)
                     /locus_tag="AC16A0_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HIR3104924.1"
                     /translation="MKSLNTAKSLRRFQIAGYVSVFLAVGVLGGWSAFASIHGAVIAP
                     ATIVVESNTKRIQHRDGGIVDAILVKDGDHVEAGQDLVVLDSTDNVAELGIIDSVLSE
                     ELAKRARLEAERDEAAEVVFPEQLVSRENDPDIAKLMIGQQKLFAAKRATIQGKVEQL
                     KQQIGQIDEQVQGILAQADAKERQIKLIGEELVGLLDLQAKGLVPNTRVLAMQRERAR
                     LEGERGELIATKASAESRVGEVKLQILQIREETLSETLAQLRETEARIAELNERRVSI
                     KTRLERTTIRAPISGAVYQLSVHTVGGVITPAETLMLIVPESDDLVLQAQVPPSQIDR
                     VTLGQTARVRFSAFDTRVTPEIGAEVTHVAADVSRVDANSPPFYMVQLKIPATELALL
                     GDNRLRPGMPAEAFIQTGARTPLSYMVKPLFDQIAHSWRER"
     gene            complement(2558..4309)
                     /locus_tag="AC16A0_06035"
     CDS             complement(2558..4309)
                     /locus_tag="AC16A0_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HIR3104925.1"
                     /translation="MPQTPPGSPEAQELKAVYRASRGAFISTGIFSCLVNILMLTGPL
                     YMMQVYDRVLASGSVPTLLALSIIVIGLYAYYGFLEYLRSRILVRIGRRVEEQLRGRV
                     FDVVTAHALRRTPGIGAQPVNDLGTIRQYLSGQGPFAFFDMPWMPIYLLIIYLIHWSL
                     GVAAVAAAVAIFTLALISERVTRGPIAEATKSSIKAAQISEESRRNSEALHSLGMLRN
                     LRERWTGVQSAALDQQTVANDAGGILSAASRVLRLMVQSGILGLGAYLAIQHEITGGA
                     MIAASIIMSRALAPVEQAVGSWQQFISYRKARERLSAVLSSIPAEAPPMVLPPPRGKL
                     DVENLIIQLPGVEKPLLHGISFSLQPGEGLGVIGPTGAGKSTLARALVGVLQPTRGHI
                     RLDGATLDQREPDALGRAIGYLPQDLQIFDGTAAQNISRFDPQPDSGKIIEAAKRANV
                     HDMIMRLPEGYNTQLGENGSRLSTGQRQRLALARALYGDPAVFVLDEPNSALDAEGEA
                     ALDGAIRHAMARGATVVVVAHRPSALAAIPKMLVLADGKMAALGPREEVLRKVLVKEP
                     AKSSAETGPTLVQTGRT"
     gene            complement(4387..>4824)
                     /locus_tag="AC16A0_06040"
     CDS             complement(4387..>4824)
                     /locus_tag="AC16A0_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104926.1"
                     /translation="IPLETSMRFILSIPLLAVVILAYVLLAQSGSMLQPDSTFYDTVL
                     PSGAEIFFLVGDVFVMAGLVALFIEILKAARYAGNTILDHMLSTAAFIGALIAFLLVP
                     SCGTVTFFLLMVMTLIDVVAGFSVSIVAARRDFTVSQGNGTLA"
BASE COUNT          914 a         1512 c         1499 g          899 t
ORIGIN      
        1 ttgtgaacct taaccctgcg cttcgacgag gtgttcccct tcttcttcaa agcgctttga
       61 atacacttct ccttaacaga ttgaaaggtt gcgtcaagac tcaaagcgct tgcagcctta
      121 tcttttctat tgtgcaccgc gccatatctg ctgcggcgca gcgcattggg ggcgcaaacc
      181 ggtaaaacga tttgaaagcg ctttgacttt gtgtcacaaa caagcgcatg aaccttcgcg
      241 aactcgcggc ccacctcgga ttgtcacaga cgaccgtcag ccgggcgctg aacggctttc
      301 cggaagtttc ggaggaaacc cggctccgcg tcaaggccgc ggccgatctg catggctatc
      361 gacccagcgc cgccgcgcgt cgactggcga ccggtcaatc gggaacgctt ggcatgatct
      421 tcccgggcga gcgcaacatg ctcggcgatc tgctcttcat cgagttcctg ggtggttgcg
      481 tcgagtccgc cgccgacctc ggctacgacg tcacccttgc gatggcccgc gggcaacaga
      541 gcgaggaggc catctaccgg cggacggttc gcagtgcccg tgtcgatggc atgatcctct
      601 cgagccctct ggtcaccgac gcccgacctg ccctgctcga cagccttgga atgcccttca
      661 tcgtccatgg ccggacccgc agcgacaaag cctatgcgtt tcttgacatc gacaatcgcg
      721 gcgccttcga agctgccaca aggctgcttg tggacctcgg gcaccgtcgc attgccttga
      781 tcaacggtga cccgcgctac aacttcgcag cggaccggga gaccgggttc cgcgacgctc
      841 tgcgcgcacg cgggatcgcg cctgccccgg ccctgatcag cggcggccca atgcatgaag
      901 gtaccgggca tgcacaggcc cttcgcctgc tcgacagcga ggacccgccg acggcgttcc
      961 tttgttccag cctcgcccag gcggcaggcg tgcgacgggc ctgcctggac cgtggcctgc
     1021 gcgccgggca ggatttggcc ctgatcgcgc acgatgaccg gcttcaggaa atgcgcgcag
     1081 aagctttcga tccgcccctg accacgactc aatcacccat cggcgatgct ggccgccgca
     1141 tcgtcgaact cctggttgct ttgctgcgcg accccaaaca ggtcttgccg gcagaagtct
     1201 ggccggtcga tctcgtcgtc cgcaactcca ctccagcgcc gaagccataa gctcaacgtt
     1261 cgcgccagct atgggcgatc tggtcgaaga gcggcttcac catgtaggac aagggcgttc
     1321 tagccccggt ctggataaac gcctcggccg gcattccagg acgaagacgg ttgtcgccaa
     1381 ggagcgccag ttcggtggcc ggaatcttga gctgaaccat gtagaacggc ggagaattgg
     1441 catcgacacg cgacacgtcg gctgcgacat gggtcacctc ggctccgatt tcgggtgtga
     1501 cgcgggtgtc gaatgccgaa aatctcaccc gtgccgtctg tcccagcgtc acgcgatcga
     1561 tctgcgaagg cggaacctgc gcctgcaata caagatcatc actctcgggg acgatcagca
     1621 tcagggtctc ggccggcgtg atgaccccac caaccgtatg caccgacagc tgatagacgg
     1681 cgcctgaaat gggggccctg atggtcgtgc gttccagccg tgttttgatg gagacgcgac
     1741 gttcgttcag ttccgcgatt cgtgcctcgg tctcgcgcag ctgcgccagg gtctcgctca
     1801 gagtctcctc tcggatctgg aggatctgga gcttcacctc gcccacgcgg gactctgcgc
     1861 tggccttggt agcaatcagc tcgccgcgtt cgccttccag ccttgcgcgc tcgcgctgca
     1921 tggcaaggac gcgcgtgttc ggaacaaggc ccttggcctg tagatccagg agaccgacga
     1981 gctcctcccc gatcagcttg atctggcgtt cctttgcatc cgcctgggcc aggatacctt
     2041 ggacctgttc gtcaatctgc ccgatctgct gcttcagttg ctcgaccttg ccctgaatcg
     2101 tggcgcgctt ggcggcaaag agcttttgct ggccgatcat cagcttggcg atgtcgggat
     2161 cattctctcg gctcaccagc tgctcgggga atacaacttc agcggcttcg tcgcgttccg
     2221 cttcgagacg ggcgcgcttg gccagttctt ccgagagcac ggaatcaatg atgccaagct
     2281 ccgccacatt gtcggtgctg tcgaggacaa caagatcctg accggcctcg acgtgatcgc
     2341 catccttgac caggatggca tcgacgatgc cgccatcgcg gtgctggatg cgcttggtat
     2401 tggattcgac cacgatcgtc gccggtgcga tgaccgcgcc gtggatgctg gcaaaggccg
     2461 accatccgcc gagcactccc acggccagaa acaccgacac atatccggca atctgaaacc
     2521 gacgcagcga cttggcggtg ttgagtgatt tcatgtttca cgtccttccg gtctggacca
     2581 gggtcggacc ggtctccgcg ctgcttttgg ccggctcctt caccaacacc ttccgcaaga
     2641 cttcctcgcg cgggcccagg gcggccatct tgccatcggc aagcacgagc atcttgggga
     2701 tggcggccag cgcactcggg cgatgggcaa ccacgacaac cgtggcacct ctcgccatgg
     2761 catgcctgat cgcgccatcg agcgccgcct ctccctcggc atcgagagcg gaattgggct
     2821 cgtcgaggac aaagactgcg ggatcgccat agagggcgcg ggcaagggcc agacgttggc
     2881 gctggccggt cgacaacctc gacccgttct cgccaagctg ggtgttgtag ccttccggga
     2941 gccgcatgat catgtcgtgc acgttcgcgc gcttggctgc ttcaatgatc ttgccggaat
     3001 ccggctgggg gtcgaagcgg gagatattct gtgctgcagt accatcgaag atctgcaggt
     3061 cctgcggcag atagccgatg gcgcggccaa gcgcgtcggg ctcgcgctga tcgagcgtcg
     3121 cgccatcgag acggatgtgg ccgcgtgttg gctgcagaac ccccacgagg gcgcgcgcga
     3181 gtgtggactt cccggccccc gtgggtccga tgacgccaag accctcgcca ggctgaaggc
     3241 tgaagctgat gccatgcagc aatggcttct cgacacccgg aagctggatg atgaggtttt
     3301 cgacatcgag cttgcccctt ggtggcggca ggaccatcgg cggcgcttcc gccggaattg
     3361 acgagaggac ggccgagaga cgctcacgcg ccttgcggta ggagatgaac tgctgccagc
     3421 tacccacggc ctgttcgacc ggcgcgagcg cgcgcgacat gatgatggat gcggcaatca
     3481 tggcgccgcc ggtgatctcg tgctggatgg cgagataggc accgagcccg aggatgccgg
     3541 actgtaccat gaggcgcaga actcgtgagg ccgcagacaa gatgccgcca gcatcgtttg
     3601 cgaccgtctg ctgatccagc gccgcacttt gcacgccggt ccagcgctcg cgcaggttcc
     3661 ggagcatgcc aagagaatgc agtgcctcgc tgttgcgacg gctttcttcg gaaatctggg
     3721 ccgccttgat gctggacttg gtcgcctcgg caatggggcc acgggtcacg cgctcggaga
     3781 tcagcgcgag ggtgaagatg gccacggcgg cggccacggc ggccacaccc aggctccagt
     3841 gaatgagata gatgatcaga aggtagatcg gcatccaggg catgtcgaag aaggcgaaag
     3901 gcccttgccc cgaaagatac tgccggattg tgccgaggtc gttgaccggc tgcgccccga
     3961 tgcctggcgt acgtcgcaag gcatgcgccg tcaccacatc gaagacccgg ccgcgcagtt
     4021 gttcctccac gcgtcgcccg atcctcacca ggatgcgcga tctcagatat tcgaggaacc
     4081 cgtagtaggc atagagcccg atgacgatga tgctgagtgc aagcagcgtc ggcacgctgc
     4141 ccgacgcgag gacccggtca tagacctgca tcatgtacag gggaccagtc agcatcagga
     4201 tgttgacaag acagctgaag atgccagtgg aaatgaaagc cccgcggctg gcgcggtata
     4261 cggctttcag ctcttgggct tcgggacttc caggtggcgt ctgcggcacg tgccgtctcc
     4321 agtgttatgg aaaccaagct accgacacaa acatacgatc aggttaacga ctggtttaaa
     4381 cctgcctcaa gccaaggtgc cgttcccctg gctcacggta aagtcgcggc gggccgcaac
     4441 gatcgagacc gagaagccgg cgaccacgtc gatcaaggtc atgaccatga gcagaaagaa
     4501 cgtgaccgtc ccgcaagacg gcaccaggag gaaggcgatc aatgcgccga tgaatgctgc
     4561 ggtcgacagc atgtggtcga gaatggtgtt cccggcgtaa cgggccgcct tgagaatttc
     4621 gatgaaaagc gccaccaggc ctgccatcac gaagacatct ccgaccagga agaatatctc
     4681 cgcgcccgag ggcagtacgg tgtcgtagaa ggtcgagtcg ggctgcagca tgctgccgct
     4741 ctgggcgagc agcacatagg ccaggatgac cacggccagg agggggatcg agagaatgaa
     4801 gcgcatggac gtctccagag gtat
//
LOCUS       DBNGVR010000282         2989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4424812, whole genome shotgun sequence.
ACCESSION   DBNGVR010000282 DBNGVR010000000
VERSION     DBNGVR010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4424812"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC16A0_06045"
     CDS             complement(<1..632)
                     /locus_tag="AC16A0_06045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3104927.1"
                     /translation="MKLGLKGRRALVTGGTRGIGRAIAFALAAEGVDVGICARDAAAV
                     TATVAELKATGVRAAGRALDVADATALKAWVTETAQGFGGLDILVANTSAMAEGATET
                     EFRKAFEVDVLHTLNAVTAAMPFLETSRGGSIVAISSISGSEDYGYEGVSYGAMKAAL
                     LFYMKSLARHVAPKGIRANVVSPGTTYFAGGFWHEVEIRDPKGFAATIAE"
     gene            complement(634..1458)
                     /locus_tag="AC16A0_06050"
     CDS             complement(634..1458)
                     /locus_tag="AC16A0_06050"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HIR3104928.1"
                     /translation="MADISSFAATFAAAPGKAFKLSSRDPRDQSAFADKNKAKAQTAE
                     DAAAIDALQDRLFADGRKALLVVLQGIDCSGKDGTVRAVFNSCGPIGVKVHPFKAPTP
                     PELAHDYLWRVHKACPERGMIGIFNRSHYEDVLVVKVRKFASPDAIERRYEEINQFEK
                     MLTDNGTVILKFMLHISKQEQAARLQERIDNPDKRWKFNPDDLEDRALWDDYMAAYET
                     AIERCSTPHAPWHVIPADRNWVRNAAVARIVRETLEKIDPQYPAPKGWDPKTVKVV"
     gene            1589..2314
                     /locus_tag="AC16A0_06055"
     CDS             1589..2314
                     /locus_tag="AC16A0_06055"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3104929.1"
                     /translation="MAAPAYRLPAMDMDFLLGDTTRGLRFQLEYQKAEEHLRRWGVAT
                     TVVVFGSARVSEGDGSGWYEAARELARIVSLEGGARHAHHAMRSNVIATGGGPGIMEA
                     ANRGALDAGAPSIGFNITLPMEQEPNAYSTPDLTFRFHYFAMRKMHFAMRASALVVFP
                     GGFGTMDELFEILTLRQTGKMDDALPVVLYDSAFWKKTINFQHLAETRMINGADLDLF
                     GFADSPQEAWAELLRRGLAVPKE"
     gene            2318..>2989
                     /locus_tag="AC16A0_06060"
     CDS             2318..>2989
                     /locus_tag="AC16A0_06060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3104930.1"
                     /translation="MSIRLRFHGAARTVTGSCYLLEAGSQQLLIDCGMFQGSKTEREL
                     NYRPFPFDAGRVNAMILTHAHIDHSGLIPKLVKAGFGGRIHCTPPTVDLCSIMLPDSG
                     YIQEMEVRQLNERRRKRGEEEVDPIYTLEDATRALESFTNAAYEMWFEPVPGIRARFW
                     NAGHLLGSASVEVEVARDGGKPLRLLFSGDIGPDNKMLEQDPEAPAGFDVVIWESTYG
                     GRDRIE"
BASE COUNT          501 a          996 c          945 g          547 t
ORIGIN      
        1 ttctccgcga tggttgcggc gaagcctttc ggatccctga tctccacctc gtgccagaag
       61 ccgccggcga agtaggtggt accaggcgag acgacattgg cccggattcc tttcggcgca
      121 acatgccggg ccagcgactt catgtagaac agcagcgccg ctttcatggc gccgtaggac
      181 acaccctcgt agccatagtc ctccgatcca gagatggagg agattgcgac gatcgagccg
      241 cccctggatg tttcgaggaa gggcatcgcc gccgtcacgg cgttcagcgt gtgcagaacg
      301 tccacctcga aggccttgcg gaactctgtt tctgtcgcgc cctccgccat ggcgctggta
      361 ttggccacca ggatgtcgag gccgccgaag ccttgggccg tctccgtcac ccaggccttg
      421 agtgccgtgg catcggccac atccagcgcg cggccagctg cgcgaacgcc cgttgccttg
      481 agctccgcca cggtcgccgt cacggccgcg gcgtcacgcg cgcagatgcc gacatcgacg
      541 ccttccgccg ccagcgcgaa ggcgatggcc cggccgatgc cgcgcgtgcc gccggtgacc
      601 agcgcacgcc tgcccttcaa tccaagtttc atgtcagacc accttaaccg tcttgggatc
      661 ccagcccttg ggtgcggggt actgcggatc gattttctcc agcgtctcgc gcacgatgcg
      721 ggcgacggcg gcattgcgta cccagttgcg gtccgccggt atgacatgcc acggtgcgtg
      781 aggggtcgag cagcgttcga tggcggtctc gtaggccgcc atgtagtcgt cccacagggc
      841 gcggtcctca aggtcgtccg gattgaactt ccagcgcttg tcgggattgt cgattcgctc
      901 ctgcagccgc gcggcctgtt cctgcttcga gatgtgtagc atgaacttca ggatgaccgt
      961 gccattgtcg gtcagcatct tctcgaactg gttgatctcc tcgtagcggc gctcgatcgc
     1021 gtccgggctg gcgaatttgc ggacctttac caccagcacg tcttcgtagt gcgagcggtt
     1081 gaagatgccg atcatgccgc gctcggggca ggccttgtgc acgcgccaca gatagtcatg
     1141 cgcgagttcg ggcggcgtcg gcgccttgaa gggatggacc ttcacgccga tcggtccgca
     1201 actgttgaag acggcgcgta ccgttccatc cttgccggag cagtcgatgc cctgcagcac
     1261 caccagcagg gctttccggc cgtcggcaaa gaggcggtcc tgaagtgcat cgatggcggc
     1321 cgcgtcctcg gccgtctggg ccttggcctt gttcttgtcg gcaaaggccg actgatcgcg
     1381 cgggtcccgg ctggagagct tgaacgcctt tcccggcgct gcggcaaaag tggcggcaaa
     1441 ggaggagata tcggccattc atgaagtccc gaactgtggc tgcggcccaa gcttagccct
     1501 cttgattgcg gcttgtcatg gccctatccc ggagaccccg ccctgcaagg agaacccacg
     1561 catcgtgacc gcatcgaaca aggcacagat ggccgcgccg gcctaccggc tgccggccat
     1621 ggatatggat ttcctgcttg gcgacaccac ccgcggcctg cgcttccagc tcgagtacca
     1681 gaaggccgag gaacatctgc ggcgctgggg cgtggccacc acggtggtgg tcttcggctc
     1741 cgcccgtgtc agcgaaggcg atggctcggg ctggtacgag gcggcgcgcg aactggcgcg
     1801 catcgtgtcg ctcgagggcg gcgccaggca cgcccatcat gccatgcgga gcaacgtgat
     1861 cgccaccggc ggcgggccgg gcatcatgga ggccgccaac cgcggcgccc tcgatgccgg
     1921 ggcgccgtcc atcggtttca acatcacgtt gccgatggaa caggaaccca atgcctattc
     1981 cacccccgat ctcaccttcc gcttccacta cttcgccatg cgcaagatgc acttcgccat
     2041 gcgggcatcg gccctggtgg ttttccccgg cggcttcggc accatggacg agctcttcga
     2101 gatcctgacg ctgcgccaga ccggaaagat ggacgacgcg ttgccggtgg tgctctatga
     2161 ttccgccttc tggaagaaga ccatcaactt ccagcatctt gccgagacgc gcatgatcaa
     2221 cggggccgac ctcgatctct tcggctttgc cgacagcccg caggaggcct gggccgagct
     2281 gctgcgccgc ggcctcgctg tgcccaagga gtaagccatg tccatccgcc tgcgcttcca
     2341 tggtgccgcc cgcactgtca cgggctcctg ctacctgctc gaggccggca gccagcagct
     2401 gctgatcgac tgcggcatgt tccagggatc gaagaccgag cgcgaactca actaccggcc
     2461 cttccccttc gacgcaggcc gggtcaacgc catgatcctc acgcacgcgc acatcgatca
     2521 ttcgggcctc attccgaaac tggtaaaggc cggcttcggc ggcaggatcc actgcacgcc
     2581 gccgaccgtt gacctctgca gcatcatgct gcccgattcc ggatacatcc aggaaatgga
     2641 agtgcggcag ctcaacgagc ggcgccgcaa gcgcggcgag gaagaggtcg accccatcta
     2701 tacgctcgag gatgccacca gggcgctcga gagctttacc aatgcggcct atgaaatgtg
     2761 gttcgaaccg gtgccgggca tccgggcacg gttctggaac gccgggcatc ttctgggatc
     2821 cgcctcggtg gaggtcgagg tggcgcgcga cggcggcaag cccctgcgcc ttcttttctc
     2881 cggcgacatc ggccccgaca acaagatgct ggagcaggac ccggaagcgc ccgcgggctt
     2941 cgacgtcgtg atctgggaat cgacctatgg cggccgcgac cgcatcgag
//
LOCUS       DBNGVR010000283         3302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2460178, whole genome shotgun sequence.
ACCESSION   DBNGVR010000283 DBNGVR010000000
VERSION     DBNGVR010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2460178"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="AC16A0_06065"
     CDS             <1..937
                     /locus_tag="AC16A0_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104931.1"
                     /translation="VPLIVLVMSVAIFGLLVGTKFFNPFSLTLILQQVAIVGIVGAAQ
                     TLVILTAGIDLSVGAVMVLSSVVMGQFTFRYGIPVPISILCGLVTGTLCGFINGWLVA
                     RMKLPPFIVTLGTWQIFNAIKYLYSANETIRTQDIEAQAPLLQLFGTQLKLGGAVFTL
                     GVIFMVLLVVFLWYVLNYTAWGRRLYAVGDDPEAAALSGIRVDRMLITTYTLAGFICG
                     IAGWVLIGRIGSVSPTAGEFANIESITAVVIGGISLFGGRGSILGMIFGACIVGVFSL
                     GLRLLGTDPQWTYLLIGVLIIGAVTVDQWIRKVSG"
     gene            937..1719
                     /locus_tag="AC16A0_06070"
     CDS             937..1719
                     /locus_tag="AC16A0_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3104932.1"
                     /translation="MAQEPILTARRLVKRYGRVTALDNADFDLYPNEILAVIGDNGAG
                     KSTLIKAICGAVQPDEGEIKLEGQTLNFKSPMDARQAGIETVYQNLALSPALSISDNM
                     FLGREIRKEGVLGSWFRMLDRNTMERRAREKLTELGLMTIQNISQAVETLSGGQRQGV
                     AVARAAAFGSKVVIMDEPTAALGVKESRRVLELILDVKRRGLPIVLISHNMPHVFEVA
                     DRIHIHRLGRRLCTINPKDFSMSDAVAFMTGAKEPPAELKAA"
     gene            complement(1730..>3302)
                     /gene="ggt"
                     /locus_tag="AC16A0_06075"
     CDS             complement(1730..>3302)
                     /gene="ggt"
                     /locus_tag="AC16A0_06075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3104933.1"
                     /translation="VGYALAVVNPCCGNIGGGGFATLHLADGRDVFLNFREKAPLAAT
                     EAMYLDDKGEVIKDLSLRGYLAVAVPGTVLGLDTLLRDYGTLPRDVVMAPAIRLAEEG
                     FILDQGDVDILSRAADSLVAEPNVASIFLKDGKPLSAGDRLVQSNLAATLRSIAEQGP
                     DAFYRGSIADRIVAASSTNGGILSKKDFESYSVSEEKPVRCSYRSYEIISAPPPSSGG
                     TAICQILNVLEGYSIAEFGWHSAQSLHVMAEAMRHAFVDRNFLLGDPAFVSNPLDQLL
                     SEAYAADIRSRIDLGKAAKSEEVRPGTPPHGGTETTHYSVVDAQGNAVSVTYTINALF
                     GAKVIAGDTGFFLNDEMDDFTIKPGVANLFGLVQGKTNAIAPGKRPLSSMSPTIVTKD
                     GKTFMVLGSPGGSRIITIVVQVILNVIEHGMTIQEAVNAPRMHHQWLPDQMAVEPFAL
                     SPDTREKLEAMGHKVVEQSEWGAAEAILIGPAKVATDATASSGNDAMAKRKQVPGLLY
                     GGHDYRRPSGGVAGH"
BASE COUNT          583 a         1009 c         1036 g          674 t
ORIGIN      
        1 cgttccgctg attgtgctcg tcatgtcggt ggcgatcttc ggcttgctgg tcggcaccaa
       61 gttcttcaat cccttctcgc taaccctgat cctccagcag gtggccatcg tcggcatcgt
      121 cggggccgcg cagacgctgg tcatcctgac cgctggcatc gatctctcgg ttggcgcggt
      181 catggtgctg tcctctgtcg tgatggggca gtttaccttc cggtacggta tacctgtgcc
      241 gatctccatt ctctgcggcc tggtgacggg cacgctctgt ggtttcatca atggctggct
      301 ggtcgcccgc atgaagctgc cgccgttcat cgtgacgctc ggcacctggc agatcttcaa
      361 tgcaatcaag tatctctact ccgccaatga aacgatccgt actcaggaca ttgaagcgca
      421 agcgccgttg ctgcagctct tcggcacgca gttgaaactg ggcggtgctg tattcaccct
      481 gggcgtgatc tttatggtgc ttctggttgt cttcctctgg tacgtgctga actatacggc
      541 gtggggtcga agactctatg ccgttggtga cgatccggaa gcggcagcac tgtcgggtat
      601 ccgcgtcgac cgcatgttga tcaccaccta cacgctggcg gggttcatct gcggcatcgc
      661 gggttgggtg ctcatcggcc gcatcggctc ggtttcgccg accgcgggcg aattcgccaa
      721 catcgagtcc atcacggccg tggtgatcgg cggcatttcg ctcttcggcg ggcgcggatc
      781 cattctcggc atgattttcg gcgcatgtat cgtgggcgtg ttctcgctgg gcctgcgcct
      841 gctcggtaca gacccgcagt ggacctatct gctcatcggc gtgctcatca tcggcgccgt
      901 cacggtcgac caatggatca gaaaggtatc aggctaatgg cacaggaacc gatcctcacc
      961 gcgcgcaggc tggtcaagcg ctatggccgc gtgacggccc tcgacaacgc ggatttcgat
     1021 ctctacccga acgaaatcct ggctgtcatc ggcgacaatg gtgcgggcaa gtcgacgctg
     1081 atcaaggcca tctgcggcgc cgtgcagccg gacgagggcg agatcaagct cgaaggccag
     1141 accctgaact tcaagtcgcc catggatgcg cgtcaggcgg gcatcgagac ggtttaccag
     1201 aatctcgcgc tctcgcctgc gctttccatc tcggacaaca tgttcctggg ccgcgagatc
     1261 cgcaaggagg gtgtgctcgg atcatggttc cgcatgctcg accgcaatac gatggagagg
     1321 cgcgcgcgag agaagctcac ggagctgggg ctgatgacca tccagaacat ctcgcaggcc
     1381 gtggaaacac tgtcaggcgg tcagcgccag ggcgttgccg tggcgcgcgc cgcggcattc
     1441 ggatccaagg ttgtcatcat ggatgaaccg actgccgctc ttggcgtgaa ggagtcgcgc
     1501 cgcgtgcttg agctcatcct cgacgtcaag cggcgcggac ttccgatcgt gctgatctcg
     1561 cacaacatgc cgcatgtctt cgaagtggcc gaccggatcc acatccaccg cctcggccgc
     1621 cgcctctgta ccatcaatcc gaaggatttc agcatgtcgg acgctgtcgc cttcatgacg
     1681 ggcgcgaagg aaccgccggc agagttgaag gctgcctagc cggccaagat caatggccgg
     1741 cgacgccgcc tgacggccgc cgataatcat gaccgccgta gaggaggccg ggaacttgtt
     1801 tccgcttggc cattgcatca ttgccggagc tcgctgttgc atcggttgcc accttggccg
     1861 gaccgatgag aatggcttcc gcggcgcccc attcggactg ctccaccacc ttgtgaccca
     1921 tggcctcgag cttctcccgc gtgtccggcg atagggcaaa gggctcgact gccatctggt
     1981 ccggcagcca ctgatgatgc atgcgtggcg cattcacggc ttcctggata gtcatgccat
     2041 gttcgatcac gttcaggata acctgaacca cgatggtgat gatgcgcgaa ccgcccggtg
     2101 acccaagaac catgaaggtc ttgccatctt tggtcacgat cgtcggactc atggaactca
     2161 ggggccgctt gccgggcgcg atggcgttgg tcttgccctg caccagtccg aagaggtttg
     2221 ccacaccggg cttgatggtg aaatcatcca tctcgtcgtt gaggaagaag ccggtatcgc
     2281 ctgcaatgac ctttgcgccg aaaagggcat tgatcgtgta ggtgacggaa acggcgtttc
     2341 cctgcgcgtc gacaacggaa tagtgcgtcg tctccgtgcc gccatgcggt ggtgtgccgg
     2401 gccggacctc ttccgatttc gccgccttgc cgaggtcaat tctcgaccgg atgtccgcgg
     2461 cataggcctc tgacagcagt tgatcgagcg ggttgctgac gaaggccggg tcgccgagca
     2521 gaaagttccg gtcgacgaaa gcgtggcgca tcgcttctgc catcacatga agtgactgcg
     2581 cggaatgcca gccgaactct gcgatggaat agccttcgag aacgttcagg atctggcaga
     2641 tggccgttcc gccggagctt ggcggcggcg ccgagataat ctcatagctg cgatagctgc
     2701 agcgcaccgg cttctcctct gagaccgaat agctctcgaa gtccttcttc gagagaatgc
     2761 cgccattggt gctgctggcc gccacgatcc ggtccgcaat ggacccccga tagaaggcgt
     2821 cggggccctg ttcggcgatc gacctgaggg tggcggcgag gttcgactgg accagccgat
     2881 cgccagccga cagcggcttg ccgtctttca ggaagatcga ggccacgttc ggttcggcga
     2941 cgagtgagtc cgccgcgcgg gacaggatgt cgacatctcc ctgatcgagg atgaagccct
     3001 cttcggccag acggatggcc ggcgccatga ccacgtcgcg cggaagcgtg ccatagtccc
     3061 tgaggagggt gtcgaggccg aggacggtac cgggaaccgc aacggcgagg tagccccgga
     3121 ggctgaggtc cttgatcacc tcgcccttgt cgtcgagata catggcttcg gtcgccgcca
     3181 ggggcgcctt ctcgcggaag ttcaggaaga cgtcccgccc gtcggcgagg tgcagcgtcg
     3241 caaagccgcc gccgccgatg tttccgcaac agggattgac cacggccagg gcataaccca
     3301 cg
//
LOCUS       DBNGVR010000284         6616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2657728, whole genome shotgun sequence.
ACCESSION   DBNGVR010000284 DBNGVR010000000
VERSION     DBNGVR010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2657728"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..991
                     /gene="lipA"
                     /locus_tag="AC16A0_06080"
     CDS             38..991
                     /gene="lipA"
                     /locus_tag="AC16A0_06080"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR3104934.1"
                     /translation="MVTLLDTVKAKARHPEKANRPDTPLLRKPDWIRVKAPGSAVYAE
                     TQKIVRDNKLVTVCEEAGCPNIGECWSKKHATFMIMGDTCTRACAFCNVKTGLPDALV
                     QDEPEKVADAVARMGLSHAVITSVDRDDLDDGGAGHFARTIRAIRAASPTTTIEVLTP
                     DFLKKDGALEQLVAAGPDVFNHNLETVPSLYLTIRPGARYFHSLRLLQRVKELDRDMF
                     TKSGIMVGLGETREQVMQVMDDMRSASIDFITIGQYLQPSRKHAAIDRYVTPDEFKSL
                     ETIAYAKGFLMVASSPLTRSSHHAGDDFARLRAARQRQNGR"
     gene            942..1469
                     /locus_tag="AC16A0_06085"
     CDS             942..1469
                     /locus_tag="AC16A0_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR3104935.1"
                     /translation="MISRGCAQPGSGKTDVDAVPKFNVTRTTPHSAENIFAIVADVGS
                     YQEFLPVMKQSRFMHRKTLPDGRESFDATTTIVHDKLRVHETMHSHVIVDRESSTVTA
                     HSADGPLKSLDTRWVIRPLSNGGSEIEFSVDYALKSRSLQFLLSGMFDLVVRKIMNAF
                     EARADELYGSRSASS"
     gene            complement(1442..1945)
                     /locus_tag="AC16A0_06090"
     CDS             complement(1442..1945)
                     /locus_tag="AC16A0_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR3104936.1"
                     /translation="MTFSPGLLRAAEHLLETCRCKGRLIATAESCTGGLIAGVLTAIP
                     GSSDVVDSGFVTYSNDAKAKMIGVSDATIRQHGAVSAEVAVAMAQGAIAQSRAHLAIA
                     VTGVAGPGGGTPMKPVGLVWLAAAVRDGHVRTRELRLGDIGREEVRLATVSEALTMLQ
                     ELADRDP"
     gene            complement(1942..3111)
                     /locus_tag="AC16A0_06095"
     CDS             complement(1942..3111)
                     /locus_tag="AC16A0_06095"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HIR3104937.1"
                     /translation="MTVAAVIVAGGSGLRAGGERPKQYQVIGGKPVIWWTLKAFLDHP
                     GVTHVQAVIGDGHEEMFAESTMGLAVPPPVTGGGSRQDSCRIGIEALEGHAPDKVLIH
                     DAARPFVSSDLISHVIAWLDRFPAVIPGMPVTETVKYAPGGMIDRTVDRASLWTAQTP
                     QGFGFGDILAAHRAAQASAAANLTDDASVAERAGIALAMVPGRIENRKLTTAEDIEQA
                     NRLMTTKSFAELSDIRMGQGIDIHSFESGDAVTLLGVRIPFHHRLTGHSDADAAMHAL
                     TDAIFGAIGEGDIGVHFPPSDAQWKGADSRIFLEKAVSMLHAKGGVLANADITILAEA
                     PKIAPHIPAMKAVLVPLLHLSPDRIAVKATTTEKLGAIGRGEGIMAFATVTVRLP"
     gene            3221..4243
                     /gene="dusB"
                     /locus_tag="AC16A0_06100"
     CDS             3221..4243
                     /gene="dusB"
                     /locus_tag="AC16A0_06100"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR3104938.1"
                     /translation="MGIRIGPIETRNRVFLAPMSGVTDEPFRSVAHHCGAGLVVTEMV
                     ASEELVNSRPDMVRRATGADAVKPFVVQLAGREARWMAEGARVAQDLGADIIDINMGC
                     PARQVTGGLSGSALMRDVDHATALIEATVGASNVPVTVKMRLGWDHGNLNAPELARRA
                     EDSGAAMVTVHGRTRCQFYGGKADWQAIARVRAAISIPLIANGDGVTPADARSMLSQS
                     GADGIMIGRGAYGRPWWPGVIADCLEPGSGRAEPSLTEEAAIVLHHHAAILAHYGPHH
                     GNRVARKHVGWVIDRLAERAFLAADEVQAWRARLLRSGDNSSVAAGIRDLYDTCISAQ
                     EERAVA"
     gene            4240..5379
                     /locus_tag="AC16A0_06105"
     CDS             4240..5379
                     /locus_tag="AC16A0_06105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3104939.1"
                     /translation="MNEIKLQAPEVPLPRAIIDSLPNPLVIIDETGRICLANAAAENY
                     FHTSSNMLTRLSLRDLVPFSSPVLEAVAQTRHTAGVVNEYAIAVGTPRLGGERTVDLQ
                     AALMTEDPRFVIIMLLERSMAHKIDRQLTSRGAARSVSGMASMLAHEIKNPLAGIRGA
                     AQLLEPSLSSEDRTLARLICDETDRIRDLVDQMEVFSDERPLEKKPVNIHVVLERVKQ
                     LTLAALPSGIVVREDYDPSLPPVLGSKDQLVQVFLNLAKNAAEAIAQGGEAGEIIFTT
                     AFRPGVRLTVPGINERITLPLEVCIHDTGPGVPEELRPNIFDPFVTTKPGGRGLGLAL
                     VAKTVRDHGGIVECVGRERGTTFRVLLPMLRVSAIKPADTEEENS"
     gene            5376..>6616
                     /gene="ntrC"
                     /locus_tag="AC16A0_06110"
     CDS             5376..>6616
                     /gene="ntrC"
                     /locus_tag="AC16A0_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR3104940.1"
                     /translation="MSAKTVLIADDDSAIRTVLTQALSRAGYSVRSTATASALWRWVS
                     EGQGDAVVTDVVLPDENAFDVLPRIKKLRPNLPVIVMSAKNTIMTAITAAERGAYDYL
                     PKPFDLNALVQTVGRALEQSSKPVPANSNAPGEALPIVGRSPAMQEIYRIIARLTQTD
                     LTVMIVGESGTGKELVARALHDYGRRRKGAFVAVNMAAIPRELIESELFGHEKGAFTG
                     ATSRVAGRFEQAEGGTLFLDEIGDMPLEAQTRLLRVLQQGEYTTVGGNQPIKTNVRII
                     AATHRDLRQLINQGQFREDLFYRLNVVPLRLPPLRERSEDIPDLVRHFLSMAVKEGLP
                     RKEIAADAVTRLQQHRWPGNIRELENLLRRLVAIDIDDTVTIQAVERELASTPSQVKL
                     DVSEISTGSLPEFMENYLSRY"
BASE COUNT         1228 a         2137 c         2120 g         1131 t
ORIGIN      
        1 tctatctcta tcggctgatg aagtcgaagc aagcctgatg gtcacgctgc tcgacacggt
       61 gaaggccaag gcgcgccatc cggagaaggc caaccggccc gacacgccgc tgctgcgcaa
      121 gcctgactgg atccgcgtca aggcgcctgg ttcggctgtc tacgccgaga cccagaagat
      181 cgtgcgcgac aacaagctgg tgaccgtctg cgaggaggcg ggctgcccca acatcggcga
      241 gtgctggtcc aagaagcatg cgaccttcat gatcatgggg gatacctgca cgcgggcctg
      301 cgccttctgc aacgtgaaga ccggattgcc cgacgccctg gtccaggatg agccggagaa
      361 ggtggccgat gccgtcgccc gaatgggcct gtcgcatgcg gtgatcacct cggtcgatcg
      421 cgacgacctc gacgatggcg gcgcagggca cttcgcccgc accatccggg ccatccgcgc
      481 tgcctcgccg accaccacca tcgaggtgct gacgccggac ttcctcaaga aggatggcgc
      541 cctcgaacag ttggtcgcgg cgggcccgga cgtgttcaac cacaatctcg agaccgtgcc
      601 gtcgctctac ctgaccatcc ggcccggcgc gcgctacttc cactccctgc gcctgcttca
      661 gcgcgtgaag gaactcgacc gcgacatgtt caccaagtcc ggcatcatgg ttggactcgg
      721 cgagacgcgt gagcaggtga tgcaggtgat ggacgacatg cgttccgcca gcatcgactt
      781 catcaccatc ggtcagtacc tgcaaccgag ccgcaagcac gcggccatcg accgctacgt
      841 gacgccggac gaattcaaga gcctggagac catcgcctat gccaagggct tcctgatggt
      901 ggcgtcctcg cccctgaccc ggtcctcgca tcatgccggc gatgatttcg cgcggctgcg
      961 cgcagcccgg cagcggcaaa acggacgttg acgcggtgcc caagttcaac gtcactcgaa
     1021 cgacgcccca ctctgcagaa aacatattcg caattgtcgc ggatgtcggc tcctaccagg
     1081 agttcctccc ggtcatgaag cagagccggt tcatgcatcg aaagacactg ccggatggtc
     1141 gcgagagctt cgatgcaacc acgaccatcg tgcatgacaa gctgcgggtt cacgaaacca
     1201 tgcacagcca tgtcatcgtg gaccgggaaa gcagcaccgt gacggcgcat tcggcagatg
     1261 ggccgctgaa atccctggat acgcgctggg tgatccggcc tctctccaac ggcggctcgg
     1321 aaatcgagtt cagcgtggac tatgcgctga agagccgaag cctgcagttc cttctgtctg
     1381 gcatgttcga cctggtagtc cgcaagatca tgaacgcctt cgaagcgcgc gccgatgagc
     1441 tctacgggtc gcgatccgcc agctcctgaa gcatcgtcag cgcttcgctg actgtcgcca
     1501 gccggacctc ttcgcggccg atgtcaccca gccgcaactc gcgcgttctg acgtgaccat
     1561 cgcgcaccgc ggcggccagc cacacgagtc cgacgggttt catgggggta ccgccgccgg
     1621 ggcctgcgac ccccgtgacg gcgatggcga gatgtgcacg gctctgcgca atggcgccct
     1681 gagccatggc gacggcgacc tccgcgctca ccgcgccgtg ctgccttatc gtggcatcgg
     1741 aaacgccgat catcttcgcc ttcgcatcgt tcgagtaggt cacgaagccg ctgtcgacca
     1801 catccgaaga cccagggatt gcggtcagaa cgccggcgat cagcccgccc gtgcagcttt
     1861 ccgcggtggc aatgaggcgc cccttgcacc gacaggtctc gagcagatgc tctgcggctc
     1921 tgagcagccc cggtgagaat gtcatggaag ccggaccgtg accgtcgcaa aggccatgat
     1981 gccttcgcca cgtccaatgg cgccgagctt ctcggtggtg gtcgccttca ccgcgatccg
     2041 atccggcgac agatggagga gtggtacaag caccgccttc atcgcgggga tatggggcgc
     2101 gatcttcggc gcttccgcaa ggatggtgat gtcggcattg gcgagtacac cgcctttggc
     2161 gtgcagcatg ctcacggcct tctccaggaa gatgcgtgag tccgcaccct tccactgcgc
     2221 atcgctcggc gggaaatgaa ccccgatgtc gccttcaccg atggcgccga agatcgcgtc
     2281 ggtcagcgca tgcatcgcgg catcggcgtc ggaatggcct gtcaggcggt gatgaaacgg
     2341 gattcggaca cccaacaggg tgacggcgtc tcccgactcg aaactgtgaa tgtcgatgcc
     2401 ctggcccatg cggatgtcgc tcagctccgc aaagctcttg gtcgtcatca ggcggttggc
     2461 ctgctcgatg tcctccgccg tggtgagctt gcggttctcg atgcggcctg ggaccatggc
     2521 aagggcaatg ccggcgcgct cggcaacgga ggcgtcgtcc gtgaggttgg ctgcagcgga
     2581 tgcctgcgct gcacgatggg cggcgagaat gtcgccaaat ccgaaacctt gaggggtctg
     2641 ggccgtccac aggctggcgc ggtcgacggt gcggtcaatc atgccgccgg gcgcgtactt
     2701 cacggtctcg gtgacgggca tgccggggat caccgctgga aagcggtcga gccacgcaat
     2761 cacatgggag atcagatcgg acgacacaaa gggacgcgcg gcgtcgtgga tgagaacctt
     2821 gtcaggtgcg tgtccttcga gggcctcgat gccgatccgg caggagtcct gccggcttcc
     2881 gccgccagtg actggtgggg ggaccgcgag acccatagtc gattcggcga acatctcctc
     2941 gtgaccatcc ccgatgacag cctggacatg ggtcacgccg gggtggtcga ggaatgcctt
     3001 gagggtccac cagatgaccg gcttgccacc gatcacctgg tattgtttcg gtctttctcc
     3061 gcctgcgcgc aggccggaac cgcctgccac aattaccgcc gccaccgtca tgatactgct
     3121 gtagcaacct catacttggc ctgcaagaaa ggcccttgac gcggtgcagc atcggtctat
     3181 cctgccaagg aattagacag ctgactggat tgctcaaata atgggcatac gtataggccc
     3241 catcgaaacg cgaaaccgcg tcttcctcgc gcccatgtcg ggtgtgaccg acgagccatt
     3301 ccgcagcgtg gcgcatcatt gcggcgccgg gcttgtcgtc accgaaatgg tggcgagcga
     3361 ggaactggtg aacagccggc ctgacatggt gcggcgggcg acgggcgcgg atgcagtaaa
     3421 gcccttcgtc gtacaacttg ccggccgcga agcccggtgg atggcggagg gtgcgcgggt
     3481 ggcgcaggat ctcggtgccg acatcatcga catcaacatg ggctgcccag cccgccaggt
     3541 caccggcggt ctctccggtt ccgccctgat gcgtgatgtc gaccatgcga cggcgcttat
     3601 cgaagccaca gtcggtgcca gcaacgtacc cgtgacggtg aagatgcggc tgggatggga
     3661 tcacggcaac ctcaacgcgc cggaactggc gcggcgcgcg gaagactccg gcgcggccat
     3721 ggttaccgtt catggccgta cacgctgcca gttctacggc ggcaaggccg actggcaggc
     3781 catcgcgcgc gttcgcgcgg cgatctccat cccgctcatt gccaatggcg atggcgtgac
     3841 gccagccgat gcacgatcca tgctttcgca gtccggtgcg gacggcatca tgattggccg
     3901 cggtgcctat gggcggccct ggtggcccgg cgtgatcgcc gactgcctcg aacccggatc
     3961 cggaagggca gagccctcac tgaccgagga ggccgcaatc gtgctgcatc atcacgcggc
     4021 catcctggcg cattacggcc cccatcacgg caaccgtgtg gcccgcaaac acgtcggatg
     4081 ggtgatcgac aggctcgcgg aacgcgcatt cctcgcggcg gacgaggtcc aggcctggcg
     4141 cgcacgcctg ttgcgaagcg gagacaacag cagcgtcgcc gccgggatcc gtgatctcta
     4201 cgacacatgc atctcggcac aagaagaacg ggccgtcgca tgaacgagat caagcttcag
     4261 gcgccggagg tgccgctgcc gcgggcgatc atcgactcgc ttcccaatcc cctggtgatc
     4321 atcgatgaga cggggcgcat ctgcctggcc aacgccgcgg ccgagaacta cttccacaca
     4381 agttccaaca tgctgacccg gctttcgctg cgcgatctcg ttccgttttc cagcccggtg
     4441 ctggaagctg tcgcgcagac ccgccacacc gccggtgtcg tcaacgagta tgcgattgcg
     4501 gtgggaaccc cacgcctcgg cggtgaacgc accgtggacc tgcaggcggc cctgatgacg
     4561 gaagatccgc gctttgtcat catcatgctg ctggagcggt caatggcgca caagatcgac
     4621 cggcagctga cctcgcgcgg tgccgcgcga tccgtgtcgg gaatggcctc gatgctggcg
     4681 cacgagatca agaaccctct ggccgggatc cggggcgcgg ctcaactcct ggaaccatcg
     4741 ctgtccagcg aagatcgaac actggcgcgg ttgatctgcg acgaaaccga ccgcatccgc
     4801 gatctggtgg accagatgga ggtgttctcc gacgaacgcc cgctcgagaa gaagccggtc
     4861 aacatccatg tggtgctcga aagggtaaag cagctgacgc tggccgcgct gccgtcgggc
     4921 atcgtcgtgc gcgaggacta tgacccgtcg ctgccaccgg tgctgggcag caaggaccag
     4981 ctggtccagg tgtttctcaa tctggccaag aatgccgcgg aggccatcgc acagggtggc
     5041 gaggcgggcg agatcatctt cacgacggcg ttccgccccg gcgtgcgcct gaccgtaccc
     5101 ggtatcaacg aacgcatcac cctgccgctg gaagtctgta ttcacgatac cggccccggc
     5161 gtgccagaag aactgcgccc gaacatcttt gatcccttcg tgaccaccaa gcccgggggg
     5221 cgcggattgg gtctggccct ggtggccaag acggtgcgcg atcacggcgg catcgtggag
     5281 tgcgtcggac gcgaacgcgg cacgacgttc cgcgtgctgc tgccgatgct gcgcgtcagt
     5341 gcgatcaagc ctgccgatac cgaggaggaa aactcatgag cgccaagacg gtcctgatcg
     5401 ccgacgacga cagcgccatc cggacggtcc tgacccaggc cttgagccgc gccggttata
     5461 gcgttcgttc gactgcaacc gcttcggcgc tgtggcgctg ggtatcagaa ggacagggcg
     5521 atgcggtggt caccgatgtg gtgcttcccg acgagaacgc cttcgacgtg ctgccgcgca
     5581 tcaagaagct gcgtcccaat cttccggtga tcgtgatgag cgccaagaac accatcatga
     5641 cggccatcac ggcggccgag cggggtgcct acgactacct gccaaagccg ttcgatctca
     5701 atgcgctggt gcagaccgtc gggagggccc tcgaacagtc gtcaaagccg gtgccggcca
     5761 acagcaacgc gccgggcgag gcgctgccca tcgtcggccg ctcgcccgcg atgcaggaaa
     5821 tctaccggat catcgcccgc ctgacgcaaa ccgatctcac cgtgatgatc gtgggagagt
     5881 cgggtacggg caaggagctt gtggcgcggg cgctgcatga ctacggccgc cgccgcaagg
     5941 gggccttcgt ggccgtcaac atggcggcca ttccgcgcga actgatcgaa tcggaactgt
     6001 tcgggcatga gaagggcgcc ttcaccggcg caacgtctcg ggtggccggc cgtttcgagc
     6061 aggccgaagg cggcacgctg tttctcgacg agatcggcga catgccgctc gaggctcaga
     6121 cacggctgct gcgcgtgctg cagcaaggcg agtacacgac cgtcggcggc aaccagccga
     6181 tcaagaccaa tgtccggatc atcgcggcga cgcatcggga cctgcgccag ctgatcaacc
     6241 agggacagtt ccgcgaggat ctcttctacc gcctcaacgt ggtgccgctc cgtcttccgc
     6301 cgctgcgcga gcgcagcgaa gacattcccg acctcgttcg gcatttcctg tccatggccg
     6361 tgaaggaagg gctgccgcgc aaggagatcg cggcggacgc tgtgacgcgc ttgcagcagc
     6421 accggtggcc tggcaacatc cgggaactgg aaaacctgct gcgccggctg gtagccatcg
     6481 acatcgacga cacagttacc atccaagccg tagagcgcga actggccagc acgccatccc
     6541 aggtgaagct tgatgtctcg gagatctcta ctggcagctt gccggaattc atggagaact
     6601 atctgtcgcg ctactt
//
LOCUS       DBNGVR010000285         5030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5407679, whole genome shotgun sequence.
ACCESSION   DBNGVR010000285 DBNGVR010000000
VERSION     DBNGVR010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5407679"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="tilS"
                     /locus_tag="AC16A0_06115"
     CDS             complement(<1..518)
                     /gene="tilS"
                     /locus_tag="AC16A0_06115"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3104941.1"
                     /translation="MALLRLAAQAAACRLTVLTVDHGLRPGSAAEARQVGQWCCGLGL
                     AHQTLNWTGASPGSGVQARARKARYDLMADWCNANAASALLTAHSRDDQAETVLMRLL
                     RTDSFDSLAGIPQFGQWHGLPLIRPLLSCRRADLRTYLTALGQDWIEDPSNDDTRFER
                     VRLRRLMPALEET"
     gene            complement(592..1542)
                     /gene="ybgF"
                     /locus_tag="AC16A0_06120"
     CDS             complement(592..1542)
                     /gene="ybgF"
                     /locus_tag="AC16A0_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR3104942.1"
                     /translation="MKKFLRSAAAVALLGLGLITPALAQSASEMAVRMQQLEEQVRML
                     MGQVEELNYEVKQLRAQAGGNQQGAAQPMQPPAQPKQVAEAPAEGVAVETLPPVAGVQ
                     PLEAPDSLNNTEALIVQPDSGAAPGPTILGGIDNSAAKAKDGGFEGVVLVPPSQQEPG
                     DDAFVTQGLNQGAGETAQDSIETVALSPESPEAIYEKANESLLRRQFAEAEAGFGSFM
                     AKYPDHSLAGSAQYWLGETFYAQSDFKRAAQAFLRGYKTYPKSRRAADSLLKLGISLN
                     RLGQTEQACAAYLAVSAEYPKAVEARKRAQAEAKRANCQA"
     gene            complement(1663..2151)
                     /gene="pal"
                     /locus_tag="AC16A0_06125"
     CDS             complement(1663..2151)
                     /gene="pal"
                     /locus_tag="AC16A0_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR3104943.1"
                     /translation="MMSRRASFKLAAAACCFLALAACSKKNTPNLEGGAGGAYPPGSE
                     GEFTTVVGDRIFFSEDQSTLSSEAQETLRRQADWLKKYRNVTVQVEGHADERGTREYN
                     ISLSARRATAAREFLIAQGVTGSRISSIAYGKERPVALCDAEQCWSQNRRAVTVITGG
                     AK"
     gene            complement(2283..2771)
                     /gene="pal"
                     /locus_tag="AC16A0_06130"
     CDS             complement(2283..2771)
                     /gene="pal"
                     /locus_tag="AC16A0_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR3104944.1"
                     /translation="MTTSISFKIAAAFCCLLALSACSKKSTPNLEANAGQAVPGSEQD
                     FAVNVGDRVFFAEDQSTLSPEAQETLRRQAAWLAQYPNVMVQVEGHADERGTREYNIS
                     LSARRATAAREFLIAQGVAAKRVSSIAYGKERPAALCDAEQCWSQNRRAVTVITGGAK
                     TS"
     gene            complement(2866..4224)
                     /gene="tolB"
                     /locus_tag="AC16A0_06135"
     CDS             complement(2866..4224)
                     /gene="tolB"
                     /locus_tag="AC16A0_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR3104945.1"
                     /translation="MTGTRSTMTLSSHAAHSTRRAILAFAVMFAVVLGLKTALAQTEI
                     DITGGRVDPLPIAIAPFLVGGGAEEAGTTINGVITNNLGRSGYFAPIDPASFIEQISD
                     FNTPPRFGDWRQINAKALVTGQVFLEGGKVRAEFRLWDVNTQQQLAAQQFTTTPKNLR
                     RIGHLISDVIYKSLTGIDGYFDTRVVFVQEEGPKSQRVKKLAIMDQDGYNPRPLTDGN
                     ELVLTPRFSPSTNEITYMSFGNDTPRVYLMNIDTRQREIVGEFPNMSFSPRFSPDGQR
                     VIMSLQDGGNSNIFELDLRSRGLRQLTNVPAINTAPSYSPDGSQITFESDRGGTQQIY
                     VMGADGSGQTRISFGNGRYSTPVWSPDGQYIAFTKQGGGKFAIGVMRPDGSGERILTE
                     GFHNEGPTWAPNSRVLMFFRDSPGENGGPQLYSVDITGYNEQQVPTPGFSSDPAWSPK
                     LN"
     gene            complement(4235..>5030)
                     /locus_tag="AC16A0_06140"
     CDS             complement(4235..>5030)
                     /locus_tag="AC16A0_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3104946.1"
                     /translation="QEAVMVDISKISDTTKQMAMTKADEPKPVEKPAPKKAEVVKKPE
                     DTQAKIAEETKTAAKEAKAAEPPPPEPDALKELIKETVAEEPAPKKEKPKKEEVKKAE
                     VKPKEKPKPEKKKPELDVAKLEEILNDDAFLNKENEEKTTDASIAATDGNPAASEANL
                     QGTDNVLSATAIDALRSRIQECWSVPPGAREANITVRVRFQLNTDGTVIGEPQVMNDS
                     AEPLFGTTARSTVAAILGCQSYDFLPQEQYDLWKDLILNFNPNMMS"
BASE COUNT          775 a         1582 c         1697 g          976 t
ORIGIN      
        1 gtctcctcca gcgcgggcat gagccggcgc agcctgacgc gttcgaagcg cgtgtcgtcg
       61 ttgctcggat cctcgatcca gtcctggccg agcgcggtga gatatgtgcg cagatcggcg
      121 cgccgacagg acagcaacgg acggatgagc ggcagcccat gccactggcc aaactgcgga
      181 atgccggcga gactgtcgaa gctgtcggtg cgcagcaggc gcatcagcac ggtttcggcc
      241 tgatcgtcgc ggctatgggc ggtgagcagc gcgcttgccg cattggcgtt gcaccagtcc
      301 gccatgagat cgtagcgggc cttgcgggca cgggcctgga cgccgctgcc ggggctggcg
      361 ccggtccagt tcagggtctg gtgggcaagg ccgaggccgc agcaccactg gcccacctgc
      421 cgggcttcgg cggcagagcc gggcctgagc ccgtgatcga ccgtcagtac cgtcagccgg
      481 caggccgcag cctgcgcagc cagccgcagc agggccattg aatcggatcc gccggagacg
      541 gccagcgcaa catgggaatg tcccgcgaca gacgcggtca gcaggcccgc atcaggcctg
      601 gcaattggca cgcttggctt cggcctgggc gcgcttgcgg gcctcgaccg ccttgggata
      661 ctccgcggag actgccagat aggcggcaca ggcctgttcg gtctggccaa ggcggttcag
      721 cgaaatgccg agcttcagaa ggctgtcggc ggcgcggcgg ctcttgggat aggtcttgta
      781 gccgcgcagg aaagcctggg cggcgcgctt gaaatcgctc tgggcataaa aggtctcgcc
      841 cagccagtac tgggcgctgc cggcgaggct gtgatccgga tacttggcca tgaagctgcc
      901 gaacccggcc tcggcctcgg cgaactgccg gcgcaacagg ctttcattgg ccttttcgta
      961 gatggcttcg ggcgactcgg gcgaaagcgc cacggtctcg atcgaatcct gcgcggtctc
     1021 gccagcgccc tggttcaagc cttgggtgac gaaggcgtca tcgcccggct cctgctgcga
     1081 gggcgggacc agaaccacgc cttcgaagcc gccgtccttc gccttggccg cggaattgtc
     1141 gatgccaccc agaatggtcg gaccaggggc cgcgccggaa tccggctgga cgatcagggc
     1201 ttcggtgttg ttgagcgaat cgggggcttc cagcggctgg acaccggcaa ccggtggcag
     1261 cgtttcgacc gccacgcctt cggccggcgc ctccgcaacc tgcttcggct gggccggcgg
     1321 ctgcatcggc tgggccgcgc cctgctggtt gccgcccgcc tgcgcgcgca actgcttgac
     1381 ctcgtagttg agttcctcga cctggcccat cagcatgcgg acctgctctt cgagctgctg
     1441 catccgcacc gccatttcgg aggccgattg tgccaaggcc ggagtgatca gtccgaggcc
     1501 cagaagggcc actgcagctg ccgatctcag aaacttcttc atgtcagaca ccatagtccc
     1561 ggggccttgc acaggccgtc tcgcatgact tccgactgga acccaatcgc ggtgaaattg
     1621 aggctgtgcg ccgccgccag catcaaagcc aggggcctgt tgctatttcg caccaccggt
     1681 gatgacggtg acggcacggc ggttctgcga ccagcactgt tcggcgtcgc agagcgcgac
     1741 ggggcgttcc ttgccatagg cgatggagga gatgcggctt ccggtgacgc cctgcgcgat
     1801 caggaattcc cgcgccgcgg tggcgcggcg ggccgacagc gagatgttgt attcgcgggt
     1861 gccgcgctcg tcggcatggc cttcgacctg gaccgtgacg ttgcggtact tcttcagcca
     1921 gtcggcctgg cggcggagcg tctcctgggc ttccgatgaa agcgtcgact ggtcttccga
     1981 gaagaatatg cggtcgccca caacggtggt gaattcgccc tcggaacccg gcggataggc
     2041 cccgccggcg ccgccttcga ggttcggcgt attcttcttc gaacaggcgg ccagtgccag
     2101 aaaacagcag gcggcggctg ccaacttgaa gcttgcccta cgcgacatca tgcgaaacca
     2161 ctccttttcc ggacccctct ccggatcatc ctcgtcacgg ccccggcgaa agaggcacga
     2221 acccttgtca cggtctaaag caacagggtt aacattccct cacccgcccg tgccgaacag
     2281 gcctagctcg tcttggcacc gccggtaatg accgtaacgg cgcggcgatt ctgtgaccag
     2341 cactgctcgg cgtcgcagag cgccgccggg cgttccttgc cataggcgat cgaagacacg
     2401 cgctttgccg ccacgccctg ggcgatcagg aactcgcgtg cggccgttgc gcggcgggcc
     2461 gacagcgaga tgttgtactc gcgggtgccg cgctcgtcgg catggccttc gacctgcacc
     2521 atcacgttgg ggtactgggc cagccaggcg gcctggcggc gcagggtttc ctgggcctcg
     2581 ggcgacagcg tcgactggtc ctcggcgaaa aagacccggt caccgacatt gacggcgaag
     2641 tcctgctcgg agcccggcac ggcctggccc gcgtttgcct cgaggttcgg cgtgctcttc
     2701 ttcgagcagg cggacagggc cagaaggcag cagaaggccg cggcgatctt gaagcttatc
     2761 gatgtcgtca tgtcttgatt cccgtgctgt gtggtgttgt cccggtcagc cgcatccccg
     2821 cgccttccgg acttgggccc cccggcccga tggatcgaag ccgcctcaat tcagttttgg
     2881 cgaccatgcg ggatccgacg agaagccggg cgtcggaacc tgctgttcgt tgtagccggt
     2941 gatatccacc gaatagagct gcgggccgcc attctcgccg ggcgaatcgc ggaagaacat
     3001 gagcacgcgg ctgttggggg cccatgtggg cccctcgttg tggaagccct cggtgagaat
     3061 gcgctcgccc gatccgtcgg ggcgcatcac gccgatggcg aacttgccgc cgccctgctt
     3121 ggtgaaggcg atgtactggc cgtccggcga ccacaccggc gtggaatagc ggccgttgcc
     3181 gaagctgatg cgggtctggc ccgatccatc ggcgcccatc acgtagatct gctgggtgcc
     3241 gccgcggtcg gactcgaagg tgatctggct gccatccggc gaatagctgg gcgcggtgtt
     3301 gatggccggc acgttggtca gctggcgcag gccacgcgaa cgcaggtcga gttcgaagat
     3361 gttcgagttg ccgccatcct gaaggctcat gatgacgcgc tggccgtcgg gcgagaagcg
     3421 cggcgagaag ctcatgttgg ggaattcgcc gacgatctcg cgctgccgcg tgtcgatgtt
     3481 catgagatag acccgcggcg tgtcgttgcc gaaggacatg taagtgatct cgttggtgga
     3541 cggcgagaaa cgcggcgtca gcaccagttc attgccatcg gtgagcgggc gcgggttgta
     3601 tccgtcctgg tccatgatgg cgagtttctt gacgcgctgg ctcttggggc cctcttcctg
     3661 cacgaagacg acgcgcgtgt cgaaatagcc gtcgatgccg gtgagcgact tgtagatcac
     3721 gtcggagatc agatgcccga tacggcgcag gttcttcggg gtggtggtga actgctgggc
     3781 cgccagctgc tgctgcgtat tcacgtccca caggcggaac tcggccctga ccttgccgcc
     3841 ctcgagaaag acctggccgg taaccagcgc cttggcattg atctgccgcc agtcgccgaa
     3901 gcgcggcggc gtgttgaaat cgctgatctg ctcgatgaag gaggccgggt cgatcggcgc
     3961 gaagtagccc gagcgcccga gattgttggt gatcacgcca ttgatggtgg tgcccgcctc
     4021 ctcggcgccg cccccgacga ggaacggcgc aatggcgatg ggcagcggat cgacgcgtcc
     4081 gccggtgatg tcgatctccg tctgcgccag cgcggtcttc aggccgagga cgacggcgaa
     4141 catcaccgca aatgcgagaa tggcgcgccg ggtgctgtga gcggcgtggc ttgacagggt
     4201 catggtgctg cgggttccgg tcatctgtcg aacatcatga catcatgttc gggttgaagt
     4261 tcaggatgag gtccttccac aggtcatact gctcctgcgg gagaaaatcg taggactggc
     4321 agccgaggat ggccgccacg gtggagcgcg ccgtggttcc gaacagcggc tcggcggaat
     4381 cattcatcac ctgcggctcg ccgatgacgg tgccgtcggt gttcagctga aagcggaccc
     4441 tgacggtgat attggcctcg cgcgcgccgg gcggcaccga ccagcactcc tggatgcgcg
     4501 accgcagcgc atcgatggcg gtggcggaga gcacattgtc cgtcccctga agattggcct
     4561 cgctggcggc cggattgcca tcggtcgcgg caatgctggc atcggtcgtc ttttcttcgt
     4621 tttccttgtt gaggaaggca tcgtcgttga ggatttcctc aagcttggcg acatcgagct
     4681 ccggcttttt cttctcgggc ttcggctttt ccttgggctt gacctcggcc ttcttgacct
     4741 cttccttctt cggcttttcc ttcttcggcg ccggctcctc ggcgaccgtt tccttgatga
     4801 gttccttcag cgcatcgggc tcgggcgggg gcggttcggc ggccttggct tcctttgctg
     4861 cggtcttggt ctcttcggcg atcttcgcct gggtgtcttc cggcttcttg acgacctcgg
     4921 ccttcttcgg agcgggcttc tcgaccggct tcggttcgtc ggcctttgtc atggccatct
     4981 gcttggtggt gtcgctgatc ttcgagatat cgaccatcac cgcttcctgc
//
LOCUS       DBNGVR010000286         2809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3639808, whole genome shotgun sequence.
ACCESSION   DBNGVR010000286 DBNGVR010000000
VERSION     DBNGVR010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3639808"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="AC16A0_06145"
     CDS             complement(<1..282)
                     /locus_tag="AC16A0_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3104947.1"
                     /translation="MTLIKETDYGTPKSGSPNLVTLEIDGLSVTVPEGTSIMRAAMEL
                     GTKIPKLCATDTLESFGSCRLCLVEIEGRKGTPASCTTPVAHGIKVKTQT"
     gene            complement(294..1838)
                     /locus_tag="AC16A0_06150"
     CDS             complement(294..1838)
                     /locus_tag="AC16A0_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004679305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase beta subunit"
                     /protein_id="HIR3104948.1"
                     /translation="MIRVFGPRESAARSVGADEVAAAIAKHAQHLGLQIVRNGSRGLF
                     WLEPMVEVDTPSGRVAYGPVSAADVDSLVAAKFHEGGTHALSLGNIESHPYLKNQERL
                     TFARCGITDPVSVEDYVKHGGFEGLKKALSMAGAAIVAEVSESGLRGRGGAGFPTGIK
                     WKTVHDAKADQKYVCCNADEGDSGTFADRMVMEGDPLMLIEGMTIAGIATGATRGYVY
                     IRSEYPDAIAAMKEAIAAAVAAKWLGDNIQGTDKSFRLEVRRGAGAYICGEETAMLES
                     LEGKRGMVRPKPPIPALEGLFGKPTVINNVLSFAAVPFILAKGGTVYKDYGMGRSRGT
                     LPFQLAGNIRKGGLVEKAFGITLRELVEGFGSGTFTGRPIRAIQVGGPLGAYLPAEQL
                     DLPMDYEAFAAQGAMVGHGGIVVFDDSVDMARMARFAMEFCAIESCGKCTPCRIGSTR
                     GVETIDKIIAGVDRDANLEVLEDLCQTMTDGSLCAMGGLTPMPVMSAVKHFPEDFDKP
                     ARMAAE"
     gene            complement(1835..2326)
                     /locus_tag="AC16A0_06155"
     CDS             complement(1835..2326)
                     /locus_tag="AC16A0_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR3104949.1"
                     /translation="MSQTYSLIDTSPKEALDARRIALAHGNKPDALIEILHDLQAELG
                     HVPESALPALAKALNLSRAEVHGVVTFYHDFHRHPAGRHVIKLCRAEACQSMGAEALA
                     ALVQRSLKVTFGETTADGAITVEAAYCLGNCALAPAMMVDGRLVGRVDAEGFEKIAAE
                     CRA"
     gene            complement(2407..2622)
                     /locus_tag="AC16A0_06160"
     CDS             complement(2407..2622)
                     /locus_tag="AC16A0_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104950.1"
                     /translation="MSLFSNPPAAHCEKEGRSVRLNQTEGQCRDSHRCGDDPCPLECR
                     FAEGRFASALEMLAASLGQGLVAREGK"
BASE COUNT          488 a          893 c          938 g          490 t
ORIGIN      
        1 ggtctgcgtc ttgaccttga tgccgtgggc aacgggcgtg gtgcaggagg cgggcgttcc
       61 cttgcggcct tcgatctcga cgaggcagag gcggcaggaa ccgaagcttt cgagcgtatc
      121 ggtggcgcag agcttcggga tcttggtgcc cagctccatt gcggcgcgca tgatggaggt
      181 tccctccggc acggtgaccg aaaggccgtc gatctccagc gtcaccaggt tgggcgagcc
      241 ggacttcggc gttccgtagt cggtttcctt gatgagggtc attcctgtcg ctcctactcg
      301 gccgccatgc gggccggctt gtcgaaatcc tcggggaagt gcttcaccgc gctcatgacc
      361 ggcatggggg tgaggccgcc catggcgcag agcgagccgt cggtcatggt ctgacagaga
      421 tcctccagca cctcgaggtt ggcgtcgcgg tcgacgccgg cgataatctt gtcgatggtc
      481 tcgaccccgc gggtcgagcc gatgcggcag ggcgtgcact tgccgcagga ttcgatggcg
      541 cagaactcca tggcgaagcg cgccatgcgg gccatgtcga cgctgtcgtc gaagaccacg
      601 atgccgccgt ggcccaccat tgcgccctgc gcggcgaagg cctcgtaatc catcggcagg
      661 tcgagctgct cggccggcag ataggcgccg agcgggccgc ccacctggat ggcgcggatc
      721 gggcgaccgg tgaaagtgcc gctgccgaag ccctcgacca gttcccgcag cgtgatgccg
      781 aaggccttct cgacaaggcc gcccttgcgg atgttgccgg ccagctggaa gggcagggtg
      841 ccgcgcgagc gtcccatgcc gtagtctttg tagaccgtgc cgccctttgc gagaatgaag
      901 ggaacggccg cgaagctcag cacgttgttg atgacggtcg gcttgccgaa caggccttcc
      961 agcgccggga tgggcggctt cggccgcacc atgccgcgct tgccctcaag gctttccagc
     1021 atggcggtct cttcgccgca gatataggcg ccggcgccgc ggcggacctc gaggcggaat
     1081 gatttgtccg taccctgaat attgtcgccc agccacttcg ccgcgactgc ggcggcgatg
     1141 gcctccttca tggcggcgat ggcgtcggga tactccgagc ggatgtagac gtagccccgc
     1201 gtggcgccgg tggcgatgcc ggcgatggtc atgccctcga tgagcatgag gggatcgccc
     1261 tccatgacca tgcggtcggc gaaggtgccg ctgtcgccct cgtcggcgtt gcagcagaca
     1321 tacttctggt ccgccttcgc atcatgcacc gtcttccact tgatgccggt agggaagccc
     1381 gcgccgccgc ggccgcgcag tcccgactcg gagacctcgg cgacgatggc cgcgcccgcc
     1441 atggacagtg ccttcttcaa tccttcgaag ccgccatgct tcacatagtc ctcgaccgag
     1501 accggatcgg tgatgccgca acgtgcgaag gtgaggcgtt cctggttctt caggtagggg
     1561 tgggactcga tgttgcccag ggacagcgcg tgtgtgccgc cctcatggaa cttcgcggcg
     1621 accagcgagt cgacgtcggc ggcgctgacg ggtccatagg cgacgcgccc ggatggggta
     1681 tcgacctcca ccatcggttc cagccagaac aggccgcgcg atccgttgcg gacgatctgc
     1741 aggccgagat gctgcgcatg cttcgcgatg gccgcagcca cctcatcggc gcccaccgag
     1801 cgggcggcgg actcgcgggg accgaaaacg cggatcatgc gcggcactcc gcggcaatct
     1861 tctcgaagcc ttcggcatcg acccggccga cgagcctgcc atccaccatc atggcgggcg
     1921 ccagcgcaca gttcccgagg caataggcag cctcgacggt gatggcgcca tcggcggtgg
     1981 tttcgccgaa cgtcaccttg agcgaccgct gcaccaatgc ggcgagggcc tcggccccca
     2041 tcgactggca ggcctcggcc cggcagagct tgatgacgtg gcggcccgcc gggtgacggt
     2101 ggaagtcgtg atagaaggtc acgacgccat ggacctcggc gcgcgagagg ttcagcgcct
     2161 tggccagcgc ggggagcgcc gactcgggca catgcccgag ttccgcctgg agatcgtgca
     2221 ggatctcgat gagcgcatct ggcttgttgc catgggccaa ggcaatgcgc cgtgcgtcca
     2281 gcgcttcctt gggcgaagtg tcaatcaggc tatacgtttg cgacatggac attctatcgc
     2341 ccaagcaggc gaaaaacgcc aatcgactga tcctatggga tcatagacgc cagtgatgac
     2401 aagggtttat ttaccctcgc gggccaccag cccctgacca aggctggccg ccagcatctc
     2461 cagcgcggac gcaaaacggc cctcggcgaa gcggcattcc agcgggcagg gatcgtcgcc
     2521 gcagcgatgg gagtcgcggc actgcccttc cgtctggttc agccgaacgc tgcggccttc
     2581 cttttcgcaa tgggcggcag ggggattgga aaacaggctc atggattcat ctccttaaat
     2641 aatcaatcgc ccagccgcag ggcggaatcc ttgatcctga tcaagcgcgg ctcgccacaa
     2701 aaatcctagt aaacggaaaa tagtccttga cagcgtacac tcggcaaggt aagaaggcgc
     2761 atactccaca catgggtcaa gagatgagcg acggcgaagt gaagacggc
//
LOCUS       DBNGVR010000287         3533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4621861, whole genome shotgun sequence.
ACCESSION   DBNGVR010000287 DBNGVR010000000
VERSION     DBNGVR010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4621861"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..935)
                     /locus_tag="AC16A0_06165"
     CDS             complement(12..935)
                     /locus_tag="AC16A0_06165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3104951.1"
                     /translation="MILCCGEALIDFLPRTGKDGATLYQPFNGGSVYNTAIALGRLGN
                     RTGLFTGLSTDFFGDMLREGLKASKVDLRYIKTWDRPSTLAFVKLTDGHARYSFFDDN
                     SAGRMLSRRDLPRLEKAVSALHFGSISLIPEPGGATLEALLKREARSRVITLDPNIRP
                     GMIRERRKHMARLNRMIAMADILKISDEDVAWMTGKADVAAAARKWLKAGAKIVVVTR
                     GGNGVEAFTARDRISRSAVKVKVADTVGAGDTFTAGLITALQRAKLLTKTKLGGISAA
                     DLDAALAFAARAAAVTVSRPGCDPPWASELA"
     gene            1096..2577
                     /gene="zwf"
                     /locus_tag="AC16A0_06170"
     CDS             1096..2577
                     /gene="zwf"
                     /locus_tag="AC16A0_06170"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3104952.1"
                     /translation="MTPRIIPVESFDLVVFGATGDLAQRKLLPALFHRDLQGQIPPGA
                     RIIGASRRAMSNEEFHGFAEAAIKAHVPADQLAGDVMARFLARLSYVSVDASRDADWD
                     DLAAALGETGDRIRVFYLAVGPDLFGPICERLGSHGLVTPQTRVVIEKPLGKSGASAR
                     AVNDAIGKVFSEPQIYRIDHYLGKETVQNLMALRFANALFEPVWNSAHIDHVQITVAE
                     TLGVEGRAGYYDTAGALRDMVQNHILQLLCLVAMEPPASAAANDLRDEKLKVLKALRP
                     IMGDAVAQLTVRGHYRAGASDGGAVKGYLEELGSTTSKTETFVAIKAEIENWRWAGVP
                     FYLRTGKRLPSRVSEIVVTFKPVPHSIFGTAAGPVMSNRLVLRLQPDEGVKLWLMIKD
                     PGPGGMRLSHVPLDMTFAEAFDVRNPDAYERLLMDVVRGSQTLFMRRDEVEAAWAWID
                     PILEAWAASPDLPKPYTAGTWGPSAAVALIERDGRTWYEDQDL"
     gene            2574..3230
                     /locus_tag="AC16A0_06175"
     CDS             2574..3230
                     /locus_tag="AC16A0_06175"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3104953.1"
                     /translation="MIEAERIFPAREELAESLAHDVAEELMRAIEAKGKATLAVSGGT
                     TPKTFFAKLSQIDLPWHRVTVTLVDERQVPETSERSNAKLVRETLLVDRAAAAKFVPL
                     IDTDAAAALPPFDVTVLGMGADGHTASFFPGGDRLAEAMNSNTQARIIAMTAPGAGEP
                     RLTFTLPVIEATGRLKLHIEGAEKKDVLARALADGPTAELPVRAVLRSQTPLTLYWAP
                     "
     gene            3246..>3533
                     /locus_tag="AC16A0_06180"
     CDS             3246..>3533
                     /locus_tag="AC16A0_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HIR3104954.1"
                     /translation="MPVKATISAVTEAIIARSAAPRRRYLDRIDAAVARQPKRKALGC
                     ANIAHGFAACGAADKNALRNGTGPNLGIVTAYNDMLSAHQPFETYPELIRKA"
BASE COUNT          643 a         1151 c         1150 g          589 t
ORIGIN      
        1 cgcctcaaat gtcaagcaag ctcgctggcc cagggcgggt cgcaacccgg ccgcgagacg
       61 gtgaccgccg cggcccgcgc cgcaaaggcc agcgccgcat cgagatcggc ggccgaaatg
      121 ccgcccagct ttgtcttggt cagcagcttc gcccgctgca gggcggtgat gaggcccgcc
      181 gtaaacgtgt cgccggcacc cacagtgtcc gccaccttca ccttcaccgc agaccgggag
      241 atgcggtcac gcgccgtgaa ggcctcgaca ccattaccgc cacgggtgac gacgacgatc
      301 ttcgcgccgg ccttgagcca tttgcgggcc gcagccgcga catcggcctt gcccgtcatc
      361 caggcgacat cctcgtccga gatcttgagg atatcggcca tggcgatcat gcggttgagg
      421 cgcgccatgt gcttgcggcg ctcccggatc atgccggggc ggatattggg atcgagggta
      481 atgacacggg atcgcgcctc gcgcttcaac agcgcctcca atgtcgcgcc gcccggttcg
      541 gggatcaggc tgatggagcc gaaatgcagg gcggaaacgg ccttctcgag ccgcggcaga
      601 tcccggcggg acagcatgcg gccggcggaa ttgtcgtcga agaaggaata gcgggcatgg
      661 ccgtcggtga gcttcacgaa ggcaagggtg gacggccggt cccaggtctt gatgtagcgc
      721 aggtccacct tgctggcctt gagaccctcg cgcagcatgt cgccgaagaa atcggtggac
      781 agtccggtga agaggcctgt ccggttgccc agccggccca gcgcgatggc cgtgttgtag
      841 accgagccac cattgaaggg ctgatagagc gtggcgccat ccttgcccgt ccggggcagg
      901 aagtcgatca gggcttcccc acagcagaga atcatccaaa ggcctcccag aatatctttg
      961 tttttcaatg cttatgccgc atttttcaac acaaggcaat aaatcaattc agttgaatta
     1021 tcgccataat gtttgactat gcggagaaac agtctatctg agcggcgcac atcaaatcca
     1081 gcggaaagac cctcaatgac cccccgcatc attcccgtgg agagcttcga ccttgtggtg
     1141 ttcggggcca cgggcgacct tgcgcagcgc aagcttctgc cggcgctctt ccatcgcgac
     1201 ctgcagggac agattccgcc aggcgcgcgg atcatcgggg catcgcgccg ggccatgagc
     1261 aatgaagaat tccatggttt tgccgaggcg gcgatcaagg cgcatgtgcc ggccgatcaa
     1321 cttgcgggcg acgtcatggc acggtttctc gcccggctca gctacgtctc ggtcgacgcg
     1381 tcgcgcgatg ccgactggga cgatctcgcc gcagcactgg gcgaaacggg cgaccgcatc
     1441 cgcgtcttct atctggccgt cggtcccgac ctcttcggac cgatctgcga gcggctgggc
     1501 agccacgggc tggtgacgcc gcagacccgc gtggtgatcg agaagccgct gggcaagtcc
     1561 ggcgcctcag ccagggccgt gaacgacgcc atcggcaagg tcttctccga gccgcagatc
     1621 tacagaatcg atcattatct cggcaaggag accgtgcaga acctgatggc gctgcgcttc
     1681 gccaatgcgc tgttcgagcc cgtctggaac tcggcccata tcgatcacgt gcagatcacc
     1741 gtggccgaga ctctcggcgt tgagggccgt gccggttact acgacacagc cggcgcgctg
     1801 cgtgacatgg tgcagaacca catcctgcag ctgctttgtc tcgtggccat ggaaccgccc
     1861 gcctctgccg ccgccaatga cctgcgcgac gagaagctga aggtgctcaa ggcgctgcgg
     1921 ccgatcatgg gcgatgccgt ggcgcagctc acggtgcgcg ggcactatcg cgccggcgcg
     1981 tcggatggcg gcgcggtgaa gggctatctg gaggaactgg gatcgacaac gtccaagacc
     2041 gagactttcg tcgccatcaa ggccgagatc gagaactggc gctgggccgg cgtgcccttc
     2101 tatctgcgca ccggcaagcg ccttccgtcg cgcgtttccg agatcgtggt gaccttcaag
     2161 ccggtgccgc attcgatctt cggcacggcc gccggtccgg tgatgtcgaa ccgcctggtg
     2221 ctgcgcctgc agcctgacga gggcgtgaag ctgtggctga tgatcaagga ccccggcccg
     2281 ggcggcatgc gcctctcgca tgtgccgctg gacatgacct tcgccgaggc cttcgacgtg
     2341 cgaaatccgg atgcctatga acgcctgctg atggacgtgg tccgcggcag ccagacgctg
     2401 ttcatgcgcc gtgacgaagt ggaggccgca tgggcctgga tcgatccgat cctggaggcc
     2461 tgggcggcat cccctgacct gcccaagccc tatacagccg gcacctgggg gccttccgcc
     2521 gccgtggcgc tgatcgagcg ggacgggcgc acctggtacg aggatcaaga cctatgatcg
     2581 aagccgaacg aatctttccc gcgcgcgagg agctggccga gagcctggcc catgacgtgg
     2641 ccgaagagct gatgcgcgcc atcgaggcca agggcaaggc gaccctggcc gtttcgggcg
     2701 gcacgacacc taagaccttc tttgccaagc tgtcgcagat cgatctgcca tggcatcgcg
     2761 tgacggtgac gctggtcgac gagcgccagg tgcccgagac gagcgagcgg tccaatgcga
     2821 aactggtgcg cgagacgctg ctggtcgaca gggcggcggc cgcgaaattc gtgccgctga
     2881 tcgataccga cgctgccgcg gccctgccgc ccttcgacgt gaccgtgctc ggcatgggcg
     2941 ccgacgggca tacggcttcc ttctttccgg gcggcgaccg gctggccgaa gcgatgaaca
     3001 gcaataccca ggcccgcatc atcgcaatga cggcgccggg cgccggcgag ccacgcctca
     3061 ccttcacact tccagtaatc gaggcgacag gacggctgaa gcttcacatc gaaggcgcgg
     3121 aaaagaagga cgtgctcgcg cgcgccctgg ccgatggtcc gaccgcggaa ttgccggtgc
     3181 gggcggtctt gcgcagccag acacccttga ccctatactg ggccccctag caggagacgc
     3241 catccatgcc ggtcaaggcc acgatatccg ccgtcaccga agcgatcatc gcgcgcagcg
     3301 cggcgccgcg gcggcgctat ctcgacagga tcgatgcggc agtggcgcgg caaccgaagc
     3361 gcaaggcgct gggctgcgcc aacatcgccc atggctttgc cgcctgcggc gcagccgaca
     3421 agaacgcgtt gcgcaatggg accggtccca atctcggtat cgtcacggcc tacaacgaca
     3481 tgctgtcggc gcatcagccc ttcgagacct accccgaact gatccgcaag gcg
//
LOCUS       DBNGVR010000288         1904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_300676, whole genome shotgun sequence.
ACCESSION   DBNGVR010000288 DBNGVR010000000
VERSION     DBNGVR010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_300676"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..986)
                     /locus_tag="AC16A0_06185"
     CDS             complement(153..986)
                     /locus_tag="AC16A0_06185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3104955.1"
                     /translation="MNVGARIAREAELAMILDWARIEGWNPGLDDAPPFWAADPRGYF
                     LAEVDGQPAAAISVVNHDDTFAFLGLYLCQPSFRGRGIAYGLWQHALAHAGNRTLGLD
                     GVPAQQDNYRKSGFVSAGSTLRMQGDVPGLQDAAVRPAVAADTSRITELDRSANGYDR
                     KTFLVAWIAPGARRRTLVLHEGGEVAGYATVRLCHAGIKIGPVVAPDADAAMRLIRAA
                     AGVLGSPAAMIDVPAQNQALRDRLSAAGLVPSFPTARMYRGTPPRQDGKLQAVGTLEL
                     G"
     gene            complement(997..1773)
                     /locus_tag="AC16A0_06190"
     CDS             complement(997..1773)
                     /locus_tag="AC16A0_06190"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR3104956.1"
                     /translation="MAEFVLPKNSRVQPGKVWPKPQGKRLRDFRIYRWNPDDGKNPQV
                     DTYTLDMDKCGPMVLDALIKIKNEQDPTLTFRRSCREGICGSCAMNIDGTNTLACLKS
                     CADVKGDVRIYPLPHMPVIKDLVPDLTRFYAQHRSIEPWLKTTTPEPQTEWRQSKEDR
                     AKLDGLYECILCACCSTSCPSYWWNGDRYLGPAVLLQAYRWLIDSRDESTGERLDNLE
                     DPFRLYRCHTIMNCAKACPKGLSPARAIAEIKKMMVERRI"
BASE COUNT          332 a          585 c          651 g          336 t
ORIGIN      
        1 aataggtata ggtcttcgca ccgaccttga gggtcttctt ggacttgaag ctgtcgaggg
       61 acatcggggc ctcatgatgc tggggatttg gcgtgagcct atagtatctt ttgcactgca
      121 acaccagagc aacctagggc gtaagcggcg cttcagccga gctccagcgt gcccaccgcc
      181 tgcagctttc catcctgccg cggcggcgtg ccgcgataca tgcgcgctgt cggaaaactg
      241 ggcacgaggc cggccgcaga gagccggtcg cgcagcgcct ggttctgggc agggacatcg
      301 atcatggccg caggcgagcc gagaacgcct gccgctgccc ggatgagccg catggcggca
      361 tccgcatcgg gcgccaccac cggcccgatc ttgatgccgg catggcagag acggacggtg
      421 gcatagccgg ccacctctcc gccctcgtgc agcaccaggg tgcgccgccg cgcgccgggt
      481 gcgatccaag ccacgaggaa ggtcttgcgg tcatagccat tggccgaccg gtcaagttcc
      541 gtgatgcgcg aggtatcggc cgccacggcg ggccgcacgg ccgcatcctg caggcctggc
      601 acgtcaccct gcatgcgcaa ggtggaaccg gccgagacga aacccgactt gcggtaattg
      661 tcctgctggg ccggaacgcc gtcgaggccc agcgtgcggt tgcccgcatg cgccagcgca
      721 tgctgccaga gaccataggc gatgccgcgt ccccggaacg aaggctggca gaggtagagg
      781 cccaggaacg cgaaggtgtc gtcgtgattg accaccgaaa tcgccgcagc cggctgacca
      841 tcgacctcgg cgagaaaata tccccgcgga tcggcggccc agaagggcgg cgcatcgtcg
      901 aggccgggat tccagccctc gatgcgcgcc cagtccagga tcatcgcgag ttcggcttcg
      961 cgcgcgattc tcgccccgac attcatgagg cgtccctcag atgcggcgct ccaccatcat
     1021 cttcttgatc tcggcaatgg cccttgccgg cgacaggcct ttggggcagg ccttggcgca
     1081 gttcatgatg gtgtggcagc gatagagccg gaagggatcc tcgagattgt ccaggcgctc
     1141 gcccgtggat tcatcgcgcg agtcgatgag ccagcgatag gcctgcagca gcacggcggg
     1201 accaagatag cggtcgccgt tccaccagta ggacgggcag gaggtggagc agcaggcgca
     1261 gaggatgcat tcgtagagcc cgtcgagctt ggcgcggtcc tccttggact ggcgccactc
     1321 ggtctgcggt tcgggcgtcg tggtcttgag ccagggctcg atcgagcggt gctgcgcata
     1381 gaagcgcgtg aggtcgggca ccaggtcctt gatcaccggc atgtgcggca gcggatagat
     1441 gcggacatcg cccttcacgt ccgcgcagga cttgagacag gccagcgtgt tggtgccgtc
     1501 gatgttcatg gcgcaggagc cgcagatgcc ttcgcggcag gagcggcgga aggtgagcgt
     1561 cgggtcctgt tcgttcttga tcttgatcag cgcgtccagc accatcggac cgcacttgtc
     1621 catgtcgagc gtgtaggtgt cgacctgcgg gttcttcccg tcatcggggt tccagcggta
     1681 gattcggaaa tcccggagcc gcttgccctg gggcttgggc cagaccttgc cgggctggac
     1741 cctggagttc ttgggaagga cgaattcagc catgggaatg atccgagtca gatttcaccc
     1801 ccgcccgcgg cgagagcgaa gagaaattca tgttgagcgc ggcaggcatc aatacacccg
     1861 cgccttgggc ttgatgtagg cgatgtcgtc gctcagcgtg tagt
//
LOCUS       DBNGVR010000289         2715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2264786, whole genome shotgun sequence.
ACCESSION   DBNGVR010000289 DBNGVR010000000
VERSION     DBNGVR010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2264786"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /gene="edd"
                     /locus_tag="AC16A0_06195"
     CDS             <1..1623
                     /gene="edd"
                     /locus_tag="AC16A0_06195"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HIR3104957.1"
                     /translation="PNLGIVTAYNDMLSAHQPFETYPELIRKAARDSGGTAQVAGGVP
                     AMCDGVTQGETGMELSLFSRDVIALSTAVALSHQMFDAAVYLGVCDKIVPGLIIGALS
                     FGHLPAVFIPAGPMTSGLPNDEKGKIRQLYAEGKVTRDALLEAEAQSYHGPGTCTFYG
                     TANSNQMLMEIMGLHLPGSTFVNPGTPLRDAITRASAKQALSITALGNDYRPIGRIYD
                     EKAVVNGIVGLLATGGSTNHTLHLIAMAAAAGIVVTWKDFAELSAAVPLLSRVYPNGK
                     ADVNHFNAAGGMGFLIRELLGAGLLHEDVQTIMGTGLAGYTKEARLGADGELVYADAA
                     AISGDESVLRPSARPFQPTGGLNVLDGNLGTAVIKSSSIPADRHVIEAPARVFHSQEE
                     LQKAFKSGELNCDVVAVVRFQGPKANGMPELHRLTPPLAVLQEKGFRVALVTDGRMSG
                     ASGKVPAAIHLTPEAAEGGPIAKIRDGDMIRVDADAGTLEVLVGAAEFHARPPVHIDL
                     SANHQGTGRELFAAFRAITGSAAQGASIFEVA"
     gene            1625..2266
                     /gene="eda"
                     /locus_tag="AC16A0_06200"
     CDS             1625..2266
                     /gene="eda"
                     /locus_tag="AC16A0_06200"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR3104958.1"
                     /translation="MARDYQKGLEAALKRAPVVPVMVIEEVADAVPLARALVKGGLPV
                     LEITLRTPKAMECIRAIVAEVEDAIVGSGTVLTPEQLRDSEKLGCKFAVSPGSTGKLL
                     GAAEDHDIALLPGGVTASECMALLEWGYAIQKFFPAEPAGGTAYLSSLASPLPQVRFC
                     PTGGITPESATRYLKLSNVITIGGSWMAPKTLVAAKDWAGIEALARQAASLKA"
     gene            complement(2267..>2715)
                     /locus_tag="AC16A0_06205"
     CDS             complement(2267..>2715)
                     /locus_tag="AC16A0_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3104959.1"
                     /translation="PAMLLSVMAAICGALYNIATRKVGGRDRAETSLFYVGFVGSLGA
                     ALPLPWHWQMPEGTQWLFLVGMGLCGGIGHLMLIQAHRLAPAAALSPFVYTQIVWMIA
                     LGFLMFGDVPDLWTLIGAAVVIASGLFVFARERSLGRSGEVPAPVD"
BASE COUNT          459 a          907 c          920 g          429 t
ORIGIN      
        1 cccaatctcg gtatcgtcac ggcctacaac gacatgctgt cggcgcatca gcccttcgag
       61 acctaccccg aactgatccg caaggcggcg cgcgattcgg gcggcacggc gcaggtggcc
      121 ggcggcgtgc ccgccatgtg cgacggcgtg acccagggcg agaccggcat ggagctgtcg
      181 ctgttttcgc gcgacgtgat cgcgctgtcg acggcggtgg cactgtcgca ccagatgttc
      241 gatgcggccg tctatcttgg cgtctgcgac aagatcgtgc ccggactcat catcggtgcc
      301 ttgtccttcg ggcacctgcc cgccgtgttc atcccggccg gcccgatgac cagcggtctg
      361 ccgaacgacg agaagggcaa gatccgccag ctctacgccg agggcaaggt gacgcgcgac
      421 gcgctgctgg aagccgaggc gcagtcctat cacggccccg gcacctgcac tttctacggc
      481 actgccaatt ccaaccagat gctgatggag atcatggggc tccatctgcc gggttcgacc
      541 tttgtcaatc ccggcacgcc gctgcgcgat gccatcaccc gtgcctcggc gaagcaggcc
      601 ctgtcgatca cggcacttgg caacgactac cggccgatcg gccgcatcta tgacgagaag
      661 gcggtggtca acggcatcgt cgggctcctc gccaccggcg gttcgaccaa ccacacgctg
      721 catctgatcg ccatggccgc ggcggccggc atcgtcgtca cctggaagga ctttgccgaa
      781 ctctccgccg ccgtgcccct gctgagccgc gtctatccga acggcaaggc cgacgtgaac
      841 cacttcaatg cggccggcgg catgggcttt ctcatccgcg aactgctggg cgccgggctg
      901 ctgcacgagg atgtgcagac catcatgggc acgggcctcg ccggctatac caaggaggca
      961 cggctcggag cggacggcga gcttgtctat gccgatgccg cggcgatctc cggcgacgaa
     1021 agcgtgctgc gcccgagcgc gcggcccttc cagcccaccg gcggcctcaa tgtgctcgac
     1081 ggcaatctcg gcaccgccgt gatcaagtcc tcctccattc ctgccgaccg gcatgtgatc
     1141 gaagcgccgg cccgggtgtt ccactcgcag gaggagctgc agaaggcctt caagtcgggc
     1201 gagctgaatt gcgacgtcgt cgccgtggtg cgcttccagg gtcccaaggc caacggcatg
     1261 cccgaactgc atcggctcac gccgccgctg gcggtgctgc aggagaaggg tttccgggtt
     1321 gcgctcgtca ccgatggccg catgtcgggc gcatccggca aggtgccagc cgccatccat
     1381 ctcacgccgg aggcggccga gggcgggccg atcgcgaaga ttcgcgacgg cgacatgatc
     1441 cgggtcgacg ccgatgccgg gacgctcgag gttctggttg gagcggcaga gttccatgcg
     1501 cgcccgccgg tgcacatcga tctgtcggcg aaccaccagg gcacgggccg ggaactcttt
     1561 gcggccttcc gcgccatcac cggttccgcc gcacagggcg catcgatctt cgaggtggcc
     1621 tgagatggcg cgcgactacc agaaaggtct tgaggccgcg ctgaagcggg cgccggtcgt
     1681 gccggtcatg gtgatcgagg aggttgccga cgcggtgccg ctggcacgcg ccctggtcaa
     1741 gggcgggctg ccggttctcg aaatcacctt gcgtacaccc aaggccatgg agtgcattcg
     1801 tgccatcgtc gccgaagtcg aggacgccat cgtcggatcg ggcacggtgc tgacgcccga
     1861 acagttgcgc gacagcgaaa aactgggctg caaattcgcg gtctcgccgg gctcgaccgg
     1921 caagcttctt ggcgcggcgg aagaccatga catcgcgctc ttgccagggg gcgtgacggc
     1981 gtcggaatgc atggccctgc tcgaatgggg ctatgccatc cagaagttct tccccgcgga
     2041 accggccggc ggcacggcct atctgtcgtc gctcgcctcg cccctgcccc aggtgcgctt
     2101 ctgccccacg ggtggcatca cgccggaatc ggcgacgcgc tatctgaagc tgtccaacgt
     2161 gatcaccatc ggcggatcgt ggatggcgcc gaagacactc gttgccgcca aggactgggc
     2221 gggaatcgag gcgcttgcgc ggcaggcggc ttcgctcaag gcctgatcag tcgacaggtg
     2281 cgggaacctc gccgctgcgg ccgaggctgc gctcgcgggc gaagacgaag aggccgctgg
     2341 cgatgaccac tgcggcgccg atcagggtcc agaggtcagg cacgtcgccg aacatcagga
     2401 agcccagtgc gatcatccag acgatctggg tatagacgaa gggcgagagc gccgcggcag
     2461 gcgccaggcg atgcgcctgg atcagcatga gatggccgat gccgccgcag agccccatgc
     2521 cgacgaggaa cagccactgg gtgccttcgg gcatctgcca gtgccagggc agcggcaggg
     2581 cggcgccaag actgccgacg aaacccacgt agaagagcga ggtctcggcc cggtcgcggc
     2641 cgccgacctt gcgcgtggcg atgttgtaga gcgccccgca gatcgccgcc atgaccgaga
     2701 gcagcatggc aggat
//
LOCUS       DBNGVR010000290         3114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1283552, whole genome shotgun sequence.
ACCESSION   DBNGVR010000290 DBNGVR010000000
VERSION     DBNGVR010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1283552"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16A0_06210"
     CDS             complement(<1..194)
                     /locus_tag="AC16A0_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR3104960.1"
                     /translation="MFLGIDIGTSAVKALLVDGDENGLATATVPVATSRPHPLWSEQD
                     PDDWWRATTDAVAQLRAQAGP"
     gene            complement(206..958)
                     /gene="ubiG"
                     /locus_tag="AC16A0_06215"
     CDS             complement(206..958)
                     /gene="ubiG"
                     /locus_tag="AC16A0_06215"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR3104961.1"
                     /translation="MTAAAPSLDPSEVEKFSKMAAEWWNPKGKFGVLHVFNPVRLQYI
                     KEQVCGRLGLDPLERQPFAGLRFLDIGCGGGLLCEPMARLGATVVGVDPSEKNIKTAT
                     VHADEVGLAIDYRVGTTEDLAAAGERFDVILNMEVIEHVADPAAYIGSCAAMLKPGGL
                     MFVATLNRTLKSFGLAIVGAEYVLGWLPKGTHQWEKFITPEELRGWLATNQADIRDEV
                     GVTYSPFSGTWRKSRDMAVNYMVVAEKSADAA"
     gene            1123..2379
                     /locus_tag="AC16A0_06220"
     CDS             1123..2379
                     /locus_tag="AC16A0_06220"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3104962.1"
                     /translation="MGRLVMKFGGTSVADIDRIRNVARHVEREVKAGHHVAVVVSAMS
                     GTTDRLVGWCREAAAVHDAREYDAVVASGEQVTSGLLAIVLQDMGIPARSWAGWQIPI
                     KTDGVHGAARIAAIDGAELRRRMEQGQVAVIAGFQGIGPDRRITTLGRGGSDTSAVAV
                     AAALDAACDIYTDVDGVYTADPRIVSSARKLDRISYEEMLEQASLGAKVLQTRSVELA
                     MVYKVPLQVRSSFEPPDSPDQNKPDGTGPGTLVCDEERIVEQHVVSGIAYSRDEAKVA
                     IRKVKDQPGVSASLFGPLAEADISVDMIVQNISADGSTADITFTVARKDLRKTLEVLS
                     GVKDKIGFADIQHSADVAKVSVVGIGMRSHAGVAAKMFRSLAEKGINIQAITTSEIKV
                     SVLIDEAYTELAVRALHTAYGLDAKH"
     gene            2467..>3114
                     /locus_tag="AC16A0_06225"
     CDS             2467..>3114
                     /locus_tag="AC16A0_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3104963.1"
                     /translation="MAGSALGPRVLLKRLREVMAEPVAAQKRLDKIVKVIAANMVAEV
                     CSIYVMRPGDVLELYATEGLKGEAVHKSKLKTGEGLVGTIAEEAIGLNLAEAQDHPAF
                     KYLPETGEEIYHAFLGVPIMRAGTVIGVLVVQNRTRRHYTEEEEEALQTTAMVLAEVI
                     ASGELREVASEVAADVAHVRSHVIRGDSLAEGVALGYAVLHEPRVVILNLIAENVP"
BASE COUNT          572 a          993 c         1006 g          543 t
ORIGIN      
        1 ggtccggcct gcgcccgcag ctgggccacg gcatccgtgg tggcacgcca ccagtcatcg
       61 ggatcctgct cggaccagag cgggtgcggc cgcgacgtgg cgacggggac cgtagccgtc
      121 gcaaggccgt tctcgtcacc gtcgacaagc agggccttga cggcagacgt cccgatgtcg
      181 atgccaagaa acacggggag cgccgctaag ccgcgtcagc ggatttttcg gcgacgacca
      241 tataattcac ggccatgtcc ctgctcttgc gccaggtgcc cgagaacggg ctgtaggtca
      301 ctcccacttc atcgcgaatg tcggcctggt tcgtggccag ccagccccga agttcttccg
      361 gcgtgatgaa cttttcccac tgatgcgtgc ccttgggcag ccagcccagc acatactccg
      421 cgcccacgat ggcgagtccg aaggacttga gcgttcggtt cagggtggcc acgaacatca
      481 ggcccccggg cttcaacatg gcggcacagc tcccaatgta ggctgcggga tcggccacgt
      541 gctcgatcac ctccatgttg aggatgacgt cgaaccgctc gcccgcagcc gcgaggtcct
      601 cggtggtgcc gacacgataa tcgatagcga gtccgacctc gtctgcatgc accgtcgcgg
      661 tcttgatgtt cttctcggac gggtcgacgc ccaccacggt cgcgccgagc ctggccatcg
      721 gttcgcaaag aagtccgccg ccgcagccga tgtcgagaaa ccgcaagccg gcaaaaggct
      781 ggcgctcgag gggatcaagt ccaaggcggc cgcagacctg ctccttgatg tactgcaggc
      841 gcacggggtt gaagacatgg agcaccccga acttgccttt cgggttccac cattcggcgg
      901 ccattttcga gaatttctcg acctctgacg gatcgagcga aggcgcggca gctgtcattt
      961 gcggattccg gaaaattgct cgagccatta ttgcataagc cagggctgtc atctatgagt
     1021 acgccgcgcc gcacccgggg gttcagtcga cgatcagatt tggccactgg ccggactctt
     1081 tcgcagggtt cggacggcag ggacattgaa aggtttgggc cgatgggccg tttggtcatg
     1141 aagttcggtg ggacgtcggt tgcggacatc gaccgcatcc gcaacgtcgc gcgccatgtc
     1201 gaacgcgagg tcaaggccgg ccaccacgtg gcggtcgtgg tctccgccat gtcgggcacc
     1261 actgaccgcc tggtcggctg gtgccgcgaa gccgcagccg tgcatgacgc acgcgagtac
     1321 gatgctgtcg tggcctctgg cgaacaggta acctcgggtc tgctcgcgat cgttctgcag
     1381 gacatgggca ttcccgcgcg gtcctgggcc gggtggcaga tcccgatcaa gaccgatgga
     1441 gtccatggtg cagcccgcat cgccgccatc gatggcgcgg aactgcgccg ccgcatggaa
     1501 cagggtcagg tggcggtgat cgcaggcttc cagggaattg gccccgaccg ccgcatcacc
     1561 acgctgggcc gcggcggatc ggacacctcg gcggtggccg tggcagcggc gctcgatgcg
     1621 gcctgcgaca tctataccga tgtcgatggc gtctataccg ccgatccgcg catcgtctct
     1681 tcggcccgga aactggaccg catttcctac gaggagatgc tagaacaggc ttcgctgggt
     1741 gccaaggtgc tgcagacccg gtcggttgaa ctggccatgg tctacaaggt accattgcag
     1801 gtacgctcga gcttcgagcc gcccgactcg cccgaccaga acaagcccga tgggaccggc
     1861 cccggaacac tcgtttgcga tgaggaacgt atcgtggaac agcatgtcgt cagtggtatc
     1921 gcctattcgc gcgacgaggc gaaggtcgcc atccgcaagg tcaaggacca gccgggagtc
     1981 tccgcctcgc tgttcggccc gctggcggaa gccgacatca gcgtcgacat gatcgtgcag
     2041 aacatctctg ccgacggttc gacggcggac atcaccttca cggtggcgcg caaggatctg
     2101 cgcaagacgc tggaagttct cagcggcgtg aaggacaaga tcggctttgc cgacatccag
     2161 cattccgccg acgtggccaa ggtctcggtg gtgggcatcg ggatgcgcag ccatgccggc
     2221 gtggccgcca agatgttccg ctcgctggcg gagaagggaa tcaacatcca ggcgatcacc
     2281 acttcggaga tcaaggtgag cgtgctgatc gacgaggcct ataccgagct cgcggtgcgc
     2341 gccctgcaca ccgcctatgg actggacgca aagcactgaa ccgatgcgcc tgttcatcac
     2401 ccatagccag cccgactgca tgcgcgtcaa aacggtgcgc gtgaccacgc gcgaggacac
     2461 aggctgatgg ctggctctgc ccttggcccg cgtgtcctgc tcaagcggct ccgcgaggtc
     2521 atggcggagc cggttgctgc gcaaaagcgg ctcgacaaga tcgtcaaggt catcgctgcc
     2581 aacatggtgg cagaggtctg ctccatctat gtgatgcggc ctggcgacgt gctggaactc
     2641 tacgccaccg aaggcctgaa gggcgaagcc gtccacaagt ccaagctcaa gaccggcgag
     2701 ggcctcgtgg gcaccattgc cgaggaggcg atcggcctca accttgccga ggcccaggat
     2761 catccggcct tcaagtatct gcccgagacc ggcgaagaga tctatcacgc cttcctcggc
     2821 gtgccgatca tgcgcgccgg cacggtcatc ggcgtgctgg tggtgcagaa ccgcacgcgg
     2881 cgccactaca ccgaagagga agaagaggcg ctgcagacca ccgccatggt gctggccgag
     2941 gtcatcgcct cgggcgagct tcgcgaggtt gcaagcgaag tcgccgccga cgtggctcat
     3001 gtgcgcagcc atgtcatccg cggcgattca ctggccgagg gcgtggcgct tggctacgcg
     3061 gtcctgcacg aaccccgtgt cgtcatcctc aatctcatcg ccgagaatgt gccc
//
LOCUS       DBNGVR010000291         2567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4426033, whole genome shotgun sequence.
ACCESSION   DBNGVR010000291 DBNGVR010000000
VERSION     DBNGVR010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4426033"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16A0_06230"
     CDS             complement(<1..461)
                     /locus_tag="AC16A0_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0043934 - sporulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HIR3104964.1"
                     /translation="MYVPPHFQEISKDEIEAVIDSAPLACIVAQTDNGLIANHIPLLK
                     GPDDTLIGHVALANDMHRLVADDQEVLTIFRGDDAYVSPNFYPSKAEHHRHVPTWNYQ
                     VVHIYGRIAFKHDAQAKRAAVGLLTRHHERRLNGPRAWRMADAPADYMEQML"
     gene            complement(504..839)
                     /locus_tag="AC16A0_06235"
     CDS             complement(504..839)
                     /locus_tag="AC16A0_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HIR3104965.1"
                     /translation="MAISFDDFLKIDIRVGTIVAVDPFPEARKPAFKLKIDFGPEIGV
                     KKSSAQITKYYTPDTLLGRQVAAVVNFPPRQIGPFMSEVLTLGFPDPAGEVVLVAVER
                     EVPNGGRLF"
     gene            complement(843..1664)
                     /gene="proC"
                     /locus_tag="AC16A0_06240"
     CDS             complement(843..1664)
                     /gene="proC"
                     /locus_tag="AC16A0_06240"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3104966.1"
                     /translation="MMVKLDGTLVLLGAGKMGGAMLEGWLKGGADPRKIVAFDPAPPP
                     EMKALLATHGIRLNPPVASINDAEVLVIAVKPQLMEEALPGIVPLKASRPLVLSVAAG
                     KTIAYFEARFGAAAAVVRTMPNTPAAVGRGITAMVTNPNVSAQQAELARTLLGAVGDV
                     VTVDRESLIDAVTGVSGSGPAYVFYLTECLAAAGEKAGLPADIAMKLARATVSGAGEL
                     MRVTGTEAATLRQNVTSPKGTTYAALQVLMSNDGLQPLMDQAVAAATNRSRELAS"
     gene            complement(1661..2161)
                     /locus_tag="AC16A0_06245"
     CDS             complement(1661..2161)
                     /locus_tag="AC16A0_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone family
                     protein"
                     /protein_id="HIR3104967.1"
                     /translation="MPGLEAIDHDLPNPLDRIERLAEAHHWSFDRSTPSEVLMRLEGG
                     WSDLSLSMCWREDLETLQVAAAFDLKVPPPRREEVGRLLALINEQQLHGHFDLWPSDG
                     SIIYRNSLLLAGGAEANDAQCEAMIQLALTACERYFPALQFVIWAGRSAVEALDSSLL
                     ETMGEA"
     gene            2360..>2567
                     /locus_tag="AC16A0_06250"
     CDS             2360..>2567
                     /locus_tag="AC16A0_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3104968.1"
                     /translation="MTETCDFLIVGGGSSGCIVAKRLAERTRGRIILLEAGKPDEGDP
                     AATDLNRLDEQTADYDWGFRAATLR"
BASE COUNT          445 a          836 c          829 g          457 t
ORIGIN      
        1 agcatctgtt ccatgtagtc ggccggcgca tcggccatgc gccaggcccg cggaccgttc
       61 agccgccgct cgtgatggcg ggtgagcagg cccaccgccg cgcgcttggc ctgtgcatcg
      121 tgcttgaatg cgatgcgccc gtagatgtgc acgacctggt agttccatgt cgggacgtgg
      181 cggtggtgtt cggctttcga cggatagaag ttcggagaca cataggcgtc gtcgcccctg
      241 aagatggtca gcacctcctg gtcgtcggca acgaggcgat gcatgtcatt ggccagcgcg
      301 acgtgaccaa tgagcgtgtc atccgggccc ttcagcaaag ggatatggtt ggcaatgagg
      361 ccgttgtcgg tctgtgccac aatacaagcc agaggcgccg aatcgatgac cgcctcgatc
      421 tcgtccttgc tgatctcctg gaaatgcggc ggaacataca tgcgcggctt tccttcattt
      481 gggcgccgtt ctgacgccag gcgctagaac agccgcccgc cgttgggtac ctcgcgttcc
      541 acggcgacca gcacgacttc gcccgcggga tcggggaacc ccagggtcag aacctccgac
      601 atgaaggggc cgatctggcg cggcgggaag ttcacaacgg ccgctacctg ccggccgagc
      661 agcgtgtccg gcgtgtagta cttggtgatc tgggctgaag acttcttcac gccgatctcc
      721 gggccgaagt cgatcttcaa cttgaaggcc ggcttgcgtg cctccgggaa tggatcgacg
      781 gcgacgattg tgcccacgcg gatgtcgatc ttcaggaagt cgtcaaagct gatcgccatg
      841 cttcagctcg cgagctcgcg cgaccggttg gtggcggccg caacagcctg gtccatgagc
      901 ggctgcaggc cgtcattgga catgaggacc tgcagtgcgg cataggtcgt gcctttcggg
      961 ctcgtcacgt tttggcgcag cgtcgccgcc tcggttcccg tcacccgcat cagctcgcca
     1021 gcgcccgata ccgtggcccg cgccagcttc atggcgatgt cggccggaag gcccgctttc
     1081 tcgcccgcgg ccgcaagaca ttcggtaagg tagaagacat aggcggggcc tgatccggac
     1141 acaccggtca cggcgtcgat cagactctcc ctgtcaacgg tcacgacatc gcccacggcg
     1201 ccgagcagcg ttcgcgccag ctcggcctgc tgcgccgaga cgttcggatt ggtcaccatg
     1261 gcggtgatgc cccgccccac cgcagccggc gtattgggca tggtgcgtac gacggcggca
     1321 gcggcaccga atcgcgcctc gaaataggcg atggtctttc cggcggccac cgaaagcacc
     1381 agcggtctcg acgccttcag cggcacgatg cccggaagcg cctcttccat cagctgcggc
     1441 ttgacggcaa tgacaaggac ctcggcgtcg ttgatcgacg cgaccggcgg attgaggcga
     1501 atgccatgcg tggcgagaag cgccttcatc tcgggcggcg gcgcgggatc gaaggccacg
     1561 atctttcgcg gatcggcacc gcccttgagc cagccttcga gcatggcgcc gcccatcttg
     1621 ccggcgccga gcagcaccag cgttccgtcg agcttcacca tcacgcttct cccatggtct
     1681 cgagcaggct cgagtccagc gcctccacgg cgctgcgccc cgcccagatg acgaattgca
     1741 gggcgggaaa atagcgctca caggctgtca gcgccagctg gatcatggct tcgcactggg
     1801 catcgttggc ctcagccccg ccggcgagaa gcaggctgtt gcgataaatg atggatccgt
     1861 cgctcggcca gagatcgaaa tggccatgca actgctgctc gttgatcagc gccagcaggc
     1921 ggcccacctc ctcgcggcgg ggcggcggca ccttcagatc gaaggcggcg gccacctgca
     1981 gcgtttcgag atcctcgcgc cagcacatcg agagcgacag gtcgctccag ccaccctcca
     2041 ggcgcatcag cacttccgat ggcgtcgagc ggtcgaagct ccagtgatgc gcttccgcca
     2101 ggcgctcgat gcggtcgagc ggatttggca ggtcatggtc gatggcctcg aggccgggca
     2161 ttcgcaatcc tccgggggct gacgaatcgg cgccagcatg agtgtgaccg tgcccaaggc
     2221 tttgagtcgc ggcaagggcg gtcgcagcat tgcgcgtgat tcctccacac cgaatcaccg
     2281 cgtagaatct tgcacttgac cagaggagcc gattccctga gcatgccggg cttcactatg
     2341 atcgcagtgt ggcgcgcgca tgaccgaaac gtgtgacttt ctcatcgtcg gcggcggctc
     2401 gtccggatgc atcgtcgcga agcgcctcgc ggagcggacg cgtggccgaa tcattctcct
     2461 cgaagccgga aagccggatg agggtgaccc ggcagccacc gatctcaacc gtctcgacga
     2521 acagaccgcc gactacgact ggggcttcag agccgcgacc cttcgcg
//
LOCUS       DBNGVR010000292         4292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4821026, whole genome shotgun sequence.
ACCESSION   DBNGVR010000292 DBNGVR010000000
VERSION     DBNGVR010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4821026"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /gene="urtC"
                     /locus_tag="AC16A0_06255"
     CDS             <1..707
                     /gene="urtC"
                     /locus_tag="AC16A0_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004123558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HIR3104969.1"
                     /translation="VFGWFAFRSRVTGVYLSIITQALTYALLLAFFRNDMGFGGNNGL
                     TDFKDILGFNIQSQGTRAALFAATALTLAAFLLMLQWIVASRLGKVMVAVRDAESRTR
                     FIGYRVEHVKLFAFTLSAVMAGVAGALYVPQVGIINPGEFSPANSIEIVIWTAVGGRG
                     TLVGPIIGALTVNAGKSYFTAAFPDAWLLVLGALFVCVTLFLPKGIVGTLSEWWEKRP
                     RRNAAKAPVASMEAAQ"
     gene            704..1450
                     /gene="urtD"
                     /locus_tag="AC16A0_06260"
     CDS             704..1450
                     /gene="urtD"
                     /locus_tag="AC16A0_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HIR3104970.1"
                     /translation="MSKQGSILYLDGITVSFDGFKALNSLSLIIGEGEMRAIIGPNGA
                     GKTTMMDVITGKTRPDEGEALFGGDIDLLTRDEATIARLGIGRKFQRPSVFEGHTVWD
                     NIELALEGNRGVVDSLFHRLSPLETARIEEILETVGLTAQARRLAAHLSHGQKQWLEI
                     GMLLAQEPKLLLVDEPVAGMTDDETAATARLLKRIAREKAVVVVEHDMSFVRELGVRV
                     TVLHEGAVLAEGSLDAVSADPRVIEVYLGR"
     gene            1461..2162
                     /gene="urtE"
                     /locus_tag="AC16A0_06265"
     CDS             1461..2162
                     /gene="urtE"
                     /locus_tag="AC16A0_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HIR3104971.1"
                     /translation="MINLSVTGLDVYYGAAQALRGVSLDAKPGSITCVLGRNGVGKSS
                     LLRAIMGLQPVKSGGIAIDGKSIGGLAPYARVASGLGYVPQGREIFPLLTVEENLQTG
                     LTVLPRAERHVDAEIFRLFPVLKTMLARRGGDLSGGQQQQLAIARALVTQPRLLVLDE
                     PTEGIQPSIIKDIGHAIRFLRDTAGMTILLVEQYLDFCRALADHVYIMDRGEIVHSGP
                     AADLDRPEVRRHLMV"
     gene            2345..3301
                     /locus_tag="AC16A0_06270"
     CDS             2345..3301
                     /locus_tag="AC16A0_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HIR3104972.1"
                     /translation="MLPLVGPQPARAAGAIKTAAIYTVPVEQQWVSRIHKAATAAQAR
                     GDIEYVFSEKVSNTDYERVMREYCEAGNQLIVGEAFAVEDAARSVAKDYPDRAFLIGS
                     SFKPDAAANPNFAVFDNYIQDASYLTGIIAGAMTKSGKIGMVGGYPIPEVNRLMNAFM
                     AGVKETRPDATFLVAFIGSWFDPPKAKETALAQIDAGADLLYAERFGVSDAAKEKGVL
                     AVGNVIDTQADYPETVVASAIWHFEPTLDAAIGNIKAGTFKADDYGVYSFMKNGGCSL
                     APLGTFDAKVPAEIKAKVAEKEAAIKSGGFTVTIDDNEPKSS"
     gene            3312..>4292
                     /locus_tag="AC16A0_06275"
     CDS             3312..>4292
                     /locus_tag="AC16A0_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3104973.1"
                     /translation="MTRTATGERDTVLRLDGITKIFGALVANDGISLTLDRGEILGLL
                     GENGAGKTTLMNILFGHYVADAGSIAVEGRPLPPGDPAASLAAGIGMVHQHFTLAENL
                     TVLDNIVLGTESLWRLSSRRDAAHSRIRKLGEDYGLAVDPDATVASLSVGERQRVEIL
                     KALYRDARILILDEPTAVLTPQESAALFETLKRFVASGLSIIFISHKLGEVLAACSRV
                     TILRGGKLVADRDVAGVTRAELAELMVGRPIPEAKSEPRVPGAAVLRLRGLSAARGLL
                     SLRDVDLEIRQGEVVGIAGVAGNGQSLLADVISGMQQPSHGTLELNGTTLH"
BASE COUNT          717 a         1434 c         1418 g          723 t
ORIGIN      
        1 tcgtcttcgg ctggttcgcc ttccgctcgc gcgtcaccgg cgtctatctc tcgatcatca
       61 cccaggcgct gacctatgcg ctgctgctgg ccttcttccg caacgacatg ggcttcggcg
      121 gcaacaatgg cctcaccgac ttcaaggaca ttctcggatt caacatccag tctcagggaa
      181 cccgcgccgc gctcttcgcg gcaacggcgc tgacgcttgc cgccttcctg ctcatgctgc
      241 agtggatcgt ggcctcgcgg ctcggcaagg tcatggtggc ggtgcgcgat gcggaatccc
      301 gcacccgctt catcggctac cgcgtcgaac acgtgaagct cttcgccttc accctgtcag
      361 ccgtcatggc aggcgtggcc ggtgcgctct acgtgccgca ggtgggcatc atcaatccgg
      421 gcgagttctc gcccgccaat tcgatcgaga tcgtgatctg gacggcggtg ggcgggcgcg
      481 gcacgctggt cgggccgatc atcggcgcgc tcaccgtcaa cgccggcaag agctatttca
      541 ccgccgcctt cccggatgcc tggctgctgg tgctgggcgc actcttcgtc tgcgtgaccc
      601 tgttcctgcc caagggcatc gtcggcacgc tctccgagtg gtgggagaag cggccgcggc
      661 gcaatgcggc gaaggcaccg gtcgccagca tggaggcggc gcagtgagca agcagggcag
      721 catcctctat ctcgacggca tcacggtgag cttcgacggc ttcaaggcgc tcaacagcct
      781 gtcgctgatc atcggcgagg gcgagatgcg cgccatcatc ggccccaatg gcgcgggcaa
      841 gacaaccatg atggatgtca tcaccggcaa gacccggccc gacgagggcg aggcgctgtt
      901 cggcggcgac atcgatctgc tgacgcgcga cgaggccacg atcgccaggc tcggcatcgg
      961 ccgcaagttc cagcgcccat cggtcttcga gggtcacacg gtgtgggaca acatcgagtt
     1021 ggcgctggag ggcaaccgcg gcgtggtgga cagcctcttc caccgcctgt ctcccctgga
     1081 aacagcgcgc atcgaggaaa tcctcgagac cgtcggcctc actgcgcagg ccaggcggct
     1141 ggcggcgcat ctcagccatg gccagaagca gtggctggag atcggcatgc tgctggcgca
     1201 ggaaccgaag ctgctgctgg tcgacgaacc ggtggccggc atgaccgacg acgagacggc
     1261 ggcgacggcg cggctgctga agcgcatcgc ccgggagaag gccgtggtcg tggtggagca
     1321 tgacatgtcc ttcgtgcgcg aactgggggt gcgcgtcacc gtgctgcacg agggcgcggt
     1381 gctggccgaa ggctcgctcg atgcggtgag tgccgatccg cgcgtgatcg aagtctatct
     1441 ggggcggtga gcgggcgcgc atgatcaatc tctcggtcac cggactcgat gtctactacg
     1501 gcgcggccca ggcgctgcgt ggcgtgtcgc tggatgccaa gccggggtcc atcacctgcg
     1561 tgctcggccg caacggcgtg ggcaagtcca gcctgctgcg cgccatcatg gggttgcagc
     1621 ctgtgaagag cggcggcatc gccatcgacg gcaaatccat cggcggcctt gcgccctatg
     1681 cgcgcgtggc ctcgggcctc ggctatgtgc cgcagggacg cgagatcttt ccgctgctga
     1741 cagtggagga gaatctgcag accggcctca ccgtgctgcc gcgcgccgag cgccatgtcg
     1801 atgccgagat cttccgcctg tttccggttc tgaagaccat gctggcgcgg cgcggcggcg
     1861 atctttcggg cgggcagcag cagcaacttg ccatcgcgcg cgccctggtg acgcaaccca
     1921 ggctgctggt tctcgacgaa cccaccgagg gcatccagcc ttcgatcatc aaggacatcg
     1981 gccatgccat ccgcttcctg cgcgacacgg cgggcatgac catcttgctg gtcgagcagt
     2041 atctcgactt ctgccgcgcg cttgcagacc acgtctacat catggatcgc ggcgagatcg
     2101 tgcacagcgg cccggctgcc gatctcgaca ggccggaggt acgccggcat ctcatggtgt
     2161 gatgaggcag atcgcggtgg acaacgcttg caaaggcccg tgccgcttgc tggacagaac
     2221 cccgtggcgg gtgccatagt tttgaggaag agctctcgag cgagctcgca gggaggacgc
     2281 aatgacactt cgcaatcttc acgtcacacg ccgcaacgtg ctggccctcg gcgccgccgg
     2341 cgccatgctg ccgctggtcg gtccgcaacc ggcgcgcgcc gccggcgcca tcaagacggc
     2401 cgccatctat accgtgccgg tggagcagca atgggtgagc cgcatccaca aggcggcgac
     2461 cgcggcgcag gcccgcggcg acatcgagta tgtcttctcc gaaaaggtct ccaacaccga
     2521 ctacgagcgc gtcatgcgcg aatactgcga ggcgggaaac cagctcatcg tcggcgaggc
     2581 cttcgccgtc gaggatgcgg cgcgcagcgt ggccaaggat tatcccgacc gcgccttcct
     2641 catcggctcc agcttcaagc ccgatgccgc ggccaatcca aacttcgcgg tcttcgacaa
     2701 ctatatccag gacgcttcct atctcaccgg catcatcgcc ggcgccatga ccaagtcggg
     2761 caagatcggc atggtgggcg gctatccgat ccccgaggtg aaccggctga tgaacgcctt
     2821 catggccggg gtgaaggaga cgcggcccga cgccaccttc ctggtcgcct tcatcggttc
     2881 ctggttcgat ccgcccaagg cgaaggagac tgcgctggcc cagatcgatg ccggcgccga
     2941 cctgctctac gccgagcgct tcggcgtctc ggacgcggcg aaggagaagg gcgttctggc
     3001 ggtgggcaat gtcatcgaca cccaggccga ttatcccgaa acggtggtgg cctcggcgat
     3061 ctggcacttc gagccgacgc tggatgcggc catcggcaac atcaaggccg gtaccttcaa
     3121 ggccgacgac tacggcgtct attccttcat gaagaacggc ggctgcagcc tggcgccgct
     3181 cggcaccttc gacgccaagg tcccggccga gatcaaggcc aaggtcgccg agaaggaagc
     3241 ggccatcaag tcgggcggct tcaccgtgac catcgacgac aacgagccca agtcctcctg
     3301 acgcggcccg catgacccgc acagcaaccg gagaacggga taccgttctc cggcttgacg
     3361 gcatcaccaa gatcttcggc gcgctcgttg ccaatgatgg gatttcgctc acgcttgatc
     3421 gcggcgagat cctcggcctg ctgggcgaga acggcgcggg gaagaccacg ctgatgaaca
     3481 tcctcttcgg ccactacgtg gccgatgcgg ggtccattgc cgtcgagggc cgtccactac
     3541 cgccaggcga tcccgcggcg tctcttgcgg ccggcatcgg catggtgcac cagcacttca
     3601 ccctggccga gaacctgacc gtgctggaca acatcgtgct gggcaccgaa agcctgtggc
     3661 ggctgtcctc gcggcgcgac gcggcgcaca gccgaatccg caagctgggc gaggactacg
     3721 ggctggccgt cgatcccgac gcgaccgtcg cttcgctttc ggtgggcgag cgccagcgcg
     3781 tcgagatcct caaggcgctc taccgcgacg cccgcatcct catcctcgac gaaccgaccg
     3841 cggtgctcac tccgcaggaa tccgcggccc tgttcgagac gctcaagcgc ttcgtggcca
     3901 gcggcctgtc gatcatcttc atttcgcaca agcttggcga ggtcctcgcc gcctgcagcc
     3961 gcgtgacgat cctgcgcggc ggcaagctgg tggcggatcg cgacgtggcg ggcgtcaccc
     4021 gcgccgagct tgcggaactg atggtgggcc ggcccatacc ggaagccaag tcggaaccgc
     4081 gcgtgccggg tgcggccgtt ctccggttga gaggtctttc ggcggcgcgc ggactcctgt
     4141 cgctgcgcga cgttgacctc gagatccggc agggcgaagt ggtcggcatc gccggtgttg
     4201 cgggcaatgg ccagagcctg ctggccgacg tcatctccgg gatgcagcag cccagccacg
     4261 gcacgctgga actgaatggc acgacgcttc ac
//
LOCUS       DBNGVR010000293         3494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2266458, whole genome shotgun sequence.
ACCESSION   DBNGVR010000293 DBNGVR010000000
VERSION     DBNGVR010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2266458"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1422
                     /gene="pstC"
                     /locus_tag="AC16A0_06280"
     CDS             244..1422
                     /gene="pstC"
                     /locus_tag="AC16A0_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR3104974.1"
                     /translation="MLVWTVLIASVLAVGAFLAGQTRASALRVAGGPALHSLPTYHGL
                     FLASGVITGAAVAGLAAILLFGTQSIAVPAAAAIGAAGTGAALYPRIASAFRARNLFE
                     RFVLAVLVLCSAVAVLTTIGIVFSVVFETIIFFRQVSPVEFLFSAQWSPTSTPASFGI
                     LPLLVGTLLITFIAILVAGPLGLLSAIYMAEYASARMRGVLKPLLEMLAGIPTVVLGF
                     FAALTVAPLIRGSGESIGLTVASESALAAGLVMGMMIVPLISSLSDDIINAVPQSLRD
                     GSYAMGATKSETIKRVVLPAALPGIVSAFMLAISRAIGETMIVVMAAGLAANLSVNPL
                     AAVTTITVQIATLLVGDQEFDSPKTLSAFALGFVLFIFTLALNYIALRIVQKYREQYD
                     "
     gene            1415..2890
                     /gene="pstA"
                     /locus_tag="AC16A0_06285"
     CDS             1415..2890
                     /gene="pstA"
                     /locus_tag="AC16A0_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR3104975.1"
                     /translation="MTEVRKGLIAKRYATERRFRIYGATALALTALFIVFLLADIIIK
                     GLPAFTEHRVRLDVTVAADKVDAAKPAAGDYEAIVREALRARFPAVSGRADRRKLNGL
                     LSQGAADDLRQMVVADPALIGKTFPFAALLSDDADLFFKGRQTGVTLAKGSAGLQIAV
                     SGEAYVITGDIAGLSPGQIVLVNEGALRITGIANGAAQAEALARPATLNPAAAGGWNI
                     MTMAKPEADRRISDQEIIWLEELKSEGVVERAFATRFFTSGDSREPELAGLRGALVGS
                     LLTVLVALALSIPLGVAAAIYLEEFAAKNRFTDIIEVNINNLAAVPSIIFGLLGLAMF
                     LSFFGLPRSAPLVGGLVLALLVLPTIIIAARAALKAVPPSIKEAALGVGASHQQAVFH
                     HVLPLALPGILTGIILGLARAFGETAPLLMIGMVAFIVDIPSGFEDAATVLPVQIFLW
                     SDLPEIAFQSKTAAAIMVLLAVLFSLNAVAIYLRRRFERRW"
     gene            2954..>3494
                     /locus_tag="AC16A0_06290"
     CDS             2954..>3494
                     /locus_tag="AC16A0_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR3104976.1"
                     /translation="MAERPVRIEAKDVNIYYGEKHAIKNLSIAIPDRAVSSFIGPSGC
                     GKSTFLRAINRMNDTIPTCRMTGDITLDGSSIYDRDLDVVQLRAKIGMVFQKPNPFPK
                     SIYENVAYGPRIHGISNSKAELDEIVEKSLKRAGLWEEVKDRLNHPGTGLSGGQQQRL
                     CIARAIAVSPEVILMDEPCS"
BASE COUNT          543 a         1239 c         1064 g          648 t
ORIGIN      
        1 ccgagttcgt ctcggagaag gcgctgggcg aggaaggcta tctcgccgag aagggcctcg
       61 tgacgctccc cggcgaccag ctggagaagg tcaaggccac ggtcgatggc atggtggttc
      121 tcaccgcagc cgacgtgaag tgacagcacg cattgccgga gggaccgcgg atgaaccggc
      181 cctccggcaa gacagccggc agcaatgccg gctgacacga ttttcggggc aggggtgtcg
      241 tcaatgctag tgtggaccgt gttgatcgcc agtgtcctcg ccgtcggggc cttcctggcc
      301 ggacagacgc gggcctcggc actgcgggtc gcgggcggcc cggcgctgca ctccctgcca
      361 acctatcacg gcctgttcct cgccagcggc gtgatcaccg gcgcggccgt cgccggactg
      421 gccgccatcc tgctcttcgg cacccagtcg atcgcggttc cggccgccgc cgccattggc
      481 gcagcgggaa ccggcgcggc actctatccg cgcatcgcca gcgccttccg tgcgcgcaac
      541 ctgttcgagc gcttcgtgct tgcggtgctg gtgctctgtt cggccgtcgc cgtgctgacc
      601 accatcggca tcgtcttctc cgtggtcttc gagaccatca tcttcttccg ccaggtctcg
      661 cctgtcgaat tcctgttctc ggcgcaatgg tcgcccacct cgacgccggc gtccttcggc
      721 atcctgcccc tgctggtggg gacactgctc atcaccttca tcgccatcct ggttgccggc
      781 ccgctgggtc tgctctccgc catctacatg gcggaatatg cctccgcgcg catgcgcggc
      841 gtgctgaagc cgctgctcga gatgctggcc ggcatcccga ccgtcgtgct gggcttcttc
      901 gccgcgctca ccgtggcacc cctgatccgg ggcagcggtg aatcgatcgg cctcaccgtc
      961 gcttcggaat ccgccctggc ggcgggcctg gtcatgggca tgatgatcgt gccgctgatc
     1021 tcctcgctct ccgacgatat catcaatgcc gtgccgcagt cgctgcgcga cggttcctac
     1081 gccatgggcg ccaccaagtc cgagaccatc aagagagtgg ttctcccggc agcgcttccg
     1141 ggaatcgtct ctgccttcat gctggccatc tcgcgggcca tcggcgaaac catgatcgtg
     1201 gtcatggccg cagggctcgc cgccaatctc tcggtcaatc cgctggcggc cgtcaccacc
     1261 atcaccgtgc agatcgcaac cctgctggtg ggcgaccagg aattcgactc gcccaagacg
     1321 ctctcggcct tcgcactggg cttcgtcctc ttcattttca ccctcgccct caactacatc
     1381 gcactgcgca tcgtgcagaa gtaccgggaa caatatgact gaggtccgca aaggcctgat
     1441 cgccaagcgc tatgccaccg aacgccgctt ccgcatctat ggcgcgacgg cgctggcgct
     1501 gacggcgctg ttcatcgtct tccttctggc cgacatcatc atcaagggcc tgcccgcctt
     1561 caccgaacac cgcgtgcgct tggacgtgac ggttgccgcc gacaaggtgg atgccgcgaa
     1621 acccgccgcg ggcgactacg aagccattgt gcgcgaggcc ctgcgtgcca ggttcccggc
     1681 ggtcagcggc cgtgccgacc gccgcaagct gaacggcctt ctctcgcagg gcgctgcgga
     1741 cgatctgcgc cagatggtgg tggctgatcc tgcgctcatc ggcaagacct ttcccttcgc
     1801 cgccctgctg tcggacgatg ccgacctctt cttcaagggc cgccagaccg gcgtgacgct
     1861 ggcaaagggc tccgccggtc tgcagatcgc cgtgtcgggt gaggcctatg tcatcaccgg
     1921 cgacatcgcg ggcctgtccc ccgggcagat cgtccttgtc aacgaaggtg cgctgcgcat
     1981 caccggcatc gcgaatgggg cggcccaggc cgaggcgctc gcccggccgg cgaccttgaa
     2041 tcctgccgca gccggcggct ggaacatcat gaccatggcc aagcccgagg ctgaccgccg
     2101 catctcggac caggaaatca tctggctgga agagctgaaa tccgaagggg tggtcgagag
     2161 ggccttcgca acccgcttct tcacctcggg cgactcgcgc gagcccgagc ttgccggcct
     2221 tcgcggcgcc ctggtgggct cgctgctgac ggtgctggtg gcgctggcgc tcagcattcc
     2281 gctgggcgtc gcggcggcta tctatctcga ggaattcgcg gccaagaacc ggttcaccga
     2341 catcatcgag gtcaacatca acaatctcgc ggccgtgccc tcgatcatct tcggcctgct
     2401 gggcctcgcg atgttcctca gcttcttcgg cctgccgcga tccgcgcctc tggtgggcgg
     2461 cctggtgctg gcactgctgg tgctgcccac catcatcatc gcggcccgcg ccgcgctcaa
     2521 ggcggtgccg ccgtcgatca aggaagccgc cctcggcgtc ggggcctcgc accagcaggc
     2581 ggtattccat cacgtcctgc cgctggcact gccgggcatt ctcaccggca tcatcctcgg
     2641 cctcgcccgg gccttcggcg agactgcgcc gctgctgatg atcggcatgg tcgccttcat
     2701 cgtcgacatc ccgagcggct tcgaggacgc ggcaaccgtg cttccggtgc agatcttcct
     2761 gtggtccgac ctgcccgaga tcgccttcca gtccaagacc gccgcagcca tcatggtgct
     2821 gctggcagtc ctcttctccc tgaatgccgt tgccatctac ctgcgccggc gcttcgagcg
     2881 ccgctggtaa aggagtgaac aagacatgga tatgatgccg caagccgtga tcaccccgtc
     2941 gagcccccgc atcatggcgg agcgcccggt acgcatcgag gccaaggacg tgaacatcta
     3001 ttatggcgag aaacacgcca tcaagaatct ctccatcgcc atccccgacc gggcggtctc
     3061 gtccttcatc ggcccgtcgg gctgcggcaa gtccaccttc ctgcgcgcca tcaaccgcat
     3121 gaacgacacg atccccacct gccgcatgac cggcgacatc acgctcgacg gcagcagcat
     3181 ctacgaccgt gacctcgatg tcgtgcagct gcgtgcgaag atcggcatgg tgttccagaa
     3241 gcccaacccg ttccccaagt cgatctacga gaatgtcgcc tatggcccgc gcatccacgg
     3301 catctcgaat tccaaggccg aactcgacga gatcgtcgag aagagcctga agcgtgccgg
     3361 cctgtgggaa gaggtcaagg accgtctcaa tcaccccggc accggcctct ccggtgggca
     3421 acagcagcgg ctctgcattg cccgcgccat tgctgtcagc cccgaggtga tcctgatgga
     3481 cgagccctgt tcgg
//
LOCUS       DBNGVR010000294         1859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3839215, whole genome shotgun sequence.
ACCESSION   DBNGVR010000294 DBNGVR010000000
VERSION     DBNGVR010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3839215"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="AC16A0_06295"
     CDS             <1..541
                     /locus_tag="AC16A0_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3104977.1"
                     /translation="GFEDFGACAAYRVPDAHYDALRGFRHVHIGWINDAGESRRRLRA
                     LGVSVSQDLSVNNRPENLDPDGLSIAFASAAAAAAEAVAAGLLDAGAELAVVTMGADG
                     SLAHDGARSVRMPALPVDPVDTTGAGDSFIAGFLSVHAAGGDLAAALAAGASAARLTC
                     LHPGGFPQQAIDLAGVTIL"
     gene            596..1291
                     /locus_tag="AC16A0_06300"
     CDS             596..1291
                     /locus_tag="AC16A0_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3104978.1"
                     /translation="MLKEIRKEMHGGGGALLDIPWHIRKAFRNFAHPISLRARDILFH
                     AGDAGDGCYIVRRGVVKASVIAKDGQERLLAVLGPGALIGELALIDDEPRSATISAMR
                     DCRLLHLPKSSFFRLADKHPMVYRQALRILAQRLRGTNDTVVAHGTITVAGRVARAFA
                     ALANGLGDELPNGSILLNHKISQTDIAGMAGVARENASRAINELIREGIISRDKSLYV
                     IANPEELLDMATI"
     gene            complement(1296..>1859)
                     /locus_tag="AC16A0_06305"
     CDS             complement(1296..>1859)
                     /locus_tag="AC16A0_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3104979.1"
                     /translation="IDETYKDFRDDPAPPHGIFSKPDWPGAFIHLYSFSKVFSMTGYR
                     VGSIIAGQGFLAEIEKILDCLHICPPHVSQHAALFGLTSLDGWKRDKTSRMRDRLAAL
                     RKAFGRAGLHYELISSGALFAYVRHPFAGQTAKQVAMRLAGEHDLLVLPGSMFGPGQE
                     DYLRVAFANAEVSDMELLVDRLMESQG"
BASE COUNT          318 a          622 c          598 g          321 t
ORIGIN      
        1 cggtttcgag gacttcgggg cctgcgcagc ttaccgggtg cccgacgcgc attacgatgc
       61 cttgcgcggc ttccggcatg tgcacatcgg ctggatcaat gacgcaggcg aatccaggcg
      121 gcggctccgc gcgctgggcg tttccgtgtc gcaggacctt tcggtcaaca atcgaccgga
      181 aaatctcgat cctgacgggc tgagcattgc ctttgcgtcg gccgcggccg cggcggccga
      241 agctgtcgcg gccggtctcc tcgatgcggg cgcggagctg gcggtcgtca ccatgggcgc
      301 cgatggcagt ctggcccatg acggtgcgcg cagcgtccgc atgccggcac tgccggtcga
      361 tccggtcgac accaccgggg caggagacag cttcatcgcc ggcttcctgt cggttcatgc
      421 cgctggcggg gacctcgccg cggccctcgc cgcgggtgca tcggccgcgc gcctcacttg
      481 ccttcacccg ggcggatttc cgcagcaagc cattgatttg gctggcgtca caatcttgtg
      541 atcgatttca catccggttt gtgatatccg tcacagacac aggatcatgg ccagcatgct
      601 gaaggaaatc cgcaaggaga tgcatggcgg gggcggcgcg ctcctcgaca tcccctggca
      661 tattcgcaag gccttccgca atttcgcgca ccccatttcg ctgcgtgccc gcgacatcct
      721 gttccatgcc ggcgatgccg gcgacggctg ctacatcgtg cggcgcggcg tcgtgaaggc
      781 ctcggtcatc gccaaggacg ggcaggaacg cctgcttgcc gtgctgggtc ccggcgcgct
      841 gatcggcgag ctggcgctga tcgacgacga gccgcgctcg gccaccatca gcgccatgcg
      901 cgactgccgc ctgctgcatc tgcccaagtc gtccttcttc cggctcgccg ataagcatcc
      961 gatggtctac cgccaggcgc tccgcattct ggcgcagcgg ctgcgcggca ccaacgacac
     1021 ggttgtggcg cacggcacca tcacggtcgc cggccgcgtg gcccgtgcct tcgcggcgct
     1081 ggccaatggc ctcggcgacg aacttccgaa cggctccatt ctcctgaacc acaagatctc
     1141 gcagacggac atcgccggca tggccggcgt ggcgcgcgag aatgccagcc gcgccatcaa
     1201 cgagctgatc cgcgaaggca tcatcagccg ggacaagagc ctctacgtga tcgccaatcc
     1261 ggaagagctg ctcgacatgg cgacgatctg acgcctcagc cctggctttc catcagccgg
     1321 tcgaccagga gttccatgtc gctgacctcg gcattggcga aggcaacgcg caggtaatcc
     1381 tcctgtcccg gcccgaacat cgatccgggc agcaccagca ggtcatgctc gccggccagc
     1441 cgcatcgcca cctgcttggc cgtctgcccg gcaaagggat gccggacata ggcgaagagc
     1501 gcacccgagc tgatcagttc atagtgcagg cccgcgcggc cgaaggcctt gcgcagcgcc
     1561 gccagccggt cgcgcatgcg ggaagtcttg tcgcgcttcc agccatcgag cgacgtcaga
     1621 ccaaagaggg ctgcatgctg cgacacatgc ggcgggcaga tatgcaggca atcgaggatc
     1681 ttctcgatct cggccaggaa gccctgcccg gcgatgatcg atccgacgcg atagccggtc
     1741 atcgagaaga ccttggagaa actgtagaga tgaatgaagg cgccgggcca gtctggcttc
     1801 gagaaaatgc catggggcgg agcggggtcg tcgcggaaat ccttgtaagt ctcatcaat
//
LOCUS       DBNGVR010000295         3685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1482712, whole genome shotgun sequence.
ACCESSION   DBNGVR010000295 DBNGVR010000000
VERSION     DBNGVR010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1482712"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16A0_06310"
     CDS             complement(<1..242)
                     /locus_tag="AC16A0_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104980.1"
                     /translation="MTRSTALFCMISALLLCLLTPESRAQTLNASDFRIEWKVVNRFR
                     LFADPSFFTLHENAWRQYLLHTDGLDMDADQRAAFVA"
     gene            complement(285..1100)
                     /locus_tag="AC16A0_06315"
     CDS             complement(285..1100)
                     /locus_tag="AC16A0_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR3104981.1"
                     /translation="MSLLNRTVMTEAERHRLMLNVLKDRPFASVRDLLAVVEASPATI
                     RRDINKLHAAGTVRKVFGGIATTELGSGLEPLAARPFEENQMLQVAEKKAIAEAAAAL
                     VRDGDSLIIHGGSTCYLFALLLAQRNLRIFTNSMPMAAALWQKGSCHLTLGGGDLYRE
                     PGILHASGAPPPEFYASKFFLGAQSITHAGMFESHPLIVRETEMLLTRADEVIILADS
                     RKFGFRARYPVMPFSRIDTLITDDRITEANHKMLTDAGVRVIIAKARPETERG"
     gene            1285..2796
                     /locus_tag="AC16A0_06320"
     CDS             1285..2796
                     /locus_tag="AC16A0_06320"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3104982.1"
                     /translation="MTGPAVELRGVSKAFAGIRALTDVSFDVRPGEVHALLGENGAGK
                     STLIKIMAGLHQPDTGEILVNGTARRFASPRDAHAAGIATVHQELLLFPELTVAENIF
                     LGRAPKTSLGTLDWTAMRRRARELLDILDCRELDIDMKVASLSVANRQRIEIARALAQ
                     DARVVIMDEPTAALAEADVQRLMDIVRKLRASGVSIVYVSHRMPEIFALADRVTVLRD
                     GNLIGTMDIAAVDESRLVAMMVGRAVDKLYPARQGQSGATALELRNVSYRTVIRDISF
                     DLRHGEILGLAGLVGSGRTETALTIFGITPATAGEILIDGKPVTITSPEAARDLGIAY
                     VPEDRGSQGLIRAQTIADNVALANLRRLTRGFLMDAAKVLAEARKAIEKFGIRARGPE
                     QIVRQLSGGNQQKVVLGKWLATNPRILIMDEPTRGIDVGAKAEIHLLMRQLAGEGMAI
                     LMISSELPEVLGMSDRVLVMNSGRIVAEFDRAAATPEAVGAAMTHSAASRSAA"
     gene            2793..>3685
                     /locus_tag="AC16A0_06325"
     CDS             2793..>3685
                     /locus_tag="AC16A0_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3104983.1"
                     /translation="MTASAAATPIGRSNLQRFVGGYGQEIVVLLSIVALFVVVGAINP
                     RFLSDTNINSIFAGNAYIAVAAIGMTMLIISGHIDVSVGSLIGVIATIAGTLAVSGYP
                     IWVAWLVPILFGIAVNAGVGALVAYMRIPSIVVTLGMLSILKGGLISATGGEWITDMP
                     QGFMIAQMRLFGIPSAVYFMVILTILAALFMRYTAFGRAIYAVGGNMEAARAAGLDPE
                     RTVVGVFALHGLFAGIAGILFATQLQVIQSTVPPNLELTVITASVIGGVSILGGTGTV
                     IGSTLAAILFACIGSALIFLNV"
BASE COUNT          617 a         1240 c         1192 g          636 t
ORIGIN      
        1 gccacgaagg cggcacgctg gtctgcgtcc atgtcgaggc catcggtgtg cagcagatat
       61 tgccgccagg cgttctcgtg gagcgtgaag aagctagggt ctgcaaagag gcgaaagcgg
      121 ttcaccactt tccactcgat gcggaagtcc gaggcattga gcgtttgggc ccggctctcc
      181 ggcgtaagga ggcagagcag cagcgcggaa atcatgcaga agagagcggt tgacctggtc
      241 atggcgaggc ttctggacgc gaaaccgggc caagtttggg cagatcagcc gcgctccgtc
      301 tccggccgcg ccttggcgat gattacccgg acgccggcgt cggtgagcat cttgtgattg
      361 gcctcggtga tgcggtcatc ggtgatcagc gtgtcgatgc gcgagaaggg catgaccggg
      421 tagcgcgccc ggaaaccgaa cttgcggctg tcggccagga tgatcacctc gtcggcgcgg
      481 gtgagcagca tttcggtctc gcgcacgatc agggggtggg actcgaacat gccggcatgg
      541 gtgatggatt gcgcccccag aaagaatttc gaggcataga attccggcgg aggcgcgccg
      601 ctggcatgga ggatgcccgg ctcgcggtag agatcgccgc ctccaagcgt caggtggcac
      661 gagcccttct gccagagggc cgcggccatc ggcatcgagt tggtgaagat gcgcaggttg
      721 cgctgggcca gcaggagggc gaagagatag caggtgctgc cgccatggat gatgagcgag
      781 tcgccgtcgc gcaccagggc ggcggcggcc tcggcaatgg ccttcttctc ggccacctgc
      841 agcatctggt tttcttcgaa gggccgggcc gccaggggtt ccagcccact gcccagttcg
      901 gtggtggcga tgccgccgaa gaccttgcgc accgtgccgg cggcatgcag cttgttgatg
      961 tcgcggcgga tcgtggccgg ggaggcctcg accacggcaa gcaggtcgcg gaccgaggca
     1021 aagggcctgt ccttgaggac attgagcatc aggcggtggc gttcggcttc ggtcatgacg
     1081 gtccgattga gcagactcaa tcaatgttgc aacgcaaaaa tcacaagata atcaagaact
     1141 aactcacttg acgttctttg ctcatcattg tttattattg ctcaccggct gggtgctgta
     1201 aatgcccggc caaagacctg acccgtgggc gccggatgac cggccaaccg cagggcgtga
     1261 cagcggatgg gagaagactt gcggatgacc ggaccggcgg tcgagttgcg tggcgtctcg
     1321 aaggccttcg ccggaatccg ggcgctgacc gatgtctcct tcgatgtccg ccccggcgaa
     1381 gttcatgccc tgctcggcga gaacggcgcc ggcaagtcca ccctcatcaa gatcatggcc
     1441 ggcctgcacc agcctgacac cggcgagatc ctcgtcaatg gcacggcccg gcggttcgcc
     1501 tcgccgcgcg acgcgcatgc cgcgggcatc gccaccgtgc accaggagct gctgcttttt
     1561 ccggaactga cggtggccga gaacatcttc ctcggccgtg cgccgaaaac gtcgctcggc
     1621 acgctcgact ggaccgccat gcggcgccgc gcccgcgagc tgctcgacat tctcgactgc
     1681 cgcgaactcg acatcgacat gaaggtggca agcctttcgg tggccaaccg ccagcgcatc
     1741 gagatcgccc gcgccctggc gcaggacgcc cgcgtcgtca tcatggacga gcccacggca
     1801 gcgctcgccg aggccgacgt gcagcgcctg atggacatcg tacgcaagct ccgcgcctcc
     1861 ggcgtctcca tcgtctatgt cagccaccgc atgccggaga tcttcgcgct ggccgaccgt
     1921 gtcacggtcc tgcgcgacgg caatctcatc ggcaccatgg acatcgccgc cgtcgacgag
     1981 agccggctgg tcgccatgat ggtgggccgc gccgtggaca agctctatcc cgcccgccag
     2041 ggacaaagcg gcgccaccgc gctggagctg cgcaacgtct cctaccgcac ggtgatccgc
     2101 gacatctcct tcgatctccg ccacggcgaa atcctcggcc tcgcgggtct cgtcggctcg
     2161 gggcgcaccg aaaccgcgct caccatcttc ggcatcaccc cggccaccgc cggcgagatc
     2221 ctgatcgacg gcaagcccgt gaccatcaca agccccgagg cggcgcgcga cctcggcatc
     2281 gcctatgtgc cggaggaccg cggctcgcag ggactgatcc gtgcacagac cattgccgac
     2341 aatgtggcgc tggccaatct gcgccgcctc acgaggggct tcctgatgga tgccgccaag
     2401 gtgctggccg aggcgaggaa ggccatcgag aaattcggca tcagggcccg cgggcccgaa
     2461 cagatcgtgc gccagctctc cggcggcaac cagcagaagg tggtgctggg caagtggctc
     2521 gccaccaatc cccgcatcct catcatggac gagccgaccc gtggcatcga cgtcggcgcc
     2581 aaggccgaga tccacctgtt gatgcgccag ctcgccggcg agggcatggc catcctgatg
     2641 atctccagcg agctgccgga agtgctcggc atgagcgacc gggtgctggt gatgaacagc
     2701 ggccgcatcg tggccgagtt cgacagggcg gccgcgacgc cggaagccgt cggcgccgcc
     2761 atgacgcaca gcgcggcctc ccggagcgcc gcatgaccgc aagcgccgcc gcaacgccca
     2821 tcggacgctc caacctgcag cgcttcgtcg gcggctatgg gcaggagatc gtggtcctgc
     2881 tctccatcgt ggcgctcttc gtggtggttg gcgccatcaa tccgcgcttc ctctcggaca
     2941 ccaacatcaa ttccatcttc gccggcaacg cctatatcgc ggtggccgca atcggcatga
     3001 ccatgctcat catctcgggc cacatcgacg tctcggtcgg ctcgctgatc ggcgtcatcg
     3061 ccaccatcgc gggcacgctc gccgtgtcgg gctatcccat ctgggtcgcc tggctggtgc
     3121 ccatcctctt cggcatcgcg gtcaatgcgg gcgtcggcgc gctggtcgcc tacatgcgca
     3181 ttccctccat cgtggtgacg ctgggaatgc tttcgatcct gaagggcggc ctcatcagcg
     3241 ccacgggagg cgaatggatc accgacatgc cgcagggctt catgatcgcc cagatgcggc
     3301 tcttcggcat cccctcggcc gtctacttca tggtgatcct caccatcctc gccgcgctct
     3361 tcatgcgcta taccgccttc ggccgcgcca tctatgcggt ggggggcaac atggaggccg
     3421 cgcgcgccgc gggcctcgat cccgagcgca cggtcgtcgg cgtcttcgcg ctgcacgggc
     3481 tcttcgccgg catcgccggc attctcttcg ccacccagct gcaggtgatc cagtccaccg
     3541 tgccgcccaa tctcgaactc accgtcatca ccgcctcggt gatcggcggc gtctcgatcc
     3601 tcggcggcac cggcacggtc atcggctcga cgctggccgc cattctcttc gcctgcatcg
     3661 gctcggcgct catcttcctc aacgt
//
LOCUS       DBNGVR010000296         3388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3250267, whole genome shotgun sequence.
ACCESSION   DBNGVR010000296 DBNGVR010000000
VERSION     DBNGVR010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3250267"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="AC16A0_06330"
     CDS             <1..1107
                     /locus_tag="AC16A0_06330"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046866.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3104984.1"
                     /translation="GVEVVRLDAVAFMWKRMGTRCQNEPEVHQLLQALRAASRIVAPA
                     LIHKEEAIVAPKDLIPYLGQGRHTGREGNLAYHNSLMVQFWSALATRETRLMTHVLRT
                     HFPRVFVNATWGTYLRCHDDIGWAITDEDASAVGISGAAHRNFLAEFYAGSFPGSFAR
                     GGDFQPNPGTGDRRSNGSCASLCGLEWAIAAGDPAAIDAAIHRILMGHALIAGFGGIP
                     LIYMGDEIGLTNDHGYESDPNLAHDGRWMHRPMMDWSRAARRREAGSIENRIYTGLRH
                     IIEKRRATPQIHAANPVDILDLDADGIFAFARPSPTGTLVCVFNFAEQWRSIDAARLS
                     QAGVTQWYDTLGEAQIHPQDGSIALPPQGRVWLT"
     gene            complement(1100..2716)
                     /locus_tag="AC16A0_06335"
     CDS             complement(1100..2716)
                     /locus_tag="AC16A0_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3104985.1"
                     /translation="MSIDPISRDVFQHEVVSIAEEMSVALRRSAFSAIIWDMIDYACG
                     LLDPEGNTIAQAPTIPAQLGIMPTAFRHMIRAIPLSEWHEGDVIICNDPYLGCTHTPD
                     LVLFSPVWFEGRIIAITSTVAHHIDVGGRVPGSESATALELYEEGLIIPPLKLFDRGK
                     PNETFFRIFARNVRDPKASTGDINAQVAACRTGERRLKELAEKYGAEGFARHVAAVFD
                     YSETYLRRALRGAVVRAAEAEIFTEDDAASDALMRLKVKADIVDGTLTVDFTGTSDQR
                     PNGLNNPYASTISMVHYAVKVVFTPDLPQNEGCNRPVRVIVPEGSLLNPREPAAVSVR
                     HLTQQAVADVVLKALAQIAPQASSAGCQIAFPTFVIGGFDRRPAMLERTAGVPGYFVV
                     TDIIGGGMGASRDQDGISGVDTHGGNCAILSAEVLETTGPVRVISTRLVEGSGGAGQH
                     SGGLAIEREYEMLSSGLVVSGYTQQTMPETAPWGLEGGEPGGLAAVHLVRRNGLEEKL
                     KSKFVAVPLEEGERLRMRAAGGAGWGRPVK"
     gene            complement(2713..>3388)
                     /locus_tag="AC16A0_06340"
     CDS             complement(2713..>3388)
                     /locus_tag="AC16A0_06340"
                     /inference="COORDINATES: protein motif:HMM:NF039581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104986.1"
                     /translation="DLGMLGVIVPRHPGVFAAEGLLAADIRHLAQVPFRAPLARLDAA
                     ALARTLTQLRETMAHELDADGVDDHRRSMKILGDLRYIGQFHEILVELPDDLLDRLDG
                     HALSELFHRQHEANYGHCDPKAPVEIVNLRIEATGKLDTPNLGSAFSHGAVPAMPERH
                     RMAVMAAGAKAARVPVYARAGLALGQRVAGPAIVTQMDSTTVVLEGQIVEAGAFGVLR
                     IAEAGA"
BASE COUNT          572 a         1168 c         1094 g          554 t
ORIGIN      
        1 ggtgtcgagg ttgtccgtct ggatgccgtg gccttcatgt ggaagcggat gggcacacgc
       61 tgccagaacg agcccgaagt gcatcagctg ctccaggccc tgcgcgccgc cagccggatc
      121 gtggcgccag cgctgatcca caaggaagag gccatcgtgg cgccgaagga cctcatcccc
      181 tatctcggcc agggcaggca cacggggcgc gagggcaacc tcgcctatca caattcgctt
      241 atggtgcagt tctggtcggc gctggcgacg cgcgagaccc ggctgatgac acatgtgctg
      301 cggacccact ttccacgcgt cttcgtcaac gcgacctggg gaacctatct gcgctgccat
      361 gacgacatcg gctgggccat caccgatgag gatgcatcgg ccgtcggcat ctcgggcgcg
      421 gcgcatcgta acttcctcgc cgagttctat gccggaagct ttcccggcag cttcgcccgc
      481 ggcggcgact tccagcccaa tcccggaacc ggtgacaggc gcagcaatgg cagctgtgcc
      541 tcgctctgcg gactcgaatg ggccatcgcc gccggcgatc ccgcggcgat cgacgccgcc
      601 atccaccgca ttctcatggg tcatgcgctg atcgccggct tcggcggcat tccgctcatc
      661 tacatgggcg acgagatcgg cctcaccaac gatcacggct acgaatccga tccgaacctc
      721 gcccacgacg gacgctggat gcacaggccc atgatggatt ggtcccgcgc cgcgcgccgc
      781 cgcgaggccg gcagcatcga gaaccgcatc tacacaggcc tgcgccacat aatcgagaaa
      841 cgccgcgcga caccgcagat ccatgccgcc aatcccgtcg acatcctcga tctcgatgcg
      901 gacggcattt tcgccttcgc gcgtccgagt ccgaccggca ctctggtctg cgttttcaac
      961 ttcgcagaac agtggcgcag catcgatgcc gcgcgcctgt cacaggccgg cgtgacgcag
     1021 tggtacgaca cgctcggcga ggcccaaatt catccgcagg acggcagcat cgcactgccg
     1081 ccgcagggcc gcgtctggct tacttgacag gccttcccca gcccgcgcca cccgcggccc
     1141 gcatgcgcag gcgctcgccc tcttccagcg gcacggccac gaatttgctc ttgagcttct
     1201 cttccagccc gttgcggcgc acgagatgga cggcggcaag tccaccgggt tctccaccct
     1261 caagtcccca gggcgctgtc tcgggcatgg tctgctgcgt gtagcccgat acgacgaggc
     1321 cggacgacag catttcgtat tcgcgctcga tggcaagccc gccgctgtgc tgcccggcgc
     1381 cgccggagcc ctcgaccagc cgcgtcgaga tgacacgaac cggaccggtc gtttccagca
     1441 cctcggccga aagaatggcg cagttgccgc catgcgtgtc caccccgctg atgccgtcct
     1501 ggtcgcgcga tgcgcccatg ccgccgccga tgatgtcggt caccacgaaa tagcccggca
     1561 cgccggcggt gcgctccagc atcgcgggcc tccggtcgaa gccgccgatc acgaaggtcg
     1621 gaaaggcaat ctggcatcct gccgacgagg cctgcggtgc gatctgcgcc agcgccttca
     1681 gcaccacgtc ggccacggcc tgctgggtga ggtgacgaac cgacacggcc gccggctccc
     1741 gcggattgag cagcgacccc tcgggcacga tgacccgcac cggccggttg cagccctcgt
     1801 tctgcggcag gtcgggcgtg aagaccacct tcacggcgta atgcaccatg gagatagtgg
     1861 aggcataggg attgttgagg ccgttgggcc gctggtcgct ggtgccggtg aagtccaccg
     1921 tcagcgtgcc gtcgacgatg tccgccttca ccttcaggcg catcagcgcg tcgctggcgg
     1981 catcgtcctc ggtgaagatc tcggcctcgg ccgcgcgcac cacggcacca cgcagggccc
     2041 tgcgcaggta ggtctcggaa tagtcgaaga ccgccgccac gtgccgggcg aagccctcgg
     2101 cgccgtattt ctccgccagt tccttcagcc gccgctcgcc ggtgcggcaa gccgccacct
     2161 gggcattgat gtcgccggtc gaggccttgg gatcgcggac gttgcgggca aagatccgga
     2221 agaaggtctc gttcggcttg ccgcggtcga agagcttgag gggcgggatg atcaggccct
     2281 cctcgtagag ctccagcgcc gttgccgatt ccgaaccggg cacccggccg cccacgtcga
     2341 tgtgatgcgc cacggtcgag gtgatggcga tgatgcggcc ttcgaaccag accggcgaga
     2401 acagcacgag gtcgggcgtg tgggtgcagc ccagataagg gtcattgcag atgatgacgt
     2461 cgccctcgtg ccactccgac agcgggatgg cgcggatcat gtggcggaag gccgtcggca
     2521 tgataccgag ctgcgccgga atggtcggcg cctgggcgat tgtgttgccc tcgggatcga
     2581 gaaggccgca ggcatagtcg atcatgtccc agatgatggc cgagaaggcc gagcgccgca
     2641 gcgccaccga catctcctcg gcgatcgaga cgacctcgtg ctggaacacg tcgcggctga
     2701 tcgggtcgat gctcatgcgc ctgcctccgc gatcctcagc acgccgaagg caccggcctc
     2761 cacgatctgt ccttccagca ccaccgtggt ggagtccatc tgcgtgacga tggcagggcc
     2821 tgcaacgcgc tgccccaggg cgagacccgc gcgcgcgtag accggcacgc gcgccgcctt
     2881 ggcacctgcc gccatcaccg ccatgcggtg gcgctccggc atcgccggca ctgccccatg
     2941 agagaaggca gaaccaaggt tcggcgtatc cagtttcccg gttgcctcga tccgcaggtt
     3001 gacgatctcg accggcgcct tcggatcgca gtggccataa ttggcctcgt gctggcgatg
     3061 gaagagctcg gacagggcat ggccatccag ccgatccagc aggtcgtccg gcagctccac
     3121 aagaatctca tgaaattggc cgatgtatct gagatcgccc aggattttca tggatctccg
     3181 gtgatcgtcc acgccgtctg cgtcgagttc atgggccatg gtctcgcgca gttgcgtcag
     3241 cgtccgcgcc agcgcagcgg catcgagacg cgcgagcggc gcacggaagg gcacttgcgc
     3301 caggtggcga atgtcggcgg cgagcaagcc ctccgccgcg aagacaccgg gatggcgcgg
     3361 cacgatcacg cccagcatgc cgaggtcg
//
LOCUS       DBNGVR010000297         2399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2072879, whole genome shotgun sequence.
ACCESSION   DBNGVR010000297 DBNGVR010000000
VERSION     DBNGVR010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2072879"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..2100)
                     /locus_tag="AC16A0_06345"
     CDS             complement(82..2100)
                     /locus_tag="AC16A0_06345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3104987.1"
                     /translation="MTITTIKDFPRKIKEVENFWIPLSDGTRLAARMFIPKDAEKNPV
                     PALLEYLPYRKRDGTHVRDALTHPYLAGHGYASIRVDMRGNGDSDGLMADEYTKQEQD
                     DALEVIAWIARQSWCTGKVGMFGISWGGFNALQVAARKPPALKAIVTICSTDDRYEDD
                     VHYKGGTVINEMLGWAATMLAYSANPPDPKLVGNRWRKMWMERLRNEPFLIIPWLSHP
                     HRDAYWKHGSVCEDFGAIEAPTLIVGGWNDAYSNAIPRLMKGLRTTRKAIIGPWVHKY
                     PHFAVPEPRIGFLQEMLRWWDQWLKNEATGVSRDPDFRYYVMEPWKPESFPERINGRW
                     LSDSFWGFGNVDTKKWFLNEKGIGPTAGAETPLTISSKQTTGADGGEYCIIWLGPEFP
                     GDQRRDDEDSLTFETPALITDMDIVGQPAIELSFSVDRPVAHVAVRLNDVWPTGEVSR
                     ITYHLQNLCMRDSRETPAPLEPGKRYRMKIKMDDIAWRVPKGHKIRVSISTSYFPMMW
                     PAPEPVTLTVYAGASQVLLPVRKDILAETPLSWLPAEAAEPVKQKEIRPSSNTRKTVI
                     DDKTGELRIAIVDDFGEQEIKPHGMIVGGTGRETYSILPDDPLSTKMETHWTEIHRRG
                     TWNTRTETYGRLTATKTHWIVWGKIEAFEGKKKVFEKEFNEKIERKLQ"
     gene            complement(2119..>2399)
                     /locus_tag="AC16A0_06350"
     CDS             complement(2119..>2399)
                     /locus_tag="AC16A0_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR3104988.1"
                     /translation="GGGSKYAEPLRETDYAGLPPAFLVAAGHDPLHDDCFDYARNLWN
                     AGVPALVRDEPLLVHAFIRARHMSTPARASFDAIIAACGALAHHGKLT"
BASE COUNT          387 a          770 c          785 g          457 t
ORIGIN      
        1 aaagatgacc cttctcccac ttgcgggaga aggtgcccga agggcggatg agggccgcct
       61 tgcggcgcaa ggcggcggcc gtcactgcag cttccgctcg atcttctcgt tgaactcctt
      121 ctcgaagacc ttcttcttgc cctcgaaggc ctcgatcttg ccccagacga tccagtgcgt
      181 cttggtggcc gtcagccggc cataggtctc ggtgcgcgtg ttccaggtgc cgcggcggtg
      241 gatctcggtc cagtgggttt ccatcttcgt cgaaagcgga tcgtcgggca ggatcgaata
      301 ggtctcacgg cctgtgccgc ccacgatcat gccatgcggc ttgatctcct gttcgccgaa
      361 gtcgtcgacg atcgcgatcc ggagttcgcc ggtcttgtca tcgatcaccg tcttgcgcgt
      421 gttcgacgaa ggccggatct ccttctgctt caccggctcg gcggcttccg cgggcaacca
      481 ggacaggggc gtctccgcca ggatgtcctt gcgtaccggc agcaggacct gcgaggcccc
      541 ggcatagacg gtgagcgtca ccggttcggg cgccggccac atcatcggga agtagctggt
      601 cgagatcgac acgcggatct tgtggccctt cggcacccgc caggcgatgt cgtccatctt
      661 gatcttcatg cggtagcgct tgccgggctc gagcggcgcg ggcgtctcgc ggctgtcgcg
      721 catgcagagg ttctgcaggt gataggtgat gcggctcacc tcgcccgtgg gccagacgtc
      781 gttgagacgc accgccacat gggccaccgg cctgtccacc gagaaagaca gctcgatggc
      841 cggctggccc acgatgtcca tgtcggtgat cagcgccgga gtctcgaagg tgagcgagtc
      901 ctcgtcgtcg cggcgctggt cgccgggaaa ctccgggccc agccagatga tgcagtactc
      961 gccgccatcc gcacccgtgg tctgcttcga gctgatggtg agcggcgtct cggcgccggc
     1021 cgtcggaccg atgcccttct cgttgagaaa ccacttcttg gtgtcgacat tgccgaagcc
     1081 ccagaaactg tccgagagcc agcggccatt gatgcgctcg ggaaagctct ccggcttcca
     1141 cggctccatc acatagtagc ggaagtcggg atcgcggctg accccggtcg cctcgttctt
     1201 cagccactgg tcccaccagc gcagcatttc ctgcaggaag ccgatgcgcg gctccggcac
     1261 ggcgaaatgc ggatacttgt gaacccaggg accgatgatc gccttgcgcg tggtgcgcag
     1321 gcccttcatc aggcgcggaa tggcattcga ataggcgtcg ttccagccgc ccacgatgag
     1381 ggtcggcgcc tcgatcgctc caaagtcctc gcacaccgac ccatgcttcc aataggcatc
     1441 gcgatgcggg tgcgagagcc agggaatgat caggaagggc tcgttcctga gccgctccat
     1501 ccacatcttg cgccacctgt tgccgaccag cttgggatcc ggcgggttcg cggaataggc
     1561 cagcatggtg gcggcccagc ccagcatctc gttgatcacc gtgccgccct tgtaatggac
     1621 gtcgtcctcg tagcggtcgt cggtggaaca gatggtgacg atggccttca gcgccggcgg
     1681 cttgcgtgcc gccacctgca gcgcgttgaa gccgccccac gagatgccga acatgcccac
     1741 cttgccggtg caccagctct gccgggcgat ccaggcgatg acctccagcg cgtcgtcctg
     1801 ctcctgcttc gtgtattcgt ccgccatcag cccgtcgctg tcgccattgc cgcgcatgtc
     1861 gacgcggatc gacgcgtagc catggccggc gagatagggg tgcgtcagcg catcgcgcac
     1921 atgggttccg tcgcgcttgc ggtagggcag gtactcgagc agcgccggaa ccgggttctt
     1981 ctcggcatcc ttcggaatga acatgcgggc cgcaagccgg gtgccatcgg acagcgggat
     2041 ccagaaattc tcgacttcct tgatcttgcg cgggaagtcc ttgatggtcg tgatggtcat
     2101 gaaatcctca ggggtgactt aagtcagttt gccgtgatgg gcgagtgcgc cgcaagcggc
     2161 aatgatggca tcgaaggagg cgcgcgccgg tgtgctcatg tggcgggcgc ggatgaaggc
     2221 atggaccagc agcggctcgt cgcgcacgag ggcgggaacg cccgcgttcc acaggttgcg
     2281 cgcatagtcg aagcagtcgt catgcagagg gtcgtggccg gcggcaacca ggaaggccgg
     2341 cggcagcccc gcataatccg tctcgcgcag cggctcggca tatttgctgc cgccgcccc
//
LOCUS       DBNGVR010000298         2977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3252462, whole genome shotgun sequence.
ACCESSION   DBNGVR010000298 DBNGVR010000000
VERSION     DBNGVR010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3252462"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..987
                     /locus_tag="AC16A0_06355"
     CDS             196..987
                     /locus_tag="AC16A0_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3104989.1"
                     /translation="MTQPDWTDLTRFAVALAQASASEILPFFRRNTAVEVKDGPVWDP
                     VTEGDRAGERIIRRLIEERYPDHGIHGEEYGTKEARSGFTWILDPVDGTRSFVCGMPT
                     WATLIGLSYEGRPVIGLMNQPVVGDMFYGSPEGAWHDYRGAVTAIRTRSGIPLARASI
                     GTTAPELYRSARDQEGFQRLRRAAQRTRYGGDAYFFCVMAAGHLDIALDAGLQAYDIA
                     PLLPIVTGAGGHAAEWTGGDPAKGGHVITAGSEALLHEAMAVLNS"
     gene            1037..2830
                     /gene="ade"
                     /locus_tag="AC16A0_06360"
     CDS             1037..2830
                     /gene="ade"
                     /locus_tag="AC16A0_06360"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR3104990.1"
                     /translation="MTALKSWAESGRRLIDTAMGRVPADMVIRRGRWVNVHSGEVIDD
                     TDIAIADGRFAYVGPDASHCVGRDTVVIAADGRYLVPGLCDGHMHVESGMVTVTEFAR
                     AVIPHGTTSMFIDPHEIANVLGLPGVRLMHDEAAGLPVNVFVQMPSCVPSAPGLETPG
                     AELGPAEVAEAMSWPNIIGLGEMMNFPGVIAGDAKMLAEIAATQRAGKTVGGHYASPD
                     LGLPFHAYAAGGPADDHEGTRAEDAIARVRQGMRAMLRLGSAWYDVAAQVKAITESGL
                     ESRNFILCTDDSHSGTLVNDGHMNRVVRHAIAQGLKPITAIQMATLNTACHFGLEREL
                     GSIAPGRRADLFLTSDLVALPIEMVVARGRVMAEHGRLTAEIPPFAYPASAKNTVKMK
                     RELGADDFAIRAPKGANEVNARVIGVIENQAPTRALEKRLKVSSGLVDAGMDSGVCQI
                     ALVERHKATGGVVNAFVSGFGYNVDCAVASTVAHDSHHMIVVGTNRADMAEAANILRR
                     IGGGAVVVSKGRQLALVELPIAGLMSDERAEIVAAKADQLVAAMKACGCTLNNAYMQH
                     SLLALVVIPELRISDLGLVDVRTFEKIDLFV"
     gene            complement(2831..>2977)
                     /locus_tag="AC16A0_06365"
     CDS             complement(2831..>2977)
                     /locus_tag="AC16A0_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3104991.1"
                     /translation="RIEDGSYGYCEETGEPISLKRLDARPIATLSIEAQERHERREKV
                     YRDD"
BASE COUNT          513 a          986 c          956 g          522 t
ORIGIN      
        1 tttgctcaga cggtcaaatt ctgccgctga gatgcgccgc cacatccacg caaccaactg
       61 aaattaattg tcaaaatgga aaatcttgcg tacctcatgc agtcatgcag tttttgcaat
      121 gatccaaacg agatgttgca acgcacaatt tttcggcact tgagcgggcc ttgcggctga
      181 catacaggac cccggatgac acagcccgac tggaccgacc ttacccgttt cgccgtcgcc
      241 ctggcccagg cctcggccag cgagatcctt cccttcttcc ggcgcaacac ggccgtcgag
      301 gtcaaggatg gtccggtgtg ggacccggtc accgagggcg acagggccgg tgagcgcatt
      361 atccgccgcc tgatcgagga acgctatccc gatcacggca tccatggcga agaatacggg
      421 acgaaggagg cccgttcggg cttcacatgg atccttgacc cggttgacgg gacccgctcc
      481 ttcgtctgcg gcatgcccac ctgggccacg ctgatcggcc tcagttatga aggccggcct
      541 gtgatcgggc tgatgaacca gccggtggtc ggcgacatgt tctatggcag ccccgagggc
      601 gcctggcacg attaccgggg cgcggtgacc gccatccgga cccgcagcgg cattcccctg
      661 gcacgcgcct cgatcggcac cacggcaccc gaactctatc gcagcgcgcg cgaccaggag
      721 ggcttccagc gcctgcgccg tgcggcccag cgtacccgtt acggcggcga cgcatacttc
      781 ttctgcgtca tggcggcggg ccatctcgac attgcgctgg atgccggact gcaggcctat
      841 gatatcgcgc ccctgctccc catcgtgacc ggcgccggcg gccatgccgc cgaatggacg
      901 ggcggcgatc cggccaaggg cggccacgtc atcacggcgg gctccgaagc cttgctccac
      961 gaggccatgg ccgtcctgaa cagctgattg cgcgccgccg cccctcttct atgatggggc
     1021 tttgccggag catgccatga ctgccttgaa aagctgggcc gagagcggcc gccgcctgat
     1081 cgataccgcc atgggccgcg ttcccgccga catggtgatc cgcaggggcc gctgggtgaa
     1141 cgtgcattcc ggcgaggtca tcgacgacac cgacatcgcc atcgcggacg ggcgctttgc
     1201 ctatgtgggc ccggatgcct cccactgcgt cggcagggat acggtggtga tcgcggccga
     1261 tggccgctat ctggtgccgg gcctctgcga cggccacatg cacgtcgaga gcggcatggt
     1321 gacggtgacc gaattcgccc gcgccgtgat cccgcatggc acgacctcga tgttcatcga
     1381 cccgcacgaa atcgccaatg ttctgggact gccgggcgtg cgcctcatgc acgacgaggc
     1441 ggcgggattg ccggtcaatg tcttcgtgca gatgccgagc tgcgtgccca gcgcgccggg
     1501 actcgagacg cccggcgccg aactcggccc cgccgaggtc gcggaggcca tgtcctggcc
     1561 caacatcatc ggccttggcg aaatgatgaa cttcccggga gtgatcgcag gcgatgccaa
     1621 gatgctggcc gagatcgccg ccacgcagcg ggccggcaag acggtcggcg gacactatgc
     1681 ctcccccgat ctcggactgc ccttccacgc ctatgccgcc ggaggcccgg ccgacgatca
     1741 cgaaggaacc cgcgcggaag atgccatcgc ccgggtgcgc cagggcatgc gcgccatgct
     1801 ccggctgggc tcggcgtggt acgatgtcgc cgcccaggtc aaggccatta ctgaatccgg
     1861 tctcgagtcc cgcaatttca ttctgtgtac ggatgactct cactctggca ctctcgtgaa
     1921 tgacggccac atgaaccggg tggtgcgcca tgccattgcc cagggcttga agccgatcac
     1981 cgcaatccag atggcgacgc tcaacacggc ctgccatttc gggctggaac gcgagctggg
     2041 ttccattgcg cccggccgcc gcgccgacct gttcctcacc tcggacctgg tggcactgcc
     2101 catcgaaatg gtcgtcgccc gaggccgcgt catggcggag catggccggc tgacggccga
     2161 gattccgccc tttgcctacc cggcaagcgc aaagaacacg gtgaagatga agcgcgagct
     2221 gggcgccgac gatttcgcca tccgtgcacc gaagggcgcc aacgaggtga atgcccgtgt
     2281 catcggcgtc atcgagaacc aggcgcccac cagggccttg gagaagcggc tcaaggtctc
     2341 ctccgggttg gtggatgccg gcatggactc aggcgtctgc cagatcgcgc tggtcgagcg
     2401 gcacaaggcg accggaggcg tggtcaatgc cttcgtgtcg gggttcggtt acaatgtgga
     2461 ctgcgcggtg gcctcgaccg tcgcccacga cagtcaccac atgatcgtgg tgggcaccaa
     2521 tcgcgccgat atggccgagg cggccaacat cctcaggcgc atcggcggcg gtgcggtggt
     2581 ggtaagcaag ggccgccagc tggcgctggt cgagctgccg atcgccgggc tgatgtcgga
     2641 tgagcgtgcg gagatcgtgg cggccaaggc cgatcagctg gtcgccgcca tgaaggcctg
     2701 cggctgcacg ctcaacaacg cctacatgca gcactcgcta ctggcgctgg tggtaatccc
     2761 cgagcttcgg atctcggacc tcggacttgt cgatgtcaga accttcgaga agattgatct
     2821 cttcgtctga tcaatcgtcg cgatagacct tctcgcggcg ctcatggcgt tcctgcgcct
     2881 cgatcgacag cgtggcgatc ggcctggcgt cgagacgctt gaggctgatc ggctcgcccg
     2941 tctcttcgca atagccgtag gaaccatcct cgatccg
//
LOCUS       DBNGVR010000299         2162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3645117, whole genome shotgun sequence.
ACCESSION   DBNGVR010000299 DBNGVR010000000
VERSION     DBNGVR010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3645117"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1798)
                     /locus_tag="AC16A0_06370"
     CDS             complement(41..1798)
                     /locus_tag="AC16A0_06370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3104992.1"
                     /translation="MTELLDPRLGDVEDDASSTKQRSLMSLAGSLLAEISLPKLLVAW
                     IMLIGLPGLALGFLPLLASLWIGKVSAQVATLLTGLGSLAALILLAAAAWFGGRSIMR
                     LLESSFWSLHAIGIQPGYVVGRESLRHLAESRLKPGGNEAACGRIRAMSALVSAAALS
                     LFAAWIATLAWPHSRWTASLADFAAPLRLALPAIANAVVIVASYFSVASILWGIADAR
                     MAPPLDLTAFDASGSDARRWRIAHLSDVHAVAGPYGFRIESGRSGPRGNHRLAQAFGR
                     LAEIHAQQPLDMILISGDLTDAGSSTEWAAFFDVLGAYPQLSHLIVGLPGNHDLNIVD
                     RANPARLDLPTSPLKRLRQLRMLSALDSLQGGRMRVFDATAGTLGPALDRFLEPHRRS
                     IATFADTGTFRLSRGLGDVFDMAFPMVQPPDHEDGLGIIVLNSNAATHFSFTNALGMV
                     SLAQTQAIDAIAALYPRARWVIALHHHVVEYPSPAKALSERIGTALINGSSLVRRLQN
                     LKGRAMLMHGHRHTEWIGTSGNLIIVSAPSPVMDARDADDTHFFIHTLAAGGDGRIAL
                     LEPEKIVLPGTPAEVSPPA"
     gene            1871..>2162
                     /locus_tag="AC16A0_06375"
     CDS             1871..>2162
                     /locus_tag="AC16A0_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3104993.1"
                     /translation="MTGKAPGSGVESEISTIGGLLLVSTTILVMAALSWGEAVFAPAA
                     FAIFIVMVVWPLQAYLQARMPRMLAILITLVITLVCVVFLLMLFAWGLSVVGQ"
BASE COUNT          396 a          670 c          751 g          345 t
ORIGIN      
        1 aaggcgcaat catttccgtc gacgtgcggc atcgaccgga tcacgccggt ggcgaaacct
       61 ctgcgggcgt gccgggcagc acgatcttct ccggttcgag cagggcgatg cggccatcgc
      121 cgcccgccgc gagcgtgtgg atgaagaaat gggtgtcgtc ggcatcgcgc gcatccatga
      181 ccggcgaggg ggccgacacg atgatgagat tgccgctcgt gccgatccac tcggtatggc
      241 gatggccatg catgagcatg gcgcggccct tcagattctg cagccggcgc accagggagc
      301 tgccattgat cagcgctgtg ccgatgcgtt ccgagagggc cttggccggg ctcggatact
      361 cgaccacatg atgatgcagg gcgatgaccc agcgtgcgcg cggatagagc gcagcgatgg
      421 cgtcgatggc ctgtgtctgc gccagcgaaa ccatgcccag cgcattggtg aaggagaaat
      481 gcgtggcggc attggagttg aggacgatga tgccgagacc atcctcgtga tcgggcggct
      541 gaaccatggg aaaggccatg tcgaagacat cgccgaggcc gcgcgacagg cggaacgtgc
      601 ccgtgtccgc aaaggtggcg atgcttctgc gatgcggctc caggaagcgg tcgagcgccg
      661 ggccgagcgt tccggccgtc gcgtcgaaca cgcgcatgcg cccgccctgc aaagagtcga
      721 gtgccgaaag catgcgtagt tgccgcagcc gtttcagcgg gctggtgggc aggtcaagcc
      781 gcgccggatt ggcgcggtcc acgatgttga gatcgtgatt gccggggagg ccgacgatga
      841 gatgggacag ctgcggatag gcgccaagca cgtcgaagaa ggcggcccat tcggttgatg
      901 agcccgcgtc ggtgaggtcg cctgagatga ggatcatgtc gagcggttgc tgagcgtgga
      961 tctcggccag ccggccgaag gcctgcgcga ggcggtgatt gccgcgcggg cccgagcgcc
     1021 cgctctcgat gcgaaagcca taaggcccgg ccaccgcatg gacgtcggaa agatgggcga
     1081 tgcgccagcg ccgggcgtcc gatcctgatg cgtcgaaggc cgtgagatcg agcggcggcg
     1141 ccatgcgcgc atcggcgatg ccccacagga tcgaggccac gctgaaatag gacgcgacaa
     1201 tcacgacggc attggcgatg gcagggaggg cgagccggag tggcgcggca aagtccgcga
     1261 gactggccgt ccagcggcta tgcggccagg ccagcgtcgc gatccacgcg gcaaacaggc
     1321 tcagggcggc cgcggagacc agcgcgctca tggcgcggat acgcccgcat gcggcttcat
     1381 tcccgcccgg cttcaaccgg ctttcggcga gatggcgcag cgactcgcgg ccgacgacat
     1441 agccaggttg aattccgatc gcatgcaatg accagaagct gctttccagc aatcgcatga
     1501 tcgacctgcc gccgaaccag gccgcggcgg caagcaggat cagcgcggcc agcgagccca
     1561 gtccggtcag cagcgtggcg acctgcgccg agaccttgcc gatccacagc gaggcgagaa
     1621 gcggcaggaa accgagggca aggcccggaa ggccaatcag catgatccag gccaccagga
     1681 gcttgggcag gctgatctcg gccagcaggc tgccggcgag cgacatgaga gatcgctgct
     1741 tggtactgga ggcgtcgtcc tccacgtcac ccagtcgcgg gtcgagcaac tccgtcatgc
     1801 ggcttgccaa atcatgcgcg tatcctcaac atgacagccc tcagaatgcc aaccagacgg
     1861 gaacctcctc atgaccggca aggcccccgg cagcggtgtc gagtccgaga tttccaccat
     1921 aggcggtctg ctgctggtca gcaccacaat actggtcatg gccgccttgt cttggggcga
     1981 ggccgtcttt gcgccggccg ccttcgccat cttcatcgtc atggtcgtct ggccgctgca
     2041 ggcctatctg caggcccgca tgccgcgcat gctggccatc ctcattaccc ttgtgatcac
     2101 cctcgtctgc gtggtgtttc tcctgatgct cttcgcctgg ggcctgagcg tggtggggca
     2161 at
//
LOCUS       DBNGVR010000300         6820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1681104, whole genome shotgun sequence.
ACCESSION   DBNGVR010000300 DBNGVR010000000
VERSION     DBNGVR010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1681104"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC16A0_06380"
     CDS             <1..596
                     /locus_tag="AC16A0_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3104994.1"
                     /translation="NDGIVFLDEIDKICSRSDQGRTGADVSREGVQRDLLPLIEGTTV
                     STKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVELKALGRADFERILTEPE
                     ASLLKQYTALLATEGVTLEFTPDGIAAIAAIAAETNASIENIGARRLQTVMERVLDEV
                     SYAATDRSGETVIVDNAYVEQRVGDLARNADLSKFIL"
     gene            complement(586..963)
                     /locus_tag="AC16A0_06385"
     CDS             complement(586..963)
                     /locus_tag="AC16A0_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3104995.1"
                     /translation="MTPFGDRMRKLRAERNMTLKDMAGAVGVSPAYLSALEHGKRGRP
                     SWHLIQRIIAHFNIIWDEAEEVVHLARISHPRIAIDTAGLSPRATELANRLAAEIGKL
                     DQATLEDIMALVDRKRAKSSVKG"
     gene            complement(960..1535)
                     /locus_tag="AC16A0_06390"
     CDS             complement(960..1535)
                     /locus_tag="AC16A0_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR3104996.1"
                     /translation="MTRKPPDFELWTEVAKSVKPLRAQNRVARSRPALAAAPETHHAA
                     PRLSAPHVHVPRNLPHQPPPITGLDRRTTQKLLRGNVEIERRIDLHGSGIEVARERLY
                     RFLAQCHAEGVRTALVITGKGDSPYARHTLHGAAHFHADERQGRLRRLLADWLHEAQF
                     RSLVSGYQPAHPKHGGGGAFYVRLRRIRSAQ"
     gene            complement(1532..2635)
                     /gene="mltA"
                     /locus_tag="AC16A0_06395"
     CDS             complement(1532..2635)
                     /gene="mltA"
                     /locus_tag="AC16A0_06395"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIR3104997.1"
                     /translation="MAASAGETLHPVTFDDFPGWTEDDHAAALTSFRRSCAEIRDAGR
                     AFQRDIRFGGRRDDWRLACEHASAAMDARVFFEDAFQPFAVRDPQRPEGLFTGYFEPE
                     VDGRHHRERGFETPLYAVPGDLVAFDDAQAALAGVGYGRIVDGQPRPYFTRREIEEGA
                     LAGRGLEILWLRDPADAFFMQIQGSGRVRLADGEIVRLAYGLKSGLPYTGIGHLLAER
                     GAIARSDLSMQSIRAWMAQHPEEARTLMWENRSFVFFREIAAGDPALGPPGAQQVPLT
                     PGRSLAVDRSLWAFGMPVWLDTTKPTPGGEKPLRRLLIAQDTGSAILGLARGDVFFGT
                     GTAAAWSAGHMKSAGRMIVLLPRPLARRLIATP"
     gene            complement(2686..3408)
                     /locus_tag="AC16A0_06400"
     CDS             complement(2686..3408)
                     /locus_tag="AC16A0_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HIR3104998.1"
                     /translation="MAFDPFNLLLLAVAAVVLWRLRSVLGTRTGNERPPFDPFGSKPN
                     KDGPAAEPANGNVLRFPREDGEDAVKPRPDDEPAKPVWHGLAEEGSAVAKGLEEIAGA
                     DRNFAPQSFIEGGKLAYEMIVEAFARGDKQALKPLLSREVFEGFVQAIDQREARGEKV
                     DQRFVGINKASIVGASLKARKARVTLRFVSELISATLSKSGEVIDGDPREIRENTDVW
                     TFERDTTSRDPNWKLVATEASA"
     gene            3525..3992
                     /locus_tag="AC16A0_06405"
     CDS             3525..3992
                     /locus_tag="AC16A0_06405"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIR3104999.1"
                     /translation="MRFFPIIFLAGFFLELASLIWVGSHIGVILTILLVMLAGLAGMT
                     VIRQTGMGIGSALRRAGGRAEVTTLDAGVVFLRMMAGLLLLIPGFVSDAVALVVLLPP
                     VTRWLAQRVFAPVDVQFRASTGERYGGGPVIEGEVVEIDGSLPRPADRKDAEP"
     gene            4223..4645
                     /gene="secB"
                     /locus_tag="AC16A0_06410"
     CDS             4223..4645
                     /gene="secB"
                     /locus_tag="AC16A0_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR3105000.1"
                     /translation="MRILGQYLKDLSFENPNAPKSLAPQSAQPDINISVNVNARNLAP
                     TDYEVELHLDAKATLEGKIVFAAELVYAGAFRLENFPQNLLHPAVLIECPRMLFPFAR
                     QILADATRNGGFPPLMLDPIDFAGMYQKRLQQAPAAQA"
     gene            complement(4642..5334)
                     /gene="dnaQ"
                     /locus_tag="AC16A0_06415"
     CDS             complement(4642..5334)
                     /gene="dnaQ"
                     /locus_tag="AC16A0_06415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR3105001.1"
                     /translation="MREIILDTETTGLEPAAGHRVVEIGAVELFNHVATGKTFHVYIN
                     PERDMPPEAEAVHGLSSAFLKDKPVFEAIAEELMAFIADSVLVIHNASFDMAFINAEL
                     SYLRKPPLPPERVIDTLQIARQKHPTGPNSLDALCRRYGVDNTKRTKHGALLDSELLA
                     EVYLELIGGRQTALSLAAGPAIRRLPSAGAAAVVPRSRVLPPRLSETERAQHRAFIES
                     LGESALWMKSGL"
     gene            complement(5331..5936)
                     /gene="coaE"
                     /locus_tag="AC16A0_06420"
     CDS             complement(5331..5936)
                     /gene="coaE"
                     /locus_tag="AC16A0_06420"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873253.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3105002.1"
                     /translation="MIVIGLTGSIAMGKSETARMFAAEGIPVLDADAEVHRLYDAGGA
                     AVAPLGRLVPEAIRNGKVDRAQLSAAIARDETLLPRIEAIVHPLVRARQQAFLADCRA
                     RGAAIAVLDIPLLFETGRDSEVDSVVVVTAPPEVQRARALARPGMTAEKLELILARQI
                     PDAEKRRRADYLVDSAQGLQAARRQVAAILADIESKRSGAS"
     gene            complement(5933..>6820)
                     /locus_tag="AC16A0_06425"
     CDS             complement(5933..>6820)
                     /locus_tag="AC16A0_06425"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR3105003.1"
                     /translation="PPAAAGFPAQRRRDRIMIKACVIGWPIAHSRSPLIHGYWLKTYG
                     IDGSYTRVPVEPENLRPFIGGLKANGFAGCNVTMPHKEEVFRLVTVADEQTRRLGSLN
                     TVYMENGTLYGTSTDGLGFLANLKSTVPGLDLRNRKVVLLGAGGSAAAIAGNLLAEGV
                     AELCIVNRSLPRAQAIADRFGSGIRPASWDRRAFELAEASLLVNTTSLGMAGQAPLDI
                     SLAALPAGAVVADIVYTPLVTDFLKAAQAAGHPVVTGLGMLLHQAVPGFEKWFGRRPV
                     VTRELYDLTARDIDPTYRP"
BASE COUNT         1187 a         2205 c         2259 g         1169 t
ORIGIN      
        1 aaaacgacgg tatcgttttc ctcgacgaga tcgacaagat ctgctcccgc tccgatcagg
       61 gccgcacagg cgccgacgtt tcgcgcgaag gcgttcagcg cgacctgctc ccgctgatcg
      121 agggcactac ggtgtcgacc aagcacgggg ccgtgaagac cgaccatatc ctcttcatcg
      181 cctcgggtgc tttccacatc gccaagccgt ccgacctgct gcctgagctg cagggccgct
      241 tgccgatccg ggtcgagctg aaggccctgg gccgtgccga tttcgagcgc atcctgaccg
      301 agccggaagc aagcctgttg aagcagtata cggccctgct cgccaccgaa ggcgtcaccc
      361 tcgagttcac gcccgacggc atcgccgcca tcgcggcgat cgccgcagag accaatgcca
      421 gcatcgagaa tatcggggcg cgccgcctgc agacggtcat ggagcgggtg ctcgacgagg
      481 tgagctacgc cgccaccgac cggtcgggcg agaccgtgat cgtcgacaac gcctatgtcg
      541 agcagcgggt gggcgatctg gcgcgcaacg ccgatctttc gaaattcatc ctttgacgga
      601 tgatttcgcg cgcttgcggt cgaccagcgc catgatgtct tccagcgtcg cctgatcgag
      661 ctttccgatc tcggcggcga gccggttggc cagctcggtg gcccgcggcg acaggccggc
      721 ggtgtcgatg gcgatgcgcg gatgcgagat ccgtgccaga tgcaccacct cctcggcctc
      781 gtcccagatg atgttgaagt gggcgatgat gcgctggatc agatgccagc tggggcgccc
      841 gcgcttgcca tgttccagcg ccgagagata ggcgggcgaa acgcccaccg ccccggccat
      901 gtccttgagc gtcatgttgc gctcggcccg aagcttgcgc atacggtcgc cgaagggcgt
      961 cattgggcgc tccggatgcg gcgcaggcgc acatagaagg cgccgccgcc gccatgcttg
     1021 ggatgggccg gctgatagcc ggaaaccagg ctgcggaatt gcgcttcgtg cagccagtcc
     1081 gccagcaggc ggcgcaaccg tccctggcgc tcgtcggcat ggaaatgcgc cgcgccatga
     1141 agcgtatgac gcgcataggg cgaatcaccc ttgccggtga tgaccagcgc cgtgcgcaca
     1201 ccttcggcat gacactgcgc gagaaagcgg tagaggcgtt cgcgcgccac ttcgatgccg
     1261 ctgccatgga gatcgatgcg gcgctcgatc tcgacgttgc ctcgcagaag cttctgggtc
     1321 gtgcgccggt cgagaccggt gatcggcggc ggctggtgcg gaaggttacg cggcacatgc
     1381 acgtggggcg ctgagagtcg cggggcggca tgatgggtct cgggcgctgc cgcaagggcc
     1441 ggacggctgc gggccacgcg gttctgcgcg cgcagcggtt tcaccgactt ggcgacttcg
     1501 gtccagagtt cgaaatcggg gggcttgcgg gtcatggcgt cgcgatcagg cggcgagcca
     1561 gcggcctggg cagaagcacg atcatgcggc cggcgctctt catgtggccg gcactccagg
     1621 cagccgccgt gccggtgccg aagaagacgt cgccacgggc gaggccgaga atcgccgatc
     1681 ccgtatcctg ggcgatgagc agacggcgca atggcttctc tccgccgggc gtcggtttcg
     1741 tggtgtccag ccagaccggc atgccaaagg cccagaggct gcggtcgacg gcgaggctgc
     1801 ggccaggcgt gagaggcacc tgctgcgcgc caggagggcc aagcgccgga tcgccggccg
     1861 cgatctcgcg aaagaagacg aaggaacggt tttcccacat cagggtgcgg gcttcctccg
     1921 gatgctgcgc catccacgcg cgaatcgact gcatcgagag gtcggagcgg gctatcgcgc
     1981 cgcgttcggc caggagatgg ccgatcccag tatagggaag ccccgacttc aggccatagg
     2041 cgaggcggac gatctcgcca tcggcaaggc gaacccgtcc cgagccctgg atctgcatga
     2101 agaaggcatc ggccggatcg cggagccaca ggatctcgag accgcgcccc gctagcgcgc
     2161 cctcctcgat ctcgcggcgg gtgaagtaag gcctgggctg tccgtcgacg atgcggccgt
     2221 aacctacgcc agccaatgcc gcctgcgcat cgtcgaaagc gacaaggtcg ccgggcaccg
     2281 cgtagagtgg cgtctcgaat ccgcgctccc tgtggtggcg tccatccact tcgggttcga
     2341 agtaacccgt gaagaggccc tccggccgct gcggatcgcg aaccgcgaag ggctggaacg
     2401 cgtcttcgaa gaagacgcgc gcatccattg ccgcacttgc atgctcgcag gccaagcgcc
     2461 agtcgtcgcg gcgtccgccg aaccggatat cgcgctggaa ggcgcggcct gcgtcgcgga
     2521 tctcggcaca ggatcgccgg aagctcgtca gggccgctgc atgatcgtct tccgtccaac
     2581 ccggaaaatc gtcgaaggtc acaggatgca gggtctcgcc ggcgctagcc gccattggag
     2641 ccaccatggc tgcaaggagc gcaagcgcaa gggctggccg ccatgtcacg cggacgcttc
     2701 ggtcgccacc agtttccagt tgggatcgcg cgaggtggta tcgcgctcga aggtccagac
     2761 atcggtgttt tcgcggatct cgcggggatc gccgtcgatc acctcgcccg acttcgacag
     2821 cgttgccgat atcagttcgc tgacgaagcg caatgtgacc cgcgccttgc gggctttcag
     2881 gctggcaccg acgatgctgg ccttgttgat gccgacgaaa cgctgatcga ccttttcgcc
     2941 gcgcgcttcg cgctgatcga tggcttggac gaagccctcg aaaacctcgc gcgacagcag
     3001 cggcttgaga gcctgcttgt cgccgcgcgc aaaggcttcg acgatcatct cgtaggccag
     3061 cttgccgcct tcgatgaagc tctggggtgc gaaattcctg tccgctcccg caatctcttc
     3121 caggcccttg gcgaccgcgc ttccctcttc ggcaagcccg tgccagaccg gcttggccgg
     3181 ttcgtcgtcc gggcgcggct tgacggcgtc ctcgccgtcc tcacggggaa agcgcagcac
     3241 attgccattg gcaggttcgg cggcggggcc atccttgttt ggcttggagc caaagggatc
     3301 gaagggcgga cgttcattgc ctgtccgcgt gcccagcacc gaacgaaggc gccacagcac
     3361 gacggcggca acggccagaa ggaggagatt gaacggatca aaagccatcg gttggtcctg
     3421 gtcatgcaat gaaaacctgt tggtgcggcg caacaggctt gcctatatat aggcgagtcc
     3481 gcgtcaggtc caagccgaaa gggccggcgc cagggaaatg catcttgcgc ttctttccga
     3541 tcatcttctt ggccggcttc ttcctcgaac tggccagcct catctgggtg ggcagccata
     3601 ttggcgtgat cctcaccatc ctgcttgtca tgctggccgg tcttgccggc atgaccgtca
     3661 tccgccagac gggcatgggg atcggcagcg ccctgcgccg ggccggcgga cgcgccgagg
     3721 tgactaccct tgatgcgggc gtggtcttcc tgcgcatgat ggccggcctg ctgctgctga
     3781 tcccgggttt cgtgtccgac gctgtggcgc ttgtcgtcct ccttccgccg gtgacgcgct
     3841 ggctggcgca gcgggtcttc gccccggtcg atgtccagtt cagggcctcg acgggcgaac
     3901 gctatggcgg agggccggtg atcgaaggcg aggtggtgga gatcgacggc agcctgccgc
     3961 gtcctgccga ccgcaaggat gccgaaccat agcctgtctt gcgacgcagg ggatggcgtg
     4021 ttagccatgc cgcgctgatc agagaggcca taccatgacg aaagccgcaa aacccgccgc
     4081 aaagaaggct gcacccgctg ccgcaaaggc cgcaccacgc gcgaccaatg gcgaggggcg
     4141 tgccgccaag gtccaggcgg caccgccggc cgcggacatc cagatccagg caccccagcc
     4201 gcagcccggc gcccagcccg ccatgcggat cctgggccag tatctcaagg atctcagctt
     4261 cgagaatccc aatgcgccca agtcgctggc accgcagagc gcgcagcccg acatcaacat
     4321 ctctgtcaac gtcaacgccc gcaacctggc gccgaccgac tatgaggtcg aactgcatct
     4381 cgatgccaaa gcgacgctgg agggcaagat cgtcttcgcg gccgaactcg tctatgcagg
     4441 tgccttccgg ctggagaact tcccccagaa cctgctgcat cccgcggtcc tcatcgaatg
     4501 cccgcgcatg ctgtttccct tcgcccggca gatcctggcg gatgcgacgc gcaatggcgg
     4561 gttcccgccg ctgatgctcg atccgatcga tttcgcaggc atgtatcaga agcgcctgca
     4621 gcaggcaccc gcggcccagg cctaaagccc cgatttcatc cagagcgcgc tttcgcccag
     4681 gctttcgatg aaggcacgat gctgtgcgcg ctcggtctcc gacaggcgcg gcggcaggac
     4741 gcggctgcgc ggtaccactg ctgccgcacc cgccgacggg aggcggcgga tcgccggccc
     4801 agcggcgagc gacagagcag tctggcggcc accgatcagt tcgagataga cctcggccag
     4861 aagttcggag tcgagcagcg cgccgtgctt ggtgcgcttg gtgttgtcca cgccgtagcg
     4921 gcggcacagc gcatcgaggc tgttggggcc ggtgggatgc ttctgccgcg cgatctgcag
     4981 cgtgtcgatc acccgctccg gcggcagcgg cggcttgcgc agataggaga gttccgcatt
     5041 gatgaaggcc atgtcgaagc tggcattgtg aatcaccaga accgaatcgg cgatgaaggc
     5101 catgagttct tcggcgatgg cctcgaagac cggcttgtcc ttcaggaaag ccgatgacag
     5161 gccatgcacc gcttcggcct ccggcggcat gtcgcgctcg ggattgatgt agacgtggaa
     5221 ggtcttgccg gtggcgacat gattgaagag ttcgaccgcc ccgatctcca cgacgcgatg
     5281 gccggcagca ggttccagcc cggtggtttc ggtatcgagg atgatctctc tcatgaggct
     5341 ccgctgcgct tcgattcgat gtctgccaga atagccgcaa cctgtcggcg tgccgcctgc
     5401 aggccctgcg cgctgtccac aagataatcg gcacgtcgcc gcttttccgc gtccgggatc
     5461 tggcgggcca ggatcaattc aagcttttcc gccgtcatgc cgggtcgcgc cagggcccgc
     5521 gcgcgctgca cctccggcgg agccgtcacc accacgacac tgtcaacctc actgtctcgt
     5581 ccggtttcga acagcagtgg aatgtcgagc acggcgatgg ctgcgccccg cgcgcgacaa
     5641 tcagcgagga acgcctgctg acgcgcacgc accagcggat ggacgatcgc ctcgatccgt
     5701 ggaagcaacg tctcgtccct ggcgatcgct gcagagagct gggcgcgatc gaccttgccg
     5761 tttcgaatcg cctccggtac gagcctgccc agcggggcca ctgcggctcc gccggcatcg
     5821 tagagccggt gaacttcggc gtcggcatcc aggacaggaa taccttcagc tgcaaacatg
     5881 cgcgccgtct cgctcttgcc catggcgatg gagccggtga gtccgatgac gatcatggcc
     5941 gataggtcgg atcgatgtcg cgggccgtga ggtcataaag ctcacgggtg accacgggcc
     6001 tgcggccaaa ccatttttcg aagccgggca cggcctgatg cagcagcatg cccagcccgg
     6061 tcacgacggg atgaccggca gcctgcgcgg ccttcaggaa atcggtgacc agcggtgtat
     6121 agacgatatc ggccacgacg gcgcccgccg gcagtgcggc aagactgatg tccagcggag
     6181 cctgaccagc catgccgagc gaggtcgtgt tgaccagaag gctggcttcg gccagttcaa
     6241 aggcacggcg atcccagctc gccggcctga tgccggaacc gaaacgatcg gcgattgcct
     6301 gggcacgcgg cagggaacgg ttgacgatgc agagttctgc aacaccttcg gccaggagat
     6361 tgcccgcgat cgccgctgcc gatccgccgg cccccagcag gacgactttg cgattgcgaa
     6421 ggtccaatcc cgggacagtc gatttcaggt ttgccagaaa gccaaggcca tccgtgcttg
     6481 tcccgtagag cgttccgttc tccatataga cggtgttgag gctgccgagg cgccgggtct
     6541 gttcgtccgc caccgtgacc agtctgaaga cctcttcctt gtgcggcatg gtcacattgc
     6601 agccggcgaa gccattggcc ttgagtcccc cgatgaaggg gcggagattc tcaggttcga
     6661 cggggacgcg ggtgtagctg ccgtcaatgc cataggtctt cagccagtag ccatgaatca
     6721 gcggcgagcg cgaatgggca atgggccagc cgatgacgca ggccttgatc atgatgcgat
     6781 ctcgccttcg ctgcgcagga aatccagcag cggcaggagg
//
LOCUS       DBNGVR010000301         2690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3055986, whole genome shotgun sequence.
ACCESSION   DBNGVR010000301 DBNGVR010000000
VERSION     DBNGVR010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3055986"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..668
                     /locus_tag="AC16A0_06430"
     CDS             21..668
                     /locus_tag="AC16A0_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3105004.1"
                     /translation="MGLVLDQITVRLHGKPLIAAVSLAIAPGEVVTLMGPSGSGKSSL
                     LAFIAGDLGNPFDGGGTIRLNGRILDGLPPEGRRIGRLFQDDLLFPHMTVGENLLFGM
                     PRGAVALRQSKMHMALEQIGLRDFERRPPQSLSGGQRARVALMRSLLAEPDAILLDEP
                     FSRLDSELRAAMRELVFARIAAAGIPGLLVTHDVTDAPPGGRILRIAPDGVLQHA"
     gene            661..1281
                     /locus_tag="AC16A0_06435"
     CDS             661..1281
                     /locus_tag="AC16A0_06435"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3105005.1"
                     /translation="MLDRYALKLLRPAIETTARQAWRIGLTADTATLIGFGFGMAAAG
                     LVASGHVLLAVVPLILNRALDGLDGALARLSRVTDRGAYLDIGLDFVFYAAIPLAFAI
                     SDPAANALAAAVLLAAFLGTGTSFLAFAVIAEKRGLKSTAYPSKSFYYLGGLTEGTET
                     ILCFLAMCLWPAHFASIAMFYAALCCVTTATRLIAGWHMFGADEKR"
     gene            1624..>2690
                     /locus_tag="AC16A0_06440"
     CDS             1624..>2690
                     /locus_tag="AC16A0_06440"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HIR3105006.1"
                     /translation="MAATARILDFLRDRRPDGPCLVVDLEVIAENYRLFARSMPDTRI
                     FYAVKANPAPEILSLLAGLGSCFDTASVPEIEMALNAGATPDRISFGNTIKKERDIAR
                     AHALGESLFAVDSDEEVEKVARAAPGAKVFCRILTDGAGAEWPLSRKFGCEPAMAVGV
                     LLRAHELGLNPYGISFHVGSQQTRLEAWDSALGEAKAIFDAMQKHGIVLQMVNLGGGF
                     PTRYLRQVPGTGSYATAIHEALTKHFGNAIPETIIEPGRGMVGNAGVIKSEVVLVSKK
                     HANDNIRWVYLDIGKFGGLAETMDEAIRYPIVTARDGDAKAPCIIAGPTCDSADVLYE
                     KTPYDLPVTLSVGDEVLIEAAG"
BASE COUNT          442 a          921 c          825 g          502 t
ORIGIN      
        1 gcgcgcctcg ggaggccgcg gtggggctag tcctcgacca gataacggtc aggctgcacg
       61 gcaagccgct gatcgctgcc gtgagccttg ccatcgcgcc gggcgaagtc gtcacgctga
      121 tgggccccag cggcagcggc aagtcttcgc tgcttgcctt catcgcgggc gacctcggga
      181 acccgttcga cggcggcggc accatccgcc tgaacggcag gattctggac ggcctgccgc
      241 cggaaggccg ccgaatcggc cgcctgttcc aggacgacct tctcttcccc cacatgaccg
      301 tcggcgagaa cctgctcttc ggcatgccgc gcggggcagt ggcgcttcgc cagtcgaaga
      361 tgcacatggc tctggaacag attggcttga gggatttcga acgtcgcccg ccgcagtcac
      421 tttcgggcgg acagcgtgcc cgtgtggcct tgatgcgcag cctcctggcg gagccggatg
      481 ccatcctgct ggatgaaccc ttttccaggc tcgacagcga gcttcgggcg gccatgcggg
      541 aactggtctt tgccaggatc gccgcggccg gaattcccgg ccttctcgtc acccatgatg
      601 ttacggatgc gccacccggc gggcgcatcc tgcgcatcgc gccggatgga gttctgcagc
      661 atgcttgacc gttacgccct gaaactgctc cgtccggcga tcgaaaccac ggcacgccag
      721 gcctggcgca tcggattgac ggctgacacg gcaaccttga tcggttttgg cttcggcatg
      781 gctgcggcag gcctcgttgc ctcgggccat gtcctgctcg ccgtcgttcc gttgattctc
      841 aaccgcgcac tcgacggcct tgacggagcg ctggcgcggc tgtcgcgggt gacggaccgc
      901 ggcgcctatc tcgacatcgg cctcgacttt gtcttctacg cggccatccc gctcgccttt
      961 gccatcagcg atccggccgc aaatgcgctg gcggccgcag tgctgctggc ggcctttctc
     1021 ggcacgggca ccagcttcct ggcctttgcc gtcatcgccg agaagcgcgg gctgaagagc
     1081 acggcctatc catcgaagtc cttctactat ctcggcggac tgaccgaggg caccgagacg
     1141 atcctgtgct ttctcgccat gtgcctctgg ccggcccact tcgccagcat cgccatgttc
     1201 tatgcagcgc tctgctgcgt gacgaccgcc acgcggctga tcgcgggctg gcacatgttc
     1261 ggcgcggacg aaaagcgtta accgcgcatt acgcaccacg gagatttttc ttctccaggc
     1321 tgcagaaaaa acttgaatgc gaccgtgatt tgaatcatat caccctcgcc caaagtcgca
     1381 cgtgcctcaa gggacttccc caaagcaacg acggtgcggg ctttttagtc tctgccggct
     1441 cctccaaagg ccggggttac tgagaggcac cactatcatt gccccatgtg cgatcgggtc
     1501 tcccggtcaa tccaaggcga acaggtcagt acgcgcaaag cgttctccac gtctccatcg
     1561 cgtgggctcg cggcgtcgcg ctggcatcgg caaagctcaa cctgttttcc gggagaccgc
     1621 cccatggccg ccaccgctcg catcctcgac ttcctgcgtg accgacgtcc cgatggcccc
     1681 tgcctcgtgg tcgatctcga agtcatcgcc gaaaactatc gcctcttcgc gcgcagcatg
     1741 cccgacaccc gcatcttcta tgcggtgaag gccaatccgg cgcccgagat cctgtcgctg
     1801 ctggccggcc tcggctcgtg cttcgacacc gcctcggtgc cggagatcga gatggcgctg
     1861 aatgccggcg ccacccccga tcgcatctcc ttcgggaaca ccatcaagaa ggagcgcgac
     1921 atcgcgcggg cccatgcgct gggcgaatcg ctcttcgccg tcgattcgga tgaggaggtg
     1981 gaaaaggttg cacgcgcggc cccgggcgcc aaggtcttct gtcgcatcct caccgatggc
     2041 gctggcgccg agtggccgct gtcgcgcaag ttcggctgcg agcccgccat ggcggtaggc
     2101 gtgctgctgc gcgcccatga gctgggcctc aatccctatg gcatctcctt ccatgtcggc
     2161 tcgcagcaga cccgcttgga agcctgggat tcggccctcg gcgaagccaa ggccatcttc
     2221 gatgccatgc agaagcacgg catcgtgctg cagatggtca atctcggcgg cggcttccca
     2281 acccgatacc tgcgccaggt gccgggcacc ggcagctatg ccaccgccat ccacgaggcg
     2341 ctgacgaagc atttcggcaa tgccatcccc gagaccatca tcgagccggg ccgcggcatg
     2401 gtaggaaatg ccggcgtgat caagtcggag gtcgtgctgg tgtcgaagaa gcacgccaat
     2461 gacaatatcc gatgggtcta tctcgacatc ggcaagttcg gcggcctggc cgagaccatg
     2521 gacgaggcga tccgctatcc catcgtcacc gcccgggacg gcgacgccaa ggcgccctgc
     2581 atcatcgcag gcccgacctg cgattccgcc gacgtgctct acgagaagac gccttatgac
     2641 ctgccggtga ctctctccgt cggcgacgag gttctgatcg aagcggccgg
//
LOCUS       DBNGVR010000302         2984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2271013, whole genome shotgun sequence.
ACCESSION   DBNGVR010000302 DBNGVR010000000
VERSION     DBNGVR010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2271013"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1783)
                     /gene="aspS"
                     /locus_tag="AC16A0_06445"
     CDS             complement(5..1783)
                     /gene="aspS"
                     /locus_tag="AC16A0_06445"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR3105007.1"
                     /translation="MHAYRSHTCGQLRPDHAGQSIRLSGWVHRVRDHGGVLFIDLRDH
                     YGVTQVVADPDSPAFKVAEKVRSEWVIRVDGKVRPRPPGTENRDLPTGLVEVFATGIE
                     VLSQAAELPLPVFGDTEYPEDIRLKYRFLDLRRDRIHQNIMKRGAIIDSLRRRMKEQG
                     FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQFKQLIMMSGFDRYFQIAA
                     CFRDEDARADRSPGEFYQLDIEMSFVTQDDVFNAVEPVLRGVFEEFGGGLPVTQKFPH
                     IAYRDAVRWYGSDKPDLRNPIKMQAVTEHFAGAGFKVFAGMIASDPKVEVWAIPAPKG
                     GSRAFCDRMNSYAQQQGQPGLGYVFWREGEEGGAGPIAKNIGPERTDAIRAQLGLGVG
                     DACFFVAGRPEKFYKFAGDARSKVGEELNLTAKDRFEFCWIVDFPMYEWNEEEKKVDF
                     SHNPFSMPQGGLEALETKDPLDILAYQYDIVCNGVELSSGAIRNHRPEVMKKAFEIAG
                     YPESVLLEKFGGMYRAFQYGAPPHGGIAPGVDRIVMLLCNEPNIREVVLFPMNQQAED
                     LLMGAPSEVSPKQLRELHIRLNLPEA"
     gene            complement(1862..2407)
                     /locus_tag="AC16A0_06450"
     CDS             complement(1862..2407)
                     /locus_tag="AC16A0_06450"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3105008.1"
                     /translation="MLALAGLLAPAAATAATPPKKKAPAKKPLVITINKVSQKMTVSL
                     DGRTKYKWPVSTGAPGYDTPSGTFRPFRMEKEHYSSEWDDAPMPHSIFFTARGHAVHG
                     SYHVKSLGRRASHGCVRLHPDNAAKLFALVKQTGMSNTQVVVKGGFFDTDFIDDDLPL
                     LKKKSTKKPGKRKLFPWELGT"
     gene            2551..>2984
                     /locus_tag="AC16A0_06455"
     CDS             2551..>2984
                     /locus_tag="AC16A0_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR3105009.1"
                     /translation="MNKHVPKVGLVSLGCPKALVDSERIVTQLRAEGYVIAPGYDDAD
                     VVIVNTCGFLDSAKAESLAAIGEAMTENGRVIVTGCYGVEADAIRAAHPGVLAVTGPH
                     QYEAVVGAVHEALPPLHDPRFDLVPPQGLHLTPRHYAYLKIS"
BASE COUNT          512 a          957 c          973 g          542 t
ORIGIN      
        1 tcactcacgc ctccggcagg ttcaaccgga tgtgcagttc gcgcagctgc ttgggcgaca
       61 cctccgaagg agcgcccatc aggaggtctt cggcctgctg gttcatcggg aagagcacca
      121 cctcgcggat attgggctcg ttgcacagca gcatgacgat gcggtccacg cccggcgcga
      181 tgccgccatg gggcggtgcg ccgtactgga aggcgcggta catgccgccg aacttctcca
      241 ggagcacgct ctcaggatat cccgcgatct cgaaggcctt cttcatcacc tcggggcgat
      301 ggttgcggat ggcgccggag gacagctcca cgccgttgca gacgatgtcg tactgatagg
      361 cgaggatgtc gagcgggtcc ttcgtctcca gcgcctcgag gccgccctgc ggcatggaga
      421 agggattgtg cgagaagtcg accttcttct cttcctcgtt ccattcgtac atcgggaaat
      481 cgacgatcca gcagaattcg aagcgatcct tggcggtgag gttcagttcc tcgcccacct
      541 tgctgcgggc atcgccggcg aacttgtaga acttctcggg ccgcccggcg acgaagaagc
      601 aggcatcgcc cacgccaagg cccagctggg cgcggatcgc atccgtgcgc tcgggtccga
      661 tgttcttggc aatcggtccg gcgccgccct cctcgccctc gcgccagaag acataaccga
      721 gaccaggctg tccctgctgc tgcgcataag agttcatgcg gtcgcagaag gcgcgggagc
      781 cgcccttggg cgccgggatg gcccagactt cgaccttcgg atcgctggcg atcatgccgg
      841 cgaagacctt gaagcccgcg cccgcgaaat gctcggtcac ggcctgcatc ttgatggggt
      901 tgcgcaggtc cggcttgtcg gacccatacc agcggaccgc atcgcgatag gcgatgtgcg
      961 ggaacttctg cgtcaccggc agaccgccgc cgaactcctc gaagacgccg cgcagcaccg
     1021 gctccaccgc gttgaacacg tcgtcctgcg tcacgaagct catttcgatg tcgagctgat
     1081 agaactcgcc gggcgaccgg tcggcacggg cgtcctcgtc gcggaagcag gccgcgatct
     1141 ggaaatagcg gtcgaagccc gacatcatga tcagctgctt gaactgctgc ggtgcctgag
     1201 gcagcgcata gaacttgccg ggatggatgc ggctcggcac caggaagtcg cgcgcgcctt
     1261 ccggcgatga tgccgtcagg atcggcgtct ggaactcgaa gaagccctgc tccttcatgc
     1321 gccgccggag cgagtcgatg atggcgccgc gcttcatgat gttctggtgg atgcggtcgc
     1381 ggcgcagatc gaggaagcgg tacttgagcc ggatgtcttc cgggtactcc gtgtcgccga
     1441 agaccggcag cggcagctcg gcggcctggc tcagcacctc gatgccggtg gcgaagactt
     1501 ccacgagtcc ggtcggcagg tccctgttct cggtgccggg cgggcgcggc cgcaccttgc
     1561 cgtcaacccg gatcacccat tcggaccgga ctttctcggc caccttgaag gcgggcgagt
     1621 cgggatcggc caccacctgc gtcacgccgt agtggtcgcg caggtcgatg aagagcacgc
     1681 cgccatggtc gcggacccgg tgcacccagc cggacagccg gatggactgg ccggcatggt
     1741 cagggcggag ctggccacag gtatggctgc gataggcgtg catggaacct ctcaggaacg
     1801 ctggatttgc gcgctagagc gcatgcagag gccgatttgt caagcagccc gggggcttgc
     1861 gtcaggtccc cagttcccag gggaaaagtt tgcgcttccc gggcttcttg gtggacttct
     1921 tcttgagcag cggcaggtcg tcatcgatga agtcggtgtc gaagaaaccg cccttcacca
     1981 cgacctgggt attgctcatg ccggtctgct tgaccagcgc gaaaagcttc gcggcattgt
     2041 ccggatgcag gcgcacgcag ccatgcgagg cccggcggcc caggctcttc acatgatagc
     2101 tgccatgcac ggcgtgtccg cgtgccgtga agaagatcga atgcggcatc ggcgcatcgt
     2161 cccattcgct ggagtagtgc tccttctcca tccggaaggg gcggaaggtt ccgctcggcg
     2221 tgtcatagcc cggtgccccg gtcgacacgg gccacttgta cttggtcctg ccgtccagcg
     2281 agacggtcat cttctggctg accttgttga tggtgatgac cagcggcttc ttggccggcg
     2341 ccttcttttt gggaggagtt gccgccgtgg cggcggcggg tgccagcagg cccgccaaag
     2401 ccagcaagag agaacgcctg gtcgacatca tgcacctcaa aaccaatgga aatcgctacc
     2461 ccaggtcgaa atatgcaaca aacgtccggc gcggggaagt cacttttgga gtgatttgcg
     2521 ctgcggtgcc cgctcggcta aagggcgccc atgaacaagc atgtccccaa agtcggcctc
     2581 gtgtccctcg gttgccccaa ggccctggtc gacagcgaac gcatcgtcac ccagctccgg
     2641 gccgagggtt atgtgattgc gcccggctat gacgacgcgg atgtggtgat cgtcaacacc
     2701 tgcggcttcc tcgacagcgc caaggccgaa tcgctggccg ccatcggcga ggccatgaca
     2761 gagaacgggc gcgtcatcgt caccggctgc tacggcgtcg aggccgatgc catccgcgcc
     2821 gcccatccgg gcgtgctcgc ggtaaccggc ccgcaccagt acgaggcggt cgtcggtgcc
     2881 gtgcacgagg cgcttccgcc gctgcatgac ccgcgcttcg atctggtgcc gccgcagggc
     2941 ctgcatctga cgccacggca ctatgcctat ctcaagattt ccga
//
LOCUS       DBNGVR010000303         3891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5023101, whole genome shotgun sequence.
ACCESSION   DBNGVR010000303 DBNGVR010000000
VERSION     DBNGVR010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5023101"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="AC16A0_06460"
     CDS             complement(<1..628)
                     /locus_tag="AC16A0_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003575022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3105010.1"
                     /translation="MTAFRRLAQGGRIDRAKPVTARFDGKPLMGFAGDTLAAALLAND
                     RIVIGRSFKYHRPRGILSAGIEEPNALFTLGEGARREPNVAATLTDLVDGLVARSQNA
                     WPSPDFDLMAVNSALAPLFQSGFYYKTFMGPTKGAWMFYEPFIRKAAGLGRATALPDP
                     DRYDTRHGFCDVLVVGSGPAGLAAALAAARSGARVVLAEQDLELGGSLL"
     gene            complement(625..867)
                     /locus_tag="AC16A0_06465"
     CDS             complement(625..867)
                     /locus_tag="AC16A0_06465"
                     /EC_number="1.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HIR3105011.1"
                     /translation="MMIPCPYCGNRDHSEFRYGGDASKTRPAHGADDPKTWHDYYFLF
                     DNPKDPHTDYWQHVLGCRRWFRLNRNTATNEIGGPA"
     gene            complement(864..2123)
                     /locus_tag="AC16A0_06470"
     CDS             complement(864..2123)
                     /locus_tag="AC16A0_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HIR3105012.1"
                     /translation="MSDRYSALALARHALQPDLPWPRAWASPEPRTTYDAVIIGGGGH
                     ALATAYYLARNHGLTNVAVLERSYIGSGNVGRNTTLIRSNYVIDGNTQFFEHSLKLWE
                     GLSHDLNFNCMVSQRGQIVLAVSPGQLDTFARRGNIMRLNGIDAELLDRSEVMKLLPD
                     LDYSKEARWPIWGGILQRRAGTVRHDAVAWGYARAASQRGVHIIENCEVTGFVIENGQ
                     AIGVETTRGRIAAGKIGIAVAGHTSHVARMAGLRLPVESHILQAFVTEPVKPIVDHVV
                     SWGAELFYLSQSDKGGLVFGGHIDGFNTYTQRGQFPKIRTVAECAVSLMPFISRLRLL
                     RHWGGIQDMTPDGSPFICRTPVKNLYLNGGWCYQGFKATPASGWTFAHTMAHDEEHEL
                     NRCYSLDRFEKGGERDDYGIGNWTYKQ"
     gene            complement(2172..3368)
                     /locus_tag="AC16A0_06475"
     CDS             complement(2172..3368)
                     /locus_tag="AC16A0_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR3105013.1"
                     /translation="MTGKSQSNIEFILLVALLNAMVAMSIDTMLPAIGTIAAELGATD
                     PNSRQFIITSFFGGMTIGTLVYGPLSDSIGRKPAIYAGLALYAVGSLMCLAAQSFEVM
                     LLGRFVQGFGAAAPRIVSIAMVRDGSAGAGMARVMSFVMTVFMLVPILAPSVGQLVLF
                     FASWRVIFLGFLVMGVIAGVWLWLRQPETLPHSRRSPLSPRALAAAAGEVFRNPVTIG
                     YTIAVGCIFGSFICYLGTSQQLFAEQYNQGAYFAVWFGVFATGIAMAMLLNARLVMRY
                     GMRKLSKWALRGFIVLSLFFLAAGLLMDGHPPLWILALYLFLNFFCSGILFGNYNAMA
                     LEPIGRIAGMAAAISGSMSSLIAIVTGTLIGQQYDGTVIVLAAGFSVLGLAAFVMTEW
                     AERRRA"
     gene            complement(3365..>3891)
                     /locus_tag="AC16A0_06480"
     CDS             complement(3365..>3891)
                     /locus_tag="AC16A0_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HIR3105014.1"
                     /translation="LDASFDAVMMIGYHDAAGSEANSLAHTLSSDAVEIRLNGRRASE
                     FSVHALASSMLGVPTVFVSGDRGLMDHVAEVNPAIGRCAVKQGRGQSTVSMTPAAACK
                     AIRTGAEAALRGDLRACLLPVPAHSVLEITYGNPVLAYRHSWYPGARHAGDRTIRYET
                     DDYFDILRMLNYVT"
BASE COUNT          742 a         1205 c         1312 g          632 t
ORIGIN      
        1 acaggaggct gccgccgagc tcgagatcct gctccgccag cacgacgcgg gcgccgctgc
       61 gcgcagccgc gagcgcagcc gcaagaccgg cggggcctga ccccaccacc agcacgtcgc
      121 agaagccatg gcgggtgtcg tagcggtccg gatccggcaa ggcggtcgcg cgtccaagtc
      181 cagccgcctt gcggatgaag ggttcgtaga acatccaggc gcccttggtc gggcccatga
      241 aggtcttgta gtagaatcca gactggaaga gcggcgccaa tgcggagttc accgccatga
      301 gatcgaagtc cggtgacggc caggcgttct ggctgcgtgc gacgaggcca tcgaccagat
      361 cggtcagcgt ggccgcgaca tttggttctc tgcgcgcgcc ctcgcccagc gtgaagagcg
      421 cgttgggctc ttcgatacca gccgagagaa tgccgcgcgg ccggtgatac ttgaagctcc
      481 ggccgatgac gatgcggtca ttggccaaca acgccgcggc aagcgtatcg ccggcaaagc
      541 ccatgagagg ctttccatcg aagcgcgccg tgaccggttt tgcgcggtcg atgcggccac
      601 cctgggcgag acgccggaac gccgtcatgc cgggcctccg atctcgttgg tggcggtgtt
      661 gcggttcagc ctgaaccagc ggcggcagcc caggacatgc tgccagtagt ccgtgtgcgg
      721 atctttggga ttgtcgaaga ggaagtagta atcgtgccac gtcttcggat catccgcgcc
      781 atgcgcaggc cgcgttttcg aggcatcgcc gccgtaacgg aattccgaat ggtcgcgatt
      841 gccgcaatag gggcacggga tcatcattgc ttgtaggtcc agttgccgat tccgtagtcg
      901 tcgcgttcgc cgcccttttc gaagcggtcg agggaatagc agcggttcag ctcatgctct
      961 tcgtcatggg ccatggtatg cgcgaaggtc cagcccgagg ccggcgtggc cttgaagccc
     1021 tgatagcacc agccgccgtt gagatagagg ttcttcaccg gcgtgcggca gatgaaggga
     1081 ctgccgtcgg gcgtcatgtc ctggatgccg ccccagtggc gcagcaatcg caggcgcgag
     1141 atgaagggca tcagcgacac ggcgcattcg gccaccgtcc ggatcttcgg gaactggccg
     1201 cgctgggtat aggtgttgaa gccatcgata tggccgccaa aaacgagccc gcccttgtcc
     1261 gactgcgaca gatagaagag ctccgcgccc caggacacga catggtccac gatcggcttg
     1321 accggctcgg tcacgaaggc ctgcaggatg tggctctcca ccggcagcct gaggccggcc
     1381 atgcgggcca catgggaggt gtgacccgcc actgcgatac cgatcttgcc cgccgcgatg
     1441 cggccgcgag tcgtctccac gccgatggcc tggccgttct cgatcacaaa gcccgtcacc
     1501 tcgcagttct cgatgatgtg gacgccgcgc tgcgaggcgg cccgcgcgta gccccaggcc
     1561 acggcgtcat gccgcaccgt accggcgcgg cgctgcagga tgccgcccca gatcggccag
     1621 cgcgcctcct tcgagtagtc aaggtcggga agcagcttca tcacctcgct gcggtcgagc
     1681 agctcggcat cgatcccgtt cagccgcatg atgttgccgc gccgcgcgaa ggtgtcgagc
     1741 tgtccggggc tgaccgccag cacgatctgg ccgcgctgcg agaccatgca gttgaagttc
     1801 aggtcgtgcg acaggccctc ccacagcttg agcgaatgct cgaagaactg cgtgttcccg
     1861 tcgatcacgt agttcgagcg gatgagcgtg gtgttgcggc cgacattacc cgaaccaatg
     1921 tagctgcgct cgagcacggc gacgttggtg aggccgtggt tcctggcgag gtagtaggct
     1981 gtggcgagcg cgtggccgcc gccgccgatg atgacggcgt cataggtcgt cctcggctcg
     2041 ggcgaggccc aggcgcgcgg ccagggcaga tccggctgca gggcgtggcg ggcaagggca
     2101 agggcggaat aacggtctga cacaagggtt tgctaacagg tttgggcacc gcccggcaag
     2161 gtgcggaaat ttcacgccct gcggcgttct gcccattccg tcatgacaaa ggcggcaaga
     2221 ccgaggacgg agaacccggc ggcgaggacg atcaccgttc catcatattg ctggccgatc
     2281 agcgtgccgg tcacgatggc aatcagcgac gacatggatc ccgaaatcgc cgccgccatg
     2341 ccggcgatgc ggccaatggg ctccaacgcc atggcattgt agttgccgaa caggatgccg
     2401 ctgcagaaga agttcaggaa cagatagagc gcgaggatcc acagcggcgg gtgtccgtcc
     2461 atgagaaggc ctgcagcaag aaagaacaga gacaagacga tgaagccgcg cagcgcccat
     2521 ttcgacagct tgcgcatgcc gtagcgcatc accagccgcg cattcagcag catggccatc
     2581 gcgatcccgg tggcgaagac gccgaaccag acggcaaaat aggcgccctg gttgtactgc
     2641 tcggcaaaga gctgctgtga ggttccgaga tagcagatga agctgccgaa gatgcagccg
     2701 accgcgatcg tgtagccgat cgtgacggga ttgcggaaca cctcgccggc tgcggcggcg
     2761 agcgccctgg gcgatagcgg agaccgccgg ctgtgtggca gggtttcggg ttgacgcagc
     2821 cagagccaga cgcccgcgat gacgcccatg acaaggaatc cgaggaagat gactcgccag
     2881 ctggcgaaga agagtaccag ctggcccacc gacggcgcaa gaatgggcac cagcatgaag
     2941 accgtcatga cgaaggacat gacccgcgcc atgccggcgc cggcactgcc gtcgcgcacc
     3001 atggcgatcg agacgatgcg gggagcggca gccccgaagc cctggacgaa gcggccgagc
     3061 agcatgactt cgaagctttg cgctgcgagg cacatgagcg agccgaccgc gtagagcgcg
     3121 aggcccgcgt agatcgccgg cttgcggccg atggagtcgg acaatggtcc atagaccagc
     3181 gtcccgatgg tcatgccgcc aaagaagctg gtgatgatga actgccggct gttgggatcg
     3241 gtggcgccga gctctgccgc aatggtgccg atggccggca gcatggtgtc gatcgacatc
     3301 gccaccatgg cgttgagcag cgccacaagc aggatgaact cgatgttgct ttggctcttc
     3361 ccggtcatgt cacgtagttc agcatcctga ggatgtcgaa atagtcgtcg gtttcgtaac
     3421 gaatagtcct gtcgccggcg tgccgtgcgc ccgggtacca gctgtgacga taggcgagga
     3481 ccggattgcc gtaggtgatt tcaagaaccg aatgcgccgg aacgggcagg aggcaggccc
     3541 tgaggtcgcc cctgagcgcc gcttccgcac cggtccggat cgccttgcag gcggcggcgg
     3601 gcgtcatcga gacggtcgac tgtccccgcc cctgcttgac agcgcagcgg ccgatggccg
     3661 ggttgacctc ggccacatga tccataaggc cccggtcgcc ggagacgaac accgtgggca
     3721 cgcccagcat cgacgacgcc agcgcgtgaa ccgagaactc cgacgcccgc ctgccattga
     3781 ggcggatctc gaccgcatcg ctggacaggg tatgtgccag gctattcgcc tcggagcctg
     3841 cggcatcatg atagccgatc atcatgaccg catcgaaact cgcgtcgagt t
//
LOCUS       DBNGVR010000304         2018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2666257, whole genome shotgun sequence.
ACCESSION   DBNGVR010000304 DBNGVR010000000
VERSION     DBNGVR010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2666257"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="AC16A0_06485"
     CDS             <1..815
                     /locus_tag="AC16A0_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3105015.1"
                     /translation="PEMIARLRRQLSNIAVQEYLDQIENRFRAIAALRHRDGIMGDRQ
                     LMHRLYHPEEPVFVHNPSGKGGLMLALATMFNNFQVSNAIFAAVLLELGHSVLILKDC
                     TDFGYLKGVPGLGTDPEALARWVEGFMARQGFEQLHIAGFSSSGFAALLVSAMLRCDS
                     CICFSPLTDLAAGSLLPQPGYLTQALRQQIDARFLCNLATRFEKGKLVTPRTIVFGDQ
                     SAADGRHARNLLGLPGMQLVEVENCGHQTPGTLLERGRLLDVLRQAGAPAAM"
     gene            912..1688
                     /locus_tag="AC16A0_06490"
     CDS             912..1688
                     /locus_tag="AC16A0_06490"
                     /inference="COORDINATES: protein motif:HMM:NF018420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HIR3105016.1"
                     /translation="MKSRALYNRFLSLSATALALWTFWLPAEAEARCLDVALVLAIDG
                     SSSVDSAEYQLQKQGIAEALRDPQVIAAMRQAGDVAVSVIFWGDAGQPHQKIDWFRIA
                     DSKDGDRLAQRIERMHRNVKGSTGLGTGLVAALDMIADADFCANRAIINVSGDGSETV
                     YRRQTRHLPKPEDARARAEEAGVTINALAISNQEGGLKDYFASQVVTGPGAFVMEVKN
                     YESFAEAMRRKLIREITPRAVSGLRLPVAWSDPPRAPDRP"
     gene            1777..>2018
                     /locus_tag="AC16A0_06495"
     CDS             1777..>2018
                     /locus_tag="AC16A0_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3105017.1"
                     /translation="MATPIMNVMIGAVRKAARSLQRDYGELSNLQVSQKAPGDYVTAA
                     DKKCDKILREELQKARPDYTFLTEESGTIKGSDPDHR"
BASE COUNT          383 a          663 c          620 g          352 t
ORIGIN      
        1 caccagagat gattgccagg ctgcggaggc agctcagcaa cattgcggtc caggagtatc
       61 tcgaccagat cgagaaccgg tttcgcgcca tcgcggcact gcgccaccgt gacggcatca
      121 tgggggacag gcagctcatg caccggctct atcatccgga agagccggtc ttcgtccaca
      181 atccgtcggg caaaggcggc ctcatgctgg cgctcgccac aatgttcaac aattttcagg
      241 tctccaacgc catattcgcg gcggtgctgc tggagcttgg acattcggtg ctcattctca
      301 aggactgcac cgacttcggt tacctgaagg gggtgcccgg actcggtacg gatccggagg
      361 cgctcgcgag gtgggtggaa ggcttcatgg cgcggcaagg atttgaacag ctgcacattg
      421 caggattctc ctccagcggc tttgcggccc tcctcgtctc cgcaatgctg cgctgcgaca
      481 gctgcatctg cttctcgccg ctgacggatc tcgccgccgg atcccttctt ccccagcccg
      541 gctacctgac gcaggccctg cgccagcaga tcgacgcgcg ttttctctgc aatctggcca
      601 cgcgcttcga gaagggaaag ctggtcacgc cccggaccat cgtcttcggc gaccagagtg
      661 ccgccgatgg caggcatgcc cgaaatctcc tgggcttgcc ggggatgcag cttgtcgagg
      721 tggagaactg cggacaccag actcccggca cattgctcga gcgtggccgc ctgctcgatg
      781 tgctgcgtca ggcgggtgca cctgcagcaa tgtgacattc tgcacagacc gccggcgatc
      841 aacatcctag cctccccgtc atggaaccat cgtgttggcg tggttccctg tcttcggaga
      901 ggtcgaacat catgaagtcc cgtgctttgt acaatcgatt tctaagtctg tccgccacgg
      961 cgctggcgct ttggacattc tggttgcccg cagaggccga agccagatgc ctcgatgtcg
     1021 ccctggtgct ggccatcgac gggtccagca gcgtggacag cgccgagtac cagttgcaga
     1081 agcaaggcat cgccgaagcg ctgcgcgatc cccaggtcat tgccgccatg cggcaggccg
     1141 gcgatgtcgc cgtatcggtg atcttctggg gcgatgcggg tcagccacat cagaaaatcg
     1201 actggttccg cattgccgac agcaaggacg gagatcgact ggcccaacgc atcgagcgca
     1261 tgcatcgcaa cgtgaagggc agcaccggac tgggaaccgg ccttgtggca gcgctcgaca
     1321 tgatcgcgga cgcggacttc tgcgccaacc gcgccatcat caacgtctcg ggcgacggtt
     1381 cggagaccgt ctaccggcgg cagacccgcc atctgcccaa gccggaagac gcacgcgccc
     1441 gggccgagga ggccggcgtc acgatcaatg ccctcgccat cagcaaccag gagggcgggc
     1501 tgaaggacta cttcgccagc caggtcgtaa ctggaccggg cgccttcgtg atggaggtca
     1561 agaactacga gagtttcgcc gaggccatgc ggcgcaagct gatccgcgag atcaccccca
     1621 gggcagtgag cggcctccgc ttgccggtcg cctggtccga cccgccgcgc gcgcccgatc
     1681 ggccctgaac gcctgacgac atggcgagcc cagctagcca tgctcccggt atcctgctat
     1741 ctcgcccggc accacgtcgc atgaggatac cgcatcttgg ccaccccaat tatgaacgtc
     1801 atgatcggcg ccgtccgcaa ggccgcccgc agcctgcagc gcgactatgg cgagctttcc
     1861 aatctgcagg tgtcgcagaa ggccccgggc gactacgtca ccgcggccga caagaaatgc
     1921 gacaagatcc tgcgcgagga gttgcagaag gcccggccgg actacacctt cctgaccgag
     1981 gaatcgggaa ccatcaaagg aagcgatccc gaccatcg
//
LOCUS       DBNGVR010000305         2851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3452497, whole genome shotgun sequence.
ACCESSION   DBNGVR010000305 DBNGVR010000000
VERSION     DBNGVR010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3452497"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC16A0_06500"
     CDS             complement(<1..794)
                     /locus_tag="AC16A0_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HIR3105018.1"
                     /translation="MTTAQSFITLTYLIETAGNPEALAAQIASDQSTGTFVAVPGETE
                     ELKNRVAARVVALRPLPPVAVPSFPSDAAGPFHRAEADIAFPFEAIGTDLAALMTIAI
                     GGVYSIRGFTGIRIIDMKLPEAFGKAWPGPQFGVAGTRRLTGVHDRPIIGTIIKPALG
                     LRPHETAALVRDVIDAGVDFVKDDEKLMSPAYSPLEARVRAIMPMILEREQKTGRKVM
                     YAWGISATDPDEMMRNHDVVVKHGGNCAVININSIGFGGMSFLRKRS"
     gene            848..1567
                     /locus_tag="AC16A0_06505"
     CDS             848..1567
                     /locus_tag="AC16A0_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HIR3105019.1"
                     /translation="MDVSERISKRKLSEEVFERLKHMIVEGAMTPGSPMPSERELMTR
                     FGVGRAAIREAMQQLANIGLIEISHGEKARVLELTPQSLMRQVDESARIMLSLSHDSL
                     EHLKSARIFFERGMARAAALKSTDEDLKQLRETLEEQRRSLGDAERFIAADMRFHTQI
                     AAISGNPIYTAVSEAMLGWLKTYHGEMLIWTGKEKYTLAEHEEIISHLAANDPDAAEA
                     AMVKHLERSRALYVSNRQATR"
     gene            complement(1564..2598)
                     /locus_tag="AC16A0_06510"
     CDS             complement(1564..2598)
                     /locus_tag="AC16A0_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3105020.1"
                     /translation="MAQLRGALIGCGFFAINHLHGWHDAAGAEIVAICDRNPERLAIA
                     GDSFGIMRRYAEADEMLRAERPDFVDIATTAPSHRPLVELAAGLGIPVIVQKPFATAL
                     ADAKAMVQACKAAGVPLMVHENFRWQSPILKVREILQSGEIGEVFWGRVSFRSAYDVF
                     SGQPYLAEGERFIVEDLGIHALDVARFLFGDATNVSARIKRVNPAIRGEDVATALLDH
                     GNGVASVVDCSYATRLERELFPQTLLEIDGTKGSLRLGPGYELTVTAGGQTRKERIAP
                     RLLPWATRPWHNIQESVALIQQHWVDCLAKGLEPATSGRDNLQTLALVEAVYRSAATG
                     RTVMLSELLD"
     gene            complement(2601..>2851)
                     /locus_tag="AC16A0_06515"
     CDS             complement(2601..>2851)
                     /locus_tag="AC16A0_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018245456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105021.1"
                     /translation="LNAIAAAVLGGTSMSGGRGTIGGTIIGAFVIGVLSDGLVMMGVS
                     SFWQMVIKGLVIIFAVVIDQAQRRLQQRVTLMRMARLG"
BASE COUNT          536 a          919 c          933 g          463 t
ORIGIN      
        1 gagcgtttgc gcaggaagct catgccgccg aagccgatgg agttgatgtt gatgacggcg
       61 cagttgccgc catgcttcac gaccacgtca tggttgcgca tcatctcgtc cgggtcggtc
      121 gccgagatgc cccaggcata catgaccttg cggccggtct tctgctcgcg ctcgaggatc
      181 atcggcatga tcgccctgac gcgcgcctcc agcggcgaat aggccggcga catcagcttc
      241 tcgtcgtcct tcacgaaatc cacgccggcg tcgatgacat ccctgaccag cgccgccgtc
      301 tcatgcgggc gcaggcccag ggcgggcttg atgatggtgc cgatgatcgg cctgtcgtga
      361 accccggtca gccggcgcgt tccggccacg ccgaactgcg gaccgggcca ggcctttccg
      421 aaggcctcgg gcaacttcat gtcgatgatg cggatgccgg tgaaacccct gatcgaatag
      481 acgccgccga tggcgatggt catcagggcc gcgaggtcgg taccgatggc ctcgaaggga
      541 aaggcgatgt cggcttccgc ccgatggaag ggccccgccg cgtccgacgg aaaggacgga
      601 acggcaaccg gcggcagggg cctcagcgcc accacccgtg cggcaacccg gttcttcaac
      661 tcctcggttt cgccgggaac ggccacgaag gttccggtcg actgatcgct ggcgatctgc
      721 gcggccaggg cctcgggatt gccggcggtc tcgatcagat aggtcagggt aatgaaggac
      781 tgggctgttg tcatgaccgg aagctgctaa ggttgatctg ctgatctgga gcgcgagcaa
      841 gcataccatg gatgtgtcgg aacgcatttc caagcgcaag ctctcggagg aggtcttcga
      901 gcggctgaag cacatgatcg tcgaaggggc catgacgccg ggaagcccca tgccctccga
      961 acgcgagctg atgacccgct tcggcgtggg gcgcgccgcc atccgcgagg ccatgcagca
     1021 gctggcaaac atcgggctga tcgagatcag ccatggcgag aaggcccgcg tgctggagct
     1081 cacgccgcaa tccctgatgc ggcaggtgga cgagtcggca cgcatcatgc tgtccctgtc
     1141 gcatgactcg ctggaacatc tgaagagcgc gcgcatcttc ttcgagcgcg gcatggcgcg
     1201 ggcggcggcc ctcaagtcga ccgacgagga tctgaagcag ctgcgcgaga cgctggagga
     1261 acagcgcagg tcgctgggcg atgccgaacg gttcatcgcc gccgacatgc gcttccacac
     1321 ccagatcgca gcgatttccg gcaatccgat ctacacggcc gtcagcgaag ccatgctggg
     1381 ctggctcaag acctaccacg gcgagatgct gatctggacc ggcaaggaaa aatacacgct
     1441 ggccgaacat gaggagatca tctcgcacct ggccgccaat gatcccgacg cggcggaggc
     1501 cgcgatggtg aagcatctcg agcggtcgcg cgcgctttac gtatccaacc ggcaagcgac
     1561 acgctagtcc agcagctcgg agagcatgac cgtccgcccg gtcgcggccg atcgatagac
     1621 cgcctcgacc agggccaggg tctggagatt gtcgcggccg gaggttgcgg gctccagccc
     1681 tttggcgagg cagtcgaccc aatgctgctg gatcagggct acgctctcct ggatattgtg
     1741 ccagggccgc gtggcccagg gcagcagacg gggcgcgatc cgttccttgc gcgtctggcc
     1801 gccggccgtg acggtgagtt catagcccgg gccgaggcgc aggcttccct tcgtgccgtc
     1861 gatctccagc agggtctgcg gaaacagctc gcgttcgagc cgggtcgcat aggagcagtc
     1921 gaccacggag gccacgccat tgccatggtc gagcagcgcg gtcgcgacat cctcgccccg
     1981 gatcgccgga ttgacccgct tgatgcgggc actgacattc gttgcatcgc cgaagagaaa
     2041 gcgcgcgacg tccagggcat ggatgccaag gtcctcgacg atgaagcgct cgccctccgc
     2101 cagataaggc tgccccgaga agacatcata ggccgagcgg aaggacacgc gtccccagaa
     2161 gacctcgccg atctctccgc tctgcaggat ctcccggacc ttcaggatcg gcgattgcca
     2221 gcggaaattc tcatgcacca tgagcggcac gcctgcggcc ttgcaggcct gcaccatggc
     2281 cttggcatcg gccagcgcgg tggcgaaggg cttctgaacg atgacgggga tgccgagacc
     2341 ggccgccagt tccaccaagg gccggtggct tggcgccgtg gtggcgatgt ccacgaagtc
     2401 aggccgttcc gcacgaagca tctcatccgc ttcggcatag cggcgcatga tgccgaagct
     2461 gtcgccggcg atggccaagc gctctggatt cctatcgcag atggccacga tctccgcccc
     2521 ggcggcatca tgccagccat gcaggtgatt gatggcgaag aaaccgcacc cgatcagcgc
     2581 accgcgaagc tgcgccatgg tcaacccagc cgcgccatgc gcatcagcgt cacgcgctgc
     2641 tgcaatcgcc gttgcgcctg gtcgatgacc acggcgaaga tgatgaccag gcccttgatc
     2701 accatctgcc agaaggaact gacgcccatc attacaagcc cgtccgacag gacgccgatg
     2761 acaaaggcgc cgatgatggt gccgccgatg gtgccgcgtc cgccggacat ggatgtacca
     2821 ccgagaaccg ccgcggcaat ggcattgagc t
//
LOCUS       DBNGVR010000306         2670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1684622, whole genome shotgun sequence.
ACCESSION   DBNGVR010000306 DBNGVR010000000
VERSION     DBNGVR010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1684622"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="AC16A0_06520"
     CDS             <1..734
                     /locus_tag="AC16A0_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3105022.1"
                     /translation="ALETLKQRINHVEPPADRPAAKSDQIYEYLRRAIVRLELQPGSA
                     IQEKDIATRFEVSRTPVREAVQRLAEEDLVHIYPHSGTWVSRISFRVAEEGFVLRRAL
                     EVESVRKAASRITEADAAQLNATIARMRVILQEDRLADYLEEDDAFHASIARISGYPR
                     IWKFITLAKVHLDRMRQLSAPVPGHLAEVTEQHAAIAQALQRGNAMQAELAMRIHLDS
                     SFAVMAGMYDGKAAIFADDAEDPAQ"
     gene            753..2297
                     /locus_tag="AC16A0_06525"
     CDS             753..2297
                     /locus_tag="AC16A0_06525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3105023.1"
                     /translation="MSGLRESYDAIIIGAGVGGGAMANQLAAAGARVLVIERGTRLPR
                     EDSNWSVKAVFHEKRYATKETWRDRDGQEFNPSTYYYVGGNSKFFGTATLRLRVEDFE
                     ELQHEAGVAPAWPIRYADLAPYYDVAEQVFGTHGTAGMDPCEPPRSGPMPYPAIGHEP
                     EIQAFDTRLRSMGLKPFPLPVAIDLHPGGRCIRCRTCDGFACKLGAKNDAEVRMVDPA
                     LATGNVDLILETYVHRLVTDDTGRKVVGVEVEQKGQKKTIAGDLFVSSAGAINSAALL
                     LRSANDKHPKGLGNNSSDQLGRNYMAHNNTAMMAVNPFKKNNVVFQKSLCINDFYLAN
                     SVRPYPLGNVQGLGKLQGGMLTANMKGIPEFLMDWFATRSVDWWIMSEDLPDPNNRVS
                     LDGDGKIRLSYTPNNLKSHAELVKHWTKLMRSMGYPIIITQKMDIKVAMHQCGTARFG
                     NDPSTSVLDPFCKAWDVDNLYVVDASFMPSSTSVNPSLTIAAQAVRTTEHLLRDVFKI
                     RKPAAA"
     gene            complement(2303..>2670)
                     /locus_tag="AC16A0_06530"
     CDS             complement(2303..>2670)
                     /locus_tag="AC16A0_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3105024.1"
                     /translation="FRRLTLNAGDVLLRQGAESREMFVLISGALRAEVARSTGEPLVV
                     ARFMPGAPIGEIAFYGNVPRTATVLAEEPSVLVKIDAASLVRSGDDHDPATVVHHYVA
                     VNLARRLMNMTRLMRDADL"
BASE COUNT          528 a          882 c          820 g          440 t
ORIGIN      
        1 tggccctcga aacgctcaaa caacgaataa atcatgttga gccgcctgcg gatcggccag
       61 cagcaaagtc tgaccagata tatgagtatc tgcgccgggc catcgtcagg ctggaactgc
      121 agcccggatc ggccatccag gaaaaggaca tcgccacccg cttcgaggtc tcgcgtacgc
      181 cggtgcgcga agccgtgcag cggcttgccg aggaggatct cgtccacatc tatccgcatt
      241 ccggcacctg ggtcagccgc atctccttcc gggtcgcgga ggaaggcttc gtgctgcggc
      301 gggcgctcga ggtggaaagc gtgcgcaagg ccgccagccg catcaccgaa gctgatgccg
      361 cgcaacttaa cgccacgatc gcccgtatgc gggtcatcct gcaggaggac cggcttgccg
      421 actatctgga ggaggacgac gccttccatg cgtccatcgc acgcatcagc ggctatcccc
      481 gcatctggaa attcatcacg ctggcgaagg tccatctcga tcgcatgcgg cagctgagcg
      541 cgccggttcc cggccacctt gccgaggtga cggaacagca tgccgccatc gcgcaggcgc
      601 tgcagcgcgg caatgccatg caggcggaac tcgccatgcg catccatctc gacagttcct
      661 ttgccgtgat ggccggcatg tatgacggca aggcggcgat cttcgccgac gacgcggaag
      721 atccggcgca atgacaagat gacgaggtcg acatgagcgg tttgcgggag tcctatgacg
      781 ccatcatcat cggtgcgggc gtgggcggcg gcgccatggc caaccagctc gccgcggccg
      841 gcgcgcgcgt gctggtcatc gagcgcggca cacggcttcc gcgcgaagac tcgaactgga
      901 gcgtcaaggc ggtcttccac gagaagcgct acgccacgaa ggaaacctgg cgcgaccgcg
      961 acggtcagga gttcaatccg tcgacctatt actatgtcgg cggcaattcg aagttcttcg
     1021 gcaccgcaac gctgcgcctg cgggtggaag acttcgagga gctgcagcac gaggccgggg
     1081 tggcacccgc ctggcccatc cgctatgccg atctggcgcc ctattacgat gtggccgagc
     1141 aagtcttcgg gacccatggc accgccggga tggacccctg cgaaccgccg cgctccggcc
     1201 ccatgcccta tcccgcgatc ggccacgagc ccgagatcca ggccttcgac acgaggctgc
     1261 gcagcatggg actgaagccc ttcccgcttc ccgttgccat cgatctgcat cccggcggac
     1321 gctgcatccg ctgccgcacc tgcgacggct ttgcctgcaa gctgggcgcc aagaacgacg
     1381 ccgaggtccg catggtcgat ccggcgctcg ccaccggcaa tgtcgatctc atcctcgaga
     1441 cctatgtgca tcgtctcgtc accgacgata ccgggcgcaa ggtggtcggc gtggaggtcg
     1501 agcagaaagg tcagaagaag accatcgccg gcgatctctt cgtctcaagc gcgggcgcca
     1561 tcaattcggc ggccctgctg ctgcgctcgg ccaacgacaa gcacccgaag ggactgggca
     1621 acaacagttc ggaccagctg ggccgcaact acatggccca caacaacacc gccatgatgg
     1681 cggtgaatcc cttcaagaag aacaacgtcg tcttccagaa gtcgctgtgc atcaacgatt
     1741 tctatctcgc caacagcgta aggccctatc cgctcggcaa tgtgcagggc ctcggcaagc
     1801 tgcagggcgg catgctgacg gcgaacatga agggcattcc cgaattcctg atggactggt
     1861 tcgccacgcg cagcgtcgac tggtggatca tgtcggaaga ccttccagac ccgaacaaca
     1921 gggtcagcct cgacggcgac ggcaagatcc ggctctccta cacgccgaac aatctgaagt
     1981 cccatgccga actggtgaag cactggacaa agctgatgcg ttcgatgggc tatccgatca
     2041 tcatcaccca gaagatggac atcaaggtgg cgatgcatca gtgcggaacg gcgcgcttcg
     2101 gcaatgatcc atccacctcc gtgctggacc ccttctgcaa ggcctgggat gtcgacaacc
     2161 tctacgtggt cgatgcgtcc ttcatgccgt catcgacctc cgtcaatccg tcgctcacca
     2221 ttgcggccca ggcggtgcgg accaccgaac acctgctgcg cgatgtcttc aagatccgca
     2281 agccggccgc ggcctgacgc gatcagagat cggcatcgcg catgaggcgc gtcatgttca
     2341 tcagccggcg ggcaaggttg acggcgacat aatggtgaac cacggtcgca ggatcgtggt
     2401 catcgcccga acggacgaga cttgcggcat cgatcttcac cagtaccgag ggttcttccg
     2461 ccagcacggt ggcggtgcgc ggtacgttgc catagaaggc gatctctccg atgggtgcgc
     2521 cgggcatgaa gcgcgccacc accaggggct cgccggtcga gcgcgccacc tcggcccgca
     2581 aggcgcccga gatcagcacg aacatctcgc gcgactcggc cccctggcgc agcagaacgt
     2641 cgcccgcatt gagcgtcagc cgccggaact
//
LOCUS       DBNGVR010000307         2946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3648883, whole genome shotgun sequence.
ACCESSION   DBNGVR010000307 DBNGVR010000000
VERSION     DBNGVR010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3648883"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..748
                     /locus_tag="AC16A0_06535"
     CDS             185..748
                     /locus_tag="AC16A0_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105025.1"
                     /translation="MRQISNLRLKSEFLGWQCRIRQISARDYGGQPMPAMHPRVSSRK
                     GEVLLPAMMILLVPDDTVPATAFLKFQVQKTSEHQRAREASVKYFGGDFYQLPELFSD
                     EMTAVFGPGSETAARLLKQKEVLLDFEQYAQSYRMFCKVRKLGPRDGALDFSLWHARV
                     FNPNVPNDSQVLGFKPDWKSAVGASSA"
     gene            745..1296
                     /locus_tag="AC16A0_06540"
     CDS             745..1296
                     /locus_tag="AC16A0_06540"
                     /inference="COORDINATES: protein motif:HMM:NF017747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD/Ves family protein"
                     /protein_id="HIR3105026.1"
                     /translation="MITVLPSSGFCQGRWLNGMGVSWDIAAEPENAGSADFAWRFAIA
                     RIDADVPFSRYSGVDRIFTLIEGEGLTLDLEGHAPLIIDRRFVPQAFSGDVATSCRLN
                     AGPCRALNLFLDRGSWGAEVNIVRGAGQLAGGETLLLFALDGDVALEDRSLHAGDAAL
                     ASGVLHYAAGPHHLYAARLFQKT"
     gene            complement(1355..1768)
                     /locus_tag="AC16A0_06545"
     CDS             complement(1355..1768)
                     /locus_tag="AC16A0_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105027.1"
                     /translation="MAGTSYKGSEIKDVDEFIKNKAGLSSSREKEIGQHIGYRYDVNL
                     IPDYKRMTPFLKGYMEYMGWDDLNWLEDVHMGYEEGEPAIFDRNVNGWVRIPKKIKLP
                     DNQQDRDMMARELLIKFQMSPKHPMVNLNKAYRKF"
     gene            complement(1781..>2946)
                     /locus_tag="AC16A0_06550"
     CDS             complement(1781..>2946)
                     /locus_tag="AC16A0_06550"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR3105028.1"
                     /translation="REKCETKTIIGPRAKRPMVLDIPIYVTGMSFGALSYEAKIALAR
                     GATMAGSATCSGEGGMIPDERRYSERWLYQCIQSRYGFNPHHLRLADACEFFIGQGCK
                     VGLGGHLMGQKVTDQVAEMRSLPAGIDQRSPARHPDWLGPDDLALKIQEIREATNHEI
                     PIQLKLGAARVYDDVRMAVKCDPDSIYIDAMEGGTGAGPHIATEDTGVPGIAAIREAR
                     RAIDELGKKGQISLVYAGGIRNGGDVAKALALGADAIAIGHSVMMALNCNKHIDGVTD
                     YPNEIGVEAGFCYHCHTGRCPVGVATQDPVLRQRLDPDKAAERVYNFLHTLTMEAQLL
                     ARACGKTNIHSLEPEDLAALTMEASAMCKVPLAGTNYVVGHEALGTGGNYHKY"
BASE COUNT          489 a          920 c          939 g          598 t
ORIGIN      
        1 cttcttggcg gcggactgga aggctctgag ggcagcaagg ccggaagggc ctgctccgat
       61 gacggcgaca cgggtcatgc gacactttcc tccgttgtgg cggttccgct tgcatacgga
      121 atccaattct gaccacatta ggccaattcc ggaccaatct gtaaagggtc aaaccgcctc
      181 aatcatgcga caaatcagca atcttcgact gaagtctgag ttcctgggct ggcagtgccg
      241 catccgccag atctccgcac gcgactatgg gggccagccg atgcccgcca tgcatccccg
      301 cgtcagcagt cgaaagggcg aggtgcttct gccggccatg atgatccttc tggtaccgga
      361 cgacacggtg ccggccaccg ccttcctcaa gttccaggtg cagaagacat ccgaacacca
      421 gcgcgcccgc gaggccagcg tgaagtactt cggcggcgac ttctaccagt tgccggaact
      481 tttctcggac gagatgacag ccgtcttcgg tcccggatcg gagacagcgg cacggcttct
      541 caagcagaag gaggtgctgc tcgacttcga acagtatgcc cagtcctatc gcatgttctg
      601 caaggtgcgg aaactggggc caagggacgg tgcgctcgac ttcagcctgt ggcatgcccg
      661 ggtcttcaat cccaatgttc cgaacgactc gcaggtcctg ggcttcaagc cggattggaa
      721 gagtgcagtg ggcgcatcct ccgcatgatc acggttctgc cgtcttccgg attttgccaa
      781 gggcggtggc tcaatggcat gggcgtgtcg tgggacattg ctgccgaacc cgagaatgcc
      841 gggtcagcgg acttcgcgtg gcgcttcgcc atcgcccgca tcgacgcgga cgttcccttc
      901 tcgcgctatt ccggcgtcga tcgcatcttc accctgatcg agggcgaagg actgacgctc
      961 gatctggaag gtcatgcgcc gctgatcatc gaccgcaggt tcgttcccca ggccttttcg
     1021 ggcgacgtgg cgacctcgtg ccggttgaat gccggccctt gccgcgcgct caatctcttt
     1081 ctggaccggg gatcttgggg cgcagaggtg aacatcgtgc ggggggcagg gcagctggca
     1141 ggcggcgaaa ccctgctgct cttcgcgctg gacggggatg tcgcgctgga ggaccgtagc
     1201 ttgcatgctg gcgatgccgc cttagcatcg ggtgttctgc actacgcggc cggcccacac
     1261 catctctacg cggcgcggct cttccaaaag acgtaaggcc cgggatttct cccgggccct
     1321 gcgatgaccg cgttgatgcg cgactgccgc ccgatcagaa cttgcgatag gctttgttca
     1381 gattgaccat cggatgcttc ggcgacatct ggaacttgat gagaagttcc cgggccatca
     1441 tgtcgcgatc ctgttggttg tcgggcagct tgatcttctt ggggatgcgc acccatccgt
     1501 tgacgttgcg gtcaaagatg gcgggctcgc cttcctcgta acccatgtgg acatcctcga
     1561 gccagttcag gtcgtcccag cccatgtact ccatgtagcc cttcaggaag ggcgtcatcc
     1621 gcttgtagtc ggggatcagg ttcacatcgt aacgatagcc gatgtgctgt ccgatctcct
     1681 tctcgcggct ggaggaaagg cccgccttgt tcttgatgaa ctcgtcgacg tccttgattt
     1741 cggagccctt gtagcttgtg cctgccatgt tcgtctccga tcagtacttg tgataattgc
     1801 cgcccgtgcc gagcgcctcg tggccgacga catagttggt gccagccagc ggaaccttgc
     1861 acatggccga ggcttccatg gtgagcgcgg cgagatcttc cggctccagc gaatggatgt
     1921 tggtcttgcc gcaggcgcgc gccagcaact gggcctccat cgtcagcgtg tgcaggaagt
     1981 tgtagacgcg ctccgccgcc ttgtcgggat cgagccgctg gcgcagcacc gggtcctggg
     2041 tggccacgcc caccgggcaa cggcccgtat ggcaatggta gcagaagccc gcctcgacgc
     2101 cgatctcgtt gggatagtcg gtgacgccgt cgatatgctt gttgcagttc agcgccatca
     2161 tcacggagtg gccgatggcg atggcatcgg cgcccagcgc cagggccttt gccacgtcgc
     2221 cgccgttgcg gatgccgccg gcatagacca gcgagatctg gcccttcttg ccgagctcgt
     2281 cgatcgcgcg gcgcgcctcg cggatcgccg cgatgcccgg cacgccggtg tcttcggtcg
     2341 cgatatgcgg gcctgcaccc gtgccgcctt ccatggcgtc gatgtagatc gaatccggat
     2401 cgcacttcac tgccatgcgg acgtcgtcgt agacgcgcgc cgcgccgagc ttcagctgga
     2461 tcggaatctc gtggttggtg gcctcgcgga tttcctggat cttgagcgcc aggtcgtcag
     2521 gccccagcca gtcgggatgg cgggccgggg agcgctggtc gatgccggcg ggcagtgagc
     2581 gcatctcggc cacctggtcc gtcaccttct ggcccatgag atggccgccg aggcccacct
     2641 tgcagccctg accgatgaag aactcgcagg catcggcaag gcgcaggtgg tgcgggttga
     2701 agccgtagcg cgactggatg cactggtaga gccagcgttc ggaatagcgc cgctcatccg
     2761 ggatcatgcc gccttcgccg gagcaggtgg cggaacccgc catcgtcgca ccgcgcgcca
     2821 acgcgatctt cgcttcatag gacagagcgc cgaacgacat gccggtgacg tagatcggaa
     2881 tgtcgagcac catgggccgc ttggcgcgcg gcccgatgat ggttttcgtc tcgcacttct
     2941 cgcgat
//
LOCUS       DBNGVR010000308         2089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4238610, whole genome shotgun sequence.
ACCESSION   DBNGVR010000308 DBNGVR010000000
VERSION     DBNGVR010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4238610"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /gene="malQ"
                     /locus_tag="AC16A0_06555"
     CDS             <1..1998
                     /gene="malQ"
                     /locus_tag="AC16A0_06555"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR3105029.1"
                     /translation="WSRSFSSAEGTDVPPQAVALYTEERVQRSGVSDSTIVQLATAAG
                     IERDWWEVDGTHHLVTPETLRHLLSALRIAHASPEEAEQSLRQLRMRQAPQVIAAGEM
                     AVIGAASDKRQVITIESEDGQRQTVTVVPGAIPHARLDMGYYKVWSGEDPSAARSLIV
                     TPGACHLPDDIAAGHRVYGLAAHLYALRHDGDGGIGDLETLRRFADVTSEIGGRYAGL
                     NPLHHMFPADRSRASPYQPSDRRYVDPIYINIPQLLEDLALPKAAALAETRRAAFADL
                     AALSHVDYHAVWAAKSALLEQAFKEFHSNEAFEAFIAAGGAALRRHGAFEAQQAGEPA
                     SPRRVAYRAFLQWIAETQLARAARRGNLYRDLALGSAFDGGEIAESPDDYAHGVSLGA
                     PPDPFSREGQVWNLPPLSPLALAERRFQPLRDVMLANMRHAAALRIDHVLGFMRQFWV
                     PRGAEGKAGAYVNFPIDGMIAVTAIESRRQNCMVIGEDLGTIPDGLRERLNAARILSY
                     RVLWFERMGSGFRPPTDYPELALACLASHDLPTFRGWRQGLDIAIDRGLGRIPPDAVS
                     AREEARREEIAQLDSLSRAASNGLQDASVAAHGMIASAPSRVMLIQTDDLADASEPLN
                     VPGTDTERPNWRRRLLPTVDRLAATPLAQAIIARVKAERGT"
BASE COUNT          333 a          698 c          693 g          365 t
ORIGIN      
        1 tggtcgcgca gcttcagctc agcggaaggc acggatgtac caccgcaagc cgtggcgctc
       61 tacaccgaag agcgcgtgca gcggagcggg gtctctgaca gtacgattgt gcagctggcc
      121 accgccgccg ggatcgaacg cgactggtgg gaggtggatg gcacgcatca tcttgtgacg
      181 ccggaaacct tgcgccatct gctctcggcc ttgcgcattg cgcatgcatc gcccgaagag
      241 gccgagcagt ccttgcggca gctccggatg cggcaggctc cgcaggtgat tgccgcgggc
      301 gagatggccg tgatcggtgc tgccagcgac aagcgtcagg tcatcacgat cgagtcggaa
      361 gatggccaac gccagactgt cacggtggtc cccggcgcga ttccgcacgc gcggctggac
      421 atgggctact acaaggtgtg gagcggtgaa gatccgtcgg cggcacgcag cctgatcgtg
      481 acccctggcg cctgccatct ccccgatgat atcgccgccg gacaccgggt ctacggtctc
      541 gccgcgcacc tctatgccct gcgccatgac ggcgatggcg gcatcggcga tctcgagacc
      601 ttgcggcgct tcgccgatgt gacatcggag atcggcggcc gctatgcggg gctcaacccg
      661 ctccatcaca tgttcccggc cgaccggtcg agggccagcc cgtaccagcc atcggaccgg
      721 cgctacgtgg atccgatcta catcaacatt ccgcagcttc tcgaagatct ggctctgccg
      781 aaagcggctg cgctggcaga gacgcgccgc gcggcctttg ccgatctcgc ggcactgtcg
      841 catgtcgact atcatgcggt ctgggctgcc aagagtgccc tgctggagca ggccttcaag
      901 gagttccaca gcaacgaggc cttcgaggcc ttcatcgccg ccggcggcgc tgcgctgcgc
      961 cgccatggcg ccttcgaagc gcaacaggca ggcgaacccg cctcgccccg gcgtgtggcc
     1021 tatcgcgcct tcctgcaatg gatcgccgag acgcagcttg cgcgcgccgc ccgcagggga
     1081 aatctctatc gtgacctggc gctcggctcg gccttcgatg gcggcgagat cgccgagtcg
     1141 ccggatgact acgcacatgg cgtgtcgctc ggcgcaccgc cggatccctt ctcgcgcgag
     1201 ggccaggtct ggaatcttcc gccgctgtcg ccgcttgcgc tggcagaaag gcgtttccag
     1261 cctttgcgcg acgtgatgct ggccaacatg cggcacgccg ctgcgctgcg catcgaccat
     1321 gtcctgggct tcatgcggca gttctgggtg ccgcggggtg cggagggcaa ggccggcgcc
     1381 tatgtgaact ttccgatcga cggcatgata gcggtgacgg ccatcgaaag ccggcggcag
     1441 aactgcatgg tgatcggcga ggaccttggc accatccccg atggactgcg cgaacggctc
     1501 aatgccgcgc ggatcctgtc ctatcgcgtt ctctggttcg agcgcatggg atcagggttc
     1561 aggccgccca ccgattatcc ggagctggcg ctggcttgcc tcgcctcgca tgaccttccg
     1621 accttccgcg gctggcgcca gggcctcgac attgccattg atcgcgggct cggccgcatt
     1681 ccgccagacg ccgtctcggc acgcgaggag gcgcggcggg aggagatcgc ccagctcgat
     1741 tcgctttcgc gggctgcgtc gaacggcttg caggatgcct ccgtcgcggc acatggcatg
     1801 atcgcgtccg cgccctcgcg cgtgatgctg atccagaccg acgatctcgc cgacgcctcg
     1861 gaaccgctca atgttccggg caccgatacc gaacggccca actggcggcg caggctgctg
     1921 cccactgttg atcgattggc agcaacaccg ctcgcgcagg ccatcatcgc gcgggtgaag
     1981 gcggaacgtg gtacatgagc gatccggtga agatcgagag cgtcgagacg ctgtcgcatg
     2041 cctgggcgag gctcgaccgc tatgtcattt cctatcgccg tcgcgatgg
//
LOCUS       DBNGVR010000309         4612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2864058, whole genome shotgun sequence.
ACCESSION   DBNGVR010000309 DBNGVR010000000
VERSION     DBNGVR010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2864058"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="AC16A0_06560"
     CDS             complement(<1..799)
                     /locus_tag="AC16A0_06560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3105030.1"
                     /translation="MSKLPARNSVTGFLWRLDAIVSTAVFETWDWIKRASSAYSAFVY
                     RFRLRGIRRVFVDLLDDAVTFGVVFCFGLLAFALPPFSGTGDVWNRGREYAVTFTDAN
                     GEIIGRRGIRQDDAIPLGEIPPNLIKAALATEDARFFQHFGVDVVGTLRAIVENARAN
                     DVVQGGSSITQQVAKNLFLSPERTIRRKVHEAFLSLWIEARLSKEEILKLYLDRSYLG
                     GGNYGVEAASQFYFGKSVRDINLSESAMLAGLFKAPSKYAPHQNPEAA"
     gene            953..1456
                     /locus_tag="AC16A0_06565"
     CDS             953..1456
                     /locus_tag="AC16A0_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HIR3105031.1"
                     /translation="MTRRGASASDAAMTVEPPFWKAKSLAEMSRAEWESLCDGCGRCC
                     LNKLEDEDTGQFLYTRAACKLLDLNSCRCSDYPNRARRVSDCVSLTPENVGTLGWLPA
                     TCAYRLLDEGKPLAWWHPLVSGDPATVDEAGISVKGQAYSEEGIAVDDLVDHLWRLPK
                     ARRRTRR"
     gene            complement(1481..1708)
                     /locus_tag="AC16A0_06570"
     CDS             complement(1481..1708)
                     /locus_tag="AC16A0_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HIR3105032.1"
                     /translation="MELKDMVRMANQIASFYHGYPAEEAQKEIADHINRFWEPRMREE
                     FFAHLAKGGAGLDPVVIAAAAGIHPPHQAAA"
     gene            complement(1713..2537)
                     /gene="fdhD"
                     /locus_tag="AC16A0_06575"
     CDS             complement(1713..2537)
                     /gene="fdhD"
                     /locus_tag="AC16A0_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR3105033.1"
                     /translation="MSRRSNNIVTLKARPGAATSRPARGVLHTLGGEVPTVWQVPEEV
                     PVALLFNSQNYAVMMATPADLEDFAVGFALAEGIVARAFDIGGVLVLPTAEGLAVDLA
                     VAEAHLNRGRMASRSLEGRTGCGLCGIEDIRDAIRMPGHRVPDVPLSAQAVARAYAEL
                     PRHQPMNEVNRTVHAAAWCSVDGEILLAREDVGRHNALDKLIGALARTDEGMAQGFVL
                     MSSRCSFELVQKCAIAGIGALATVSAPTALALTLARQAHLKLAALSQRGVMIFEED"
     gene            complement(2521..>4612)
                     /gene="fdhF"
                     /locus_tag="AC16A0_06580"
     CDS             complement(2521..>4612)
                     /gene="fdhF"
                     /locus_tag="AC16A0_06580"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR3105034.1"
                     /translation="RAEMRGEQLVRMVPWKDGKANRGHSCVKGRFAWGYATHKDRVTK
                     PMIRERITDPWREVPWDEAIGYAAARFAEIQKKYGRGSIGGITSSRCTNEEVFVVQKM
                     IRAAFRNNNVDTCARVCHSPTGYGLSKTFGTSAGKQDFKSVAASDVIVVIGANPTDGH
                     PVFASRMKKRLREGARLIVIDPRRTDIVRSPHIEAQHHLQLLPGTNVAIVNAMAHVIV
                     SEGLENRAYIAERCDLAEFTRWQAFIKDPVNSPEALESVTGVPASEVRAAARLYATGG
                     NGAIYYGLGVTEHSQGSTMVMAMANLAMLTGNVGRNGVGVNPLRGQNNVQGSCDMGSF
                     PHEFSGYRHVSDDTVRHMFEEIWRTTLDAEPGLRIPNMFDAAIGGSFKGIFVQGEDIA
                     QSDPNTKHVTHALESMECVVVQDLFLNETAAFAHVFFPGTSFLEKDGTFTNAERRINR
                     VRQVKAPMQGKHEWQVVCELATAMGYPMAYASASEIMDEIARVTPTFAGVSFDKIDKL
                     GSVQWPCNDKAPEGTPLMHVGGFVRGKGRFMVTEFVPTPEKTNRSFPLILTTGRILSQ
                     YNVGAQTRRTANVAWHKEDVLEIHPHDAEVRGIRDGAMVSVASRIGATTLRAVISERM
                     PQGVVYTTFHHPGSGANVITTEYSDWATNCPEYKVTAVQVTVSNQPSDWQKEWEEREE
                     ENKKIAEKPLVAAE"
BASE COUNT          784 a         1412 c         1594 g          822 t
ORIGIN      
        1 gggcagcttc cgggttctgg tgcggcgcat atttcgaggg cgccttgaac aggccggcca
       61 gcatggccga ttcggagagg ttgatgtcgc gcaccgactt gccgaaatag aactgcgagg
      121 cggcctcgac gccatagttg ccgccgccga gataggagcg gtcgagatag agcttgagga
      181 tttcctcttt gctcagccgc gcttcgatcc acagggacag gaaggcctca tgcaccttgc
      241 ggcggatggt gcgctctggc gacaggaaga ggttcttcgc cacctgctgg gtaatggatg
      301 aaccgccctg cacaacgtca ttggcccggg cattctcaac gatggcgcgc agcgtgccga
      361 cgacgtcgac gccgaaatgc tggaagaagc gcgcatcttc ggtggcgagt gccgccttga
      421 tgagattggg cggaatctcg cccagcggaa tggcgtcgtc ctggcggatg ccgcggcggc
      481 cgatgatctc gccattggca tcggtgaagg tcaccgcata ttcgcggccg cggttccaca
      541 cgtcgccggt acccgagaag ggcggaaggg cgaaagccag aagtccgaag cagaaaacta
      601 cgccgaaggt gacggcgtcg tcgagaagat cgacgaagac gcggcgaatg ccgcgcagcc
      661 ggaagcgata gacgaaggcc gaataggccg aactcgccct cttgatccag tcccaggttt
      721 cgaacacggc ggtgctgacg atggcgtcga gccgccagag aaagcctgtg acggaattgc
      781 gggccggcag tttggacacg tgtctcttga ccagtatgca gcgtgagggt ggctatctag
      841 ctggcgaata tgaccgaata gcgccgtccc tgacgacccg gactcaaacg cctgccttga
      901 gcggcgatta tagcccaagc cggtgatgcc gccagcccct ccgggctttc gattgacaag
      961 acggggggct tcggcttcgg atgccgccat gacggtcgaa ccccccttct ggaaggcaaa
     1021 atccctggcc gagatgtcgc gcgccgagtg ggagagcctg tgcgacggct gtggccgttg
     1081 ttgcctgaac aagctggagg acgaggatac gggccaattc ctctataccc gggcggcctg
     1141 caagctcctc gacctgaata gctgccgctg cagcgactat ccgaatcgcg cccgcagggt
     1201 gtcggactgc gtctcgctca cacccgagaa tgtggggacc ctgggctggc ttccggcaac
     1261 ctgtgcctac cgcctgctcg acgagggcaa gccgctggcc tggtggcatc cgctggtctc
     1321 gggcgatccc gccaccgtcg acgaagcggg catcagcgtg aagggccaag cttattcgga
     1381 agaggggatc gcggtcgacg accttgtcga ccacctctgg cgcttgccca aggccaggcg
     1441 ccgcacgaga aggtgaggcg gaggcgccgc ctcgtccgga tcaggccgcc gcctggtgcg
     1501 gcggatgaat gccggcggcc gcggcgatca ccacgggatc aagacctgcg ccgcccttgg
     1561 ccagatgggc gaagaactct tcgcgcatgc ggggctccca gaagcggttg atgtggtcgg
     1621 cgatttcctt ctgcgcctcc tcggccgggt agccgtgata gaacgaggcg atctggttgg
     1681 ccatgcggac catgtctttc agttccatcg tgtcagtcct cttcaaaaat cattacgccg
     1741 cgctgtgaca gggcggcaag tttcagatgg gcctggcgtg cgagcgtcag cgccagggcg
     1801 gtgggagcgg aaaccgtggc cagggcgccg atgccggcga tggcgcactt ctgtaccagt
     1861 tcgaaactgc agcggctgga catgagaacg aagccctgcg ccatgccctc gtccgtgcgg
     1921 gccagggcgc cgatcagctt gtccagcgcg ttgtggcggc cgacatcctc gcgtgccagg
     1981 aggatttctc cgtccacgga acaccaggcg gcggcatgca ccgtgcgatt cacttcgttc
     2041 atcggttggt gccgcggcag ttcggcatag gcgcgagcca ccgcctgggc cgagagcggc
     2101 acatcgggca cgcgatggcc cggcatgcgg atcgcgtcgc gaatgtcctc gatgccgcag
     2161 agtccgcagc cggtgcggcc ctcgaggctg cggctggcca tgcggccgcg gttgagatgg
     2221 gcctcggcaa cggcgagatc gacggcgagg ccctcggcgg tcggcaacac cagtacgccg
     2281 ccgatgtcaa aagccctggc cacgatgcct tcggccagcg cgaagcccac cgcgaagtcc
     2341 tcgagatcgg caggcgttgc catcatgacc gcgtagttct gcgagttgaa gagaagggcg
     2401 accggaactt cctccggcac ttgccagacc gtgggcactt cgccgcccag cgtgtgcagc
     2461 acgccgcgcg ccggccgcga tgtggccgcg cccggcctcg ccttcagggt aacgatgttg
     2521 ttactccgcc gcgacaagtg gcttctccgc gatcttcttg ttctcttcct cgcgctcttc
     2581 ccattccttc tgccagtcgg aaggctggtt cgagaccgtc acctgcacgg cagtcacctt
     2641 gtattcgggg cagttggtgg cccagtcgga atactcggtg gtgatcacgt tggcgccgga
     2701 ccccgggtga tggaacgtcg tgtagaccac gccctgcggc atgcgctcgg aaatcaccgc
     2761 gcgcagcgtg gtggcgccga tgcggctggc caccgagacc atggcgccgt ccctgatgcc
     2821 gcggacttcg gcatcatgcg gatggatctc caggacatct tccttgtgcc aggccacatt
     2881 ggccgtgcgc cgggtctggg cgccaacgtt gtattgcgac aggatgcggc cggtggtgag
     2941 gatgagcggg aaggagcggt tggtcttttc cggcgtgggt acgaattcgg tgaccatgaa
     3001 gcggcccttg ccgcgcacga agccgccgac atgcatgagg ggcgtcccct cgggcgcctt
     3061 gtcgttgcag ggccactgca ccgaacccag cttgtcgatc ttgtcgaagg agacgccggc
     3121 gaaggtgggc gtcacccgcg cgatctcgtc catgatctcg gaggccgagg cataggccat
     3181 gggatagccc atggcggtgg cgagttcgca gaccacctgc cactcgtgct tgccctgcat
     3241 cggcgccttg acctggcgca cgcggttgat gcggcgttcg gcattggtga aggtgccgtc
     3301 cttctcgagg aaggaggtgc cggggaagaa gacatgggcg aaggcggcgg tctcgttgag
     3361 gaagagatcc tgcaccacca cgcactccat ggattccagc gcatgggtca cgtgcttggt
     3421 gttgggatcc gactgggcga tgtcctcgcc ctgcacgaag atgcctttga agctgccgcc
     3481 gatggccgcg tcgaacatgt tggggatgcg caggcccggc tcggcatcga gcgtcgtcct
     3541 ccagatttcc tcgaacatgt ggcgcaccgt gtcgtcgctg acatggcggt agccggagaa
     3601 ctcgtgcggg aaagagccca tgtcgcagga gccctgaaca ttgttctgtc cacggagcgg
     3661 attgacgccc acgccgttgc gcccgacatt gccggtgagc atggcgagat tggccatggc
     3721 catgaccatg gtggagccct ggctgtgctc ggtgacgccg aggccatagt agatggcgcc
     3781 attgccgccg gtggcataga gccgcgcggc ggcgcggact tccgaggccg gaacgccggt
     3841 gacgctttcg agggcctccg gcgaattgac cgggtccttg atgaaggcct gccagcgggt
     3901 gaattccgcg agatcgcagc gctccgcgat ataggcccgg ttctcgaggc cttcggagac
     3961 gatcacatgg gccatggcat tgacgatcgc gacgttggtg ccgggcagga gctgcaggtg
     4021 gtgctgcgcc tcgatgtggg gcgagcgcac gatgtcggtg cggcgcggat cgatgacgat
     4081 gagcctggcg ccctcgcgca ggcgcttctt catgcgcgag gcgaagaccg gatggccatc
     4141 ggtgggattg gcgccaatca ccacgatgac gtccgaggca gcgacggact tgaagtcctg
     4201 cttgccggcc gaggtgccga aggtcttgga caggccatag ccggtgggcg agtggcagac
     4261 gcgggcgcag gtatcgacgt tgttgttgcg gaaggcggcg cggatcatct tttgcaccac
     4321 gaagacttcc tcgttggtgc agcgcgagga ggtgatgccg ccgatggagc cgcggccgta
     4381 cttcttctgg atctccgcga agcgcgccgc cgcatagccg atggcctcgt cccagggcac
     4441 ctcgcgccat gggtcggtga tccgttcacg gatcatcggc ttggtgacgc ggtccttgtg
     4501 ggtggcataa ccccaggcga agcggccctt gacgcaggaa tggccgcggt tggccttgcc
     4561 gtccttccac ggaaccatcc ggaccagctg ctccccgcgc atctcggcgc gg
//
LOCUS       DBNGVR010000310         1683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_312133, whole genome shotgun sequence.
ACCESSION   DBNGVR010000310 DBNGVR010000000
VERSION     DBNGVR010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_312133"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1380)
                     /gene="purB"
                     /locus_tag="AC16A0_06585"
     CDS             complement(49..1380)
                     /gene="purB"
                     /locus_tag="AC16A0_06585"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3105035.1"
                     /translation="MIPRYTRPEMAAIWEPQTRFRIWFEIEAHATDCLAELGVVPKAA
                     AAKIWEKGKNAVFDVARIDEIEAVTKHDVIAFLTHLSEIVGPEARFVHQGMTSSDVLD
                     TCFNVQLTRAADLLIADVDGLLAALKRRALEHRDTVCVGRSHGIHAEPTTFGVKLAQA
                     YAEFARGRERLVMARREVATCAISGAVGTFANIDPRVEEYVAAKMGLAPEPVSTQVIP
                     RDRHAMYFAALAVVASSIERLATEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVL
                     TENLTGLARLVRSAVIPALENVALWHERDISHSSVERGIGPDATVHLDFALKRMTSVI
                     DKLMVYPENMRRNLDRLGGLIHSQRVLLALTQKGMSREESYAAVQRNAMPVWRGEGNF
                     LDLLKADKEVSTLLGAAELEGLFDLGYHTKHVDTIFRRVFGADSQTAIKPA"
     gene            complement(1446..>1683)
                     /locus_tag="AC16A0_06590"
     CDS             complement(1446..>1683)
                     /locus_tag="AC16A0_06590"
                     /inference="COORDINATES: protein motif:HMM:NF021529.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HIR3105036.1"
                     /translation="ALTLKVLTTGKSVTIARDESIPKSRLCPLRYDIEAVYTPSGFGN
                     PGLQVALIGVYSRGFEGENRRIIAVPFELPSWEN"
BASE COUNT          286 a          518 c          582 g          297 t
ORIGIN      
        1 gaatggggtg attccccgcg tgaccacgcg caggcgatgg caatcgattt aggcgggctt
       61 gatcgcggtc tggctgtctg ccccgaagac acggcggaag atcgtgtcca catgcttggt
      121 gtgatagccc agatcgaaga ggccttcgag ttcggcggcg ccgagcaggg tggagacctc
      181 cttgtcggcc ttgagaaggt cgaggaagtt gccctcgccg cgccagaccg gcatggcatt
      241 gcgctgcacg gcggcatagg actcctcgcg gctcatgccc ttctgggtca gggcaagcag
      301 cacgcgctgc gaatggatca ggccgcccag cctgtcgagg ttgcgccgca tgttctcggg
      361 atagaccatc agcttgtcga tgacggatgt catgcgcttc agggcgaagt cgagatgaac
      421 ggtggcatcc ggtccgatgc cccgctcgac ggaggaatgc gaaatgtcgc gctcgtgcca
      481 gagggccaca ttctcgagag cggggatgac cgcggagcgc accagccggg cgaggccggt
      541 gaggttctcc gtcagcaccg gattgcgctt gtgcggcatg gcggaagatc ccttctggcc
      601 gggcgagaaa tactcctcgg cctccagaac ctccgtacgc tgaagatgcc ggatctcggt
      661 ggcgagcctt tcgatggaac tggccaccac ggcgagtgcg gcgaaataca tggcatggcg
      721 atcgcgcggg atcacctgcg tcgaaaccgg ttccggcgcc agccccatct tggccgcgac
      781 atactcctcc acccgcggat cgatgttggc gaaggttccg accgcgcccg agatggcgca
      841 ggtggccacc tcgcgccgcg ccatcacgag ccgctcccgc ccgcgcgcga attcggcata
      901 ggcctgggcc agcttcacgc cgaaggtggt gggctccgca tggatgccat ggctgcggcc
      961 gacgcagacg gtgtcgcggt gctcaagggc gcgccgcttg agcgcggcca gaagtccgtc
     1021 cacgtcggcg atgagcagat cggcggcgcg ggtcagctgg acgttgaagc aggtatcgag
     1081 cacgtcggac gaggtcatgc cctggtgcac gaagcgcgct tcgggaccca cgatctcgga
     1141 gagatgggtc aggaaggcga tcacgtcatg cttggtcacc gcctcgatct cgtcgatgcg
     1201 cgccacgtcg aagacggcat tctttccctt ctcccagatc ttcgccgccg ccgccttggg
     1261 gaccacgcca agctcggcca ggcagtcggt ggcgtgggct tcgatttcga accagatgcg
     1321 gaagcgggtc tgcggttccc agatggcagc catttcgggg cgggtatagc gggggatcat
     1381 ggcggacctt tcgtcgtctc gggcgccgga ttaacaaatc ccttggccgg gggcgagtcc
     1441 cgaagtcagt tttcccagct tggcagctcg aagggaaccg cgatgatgcg gcggttctcg
     1501 ccctcgaagc cgcggctgta gacgccgatc agcgccacct ggaggcctgg attgccaaag
     1561 cccgagggcg tgtagacggc ttcgatgtcg tagcgcagcg ggcagaggcg cgacttggga
     1621 atgctctcgt cgcgggcgat ggtgaccgat tttcccgtcg tcagcacctt cagcgtcagc
     1681 gcc
//
LOCUS       DBNGVR010000311         4780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4437581, whole genome shotgun sequence.
ACCESSION   DBNGVR010000311 DBNGVR010000000
VERSION     DBNGVR010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4437581"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16A0_06595"
     CDS             <1..326
                     /locus_tag="AC16A0_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3105037.1"
                     /translation="VALVNVAFDLTWQWQFLLFAALSVLSVYVGRAVNARSQARSSDQ
                     PHLNQRLQGFVGRRYTLAEPIKDGRGRLTIEDTVWDIAGPDSPKGAHVQVTAIDGMRL
                     IVERA"
     gene            406..1752
                     /locus_tag="AC16A0_06600"
     CDS             406..1752
                     /locus_tag="AC16A0_06600"
                     /inference="COORDINATES: protein motif:HMM:NF021589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3105038.1"
                     /translation="MAWGMVFRPTIASVLAVTGVIGVVPAAAQSTDAVPPVLQQEVDP
                     ALPDSGPVSGLPQTEPEPPPPPLRRGSDVDPYAPTGIEAGGLTLYPSITIGPVFTSNA
                     SASPDGSADAGISIGPALRIESDWVRHSYTADFEGDFDFYARNSDLKSRDVDATNTLT
                     LDVRRDTSLAFTASYVVTQSGLEDNDVPSDAVGYQTEHIVTGSAALTHGYGPLEFELA
                     AATSYNKYGDVKLQGGGTQDNSDRDYYEPSVGLRLTYVDPPVLKPFAEAAYAPRRHIE
                     TRDRNGLDRNSDGYALGAGVSIAGDPVWQGELALTYLWRSYADPSLQSQSILGLNGSL
                     TWSPTELTSIVLSFETNLDETTNPDASAARSWEIDLEATRAVQDNVNLIASGSVEISD
                     EDGPTDVTYGAGFGIEWRMSPVLAWSAAYDFTWLDAADGERNYTEHLVTAGITLSR"
     gene            complement(1774..2664)
                     /gene="galU"
                     /locus_tag="AC16A0_06605"
     CDS             complement(1774..2664)
                     /gene="galU"
                     /locus_tag="AC16A0_06605"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR3105039.1"
                     /translation="MTRPIRTAVFPVAGLGTRFLPATKAMPKEMLTVVDRPVIQLAVD
                     EAREAGIEHFVFVTGRNKGVIEDHFDKQYELESTLRKRGKSAELDLLEQDLPGAGQTS
                     FTRQQEPLGLGHAVWCARKLVGGEPFALLLPDMVMHAKPGCLRQMMDVYEARGGGNVI
                     AVEEVPWEHVHRYGVVGAKDWKGNAFAIDQMVEKPKREVAPSNLIISGRYILQPEIFA
                     EIEKQEAGAGGEIQITDAMIRLMARDRFWGVKYEGTTYDCGDKVGFLSANVAFALERD
                     DLSDSFREALKKILATHGGL"
     gene            2787..3776
                     /gene="galE"
                     /locus_tag="AC16A0_06610"
     CDS             2787..3776
                     /gene="galE"
                     /locus_tag="AC16A0_06610"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR3105040.1"
                     /translation="MSVLVTGGAGYIGSHMVLALTDAGEDVVVLDNLSTGHWWAISPK
                     ARLVEGDIGNEELLDRLMAEHRFDAVVHFAGSIVVPDSVSDPLGYYLNNPVKSRTLMA
                     CAVKAGSPRFIFSSTAAVYGNPLTMPVFEDEAPAPISPYGRSKLMTEWMLQDAHAAHG
                     LQYVALRYFNVAGADPQGRSGQSTPRATHLIKVACQTALGQRSHMDVFGTDYDTPDGT
                     CLRDYIHVTDLIAAHMDALAHLRRGGESGIFNCGYGKGYSVLEVIAAVERAHGAGFTV
                     NRVGRRAGDPAGIVAGADRVRQVLGWQPRHADLDEIVRSALDWERKLAARNAA"
     gene            3851..>4780
                     /locus_tag="AC16A0_06615"
     CDS             3851..>4780
                     /locus_tag="AC16A0_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR3105041.1"
                     /translation="MHRRTFVIALILLPLAGGPALADAKFEAFIQTLWPLAKANGISR
                     PLFDAAFEGITEPDPLVLKLANNQPEFTSTTSAYLEKAVTPIRIETGREMKEANAKLL
                     SAIEKKYGVDRHILLGIWGMESNFGKDKGSMKVMRSLATLMYSGRRKQFAREQFVAAL
                     KILKRGVVKPESFTGSWAAAMGHTQFIPTSYLSYAVDWTGDGTKDIWNSKEDALASTA
                     NYLDKAGWKNDRPWGWEVELPKNFNKALIGRKHWKTVAEWEKLGIRRADGKDFGSKSA
                     DAFVMVPQGVDGPRFLVTRNFLAIMDYNISHSYA"
BASE COUNT          849 a         1594 c         1471 g          866 t
ORIGIN      
        1 gcgtggccct ggtcaacgtg gccttcgacc tgacatggca atggcagttc cttctgtttg
       61 ccgccctgtc cgtgttgagc gtctatgtcg gccgcgctgt gaacgcgcgc agccaggccc
      121 gttccagcga tcagccgcat ctgaaccaac ggctgcaggg ttttgtcggc cgccgctaca
      181 cgctggccga acccatcaag gatggccgcg gccggctgac catcgaagac acggtctggg
      241 acattgccgg ccccgattct cccaagggcg cccatgtgca ggtcaccgcg atcgacggca
      301 tgcgcctgat cgtcgaacgc gcctgaccgg cgcgcggtcg ctcaacggca cagtccgtca
      361 acccgccatt aaccatgttg gctttagctg gccccaggtc ctggagtggc atggggaatg
      421 gtgttccgcc cgacgatcgc atctgttctg gcggtgactg gcgtcatagg cgtcgtgccg
      481 gcggctgccc agtcgaccga tgcggtgccg cccgtcctgc agcaggaggt cgatccggca
      541 ttgcccgatt cgggtccggt ttcgggcctg ccgcagaccg agcctgagcc cccgccgccg
      601 cccctgcgcc gcggcagcga tgtcgatccc tatgcgccga cggggatcga ggccggcgga
      661 ctgacgctct acccctccat caccatcggt cccgtcttca ccagcaatgc cagcgcaagc
      721 ccggacggtt cggccgatgc cggcatcagc atcggtccgg cgctgcgcat cgagagcgac
      781 tgggtgcggc attcatacac cgcggacttc gaaggggact tcgatttcta tgcccgcaac
      841 agcgatctca agtcgcgcga tgtggacgcc accaacacgc tgacactcga tgtgcggcgc
      901 gacacgtcgc tggccttcac cgccagctac gtcgtcaccc agtcggggct tgaagacaat
      961 gacgtgccgt cggatgctgt cggctaccag accgaacaca tcgtcaccgg atcggcggca
     1021 ctcacccatg gctacggccc gctcgaattc gaactggcgg cggccacgag ctacaacaag
     1081 tacggcgacg tgaagctgca gggcggcggt acgcaggaca attcggaccg cgactactac
     1141 gaaccttcgg tgggcttgcg cctgacctat gtggatccgc cggttctcaa gcccttcgcc
     1201 gaagccgcct atgcgccgcg gcggcacatc gagaccaggg accgcaatgg cctcgaccgc
     1261 aattcggatg gctatgccct gggcgccggc gtctccatcg caggcgatcc ggtctggcag
     1321 ggcgagctcg cgctcaccta tctctggcgc agctacgccg atccttccct gcaatcccag
     1381 tccattctcg gcctgaacgg ttcgctcacc tggagtccga cggaactgac cagcatcgtc
     1441 ctctccttcg agaccaatct cgacgagacc accaatccgg atgcctccgc ggcgcggagc
     1501 tgggagatcg acctggaggc cacgcgggcc gtgcaggaca atgtcaatct gatcgccagt
     1561 ggaagcgtcg agatctccga cgaggatggc ccgacggatg tcacctatgg cgccggcttc
     1621 ggcatcgagt ggcggatgag cccggttctc gcctggtccg ctgcctatga cttcacctgg
     1681 ctcgatgcgg cggacggcga gcgaaactac accgaacacc tggtgacggc gggcatcacc
     1741 ttgtcacgct gaagccggcc atcccgacag tcctcacaaa ccgccatgtg tcgcgaggat
     1801 cttcttcagc gcttcgcgga agctgtcact caggtcgtcg cgttccagtg cgaaggcgac
     1861 attggccgac aggaagccca ccttgtcgcc gcagtcatag gtggtgccct cgtacttcac
     1921 gccccagaac ctgtcacggg ccatcaggcg gatcatggca tcggtgatct ggatctcgcc
     1981 gccggccccg gcctcctgct tctcgatctc cgcaaaaatc tcgggctgca gaatgtagcg
     2041 gcccgaaatg atgaggttcg aaggcgccac ctcgcgcttc ggcttttcga ccatctggtc
     2101 gatggcgaag gcattgccct tccagtcctt ggcgcccacg acgccatagc gatgcacatg
     2161 ctcccagggc acttcttcca cggcgatgac attgccgccg ccgcgcgcct cgtagacatc
     2221 catcatctgg cgcaggcagc cgggcttcgc atgcatcacc atgtccggaa gcagcagggc
     2281 gaagggttcg ccgcccacga gcttgcgggc gcaccagacg gcatgtccga gacccagcgg
     2341 ctcctgctgg cgggtaaagc tggtctgccc ggcgcctgga agatcctgct ccagaaggtc
     2401 gagttctgcc gacttgcccc gtttgcgcag cgtcgactcc agttcgtact gcttgtcgaa
     2461 gtgatcctcg atgacaccct tgttgcgccc ggtgacgaag acgaagtgct cgattcccgc
     2521 ctcgcgggcc tcatccaccg ccagctggat gaccggcctg tccaccaccg tcagcatctc
     2581 cttcggcatg gccttggtgg ccggcaggaa gcgcgttcca agcccggcca cgggaaagac
     2641 ggctgtgcga ataggacgtg tcatgaattt accccagatt aacgccaatc atccctggca
     2701 gtcacaccgt taagcggaca tttgcatgga atggtcaaga catcccatat cagttttgct
     2761 gggatgttaa cgaaggacgg gcgggcatga gtgtcttggt cacgggtggg gcgggctata
     2821 tcggcagcca tatggttctg gccctcacgg atgccggcga agacgtcgtc gtcctcgaca
     2881 atctctcgac cggtcactgg tgggcgatct cgcccaaggc gcggctggtg gaaggcgaca
     2941 tcggcaacga agagctgctg gaccggctca tggccgaaca ccgtttcgat gcggtggtgc
     3001 attttgccgg gtccatcgtg gttccggatt ctgtcagcga tccgctgggc tactacctca
     3061 acaatcccgt gaagtcgcga accctcatgg cctgtgccgt caaggccgga agtccgcggt
     3121 tcatcttttc gtcgaccgcc gccgtctacg gcaatccgct gaccatgccg gtcttcgagg
     3181 acgaggcgcc ggcgcccatc tcgccctatg gccgctcgaa gctcatgacc gagtggatgc
     3241 tgcaggatgc ccatgccgcc catggcctgc aatatgtggc gctgcgctat ttcaacgtcg
     3301 cgggcgccga tccccagggc cggtccggcc agtcgacgcc gcgcgccacc cacctgatca
     3361 aggtagcctg ccagaccgcg ctggggcagc gcagccacat ggatgtcttc ggcaccgact
     3421 acgacacgcc cgacggcacc tgcctgcgcg actacattca cgtcaccgac ctcatcgcgg
     3481 cccacatgga tgcgctggcg catctgcgcc gcggcggcga gagcggcatc ttcaattgcg
     3541 gctatggcaa gggctattcg gtgctggagg tcattgccgc ggtcgaacgc gcccatggtg
     3601 ccggcttcac ggtgaaccgg gtggggcgcc gcgccggcga tccggccggc atcgttgcgg
     3661 gggccgaccg ggtgcgccag gttctgggct ggcagccccg ccatgccgat ctcgacgaga
     3721 tcgtgcgctc tgccctcgac tgggagcgca agctggccgc ccgcaacgcg gcctgattga
     3781 ggccgcattt ggcggctgaa ctggtctgct gttaggcttg ggcacagcct gcccgaatcg
     3841 cagagggacc atgcacagac gcacatttgt catcgccttg atcctgctgc cgctggcagg
     3901 cggcccggcg ctggccgatg ccaagttcga ggccttcatc cagacgctct ggccgctcgc
     3961 caaggccaat ggcatctcgc gcccgctgtt cgatgccgcc ttcgaaggca tcaccgaacc
     4021 cgatcccctg gtgctgaagc tcgccaacaa tcagccggag ttcacctcca ccacatcggc
     4081 ctacctggaa aaggccgtca cgcccatccg catcgagacc gggcgcgaga tgaaggaggc
     4141 aaatgccaag ctgctctctg ccatcgaaaa gaagtatggc gtcgaccgcc atatccttct
     4201 cggcatctgg ggcatggaat ccaatttcgg caaggacaag ggctcgatga aggtgatgcg
     4261 ctcgctcgcc accctcatgt actcgggccg ccgcaagcag tttgcccgcg aacagttcgt
     4321 ggccgcgctg aagatcctga agcgaggggt ggtgaaaccg gagagcttca ccgggtcctg
     4381 ggccgcggcc atgggccata cgcagttcat tccgacctcc tatctctcct atgccgtcga
     4441 ctggaccggc gacggcacca aggacatctg gaattcaaag gaagacgcat tggcctcgac
     4501 ggccaattac ctggacaagg cgggctggaa gaacgatcgt ccctggggct gggaagtgga
     4561 gctgccgaag aacttcaaca aggcgctgat cggccgcaag cactggaaga ccgtcgccga
     4621 atgggagaag ctcggcatca ggcgcgccga tggcaaggat ttcggatcga agtctgccga
     4681 tgccttcgtg atggtgccgc agggcgtcga cgggccgcgc tttctcgtga cgcggaactt
     4741 cctcgccatc atggactaca acatctcgca ctcctacgcg
//
LOCUS       DBNGVR010000312         2501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4831352, whole genome shotgun sequence.
ACCESSION   DBNGVR010000312 DBNGVR010000000
VERSION     DBNGVR010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4831352"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..421
                     /locus_tag="AC16A0_06620"
     CDS             68..421
                     /locus_tag="AC16A0_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105042.1"
                     /translation="MKRLFRYETCTEPLASRSRFTRRLLNNGLWALAIVVVAQLIGMA
                     GYLHFEGLNTIDAFTNAAMVLSGVGPTSELQTSGGKIFVSLYAVSSGLLIFAVAGLML
                     APVYHRILHRFHVAG"
     gene            486..899
                     /locus_tag="AC16A0_06625"
     CDS             486..899
                     /locus_tag="AC16A0_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HIR3105043.1"
                     /translation="MTAAATTRLIARYYAAFNAQDVEGMLACLGPGFVHDVSQGSRRR
                     GKKLFAEFLAHMNRSYKETLSNIVIMTDRTGAHAAAEFDLKGRYLVTDPGLPKAQGQT
                     YRLRVGAFFDVKGGKIVRVSTHYNMNDWKRQVLGR"
     gene            896..1483
                     /locus_tag="AC16A0_06630"
     CDS             896..1483
                     /locus_tag="AC16A0_06630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3105044.1"
                     /translation="MTIGVETLSGDDLAQGLDAVARLRIAVFRSYPYLYDGSLDYERS
                     YLEKFAAAEGAIIAVARDGDEVVGAATGCPLAAEHQAFSQPLRTLGHDVNDIFYCAES
                     VLLPQYRGRGLGHRFFDLREAQARRLGARKVAFCAVARPAGHLLRPVDYLPLDGFWRK
                     RGYAPVDGAVARFAWKDIDQPGETEKLLQFWMRQL"
     gene            1480..2376
                     /locus_tag="AC16A0_06635"
     CDS             1480..2376
                     /locus_tag="AC16A0_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3105045.1"
                     /translation="MSRPLHIAAAQYPLTEFAGLADYEAKITSWVESAAGRGAELLVF
                     PEYGAMELCSIAGRGRDLHGSIDAVSELIPELERVHSALAARHGVTIVAGSAPHRRGP
                     GHTVNVAHIYGPTGRRGSFDKIMPTPWERDPWRISGGRELKVFDIGIAKLGLLICYDI
                     EFPLLSRALAEAGAEVILAPSNTETEWGYWRVRTGAVARALENQVYTVHAPVVGSAPF
                     CLACENNTGAAGIFAPSDRGLPPGGIIALGEMNRAQWVHATVDLDLTAELRDSGGVQT
                     FKHWPEQPGAGPLPPAQLFSLV"
BASE COUNT          404 a          843 c          807 g          447 t
ORIGIN      
        1 aaccatcttg tggtcaatag tgccatgagc gagatcaatt tctgtctgcc aactccggtc
       61 cacaggcatg aaacgacttt tccgctacga aacctgcact gagccgctcg cctcgcgcag
      121 ccgcttcacc cggaggttgc tgaacaatgg cctctgggct cttgccatcg ttgtcgttgc
      181 gcaactcatc ggcatggcag gatatctcca tttcgagggc ctcaacacca tcgatgcctt
      241 caccaatgcc gcaatggtgc tgtcgggcgt ggggcctacc tccgaactgc agacctctgg
      301 cggcaagatc ttcgtgagcc tctatgcggt gtccagcggc cttctcatct tcgccgtggc
      361 cgggctcatg ctggccccgg tctatcaccg catcctgcat cgcttccacg tggccggctg
      421 agccggtccc gcttgcgctt gtcgccgcga ttggctttat gtcggcagca catcgaggag
      481 acaccatgac cgctgccgcc accaccaggc tcatcgcccg ctactacgcc gccttcaacg
      541 cccaggatgt ggagggcatg ctggcctgcc tcggtccggg cttcgtccat gatgtgagcc
      601 agggcagccg ccgcaggggc aagaagctgt tcgccgaatt cctcgcgcac atgaaccgta
      661 gctacaagga aacgctgtcc aacatcgtca tcatgacgga tcgcaccggc gcccatgccg
      721 cggccgagtt cgacctgaag ggcaggtatc tcgtcaccga tcccggcctg ccaaaggcgc
      781 aggggcagac ctacaggctc cgcgtcggtg ccttcttcga cgtgaagggc ggcaagatcg
      841 tgcgggtctc cacgcactac aacatgaacg actggaagcg tcaggtgctg ggcaggtgac
      901 gattggcgtc gagacgctgt cgggcgatga cctggcccag ggactcgatg ccgtggcgcg
      961 cctgcgcatc gccgtgttcc gcagctatcc ctatctctat gacggctcgc tggactacga
     1021 gcgcagctat ctcgagaagt tcgcggcggc cgaaggcgcg atcatcgccg tggcgcgcga
     1081 tggcgatgag gtcgtcggcg ctgccacggg atgcccgctg gcggccgagc atcaggcctt
     1141 cagtcaaccg ctcaggactc tcggccatga tgtgaacgac atcttctact gcgcggaatc
     1201 ggtcctgctg ccgcagtacc gcgggcgtgg cctgggtcat cgcttcttcg atctgcgcga
     1261 ggcccaggcg cggcgattgg gcgcgcgcaa ggtggccttc tgcgccgtgg cgcggccggc
     1321 cggtcatctg ctccgcccgg tggactatct cccgctggat ggattctggc gcaagcgcgg
     1381 ctatgctccg gtcgatggtg ccgtcgcccg cttcgcctgg aaggacatcg accagccagg
     1441 cgaaacggaa aagctcctgc aattctggat gagacagtta tgagccgccc cctccatatc
     1501 gccgccgcgc agtacccgct gaccgagttc gcggggcttg ccgactacga agccaagatc
     1561 acgtcgtggg tcgagagcgc ggcggggcgg ggcgccgaac tcctggtgtt tccggaatac
     1621 ggcgccatgg agctgtgctc catcgcaggg cgcgggcgtg acctgcacgg ttcgatcgat
     1681 gccgtgtccg agctgatacc ggaactcgag cgcgtgcatt cagcgctcgc cgccaggcac
     1741 ggggtcacca tcgtggcagg ctccgcgccg catcgccgcg gacctggcca cacggtgaat
     1801 gtcgcgcata tctacggtcc caccggccgg cgcggcagct tcgacaagat catgcccaca
     1861 ccctgggagc gcgatccctg gcggatcagc ggcgggcgcg agctcaaggt cttcgacatc
     1921 ggcatcgcga agctcgggct gctcatctgc tacgacatcg agttcccgct gctgtcgcgc
     1981 gcgctggccg aggccggcgc cgaagtcatc ctcgccccgt cgaacaccga aaccgaatgg
     2041 ggctactgga gggtgcgcac cggcgccgtg gcgcgcgcac tggagaacca ggtctatacg
     2101 gttcacgcgc cggttgtcgg ttcggcgccc ttctgcctgg cctgcgagaa caacaccggc
     2161 gccgccggaa tcttcgcgcc gtccgaccgc gggcttccgc ccggcggcat catcgccctg
     2221 ggcgagatga accgggcgca gtgggtccat gccaccgtcg atctggatct caccgcggaa
     2281 ttgcgcgact ccggtggcgt gcagaccttc aagcactggc ccgagcagcc gggcgccggc
     2341 ccgctgccgc cggcacagct gttctcgctc gtctagtcct ggtgctggat cagtgcggcg
     2401 gggaaggcac tggccagcct gcgccgcgcg gccatgccgg cggcaaggtc ggtgcagttc
     2461 acgatgccga ggcggaatcc gcccggcggc atcggatgat c
//
LOCUS       DBNGVR010000313         3174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1295937, whole genome shotgun sequence.
ACCESSION   DBNGVR010000313 DBNGVR010000000
VERSION     DBNGVR010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1295937"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="AC16A0_06640"
     CDS             <1..516
                     /locus_tag="AC16A0_06640"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3105046.1"
                     /translation="VQLGDNIMKVAEAEFAFGFSRDLPPRDHDYNLDEAMDAAAALHL
                     SIEVPDSRYADFTRVGAPSLIADTACACWLVLGPALTDGWRNLDLAAHRVSGWRNGEK
                     VAEGSGAAVLGDPRLALTWFVNEAARHCGGVKAGQFVTTGTCIVPMAIAPGDRIEIDY
                     GALGKIGAVIA"
     gene            complement(506..1795)
                     /gene="guaD"
                     /locus_tag="AC16A0_06645"
     CDS             complement(506..1795)
                     /gene="guaD"
                     /locus_tag="AC16A0_06645"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HIR3105047.1"
                     /translation="MTRVIRGQTLSFGASPDEARHEAKGAIAVGDDGSILWAGPAPLL
                     PQGFRQAPVDDYGDCIVMPGFVDAHIHFPQCRMLAAPGKDLLDWLSRFTFPEESRYAD
                     GGYAAAMAQLFLDRLAAHGTTSAMAFCSVHKACADALFAAAASRNMALVTGKTMMDRN
                     AIPAVQDTPETGARESVELYRAWHGKGRLRYAVTPRFAITSSEAQLRVSGELLQSLDG
                     VLMQTHLSESPGEIALVRELFPNARDYTDVYDRFGLLGSRSFFAHGIHLSEAECARLS
                     ESGSTVVHCPTSNNFLGSGLMRMAYLREARRPVNVGLATDVGGGTTYSMLATLGETYK
                     VQMLTGYKPSALELFRMATRGNAERLRLPETGAIDVGWHADLVVLDPCATPVLASRHE
                     LSSSLEDVLFALMILGDDRAVRATYIAGRKLHERVTQ"
     gene            complement(1792..2289)
                     /gene="uraD"
                     /locus_tag="AC16A0_06650"
     CDS             complement(1792..2289)
                     /gene="uraD"
                     /locus_tag="AC16A0_06650"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000255 - allantoin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HIR3105048.1"
                     /translation="MMTIADVNALAPVDFIARFGDVAEHSPWVAREAFAQRPFADRDA
                     MVEAFAMALKSANPDAQRALIRAHPDLATKARLTADSSREQAGAGLDALSEEEFVRFT
                     KLNSAYKDRFGFPFIFAVKGATKQMVLAAFEERVSHSPEAEFATAIAQICRIFRFRIE
                     DRVAA"
     gene            complement(2286..>3174)
                     /locus_tag="AC16A0_06655"
     CDS             complement(2286..>3174)
                     /locus_tag="AC16A0_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR3105049.1"
                     /translation="LYYFQAETYLIDPSVMPLTRWQAVGISVGSLAAGWLLYSALCRS
                     PLGRNAGLLGTLVFALILGAAWFYVHVFSGRGAFIHVGALIGTIMAANVFMVIIPNQR
                     RITAALLKGETPDPQLGAIGKQRSLHNTYLTLPVLLMMISNHYPMITDHAQAWILAGL
                     VVLGGVLLRHFLVRIEVGDPQAEIAWTLPLIGLALAAALFMTQPEARTVFEGEVTDAE
                     ALAIVQARCATCHAAVPTDPTIKAPPKGIALDTLEKLKRYAAQVETQSVKTKAMPLGN
                     KTGMLPEERAKLGAWIARQ"
BASE COUNT          549 a         1014 c         1093 g          518 t
ORIGIN      
        1 gtccagctcg gcgacaacat catgaaagtg gccgaggcgg agtttgcctt tggcttctcg
       61 cgcgacctgc cgccgcgtga ccatgactac aacctcgacg aggccatgga cgccgcggca
      121 gcgctgcatc tctccatcga agtcccggac agccgctacg ctgacttcac cagggtcggt
      181 gcgccgtcgc tcatcgccga caccgcctgt gcctgctggc tggtgctggg ccctgccctc
      241 accgatggct ggcgcaacct cgatctcgca gcgcatcggg tgtccggctg gcgcaacggc
      301 gagaaggtcg ccgagggaag cggcgcggcg gtgctgggcg atccgcgcct tgcgctgacc
      361 tggttcgtca acgaggccgc gcgccattgc gggggcgtga aagccggcca gttcgtcacc
      421 accggcacct gcatcgtgcc catggccatt gcacccggcg atcgcatcga gatcgactat
      481 ggcgcgctgg gcaagatcgg cgccgtcatt gcgtgacccg ttcgtgcagc ttgcgccccg
      541 cgatgtaggt ggcgcgaacc gcccggtcgt cgcccaggat catcagcgca aagagcacgt
      601 cctcaagcga gcttgagagt tcgtgacggc tggcaaggac cggcgtggcg caggggtcca
      661 gcacgacaag atcggcatgc cagccgacgt cgatggcgcc tgtctccggc aatcgcaggc
      721 gctcggcatt gccgcgcgtg gccatgcgga acagctccag cgcactgggc ttgtagcccg
      781 tgagcatctg gaccttgtag gtctcaccca aggtcgcgag catcgaatag gtcgtgcccc
      841 cgccaacatc ggtggcgagg ccgacattaa ccggcctccg ggcctcgcgc agataggcca
      901 tgcgcatgag gccggagccg aggaagttgt tggacgtcgg gcaatgcacc acggtcgacc
      961 cgctctcgga tagccgcgcg cattccgctt ccgacaggtg aatgccatgc gcgaagaagc
     1021 tgcgtgaccc aagcaggccg aagcggtcgt agacgtcggt atagtcgcgc gcgttgggga
     1081 agagttcgcg caccagtgcg atctcgccag ggctttccga caggtgcgtc tgcatcagca
     1141 caccgtcgag ggactgcagc aattcgcccg agacgcgaag ctgcgcctcg cttgaggtga
     1201 tggcaaagcg cggcgttacc gcataacgca gccgcccctt gccatgccag gcgcgataga
     1261 gctccacgct ttcgcgcgcg cccgtttcgg gcgtgtcctg aacggcggga atggcattgc
     1321 ggtccatcat cgtcttgccg gtgaccagcg ccatgttgcg cgaagctgcg gccgcgaaga
     1381 gcgcatcggc gcaggccttg tgcaccgagc agaaggccat ggccgacgtc gtgccatggg
     1441 cggcgaggcg gtcgaggaag agttgggcca ttgcggcggc atagccgcca tcggcatagc
     1501 ggctttcttc cgggaaggtg aagcggctca gccagtccag caggtccttg ccgggcgcgg
     1561 ccagcatgcg gcactgtggg aagtggatgt gcgcgtcgac gaaacccggc atgacgatgc
     1621 agtcgccata gtcgtcgacc ggcgcctggc ggaagccctg cggcagcagc ggtgccggcc
     1681 cggcccagag gatgctgccg tcgtcgccga cggcgatggc gcccttcgcc tcatggcggg
     1741 cctcgtccgg cgaggccccg aagctcagcg tctggccgcg gatcacgcgc gtcatgcggc
     1801 cacccggtct tcgatgcgga agcggaagat gcggcagatc tgcgcgatgg ctgtggcgaa
     1861 ttcggcttcc ggcgaatgcg agacccgttc ctcgaaggcc gcaagaacca tctgcttggt
     1921 cgcgcccttg accgcgaaga tgaagggaaa gccgaacctg tccttgtagg ccgaattgag
     1981 cttggtgaag cgtacgaatt cctcctccga caaggcgtcg agaccggcgc ccgcctgctc
     2041 gcggctgctg tccgccgtca gcctggcctt ggtcgcgaga tcggggtgcg cccggatcaa
     2101 ggcccgctgg gcatccgggt tggcagactt cagcgccatg gcgaaggctt caaccatggc
     2161 gtcacggtcc gcgaaggggc gctgggcgaa ggcttcgcgc gccacccagg gtgagtgttc
     2221 cgccacgtcg ccgaagcgcg caatgaagtc cacgggggcc agggcattca catctgcgat
     2281 tgtcatcatt ggcgtgcaat ccatgcgccg agcttggcgc gttcctcggg cagcataccg
     2341 gtcttgttgc caagcggcat ggccttggtc ttgaccgact gggtttcaac ctgcgcggca
     2401 tagcgcttca gcttctccag cgtatcgagc gcgatgccct ttggcggcgc cttgatggtg
     2461 ggatcggtag ggacggccgc gtggcaggtt gcacaacgcg cctgaacgat ggcgagcgcc
     2521 tcggcgtctg tcacctcgcc ctcgaagacc gtccgcgctt cgggttgagt catgaagaga
     2581 gcggccgcca gcgcaaggcc gatcagcggc aaggtccagg cgatctccgc ctgcgggtcg
     2641 cccacctcga tgcgcaccag gaagtgtcgc agcaggacgc cgcccagaac cacgagcccc
     2701 gccagaatcc aggcctgggc atgatcggtg atcatcggat agtggttgct gatcatcatc
     2761 agcagcaccg gtagcgtgag ataagtattg tgcagcgagc gctgcttgcc gatggcgcca
     2821 agctgcgggt ccggcgtctc gcccttgagc agcgcggcag tgatccggcg ctggttggga
     2881 atgatcacca taaagacgtt ggccgccatg atggtgccga tcagcgcgcc gacatggatg
     2941 aaggcgccac gccccgagaa gacatgcacg tagaaccagg ccgccccgag gatgagcgcg
     3001 aagacgagcg tgccgagcag gccggcgttg cggcccagcg gcgagcggca cagggcgctg
     3061 tagagcagcc agcctgcggc aaggctgccg acggagattc cgaccgcctg ccagcgggtg
     3121 agcggcatga cggacggatc gatcaggtag gtctccgcct ggaaatagta gagc
//
LOCUS       DBNGVR010000314         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3652912, whole genome shotgun sequence.
ACCESSION   DBNGVR010000314 DBNGVR010000000
VERSION     DBNGVR010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3652912"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="AC16A0_06660"
     CDS             <1..1251
                     /locus_tag="AC16A0_06660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3105050.1"
                     /translation="MIGRLFRRVTLISAARLDWVMPDRVAYLAGLILAAVVFWAIGSG
                     VILTGALRFLDNTYRRVDAFLPPEMAAPIDPNKTGSPQSLISWDSLGAQGRNRVVAAP
                     TRADIEALTGGPAMEPLRIYVGANSADTAEDRAALALAELIRTGAFDRSVLVIATPTG
                     TGWVDPSGMTPVEFLHRGDIASVSVQYSYLPSWLSLLVEPEYGHETAEAVFRTVYGHW
                     RKLPADSRPRLYLFGLSLGSLNSDLAADFFDIVAEPYHGALWAGPPFASRTWNKVTAE
                     RVPDTPAWLPRFRDSSLFRFTGQTNHLDIPGASWGPMRMVYLQYASDPITFFEESSLW
                     RPPAWMKSPRGPDVSPRLVWVPVVTFLQLICDMMTATQTPRGKGHVYAATHYLDGWVA
                     VTSPAGWTESDLARLRTWMADRDL"
     gene            complement(1257..>2675)
                     /locus_tag="AC16A0_06665"
     CDS             complement(1257..>2675)
                     /locus_tag="AC16A0_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011428246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR3105051.1"
                     /translation="PTIQSALGIAPDRMSWIQTAYLIAEIIAIPLTGFFTRLLGMRLL
                     FMSATAVFTLASVGCAASGSFEVLIFWRVIQGFAGGALIPAVFSAVFLFFPVNRQGLA
                     TSIAGVIAVLAPTLGPSIGGWVTEAWSWHWLFLINLLPGIAAAVAVFFLLPRRTVAWS
                     ELRRLDIVSLLLLAVALAALEIGLKEAPKSGWLSGITLALLALSVISAVSFVGRSLRG
                     VHPIVDLKLFRDRNFSIACLLSFLLGIGLFGSVYLMPVFLAFVRDHGALQIGLIMLVT
                     GVTQLLFAPLAVQFDRRIDARLLSGIGFLLFGLGLGWSAFQTPETDFDGMFWPQVLRG
                     AAIMFCLLPPTRLALGELEKLRVPDASGVFNLMRNLGGAIGIALIDTIVFGRAPDHGE
                     ALLAQLQAGDVGAADFVGVPLDEFQALSQGSIDVEAIGMLQTAIEKAAMTMAVNEAWA
                     VLSVMTLAAVLLLPAARKPSQI"
BASE COUNT          539 a          879 c          816 g          441 t
ORIGIN      
        1 atgatcgggc ggctgtttcg ccgcgtcacc ctcatctccg ccgcgcggct ggattgggtc
       61 atgcccgacc gtgtcgccta tctcgccggc ttgatcctgg ctgccgttgt cttctgggca
      121 atcggcagcg gcgtgattct gaccggcgcc ctgcgcttcc ttgacaacac ctaccgccgt
      181 gtcgatgcct ttctgccgcc cgagatggct gcgccgatcg atccgaacaa gaccggcagc
      241 cctcagtcgc tgatctcctg ggacagtctt ggcgcccagg ggcgcaaccg tgtcgtcgcc
      301 gctcccaccc gcgccgacat cgaagcgctg accggcggtc cggcaatgga gcccctgcgg
      361 atctatgtgg gcgccaattc ggccgacacg gccgaagacc gggccgcact ggcgctggca
      421 gaactgatcc ggaccggcgc ctttgaccga tcggtgctgg tcatcgcgac gccgacggga
      481 acaggctggg ttgacccgtc cggcatgacg ccagtcgagt tcctgcaccg cggcgacatc
      541 gcgtcggtct ccgtgcagta ctcctacctt cccagttggc tgtcgcttct cgtcgaaccg
      601 gaatacggtc acgagacggc ggaagcggtg ttccgcacgg tttacggcca ctggcgcaag
      661 ctgcccgccg attcccggcc ccggctctat ctcttcggac tgagtctcgg ttcgctgaat
      721 tccgatctgg cggccgactt cttcgacatc gtggccgaac cttaccacgg cgcgctgtgg
      781 gccggcccgc catttgccag ccgcacatgg aacaaggtca cggcagaacg cgtgcctgac
      841 acgccggcgt ggttgcccag attccgcgac agctcgctgt tccgcttcac cggccagacc
      901 aatcatctcg acattcccgg cgcaagctgg ggaccgatgc ggatggtcta tctgcagtac
      961 gcatccgatc ccatcacctt cttcgaggag agcagtcttt ggcggcctcc ggcctggatg
     1021 aagtcgccgc gcggccccga tgtatctccg aggctggtct gggtaccggt ggtgaccttc
     1081 ctgcagttga tctgcgacat gatgacggca acgcagactc cgcgcggcaa ggggcatgtc
     1141 tatgccgcca cgcactacct cgacggctgg gtggcggtca cctcgccggc cggatggacg
     1201 gagtccgatc tcgccaggct caggacctgg atggcagacc gcgatctgtg aaggcctcag
     1261 atctgcgatg gcttgcgtgc cgcgggcagc agcagcacgg ccgcaagcgt catcaccgac
     1321 agcaccgccc aggcctcgtt gacggccata gtcattgcag ccttttcgat ggctgtctga
     1381 agcatcccga tcgcctcgac gtcaatggag ccttgcgaga gggcctgaaa ctcgtcgagc
     1441 ggaacgccaa cgaaatctgc cgctcccaca tcaccagcct gtagctgcgc cagcaaggct
     1501 tccccgtggt ccggcgcacg gccgaaaaca atggtgtcga tgagggcgat gccaatggcg
     1561 ccgcccagat tgcgcatcag gttgaagact ccgctcgcat ccggaacgcg caacttctcg
     1621 agctcaccca gtgccagccg cgtcggcggc aagaggcaga acatgatcgc agccccacga
     1681 aggacctgcg gccagaacat tccgtcaaag tctgtttcag gggtctgaaa ggcgctccag
     1741 cccaatccaa gaccgaacaa caggaacccg atgcccgaca ggaggcgtgc gtctatccgc
     1801 cgatcgaact gcactgccaa tggtgcaaac aagagctgcg tgacaccggt caccagcatg
     1861 atcagcccga tctgcaacgc cccgtggtcg cgaacgaagg cgagaaagac cggcatcagg
     1921 taaacggatc cgaagaggcc gatgccgagc aggaagctca agaggcaggc gatgctgaaa
     1981 ttgcgatcac ggaagagctt gaggtcaacg atgggatgaa cccctcgcaa cgatcttccg
     2041 acgaagctga ccgccgagat caccgacagg gcaagcagag ccaaggtgat tccggacagc
     2101 catccgctct tcggtgcttc cttcagaccg atctcaaggg cagccagcgc aacggcaaga
     2161 agcaagagcg agacgatgtc gagcctgcgc aactcgctcc acgccaccgt ccttcgcggc
     2221 aggaggaaaa agacggcaac cgcggcagcg atgccgggca agaggttgat caggaacagc
     2281 caatgccacg accacgcctc cgtcacccag ccgccgatgc ttggtcccag agtcggcgcg
     2341 agaacggcga tcacgcctgc aatgctggtg gccagcccct gccggttgac agggaagaag
     2401 aggaacacgg ccgaaaagac agcaggaatc aacgctccgc ctgcgaagcc ctgaatcacc
     2461 cgccagaaga tcagaacttc gaaactgccg cttgccgcgc acccaactga cgccagcgtg
     2521 aagacggcag tagcggacat gaagagcagt ctcatgccca gaagccgtgt gaagaagccg
     2581 gtgagcggaa tggcgatgat ttcggcgatc agataggccg tctggatcca gctcatgcgg
     2641 tccggcgcga tgccaagcgc tgactgaatc gtcgg
//
LOCUS       DBNGVR010000315         7066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3653143, whole genome shotgun sequence.
ACCESSION   DBNGVR010000315 DBNGVR010000000
VERSION     DBNGVR010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3653143"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /gene="leuB"
                     /locus_tag="AC16A0_06670"
     CDS             complement(<1..936)
                     /gene="leuB"
                     /locus_tag="AC16A0_06670"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3105052.1"
                     /translation="MASYKLLLLPGDGIGPEVMAEAEKLIAWFNAQGIARFETERGLV
                     GGSAYDAHGKAISESDMALAMASDAVLFGAVGGPKWDKVPYEVRPEAGLLRLRKDLGL
                     FANLRPAICYPALADASSLKREVVEGLDILIVRELTGGVYFGEPKTITDLGNGQKRGI
                     DTQVYETYEIERISRVAFELALTRRNKVSSAEKRNVMKTGVLWNEVVTRIHAAEYPKV
                     TLEHVLADNCAMQLVRWPKQYDVIVTDNLFGDVLSDVAAMLTGSLGMLPSASLGAPDA
                     KSGRRKSLNEPVHGSAPDIAGTGAAKPIAMIASVGM"
     gene            complement(1007..1372)
                     /locus_tag="AC16A0_06675"
     CDS             complement(1007..1372)
                     /locus_tag="AC16A0_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105053.1"
                     /translation="MLKKIRLELARDHDHPEGSNRIGYEFGAPLDAAGRIDADEWHRL
                     RDHCRVRRFRPGEEDDIGHLIRKPGGSWAFHYDIHSDEEDDESGYRFADHVFKSGEYV
                     SIREDDGLRTFRVTRVQDA"
     gene            complement(1382..2224)
                     /locus_tag="AC16A0_06680"
     CDS             complement(1382..2224)
                     /locus_tag="AC16A0_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3105054.1"
                     /translation="MGYKTILVSLNEVGRLPQLLAAATKLGQALGTHVSGLYVIPAVQ
                     VYPSVGFEAAPQVFEGNRSFFKDNAAKVREQFEAAMTKEGLAFDFHQVDSRTPIIADE
                     VVAKGRCADVLVVSTTNPDEVTGVERDFVEQVLMGVGRPVVVLPHQGEAKLSLDDVVV
                     GWDGGREAARSTFDALPLLRVAKKVRVIRVDPQKDASLKASVPGADIAETLARHGVKV
                     ETQGYPTDGLDEGQALLRCADDVGAGLIVMGAYGHSRLAEFIFGGATRFVLSRLNRPV
                     LMSH"
     gene            complement(2289..3041)
                     /locus_tag="AC16A0_06685"
     CDS             complement(2289..3041)
                     /locus_tag="AC16A0_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3105055.1"
                     /translation="MHGTVTSILPDVMHRCDHCSVRHQAVCGAMDEVQIKKLAQIAHR
                     KKVSAGQTIISDEEPVDFFANVISGAIKLTKTLPDGRQQIVGLLFAPDFLGRAYSKNN
                     PYTAEAATDVEICTFPNAAFERLVGEYPGLQQRLFQHTLDELDAARDWMLLLGRKTAE
                     EKVASFLYMLARRSLMTGCQHKNTPGMSGFELPLTRADIADYLGLTIETVSRQLTRLK
                     TSHVIRLSSNRLILVPDMAKLAQFAGQDSLHH"
     gene            complement(3132..4442)
                     /gene="preA"
                     /locus_tag="AC16A0_06690"
     CDS             complement(3132..4442)
                     /gene="preA"
                     /locus_tag="AC16A0_06690"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020051316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HIR3105056.1"
                     /translation="MADLRSTFVGITSPNPFWLASAPPTDKEYNVVRAFKAGWGGVVW
                     KTLGEDGPPIVNVNGPRYGAIHGADRRLLGLNNIELITDRPLEVNLREIKKVKREWPD
                     RAMIVSLMVPCNEEAWKAILARVEETDCDGIELNFGCPHGMSERGMGAAVGQVPEYIE
                     MVARWCKQHSRMPVIVKLTPNITDIRYPARAAMKGGADAVSLINTISSIVSVDLDNFA
                     PMPTIDGKGSHGGYCGPAVKPIALNMVAEIARDKETHGLPISAIGGISTWRDAAEFIA
                     MSAGTVQVCTAAMTYGFRIVEEMKSGLARWMDEKGYATIEDFRGRAVPNVTDWQYLNL
                     NYVTKAHIDQDLCIKCGRCYAACEDTSHQAIMKEKDGKRHFEVIDAECVACNLCVEVC
                     PVQDCITMVRMTEGTDPRTGRKIEPSYANWTTHPNNPMALKAAE"
     gene            complement(4454..5791)
                     /locus_tag="AC16A0_06695"
     CDS             complement(4454..5791)
                     /locus_tag="AC16A0_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3105057.1"
                     /translation="MAAGKGPDISGKRLTRQDYAANFADLHPPLNDHEALVEADRCYF
                     CHDAPCMTACPTSIDIPMFIREIQAGHPKSAAKTIFDQNILGGMCARVCPTETLCEEA
                     CVREAAEGEPVKIGQLQRYATDIAMQDKIHPYVRAMASGKRVAVVGAGPAGLACAHRL
                     AMHGHDVTIYEARKKSGGLNEYGIAAYKTVDNFAQKEVDFVQAIGGIAIEHGKALGKK
                     LKLVDLQKHYDAVFLGMGLQGVNALAAKDEDADGVVDAVDYIADIRQARDLSRLPVGR
                     RVLVIGGGMTAIDVAVQSKLLGAEDVTILYRRGQEQMKASLFEQDLAQTRGVKIKHWV
                     MPKKVLIKKGQVVGLRCEYTVEKKGRLEGTGETFDLPADMIFKAIGQTFVDAAGGALE
                     LSGGRIKVDAERRTSVQGIWAGGDCAAGGKDLTVAAVDDGRRAAESINAFLKA"
     gene            5987..>7066
                     /gene="ccrA"
                     /locus_tag="AC16A0_06700"
     CDS             5987..>7066
                     /gene="ccrA"
                     /locus_tag="AC16A0_06700"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HIR3105058.1"
                     /translation="MTANAKAAKPANTNAPVKGLYEIGEIPPMGHVPEKMYAWCIRQE
                     RHGAPDTAMQIEVVPTWSIGEDECLVLVMAGGVNYNGIWAALGKPISPHDGHKNPYHI
                     AGSDASGIVYAVGAKVKRWKVGDEVVIHCNQDDGDDEECNGGDPMFSNSQRIWGYETP
                     DGSFAQFTRVQARQLLPKPQHLTWEEAACYTLTLATAYRMLFGHRPHVLSAGQNVLVW
                     GASGGLGSMAIQLIATVGANAIGVISDDDKTDFVKQLGARGVINRKNFNCWGQMPQVG
                     TPAYNEWLKNAREFGKAIWAFTGKGNNVDMVVEHPGESTFPVSVLMTRRGGMVVICAG
                     TTGFNLTMDARYLWMHQKRVQGSHFA"
BASE COUNT         1245 a         2283 c         2214 g         1324 t
ORIGIN      
        1 catgccgact gaagcgatca tggcgatcgg ttttgccgca ccggtgcccg cgatgtcggg
       61 cgcagagcca tgcaccggct cgttgagcga cttgcggcgg ccgctcttgg cgtcgggggc
      121 tccgagcgag gccgagggca gcatgccgag cgagccggtg agcatggcgg ccacatccga
      181 aagcacgtcg ccgaacagat tgtcggtgac gatcacgtcg tactgcttgg gccagcgcac
      241 cagctgcatg gcgcaattgt cggccagcac gtgttccagc gtcaccttgg ggtattcggc
      301 cgcgtggatg cgggtgacca cctcgttcca caataccccc gtcttcatca cgttgcgctt
      361 ttcagccgag gagaccttgt tgcggcgggt cagtgccagc tcgaaggcga cgcggctgat
      421 gcgctcgatc tcgtaggtct cgtagacctg cgtgtcgatg ccgcgcttct gcccgttgcc
      481 caggtcggtg atggtcttgg gttcgccgaa gtagacgccg cccgtcagtt cacggacgat
      541 caggatgtcg agcccttcca cgacctcgcg cttgagcgag gaagcatccg ccagcgccgg
      601 atagcagatg gcggggcgca gattggcgaa gaggccgaga tccttgcgca gccgcaacag
      661 tcccgcctcg gggcgaacct cgtaaggcac cttgtcccac ttgggcccgc ccaccgcgcc
      721 gaagagcacg gcgtcggagg ccatggcaag ggccatgtcg gattcggaga tggccttgcc
      781 atgggcgtca tatgcggagc cgccgaccag tccgcgctcg gtctcgaagc gcgcgatgcc
      841 ctgggcattg aaccaggcga tgagcttctc ggcttcagcc atgacttccg ggccgatgcc
      901 gtcgcccgga agcagaagga gtttatagga ggccatgtgc taatccatgc gtgagggaaa
      961 accggcgcga tgggtagccc ttggcgaagg ccggcgcaag ccggatttat gcgtcctgga
     1021 ccctggtcac gcggaaggta cgcaggccat cgtcctcgcg gatcgacacg tactcgccag
     1081 atttgaagac atgatcagcg aagcggtagc cgctctcgtc atcttcctcg tccgagtgga
     1141 tgtcgtaatg gaaagcccag gagccgcccg gcttgcggat cagatggccg atgtcatcct
     1201 cctcgccggg gcggaagcgc cgcacccggc agtgatcccg cagcctgtgc cactcgtcgg
     1261 catcgatgcg gcctgccgca tccagcggcg cgccgaattc atagccgatc ctgttggagc
     1321 cttcgggatg atcgtggtcg cgggccagtt caagtcggat cttcttcagc atgccatcac
     1381 ctcaatgcga catgagaacc ggccggttga gccggctcag cacgaatcgg gtggcgccgc
     1441 cgaagatgaa ctcggccaac cggctgtggc cataggcgcc catgacgatc aggcctgcac
     1501 caacatcatc ggcacagcgc agaagcgcct ggccttcatc gagaccatcc gtcggatagc
     1561 cctgggtttc caccttcacg ccatgacgcg ccagagtttc ggcaatgtcg gcccccggca
     1621 cgctggcctt cagactggca tccttctgcg gatcgacccg gatcacgcgc actttctttg
     1681 cgacccggag cagcggcagc gcatcgaagg tcgagcgggc cgcctcgcgg ccgccgtccc
     1741 atccaaccac gacgtcatcc agcgacagct tcgcctcgcc ctgatgaggc agcaccacca
     1801 cggggcgtcc gacgcccatc agcacctgct cgacgaagtc gcgctcgacg ccagtgacct
     1861 cgtccggatt ggtggtggaa accacgagga cgtcggcgca tcgacccttg gccaccactt
     1921 cgtcggcaat gatcggcgtc cggctgtcaa cctgatggaa gtcgaaggca agtccctcct
     1981 tggtcatcgc ggcttcaaac tgctcgcgca ccttggcggc attgtccttg aagaagctgc
     2041 gattgccttc gaagacctgg ggagcggcct cgaagcccac ggaagggtag acctgcaccg
     2101 cgggaatgac atagagaccg gacacatgcg tccccagcgc ttgccccagc ttggtcgcgg
     2161 cagcaagcag ctgcggcagc cggccgacct cgttgaggga caccagtatc gtcttgtaac
     2221 ccatcgcttt atcctcctgt gtcgggcggg atagtggccg aagggatcag ggcgagcctt
     2281 gaccccgatc aatgatgcag tgaatcctgc cccgcgaact gggccagctt cgccatgtcc
     2341 ggcaccagga tcaaccggtt cgaactcagc cgtatgacat ggctggtctt gagccgggtc
     2401 agctggcggc tcacggtttc gatggtgagg ccgagatagt cggctatgtc ggcccgggtc
     2461 agcggcaatt cgaagcccga catgccgggc gtgttcttgt gctggcagcc ggtcatgagg
     2521 ctgcggcgcg ccagcatgta gaggaagctc gcaaccttct cctcggctgt cttgcggccc
     2581 agcagcagca tccagtcgcg ggccgcatcg agctcgtcca gcgtgtgctg gaacaggcgt
     2641 tgctgcagcc cggggtactc gccgaccaga cgttcgaagg cggcattggg gaaggtgcag
     2701 atctcgacat cggtcgccgc ttccgccgta tacggattgt tcttgctata ggcccgccca
     2761 agaaagtccg gcgcaaagag aaggccaacg atctgctggc ggccatcggg caaggtcttg
     2821 gtcagcttga tggcacccga gatcacattg gcgaagaagt cgaccggttc ttcatccgag
     2881 atgatggtct gaccggcgct gaccttcttg cgatgggcga tctgcgcgag cttcttgatc
     2941 tggacttcgt ccatggcgcc gcagacggcc tgatgccgga ccgagcagtg atcgcagcga
     3001 tgcatcacat cgggcaggat ggacgtgact gttccatgca tgacgcctgt atcccccgtt
     3061 tctgccatga ttgtagatgt agtgtctctc tcccggaatt gggctgcgtc ggatcgtccc
     3121 gtcctgcctt actactccgc ggccttcagc gccatggggt tgttcggatg ggtggtccaa
     3181 ttggcatagg agggttcgat cttccggccg gtccggggat ccgttccttc ggtcatgcgg
     3241 accatggtaa tgcagtcctg caccggacac acctccacgc agaggttgca ggccacgcac
     3301 tcggcgtcga tcacctcgaa gtgccgttta ccatcctttt ccttcatgat cgcctggtgc
     3361 gaagtgtctt cgcaggcggc atagcagcgg ccgcacttga tgcagagatc ctggtcgata
     3421 tgcgccttgg tcacgtagtt gagattgaga tactgccagt cggtgacgtt gggcaccgcg
     3481 cggccgcgga agtcctcgat ggtggcatag cctttctcgt ccatccagcg cgccagaccg
     3541 gacttcatct cctcgacgat gcggaagcca taggtcatcg cggcagtgca gacctgcacc
     3601 gtgccggcgg acatggcgat gaattccgcc gcatcgcgcc aggtcgagat cccgccgatg
     3661 gccgagatcg gaagcccgtg ggtttccttg tcgcgcgcga tctccgccac catgttgagc
     3721 gcgatgggct tgaccgccgg tccgcaatag ccgccatggc tgcccttgcc gtcgatggtg
     3781 ggcatgggcg cgaaattgtc gagatcgacc gaaacgatcg acgagatcgt gttgatcagc
     3841 gacacggcat cggccccgcc cttcatcgcg gcccgcgccg gatagcggat atcggtgatg
     3901 ttgggcgtga gcttgacgat caccggcatg cgcgagtgct gcttgcacca gcgcgccacc
     3961 atctcgatgt attcgggcac ctgccccacg gcagccccca tgccgcgctc gctcatgcca
     4021 tgcgggcagc cgaagttcag ttcgatgccg tcgcagtcgg tctcctcgac acgggccagg
     4081 atcgccttcc aggcctcctc gttgcacggc accatcagcg acacgatcat ggcgcggtcg
     4141 ggccactccc gcttcacctt cttgatctca cgcagattga cctcgagcgg ccggtcggtg
     4201 atcagctcga tgttgttgag cccgagcagg cgccggtcgg cgccgtggat ggcgccataa
     4261 cgcggaccat tgacgttgac gatgggcggg ccgtcctcgc ccagcgtctt ccacaccacg
     4321 ccgccccagc ccgccttgaa ggcgcgcacc acgttgtact ccttgtcggt cggcggcgcc
     4381 gaggccagcc agaaaggatt gggcgacgtg atgccgacga aggtggagcg aagatcagcc
     4441 atggtccctc tcctcaagcc ttgagaaacg cgttgatgga ttcggccgca cggcggccat
     4501 cgtcgactgc cgcaaccgtg aggtccttgc cgcccgcagc gcaatcgccg ccggcccaga
     4561 tgccctggac actggtgcgg cgctcggcat caaccttgat gcgcccgccc gacagttcga
     4621 gcgcgccgcc ggccgcatcg acgaaggtct ggccgatggc cttgaagatc atgtcggcgg
     4681 gaagatcgaa ggtctcgccc gtgccttcga gcctgccttt cttctccacc gtgtactcgc
     4741 agcgcagccc cacgacctgg cccttcttga tcagaacctt cttcggcatg acccaatgct
     4801 tgatcttcac gcccctggtc tgggccagat cctgttcgaa gaggcttgcc ttcatctgct
     4861 cctggccgcg gcgatagagg atggtcacgt cctccgcgcc cagcagtttc gattggaccg
     4921 cgacgtcgat ggcagtcatg ccgccgccga tcaccagcac gcggcgcccc acaggaagcc
     4981 tggacaggtc tctcgcctgg cggatgtcgg cgatatagtc gaccgcatcg acgacgccgt
     5041 ccgcatcttc gtccttggcg gccagcgcat tcacgccctg caggcccatg ccgagaaaca
     5101 ccgcatcata atgcttctgc agatcgacaa gcttcaactt cttgcccaag gccttgccat
     5161 gctcgatggc gatgccgccg atcgcttgca cgaagtccac ttccttctgc gcgaagttgt
     5221 cgaccgtctt gtaggcggcg ataccatact cgttgaggcc gccggatttc ttgcgcgcct
     5281 cgtagatggt gacatcatgt ccatgcattg ccagccggtg ggcgcaggcc agcccggccg
     5341 ggccggcgcc gacaaccgcc acgcgcttgc cgctcgccat cgcccgcaca tagggatgga
     5401 tcttgtcctg catggcgata tcggtggcat agcgctgcaa ctggccgatc ttcacgggct
     5461 cgccctctgc ggcctcgcgg acacaggcct cttcgcacag cgtttcggtc gggcagaccc
     5521 tggcgcacat gccgcccagg atattctggt cgaagatcgt cttggccgcg ctcttcgggt
     5581 gacccgcctg gatctcgcga atgaacatgg ggatgtcgat ggaggtcggg caggccgtca
     5641 tgcagggcgc atcgtggcag aagtagcagc ggtcggcttc gaccagggcc tcatggtcat
     5701 tgaggggcgg gtgcaggtcg gcgaagttcg cggcgtagtc ctgcctggtg agccgtttgc
     5761 ccgagatatc cgggcccttg cctgctgcca tcggggcatc ctccagacgc attgcgctaa
     5821 gtgtcgtgga cgatgacaga ttttgttcaa acggtcaaag tccactgccg ttcgggcact
     5881 tagtggccag ctcccgaaaa actcgacctt cgtcgagagc gggcttttgt tgcattgcaa
     5941 gatcgtctag ctttctgcca cattgcccgt tcaaggggag agaaacatga cggcaaacgc
     6001 caaggctgcg aagcctgcca atacaaatgc gcccgtaaag ggcctctacg aaatcggcga
     6061 gatcccgccg atgggtcacg tgccggaaaa gatgtatgct tggtgcatcc ggcaggaacg
     6121 gcacggcgcc ccggacacgg ccatgcaaat cgaggtggtt cccacctggt cgatcggcga
     6181 ggacgagtgc ctggttctgg tgatggccgg cggggtcaac tacaacggca tctgggccgc
     6241 gctcggcaag cccatttcgc cgcacgacgg gcacaagaac ccttaccaca tcgcgggctc
     6301 ggatgcctcg ggcatcgtct atgccgtggg cgcgaaggtg aagcgctgga aggtaggcga
     6361 cgaggtcgtc atccactgca accaggacga cggcgacgac gaggaatgca atggcggcga
     6421 cccgatgttc tccaactcgc agcgcatctg gggctacgaa accccggacg gatccttcgc
     6481 gcagttcacc cgcgtccagg cccgccagct gctgcccaag ccccagcatc tgacctggga
     6541 ggaggcagcc tgctacacgc tcacgctggc cacggcctac cgtatgctct tcggccatcg
     6601 cccgcatgtg ctgagcgcag gccagaacgt gctggtctgg ggcgcctcgg gcggcctcgg
     6661 ctcgatggcg atccagctca tcgccacggt tggcgccaat gccatcggcg tgatctcgga
     6721 cgacgacaag accgacttcg tcaagcagct cggtgcgcgc ggcgtgatca accgcaagaa
     6781 cttcaactgc tggggacaga tgccccaggt cggcacgccc gcctacaatg aatggctcaa
     6841 gaacgcgcgc gagttcggca aggccatctg ggcctttacc ggcaagggca acaacgttga
     6901 catggtcgtc gagcatcccg gcgagtcgac cttcccggtc tccgtgctca tgacccgccg
     6961 cggcggcatg gtggtgatct gcgccggtac caccggcttc aatctcacca tggacgcgcg
     7021 ctacctgtgg atgcaccaga agcgcgtcca gggatcgcac ttcgcc
//
LOCUS       DBNGVR010000316         3867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4832779, whole genome shotgun sequence.
ACCESSION   DBNGVR010000316 DBNGVR010000000
VERSION     DBNGVR010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4832779"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="AC16A0_06705"
     CDS             complement(<1..610)
                     /locus_tag="AC16A0_06705"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3105059.1"
                     /translation="MADAAPPAAPKWADLGVRLISAVVLIPAVIADVWAGGIWFNLFV
                     ALLGVLMANEWVTICHRQDQRQFALHAAAAIAGALLPLEVGIWGSLAAIAVLWAISAG
                     FARYQDPAGAKWRYLGVPYVSLPAIALVLLRNDQVYGIAAIIWIMVTVWSADSLAYFA
                     GRIIGGPKLAPVISPKKTWAGLGGAVAGSALASLAFALIAGLP"
     gene            complement(610..1338)
                     /locus_tag="AC16A0_06710"
     CDS             complement(610..1338)
                     /locus_tag="AC16A0_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR3105060.1"
                     /translation="MPEMTKPVPRHVAIIMDGNGRWASQRGLPRAAGHRRGVEAVRRA
                     VRAAAELGIEYLTVYSFSAENWSRPQEEVSFLLDLLRRFIRTDVADLHKAGIRITVIG
                     DRTGLDDGLVKMIDEAEALTAGNSKLKLVVAFNYGSRQEITKVTRAIARKVAEGKIHP
                     DDITPAVIANHLDTGGMPDPDLLIRTGGEQRLSNYLLWQCAYTEFVFLPEYWPDFDAG
                     LLETAIAEFRGRDRRFGGLKVQSG"
     gene            complement(1367..1933)
                     /gene="frr"
                     /locus_tag="AC16A0_06715"
     CDS             complement(1367..1933)
                     /gene="frr"
                     /locus_tag="AC16A0_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3105061.1"
                     /translation="MAQPAPFNAQDLKRRMSGAVEALKHDFGGLRTGRASASLLEPIQ
                     VDAYGASMPLNQVGSVTVPEPRLLQISVWDRGLVIAVEKAIRASNLGLNPQTEGQVIR
                     LRMPDLTQDRRKELVKVAHQYAEKARVAVRNVRRDGMDQLKALEKEHLISEDEHKKKA
                     DDVQKMTDETIKEVDAALKHKEAEIMQV"
     gene            complement(1938..2657)
                     /gene="pyrH"
                     /locus_tag="AC16A0_06720"
     CDS             complement(1938..2657)
                     /gene="pyrH"
                     /locus_tag="AC16A0_06720"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3105062.1"
                     /translation="MARPSYKRILLKVSGEVLMGDQPFGIDLGTVGRVADEVMACAAA
                     GAQIALVIGGGNIFRGLAGAARGMDRVSADHMGIMATVMNALAMQGVLNAKGADARVL
                     SAIAMPSVCETYAPAKGRHHLEQGRIVICAAGTGLPFFTTDTGAALRAAELQCEALLK
                     GTSVDGVYSADPKTDPSARRFETITFSEILRHDLRIMDPAAIALARDNGIPVVVFSVR
                     KPGAIRDVLNGTGLFTTVTKD"
     gene            complement(2743..2955)
                     /locus_tag="AC16A0_06725"
     CDS             complement(2743..2955)
                     /locus_tag="AC16A0_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008039581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HIR3105063.1"
                     /translation="MKPDEIAKLTKFLRAKFELPSLAVRKRPQKNDSAEVYIGDEFIG
                     VIFRDEEDGELSYNFSMAILEIDLDE"
     gene            complement(2995..3819)
                     /gene="cysE"
                     /locus_tag="AC16A0_06730"
     CDS             complement(2995..3819)
                     /gene="cysE"
                     /locus_tag="AC16A0_06730"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR3105064.1"
                     /translation="MMRQAAKTVEKLDPVWDRIREEAESIARHEPALGGFIFSLVLNH
                     ESFEEALTHRLAQRLGNPDIGPDLIVHAFNDAIKAEPEIGHAARADIIATYDRDPACH
                     RYIDPLLYFKGYQSIQAHRMAHRLWDLGRKDFAYYLQSRSSMIASMDIHPAARIGKGI
                     MVDHGHDIVIGETAVIEDNVSIMQGVTLGGTGKESGDRHPKIRYGVLVGAGAKILGNI
                     EIGHCSRVAACSVVLHDVPANTTVAGVPAKVVGYAGCTEPARAMDHNVEPVAADPD"
BASE COUNT          714 a         1243 c         1204 g          706 t
ORIGIN      
        1 ccggcagccc ggcaatgagg gcaaaggcga gagacgccag ggcgctcccc gccacagcgc
       61 cgcccagccc ggcccaggtc ttcttcggcg agatcaccgg cgccagcttc gggccgccaa
      121 tgatgcggcc agcaaaatag gccagggaat ccgcgctcca gaccgtcacc atgatccaga
      181 tgatggcggc gatcccgtag acctggtcat tccgcaacag caccagggca attgccggca
      241 ggctgacgta gggtacgcca agatagcgcc attttgcgcc ggccggatcc tgatagcggg
      301 caaagccggc agaaatcgcc cagagcaccg caatggccgc gaggctgccc cagatcccca
      361 cttcgagcgg caagagcgcg cccgcgatgg cagcggccgc atgcaaggcg aactgacgtt
      421 gatcctgacg atggcagatc gtcacccatt cgtttgccat cagcacgcca agcaaggcaa
      481 cgaagaggtt gaaccagatg ccaccagccc agacatcggc gatgaccgcc ggaatcagca
      541 ccaccgccga aatcaggcga acgccgaggt cagcccactt cggagcggca ggcggcgcgg
      601 cgtcggccat cagccggact ggaccttgag cccgccaaag cgccggtcac gcccgcggaa
      661 ctccgcgatc gcagtctcaa gcaagcccgc atcgaaatcc ggccagtatt ccggaaggaa
      721 gacgaattcg gtgtaagcgc actgccaaag caagtagttc gacagccgct gttcgccgcc
      781 ggtccggatc agcaggtcgg gatcgggcat gccgcccgtg tcgaggtgat tggcaatcac
      841 ggcaggcgtg atgtcgtccg gatggatctt gccttcggcc actttacggg caatcgctcg
      901 cgtaaccttg gtgatttcct ggcggctgcc atagttgaag gcgaccacca gcttcagctt
      961 ggagtttccg gccgtcagcg cctcggcctc atcgatcatc ttgacgagtc catcgtccag
     1021 tcccgtccga tcaccgatga cggtgatgcg gatgcccgcc ttgtgcaggt cggccacatc
     1081 ggtccggata aagcggcgca gaagatcgag caggaaggac acctcctcct gcgggcgcga
     1141 ccagttctcg gcgctgaaac tgtagaccgt cagatattcg atgccgagct cggctgcggc
     1201 gcgaaccgca cggcggacag cctcgacgcc ccggcgatgc ccggccgccc gcggcaatcc
     1261 ccgctgcgaa gcccaccggc cattgccgtc catgatgatg gcaacatggc gcggcacagg
     1321 cttggtcatt tctggcaccg acgtcggcat tcggacctgt agcctctcac acctgcatga
     1381 tctcggcttc cttgtgcttc aatgcggcgt cgacctcctt gatggtctcg tcggtcattt
     1441 tctgcacatc gtcggccttc ttcttgtgtt cgtcctcgct gatcaggtgt tccttctcca
     1501 gcgccttgag ctgatccatg ccatcgcgcc gcacgttgcg caccgcgacc cgggccttct
     1561 ccgcatactg atgggcaacc tttaccagtt ccttgcgccg gtcctgggtg aggtccggca
     1621 tgcgcagccg gatgacctgc ccctcggtct gcggattgag gcccagattc gacgcccgga
     1681 tcgccttctc gacggcaatc accagtccac gatcccagac actgatctgc aggaggcgcg
     1741 gctcgggcac ggtgaccgag ccgacctggt tcaagggcat ggatgcgcca taggcgtcga
     1801 cttgaatcgg ttcgaggagc gaggcggagg cacgtccggt gcgcaggccg ccaaagtcat
     1861 gcttgagggc ctcgaccgcg ccgctcatgc ggcgcttgag atcctgggca ttgaagggcg
     1921 caggctgggc catgggttca gtccttggtc acggtggtaa agagccccgt gccattgagc
     1981 acatcgcgga tggcaccggg cttgcggacg gaaaacacaa ctaccggaat cccattgtcc
     2041 cgcgcaaggg cgatggctgc cgggtccatg atgcgcaggt cgtgccggag gatctcggaa
     2101 aaggtgatgg tttcgaagcg cctggcggac ggatcggtct tggggtcggc cgaatagacg
     2161 ccatcgacgc tcgttccctt cagcagggcc tcgcattgca attcggcggc acgcaaggcg
     2221 gctccggtat ccgttgtgaa aaacggcaat ccggtgccag cggcacagat cacgatgcgg
     2281 ccttgttcga gatgatggcg ccccttggcc ggcgcatagg tctcgcacac gctgggcatg
     2341 gcaatagcgg acaggacacg ggcatccgcg cccttggcat tgagcacgcc ctgcatggcc
     2401 agggcattca tcaccgtggc catgattccc atgtggtcgg ccgagacccg gtccatgccc
     2461 cttgccgcac cggcgaggcc ccggaagatg tttccgccgc cgatgacgag ggcgatctgg
     2521 gcacccgctg cggcacaggc catgacttca tccgccaccc ggccaacggt tccaagatca
     2581 atgccaaagg gctggtcgcc catcagcact tcgccggata ccttcaggag gattctcttg
     2641 tagctgggtc ttgccatggt gcgggtgccg atctggggcc tgcgcgcgat actagaaccg
     2701 ggaaacttga ttcgtcctca gggcttttgg aaggcagccg ccctattcgt cgagatcaat
     2761 ctcgagaatg gccatggaga aattgtagga caattcgcca tcctcctcat cgcggaagat
     2821 gaccccgatg aactcgtcgc cgatgtagac ctcagccgag tcgttcttct gcggccgctt
     2881 gcgcaccgcc aatgagggca gttcgaactt ggcgcgcagg aacttcgtga gcttggcgat
     2941 ttcgtcgggt ttcaaggcgg gcaactcctg aggggacctc gcgcccctct agcctcaatc
     3001 cggatcagcc gcaaccggct ccacattgtg gtccatggcg cgcgccggtt cggtgcagcc
     3061 ggcatagccg accaccttgg cgggaacgcc ggccaccgtc gtgttggcgg gaacgtcgtg
     3121 cagcaccacc gagcaggcgg ccacgcgcga gcaatggccg atttcgatgt tgccaaggat
     3181 cttcgcccct gccccaacca ggacgccgta acggatcttg ggatggcggt cgccgctttc
     3241 cttgccggtt ccgcccagcg ttacaccctg catgatcgag acattgtcct cgatcacggc
     3301 ggtttcgccg atcacgatgt catggccatg gtccaccatg atgcccttgc cgatgcgtgc
     3361 ggccgggtgg atgtccatcg atgcgatcat tgaggatcgg ctctgcaggt aataggcgaa
     3421 gtccttgcgc ccaaggtccc acaggcgatg ggccatccgg tgggcctgga tggactggta
     3481 acccttgaag tagagcagcg ggtcaatata gcggtggcag gccgggtcgc ggtcataggt
     3541 cgcgatgatg tcggcccgtg ccgcatggcc aatctccggc tcggccttga tggcatcatt
     3601 gaaggcatgg acgatcagat ccgggccaat gtccgggttg cccaggcgct gtgccagccg
     3661 atgcgtcagc gcttcctcga aactctcgtg gttcaggaca agcgagaaga tgaagccgcc
     3721 cagggcgggc tcatgccgtg caatcgactc cgcttcctcg cggatgcggt cccagaccgg
     3781 atcgagcttc tcgacggttt tcgctgcctg ccgcatcatg cactcccgtg ttcccacgaa
     3841 gtatacccct aaagtgccga catgggg
//
LOCUS       DBNGVR010000317         2846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_314697, whole genome shotgun sequence.
ACCESSION   DBNGVR010000317 DBNGVR010000000
VERSION     DBNGVR010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_314697"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="AC16A0_06735"
     CDS             <1..1831
                     /locus_tag="AC16A0_06735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3105065.1"
                     /translation="HYWFAAHGYGVARVDISGSGDSDGLIEDEYHKREQDDALEIIAW
                     LAAQEWCTGKVGMIGISWGGFNGLQVAARRPPALKAVVSLCSTVDRYADDVHFMGGCL
                     LNDTLDWGCYFVACGSLPPDPEIVGKNRWKEMWAYRLENLDCYPMLWMEHQRRDAFWK
                     HGSVCEDYGKIEIPVLNVSGWVDGYTAAVFRSVENMPGAKGIAGPWGLKYPHVGIPGP
                     AIGFLQECDRWFGRWLKGKKTGVEDDPPIRVFLQDGETPRPHFDARKGRWLGLPLWPS
                     PNIRTEMLYLGDRKLLAAKTRVKPLSVCSSFTTGVTSGEWCAYGLGKVAPELAIDQRE
                     DDAQSLCFDGPALKKPISIIGRIPVRLRVAANKPQAQLAVRLNAIHPDGTVERISYGL
                     LNLSHRDSHETPKPLKPGQFYGVTVELNEIAQTVPAGAALRLAISTSYWPIAWPSPEL
                     ATVTVDPARSSIELPVLKSEKGLRKVAFKPAVKSAPAPVTVKDSGAETRHMILDIERQ
                     RMNFTIRRNDGSYVIDEIGTQISLTKLKDFAVSRDGKDPPRSLVATTVHYQRGDWDAR
                     TEAEVSMSSDKTHFHMESHVRCFVDGKPFMNRSFKRSIKRDGV"
     gene            2018..>2846
                     /locus_tag="AC16A0_06740"
     CDS             2018..>2846
                     /locus_tag="AC16A0_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105066.1"
                     /translation="MMNRRDLLKRSSFLAAIGMLGIRPEQVLAQEGDILKVRMEADIQ
                     VLDPGYMIGGAETTIQFACLPRLAVPTKQADGKWGWVPSEYVEKISQDDPVTISFTLK
                     PGLMWTKDGGEVTAEDVKFSFERMLKSDWAARFPTLERVDVKDKLNGVIVLKAPFAAT
                     FLMGLASESGTIVPKAIVEKLPDQKFTTELPAQCGPYTMVEWTPKQRIVLKSNPDWKG
                     SKPDFPEVHLINVEDTSAAELAFEAGEVMITNVLPATAARYRDKMPEGATVENLAGPY
                     "
BASE COUNT          564 a          881 c          891 g          510 t
ORIGIN      
        1 ccactactgg ttcgccgccc atggctatgg cgtggcccgc gtcgacattt cgggctcggg
       61 cgacagcgac gggctgatcg aggacgagta tcacaagcgc gaacaggatg acgcccttga
      121 gatcatcgcc tggctggctg cgcaggagtg gtgcaccggg aaagtcggca tgatcggtat
      181 ttcctggggc ggattcaacg gcctccaagt tgcggcaaga cggccgccgg cgctgaaggc
      241 cgtcgtgtcg ctctgttcga ccgtcgaccg ctatgccgat gacgtgcact tcatgggcgg
      301 ctgcctgttg aacgatacgc tcgattgggg ctgctacttt gtcgcctgcg gatctctgcc
      361 gcccgatccg gaaatcgtcg gcaagaatcg ctggaaggag atgtgggcct accggctcga
      421 gaatctcgac tgctatccca tgctgtggat ggagcatcag cgccgcgacg ccttctggaa
      481 acatggctca gtctgcgagg actacggcaa gatcgagatt ccggtgctca atgtgagcgg
      541 ctgggttgac ggatatacgg ccgcggtatt ccgttcggtc gagaacatgc cgggcgcgaa
      601 gggtattgcc ggaccctggg gactcaagta tccgcatgtc ggcattccgg ggcccgccat
      661 cggattcctg caggaatgcg accggtggtt cggccgctgg ctgaagggca agaagaccgg
      721 cgtggaggat gatccgccga tccgcgtctt cctgcaggat ggcgaaacgc caagaccgca
      781 cttcgatgcg cgcaaggggc gctggctggg cctgccccta tggccgtcgc ccaacatccg
      841 gaccgagatg ctttacctcg gtgatcgcaa gctgctcgca gcgaaaaccc gcgtcaagcc
      901 gctgtcggtc tgttccagct tcaccacggg ggtaacttcc ggagagtggt gcgcctacgg
      961 tcttggcaag gtcgcgcccg aattggccat cgaccagcgc gaggacgacg cgcaatctct
     1021 gtgtttcgac ggcccggcgc tgaagaagcc gatctccatc atcgggcgca ttcccgtgcg
     1081 gctgcgcgtg gcggccaaca agccgcaggc ccagctggcg gtcaggctca atgcgatcca
     1141 tcccgatggc accgtcgagc gcatcagcta cggacttctg aacctgtcgc accgcgacag
     1201 ccacgaaacg cccaagccgc tgaagcccgg ccagttctat ggcgtgacgg tggaactgaa
     1261 cgagattgca cagaccgtgc cggcgggcgc agcccttcgt ctggccatat caaccagcta
     1321 ctggcccatc gcctggccgt cgccggagct ggcgacggta actgtcgatc ccgcaagatc
     1381 ctcgatcgaa ctcccggtcc tgaagtccga aaagggcttg cgcaaggttg cgttcaagcc
     1441 ggccgtcaag tcggcgccgg cgccggtcac cgtcaaggac agcggcgcgg agacccgcca
     1501 catgatcctc gacatcgaaa ggcagcggat gaacttcacc atcaggcgca acgacggcag
     1561 ctatgtcatc gacgaaatcg gtacgcagat cagccttacc aagctgaagg acttcgcagt
     1621 cagccgcgac ggcaaggatc cgccccgctc gctggtcgca acgacggtgc actaccagcg
     1681 cggagactgg gatgcgcgca ccgaagccga ggtttcgatg tcatcggaca agacgcactt
     1741 tcacatggaa agccatgtgc gctgctttgt cgacggcaag cccttcatga atcgcagctt
     1801 caagcggtcg atcaagcggg atggcgtgtg aagccttggc tttccgtaaa ggcagggcgc
     1861 cgtatccagc ctgcttctgc ctgcaatttg ggcgcctctc aggcaaacgg gaggcacgga
     1921 tcggtttgac atcgaccagt gcccggtcca gtctttgatg tccaggattt caaagggctc
     1981 acttaccaat agacaacaca gcttcaggga gacgtccatg atgaaccgcc gtgacttgct
     2041 caagcgctct tctttcctcg ccgcgatcgg catgctcggc atccggccag agcaggttct
     2101 ggcccaggag ggcgacatcc tcaaggtccg catggaagcc gacatccagg tcctcgatcc
     2161 cggctacatg atcggcggcg cggaaacgac aatccagttc gcctgcctgc cccgcctggc
     2221 tgttccaacc aagcaggccg atggcaagtg gggctgggtg ccgtccgaat atgtcgagaa
     2281 gatctcgcag gacgatccgg tcaccatctc cttcacgctg aagcccggcc tgatgtggac
     2341 caaagatggc ggcgaggtga cggcagagga cgtgaagttc tcctttgagc gcatgctgaa
     2401 gtccgattgg gccgcgcgct tccccacgct cgagcgggtc gacgtgaagg acaagctcaa
     2461 tggcgtgatc gtgttgaagg cgcctttcgc tgccacattc ctgatgggct tagcctccga
     2521 gtccggcacg atcgtgccca aggccattgt cgagaagctg cccgatcaga agttcaccac
     2581 cgaactgccc gcgcagtgcg gcccctacac catggtggag tggacgccga agcagcgcat
     2641 cgttctgaaa tccaatcccg actggaaggg cagcaagccc gatttcccgg aagtccattt
     2701 gatcaacgtg gaagacacca gcgccgccga actcgcattc gaagccggcg aggtgatgat
     2761 caccaacgtg ctgccggcga cggcggcacg ctatcgcgac aagatgccgg agggcgccac
     2821 ggtcgaaaac ctggcggggc cctatt
//
LOCUS       DBNGVR010000318         2537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_314750, whole genome shotgun sequence.
ACCESSION   DBNGVR010000318 DBNGVR010000000
VERSION     DBNGVR010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_314750"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16A0_06745"
     CDS             complement(<1..235)
                     /locus_tag="AC16A0_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105067.1"
                     /translation="MRKIFTPLTLYAILFTIFLYGPVLLMPLFSFNDNTFAVFPLKGF
                     TLKHYASMAANGSMLGALWNSLAVATIVAVVATA"
     gene            complement(237..1166)
                     /locus_tag="AC16A0_06750"
     CDS             complement(237..1166)
                     /locus_tag="AC16A0_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105068.1"
                     /translation="MPRQTAAGLSGPAAGYALLSPAMLIIVAMLVAPMLALVTLSFWT
                     QNGFELDTSFTLANYWALIELADEPTMWMGIPFPFENPVYAILMLKSLVMSLAVTAAV
                     ILIAYPMAYFLAFRVTRYKALWIIIITIPFWTSYLLRVFSWKIMLGFNGVINSGLMSL
                     GIISEPLSFLLYNPPAVVITLTHAWVTFAVLPIYVSLEKIDRSLLEAATDLGDSKWQR
                     FRRVTLPLSLPGTISAALLVFIPTVGDYVTPTLVGGPGGTMIGNSIQSLFLKQDNSPR
                     GAALSIVMMAIVALLSLLFMWAVGYRKIRARSA"
     gene            complement(1186..2304)
                     /locus_tag="AC16A0_06755"
     CDS             complement(1186..2304)
                     /locus_tag="AC16A0_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105069.1"
                     /translation="MHRIKFIDRMSTGKLSRREMLKSATAFGVGLTVLPRLGAAAEVL
                     TCLEWAGYDAPDYFGSFVNKHGGPPNFSIFAGEEDALAKVRAGFAADVMHPCNYSVNR
                     FVSAGLTNPIDTAKLSNWKDIFPALQTADGVVSGGNVVMAPADWGNSSIAYRPDLIGD
                     AFKDGETWAIFYDEKYKDKVALLDDPLVIQIGAMVNGMPYDQAYKLAGADLEAAKAMA
                     AKAVQINRMLWTDATEIQQGLASGEIIAAYSWNDTIKNLMGQGIPVAYAKPKEGYFTW
                     FCGLTLLNSGKADPALAYDFIDAWLSPETGKALIDGSGYGHANLKSFEVAQADSIVAM
                     GIKDPVEHMKSAILFKTQENEVTNEQNKVWEELKALKQ"
BASE COUNT          527 a          728 c          837 g          445 t
ORIGIN      
        1 cggcggtcgc aaccaccgcg acgatggtcg cgacggcgag cgagttccac agggcaccga
       61 gcatcgaacc attggcggcc atcgatgcat aatgcttgag cgtgaagccc ttcagcggaa
      121 acacggcaaa cgtgttgtcg ttgaaggaga agagcggcat cagaagcacg gggccataga
      181 ggaagatcgt gaaaaggatg gcgtagagcg tgagaggggt gaagatcttc ctcatctcag
      241 gcactccggg cccggatctt gcgatatcct accgcccaca tgaagaggag cgagagcagg
      301 gccacgatcg ccatcatgac gatggagagt gcggcaccac gcggcgagtt gtcctgcttg
      361 agaaagagcg attggatcga gttgccgatc atggtgccgc ccggtccgcc aaccagcgtg
      421 ggcgtcacgt agtcaccgac ggtcggaatg aagaccagca gggcggccga gattgtgccc
      481 ggcagcgaga gcggcaaggt cacgcgccgg aagcgctgcc acttgctgtc acccaagtcg
      541 gtggcggcct caagcagcga acggtcgatt ttctcgagcg agacatagat gggcagcacc
      601 gcgaatgtga cccaggcatg ggtgagcgtg atcacgacgg ccggcggatt gtagagcaga
      661 aagctcaggg gctccgagat gataccgagg ctcatcaggc cggagttgat gacgccgttg
      721 aagcccagca tgatcttcca ggaaaagacg cgcagcagat agctggtcca gaagggaatc
      781 gtgatgatga tgatccacag cgccttgtag cgcgtcaccc gaaaggccag gaaataggcc
      841 atgggatagg cgatcaggat gacggctgcc gtcaccgcca gcgacatcac cagcgatttc
      901 agcatgagga tcgcgtagac gggattctcg aaggggaagg gaatccccat ccacatggtc
      961 ggttcgtccg ccagctcgat caatgcccag tagttggcca gcgtgaaact ggtgtcgagc
     1021 tcgaagccgt tctgcgtcca gaagctgagc gtcaccaagg ccagcatcgg cgcgaccagc
     1081 atggcaacga tgatcagcat ggccggcgag agaagcgcat agcccgccgc cgggcccgaa
     1141 agcccggcgg cggtctgtcg cggcattgcg tccggcacgg cggcgctact gcttgagggc
     1201 cttgagctct tcccagacct tgttctgttc gtttgtcact tcgttctcct gggtcttgaa
     1261 caggatcgcg ctcttcatgt gctcgaccgg atccttgatg cccatggcga caatggaatc
     1321 ggcctgcgcg acctcgaagg acttcaggtt ggcatggccg tagccggagc catcgatcag
     1381 ggccttgcca gtctcgggcg acagccaggc atcgatgaag tcataggcaa gtgccggatc
     1441 ggccttgccg gaattgagca gcgtcaggcc gcagaaccag gtgaagtaac cttccttcgg
     1501 cttggcatag gcgaccggaa tgccctgacc catcaggttc ttgatggtgt cgttccagga
     1561 ataggccgcg atgatttcgc cggacgcgag accctgctgg atctcggtgg cgtcggtcca
     1621 cagcatgcgg ttgatctgaa ccgccttggc ggccatggcc ttggccgctt cgagatcggc
     1681 gccggccagc ttgtaggcct gatcataggg catgccgttg accatggcac cgatctggat
     1741 caccagagga tcgtcgagca gcgcgacctt gtccttgtac ttttcatcat agaagatggc
     1801 ccaggtctcg ccgtccttga aggcatcgcc gatcaggtcg ggacgatagg cgatggagga
     1861 gttgccccag tcggcgggcg ccatgacgac attgccgccg ctgaccacgc catccgctgt
     1921 ctgcagggcg ggaaagatgt ccttccagtt ggagagcttc gcggtatcga tgggattggt
     1981 cagtccggca gagacgaaac ggtttaccga gtaattgcag ggatgcatga cgtcggcggc
     2041 gaagccggcg cggaccttgg cgagggcatc ttcctcgccc gcgaaaatcg agaagttcgg
     2101 cggaccgcca tgcttgttga cgaaggagcc gaaatagtcc ggcgcgtcat agccggccca
     2161 ttcgaggcag gtcagaactt cggccgcagc gccgagacgc ggcagcacgg tcaggccgac
     2221 gccgaaggcg gtggcgctct tcagcatctc gcggcgggac agcttcccgg tcgacatgcg
     2281 gtcgataaac ttgatgcgat gcattagggg cttcctccgt tgtgttctgg ttattgttgc
     2341 aaacaataaa ccggaagccg cggaaaacgg aagcccttct ctcgaacctc agcgccggcc
     2401 ggcgccggaa acgcggctca tctcctcgct ggcatgggcc gccagcacgc gtcccgcaag
     2461 gtaaagcgag ccgcagatga gaatgcgggg cgcaggtgcc gccagcgccg cctggcgcag
     2521 ggcctcgtcg agcgagc
//
LOCUS       DBNGVR010000319         3496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_709119, whole genome shotgun sequence.
ACCESSION   DBNGVR010000319 DBNGVR010000000
VERSION     DBNGVR010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_709119"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="AC16A0_06760"
     CDS             complement(<1..1585)
                     /locus_tag="AC16A0_06760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HIR3105070.1"
                     /translation="MDYIQPYLDFFAAHPGWALAIVFLIAFGEALLIIGLFVPSTAVL
                     VGAGILVGTGHLAFWPVLAATTIGAILGDQVSFWAGRFFGESLKTMWPLSRYPQLVAR
                     GEDFVRQHGGKSIAIGRFVPGVKSVVPGIVGMFGMKELYFLVVNVSSAIVWGAAHILP
                     GVLIGQGLAFAGELSGRLLFVLLVLLVLLAVGGYIIRLAAAGLTPFLNRTLSRVSAWA
                     AGFDNRSMQRFARAAAPENPRSALLVLFAAIIITGLIAIVNMIVHFASGDAVSNMDVS
                     VFNLMQEMRNTPADELMIAITMMGDGLVISALAIAIVAWLVWHRSYRAALAAAAVILA
                     GKIFVPIMKTLIQRARPIELYQGAEGYSFPSGHATMAALTFGVLAVLVSHSMGRWGRA
                     VVYASCGLVVIAIAYSRVYLGVHWLSDVLGGMLFGAVMAAVYGVVIEAVPPRRIRALG
                     LMGASFIIFLLAGAFHVSTGFVRAEQFYGPSRQTIVLPATEWEGAGWRRLPDHRIDLG
                     GGRRPEPFVAQYAGDIERLA"
     gene            complement(1654..1887)
                     /locus_tag="AC16A0_06765"
     CDS             complement(1654..1887)
                     /locus_tag="AC16A0_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105071.1"
                     /translation="MRNVLIIAMLLVARPAVAMNWEGHDDAFADDPYAKAFMQAAPHA
                     KPLPSRACPVSEEQASANAYEQIPLPRHGCPEN"
     gene            2472..>3496
                     /locus_tag="AC16A0_06770"
     CDS             2472..>3496
                     /locus_tag="AC16A0_06770"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126694.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIR3105072.1"
                     /translation="MRIDRRFTKQGQDSYAGLEFRKAVSEIKNPDGSVVFRQENIEVP
                     ASWSQVATDILAQKYFRKAGVPAILKRVEENSVPSWLWRSEADEEALAVLPEKERIIG
                     ERSSKDVFNRLAGTWTYWGWKGGYFDSEEDARAFYDEMCFMLATQKCAPNSPQWFNTG
                     LHWAYGIDGPGQGHYYVDFETGRLTKSKSAYEHPQPHACFIQSVADDLVNEGGIMDLW
                     VREARLFKYGSGTGSNFSELRGENEKLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVIVDVDHPDIEDFIEWKVKEEQKVAALVTGSKTVKKHLQAVMKACVNCEGPGDD
                     CYEIEKNP"
BASE COUNT          744 a         1064 c         1131 g          557 t
ORIGIN      
        1 ctgcgaggcg ttcgatgtcg cccgcatatt gggccacgaa gggttcgggc ctgcggccgc
       61 cacccagatc gatgcgatga tcgggaagcc ggcgccaccc ggcgccctcc cattccgttg
      121 ccgggagaac gatggtctgg cgggacggtc catagaactg ttcggcccgg acaaaaccag
      181 tggagacatg gaaggcaccg gccagcagga agatgatgaa ggaggcaccc atgagcccaa
      241 gcgcccggat gcggcggggc ggcacggcct cgatgacgac gccgtagact gcggccatga
      301 cggcgccgaa gagcatgccc ccgaggacat ccgacagcca gtggacccca agatagacgc
      361 gcgaataggc aatggcgatc accaccagtc cgcagctggc gtagacgacc gccctgcccc
      421 agcgccccat ggaatgactg accagtaccg ccagcacgcc gaaggtgagt gcggccatgg
      481 tggcatggcc tgacgggaag ctgtaaccct cggcgccctg gtagagctcg atcggcctgg
      541 cccgctggat gagggtcttc atgatgggca cgaagatctt gccggcgagg atgacggccg
      601 ccgccgccag cgccgcgcgg taggagcggt gccagaccag ccacgccacg atggcgatgg
      661 ccagtgcgct gatcaccagc ccgtcgccca tcatggtgat ggcgatcatg agttcgtcgg
      721 ccggcgtgtt gcgcatctcc tgcatgaggt tgaagaccga cacgtccatg ttggacacgg
      781 cgtcgcccga cgcgaagtgg acgatcatgt tcacaatcgc gatgagcccg gtgatgatga
      841 tggccgcgaa cagcaccagc agcgccgaac gcggattctc gggcgcagcc gcgcgcgcga
      901 aacgctgcat cgaacggttg tcgaaaccgg cggcccaggc cgaaacgcga gagagcgtgc
      961 ggttgagaaa gggtgtaagg ccggcggcgg ccagccggat gatgtagccc cccacggcca
     1021 gcaggacgag cagcacgagc agcacgaaga gcagccgtcc gctcagttcg ccagcaaagg
     1081 cgaggccttg cccgatcaac acgcccggca ggatgtgagc ggcgccccag acaatggccg
     1141 aggaaacatt cacgacgaga aaatagagtt ccttcatgcc gaacatgccg acgatgccgg
     1201 gcaccaccga cttcacgccg ggcacgaagc ggccgatggc gatgctcttg ccgccatgct
     1261 ggcgcacgaa gtcttcgccg cgcgccacca gctgcggata gcggctcagc ggccacatgg
     1321 tcttcagact ctcgccgaag aaccggccgg cccagaagga cacctggtcg ccaagaatcg
     1381 cgccgattgt cgtggcggcc agcaccggcc agaacgcgag atgtccggtc cctaccagaa
     1441 tgccggcacc cacaagtacg gcagtcgacg gcacgaagag cccgatgatc agcagggctt
     1501 cgccgaaggc aatcaggaat acgatggcaa gggcccatcc gggatgggcc gcgaagaaat
     1561 ccagataggg ctgaatatag tccatgcggg ttggagcctt aacccagtca cggttttccg
     1621 caaagcggct tgatcatggc gcggggccgc cggtcagttc tccgggcagc catgccgcgg
     1681 gagcggaatc tgttcgtaag cattggccga cgcctgttct tcgctcaccg ggcaagcgcg
     1741 actcgggagc ggtttggcgt ggggagctgc ctgcatgaag gccttggcat agggatcgtc
     1801 ggcgaaggca tcgtcatggc cttcccagtt catggccacc gccggcctgg cgacgagcag
     1861 catcgcgatg atcaggacgt ttcgcatgat tctgaaccta acagcatgtt ccggccccgg
     1921 cgactcacgt tctcctgaac gtccgatgca atcagagcga aattccattc gacacacacc
     1981 ctcatggcgg gaaacgcgat cctatcaaca ggcatcttcc gcgcctggtc ttaacgggcc
     2041 ctttaccatg gtctaagacc gccaccagat tcgcggcggc ctatcggatt cgcgtcccaa
     2101 ggcaagccct gatcgcccgg atcgagggtt ttcgcctaag gccacgaaac tcttgagctt
     2161 catccgagaa tcggatcgac tatgggttat ccacatcctg cgtcaagctg tggacacaag
     2221 atattgtggt aagctttcgt taacacacta gccattgacg acgtgacccc gcctgttcta
     2281 tcttttgctc aaggctgcgg ggtggagaca agagccaaaa ggctcctcca agttggatac
     2341 ttcacaagaa gagccgatcg accggggacg gctattcccg gacaggtgtg tgtcgttgaa
     2401 cgcaggtgga gaggcctcgc gggcgttgaa tcacgcgtcc gcgagtaaca attggaagag
     2461 ggaagaagaa gatgcgcatc gatcgccggt tcaccaagca gggccaggac agctacgcgg
     2521 gactcgagtt ccgcaaagcc gtcagcgaga tcaagaatcc ggacgggtct gtggtgttcc
     2581 gccaggagaa catcgaggtg ccggcctcgt ggagccaggt cgcgaccgat atcctcgcgc
     2641 agaagtattt ccgcaaggcg ggcgtcccgg cgattctgaa gcgcgtggaa gagaacagcg
     2701 ttccctcctg gctgtggcgt tcggaagcgg atgaggaagc gcttgcggta ctgcccgaga
     2761 aggaacgaat catcggcgag cgctcgtcca aggacgtgtt caaccgtctt gccggcacat
     2821 ggacctattg gggctggaag ggcggctatt tcgacagcga ggaagatgca cgcgccttct
     2881 atgacgagat gtgcttcatg ctggcgacac agaaatgcgc gccgaactcg cctcagtggt
     2941 tcaacaccgg cctgcactgg gcctatggca tcgatgggcc cggccagggc cactactacg
     3001 tcgacttcga gacgggccgc ctgaccaagt cgaagtccgc ctatgagcac ccgcagcccc
     3061 atgcctgctt catccagtcg gtggccgacg acctcgtcaa cgagggcggc atcatggacc
     3121 tgtgggtgcg cgaggcgcgc ctgttcaaat atggctccgg caccggttcc aacttctccg
     3181 aactgcgcgg cgagaacgag aagctctcgg gcggcggcaa gtcgtccggc ctcatgagct
     3241 tcctcaagat cggcgaccgg gcggcgggcg ccatcaagtc cggcggcacc acgcgtcgcg
     3301 cggccaagat ggtgatcgtc gacgtcgacc atccggatat cgaggacttc atcgagtgga
     3361 aggtgaagga agagcagaag gtggcggccc tcgtcaccgg ctccaagacg gtgaagaagc
     3421 atctgcaggc cgtgatgaag gcctgcgtga actgcgaagg cccgggagac gactgctacg
     3481 agatcgagaa gaaccc
//
LOCUS       DBNGVR010000320         4104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2477111, whole genome shotgun sequence.
ACCESSION   DBNGVR010000320 DBNGVR010000000
VERSION     DBNGVR010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2477111"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16A0_06775"
     CDS             complement(<1..202)
                     /locus_tag="AC16A0_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105073.1"
                     /translation="MAILFVLLLVALVALIYVFLDRREKRRAAAGEARLSALRLIFGA
                     AAALLMLFAGGCSLLFLINQDGA"
     gene            complement(204..434)
                     /locus_tag="AC16A0_06780"
     CDS             complement(204..434)
                     /locus_tag="AC16A0_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105074.1"
                     /translation="MHVLAVIAGLLLLIFAGGCTIWSVSDGHFSTLEQVPWGLMFVAI
                     FGALPAFAGWKLFRFGMSFSRNDAHSKPDGEA"
     gene            complement(436..681)
                     /locus_tag="AC16A0_06785"
     CDS             complement(436..681)
                     /locus_tag="AC16A0_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105075.1"
                     /translation="MHVLAIIFGLLLLGFGGGCTVLIVGGGLLSDPAALIADIPLLTT
                     ILVPFGILPAIGGWFLFRWGLKTDRARRRAPDKTGEA"
     gene            complement(683..1045)
                     /locus_tag="AC16A0_06790"
     CDS             complement(683..1045)
                     /locus_tag="AC16A0_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105076.1"
                     /translation="MKVLLSILCGLIALFAGGCTLLAMSGAGLSGVLASGPLILIPAA
                     VAILNVMVLAALWGFARPNRGILVTLLVLDVLVVLALAATWASLGGGDRDMNILAGLL
                     IGGFAAKGLLTGLYLRSV"
     gene            complement(1042..2454)
                     /locus_tag="AC16A0_06795"
     CDS             complement(1042..2454)
                     /locus_tag="AC16A0_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3105077.1"
                     /translation="MNALIRKVAPYVFYGQTTSMCDDCMALVPAKIIIEDSHVYYLKR
                     CRTHGVQKALVSTDAAYYKLCHDYLKPGDRPLAPQTRTEQGCPYDCGLCPDHEQHSCL
                     ALIDVNEACNLTCPVCFADSSVQKTAHRPLAEIERMMDALVASEGEPDLLQLSGGEPT
                     IHPQIIEIIAAAKQRPIRHIMLNTNGIRIAQDPDFVKRLAAFKPGFEVYLQFDSLRAS
                     VHKNLRGADLTRIRRDALEALERESISTTLVVVVKRGQNDDELGEIIDFALQWKCVRG
                     ITFQPIQDAGRNEGFDPARDRFVLSDIRRRIIAAKTPFGAGDIIPLPCNPESIAIGYA
                     LRSGSKIAPITSFFPKDMLVEALPNAITFEKYPDLHKQILNFFSLSTAECNNPEKLDA
                     LLCCLPQIPVPEGMGYENIFRIAIVEFMDRHNFCISRVKRSCIHFVAPSGQIIPFETY
                     NMFYRDAAARRRMAVSMGQG"
     gene            complement(2451..2891)
                     /locus_tag="AC16A0_06800"
     CDS             complement(2451..2891)
                     /locus_tag="AC16A0_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105078.1"
                     /translation="MTRADFWCRVVGWLQIAGGATLAFTLLFLWELALDLVGIESVPG
                     LSFLVWVFVLMMALPMFLAGLFTVLFANAVEQSREGLRGQSKAFLRIVMALSGLWAAG
                     IVGFAGMTVPPVGVFTVLALATTIIAIMGPDWTADLFGRKESTA"
     gene            complement(2888..3580)
                     /locus_tag="AC16A0_06805"
     CDS             complement(2888..3580)
                     /locus_tag="AC16A0_06805"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3105079.1"
                     /translation="MILHTVFDLAAAVAAMTVTALVYRWRLTDAGQKIEHAGVGYAAA
                     LLAGAAAGGFGAGTLNLWLSGAPGIGRSIVGALAGAILAIELFKRWRGIAGSTGLIFV
                     PAFATSVVVGRWGCFLTGIEDNTHGTPTSLPWGHDFGDGLLRHPVQLYESFTMLAFLV
                     VALVLIGRRNPFFMRNGFYLLVLVYAGQRFVWEFLKSYGAVIGPFNLFHLICAGLVIY
                     AVIMMRGQGKQT"
     gene            complement(3577..3879)
                     /locus_tag="AC16A0_06810"
     CDS             complement(3577..3879)
                     /locus_tag="AC16A0_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105080.1"
                     /translation="MAISGDHGQHPTHAHIMLAGWVSMALMGLFYHHFPALNLSVMAR
                     VQFWVTALSAVLMCVSLIFLYGGNPAVEPGAAIGSIGFLLGMLLFAYNALGTIWRS"
BASE COUNT          837 a         1255 c         1392 g          620 t
ORIGIN      
        1 aggcgccatc ctggttgatc aggaaaagca gcgagcagcc gcctgcgaac agcatcagca
       61 gggccgcggc cgcaccgaag atcaggcgca gggccgataa ccgcgcctcg cccgcggcag
      121 cgcgccgttt ctccctgcgg tcgaggaaga cgtagatcag ggccacgagg gcaaccagca
      181 ggaggacgaa gagtattgcc atgtcaggct tcaccgtccg gcttcgagtg agcatcattc
      241 cggctgaagc tcatgccgaa tctgaacagc ttccagccgg cgaaggccgg cagtgccccg
      301 aagatggcaa cgaacatcag cccccagggc acctgctcga gagtcgaaaa atggccgtcg
      361 gagaccgacc agatcgtaca gccgccggcg aagatcagca gcagcaggcc ggcgatcacg
      421 gcgagcacat gcatgtcagg cctcccccgt cttgtcaggc gccctgcgcc tggcgcggtc
      481 cgtcttcagg ccccagcgga acaggaacca gccgccgatg gcaggcagga tgccgaaggg
      541 cacgaggatg gtggtgagga gcgggatgtc ggcgatcagc gcggccgggt cagacagcaa
      601 tccgccaccc acgatcagaa cggtgcagcc cccgccaaag ccgagcagga ggagaccgaa
      661 gatgatcgcg aggacatgca tgtcacaccg accgcagata gaggccggtg agcaggccct
      721 tggccgcgaa gccgccgatc agcaggcccg cgaggatgtt catgtccctg tcgccgccgc
      781 ccagcgacgc ccatgtggcc gcgagcgcaa ggaccacgag gacgtcgagc accagcaagg
      841 tgacgaggat gccgcggttc ggcctggcga aaccccagag cgcggccagc accatgacgt
      901 tgaggatcgc gacggcggcg gggatcagga tcagcgggcc cgatgccagc acacccgaga
      961 gcccggcgcc gctcatggcc agcagcgtgc agccgccggc gaacagcgcg atcagcccgc
     1021 agagtatgct gagcaggacc ttcatccctg ccccatcgac acggccatgc gccggcgcgc
     1081 cgcggcatcg cggtagaaca tgttgtaggt ctcgaaggga atgatctggc cgctgggcgc
     1141 cacgaaatgg atgcaggagc gcttcacccg gctgatgcag aagttgtggc ggtccatgaa
     1201 ctcgacaatg gcgatgcgga agatgttctc gtagcccatg ccctctggca ccgggatctg
     1261 cggcaggcaa cagagcagcg catcgagctt ttcgggattg ttgcattcgg cggtcgagag
     1321 cgagaagaag ttcaggatct gcttgtgcag gtccgggtac ttctcgaagg tgatcgcgtt
     1381 gggcagcgcc tcgaccagca tgtccttggg aaagaaggac gtgataggcg cgatcttgct
     1441 gccgctgcgc agcgcatagc cgatggcgat cgattccgga ttgcagggca gcggaatgat
     1501 gtcgccggcg ccgaagggcg tcttcgccgc gatgatccgg cggcggatgt cggagagcac
     1561 gaaacggtcg cgcgccgggt cgaagccctc gttgcggccg gcgtcctgga tgggctggaa
     1621 cgtgatgccg cgcacgcatt tccattgcag ggcgaaatcg atgatctcgc ccagctcgtc
     1681 gtcgttctgg ccgcgcttca cgaccacgac gagggtggtg gaaatcgatt ctctttcaag
     1741 ggcttccagc gcgtccctgc gaatccgggt gaggtcggca ccgcgcagat tcttgtggac
     1801 ggaggcccgc agcgaatcga actggagata gacctcgaag cccggcttga aggccgccag
     1861 ccgcttcacg aaatccgggt cctgggcaat gcggatgccg ttggtgttga gcatgatgtg
     1921 gcggatgggc cgctgcttgg ccgccgcgat gatctcgatg atctgcgggt ggatggtggg
     1981 ttcgccgcct gacagttgca ggaggtcggg ttcgccttcg ctggcgacca gcgcatccat
     2041 catgcgctcg atctcggcca gcgggcgatg cgccgtcttc tgcaccgaac tgtcggcaaa
     2101 gcagacgggg catgtgaggt tgcaggcctc gttgacgtcg atgagcgcca ggcaggaatg
     2161 ctgttcgtga tccgggcaga ggccgcagtc ataggggcag ccctgttcgg tgcgggtctg
     2221 aggcgcgagc gggcgatcgc ccggtttcag atagtcgtgg cagagcttgt agtaggccgc
     2281 gtcggtggag acgagcgcct tctgaacgcc atgcgtccgg cagcgcttga ggtaatagac
     2341 atgcgagtcc tcgatgatga tcttggccgg caccagggcc atgcagtcgt cgcacatgga
     2401 cgtggtctgg ccgtagaaga catagggagc gaccttgcgg atgagtgcgt tcatgcggtc
     2461 gactccttcc gcccgaagag atctgccgtc cagtcgggac ccatgatggc aatgatcgtg
     2521 gtggcgaggg ccagcaccgt gaagacgccg accggcggta ccgtcatgcc ggcaaagccc
     2581 acgatgccgg cggcccaaag ccccgacagc gccatgacga tccgcaggaa ggccttggac
     2641 tgcccacgca ggccttcgcg cgattgctcc acggcattgg cgaagagcac ggtgaacagg
     2701 ccggcaagga acatgggcag cgccatcatc aggacgaaga cccagacgag aaacgacagg
     2761 cccggaacgc tctcgatgcc gacgaggtcg agggccagtt cccacaggaa cagaagggtg
     2821 aaggccaggg tcgcgccacc tgcaatctgc agccagccca ccacccggca ccagaaatcg
     2881 gcgcgcgtca tgtctgcttc ccctgccccc gcatcatgat gacagcgtag atcacgagcc
     2941 cggcgcaaat caaatggaac aggttgaagg ggccgatgac ggcgccatag gacttgagaa
     3001 actcccagac gaagcgctgc ccggcataga ccagcaccag gagatagaag ccgttgcgca
     3061 tgaagaaggg attgcggcgg ccgatcagca ccagtgccac cacgaggaag gccagcatgg
     3121 tgaaggactc gtagagctgc accgggtggc gcaggagacc gtcgccgaag tcgtgtcccc
     3181 agggcaggct ggtgggcgtg ccatgcgtgt tgtcctcgat accggtgagg aagcagcccc
     3241 agcgcccgac aacgacgctg gtggcgaagg ccggcacgaa gatcaggccg gtcgagccgg
     3301 cgataccgcg ccaccgcttg aacagctcga tggcgaggat agcgccagcc agggcgccga
     3361 cgatgctgcg gccaatgccc ggcgcgccgc tgagccagag attgagggtc cctgccccga
     3421 atccgccggc agcggccccc gcaagcagcg ctgcggcata gccgacgccg gcatgctcga
     3481 tcttctggcc ggcgtccgtc aggcgccagc gatagacaag cgcggtgacg gtcatcgccg
     3541 ccaccgccgc cgcaaggtca aagacggtgt ggaggatcac gaccgccaga tggtgccgag
     3601 cgcgttgtaa gcaaagagca gcatgccgag caggaagccg atcgagccga tggcggcacc
     3661 gggctcgacc gccggattgc cgccatagag aaagatgagc gacacgcaca tgagcacggc
     3721 gctcagtgcc gtcacccaga actgcaccct ggccatgacg gacaggttga gcgcgggaaa
     3781 gtggtggtag aaaagtccca tcagtgccat cgagacccag cctgccagca tgatgtgggc
     3841 atgggtcgga tgctgaccat ggtcaccgct gatggccatg tgcagcccca gcgccatgcc
     3901 ggcgagcgca aacagaattg ccgtggttaa gaataggcgt gcgacaccct gcatcttcgt
     3961 ttctcccagc tgcagtgatt ccccctcaca cggggacacc actagcaccg ggcagcaggt
     4021 tgttccactt cgctaagctt gtatttccag tgcgtcgcgc aggatcttct gcaggtcctc
     4081 gcggcgatag ggtttcatga tgat
//
LOCUS       DBNGVR010000321         3358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1691571, whole genome shotgun sequence.
ACCESSION   DBNGVR010000321 DBNGVR010000000
VERSION     DBNGVR010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1691571"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="AC16A0_06815"
     CDS             complement(<1..745)
                     /locus_tag="AC16A0_06815"
                     /inference="COORDINATES: protein motif:HMM:NF023354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HIR3105081.1"
                     /translation="MAAPRHRPYRQAGAAFQIGLRPMPPETWLETGPDHAAFMAAKRQ
                     RLAGLPPQFFHAMPESRAAQAELRDAVVAHLLAQHTEHFTRSGDVLEDRIDGSRHDLA
                     DAMPLALLGRILEEDFILFQTIDGQDLITAACNAYTSSGRIVSSVGRGMHFAHDPVPG
                     LNEQLGPRIDRVLANVQVGTPVVRFNWFLTPISARLFPEGSHDANVAMARRTAARLAD
                     DHRRCGELLWIRSERQTFMRLPQTMALAFG"
     gene            complement(759..1382)
                     /locus_tag="AC16A0_06820"
     CDS             complement(759..1382)
                     /locus_tag="AC16A0_06820"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3105082.1"
                     /translation="MYRLLARKGAGSLAVEAMLAECQLPYAVEDLVRDEKGALPESLH
                     RINPRAEVPTLLMPDDSVMTESAAILIHLANLVPEKGLAPAIGTAQRGPYLRWMIFLA
                     TTVYMSDLRYFYPERYTTDQAGTAGIKAFAEAGMLREFAILSGAVGKGPYVLGTQFSA
                     VDIYAAMLISWVPDLIQLFATHPNLKTLYDKVVERPRIAPVWRRNEV"
     gene            1472..2419
                     /locus_tag="AC16A0_06825"
     CDS             1472..2419
                     /locus_tag="AC16A0_06825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3105083.1"
                     /translation="MEAPLSPAQFMPRSTALGRPKKVRFSYSTADQPFVQRAVIQAIE
                     KFGGQRKLKRIYLQHQKSVKAGENFFDAAIRLLRMRVEFDAAALAATPATGSVLFIAN
                     HPYGVLDGITLTWLATQVRPDTKVLANDVLCQVPEAASNLLPVAFAPTREARETNVQS
                     RRQAQDWLRGGHAIGIFPGGGVSTSEKPLKGPAVDLPWAPFTAKLLRMSKATVVPIYF
                     VGQNSRLFQMASHTSLTLRLSLVFRETARRIGSRLQVRVGRPIPFSEIAHIEDRAELV
                     QELRKRTFGLAQPEDIKGRNNDLHLREAHIKGGKPRDGD"
     gene            complement(2416..3186)
                     /gene="tam"
                     /locus_tag="AC16A0_06830"
     CDS             complement(2416..3186)
                     /gene="tam"
                     /locus_tag="AC16A0_06830"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HIR3105084.1"
                     /translation="MNDWQPAQYLAFADERTRPARDLLARLPLDQPRRIYDLGCGPGN
                     STELLVEAFSGAEIIGIDTSPAMLAAARKAQPYLAFEEGDLATWSPPKPADLLFSNAA
                     LQWVPRHLDVMERLLRSLAPGGVLAVQMPNNLDEPSHRLMAEAAAAGPWAAKLAAAGA
                     ARDPLPAPEDYYRRLKPFCAMLDLWQTTYCHPLRGTACIVDWLKTTGLRPYLAPLTAD
                     EQSGFLAAYETRLVQAYPKLDGEMVLLRFPRFFLMAQA"
BASE COUNT          601 a         1063 c         1149 g          545 t
ORIGIN      
        1 tgccgaaggc gagtgccatg gtctgcggca ggcgcatgaa ggtctggcgc tcggacctga
       61 tccagagaag ctcgccgcag cggcggtgat cgtcagccag ccgtgcggcg gtccgccgag
      121 ccatggcaac gttggcgtca tgcgagcctt ccgggaagag ccgcgccgag atgggcgtga
      181 ggaaccagtt gaagcgtacc actggggtgc cgacctgcac gttcgccagc acgcggtcga
      241 tgcgcgggcc aagctgttca ttgaggcccg gcacgggatc atgggcaaaa tgcatcccgc
      301 ggcccaccga cgaaacgatt cgtcctgaac tggtataggc gttgcaggcg gctgtgatga
      361 gatcctgacc atcgatcgtc tggaacagga tgaaatcctc ctcgaggatc cggccaagca
      421 gggccagggg catggcatcc gcgagatcgt ggcggctgcc gtcgatgcgg tcctccagaa
      481 catcgccgct ccgggtgaaa tgctccgtat gctgagcaag aagatgcgca accacggcat
      541 cgcgcagttc cgcctgggcc gcccgcgact ccggcatggc atggaagaac tgcggcggca
      601 ggcctgccag cctctggcgc ttggcagcca tgaaggccgc atggtcgggc ccggtctcga
      661 gccaggtttc gggcggcatg gggcgaaggc cgatctggaa ggcagccccc gcctggcggt
      721 aaggccggtg gcggggagcc gccatggccg ctgcgccgtc agacctcatt gcgccgccac
      781 accggcgcga tgcgtggccg ctcgacgacc ttgtcataga gcgtcttgag attggggtgc
      841 gtcgcgaaga gctgaatgag gtccggcacc caggagatga gcatggcggc atagatgtcg
      901 acggcgctga actgcgtgcc gagcacgtag gggcccttcc ccacggcgcc cgacaggatc
      961 gcgaattcgc gcaacatgcc agcttcggca aaggccttga tgccggctgt gccggcctgg
     1021 tcggtggtgt agcgctcggg atagaagtag cgcaggtcgc tcatatagac ggtggtggcg
     1081 aggaagatca tccaacgcag atagggaccg cgctgcgccg tcccgatggc cggcgcgagg
     1141 cccttttcag ggacaagatt cgcaaggtga atcaggatgg cggcgctctc cgtcatcacg
     1201 ctgtcatccg gcatgagcag ggtcggaacc tcggctctgg gattgattcg atgcaggctt
     1261 tctggaagcg cccccttctc gtcacgcacg aggtcttcga ccgcataagg cagctggcat
     1321 tcggccagca tggcctcgac cgccagggat ccggcaccct tgcgggcaag caatctgtac
     1381 attcggcacc tcctgaagca ttcaccatac aattctggga cttccgctga gaagggcctt
     1441 gtctccgccg caaatgcatg gaaagcctga cgtggaagca cccctgtcgc cagcgcagtt
     1501 catgcccaga tccaccgccc tcggacggcc caagaaggtt cggttcagct attcgacagc
     1561 ggaccagcct ttcgtgcagc gtgcggtgat ccaggccatc gagaagttcg gcggccagcg
     1621 caagctgaag cgcatctatc tgcagcatca gaagtctgtg aaggccggcg agaacttctt
     1681 cgatgccgcc atccgcctgc tgcgcatgcg ggttgagttc gacgcggccg ccctggcggc
     1741 gacgccggca acggggtccg tgctcttcat cgccaaccac ccctatggcg tgctcgacgg
     1801 gatcacgctg acctggcttg caacgcaggt gaggccggac accaaggtgc tggccaatga
     1861 cgtgctgtgc caggtcccgg aagccgccag caatcttctg ccggtggcct tcgcccccac
     1921 gcgcgaggcc cgcgagacca atgtgcagtc gcggcggcag gcgcaggact ggctgcgcgg
     1981 cggacatgcc atcggcatct ttcccggcgg cggcgtatct acgtcggaaa agccgctgaa
     2041 gggcccggcg gtggatctgc cctgggcccc cttcaccgca aagctgctgc gcatgtccaa
     2101 ggccacggtg gtgccgatct atttcgtggg ccagaactcg cgcctgttcc agatggccag
     2161 ccataccagt ctgacgctgc gcctgtcgct ggtcttccgc gagaccgcgc gccggatcgg
     2221 gtcgcggctg caggtgcgcg tcggccggcc catccccttt tccgagatcg cccatatcga
     2281 ggaccgcgcc gaactggtgc aggaactgcg caagcggacc ttcggactgg cgcagccgga
     2341 agacatcaag ggccgcaaca acgaccttca cctgcgcgag gcccacatca agggcggcaa
     2401 gccgcgcgac ggcgattagg cctgcgccat caggaagaac cgggggaagc ggagaaggac
     2461 catctcccca tcgagcttcg gataggcctg caccaaacgc gtctcatagg cagcgagaaa
     2521 gcccgactgc tcgtccgctg tgagcggtgc cagatagggc ctgaggccgg tggtcttcag
     2581 ccagtcgacg atgcatgccg tgccgcgcag cggatggcag taggtggtct gccagagatc
     2641 gagcatggcg cagaagggct tcagcctccg gtaatagtcc tccggcgcgg gcagcggatc
     2701 gcgcgccgcg ccggcggcgg ctagcttcgc cgcccagggc ccagcggcgg cggcctcggc
     2761 catcaggcgg tgcgagggtt catccagatt gttgggcatc tgcaccgcaa gcacgccgcc
     2821 cggtgcaagc gaccgcagca ggcgctccat cacatcgaga tggcgcggca cccattggag
     2881 cgcggcattg gaaaagagca gatccgctgg cttcggtggc gaccaggtgg cgaggtcgcc
     2941 ctcttcgaag gccagatagg gctgcgcctt gcgcgcggcc gcgagcatgg ccggcgaagt
     3001 gtcgatgccg atgatctcgg cgccggaaaa ggcctcgacc agcagttcgg tggagtttcc
     3061 gggcccgcag ccaaggtcat agatccgccg gggctggtcg agcggcaggc gggccagaag
     3121 atcgcgggca ggccttgtgc gctcgtctgc gaaggcgaga tactgcgcgg gctgccagtc
     3181 attcatgcgt agggtcgtct ccggatttcg tcgtacggcc tcccggcctc aatccgcatg
     3241 gcgatctccg gcggcaggtt caggtcgcgg agggcccggg cggaaagtgt caggcggccg
     3301 agatccggta tcgcatcgcg ccggtgaggc cgcaatgcca gattttcgat cacgagca
//
LOCUS       DBNGVR010000322         1955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4835826, whole genome shotgun sequence.
ACCESSION   DBNGVR010000322 DBNGVR010000000
VERSION     DBNGVR010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4835826"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="AC16A0_06835"
     CDS             <1..401
                     /locus_tag="AC16A0_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3105085.1"
                     /translation="KSKAADFVGTLDVANRMYATLGPTLEKYNVLVCPTTALPAVPAD
                     FDQSKDKLKINGKPLSNVSLGWVMTTPFNTMSRCPVLSMPSGRAKNGIPTGIQIVGRT
                     YCDADVFRAALAYETAQGPWFTDAAHRPAL"
     gene            401..1042
                     /locus_tag="AC16A0_06840"
     CDS             401..1042
                     /locus_tag="AC16A0_06840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3105086.1"
                     /translation="MLEQVIARHDLRRGGAPPGRSKGVVLLIHGRGATAESILPLGEV
                     LAFPDLTYLAPQAEGYTWYPQSFMVPAAANEPFLSQSLRRITEIIADILASGTIPERL
                     AVIGFSQGACLAAETVLRNPRPYGFIGILSGGAIGPPGTPHDYAGSLSGVRVFLGCSD
                     HDPHIPLARVKESTVAFGRMGAQVTERIYPGSSHGINDDEITNLRAGLAPLAA"
     gene            1102..>1955
                     /locus_tag="AC16A0_06845"
     CDS             1102..>1955
                     /locus_tag="AC16A0_06845"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3105087.1"
                     /translation="MIDRRAFLASLFAVALPPSLRAGDFPEPMLEAVADGLDRPWSFA
                     FLPDGAVLVTEKAGALRSIGGDRKLSEPVAGLPDIDADGQGGLLDIALHPGFEQTRLV
                     FLSFTEPREGGMNATSVMRGRLSEDLRSITEVKVIFRQQPAATGGHHFGSRLAFDRTG
                     ALFVTTGDRNSLRGLVQEPDNHIGKIIRITEDGGIPPDNPRREGWLPEIWSIGHRNLQ
                     GAAIHPETGRLWTTEHGAKGGDELNQPEAGKNYGWPDVTYGREYSGKTIGAGLTEKDG
                     IEQPVHYW"
BASE COUNT          340 a          628 c          632 g          355 t
ORIGIN      
        1 agaagtcgaa ggcggccgac ttcgtgggaa cactcgatgt ggccaacagg atgtatgcga
       61 ccttgggacc cacactcgag aagtacaatg tgctcgtctg tccgacgacg gcattgccgg
      121 ccgtgcctgc ggacttcgat cagtcgaagg acaagctcaa gatcaacggc aagcccctgt
      181 ccaatgtgtc gcttggctgg gtcatgacca cgcccttcaa cacgatgagc cgatgcccgg
      241 tcctgtcgat gccgtcggga cgggcgaaga atggcattcc caccggcatc cagatcgttg
      301 gccgcaccta ttgcgatgcc gatgtattcc gcgcagcgct cgcctacgag actgcgcagg
      361 gcccgtggtt caccgacgct gcgcatcgtc cggcgctgtg atgctggaac aggtcatcgc
      421 gcgccatgac ctgcggcgcg gcggcgcgcc gccaggacgc agcaagggtg tcgtgctgct
      481 gatccatggc cggggcgcga cagcggagag cattcttccc ttgggcgagg tgctggcatt
      541 tccggacctc acctatttgg cgccgcaggc ggagggctac acgtggtacc cgcagagctt
      601 catggtgccg gcggcagcca acgagccctt cctgtcacag tcgctccggc gcatcacgga
      661 gatcatcgcg gatatcctcg cgtccggcac gataccggag cggctggccg tcatcggctt
      721 ctcgcagggg gcttgccttg ccgccgagac ggtgctgcgc aatccgcggc cctatgggtt
      781 catcggcatt ctgagcggcg gcgccatcgg tccgcccgga accccgcacg actatgcggg
      841 gtcgctctcc ggtgtccgcg tgtttctggg ctgttcggac catgatcccc atattccgct
      901 ggctcgtgtc aaagagagca cagtggcttt cggccgcatg ggcgcgcagg ttacagagcg
      961 aatctatccg ggcagctcgc atggcatcaa tgacgacgaa atcacaaatc tgagagcggg
     1021 cctcgcgccc ctcgctgcat aaagtccagc ggtgcgcccg ccgcgtaggg aacatcattg
     1081 ttgtcatcaa gcgggtgttt catgatcgat cgccgtgcct ttctggcaag cctgttcgcg
     1141 gtagcgctgc ccccttccct gcgggcgggt gatttccccg agcccatgct cgaagccgtg
     1201 gccgacggcc ttgaccggcc gtggtccttc gccttcctgc cggacggagc cgttctcgtc
     1261 accgagaagg caggagccct ccgctccatc ggtggcgacc gaaagctttc ggagccggtt
     1321 gcaggcctgc cggacatcga tgccgatggc cagggcgggc tgctcgacat tgccctccat
     1381 cccggtttcg agcagacccg gctggtcttc ctctccttca cggagccgcg tgagggtggg
     1441 atgaatgcaa ccagcgtgat gcgcggcagg ctgtcggagg atcttcgaag catcacggag
     1501 gtcaaggtca tcttccgcca gcagccggcg gccacgggcg gccatcactt cggttcgcgc
     1561 ctcgcttttg accgcacggg cgccctcttc gtcaccacgg gcgaccgcaa cagcctgcgc
     1621 ggattggtcc aggaaccgga caatcatatc ggcaagatca tccgcattac cgaggatggc
     1681 ggcattccgc ccgacaaccc caggcgtgag ggatggctgc ccgagatctg gtccatcggc
     1741 caccgtaacc tgcaaggcgc agcgatccat cccgagacgg gcaggctctg gaccaccgag
     1801 catggggcca agggtggcga cgagttgaac cagcctgagg cgggcaagaa ttatggctgg
     1861 cccgatgtca cttacgggcg cgagtattcg ggcaagacga tcggcgccgg gctgaccgag
     1921 aaggatggga tcgagcagcc cgtccactat tggga
//
LOCUS       DBNGVR010000323         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_513347, whole genome shotgun sequence.
ACCESSION   DBNGVR010000323 DBNGVR010000000
VERSION     DBNGVR010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_513347"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="AC16A0_06850"
     CDS             complement(<1..515)
                     /locus_tag="AC16A0_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3105088.1"
                     /translation="MSELRKVSPLARDAMAYVLAGGRGSRLMEMTDKRAKPAVPFGCK
                     SRIIDFALSNALNSGIRRIGVATQYKAHSLIRHMQRGWNFFRPERNESFDILPASQRV
                     SEDQWYAGTADAVYQNLDIIMSYGPKYMVILAGDHIYKMDYELMLQQHVDSGADVTIG
                     CLEVPRKEATG"
     gene            complement(548..>2196)
                     /gene="glgB"
                     /locus_tag="AC16A0_06855"
     CDS             complement(548..>2196)
                     /gene="glgB"
                     /locus_tag="AC16A0_06855"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR3105089.1"
                     /translation="TIGQGYKFELLDSGGRLLPLKSDPFGFHAEIRPKTASRVAETGN
                     FVWNDEAYIEARRHRDPRRMPMSIYEVHLGSWKRKDGNAFLSYDELAADLIPYVADMG
                     YTHIELLPITEHPFDPSWGYQPTGLYAPTSRFGDPAGFARFVDAAHRSGIGIILDWVP
                     AHFPTDEHGLARFDGTALYEHEDPRQGFHPDWNTAIYNFGRKEVAGFLVNNALFWLER
                     YHLDGLRVDAVASMLYLDYSRKDGEWIPNRHGGRENLEAIAFLQRMNHESYGQNPGVV
                     TIAEESTAFPGVSQPSHTGGLGFGFKWNMGFMHDTLEYLKREPIYRQHHHNELTFGLL
                     YAFSENFVLPLSHDEVVHGKGSLLAKMAGDDWQKFATLRAFYAFMWGYPGKKLLFMGQ
                     EMAPWTEWSEARSLDWHLLDHAPHKGMQSLVRDLNRHYRALPALHARDCEPEGFDWLI
                     ADDNQNSVYAWARYSGGGDPAVVIVSNFTPVPREDYVLPLPRAGRWREVLNSDAAAYG
                     GGGLGNMGEIQADAVPSHGKPASAKVLLPPLATLYFLFDA"
BASE COUNT          414 a          657 c          722 g          403 t
ORIGIN      
        1 aagccggtgg cctccttgcg cggcacctca aggcacccga tggtgacgtc ggcgcccgag
       61 tcgacatgct gctgcagcat cagttcgtag tccatcttgt agatgtggtc gccggccagg
      121 atcaccatgt atttcgggcc gtagctcatg atgatgtcga ggttctggta gactgcgtcg
      181 gccgtgccgg cataccactg gtcttccgag acgcgctgcg aggcgggcag gatgtcgaag
      241 ctctcgttgc gctcggggcg gaagaagttc cagccgcgct gcatgtggcg gatcaggctg
      301 tgcgccttgt actgcgtggc gactccgatg cggcggatcc ctgagttcag ggcattggac
      361 agcgcgaagt cgatgatgcg ggacttgcag ccgaaaggca cggcgggctt ggcccgcttg
      421 tcggtcattt ccatgaggcg gctgccgcga ccgccggcca gcacataggc catggcatcg
      481 cgggccagtg gcgaaacctt gcgcaactcc gacatcgata ctccctcccg gtactggtct
      541 tcttttctta tgcgtcaaac aggaaataca aagttgccag cggcggcaag aggactttcg
      601 cggaggccgg cttgccatgg ctgggcaccg catcggcctg gatttccccc atattgccaa
      661 gcccgccgcc gccataggct gcggcatcgc tgttcagcac ctcgcgccag cggccggccc
      721 ggggcagggg cagcacataa tcctcgcgcg gcacgggggt gaaattggag acgatcacca
      781 ccgctggatc gccgccgcca gaatagcggg cccaggcata gaccgagttc tggttgtcgt
      841 cggcgatcag ccagtcgaaa ccttccggtt cgcaatcgcg ggcatgcagg gccggaagcg
      901 cgcggtaatg acggttgagg tcgcgcacaa gggactgcat gcccttgtgc ggggcgtgat
      961 caagcaggtg ccagtccagg ctgcgggcct ccgaccattc cgtccagggt gccatctcct
     1021 ggcccatgaa cagcagcttc ttgcccggat agccccacat gaaggcgtag aaggcgcgca
     1081 gcgtcgcgaa cttctgccag tcatcgccag ccatcttggc gagcagcgag cccttgccgt
     1141 gcactacctc gtcgtgcgag agcggcagca cgaagttctc ggaaaaggca tagagcaggc
     1201 cgaaggtcag ctcgttgtga tgatgctggc ggtagatcgg ttcgcgcttc agatactcca
     1261 gcgtgtcgtg catgaagccc atgttccact tgaagccgaa gccgagaccg cccgtgtgcg
     1321 acggttgcga gacgccggga aaggcggtcg actcctcggc gatggtcacg acgccagggt
     1381 tctggccata ggattcgtga ttcatgcgct gcaggaaggc gatggcctcg agattttcgc
     1441 ggccgccatg gcgattggga atccactcgc cgtccttgcg cgagtagtcg agatagagca
     1501 tcgaggccac tgcatcgacg cgcaagccgt cgagatgata gcgctcgagc cagaacagcg
     1561 cattgttgac gagaaagcct gccacttcct tgcggccaaa attgtagatc gccgtgttcc
     1621 agtcggggtg aaagccctgg cgcggatctt cgtgctcata gagtgccgtg ccgtcgaagc
     1681 gggccagccc gtgctcatca gttgggaaat gcgccggaac ccagtcaagg atgatgccga
     1741 tgccggagcg atgggccgca tcgacgaagc gggcaaaacc ggccggatcg ccgaagcgcg
     1801 aggtcggcgc atagaggccg gttggctggt agccccagga cggatcgaag ggatgctcgg
     1861 tgatcggtag cagctcgatg tgggtgtagc ccatgtcggc gacataagga atgagatcag
     1921 cggccagttc gtcataactc aggaaggcat tgccgtcctt gcgcttccac gaaccgagat
     1981 gcacctcata gatcgacatt ggcatgcggc gcggatcgcg atggcgccgc gcctcgatat
     2041 aggcctcgtc gttccacacg aagttcccag tctcggccac gcgggacgcc gtcttcggcc
     2101 ggatttcggc gtggaacccg aaaggatcgc tcttcagcgg aagcagccgc ccgcccgaat
     2161 ccagcagttc gaacttgtag ccctgcccga tggtgg
//
LOCUS       DBNGVR010000324         2324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3067343, whole genome shotgun sequence.
ACCESSION   DBNGVR010000324 DBNGVR010000000
VERSION     DBNGVR010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3067343"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="AC16A0_06860"
     CDS             <1..846
                     /locus_tag="AC16A0_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR3105090.1"
                     /translation="IVPPLAIDGPTLTIRKFKKDRLKLPDLVKFGSITPEGATILEIV
                     GRVRCNVLISGGTGSGKTTLLNCLTGYIDHDERVITCEDAAELQLQQPHVVRLETRPP
                     NLEGEGEITMRDLVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANSP
                     REAISRLESMITMGGFSLPSKTIKEMICGSIDVVIQAARLRDGSRRITHITEVVGMEG
                     DVIITQDLFVYEIVGEDANGKIIGRHRSTGIARPHFWDRARYYNEERRLAEALEKAEQ
                     RNEGE"
     gene            871..1881
                     /locus_tag="AC16A0_06865"
     CDS             871..1881
                     /locus_tag="AC16A0_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3105091.1"
                     /translation="MYFGDDLAQLAFIAMVVIAVGGVALALFYPMLSDRSASQRVKAI
                     AESKKGGAQKGGTFSKFMDSQKDSRRKQVQESLKQIEERAKQRNKRLSLRMLIMQSGL
                     DLSIELFWIFSVLLGVVISAGAFFAGAPWYVSVAAALAGTFGLPRWLLGLVRNRRQAV
                     FLNDFADAIDVMVRGLKAGLPVSDAMKIIASESGPPVGPEFLEVVEGQRVGITIDQGI
                     ERMYERMPMSEVNFLGIVMSIQAKTGGNLSEALNNLSKVLRDRKKLKAKIRAVSQEAK
                     SSAAIIGSLPFVIMGALTILNPTYLLPMFTTDIGNVMLVGCGLWMLTGILVMRQMINF
                     EI"
     gene            1898..>2324
                     /locus_tag="AC16A0_06870"
     CDS             1898..>2324
                     /locus_tag="AC16A0_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105092.1"
                     /translation="MLNFLSSLFQIETMITILVGMAAFATVLTVTAPFLETDRLKARM
                     KSVGQERDRLRAAQRAQLVTGESAKLRDRVNKNIATQLVEGLNLRKIFEAESSRNLLR
                     QAGLRSERHLVTYLAARFAVPLVLGLLVFIYSSTLYADKV"
BASE COUNT          437 a          750 c          695 g          442 t
ORIGIN      
        1 atcgttcccc cgctcgctat cgacgggccc actctcacca ttcgtaagtt caagaaggac
       61 cgcctcaagc ttcccgatct ggtgaagttc gggtcgatca cgccggaggg cgcaacgatc
      121 ctcgagatcg tcggccgggt ccgctgcaac gtgctgatct ctggtggtac cggttccggc
      181 aagaccacgc tactcaactg ccttaccggc tacatcgatc acgacgagcg cgtcatcacc
      241 tgcgaggacg ccgccgaact tcaattgcag cagccgcatg tggtgcgcct cgaaacccgc
      301 ccgcccaacc tcgagggcga gggtgagatc accatgcgcg acctggtgaa gaactgcctt
      361 cgtatgcgtc ccgagcggat catcgtcggc gaagtccgcg gaccggaggc cttcgacctt
      421 ctgcaggcga tgaacacggg ccatgacggt tccatgggca cgctccacgc caacagtccg
      481 cgcgaggcca tctcgcgtct cgagtcgatg atcaccatgg gcggcttctc gctgccgtcc
      541 aagaccatca aggaaatgat ctgcggatcg atcgacgtcg tcatccaggc cgcccgcctg
      601 cgcgacggtt cgcgccgcat cacccacatc accgaagtgg tcggcatgga aggcgatgtc
      661 atcatcaccc aggacctctt cgtctacgag atcgtcggcg aggatgcgaa tggcaagatc
      721 atcggccggc accggtcgac cggcatcgcg cgcccgcact tctgggatcg tgcgcgctac
      781 tacaatgaag aacgccgcct ggcagaagct ctcgaaaagg ccgagcagcg caacgaaggc
      841 gagtagtgac tgagggaggc ctgaaggctc atgtatttcg gagatgatct ggcccagctt
      901 gccttcatcg cgatggtcgt cattgccgtc ggcggcgtgg cccttgccct gttctacccg
      961 atgctctcgg accgcagtgc cagccagcgc gtaaaggcca ttgcggaatc gaagaagggt
     1021 ggcgcgcaga agggcggcac cttctcgaaa ttcatggata gccagaagga cagccggcgc
     1081 aagcaggtac aggaaagttt aaagcagatc gaggagcgcg ccaagcagcg caacaagcgc
     1141 ctatcgctcc gcatgctgat catgcagtcg ggcctggacc tcagtatcga gctcttctgg
     1201 attttctccg ttctgctcgg tgtcgttatc tcggccggcg ccttctttgc cggcgcccca
     1261 tggtatgtct cggtcgctgc cgcgctggcc ggtaccttcg gcctgccgcg ctggctgctt
     1321 ggcctggtcc gaaatcggcg ccaggccgtc ttcctcaacg acttcgccga tgccatcgac
     1381 gtcatggtgc gcggactcaa ggccggcctt ccggtgtcgg acgccatgaa gatcattgcc
     1441 tccgagtcgg gtcccccggt cggacccgag ttcctcgaag tggtggaagg ccagcgcgtc
     1501 ggtattacca tcgaccaggg cattgagcgc atgtacgaac gcatgcccat gtccgaggtg
     1561 aacttcctgg gcatcgtcat gtcgattcag gccaagaccg gcggcaacct ctcggaagca
     1621 ctcaacaacc tgtcgaaggt gttgcgtgac cgcaagaagc tcaaggccaa gatccgcgcc
     1681 gtcagccagg aggccaagtc ttcggctgcc atcatcggct ccttgccctt cgtcatcatg
     1741 ggcgcgctga ccattctcaa tccgacctat ctgctgccga tgttcaccac ggatatcggc
     1801 aatgtcatgc ttgtggggtg cgggctctgg atgctcaccg gaatcctggt gatgcgccag
     1861 atgatcaact tcgaaatcta ggcgggctgt agcacacatg ttgaatttcc tgtccagcct
     1921 ctttcagatc gagaccatga tcaccatcct ggtgggcatg gcggcgttcg cgaccgtcct
     1981 gaccgtgacg gcgcccttcc tcgagaccga ccgcctcaag gcgcgcatga agtcggtggg
     2041 ccaggaacgc gacaggctgc gcgcggcgca acgggcccag ctcgtgaccg gcgagagcgc
     2101 aaagctgcgc gacagggtca acaagaacat cgccacgcag ctggtggagg ggctcaacct
     2161 ccgcaagatc ttcgaggccg agtcctcgcg gaacctgctg cgccaggcgg gtctgcgcag
     2221 cgaacgccac ctggtgacct atctcgccgc gcgtttcgcc gttccgctgg tgcttggcct
     2281 gctcgtcttc atctattcct ccacgctcta tgccgacaag gtcg
//
LOCUS       DBNGVR010000325         1786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2085982, whole genome shotgun sequence.
ACCESSION   DBNGVR010000325 DBNGVR010000000
VERSION     DBNGVR010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2085982"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1786
                     /gene="glnD"
                     /locus_tag="AC16A0_06875"
     CDS             <1..>1786
                     /gene="glnD"
                     /locus_tag="AC16A0_06875"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR3105093.1"
                     /translation="DLLFVLPQRQSPRVQSIAEFILYTLWDTRQKVGHATRNIEECIR
                     LAKTDTTILTAILEARYICGDVGVFERLVHDFRRDIVAKGARDFVAGKLAERDLRLVK
                     SGESRYLVEPDVKDGKGGLRDLNTLFWIAKFLYATNATDELAEKGAFTREELAIFKKC
                     ENFLWAVRCHLHFLAGRGDDRLSFDRQSEIAERLGYKAHGGLKHVERFMKHYFLVAKD
                     VGDLTRILCASLEARQVKDAPTLSRMLGRLRPRGTPSFADAPDFRLESGRIVAKDADA
                     FSRDPVNMIRLFAIASRQGAEIHPDALKLVRKSLPLIRKSVCNDPQANAIFLEMLTQG
                     SEPETILRMMNEAGVLGRFIPEFGRIVAMMQFNMYHHYTVDEHLIRAVGILAQIDRGQ
                     LSEDHPLAASVFPTVTSKRALYVAVLLHDIAKGRKEDHSIAGERIALELCPRFGLSAA
                     ETETVAWLIRHHLLMSETAQMRDLNDFKTILDFAGVVQSFERLKLLLILTVTDIRAVG
                     PGVWNGWKGQLLRTLYAESEPVLSGGHTAVSRRDRVAEAQAAFFESITDWSADQKKAY
                     GARHYDAYWLSVNTAKQLKHRTLIASAPP"
BASE COUNT          325 a          580 c          552 g          329 t
ORIGIN      
        1 gacctgctct tcgtgttgcc gcagcgccag tcgccgcggg tccagagcat cgccgagttc
       61 atcctctaca cgctatggga cacgcgccag aaggtgggcc acgcgacccg caacatcgaa
      121 gaatgcatcc ggctggccaa gaccgatacc acgatcctca ctgccattct cgaggcgcgc
      181 tacatctgcg gcgacgtcgg cgtcttcgag cgcctggtgc atgatttccg gcgcgacatc
      241 gtggccaagg gcgcgcggga cttcgttgcc ggcaagctgg ccgagcgtga cctccgcctc
      301 gtcaagtcgg gcgagagccg ctatctggtc gagcccgacg tgaaggatgg caagggcggc
      361 ctgcgcgatc tcaacacgct gttctggatc gccaagttcc tctacgccac caacgccacc
      421 gacgagctcg ccgaaaaggg cgcctttacc cgcgaagagc tggcgatctt caagaagtgc
      481 gagaactttc tctgggcggt cagatgccac ctgcactttc tggccgggcg cggcgacgac
      541 cggctgagct ttgaccgcca gtccgagatc gccgagcggc tcggctacaa ggcgcatggc
      601 ggcctgaagc atgtcgaacg cttcatgaag cactacttcc tcgtggccaa ggacgtgggc
      661 gatctcacgc gtatcctttg cgccagcctg gaggcccggc aggtgaagga tgcgcccacg
      721 ctcagccgca tgctgggccg cctccgtccg cgcggcacgc cgagctttgc cgacgccccg
      781 gacttccgcc tcgaatccgg ccgcatcgtg gccaaggatg cggatgcctt ctcgcgcgat
      841 ccggtgaaca tgatcaggct cttcgccatc gccagccgcc agggcgcgga gattcatccg
      901 gatgcgctga agctggtccg caagtcgctg ccgctgatcc gcaagagcgt gtgcaacgac
      961 ccgcaggcca atgcgatctt cctcgagatg ctcacccagg gaagcgagcc ggaaaccatt
     1021 ctccgcatga tgaacgaggc cggcgtgctg ggccgcttca ttccggagtt cggccgcatc
     1081 gtggcgatga tgcagttcaa catgtatcat cactatacgg tggacgagca tctgatccgt
     1141 gccgtcggca ttctggcgca gatcgaccgc ggccagctct cggaggatca cccgcttgcc
     1201 gcttctgtct tcccgacggt caccagcaag cgcgcgctct atgtggccgt gctgctgcac
     1261 gacatcgcaa agggccgcaa ggaggatcac tccatcgcag gcgaacggat tgcgctggag
     1321 ctctgccccc gctttggcct ttcggcggct gaaaccgaaa ccgttgcctg gctgatccgt
     1381 catcacttgc tgatgagcga aaccgcgcag atgcgtgatc tcaacgactt caagaccatt
     1441 ctcgattttg ccggggtcgt gcaaagtttt gaacggctca agctgctgct gatcctgacc
     1501 gtcaccgata tccgcgcggt ggggcctggt gtctggaatg gatggaaggg tcaattgttg
     1561 cggaccctct atgccgaatc ggagcccgtc ctgtcgggcg gtcataccgc cgtctcgcgc
     1621 cgcgatcgcg ttgccgaggc acaggcggcg ttcttcgaga gcatcaccga ctggagcgcc
     1681 gaccagaaga aggcctatgg tgcgcgccac tatgatgcct attggctgag cgtgaacacg
     1741 gcgaagcaat tgaagcaccg gacgctgatt gccagtgcgc cgccca
//
LOCUS       DBNGVR010000326         2425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_912627, whole genome shotgun sequence.
ACCESSION   DBNGVR010000326 DBNGVR010000000
VERSION     DBNGVR010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_912627"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="AC16A0_06880"
     CDS             complement(<1..1225)
                     /locus_tag="AC16A0_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3105094.1"
                     /translation="MFGLRGLAAKIFGTTNDRKVTKYKPRVDEINALEPALAALSDAE
                     LRARTQTFKDEVAAGKSLDDLLVPAFATVREASKRTLGLRHFDVQLIGGMVLHEGMIA
                     EMKTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLARRDAAWMGEVYGFLGLTTGVIV
                     HGLSDEERRAAYASDVTYATNNELGFDYLRDNMKYHLAEMVQRGHNFAIVDEVDSILV
                     DEARTPLIISGPVDDKSDLYNTVNGFIPELSPEDYELDEKQRSCTLTEKGNVHLEQVL
                     GAAGLLKGENLYDAENVTIVHHVQQALRAHKLFQRDKDYIVKDGQVIIIDEFTGRMMP
                     GRRYSEGLHQALEAKEHVDIQPENVTLASITFQNYFRLYKKLAGMTGTAATEAEEFQD
                     IYRLEVVEIPTNVSVA"
     gene            1564..>2425
                     /locus_tag="AC16A0_06885"
     CDS             1564..>2425
                     /locus_tag="AC16A0_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3105095.1"
                     /translation="MLRLKTILRTSLLASALAVAWPLAGSAQEAKEDKVIAIVNGHEI
                     RTSEVQMATDDIVGQLPDLPPKLRYPFVVEYLIERHLLAQYAVKEGIAETDEYKRRLA
                     LYQAKALRDAYFFQTIRPQVTEEMIKAAYDEEAAKVAQTERIRARHILLGTEKEAQDI
                     LDRLNKGEDFAALAKQYSLDGSKDYGGDLGYFTAPEMVVEFSTAAFALKAGEISQPVK
                     TDFGWHIIKLEDRKMGAAQPYDQVKSAIRNVLLRRKVQETMDKIRAASKVEIVDEDLK
                     KYAEDAAKAAK"
BASE COUNT          457 a          709 c          771 g          488 t
ORIGIN      
        1 gggcgacgga gacattggtc gggatttcga cgacctcaag ccggtagatg tcctggaatt
       61 cttcggcttc ggttgccgcc gtgccagtca tgccggcgag cttcttgtag aggcggaagt
      121 agttctggaa ggtaatggaa gcgagcgtca cattctccgg ctggatgtcg acatgctctt
      181 tcgcctccag cgcctgatgc aagccttccg agtagcggcg gcccggcatc atgcggccgg
      241 taaactcgtc tatgatgatg acctggccgt ccttgacgat gtagtccttg tcgcgctgga
      301 acaacttgtg ggcgcgcagc gcctgctgca cgtgatgcac gatggtcacg ttctcggcat
      361 cgtagaggtt ttcgcccttc agcaggccgg ccgcaccgag cacctgttca agatggacat
      421 tgcccttctc ggtcagcgtg caggagcgtt gcttctcgtc gagctcgtag tcttccggcg
      481 agagctccgg aatgaagccg ttgacggtgt tgtagagatc cgacttgtcg tccaccgggc
      541 cggagatgat gagcggggtg cgggcctcgt ccaccaggat cgagtcgacc tcgtcgacga
      601 tcgcgaaatt gtgcccacgt tgtaccatct cggccagatg atacttcatg ttgtcgcgca
      661 gatagtcgaa gccgagttcg ttgttggtgg cgtaggtcac gtcgctggca taggcggccc
      721 ggcgttcctc gtcggacagg ccgtggacga tgacccccgt ggtcagtccc aggaagccgt
      781 agacctcacc catccaggcg gcgtcgcggc gggcgaggta gtcgttcacg gtcaccacat
      841 gtacgccctt gccggcgagt gcattgagat agaccgcgag cgttgcgacg agagtcttgc
      901 cttcaccggt tttcatctcg gcgatcatgc cttcgtgcag caccatgccg ccaatcagct
      961 gaacgtcgaa atgccgcaaa cccagggtgc gcttggaagc ttcgcgcacc gtggcgaagg
     1021 ccgggacgag gaggtcgtcg aggctcttgc cggcggccac ttcgtctttg aaagtctggg
     1081 tccgggcgcg gagttcggcg tccgacaggg ctgccagcgc cggctccagc gcgttgattt
     1141 catcaaccct tggcttgtac ttggtaacct tgcgatcgtt ggtggtgcca aagatttttg
     1201 cggccaggcc gcgcagtccg aacatgtgat tcgcatcctt caggggaggg cgataggccc
     1261 cgggaaatgc cgctttctag tcaagttgtc cggcgggggc aattggccgt ctcgtggcac
     1321 gttccgacat aggcccatgg cctcgccatt tcaatgtgtt gcatctgggc aacagctctt
     1381 gcaatccgcc atttgcgcca attccgcccc ttgcaagggc tggtgggccg gaccaatgtg
     1441 cgccgtcggt tttcggcccc ggacagattc tccggggggc atattcttgc cttgtttgac
     1501 gcccaagagg cggcggaacg cggttcgaat gtccgtgaac cggacctgca atcggtggat
     1561 ttaatgctgc gacttaaaac gatcctgaga acgtccttgc tggccagtgc gctcgccgtg
     1621 gcctggccgc tggcgggttc cgcccaggaa gcgaaggaag acaaggtcat cgccatcgtc
     1681 aacgggcatg agatccgcac ctccgaggtc cagatggcaa ccgacgatat cgtcggccag
     1741 cttcccgacc tgccgcccaa gctgcgctat cccttcgtgg tcgagtacct catcgagcgt
     1801 catctgctgg ctcagtatgc ggtgaaggaa ggaatcgccg aaaccgacga gtacaagcgc
     1861 cgcctggcgc tctatcaggc caaggccctg cgcgatgcct acttcttcca gaccatccgt
     1921 ccacaggtga cggaggagat gatcaaggca gcctatgacg aggaagccgc gaaggtggct
     1981 cagaccgagc gcatccgcgc ccgtcacatc ctgttgggca ccgagaagga ggcccaggac
     2041 attctcgaca ggctgaacaa gggcgaggat ttcgccgcgc tggccaagca gtacagcctc
     2101 gacggatcga aggattatgg cggcgatctg ggttacttca cggcgcccga aatggttgtc
     2161 gaattctcga ccgcggcctt cgcgctgaag gccggagaaa tctcgcagcc ggtgaagacc
     2221 gatttcggct ggcacatcat caagctggaa gaccggaaga tgggggcggc ccagccctat
     2281 gatcaggtca agtcggccat ccgcaatgtg ctgctgcgcc gcaaggtgca ggagacgatg
     2341 gacaagatcc gtgcagcctc gaaggtcgag atcgtggacg aggatctcaa gaaatacgcc
     2401 gaggatgccg ccaaggcggc caaga
//
LOCUS       DBNGVR010000327         4501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3856350, whole genome shotgun sequence.
ACCESSION   DBNGVR010000327 DBNGVR010000000
VERSION     DBNGVR010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3856350"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..814)
                     /locus_tag="AC16A0_06890"
                     /pseudo
     CDS             complement(92..814)
                     /locus_tag="AC16A0_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017959225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
     gene            complement(804..1049)
                     /locus_tag="AC16A0_06895"
     CDS             complement(804..1049)
                     /locus_tag="AC16A0_06895"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3105096.1"
                     /translation="MADHSDIASLTFEKALAQLEEIVTKLESGKVDLEQSITIYERGE
                     ALKKHCERLLKDAEARIEKIILKPDGTPGGTEPLDVG"
     gene            complement(1049..2029)
                     /locus_tag="AC16A0_06900"
     CDS             complement(1049..2029)
                     /locus_tag="AC16A0_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3105097.1"
                     /translation="MTTLLFTHDSSLRHVTPEGHPERVDRIRAVNQILSSDHFRDLTR
                     RTAPAGREEDILRAHVPEHLRRVKAIAPAEGFEFLDADTAMSPGTLEAALHAVGAATA
                     GVDAVFSGEADNVFCALRPPGHHAESRRAMGFCFFNQAAIAAHYARAAHGAERVAVID
                     FDVHHGNGTQDIFWSDPDLFYGSTHQMPLYPGTGSVHETGAGNIFNAPLRAGDGSAQF
                     RAAMATRVLPALDAFAPDLVIVSAGFDAHHRDPLGSLQLTEEDFAWITLQIMEAAEIH
                     CGGRIVSVLEGGYDLQGLAASVGIHVQALMRGNADQLDDTDDSDAYEDDD"
     gene            2075..3013
                     /locus_tag="AC16A0_06905"
     CDS             2075..3013
                     /locus_tag="AC16A0_06905"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3105098.1"
                     /translation="MLSPAEIEDAARALREGRLVAFPTETVYGLGADATNGRAVAEVY
                     AAKGRPAFNPLIVHVASATQAFDLGQFPDAARRLALAGWPGPLSIVVPRAPDCPVSLL
                     ASAGLDSIALRVPAHPVALALLQATARPVVAPSANPSGRVSPTSADHVRRHLGGKVAM
                     ILDGGRCRVGVESSVVSFLDDGPKLLRPGGMPRAEIEAIIGRPLAVEAHSQRPHAPGQ
                     LSSHYAPRAELRLNAGEPRAGEAYLGFGPLHAHGPYNLSARGDLVEAAAALFGLLHEI
                     DATGVARIAVAPVPHHGLGEAINDRLLRAAAPRGAP"
     gene            3010..4413
                     /locus_tag="AC16A0_06910"
     CDS             3010..4413
                     /locus_tag="AC16A0_06910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3105099.1"
                     /translation="MKPSAATLDRLAAVVGEAHVLRDAPAMDGYMREWRGIWHGVSPL
                     VLRPGSTREVSQILAIANETRTAIVPQSGNTGLNGGQIPRDSGDEVLLSLDRMTQILD
                     IDAIDNTITVEAGCILKSVQDAAAAVDRLFPLSLASEGSCRIGGNLSTNAGGLNVIAY
                     GNARDLCLGLEVVLADGRVWNGLKRLRKDNTGYDLRDLFIGAEGTLGIIPAAVLKLFP
                     RPQRHETAFIAVPGPQAAVELLSLLRAASGNRVVAFELIASLALQFTERHAGLRSPLE
                     SASPWYVLAELADPLPGAFTALLEEAMDKGLVTDAAIAQSDRQRDAFWTIRETISDSQ
                     KPEGGSIKHDIAVPVSQVPRFLAAADAAVARFMPGARLVTFGHLGDGNIHYNVSQPVG
                     MDTQQYLDQWNAMNDVVFEVVTRFGGSISAEHGMGRLKAHHMPLIKSPVELEMMRGLK
                     RLFDPNTILNPGRVLPE"
BASE COUNT          723 a         1560 c         1474 g          744 t
ORIGIN      
        1 gacggcgccg gcgagcatcc tgccggcccg gcccagcgct gcgtcctgta ggcggcaacc
       61 gggctgcctg caatggtccg gcggcgaccc cgcgccctgc ggcgatatgg acttcgcggt
      121 tcgcgtcgcc accggcgatc ggcgatggcg tgatgccgcg cacagaccag ccttggcctt
      181 caaggaaagc cgcgatctcg gcgcagacct gcgcccattc gttttcgtcg cctaccacgc
      241 cgcctttgcc caaggccgcg cggcccactt cgaactgcgg cttgatgagg gcaatcagat
      301 cggcacccgg ctccgccagc gcgagcgccg gcggcagggc gagcctgagc gaaatgaaac
      361 tgacatcgca gacgataagg tcgaaggctt ccggcacatg cgcgcgggtc aggtcgcggg
      421 cattgagacc ttcgacaagc aagatccgcg gatcctcaaa cgccatctgg ctatgaccca
      481 cgtcgatggc ggtaacatgg gccgcccctt cctccagcaa tacctgcgta aagccgcccg
      541 tcgaagcgcc gatgtcgagc gcatgccgtc ccgtcgcggc gatgcggaag tgggcaagcg
      601 catgcttgag tttcagcgcc gcgcgcgaca catagggctt cgctggatct gcgatggcga
      661 tcgcggcgtc ctcggccgtc atcggcgacg gcttcgcggc tgcaaggccg ttgaccgtca
      721 ccgcgccgcg cagaaccgcg tcgcgggccc gcgcgcgggt ctggtagaaa ccgcgcgcca
      781 ccagcagcgc gtcgagacgc tgcctagccg acatccagcg gctcggtacc gcccggcgtg
      841 ccgtccggct tgagaatgat cttctcgatt cgcgcctcgg cgtccttcag aagcctttcg
      901 caatgcttct tcagcgcctc gccgcgctcg tagatggtga tggactgctc gagatcgacc
      961 ttgccggatt cgagcttggt cacgatctcc tcgagctggg caagcgcctt ctcgaaggtg
     1021 aggctggcaa tgtcggaatg atcggccatc agtcatcgtc ctcataggca tcggaatcgt
     1081 cggtgtcgtc gagctggtcg gcattgccgc gcatcagcgc ctggacgtga atccccacgg
     1141 atgccgccag cccctgcagg tcgtagccgc cttcgagtac cgaaacaatg cggccgccgc
     1201 aatgaatctc ggctgcttcc atgatctgca gcgtgatcca ggcaaagtct tcctcggtca
     1261 attgcaggct tcccagggga tcgcggtgat gcgcatcgaa gcctgccgac acgatgacca
     1321 ggtccggcgc aaaggcatcc agcgccggca gcacgcgggt cgccatggcg gcgcgaaact
     1381 gtgcgctgcc gtcgccggcg cgcaggggcg cgttgaagat attgcccgcc cccgtctcat
     1441 gtacagagcc ggtgccggga tagagcggca tctggtgggt cgagccataa aacagatcgg
     1501 gatcggacca gaagatgtcc tgggtgccgt tgccgtggtg cacgtcgaag tcgatcaccg
     1561 ccacgcgttc ggcaccatgg gcggcgcgtg cgtagtgggc ggcgattgcg gcctggttga
     1621 agaagcagaa gcccatggcg cggcggcttt cggcatgatg gcccggcggg cgcagggcgc
     1681 agaagacatt gtcggcctcg cccgagaaca cggcatcgac gccggccgtc gctgccccga
     1741 ccgcatgaag tgccgcctcc agcgtgccgg gcgacatggc ggtgtcggca tcgagaaact
     1801 cgaagccctc ggccggcgcg atcgccttca cgcggcgcag gtgctccggc acatgggcgc
     1861 gcaggatgtc ttcctcgcgt ccggccggcg ccgtacgccg cgtcaggtcg cgaaaatgat
     1921 ctgaactcag gatctggttc accgcgcgga tgcggtccac gcgttccgga tgcccctcgg
     1981 gcgtgacatg acgcagcgac gagtcatgcg tgaacagcag ggtggtcatg gggcgcttgt
     2041 agccgcttcc gcagcggact gctatagagc cgccatgctg agccccgccg aaatcgagga
     2101 tgccgcgcgc gccctgcgcg agggaaggct cgtggccttc ccgaccgaga cggtctatgg
     2161 gcttggcgcc gatgccacca atggccgcgc ggtggccgag gtctatgccg cgaaggggcg
     2221 gcccgccttc aatccgctga tcgtgcatgt cgcatcggca acccaggctt tcgaccttgg
     2281 gcaattcccc gacgccgcgc gccgcctggc cctcgcggga tggccgggac cgctcagcat
     2341 cgtcgtgccc cgcgcgcctg attgccccgt atcgctgctg gcctcggccg ggctcgactc
     2401 cattgccctg cgcgtgcctg cccatccggt ggcgctggcg ctgctgcagg cgaccgcccg
     2461 cccggtggtg gcccccagcg ccaacccctc ggggcgcgtg agccccacct cggcggacca
     2521 tgtgcgccgc cacctcggcg gcaaggtcgc catgatcctg gatgggggac gatgccgggt
     2581 cggcgtggaa tcgagcgtgg tcagcttcct cgacgacggg ccgaagctcc tgaggcccgg
     2641 cggaatgcca cgcgccgaga tcgaggccat cattggcagg cccctggcgg tcgaagcgca
     2701 ttcccagcgg ccgcatgcgc cgggccagct gtcgagccac tacgcgccgc gcgccgaact
     2761 ccgcctcaat gccggggaac cgcgtgccgg cgaagcctat ctgggcttcg ggcccctgca
     2821 cgcgcatggc ccctacaacc tctcggccag gggcgatctc gtcgaggctg ccgcagcact
     2881 cttcgggctc ctgcacgaga tcgacgccac gggtgtcgcg cgcatcgccg tggcccccgt
     2941 cccgcatcac ggccttggcg aggccatcaa cgaccggctg ctgcgcgcgg cggctccacg
     3001 gggtgcacca tgaagcccag cgctgcaacg ctcgaccgtc tcgccgccgt ggtcggcgaa
     3061 gcgcatgtgc ttcgcgatgc gcctgccatg gacggctaca tgcgcgagtg gcgcggaatc
     3121 tggcatggcg tttcgcccct ggtgctgcgt ccaggctcga cccgcgaagt ctcgcaaatt
     3181 ctcgccatcg caaacgagac gcgcaccgcc atcgtgccgc agtccggcaa caccgggctc
     3241 aacggcggcc agatcccgcg cgacagcggg gatgaagtcc tgctctcgct tgaccgcatg
     3301 acgcagatcc tcgacatcga tgcgatcgac aacaccatca cggtcgaggc gggctgcatc
     3361 ctcaagtctg tgcaggacgc tgccgcagcg gtggaccggc tctttccgct gagcctcgcg
     3421 tccgaaggct cctgccgcat cggcggaaat ctttcgacca atgccggcgg tctcaacgtg
     3481 atcgcctatg gaaatgcccg cgacctctgc ctcggcctcg aggtggtact ggccgatggt
     3541 cgcgtgtgga acggcctcaa acgtctgcgc aaggacaata ccggctacga cctgcgcgac
     3601 ctgttcatcg gtgcagaggg aacgctcgga atcataccgg cagccgtgct gaagcttttc
     3661 ccccgtccgc agcgacacga gacggccttc atcgccgtac ccggccccca ggccgccgtg
     3721 gaactcctgt cgctgctgcg cgcggcgagc ggcaaccgcg tcgtcgcctt cgaactcatc
     3781 gcgtccctgg cgctgcaatt caccgagcgc catgcgggcc tccgctcgcc gctcgagtcg
     3841 gcttcgccgt ggtatgttct tgcggaactg gccgaccccc tgcccggtgc cttcacggcg
     3901 cttctggaag aggccatgga caagggcctt gtcaccgacg ccgccattgc ccagtcggac
     3961 cggcagcgcg acgccttctg gaccatccgc gagaccatct cggattcgca gaagcccgaa
     4021 ggcggttcga tcaagcacga catcgctgtt cccgtctcgc aggtgccgcg cttcctggct
     4081 gcggccgatg cggccgtcgc gcgcttcatg ccgggcgcgc ggctcgtcac cttcggccat
     4141 ctcggtgacg gcaacattca ctacaatgtc tcgcagcccg tcggcatgga tacccagcag
     4201 tatctcgatc agtggaacgc catgaacgac gtggtcttcg aggtcgtcac acgtttcggc
     4261 ggctcgatca gcgccgagca cggcatgggg aggctcaagg cgcaccacat gccgctgatc
     4321 aagagcccgg tggaactcga gatgatgcgc ggcctgaagc gcctcttcga tcccaatacc
     4381 atcctcaatc ccggccgggt gctgcccgaa tgacggcgtt ccgcgagatc gaggacaggg
     4441 atgtagaagc cgtggtcgaa ctatggtcgg cctgcgggct tacgcggccg tggaacgatc
     4501 c
//
LOCUS       DBNGVR010000328         3150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2088086, whole genome shotgun sequence.
ACCESSION   DBNGVR010000328 DBNGVR010000000
VERSION     DBNGVR010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2088086"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..622
                     /locus_tag="AC16A0_06915"
     CDS             41..622
                     /locus_tag="AC16A0_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3105100.1"
                     /translation="MTLPRLFLAAPAIPLELASACLKAACAAGDVASLLVPPGLARDL
                     TPVAQALGVAVLLDGEAREAMYAGCDGVQIDARAEGVDIAGIRSSMAKDRIVGAFAGH
                     SRHFAMEAAEAGADYVALDQKGPTIGGEPIVKWCAAFLNIPCVAFMPCTVQDLDTLLP
                     QKPDFIRPADAMWESESDARRIVSELMQNFRGR"
     gene            619..1515
                     /locus_tag="AC16A0_06920"
     CDS             619..1515
                     /locus_tag="AC16A0_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3105101.1"
                     /translation="MKTLRILLAAWLLSSSAWAAPIDDALSAYKAGNDALALKTAEPL
                     AASGDPRAMKLVGDLYRRGTAVMQNLETAMSWYEKAAKAGNVQATTAMALVYLNGSLG
                     AVNYETGSSLLRQAADAGDAEAQFNMGLLSTGAFGNPPDYTLAADWFRRAADQKHAAA
                     RYQLGLLYLDGRGVEKNLVTAGKLVGEAAQAGDVDAMLDYGVLVMRGEGVKQDIKIGA
                     QWLLLSARRGNVIAQNRVARLYAFGMAVTRDPVEAQKWNILAARGGRNDIELDTLAEK
                     MTEAERAEALARANGFAPEAAP"
     gene            1512..2255
                     /locus_tag="AC16A0_06925"
     CDS             1512..2255
                     /locus_tag="AC16A0_06925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3105102.1"
                     /translation="MSDPVISSPQNPAVKLIRSLAEKKHRQETGLFMAEGEKVLERAR
                     REGWEPETVVSLGPQPHWGKVRQLVADARVMASLSGQKNASPVVAVFRQRWSRDVTPN
                     GTWLALEDLRDPGNLGTIIRTADAVGARGIILAGQSCDPFGPDCVRATMGSIFGVPLI
                     RMTAAELVSTCKSWPGEVAGTHLNGSIDYRRTYGNPTLIVLGSEGKGLSQEVAAACAT
                     LVRIPMKGGPESLNVAIAAGLMLYEAVKP"
     gene            2272..2649
                     /locus_tag="AC16A0_06930"
     CDS             2272..2649
                     /locus_tag="AC16A0_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105103.1"
                     /translation="MFHGMTEYAYLSFVFAGCVAGTLLGIVAHYWRAHATMYAEDLGL
                     REPLNTLSRDNYQFEKHVIGAEWDDAGFWAADSIRNLLYYMASGFIVPLVLGFVLWGE
                     RDLVVTGICQVLTGAGLTPPLCP"
     gene            complement(2650..2970)
                     /locus_tag="AC16A0_06935"
     CDS             complement(2650..2970)
                     /locus_tag="AC16A0_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3105104.1"
                     /translation="MRHVALALALLPVLAAPALATSGPGCLVVVNVAEDDGLNLRSRP
                     SAGSDIVDVLVPGQHGIIHLDAPCEPPHRAWASRWCPVTHYNGDDVTKGWVKARFVRD
                     SDCP"
BASE COUNT          540 a         1042 c         1062 g          506 t
ORIGIN      
        1 ggggcaagtc tgattgaagg ggcgcggagt tcgaatcgac atgaccctgc cacgcctctt
       61 ccttgccgcg cccgccatcc cgctggagct tgccagcgcc tgcctgaagg ccgcctgcgc
      121 ggcgggcgat gtggcaagcc tcctggttcc gcccggcctg gcgcgggatc tcacgcccgt
      181 ggcccaggcc ctcggtgttg ccgttctgct cgacggcgaa gcacgcgagg ccatgtatgc
      241 cggctgcgac ggggttcaga tcgatgcccg cgcggagggc gtcgacattg ccgggatcag
      301 atcgtcaatg gccaaggacc gcatcgtcgg agccttcgcc ggccactcgc gccatttcgc
      361 gatggaagcc gccgaggccg gcgccgacta tgtggcgctc gaccagaagg ggccgacgat
      421 cggcggcgaa cccatcgtga agtggtgcgc ggcgttcctc aacattccct gcgtcgcctt
      481 catgccctgc accgtccagg acctcgacac attgctgccg cagaaaccag actttatccg
      541 ccccgccgat gcgatgtggg agagcgaaag cgacgcccgc cgcatcgtgt ccgaactgat
      601 gcagaatttc aggggccgat gaaaacctta cgtatccttc tcgccgcttg gctgctctcg
      661 tcatccgcat gggccgcgcc gatcgacgac gcgctgtcgg cctacaaggc gggcaacgac
      721 gcgctcgccc tgaagacggc agagccactc gccgcgagcg gcgatccccg cgccatgaag
      781 ctggtgggcg atctctatcg caggggtacg gcggtgatgc agaacctcga gaccgccatg
      841 tcctggtacg agaaggcggc caaggccggc aatgtccaag ccaccacggc catggcattg
      901 gtctatctga acggctcgct gggtgcggtg aactacgaga ccggcagcag cctgctccgg
      961 caggccgcgg atgccggcga tgccgaggcc cagttcaaca tgggactcct cagcacgggc
     1021 gccttcggca atccgccgga ctacacgctg gctgcggact ggttccgccg cgccgccgac
     1081 cagaaacatg ccgccgcccg ctaccagctg ggcctgctct atctcgacgg ccgcggggtg
     1141 gagaagaacc tggtgaccgc cggcaagctg gtgggcgaag ccgcccaggc gggcgacgtc
     1201 gatgccatgc tcgactatgg cgtcctggtg atgcgcggcg agggcgtgaa acaggacatc
     1261 aagatcggcg cgcagtggct tctgctgtcg gcccggcgcg gcaatgtcat cgcgcagaac
     1321 cgggtggcga ggctctatgc cttcggcatg gccgtcacgc gcgatccggt cgaagcgcag
     1381 aagtggaaca tcctggccgc acgcggcggg cgcaacgaca tcgagctcga tacgcttgcc
     1441 gagaagatga ccgaggcgga acgtgccgag gcgctggcgc gtgccaacgg cttcgcgccc
     1501 gaagccgcgc cgtgagcgac ccggtcatca gctcgccgca gaatccggcg gtcaagctga
     1561 tccgttcgct ggccgagaag aagcaccggc aggagaccgg cctctttatg gccgagggcg
     1621 agaaggtgct ggagcgcgcc cgccgcgaag gctgggaacc cgagacggtc gtatcgcttg
     1681 gaccgcagcc gcactggggc aaggtgcggc agctggtcgc cgacgcgcgc gtcatggcca
     1741 gcctgagcgg ccagaagaat gccagtcccg tggtcgccgt gttcaggcag cgctggtccc
     1801 gcgatgtgac gccgaacgga acctggcttg cccttgagga tctgcgcgat cccggtaacc
     1861 tcggaaccat catccgaacc gccgatgcgg tgggcgcacg cggcatcatc ctcgccggcc
     1921 agagctgcga tcccttcggc cccgactgcg tgcgggccac gatgggctcg atcttcggcg
     1981 tgccgctgat ccgcatgacc gcggccgaac tggtttccac ctgcaagtcc tggccgggcg
     2041 aggtcgccgg aacgcatctg aacggcagca tcgattaccg caggacttat ggcaacccga
     2101 cgctgatcgt gctgggcagc gagggcaagg gcctgtcgca ggaggtggcg gcggcctgcg
     2161 cgacgctggt gcgcatcccg atgaagggcg gccccgagtc gctcaatgtg gccattgccg
     2221 cgggtctgat gctctacgag gccgttaagc cctgagtaag cagcctccgg catgttccat
     2281 ggcatgaccg aatacgccta cctcagcttt gtctttgccg gttgcgtggc gggaaccctg
     2341 ctgggcatcg tggcgcatta ctggcgcgcc catgccacca tgtatgccga agacctgggc
     2401 ctgcgcgaac cgctcaatac gctgagccgc gacaactacc agttcgaaaa gcatgtgatc
     2461 ggcgcggaat gggacgatgc gggattctgg gcggccgaca gcatccgcaa tcttctctac
     2521 tacatggcca gcggcttcat cgtgccgctg gtcctgggct tcgtgctgtg gggcgagcgc
     2581 gatctggtcg tcaccgggat ttgccaagtc ctcaccggcg cgggcctcac cccgccgctc
     2641 tgcccgtgat cacggacagt cgctgtcgcg cacgaagcgg gccttgaccc agcccttggt
     2701 gacgtcatcg ccattgtaat gcgtgaccgg acaccagcgg ctggcccagg cgcggtgcgg
     2761 cggttcgcag ggggcgtcga ggtgtatgat gccatgctga ccgggcacca gaacgtcgac
     2821 gatgtccgaa ccggccgagg gccgcgaccg caggttcagc ccgtcgtcct cggcgacatt
     2881 caccaccaca aggcagcccg gaccggacgt ggccagggcc ggagcggcaa ggaccggcag
     2941 cagggcaagg gcaagcgcga catgtctcat gacaacctcc atcaatgatc ccttctcggc
     3001 ccggcaggct gaacccggca tgaatgacgg ggcgtgaacg gcgctcacca cgcgcatggg
     3061 tttttgccct tgtaaacgcc gctggactgg tgaagaggat tgactggaac ttgcccctcc
     3121 gcgggccgcc gggctagctt cggccaatct
//
LOCUS       DBNGVR010000329         1680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3069442, whole genome shotgun sequence.
ACCESSION   DBNGVR010000329 DBNGVR010000000
VERSION     DBNGVR010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3069442"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="AC16A0_06940"
     CDS             <1..1270
                     /locus_tag="AC16A0_06940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR3105105.1"
                     /translation="GLGYVGLPLVNATARAGFRVTGFDIDPAKIDLLNRGRSYTDAVP
                     DFAIDGHRRAGRFHATMDFTEIANCDVVVICVPTPLTRQREPDLSAVRKTAQSIAAAL
                     RKGQLIVLESTTFPGTTREVLVPILEESGLVNRRDFFVGFSPEREDPGNTDFSTSDIP
                     KVVAGDGPEASQTVSAFYAAVFRQIVPVSSPDVAEAVKITENIFRAENIALVNELKTT
                     YDAMGIDIWEVIEAAKTKPFGFMPFYPGPGLGGHCIPIDPFYLTWKAREFDLSTRFVE
                     LAGEINLSMPRYVVDRLAEALDRKQGVGLAGARILILGLAYKKDVGDMRESPSFKLMS
                     LLERRGAAVDYHDPHLPEIGPTREHMEYAGRISVGLDARSVASYHAVLLATDHSNFDY
                     AMIARSAKLIVDTRNGFGRRGLAGENIIKA"
     gene            1300..>1680
                     /locus_tag="AC16A0_06945"
     CDS             1300..>1680
                     /locus_tag="AC16A0_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3105106.1"
                     /translation="MRIAFYAPLKSPEHPTPSGDRQMARLLIRALTAGGATVEAASHL
                     RVYLPAPSQSQWEDCNQRAELEVARIAEAWTSGRVPDLWFCYHPYYKSPDLLGPTLVR
                     RFGIPYVTAEASYSQRRSDGIWREC"
BASE COUNT          319 a          548 c          488 g          325 t
ORIGIN      
        1 cggactgggc tatgtggggt tgcctcttgt caatgcaacc gccagggccg gttttcgggt
       61 gacgggcttc gacattgatc cggcaaagat cgatctcctg aaccgcggac ggtcctacac
      121 cgatgccgtg ccagattttg ccatcgacgg acaccggcgc gcaggtcgat tccatgcaac
      181 catggacttc accgaaatcg ccaactgcga cgtggtcgtg atctgtgtac cgactccgct
      241 caccaggcag cgagaacctg acctctcggc cgtccgcaag accgcgcaga gcattgcggc
      301 cgcactgcgc aaagggcagc tgattgttct tgagtccacc acctttcccg gaacaacgcg
      361 cgaagtcctt gtcccgattc tggaggaaag cggactggtc aatcgccgcg acttcttcgt
      421 cggcttctcg cccgagcgtg aggacccggg caatacggac ttctcgacgt cggacattcc
      481 aaaggtcgtg gctggcgacg ggccggaggc ctcgcagact gtctcggcgt tctatgccgc
      541 cgttttccgc cagatcgtgc cggtctcttc gcccgatgtc gctgaagccg tgaagatcac
      601 cgaaaacatc ttccgcgcgg agaacatcgc cctcgtcaac gagctcaaga ccacctacga
      661 cgccatgggc atcgacatct gggaagtcat cgaggcggca aagaccaagc ccttcggttt
      721 catgcccttc taccccggac ccggactcgg aggccactgc attcccatcg accccttcta
      781 cctgacctgg aaggcgcgcg agttcgatct gtcgacgcgc ttcgtcgagc tggccggaga
      841 gatcaatctg tcgatgccgc gctatgtcgt cgacaggctc gccgaagcgc tcgaccggaa
      901 gcagggtgta ggccttgccg gcgctcgcat cctgatcctt gggcttgcct acaagaagga
      961 tgtgggcgac atgcgggaaa gcccgtcctt caagctgatg tcgctgcttg agaggagagg
     1021 tgccgcagtc gactatcacg accctcatct tcccgagatc ggaccgacac gggaacacat
     1081 ggagtatgcc ggccgcatct cggttggtct ggatgcacgg tcggttgcgt cctaccacgc
     1141 ggtcctgctc gccacggacc acagcaactt cgactacgcc atgatcgcca ggtcggccaa
     1201 gctcatcgtc gacacgcgca atggcttcgg ccgccgcgga cttgctggcg aaaacatcat
     1261 caaggcctga gccatggcat tccgcgccac ggaggcgtca tgcgcatagc cttctatgcc
     1321 cccctgaaat cacccgagca cccaacgccc tccggcgatc ggcagatggc gcggctgctg
     1381 atcagggcgc tgacggccgg tggtgcaacc gtcgaggccg cctcccacct ccgcgtctat
     1441 cttccggcgc cttcgcaaag ccaatgggag gactgcaatc agcgtgccga gctggaggtt
     1501 gcgcgaattg cagaggcatg gacatcaggc cgcgtgcccg atctctggtt ctgctatcac
     1561 ccctattaca agtcgcccga tcttctcgga ccaacattgg tgcgtcgttt cggcattccc
     1621 tatgtcacgg cagaagcatc ctattcacag cggcgcagtg acggcatatg gcgcgaatgt
//
LOCUS       DBNGVR010000330         2101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_913014, whole genome shotgun sequence.
ACCESSION   DBNGVR010000330 DBNGVR010000000
VERSION     DBNGVR010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_913014"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="AC16A0_06950"
     CDS             <1..528
                     /locus_tag="AC16A0_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3105107.1"
                     /translation="EKTAPEALDRATEYFEAIGMQVLRLKREIMGYVCDRLQEALWRE
                     ALHILNKDIATTGDIDDSIVYSAGMRWAFMGSFLTYHVAGGPGGMRDFIKQFDPTLEL
                     DWTDLKFPKWNAALEQRLVEGCEAQAAGRKVSEIEAKRNAILVDMMKLFKKHKIGAGL
                     VLDREMKARKKKSKR"
     gene            556..1326
                     /locus_tag="AC16A0_06955"
     CDS             556..1326
                     /locus_tag="AC16A0_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3105108.1"
                     /translation="MPQQRVMVTAAADGIGRAIAKAFAADGAKVHICDVNEEALAKFR
                     KEFPFIPAAHVDVRNESQLNAWFDAGLKDLGGIDVLVNNAGIKGPTAPVEGVSLADWR
                     ECLEICLDAQFLGAKRVAPLMKAQKSGAIINLSSNAGQHGFGNRTPYAAAKWGVIGLT
                     KSLAIELGPYNVRCNAICPGSVRGDRINRVIEGEAKVRGVPFDVVAAEIVNNQSIARF
                     TEAEEVADLCLYLASPRAAMITGQDIAIDGHVEVFHIK"
     gene            1333..>2101
                     /locus_tag="AC16A0_06960"
     CDS             1333..>2101
                     /locus_tag="AC16A0_06960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3105109.1"
                     /translation="MDFNLTEEQQMIVKTTRDFVVNELYPHEAEIEETGVLRHDLRDA
                     IKTKAIEAGLYAANMPAEVGGAGLDTLTWVLYEKELGRANYALHWTCVARPSNILMAC
                     QGEQREKYLFPSVRGEKSDCLAMTEPGVGSDVRGMKTFARQDGDDFVINGTKHFISHA
                     DEASFVILFAATGEEDTPRGKKKLITAFLIDMGTPGYTVRPGYRNVSHRGYNNCILEF
                     NDCRVHKSQILGQLHKGFEVANDWLGATRLQVAATCIG"
BASE COUNT          430 a          670 c          652 g          349 t
ORIGIN      
        1 gagaagacgg cgcccgaagc gctggaccgc gccacggaat atttcgaagc catcggcatg
       61 caggttctgc gcctcaagcg cgagatcatg ggttatgtct gcgacaggct ccaggaggcc
      121 ctgtggcgcg aggcgttgca catcctcaac aaggacatcg cgaccactgg cgacatcgac
      181 gactcgatcg tctattcggc tggcatgcgc tgggccttca tgggctcctt cctcacctat
      241 cacgtggcgg gcggccccgg cggcatgcgc gacttcatca agcagttcga ccccacgctc
      301 gaactggact ggaccgacct caagttcccg aagtggaacg ccgcgctcga gcagcgcctg
      361 gtcgagggct gcgaggccca ggcggcgggc cgcaaggttt ccgagatcga ggccaagcgc
      421 aacgccatcc tcgtcgacat gatgaagctc ttcaagaagc acaagatcgg cgcgggcctt
      481 gtgctcgacc gcgagatgaa ggcaagaaag aagaagtcca agaggtaagg ccgttcgcgg
      541 ccaggaggag acccaatgcc ccagcaacgc gtgatggtca cggccgccgc agacggtatc
      601 ggccgcgcta tcgccaaggc cttcgcggcc gatggcgcca aggtgcacat ttgcgacgtg
      661 aacgaagagg cgcttgcgaa gttccgcaag gagtttccct tcattccggc ggcgcatgtc
      721 gacgtgcgca acgagtccca actcaacgcc tggttcgacg cggggctgaa ggatctcggt
      781 ggcatcgacg tgctggtcaa caatgccggc atcaaggggc caacagcgcc ggtggagggc
      841 gtgtcgctgg ccgactggcg cgaatgcctg gaaatctgcc ttgacgcgca gttcctcggc
      901 gccaagcggg tggcgccgct gatgaaggcg cagaagtcgg gcgccatcat caatctctcc
      961 tccaatgcgg gccagcatgg ttttggcaac cgcacaccct atgcggcggc caagtggggc
     1021 gtcatcggcc tgaccaaatc gctggccatc gagctcggtc cctacaatgt ccgctgcaac
     1081 gccatctgcc ccggctcggt gcgtggcgac cgcattaaca gggtgatcga gggtgaggcc
     1141 aaggtgcgtg gcgtaccttt cgatgtggtc gccgccgaga tcgtcaacaa ccaatccatc
     1201 gcgcgcttta ccgaagcgga agaagtggcg gacctctgcc tgtacctcgc ctcgccgcgc
     1261 gccgccatga tcaccggaca ggacatcgcc atcgacggcc atgtcgaggt cttccacatc
     1321 aagtagggtc ccatggattt caatctcacc gaagaacagc agatgatcgt gaagacgacg
     1381 cgggatttcg ttgtcaacga gctctatccg catgaagccg agatcgagga aaccggcgtg
     1441 ctgcgccatg atctccgcga cgcgatcaag acgaaggcca tcgaggccgg tctctatgcc
     1501 gccaacatgc cagccgaagt gggcggcgcg ggcctcgata cgctgacctg ggtcctctac
     1561 gagaaggaac tgggccgcgc gaactatgcg ctgcactgga cctgcgtggc acgcccgtcc
     1621 aacatcctga tggcctgcca gggcgaacag cgcgagaagt acctgttccc gtcggtgcgc
     1681 ggcgagaaat cggactgcct cgccatgacc gagcctggtg tcggctccga tgtgcgcggc
     1741 atgaagacct tcgccaggca ggacggcgac gacttcgtta tcaacggcac caagcacttc
     1801 atcagccatg ccgacgaagc gagcttcgtc atcctctttg ccgccaccgg cgaggaagac
     1861 acgccgcgcg gcaagaagaa gctgatcacc gccttcctca tcgacatggg aacgcctggt
     1921 tacaccgtgc gccccggcta caggaacgtc tcgcaccgcg gctacaacaa ctgcatcctc
     1981 gaattcaacg attgccgcgt tcacaagagc cagatccttg ggcagctcca caagggcttc
     2041 gaggttgcga acgactggtt gggcgcaacg cgccttcagg tcgccgcaac ctgcatcggc
     2101 c
//
LOCUS       DBNGVR010000331         2259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_913174, whole genome shotgun sequence.
ACCESSION   DBNGVR010000331 DBNGVR010000000
VERSION     DBNGVR010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_913174"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..238)
                     /locus_tag="AC16A0_06965"
     CDS             complement(83..238)
                     /locus_tag="AC16A0_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HIR3105110.1"
                     /translation="MPRMRRKDELPVKTCAQCGKPFIWRKKWSRDWERVKFCSDRCRM
                     SGKQTRA"
     gene            complement(242..970)
                     /locus_tag="AC16A0_06970"
     CDS             complement(242..970)
                     /locus_tag="AC16A0_06970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3105111.1"
                     /translation="MTLPIIIVGGSGGIGSALAHRLAAAGRALHLIGRDAVKLRATAA
                     ETGASHAAADIGDREALADAIAAAGGAAAGLVYAVGSITLKPLARITDDDVERDFRLN
                     ALGAFRSVQLALPALKANPEVSSVVLFSSIAVTQGFTSHASIGMAKGAVEGLTLSLAA
                     ELAPKLRVNCIAPSLTQTPLAAGLTANAQMASAIAALHPLQRLGEADDVAALAAFLLG
                     PDSGWITGQIIGVDGGRSTLRTKG"
     gene            complement(1002..>2259)
                     /locus_tag="AC16A0_06975"
     CDS             complement(1002..>2259)
                     /locus_tag="AC16A0_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3105112.1"
                     /translation="SILLNTPGESGSIVTALEGNQMARRGRGAAALATAAIGSFVAGT
                     IGTMLLTFLAPLFVKLAILFGPAEYFALMVLAFTTVTTLLGSSVVKGLTSLMIGLAFG
                     LAGTDILTGQPRLVFGIPSMLRGIDTVVVAVGLFAVGEALYMASRRDLREAVILPIKG
                     SLFMTREEWRRSWKPWLRGTALGFPFGALPAGGTEIPTFLSYFMEKKLSKNPEEFGKG
                     AIEGVAGPEAANNAAVAGVLVPLLALGLPTSATAAILLAAFEQYNLQPGPQLFTASGP
                     LVWGLIASLYIGNVMLLVLNLPLAGLWVRLLAIPQPLLYGGILVFSTLGVYSLSNSTF
                     DLVLLFVVGLIGFVMRRHDFPVAPAIIGLILGPMAEAQFRRALTISQGDASVFFTHPI
                     SLALLVIAALVLLLPVLVRGLPVQKL"
BASE COUNT          457 a          711 c          748 g          343 t
ORIGIN      
        1 acgatgtcgc gtgacggatc gatgtccgcg agactggata tgtcgcgggt cagatgatcg
       61 ccgagaacga cgcgaagggt actcatgcgc gggtctgttt gcccgacatg cggcaacggt
      121 cggagcagaa cttgacccgc tcccagtccc tggaccactt cttgcgccag atgaaaggct
      181 tgccgcactg cgcgcaggtc ttcaccggta gctcgtcctt gcgacgcatg cgcggcatgg
      241 ctcagccctt ggtgcgcagt gtcgaacggc caccgtcgac gccgatgatc tgccccgtga
      301 tccaaccgga gtcgggaccc agcaggaagg cggcaagtgc ggcgacgtca tccgcctcgc
      361 ccaggcgctg caggggatgc agtgcggcaa tggccgaagc catttgcgca ttggcggtca
      421 ggccggccgc cagtggcgtc tgggtgagag acggcgcgat gcagttcacc cgcagtttcg
      481 gcgcaagctc ggccgcaagc gacagggtca gcccctcgac cgctcccttg gccattccga
      541 tcgacgcgtg cgaggtgaag ccctgggtca cagcgatgct cgagaacagc accacggagg
      601 aaacttccgg gttggccttg agcgcaggca atgccaactg cacggaacgg aacgcgccga
      661 gggcattgag gcgaaagtcg cgctcgacgt cgtcatcggt gatgcgggcc agtggcttga
      721 gcgtgatcga gcccaccgca taaaccaagc ccgccgcggc gccacctgct gcagcgatcg
      781 cgtcggctag cgcctcgcgg tcgccgatgt ctgccgcggc atggcttgct ccggtttcag
      841 cggccgtggc ccgcagcttc accgcgtcgc gtccgatcag gtgaagcgcc cggcctgccg
      901 cagcgagtcg gtgggccagg gcgctcccga tgcccccgct gccgccgaca atgataatgg
      961 gtaaggtcat ctgttgaata cggaaaccgc tgccgagcgg ttcagagctt ctgaacgggc
     1021 agcccgcgaa cgagtaccgg cagcagcagc accagcgctg caatgacaag aagcgcgagc
     1081 gagatcggat gggtgaagaa gaccgaggca tcgccctggc tgatggtcag cgcgcggcgg
     1141 aactgtgctt ccgccatggg cccgaggatc agcccgatga tggctggtgc cacagggaaa
     1201 tcatgacggc gcatcacgaa cccgatcagg cccaccacaa acagcaggac aagatcgaag
     1261 gtcgaattgc tcaggctata gacacccagc gtcgagaaaa cgagaatacc gccatagagc
     1321 aagggctggg ggatggccag caaccggacc cagagaccgg ccaatggcag gttcagcacc
     1381 agcagcatca cgttcccgat gtaaaggctg gcgatcagac cccagaccag cggtccggag
     1441 gcggtgaaga gctgcggccc aggctgcaga ttgtactgtt cgaaggccgc cagcaggatt
     1501 gccgctgtgg cggaggtggg caagcccagc gccagcagcg gcaccagcac gccggccacc
     1561 gcggcattgt tggcggcttc cggtccggca acgccttcga tcgcaccctt gccgaattcc
     1621 tctggattct tggagagctt cttttccatg aagtaggaca ggaaggtcgg gatttcggtc
     1681 cccccggccg gaagcgcgcc gaagggaaag cccagtgccg ttccgcgcag ccagggcttc
     1741 caggagcggc gccattcctc gcgcgtcatg aacagcgaac ccttgatcgg caggatcact
     1801 gcctcccgca ggtctctccg cgatgccatg tagagagctt cacctaccgc gaagaggcca
     1861 acggccacga cgacggtgtc gatgccgcgc agcatgctgg gaatgccaaa aaccaggcgc
     1921 ggctgccccg tcaggatatc ggtaccggca agaccgaagg ccagcccgat catcaggctg
     1981 gtcaaaccct tgaccaccga actgccgagc agcgtggtga cggtggtgaa ggccagcacc
     2041 atgagcgcga agtattcggc cggaccgaag agaatggcga gcttcacgaa gagcggcgcc
     2101 aggaaggtca gcagcatggt accgatggta cctgcaacga aggatccgat tgccgccgtg
     2161 gcgagcgccg ccgcaccacg tccgcgccgc gccatctggt taccttccag ggcggtgacg
     2221 atggatccgg attcgcccgg cgtgttgaga aggatcgat
//
LOCUS       DBNGVR010000332         6287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3069763, whole genome shotgun sequence.
ACCESSION   DBNGVR010000332 DBNGVR010000000
VERSION     DBNGVR010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3069763"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /gene="phnY"
                     /locus_tag="AC16A0_06980"
     CDS             complement(<1..1332)
                     /gene="phnY"
                     /locus_tag="AC16A0_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HIR3105113.1"
                     /translation="MNMTTAKRPVIREAMRIGGRKVTTDDVVPVHYPYTNEVIGTVPA
                     GRAEHAAEAFAIAAAYKPRLTRYERQQILFRAAELIRGRRDQLSDLITLELGLSKKDS
                     LYEVGRAYDVYSLAGQLAILDDGQIFSCDLTPQGKPRKIFTKREPVGVISAITPFNHP
                     LNMVSHKVAPAIATNNCVVCKPTELTPLTAIALADILYESGLPPEMFQVVTGWPDQIG
                     DEMVTNDKISVITFTGGVRVGKLIAAKAGYKRAALELGGNDPLIVLDDLSDSDLDKAA
                     ELAVAGSTRNSGQRCTAVKRILVQERIADRFVELVLAKARTIKFGDPMDPETDLGTVV
                     HEQAARTFEARVYKAAEQGAKVLYDPGRKGAVLPPITVDFVPHTSELVFEENFGPVIP
                     VVRVPNDDAEVIRISNSTPFGLSSGVCTNSLPRITRFIEGLEVGTVNIWEVP"
     gene            complement(1334..2578)
                     /gene="phnA"
                     /locus_tag="AC16A0_06985"
     CDS             complement(1334..2578)
                     /gene="phnA"
                     /locus_tag="AC16A0_06985"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HIR3105114.1"
                     /translation="MTMKTVSVNGRDYHWPRAPLVVICCDGSEPDYMEVAMTAGLMPN
                     LKRIIAKGENLRGHSAIPSFTNPNNLSIVTGRPPAVHGICGNYLIDPATGQETMMNDP
                     KWLRAPTIFAAFQKAGARVAVVTAKDKLRLLLGKGLVFDGTAVAFSSEKADKATREDN
                     GIENPCALVGMAVPDVYSAELSEFVFAAGVKLLASMRPDLMYLSTTDYVQHKYAPGSD
                     GANRFYAMMDRYLGELDAGGATIVIVADHGMKDKHLADGAPDVIYLQDLLDARFGKGK
                     TKVILPITDPYVVHHGALGSFATVYAYGPAISDVMAWIARQPGIDVVLDKTEGCARFE
                     LPEDRMGDVIVISGGPAGSKVIGTSRDKHDLSGLNEPLRSHGGLTEQEIPVISNRKLK
                     NLPAQLRNFDAFAIGCNNIVES"
     gene            complement(2575..3735)
                     /locus_tag="AC16A0_06990"
     CDS             complement(2575..3735)
                     /locus_tag="AC16A0_06990"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HIR3105115.1"
                     /translation="MDRASTRYNGPEGQEDMPYLLTPGPLTTSRSVKATMLADWGSRD
                     VEFRKVVAEIRKGLLAMAGCDASYECVIMQGSGTFAIEAALGCFCPAGAGKTLVVANG
                     AYGDRAAKILERIGRHVSKIDKGDSAAPTVEEVAATLDADAAITHVWMIHCETTSGII
                     NPIQDMAKMVKDKKRVFMVDAMSSFGALPLDMMDGLDVMVSSSNKCIEGVPGFSYVLV
                     KRDMLEASKGRCHSVVLDLHEQWKGLESNGQVRFTPPTHALVAFHQAMREHAEEGGVA
                     ARGERYRRNAQQLIKGMRDMGFTTLLSDNQAGPIIQTFLTPRDPNFDFEHFYEALRSR
                     GFAIYPGKLTRRPSFRIGTLGKVDDRVMAGVLKAIREVLAEMKVTDLSPLEA"
     gene            3826..4716
                     /locus_tag="AC16A0_06995"
     CDS             3826..4716
                     /locus_tag="AC16A0_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3105116.1"
                     /translation="MRHAQLKAFHAVAIHGGFSRAAERLSLTQPAVSDHVRKLEESYG
                     VQLFNRGSSGVALTAMGRSLFAIAERLFEAETEARDLLSRAQTLEEGQLTIGADAAVH
                     ILPRVARFRAAFPGIAVRLVTGNSAELLARLEAFAIDVAIVADRPSDERFVVRRLRED
                     RLVAVVPGQGDKAVRITLDALMNRPMILREEGSVTRRLLTDELRRRGLKPAGIIEIEG
                     REAVREAVAQGLGVAIMSEGELSIDSRLRSLAISDWAAVMSEWLIGLKSRAGLHVIRS
                     FLDMPEASGQAAPAKARLRR"
     gene            4713..5531
                     /locus_tag="AC16A0_07000"
     CDS             4713..5531
                     /locus_tag="AC16A0_07000"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3105117.1"
                     /translation="MSFRAACIQNSATPDVAHDIAVLARLIRDAAAQGAGFVATPEYC
                     AGLDTRDGLLYPVAPEEKDHPVLPAMAALAGELGIWLLIGSIGVRASDGRINNRSYML
                     RPDGGIAARYDKIHMFDVDLGPGKVYRESATIAPGDTAVIAPCAEGSIGLSICYDLRF
                     AALYRTYAQAGAEMLATPAAFTRMTGEAHWHVLQRARAIENGAYVIAPCQYGTLAGGS
                     QCYGHSLIVDPWGRVLADGGTEEGIILAEIDLAMVAQTRSRIPSLRHDRPFALG"
     gene            5598..6155
                     /locus_tag="AC16A0_07005"
     CDS             5598..6155
                     /locus_tag="AC16A0_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3105118.1"
                     /translation="MFGYGILALIAAVIGYLIYAYNALVKLKNLVSEGWSGIDVQLKR
                     RADLIPNLIETVKGYAAHEDKLFRDIAELRAKSIAGGTVAEQSAVGQAMSAALGRLFA
                     IAEAYPELKADANFRDLQEKLAGIEDEIQLSRRYYNGAVRNLNTMIESFPSNFVANYF
                     KFEKSEFFEIGDPAEREVPKVDFKK"
BASE COUNT         1046 a         1984 c         2102 g         1155 t
ORIGIN      
        1 gggcacttcc cagatgttca cggtgccaac ctcgaggcct tcgatgaaac gtgttatgcg
       61 cggcaggctg ttggtgcaga cgccggagga gagaccaaaa ggcgtggagt tggaaatgcg
      121 gatcacctcg gcatcgtcgt tgggcacgcg cacgacgggg atgaccggcc cgaagttctc
      181 ttcgaagacc agctccgagg tatgcggcac gaagtcgacg gtgatgggcg gcaggacggc
      241 gcccttgcgg cccggatcgt agagcacctt ggcgccctgc tctgccgcct tgtagacgcg
      301 cgcctcgaaa gtcctggcgg cctgctcgtg aaccacggtg ccgaggtcgg tttccggatc
      361 catgggatcg ccgaacttga tcgtcctggc cttggccagg accagctcga cgaagcggtc
      421 ggcgatgcgc tcctgcacca ggatgcgctt gaccgccgtg cagcgctggc cggagtttcg
      481 cgtcgaaccg gccactgcca gttcggctgc cttgtcgaga tcgctatcgg agagatcgtc
      541 cagcacgatc agcggatcgt tgccgccgag ttcaagtgct gcgcgcttgt agcccgcctt
      601 ggccgcgatg agcttgccga cgcgcacgcc gccggtgaag gtgatgacgc tgatcttgtc
      661 gttggtcacc atctcgtcgc cgatctgatc gggccagccg gtcaccacct ggaacatctc
      721 gggcggcagg ccggattcgt agaggatgtc ggccagcgcg atggcggtga gcggcgtcag
      781 ttcggttggc ttgcagacca cgcagttgtt ggtggcgatc gccggggcca ccttgtgcga
      841 gaccatgttg agcggatggt tgaagggcgt gatcgccgag atcacgccca ccggctcgcg
      901 cttggtgaag atcttgcgcg gcttgccctg cggcgtgaga tcgcaggaga agatctggcc
      961 gtcgtcgaga atggccaatt gtccggccag ggaatagacg tcataggcgc ggcccacttc
     1021 gtagagcgag tccttcttgg acaggcccag ttccagcgtg atgaggtcgg agagctggtc
     1081 gcggcggccg cggatcagct cggcggcgcg gaacaggatc tgctggcgct cgtagcgcgt
     1141 gagcctgggc ttgtaggccg ccgcgatggc gaaggcctcg gccgcgtgct cggcgcggcc
     1201 ggcgggcacc gtgccaatca cctcgttggt gtagggatag tgcaccggca ccacgtcgtc
     1261 ggtcgtcacc ttgcggccgc cgatgcgcat ggcttcccgg atcaccgggc gcttggccgt
     1321 ggtcatgttc atctcagctc tccacgatgt tgttgcagcc gatggcgaag gcatcgaagt
     1381 tgcgcagctg cgcgggcaga ttcttcagct tgcggttgct gatcaccggg atctcctgct
     1441 cggtcagtcc gccatgcgat cgcagcggct cgttcagccc cgagagatcg tgcttgtcgc
     1501 gcgaggtgcc gatcaccttg gagccggcgg ggccacccga gatcacgatg acgtcgccca
     1561 tgcgatcctc gggcagttcg aaacgggcac agccttcggt cttgtcgagc accacgtcga
     1621 tgccgggctg gcgggcgatc caggccatga catcggaaat cgcgggccca taggcgtaga
     1681 ccgtggcgaa ggacccaagc gcgccgtgat gcaccacata gggatcggtg atcggcagga
     1741 tcaccttggt cttgcccttg ccgaaccgtg cgtcgagcag gtcctgcaga tagatcacgt
     1801 cgggcgcgcc gtcggccagg tgcttgtcct tcatgccgtg gtcggcaacg atgacgatgg
     1861 tggcgccacc ggcgtcgagt tcgccgagat agcggtccat catggcatag aagcggttgg
     1921 caccgtccga ccccggcgca tacttgtgtt gcacatagtc ggtggtcgac agatacatga
     1981 ggtcaggtcg catgctggcc agcagcttca cgccggccgc gaagacaaac tcggagagtt
     2041 cggccgagta gacatccggc acggccatgc cgaccagtgc gcaagggttc tcgatgccgt
     2101 tgtcctcgcg tgtcgccttg tcggccttct ccgaggaaaa cgccaccgcc gtgccgtcga
     2161 agacgaggcc cttgccgagc agcaagcgca gcttgtcctt ggccgtcaca accgccactc
     2221 tggcgcccgc cttctggaag gccgcgaaga tggtgggcgc gcgcagccac ttcggatcgt
     2281 tcatcatcgt ctcctggccg gtggccggat cgatgagata gttgccgcag atgccgtgca
     2341 cggcgggcgg gcgccccgtg acgatcgaga gattgttggg attggtgaag ctgggaatgg
     2401 ccgaatggcc gcgcaggttc tcgcccttgg cgatgatgcg cttgaggttc ggcatgaggc
     2461 cggccgtcat ggccacctcc atgtagtcgg gttcgctgcc gtcgcagcag atcacgacga
     2521 gcggcgccct aggccagtgg tagtcgcggc cattcacgga gacggtcttc atcgtcatgc
     2581 ctcgagcggg gaaagatcgg tcaccttcat ctcggccagc acctcgcgga tggccttgag
     2641 cacgccggcc atcacccggt cgtcgacctt gccgagggtg ccgatgcgga agctgggccg
     2701 ccgtgtcagc ttgccggggt agatcgcgaa gccgcggctg cgcagcgctt cgtagaaatg
     2761 ttcgaagtcg aagttgggat cgcgcggggt cagaaaggtc tggatgatcg ggccagcctg
     2821 gttgtccgag agcagggtgg tgaagcccat gtcgcgcatg cccttgatca gctgctgggc
     2881 attgcggcga tagcgctccc cgcgggcggc aacgccgccc tcctcggcat gttcgcgcat
     2941 ggcctggtga aaggcgacca gcgcgtgggt gggcggcgtg aagcggacct ggccgttgga
     3001 ctcaagcccc ttccactgtt cgtggagatc cagcaccacc gaatggcagc ggcccttgct
     3061 ggcctccagc atgtcgcgct tgaccagaac gtaggagaag ccgggcacgc cctcgatgca
     3121 cttgttcgat gacgagacca tcacgtcgag gccgtccatc atgtcgagcg gcagcgcacc
     3181 gaaggacgac atggcgtcga ccatgaagac gcgcttcttg tccttgacca tcttggccat
     3241 gtcctggatc ggattgatga tgcccgaggt ggtctcgcag tggatcatcc agacatgggt
     3301 gatggcggca tcggcgtcga gggtggcggc cacctcctcc actgtcgggg cggcgctgtc
     3361 gcccttgtcg atcttgctga cgtgacggcc gatgcgttcg aggatctttg cggcacggtc
     3421 gccataggcg ccgttggcaa ccaccagcgt cttgccggcg ccagccggac agaagcagcc
     3481 gagggccgcc tcgatggcga aggtgccgga gccctgcatg atcacgcact cataggaggc
     3541 atcgcagcct gccatggcga ggaggccctt gcggatttcc gccacgacct tgcggaactc
     3601 gacatcgcgc gatccccagt cggccagcat ggtggccttc acgctgcgcg aggtggtgag
     3661 cggcccgggg gtgagcagat agggcatgtc ctcctggcct tcggggccat tgtaacgggt
     3721 gctggcgcgg tccatcggta actcctgtgt gcgggccgat gcatggggac ttgacggcta
     3781 tcagtcaaat cgtatgttct tatatagatc ataagttcta cagatatgcg tcacgctcag
     3841 ctcaaggcct ttcacgcggt cgccatccat ggcgggttct cgcgcgcggc ggagcgcctt
     3901 tcgctgacac agcccgcggt ctccgaccat gtccgcaagc tcgaggaaag ctatggagtc
     3961 cagcttttca accgcgggtc atccggagtc gcgctgaccg ccatgggccg cagcctcttt
     4021 gccattgccg aacggctctt cgaggccgag acagaagcgc gggacctgct gtcgcgggcg
     4081 cagacccttg aagaaggcca gctcaccatc ggggccgatg cggccgtcca catcctgccg
     4141 cgggttgcgc gtttccgcgc ggcattcccc gggatcgcgg tgcgcctggt caccggcaac
     4201 tcggccgagc tgctggcccg gctggaagcc tttgcgatcg acgtggcgat cgtggccgac
     4261 cgtccctccg acgagcgctt cgtggttcgc cgcctgcgcg aggaccggct ggtggccgtg
     4321 gtgccggggc agggcgacaa ggccgtgcgc ataacgctcg atgccctcat gaaccggccg
     4381 atgatcctgc gcgaggaagg gtcggtcacg cgccggctgc tgaccgacga actgcgccgc
     4441 cgcggcctga agcccgccgg catcatcgag atcgagggcc gcgaggcagt gcgagaggcg
     4501 gtggcccagg gcctgggagt cgccatcatg tcggaaggcg agctttccat cgactcgcgg
     4561 ctgcgcagcc ttgccatatc ggactgggcg gcggtcatgt cggaatggct catcggcctg
     4621 aaatcccgcg ccggcctgca tgtcatccgc tccttcctcg acatgccgga agcgtctggc
     4681 caagctgcac cagccaaggc tagactgcgg cgatgagctt tcgcgccgcc tgtatccaga
     4741 attccgccac gcccgatgtg gcgcatgaca tcgcggtcct cgcccggctg atccgcgacg
     4801 ctgcggcaca gggcgcaggc ttcgtcgcca cgcccgagta ttgcgccggc ctcgatacgc
     4861 gcgacgggct gctctatccg gtggcgcccg aagagaagga ccatccggtg ctgcccgcca
     4921 tggccgcgct tgccggcgaa ctcggcatct ggctgctgat tggctcgatc ggagttcgtg
     4981 catccgacgg tcgcatcaac aaccgctcct acatgctgag gcccgacggc ggcatcgccg
     5041 cgcgctacga caagatccac atgttcgacg tcgatctcgg gccgggcaag gtctatcgcg
     5101 aatcggccac tatcgcgccg ggcgacacgg cggtgatcgc gccctgcgcc gaaggcagca
     5161 tcggtctttc gatctgctat gacctgcgct ttgccgcgct ctaccgcacc tatgcgcagg
     5221 ctggcgcgga gatgcttgcg acgccggcag ccttcacccg catgacgggc gaggcgcatt
     5281 ggcatgtgct gcagcgggcg cgtgccatcg agaacggtgc ttatgtgata gcgccctgcc
     5341 agtatggcac gctggcgggt ggcagccagt gctacggtca ttcgctgatt gtcgatccct
     5401 ggggccgcgt gctggccgat ggcggaaccg aagaaggcat catccttgcc gagatcgatc
     5461 tggcgatggt cgcgcagaca cgcagccgca tcccgtcgct gcgccacgac aggccctttg
     5521 cgcttggctg acaacatccg cttgattgcg tcatccgctg ccatgataat cgccgcgtca
     5581 acaggggagt gactcgcatg ttcggctacg gaatcctggc gctgatcgcg gcggtgatcg
     5641 gttacctcat ctacgcctac aatgcgctgg tgaagctcaa gaacctcgtc tccgagggct
     5701 ggagcggcat cgatgtgcag ttgaagcgcc gcgccgatct cattcccaac ctgatcgaga
     5761 cggtgaaggg atatgccgct cacgaggaca agctgttccg cgacatcgcc gaactgcgcg
     5821 ccaagagcat cgccggcggc acggtggccg aacagtcggc cgtgggccag gccatgagcg
     5881 cggccctcgg caggctcttt gccatcgccg aggcctatcc ggaactgaag gccgatgcca
     5941 atttccgcga tctgcaggag aagctcgccg gcatcgagga cgagatccag ctgtcgcggc
     6001 gctactacaa cggcgctgtg cgcaacctca acaccatgat cgagtccttc ccctccaact
     6061 tcgtcgccaa ctacttcaag ttcgagaagt ccgagttctt cgagatcggc gatcccgcag
     6121 agcgcgaagt tccgaaggtc gacttcaaga agtaaacgcc atgcgcgccc tgatgctggc
     6181 cttctgcctc ctcctcggct tcgccgccga ggccctggcc gaagagaaga tcacctcctt
     6241 catcagcgac gtcacggtga atgccgatgc gtcgctcgag gtgcgcg
//
LOCUS       DBNGVR010000333         2863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2284822, whole genome shotgun sequence.
ACCESSION   DBNGVR010000333 DBNGVR010000000
VERSION     DBNGVR010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2284822"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /gene="thrS"
                     /locus_tag="AC16A0_07010"
     CDS             <1..1503
                     /gene="thrS"
                     /locus_tag="AC16A0_07010"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3105119.1"
                     /translation="ELFKVELVDAIPGNQDIKFYDQGGWTDLCRGPHMTSTGKIGTAF
                     KLMKVAGAYWRGDSKNPMLTRIYGTAWASDKDLQSYLTALEEAEKRDHRKLGREMDLF
                     HFQEEGPGVVFWHAKGWTMFQQLIAYMRRRLAKLDYQEVNAPQLLDKSLWETSGHWGW
                     YREAMFAATSAGDTTDDDQVFAIKPMNCPGHVQIFKHGLRSYRELPLRLAEFGNVHRY
                     EPSGALHGLMRVRGFTQDDAHIFCTEEQMADECLKINDLILSTYRDFGFEEIVVKLST
                     RPEKRVGSDAAWDRAEDIMSKVLVQIAEESGGKIKTAVNPGEGAFYGPKFEYVLRDAI
                     GRDWQCGTTQVDFNLPERFGAFYIGADGEKKVPVMIHRAICGSMERYLGILIEHFAGH
                     FPLWLAPLQVVVATITSDGDAYALEVRDALRRAGLRADADLRNEKINYKVREHSVQKV
                     PVILVVGKREMDERTVNVRRLGSQNQSSMSLDAAISALAAEAAPPDVQRS"
     gene            1627..2589
                     /locus_tag="AC16A0_07015"
     CDS             1627..2589
                     /locus_tag="AC16A0_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3105120.1"
                     /translation="MIRRHLLAAALAVAVGTAGFSGTAFAVNDLRIIAPAGPGGGWDQ
                     TARALADVMAASGATGITVENVPGAGGTVGLAQLVDQEKGKPNVMMVNGLVMVGAILT
                     NQSAVNLGMTTPVARLTGEYEVIVVPASSEVKTLADLVAKLKADTGTVAFGGGSAGGT
                     DHILAGLVAKAAGGDVTKLNYVPFSGGGEALAAVMGGHVVAGISGYSEWAGQIASGEL
                     RALAISAPERQAGIDIPTLKEQGVEVELANWRAIVAPPGLDEAQTKALRDAVDAAVKS
                     DAWKKVLAEKKWTDLYLPGDDFGKLLEAENARVTEVLKTVGLVQ"
     gene            2623..>2863
                     /locus_tag="AC16A0_07020"
     CDS             2623..>2863
                     /locus_tag="AC16A0_07020"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3105121.1"
                     /translation="MAGLLIGLGLIAIAAVIAVDVSGMRVPPVHAKVGPRFFPLLVSC
                     GLAVAGAVTAWQSWRGTFDSEDMPPTDWAAVAIVSA"
BASE COUNT          532 a          843 c          962 g          526 t
ORIGIN      
        1 gaattgttca aggtcgagct ggtcgatgcc attccgggca atcaggacat caagttctat
       61 gaccagggcg gctggaccga cttgtgccgc gggccgcaca tgacgtcgac cggcaagatc
      121 ggcaccgcct tcaagctcat gaaggtggcg ggcgcttatt ggcggggcga ttcgaagaat
      181 ccgatgctga cgcgcatcta cgggacggcc tgggccagcg acaaggatct gcagtcctat
      241 ctcacggcgc tcgaagaggc cgagaagcgc gaccatcgca agctgggccg cgaaatggac
      301 ctgttccatt tccaggaaga aggcccgggc gttgtcttct ggcacgccaa gggctggacc
      361 atgttccagc agctgattgc ctacatgcgc cgccggctgg cgaagcttga ctaccaggag
      421 gtgaatgcgc cgcaacttct cgacaagtcg ctctgggaaa cctccgggca ctggggctgg
      481 tacagggagg cgatgtttgc ggcgacctct gccggcgaca cgacggacga cgaccaggtc
      541 ttcgccatca agccgatgaa ctgccccggg catgtgcaga tcttcaagca tggcttgcgg
      601 tcctatcgcg aactgccgct ccggcttgcc gagttcggca atgtgcaccg ctacgaaccg
      661 tcgggcgccc tgcacgggct gatgcgcgtg cgcggcttca cgcaggacga cgcgcacatc
      721 ttctgcacgg aagagcagat ggccgacgag tgcctcaaga tcaacgatct catcctctcc
      781 acctacaggg atttcggctt cgaggagatc gtggtgaagc tgtcgacgcg tccggaaaag
      841 cgcgtgggtt cagatgccgc ctgggatcgg gccgaggaca tcatgtcaaa ggtgctcgtc
      901 cagatcgccg aagagtccgg cggcaagatc aagacggcgg tgaacccggg cgaaggcgct
      961 ttttatgggc cgaagttcga gtatgttctg cgcgacgcca tcggccgcga ctggcaatgc
     1021 ggaacaacgc aggtggattt caatctgccg gaacgcttcg gcgccttcta tatcggcgcc
     1081 gacggcgaga aaaaggtgcc ggtgatgatt catcgcgcca tctgtggatc catggagcgc
     1141 tatctgggca ttctcatcga gcactttgcc ggtcacttcc cgctctggct tgctcccttg
     1201 caggttgtcg tggcgaccat cacgtccgat ggcgacgcct atgcgctcga ggtgcgcgat
     1261 gcgctgcgtc gcgcggggct gagggccgat gccgatctgc gcaatgaaaa gatcaactac
     1321 aaggtccgcg aacattcggt tcagaaggtt ccggtcattc ttgtggtcgg caagcgcgag
     1381 atggacgagc gcacagtgaa cgtccgcagg cttggctcgc agaaccagtc cagcatgagc
     1441 ctcgatgcag cgatctcggc cctggccgcg gaagcagcac cgccggatgt acagcggtcc
     1501 tgatcgtgac gaagatcgtt gcgcggggca ggatgctggg ttagcgtccg gctgagtgga
     1561 ggcaattgag aagagccagc atggctgcgg ctgccgcaac gggacatcga gagggaggac
     1621 ccaaacatga ttagacgtca tcttcttgca gcagccttgg ccgtcgccgt gggtactgcc
     1681 ggattctccg gcactgcgtt tgcggtgaac gatttgagga tcatcgcccc ggccggtccg
     1741 ggcggcggat gggatcaaac ggcgcgtgcg ctggcggacg tcatggcagc ctcaggtgca
     1801 actggcatca ccgtcgagaa tgtcccggga gcgggtggca cggttggtct cgcgcagctc
     1861 gtggaccagg aaaagggcaa gccgaacgtc atgatggtca acggcctggt catggtcggc
     1921 gccatcctca cgaaccagtc tgcagtcaat ctgggtatga ccacgccggt tgcccgcctg
     1981 accggcgagt atgaggtcat cgtcgtacct gcctcatccg aggtgaagac tctggctgac
     2041 ctggtggcga agctcaaggc tgataccgga accgttgcct ttggcggcgg ctctgctggc
     2101 ggcaccgacc acattctcgc gggcctggtg gccaaggccg ccggcggcga tgtgacgaag
     2161 ctcaactacg tgcccttctc gggcggcggc gaagccctcg cggccgtcat gggcggacat
     2221 gtcgtggccg gcatcagtgg ctatagcgag tgggccggcc agatcgcgtc gggcgaactg
     2281 cgtgcgctgg cgatctcggc gcccgagcgc caggcgggta tcgacattcc cacgctgaaa
     2341 gagcagggcg ttgaagtcga gcttgccaac tggcgcgcca tcgtggcacc tcccggcctg
     2401 gacgaagccc agacaaaggc tctgcgcgat gcggtggatg ccgccgtcaa gagcgatgcg
     2461 tggaagaagg tattggctga gaagaagtgg accgatctct atctgcccgg cgacgacttc
     2521 ggcaagctgc ttgaggccga gaatgcgcgg gtcaccgaag tcctgaagac ggtcggtctc
     2581 gtccagtaac gcacagaggt gagcacgcaa cgcaagacgt ccatggccgg tcttctgatc
     2641 ggccttggac tcattgcgat tgcggccgtg attgccgtag atgtgtccgg catgcgcgta
     2701 ccaccggtcc acgccaaggt cggtccgcgc ttcttcccct tgctggtgtc ctgcgggctt
     2761 gccgttgccg gcgccgttac ggcatggcag tcgtggcgcg gaaccttcga ttccgaggat
     2821 atgccgccga ccgactgggc ggcggtcgcg atcgtcagcg cgg
//
LOCUS       DBNGVR010000334         3720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_516744, whole genome shotgun sequence.
ACCESSION   DBNGVR010000334 DBNGVR010000000
VERSION     DBNGVR010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_516744"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="AC16A0_07025"
     CDS             complement(<1..674)
                     /locus_tag="AC16A0_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlgB N-terminal domain-containing protein"
                     /protein_id="HIR3105122.1"
                     /translation="MAEPSWKIDDAEVEALVAGHHDDPFRRLGLHREGRQWVVRTVVP
                     GAEGVEAIDADGKSVGRLDRRHAAGFFEGPVKIKDRQALRYLAANGQGTWTVLDAYLF
                     GPVLGPLDDYYMAEGNHLRLYDKLGAHPIRHEGHDGVHFAVWAPNASRVSVVGDFNHW
                     DGRRHVMRKRMDIGVWEIFVPGATIGQGYKFELLDSGGRLLPLKSDPFGFHAEIRPKT
                     ASRVAET"
     gene            complement(688..3132)
                     /locus_tag="AC16A0_07030"
     CDS             complement(688..3132)
                     /locus_tag="AC16A0_07030"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR3105123.1"
                     /translation="MIKPAPRGTDAETIRAEILEKLAYSIGKDPAVAKRHDWLNATVL
                     ALRDRIIDRWMESTKRAYQTKAKRVYYLSLEFLIGRLLRDAMSNLQLTGAFQQALQAL
                     NVDLDLIAALEPDAALGNGGLGRLAACFMESMASLDIPAYGYGIRYNHGLFRQKIVDG
                     WQVELPEDWLENGNPWEFPRREAAYEIGFGGHVTLDGEQGNSQKRRWHPGERLVASAC
                     DTPIVGWRGKRVNTLRLWTARAVDPIRLDAFNSGDYAAALGESNKAEIITRVLYPSDS
                     TPAGQELRLRQEYFFTAASLQDIIRRHVQQYDEVRSLADKVAIQLNDTHPAVAVAELM
                     RILIDNHGLGWDEAWEITKGCIGYTNHTLLPEALESWPVHLFEHVLPRHMQIVYGINA
                     KLIAEAQVKPGCTPDFLSSISLIDEHHGRRLRMGQLAFVGSHSINGVSALHTSLMKES
                     VFRDLNKLYPGRINNKTNGITPRRWLLGVNPALAELLSEAAGKGVLDDAMLLSKAKPF
                     AKDPAFRERFAAIKRRNKEELSNLVMARMGIRLDPAAMFDVQVKRIHEYKRQLMNILE
                     TIALYNTMRAHPDRPWAPRVKIFAGKAAASYAQAKLIIKLANDVASVVNHDPAIRDIL
                     KVVFIPNYNVSLAEVIVPAADLSEQISTAGMEASGTGNMKFALNGALTIGTLDGANVE
                     ILEHVGRDNIFIFGLTADEVEARRREGYNAAATIDAQPDLKGVLDALSSGVFSPDDHG
                     RFRPIVDALYHGDWFMVAADFNAYTKAQRGVDLLWHRTKDWTEKAILNTVNMGWFSSD
                     RTIRDYARDIWNVPTA"
     gene            complement(3284..>3720)
                     /locus_tag="AC16A0_07035"
     CDS             complement(3284..>3720)
                     /locus_tag="AC16A0_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3105124.1"
                     /translation="RSKRDAFAAAMDAQYARLDDFLTWQAPERTFLFDAFGLAEAVFT
                     PLFARFWFLDYYEKHEIPEHLARERRWRDACLAHPAAQQVSKEEIVKLYFDYALGMGN
                     GSLPAGRSRSSFVFEPHWRERPWPPRDKWAVAASDAELGLEK"
BASE COUNT          671 a         1136 c         1203 g          710 t
ORIGIN      
        1 gtctcggcca cgcgggacgc cgtcttcggc cggatttcgg cgtggaaccc gaaaggatcg
       61 ctcttcagcg gaagcagccg cccgcccgaa tccagcagtt cgaacttgta gccctgcccg
      121 atggtggcac ctgggacgaa gatttcccag acgccgatgt ccatgcgctt gcgcatgaca
      181 tggcggcggc cgtcccagtg attgaagtca ccgacaacgg agacgcgcga cgcattgggc
      241 gcccagaccg cgaaatgcac gccgtcgtgg ccttcgtgac ggatggggtg cgcaccgagc
      301 ttgtcgtaga ggcggaggtg attgccctcg gccatgtagt agtcgtcgag cgggcccagc
      361 acagggccga agagataggc gtcgagcacc gtccaggtcc cctgcccgtt ggcggccagg
      421 tagcgcaggg cctgacggtc cttgatcttg accgggcctt cgaagaaacc tgcggcgtgg
      481 cgccgatcga ggcggccgac ggacttgccg tcggcgtcga tggcctcgac gccttccgcg
      541 ccgggaacga ccgtccgcac gacccactgc cggccttcgc ggtgaaggcc gagccgcctg
      601 aagggatcat catgatggcc tgcgaccagc gcctcaacct cggcgtcatc gatcttccag
      661 ctgggttcgg ccatacggtc cttctcctca ggctgtggga acgttccaga tgtcgcgggc
      721 atagtcgcgg atcgtccggt cggacgagaa ccatcccata ttcaccgtat tgaggatggc
      781 cttttccgtc cagtccttcg ttctgtgcca cagcagatcg acgccgcgct gtgcctttgt
      841 ataggcgttg aagtcggccg ccaccatgaa ccaatcgcca tggtaaagtg catcgacgat
      901 ggggcggaac cggccatggt cgtcgggcga gaaaacgccc gacgacaggg cgtcgagaac
      961 gcccttgaga tcgggctggg catcgatggt agccgccgca ttgtagccct cccgccgcct
     1021 ggcttcgacc tcgtcggcag tcagtccgaa gatgaagata ttgtcccggc ccacatgctc
     1081 aaggatctcg acattggcac catccagcgt tccgatggtc agcgcaccat tgagagcgaa
     1141 cttcatgttg ccggtgccgg aggcttccat gccggccgtc gagatctgct ccgacagatc
     1201 tgctgccggg acgatcactt ccgccaggct gacattatag ttcgggatga agacgacctt
     1261 caggatatcg cggatggccg gatcatgatt cacaacgctg gccacgtcat tggcgagctt
     1321 gatgatgagt ttggcctggg catagctggc ggcggccttg ccggcgaaga tcttgacgcg
     1381 cggcgcccag ggccggtcgg gatgcgcccg catggtgttg tagagggcaa tggtctcgag
     1441 gatgttcatg agctggcgct tgtactcatg gatgcgcttg acctgaacgt cgaacatggc
     1501 tgcgggatcg aggcggatgc ccatgcgggc catgacgaga ttcgagagtt cctccttgtt
     1561 gcggcgcttg atggcagcga agcgctcgcg gaatgccgga tctttggcaa agggcttggc
     1621 cttggagagc aacatcgcgt catcgaggac gcccttgccg gctgcttcgc tcaggagctc
     1681 ggccagtgcc ggattcacgc ccagcagcca gcgccgcggc gtgatgccgt tggtcttgtt
     1741 gttgatgcgc cccggataga gcttgttgag atcgcggaac acgctttcct tcatcagcga
     1801 cgtatgaagt gccgacacgc cgttgatgct gtgcgatccg acaaaggcca gctgacccat
     1861 gcgcaggcga cggccatgat gttcgtcgat gagcgagatg gaggacagga aatccggcgt
     1921 gcagccgggt ttgacctgcg cctccgcaat cagcttcgca ttgatgccgt agacgatctg
     1981 catgtggcgc ggcaggacat gctcgaagag atgcaccggc cagctttcca gggcctcggg
     2041 caacagcgtg tgattggtat agccgatgca acccttggtg atttcccagg cctcgtccca
     2101 gccaagtcca tgattgtcga tgaggatgcg catcagctcg gccactgcca ccgccggatg
     2161 cgtgtcattg agctgaattg ccaccttgtc ggccagcgag cgcacctcgt catactgctg
     2221 gacatggcgg cggatgatgt cctgcagcga ggccgcggtg aagaagtact cctgacggag
     2281 acgaagttcc tgtcccgccg gcgtcgagtc cgacggatag aggacgcggg tgatgatctc
     2341 ggccttgttg ctctcgccga gcgctgcggc atagtcaccg ctgttaaagg catcgagccg
     2401 gattgggtcc actgcacgcg ccgtccacag gcgcagcgtg ttcacccgct tgccgcgcca
     2461 gccgacgatc ggcgtgtcgc aggccgaggc gaccagccgt tcccccggat gccagcggcg
     2521 cttctggctg ttgccctgct caccgtcgag ggtgacgtga ccgccgaagc cgatctcata
     2581 ggcagcctcg cggcgcggga attcccaggg gttgccgttc tccagccaat cctccggcag
     2641 ttccacttgc cagccgtcga caatcttctg acggaaaaga ccatgattgt agcggatgcc
     2701 atagccatag gcgggaatat cgaggctggc catgctctcc atgaagcagg cggcgaggcg
     2761 gccgagaccg ccattgccaa gcgctgcatc gggctcaagg gcagcgatca gatcgaggtc
     2821 cacgttcagc gcctgaaggg cctgctggaa ggcccccgtc agctgcaggt tgctcatggc
     2881 atcgcgcagc aggcgcccga tgaggaactc cagcgacagg tagtagacgc gctttgcctt
     2941 ggtctgatag gcgcgcttgg tagattccat ccagcggtcg atgatgcggt cgcgcagcgc
     3001 caggaccgtc gcgttgagcc agtcatggcg cttggcgacg gccgggtcct tgccgatcga
     3061 gtaggcgagt ttttcaagga tctcagcgcg gatggtctcg gcatcggttc cgcgcggtgc
     3121 aggcttgatc atcgttcctc atctcttctc tggcttcggt gggaccgggc gccgcttata
     3181 ggagatgccc gcaacccggc gcaacggcta gccataataa atcaactcgc gtgatttatt
     3241 caagcagaat ctgaggtacg cacctaaagt gccgggattg ccgtcacttc tcaagcccga
     3301 gttcggcgtc tgatgccgcg actgcccatt tgtcgcgggg cggccatgga cgctcgcgcc
     3361 aatgcggttc gaagacgaag ctcgaccggc tgcgacccgc tggcaggctg ccgttcccca
     3421 tgcccagggc atagtcgaag tagagtttca cgatctcctc cttgctgacc tgctgggcag
     3481 ccggatgggc gaggcaggca tcccgccagc gccgctcacg ggcgagatgc tcgggaatct
     3541 catgcttctc gtaatagtcg aggaaccaga agcgcgcgaa gagcggcgtg aacaccgctt
     3601 cggccagccc gaaggcgtcg aagagaaagg tgcgctcggg cgcctgccac gtcaggaaat
     3661 cgtcaagtcg cgcatactgc gcatccatgg ccgccgcgaa tgcgtcccgc ttgctgcggt
//
LOCUS       DBNGVR010000335         4083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_516959, whole genome shotgun sequence.
ACCESSION   DBNGVR010000335 DBNGVR010000000
VERSION     DBNGVR010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_516959"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="AC16A0_07040"
     CDS             complement(<1..1280)
                     /locus_tag="AC16A0_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR3105125.1"
                     /translation="MIAELGHFALMLATGVAVFQFLVPLYGAQRGDRMLMRSAAPASL
                     LQVFLIGISFIALTHAYVVSDFSVLNVAENSHSAKPLIYKISGVWGNHEGSMLLWVLI
                     LAVFGAAVAVFGDNLPLELKSRVLAVQGSVGLAFLLFLVLTSNPFARLDPPPIEGNGL
                     NPILQDPALAFHPPFLYAGYVGLSIAFSFAVAALIEGRVDAAWARWVRPWTLAAWMFL
                     TVGIAMGSWWAYYELGWGGWWFWDPVENASFMPWLVATALIHSAIVVEKRNALKIWTV
                     LLSILAFSLSLVGTFLVRSGVLTSVHAFAVDPERGTFILLILMIFIGGALALFAWRAQ
                     TLKAGGLFAPVSREGGLVANNLLLCVSCLAVFVGTLYPLALETWNGAKISVGPPYFNL
                     TFGPIILPLLMLVPIGPFLAWKRGDLGQAMRRLR"
     gene            complement(1277..1723)
                     /gene="ccmE"
                     /locus_tag="AC16A0_07045"
     CDS             complement(1277..1723)
                     /gene="ccmE"
                     /locus_tag="AC16A0_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR3105126.1"
                     /translation="MTRKQKRLSLILGGLAILGLAAGLVLYALRDTIVFFHTPSEITE
                     KKVPAGQRIRLGGMVEEGSIRKESGTVTSFVVTDTLATLKVTYDGILPDLFREGQGVV
                     AEGVVQPDGIFVADTVLAKHDENYMPKELADSLKEKGVKLGKGAEQ"
     gene            complement(1720..2817)
                     /gene="ccmI"
                     /locus_tag="AC16A0_07050"
     CDS             complement(1720..2817)
                     /gene="ccmI"
                     /locus_tag="AC16A0_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR3105127.1"
                     /translation="MIWLFCAALTLLVLALLLWPVLRGERDGDAADRSAYDRTVFRDQ
                     LAELERDLSRGLIGEKEAEAARNEISRRLIAAAEQGGGTHGRRLPLAIAMATVLIVPA
                     VAVPLYLKVGNPALPDVPLQARIDRAAETGDFDALIAKVERHLAGAPGDVQGWQVLAP
                     AYQRVERWSDAANAYQNIMRLTKPDAELLSNYAEMLVFAANGMVSAEAHKAVRDALAL
                     DPKMPKARFFDGLALKQEGKTAEAKASFEAFLADSPADAPWRPMLEAELRDLGARPPA
                     LSQEQMDQAAGMSSEDQQAMIRSMVDGLDERLKANGNDLDGWLRLIRARSVLNEAEKA
                     SAAYKQARTQFKDNPEALAALDGLAQELNIQ"
     gene            2978..>4083
                     /locus_tag="AC16A0_07055"
     CDS             2978..>4083
                     /locus_tag="AC16A0_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020812334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HIR3105128.1"
                     /translation="MKPRYSIFSLARNAMNYHKDWERAWRSPTPKAEYDAVIVGAGGH
                     GLATAYYLAKNHKMTNIAVVEKGWLGGGNTGRNTTIIRSNYLQDPSIAIYELARSLYE
                     GLSQELNYNMMFSPRGVLMLCQTEHEFRAFKRTAHANRLAGLDCRMVTPAEVKEIVPI
                     INDDPNCRYPILGAYYQPRGGTGRHDAVAWGYARGADSMGVDIIQNCEVTGILRDGGK
                     VTGLETTRGTIRTKKIGVVTAGHVSTIMDMAGVRMPIESQCLQALVSEPIKPVIDCVV
                     MANTVHGYISQSDKGELVIGGGTDPYNNYSQRGSFPALEHTVTALVETFPIISRLRML
                     RLWGGIVDMTGDRSPIISKTPVDGLYVNCGWGTGG"
BASE COUNT          782 a         1332 c         1313 g          656 t
ORIGIN      
        1 atgcgcaggc gccgcatcgc ctgccccagg tcgccgcgtt tccaggccag gaaaggcccg
       61 atcggcacca gcatgagcaa gggcaggatg atggggccga aggtgaggtt gaaatagggc
      121 gggccgactg agatcttggc tccgttccag gtctcgagcg cgagcggata aagcgtaccg
      181 acgaagaccg cgaggcagga cacgcagagc agcaggttgt tggccaccag cccgccttcg
      241 cggctcaccg gcgcgaaaag gccgccggcc ttgagcgtct gggcgcgcca cgcaaagagc
      301 gccagcgcac cgccgatgaa gatcatcagg atgaggagga tgaaggtgcc gcgttccgga
      361 tcgacggcaa aggcgtgaac ggaggtcagc acgcccgagc gcaccaggaa tgtgccaacc
      421 agtgaaagcg aaaaggcgag gatcgacagc agtaccgtcc agatcttcag cgcattgcgc
      481 ttttccacca cgatcgccga atggatcagc gcggtggcga ccagccaggg catgaagctg
      541 gcattctcca ccgggtccca gaaccaccag ccgccccagc ccaactcata gtaggcccac
      601 caggaaccca tcgcgatacc aacggtgagg aacatccatg ccgccagcgt ccagggcctg
      661 acccagcgcg cccaggccgc gtcgacgcgg ccttcgatca gggcggcgac ggcgaaggag
      721 aaggcgatgg agaggcccac atagccggcg tagaggaagg gcggatggaa ggcgagcgct
      781 ggatcctgca ggatgggatt gaggccgttg ccctcgatgg gcggcggatc gagccgtgca
      841 aagggattcg aagtcagcac caggaagagc aggaaggcga gacccaccga tccctgcacg
      901 gcaaggacgc gcgacttgag ttccagcggc aggttgtcgc cgaagaccgc gacggcggcg
      961 ccgaagacgg cgaggatcag aacccacaga agcatcgagc cctcgtggtt cccccacacg
     1021 ccggaaatct tgtagatcag aggctttgcc gaatgcgagt tctcggccac gttgagcacc
     1081 gagaagtccg agacgacata ggcgtgggtc agggcgatga aggagatgcc gatcagaaag
     1141 acctgaagca atgacgccgg cgctgcgctg cgcatcagca tgcggtcgcc gcgctgggcg
     1201 ccatagagcg gaaccagaaa ctggaacacc gccacgccgg tggccagcat cagcgcgaaa
     1261 tggcccagtt cggcgatcat tgctctgctc ccttgcccag tttcacgccc ttttccttca
     1321 ggctgtcggc cagttccttc ggcatgtagt tctcgtcatg cttggccagc accgtatcgg
     1381 caacgaagat gccgtcgggc tgcaccacgc cttcggccac cacgccctga ccctcgcgga
     1441 agagatcggg cagaataccg tcataggtga ccttcagcgt ggccagcgta tcggtgacga
     1501 cgaaacttgt cactgtgccg ctttccttcc tgatgctgcc ttcctcgacc atgccgccga
     1561 ggcggatacg ctggccggcc ggcaccttct tctcggtgat ctcgcttggg gtgtggaaga
     1621 acacgatggt atcgcggagc gcatagagaa ccagcccggc ggcaaggccg agaatggcga
     1681 ggccgccgag gatgagtgac aggcgcttct gcttgcgggt cattggatgt tcagctcctg
     1741 cgccaggccg tcgagtgcgg caagcgcctc gggattgtcc ttgaactgcg tccgtgcctg
     1801 cttgtacgcg gcggaggcct tttccgcctc gttcagcacg ctccgtgcgc ggatgagacg
     1861 cagccagccg tccagatcat tgccattggc cttgagacgc tcgtccagcc cgtcgaccat
     1921 cgagcggatc atcgcctgct ggtcttcgct gctcatgccg gcggcctgat ccatctgttc
     1981 ctggctcagc gccggcggcc tggcgcccag gtcgcgcagc tcggcctcca gcatgggccg
     2041 ccagggcgca tcggcgggcg aatcggccag gaaggcctcg aaggacgcct tggcctccgc
     2101 cgtcttgccc tcctgtttca gcgcgaggcc atcgaagaac cgcgccttgg gcatcttggg
     2161 atcgagcgcc agcgcatcgc gcaccgcctt gtgggcctcg gccgagacca tgccattggc
     2221 cgcgaagacc agcatctcgg catagttgga caggagttcg gcgtcaggct tggtgagcct
     2281 catgatgttc tgataggcgt tggcggcgtc cgaccagcgt tcgactcgct gataggcggg
     2341 cgccagaacc tgccagccct gcacgtctcc gggagcgccg gcgagatgcc gttccacctt
     2401 ggcaatgagg gcatcgaaat cgccggtttc ggccgccctg tcgatgcggg cctgcagcgg
     2461 cacgtcggga agggccgggt ttccgacctt gagatagagc ggcacggcca ccgccggcac
     2521 gatcagcacc gtcgccatgg cgatcgccag gggcaagcgg cggccatgcg ttccgccgcc
     2581 ctgctccgcc gccgcgatca agcgccgcga aatctcgttg cgcgcggctt cggcttcctt
     2641 ctcgccgatc agaccgcgcg acaggtcgcg ctcgagctcg gccagctggt cgcgaaacac
     2701 ggtgcggtca taggcgctgc ggtcggcggc atcgccatcc ctctcgcccc gcagcaccgg
     2761 ccacaggagc aaggccagaa ccaaaagcgt cagcgctgcg cagaaaagcc atatcatgat
     2821 gagctatgag ttattccggg acatggcata gatgtaattc accttgcgga tcaagtttct
     2881 ggtcgcattg tcgcatggca gcatttgggc gtcggaaatc ccttggttgg ccatgccccc
     2941 atttgttaga tagcggtcaa ctgcaaggaa tgcacccatg aagccgcgct attccatctt
     3001 ttcgctcgcg cgcaacgcga tgaactacca caaggattgg gagcgcgcct ggcgttcgcc
     3061 cacgccgaag gccgagtatg acgcggtgat cgtgggcgcc ggcgggcacg ggctggccac
     3121 ggcctactac ctcgccaaga accacaagat gaccaacatc gccgtggtcg agaagggctg
     3181 gctcggcggc ggtaacaccg ggcgcaacac caccatcatc cgctccaact acctgcagga
     3241 cccgtccatc gccatctacg agctcgcccg ctcgctctac gaaggactgt cgcaggagct
     3301 gaactacaac atgatgttca gcccgcgcgg cgtcctgatg ctgtgccaga ccgaacacga
     3361 gttccgcgcc ttcaagcgca cggcgcacgc caaccgcctc gccggcctcg actgccgcat
     3421 ggtcacgccg gcggaggtca aggagatcgt ccccatcatc aacgacgatc ccaactgccg
     3481 ctatcccatc ctcggcgcct attaccagcc gcgcggcggc acggggcgtc atgacgcggt
     3541 ggcctggggc tacgcgcgcg gcgcagattc catgggcgtc gacatcatcc agaactgcga
     3601 ggtcaccggc atcctgcgcg acggcggcaa ggtcacgggg ctcgagacca cgcgcggcac
     3661 gatccggacg aagaagatcg gcgtcgtcac cgcgggccat gtctccacca tcatggacat
     3721 ggcgggcgtg cgcatgccga tcgagagcca gtgcctgcag gcgctggtct ccgagccgat
     3781 taagccggtg atcgactgcg tcgtcatggc caatacggtg catggctaca tcagccagtc
     3841 cgacaagggc gagctggtga tcggcggcgg caccgatccc tacaacaact attcgcagcg
     3901 cggctccttc ccggcgctcg aacataccgt gacggcgctg gtcgagacct tcccgatcat
     3961 ctcgcgcctg cgcatgctcc ggctctgggg cggcatcgtc gacatgaccg gagaccgctc
     4021 gccgatcata tccaagacgc cggtcgacgg gctctacgtg aactgcggct ggggcaccgg
     4081 cgg
//
LOCUS       DBNGVR010000336         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3660653, whole genome shotgun sequence.
ACCESSION   DBNGVR010000336 DBNGVR010000000
VERSION     DBNGVR010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3660653"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="AC16A0_07060"
     CDS             <1..1191
                     /locus_tag="AC16A0_07060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3105129.1"
                     /translation="DPTSLRPRDVGELMVLAKKFWDLGPGEMAQTLRFWTMSISDFLD
                     EYFENKVIKAYQAVASIIGTALGPMSPGTAYVLLHHAMGDVDGNVGAWGFARGGMGSI
                     TKALTASFKAAGGEVRTGSGVDRILVKNGRAVGVVLANGDEVHGKRVVSNMDVKRTFL
                     KHVDERELPGDFVKRVKNFKIRGSSGKLNIALDRAPKFTALPEGAPNIKGDMHFTDSI
                     EKMERAYDDWKAGHFSRDPFQDMMIPTMIDPTMTPPGKHFMSCFVQYAPPRIEGRDWS
                     DADRDAFGKTCIDQIEAYAPGFKDSILHVEIRTPRELEAEVGLTEGNIFQGELTFDQL
                     LFNRPVPGYAQYRSPIKDLWICGSSTHPGGGVMGAPGRNAAAEILRELKVTATAMSDA
                     YAVL"
     gene            1178..>2609
                     /locus_tag="AC16A0_07065"
     CDS             1178..>2609
                     /locus_tag="AC16A0_07065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3105130.1"
                     /translation="MPSFDTIVIGGGTNGLACAAKLQSSGRKVLVLEARPEAGGAACG
                     SEFAPGYRSPGLAHLLYLLDPRVEKGMALAQHGFAYAGPALTSTAVSATGDHLVLDKS
                     YGATITGSLAAADRDAWSRLRAQLLGFANILAPFKTITPPRIAKSAGNDYLKLAKLGL
                     AARMLGKDQFRELLRMLLINVHDVLNDELTDARLKGLVAFDTTLGSWLGPRSPNSLIL
                     LLNRLAGQAAGEAAAVAYPRGGMGALSAAMGKAAAACGVTVRTDARAAGLIIENDRVA
                     GVTLAGGEEIRAATVVSAISPKTTFLQLAGPRHLDAGFVKRIRDQKSRGAAAKLHLAL
                     KGKPDFRGADLASRLVIAPSEDAVERAFNPVKYNEVPARPVMEVIIPSAHEQGYAPDG
                     HHVLSAIVQYAPHAPESGHERARADMLENTLAVLEDHAPGLRSMTASAEMLMPYDIEA
                     RFGMVGGNWHHGELAVEQMLFLRPLPG"
BASE COUNT          440 a          906 c          822 g          441 t
ORIGIN      
        1 gacccgacca gcttgcgtcc gcgcgacgtc ggcgagctga tggtccttgc caagaagttc
       61 tgggacctcg gcccgggcga gatggcgcag acgctgcgct tctggacgat gtcgatctcc
      121 gacttcctcg acgaatactt cgagaacaag gtgatcaagg cctaccaggc cgtcgcttcc
      181 atcatcggaa cggcgctcgg ccccatgtcg cccggcaccg cctacgtgct tctgcatcat
      241 gccatgggcg acgtcgacgg caatgtcggc gcctggggtt tcgcccgcgg cggcatgggc
      301 tcgatcacca aggcgctcac cgcctccttc aaggcggcgg gtggcgaggt ccgcaccggc
      361 agcggcgtcg accggatcct ggtgaagaac ggccgcgcgg tcggcgtggt cctggcgaat
      421 ggcgacgagg tgcatggcaa gcgcgtcgtg tccaacatgg acgtgaagcg caccttcctg
      481 aagcatgtcg acgagaggga gcttccgggc gacttcgtga agcgcgtgaa gaacttcaag
      541 atccgcggct cttccggcaa gctcaacatc gcgctcgacc gcgcgccgaa attcacggcc
      601 ctgcccgagg gcgcgcccaa catcaagggc gacatgcact tcaccgactc gatcgagaag
      661 atggagcgcg cctatgacga ctggaaggcc ggccatttca gccgcgatcc gttccaggac
      721 atgatgatcc cgaccatgat cgatcccacc atgacgccgc ctggcaagca cttcatgtcc
      781 tgcttcgtgc agtatgcgcc gcccaggatc gagggacgcg actggagcga cgccgaccgc
      841 gacgccttcg gcaagacctg catcgatcag atcgaagcct atgcgcctgg cttcaaggac
      901 tcgattctgc atgtcgagat ccgcacgccg cgcgagctcg aagcggaagt gggactgacc
      961 gaaggcaaca tcttccaggg cgagctcacc ttcgaccagc tgctcttcaa ccggcctgtt
     1021 cctggctatg cgcagtaccg aagccccatc aaggatctct ggatctgtgg gtcgtccacc
     1081 caccccggcg gcggcgtcat gggcgccccc ggccgtaacg ccgccgccga aatcctccgt
     1141 gaactgaagg ttaccgcgac agccatgagt gatgcctatg ccgtcctttg atacgatcgt
     1201 catcggcggc ggcaccaacg gtctggcctg tgctgcgaag ctgcagtcgt ccggccgcaa
     1261 ggtgctggtg ctggaagctc ggcccgaggc cggcggtgcg gcatgcggga gcgaattcgc
     1321 gcccggctac cgcagcccgg gccttgccca ccttctctat ctccttgacc cgcgcgtcga
     1381 gaagggcatg gccctggcgc agcacggctt tgcctatgcc ggcccggcac tgacctcgac
     1441 ggccgtgtcg gccacgggcg atcacctcgt tctggacaag tcctatggcg cgacgatcac
     1501 cggcagtctt gccgccgccg atcgcgacgc ctggagcagg cttcgcgccc agcttcttgg
     1561 tttcgccaac atcctggcgc ccttcaagac catcacgccg ccgcgcatcg ccaagtccgc
     1621 cggcaacgac tacctcaagc tcgcgaaact cggccttgcg gcccgcatgc tgggcaagga
     1681 ccagttccgc gagttgctgc gcatgctgct gatcaatgtg catgacgtgc tcaacgacga
     1741 gctgaccgat gcccggctga aaggcctcgt cgccttcgac actacgctcg ggtcctggct
     1801 cggtccgcgc tctcccaatt ccctgatcct gctgctcaac cgcctggccg gtcaggccgc
     1861 gggcgaggcg gccgcggtcg cctacccgcg tggtggcatg ggcgctctgt cagccgcgat
     1921 gggcaaggcg gccgcggcat gcggcgtcac tgtccgcacc gatgcccgcg cggcggggct
     1981 catcatcgag aacgacaggg tggcaggcgt cacgcttgcc ggcggcgagg agatccgcgc
     2041 cgccaccgtg gtctcggcca tcagtcccaa gaccaccttc ctccagcttg ccgggccgcg
     2101 ccacctcgac gccggcttcg tcaagcgcat ccgcgatcag aagtcgcgcg gtgccgccgc
     2161 caagctgcat cttgcgctca agggcaagcc ggatttccgc ggtgccgatc tcgcctcgcg
     2221 cctggtcatt gcaccctccg aagatgcggt ggaacgcgcc ttcaatccgg taaagtacaa
     2281 tgaagtgccg gcgcgtcccg tgatggaggt gatcattccg tccgcgcatg agcagggtta
     2341 cgctcccgac ggccaccatg tgctgtcggc catcgtgcag tacgcgcccc atgcgccgga
     2401 gtcgggtcac gaaagggccc gcgccgacat gctcgagaac acgctcgccg tcctggagga
     2461 tcatgccccc ggcctgcgca gcatgaccgc gagcgccgag atgctgatgc cctatgacat
     2521 cgaggcccgc ttcggcatgg tcggcggcaa ctggcatcat ggcgaactcg cggtggagca
     2581 gatgctcttc ctgcgcccgc tgcccggga
//
LOCUS       DBNGVR010000337         6351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4249202, whole genome shotgun sequence.
ACCESSION   DBNGVR010000337 DBNGVR010000000
VERSION     DBNGVR010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4249202"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="moaA"
                     /locus_tag="AC16A0_07070"
     CDS             complement(<1..955)
                     /gene="moaA"
                     /locus_tag="AC16A0_07070"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3105131.1"
                     /translation="MSQATMIDPFGRHVTYLRVSVTDRCDFRCVYCMAEDMTFLPKRD
                     LLSLEELDRVCTAFVEKGVKKIRLTGGEPLVRKGIMTLVASLGRHLRSGAMEELTLTT
                     NGSQLAKHAAELAVHGVRRINVSVDTLDDAKFKAITRWGELARVRDGIRAAQAAGLAI
                     KINAVALKGVNDAEIPEMIRWAHGEGMDLTLIETMPLGDIDGDRTDQYLPLSVVRAGL
                     MDRFTLEDIPYKTGGPARYVRVKETGGRLGFITPLTHNFCESCNRVRLTCTGTLYMCL
                     GQEDAADLRAPLRASEGNDLLNAAIDEAIGRKPKGHDFVIDR"
     gene            complement(999..1892)
                     /gene="gcvA"
                     /locus_tag="AC16A0_07075"
     CDS             complement(999..1892)
                     /gene="gcvA"
                     /locus_tag="AC16A0_07075"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HIR3105132.1"
                     /translation="MPKLPPLNALKCLEAAARSQSFSRAAEELHVTQSAVSHQIRLLE
                     DWFGMPLFERRGRQTVPTPKGEELAKSLAEAFDIIGLACKRIAQSDAGPALTIAALPS
                     IATIWLIPRLTRFFSERPEISVKVVYAFHDQALDFDDIDIAILWGTGDWEGCRATRFL
                     DGATVAVCNRALLDREGPLDSPQTLLGRPLLHDTNRHDWQTYMRRAGLKHAGPAPGPI
                     FQDFNLLRAAALAGQGIALCPRLLIADDLDSGRLVQVSEVVIKEDHAYHVIEPADPGH
                     RLAAAIAAFKSWLLSVPAPGG"
     gene            1990..3276
                     /locus_tag="AC16A0_07080"
     CDS             1990..3276
                     /locus_tag="AC16A0_07080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR3105133.1"
                     /translation="MEDTVPALERPSLVDYRNNGKRPKPTFSAAEMQRRQDNIRGWMA
                     RSNVDACLFTSYHNICYLSDFLYCYFGRRYGLVITQKGATSVSAAIDGGQPARRTFGD
                     NVTYTDWSKENYFTAVQGLVKGARRIGIEFDHVNLDFRKQLQDAFPGVDFVDVAAPSM
                     RMRMIKSAEEIKHITKMTRIADIGGAAVVEATAVGVPEHEVALHSTQAMVREIARVFP
                     NAELMDTWTWFQSGVNTDGAHNPVTSRKIRRGDILSLNCFPMVAGYYVALERTLFAET
                     ASKEHLRLWEVNCHVHDEGKKLLVPGAKCSDVAKALNDIYASHDLLKYRTFGYGHSFG
                     VLCHYYGREGGLELREDCDTVLEPGMVVSMEPMIMIPEGQKGAGGYREHDILVITEKG
                     NRNITGFPYGPEHLIVQPHRAARRAGPVKSRASRKK"
     gene            3383..4501
                     /locus_tag="AC16A0_07085"
     CDS             3383..4501
                     /locus_tag="AC16A0_07085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105134.1"
                     /translation="MARDIATDDMMVRFVDVQKSYDGETLVVKNLNLDIAKGEFLTML
                     GPSGSGKTTTLMMLAGFEPATHGEIYVKGQPINNVPPHKRGIGMVFQNYALFPHMTIN
                     ENLAFPLQVRGVGKAETEERVAKILKMVELPQMGNRRPGQLSGGQQQRIAVARALVFE
                     PDLVLMDEPLGALDKQLREQMQYEIKHIHERLGVTIVYVTHDQSEALTMSNRIAVFND
                     GVVQQLSTPDELYERPVNSFVAQFIGENNKLKGTVSEIGADKVATVKLESGDIVKALA
                     VNVKGKGDRTLLSVRPERIEINPTKKSADVILDGRIAELIYLGDHIRARLEVAGHDDF
                     IVKVPNKGGAATVAEGREVKVGWRSEDCRALDYMDFRN"
     gene            4515..5231
                     /locus_tag="AC16A0_07090"
     CDS             4515..5231
                     /locus_tag="AC16A0_07090"
                     /inference="COORDINATES: protein motif:HMM:NF018420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HIR3105135.1"
                     /translation="MANRRAGVILTRLIASVLGLVLIFVPPPPGRAQDYEVDLALVLA
                     IDCSFSVDSEEYRLQMEGLGRAFLREEVKAAIRQGVRQRIAVTAVQWSDENNQRVILP
                     WVIIEGNADADEIGMRLASSPRVLAQGGTSISTALLFSAKLFDTAPSAERRVIDVSGD
                     GRNNIGPPTAAARDRVVAQGITINGLSILNEWDTLNLYFESHVVGGPGHFVIPAADYE
                     DFAEAIFRKLLREITGPGIS"
     gene            5310..>6351
                     /locus_tag="AC16A0_07095"
     CDS             5310..>6351
                     /locus_tag="AC16A0_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3105136.1"
                     /translation="MQPFTLSSIPEIHFGPGRLADLGGKAVTLAGQGGHVLVVADPAL
                     AALGITARAVESLAQAGFATSVYDALKGEPKQSDIDSAANMAREAKSSLIVGLGGGSA
                     LDTAKLVACCAVSGQSAAAYALCATPLPAQPLPIIAVPTTAGTGSETTRVSVFANAER
                     QKVWAWGEELKPDLAILDPELTIGVPPHITAATGLDALVHAIEACTNKHRTDGNDLYC
                     LQAITLISANLERAIGNPGDIAARGAMLLGSCYAGIGIDNCGTALAHNISHAIADLAP
                     IPHGRATGLAMLATIDWVAEGHPAAFARVARAMSSVDAVSAYDRLVRATGIKISLEGD
                     GLDLTNPELLARH"
BASE COUNT         1186 a         2011 c         2058 g         1096 t
ORIGIN      
        1 gccggtcgat gacgaagtca tggcccttgg gcttgcggcc gatcgcctcg tcgatggcag
       61 cgttgagcag gtcgttgccc tcggaggccc gcaggggcgc gcgcaggtcg gcggcgtctt
      121 cctggccaag gcacatgtag agcgttccgg tgcaggtcag ccgcacgcgg ttgcagcttt
      181 cgcagaaatt gtgggtgagc ggcgtgatga agccgaggcg tccgccggtc tctttcaccc
      241 gcacgtagcg cgccggtccg ccggtcttgt agggaatgtc ctccagcgtg aaccggtcca
      301 tgaggccggc ccggacgacc gacaggggca gatactgatc cgtgcggtcg ccatcgatgt
      361 cgcccaaagg catggtctcg atcagcgtca ggtccatgcc ttcgccatgt gcccagcgga
      421 tcatctcggg aatctccgcg tcattgacgc ccttgagcgc caccgcgttg atcttgatgg
      481 caaggcccgc ggcctgcgcg gcgcggatcc cgtccctcac cctggccagt tcgccccagc
      541 gggtgatggc cttgaacttc gcgtcgtcca gggtgtcgac cgaaacgttg atgcggcgca
      601 cgccatggac ggccagttcg gcagcatgct tggccagctg gctgccgttg gtggtgagcg
      661 tcagctcctc catcgcgccg gagcggagat ggcggccgag gcttgcaacc agtgtcatga
      721 tgcccttgcg caccagcggt tcgccgccgg tgaggcggat cttcttgacg cccttctcga
      781 cgaaggccgt gcagacccga tcgagttcct cgagcgagag caggtcccgc ttgggcagga
      841 aggtcatgtc ctcggccatg caatagacgc agcggaaatc gcagcggtcg gtcaccgaga
      901 cccggaggta ggtgacatgc cggccgaagg gatcgatcat ggtcgcctgg gacatccgtc
      961 atatctggcc gcacattgcg acggggtcaa ggcccggctc agccgccagg agcgggcacg
     1021 gacagcagcc acgacttgaa cgcggcgatg gcggcagcaa gccgatggcc gggatcggcc
     1081 ggctcgatga cgtgataggc atgatcttcc ttgatcacga cctccgagac ctgaacgaga
     1141 cggccgctgt ccaggtcatc ggcaatgaga agcctggggc aaagcgcaat tccctgcccg
     1201 gcgagggccg cggcccgcag gaggttgaag tcctggaaga tgggccccgg cgccggcccg
     1261 gcatgtttca gtccggcgcg gcgcatgtag gtctgccaat catggcgatt ggtgtcgtgc
     1321 agcaagggcc tgccgagcag ggtctgcggc gagtcgagtg gcccttcgcg gtcgagcagc
     1381 gcccggttgc acacggccac cgtcgccccg tccaggaagc gggtggccct gcagccctcc
     1441 cagtcgccgg tcccccacag gatggcgatg tcgatgtcgt cgaagtccag cgcctggtcg
     1501 tggaaggcgt agacgacctt gaccgagatc tcgggccgtt cggagaagaa gcgggtcagc
     1561 cgaggtatga gccagattgt cgcaatggaa ggcaaggcgg caatcgtcaa ggccggcccg
     1621 gcgtcgctct gggcgattcg cttgcaggcc aagccgatga tgtcgaaagc ctcggccagg
     1681 gacttcgcaa gttcctcgcc cttcggcgtc ggcacggtct ggcggccgcg gcgctcaaaa
     1741 aggggcatac cgaaccagtc ttcgagcagc cggatctgat ggctgaccgc cgattgggtg
     1801 acatgcagct cctcggcggc gcgcgagaag ctctgggatc gcgcggcagc ttccaggcat
     1861 ttgagcgcat tgagcggcgg cagcttgggc atcccgatct ccatgagatt tgctcatgga
     1921 ttatgtgagg gatgatcctt tgactcaagt ccgccttttg ccagacatgc aagactgatc
     1981 tgcatctgta tggaggacac cgtgcccgct ctcgaacgcc ccagcctcgt cgactaccgc
     2041 aacaacggaa aacgcccgaa gccgaccttc tcggcagccg aaatgcagcg gcgtcaggac
     2101 aacatccgcg gctggatggc ccggagcaac gtggatgcct gcctcttcac cagctaccac
     2161 aacatctgct acctgtcgga cttcctctac tgctacttcg gccgcagata cggcctggtg
     2221 atcacccaga agggtgcaac gtcggtgagc gcggccatcg acggcggcca gccggcgcgg
     2281 cggaccttcg gcgacaacgt cacctatacg gactggtcga aggaaaacta ctttacggcg
     2341 gtgcaggggc tggtgaaggg agccaggcgg atcggcatcg agttcgatca cgtgaacctg
     2401 gatttccgca agcagcttca ggacgccttc ccaggagtcg acttcgtcga cgtcgcagca
     2461 ccatcgatgc gcatgcggat gatcaagtcg gcggaggaga tcaagcacat caccaagatg
     2521 acccggattg ccgacatcgg tggtgcagcc gtggtcgaag cgaccgcggt gggcgttccc
     2581 gaacatgagg tggcgctgca ttcgacccag gccatggtgc gcgagatcgc acgggtcttt
     2641 ccgaacgccg aactgatgga tacatggacc tggttccagt cgggcgtgaa caccgatggg
     2701 gcgcataatc cggtgaccag ccgcaagatc cgccgcggcg acattctgag cctgaactgc
     2761 ttcccgatgg tggccggcta ctacgtcgcg ctggagcgca cgctctttgc cgaaacggcc
     2821 tcgaaggaac atctccggct ctgggaggtc aactgccacg ttcatgacga aggaaagaag
     2881 ctgctggttc ccggtgcgaa atgctccgac gtggcaaagg cgctgaacga catctatgcc
     2941 agccatgacc tcctcaagta ccgtaccttc ggctatggcc attccttcgg cgtgctctgc
     3001 cactactatg ggcgggaagg cggcctggaa ctgcgcgagg actgcgacac cgtcctggaa
     3061 cccggcatgg tcgtctcgat ggagccgatg atcatgattc ccgaaggcca gaagggcgcc
     3121 ggcggctacc gcgagcatga cattctcgtg atcaccgaga agggcaaccg caacatcacg
     3181 ggctttccct atgggcccga acacctcatc gtccagccgc atcgcgcggc gcggcgggca
     3241 ggaccggtca agtccagggc ttcccgaaag aagtgattgc cgccggaggg cggcattcgc
     3301 gcgcggggct tgaatgcgac gcgggtggct ctatgatccg gcccagcagc cgcgacgccg
     3361 gccactgtga ggtaagaggg gaatggccag ggacatcgcg accgacgaca tgatggtacg
     3421 cttcgtcgac gtgcagaaga gctacgacgg cgaaacgctt gtcgtgaaga acctcaatct
     3481 cgacatcgcc aagggcgagt tcctcaccat gctggggccc tccgggtctg gcaagacgac
     3541 gacgctgatg atgctggccg gtttcgagcc tgccacccat ggcgagatct acgtgaaggg
     3601 ccagccgatc aacaacgtgc cgccgcacaa gcgcggcatc ggcatggtgt tccagaacta
     3661 cgcgcttttc ccccacatga cgatcaacga gaaccttgcc ttcccgctgc aggtgcgcgg
     3721 cgtgggcaag gcggagaccg aggagcgagt cgccaagatc ctcaagatgg tggagttgcc
     3781 gcagatgggc aatcgccgcc cggggcagct ctccggcgga cagcagcagc gtatcgcggt
     3841 ggcccgcgcg ctggtcttcg agcccgatct ggtgctgatg gacgaaccgc tcggcgcgct
     3901 cgacaagcag ctgcgcgagc agatgcagta cgagatcaag cacattcacg agcggctggg
     3961 tgtcaccatc gtctacgtga cgcatgacca gtcggaagcg ctgaccatgt cgaatcgcat
     4021 cgccgtgttc aatgacggcg tcgtgcagca gctttcgact cccgacgagc tttatgagcg
     4081 tccggtcaac tccttcgtcg cgcagttcat cggcgagaac aacaagctga agggaacggt
     4141 cagcgagatt ggcgccgaca aggttgcgac agtgaagctc gagtccggcg acatcgtgaa
     4201 ggccctggct gtcaatgtga agggcaaggg cgaccgcacg ctgctctccg tccgcccgga
     4261 gcggatcgag atcaatccga caaagaagtc ggctgatgtg atcctcgacg gccggattgc
     4321 cgaattgatc tatctcggcg accatatccg cgcccgtctc gaagtggccg gccatgacga
     4381 cttcatcgtc aaggttccca acaagggcgg tgccgcgacg gtggccgagg ggcgcgaggt
     4441 gaaagtcggg tggcgttcag aggattgccg cgctctggac tacatggatt tccgcaactg
     4501 aggagctgaa ggacatggca aacaggcggg caggcgtcat cctcacccgg ctgattgcga
     4561 gcgttctcgg cctggtgctc atcttcgtgc ctccgccgcc cgggcgggcg caggactacg
     4621 aggtcgacct cgcgcttgtc ctcgcgatcg actgctcctt cagtgtcgac agcgaggaat
     4681 accggctgca gatggagggg cttggccgcg ccttcctgcg ggaggaagtc aaggccgcga
     4741 tccgccaggg cgtcaggcag cgcatcgcag tgacggcggt gcaatggtcc gacgagaaca
     4801 atcagagggt catcctcccc tgggtgatca tcgaaggcaa tgccgatgcc gacgaaatcg
     4861 gcatgcggct ggcctcctct cccagggtgc tggcgcaggg cggcacctcg atctccacgg
     4921 cattgctctt ctccgcgaaa ctgttcgaca cggcacccag cgcagagcgg cgggtgatcg
     4981 atgtctcggg cgatggccgc aacaacatcg ggccgcctac cgccgcggcc cgcgaccggg
     5041 tggtcgcgca gggcatcacc atcaacggcc tctccatcct caacgagtgg gacacgctca
     5101 acctctattt cgaatcccat gtcgtgggag ggcctgggca tttcgtgatt ccggccgcag
     5161 actatgagga ttttgccgaa gcgatcttcc gcaagctgct gcgcgagatc accggtcccg
     5221 gcatatcgta aggtgaaaaa ccctcacgct tgaccctgtc agatcagcca catatcggaa
     5281 ggcacgcatc acctttcgaa agccagatca tgcagccatt caccttgagt tcgatccccg
     5341 aaatccactt tggccctggc cggcttgccg atctgggcgg caaggccgtg acgctggccg
     5401 gacagggcgg ccatgtgctg gtcgtggccg atccggcgct cgcagctctt ggcatcacgg
     5461 ccagggcggt cgagtccctg gcgcaggcgg gcttcgccac ctccgtctac gatgccttga
     5521 agggcgagcc gaaacagtcc gacatcgaca gcgccgcgaa catggcacgg gaagcgaaat
     5581 ccagcctgat cgtcggactt ggcggcggct cggccctcga taccgcaaag ctggtggcct
     5641 gctgcgccgt ttccggccag agtgcggcgg cctatgccct ctgcgccacg ccgctgccgg
     5701 cgcaaccgct gccgatcatc gccgttccga ccactgccgg cacgggctcc gaaaccaccc
     5761 gcgtttcggt ctttgccaat gccgagaggc agaaggtctg ggcctggggc gaggaactga
     5821 agccggacct cgcgattctc gatcctgaac tgacgatcgg cgttccgccg catatcaccg
     5881 ccgcaaccgg cctcgacgcg ctggtccatg ccatcgaggc ctgcaccaac aagcaccgga
     5941 ccgatggcaa tgatctctac tgcctgcagg ccatcacgct gatttcggcc aatctcgaac
     6001 gggcgatcgg gaaccccggc gacattgcgg cacgcggcgc gatgctgctc gggtcatgct
     6061 atgctggcat cggcatcgac aattgcggaa cggcgctcgc tcacaacatc tcgcatgcga
     6121 tcgccgatct cgcacccatt ccacatggcc gcgccaccgg gcttgccatg ctcgccacca
     6181 tcgactgggt ggcggaagga catcctgcag ccttcgcgcg cgtggcccgc gccatgtcgt
     6241 cggtcgacgc cgtctcggcc tatgaccgcc tggtgcgcgc caccggcatc aagatatccc
     6301 tcgaagggga cggcttggac ctgacgaacc ctgaacttct ggcgcggcac a
//
LOCUS       DBNGVR010000338         4294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5231626, whole genome shotgun sequence.
ACCESSION   DBNGVR010000338 DBNGVR010000000
VERSION     DBNGVR010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5231626"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1476)
                     /gene="mltG"
                     /locus_tag="AC16A0_07100"
                     /pseudo
     CDS             complement(46..1476)
                     /gene="mltG"
                     /locus_tag="AC16A0_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
     gene            complement(1570..2835)
                     /gene="fabF"
                     /locus_tag="AC16A0_07105"
     CDS             complement(1570..2835)
                     /gene="fabF"
                     /locus_tag="AC16A0_07105"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3105137.1"
                     /translation="MRRVVVTGLGMVSPLATGVEETWSRMIAGHSGAGRITRFDASDL
                     PCQIAFEVKRGDGSNASFNPEQWMDHKEIRRYDDFIIYAMAAAKQAMKDSGIELKTED
                     ERCRAGVLVGSGIGGLSNIADTTMLMETKGPRRISPFFIPGSLINLAGGLISIEVGCK
                     GPNTAVVTACSTGSHAIGDAARIIALDDADVMIAGGAESAICRIGIAGFIACKALATR
                     FNDAPEKGSRPYDADRDGFVMGEGAGVLVLEELEHARARGAKIYAEVVGYGMSGDAYH
                     VTAPAPDGDGAFRCMQAALKRAGLQAHEIDYVNAHGTSTPLGDEIELRAVERLMGNRI
                     EGLTMSSTKSAVGHLLGAAGAVEAIFSILAMRDGICPPTLNLDNPSVETQVDLVPHVA
                     KKKSINTVLSNSFGFGGTNASLVMRRYDA"
     gene            complement(2949..3185)
                     /locus_tag="AC16A0_07110"
     CDS             complement(2949..3185)
                     /locus_tag="AC16A0_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3105138.1"
                     /translation="MSDVAERVKKIVVEHLGVDADKVKPEASFIDDLGADSLDTVELV
                     MAFEEEFGIEIPDDAAETIQTVGDAVKFIEKSSA"
     gene            3381..3950
                     /locus_tag="AC16A0_07115"
     CDS             3381..3950
                     /locus_tag="AC16A0_07115"
                     /inference="COORDINATES: protein motif:HMM:NF027558.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HIR3105139.1"
                     /translation="MKTLFATLATVLVLASPARADGVLDQILTPFDKARLANFDQSSA
                     AALTEARAGGDPADVAVLDKALAGQAMPMGNPLGAWSCRVIKVGGTLPLTVYPRFKCN
                     WGEDGVGWFLEKTSGSQRTQGHFYDGGPTRLTYVGAGHVAGEAPRRYGQDTTENQVAF
                     AERRAPNRIVLMFPEPQYESKLDILVLER"
     gene            complement(4004..>4294)
                     /locus_tag="AC16A0_07120"
     CDS             complement(4004..>4294)
                     /locus_tag="AC16A0_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3105140.1"
                     /translation="KLQKELGSDDFEVVAISVDRKGAEASAAFLKETGADSLRLYVEP
                     TTAILNDIQALGLPATVLIDRQGREIGRLLGPADWASPEAIALVKAALAEPK"
BASE COUNT          708 a         1384 c         1396 g          806 t
ORIGIN      
        1 gtcttagatc acttgcagga tcagcgcaaa acgctgcgct gtcgatcagc ggcgcggatt
       61 cggcaccgga atggcaacca gcttgccatc cactttcacc aggctgcccg gttcgagatc
      121 ggtcacggcc acctgcgccg caggcttctg caccggcttt gcggctgggg cctgcccggc
      181 agccggggtc tcggtttccg cggctggcgg atcaaccgtg acagaggcga gtgccgccgg
      241 cttgggttgc ggaattggaa ccgcggccgg ccccggcact ggcacggttt ccgtgtcggc
      301 tgttgcaccg ggcagcggtt cggcagcagc ttccgcaacg ggcggttcca ccgagatgtc
      361 cggcagggtc tgggccgtgg caggcggttc ggccgcagga gcgccctcgg caggcggatc
      421 gaccgcggcg gtggaaggct ggacggcttc cgcgccggct tcggcggttc ccggcgtttc
      481 cgactccact tcggctgtgg cgctggcctc ttccgcactg accaggcggc gccagttggc
      541 gacgttgcgg ttatgctctt cgagcgtgct ggcgaaggcg tggccacccg agccatcggc
      601 gacgaagaag agatagtccg tgttcggatg attggccaca gcctcgagcg cggcgcggcc
      661 gggattggcg atcggaccgg gcggcaggcc gccgatggtc taggtgttgt agggcgtggg
      721 cgtgcggata tcggtgcgcg tcaaggcccg gtcgagcttt cccttgccgc ccacgattcc
      781 gtagatgatg gtgggatcgg actgaagccg catgcccttc ttcaggcggt tcatgaagac
      841 ggaggcgacc atcggccgct cgtcagccag cgccgtttcc ttctcgacga tggatgccat
      901 cacgaccagt tcctccttgg acttcagtcc gaggtctgga cgccgtgagg cccagatctt
      961 ttcaagcaac tcgtcctgtg cagcctgcat gtcctccagc agtttctggc gggtcatgcc
     1021 acgcttgaac acgtaggtgt ccggcaggat ggcgccttcg gtggggatcg ccgtgatctc
     1081 gccggtcagg atttcgttct ccatcaggcg ggcgacggcc atctcgctcg tccagccctc
     1141 gggaatgctg atcttgtagc cgatggagcg gccggccgcg atcttggcca tgacgtcgcg
     1201 catcgactcg tccttggcga attcgtactc gcccgccttc agcttgccgc gcgcgccggt
     1261 ggcaaaggcc atcgccgaga agacccgcgc atcgccgatg acgcccgcat cctgcaactg
     1321 ggcggcgatc tcgggcgtgc ccagcccacg ctcgatgacc accaccttgt ttcccgcgag
     1381 aggtccggga agcgtgaact gctgataggc atagaaggcg ccgatacccg ccacgacggc
     1441 cgccagcagg ccgagcagga acagcgacct gaacacacgt gagatggccg accgccgttt
     1501 gcgcggaagc ttgggttcgg ggacgcgctg aggctcgctg gaattggtca agcctgtctc
     1561 cgtgtcgggt caggcgtcgt agcgacgcat gaccagtgat gcattggtac caccgaaacc
     1621 aaaagaattc gacagaactg tgttgatgct ctttttcttc gccacgtgcg ggaccaggtc
     1681 aacctgcgtc tcgaccgagg gattgtcgag gttgagggtc ggcgggcaga tgccgtcgcg
     1741 catggcgagg atcgagaaga tcgcctccac cgcaccggcg gcgcccagca ggtggccgac
     1801 cgcggacttg gtcgaggaca tggtcaggcc ctcgatgcgg ttgcccatga ggcgctccac
     1861 ggcgcgcagc tcgatctcgt cgcccagcgg cgtggaggtg ccgtgcgcat tcacatagtc
     1921 gatttcgtga gcctgaaggc cggcgcgctt gagagcggcc tgcatgcagc ggaaggcgcc
     1981 gtcaccgtcg ggcgcgggcg cggtcacatg ataggcgtcg cccgacatgc cgtagccgac
     2041 gacttcggca tagatctttg cgccgcgcgc ccgcgcatgc tccagttcct caagcaccag
     2101 gacgccagcc ccctcgccca tcacgaatcc atcgcgatcg gcatcatagg ggcgcgatcc
     2161 cttttcaggc gcatcgttga acctggtggc cagggccttg caggcaatga agccggcaat
     2221 gccgatccgg cagatcgccg attcggcgcc ccctgcgatc atgacgtcgg catcgtccag
     2281 cgcaatgatg cgggccgcat cgccaatggc atgcgatccc gtcgaacacg ccgtcacgac
     2341 ggcggtattg gggcccttgc agccgacttc gatcgaaatc agcccaccgg ccaggttgat
     2401 cagagacccc ggaatgaaga aggggctgat acggcgggga cccttggtct ccatcagcat
     2461 ggtggtgtcg gcgatgttgc tcaggccgcc gatgcccgag ccgacgagaa cgccggcccg
     2521 gcagcgctca tcctcggtct tcagttcgat gccggaatcc ttcatggcct gcttcgccgc
     2581 ggccatggca tagatgatga agtcgtcgta gcggcggatt tccttgtggt ccatccactg
     2641 ttccgggttg aaggacgcgt tgctgccgtc gccgcgcttc acctcgaagg caatctggca
     2701 tggcaggtcg gaggcatcga agcgggtgat cctgcccgca cccgaatgcc cggcaatcat
     2761 ccggctccag gtttcctcga cgccggtggc caggggggag accattccga gaccggttac
     2821 tacaacgcgg cgcatgcgcg actgtccttt ctatatgaaa acgcggcggc aggttgactt
     2881 gaaccccctg cctccgcgca agacttactc agatccggca ctcctcgtga ggccggggca
     2941 tccgccgatc aggcggagct cttttcgatg aacttcaccg catcgccgac ggtctgaatg
     3001 gtttcagccg catcgtcggg aatctcgatg ccgaattcct cctcgaaagc catgaccagc
     3061 tcgaccgtgt cgaggctgtc cgcgccgaga tcgtcgatga agctcgcttc cggcttgacc
     3121 ttgtcggcgt cgacgccgag atgctcgacg acgatcttct tcacccgctc agccacatca
     3181 ctcatttgtc tatcgtcctt gcttgttttc tgttccgacg gcaggacagc aggaccctgc
     3241 aagtccccct cgcgccgggg gtttccctac cacagattca gattgcggca aaaggggcaa
     3301 tcggcttcgt tgttttcctt ggggaaattg aaaacaggcc cgtcttgcaa cctttcgggc
     3361 accctgccag catatgggcc atgaagaccc tgtttgccac cctcgccacc gtcctggtgc
     3421 ttgcctcccc ggcccgggcc gatggcgtgc tcgaccagat ccttacccct ttcgacaagg
     3481 cccggctcgc caatttcgac caatcttcgg cggctgctct cacggaggcc cgggctggcg
     3541 gcgatccggc cgatgtggcc gttctggaca aggccctggc gggccaagcg atgcccatgg
     3601 ggaacccgct gggcgcgtgg tcctgccgtg tgatcaaggt gggcggcacc ctgccgctga
     3661 ccgtctatcc ccggttcaaa tgcaactggg gcgaagatgg cgtgggctgg ttcctcgaaa
     3721 agaccagcgg gtcgcagcgg acgcaaggtc acttttatga cggcggcccc acccggctga
     3781 cctatgtcgg cgccgggcat gtcgcgggcg aagcgcctcg cagatatgga caggatacga
     3841 cggaaaacca ggttgccttc gccgagcggc gggctccaaa ccgcatcgtc ctgatgtttc
     3901 ccgagccgca atacgagtcg aagctggaca tccttgtcct ggaacgctga aggcccaggc
     3961 cgtcaggctg gccgggggcc acgcgcgagg atgtcagcgc ggatcacttc ggttcagcca
     4021 gcgcggcctt gaccagggcg atggcttcgg gactggccca gtccgccggt cccagcaggc
     4081 gaccgatttc gcggccctga cggtcgatca ggacggtggc cggaaggcca agggcctgga
     4141 tgtcgttgag gatggcggtc gtcggctcga cgtagagccg gagactgtcg gcaccggttt
     4201 ccttcagaaa ggccgccgag gcttccgcgc ccttgcggtc gacactgatc gccaccacct
     4261 cgaagtcgtc cgagcccagt tccttctgga gctt
//
LOCUS       DBNGVR010000339         2608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_321978, whole genome shotgun sequence.
ACCESSION   DBNGVR010000339 DBNGVR010000000
VERSION     DBNGVR010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_321978"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="AC16A0_07125"
     CDS             complement(<1..685)
                     /locus_tag="AC16A0_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR3105141.1"
                     /translation="MSNAPIPVAILTGFLGAGKTTLLNQLLKDPFLANAAVIINEFGD
                     TGIDHLLVERADENVIEMASGCLCCTIRGDLIDTIHDLLARRSRGEIKAFDRIVIETT
                     GLADPAPVLHAVMSEPGLLAACRLEGVITVVDAFNGMATLDAHPEAVKQVAVADRIVL
                     TKVDLLSGRAGEDMLFAIIARLRKLNPAARLLTTHRNEATAERLFTMGLFDPSKKTPD
                     VRSWLAAEAY"
     gene            complement(682..1581)
                     /locus_tag="AC16A0_07130"
     CDS             complement(682..1581)
                     /locus_tag="AC16A0_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3105142.1"
                     /translation="MINRAGLGIACALAAAALYGFVPNMVRAGFENGVPPVESTFFRT
                     ATVGIVFAIIAVLRGEALHLPRPALGSFAVQCVATLVISVGYLASVQFIPVGLAVIIF
                     FSFPVLIMLAAPLIEGHSPGLARIGIAVFAFAGLGVAVGPSFNDLDIRGILLAAAAAV
                     ACVLQFFSGRSISTYMTPTVFGSLVHLTILPAAFLVALYAGDGVIRIFPGGTASGTGL
                     AFMAGVGAIYVVAYMVHMLSLRFAPASTVAPYYNLEPVMTTLVAAVLLGERLNLNQYL
                     GGGMVLTALIASSLIGLRRATRP"
     gene            complement(1588..>2608)
                     /locus_tag="AC16A0_07135"
     CDS             complement(1588..>2608)
                     /locus_tag="AC16A0_07135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3105143.1"
                     /translation="PSKIGMKPGSSISTNLALQSLLIYSANDMAYVLAEGAAGSVDVF
                     VREMNATAQKLGMSGTRFANPNGLFDPRQITTARDIAILATALLKTYPERAHYFSQDA
                     VAIGKRRLPNRNSLLRQMKAADGMKTGFVCNSGFNLVATATIDGRRLGAVIFGASSGK
                     HRADLAEMLLVDAFGRSDPPQRQPLSGIANMALGGIVPADLTTTICRQKAPVQLVSSK
                     ELQGWGISFGTYDTAVKADMALRGRLLSASAAGLSGPAGVIRMPGKAGFAAVVWKLAE
                     PQSLTACASYRQEQSPCDVLTPETFAQIAALTPEPPPKPKAQSVQGSDSGKTKKKKKN
                     TKKKP"
BASE COUNT          474 a          816 c          885 g          433 t
ORIGIN      
        1 cataggcttc ggccgccagc cagctgcgca cgtccggcgt cttcttcgat ggatcgaaga
       61 gacccatggt gaagagccgt tcggcggtgg cctcgttgcg gtgggtggtc agcagccgtg
      121 cggccggatt cagtttccgc aaccgcgcga tgatggcgaa gagcatgtcc tcgcccgcgc
      181 ggcccgaaag cagatcgacc ttggtcagca cgatacggtc ggcgaccgcc acctgcttca
      241 ccgcttccgg atgggcgtcc agcgtcgcca tgccgttgaa ggcatccacc accgtgatca
      301 cgccctcgag gcggcaggcg gccagcaggc ccggctcgct catcaccgca tgcaggacag
      361 gcgccggatc ggcgagtccc gtcgtctcga tgacgatgcg gtcgaacgcc ttgatctcgc
      421 cgcggctgcg ccgcgccagg agatcgtgga tcgtgtcgat cagatcgccg cggatggtgc
      481 agcacaggca gcccgaggcc atttcgatga cattctcgtc ggcacgttcg accagcagat
      541 gatcgatgcc ggtatcgccg aactcgttga tgatgacggc tgcattggca agaaagggat
      601 ccttgagcag ctggttcaac agcgtcgtct ttccggcgcc gaggaagccg gtcaggatgg
      661 cgacggggat gggtgcgtta ctcatggtcg tgtggccctg cggagtccga tcaggctgga
      721 ggcgatcagg gcggtcagca ccatgccgcc gcccagatac tggttcaggt tgagccgctc
      781 cccgagcagc acggcagcaa ccagcgtggt catcaccggc tcgaggttgt agtaaggcgc
      841 caccgtcgag gccggcgcga aacgcagcga cagcatgtga accatatagg ccacaacata
      901 gatggcgcca acgcccgcca tgaaggcgag tcccgtcccc gatgcggtgc caccggggaa
      961 aatccggatg acaccgtcgc cggcgtaaag cgcaaccaga aaggccgccg gcaggatggt
     1021 gaggtgcacc aggctgccga agaccgtcgg cgtcatgtag gtggagatgg agcggccgga
     1081 aaagaactgc aacacgcagg ctacagcggc ggcggccgcc agcaggatcc cacggatgtc
     1141 caggtcattg aagctcggtc ccacggcgac gccaagcccg gcgaaggcga agacggcgat
     1201 gccgatgcgc gccagccccg gcgaatgacc ctcgatcagg ggggccgcaa gcatgatgag
     1261 caccgggaac gagaagaaga tgatgaccgc gaggcctacc ggaatgaact gcacggaggc
     1321 gaggtagcca accgagatga ccagggttgc cacgcactgc accgcgaagc ttcccagcgc
     1381 cggacggggc aggtgcaggg cttcgccgcg cagcaccgca atgatggcga agacgatgcc
     1441 aacggtggcg gtgcggaaga acgtgctctc caccggcggc acgccattct cgaatccggc
     1501 ccgcaccatg ttcggcacga agccgtaaag tgcggctgcc gcgagcgcgc aggcgatgcc
     1561 aagtcccgcc ctgttgatca tgatgcctca gggcttcttc ttggtgttct tcttcttttt
     1621 cttcgtcttg ccggaatccg aaccctgcac gctctgcgcc ttgggtttcg gcggcggctc
     1681 gggcgtcagg gctgcgatct gcgcgaaggt ctcgggcgtg agcacgtcgc agggcgattg
     1741 ttcctggcga tagctggcgc aggctgtgag gctctggggc tcggcgagtt tccagaccac
     1801 ggcggcaaac ccggccttgc cgggcatgcg gatgaccccg gccggtcctg acagtccggc
     1861 agccgaagcg ctgagcaggc ggccgcgcaa ggccatgtcg gccttcacgg ccgtgtcata
     1921 ggtaccgaag gaaatgcccc agccctgcag ctccttcgag ctgaccagct gcactggcgc
     1981 cttctggcgg cagatcgtgg tggtcaggtc ggccggcacg atgccgccca acgccatgtt
     2041 ggcgatgccc gacagcggct ggcgttgcgg cgggtcgctg cggccgaagg catcgaccag
     2101 cagcatctcg gcaagatcgg cacgatgctt tccgctgctc gccccgaaga tcaccgcgcc
     2161 gagcctgcgg ccatcgatgg tggccgtggc caccaggttg aatccggaat tgcagacgaa
     2221 gccggtcttc atgccgtcgg ccgccttcat ctggcgcagc agcgaattgc ggttgggcag
     2281 tcggcgcttg ccgatggcga cggcatcctg cgaaaagtaa tgtgcccgtt cgggataggt
     2341 cttgaggagc gcggtggcca ggatcgcgat gtcgcgcgcg gtggtgatct ggcgcggatc
     2401 gaaaagcccg ttgggattgg cgaaacgcgt gcccgacatt cccagttttt gcgccgtggc
     2461 gttcatctcg cgcacgaaga catcgaccga ccctgcggcg ccctccgcca gcacataggc
     2521 catgtcgttg gccgaataga tcagcagcga ctgcaatgcc agattggtgc tgatgctgct
     2581 gcccggcttc atgccgatct tgctgggt
//
LOCUS       DBNGVR010000340         4158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5231680, whole genome shotgun sequence.
ACCESSION   DBNGVR010000340 DBNGVR010000000
VERSION     DBNGVR010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5231680"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="AC16A0_07140"
     CDS             <1..494
                     /locus_tag="AC16A0_07140"
                     /inference="COORDINATES: protein motif:HMM:NF028177.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HIR3105144.1"
                     /translation="LPAALAVLRSSEPQLAEGIATVAPLLGWTTYDVYPHELIGTDFA
                     DGHAFGSVIGEGAPLPAADYDFGLFLIAPHVLYRDHAHKAPELYAPLTGPHGWRFGPG
                     LPLEIMPAHQPVWNEPYRPHLTKIGPTPFLCLFCWTRDVDEPAVVLPADDWTALEALR
                     LGS"
     gene            496..2130
                     /locus_tag="AC16A0_07145"
     CDS             496..2130
                     /locus_tag="AC16A0_07145"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3105145.1"
                     /translation="MSADTILINAHVRTMDAARPRAEALAIRDGRILALGSTAEMRGL
                     ATQRTRVIDAGGRLVLPGFQDAHVHLLLGGADLVTSAQLYLVTTVGELQSVLRAHAES
                     HRSLTVVQGAGWQPGLFGEHNLTRHVIDAVVPDRPCLIIDSSGHNACLNSVAMAMIGL
                     VKGTPDPVNGHFVIDGGGEPTGMLHEDAIFWAIDRLPALSDADDDAGLRAGQAHANAH
                     GITGIIDPRVTEREARIYGSGAARGDLTLRVSGAALVKESDTPVTAVERLTALRRQHP
                     GPDFWVQSAKFFLDGVFENRTAALLEPYADAPGGNCDVMFQPAQVNELFAALDAARFQ
                     IHVHVIGDRATRVALDGLEAALAANGRWPSLHQLAHLQLVHPDDFARIGALGVMANIQ
                     TLWARLDPMIPDIALDMIGSARRPQVYAFRRMLDAGAPYCLSSDWSVSTLDPFAIMET
                     AVTRQARLADGARESFLPGEALTIEEAVLGYTAHAAAACWRGDSTGRLLPGFSADIIV
                     TDRDLFAIPPHEIGDTRVLLTLFKGREVHRHEDFAG"
     gene            2333..3028
                     /locus_tag="AC16A0_07150"
     CDS             2333..3028
                     /locus_tag="AC16A0_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3105146.1"
                     /translation="MSRLTVESLRTITVFKPLSDLAFADLSQRCVMRHLDPGELVIGH
                     TDQTLDVYFLLGGLARVSIYSADGQRVGFRDLTAGAIFGELSAIDSKPRSASVECVDP
                     CSVVTMRREHFLDALGKYPAFSLAVACHLTAQIRSLTTRVFEYSTLAVRNRVRLELLR
                     LLDAASDNGSAGLLTQVPTHAEIASRISTHREAVSREVAWLEHNKYILKEGRNLRIVD
                     PAALRALVDEDWS"
     gene            complement(3025..3738)
                     /locus_tag="AC16A0_07155"
     CDS             complement(3025..3738)
                     /locus_tag="AC16A0_07155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3105147.1"
                     /translation="MSLWPVGCFHERCVLVTGGTSGIGLAIAEGFAAEGARVIAAGLE
                     KIARPGVTGVLLDVRDRTAVDRLIGGLDRLDVVVNCAGLIRRPEEHDPDVFDAVVDVN
                     LSGTMRVCAAARPKLATAQGSIVNIASMLSFFGSGPAPAYAASKGGVAQLTKSLAIAY
                     AAEGIRVNAVAPGWIETPLTAALRADPEKHAAIQSRTALKRWGKPEDVTGGVLFLASP
                     LARYITGTILPIDGGYLAV"
     gene            complement(3752..>4158)
                     /locus_tag="AC16A0_07160"
     CDS             complement(3752..>4158)
                     /locus_tag="AC16A0_07160"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HIR3105148.1"
                     /translation="QAENVWFRPLCLNRSQGYWMNLLRVRKAGVLSRHRHPNAVHGLV
                     LKGRWHYLEHDWVATEGSYVFEPPGETHTLVVPDDVEEMITFFQVNGIMYYVDPWGKP
                     LGYEDVFTKIDMCRSHFDKVGLGADFVDQFIR"
BASE COUNT          692 a         1399 c         1300 g          767 t
ORIGIN      
        1 atctgccggc agcgcttgcc gtcctgcgca gcagcgagcc gcaattggca gaaggcatcg
       61 cgacggttgc gccgcttctc ggctggacga cctatgacgt ctacccccat gaattgatcg
      121 gcacggactt cgccgatggc cacgccttcg gctcggtgat cggcgagggc gcgccgctgc
      181 ccgccgctga ctatgatttc ggcctcttcc tcatcgcgcc ccatgtgctt taccgcgatc
      241 atgcccacaa ggcgccggag ctctatgcgc ctctcaccgg accgcatggc tggcgtttcg
      301 ggccgggatt gccgctcgag atcatgccgg cgcaccagcc ggtgtggaat gagccctacc
      361 ggccgcatct caccaagatc ggtcccacgc cctttctctg cctcttctgc tggacccgcg
      421 acgtagacga gccggcggtg gtcctgccgg ccgatgactg gaccgcgctt gaagcgctgc
      481 gcctcgggag ctgacatgag cgccgacaca attctgatca acgcccatgt ccgcaccatg
      541 gatgcggcgc gcccacgggc cgaggcactg gcgatccgcg atggccgaat cctcgcgttg
      601 ggcagcacgg ctgagatgcg cgggcttgcc acgcagcgca ccagggtgat cgatgccggc
      661 gggcgactgg tcctgccggg ctttcaggac gcacatgtcc atctcctgct cggcggtgcc
      721 gatctcgtga cctcggcgca gctctacctc gtcaccaccg tcggcgaact tcagtcggtc
      781 ctgcgggccc atgccgaaag ccaccggagc ctgacagtcg ttcagggggc cggctggcag
      841 cccgggttgt tcggcgagca caatctcacc cgtcatgtca tcgatgccgt cgtgccggat
      901 cgcccgtgcc tgatcattga ctcgagcggt cacaatgcct gcctcaactc ggttgccatg
      961 gccatgatcg gactggtcaa gggaacacct gacccggtca atggtcactt cgtcatcgac
     1021 ggcggcggcg aacccaccgg catgctgcat gaggacgcca tcttctgggc gattgaccgc
     1081 ctgccggcac tttcggatgc cgacgacgac gccggactcc gtgcggggca ggctcatgcc
     1141 aatgcccacg gaatcaccgg catcatcgat ccgcgcgtca ccgaacgaga ggcccgcatc
     1201 tatggttcgg gtgctgcgcg tggcgacctc acgctgcggg tttccggcgc ggccctggtg
     1261 aaggagagcg acacgccggt cacggctgtc gagcggctca cggccttgcg caggcaacat
     1321 ccgggtcccg acttctgggt gcaatcagcc aagttctttc tcgacggcgt cttcgagaac
     1381 cgtaccgcgg ccctgcttga accttatgcc gatgcgccgg gcggcaattg cgatgtcatg
     1441 ttccagccgg cacaggtgaa tgagctgttt gcggccctcg atgcggcgcg cttccagatc
     1501 catgtccatg tcatcggcga tcgcgccacc agggtcgcac tcgatggatt ggaagccgca
     1561 ctcgccgcca atggccgttg gccgtcgctg catcagctgg cgcatctgca gcttgtccat
     1621 ccggatgact tcgcccgcat cggtgccctt ggcgtcatgg cgaacatcca gactctctgg
     1681 gcgaggctcg acccgatgat ccccgatatt gcgctggaca tgatcggtag cgcacgccgg
     1741 cctcaggtct atgctttccg ccgcatgctc gatgccggcg cgccctactg cctgtcatcc
     1801 gactggtcgg tctccacact tgatcccttc gccatcatgg agacggccgt cacccgccag
     1861 gccaggctag cggatggcgc gcgcgaatcc ttcctgccgg gcgaggccct gacgatcgaa
     1921 gaggccgtgc ttggctatac ggctcatgcc gccgcagcct gctggcgcgg cgacagcacc
     1981 ggacgactgc tgcccggatt ttcggcagac atcatcgtga ccgaccgcga cctcttcgcc
     2041 attccgccgc atgaaatcgg cgacacgcgc gtgctgctga cgctgttcaa ggggcgcgag
     2101 gttcaccgtc acgaggattt cgcgggctga cgggttgttt ccgtcacggc atcgctgcca
     2161 ttttgataca cgtaaagttt tagcgaaacg gcacggctgc ttccgcgtgt tgccgtgatc
     2221 gcggttgcaa aacatgaccc gatgcatcta ctatgcagcg gctctcaagt catccgtata
     2281 cagagcgaat ggcttgggcg cgggtagaga cgcggttggg gctgacagcg gcatgagcag
     2341 gttaacggtt gagtcgcttc ggaccatcac ggtcttcaag ccgctgtccg atctggcctt
     2401 tgccgacctg agccagcgct gtgtcatgcg ccatctcgat ccgggcgaac tggtcatcgg
     2461 ccataccgat cagacgcttg acgtctactt tctgctgggc ggcctggcgc gcgtcagcat
     2521 ctactcggcc gatggccagc gtgtcggctt tcgcgatctc accgccgggg ccatcttcgg
     2581 cgagttatcg gcgattgaca gcaagccgcg ctcggccagt gttgaatgcg tcgatccctg
     2641 cagcgtggtg accatgcggc gcgaacactt cctcgacgcg ctgggcaagt acccggcatt
     2701 ttcccttgcg gtcgcctgtc acctgacggc gcagatccgc tcgctcacca cgcgtgtctt
     2761 cgaatacagc acgctggcgg tccgcaaccg cgtgcgcctc gagctgttgc gcttgctcga
     2821 tgcagcgtcc gacaacggct cggccggact cctgacacag gtgccaaccc atgccgagat
     2881 cgcgagccgc atcagcaccc accgcgaggc cgtctcgcgc gaggtcgcct ggctggagca
     2941 caacaagtac atcctgaaag agggccggaa cctgcgcatc gtcgatcccg cagcgctgcg
     3001 tgccctggtc gacgaggact ggagctagac ggccagatag ccaccgtcga tgggcaggat
     3061 ggtgccggta atgtaacggg cgagcggcga tgcgaggaag agcacgccgc ccgtcacgtc
     3121 ttcgggcttt ccccagcgtt tcagcgctgt ccgcgactgg atcgccgcat gcttttcggg
     3181 atcggcccgc agtgcggcgg ttagcggtgt ctcgatccag ccgggagcca cggcattcac
     3241 ccttatcccc tcggcggcat aggcgatggc gagcgacttg gtaagctggg ccaccccgcc
     3301 cttcgaagcc gcataggccg gcgccgggcc gctgccgaag aagctgagca tggaggcgat
     3361 gttgacgatc gacccctgtg ccgtcgcaag cttcgggcgt gcggccgcgc agacccgcat
     3421 ggtgcccgag agattgacgt cgaccacggc gtcgaagaca tcgggatcgt gctcctccgg
     3481 gcgtcggatc agtcctgcgc agttcaccac cacgtcgaga cggtccagtc ccccgatgag
     3541 gcgatcaacg gccgtcctgt cgcggacgtc gagcaggacg cccgtcacgc cgggccgcgc
     3601 gatcttttca agtcccgccg cgatgacccg cgcgccctct gccgcgaagc cctcggcgat
     3661 ggccagcccg atgccggatg tgccaccggt caccagcacg cagcgctcat ggaagcagcc
     3721 gacaggccac aggctcatgc gatggtctcc gtcaacggat gaattggtcg acgaaatccg
     3781 caccgagccc gaccttgtcg aaatgagagc ggcacatgtc gatcttggtg aagacatcct
     3841 cgtagcccag aggttttccc caaggatcga cgtagtacat gatcccgttg acctgaaaga
     3901 aggttatcat ctcctccacg tcgtccggca ccaccagcgt gtgggtttcg cccggcggct
     3961 cgaagacata gctgccttcg gtcgccaccc agtcgtgttc gagatagtgc cagcggccct
     4021 tcagcacgag gccgtgcacc gcattgggat ggcggtggcg cgacaggacg cccgccttgc
     4081 gcacgcgcag aaggttcatc cagtagccct gggaccggtt gaggcagagc ggccggaacc
     4141 agacattctc cgcctgcg
//
LOCUS       DBNGVR010000341         3281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1894603, whole genome shotgun sequence.
ACCESSION   DBNGVR010000341 DBNGVR010000000
VERSION     DBNGVR010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1894603"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16A0_07165"
     CDS             <1..461
                     /locus_tag="AC16A0_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3105149.1"
                     /translation="PFRRAIEEGVLDPRRTIQIGLRGSMYDLDDFDYGAKMGVRLVRI
                     EEAMEKGPKAIMADARRIVGDGPVYVSFDIDMLDPVYAPGTGTPEIGGFTTFQAQQML
                     RELRGLNIVGADVVEVSPPFDPSGLTAHAGVTMMFEILCSMAEDVAKRRR"
     gene            complement(474..1082)
                     /locus_tag="AC16A0_07170"
     CDS             complement(474..1082)
                     /locus_tag="AC16A0_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HIR3105150.1"
                     /translation="MRRDEPYSEIVPARAHWSRFVGKGETLRIVDTHGQQAVDFLCYN
                     AADPEERYHHPNTIKKAGRILLTKGDVLYSDRARPLFTITADSFGGHDTIGGCCSQPS
                     NLMLYGVSNTGCRENFLAALKPYGLGWRDIVPNVNWFMTVPVGAAGAAAIAKGASKPG
                     DHVDLRAEMDVLAVISNCPQVLNPCNNYNPTPIEVIVLPAAK"
     gene            complement(1082..1888)
                     /locus_tag="AC16A0_07175"
     CDS             complement(1082..1888)
                     /locus_tag="AC16A0_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR3105151.1"
                     /translation="MNQALREVKPEQDMILIEHLPFQTDSGIASRARIGLIVLATDFT
                     IEHEWRKIITPLAGVALYQSRILNDAQITPETLRAMEPRIASATDVILPGGELDVVAY
                     GCTSASMAIGEEKVFQRIREVRPGAKGTTPITAAFAAFRAFGAKRIGVLTPYRADVNR
                     IVASYIQARGFEVPVFGSFNEQDDSIVARITPESIRSGVRRLLDTAKVDCVFVSCTSV
                     RLAEAARDIEAETGVPITSSNHAMAWHALRLAGVSDALPQWGRLFTRPLD"
     gene            1957..>3281
                     /gene="lnt"
                     /locus_tag="AC16A0_07180"
     CDS             1957..>3281
                     /gene="lnt"
                     /locus_tag="AC16A0_07180"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3105152.1"
                     /translation="MIRLRQACRKLRGWRRGGVTVLAGALAALALPPLGAWPLLFAAV
                     PLFLAVLEGATTRRSALLLGWLFGLGYFVVAFHWIGFAFLVDADTYLWMMPFMVGGLA
                     GGMAIYWGLAALAVRLARLHGLSLVIGFAAAIAVAEWLRGHLFTGFPWAAPGLAAEAM
                     GPVAQVASVTGMTGLTLLILLLAGLPLVLLSPSLRRERWLALALLALLPAGWGFGAWQ
                     LALPKTPDTELSLRIVQPNVPQSDKWRSENARMIFDRLQELSLAPTADRPAGLAGIDY
                     LIWPESAVPFLIDESPVARDEIARMLNGKGVLLTGSIRREAQSGDTPARVFNSIIAFD
                     GRGDVVARYDKWRLVPGGEFLPFEWLLEPLGFRKVVTVPGSFAAGPGPQTLQLPGGLA
                     AAMLVCYEVIFPHDLIQPGARPAVIVNVTNDGWFGQSTGPHQHLAQARMR"
BASE COUNT          484 a         1066 c         1132 g          599 t
ORIGIN      
        1 cgcccttccg ccgcgccatc gaggaaggcg tgctggatcc caggcgcacc atccagatcg
       61 gcctgcgcgg ctccatgtac gatctcgacg acttcgacta tggtgcgaag atgggcgtgc
      121 gcctcgtgcg catcgaagag gcgatggaaa agggccccaa ggccatcatg gccgatgcgc
      181 gccgtatcgt gggcgacggc cccgtctatg tgagcttcga catcgacatg ctcgatcccg
      241 tctatgcgcc gggcaccggc acgcccgaga tcggcggctt caccaccttc caggcccagc
      301 agatgctgcg cgagctgcgc gggctgaaca tcgtcggcgc cgacgtggtg gaagtctcgc
      361 cacccttcga cccgtccggc ctcacggccc atgcgggcgt caccatgatg ttcgagatcc
      421 tctgctccat ggccgaggat gtggcgaagc ggaggcggta atctgctgga tcgtcacttc
      481 gcggcgggca ggacgatcac ttcgatgggg gtcggattgt agttgttgca ggggttcagc
      541 acctgcgggc agttcgagat caccgccagc acatccattt cggcgcgcag atcgacatgg
      601 tcgccgggct tcgacgcgcc cttcgcgatg gcggccgcgc cggccgcgcc caccggcacg
      661 gtcatgaacc agttcacgtt gggcacgatg tcgcgccagc cgaggccata gggcttcagc
      721 gcggcgagga agttctcgcg gcagcccgtg ttcgacacgc catagagcat gaggttcgat
      781 ggctgcgagc agcagccgcc gatggtgtcg tgtccgccga agctatccgc cgtgatggtg
      841 aagagcggcc gggcgcgatc cgaatagagc acatcgccct tggtcaggag aatgcggccg
      901 gccttcttga tcgtgttggg atggtgatag cgctcctccg gatcggcggc gttgtagcag
      961 aggaaatcca ccgcctgctg gccatgcgtg tcgacgatgc gcagcgtctc gcccttgccg
     1021 acgaagcggc tccagtgcgc acgcgcaggg acgatctcgc tgtaaggctc gtcgcggcgc
     1081 atcagtccag tggccgggtg aagaggcggc cccattgcgg cagggcatcg ctgacgccgg
     1141 ccagccgcag cgcgtgccag gccatggcat ggttggacga ggtgatcgga acgccggtct
     1201 cggcctcgat gtcgcgcgcc gcttccgcca gcctgaccga ggtgcatgag acgaagacgc
     1261 agtcgacctt tgccgtatcc agcaggcggc gcacgccgct gcggatcgac tccggcgtga
     1321 tgcgcgccac gatgctgtcg tcctgctcgt tgaaggagcc gaagaccgga acctcgaagc
     1381 ctcgcgcctg gatgtagctg gcgacgatgc ggttcacgtc ggcgcggtag ggcgtcagca
     1441 cgccgatgcg cttggcgccg aaggcgcgga aggccgcgaa ggctgcggtg atcggcgtgg
     1501 tccctttcgc gccgggacga acctcccgga tccgctggaa gactttctcc tcgccgatcg
     1561 ccatcgaggc ggaggtgcag ccataggcaa ccacatcgag ttcgccgccg gggagaatga
     1621 cgtcggtcgc ggacgcgatg cgtggctcca tggcgcgcag cgtctcgggc gtgatctggg
     1681 catcgttgag gatgcggctc tggtagagcg ccacgccggc gagcggcgtg atgatcttgc
     1741 gccactcgtg ctcgatggtg aagtcggtcg ccagcacgat cagtccgatg cgggcgcggc
     1801 tggcgatgcc gctgtcggtc tggaagggca ggtgctcgat caggatcatg tcctgttccg
     1861 gcttcacctc gcgcaaggcc tggttcatgc ggcaactcct cggctgtcgc gcgatttgta
     1921 ccagagatcg gcaggcacac ctatagtgcg ggaaccatga ttcggctgag acaggcatgc
     1981 cgtaaactca gaggctggcg gcgcggcggc gtcaccgtcc tggctggtgc gctggccgcg
     2041 ctggccctgc cgccgcttgg cgcatggccg ctgctcttcg ccgccgtgcc cctgttcctt
     2101 gccgtgctgg aaggcgccac cacgcgccgg tcggcgctgc tcctcggctg gctcttcggc
     2161 cttggctatt tcgtggtggc ctttcactgg atcggctttg ccttcctggt cgatgccgac
     2221 acctatctgt ggatgatgcc cttcatggtc ggcggacttg ccggcggcat ggccatctac
     2281 tgggggctcg cggcgcttgc cgtgcgactg gcccgcctgc atggtttgag cctggtcatc
     2341 ggcttcgcag ccgccatcgc cgtggcggaa tggctgcgcg gccatctgtt caccggcttt
     2401 ccctgggcgg cgccgggcct cgcggccgag gcgatgggac cagtggccca ggtggcctcg
     2461 gtcaccggga tgacgggcct cacgttgctg atcctgctgc tggccggatt gcccctggtg
     2521 ctgctgtcgc ccagcctccg ccgtgagcgg tggctggcgc tcgccctgct tgcgctgctt
     2581 ccagcggggt ggggatttgg cgcatggcag ctcgcactgc cgaaaacgcc cgacaccgag
     2641 ctgagcctgc gtatcgtgca gccgaatgtt ccgcaaagcg acaagtggcg cagcgagaat
     2701 gcgcggatga tcttcgacag gctgcaagag ctcagcctcg cacccaccgc ggaccggccg
     2761 gcggggcttg ccggcatcga ctacctcatc tggccggaat cggcggtgcc cttcctgatc
     2821 gacgagagcc cggtcgcgcg cgacgagatc gcccgcatgc tgaatggcaa gggcgtgctg
     2881 cttaccggat cgattcgccg cgaggctcaa tctggagata cgcctgcgcg cgtcttcaac
     2941 agcatcatcg ccttcgatgg caggggcgat gtggtcgcgc gctatgacaa gtggcggctc
     3001 gtgccgggcg gcgaattcct ccccttcgaa tggctgctcg agccgctcgg cttccgcaag
     3061 gtggtgaccg ttccgggcag tttcgcggcc ggccccgggc cccagacgct gcaattgccc
     3121 ggcggcctcg ctgccgcgat gctcgtttgt tacgaagtga tttttccgca tgacctgatt
     3181 cagcccggcg cccggcctgc cgtgatcgtc aacgtgacca acgatggctg gttcggccag
     3241 tcgacgggcc cgcaccagca tctggcgcag gcccggatgc g
//
LOCUS       DBNGVR010000342         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3270213, whole genome shotgun sequence.
ACCESSION   DBNGVR010000342 DBNGVR010000000
VERSION     DBNGVR010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3270213"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="modA"
                     /locus_tag="AC16A0_07185"
     CDS             <1..417
                     /gene="modA"
                     /locus_tag="AC16A0_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3105153.1"
                     /translation="KDFPLTTLLGDGKLAMGDVKAVPAGKYGKAALVSLGVWSSVEGK
                     VAQAENVRAALKLVATGEAPLGIVYQTDARAEPKVKVVGTFPSDSHPPIIYPVAVTAE
                     SKNPEATALVTFLQSAKAQDIFKAQGFTILVPPATN"
     gene            421..1119
                     /gene="modB"
                     /locus_tag="AC16A0_07190"
     CDS             421..1119
                     /gene="modB"
                     /locus_tag="AC16A0_07190"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR3105154.1"
                     /translation="MTWLELSPDEWSAVRLSLKVATVAMLASLPFGIAVALLLARRDF
                     WGKSLVNGLVHLPLVLPPVVTGYLLLLTFGKRGPVGSFLNDAFGIVFSFRWTGAALAC
                     AVMGFPLMVRAIRLSIEAVDRRLEAAASTLGANALWVFLTVTLPLTLPGIVAGMILCF
                     AKAMGEFGATITFVSNIPGETQTLPSAIYTFTQVPGGDAGALRLTAISVAISLAALMA
                     SEVLARRVGRRIAA"
     gene            1116..2207
                     /gene="modC"
                     /locus_tag="AC16A0_07195"
     CDS             1116..2207
                     /gene="modC"
                     /locus_tag="AC16A0_07195"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HIR3105155.1"
                     /translation="MSVAVAVRHRLGDFALDSAFESQGRLTAIFGASGSGKTTLINVI
                     AGLIKPDWGRIVIDGAVLTDTEEHLAVPVHRRRIGYVFQDSRLFPHLTVEQNLHYGRW
                     FAPRGDRYAQTAQVLALLGIGHLLTRKPSQLSGGERQRVAIGRALLASPRLLLMDEPL
                     ASLDQPRKEEILPYIERLRDDLRLPILYVSHSLAEVKRLATDIVVMAEGRVAASGPAD
                     RIAQRLDLVPPEERDEGGVILDMTVTGYDQAYDMTSLRSAAGEAHVQGKMGKPGAVLR
                     VQIRARDVMVATRKPTKISALNIFRGRIAALDAAGPSAVNVTIDCAESPIIARITRQS
                     RDALQLNPGLEVYALVKAISVSPPAAAPC"
     gene            complement(2204..2548)
                     /locus_tag="AC16A0_07200"
     CDS             complement(2204..2548)
                     /locus_tag="AC16A0_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3105156.1"
                     /translation="MAQLSIRIDFGPGLRIGPGKIQLLEEIAALGSIAAGGRALRMSY
                     RRAWELIEDLNKTFGKPVVESKSGGKKGGGATLTPLGLALISRYRAMERAAASATEAH
                     LEALKREIARAS"
BASE COUNT          453 a          907 c          812 g          492 t
ORIGIN      
        1 aaggactttc ccctgaccac cctgctcggc gacggcaagc tcgccatggg cgatgtcaag
       61 gccgtgccgg caggcaaata cggcaaggcg gctctggtgt cgctgggcgt ctggtcctcc
      121 gtcgagggca aggtcgccca agcggagaac gtccgcgccg cgctgaagct ggttgccacc
      181 ggcgaggccc cgcttggcat cgtctaccag acggatgcga gggccgaacc caaggtgaag
      241 gtcgtcggaa ccttcccatc tgacagccac ccgccgatca tttatccggt cgccgtcacc
      301 gctgaatcga aaaacccgga agccaccgcg ctggtgacat tcctgcaatc cgccaaggcg
      361 caggacatct tcaaggccca gggcttcacg atcctggtgc cccctgctac caactgagcc
      421 atgacatggc ttgaactcag cccggatgaa tggagtgccg tccggctgag cctcaaggtg
      481 gccaccgtcg cgatgctggc cagcctgccc ttcggcatcg ccgtagcgct gctcctggcg
      541 cgccgcgact tctggggcaa gtcgctcgtc aacgggctgg tgcatctgcc gctggtgctg
      601 ccgccggtcg tgaccggcta tctgctgctc ctgaccttcg gcaagcgtgg accggtcggc
      661 tccttcctca acgacgcctt cggcatcgtc ttctccttcc gctggacggg agctgcactc
      721 gcctgcgcgg tcatgggctt tccgctgatg gtgcgcgcca tacggctctc catcgaggcg
      781 gtcgaccgca ggctggaagc cgccgcatcg acgctgggcg ccaatgcgct gtgggtcttt
      841 ctgaccgtga ccttgccgct caccctgccc ggcatcgtgg ccggcatgat cctgtgcttc
      901 gccaaggcca tgggcgagtt cggcgcgacc atcaccttcg tatccaacat tccaggcgag
      961 acccagacac tgccctcggc catctatacc ttcacgcaag ttcccggcgg cgacgccgga
     1021 gccttgcgtt tgacggccat ttccgtggcg atctccctgg ccgccttgat ggcatccgag
     1081 gtgcttgcac ggcgtgtcgg ccggcgcatc gccgcatgag cgtcgcagtc gcagtccgcc
     1141 accgactcgg tgatttcgct ctcgattccg cgttcgagag ccagggccgg ctcacagcca
     1201 tcttcggcgc ctccggctcg ggcaagacca cgctcatcaa tgtcatcgcc gggctgatca
     1261 agccggattg gggaaggatc gtcatcgacg gcgccgttct gacggacacg gaagaacatc
     1321 tcgccgtacc cgtgcatagg cgccgcatcg gctatgtctt ccaggactcg cgcctctttc
     1381 cgcacctcac tgtcgagcag aatctccact atggccgctg gtttgcgccg cgcggcgacc
     1441 gctatgcaca aacggcgcag gttctggcct tgctgggcat tggtcatctg ctgacgcgca
     1501 agccgagcca gctgtccggc ggcgagcgcc agcgcgtcgc gattggccgg gccttgctcg
     1561 cttcgccgcg cctcctgctc atggacgagc cgctcgcgtc gctggaccag ccgcgcaagg
     1621 aagagatcct cccatacatc gaacgcctgc gcgatgacct gcgccttccc attctctatg
     1681 tcagccattc gctcgcagag gtgaagcgcc tcgccaccga catcgtggtg atggccgaag
     1741 gccgtgtcgc agcttccggg cctgcggatc ggattgcaca gcgcctcgat ctcgtgccgc
     1801 ccgaagagcg cgacgaaggc ggcgtcattc tcgacatgac ggtgaccggt tacgatcagg
     1861 cctatgacat gaccagcctg cgttcggcgg cgggcgaggc gcatgtgcag ggcaagatgg
     1921 ggaaaccggg cgcagtgctc cgcgtgcaga tccgcgcccg cgacgtgatg gtggccaccc
     1981 ggaaacccac gaagatcagt gccctgaaca tcttccgcgg acggatcgcg gcgctcgatg
     2041 cggcgggacc ttctgccgtg aacgtgacca ttgattgcgc cgaatcgccc atcatcgcgc
     2101 gcatcactcg ccagtcgcgc gatgcgctgc agctcaatcc cggcctggag gtctatgcgc
     2161 tggtgaaggc catatcggtc agtccacccg ctgccgcacc ctgctagctg gcgcgggcga
     2221 tttcgcgctt cagggcttcg agatgggctt cggttgcaga agccgcggcg cgttccatgg
     2281 cccgatagcg cgagataagg gccagtccca gcggcgtgag tgtggcgccg ccgcccttct
     2341 tgccgccgct cttggattcg accaccggtt tgccaaacgt cttgttcaga tcctcgatca
     2401 gctcccatgc ccgtcggtag gacatgcgca gtgcccggcc cccagcagca atagagccca
     2461 gcgccgcaat ctcctcgaga agctggatct tgccgggacc gattcgcagg cccgggccaa
     2521 aatcgattcg aatactcaat tgcgccatgc aaagcacctc tgggtgcaat gatcgtccaa
     2581 agtgatccac aaggaaagag cttatcaagc aaccgggatt tcgaacaaga acaaggagtt
     2641 cctgcaatac ctgacgacga cctg
//
LOCUS       DBNGVR010000343         5613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5233168, whole genome shotgun sequence.
ACCESSION   DBNGVR010000343 DBNGVR010000000
VERSION     DBNGVR010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5233168"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="AC16A0_07205"
     CDS             complement(<1..622)
                     /locus_tag="AC16A0_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016163.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HIR3105157.1"
                     /translation="MTPVMGVDGCPGGWIAVIWGAAPMHRLVSRFSDLLDVEAAVIAV
                     DMPIGLPESHGRPPEREVRRRLGERQSSVFAVPARPAVMSEDYREACAINLQHSDPPR
                     KIAKQSFHLFPKIREIDAIITPGLQSRVFEVHPELAFWAMNGEQPLSLPKKTKGRPDP
                     AGQALRRDLLRRAGFPLDLLADATYRQRDVGADDLIDACACAWSARR"
     gene            complement(619..1404)
                     /locus_tag="AC16A0_07210"
     CDS             complement(619..1404)
                     /locus_tag="AC16A0_07210"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR3105158.1"
                     /translation="MRILCISSQVAYGPVGNTAAVPALQAKGHEVLALPTILLSNHPG
                     HGTPHALRVPAHDLAATLGVLEQMGALSTCGAVMTGYFASNDQIHGVARIIRRLKDLN
                     DRLLVLVDPVMGDHDALYVPLPVAKAIRDTLVPLATCVTPNRFELEWLTGWRAGSIKE
                     AEAAARQLNVPDVVATSIPSGSDRLATLSISAAGTIRHLSRLHQQVPHGTGDFLAGLY
                     LARLASGETPSVALPGAMAILDRAVAANAGSDVLDVAAALRAA"
     gene            complement(1408..2580)
                     /locus_tag="AC16A0_07215"
     CDS             complement(1408..2580)
                     /locus_tag="AC16A0_07215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3105159.1"
                     /translation="MKQFDVVIVGGGIVGSATAYYLKKNGFPGGVAIIEKDTTYQHAC
                     TALSAGGIRQQFSTPENIALSTFGLNVVRKLKEEFGADADVNFREQGYLILASGEGLP
                     VLEENHAVQIANGAKNQLLRGDALARRFPYMATEDLAAGCFGYEGEGWVDPYSFMTVF
                     RKAAMARGVDVISGEVTGISVAGGRVASVTLGSGETIGCGRLVNAGGAGAGRLAALAG
                     IDLPVGPRKRYTYVIDCPAASEELHKAPLTVDPVGFYFRPEGRHFISGLSPEEHEEPT
                     EMNWDVDYSWFEERIWPYLAQRVPAFESLKVINAWTGHYDYNALDQNAVIGPHPDIGN
                     FYFANGFSGHGLQQGPAAGNAIAELIIHGAFRTIDLARFGYDRIQRKAPLFEKNVI"
     gene            2692..3558
                     /locus_tag="AC16A0_07220"
     CDS             2692..3558
                     /locus_tag="AC16A0_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3105160.1"
                     /translation="MTIRPRRSVLYMPGANARALEKARTLDADALILDLEDSVAPDAK
                     SEARSRVSEAVKQGGYGHRELVIRINGLDTPWGVDDLRMAAEADADAVLVPKVARPGD
                     IVNVSRIHMALGGAERTRMWAMMETPMAIFNVREIASVHQVARLSCLVLGTNDLLKES
                     RALATGGRFAILPWLSMTLLAARTYGLDVIDGVYNDFRDEAGFRAECEQGRTLGMDGK
                     TLIHPGQIAVCNELFSPAAEEIAWSRRIIAAFQQPENVSKGVITIDGKMVERLHLAMA
                     ERVAAIAEATGQ"
     gene            3555..4604
                     /locus_tag="AC16A0_07225"
     CDS             3555..4604
                     /locus_tag="AC16A0_07225"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3105161.1"
                     /translation="MSKTNPGNFFEDFRLGQVIRHATPRTITVGDQSLYTALYGSRFV
                     VQSSDAFARMAGYPMAPVDDLLLFHVVFGKTVPDISLNAVANLGYADCRFLRAAYPGD
                     TLSTVSEVIGLKENSNGKTGTVYVRSTGRNQRGEEVLTYVRWVMVRKRDEAALAPVAH
                     VPDLPASVAVEHLGQACPPLTGKSWDFDLSGSPHRFADYAVGEKIDHVDGMTVEEAEH
                     QLATRLYQNTAKVHFNQFSESQGRFGRRLIYGGHVISLARALSFNGLANAFHIAAING
                     GRHVAPLFAGGTVYAWSEILDKAGLPGRGDVGALRIMTRATRDLPCAAYPTAAASPEP
                     PRVILELDYWALIPI"
     gene            4725..5600
                     /locus_tag="AC16A0_07230"
     CDS             4725..5600
                     /locus_tag="AC16A0_07230"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HIR3105162.1"
                     /translation="MKIRKVGVIGAGQMGNGIAQVCAQAGYDVGLNDVSKDRIEAGLA
                     TINGNLARRAKSGKISEADRDATLSRISPVVNYDGFGDYDLVIESAVEDEAIKRKIYQ
                     SLCPHLKADCLVGTNTSSISITRLAAATDRPEKFMGIHFMNPVPVMELVELIRGIATD
                     ETTFSTAKAFAVSLNKTVAVAEDFPAFIVNRILMPMINEAVYTLYEGVGSVKSIDESL
                     KLGANHPMGPLELADFIGLDTCLAIMNVLHDGLADTKYRPCPLLTKYVEAGWLGRKTQ
                     RGFYDYRGPTPIPTR"
BASE COUNT         1004 a         1828 c         1783 g          998 t
ORIGIN      
        1 tgcggcgcgc cgaccaggcg caggcacagg catcgatgag gtcgtccgcg cccacgtcac
       61 gctgccggta ggtggcgtcg gccagaaggt ccagcggaaa tccggcgcgg cgcagcaggt
      121 cgcgtcgcag cgcctggccg gcgggatcag gccggccctt cgtcttcttg ggcagcgaca
      181 atggttgctc gccattcatc gcccagaagg ccagttccgg atgcacctcg aacacgcgcg
      241 attgcagacc gggcgtgata atagcatcga tctcgcgaat cttcggaaac aggtgaaagc
      301 tctgcttggc gatctttcgc ggcggatcgg agtgctgcag gttgatggcg caggcctccc
      361 ggtagtcctc gctcatgacg gccggacgcg ccggaaccgc gaagacgctt gactgtcgtt
      421 caccgagcct gcggcgcacc tcgcgttcgg gcggacgccc gtggctttcc ggcaggccga
      481 tcggcatgtc gacagcaatc acggcagctt cgacgtcaag caggtcgctg aatcggctga
      541 ccaggcgatg catgggcgcg gcgccccaga tcacggcaat ccagccgcca ggacaaccat
      601 cgacgcccat gaccggcgtc atgcggcccg cagtgccgcg gccacatcca gcacgtcgga
      661 tccggcgttg gcggcaacgg cccgatccag gatggccatg gcgcccggca gtgccaccga
      721 cggtgtctcg cccgacgcga gacgggcgag atagagcccc gccaggaagt cgcccgtccc
      781 atgcggcacc tgctggtgca ggcgcgagag atggcggatg gtcccagccg cgctgattga
      841 aagcgtcgcc agccggtcgc ttcccgaagg gatcgacgtc gccaccacat ccggcacatt
      901 gagttgccgc gcggccgctt cggcttcctt gatgctgccg gcccgccatc cggtcagcca
      961 ttccagctcg aaacggttgg gcgtcacgca ggtcgcaagc ggcaccagcg tatcgcggat
     1021 cgcttttgcg acgggcaggg gcacataaag cgcatcgtgg tcgcccatca ccggatcgac
     1081 aagcacgagg aggcggtcat tgaggtcctt cagtcgccgg atgatgcggg ctaccccatg
     1141 gatctggtca ttggacgcga agtagccggt catcaccgcc ccgcaggtcg agagcgcccc
     1201 catctgctcc agaacgccca gcgtggccgc caggtcatgt gcgggcacgc ggagcgcatg
     1261 cggcgtaccg tggccgggat ggttggacag caggatggtg ggcagcgcca gaacctcgtg
     1321 ccccttcgcc tgcaatgccg gcaccgctgc ggtattgccc accgggccat aggcaacctg
     1381 cgatgagatg cagaggatgc gcatgtctca gatgacgttc ttctcgaaga ggggagcctt
     1441 gcgctggata cggtcatagc cgaagcgcgc caggtcgatg gtgcggaacg cgccatggat
     1501 gatgagttcg gcgatggcat ttccggcggc cggtccctgc tgcaggccat gccccgaaaa
     1561 gccattggcg aagtagaaat tgccgatgtc gggatgcggt ccgatcaccg cgttctggtc
     1621 gagcgcgttg tagtcatagt ggccggtcca ggcattgatc accttcagcg actcgaaggc
     1681 cggcacgcgc tgggcaagat agggccagat ccgctcctcg aaccaggaat agtcgacgtc
     1741 ccagttcatc tcggtgggtt cttcatgttc ctccggcgag agtccgctga tgaaatgccg
     1801 cccttcggga cggaagtaga agccaaccgg atcgacggtc agcggcgcct tgtgcagctc
     1861 ttcgctggct gccgggcagt cgatcacgta ggtgtagcgc ttgcgcggac ccacgggcag
     1921 gtcgatgccg gcgagtgccg ccagccggcc tgcgccagcg ccgccggcat tgaccagcct
     1981 gccgcagcca atggtttcgc cactgccaag cgtgactgaa gcgacgcgcc cgcccgccac
     2041 gctgatgccg gtgacctctc cggaaatcac gtccacgccg cgcgccatgg ccgctttccg
     2101 gaacaccgtc atgaaggaat aggggtcaac ccagccttcg ccttcatagc cgaagcaacc
     2161 tgcggcgaga tcctctgtcg ccatgtaggg gaatcgccgg gccagggcgt cgccgcgcag
     2221 cagctggttc ttcgcgccgt tggcgatctg gacggcgtgg ttctcctcga gaaccggcag
     2281 gccttcgccc gaggccagga tcaggtaacc ctgttccctg aagttgacat ctgcatctgc
     2341 accgaactcc tccttcagct tgcgaacgac gttcagcccg aaggtcgaca gcgcaatgtt
     2401 ctccggggtc gagaactgct ggcggatgcc accggccgac agcgcggtac aggcatgctg
     2461 gtaggtcgtg tccttttcga tgatggcaac gccccccgga aatccattct tcttcaggta
     2521 gtaggccgtg gcgctaccca cgatcccgcc gcccacgatg accacgtcaa actgcttcat
     2581 gatgtcccaa tctgccccaa acccttgtgc tgccacaagc acttatccaa aagtgggctt
     2641 ccattgctgc agcgcacaag ctagcctgac cggagagtga aggaaacccg aatgacgatc
     2701 cgaccccgac gcagcgtcct ttacatgccg ggcgcgaatg cccgcgcgct ggagaaggcc
     2761 aggacactcg atgccgacgc cctgatcctc gatctggagg attcggttgc gcccgacgca
     2821 aagtccgaag cccgcagccg cgtgtccgag gcggtgaagc agggcggcta cggccatcgg
     2881 gaactggtga tccgcatcaa cgggctggat acgccctggg gcgtggacga cctgcgcatg
     2941 gccgccgagg ccgatgccga tgcggtgctt gttccgaagg tggcgcggcc tggcgacatc
     3001 gtgaacgtgt cgcgcatcca catggcgttg ggcggggccg agcgcacccg catgtgggcc
     3061 atgatggaaa cccccatggc gatcttcaac gtgcgcgaga ttgcctccgt gcatcaggtc
     3121 gcgcggctgt cctgcctggt tcttggcacc aacgatctcc tgaaggaatc ccgcgcgctg
     3181 gccaccggcg gccgcttcgc catcctgccg tggctgtcga tgacgctgct tgcggcgcgg
     3241 acctatgggc tggatgtcat cgacggcgtc tacaacgact tccgcgacga ggcgggtttc
     3301 cgcgccgagt gcgagcaggg gcgcacgctg ggcatggatg gcaagacgct catccatccc
     3361 ggtcagatcg ccgtgtgcaa cgaactcttc tcgccggcag cggaggagat cgcctggtca
     3421 cgccgcatca tcgcggcgtt ccagcagccg gagaacgtgt cgaagggcgt catcaccatt
     3481 gacggcaaga tggtggagcg cttgcatctc gccatggccg agcgcgttgc ggcgatcgcc
     3541 gaggccaccg gtcaatgagc aagaccaatc cgggtaactt cttcgaggac ttccgcctcg
     3601 gacaggtcat ccgccatgcc acgccacgca ccatcacggt gggcgatcag tcgctctaca
     3661 cggcactgta cggatcgcgc ttcgtggtgc agtcttccga cgcctttgcc cgcatggccg
     3721 gctatcccat ggccccggtc gatgacctgc tgctgtttca tgttgtcttc ggcaagaccg
     3781 ttcccgatat ctcgctcaat gccgtggcca atctcggcta tgccgattgc cggttcctgc
     3841 gcgcggccta cccaggcgac acgctctcga cggtatccga ggtcatcggc ctcaaggaaa
     3901 actccaacgg aaagacgggc accgtctatg tgcgctcgac gggccgcaac cagcgtggcg
     3961 aagaggtgct gacctatgtg cgctgggtca tggtccgcaa gcgcgacgaa gcggcgctgg
     4021 caccagttgc gcatgttccc gacctgccgg cttccgtcgc cgtggagcat ctgggtcagg
     4081 cctgtccgcc gctcaccggc aagtcctggg acttcgacct ctcgggatcg ccgcaccgct
     4141 tcgccgacta tgcggtcgga gagaagatcg accacgtcga tggcatgacg gtcgaggagg
     4201 ccgagcatca gctggcaacc cggctctacc agaacaccgc gaaggtgcat ttcaatcagt
     4261 tctcggagtc gcagggccgc ttcgggcgcc ggctgatcta tggcgggcat gtgatttcgc
     4321 tggcccgggc gctctccttc aacggtctcg ccaatgcctt tcacattgcg gccatcaatg
     4381 gcggccggca cgtggccccg ctctttgccg gtggcacggt ctatgcgtgg tccgaaatcc
     4441 tggacaaggc aggcttgccg ggccgcggcg atgtcggagc gctgcgcatc atgacccggg
     4501 ccacccggga tcttccttgc gcagcgtacc ccacggccgc ggcatcgccc gagccgcccc
     4561 gcgtcattct cgagctcgac tactgggccc tgatccccat ctgaagcatg ccgttacctg
     4621 cccccgggca tgcgcaaagt cattgctgca catgcgaaat gcacttgctc cggctgaatg
     4681 cccgttatag gcttcgccgt ctttaactcg gacactcgat taccatgaag atcaggaaag
     4741 tcggcgtgat tggcgccggt caaatgggca acggcattgc ccaggtctgc gcccaggctg
     4801 gatatgacgt cggcctcaat gacgtcagca aggaccgcat cgaagcgggc cttgccacga
     4861 tcaatggcaa tctcgcccgc cgtgcgaaat ccggaaagat cagcgaggcc gaccgtgatg
     4921 ccacgctgtc gcgcatctcc ccggtggtga actacgacgg tttcggcgac tatgacctgg
     4981 tgatcgagag cgctgtcgag gacgaggcga tcaagcgcaa gatctaccag tcgctctgcc
     5041 cgcatctgaa ggcggactgc ctggtgggca ccaatacgtc atcgatctcg atcacgcgcc
     5101 ttgcggcggc gacggaccgg cccgagaagt tcatgggtat ccatttcatg aatccggtcc
     5161 cggtcatgga actggtggaa ctcatccgcg gcatcgcgac cgacgagacc accttcagca
     5221 ccgccaaggc ctttgcggtt tcgctcaaca agaccgtggc ggtggccgag gatttcccgg
     5281 ccttcatcgt caaccgcatt ctgatgccga tgatcaacga ggcggtttat acgctgtacg
     5341 aaggcgtggg ctcggtgaag tcgatcgacg agtcgctgaa gctgggcgcc aaccacccga
     5401 tgggcccgct ggagctggcc gacttcatcg ggctggatac ctgccttgcc atcatgaacg
     5461 tgttgcacga cgggctggcc gataccaaat accggccctg cccgctgctg accaaatacg
     5521 tcgaggccgg gtggctgggc cgtaaaaccc agcgcggttt ctatgattac cgggggccaa
     5581 ccccgattcc gacgcgttaa ccgggcttcc gtt
//
LOCUS       DBNGVR010000344         3609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2680615, whole genome shotgun sequence.
ACCESSION   DBNGVR010000344 DBNGVR010000000
VERSION     DBNGVR010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2680615"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="AC16A0_07235"
     CDS             <1..238
                     /locus_tag="AC16A0_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105163.1"
                     /translation="LRDGETHRSDVGCIADGYQFRCGDDFRLRRRDGESAWAVAGSYD
                     DPENPGVRMLGIELGSDDKLFRLDASESADCSIE"
     gene            303..1328
                     /locus_tag="AC16A0_07240"
     CDS             303..1328
                     /locus_tag="AC16A0_07240"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105164.1"
                     /translation="MTQSQSVIEFRNVTKRFGEVAAVVDNTLAVKSGEFLSILGPSGC
                     GKTTSLRMMAGFEQPTSGEVFIRGENVTGVPAYRRPVNMVFQHYALFPHLSVADNVSY
                     GLRQRSPRPSRREIDSAVDAALAMVRLAGYGMRRTYELSGGQQQRVALARALINRPAV
                     LLLDEPLAALDRKLRSDMQIELQTLQRDVGITFLLVTHDQEEALSMSDRVCVMREGRI
                     VQSGSPRDLYDRPVNRYVADFVGKSNFFAGPTSAEILSVRPEMIEIAATSAKLPKGLS
                     EKLEVRVLNRIFLGEHTEYRVANDRLGEFMVMAPRMSERDAGAFEIGDVLAAGWRKDA
                     ERLLPNE"
     gene            1353..2498
                     /locus_tag="AC16A0_07245"
     CDS             1353..2498
                     /locus_tag="AC16A0_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105165.1"
                     /translation="MTKDHISTQKFLEELRRYQKGSVTRRHFLGVTGLGLATAVMSSA
                     VPGLRPRKAYAELSGTVNFTTWPSYFAQENLDNFTKNTGVKINVAVFGSNEEMLAKLQ
                     AGSTGWDVFVPTNYAISTYKDLGLIEPLDLKRLPNYDPSTQEQRFTAEGSIDGVVYAV
                     PKDWGTTGFVYNSKAMPQKPTSWKEFWDLVQNEATGRAMVHDYQLTTIGNALKYYGYS
                     FNSNDEKELADAEKLLIASKPHLFGINSDYKPSLISGDAWMSVCWTGDAVQLQRDMPE
                     MVYVLAKEGGEIWSDFYAIPKGAPNPDAAYALIDYLLTPAINAKEVEAHGYPSTDSRT
                     NQLVPKAMLENPVLYPAAELLAPLEFGAASTLTNPLRAEIMARFKSA"
     gene            2665..3528
                     /locus_tag="AC16A0_07250"
     CDS             2665..3528
                     /locus_tag="AC16A0_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105166.1"
                     /translation="MNAAPARSRAVTALLVAPAAVFFVFLLIVPLMVVLVFSFGERAP
                     AGGYQPAFTFDNYLNLAARGKAFLNTLTLAPLGTLLTAIIAFPMAYFLAVKVDPKWRV
                     TLLVLVMAPFWTSQLLRYYAWIMLLGAKGIPSWFAYIGITDLRLINTPTAVLIGAVYG
                     FLPLMVPPIYVSLEKLDKRLLEASADLGATPMRTFFQVTLPLALPGVATGSMLVFILL
                     MGDYILPQYLGGGKVFFIGNALVDLFLQSRNWPFGAAVSVALVAIMFVTIFIYMRFVL
                     GKTDRRRAALV"
BASE COUNT          617 a         1219 c         1103 g          670 t
ORIGIN      
        1 gctgcgcgat ggcgagacgc atcgcagcga tgtcggctgc atcgccgatg gctaccagtt
       61 ccgctgcggc gacgacttcc gcctgcggcg gcgtgacggc gagtcggcct gggcggtggc
      121 gggaagctat gatgatcccg agaatcctgg cgtcaggatg ctgggtatag aactcggcag
      181 cgatgacaag ctcttccgcc tcgatgcaag cgaatccgca gattgctcca tcgagtgacg
      241 gccgcgattg acaagcccgc gctgctgatg gaacgtaaac cgtcaattgt atcggcagta
      301 gtgtgacgca aagtcagagc gttatcgagt ttcgcaacgt caccaagcgc ttcggcgagg
      361 ttgcagccgt ggtcgacaac acgcttgcgg tgaaatccgg cgaattcctc tccattctcg
      421 gcccgtccgg ctgcggcaag acgaccagtc tgcgcatgat ggcgggcttc gagcagccca
      481 cctccggcga ggtcttcatc cgcggcgaga atgtcaccgg cgttccggcc tatcgccgcc
      541 cggtcaacat ggtgttccag cactacgcgc tgtttccgca cctctcggtg gcggacaatg
      601 tctcctatgg cctgcgccag cgcagcccgc gcccgtcgcg ccgcgagatc gactccgcgg
      661 tcgatgcggc gctggccatg gtgcgccttg ccggctacgg catgcggcgg acctatgagc
      721 tctccggcgg ccagcagcag cgcgtggcgc tggcccgcgc gctcatcaac cggccggccg
      781 tgctgctgct ggacgagccg ctggccgcgc tcgaccgcaa gctgcgcagc gacatgcaga
      841 tcgagctgca gacactccag cgcgacgtcg gcatcacctt tctcctcgtc acgcatgacc
      901 aggaagaggc gctgtccatg tccgaccggg tctgcgtcat gcgcgagggc cgcatcgtgc
      961 agagcggcag cccgcgcgac ctctatgatc gcccggtcaa ccgctatgtc gccgacttcg
     1021 tcggcaagtc caatttcttc gccggcccca cgagcgccga aatcctgagc gtgcggcccg
     1081 agatgatcga gatcgcggcc acctccgcga aactgccgaa gggcctttcg gagaagctcg
     1141 aggtcagggt gctgaaccgc atcttcctcg gcgagcatac ggagtatcgc gttgccaatg
     1201 accggcttgg tgagttcatg gtcatggcgc cccgcatgtc ggaacgcgat gccggtgcct
     1261 tcgaaatcgg cgacgtgctt gcggcaggct ggcgcaagga tgccgagcgc ctgctgccga
     1321 atgagtaacc aagaacaaca gggagaagac aaatgaccaa ggaccatatc tcgacgcaga
     1381 agtttcttga ggagctgcgg cgctatcaga agggcagcgt cacccgccgc cacttcctcg
     1441 gcgtgacggg cctcggcctc gccacggcgg tgatgagcag cgcggtgccg ggcctcaggc
     1501 cgcgcaaggc ctatgccgaa ctctcgggca cggtgaactt caccacctgg cccagctact
     1561 tcgcgcagga aaacctcgac aacttcacca agaacaccgg ggtcaagatc aacgtcgcgg
     1621 tcttcggctc gaacgaggag atgctggcca agctgcaggc gggttctacc ggctgggatg
     1681 tcttcgtgcc caccaactac gcgatctcga cctacaagga cctgggcctc atcgagccgc
     1741 tcgacctcaa gcgcctgccc aactacgatc cctccacgca ggagcagcgc ttcacggccg
     1801 aggggtccat cgacggcgtg gtctatgccg tgcccaagga ctggggcacc accggctttg
     1861 tctacaattc caaggccatg ccgcagaagc ccacgagctg gaaggagttc tgggatctgg
     1921 tgcagaacga agcgacgggc cgcgccatgg tgcatgacta ccagctcacc accatcggca
     1981 atgcgctgaa gtactacggc tactccttca actccaatga cgagaaggaa ctcgccgacg
     2041 ccgagaagct tttgattgcc agcaagccgc atctcttcgg catcaactcc gactacaagc
     2101 cttcgctgat ctcgggcgat gcctggatga gcgtgtgctg gacgggcgat gcggtgcagc
     2161 tgcagcgcga catgccggag atggtctatg tgctggccaa ggagggcggc gagatctgga
     2221 gcgacttcta tgccatcccc aagggcgcgc ccaatccgga cgccgcctat gcgctgatcg
     2281 attacctgct gacgccggcg atcaacgcca aggaggttga agcccacggc tatccgtcga
     2341 ccgactcgcg caccaaccag ctggtcccca aggcaatgct cgagaacccg gttctctatc
     2401 cggcggccga actgctggcg ccgctcgagt tcggcgcggc ctccacgctc accaatccgc
     2461 tgcgcgccga gatcatggcc cgcttcaaat cggcctgagt gccaagtgtt gccggccctt
     2521 gccctccgtc ctgcggcgag ggcaggggcc ggcgcttcct tggaataact tcatgcggca
     2581 tctgcctgca ctgcgaccgt cgtccatatg cgttgtccat gagcgcgagg aacactgctc
     2641 ccgcggcacc gcgccgaggg ccgcgtgaac gccgcgcccg cccgctcgcg cgccgtgacc
     2701 gcgcttctcg tggcgccggc ggccgtgttc ttcgtctttc tgctgatcgt gccgctgatg
     2761 gtggtgctgg tcttttcctt cggcgaacgc gcaccggcgg gcggctacca gcctgccttc
     2821 accttcgaca actacctgaa cctcgccgcg cgcggcaaag ccttcctcaa cacgctgacg
     2881 ctggcgccgc tcggcacctt gctcaccgcc atcatcgcct tcccgatggc ctacttcctg
     2941 gcggttaagg tcgatccgaa atggcgcgtg acgctgctgg tgctggtcat ggcgccgttc
     3001 tggaccagcc agctcctgcg ctactatgcc tggatcatgc tgctcggcgc caagggcatt
     3061 ccctcctggt tcgcctatat cggcatcacg gacctgcggc tcatcaacac gccgacggcg
     3121 gtgctgatcg gcgcggtcta cggtttcctg ccgctcatgg tgccgcccat ctatgtctcg
     3181 ctggaaaagc tcgacaagcg gctgctcgaa gcctccgccg atctcggcgc cacgccgatg
     3241 cgcaccttct tccaggtgac gctgccgctg gcgcttcccg gcgtcgcaac cggctcgatg
     3301 ctggtcttca tcctgctgat gggcgactac atcctgccgc agtatctcgg cggcggaaag
     3361 gtcttcttca tcggcaacgc cctggtcgac ctgttcctgc agtcgcgcaa ctggcccttc
     3421 ggggcagccg tctcggtggc gctggtcgcc atcatgttcg tgaccatctt catctacatg
     3481 cgcttcgtgc tgggcaagac cgacaggcgg cgggcggcac tggtatgagc atgcgcgtct
     3541 atgccgtcct catctatgtc ttcctctatg cgcctatcgc gctgatcgtc gccggctcct
     3601 tcaatgccg
//
LOCUS       DBNGVR010000345         4253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3662889, whole genome shotgun sequence.
ACCESSION   DBNGVR010000345 DBNGVR010000000
VERSION     DBNGVR010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3662889"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="AC16A0_07255"
     CDS             complement(<1..616)
                     /locus_tag="AC16A0_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ImuA family protein"
                     /protein_id="HIR3105167.1"
                     /translation="MRLETPHARGGGFAVPESRRKDEVLPLVEALRRHIDRLEQGQMR
                     LDRRRSRGTAWTTGLAGIDAHLPAQGLARAGLHDVTPRTYGDMPAAMGFALALAVRRL
                     ADPAERRPLLWCRLARSEREYGRLYGHGLERLGLPRRRFVTVTLDKPAALLWTMEEAL
                     KSGALALVIGDAEPAQAGLTATRRLMLAAQAGKSSGVLIFARAEA"
     gene            747..1205
                     /locus_tag="AC16A0_07260"
     CDS             747..1205
                     /locus_tag="AC16A0_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105168.1"
                     /translation="MKPATPQQAPSRPVLIAATLAMLAMGTLMLLHGLGLYDARWFNP
                     NPLTPAWVFATLGTVLILGALLAAGNIRPLPRTVTQAIGYSLLALCLLVAHWLIFFAE
                     GGSCSLSVVGFGAAVSSLLCRGIMGLALIVFDLILLLALFGTSRRSQPGA"
     gene            1213..1632
                     /locus_tag="AC16A0_07265"
     CDS             1213..1632
                     /locus_tag="AC16A0_07265"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR3105169.1"
                     /translation="MRRRLLIAGLLALPRLVRAESGTQGTIIIGPAWALPADHGDGQL
                     FVPLLNRSAKPDALIAARSTICPLVELRRNNRYDDPPLDRIALEPGQPVPMQPTDRHL
                     RLVSLRQPLRIGESFGVILDFQVAGEIEIAASVEAAP"
     gene            complement(1670..2923)
                     /locus_tag="AC16A0_07270"
     CDS             complement(1670..2923)
                     /locus_tag="AC16A0_07270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3105170.1"
                     /translation="MVRLQGAATLEFPFASRVIDLPLGSAHADLALAIGMQICLNEGG
                     DSVGAIRALAAPDLDVASECLQVLHERPVDAIFLATEDSAKDAVFVTGRAAFAVSFWG
                     ALADGLATQRQDILAALLSLAGRRVLRHAPHQTGTRFEPLAGDGMAKLVTHAEQLGRK
                     LGLLVDKARKPEAARVHRVAVVTPYYREPDSELQRCMASVQGQTMACDHIVVSDGFPN
                     PLVERFAATHVKLGQPHRDNGNTPRYVGGLIAFARGYDSVAYLDADNWYHPRHVELLV
                     RKQHATRATAVCSARNIYLANGLMLPDVDNEDRMRRHVDTSCLMITQSCEFIAHLWGQ
                     MPQSWGPICDRVVFSALNGQSLAWTENRTLNFKSNYAHHYREANQPVPETVHGIPAAL
                     LKSFVLKDRAFQELSRSRTGRIMGM"
     gene            2909..3196
                     /locus_tag="AC16A0_07275"
     CDS             2909..3196
                     /locus_tag="AC16A0_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105171.1"
                     /translation="MKTDHDELQAGVPAKQPGNSITISIRDRILRDEDKGIGAGHACR
                     FSFHVEGVKLPRSCQKAASTDQTPLNCYLVDLGTAALRVTLLVQKKSTPRR"
     gene            complement(3325..>4253)
                     /locus_tag="AC16A0_07280"
     CDS             complement(3325..>4253)
                     /locus_tag="AC16A0_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3105172.1"
                     /translation="IEAGVKAYKAGRHDGVIAFGGGSGLDVGKMIALMHDQNLSVFEL
                     EDIGDWWTRADATKIAPIIAVPTTAGTGSEVGRAGVVTHPITHEKKIIFHPAIMPRVA
                     ILDPELTVGLPPKLTAATGMDALAHNLEAYCAPFYHPLSAGVALEGMRLVKENLAKAV
                     KKGKDIDARGHMLVASAMGATAFQRGLGAIHALSHPFGGLYDAHHGLLNGIVMPYVVK
                     ANRRKIEKDIERAAAYLGIKGGFNGFLKWILALRKEIGIPHKLADIGIDTKRLDEVAA
                     MAIKDPSAGGNPIAFTEKQYKALARKCVVGDL"
BASE COUNT          734 a         1453 c         1317 g          749 t
ORIGIN      
        1 ccgcctcggc gcgggcgaag atgagaaccc cggaggactt gcccgcctgg gccgccagca
       61 tcagccgccg ggtggcggtg agaccggcct gggccggctc ggcatcgccg atcaccagcg
      121 ccagcgcacc ggatttcaga gcctcctcca tggtccagag cagggctgcc ggcttgtcga
      181 gcgtcaccgt cacgaagcgg cggcgcggca ggcccagccg ctccagccca tggccgtaga
      241 gcctcccgta ttcgcgttcg ctgcgcgcca gccggcacca cagcaggggc cgccgctcgg
      301 ccgggtcggc gaggcggcgg accgccagcg ccagggcaaa gcccatggcc gccggcatgt
      361 cgccataggt ccggggcgtc acgtcatgga gcccggcccg ggccaggccc tgggccggca
      421 gatgcgcgtc gatgccggca agacccgtgg tccaggcggt gccccggctg cgcctccggt
      481 caaggcgcat ctggccctgc tccagccggt cgatatggcg gcgcaaggcc tccaccagcg
      541 gcagaacctc atctttccgg cggctttcgg gaacggcaaa gcccccgccc cgcgcgtgcg
      601 gcgtttccag acgcatgact gcaatatcct gaatgttcct attttgttct cacatgtgaa
      661 aagaagagtc aagctaggaa aaattgcttg cgaagaccaa ggcccgattc attctttcct
      721 aatcggcaga cgcgacactc gcgttcatga agcccgccac accgcaacag gctccgtccc
      781 gtccggtcct catcgccgcc accctggcga tgcttgccat gggaacgctc atgctgctgc
      841 atggtctcgg actctacgac gcccggtggt tcaaccccaa tccgctgacc cccgcatggg
      901 tcttcgccac cctcggaacg gtgctgatcc tcggcgccct gctggccgcc ggcaacatcc
      961 gcccgctgcc ccgcaccgtg acacaggcca tcggctatag cctcctcgcc ctctgcctgc
     1021 tcgtcgcgca ctggctgatt ttcttcgccg agggcggcag ctgcagcctg agcgtggtgg
     1081 gcttcggagc ggcggtctca agcttgctct gccgcggcat catggggctg gcgctcatcg
     1141 tcttcgacct catcctcctt ctggccctct tcggaacgtc gcgccgctcg cagccgggcg
     1201 catgaccacg ccatgcgccg ccgcctcctg atcgccggcc tgctcgcgct gccgcgcctc
     1261 gtcagagcag aatccggcac gcaaggcacc atcatcatcg gtcccgcctg ggccctcccc
     1321 gccgatcatg gtgacgggca gctcttcgtc ccgctcctca atcgcagcgc caagccggat
     1381 gcgttgatcg cggcgcgctc caccatctgc cccctggtcg aactccgccg caacaaccgc
     1441 tacgacgatc cgccgctcga ccgcatcgcg ctggagcccg gacagcccgt gccgatgcag
     1501 cccaccgacc gccacctgcg gctggtgtcg ctgcgccagc cgctccggat cggcgaaagc
     1561 ttcggcgtca tcctcgactt tcaagtggca ggggaaatcg agatcgccgc ctccgttgaa
     1621 gcggcgcctt gagccttgtt cgcgagccag ccacaaacga ccgggtcatc tacatcccca
     1681 tgatgcggcc cgttctgctc ctgctcaatt cctggaaggc ccggtccttc agcacgaatg
     1741 acttcaagag cgccgccgga ataccgtgga cggtttcggg aaccggttga ttggcttccc
     1801 ggtagtgatg ggcgtaattc gacttgaaat tcaatgtccg gttctccgtc caggcaagtg
     1861 actgcccgtt gagcgcagaa aagaccacgc gatcgcagat ggggccccag ctctgcggca
     1921 tctggcccca gagatgggca atgaattcgc agctctgcgt gatcatgagg caactcgtat
     1981 caacatggcg ccgcatgcga tcctcgttgt cgacatcggg cagcatgagt ccattcgcca
     2041 ggtagatgtt ccgtgccgag cagacggccg tcgcgcgtgt cgcgtgctgc ttcctgacca
     2101 gaagctcgac atggcgggga tgataccagt tgtcggcatc gagataggca acgctgtcgt
     2161 aaccccgggc aaaggcaatc agtcctccga cataccgggg cgtattgccg ttgtcgcgat
     2221 gcggttggcc cagcttgacg tgggttgcag caaaacgctc gaccagcgga tttggaaagc
     2281 cgtccgaaac gacaatgtgg tcgcatgcca tggtctgccc ctggacgctc gccatgcagc
     2341 gctggagttc ggaatcaggc tccctgtaat agggcgtgac cactgccacg cgatggacgc
     2401 gggcagcctc cggcttccgc gccttatcga ccaagagtcc cagcttgcgc ccgagctgct
     2461 ccgcatgggt aacgagcttc gccatgccgt cgccggcgag cggctcgaac cttgtgccgg
     2521 tctggtgcgg cgcatgccgc aggacgcggc gcccggcaag actaagcaat gcagcaagga
     2581 tgtcttgccg ctgcgtggca agtccatcgg ccagggcacc ccagaaggag acagcgaatg
     2641 cggcacggcc ggtcacaaag actgcatcct tcgccgaatc ctcggtcgcg agaaagatgg
     2701 catcgaccgg cctctcatgc aggacctgca ggcactccga ggccacgtcg agatccggcg
     2761 ctgcaagcgc cctgatggcg ccgacgctat cgcccccctc gttcaggcag atctgcatgc
     2821 caatcgccag agccaggtcg gcatgcgcgc tccccagcgg caggtcgatc acgcggctgg
     2881 cgaacggaaa ttccagcgtt gctgcgcctt gaagacggac cacgatgaac tgcaagccgg
     2941 cgtgccggcg aagcagcccg gcaacagcat cacgatcagc atccgtgaca ggatcctgcg
     3001 cgacgaagac aagggcatcg gagcggggca cgcgtgtcgt ttctcgttcc atgtcgaggg
     3061 cgtgaagctg ccacgatcat gccagaaagc agccagcact gaccagaccc cgctcaactg
     3121 ttaccttgtt gaccttggca ctgccgcctt gcgggtcact ttgcttgtac agaaaaagtc
     3181 aacacctcgc cgctgacggc gtccttgccg aagggcggga gtcccaccgc gcttcgcgat
     3241 ccggcgctca gggcagaccc tgtggcagag cgatcataac gcctgcggtc gcgggcggct
     3301 ttccggtccg agggaccctc ggtcctaaag atcccccacc acgcatttgc gcgccagcgc
     3361 cttgtactgc ttctcggtga aggcgatggg attgccgccc gccgaaggat ccttgatcgc
     3421 catcgcagcc acctcgtcga ggcgcttggt gtcgatgccg atgtcggcaa gcttgtgcgg
     3481 tatgccgatc tccttgcgca gcgcgaggat ccacttgagg aaaccattga agccgccctt
     3541 gatgccaaga taggcggcag cgcgctcgat gtccttctcg atcttgcggc ggttggcctt
     3601 taccacgtaa ggcatgacga tgccgttgag caggccgtga tgcgcgtcgt agaggccgcc
     3661 gaagggatgc gacagcgcat gaatggcgcc cagtccgcgc tggaaggcgg tggcgcccat
     3721 ggccgaggcc accagcatat ggccgcgggc atcgatgtcc ttgcctttct tcaccgcctt
     3781 ggcgaggttt tccttcacca gccgcatgcc ttccagcgca acgcccgccg aaagaggatg
     3841 gtagaagggc gcgcaatagg cctcgagatt gtgggccagc gcgtccatgc cggtggctgc
     3901 cgtgagcttg ggcggcaggc ccacggtgag ttcgggatcg agaatggcca ccctgggcat
     3961 gatggccgga tgaaagatga tcttcttttc gtgcgtgatg gggtgcgtga ccacgccggc
     4021 acggcccact tccgaacccg tgcccgccgt ggtgggcacc gcaatgatcg gcgcgatctt
     4081 cgtggcatcg gccctggtcc accagtcgcc gatgtcctcc agttcgaaga cggacagatt
     4141 ctggtcatgc atcagcgcga tcatcttgcc gacatcgagg cccgatccgc cgccgaaggc
     4201 gatgacgccg tcatgccgcc cggccttgta ggccttgacc cctgcctcga tgt
//
LOCUS       DBNGVR010000346         6375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5234587, whole genome shotgun sequence.
ACCESSION   DBNGVR010000346 DBNGVR010000000
VERSION     DBNGVR010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5234587"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="AC16A0_07285"
     CDS             complement(<1..1391)
                     /locus_tag="AC16A0_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR3105173.1"
                     /translation="MSGEITARLPPPLDAAAVRRFRDAMPDALRDGPRPDLVDGLAGA
                     SPYLRDLMLADPDFAAEAFVANPESVLDRIIAGLRMVADGTCQTDFMAALRTAKAKAA
                     LLIAIADTGGRWPLAEVTAAITRFADASLQAAVDWLLREAHAAGRLVLADPSMPSQGC
                     GYTVLAMGKQGAFELNYSSDVDLIVLYDPDAAPLAPGVEPSTFFVRFTKRLVSLLQDV
                     TEHGYAFRVDLRLRPDPRATQVAISIEAAAIYYESMGQNWERAAMIKARPAAGDLPLG
                     QEFLDRLKPYVWRKYLDFAAIADVQSLKRQIHAVKGPGTIAVMGHNLKLGRGGIREIE
                     FFVQTQQLIAGGRNPKLRGRGTLDMLAALAEANWITPQALNELREAYRFLRTIEHRVQ
                     MVNDEQTHSLPADNRQFESLARFSGFDSATAFHDRLRTTFECVQRHYAALFDDASALA
                     SESGSLVFTGGED"
     gene            complement(1402..2790)
                     /locus_tag="AC16A0_07290"
     CDS             complement(1402..2790)
                     /locus_tag="AC16A0_07290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3105174.1"
                     /translation="MRAGFKLLRTSTFRLAALYLAVFALSVGALLGYVYWNTAVLLER
                     QTDETIRAEVQALADQYRYRGLRGIIDTIQRRSKDDRGSLYLLSDPQGRRVAGNLETM
                     PAEASSEDPWIEFPLDVERGGRIERHVARAFHTKLTGDFELVVGRDVDALRQFAGIMR
                     VTLFYALAIALVLGLGGGLLMSRNFLRRVDAITAASHTIMAGDMGGRMPVSGSDDELD
                     RLALALNQMLDQIQRLMAGMQEVSSNVAHDLKTPLTRVKARVEAALRSGNAGEYKAAL
                     EKTVEESDRLLDTFNALLSIARAEAGQARSGLEPIDASQVIADVAELYEPTAEELGGN
                     LQFDVAPDLNVRGDRQLLAQALSNLIDNALKYGAREGQGPEIKVTGQVEGNNVVIAVG
                     DHGEGIAPGDRGRVTERFVRLDSSRARPGNGLGLSLVSGVMKLHDGALVLDDNGPGLL
                     AKLVLPLQRAAS"
     gene            complement(3008..3679)
                     /locus_tag="AC16A0_07295"
     CDS             complement(3008..3679)
                     /locus_tag="AC16A0_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3105175.1"
                     /translation="MRILVIEDDKEAANWLIKGLSESGHVADHAADGEQGLNMASEGL
                     YDVLIVDRMLPKLDGLGIIRKLRDDGNRTPALILSALSDVDERVKGLRAGGDDYLGKP
                     YAFSELLARVENLGRRPTEKPFETTLRAADLEIDILSRTVTRAGKPILLQPREFKLLE
                     YLVRNAGHIVTRTMLLENVWDYHFDPQTNVIDVHVSRLRSKIDKGFDEPILQTVRGAG
                     YMIKA"
     gene            complement(3873..5333)
                     /locus_tag="AC16A0_07300"
     CDS             complement(3873..5333)
                     /locus_tag="AC16A0_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3105176.1"
                     /translation="MTNILTRARPRLLASVAAGLMAATAITAAVTALPSTPVRAESAA
                     AIDPTKGFGDLVDRVMPAVISVEVKFTNASASDEQSAAPQMPEGMPEQFRDFFKKFGE
                     GQGQQPMPRGPGMAQGSGFVISADGYAVTNNHVVKDADEVAVKLRDGTELPAEVIGTD
                     PKTDLALIKIKSDKPLATVKFAKTEPRVGDWVMAVGNPFGLGGTVTTGIISARGRDIG
                     AGPYDDFLQIDAAINRGNSGGPAFNLEGEVIGINTAIFSPSGGSVGIGFAIPASTAET
                     IIDSLRETGAVTRGWLGVQIQPVTEDLAEGLGLEEAKGAVISDVTEKSPALDAGLKSG
                     DTILKVDGQEVKDARDLARKVAKVKPGTSVPVTIVRDGKEQEIAVKIGVMPGEDQMAE
                     AKGDKSGADLKDLGLKVVPAEDGAGVTVTEVAPDSAAAERGLRPGDVILEVAGAEVHN
                     PADVRDAMKASGKKRVLMLVKSGDGQRFIALPANAG"
     gene            complement(5438..5884)
                     /locus_tag="AC16A0_07305"
     CDS             complement(5438..5884)
                     /locus_tag="AC16A0_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR3105177.1"
                     /translation="MRRLTGILLLLLMLAPAQALEPQELLPDPALEARARALSAQLRC
                     LVCQNQSIDDSDAPLAKDLRSLIREHLQKGESDAQIMDYVVERYGDFVLLKPRLSAQT
                     LVLWGTPFVVLVIAAGMLLLRRRGQSAAPERPLSESERQALKKALE"
     gene            complement(5881..>6375)
                     /locus_tag="AC16A0_07310"
     CDS             complement(5881..>6375)
                     /locus_tag="AC16A0_07310"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR3105178.1"
                     /translation="VLAHGGLGIMVMGIIAISLWKVEVIVAMKPGETADVAGYAVTFK
                     GETPLTGPNYTGLAGQFDVTRNGQDVARLVSEKRNFKPSGMPTTEVGLHQSLAGDLYV
                     VMGDANPDGGRSMRLYFNPLVNLIWLGAALMFLGGAFSLADRRYRVGAPRPSAAPGKA
                     VAAE"
BASE COUNT         1054 a         2130 c         2108 g         1083 t
ORIGIN      
        1 tcgtcctcgc cgccagtaaa gacgaggctt ccggactcgc tcgccagcgc cgatgcatcg
       61 tcgaacagcg cggcgtagtg gcgctgcaca cattcgaagg tggtgcgcag ccggtcatgg
      121 aacgccgtgg cgctgtcgaa gccgctgaag cgcgccagtg actcgaactg ccggttgtca
      181 gccggcaggg aatgggtctg ttcgtcgttc accatctgca cgcgatgctc gatggtccgc
      241 aggaaccgat aggcctcgcg cagctcattc agggcctgcg gcgtgatcca gttggcctcg
      301 gccagggccg ccagcatgtc gagcgtgccg cggccgcgca gcttcgggtt gcggccgccc
      361 gcgatcaact gctgggtctg cacgaagaac tcgatctcgc ggatgccgcc acggcccagc
      421 ttgagattgt gccccatcac cgcgatggta ccggggccct tcaccgcgtg gatctgccgc
      481 ttcagcgact gcacatcggc aatggcggcg aagtcgagat acttgcgcca cacatagggc
      541 ttcagccgat cgagaaactc ctggccgagc ggcaggtcgc cggcggcagg ccgcgccttg
      601 atcatcgcag cgcgctccca gttctggccc atgctctcgt agtagatcgc ggcggcctcg
      661 atcgagatcg ccacctgcgt ggcgcgcgga tcggggcgca ggcggagatc gacgcgaaag
      721 gcatagccat gttcggtcac gtcctgcagc agcgacacga gccttttggt gaaccgcacg
      781 aaaaaggtgg agggctcgac gcccggcgcc agcggcgcgg catcgggatc gtaaagcacg
      841 atgaggtcga catcgctcga gtagttcaac tcgaaggcac cctgcttgcc catggccagc
      901 accgtgtagc cgcagccctg cgatggcatc gacggatcgg caagcacaag ccggccagcg
      961 gcatgggctt cgcgcagaag ccagtccacc gccgcctgca ggctggcatc cgcgaagcgg
     1021 gtgattgcgg ccgtcacctc cgccagcggc cagcgcccgc ccgtatcggc gatcgcgatc
     1081 agcaaggcag ccttggcctt ggcggtgcgc aaggccgcca tgaagtcggt ttggcaggtc
     1141 ccatccgcca ccatccgcag cccggcgatg atgcggtcca gcacggattc aggatttgca
     1201 acgaaggctt ccgccgcaaa atccggatcc gccagcatca agtcgcgcag atagggcgac
     1261 gcacccgcga ggccatcgac gagatccgga cgcggaccgt cgcgcaatgc atccggcatg
     1321 gcgtcgcgga agcggcgcac ggccgcggcg tcgagcggcg gtggcagccg cgctgtgatc
     1381 tctccggaca tggcggctgg tttagctggc tgcgcgctgc agcggaagca ccagcttggc
     1441 gaggagcccg gggccgttgt cgtccagcac gagcgcgccg tcatgcagct tcatgacgcc
     1501 ggagaccagg ctcagaccca ggccattgcc gggtcgcgca cggctggagt ccagccgcac
     1561 gaagcgctcc gtcacccgtc cacggtcccc gggcgctatg ccttcgccgt gatcgccgac
     1621 cgcgatgacc acgttgttgc cctccacctg gccggtgacc ttgatctccg gcccctgccc
     1681 ttcgcgtgca ccgtatttga gcgcgttgtc gatcagattg ctcagggcct gggccagcag
     1741 ctggcggtcg ccccggacat tcaggtccgg ggctacatcg aactgcagat tgccgccaag
     1801 ctcttccgcc gtgggttcat agagttccgc cacatcggcg atgacctggc tggcgtcgat
     1861 cggttcaagc ccgctccggg cctgacctgc ttcagcgcgg gcaatggaga gcagcgcatt
     1921 gaaggtatcg agcagacggt cggattcctc caccgtcttt tccagcgccg ccttgtactc
     1981 cccggcattg ccagagcgca gcgccgcctc cacccgcgcc ttcacccggg taagcggcgt
     2041 cttgaggtcg tgcgccacgt tgctcgagac ctcctgcatg ccggccatca gacgctggat
     2101 ctggtccagc atctggttca gcgccagggc caggcggtcg agctcgtcgt ccgatccgct
     2161 caccggcatg cgcccgccca tgtcgcccgc catgatggtg tggctggcgg cggtgatggc
     2221 atcgacgcgg cgcaggaagt tgcggctcat cagcaggccg ccgcccaggc ccaggaccag
     2281 cgcgatggcc agggcgtaga acagcgtcac gcgcatgatg cccgcgaatt gccgcagcgc
     2341 atcgacatcg cgccccacca ccagctcgaa gtcaccggtc agcttggtgt ggaaggcccg
     2401 cgccacatgc cgctcgatcc gcccgccgcg ctcgacatcc agcggaaatt cgatccaggg
     2461 atcctcactg ctcgcctcgg ccggcatggt ttcgagattg ccggcaaccc ggcggccctg
     2521 cggatcggag aggaggtaga gcgagccgcg gtcgtccttg ctgcggcgct ggatcgtgtc
     2581 gatgatgccg cggagcccgc ggtagcgata ctgatcggcc agcgcctgca cttccgcgcg
     2641 gatcgtttca tcggtctggc gctccagcag cacggcggtg ttccagtaga catagcccag
     2701 cagcgcgccg accgagagcg cgaagaccgc gaggtagagc gccgcgagcc ggaaggtcga
     2761 tgtccgcagg agcttgaaac ccgcccgcat cgttcaaacg ccctccacat gcttgatcat
     2821 gccacgtcgc ccacgaagcg gagcggcatg acgccgtcca gaatgatccc ggaagtctcg
     2881 catagccgtg tccctgggca aagtgaagaa gcaggcggcg tggcaacggc gaggaccaga
     2941 tgtccctcaa tgccaaaacg gggcgcgccg tcgcaccccg ttccggccgt ccaactttcg
     3001 agaccggtca ggccttgatc atgtagcccg cgccgcgcac ggtctgcagg atcggctcgt
     3061 cgaaaccctt gtcgatcttc gaccgcaggc gcgagacatg cacgtcgatc acattggtct
     3121 gcggatcgaa gtggtagtcc cagacgttct ccagcaacat cgtgcgggtg acgatgtggc
     3181 cggcattgcg gaccagatac tccagcagct tgaactcgcg cggctgcagc aggatgggct
     3241 tgccggcacg cgtcaccgtg cgggacagga tgtcgatttc gagatcggcc gcgcgcagcg
     3301 tcgtctcgaa gggcttctcg gtcggccggc ggcccaggtt ctcgacgcgg gccagcagtt
     3361 cggagaaggc atagggcttg ccgagatagt cgtcgccgcc cgcccgcagg cccttcacgc
     3421 gctcgtccac atcgctgagc gcgctgagga tgagcgccgg ggtgcggttg ccgtcgtcgc
     3481 gcagcttgcg gatgatgcca agaccgtcca gcttgggcag catgcggtcg acgatcaaca
     3541 cgtcatagag gccctcggag gccatgttca ggccctgctc gccatcggcg gcatgatcgg
     3601 ccacatggcc gctctcgctc aggcccttga tcagccagtt cgccgcttcc ttgtcgtctt
     3661 cgatcacgag aatacgcatg gacgggcgaa ctgaacctgt ggcgtcggct gatttggatt
     3721 cgaagtccat tgcttgcatg ttccggggct cctgaaaagg gggcggagac tggatcacct
     3781 ctaggaaccg gatgagggtc cagcctccgc gggacagata cgcgaactcg agcgggggct
     3841 tggggggatg gctcgcgtat cagttactcg tgtcagccgg cgttggccgg cagcgcgatg
     3901 aagcgctggc cgtcgccgct cttcaccagc atcagcaccc gcttcttgcc ggacgccttc
     3961 atggcgtcgc gcacatcggc gggattatgc acttcggcac cggcaacctc gaggatcacg
     4021 tccccggggc gcaggccgcg ctcggcggcc gcgctgtcgg gggcaacctc ggtcacggtg
     4081 acgccagcgc catcttcggc cggcaccacc ttgaggccaa gatccttgag gtccgcgccg
     4141 gacttgtcgc ccttggcctc ggccatctgg tcttcgcccg gcatgacgcc gatcttgacc
     4201 gcgatctcct gctccttgcc gtcgcgcacg atggtgacgg gcaccgacgt cccgggcttc
     4261 accttggcaa ccttgcgggc gaggtcacgg gcgtccttca cctcctggcc atcgaccttg
     4321 aggatggtgt cgccggactt gaggccggcg tccagcgccg gagatttctc ggtcacatcc
     4381 gagatcaccg cacccttggc ttcttcgagc ccgaggcctt cggcgaggtc ctcggtcacc
     4441 ggctggatct gaacgccaag ccagccgcgg gtcaccgcac cggtctcgcg caggctgtcg
     4501 atgatcgtct ccgcggtcga ggccgggatg gcgaagccga tgcccaccga tccgcccgag
     4561 ggcgagaaga tggcggtatt gatgccgatc acctcgcctt ccaggttgaa ggccgggccg
     4621 ccggagttgc cgcgattgat ggccgcgtcg atctgcagga agtcgtcata gggaccggcg
     4681 ccgatgtcgc ggccgcgagc cgagatgatg ccggtggtca cggtgccgcc caggccgaag
     4741 ggattgccca cggccatgac ccagtcgccg acgcgcggct cggtcttggc gaacttcacg
     4801 gtggcgagcg gcttgtccga cttgatcttg atgagggcaa gatcggtctt gggatcggtg
     4861 ccgatcactt cagccgggag ttccgtgccg tcgcgcagct tcaccgccac ctcgtcggcg
     4921 tccttgacga cgtggttgtt ggtgacggca tagccgtcag ccgaaatcac gaatcccgaa
     4981 ccctgggcca ttccggggcc gcgcggcatg ggctgctggc cctggccttc gccgaacttc
     5041 ttgaagaagt cgcggaactg ctcgggcatt ccctccggca tctgcggcgc cgcgctctgt
     5101 tcatccgacg cggatgcgtt ggtgaacttc acttcgacgc tgatcacggc cggcatgacg
     5161 cggtcgacga gatcgccgaa gcccttggtg ggatcgatgg ccgcggccga ttcggcgcgg
     5221 acaggcgtgg atggaagcgc ggtgacagcc gcggtgatcg cggtcgccgc catgagaccg
     5281 gcggcgacgg atgccaggag ccgggggcgg gcgcgagtca ggatgttggt cattcggtcg
     5341 ttctccatca ctggtaagtt tccaatgagg aggaagatag gtccgccacc ttacaatggc
     5401 atttcgacca ggtgaatctt tggtaatgcg gctggcgcta ctccagggcc tttttcagcg
     5461 cctggcgttc ggactcgctg agcgggcgtt ccggcgcggc ggactggcca cggcggcgca
     5521 gcaacaacat gccggcggcg atgaccagca cgacaaaggg tgtgccccag agcaccagcg
     5581 tctgcgcgga aagccgcggc ttcagcagca cgaagtcgcc gtagcgttcc accacgtagt
     5641 ccatgatctg ggcatcgctc tcgcctttct gcagatgctc gcggatgagg ctgcgcaggt
     5701 ccttggccag cggcgcatcg gaatcgtcga tcgactggtt ctggcagaca aggcagcgca
     5761 gttgcgccga cagcgcccgg gcccgcgcct cgagtgccgg gtccggcagc agttcctgcg
     5821 gctccagcgc ctgggccggt gccagcatca acaacagcag caggattccc gtcagccgcc
     5881 tcattccgcc gccactgcct tgccgggtgc ggcactgggg cgcggcgctc cgacgcggta
     5941 tcggcggtcg gccagcgaga aggcgccgcc caggaacatc agcgccgcgc caagccagat
     6001 gagattcacc agcggattga agtagagccg catcgagcgg ccgccatcgg gattggcatc
     6061 gcccatcacc acatagaggt cgccggccag cgactggtgc aggccgactt cggtggtcgg
     6121 catgccggag ggcttgaagt tgcgcttctc cgaaaccagc cgcgccacgt cctgcccgtt
     6181 gcgggtcacg tcgaactggc cggcaagccc ggtgtagttg ggacccgtca gcggcgtctc
     6241 gcccttgaag gtcaccgcat agccggcgac atcggccgtc tcgcccggct tcatcgccac
     6301 gatcacttca accttccaga gcgagatggc gatgatgccc atcaccatga tccccagccc
     6361 gccatgggcc agcac
//
LOCUS       DBNGVR010000347         3045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3074140, whole genome shotgun sequence.
ACCESSION   DBNGVR010000347 DBNGVR010000000
VERSION     DBNGVR010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3074140"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC16A0_07315"
     CDS             <1..272
                     /locus_tag="AC16A0_07315"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3105179.1"
                     /translation="RTVPDGLALDEAGNLYISLYNPNIIYRLSPDGTLATLYNDWEQL
                     MLIAPTNIAFGGPDMRTLIIASLCGWSVHVATVDAPGLRLRYPKM"
     gene            complement(409..1368)
                     /locus_tag="AC16A0_07320"
     CDS             complement(409..1368)
                     /locus_tag="AC16A0_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3105180.1"
                     /translation="MSRNYAIGIDLGGTQVRTALLDIGGTILKRASEPTRAKDGAEVV
                     IDQIVRLVTCVSDGLQQAELAGAGVSSPGPLDTTAGVALGIPTIAGFAGYPMREEIGR
                     RLSLPVALENDGIAAAIGEWKFGAGRGRDNLVYVTVSTGIGGGVIADGRVLRGRRGMA
                     GHVGHMSFVQDGELCVCGGRGCFEAYGSGSALAQRARAAAQDDPSSSLNGSGQISSED
                     VFAAAAKGDRLAQALVQQEAEILGRGFAALLHLYSPEVLVMGGGLSSQFETLEAGIIQ
                     AMRAHAMPAFRDTPVMPAELGSNSGLVGAAVLAFERVRQAGRP"
     gene            complement(1365..2057)
                     /locus_tag="AC16A0_07325"
     CDS             complement(1365..2057)
                     /locus_tag="AC16A0_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3105181.1"
                     /translation="MTDAHWIDALPSHRLICEFSAWSADLLRLADDMQRIASHCDILH
                     VDVADGHFAPALLFFPDLVAALRKASRAPVHTHLMAADEILLDQITQFADAGSDLISI
                     HAENANIDDALDLLEERKIPAGLVLKVDSPLDVLPRYLPRLRFVTLLGTAIGIKGVGL
                     DPRATGRLTAARALIAAAQLPHRVVLASDGGNREQTLPLLRQAGAETVVLGSLAFGAP
                     SLEDRMDWIHGL"
     gene            complement(2054..2869)
                     /locus_tag="AC16A0_07330"
     CDS             complement(2054..2869)
                     /locus_tag="AC16A0_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3105182.1"
                     /translation="MSEDVFRPHDGPRLRVADGGGTGMPVLFQHGLCGDAAQTAEVFP
                     PGVGLRCITLECRGHGGSEAGDVTAFSIVRFADDIAEFISARRLAPLVVGGISMGAAI
                     AMRLAVTQPDLVRGLVIARPAWSILAAPANMRPNAEVGELLSSMRPEAARQAFAASGT
                     AQRLAAESPDNLVSLMGFFSREPSVVTAALLASISRDGPGVDADQLRRIAVPTLVIAT
                     GMDLVHPLTMAGELSRLIPHARLTEITPKGVDRKAYVAEFRDALDDFLKGFLR"
     gene            complement(2875..>3045)
                     /locus_tag="AC16A0_07335"
     CDS             complement(2875..>3045)
                     /locus_tag="AC16A0_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3105183.1"
                     /translation="FAAAGTGVVDFRHFLACLAAAGFDGPLITHGLTAKEAPGVARFL
                     GDTLAGLGLGAS"
BASE COUNT          576 a         1033 c          965 g          471 t
ORIGIN      
        1 cgcgcaccgt gccagacggg cttgcgctgg acgaagccgg caatctctac atcagcctct
       61 acaatcccaa tatcatctac aggctctcgc cggacgggac gctcgccacg ctctacaatg
      121 actgggaaca gctgatgctg atcgccccca cgaacatcgc cttcggcggc cctgacatga
      181 ggaccctgat tatcgccagc ctctgcggct ggtcggtgca tgtcgccact gtcgacgcgc
      241 cgggtctcag attgcgctat ccgaagatgt gaccagcgac tgacggcaaa ggccggaaca
      301 ggctagcagg gaaccgatcc atccgcctgc ccggcggcgt ccacagcgaa ggcacagctg
      361 cgcgcaacat gaacggcccc gcgcctcaca agggggtttt gcagtccttc acggccgccc
      421 ggcttgcctc acccgttcga atgccaggac ggccgctccg acaagcccgg agttgctgcc
      481 gagttccgcc ggcatcacag gcgtatccct gaacgccggc atggcatggg cgcgcattgc
      541 ttgaatgatg cccgcttcca aggtttcgaa ctggctcgac agtccaccgc ccatcaccag
      601 cacctcggga ctgtagaggt ggagaagcgc cgcaaatccc cggccgagga tctcggcctc
      661 ctgctggacg agcgcctgcg ccagacgatc accctttgcg gcagcggcga agacatcttc
      721 ggaactgatc tgaccactgc cattcaggct ggaagacgga tcgtcctggg cagccgcgcg
      781 tgccctttgg gcaagggccg agcctgagcc ataggcttcg aagcaaccgc ggccgccgca
      841 gacacacagt tcgccgtcct gcacgaagct catgtggccg acatgtccgg ccatgccccg
      901 ccgcccgcgc aacacccgac cgtcggcgat cacaccgccg ccgatgccgg tgctcaccgt
      961 gacatagacg aggttgtcgc ggccgcgccc ggcgccgaac ttccattcgc cgatcgccgc
     1021 ggcaatgccg tcattctcca gtgcgaccgg cagggacagt ctgcggccga tttcttcccg
     1081 catcggatag ccagcgaatc ctgcgatggt cggaatgccg agggcgacgc ctgccgtggt
     1141 gtcgagcggt ccgggcgatg acactcccgc gcctgcaagc tcggcctgct gcaggccgtc
     1201 ggacacacag gtgacgagac ggacgatctg atcgatgacc acctcggcgc cgtccttcgc
     1261 gcgagtcggt tccgaagcgc gcttcaagat cgtcccgccg atgtcgagga gagccgtgcg
     1321 gacttgtgtg ccgcccagat cgatgccgat cgcgtagttc ctgctcacaa gccgtgtatc
     1381 cagtccatgc ggtcctcgag gcttggcgcc ccgaaggcca gcgagccgag aacaacggtt
     1441 tcggccccgg cctgccggag cagcggaagc gtctgctcgc gattgccgcc gtccgatgcc
     1501 agaaccacgc ggtgcggcag ttgcgctgcc gcgatcagcg ctctcgcggc cgtcaggcgt
     1561 ccggtggcgc gcggatcgag cccgacgccc ttgatgccga tcgcggtccc aagcaaggtg
     1621 acaaagcgaa ggcgcggcag gtagcgtggc agcacgtcca gcggcgaatc caccttgaga
     1681 accagcccgg ccggaatctt ccgctcttcc agaaggtcga gtgcgtcgtc gatgttggcg
     1741 ttttccgcgt ggatgctgat caggtcgctg ccggcatcgg cgaattgcgt gatctgatcg
     1801 agcaggatct catccgcggc catcaggtgc gtgtggactg gcgctcgcga tgccttgcgc
     1861 agggccgcga cgagatcggg aaagaacagc agcgccgggg caaagtgccc gtccgcaaca
     1921 tccacatgaa ggatgtcgca gtgggaggcg atgcgttgca tgtcgtcggc cagccgcagg
     1981 agatcggccg accatgctga gaactcgcag atcagacggt gagacggaag agcgtcgatc
     2041 cagtgagcgt cggtcatcgc aggaatcctt tcagaaaatc gtcaagcgcg tctcggaact
     2101 cggcaacata cgccttgcga tcgacgccct tcggcgtgat ctcggtcagc cgggcgtgcg
     2161 gaatgaggcg cgacagctct ccggccattg tcagagggtg gaccagatcc atgcccgtcg
     2221 cgatcacaag cgtgggtaca gcgatccgtc gcagttgatc cgcatcgacg cccgggccgt
     2281 cacgcgagat ggaggccaga agggccgccg tcaccaccga cggctcgcgc gagaagaacc
     2341 ccatgaggga cacgagattg tcgggtgact cggcggccaa tcgctgcgct gtgccgctgg
     2401 cggcaaaggc ctggcgcgca gcctcgggcc gcatgctgga cagaagctca cccacttcgg
     2461 cattcggccg catgttggcc ggcgccgcca ggatcgacca ggctggccgc gcgataacga
     2521 gaccgcgcac cagatcgggc tgcgttaccg caagccgcat ggcaatagcc gctcccatcg
     2581 agatgccgcc aaccaccaga ggtgccaagc ggcgcgcgga aatgaactct gcaatgtcat
     2641 cggcaaagcg gacgatggag aatgcggtca catcgccggc ttccgagccg ccatgaccgc
     2701 ggcactccag cgtgatgcat cgcaacccga cgcccggcgg aaagacctcc gccgtctgcg
     2761 ccgcgtctcc gcaaagaccg tgctgaaaga gaacaggcat gcccgttcct cccccatcgg
     2821 caacccgcag tctcggccca tcgtgcggcc ggaagacatc ttcagacatc acggtcatga
     2881 tgcgccgagt ccaaggccgg ccagcgtgtc gcccagaaac ctggcaaccc caggcgcctc
     2941 cttcgccgtt agcccatggg tgatcagcgg gccgtcgaag cccgccgccg caaggcaggc
     3001 aagaaagtgg cggaagtcga cgacacccgt gccggcagcc gcgaa
//
LOCUS       DBNGVR010000348         6718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2683094, whole genome shotgun sequence.
ACCESSION   DBNGVR010000348 DBNGVR010000000
VERSION     DBNGVR010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2683094"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1351
                     /locus_tag="AC16A0_07340"
     CDS             185..1351
                     /locus_tag="AC16A0_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3105184.1"
                     /translation="MAYFLLLFSAVMLAVHLVTQIIVLRRKSSQISSDDPATAEAVTI
                     VRPISQIEPHIWQTLESTFQIDWPNLAIVFCAARESDPAVAIVRALLDRHPDAHAQLL
                     LGESRISDNPKLNNCVKGWTAARTDVVAFVDSNVLLPRDYLKVMMACWNEETGMISVP
                     AVGAAPGSFWAETECAILNTHQARWQLVADTCGMGFAQGKNLMFRKSVLDPLGGIAVL
                     AREPAEDAAATKAIRQASLCVRLAPMPFPQPLGAKTFRQVWNRHLRWAKLRRATFPLQ
                     YLPEFLTGSVLPFTALVSALLILGIDLLVPALVLAAVWFGSELVTARRLGWHVSPWTP
                     VSLAARDLLLPVLWIAGFSPGGFEWQGHAMSAQRSHGGAAEAEHDTDTATTVTT"
     gene            1361..2020
                     /locus_tag="AC16A0_07345"
     CDS             1361..2020
                     /locus_tag="AC16A0_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR3105185.1"
                     /translation="MPALAETPFVHEGAVVRTSQLGRYVEIGAGSRIVESQIGDYSYT
                     DRYADIAYSTLGKFANVAAFSRINPGEHPYHRASLHHFMYRSSYYWPDEADEAAVFDW
                     RRSRPVRIGHDTWIGHGAIIMKGVTVGTGAIVASNAVVTKPVPPYAVVAGTPAKLIKW
                     RHAPPVADRLQAMAWWDWDHDTLRKALPDFRALSAEAFLEKHERATSAPAVTGPLQRV
                     G"
     gene            2028..2489
                     /gene="phnG"
                     /locus_tag="AC16A0_07350"
     CDS             2028..2489
                     /gene="phnG"
                     /locus_tag="AC16A0_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HIR3105186.1"
                     /translation="MPQPEGSLDLLSRPQWMRLLALAQWTDLDARSRDLAGHPHDVLR
                     APETGLVMLRGRMGATGAAFNLGEATITRCAVRLTTGEEGHAYVLGRNADHAHRAALC
                     DALMQRPEDAGSIRQDVLSPIADAIRARQQRAAGKAAATKVDFFTLVRGDV"
     gene            2482..3075
                     /gene="phnH"
                     /locus_tag="AC16A0_07355"
     CDS             2482..3075
                     /gene="phnH"
                     /locus_tag="AC16A0_07355"
                     /inference="COORDINATES: protein motif:HMM:NF017645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HIR3105187.1"
                     /translation="MSDHVLSTTAVDSSYVFRTIMTAMSRPGRILAFLPPVMAPAPLL
                     PGTAAMLLTLCDFQTPIHLIPELADSAVTKYLRFHTGAPLTPDAADSSFAVLAAGLDM
                     PRLADFPQGTHEYPDRSATLLIQVPQVSEGGHVALRGPGIESTIEVTVTGMDAGFWHE
                     LMRNRDGFPIGVDVVFISPDAICAFPRSTTVSLRETA"
     gene            3075..4160
                     /locus_tag="AC16A0_07360"
     CDS             3075..4160
                     /locus_tag="AC16A0_07360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit"
                     /protein_id="HIR3105188.1"
                     /translation="MYVAVKGGEQAIDAAHCLLAEERRGDPTVPALTLAQITEQLTLA
                     VDRVMNEGSLYDRELAALAIKQARGDLIEAIFLIRATRTTLARFGYAVPLETRDMRVK
                     RRVSAAFKDIPGGQILGPTFDYTHRLLDAALVDGSEPEAARTAPASTGTAMPRVLDLL
                     GQEGLIEKPSGDGGEPGDLTQDPLNFPAGRDLRLQNLARGDEGFLLALAYSTQRGYGR
                     THPFAGEIRLGAVEVCFVPEELGFPVVIGEITVTECEMINQFKGSADVPPQFTRGYGL
                     GFGQCERKTMSMALVDRSLRAGELGEERFAPAQDEEFVLSHSDNVQATGFVEHLKLPH
                     YVDFQSELVLLRRMRADASRDRLEAAE"
     gene            4157..4993
                     /locus_tag="AC16A0_07365"
     CDS             4157..4993
                     /locus_tag="AC16A0_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HIR3105189.1"
                     /translation="MNAPAYNFAYLNEQTKRMIRRAILKAIAIPGYQVPFASREMPMP
                     YGWGTGGVQVTAAVLTPDDTLKVIDQGADDTTNAVSIRKFFIKTAAVKTTERTEEATI
                     IQTRHRIPETRLKEDQILVFQVPIPEPLRFLEPRETETRKMHALEEYGVMHVKLYEDI
                     ARYGQIATTYAYPAKVADRYVFDPSPIPKFDNQKLDNCPALQLFGAGREKRIYAIPPY
                     TSVKSLDFEDHPFEVQTWDQPCALCGSRDSFLDEIVTDDKGGRMFVCSDTDFCARQRG
                     EA"
     gene            4990..5760
                     /gene="phnK"
                     /locus_tag="AC16A0_07370"
     CDS             4990..5760
                     /gene="phnK"
                     /locus_tag="AC16A0_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HIR3105190.1"
                     /translation="MTPLLAAEGVTKCYGGRIGCSDVSMSLHAGEVLAIVGESGSGKT
                     TLLRCLSGHLPVTAGTVRYTLRDGRHVALNDLSEAERRLLMRTDWGFVHQDARDGLRM
                     GVSAGANVGERMMAVGDRHYGRIRATAVDWLTRVEIPEDRIDDSPWSYSGGMRQRLQI
                     ARNLVTSPRLVFMDEPTGGLDVSVQARLLDLIRGLVRKLDLAAIVVTHDLAVARLLST
                     RMMVMQQGRVIETGLTDQVLEDPQAPYTQLLVSSVLQV"
     gene            5763..6443
                     /gene="phnL"
                     /locus_tag="AC16A0_07375"
     CDS             5763..6443
                     /gene="phnL"
                     /locus_tag="AC16A0_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009032227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HIR3105191.1"
                     /translation="MAPVVRITNLDKTFHLHQQGSIRIPVLAGFSAEIDAGEAVAVTG
                     PSGRGKSSLLKLIYGSYRATGGAVRVWHEEDWVDVATAAPREVLAVRRRTIGYVTQFL
                     RVIPRVPAEVIVAEPLLDRGIPQETALERARHLLARLGIPQRLWHLSPMTFSGGEQQR
                     VNIARGFAAAFPVLLLDEPTASLDPANRSRVLDLIEEARAAGSAIIAVFHDDAERRRA
                     CNREIALG"
BASE COUNT         1200 a         2197 c         2065 g         1256 t
ORIGIN      
        1 cctagtcttt ccggcggtgc aacgcgtcca aggtgcgccc gcgaatggac cccagagacg
       61 ttctctgacg cgctcggcac cgctgcatat gccttggcat cacggcagga ggcggcggaa
      121 actgtcacat ggctgacatg agatgaggac atctaggcgc cttggatcta cagtgggagt
      181 cgccgtggcc tactttctgc tgctgttctc ggcagtcatg cttgccgttc acctggtcac
      241 tcagatcatc gttctcaggc gaaaatcatc acagatttcg tctgatgatc cggcaaccgc
      301 cgaggccgtg accatcgtcc ggccgatctc gcagatcgaa cctcacatct ggcagaccct
      361 ggaaagcaca ttccagatcg actggcccaa cctcgccatc gtcttctgcg ctgcacgcga
      421 gagcgatccg gctgtggcca tcgtacgcgc actgctcgac cgccacccgg acgcccatgc
      481 gcagttgctg cttggcgagt cccggatcag cgacaatccg aaactcaaca actgcgtgaa
      541 gggctggacc gccgcgcgga cggatgtcgt ggccttcgtc gattcgaatg tcctgctgcc
      601 gcgcgactat ctcaaggtca tgatggcatg ctggaacgag gagacgggga tgatcagcgt
      661 gcccgccgtc ggtgcagcgc ccggttcgtt ctgggccgag actgaatgtg ccatcctcaa
      721 tacccaccag gcgcgctggc aactcgttgc agatacgtgt ggcatgggtt tcgcccaggg
      781 caagaatctc atgttccgca agtcggtgct ggaccctctc ggcggaatcg ccgtgctcgc
      841 ccgcgaaccg gcggaagacg ccgccgccac caaagccatc cggcaggcga gcctctgcgt
      901 ccgccttgca ccgatgcctt ttccgcagcc gcttggcgcc aaaacattcc ggcaggtctg
      961 gaaccggcat ctgcgctggg caaagctgcg gcgcgccacc tttccgttgc agtacctgcc
     1021 cgaatttctg acagggagcg tcctgccgtt cacggccctg gtcagcgcgc tgttgattct
     1081 gggaattgat ctgctcgttc ccgcccttgt cctcgcggca gtctggttcg ggtccgaact
     1141 cgtcaccgcc agaaggctcg gctggcatgt ctcgccatgg acgccggtga gcttggccgc
     1201 ccgtgatctt ctgctgcccg ttctgtggat tgccggattt tcgccgggag gtttcgaatg
     1261 gcagggccat gccatgtcgg cccagcggtc tcacggcggc gctgcagagg cggaacacga
     1321 caccgacact gcaacgacgg tcacaaccta ggagccgcat atgcctgctc tcgcggagac
     1381 cccttttgtt cacgaaggag cggtggtccg cacgtcgcaa ctggggcgat acgtcgagat
     1441 tggcgcagga tcgcgcatcg tcgagtcgca gatcggcgac tacagctaca ccgacagata
     1501 tgcggacata gcctattcca cgctcggaaa gttcgcgaac gtggccgcat tctcgcggat
     1561 caatcctggc gaacatccct atcaccgggc atcgcttcac cacttcatgt atcgatccag
     1621 ctactactgg ccggacgaag ccgatgaagc cgcggtcttc gactggcgcc gctcgcggcc
     1681 cgttcgcatc ggccatgaca cctggatcgg ccatggcgcc atcatcatga agggcgtgac
     1741 cgttggaacc ggcgccatcg tagcctcgaa tgcggtcgtc accaagcccg ttccgcctta
     1801 tgcagtggtg gctggaacgc cggcgaagct gatcaagtgg cggcatgcgc caccggttgc
     1861 cgatcgactc caggccatgg cctggtggga ctgggaccac gacaccttgc gcaaggcatt
     1921 gccggacttc cgtgcacttt ccgccgaggc tttcctggag aagcatgaaa gggccacctc
     1981 tgcgccggct gtcacagggc cgttacaaag agttggttga tggggtcatg ccccagcctg
     2041 aaggttctct cgatcttctc tcgcgtccgc aatggatgcg gctgcttgcc ctcgcgcagt
     2101 ggactgacct cgatgcgcgg agccgcgatc tcgccggtca tccgcacgac gtccttcgcg
     2161 cgccggaaac cgggcttgtg atgctgcgcg gacgcatggg cgccaccggt gcggccttca
     2221 atctggggga ggcgactatc acgcgctgtg cggtccgcct gacgacgggt gaggaaggtc
     2281 acgcctatgt gctcggccgc aatgcagatc acgcccacag ggccgcgctc tgcgatgccc
     2341 tgatgcagcg gccggaagac gccggctcga tccggcagga cgtgctgtcg cccatcgccg
     2401 atgccatccg ggcaaggcag caacgcgcgg ccggaaaggc tgctgcaacg aaggtggatt
     2461 tcttcacgct ggtgcgcggc gatgtctgat catgtcctga gcacaactgc agtcgattcc
     2521 tcgtacgtct tccgcaccat catgacggcg atgtccagac ccggtcgaat cctcgcattc
     2581 ctgccgcccg tcatggctcc tgcaccgctc cttcccggaa cggccgccat gctgctcacc
     2641 ctctgcgatt tccagacgcc aatccatctc attcccgagt tggcagacag tgccgtgacc
     2701 aagtatctgc gcttccacac cggagcgccg cttacgcccg acgcggccga cagtagcttc
     2761 gccgttctgg cagccggact cgatatgcct cgcctcgccg actttccaca gggtacgcac
     2821 gaatatcccg accgctccgc aacgctgctc atccaggtgc ctcaggtctc tgaaggcggg
     2881 catgttgcac tgcgaggccc gggcatcgaa tcgaccatcg aagtgaccgt cacgggaatg
     2941 gatgccggct tctggcacga gttgatgaga aatcgcgatg gtttcccgat cggagtcgat
     3001 gtcgtgttca tctcgcccga cgcaatctgc gccttcccac gttcaaccac tgtcagcctg
     3061 agggagactg cctgatgtat gtggctgtca agggcggtga gcaggcgatc gatgcagccc
     3121 attgccttct tgcagaggaa cggcgcggcg atcccaccgt gcccgcactt acccttgccc
     3181 agatcacgga acagctgacg ctggcagtcg accgcgtgat gaacgaggga tcgctctacg
     3241 acagggagct cgccgcactg gccatcaagc aggcccgggg cgacctcatc gaggcgatct
     3301 tcctcatccg cgccacacgg acgactctgg cccgcttcgg ttacgcagtt ccgttggaaa
     3361 ccagggacat gcgggtgaag aggcgcgtgt cggcagcctt caaggacatt ccaggcggcc
     3421 agatccttgg acccactttc gactacaccc atcgtctgct cgatgccgcg ctcgtcgacg
     3481 gcagcgagcc ggaagctgca cggacggctc ccgccagcac cggcaccgcc atgccgcgtg
     3541 tcctcgatct cctcggccaa gagggattga tcgagaaacc ctcgggagac ggtggagaac
     3601 ccggcgatct cacgcaggat ccgctgaact ttccggccgg ccgcgatctc cggctgcaga
     3661 atctcgcgcg cggcgacgaa ggtttccttc tggccttggc ctattcaacg cagcgcggct
     3721 acgggcgtac acatcccttt gccggcgaaa tccggctggg cgcggtcgag gtctgctttg
     3781 tgcccgagga gctgggcttc cctgtggtga tcggcgagat caccgtgacc gaatgcgaga
     3841 tgatcaacca gttcaagggt tcggcggatg tcccgccgca gttcacccgc ggctacgggc
     3901 tgggcttcgg ccagtgcgag cgcaagacaa tgtccatggc cctcgtcgac cgttcgctgc
     3961 gggcgggcga acttggcgaa gagcgcttcg ccccggcaca ggacgaggaa ttcgtgctct
     4021 cgcattccga caatgttcag gcgaccggct tcgtcgagca tctcaagctg ccgcactacg
     4081 tggacttcca gtccgagctc gtgctgctca ggcgaatgcg ggccgacgcc agcagggacc
     4141 ggcttgaggc ggcggaatga acgcgccagc ctacaacttc gcctatctga acgagcagac
     4201 caagcggatg atccgtcgcg ccatcctgaa ggcgattgcc attcccggct atcaggttcc
     4261 cttcgcgagc cgcgagatgc cgatgcccta tggatgggga accggcggcg tgcaggtgac
     4321 cgcagccgtg ctgacaccgg atgacacgct gaaggtcatc gaccagggcg cggacgacac
     4381 caccaacgcc gtctcgatca ggaagttctt catcaagact gcggccgtca agacgaccga
     4441 acgcacggag gaggcgacga tcatccaaac gcgtcatcgc attcccgaga cacggctgaa
     4501 ggaagaccag atcctggtct ttcaggttcc gatccccgaa ccgctccgct ttctggagcc
     4561 gcgcgagacc gaaacccgca agatgcatgc gctggaggag tatggcgtca tgcatgtgaa
     4621 actctacgaa gacatcgcgc gttacggcca gatcgccacc acctatgcct atccggccaa
     4681 ggtggccgac cgctatgtct tcgatccctc gcccattccg aagttcgaca accagaagct
     4741 cgacaactgt ccggcactgc agcttttcgg tgcggggcgc gagaagcgca tctatgcgat
     4801 cccgccttac acgagtgtga agagcctgga tttcgaagac catcccttcg aagtccagac
     4861 ctgggaccag ccttgcgcgc tctgcggatc gcgcgacagc ttcctcgacg agatcgtgac
     4921 agacgacaag ggcggacgga tgttcgtctg ttcggacacc gacttttgcg ccaggcagcg
     4981 aggcgaggca tgacgccact tctcgcggcc gagggagtca cgaagtgcta cggcgggcgc
     5041 atcggctgca gcgatgtcag catgtcgctc cacgccggcg aagtacttgc gatcgtgggg
     5101 gagtccggat ccggcaaaac cacgctcctg cgctgcctct ccggccattt gccagtcacc
     5161 gccgggacag tccgctacac cctgcgcgac ggccgccatg tggcgctgaa cgacctcagc
     5221 gaagcagagc gtcgtctcct gatgcggact gactggggct tcgtccatca ggacgcgcgc
     5281 gacggcctgc gcatgggtgt ctccgccgga gccaatgtcg gcgaacgcat gatggcggtt
     5341 ggcgaccggc attatggccg catccgtgcg accgccgtcg actggttgac gcgggtcgag
     5401 attcccgaag accggatcga cgacagcccg tggtcctatt ccggcggcat gcggcagcgc
     5461 cttcagatcg cgcgaaacct cgtgacctcg ccccgcctgg tcttcatgga cgagcccaca
     5521 ggcggcctcg atgtctcggt gcaggcaagg ctgcttgacc tcattcgcgg acttgtccgc
     5581 aagctcgacc tggcagcgat tgtcgtgacc catgacctcg ccgtcgcgcg gctgctgtcg
     5641 acccggatga tggtgatgca gcagggacgg gtcatcgaga cagggctgac ggaccaggtt
     5701 ctcgaggatc cccaggcacc ctacacccaa ctccttgtct cctccgtgtt gcaggtgtga
     5761 cgatggctcc ggtcgtgagg atcacgaacc tcgacaagac atttcatctt caccagcagg
     5821 gatcgatcag gattcccgtg ctcgctggct tctccgcgga gatcgacgcc ggagaggccg
     5881 tggcggtcac gggcccctcg ggtcgtggca agagctcgct tctgaagctg atctacggca
     5941 gctaccgcgc gaccggcggc gcagtccgcg tctggcatga ggaggattgg gtcgatgtgg
     6001 cgaccgcagc accgcgggaa gtgcttgccg tgcggcggcg tacaatcggt tatgtcaccc
     6061 agttcctgcg cgtcatcccg cgcgtgccgg ccgaagtgat cgtggccgaa ccgctgctgg
     6121 atcgcggtat tccgcaggag acggcgcttg aaagggcaag acatcttctc gcccggctcg
     6181 gcattcccca acgcctctgg catctctcgc ccatgacctt ctcgggcggc gagcagcagc
     6241 gcgtcaacat cgcgcggggc tttgcggcag ctttccccgt gttgcttctc gacgaaccca
     6301 cggcctcgct cgacccggca aacaggtcgc gcgtgctgga tctgatcgag gaagcccgtg
     6361 ctgctgggag cgcaatcatc gctgtctttc atgacgacgc cgagcgccgc cgcgcttgca
     6421 accgggagat tgcgcttggc tagcgacgaa acatcagcac tgcggtttgc gatctacttc
     6481 gcaccaccac cggggagcct gctgcacgaa cgcggcgcgt cgtggcttgg gcgcgatgcc
     6541 catggggcgt ctgtgagtcc agtccggaac tccgacttgt ccgatgacct cattgcggat
     6601 gcgcggcgct acggtttcca cgcgacgctg aagccgccct tcatcctgag aaatggttgc
     6661 acggaagcag ccttgactgt tgccattgcg catctcgcat ctggtctcga cgccgtga
//
LOCUS       DBNGVR010000349        10314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3468845, whole genome shotgun sequence.
ACCESSION   DBNGVR010000349 DBNGVR010000000
VERSION     DBNGVR010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3468845"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC16A0_07380"
     CDS             <1..321
                     /locus_tag="AC16A0_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR3105192.1"
                     /translation="IARDILSQDALKPFLMAERLPGPERTSDEDVFDYVCGMAKTQHH
                     PACSCAMGQGKMAVVSPQLKIHGLDGLRVADSSVMPFVVSSNTNAATVMIAEKCADMI
                     KGGS"
     gene            321..1340
                     /locus_tag="AC16A0_07385"
     CDS             321..1340
                     /locus_tag="AC16A0_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3105193.1"
                     /translation="MNRIERRKFGRIDLTVTDMGFGAAPIGNFLRPIDEATSDAMVQH
                     AWNAGMRYFDTAPYYGHGLSELRLAHSLRWKPRGEYVISSKVGRVLKPERRSRIDFKP
                     WVDALPNVCRFDYSYDGTMRSFEDSLQRLGLEHIDILFIHDADVFTHGAEQQKIHCRT
                     AMEGCYPALRKLKESGAVKAIGVGVNNWEIMLDFMKAGDFDCLLMAGRYTLLEQDPLD
                     ELLPLCEKRGAAIVIGGGLNSGILATGAVPGAKYNYATAPEHIMEKVRRIEAVCAAFK
                     VPLPAAALQFLLAHPAVASHVPGTRTVEQMQQNIDWISHPIPKDFWAELKHEGLLRED
                     APVPA"
     gene            complement(1417..1638)
                     /locus_tag="AC16A0_07390"
     CDS             complement(1417..1638)
                     /locus_tag="AC16A0_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105194.1"
                     /translation="MMGKKKVKAIEGFVMFDVVYEDGTRSSRRKVAAAEVAEFGETHA
                     KTVIMDQDRKIAEVSGHSRGAIQSISRSG"
     gene            complement(1635..1808)
                     /locus_tag="AC16A0_07395"
     CDS             complement(1635..1808)
                     /locus_tag="AC16A0_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105195.1"
                     /translation="MAFPPNYNQERNNRARAKAQKALDKQNEREDKSARRKRVRAAED
                     PAPAPEPAKTEKR"
     gene            complement(1840..2073)
                     /locus_tag="AC16A0_07400"
     CDS             complement(1840..2073)
                     /locus_tag="AC16A0_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105196.1"
                     /translation="MSIAEANLYMFKSSKSQGLCSFSRNPHGKDLPEKFAPWTAFGVV
                     RSDQRPPHGLSREAIETGIDANGYQLWRDKRKV"
     gene            complement(2207..2416)
                     /locus_tag="AC16A0_07405"
     CDS             complement(2207..2416)
                     /locus_tag="AC16A0_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3105197.1"
                     /translation="MQTGIVKWFNAQKGFGFIQPTSGGPDVFVHVSAVERAGMSGLNE
                     GQKLSYEIVADKRSGKSSADNLQTA"
     gene            2714..4129
                     /locus_tag="AC16A0_07410"
     CDS             2714..4129
                     /locus_tag="AC16A0_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3105198.1"
                     /translation="MKTTGSGVRYIETGAGEAVLLVHGRGGDGQQWSAEIEGLAVRHR
                     VIVAVLAGQTPAESSGALRRLLDDLAVPAVTVVSRADDAGAALAFTLAHPIRVRRLAL
                     IAPPLESLVAASLEDIRCPVMMTGDASSLERHLARVSRGRLHPTPRTPAETGQLIEEF
                     LAGRLSSFDPRELRTAFGTFMTGVTIVTTSGADGQPRGFTANSFTSLSIEPPLLLICI
                     GKGAASREVFESARGFAVNILSEHQKDTSVLFASKRPDKFQHTTWRPGPFGNPLIDSA
                     AAWFDCARHQVIDAGDHIILIGHIEAFSYTDANPLGYARGGYITLGLEQAAVNAASSA
                     SRTIVGAILESDNRLVLLPDHRPNGLSLPEVGRSGSSGSASRLHALLAQDGIDATLGF
                     LFAVFENPGSGEQCIYYRGEALLHARGRPVLASFDDIPWDRLPDEATRSMLRRYAAER
                     KLGRFKVYSGDHRQGTVKAVE"
     gene            4238..5263
                     /locus_tag="AC16A0_07415"
     CDS             4238..5263
                     /locus_tag="AC16A0_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3105199.1"
                     /translation="MARATIEDVADAAGVSVATVDRVLNGRAAVRPKTVERVEKAIRL
                     LNYQSDRLAARLARAKEYRFCFILPEGSNAYMTLLADAVREEALRMAQEKVVIDLRHT
                     DVFSAEVLGQTLDQISGLYDGVAAVALDHPRVRESINALCERGVAVVTLVSDVPGSRR
                     THYVGIDNAAAGRTAATLMGRYLAGRRGKVGLIAGSLSLRDHVERQFGFEQVMLHEYS
                     GLTLLPVREGRDDWQIVQGVAAQLLHDHPDLIGLYNVGAGTRGIVAALEAAGRQRKVV
                     YIAHELTDESRRALIDGTIDIVINQDAGHEVRSAIRVLMSRADRSPIVEAMERIRIDI
                     YVRDNIP"
     gene            5273..6730
                     /gene="xylB"
                     /locus_tag="AC16A0_07420"
     CDS             5273..6730
                     /gene="xylB"
                     /locus_tag="AC16A0_07420"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3105200.1"
                     /translation="MYLGLDFGTSSVKGVLIDATQKIIATASAPLKVSRPHPGWSEQN
                     PEDWWKACNIVVKTLGRMKPKAVAAVEGIGLSGQQHGATMLDAGGKALRPCILWNDAR
                     SFRECEEIMAREPKAVPLAGNVPLAGYTAPKLVWVKKHEPRIFDKVAKVLLPKDYIRF
                     RMTGDYASDMSDSSGTFWLDIAKREWSDLLLAASDMRRDQMPKLYEGTDATGRLTPAV
                     AKAWNMPKRPVVAGGGGDNAAAACGIGAVTDNSALVSIGTSGVLFVSNDRFRPNAGRM
                     VHAFCHAVPDTWHQMGVILSAAASLDWLAGILGSPAPKLIGGLGKTLAGPSPALFLPY
                     LSGERTPVGDAQVRGLIMGLGHETDHRMLTHAALDAVAFAFRDSLEALKDAGTKVKRV
                     TAVGGGSRSDLWLQIIATVLGVPVDLPAAGDVGGAFGAARLGLIAATGADFRKVLTAP
                     KTARTIKPNLKAREAYEAQYRRYTQIYPAIKEIYR"
     gene            6730..8043
                     /gene="xylA"
                     /locus_tag="AC16A0_07425"
     CDS             6730..8043
                     /gene="xylA"
                     /locus_tag="AC16A0_07425"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HIR3105201.1"
                     /translation="MTTFYSRKDPIRYEGPSSTSELAFRWYNPDQKVLGKTMREQMRF
                     AVAYWHTLCWPGTDPFGGDTFQRQWHHMGDEMAAARMKADIMFETLELLDVDFFCFHD
                     LDIAPEGRSLKEFNANVTAIAKNFEKKMAAKNKKLLWGTANMFSNRRFMAGAATNPDP
                     DVFAYCAAQVKHCLDVTKDLDGANYVMWGGRECYETLLNTNIGHELEQAKRFLSMVVE
                     YKHKIGFKGPILLEPKPQEPTKHQYDYDVATCYATIASLGLQKDVKLNIEQNHAILAG
                     HTFEHEIALASALGVFGGIDINRGDYQSGWDTDQFAMNIPEMALAFLEILKAGGFTTG
                     GLNFDAKIRRQSIDADDILHAHVGSMDACARGLLMAAKIIEDGKLKGAVDQRYGKWKE
                     PQNLAMLSGKETLDSIAARVLSSGLDPQPASGRQERLENLVNSYI"
     gene            8065..9123
                     /locus_tag="AC16A0_07430"
     CDS             8065..9123
                     /locus_tag="AC16A0_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3105202.1"
                     /translation="MATNIKGPAIFLAQFAGDAAPFNSWDSITKWAASLGYKGVQIPS
                     WDGRMIDLKKAAESKTYCDEIKGVAKQNGVEITELSTHLQGQLVAVHPAYDEAFDAFA
                     PASFHGKPKERQAWAVQQVKYAAKASRNLGLGAHVSFPGALCWPFMYGWPPRPAGLIE
                     EGFDELARRWKPLLDVFDENGVNVGFELHPGEDVMDGATFEMFLERLNNHPRCMINYD
                     PSHFVLQQLDYLAFIDIYKDRICAFHVKDAEFNPTGKQGVYSGFQPWINRAGRFRSLG
                     DGQVDFAAIFSKMAANNYDSWAVLEWECCLKDSVQGAMEGAPFIESHIIKVTEKSFDD
                     FVAGSADKAKIHRILGIK"
     gene            9135..10304
                     /locus_tag="AC16A0_07435"
     CDS             9135..10304
                     /locus_tag="AC16A0_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3105203.1"
                     /translation="MVSGTSEKNTGRIRLGMVGGGQGAFIGAVHRLAARMDDHYDFVA
                     GALSAEAKKAKASGEELGLAAERIYTDFKEMAKSEAGRKDGIEAVAIVTPNHMHVPAA
                     MAFIEHGIHVICDKPLALSLKEAKALEKLLARKKNVIFALTHNYSGYPMIRQAKAMVA
                     AGELGEIRVVQGEYPQDWLTTALEKTGQKQAAWRTDPKRSGAGGCIGDIGTHTYQLMC
                     EVSGLKAEELSADLTSFVKGRVLDDNANVMLRFKGGAKGMLWASQVAPGNENGLKIRV
                     YGTKGGLEWKQEDPNQLWYTPYGEPKRLITRGGAGSNAAAGRVTRVPPGHPEGYLEGF
                     ANVYSEVARAIKARRLGRKPDKDVAFPGISDGVDGMAFIEACVKSSAKNGKWTKL"
BASE COUNT         1902 a         3434 c         3214 g         1764 t
ORIGIN      
        1 atcgcgcgcg acatcctcag ccaggatgca ctcaagccat tcctgatggc cgagcgtctg
       61 ccgggtccgg agcgcacctc tgatgaggat gtattcgact atgtctgcgg catggccaag
      121 acgcagcacc acccggcctg ctcctgcgcc atgggccagg gcaagatggc ggtcgtctct
      181 ccgcagctga agattcacgg cctcgacggc ctgcgtgtgg cggactcctc cgtcatgccc
      241 ttcgtcgtgt cgtccaacac caatgctgcg accgtcatga tcgccgagaa atgcgccgac
      301 atgatcaagg gaggatcgtg atgaaccgca tcgaaaggcg aaagttcggc cggatcgacc
      361 tgaccgtcac cgatatggga ttcggcgccg ctcccatcgg caatttcctg cgccccatcg
      421 acgaggcgac atcggacgcc atggtgcagc atgcctggaa cgccggcatg cgctacttcg
      481 atacggcacc ctactacggc cacggccttt cggaactgcg gcttgcccac agcctgcgct
      541 ggaagccgcg gggcgaatac gtgatttcga gcaaggtcgg gcgcgtcctc aagccggagc
      601 ggcggagcag gatcgacttc aagccctggg ttgatgccct gcccaatgtc tgccgcttcg
      661 attacagcta tgacggcacg atgcggtctt tcgaagactc gctgcagcgc ctcggtctcg
      721 agcacatcga catcctcttc atccatgatg cggatgtctt cacgcatggc gccgaacagc
      781 agaagatcca ttgcaggact gcgatggaag gctgctatcc agccttgcgc aagctaaagg
      841 agagcggcgc cgtcaaggcc atcggggtgg gcgtcaacaa ctgggagatc atgctggact
      901 tcatgaaggc gggcgatttc gactgcctgc tcatggccgg gcgctacacg ctgctcgaac
      961 aggaccctct cgacgagctc ctgccgctct gcgagaaacg cggcgcggcg atcgtgatcg
     1021 gcggcggcct caacagtggc atccttgcca ccggtgcggt acccggcgcc aagtacaact
     1081 acgcgacggc gcccgaacac atcatggaga aggtccgcag gatcgaagct gtctgtgccg
     1141 ccttcaaggt gccgctcccg gcggcggccc tgcagttcct tctggcgcac cctgccgttg
     1201 ccagccatgt gccgggaacc cgcaccgtcg agcagatgca gcagaacatc gactggatct
     1261 cccatcccat cccgaaggac ttctgggcgg aactcaagca cgaggggctt cttcgcgagg
     1321 acgcgcccgt cccggcttga tatggtcttg gccacacgtc acgctccggg cggccaaggc
     1381 cgtcgcgcat tttggtgtcg ggagaacagt aatccctcaa cccgagcggc tgatcgactg
     1441 gatggcaccc cggctatgcc cggaaacctc cgcgatcttg cggtcctgat ccatgatcac
     1501 ggtcttcgca tgggtctcgc cgaattcggc cacctcggcc gcggccactt tgcggcggga
     1561 actgcgggtg ccatcctcat agacgacgtc gaacatcaca aagccttcga tggccttgac
     1621 cttcttcttg cccatcatcg tttctccgtc ttggccggct ccggggcggg agcgggatcc
     1681 tcggctgcgc gaactctctt gcggcgggcc gacttgtcct cgcgctcgtt ctgcttgtcg
     1741 agcgccttct gcgccttggc acgggcgcgg ttgttgcgtt cctgattgta gtttggcgga
     1801 aatgccatcg catcgtctcc ggatcggttg gcgggtgggc tagaccttgc gcttgtcgcg
     1861 ccagagctga tagccattgg catcgatgcc ggtctcgatc gcttcgcgcg acagcccgtg
     1921 cggcggccgc tggtcgctgc ggaccacccc gaaggcggtc cagggcgcga acttctccgg
     1981 cagatccttg ccatgcggat tgcgcgagaa gctgcacagc ccctgcgact tcgacgactt
     2041 gaacatgtag agattggcct cggcgatgga catgatgtca ctccggttgg atggcaggtc
     2101 gaactgaaag ccgctctgcg ccggttcaat gaccggcgca tcgcgtcgcc ccgtcggccc
     2161 gccaccagta catccgtggc tcgcgggcgt tcgaaagcgt gaaacctcag gcggtctgaa
     2221 gattgtcggc cgaggacttg cccgaccgct tgtccgccac gatctcgtag gagagcttct
     2281 ggccttcgtt cagaccgctc attccggcgc gttcaacggc gctgacatga acgaaaacat
     2341 cgggaccgcc cgatgtcggc tgaatgaacc cgaagccctt ctgggcattg aaccacttca
     2401 caattcctgt ttgcacagcc atctcctgag taatgcaaag gcacaaaccc gcagacgctc
     2461 accatgagcg cttcgggaaa tcgatgttct ggatgtaaaa gtagggctgt tgcacttgca
     2521 gtgagaagcg cggcgccgag ccaaacggcc gaaactcgat ggaacctata tgggtcagtt
     2581 cgtcctgcaa tacaagcgat tccgccgtcc gcggccaaga agaaacgcca cgacgcgcag
     2641 cgccggttga cccttcacgg aggttccggc aaatggcggg atctggaagc tggatcctgt
     2701 catggtgcat tcattgaaaa cgaccggaag tggcgtgcgc tacatcgaga cgggtgctgg
     2761 cgaggccgtt ctgctggttc atggccgcgg cggggatggg cagcagtggt cggccgagat
     2821 cgaaggtctc gccgtccggc accgcgtgat tgtggcggtc ttagctggcc agacgcctgc
     2881 ggaatcatcc ggcgccctcc gccgcctgct ggacgacctg gcggtgcctg cagtcaccgt
     2941 cgtttcacgc gcggacgatg ccggggcggc gctggctttc acgctggcgc atcccatccg
     3001 cgtacgccgc cttgcgctga tcgcgccgcc tctggagagt cttgtggcgg caagccttga
     3061 agacatccgc tgtcccgtga tgatgaccgg cgacgcctcc agcctcgagc gccatctcgc
     3121 acgtgttagc cgcgggcgtc ttcacccgac gccccggacc ccggcggaga ccggccagtt
     3181 gatcgaggag ttcctcgccg gccggctgag cagcttcgat ccgcgcgaac tgcggaccgc
     3241 cttcggcacc ttcatgaccg gcgtgacgat tgtcaccaca tccggagccg acgggcagcc
     3301 acgcggcttc accgccaaca gcttcacgtc gctctccatc gaaccgccgc tgctgctcat
     3361 ctgcatcggc aagggtgccg cgagccggga ggttttcgaa tccgcccgcg gcttcgcggt
     3421 gaacatcctg tccgaacacc agaaggacac ctccgtgctc ttcgcctcca agcggccgga
     3481 caagttccag cacaccacct ggcgtcccgg accattcggc aatccgctca tcgacagcgc
     3541 ggcggcctgg ttcgactgcg cccgccacca ggtgatcgat gccggcgacc acatcatcct
     3601 catcggtcac atcgaggcct tctcctatac cgacgccaat ccgctgggct atgcgcgcgg
     3661 cggctacatc acgctgggcc tcgaacaggc cgcggtgaat gcggcgtcgt ccgccagccg
     3721 caccatcgtg ggtgccatcc tcgagagcga caaccgtctc gtgctgctgc ccgatcaccg
     3781 gcccaacggc ctgtcgcttc cggaagtcgg ccggagcggt tcgagcggca gcgcctcccg
     3841 gctgcacgcg ctgctggccc aggacggcat cgacgccacg ctcggatttc tcttcgccgt
     3901 cttcgaaaat cccggctccg gcgagcagtg catctactac cgcggcgaag ccctgctgca
     3961 tgcccgcggc cggcccgtgc tggcgagttt cgatgacatt ccctgggatc gcctgccaga
     4021 cgaggccacg cggtccatgc tccggcgcta tgccgccgag cgcaagctcg ggcgcttcaa
     4081 ggtctattcc ggcgatcacc gccagggcac ggtgaaggcc gtcgagtgat ccgcgtcctg
     4141 ctgcagtgca acgccgcccg ctccatcgca tttgaaagct tctgatagac gcctatcagg
     4201 aattgacatc cagccggaaa ctgactgtga tgacgccatg gcaagggcaa ccatcgaaga
     4261 cgtggcggat gcggcgggcg tcagcgtggc aacggttgat cgcgtgctca atggccgcgc
     4321 cgctgtccgg cccaagacgg tcgaacgcgt cgagaaagcc atccggctcc tcaactatca
     4381 gtcggaccgg ctggccgcac gccttgcgcg cgcgaaggaa tatcgcttct gcttcatcct
     4441 gccggagggc tccaacgcct acatgacgct gctggccgac gcggtgcgcg aagaggccct
     4501 tcgcatggcg caggagaagg tggtgatcga tctgcgccac accgatgtct tctcggccga
     4561 ggtgctgggc cagacgctcg accagatcag cgggctctat gatggcgtcg ccgccgtggc
     4621 cctcgaccat ccacgcgtgc gcgagtcgat caatgcattg tgcgagcgcg gcgttgcggt
     4681 ggtgacgctg gtgtcggatg tgccgggttc gcgccgcacc cattatgtgg ggatcgacaa
     4741 tgccgccgcc ggccgcaccg ccgcgacgct gatgggccgc taccttgcgg gccggagggg
     4801 caaggtcggg ctgatcgcgg ggtcgctgtc gctgcgcgat catgtcgaac gccagttcgg
     4861 cttcgaacag gtcatgctcc atgagtattc cggcctcacc ctgctgccgg tgcgcgaagg
     4921 ccgcgacgac tggcagatcg tccagggcgt tgcagcccag ctgctgcacg accatccgga
     4981 cctcatcggc ctctacaacg tgggcgcggg aacgcgcggc atcgtggccg cgctggaagc
     5041 cgcagggcgc cagcggaaag tcgtctacat cgcgcatgaa ctcaccgacg aatcccgccg
     5101 cgcgctgatc gatggcacca tcgacatcgt gatcaaccag gatgccggcc atgaagtgcg
     5161 cagcgccatc cgggttctga tgtcgcgcgc cgaccgctcg cccatcgtcg aggcgatgga
     5221 acgcatccgc atcgacatct acgttcgcga caacattccc tgaggcaacc ccatgtatct
     5281 cggtctcgac ttcggcacct catcggtcaa gggcgttctc atcgacgcca cgcagaagat
     5341 catcgccacg gccagcgcgc cgctgaaggt ctcgcggccg catcccggct ggtcggagca
     5401 gaatccggaa gactggtgga aggcctgcaa cattgtggtc aagaccctgg gccggatgaa
     5461 gcccaaggcc gtggcagcgg tggaaggcat cggcctttcc ggccagcagc atggcgccac
     5521 gatgctcgac gcaggcggaa aggcactcag gccctgcatc ctgtggaacg atgcgcgctc
     5581 cttcagggag tgcgaggaga tcatggcgcg cgagcccaag gcggtgccgc tcgccggcaa
     5641 tgttccgctc gccggctaca ccgcgccgaa gctcgtgtgg gtgaagaagc acgagccgcg
     5701 catcttcgac aaggtggcaa aggtcctgct gcccaaggac tacatccgct tccgcatgac
     5761 cggcgactat gcctccgaca tgtcggattc ctcgggcacc ttctggctcg acatcgccaa
     5821 gcgcgaatgg tcggacctgc tgctcgccgc cagcgacatg cggcgcgacc agatgccgaa
     5881 gctctatgaa ggcaccgatg ccacgggccg cctcacgcca gccgtcgcca aggcctggaa
     5941 catgccgaag cggccggtgg tggcgggtgg tggcggcgac aatgcggcgg ccgcctgcgg
     6001 catcggcgcg gtcaccgaca actccgccct ggtctccatc ggaacgtcgg gggtgctctt
     6061 cgtctccaac gacaggttcc gccccaatgc cggccgcatg gtgcatgcct tctgccatgc
     6121 cgtgccggac acctggcacc agatgggcgt catcctctcc gccgcggcca gcctcgactg
     6181 gctggccggc atcctcggtt ccccggcgcc gaagctgatc ggcggcctcg gcaagacgct
     6241 cgccggcccg tcgcccgctc ttttcctgcc ctatctctcg ggcgaacgca cgccggtggg
     6301 cgacgcgcag gtgcgcggcc tgatcatggg gctgggccat gaaaccgacc accggatgct
     6361 gactcatgca gccctcgatg ccgtggcctt cgccttccgc gacagcctcg aagccctcaa
     6421 ggatgcgggc accaaggtga agcgcgtgac ggcggtgggc ggcggctcgc gctccgatct
     6481 ctggctccag atcatcgcca cggtcctcgg cgtgccggtc gacctgccgg cggccggcga
     6541 cgtcggcggc gccttcgggg ccgcgcgcct cgggctcatt gccgcaaccg gcgccgactt
     6601 ccgcaaggtg ctcaccgcac caaagacggc ccgaacaatc aagccgaacc tgaaggcgcg
     6661 cgaggcctac gaggcgcagt atcgccgcta cacgcagatc tatcccgcca tcaaggagat
     6721 ttaccgctga tgaccacatt ctattcccgg aaggacccga tccggtacga aggtccctcc
     6781 tccaccagcg aactggcgtt ccgctggtac aatcccgacc agaaggtgct cggcaagacc
     6841 atgcgcgagc agatgcgctt cgcggtggcc tactggcaca cgctgtgctg gccggggaca
     6901 gaccccttcg gcggcgatac gttccaacgc cagtggcatc acatgggcga cgagatggcg
     6961 gcggcccgca tgaaagccga catcatgttc gagacgctcg aactcctcga tgtcgacttc
     7021 ttctgcttcc acgatctcga catcgcgccg gagggccggt cgctcaagga gttcaacgcc
     7081 aacgtcacgg cgatcgccaa gaacttcgag aagaagatgg cagccaagaa caagaagctg
     7141 ctgtggggaa ccgccaacat gttctccaac cgccgcttca tggcgggcgc ggccaccaat
     7201 cccgatcccg atgtcttcgc ctattgcgcg gcacaggtga agcactgcct cgacgtgacc
     7261 aaggatctcg acggcgccaa ctacgtcatg tggggcggcc gcgagtgcta cgagacgctg
     7321 ctcaacacca acatcggcca tgagcttgaa caggccaagc gcttcctctc catggtcgtc
     7381 gaatacaagc acaagatcgg cttcaaggga ccgatcctgc tcgaacccaa gccgcaggaa
     7441 ccgaccaagc accagtacga ctatgatgtg gccacctgct acgcgacgat cgcatccctc
     7501 ggcctgcaga aggacgtgaa gctcaacatc gaacagaacc acgccattct cgccggccac
     7561 accttcgagc acgagatcgc gctggcctcc gccctcggcg tcttcggcgg catcgacatc
     7621 aaccgcggcg actaccagtc gggatgggac accgaccagt tcgccatgaa catcccggaa
     7681 atggccctgg ccttcctcga gatcctgaag gccggtggct tcaccaccgg cggtctcaac
     7741 ttcgacgcca agatccgccg ccagtcgatc gacgcggacg atatcctgca tgcccatgtc
     7801 ggctccatgg atgcctgcgc ccgcggcctg ctgatggcgg ccaagatcat cgaagacggc
     7861 aagctcaagg gtgccgtcga ccagcgctac ggcaaatgga aggagccgca gaacctggcc
     7921 atgctgtcgg gcaaggaaac gctcgactcc atcgcggcgc gcgtgctgtc ctccggactc
     7981 gatccgcagc cggcgtcagg acggcaggaa cgcctcgaaa atctcgtcaa ttcgtacatc
     8041 taaggtcagg gaggacccaa gcacatggca accaacatca agggaccggc gatcttcctc
     8101 gcgcaattcg cgggcgatgc ggcgccgttc aattcctggg actcgatcac caagtgggcg
     8161 gcttcgctcg gttacaaggg cgtgcagatc cccagctggg acggccgcat gatcgacctg
     8221 aagaaggccg cggagtcgaa gacctactgc gacgaaatca aaggcgtggc gaagcagaac
     8281 ggcgtcgaga tcaccgagct gtcgactcat ctgcagggcc agctggtggc cgtgcatccg
     8341 gcctatgacg aggccttcga tgccttcgcg cccgcaagct tccacggcaa gcccaaggag
     8401 cgtcaggcct gggccgtgca gcaggtcaag tatgcagcca aggcctcgcg caacctcggc
     8461 ctcggcgcgc atgtgtcctt ccccggtgcg ctgtgctggc ctttcatgta tggatggccg
     8521 ccgcgcccgg cgggcctgat cgaggagggc ttcgacgagc tggcgcgccg ctggaagccc
     8581 ctgctcgacg tcttcgacga gaatggtgtc aacgtcggtt tcgaattgca tcccggcgaa
     8641 gacgtcatgg acggcgccac cttcgagatg ttccttgagc gcctcaacaa ccacccgcgc
     8701 tgcatgatca actacgatcc atcgcacttc gtgctgcaac agctcgacta cctggccttc
     8761 atcgacatct acaaggaccg catctgcgcc ttccacgtga aggacgcgga gttcaatccc
     8821 actggcaagc agggcgtcta ttccggcttc cagccctgga tcaaccgtgc cggccgcttc
     8881 cgctcgctgg gcgacggaca ggtggatttc gcggcgatct tctcgaagat ggcggcgaac
     8941 aattacgact cgtgggccgt gctcgaatgg gaatgctgcc tcaaggattc ggtgcagggg
     9001 gccatggaag gcgcgccctt catcgagagc cacatcatca aggtcacgga aaaatccttc
     9061 gacgacttcg tggcgggctc agccgacaag gccaagatcc accgtatcct cggcatcaag
     9121 taaggacgaa ggccatggtt tccggaacaa gcgaaaagaa cacgggccgc atccgcctgg
     9181 gcatggtggg cggcggacag ggcgccttca tcggcgccgt tcaccggctt gccgcgcgca
     9241 tggacgatca ctacgacttc gtggcgggcg cgctgtcggc cgaggcgaag aaggccaagg
     9301 cctccggcga ggaactcggc ctcgctgccg agcgcatcta caccgacttc aaggagatgg
     9361 cgaagtccga agccgggcgc aaggacggca tcgaggcggt ggctatcgtc acgccgaacc
     9421 acatgcatgt tccggcggcg atggccttca tcgagcatgg catccatgtc atctgcgaca
     9481 agcccctggc gctgtcgttg aaggaggcca aggcgctcga gaagcttctg gcgcgcaaga
     9541 agaacgtgat cttcgcgctc acgcacaact actcgggcta cccgatgatc cgccaggcca
     9601 aggccatggt ggcggcgggc gagctgggcg agatccgcgt cgtccagggt gagtatccgc
     9661 aggactggct caccaccgca ttggagaaga cggggcagaa gcaggccgcc tggcgcaccg
     9721 acccgaagcg gtccggcgcc ggcggttgca tcggcgacat cggcacccac acctaccagc
     9781 tcatgtgcga agtctccggc ctcaaggccg aggaactctc ggctgatctc acgtccttcg
     9841 tgaagggccg ggtgctggac gacaacgcca atgtcatgct gcgcttcaag ggcggcgcca
     9901 agggcatgct ctgggccagc caggtcgcgc cgggcaacga gaacggtctg aagatccgcg
     9961 tctatggtac caagggcgga ctcgagtgga agcaggaaga tcccaaccag ctctggtaca
    10021 cgccctatgg cgagccgaag cgcctgatca cccgcggcgg tgcgggatcg aatgctgcag
    10081 ccggccgcgt cacgcgcgtc ccgccgggcc atccggaagg ctatctggaa ggcttcgcca
    10141 acgtctattc ggaagtggcc agggccatca aggcacgccg cctcggccgc aagcccgaca
    10201 aggacgtggc cttccccggt atctccgatg gcgtggacgg catggccttc atcgaagcct
    10261 gtgtgaagtc ctcggcgaaa aacggcaagt ggacaaagct ctgaccccgt ccag
//
LOCUS       DBNGVR010000350         3497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4844457, whole genome shotgun sequence.
ACCESSION   DBNGVR010000350 DBNGVR010000000
VERSION     DBNGVR010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4844457"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..203)
                     /gene="yacG"
                     /locus_tag="AC16A0_07440"
     CDS             complement(12..203)
                     /gene="yacG"
                     /locus_tag="AC16A0_07440"
                     /inference="COORDINATES: protein motif:HMM:NF002362.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR3105204.1"
                     /translation="MTDPVRLRPKRPCPICSKPSQQKFHPFCSNRCAQIDLSRWLGGH
                     YAIPAVEAEDDRTENSDES"
     gene            complement(200..841)
                     /locus_tag="AC16A0_07445"
     CDS             complement(200..841)
                     /locus_tag="AC16A0_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HIR3105205.1"
                     /translation="MAESPVKLVLASASPRRLQLLERIGLVPDLLNPADIDETPRKRE
                     TPRAHSIRLAGEKATVALDMPQVRALGPNIFLLAADTVVGLGRRIIPKAETEDEARDC
                     LSLLSGRSHWVYSTVVLVAPGRRTSRRCVETKVRFKRLSREDIDIYIASGEWRGKAGA
                     YAIQGRAEAFVRMLTGSHSAVVGLPLYETVHLLEGAGYPVFHSWMTGAGAEAP"
     gene            complement(848..1066)
                     /gene="infA"
                     /locus_tag="AC16A0_07450"
     CDS             complement(848..1066)
                     /gene="infA"
                     /locus_tag="AC16A0_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3105206.1"
                     /translation="MAKEELLEFEGTVTELLPNATFRVKLDNNHEIIAHTAGKMRKNR
                     IRVLAGDRVMVEMTPYDLTKGRINYRFK"
     gene            complement(1122..1619)
                     /locus_tag="AC16A0_07455"
     CDS             complement(1122..1619)
                     /locus_tag="AC16A0_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HIR3105207.1"
                     /translation="MRHFTFRHQPPSPLRTAILGGLGAMLAVAAIGLTQDLAGLTMLF
                     APLGATCVLVFGVPASPLSQPVNVVLGHALAGVIGVGAHLALPDSVWIAAVAVGLAIT
                     GMAIFRVTHPPAGATALVSYAGAQSAAFLAFPILSGAVALVLLGTAYHRLTGTTYPAK
                     PPERA"
     gene            complement(1659..2606)
                     /locus_tag="AC16A0_07460"
     CDS             complement(1659..2606)
                     /locus_tag="AC16A0_07460"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3105208.1"
                     /translation="MDSIFNTVLPVFGMIVLGYGFTRICVFNAAAGKGITLFVFSVAI
                     PALLFKTVAAMEPQSGSPWGLWLAFFGGLAITWMVAAFVSRFFDGLNVSGGASTSMAA
                     GFGNLALLGTPLALAHFGQNAVIPLGMILSIHAPVLWFAAMLHREIARHSGSFSMKRT
                     ARELVVNLATNAIVLALVAGSLWRSTGFALHPIAVTMLDMLADASVPTALFALGVSLA
                     GYSLKGAWSGMFAIIGLKMVLMPVLVFLACRYAVTLPPFWVKIAVLFAAMPTGANAFL
                     FSQRNEEAVAEVSGAIALGTGLAAISASVLLYLMDTNVI"
     gene            complement(2610..3215)
                     /gene="leuD"
                     /locus_tag="AC16A0_07465"
     CDS             complement(2610..3215)
                     /gene="leuD"
                     /locus_tag="AC16A0_07465"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3105209.1"
                     /translation="MQKFETLTGVAAPLNIVNIDTDMIIPKQFLKTIKRTGLGKSLFF
                     EMRYTQDGKEIADFVLNQPAYRKAEILVAGDNFGCGSSREHAPWALLDFGIRCVISTS
                     FADIFYNNCFKNGILPIKVSPEDLKKLMDDAERGSNATLTIDLPKQEIRGPDGGVVTF
                     DLDPFRKHCLLNGLDDIGLTMEKSASIDSYEKKQSAAQPWL"
     gene            complement(3225..>3497)
                     /locus_tag="AC16A0_07470"
     CDS             complement(3225..>3497)
                     /locus_tag="AC16A0_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105210.1"
                     /translation="DCLAEAYDGSPVIEVAPFAEIERSESISPEVLNNTNRMRLHVFS
                     NELRGQLLLMAIYDNLGKGASGAAVQNLNIMIGAEETLGTDLTQAS"
BASE COUNT          696 a         1065 c         1139 g          597 t
ORIGIN      
        1 gatccggtcg gctagctctc gtcggagttc tcggttcgat catcctccgc ttcgaccgca
       61 ggaatcgcat agtgaccgcc gagccaacgg ctgagatcga tctgggcgca tcgattcgag
      121 cagaagggat ggaacttctg ctgcgacggc ttgctgcaga tcggacacgg ccgcttcggc
      181 ctcaggcgga ccggatctgt catggcgctt cagcgccggc tcccgtcatc cagctgtgaa
      241 acaccggata gcccgcgcct tcgagaaggt ggaccgtctc gtagagcggc agcccgacaa
      301 cggccgaatg tgatccggtc agcatgcgca cgaaggcctc cgcacggccc tggatagcat
      361 aggcgccggc cttgccgcgc cattcccccg atgcgatgta gatgtcgata tcctcgcgcg
      421 acaggcgctt gaagcgaacc ttggtctcga cacagcgcct cgacgtgcgg cggcccggcg
      481 ccaccagcac cacggtcgaa tagacccaat gcgatcgccc cgagagcagc gacaggcagt
      541 cgcgtgcttc gtcctcggtc tccgctttcg ggatgattct gcgcccaagc ccgacgacgg
      601 tatcggcggc aagcaggaag atgttcggtc cgagcgcgcg cacctgcggc atatccagcg
      661 ccaccgtagc cttttcccca gcgaggcgga tcgaatgcgc gcgcggtgtc tcgcgcttgc
      721 ggggcgtctc gtcgatatcg gccggattga gaaggtcggg aacgagtcct atgcgctcga
      781 gcagttgcaa ccgccgcggc gaggcgctgg ccagaacgag cttgacggga ctctcggcca
      841 tcgggtgcta cttgaacctg tagttgatcc ggcccttcgt cagatcgtag ggcgtcattt
      901 cgaccatgac acggtcgccg gccaggacac ggatgcggtt cttgcgcatc tttccggcgg
      961 tgtgggcaat gatttcatgg ttgttgtcga gcttcacgcg gaacgtcgcg ttgggcagaa
     1021 gttcggtaac ggtgccttcg aattccagca gttcttcctt ggccatgtga ctccttgaag
     1081 gtttaaagcc gcgcggaaaa tggacccatt gccgtccaaa atcaagcacg ctccgggggc
     1141 ttggcgggat aggtcgtgcc ggtcagccgg tgataggccg tgcccagcag gaccagcgcc
     1201 acggcaccgg agaggatggg aaatgcaagg aaggctgcac tctgtgcacc agcataggag
     1261 accaaggcgg ttgcccccgc cggcggatgg gtaacgcgga aaatcgccat tccggtgatg
     1321 gccaggccca cggcgacggc agcgatccag acactgtccg gcagggccag atgggcgccg
     1381 accccgatga ccccggccag cgcatgaccg aggacgacgt tcacgggttg cgacagtggg
     1441 ctggccggca ccccgaagac aaggacgcag gtggcgccca acggtgcgaa gagcatggtc
     1501 aggcctgcca ggtcttgcgt gagcccgatg gctgcaaccg ccagcatggc cccaagaccg
     1561 ccgaggatgg ctgttctgag cggagacggc ggctggtggc ggaaggtaaa gtgacgcatg
     1621 cagcccctta cacgcgggag gctgacgctg caaagggatc agatgacatt ggtgtccatg
     1681 agatagagca gcaccgaggc ggaaatcgcc gcaagtcccg tacccaatgc aatggcgccc
     1741 gagacttcgg ccacagcctc ctcgttgcgc tgcgagaaga gaaaggcatt ggcccccgtc
     1801 ggcatcgcgg cgaagagcac cgcgatcttc acccagaagg gcggcagcgt cacggcatag
     1861 cggcaggcca ggaacaccag cacgggcatc agcaccatct tgaggccgat gatcgcgaac
     1921 atgccgctcc aggcaccctt cagcgaatag ccggccagcg acacccccag cgcgaacagc
     1981 gccgtcggga cactggcatc ggccagcatg tcgagcatgg tgacggcgat cgggtgaagc
     2041 gcgaagccgg tactacgcca caggctgccg gcgaccaggg ccagcacgat ggcattggtc
     2101 gcgagattga ccaccagctc gcgcgcggtg cgcttcatgg aaaaggatcc ggagtgccgc
     2161 gcgatctcgc gatgaagcat ggccgcaaac cacaggactg gcgcgtggat cgacaggatc
     2221 atgcccagcg ggatcactgc attctggccg aaatgcgcca gcgcgagcgg cgtgcccagc
     2281 agcgccagat tgccgaagcc ggccgccatc gacgtcgaag cgccgccgct gacattcagg
     2341 ccgtcaaaga accgcgacac gaaggccgca accatccagg tgatggcgag cccgccgaag
     2401 aaggcaagcc acaggcccca gggggaaccg ctctgcggct ccatggcggc cactgtcttg
     2461 aaaagcagcg ccggaatggc gacactgaag acgaagagcg tgatgccctt gccggcagct
     2521 gcgttgaaaa cgcagatacg cgtgaagcca tagccgagca cgatcatgcc gaagaccggc
     2581 aggaccgtat tgaagatcga gtccaaggcc tagagccagg gctgggcggc ggactgtttc
     2641 ttttcgtagc tgtcgatcga cgcggacttc tccatggtca ggccgatgtc gtccagtcca
     2701 ttcagcaggc agtgcttgcg gaaaggatcg agatcgaacg ttaccacgcc gccatcgggg
     2761 ccacggattt cctgctttgg cagatcgatg gtgagcgtgg cattcgatcc ccgctcggca
     2821 tcgtccatca gcttcttcag gtcctcgggc gaaaccttga tcggcaggat gccgttcttg
     2881 aagcagttgt tgtagaagat gtcggcgaag ctcgtcgaga tcacgcagcg gatgccgaag
     2941 tcaagcaggg cccagggcgc atgttcgcgc gacgagccgc agccgaagtt gtcgccagcc
     3001 acgaggatct cggcctttcg gtaggccggc tggttgagca cgaaatcggc gatctccttg
     3061 ccatcctggg tgtagcgcat ctcgaagaac agcgacttgc caaggcccgt ccgcttgatg
     3121 gtcttgagaa actgcttggg gatgatcatg tcggtgtcga tgttcacgat gttgagcggc
     3181 gctgccacac ctgtgagggt ttcgaacttc tgcatgggac ctctctagga tgcctgggtg
     3241 agatcggtac cgagcgtttc ctccgcaccg atcatgatgt tgagattctg tacggccgct
     3301 ccggaagcgc ccttgccgag attgtcgtag atggccatca gcagaagttg cccccgcagc
     3361 tcgttgctga acacgtgcag ccgcatgcgg ttggtgttgt tgagcacctc ggggctgatc
     3421 gactccgagc gctcgatctc ggcaaagggc gcaacctcga tgacgggcga tccatcatag
     3481 gcctcggcca ggcagtc
//
LOCUS       DBNGVR010000351         4653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1898644, whole genome shotgun sequence.
ACCESSION   DBNGVR010000351 DBNGVR010000000
VERSION     DBNGVR010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1898644"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="AC16A0_07475"
     CDS             complement(<1..178)
                     /locus_tag="AC16A0_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3105211.1"
                     /translation="MAYPAPFVGRIQKVEDQWTDYNGHFNMAYYNVLFDRAGDEAFAA
                     VGLGPDYVKDRNASF"
     gene            242..1603
                     /locus_tag="AC16A0_07480"
     CDS             242..1603
                     /locus_tag="AC16A0_07480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3105212.1"
                     /translation="MQAVAEVREVLGDRLSTAAAVREQHGKDQTWNPGAPPDAVAFVQ
                     NTGEVQRIVAICGKYDTPVIAYGTGTSLEGHFTAPFGGISIDLSGMNRILEVNAADLD
                     CRVEAGVTRKQLNVHLRDQGLFFPVDPGADASLGGMTATRASGTNAVRYGTMRENVLN
                     VTAVMANGEIIRTGSRAKKSSAGYDLTRLLVGSEGTLGVITEVALRLYGIPESIVAAV
                     CPFPSVEACCNATITAIQMGLPVARIELVDAEHVKAFNAYSHLDLAVSPTLFLEFHGT
                     VASAREQAETFAAIAEDLGGGPYRWAEKEEDRQKLWQARHDAFWATRSLMPGKDAFAT
                     DVCVPISRLAECVTDTQADLAAHGLYGPIVGHVGDGNFHVVLYCDRSDPDEVARVKGV
                     YGRLIDRAIAMGGTCTGEHGIGSGKVAYLEREHGPALAFMRDIKRALDPKNIMNPGKN
                     AAL"
     gene            1641..>4653
                     /locus_tag="AC16A0_07485"
     CDS             1641..>4653
                     /locus_tag="AC16A0_07485"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3105213.1"
                     /translation="MWKSPVLYFGIFLVLAVLGLLAAPFVIDWNSYRADIESNGRKLT
                     GRTVTVDGNVSARLFPWPRLTVEGLRIANPAGMENAEFATVDRAVIRMSLAGLMSGNI
                     EVQSIEVERPVITVERRDGGDGNWVFTPSESVVNSDMLRKVRLDRITVTDGTLRFVDR
                     RRGETYELTDVNAALSASAIAGPWRLRALDGRKSVARYGDRDFDLSLTTAAWIEGEPL
                     RLGLRVSPADAAGPIFSFDGRVQAGKAEGEVRVAPAETPEGKTNSEGQLRPLVFTSQV
                     TADFDGAAFDRIEISPVNIQEGGTLVSGTARVALDRHISASADLSAVSLDLDELAGAR
                     ARNLLREGGVLSLAGGLLALIPEDVSLSAAMRVTSLTIGGERLDNAAVVVDADRNAIR
                     LKELSTSLPGRSRVLYEGVFFPGTAGAEVAGSLALESGDLRQLSAWIWPEAKPSIERL
                     WTGSRGQFKMQTDLNITPSRLRFSKTEYELDGERGTAELTLTSGGRTAVDLRLDAGRL
                     DFDGLSGGSLSALAGAGTFDLAGMIGRLVPRGDAADVKLALKARELRLNGVTASDVAL
                     DLASGVTGLDLRTLEIGSVGGARLKATGLILGGNAGPDGSIGVEIQAEDPRELLRLAG
                     LIKGESDPAWAAELGRTALKGTVAVRPKDGGIETGFDVNGTAGDISLAITGQIDGGAT
                     PEATRISGSSEIGTGSSARMAALVGLAPVGDDQQPARLVATFDGTLGQGFRSDLQLDA
                     FGGRHAYRGQINPFVPGLGLDGKLEMRATDVGAMARAMGLPLQSVPSGVLVIDAPVKT
                     EGGEIRVADLSGRLGESALKGNLSLSPGGIVKADLQTGPMHLADLLSAAFLEWRGAAP
                     NRESSFSVGLPLGLTGEFWITPSRLTLHEHLVADGALVGIVATGSETRFTLRNKDGEG
                     RDTKLEIGAAGGSGTRTIDGSLTLPVNLGTDLVRADGGSVAEGQGAITLSFKGEGRSP
                     AGVLAALTGAGSYSFDDVAVAGLEPDAFLAAV"
BASE COUNT          758 a         1532 c         1569 g          794 t
ORIGIN      
        1 agaacgaggc gttgcggtcc ttcacgtagt ccgggccgag gcccacggcc gcgaaggcct
       61 cgtcgcccgc ccggtcgaac agcacattgt agtaggccat gttgaaatgg ccgttatagt
      121 cggtccattg gtcctcgacc ttctggattc ggcccacgaa cggggcggga taggccatcg
      181 caaattctcc ggattctgct agtttcgggg catggataca cgaagcgcca agaacagcac
      241 aatgcaggcc gtcgccgagg tccgcgaggt cctgggcgac cgcctgtcga ccgccgccgc
      301 cgtgcgcgaa cagcacggca aggatcagac ctggaatccg ggtgcgccgc ccgacgcggt
      361 ggccttcgtg cagaataccg gcgaagtcca gaggatcgtt gccatctgcg gcaaatacga
      421 cacgcccgtg attgcctatg gcaccggcac ctcgctggag gggcatttca ccgcaccctt
      481 cggcggcatt tccatcgacc tgtccggcat gaaccgcatt ctggaagtga atgccgccga
      541 tctcgactgc cgggtggaag ccggggtcac ccgcaagcag ctcaatgtcc atttgcggga
      601 ccagggtctg tttttccccg tcgatcccgg cgccgatgcc agtcttggcg gcatgacggc
      661 cacccgggcc tccggaacca acgccgtgcg ctacggcacc atgcgcgaga acgtgctgaa
      721 cgtgaccgca gtcatggcca atggcgaaat catccgcacc ggatcgcgcg ccaagaagtc
      781 ctcggcaggc tacgatctca cccgcctgct ggtgggcagc gaaggaacgc ttggcgtcat
      841 caccgaagtg gcactcagac tctacggcat tccggaatcg attgtcgcgg ccgtgtgccc
      901 gtttcccagc gtcgaagcct gctgcaatgc caccatcacc gccattcaga tgggactgcc
      961 ggtggcgcgt atcgaactgg tcgacgccga gcatgtgaag gccttcaacg cctactcgca
     1021 tctcgatctg gccgtctcgc ccacgctgtt cctcgagttc cacgggaccg tcgcctccgc
     1081 gagggagcag gccgaaacct tcgcggcaat tgccgaagac ctcggcggcg ggccctatcg
     1141 ctgggccgag aaggaagaag accggcagaa gctctggcag gcccggcacg atgccttctg
     1201 ggccaccagg tcgctgatgc ccggcaagga tgcctttgcc accgacgtct gcgtgcccat
     1261 ctcgcggctt gccgaatgcg tgaccgacac ccaggccgat ctcgcggcgc atggcctcta
     1321 tggcccgatc gtaggtcacg tgggcgacgg caacttccat gtggtgctct attgcgaccg
     1381 ttcggatccg gatgaggtgg cgcgggtcaa gggcgtctac gggcggctga tcgaccgggc
     1441 catcgccatg ggcggcacct gcaccggcga gcacggcatc ggttccggca aggtggccta
     1501 tctggagcgc gaacatggcc cggcccttgc tttcatgcgc gacatcaagc gcgcgctcga
     1561 tcccaagaac atcatgaacc ccggcaagaa cgccgccttg tgattgacgc aatggaaccg
     1621 catctgacag ggccatgaag gtgtggaaat cccccgtcct ctatttcggc attttcctgg
     1681 tgcttgccgt ccttggcttg ctggctgcgc ccttcgtcat cgactggaat tcctatcgcg
     1741 ccgacatcga gagcaatggc cgcaagctga ctggccgcac cgtcaccgtc gatggcaatg
     1801 tctcggcccg gctcttcccc tggccgcggc tgacggtgga aggccttcgc atcgccaatc
     1861 ccgccggcat ggagaatgcc gagttcgcca ccgtcgaccg cgcagtgatc cgcatgtcgc
     1921 tggcggggct gatgagcggc aacatcgaag tccaatccat cgaggtcgaa cggccggtga
     1981 tcacagtgga gcggcgcgac gggggtgacg gcaactgggt ctttacgccg tcggaaagcg
     2041 tcgtgaacag cgacatgctg cgcaaggtcc ggctcgaccg gatcaccgtg acggatggca
     2101 cgctgcggtt cgtcgaccgc cgccgtggcg agacctacga actgacggat gtgaatgcgg
     2161 cactttcggc atcggccatt gccggaccct ggcggctgcg cgcgctcgat ggccgcaaga
     2221 gtgtcgcccg ctacggggac agggacttcg acctgtcgct caccaccgct gcatggatcg
     2281 agggtgagcc gctgcgcctg ggcctgcgcg tctcgcctgc cgatgcggcg ggtccgatct
     2341 tctcctttga cggccgtgtc caggcaggca aggccgaagg cgaggtgcgc gtggcgcccg
     2401 ccgaaacgcc cgagggcaag accaattcgg aaggtcagct ccgcccgctg gtctttacct
     2461 cccaggtgac tgccgacttc gacggcgctg ccttcgaccg gatcgagatc tctccggtca
     2521 atatccagga aggcggtacg ctggtgagcg gtacggcgcg tgtcgcgctc gaccggcaca
     2581 tttcggcaag tgccgatctc tcggcggtct cgctcgatct cgacgagctt gccggcgcgc
     2641 gcgcgcgcaa tctgctgcgc gagggcggcg tgctgtcgtt ggcgggcggt ctgctggcgc
     2701 tcattccgga agacgtgagc ctctcggcag ccatgcgcgt gacctcgctg accattggcg
     2761 gcgagcgcct ggacaatgcc gccgtcgtgg tcgatgccga ccggaatgcg atcaggctga
     2821 aggaactgtc gacatcgctg ccgggccgct cccgggtcct ttacgaaggc gtgttctttc
     2881 cgggcactgc cggggccgaa gtcgccgggt cgttggcact cgagtcgggt gacctgcggc
     2941 aattgtcggc ctggatctgg ccagaagcga aaccgtcgat cgagcgcctg tggaccggca
     3001 gccgcggcca gttcaagatg cagacggacc tcaatatcac gccctcgcgc ctgcgcttca
     3061 gcaagacgga gtacgaactc gatggcgagc gcggcaccgc cgagctcacc ctcacgtcgg
     3121 gcggccgcac cgcggtcgac cttcggctcg acgcagggcg gctcgacttc gacggcctgt
     3181 cgggcggtag cctctcggcc ctggccggtg caggcacctt cgacctcgcg ggtatgattg
     3241 gccgccttgt gccgcgcggc gatgcggccg acgtcaagct ggcgctcaag gcgcgggagc
     3301 tgcggctcaa cggcgtcacc gcatcggatg tcgcgctcga tctcgcgtcg ggcgtgaccg
     3361 gactcgatct ccggactctc gagatcggtt cggtgggtgg cgcccggctg aaagccaccg
     3421 ggctgatcct tggcggcaat gccggcccgg acggatcgat cggcgtcgag atccaggcgg
     3481 aggacccgcg cgagctgctg cgccttgccg gactgatcaa gggagaatcc gatccggcct
     3541 gggccgcgga gcttggccgc acggcgctga agggcacggt cgccgtgcgc ccgaaggacg
     3601 gcggcatcga aaccgggttc gatgtcaacg gaacggcggg cgacatcagc cttgccatca
     3661 cgggccagat cgatggcggc gcaacgccgg aggcaacgcg catcagtggg tcgagcgaga
     3721 tcggcaccgg cagcagtgcg cggatggcgg cactcgtcgg tctcgccccg gtcggcgatg
     3781 atcagcaacc ggcgcgtctg gtggcgacct tcgacggaac gctgggccag ggcttccgca
     3841 gcgatctgca actcgacgcc tttggcgggc gacatgccta tcgcggccag atcaatccct
     3901 ttgtccccgg actgggcctc gacggcaaac tggagatgcg ggccaccgat gtgggcgcca
     3961 tggcaagggc gatgggcctg ccgcttcagt ccgtgccgtc cggcgtgctg gtgatcgatg
     4021 cacccgtcaa gaccgaaggt ggggagatcc gggttgcgga cctctccggc cggctgggcg
     4081 agtcggccct caaggggaac ctcagccttt cccccggcgg gatcgtcaag gccgatctcc
     4141 agaccggtcc gatgcatctt gccgacctgc tgtctgcggc cttccttgag tggcgcggag
     4201 cagcgcccaa ccgcgagtcc agcttctccg tcggattgcc gctggggctc accggcgagt
     4261 tctggatcac gccgtcccgc ctcacgcttc atgaacatct cgtggccgat ggcgcgctgg
     4321 tcggcatcgt ggcgaccggg tcggagaccc gcttcacgct gcgcaacaag gatggcgagg
     4381 gccgcgacac caagctggag atcggggcgg ccggcggcag tggcacccgc accatcgacg
     4441 ggagcctcac cctgccggtc aatctgggca ccgatctcgt gcgggctgac ggcggcagcg
     4501 tggccgaagg tcagggtgct atcacgctct cctttaaggg cgagggccgc agcccggccg
     4561 gcgttctggc ggccctgacc ggtgcgggca gctacagctt cgatgatgtg gcggttgcgg
     4621 ggctggagcc tgacgcgttc ctcgccgccg tcg
//
LOCUS       DBNGVR010000352         2713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4844744, whole genome shotgun sequence.
ACCESSION   DBNGVR010000352 DBNGVR010000000
VERSION     DBNGVR010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4844744"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="AC16A0_07490"
     CDS             <1..526
                     /locus_tag="AC16A0_07490"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3105214.1"
                     /translation="ACCHGTHAMIEALQKLMTQRAIAPDAIASIEVRINPRWLKVCDI
                     KAPRSGLEIKFSYAYLAAMVLSGIDTAADHSYADAVCDSAALRQLSQRVTVVGDAHIG
                     DMETEVALMLPDGLRVAGHHDLSVRHAPEVIANGLRRKARALLGEERAEAIWASIAGI
                     DAFTARDLAALLRG"
     gene            complement(528..965)
                     /locus_tag="AC16A0_07495"
     CDS             complement(528..965)
                     /locus_tag="AC16A0_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HIR3105215.1"
                     /translation="MSNRMPSLAALLGLVAIAGYQNREKIGEFVKGLTGKDPAVAAGD
                     AVGKVKEVLGDGPIATKITGGLGELVDHFKTTGHGETADSWVSTGPNRPITETSLESA
                     LGGDLIDSLVRQTGLTRQELLARLSKILPEAVDRMTPEGKIPG"
     gene            1093..1350
                     /locus_tag="AC16A0_07500"
     CDS             1093..1350
                     /locus_tag="AC16A0_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3105216.1"
                     /translation="MGIIWTIIIGFVVGVIAKFLKPGNNEPSGFILTTILGIVGAFVA
                     SYLGQAVGIYQAGQGAGFIGAIVGAVIVLWIWGMLTKNKTV"
     gene            1468..1767
                     /locus_tag="AC16A0_07505"
     CDS             1468..1767
                     /locus_tag="AC16A0_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HIR3105217.1"
                     /translation="MANLIITYWRDIPSAVSVKAGRKEEKRMLDNRFMEAIDMAAMRG
                     GATNTDEYLADWRRSDPVPVGDDLAAEVEKAKAELEARFTQDVIKTLIANGGKAQ"
     gene            1764..>2713
                     /locus_tag="AC16A0_07510"
     CDS             1764..>2713
                     /locus_tag="AC16A0_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HIR3105218.1"
                     /translation="MTHTLVASATREHIIGFDRPFTVIGERINPTGRKKLAAEMQAGN
                     FETVIKDAMAQVAAGAHILDVNAGVTAVNPNESEPPLMRQTLEIVQSITDIPLCIDSS
                     VTSALKAGLETAKGRPLVNSVTGEEEKLEAILPLVAKYNVAVVAISNDETGISEDPDV
                     RFAVAKKIVERAADHGIKPHDIVVDPLVMPIGAMGTAGQQVFRLVRRLREELKVNTTC
                     GASNISFGMPNRRALTGYFLAMAASHGMTSAIMNPLHDEDMHAIFGANVLNGTDRHCK
                     FWTQKFREMTAGPSAAAQAAGEVSSNEDIEARRKAREARR"
BASE COUNT          510 a          887 c          854 g          462 t
ORIGIN      
        1 tgcctgctgc catggaaccc atgcgatgat cgaagccttg cagaagctca tgacgcagcg
       61 cgcaatcgca cccgatgcga ttgcaagcat cgaggtgcgc atcaatccgc gctggctgaa
      121 ggtctgcgac atcaaggctc cgcgcagcgg gctggagatc aagttcagct atgcctacct
      181 cgccgcgatg gtcctgagcg gtatcgacac ggcggcggat cattcctatg ccgatgcggt
      241 gtgcgacagc gcggcgctgc gtcagctgtc gcagcgtgtc accgtggtgg gcgatgcgca
      301 catcggcgat atggaaaccg aagtcgcgct aatgctgccg gacgggctcc gcgtggccgg
      361 gcaccacgat ctgtccgtcc gccacgctcc cgaggtcatc gccaatgggc tccgccgcaa
      421 ggcaagggcc ctgctgggcg aagagcgggc ggaggcgatc tgggcaagca ttgccgggat
      481 cgacgcattc accgcgcgcg atcttgcggc tctgttgcgc ggctagctca gccggggatc
      541 ttgccctcgg gcgtcatgcg gtcgaccgcc tcgggcagga tcttggagag ccgcgccaga
      601 agctcctgcc gggtgaggcc ggtctgccgg accaggctgt cgatgagatc gcccccaaga
      661 gccgattcga gacttgtttc ggtaatcggc ctgttgggcc cggtcgagac ccaggagtcg
      721 gccgtctccc cgtgcccggt ggtcttgaaa tggtccacca gctcgcccaa tccgcccgtg
      781 atcttcgtgg cgatggggcc gtcgcccaga acctccttga ccttgccgac ggcatcgccg
      841 gcagcaaccg cggggtcctt cccggtcagg cccttgacga attcgccgat cttctcgcgg
      901 ttctggtaac cggcgatggc aacgagaccc agcagggcgg caagcgatgg catacgattg
      961 gacatgatac tctcccgatt ggctctggat gacactcatt ctgtcacttg gctgggcaaa
     1021 tgcaacccgc ccgcgttgat tgtacctgcc caagaatggt aaacatatgg cgtcgcagat
     1081 caggggaaca tcatgggtat catctggacc atcatcatcg ggtttgtcgt gggagtgatc
     1141 gccaagttcc tgaagccagg gaacaacgaa ccgtcaggtt tcatcctgac caccatcctg
     1201 ggcatcgtcg gcgccttcgt cgcctcctat ctgggtcagg ccgtcggaat ctatcaggcc
     1261 ggccagggcg cggggttcat cggggccatc gtgggcgccg tcatcgtgct ctggatctgg
     1321 ggaatgctca ccaagaacaa gaccgtctga cttccggcct tccggcctgc acagcggcgg
     1381 acccccggtc cgccgccgat ccgaccgtgt tgcatgtcgt tttcaggcaa ttctccctga
     1441 cccgaaacgg cgataaagca gcccatcatg gcaaacctga tcatcaccta ctggcgcgac
     1501 attccctctg ccgtctcggt caaggccggg cggaaggaag aaaagcgcat gctggacaac
     1561 cgcttcatgg aggccatcga catggccgcg atgcgcggcg gggccaccaa caccgacgag
     1621 tacctggctg actggcggcg atcggacccg gtgccggtcg gcgatgatct ggctgccgag
     1681 gtcgagaagg ccaaggccga actcgaggcc cgcttcaccc aggatgtcat caagaccctc
     1741 attgccaatg gaggcaaagc ccaatgaccc acaccctcgt cgcctccgcg acccgcgagc
     1801 atatcatcgg cttcgaccgg cccttcacgg tgatcggcga gcgcatcaat cccacgggcc
     1861 gaaagaaact ggccgccgag atgcaggccg gcaatttcga gacggtgatc aaggacgcca
     1921 tggcccaggt ggcggccggc gcgcatatcc tcgacgtcaa tgccggcgtg accgcggtca
     1981 accccaatga gtcagagccg ccgctgatgc ggcagacgct cgagatcgtg cagtcgatca
     2041 ccgacatccc gctgtgcatc gattcctccg tcacctcggc gctgaaggcc gggctcgaga
     2101 cggccaaggg ccggccgctg gtcaattcgg taaccggcga ggaagagaag ctcgaggcca
     2161 tcctgccgct ggtggcgaag tacaatgtcg ccgttgtggc catctccaac gacgagaccg
     2221 gcatctcgga agacccggac gtgcgtttcg cggtggccaa gaagatcgtc gagcgcgccg
     2281 ccgatcacgg catcaagccg catgacatcg tggtcgaccc actcgtcatg ccgatcggcg
     2341 ccatgggcac ggctggccag caggtgttcc gcctcgtgcg ccgcctgcgc gaggagctga
     2401 aggtgaacac aacctgcggc gcctcgaata tctccttcgg catgccgaac cgccgggccc
     2461 tgaccggcta cttccttgcc atggcggcaa gccacggcat gacctcagcc atcatgaacc
     2521 cgctgcatga cgaagacatg catgccatct tcggcgccaa cgtgctgaac ggcaccgacc
     2581 gccattgcaa gttctggacg cagaagttcc gcgagatgac cgcaggtccg tccgccgccg
     2641 cgcaggccgc gggcgaagtc tcgtcgaacg aggacatcga agcccggcgc aaggcgcgcg
     2701 aggcccggcg gag
//
LOCUS       DBNGVR010000353         2857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1898958, whole genome shotgun sequence.
ACCESSION   DBNGVR010000353 DBNGVR010000000
VERSION     DBNGVR010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1898958"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="AC16A0_07515"
     CDS             <1..1129
                     /locus_tag="AC16A0_07515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3105219.1"
                     /translation="EYKARIKAVTQALEARGLDGLLVFQQESMYYLTGYDTFGFCFFQ
                     CLYLRSDGKLALLTRSADLRQAQHTSIISDIRIWTDEAGAKPAGQLREMLASLGAKGK
                     KLGIETNSYGLTHYNGRAVDEALSGFCELVEATDVVNMIRVVKSPAELKYVRKAGALG
                     DAALKAAIRKTKAGADEGDILAAQQGAIFAGGGDYPANEFIIGSARDALLCRYKSGRR
                     KLSKRDQLTLEWAGVYRHYHAAFMRTLIIGKPSKVHLAMPAAAREALAEVETQLRPGR
                     TAGDVFDAHARVMDAHGMQRHRLNACGYSLGAKFTPSWMDSPMFYRGNPTVIGPGMVF
                     FAHMILMNSDAGAAYCLGRTYIIGEKGPEPVSRYPLEMVIR"
     gene            1177..2280
                     /locus_tag="AC16A0_07520"
     CDS             1177..2280
                     /locus_tag="AC16A0_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105220.1"
                     /translation="MNRYAALLAVLLVLVACGSKPSFEPVDGSAKNPGKPVPAVALRE
                     VTGVPPGKLNELSTALAVAAGQRDIGIVEGTALADGTFSLTGAFRAATAAGGVTVNYQ
                     WQLRDSAGVLVHTINGDESAGLFTGADVWAAVTPTVWERIARSTAQSMATRMAQLGYA
                     TRVSALAAPPADHFVRAGPGAEREIDMALLTGSRQTSAAFDGLEAQSRLAQSATATIP
                     NDATPAAAPADLAAAEPEASPDEPVIDPEGKVRIRAVAVLPVQGSGKGDKELTAAMRR
                     TLSEAGWPVVSRPQPDAITVIGRVKISEAKNGSQDVTVRWEVQTPAGSELGDVKQANQ
                     IPAGALDNGWGGAALAVAQAAAPGIYDIVKRFQ"
     gene            2391..>2857
                     /gene="prs"
                     /locus_tag="AC16A0_07525"
     CDS             2391..>2857
                     /gene="prs"
                     /locus_tag="AC16A0_07525"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3105221.1"
                     /translation="MKIISGNSNRPLAEGICSYLNLPITKAIVKRFNDMEVFVEIQEN
                     VRGQDMFVVQSTSFPANDHLMELLIIIDALKRASAKRITAVIPYFGYARQDRKSGSRT
                     PISAKLVANLITHAGADRVLTMDLHAGQIQGFFDIPTDNLFSGPVFVRDINQH"
BASE COUNT          510 a          926 c          931 g          490 t
ORIGIN      
        1 ggaatacaag gcccgcatca aggccgtcac ccaggcgttg gaggcccgtg gcctggacgg
       61 cctgctggtg ttccagcagg agagcatgta ctatctcacc ggctatgaca cattcggatt
      121 ctgcttcttc cagtgcctct atctccgcag cgacggcaag ctggcgctgc tgacacggtc
      181 ggctgacctg cgccaggcgc agcacacctc catcatatcc gacatccgta tctggaccga
      241 tgaggccggc gcgaagccgg caggccagct gcgcgagatg ctcgcaagcc tcggtgccaa
      301 gggcaagaag ctgggcatcg aaaccaattc ctacgggctc acccactaca acggcagggc
      361 agttgacgag gcactttccg gattctgcga gttggtggaa gcaaccgatg tcgtgaacat
      421 gatccgcgtg gtgaaatcgc ccgcagaact gaagtatgtg cgcaaggccg gggcacttgg
      481 cgatgcggcg ctcaaggcgg ccatccgcaa gacgaaggcc ggcgccgatg agggtgacat
      541 ccttgccgcg cagcagggcg cgatcttcgc gggcggcggc gactatcccg ccaatgaatt
      601 catcattggc tcggcaaggg atgccctgtt gtgccgctac aagtcgggcc gccgcaaact
      661 gagcaagcgc gaccagctca cgctcgagtg ggcgggcgtc taccgtcact accacgcggc
      721 cttcatgcgc acgctgatca tcggcaagcc gagcaaggtc cacctggcca tgcccgcggc
      781 agcgcgcgag gcgctcgccg aggtggagac gcagctgcgg ccgggccgca ccgccgggga
      841 tgtcttcgat gcccatgcgc gggtgatgga cgcccatggc atgcagcggc accgcctgaa
      901 tgcctgcggc tattccctgg gcgcgaaatt caccccgtcc tggatggata gccccatgtt
      961 ctaccggggc aatccgacgg tcatcggccc gggcatggtg ttcttcgccc acatgatcct
     1021 gatgaattcc gacgccggcg cggcctattg ccttggaagg acctatatca ttggggaaaa
     1081 gggaccagag ccggtttcgc gttatccgct ggagatggta attcgttaga catgcggttc
     1141 agatttggcg tgagcttgcc gggaggccgc cggcttgtga atcgttatgc agcactcctg
     1201 gcggtgctgc tggttcttgt tgcatgcggc agcaagcctt ccttcgagcc ggtcgatggc
     1261 agcgccaaga accccggcaa gccggtcccg gccgtagcac tccgagaggt gacgggcgtc
     1321 ccgcccggca agctgaacga gctttcgact gccctcgcgg tggcggcagg gcaacgcgac
     1381 attggcatcg tcgagggcac cgcactcgct gatggaacct tttccctgac cggtgctttc
     1441 cgggccgcca ccgccgcggg aggcgtcacc gtcaactacc aatggcagct gcgcgattct
     1501 gcgggcgtgc tcgtgcatac catcaatggc gatgagtccg ccgggctgtt caccggcgcg
     1561 gatgtctggg cggccgtgac gccgacggtc tgggagcgca tcgcccggtc gaccgcccag
     1621 tcgatggcga cacgcatggc ccagctgggc tatgccaccc gggtttcggc actcgccgcg
     1681 cctccggccg accacttcgt gcgggcgggg ccgggggccg agcgggaaat cgacatggcg
     1741 ctgctcacgg gctcgcgcca gacctcggcc gctttcgacg gcctggaagc ccagtcgcgg
     1801 ctggcccagt cggcgacggc gaccattcca aacgacgcga cgccggctgc cgcccccgcc
     1861 gatctcgcag cggcggagcc tgaggcgagt cccgatgaac cggtcatcga tccggaagga
     1921 aaggtccgca tcagggcagt cgccgtcctg ccggtccagg gttcgggcaa gggcgacaag
     1981 gaactgacgg ccgcgatgcg gcggactctc tccgaggcag gctggccggt cgtctcgcgg
     2041 ccccagccgg atgccatcac ggtgatcggc agggtcaaga tctccgaggc caagaacgga
     2101 tctcaggatg tgactgtccg ctgggaagtc cagacgcccg ccggcagcga attgggcgac
     2161 gtgaagcagg ccaaccagat cccggcgggc gccctcgaca atggctgggg tggggctgca
     2221 ctggcggtcg cccaggctgc cgcgccaggc atctacgaca tcgtcaagcg cttccagtag
     2281 cggcctccgg ggggcggcca tgtgtggaca ggaccatttg gcgctttgca tccgacctgt
     2341 gcggcggtta tagagcgccc atcagggctt ccgggggcgt accgcgcatg atgaagatca
     2401 tttcaggcaa cagcaaccgc ccgctggcag aggggatatg ttcctatctg aacctgccga
     2461 tcaccaaggc catcgtgaag cgcttcaacg acatggaagt cttcgtcgag atccaggaga
     2521 acgtgcgcgg ccaggacatg ttcgtggttc agtcgacctc ctttccggcc aacgaccatc
     2581 tgatggagct tctcatcatc atcgatgcgc tgaagcgggc ctcggccaag cggatcaccg
     2641 cggtgatccc ctatttcggc tacgcccggc aggaccgcaa gtcgggttcc cgcacgccga
     2701 tctctgccaa gctcgtggca aacctgatca cgcatgccgg cgccgaccgg gttctgacga
     2761 tggatctgca tgccgggcag attcagggct tcttcgacat tcccaccgac aaccttttct
     2821 caggcccggt cttcgtccgc gacatcaacc agcacta
//
LOCUS       DBNGVR010000354         2757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_522333, whole genome shotgun sequence.
ACCESSION   DBNGVR010000354 DBNGVR010000000
VERSION     DBNGVR010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_522333"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="AC16A0_07530"
     CDS             <1..738
                     /locus_tag="AC16A0_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3105222.1"
                     /translation="MISRLYQTKYGLPDSIVIDGDSLHRLNTKVPRMLFTMGNYIADR
                     YPVTSLPSPYTDHKVIFLARHPADTAVSFYFHLRNRVNPNLKDVKQVADAEGARDIFD
                     HLQNSPRGLRHVIQYMNDWAIALGHCPHNLLLRYEDLRADPVPQLRRIAAFLGDDFTE
                     SQFRDAADFASFAKLKEKERENFFATSKLQARDTANPDSFKVRRGKVGGYVDYLTPEQ
                     TAWVEATIARDLNPVFGYSSAGPTVAG"
     gene            complement(735..>2757)
                     /locus_tag="AC16A0_07535"
     CDS             complement(735..>2757)
                     /locus_tag="AC16A0_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3105223.1"
                     /translation="QPYQLKHLTLKNRLMSTSHEPNYAENGMPTDRYRLYHVEKARGG
                     IALTMTAGSAVVSRDSPPAFGNLLAYKDEIVPWLRKLADECHGHGAAVMIQLTHLGRR
                     TNWNKGDWLPVLSASPAREPAHRAFPKEMEDWDFDRIAADYAAAAERMKAAGLDGFEF
                     ECYGHLMDSFWSPATNRRTDEHNGSIDNRLRFTFRILEAVRKTVGPDFLVGCRLVADE
                     DWDRGLSRADGITICHKLKDSGLVDFLNIIKGHIEHDAPLADVIPVSGMKASPHLDFA
                     GEVRAATRFPVFHAARINDVATARHAIAEGKLDMVGMTRAHIADPHIVRKIAEGREGE
                     IRPCVGATYCLDRIYEGHEALCIHNPATGREATIPHVISRSTGPRRRIAVVGAGPAGL
                     EAARVSAERGHEVILFEAASQAGGQIRLATQLPRRRELIGIVDWRVAQCERLGVDMRY
                     NTIAEASDILALEADVVFIATGGLPRNEAIEEGAELAVSSWDVLSGDVKPAEDVLLFD
                     DNGAHPGMSAAERISDSGSRLEIVSPERFFAAEMGGLNLVPYMRNFTTRGVRITTMTR
                     VRALSRQGNKIKAVLFSPYTNADCGERLVDQVVVENGTSPLEDLYFALKDHAVNRGEV
                     DHGAMIDGRRQAIARNPAGRFQLFRIGDAVASRNIHAAIYEALRLAKDI"
BASE COUNT          501 a          857 c          875 g          524 t
ORIGIN      
        1 atgatctctc ggctgtatca gaccaagtat ggcctgccag actccatcgt tatcgacggt
       61 gacagcctgc acaggttgaa caccaaggtg ccgcgcatgc tgttcaccat gggcaactac
      121 atcgccgacc gttacccggt caccagcctt ccatcgccct atacggacca caaggtgatc
      181 tttctggcgc gacaccccgc cgatacggcg gtctccttct attttcatct caggaaccgg
      241 gtgaatccca acctcaagga tgtcaaacag gtcgccgatg ccgaaggtgc gcgcgatatc
      301 tttgaccact tgcaaaacag tccgcgcggg ctccgccacg tgatccagta catgaatgac
      361 tgggcgatcg cgcttggcca ttgcccacac aacctgttgc tgcgctacga ggatcttcgt
      421 gccgacccgg tcccgcaatt gcgccggatt gcagcctttc tcggagatga cttcaccgag
      481 tcccagtttc gtgatgcggc cgactttgca tccttcgcca agctaaagga aaaggagcgg
      541 gagaacttct tcgccacttc gaagctgcag gcgcgggaca cggccaatcc ggacagcttc
      601 aaggtgcggc gtggaaaggt cgggggctat gtcgactatc tgacgcccga acagacggcc
      661 tgggtggaag cgacaatcgc gcgggatctc aatcccgtct tcgggtacag cagtgctgga
      721 ccgacggtcg caggctagat gtcctttgcc agcctgagag cctcatatat ggcggcatgg
      781 atgttgcgcg aggcgacggc atcgccgatg cggaagagct ggaaccggcc tgccgggttc
      841 cgcgcgatcg cctgccggcg gccgtcgatc atggcgccgt ggtcgacctc tccgcggttg
      901 accgcgtggt ccttcagcgc aaagtagaga tcctccagcg gcgacgtgcc attctccacc
      961 acgacctggt cgaccagccg ttccccgcag tcggcgttgg tataggggct gaagagcact
     1021 gccttgatct tgttgccctg gcggctcaat gcccggacgc gcgtcatggt ggtgatgcga
     1081 actcctctgg tggtgaagtt ccgcatgtag ggcaccagat tgaggccgcc catctcggcg
     1141 gcgaagaagc gttccgggct gacgatctcg aggcggctgc cggagtcgga aatccgctcg
     1201 gccgcagaca tgccgggatg agcgccgttg tcatcgaaga gcagcacgtc ttctgccggc
     1261 ttcacgtcgc cggagaggac gtcccagctt gaaacggcaa gttcggctcc ttcctcgatg
     1321 gcttcattgc gcggcaggcc gccggtcgca atgaagacca catcggcttc gagtgcgagg
     1381 atgtccgatg cctccgcgat cgtgttgtag cgcatgtcga caccaaggcg ctcgcactgt
     1441 gccacccgcc agtccacgat gccgatgagc tcgcggcgac ggggcagctg ggtggcgagc
     1501 cggatctgtc cgccagcctg cgatgccgcc tcgaaaagga tgacctcgtg gccgcgttcg
     1561 gccgacaccc gcgccgcttc aagcccggcg ggacccgcgc cgacaacagc aatcctgcgc
     1621 cgcggcccgg tggatctcga aatgacatgc gggatcgtgg cttcgcgtcc ggtggccgga
     1681 ttgtggatgc agagcgcctc atgcccttca tagatgcgat ccaggcagta agtggcgccc
     1741 acacagggcc gaatctcgcc ttcgcggccc tcggcgatct tccgcacgat atgcggatcg
     1801 gcgatatggg cgcgcgtcat ccccaccatg tcgagcttgc cctcggcaat ggcatgacgc
     1861 gccgttgcca catcgttgat gcgcgcagca tggaagaccg gaaagcgcgt ggcggcccgt
     1921 acctcgcccg caaagtcgag atggggcgag gccttcatgc ccgacaccgg tatgacatca
     1981 gcgaggggtg cgtcgtgttc gatgtgaccc ttgatgatgt tgaggaagtc gacgagaccc
     2041 gagtccttca gcttgtgaca gatggtgatg ccatcggccc tgctgaggcc gcggtcccaa
     2101 tcctcgtcgg cgaccagccg gcatcccacc aggaagtcgg gaccgaccgt cttgcggaca
     2161 gcttcgagga tgcggaaggt gaagcgcagg cggttgtcga tgctgccgtt gtgctcgtcg
     2221 gtccgtcgat tggtggcggg tgaccagaag ctgtccatca gatggccgta gcattcgaac
     2281 tcgaacccat cgaggccggc tgccttcatg cgctcggcgg ccgccgcgta gtcggccgcg
     2341 atgcggtcga agtcccagtc ttccatttcc ttcggaaagg cgcgatgggc cggttcgcga
     2401 gcaggcgaag ccgacagaac cggaagccag tcgcccttgt tccagttggt gcggcggccg
     2461 agatgcgtca gctggatcat gacagccgca ccatgaccgt ggcattcatc cgccagcttc
     2521 ctgagccagg gcacgatctc gtccttgtag gccagaagat tgccgaaagc gggcggcgag
     2581 tcgcgcgaca cgacggccga gcccgccgtc atggtcaggg caatgccgcc cctggccttc
     2641 tccacatgat agagacggta gcggtcggtt ggcatgccat tttcggcata gttcggttca
     2701 tgcgacgtgg acatgagccg attcttcagg gtgagatgct tcagttgata gggctgg
//
LOCUS       DBNGVR010000355         2240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4060168, whole genome shotgun sequence.
ACCESSION   DBNGVR010000355 DBNGVR010000000
VERSION     DBNGVR010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4060168"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="AC16A0_07540"
     CDS             <1..361
                     /locus_tag="AC16A0_07540"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR3105224.1"
                     /translation="YNLVLIPIAGDSELRILKYDQLVHFYGFAVTAWMLYFILQKRAP
                     QLRRTLAVYVFPALASMGLGAVNEIVEFSAVMLVPHTNVGGYYNTALDLVFNALGAIF
                     AMLVCAAWDWVQARSPR"
     gene            complement(340..1860)
                     /locus_tag="AC16A0_07545"
     CDS             complement(340..1860)
                     /locus_tag="AC16A0_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3105225.1"
                     /translation="MTRRRERASRSASGNGQRPFRQPVYRYPPMAIISEDSLQAIHAT
                     ALTILEEIGMKVLSDRARALYAAGGADVEAGSDRVRFGRDMLGNWLARAPSRFTLHAR
                     DPGKTLQVGDGHVFFSSVGGPAYVCDLDGGRRQGSYAEQCDFLKVVQSLNILHQEGGG
                     PFEALDLDSRTRHLDLYYAQATLLDKNWQPQGLGSAVALDAIDVAAISLGRTRDDMRG
                     HCVFTTIINTNSPLQLDVPMAEGLIIMAEHGQCNVITPFTLAGAMSPVTLPGALAQQH
                     AEAMAGIVLAQIVSPGAPVMYGGFTSNVDMKSGAPAFGTPEYTQAAQISGQLARLLGV
                     PFRSSNVTAANDVDAQAAYESMMSLWGAITGGANLILHAAGWLNGGLTASCEKLIIDA
                     EMLQMMAAWCDPPRVTPETLALEAVRDVGPAGHFFGTPHTLARYETAFYQPMISNWDN
                     YDTWLERGRETAPVKANRVWKKMLASYERPPLDPAVDAALKEFVARRKRHYLGDRA"
     gene            complement(1857..>2240)
                     /locus_tag="AC16A0_07550"
     CDS             complement(1857..>2240)
                     /locus_tag="AC16A0_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR3105226.1"
                     /translation="SQPVVSAWTQMTDEQGFVQQRLLDLGVRLVVSHVLQAVAKGHAT
                     FAYALSASPVTMTFGSIVLVTGRLPVDDLYRELARSEAAPAVARIGDCFSPGPVADAV
                     YAGHRFAREFDSSAAVVLRRERPDP"
BASE COUNT          390 a          715 c          739 g          396 t
ORIGIN      
        1 ctacaacctc gttctcatcc cgattgccgg ggactccgaa ctcaggatcc tgaagtatga
       61 ccagctcgtg catttctacg gcttcgcagt gacggcgtgg atgctgtact tcatcctgca
      121 gaagcgcgca ccgcagctgc gccggaccct ggccgtatat gtcttcccgg ccctggccag
      181 catggggctt ggcgccgtca atgagatcgt cgagttctcc gccgtgatgc tggtgccgca
      241 taccaatgta ggcggctact acaacacggc gctcgacctc gtgttcaatg cgctgggagc
      301 catcttcgcc atgctggtct gcgcggcatg ggactgggtt caggcgcggt cgccgagata
      361 gtggcgcttg cggcgggcga cgaactcctt cagcgccgcg tcgacggccg gatcgagcgg
      421 tggcctttcg tagctggcga gcatcttttt ccagaccctg ttggctttca cgggcgccgt
      481 ctcgcggccg cgctccagcc aggtgtcata attgtcccag ttggagatca tcggctggta
      541 gaatgcggtc tcgtagcgcg ccagagtgtg cggcgttccg aagaaatgtc cggcaggacc
      601 gacatcgcgg acggcctcaa gggccagcgt ctccggtgtc acgcgcggcg gatcgcacca
      661 ggctgccatc atctgcagca tctcggcatc gatgatcaac ttttcgcaag atgccgtcag
      721 accgccgttg agccagcccg ccgcatgcag gatgagattg gcgccgccgg tgatcgcccc
      781 ccacagcgac atcatgctct cgtaggcggc ctgggcatcc acgtcgttgg cggctgtcac
      841 gttgctggag cgaaagggca cgccgagcag gcgcgccagc tggcccgaga tctgcgcggc
      901 ctgcgtgtat tccggggttc caaaggccgg tgcccccgac ttcatgtcaa cgtttgaggt
      961 aaatccgccg tacatcaccg gcgcgccggg actcacgatc tgcgccagca cgattccggc
     1021 catcgcctcg gcatgctgct gcgccagcgc gccgggaagc gtcaccgggc tcatggcacc
     1081 tgccagcgtg aaaggcgtga tgacattgca ttgcccatgt tcggccatga tgatcagccc
     1141 ctcggccatg ggcacgtcga gctgcagcgg cgaattggtg ttgatgattg tcgtgaagac
     1201 gcaatgaccg cgcatgtcat cgcgcgtgcg gccaagcgag atcgctgcga cgtcgatggc
     1261 atcgagcgcc acggcgctgc cgagcccctg tggctgccag ttcttgtcga ggagcgtggc
     1321 ctgcgcgtag tagaggtcga gatggcgcgt gcgcgagtcg aggtcaaggg cctcgaaggg
     1381 gccaccacct tcctggtgca ggatgttgag gctctggacc accttgagga aatcgcattg
     1441 ctcggcatag ctgccttgcc gtcggccacc gtcgagatca cagacatagg cagggccgcc
     1501 gaccgaggag aaaaacacgt gaccgtcgcc aacctgcaag gtctttcccg gatcgcgcgc
     1561 atggagcgta aagcgagacg gcgcccgcgc gagccagttt cccagcatgt cgcgaccgaa
     1621 gcgcacgcga tcggacccgg cctcgacatc cgcgcctccc gcggcataga gcgcacgggc
     1681 gcggtccgag agcaccttca taccgatttc ctcgaggatc gtcagcgccg tggcatggat
     1741 ggcctgcagc gaatcctccg agatgatggc catgggcgga tagcggtaga ccggctggcg
     1801 gaagggtctc tgcccgttgc cagaggcact gcggctcgca cgctcgcgcc gccgcgtcac
     1861 ggatcaggcc tttcgcggcg gagtacgacg gccgccgagc tgtcgaattc gcgggcaaat
     1921 cgatggccgg cgtagacggc gtcggcaaca ggtcccggtg agaagcagtc gccgatgcgc
     1981 gccaccgccg gggcggcttc gctccgggcg agctccctgt agagatcgtc aacaggcagg
     2041 cgcccggtca ccagcacgat gcttccgaag gtcatggtga cgggactggc tgagagcgca
     2101 tatgcgaagg tggcatggcc ctttgccaca gcctgaagga cgtgggacac gacgaggcgc
     2161 acaccgagat ccaacaaccg ctgctggacg aagccctgct cgtcggtcat ctgcgtccag
     2221 gccgagacga ccggctggct
//
LOCUS       DBNGVR010000356         1517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4452733, whole genome shotgun sequence.
ACCESSION   DBNGVR010000356 DBNGVR010000000
VERSION     DBNGVR010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4452733"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="AC16A0_07555"
     CDS             complement(<1..1168)
                     /locus_tag="AC16A0_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3105227.1"
                     /translation="MTATAEDRERPQISFWNDPKVRSILFQIILLLVLAFMAYEIVVN
                     TMSNLKSRNIASGFGFLSKTSGFDIIQSLIPYSSSSTYGRAILVGFYNTLLVSGLSIV
                     AATIIGFIMGVARLSKNWLIARIGTFYVEIFRNIPLLLWIFIFYSAVLQPLPGPREAL
                     NLFDMFFLSNRGFMMPKPIFGAGSMLGFAGLALGVILAIVIARWSKARQMATGQTFPV
                     MWTSLALIFGLPLLGFAFAGFPLHWEYPALKGFNFAGGIDVIPELIAMFLALSIYTGT
                     YVAEAVRAGIMAVSHGQTEASYALGLRPGKALRLVVIPQAMRVIIPPLASTYLSLTKN
                     SSLAVAIGYPDLVATGGIVLNQSGQAIEIVGIFMFVYLTLSLITAGLMNWFNARM"
     gene            complement(1290..>1517)
                     /locus_tag="AC16A0_07560"
     CDS             complement(1290..>1517)
                     /locus_tag="AC16A0_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3105228.1"
                     /translation="EIKRVLGVKNEDGSAAGFGTGIGLDEEWVVYIVKAVGNYGEIFE
                     RNVGANTPLKIARGVNALWKNGGLQYAPPIR"
BASE COUNT          337 a          440 c          470 g          270 t
ORIGIN      
        1 tcatccgggc gttgaaccag ttcatcagcc cggccgtgat caggctaagg gtaaggtaga
       61 cgaacatgaa gatgccaacg atctcaatgg cctgacccga ctggttcagc acgataccgc
      121 ctgtcgcgac gagatccgga tacccgatgg caactgccaa ggaagagttc ttggtgagac
      181 tcagataggt actggccagc ggtggaatga tcacccgcat ggcctggggg atgaccacga
      241 gccgaagcgc ctttcccggc cgcagaccca gcgcgtagga ggcctccgtc tggccgtgac
      301 ttaccgccat gatgccggcc cggacggctt cggcaacata ggtcccggta tagatggaca
      361 aggccaggaa cattgcgatc aattccggta tgacgtcgat gccgcctgcg aagttgaacc
      421 ccttgagcgc aggatactcc caatgcagcg ggaagccggc aaaggcaaat ccgagcagcg
      481 gcagtccgaa gatcagggcg aggctggtcc acatcaccgg aaaggtctgt ccggttgcca
      541 tctggcgcgc cttcgaccac cgggcaatca cgattgcgag tatgacgccc agcgcaagac
      601 ctgcgaagcc aagcatggag ccggcgccaa agatcggctt cggcatcatg aagccgcgat
      661 tggagaggaa gaacatgtcg aagaggttca gcgcctcgcg ggggccggga agcggctgca
      721 gaacggcgct gtaaaagatg aagatccaca gcagcagcgg aatgttgcgg aagatctcca
      781 catagaacgt gccgatacgg gcaatcagcc agttcttcga gagccgcgcg acgcccatga
      841 tgaagccgat gatggtggcc gcgacgatgc tcaggcccga caccagcagg gtattgtaga
      901 agccgaccag gatggcccgg ccgtaggtcg acgagctgga atacggaatc agcgactgga
      961 tgatgtcgaa gccggaggtc tttgacagaa agccgaaacc ggatgcgata ttccggctct
     1021 tgaggttgga catcgtgttg accacgatct cgtaggccat gaaggccagc acgagaagca
     1081 gtatgatctg aaacaagatc gagcggacct tgggatcgtt ccagaaagag atctgtggtc
     1141 tttcacggtc ctctgccgtg gctgtcatcc cctataccct tccacgaggc gcacggcctc
     1201 ctccccagtg gacactgccg gttttcccgg tcttggtctg acttgaaacg agagggagtc
     1261 cggccggtgg ccggaccccc gatgtcggat cagcggatcg gcggtgcgta ttgcaggccg
     1321 ccgttcttcc acagggcatt gacgccgcgc gcgatcttca gcggcgtgtt ggcgcctacg
     1381 ttgcgttcga agatctcgcc atagttaccc acggccttga cgatgtagac aacccattcc
     1441 tcatcgagac cgatgccggt accgaatccg gccgccgagc cgtcctcgtt cttgacgccg
     1501 agcacgcgct tgatttc
//
LOCUS       DBNGVR010000357         6127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4649754, whole genome shotgun sequence.
ACCESSION   DBNGVR010000357 DBNGVR010000000
VERSION     DBNGVR010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4649754"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16A0_07565"
     CDS             <1..352
                     /locus_tag="AC16A0_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3105229.1"
                     /translation="LKARGVDLIHCSSGGFDGAKPQVGALYQVPLSAAVRRGAEIPTA
                     AVGLITTAQQAESILAAGDADLIALARQALEDPNWALHAQHELDGGDAVYAGWPQQAG
                     YAIRNKDRALGGRT"
     gene            complement(357..1352)
                     /gene="speB"
                     /locus_tag="AC16A0_07570"
     CDS             complement(357..1352)
                     /gene="speB"
                     /locus_tag="AC16A0_07570"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3105230.1"
                     /translation="MSLEMKNMSSVPGDSAFRRENLKGTQWEPTYAGALSFMRRKYTR
                     DLAGVDIAVTGVPFDQAVSHRSGTRMGPEGIRKASAENAWGPFWPWNFDPFETLAVID
                     YGDCFFDWGRKEDFPVTLENHAAGIIGSGAEMITLGGDHYITYPLLKAHAKKHGPLSL
                     VHFDAHRDVEPDDGGRIDHGTMFGYAVREGLIDPKRSVQIGIRTVFTGERTYGFRIVY
                     ADEVHDSSAQQVADIVSKVVGTNKAYLTFDIDCLDPSVAPGTGTPIPGGLSYHQAASI
                     MRKLTGINFVAMDLVEVSPPYDHSEVTSGAAASLIMEYLCLRAWQRGARSVPIPE"
     gene            complement(1356..2444)
                     /locus_tag="AC16A0_07575"
     CDS             complement(1356..2444)
                     /locus_tag="AC16A0_07575"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105231.1"
                     /translation="MTEPIVSIRNVSKKFAGGVIAVDNISFDIDQNEFFALLGPSGCG
                     KTTLLRMISGLETPTSGQIMIGGQDMALTPPNKLPTNMVFQSYAVFPHMTVEQNVAYG
                     LKVTGVAPDEIKRRVAEGLEMVKLTHLAERRPDQMSGGQRQRVALARALVKRPKVLLL
                     DEPLSALDAKLRDDMRMELTRLQETVGITFIIVTHDQDEALSMASRIAVMEKGAVQQI
                     ATPVELYEHPKNRFVADFIGKVNLIDATVTGQKGKAITCDAKGLGKVELTSASQVGSA
                     IAIAVRPEKLKIGKTEPKSDKILKLQGKVRDVAYYGDTSHVVIEGKDGLDLSVNVQNE
                     SREGGSGVERGQKVWVHWAPEDSIVLTE"
     gene            complement(2448..3296)
                     /locus_tag="AC16A0_07580"
     CDS             complement(2448..3296)
                     /locus_tag="AC16A0_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105232.1"
                     /translation="MAAQLKPGQGPLDYGNRLWLKVLFAAIFFALYMPIITLVAFSFN
                     TDKRGIVWRGFTLENYTKAWNNVSLQEALINSLVIALLATILATIIGTLVALLLWRFR
                     FPMKGAYEGFMALPIVIPEICMGVALLMFFVNTGMMNAVVNMPWPLNLSNIVIAHVSF
                     CFPFVAVVVRSRLVGFNRQLEEASKDLGASEWQTFWSIIVPYMKPGLIAGALLAFTLS
                     LDDFVITFFTSGPETVTFPVKVYSLVRRGVSPEINAASTVLIVITVIATVIAMKLQAP
                     EKSGKG"
     gene            complement(3296..4168)
                     /locus_tag="AC16A0_07585"
     CDS             complement(3296..4168)
                     /locus_tag="AC16A0_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105233.1"
                     /translation="MESWKENRNVFWTFAAPGTFWLLAFFVIPLAFLFFMSFGEKAVI
                     DGQVSITEIDITGTLSNYLRAFDPVFLKIIWKSIWVSALATALCLATAYPIAFGICFA
                     PPKWKPLLLLLVILPFWINLLIRTYALIAVFRRQGYVNSVLGWVGLGPFDMLNNDFAV
                     ITGLVYVYMPFMVLPLYATIERLDRSFLEASLDLGASQFQTFMKVTVPLTMPGIISGI
                     ILVFIPCLGSFLTPDLLGGTDSQMIGNVIERQFKSANDWPFGAALSFLLMYATFGALA
                     LRWLFSRNSKGVDV"
     gene            complement(4243..>6127)
                     /locus_tag="AC16A0_07590"
     CDS             complement(4243..>6127)
                     /locus_tag="AC16A0_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3105234.1"
                     /translation="GYIHGAPAGRRLVWDTQHAGDLADHVEGLLPEDRPLAREVLSTH
                     RVLTVPKLAKLRSQIIHGDIHPYNTLMADDATITGLIDFGDLVHAPLVQDLSNAVSDF
                     LFPGRDHAATIYEMVRGYCRAAPLDEAETEVILDMIEVRLLMTALVESLKASMGISPQ
                     GYFGQFNSRAIPLIRELRAIGRDRLHATIRRAAAFPPAAAAPQSGDILERRRQVMGQK
                     PYVFYDPPLHMVRGDGIWLFDDTGRRFLDCYNNVPCVGHCHPYVTEAIARQARKLNTN
                     TRYLTDESINYAERLIELSHESLGAVVFVNSGSEANDLAWRMAKAWTGNRGGLAMEFA
                     YHGVSEAIDAFSPSNAPASWNAPHIRLLAPPDDYRGPYRRGEPGLAEAYAAMADAPIA
                     ALREAGFGLAAFMTDSAFMTIGILDVAPGYLQAVVDRVHTAGGLFIADEVQSGYGRMG
                     THFWGHRHHGVVPDFITIGKPAGNGYPVGAVITRHEILENFLRNGPFFSTFGGGNVAC
                     AAGIAVLDVVRDEGLVENARVTGACFREGLRSLMQRHGLIGDVRGVGLATGVELVRDR
                     DSREPADRETSRLVNLLRDEGVLMGSEGRLGNIVKIRPPLVFSRENAEFAVAAIDRAL
                     ARL"
BASE COUNT         1169 a         1886 c         2006 g         1066 t
ORIGIN      
        1 actgaaggcg cgcggcgtcg atctcatcca ctgttcgtcg ggcggcttcg atggcgccaa
       61 gccgcaggtg ggcgcgctct atcaggttcc cttgtcggcc gccgtgcggc gtggtgcgga
      121 gattcccacc gccgccgtcg ggctcatcac aaccgcgcag caggccgagt cgatcctcgc
      181 tgcaggcgac gccgatctca tcgcactggc gcggcaggcg ctggaagatc ctaattgggc
      241 tcttcatgcc cagcacgagc tggatggcgg cgatgccgtc tatgccggat ggccgcagca
      301 ggcgggctac gcaattcgca acaaggaccg cgcgctgggc ggccggacgt aaggcctcat
      361 tccgggatcg gcaccgaccg cgcgccgcgc tgccaggcgc gcaggcacag atattccatg
      421 atcagcgaag ccgcggcacc agaggtgacc tcggaatgat catagggcgg cgacacttcg
      481 acgagatcca tggcgacgaa gttgatgccg gtcagcttgc gcatgatcga ggcggcctgg
      541 tgataactga ggccgcccgg aatgggcgta cccgtgccgg gcgcgaccga gggatcgagg
      601 cagtcgatgt cgaaggtcag ataggccttg ttggtgccca ccaccttgct gacgatatcg
      661 gccacctgct gggccgagct gtcatgcact tcgtcggcat agacgatacg gaagccatag
      721 gtgcgctcgc ctgtgaagac cgtgcggatg ccgatctgaa cggagcgctt cggatcgatg
      781 aggccttcgc ggacggcgta accgaacatg gtgccgtgat cgatgcggcc gccgtcatcg
      841 ggctcgacat cgcggtgcgc gtcgaaatgg acgagcgaca gggggccatg cttcttcgca
      901 tgggccttga gcagcggata ggtgatgtag tggtcgccgc ccagcgtgat catctcggcg
      961 ccggagccga tgattccggc tgcgtggttc tcgagcgtga ccggaaaatc ctccttgcgg
     1021 ccccagtcga agaagcagtc gccatagtcg atgaccgcga gcgtctcgaa ggggtcgaag
     1081 ttccagggcc agaacgggcc ccaggcattc tcggcagagg ctttgcggat gccctccggc
     1141 cccatgcgcg tgccggagcg gtgcgatacc gcctggtcga agggcacacc ggtgacggca
     1201 atatccacgc cggcaaggtc gcgcgtatac ttgcggcgca tgaaggagag cgcaccggca
     1261 taggtcggct cccactgcgt tcccttcaga ttctcgcgcc ggaaggcgct gtcccccgga
     1321 acggaggaca tgttcttcat ctcgagtgac atccgctact ccgtaaggac gatggaatct
     1381 tccggggccc agtgcaccca gaccttctgg ccgcgttcaa caccggagcc gccttcgcgc
     1441 gattcattct ggacattgac cgagaggtcg agcccgtcct tgccttcgat caccacgtgg
     1501 cttgtgtcgc cgtaataggc aacatcgcgc accttgccct gcagcttcag gatcttgtcg
     1561 gacttgggtt cggtcttgcc gatcttcagc ttctcgggcc gcaccgcaat ggcgatcgcg
     1621 ctgccaacct gcgacgcgct cgtcagttcc accttgccca gtccctttgc atcgcaggtg
     1681 atcgccttgc ccttctggcc ggtgacggtc gcatcgatga gattcacctt gccgatgaag
     1741 tccgccacaa accgattctt cggatgctcg tagagttcga ccggcgtggc gatctgctgc
     1801 acggcgccct tctccatgac cgcgatgcgg ctggccatgg agagcgcttc gtcctggtca
     1861 tgggtaacga tgatgaaggt gatgccgacc gtctcctgca accgggtcag ttccatccgc
     1921 atgtcgtcgc gcagcttggc atcgagcgcc gaaagcggtt cgtcgagcag cagaaccttg
     1981 gggcgcttca cgagcgcgcg ggcaagggcc acgcgctggc gctgcccgcc cgacatctgg
     2041 tcgggccggc gttccgcaag gtgggtgagc ttcaccatct cgaggccttc ggccacccgg
     2101 cgcttgatct cgtcgggcgc aaccccggtg accttcaggc catacgcgac gttctgctcc
     2161 actgtcatgt gcggaaacac cgcataggac tgaaacacca tgttggtggg cagcttgttg
     2221 ggcggcgtca gcgccatgtc ctggccgcca atcatgatct gccccgaagt tggcgtctcg
     2281 agaccggaga tcatccggag cagcgtggtc ttgccgcagc cggacggccc cagcagcgcg
     2341 aagaactcgt tctggtcgat gtcgaaggag atgttgtcga cggcgatgac gccgccggca
     2401 aacttcttgg acacattgcg gatggagacg atgggttcgg tcatggttca gcccttaccc
     2461 gacttttcgg gagcctggag tttcattgcg atgacggtgg cgataacggt gatcacgatc
     2521 agcaccgtgg acgctgcgtt gatctccggg gaaacgccgc ggcgcaccag cgagtaaacc
     2581 ttcaccggga aggtgacggt ttccggcccg gacgtgaaga aggtgatgac gaagtcatcc
     2641 agcgacagtg tgaaggcgag caaggcaccg gcaatcaggc cgggcttcat gtagggcacg
     2701 atgatgctcc agaaggtctg ccactcgctg gcgccaagat ccttcgaggc ttcctccagt
     2761 tgccggttga agccgacaag gcgcgatcgc accaccaccg cgacgaaggg gaaacagaac
     2821 gagacatggg cgatcacgat gttcgacaga ttgagcggcc atggcatgtt gaccaccgca
     2881 ttcatcatgc ccgtgttgac gaagaacatc agcaaggcca cgcccatgca gatttccggg
     2941 atgacgatgg gcagcgccat gaagccctcg taggccccct tcatcggaaa acggaagcgc
     3001 cagagcagca gggcgacgag cgtgccgatg atggttgcga gtatggtggc cagcaaggcg
     3061 atgaccagcg agttgatcag cgcctcctgc agcgagacgt tgttccacgc cttggtgtag
     3121 ttctccagcg tgaagccgcg ccagacgatg ccgcgcttgt cggtgttgaa ggaaaaggcc
     3181 accagcgtga tgatgggcat gtagagcgcg aagaagatcg cggcgaacag caccttgagc
     3241 cagaggcggt tgccgtagtc gagaggcccc tgccccggtt tcagctgcgc ggccatcaga
     3301 catccactcc cttgctgttc ctggagaaga gccagcgcag cgccagggcg ccgaaggtgg
     3361 cgtacatgag caggaaggac aaggcggcgc cgaagggcca gtcattggcg gatttgaact
     3421 gccgctcgat cacattaccg atcatctgcg agtccgtacc gccgagcagg tccggcgtca
     3481 ggaaggatcc gaggcacgga atgaagacaa ggatgatgcc ggagataatg cccggcatgg
     3541 taagcggaac cgtgaccttc atgaaggtct ggaactgcga ggcgccgagg tcgaggctgg
     3601 cctccaggaa cgagcgatcg agacgctcga tggtggcgta gagcggaagc accatgaagg
     3661 gcatgtagac atagacaagg ccggtgatga cggcgaaatc attgttcagc atgtcgaagg
     3721 ggccgaggcc gacccatccc agcacgctgt tgacatagcc ctgccggcga aacaccgcga
     3781 tcagcgcata ggtgcgtatc agcaggttga tccagaaggg aagaatgacg agcagcagga
     3841 gcagcggctt ccacttgggc ggtgcgaagc agatgccgaa ggcgatggga taggcggttg
     3901 caaggcagag cgccgtcgcc agggcgctga cccagatcga cttccagatg atcttgagga
     3961 agaccgggtc gaaggcgcgg agatagttgg acagcgtgcc ggtgatgtcg atctcggtga
     4021 tcgaaacctg cccgtcgatc accgccttct cgccgaagga cataaagaac aggaacgcga
     4081 ggggaatgac gaaaaaggcc agaagccaga acgtgcctgg tgcagcaaac gtccagaaga
     4141 cgtttcggtt ttccttccag ctctccaaga tccgccctct ccccgtttgg ttttttcttg
     4201 aacctagccc aggccgaggg gcctcggcaa cccgcctgtg cctcagagcc gggcgagcgc
     4261 ccggtcgatc gccgcaacgg cgaattcggc attctcccgc gagaacacca gcggcggcct
     4321 gatcttcacg atgttcccga gccttccctc gctccccatc agcacgccct cgtcacgcag
     4381 caggttcacc agacgcgagg tttcccggtc ggccggctcg cggctgtccc tgtcgcgcac
     4441 gagttccacg ccggtggcga ggccgacgcc ccgtacatcg ccgatgaggc catggcgctg
     4501 catcaggctg cggagccctt cgcggaaaca ggcgccggtg acgcgggcat tctcaaccag
     4561 cccttcgtcg cgcaccacgt cgagcaccgc gatccccgcc gcgcaggcga cattgccccc
     4621 gccgaaggtg gagaagaacg gtccgttccg gaggaagttt tccaggatct cgtggcgcgt
     4681 gatcaccgca ccgaccgggt atccgttgcc cgccggcttg ccgatggtga tgaagtccgg
     4741 aaccacgcca tgatgccggt ggccccagaa atgggtgccc atgcggccat atcccgactg
     4801 cacctcgtcg gcgatgaaca ggccgcccgc cgtatggacc cggtcgacaa cagcctgcag
     4861 ataaccgggc gcgacatcga ggatgccgat cgtcatgaag gcggagtcgg tcatgaaggc
     4921 ggccaggccg aagcctgcct cccgcagcgc ggcaatcggt gcatcggcca tggccgcata
     4981 ggcctcggcc agtcccggct ccccccgccg gtaagggccg cgatagtcat ccggcggcgc
     5041 aagcagccgg atatgcggcg cgttccagct tgcaggcgca tttgagggcg agaaggcatc
     5101 gatggcttcc gagacgccgt gataggcgaa ttccatcgca aggccgccac gattgcccgt
     5161 ccaggccttt gccatgcgcc aggccaggtc attggcctcg ctgccggagt tcacgaagac
     5221 aacggcacca aggctctcat gcgagagttc gatcaaccgt tcggcatagt tgatcgattc
     5281 atccgtcaga tagcgcgtgt tggtgttgag cttccgcgcc tgccgggcaa tggcctcggt
     5341 cacataggga tggcagtgcc ccacgcatgg cacattgttg taacagtcca ggaagcgccg
     5401 cccggtgtca tcgaagagcc agatgccgtc cccgcgcacc atgtgaagcg gcggatcgta
     5461 gaagacgtaa ggcttctgcc ccatgacctg gcggcggcgt tcgaggatat ctccggattg
     5521 cggcgctgct gctgccgggg ggaaggcggc cgcccggcgg atcgtcgcat gaaggcggtc
     5581 gcggccgatg gccctcagct cgcggatcag cggaatggcc cggctgttga actgtccgaa
     5641 atagccctgc ggagaaatgc ccatgctcgc cttgaggctc tcgaccagcg ccgtcatcag
     5701 caggcgcacc tcgatcatgt cgaggatcac ctcggtttcc gcctcgtcca gcggcgcggc
     5761 ccggcaatac ccccgcacca tctcgtagat ggtggcggcg tgatcgcggc cgggaaagag
     5821 gaagtcggag acggcgttgg acagatcctg aaccagcggc gcatgcacga ggtcgccgaa
     5881 gtcgatgagg ccggtgatcg ttgcatcgtc ggccatgaga gtgttgtagg gatgaatgtc
     5941 accgtggatg atctggctgc gcagcttcgc aagcttcggc acggtcagca cccggtgcgt
     6001 ggacaggacc tcgcgcgcca gcggccggtc ttccggcagg agaccctcga catggtccgc
     6061 caggtcgccg gcatgctgcg tgtcccagac gaggcgcctt ccggccggtg cgccgtgaat
     6121 gtagccg
//
LOCUS       DBNGVR010000358         1994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3864654, whole genome shotgun sequence.
ACCESSION   DBNGVR010000358 DBNGVR010000000
VERSION     DBNGVR010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3864654"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="AC16A0_07595"
     CDS             <1..1323
                     /locus_tag="AC16A0_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3105235.1"
                     /translation="DAIVPRDHLVREIAAVLDLSWVYKELAPHYSTMGRPSIDPVLMI
                     RMLIIGYVFAIRSERALCREVQVNLAYRWFCGLSIEDNLPDHSAFSRARNERFHDSDV
                     FRQVFERVVGACIAAGLVGGAGFAVDASLIAADANKHRSIPGGDWSRDIDPERSSRAV
                     KEYLATLDDPAYGTASEVTPKFISPSDPAAQWTGALRNAAFFAYADNYLIDVKFGVIM
                     DVQASRAIRQAEVGASRAMIERTETCFGIKPQWLAADTAYGSASNLDWLVNQKGIAPH
                     VPVIDKSKRDDGTFSRDAFIYDRDRDTYTCPAGKTLKTSGTLVNGGATLLYRASTSDC
                     GPCLFKSQCCPVSPQRKVPRDIHEDARDVARGLARTEEFAKSRRDRKKVEMLFAHLKR
                     ILRLGRLRLRGPRGAQFEFTLAAIAQNLRRLGRLIARPPPGMTVACIR"
     gene            complement(1610..>1994)
                     /locus_tag="AC16A0_07600"
     CDS             complement(1610..>1994)
                     /locus_tag="AC16A0_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR3105236.1"
                     /translation="DTETGSYIRNAHAPCLTREEMIYYLESFLGPRGGANWSDPYAVP
                     NLSTNLRALPPAFITVAAHDPLCDDGLIFRDKLKAEGVAVALREEPVLAHSYMRARHV
                     SAPAMAGFKAIAEAVRSLAHEGMLP"
BASE COUNT          384 a          616 c          603 g          391 t
ORIGIN      
        1 gatgcaatcg tgccgagaga tcatctggtt cgggagattg cagctgttct cgacctgagc
       61 tgggtctaca aggagctggc accccactat tcgacgatgg gtcggccatc gattgacccg
      121 gtgctgatga tccggatgct catcattggc tacgtctttg cgattcgctc agagcgggcc
      181 ttgtgccgtg aagtgcaggt caacctggcc taccgctggt tctgcggcct gagcattgag
      241 gacaatctgc cggatcactc ggcgttctcc cgagcccgca acgagcggtt ccatgacagc
      301 gatgtgttca ggcaggtttt tgaacgtgtt gtaggagcct gtattgcggc aggcctcgtg
      361 ggcggcgccg gctttgccgt ggacgccagt ctgatcgccg ctgacgccaa caagcaccgc
      421 tcgatcccgg gtggtgactg gagcagagac attgacccag agcgatcaag ccgggccgtc
      481 aaggagtatc tggccactct tgatgatcca gcatatggca cagcaagcga ggtgacgccg
      541 aagttcatct cgccgtccga tccggctgct caatggaccg gcgccctgcg caatgctgca
      601 ttcttcgcct atgccgacaa ctatctcatc gacgtgaagt ttggtgtcat catggacgtt
      661 caggcctcac gtgccattcg ccaggctgaa gtcggtgcgt cgcgagccat gattgaacgg
      721 accgagacgt gcttcggcat caaacctcaa tggcttgctg ccgatactgc atacggatcg
      781 gcctccaacc tcgactggct ggtgaaccag aagggaatcg cgccccatgt tccggtcatc
      841 gacaagtcca agcgtgatga tggcactttc tcgcgtgacg cgttcatcta tgacagggac
      901 cgcgacacat atacctgtcc ggccggcaaa acccttaaaa ccagcggaac gctggtcaac
      961 ggcggggcaa cattactcta tcgggcgagc acctccgact gcggcccctg cctgttcaag
     1021 tcccaatgtt gtcccgtatc gccacaacgc aaggtgccgc gcgacataca tgaagatgcc
     1081 cgtgatgtgg ctcgtggcct cgccaggaca gaagagtttg ccaagtcacg tcgagatcgc
     1141 aagaaggtcg agatgctgtt cgcccacctg aaacgcatcc ttcggctcgg gcgactgcga
     1201 ctccggggtc cacgcggcgc gcagttcgag ttcactcttg ctgccattgc ccagaacctg
     1261 cggcgtctcg gcaggctaat cgcccgaccg ccgccgggca tgacagtggc gtgcataaga
     1321 tgaggtcccg gtcgtgtgct gtgcttccca agcatgatcc tgacaccagc acagcttcag
     1381 ccggagctgg caatagaagg attgcctgcc gcttccttaa gaacgcccga cataagcact
     1441 ttccgtgctc acaacgactt ctacaacagt atcggccctt ctgcgacatc gcagcgatcg
     1501 ccgggactga ccgcttccgc accggaagca gacatgcggg cagaaccaac cctgtccatt
     1561 gatgagccca cgccgtggac tgggctccag cacgggaacc ccctttctgt cagggcagca
     1621 tgccctcgtg cgccaggctg cgcaccgctt cggcgatggc cttgaatccc gccatggcgg
     1681 gcgcgctcac atggcgcgcg cgcatgtagg aatgcgccag caccggctcc tcgcgcagcg
     1741 caacggcaac cccttcggcc ttcagcttgt cgcggaagat cagtccgtcg tcgcagagcg
     1801 ggtcatgggc cgccaccgtg atgaaggccg gcggcagcgc gcggagattg gttgaaagat
     1861 tgggcaccgc ataggggtcg ctccagttcg cgccgccgcg ggggccgagg aagctttcga
     1921 gatagtagat catttcctcg cgcgtgaggc agggcgcatg agcgttgcgg atgtaggagc
     1981 ccgtctcggt gtcg
//
LOCUS       DBNGVR010000359         2176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2096380, whole genome shotgun sequence.
ACCESSION   DBNGVR010000359 DBNGVR010000000
VERSION     DBNGVR010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2096380"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="AC16A0_07605"
     CDS             <1..1923
                     /locus_tag="AC16A0_07605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3105237.1"
                     /translation="AFGLAALFGYIWLTLSQNGVLTIPEREPGLMILAADGTMLAEQG
                     AFRGDEARLADLPDYVPNALIAIEDRRFRSHYGVDPIGLGRAIFTNLASGRLVQGGST
                     LTQQLAKNLFLSPARTLERKLQEAVLAVWLETQYSKDEILQLYLNRVYYGAGATGIEK
                     AAQTYYRKSASELSLTEAATLAGILPAPSNYNPLKNPEAAARRARLVLQSMEEEGFIS
                     PAEAQLAINAPAEVRASDYIPATQFVADWVREQLPELVGSYDQSIVIETTIDPSIQAS
                     AELALRKRLNEEGRKVKASEGALVMLDGLGAVKAMVGGKSYRKSQFNRATKAKRQPGS
                     AFKPFLYLAAIEQGYGPDTVEVDEPVRIGNWQPENYRQNYLGPVRLETAFALSLNTIA
                     AKLVMLVGPERVAATARRLGITSPLGSDASIALGTSEVTLLELTAAFVPFANNGYPVI
                     PHVVTRISTRDGQLLYERHGDGYTQAITDFDLGALNRMMRAVVTEGTGKRAQFGAYDI
                     GGKTGTSQDYRDAWFIGYTADYTAGVWVGNDDNSPTRNVTGGALPAEIWRDVMQQAHR
                     GLSVRPLPGQTISRDPDLTTISRIDVPSQDIAGDAQPETPKRKGFFEKLGSLFSGGSD
                     DSRPKPHWQTRNKENR"
     gene            complement(1968..>2176)
                     /locus_tag="AC16A0_07610"
     CDS             complement(1968..>2176)
                     /locus_tag="AC16A0_07610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3105238.1"
                     /translation="GGELSYSWRLILTPPHVLDYVAAHEVAHLKHMNHGPRFWRLVLT
                     HCPDAAAAKRWLKTHGQSVHRIRM"
BASE COUNT          393 a          725 c          701 g          357 t
ORIGIN      
        1 gccttcggcc tcgcggcgct cttcggctat atctggctga cgctcagcca gaatggcgtg
       61 ctgaccatcc cggagcgcga gcccgggctg atgatcctgg ccgccgacgg caccatgctg
      121 gccgagcagg gcgctttccg cggcgacgag gcccgcctcg ccgatcttcc cgactatgtg
      181 cccaatgcgc tgatcgccat cgaggatcgt cgtttccgca gccattacgg tgtcgatccc
      241 atcggccttg gccgcgccat cttcaccaat ctggcctcag gccgcctggt gcagggcggc
      301 tcgaccctca cccagcagct ggccaagaac ctgttcctgt cgccggcccg caccctggag
      361 cgcaagctgc aggaagcggt cctcgccgtc tggctggaaa cgcagtacag caaggacgag
      421 atcctgcagc tctatctgaa ccgggtctat tacggcgcgg gcgcaacggg catcgagaag
      481 gcggcccaga cctattaccg caagtctgcg tccgagctca gcctcaccga ggccgccacg
      541 ctggcaggga ttctccccgc gccttcgaac tacaatccgc tgaagaatcc ggaggcggcg
      601 gccaggcgcg ccaggctggt gctgcagagc atggaggagg agggcttcat ctcgccggcg
      661 gaggcccagc tcgcgatcaa tgcgcctgcc gaagtgcggg cctccgacta cattcccgcc
      721 acgcagttcg tggccgactg ggtgcgcgaa cagctgcccg aacttgtcgg gagctatgat
      781 cagtccatcg tcatcgagac caccatcgat ccgtcgatcc aggcttccgc cgaactcgcg
      841 ctgcgcaagc ggctcaacga ggaaggccgc aaggtgaagg cctcggaggg cgcgttggtg
      901 atgctggatg gtctcggggc ggtcaaggcc atggtcggcg gcaagtccta tcgcaagagc
      961 cagttcaacc gcgcgaccaa ggcaaagcgc cagccgggat cggccttcaa gcccttcctc
     1021 tatctggcgg ccatcgaaca gggctacggg ccggacacgg tcgaggtcga cgagccggtg
     1081 cgtatcggca actggcagcc ggaaaactac cgtcagaact atctcgggcc ggtcaggctc
     1141 gagacggcct tcgcgctctc gctcaacacc attgccgcaa agctggtcat gctggtggga
     1201 ccggagcggg ttgccgcaac ggcgcgccgg cttggcatca cgtcgcccct ggggagcgat
     1261 gcctcgatcg cacttggcac ctcggaagtg accctgcttg aactcacggc cgccttcgtg
     1321 cccttcgcga acaatggtta tccggtgatc ccccatgtgg tgacgcggat ttcgacgcgc
     1381 gacggccagc tgctctacga gcgccatggc gacggctata cccaggcgat cacggacttc
     1441 gatctcggcg ccctcaaccg catgatgcgc gccgtggtca cggagggcac gggaaagcgc
     1501 gcgcagttcg gcgcatacga catcggcgga aagaccggca ccagccagga ttaccgcgat
     1561 gcctggttca tcggctacac ggccgactac acggccggcg tctgggtcgg caatgacgac
     1621 aactcgccga cccgaaatgt cactggcggc gcgttgcccg ccgagatctg gcgcgatgtc
     1681 atgcagcagg cccatcgcgg cctctcggta cggccgctgc ccggccaaac gatcagccgc
     1741 gatccggacc ttaccacgat ttcgcgcatc gacgtgccct cgcaggatat agcgggtgac
     1801 gcgcagccgg agacgccgaa gcgcaagggg ttcttcgaga agctgggcag cctcttctcc
     1861 ggcggctcgg acgattcgcg tcccaagccc cactggcaga cccgcaacaa ggaaaaccgc
     1921 tgaagaaatc tgccgctccc gtcttcggcg ggagcgcagt gtttcggtca catacggatg
     1981 cgatgcacgc tttgcccgtg tgtcttgagc cagcgcttcg cggctgcggc atcggggcaa
     2041 tgcgtgagaa cgaggcgcca gaaccgcggg ccatggttca tgtgcttgag atgcgcgact
     2101 tcatgagcgg ccacatagtc gagcacatgc gggggcgtga ggatcaggcg ccaggagtag
     2161 gacagttcgc cgcccg
//
LOCUS       DBNGVR010000360         2624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4650229, whole genome shotgun sequence.
ACCESSION   DBNGVR010000360 DBNGVR010000000
VERSION     DBNGVR010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4650229"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /gene="mobA"
                     /locus_tag="AC16A0_07615"
     CDS             complement(<1..586)
                     /gene="mobA"
                     /locus_tag="AC16A0_07615"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HIR3105239.1"
                     /translation="MRIAGAVIAGGRSSRMGQEKAFAILGGQTLLAHVIARIASQTDE
                     VFINANGDPARFDGFGRAVIPDLSSGIASPLAGLQTCLQFAAAGHFDAVLTVPSDSPF
                     VPRDLVRRLAEGSPRAAIARSGGQDHFLTGLWSVGLRPELDRAMAQEGLVRVKDWVRR
                     CHAVSVAWPVGPHDPFFNVNTPEDLADARRIAAGF"
     gene            complement(583..1167)
                     /locus_tag="AC16A0_07620"
     CDS             complement(583..1167)
                     /locus_tag="AC16A0_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3105240.1"
                     /translation="MSELLSKAPFTGRAARLLSSPDRKSGLEKAECEALVLRFSGIEG
                     DCHGGLIRKSDSRMVKQYKRGTMVRNARQLSILSTEELADIARSMAIPDVRAEWVGAN
                     LVTTDIPDLTLLPPSTRLQFPSGAMIVVDIENLPCRYPAAIIEKQHPEQAMGFVKAAK
                     HKRGVVGWVEAEGDIRVGDAITVWLPPQRLYAHA"
     gene            1208..1444
                     /locus_tag="AC16A0_07625"
     CDS             1208..1444
                     /locus_tag="AC16A0_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3105241.1"
                     /translation="MRENHSDSVIDLSGLLCPLPVLKARKRLESLPSGHVLKVIATDP
                     MSAIDMPHFCNEQGHTLLDQVREADALIFRIRRR"
     gene            1441..2319
                     /locus_tag="AC16A0_07630"
     CDS             1441..2319
                     /locus_tag="AC16A0_07630"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF039614.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR3105242.1"
                     /translation="MSFDFGSFNNWGQLKTVAIRDVDTAFASDARIDAEWKDLNYHSR
                     PDLANARKEYEAVEEILSAAGAEVIKLPAGDGLTLDSIYTHDALIVTPRGLVKPRMGK
                     PQRRRESEVNGAALAAMGFPIAGEITGEGKVEGGDLIWIDRYTLLAGIGYRTNLEGVR
                     QLGELAGEDVEILWFDMPHYKGRSDVFHLMSTLSPLDHDLAVAYPPLMAARQIEFLES
                     RGIRFVEVPPEEFDSMGCNVLALGPRHAMMVEGNPETERRLRVAGVKVEVIKGYDICR
                     KGEGGPTCMTRPLVRG"
     gene            complement(2339..>2624)
                     /locus_tag="AC16A0_07635"
     CDS             complement(2339..>2624)
                     /locus_tag="AC16A0_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HIR3105243.1"
                     /translation="DHKIPHSFSDVKDNPITKEQVAAWSKALGGWEKMINKAGYTWRG
                     LPPAETANLTEAKAVALAVKNPSLIRRPLIEHKDGSVTVGFSKKIQELLG"
BASE COUNT          469 a          838 c          845 g          472 t
ORIGIN      
        1 ggaaccctgc cgcgatccgc cgggcatccg caaggtcttc cggcgtgttg acgttgaaga
       61 agggatcgtg cggcccgacc ggccacgcga cggacaccgc gtggcagcgc cgtacccaat
      121 ccttgacgcg caccagaccc tcctgcgcca tcgcacggtc aagctcgggg cgtagcccga
      181 cggaccacag gccggtcagg aaatggtcct gaccgccgga ccgcgcgatg gctgcgcgcg
      241 ggctgccttc ggcaagtcgc cgtaccagat cacgcgggac gaagggcgaa tcggagggca
      301 ccgtgagcac cgcatcgaaa tgtcccgccg ctgcaaactg caggcaggtc tgcagccctg
      361 ccagcggcga tgcgatgccg ctgctcaggt cgggtatgac ggcacgtcca aatccatcga
      421 agcgtgccgg atcgccattg gcgttgatga agacctcgtc cgtctgggag gcgatccggg
      481 cgatcacatg cgccagcaag gtctggccgc ccagaatcgc aaaggccttt tcctgaccca
      541 tgcgcgatga ccgcccgccg gcgatgacgg cacctgcaat cctcatgcgt gggcatagag
      601 ccgctgcggc ggcagccaga ccgtgatggc atcgccgacc cggatgtcgc cctcggcctc
      661 gacccagccc accaccccgc gcttgtgctt ggccgccttg acgaagccca tcgcctgctc
      721 gggatgctgc ttttcgatga tggcggccgg atagcggcag ggcaggtttt cgatgtcgac
      781 gacgatcatg gcgccgctcg ggaactgcag ccgcgtcgac ggcggcagga gcgtgagatc
      841 gggaatgtcc gtggtcacca gattggcgcc gacccattcc gccctgacgt ccgggatagc
      901 catgctgcgg gcgatatcgg ccagctcctc cgtcgagagg atggacagct ggcgcgcatt
      961 ccttaccatg gttccgcgct tgtactgctt caccatgcgg ctgtcggatt tccggatgag
     1021 tccgccgtga cagtcgccct cgatgccgga gaagcgcagg accagcgcct cgcattcggc
     1081 cttttccagg ccggactttc ggtcggggct ggacagaagc cgtgcggcac ggccagtgaa
     1141 gggggctttg gacagaagct cggacaatgg tgtaactccc gcaatgaggg atcatgttag
     1201 ccagcccatg cgtgaaaatc attccgattc cgtgatcgat ctgtccggac tgctctgccc
     1261 gctgccggtg ctgaaggcgc gcaagcggct ggagtcgctg ccgtcgggtc atgtgctgaa
     1321 ggtcattgcc acggacccca tgtcggccat agacatgcct cacttttgca acgaacaggg
     1381 ccatacgctg ctcgatcagg tccgggaagc ggacgccctc atattcagga tcaggcgcag
     1441 atgagtttcg atttcggcag tttcaacaac tggggacagc tgaagacagt ggcgatccgc
     1501 gatgtcgaca ccgccttcgc cagcgatgcc aggatcgatg cggagtggaa ggacctgaac
     1561 tatcattcca ggccagacct cgccaatgca cgcaaggagt acgaggccgt cgaggagatc
     1621 ctctcagccg caggagccga agtcatcaag cttcccgcag gcgacggtct gacgctggat
     1681 tcgatctata cccacgatgc cctcatcgtc acgccgcgcg gcctggtcaa gccccgcatg
     1741 ggcaagccgc agcggcgccg ggagtcggag gtgaacggcg ctgcgctggc ggccatgggc
     1801 tttcccatcg ccggcgaaat caccggcgag ggcaaggtgg aaggcggcga cctcatctgg
     1861 atcgatcgct atacgctcct ggccggcatc ggctatcgca ccaatctcga gggcgtgcgg
     1921 cagctgggcg agctggccgg cgaggatgtc gaaatcctgt ggttcgacat gccgcattac
     1981 aagggccgtt cggatgtctt tcacttgatg agcacactgt cgccgctcga ccatgatctt
     2041 gccgtggcct atccgccgct gatggcggcc cgccagatcg agttcctcga atcgcgcggc
     2101 atccgcttcg ttgaagtgcc ccccgaggaa ttcgattcga tgggctgcaa cgtgctggcg
     2161 ctggggccgc gtcacgccat gatggtggag ggcaatccgg aaaccgaacg ccgcctccgg
     2221 gttgccggcg tcaaggtcga ggtcatcaag ggttacgaca tctgccgcaa gggcgaaggc
     2281 ggcccgacct gcatgacgag gcccctggtc cgcggctagg cgcgaacctc ccccgtcgtc
     2341 atccaagaag ttcctgaatc tttttcgaga agcccaccgt cacgctgccg tccttgtgtt
     2401 cgatcagcgg ccggcggatc agcgacgggt tcttgacagc cagtgcaacg gctttggcct
     2461 cggtgaggtt ggcggtctct gccggcggaa ggccgcgcca cgtatagccg gccttgttga
     2521 tcatcttctc ccagccgccc agcgccttcg accaggccgc gacctgttcc ttggtgatgg
     2581 gattgtcctt cacatcggaa aaactgtggg ggatcttgtg atcc
//
LOCUS       DBNGVR010000361         1612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1901605, whole genome shotgun sequence.
ACCESSION   DBNGVR010000361 DBNGVR010000000
VERSION     DBNGVR010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1901605"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="AC16A0_07640"
     CDS             <1..938
                     /locus_tag="AC16A0_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3105244.1"
                     /translation="SGAHAIGVHVTPRGLEAALVDLAGSIIAREHRALERPGPDQTMT
                     EIGRLAKQFGGKLKGKRMLGIGMAMPGPFDVESMSFVGPTTLEGWRGFPVLERLNRVS
                     GLPAFIEVDHAAAALGERLHGAGKAFRDFYFLYFGVGLGGCMVHEGLPWRGSHRNAGE
                     IGHLPLVPNGEPCPCGNRGCLERYLSLEAYGRRATAIGREAWISEAAPLFRNAVAAIE
                     NLFDPETIIVGGLADSGLLDDLIAAAQPLPNSVSQRADREVPRLMRADSGQDGVLRGA
                     AALAIAGVLSPRFGLLFAEGERTERDPIIAGRQVA"
     gene            935..>1612
                     /locus_tag="AC16A0_07645"
     CDS             935..>1612
                     /locus_tag="AC16A0_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3105245.1"
                     /translation="MSSDTLLRLKDITRNFGAIEALRGISFEVQRGEVLALLGDNGAG
                     KSTLVKIISGGLKPTSGSLYFDGRERNFASPADAKAEGIETVYQDLSLCTNVDVVANF
                     FMGREVVKRYFGIPVLQEAEMEAAVARAMSNAGTRIPSLRTNVEYLSGGQRQAIELNR
                     FVHWGGKLVLLDEPFAALGVEQTRRGLEMIRRIADQGIGVIIITHIMAQAFQVADRIV
                     VIRQGLVA"
BASE COUNT          273 a          534 c          536 g          269 t
ORIGIN      
        1 cctccggcgc ccatgccatc ggcgttcatg tgacgccgcg cggactggag gcggcgctgg
       61 tcgatctcgc cggctccatt atcgcgcgcg aacaccgggc ccttgaaagg cccgggccgg
      121 accagaccat gaccgagatc ggacggctgg caaaacaatt cggcggcaaa ctcaagggca
      181 agcgcatgct gggcatcggc atggccatgc caggcccctt cgatgtggaa tcgatgagct
      241 tcgtcggacc caccacactg gaaggctggc gcgggtttcc ggtgctggag cgactgaaca
      301 gggtctcagg cttgccagcc ttcatcgaag tggaccatgc cgcggctgcg ctgggcgaaa
      361 ggctgcatgg cgccggcaag gccttccgcg acttctactt cctctatttt ggcgtcggcc
      421 tgggcggatg catggtgcat gaaggcttgc cctggcgcgg ttcgcatcgc aacgccggcg
      481 agatcgggca tctgccgctg gtccccaatg gcgaaccctg tccttgtggc aatcgcggct
      541 gcctggaacg ctatctctcg ctcgaggcct atggccggcg ggcaacggcg atcggccgcg
      601 aagcctggat cagcgaggcg gcgccgctgt tccgcaacgc agtggcagcc atcgagaacc
      661 tcttcgaccc ggagaccatc atcgtcggcg gcctcgcgga cagcggcctg ctcgacgatc
      721 ttatcgccgc cgcccagccg ctgcccaact cggtctcgca gcgcgcggac cgcgaggtgc
      781 cgcggctgat gcgcgccgac agcggacagg acggcgtgct gcgcggtgcc gcggccctgg
      841 cgattgccgg cgtgctgtcg ccccgcttcg gtttgctctt tgcggaggga gaacgcaccg
      901 aacgcgatcc gatcatcgcc gggagacagg tggcatgagc agcgataccc tgctccgcct
      961 aaaggacatc acgcgcaact tcggcgccat cgaggcgctg cgcggcatca gcttcgaggt
     1021 gcagcgcggc gaggtgctgg cactcctcgg cgacaacggc gccggcaagt cgacgctggt
     1081 gaagatcatc tcgggcgggc tgaagcccac ctcaggctcg ctctatttcg atggacgcga
     1141 aaggaacttc gcctcgccgg ccgacgccaa ggcggagggg atcgagacgg tctatcagga
     1201 cctgtcgctc tgcacgaatg tagacgtcgt ggccaacttc ttcatgggcc gcgaggtcgt
     1261 gaagcggtac ttcggaatac cggtcctgca ggaagccgag atggaggccg ccgtggcccg
     1321 agccatgtcc aatgccggaa cccgcatccc gtccttgcgt accaacgtgg agtatctgtc
     1381 cggcggacag cgccaggcca tcgaactcaa ccgcttcgtc cactggggcg gcaagctggt
     1441 tctgctcgac gaacccttcg cggccctcgg cgtcgagcag acacggcgcg gcctcgagat
     1501 gatccgccgc atcgcggacc agggcatcgg cgtcatcatc atcactcaca tcatggccca
     1561 ggccttccag gttgcggatc gcatcgtggt gatccgccag ggactggtag cg
//
LOCUS       DBNGVR010000362         3728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4454306, whole genome shotgun sequence.
ACCESSION   DBNGVR010000362 DBNGVR010000000
VERSION     DBNGVR010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4454306"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="AC16A0_07650"
     CDS             <1..403
                     /locus_tag="AC16A0_07650"
                     /inference="COORDINATES: protein motif:HMM:NF017470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIR3105246.1"
                     /translation="TRGPFRGARFAPGGIAMDWKYLFTSFEGRIGRQQMWMGIIVLMV
                     VSLVANVLDMLFGTIDVESGIGIIGGLFALVSIYPAVALYAKRWHDRGKSGWWTLILF
                     VPLIGFIWWLVECGFLRGTEGPNQYGPDPVA"
     gene            472..1644
                     /gene="dapE"
                     /locus_tag="AC16A0_07655"
     CDS             472..1644
                     /gene="dapE"
                     /locus_tag="AC16A0_07655"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR3105247.1"
                     /translation="MKNTPTDSVPLLQELIRCKSVTPSEGGALTLLENRLSQAGFVCQ
                     RLIFKDTDTPDVENLFARFGTGAPHLCFAGHTDVVPEGHVDAWSHAPFAADIADGFVY
                     GRGATDMKGSVAAFTAAAIDFVRTAGRFKGSISFLITGDEEGPAINGTVKVLQWMRDN
                     GHVPDHCLVGEPSCADRLGDTIKIGRRGSLSFTVVVEGRQGHSAYPHKADNPVPKLAR
                     FVDRISHAKLDEGNTHFDPSTLAVTSFDVGNAATNVIPSKAMAKFNIRFSTEHAPPSL
                     RDWVNAHIDAVRSELGGTWSASSNEGADAFITEPGAFVGLVQDAVEAETGILPKLSTS
                     GGTSDARFIKDYCPVLEFGPTNATIHQTDERIAIDELRATQTIYHRILTDYFNSQP"
     gene            complement(1628..2365)
                     /gene="truA"
                     /locus_tag="AC16A0_07660"
     CDS             complement(1628..2365)
                     /gene="truA"
                     /locus_tag="AC16A0_07660"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3105248.1"
                     /translation="MPRYKLIIEYDGEPFVGWQFQAAGHSVQGVLEAAVAQINGAPAT
                     VHGAGRTDAGVHALGQVAHVDLERPWDVFKLRNALNANVRPHPVSVLAISEVGEDFHA
                     RFSATRRHYLFRILNRRSPPALDRGKVWWLPVDLDAEAMHAAAQLLVGKHDFSTFRAA
                     QCQAQSPVKTLDRIDVARYGELIELRCDARSFLHNQVRSMVGSLRLVGEGKWRAQDLK
                     RALDARDRASCGPVSPPDGLYLLRVDY"
     gene            complement(2365..3270)
                     /gene="fmt"
                     /locus_tag="AC16A0_07665"
     CDS             complement(2365..3270)
                     /gene="fmt"
                     /locus_tag="AC16A0_07665"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3105249.1"
                     /translation="MRLVFMGTPDFAVETLKALLAAGHEVACVYSQPPRPAGRGMAER
                     PSPVHAHAIAHGIEVRTPVSLKPPEEHLRFAALAADAAVVVAYGLLLPAPVLDAPRLG
                     CFNVHASLLPRWRGAAPIQRAIMAGDAESGVTIMRMSEGLDTGPMCKVGRLPITPQTT
                     AQSLHDALAELGAGLMVEVLAAREIHCVPQPDAGVTYARKIGKTEARIDFARPATDVR
                     NHIHGLSPFPGAWFPVRDTRIKVLLCEVTEGKGPAGSVIDGALTIACGSGAIRALKVQ
                     REGKGVMDSETLLRGFAIPAGTVVG"
     gene            complement(3275..>3728)
                     /gene="def"
                     /locus_tag="AC16A0_07670"
     CDS             complement(3275..>3728)
                     /gene="def"
                     /locus_tag="AC16A0_07670"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3105250.1"
                     /translation="AAVDAEVRKQWDDMLETMYHAPGIGLAAIQIGLPRRLVVIDLAK
                     DGEEKQPLFVANPEIVWKSEAQSDYEEGCLSIPEYYEMVTRPSEIRLRYLDRQGEAQE
                     LHATGLLATCMQHEIDHLDGVLFIDHISRLKRERVIKKFAKAQKLGKL"
BASE COUNT          631 a         1229 c         1226 g          642 t
ORIGIN      
        1 gacccggggg ccttttcggg gggcgcgctt cgcgccagga gggattgcga tggactggaa
       61 gtatcttttc accagtttcg aaggccgcat cggccggcag cagatgtgga tgggcatcat
      121 cgtgctgatg gtcgtcagcc tcgtcgccaa cgtgctcgac atgctgttcg gcaccatcga
      181 cgtcgagtct ggaatcggga tcatcggcgg gctcttcgcg ctggtctcga tctatccggc
      241 cgtcgcgctc tatgccaagc gctggcatga ccgcggcaaa tccggatggt ggacgctcat
      301 cctcttcgtg ccgctgatcg gtttcatctg gtggctggtc gagtgcggct tcctgcgcgg
      361 cacggaaggc cccaaccagt acgggcctga cccggtcgcc tgagggtcat tcttttgtca
      421 acatgcccgg cccctgtggc cgggcatttc gtttccgtct atgctgtccg catgaagaac
      481 acaccgaccg actccgtgcc gctgctgcag gagcttatcc gctgcaagtc cgtaaccccc
      541 agcgaaggcg gcgccctgac attgctggaa aatcgcctgt cgcaggctgg cttcgtctgc
      601 cagcgcctga tcttcaagga tacggacacg ccggatgtgg agaacctctt tgcccgcttc
      661 ggcacgggcg cgccgcatct gtgcttcgcg gggcataccg acgtcgtgcc cgagggccac
      721 gtggatgcct ggtcgcatgc gccctttgcc gccgacatcg ccgatggctt cgtctacggg
      781 cgcggcgcca cggacatgaa gggttccgtc gccgccttca cggccgcggc catcgacttc
      841 gtcagaacgg ccggcagatt caagggttcc atctccttcc tcatcaccgg cgacgaggaa
      901 ggtccggcca tcaacggcac ggtcaaggtg ctgcaatgga tgcgcgacaa cggccatgtg
      961 ccggaccact gcctggtggg cgagccaagc tgcgccgaca ggctgggcga cacgatcaag
     1021 atcggccggc gcggctcgct gagcttcacc gtcgtggtcg agggacgcca gggccattcc
     1081 gcctatccgc acaaggccga caatccggtg cccaagctcg cccgcttcgt cgaccgcatc
     1141 tcgcatgcga agctcgacga gggcaacacg catttcgatc cctcgacgct cgccgtcacc
     1201 agcttcgacg tcggcaatgc cgcgaccaac gtcattccct ccaaggcgat ggcgaagttc
     1261 aacatccgct tctcgacaga gcacgcacca ccgtcgctgc gcgactgggt gaatgcccat
     1321 atcgatgcgg tccggtcgga actgggcggc acctggtcgg cgagcagcaa tgaaggggcg
     1381 gatgccttca tcacggagcc gggcgccttc gtgggactgg tgcaggacgc cgtcgaggcc
     1441 gagaccggca ttctgccgaa gctgtccacc tcgggcggaa cctcggatgc ccgcttcatc
     1501 aaggactatt gcccggtgct ggagttcggc ccgaccaatg ccacgatcca ccagaccgac
     1561 gaacgcatcg caatcgacga gctgcgcgcc acgcagacca tctatcaccg gatcctgacc
     1621 gactatttca atagtcaacc ctgagcagat agagcccgtc cggcggcgag accgggccgc
     1681 agctggcgcg gtcgcgggcg tcgagcgccc gcttcagatc ctgcgcgcgc cacttgcctt
     1741 cgcccacaag gcggagcgag cccaccatcg agcggacctg attgtgcagg aaggagcggg
     1801 catcgcagcg cagttcgatc agctcgccgt agcgggcgac gtcgatgcgg tcgagcgtct
     1861 tgaccggcga ctgggcctgg cactgcgctg cccggaaggt ggagaaatca tgcttgccga
     1921 ccagaagctg ggccgcggca tgcatggcct cggcgtcgag atcgaccggc agccaccaga
     1981 ccttgccgcg atcgagcgcc ggcgggctgc gccggttcag gatgcggaac aaatagtgcc
     2041 ggcgcgtcgc cgagaagcgg gcgtggaaat cctcgcccac ctcggagatc gccaaaacgc
     2101 tcaccggatg cggccttaca tttgcattga gcgcattgcg cagcttgaag acgtcccatg
     2161 gtctctcgag atcgacatgg gcaacctggc ccagcgcgtg aacgccggca tcggtgcggc
     2221 cggcgccatg cacggttgcg ggcgcaccgt tgatctgcgc caccgcggcc tcaagcacgc
     2281 cctgcaccga atggcccgcc gcctggaact gccagccgac gaagggttcg ccgtcatatt
     2341 cgatgatgag cttgtagcgc ggcatcagcc gaccactgtt ccggcgggga tggcgaagcc
     2401 gcggagcagc gtctcagaat ccatgacgcc cttgccttcg cgctgaacct tgagggcgcg
     2461 aatggcgccg ctgccgcagg cgatggtcaa ggcgccgtcg atgacggagc cggcgggccc
     2521 cttgccctcc gtcacttcgc agagcagcac cttgatgcgc gtgtccctga ccggaaacca
     2581 ggcgccggga aagggcgaca gcccgtgaat gtgattgcgg acgtcagtcg ccggccgtgc
     2641 gaagtcgatg cgcgcctcgg tcttgccgat cttgcgcgca taggtcacgc cggcatcggg
     2701 ctgcggcacg caatgaatct ccctcgccgc cagaacctcg accatcagcc cagcgccaag
     2761 ttcggccagg gcgtcatgca gggattgtgc cgtggtctgc ggcgtgatcg gcagcctgcc
     2821 gaccttgcac atgggtccgg tgtcgaggcc ctccgacatg cgcatgatgg tgacgcccga
     2881 ctcggcgtcg cctgccatga tggcgcgctg gatgggcgcc gcgccgcgcc agcgcggcag
     2941 caacgaggca tggacgttga aacatcccag ccgcggcgcg tcgagaacag gcgccggcag
     3001 gagcaggcca taggcgacga cgacagctgc gtcggcagcc agcgcggcga aacgcagatg
     3061 ctcctcgggg ggcttcaggg agacgggcgt gcggacttcg atgccatgcg caatggcgtg
     3121 ggcatggacg ggcgacgggc gctcggccat gccgcgcccg gccgggcgtg gcggctggga
     3181 atagacgcag gccacctcat ggccggcggc cagcagggct ttcagcgtct cgaccgcgaa
     3241 gtccggcgtg cccatgaaga ccaggcgcat ggcctcagag ctttccgagt ttctgcgcct
     3301 tggcgaattt cttgatgacg cgctcgcgtt tcagacgcga gatgtggtcg atgaagagca
     3361 cgccgtccag gtggtcgatc tcgtgctgca tgcaggtggc gagcaggccg gtggcgtgga
     3421 gctcctgtgc ctcgccctgc cggtcaagat agcgcagcct gatctcggat ggccgcgtga
     3481 ccatctcgta gtattcgggg atcgagagac agccctcctc gtaatcgctc tgggcctcgc
     3541 tcttccagac gatttccgga ttggccacga agagcggctg tttttcctcg ccgtccttgg
     3601 ccagatcgat gaccacaagg cgtcttggca ggccgatctg gatggcagcc agcccgatgc
     3661 ccggcgcgtg gtacatggtc tcgagcatgt cgtcccactg cttgcgcacc tcggcatcga
     3721 ccgccgcc
//
LOCUS       DBNGVR010000363         3069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2490585, whole genome shotgun sequence.
ACCESSION   DBNGVR010000363 DBNGVR010000000
VERSION     DBNGVR010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2490585"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC16A0_07675"
     CDS             <1..250
                     /locus_tag="AC16A0_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR3105251.1"
                     /translation="HFFGTPHTLARYETAFYQPMISNWDNYDTWLERGRETAPVKASR
                     VWKQMLASYERPPLDPAVDAALKEFVARRKRHYLGDRA"
     gene            complement(229..840)
                     /locus_tag="AC16A0_07680"
     CDS             complement(229..840)
                     /locus_tag="AC16A0_07680"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR3105252.1"
                     /translation="MRLTRGEWVIAIFTAVYVAAFLAYFIMIADQEFIGYLATMVILV
                     GLVTWSHQLMRFPLTLLWALALWGLAHMAGGSLQVWGKVLYNLVLLPIAGDGELRILK
                     YDQLVHFYGFAVTAWMLWFILQKRAPQLRRTLAVYAFPALASMGLGAVNEIVEFSAVM
                     LVPHTNVGGYYNTALDLVFNALGAIFAMLVCAAWEWVQARSPR"
     gene            complement(857..1303)
                     /locus_tag="AC16A0_07685"
     CDS             complement(857..1303)
                     /locus_tag="AC16A0_07685"
                     /EC_number="2.8.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102100 - mycothiol-arsenate ligase
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate-mycothiol transferase ArsC"
                     /protein_id="HIR3105253.1"
                     /translation="MPGDLPSAVLFACTQNVIRSPMAAAIMKHFYGHKVYVASCGVKP
                     GQPDPFVNAVMEEVGIDMGKYRPQGFDDLEDSSFDLVISLSPEAHHRALELTRTMAID
                     TEYWPTMDPSITMGSREQILDSYREVRDTLTRKIRQRFGSVAARGV"
     gene            complement(1312..1791)
                     /locus_tag="AC16A0_07690"
     CDS             complement(1312..1791)
                     /locus_tag="AC16A0_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HIR3105254.1"
                     /translation="MNDKPRTRLLAVELDSTIGPGPSPEAEHERRVAIYDLVEENSFC
                     LAAEPTGPYRLRLSTADGRLIFDVLNEREERLSLIGLSMSPFRRIVKDYYMICESYYQ
                     AIRTATPSQIETIDMARRGLHNEGSELLRERLNGKVEVDFDTARRLFTLVCVLHWRG"
     gene            complement(1825..2256)
                     /locus_tag="AC16A0_07695"
     CDS             complement(1825..2256)
                     /locus_tag="AC16A0_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HIR3105255.1"
                     /translation="MLKLAALDAEDLAVLSAHMQDALVKAGDLAFNRSRRQFALVANR
                     FAWDADEDRQRRRSGLHFDRVLSVRSRGIPRHDADAVLSLLSIGFEMGDAPAGDVVLT
                     FSGGGNIRLTVECIEAQLRDLGPVWQASSVPSHDDTDGKAP"
     gene            complement(2262..>3069)
                     /locus_tag="AC16A0_07700"
     CDS             complement(2262..>3069)
                     /locus_tag="AC16A0_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105256.1"
                     /translation="HVTVEGKRVGQDEFLGETMRLESPTVVDTTAPYELVSKMRASFW
                     VIGPLLARMHRAKVSLPGGCAIGTRPVDFFLMGLQALGAVIDIEAGYVVAKAPRGLKG
                     ADAGHLGAVLDVMRQAGIGVDVTNEGIRIRRNGQALDPVRVETQPFPGFPTDCQAQLM
                     ALMTMANGTSMIRETIFENRFMHVQELARLGADIQLHGDTAEVRGVKKLTGAPVMATD
                     LRASVSLVIAALAAEGETTINRVYHLDRGFETLEEKLQACGADVSRISGN"
BASE COUNT          563 a          975 c         1010 g          521 t
ORIGIN      
        1 acatttcttc ggaacgccgc acactctggc gcgctacgag accgcattct accagccgat
       61 gatctccaac tgggacaatt atgacacctg gctggagcgc ggccgcgaga cggcgcctgt
      121 gaaagccagc cgggtctgga aacagatgct cgccagttac gaaaggccac cgctcgatcc
      181 ggccgtcgac gcggcgctga aggagttcgt cgcccgccgc aagcgccact atctcggcga
      241 ccgcgcctga acccattccc atgccgcgca gaccagcatg gcgaagatcg cccccagggc
      301 attgaacacg aggtcgagtg ccgtgttgta gtagccgcct acgttggtgt gcggcaccag
      361 catcacggcg gagaactcga cgatctcgtt gacggcgcca agccccatgc tggccagggc
      421 cgggaaggca tagacggcca gggtccggcg cagctgcggt gcccgcttct gcaggatgaa
      481 ccacagcatc cacgccgtca ccgcgaagcc gtagaaatgc acgagctggt catacttgag
      541 gatccggagt tcgccgtctc cggcaatcgg gagaaggacc aggttgtaga gaaccttgcc
      601 ccaaacctgc aggctgccgc cggccatatg cgcaagaccc cagagcgcaa gggcccagag
      661 cagggtcagg ggaaagcgca tcagctggtg gctccaggtc acgaggccga cgaggatcac
      721 catggtggcc agatagccga tgaactcctg atcggcgatc atgatgaagt atgcgaggaa
      781 ggccgcgacg tagacagcgg taaagatcgc gatcacccac tcgccgcgcg tcaatcgcat
      841 gatggccgct ccatcatcac acgccgcgcg cagcgacgct gccgaagcgc tggcggatct
      901 tccgtgtgag cgtatcgcgt acctcccgat agctgtcgag gatctgttcg cggctcccca
      961 tggtgatcga ggggtccatg gtcggccagt actccgtgtc gatggccatg gtgcgcgtca
     1021 gctccagcgc gcggtgatgc gcctcgggcg agagggagat gacgagatcg aaactggaat
     1081 cctcgaggtc atcgaaaccc tgcggacggt acttgcccat gtcgatgcct acttcctcca
     1141 tcacggcgtt gacgaaggga tcgggctggc ccggcttcac cccgcaggat gcgacataga
     1201 ccttgtggcc atagaaatgt ttcatgatcg ctgcagccat gggcgaacgg atgacgttct
     1261 gggtgcaggc aaagaggacg gcgctgggaa gatcgcccgg catgcgatgg gtcagccccg
     1321 ccagtgcaag acgcagacca gcgtgaacag gcgccgcgcc gtgtcgaaat ccacctccac
     1381 ctttccgttc aaccgttcgc gcagcaattc gctgccttca ttgtgcaatc cccgccgcgc
     1441 catgtcgatg gtctcgatct gcgacggcgt ggcggtgcgg atcgcctggt aatagctctc
     1501 acagatcatg tagtagtcct tcacgatgcg gcggaagggc gacatcgaga ggccgatcag
     1561 cgaaaggcgt tcctcccgct cgttgagcac gtcgaagatc agccggccat cggccgtgga
     1621 cagccgcagg cgataggggc ccgtgggctc ggcggcaagg cagaaactgt tctcttcgac
     1681 caggtcatag atggcgaccc ggcgttcgtg ctcggcctcg ggcgaggggc ccggtccgat
     1741 ggtcgaatca agttcgaccg ccagcagccg tgtcctgggt ttgtcgttca tggcacgaca
     1801 atagccgggc ggccgccgtg actgctaggg ggctttgccg tccgtgtcgt catgactggg
     1861 aacgctactg gcctgccaga caggacccag gtccctgagc tgcgcctcga tgcattccac
     1921 cgtcagccgg atgtttcctc cgccggagaa ggtgagcacc acgtcacccg ccggtgcgtc
     1981 gcccatttcg aacccgatgg agagcagcga caggacggca tcggcgtcgt gccgggggat
     2041 tccgcgtgac ctgaccgaca ggacgcggtc gaaatgaagt ccgctgcgcc ggcgttgccg
     2101 gtcctcgtcg gcgtcccagg caaagcggtt ggcgaccagg gcaaactgcc ggcggctgcg
     2161 gttgaaggcc agatcgccgg ccttgaccag cgcgtcctgc atgtgggcag acagcacggc
     2221 aaggtcttcg gcatcaaggg cggcaagctt gagcattacc gtcagttccc ggatatcctg
     2281 ctcacgtctg caccgcaggc ctggagcttt tcctccagtg tctcgaatcc gcggtcgagg
     2341 tgatagacgc ggttgatggt ggtctcgcct tcggcggcaa gtgcggcgat gaccagcgag
     2401 accgaggcgc ggaggtcagt ggccatcacc ggcgctccgg tgagcttctt cacgccgcgg
     2461 acttcggccg tatcgccatg gagctggatg tcggctccca gccgggccag ttcctgcaca
     2521 tgcatgaagc ggttctcgaa gatggtctcg cggatcatgc tggtgccgtt ggccatggtc
     2581 atcagcgcca ttagctgggc ctggcaatcg gtggggaagc cggggaaggg ctgggtctcg
     2641 accctgaccg gatccagtgc ctggccattg cggcggatgc ggatgccttc gttggtcaca
     2701 tcgacgccga tcccggcctg gcgcatcaca tcgagaaccg cgcccagatg cccggcgtcg
     2761 gcgcccttca gaccgcgcgg cgccttggcg acgacgtagc cggcctcgat gtcgatcacg
     2821 gcgccgagcg cctgcaggcc catgaggaag aaatcgaccg gccgcgtgcc gatcgcgcag
     2881 ccgcccggca gcgacacctt cgcccggtgc atgcgggcga gcagcggccc gatcacccag
     2941 aagctggcgc gcatcttcga gacgagctcg taaggcgcgg tggtgtcgac gacggtcggc
     3001 gattcgagcc gcatcgtctc gcccaggaac tcgtcctggc cgacgcgctt gccctcgacg
     3061 gtgacgtgc
//
LOCUS       DBNGVR010000364         8691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_922814, whole genome shotgun sequence.
ACCESSION   DBNGVR010000364 DBNGVR010000000
VERSION     DBNGVR010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_922814"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16A0_07705"
     CDS             <1..410
                     /locus_tag="AC16A0_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3105257.1"
                     /translation="LRPLYDALNDCLTPKEVARRMENGEIEIAPLAFMRGRTLSSAFV
                     ILDEAQNTTPQQMKMFLTRLGEGSRMVVTGDPTQVDLPNGISSGLADAVATLKGVDSV
                     AAVHFANADIVRHALVGRIVAAYDKKTAKPRRT"
     gene            407..871
                     /gene="ybeY"
                     /locus_tag="AC16A0_07710"
     CDS             407..871
                     /gene="ybeY"
                     /locus_tag="AC16A0_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3105258.1"
                     /translation="MSLEIDVEEAGWAELAGLEELAATACAAVFAAQGKRPDDYDVSL
                     VFSSDEAVRALNAAWRGKDKATNVLSFPAEDMPLPKGEPHPLGDIILAHGVVVREAAE
                     QGKTLRDHAAHLMIHGLLHLLGHDHEDDGEAAAMEALEIRILKGLGITNPYE"
     gene            864..1880
                     /locus_tag="AC16A0_07715"
     CDS             864..1880
                     /locus_tag="AC16A0_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3105259.1"
                     /translation="MSDSDSSSAATSSDARRDGLWRRLRTRFGRKSEMGLRESLEGAL
                     ETHGAQNPGETVRAEAKSMMLNIIEFSNLRVDDVMVPRVDIVAIDEAASMRELLAKFI
                     DANHSRLPVYRETLDGITGMVHVKDFMRWMDAKGVKRRKAKTDKSEKPEKLPAAGLSI
                     AAAELAVAVKQSGLHRDVLFVPPSMPASDLLVRMQASHIHLAIVIDEYGGTDGLVSIE
                     DLVEVIVGDISDEHDTEEGLQIKAVGEGIYMADGRVDIAALEDLVKIDLLSDEEEEEA
                     DTLAGLIFKIAGRVPARGEIIRHSSGLEFEILDSDPRRVKKLKVDVKKVRLPSDEAAE
                     DASG"
     gene            complement(1881..2870)
                     /locus_tag="AC16A0_07720"
     CDS             complement(1881..2870)
                     /locus_tag="AC16A0_07720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3105260.1"
                     /translation="MSKRKPLVMVTRKLPDVVETRMMELFNTRLNANDEPMSKAELIE
                     AAKSAEVLVPTVTDRIDKAILNQAGDQLKLIANFGTGVDNIDVETALSRGITVTNTPG
                     VLTEDTADMTMALIMAVARRLIEGAKVIGPDNSWSGWSPTWMLGSRIWGKRLGIIGMG
                     RIGTAVARRAKAFGLQIHYHNRKPVHAKVEEELEATYWESLDQMLARMDIVSVNCPHT
                     PATYHLLSARRLKLMKKEAIIVNTARGEVIDENALARMLEAGELGGAGLDVFEHEPAV
                     NPRLLKAKKAVLLPHMGSATIEGRIDMGEKVIINIKTWVDGHNPPDRVLPAML"
     gene            3052..3651
                     /locus_tag="AC16A0_07725"
     CDS             3052..3651
                     /locus_tag="AC16A0_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3105261.1"
                     /translation="MGMASNRRIGTVVLAVACLAVVAGTGWSLFGKRSDSQAGTAPGE
                     TTASIGSAARSLGPSGLPIPRFVSLKAEKVNVRRGPSSDHAVSWVFNRKGMPVEIVAE
                     FENWRRIRDSDGEEGWILQNMLSGKRTVVVAPWRQGQAMSLFREARAEAGLVAKLSAG
                     VVAEVEACNGTWCRITAAGYDGWVEQSMLWGVYPGEAVD"
     gene            3945..5660
                     /gene="rpsA"
                     /locus_tag="AC16A0_07730"
     CDS             3945..5660
                     /gene="rpsA"
                     /locus_tag="AC16A0_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3105262.1"
                     /translation="MAKSAVSLNPTRDDFEALLAESYADHPATEGSVVKGKVVAIEND
                     FAIIDVGLKTEGKIATKEFAQPGKASEIKVGDTVEVYLERIENAMGEAVLSRDKARRE
                     EAWTRLEAMSAKNERVDGVIFGRVKGGFTVDLGGAVAFLPGSQVDVRPIRDVTPLMNV
                     SQPFQILKMDRRRGNIVVSRRAIMEETRAEQRSELVQNLAEGQVVDGVVKNITDYGAF
                     VDLGGIDGLLHVTDIAWKRVNHPSDVLHVGQTLKVQIIRINPETQRISLGMKQLDKDP
                     WEIVAERYPIGARVKGKVTNITDYGAFVELEDGIEGLVHVSEMSWTKKNVHPGKILST
                     SEEIDVQVLEIDAAKRRISLGLKQTQNNPWATFASKYPNGSTVEGEVKNITEFGLFIG
                     LDGDVDGMVHLSDIDWKRAGEEAIKDFKKGDIVKAKVLDVDQEKERISLGIKQLGTDP
                     VSTAAGVKKGDTVTCEVVKVEDNGLEVRVAGTDMSAYVKRADLARDRSEQRPERFAVG
                     EKFDAKVTLFDAKARKMNVSIKALEIAEEKEAVAQYGSSDSGASLGDILGAALKKKDA
                     EAKSD"
     gene            5728..6666
                     /gene="sppA"
                     /locus_tag="AC16A0_07735"
     CDS             5728..6666
                     /gene="sppA"
                     /locus_tag="AC16A0_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3105263.1"
                     /translation="MITEAELVVDRRRMKRRLTLWRIAAVLLAVVAIAALAWPAGGPR
                     SFENHIARVRIDGLITGDQKTLDLLDEISRSRRVQGVILRIDSPGGTTAGSEALYEQI
                     RKLAKDKPVAAVMDTVAASGGYIAAIAADRIVARGNTITGSIGVIFSFPEVSQLLATL
                     GIKMEEIKSGDLKAEPSPYKPVTEKAREVSQAMVQDGFEWFTGLVAERRGLPIEQVRT
                     LSDGRVYTGRQALKVKLIDEIGGEEAAVKWLTEDRKLAASLRIVDWAPSRSAGATGLG
                     FAVGDTLLRVLGFERLQAAAERAQLDGLLVLWHPDL"
     gene            6696..6983
                     /locus_tag="AC16A0_07740"
     CDS             6696..6983
                     /locus_tag="AC16A0_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR3105264.1"
                     /translation="MIKSELVQMIADKNPHLYHRDIERIVNTVLDEIISAMKNGDRVE
                     LRGFGAFSVKSRNARMGRNPRTGTAVEVVAKRVPFFKTGKELRERLNAPQE"
     gene            6980..7324
                     /locus_tag="AC16A0_07745"
     CDS             6980..7324
                     /locus_tag="AC16A0_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR3105265.1"
                     /translation="MTFRSLLNWAIGLPIAAAAVGFAVANRQWVSVSLDPIHRDQPFA
                     SIQMPLWVLFFCGIFVGIFVGWVAAWFAQGKWRKAAKEARIELVRVHTEHERMKREHQ
                     SQAVVPVQDGSA"
     gene            7321..8244
                     /gene="lhpI"
                     /locus_tag="AC16A0_07750"
     CDS             7321..8244
                     /gene="lhpI"
                     /locus_tag="AC16A0_07750"
                     /EC_number="1.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-
                     carboxylate reductase"
                     /protein_id="HIR3105266.1"
                     /translation="MRIFAADEVARLAPYRDIVEALRQGFRDEIRTPVRHHHDLSPQA
                     ILLLMPAWSRQFTGLKTVVFKSDNAAKGLPTIQASYLLIDNATGATVAMMDGGEITRR
                     RTAAASALAADYLARPDASTMTLVGAGALGAHFVRAHASVRPIRRVFIFSRTRAKSEA
                     LAAELAGDGLAAIVTDDLASAVRQSDLVSCVTTSTAPIVKGEWLRPGTHVDLAGAFKP
                     SMRETDGEAVARASVYVDTRDGALAEAGGLLQARDEGRFDFASVKGDLFDLCRGKAAG
                     RASSGEITLFKSCGTAIEDLATAIMVYQKAA"
     gene            8241..>8691
                     /locus_tag="AC16A0_07755"
     CDS             8241..>8691
                     /locus_tag="AC16A0_07755"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HIR3105267.1"
                     /translation="MTAASPLCIAIDTPDLDRATSLARELRPSIGYVKLGMEFFYAHG
                     AAGYARVAESGVPIFLDLKLHDIPNTVAEGLRSLLRLDPRPAIVNVHATGGLDMMKAA
                     ADAVAGQCKLIAVTILTSLSDDDIWAAGFETARNTEGHVRSLAALARS"
BASE COUNT         1545 a         2851 c         2799 g         1496 t
ORIGIN      
        1 acctgcgccc gctctacgac gcgctgaatg attgcctgac gcccaaggaa gtcgcccgcc
       61 gcatggaaaa cggcgagatc gagattgcgc cgctcgcctt catgcgcggc cggaccctgt
      121 cgtcggcctt cgtcatcctg gatgaagcgc agaatacaac cccgcagcag atgaaaatgt
      181 tccttacacg cctcggcgag ggcagccgca tggtggtgac cggcgatccc acccaggtcg
      241 acctgccgaa cgggatctcc tcgggacttg ccgatgcggt ggccacgctc aagggcgtgg
      301 acagcgtcgc cgcggttcac ttcgccaatg ccgatatcgt gcgccatgcg ctggtcggcc
      361 gtatcgtcgc ggcctacgac aagaagacag caaaaccgcg ccgcacatga gtctcgagat
      421 cgatgtcgag gaggccggct gggctgagct cgccggtctc gaggagctgg cggcaacggc
      481 ctgcgctgcc gttttcgcgg ctcagggcaa acggcccgat gactatgacg tgagtctcgt
      541 cttctcgagc gacgaagccg tccgtgcact gaacgccgcg tggcgcggca aggacaaagc
      601 caccaatgtc ctgtccttcc ccgcagaaga catgcccctg cccaagggcg agccgcatcc
      661 gctgggcgac atcatcctgg cccatggcgt ggtcgtgcgg gaagccgccg aacagggcaa
      721 gacattgcgg gatcatgccg cacaccttat gatccacggg cttctgcacc tgctcggtca
      781 tgaccatgag gacgatggcg aggccgcagc catggaagcg ctcgagattc gaattctgaa
      841 aggacttggt atcaccaacc cctatgagtg acagcgacag tagcagcgcc gcgacgtcaa
      901 gcgacgcgcg gcgtgacggt ctgtggaggc gcctgcgcac gcgcttcggc cgcaagagtg
      961 aaatggggct gcgcgaaagc ctcgagggtg cgctcgagac ccatggcgcg cagaaccccg
     1021 gcgagacggt acgcgcggaa gccaagtcga tgatgctcaa catcatcgag ttctccaatc
     1081 tccgcgtcga cgacgtcatg gtgccgcgcg tcgatatcgt ggccatcgac gaggcggcca
     1141 gcatgcgcga acttctggcc aagttcatcg acgccaatca ctcgcgcctt cccgtctatc
     1201 gcgagacgct cgacggcatc accggcatgg tgcacgtcaa ggacttcatg cgctggatgg
     1261 atgcaaaggg cgtcaagcgc cgcaaggcca agaccgacaa gtcggagaag ccggagaaac
     1321 tgccggcggc cggtctgtcg atcgccgcag ccgaactcgc tgtcgccgtc aagcagtcgg
     1381 ggctgcaccg cgacgttctc ttcgtgccgc cgtccatgcc agcctctgac ctgcttgtcc
     1441 gcatgcaggc gagccacatt cacctggcca ttgtcatcga cgagtatggc ggcaccgacg
     1501 gcctcgtctc gatcgaagac ctggtcgagg tgatcgtcgg cgacatttcc gacgagcacg
     1561 ataccgagga gggcctgcag atcaaggctg tcggcgaagg catctacatg gccgacggcc
     1621 gcgtcgacat cgccgctctc gaggatctgg tgaagatcga tctcttgtcc gacgaagagg
     1681 aggaggaggc cgatacgctg gcaggcctca tcttcaagat tgccggccgc gtgccggcca
     1741 ggggcgagat catccgccac agcagcggac tggagttcga gatcctcgac tccgatccgc
     1801 gccgcgtgaa gaagctcaaa gtcgacgtca agaaggtcag gctgccgagc gacgaggctg
     1861 ccgaggacgc ctcgggctga tcagagcatc gccggcagga cgcggtcggg cggattgtgg
     1921 ccgtcgaccc aggtcttgat gttgatgatg accttctcgc ccatgtcgat gcggccttcg
     1981 atggtggcgg agcccatgtg cggcagcagc accgccttct tggccttgag cagccgcggg
     2041 ttgaccgccg gctcatgttc gaagacgtcg aggccggcac cgcccagctc gcccgcctcc
     2101 agcatccgcg ccagggcatt ctcgtcgatc acctcgccgc gcgcggtgtt cacgatgatg
     2161 gcttccttct tcatcagttt caggcgccgc gccgagagca ggtgataggt ggccggcgta
     2221 tgcgggcaat tgaccgagac gatgtccatg cgcgccagca tctggtcaag gctttcccag
     2281 taggtggctt cgagttcctc ctcgactttc gcgtgcaccg gcttgcggtt gtgatagtgg
     2341 atctgcagtc cgaaggcctt ggcccgccgg gccacggcgg tgccgatgcg gcccatgccg
     2401 atgatgccga ggcgcttgcc ccagatacgg ctgccgagca tccaggtggg cgaccagccg
     2461 gaccagctgt tgtcggggcc gatgaccttc gccccctcga tgaggcgccg cgccacggcc
     2521 atgatgaggg ccatggtcat gtcggcagtg tcctcggtca gaacgcccgg cgtattggtg
     2581 accgtgatgc cgcgcgacag ggctgtctcg acatcgatgt tgtcgacgcc cgtgccgaaa
     2641 ttggcgatca gtttcagctg atcgccggcc tggttgagaa tggccttgtc gatgcgatcg
     2701 gtcacggtgg gaaccagcac ctcggccgac ttcgccgcct cgatcagttc ggccttgctc
     2761 atgggctcgt cgttggcgtt gaggcgcgtg ttgaaaagtt ccatcatccg cgtctcgacc
     2821 acgtcgggga gtttccgcgt caccatgacg aggggtttgc gcttgctcat tccgggccct
     2881 gcctccctaa acgctctttc acacaatctt aaccctgaca cggcaaccgt gtcatgaagt
     2941 atcgggagag gcggtttcca cccgccgcaa ccatggtgta gacttgattc gtgtctagca
     3001 ggacggcggt gttgcgcaag tgaccgcgaa gggcgcgggg cacggagatc gatgggtatg
     3061 gcgtcaaacc gcagaattgg caccgttgtg ctggccgtgg cctgtctcgc cgttgtggcg
     3121 ggaaccggct ggtcgctttt cggcaagcgc agcgattcgc aggcgggtac cgccccgggc
     3181 gaaaccacgg cctcgatcgg ttccgccgcc cgttcgctgg gtccttccgg ccttcccatc
     3241 ccgcgcttcg tgtccctcaa ggccgagaag gtcaatgtcc gtcgcggtcc gtccagcgat
     3301 catgccgtgt cctgggtctt caaccgcaag ggcatgccgg tcgagatcgt ggccgagttc
     3361 gagaactggc ggcggatccg cgacagcgac ggggaagaag gctggatcct gcagaacatg
     3421 ctgtcgggca agcggacggt ggtggtcgcc ccctggcgac agggccaggc catgtccctc
     3481 ttccgggaag cccgggccga agcggggctg gtggcgaagc tctccgccgg cgtggtggcc
     3541 gaggtcgagg cctgcaacgg gacatggtgc cggatcacgg cggccggcta cgacggatgg
     3601 gtcgagcaat ccatgctctg gggcgtctat cccggcgaag ccgtcgactg agcgcggctg
     3661 tccgtccttg ccggcttttc cttgcccgta ggggcgtttt ccactatagg cagcggcgtt
     3721 ccaggctgcg ccatgatgcg cggaacatga accgggatgc cgtggcatcc caaattgctt
     3781 cagggcgact gtcgaatacc gcttgcctgg gcggctgtcc ggaaccctga aacgcgtcgt
     3841 ggtttgcctg ccggtcccct ttttggaaac aacggggccg cgccggacgt cctagcgggt
     3901 tttccgccgg gccagccccg atttttggaa ggaacgtgaa ccctatggcg aaatccgccg
     3961 tgtccctcaa tcccacccgt gacgatttcg aagccctcct tgccgagagc tatgccgacc
     4021 atcccgctac cgaaggatcg gtggtcaagg gcaaggtcgt tgccatcgaa aacgattttg
     4081 cgatcatcga cgtcggcctc aagactgaag gcaagatcgc gaccaaggaa ttcgcccagc
     4141 cgggcaaggc ctccgagatc aaggtcggcg acaccgtcga ggtctatctg gagcgcatcg
     4201 aaaacgccat gggcgaggcc gtcctgtcgc gcgacaaggc gcgccgcgaa gaggcctgga
     4261 cccgtctcga ggccatgagc gcgaagaacg agcgcgttga cggcgtcatc ttcggccgcg
     4321 tcaagggcgg cttcaccgtc gaccttggcg gtgccgtggc cttcctgccc ggcagtcagg
     4381 tggacgtgcg gccgatccgc gacgtgaccc ccttgatgaa cgtctcgcag cctttccaga
     4441 ttctcaagat ggaccgccgc cgcggcaaca tcgtggtatc gcgccgcgcc atcatggaag
     4501 agacccgcgc cgagcagcgc tcggaactcg tgcagaacct cgccgagggc caggtggtgg
     4561 atggcgtggt caagaacatc accgactacg gcgccttcgt ggacctgggc ggcatcgacg
     4621 gcctgctcca tgtcaccgac attgcctgga agcgcgtcaa tcacccctcc gacgtgctgc
     4681 atgtcggcca gaccctcaag gtgcagatca tccgcatcaa tccggagacc cagcgcatct
     4741 cgctcggcat gaagcagctc gacaaggatc cgtgggagat cgtggccgag cgctatccga
     4801 tcggcgcgcg cgtcaagggc aaggtcacca acatcaccga ctacggcgcc ttcgtggagc
     4861 tggaagacgg catcgagggc ctcgtccatg tctccgagat gagctggacc aagaagaacg
     4921 tgcatccggg caagatcctc tcgacctccg aggagatcga cgttcaggtg ctcgagatcg
     4981 acgccgccaa gcgccgcatc tcgctgggcc tcaagcagac ccagaacaat ccctgggcca
     5041 ccttcgcgtc caagtacccc aacggctcca ccgtcgaggg cgaggtcaag aacatcaccg
     5101 aattcggctt gttcatcggg ctcgatggcg atgtcgacgg catggtccat ctctccgaca
     5161 tcgactggaa gcgtgcgggc gaagaagcga tcaaggactt caagaagggc gacatcgtca
     5221 aggccaaggt gcttgacgtc gaccaggaga aggagcgcat ctcgctgggc atcaagcagc
     5281 tcggcaccga cccggtctcc accgcggccg gcgtcaagaa gggcgacacc gtcacctgcg
     5341 aagtggtcaa ggtcgaggac aatggccttg aggtccgcgt cgccggcacc gacatgtcgg
     5401 cctacgtgaa gcgcgccgat ctggcccgtg accgttccga gcagcgcccc gagcgtttcg
     5461 ccgtgggcga gaagttcgat gccaaggtca cgctcttcga cgccaaggcc cgcaagatga
     5521 acgtctccat caaggcgctg gaaatcgccg aggagaagga agccgtggcg cagtacggtt
     5581 cttccgactc gggcgcctcg ctgggcgaca ttcttggcgc cgcgctgaag aagaaggacg
     5641 cggaagccaa gtccgactga cgttgccgac gcgggccgga atgtctccgg cccgcgcatt
     5701 ctctaacatg ccgtgccgga gcccgcgttg atcaccgaag ccgaactcgt cgtcgaccgc
     5761 cggcggatga agcgccgcct caccctctgg cgcatcgcag ccgtgctgct ggcggtcgtg
     5821 gcgattgccg cgctggcctg gcctgcgggc ggcccgcgga gtttcgagaa ccatatcgcc
     5881 cgcgtgcgca tcgacggatt gatcaccggc gaccagaaga ctctcgacct tctcgacgaa
     5941 atctcgcggt cgcgccgcgt ccagggcgtc atcctgcgga tcgacagccc gggcggcacc
     6001 acggccggct ccgaggcgct ctacgaacag attcgcaagc tggccaagga caagcccgtg
     6061 gcggcagtca tggatacggt cgcggcctcg ggcggctaca ttgccgcaat cgccgccgac
     6121 cgcatcgtgg cgcgcggcaa caccatcacc ggttcgatcg gcgtcatctt cagcttcccc
     6181 gaggtctccc agctgctcgc cacgctcggc atcaagatgg aagagatcaa gtcgggcgac
     6241 ctcaaggccg agccgtcgcc ctacaagccc gtcaccgaga aggcccgcga agtctcccag
     6301 gccatggttc aggatggttt cgagtggttc accggcctcg tcgcggagcg ccgcggcctt
     6361 cccatcgaac aggtgcgcac gctctccgac ggccgcgtct atacgggccg ccaggcgctg
     6421 aaggtcaagc tcatcgacga gatcggcggc gaggaggcgg cggtgaaatg gcttaccgaa
     6481 gaccgcaagc ttgccgcgag cctcaggatc gtcgactggg cgccatcgcg cagcgccggc
     6541 gccactgggc tcggcttcgc ggtgggcgac acgctgctcc gggtgctggg cttcgaacgg
     6601 ctgcaggcgg cggccgaacg ggcgcagctt gacgggcttc ttgtgctttg gcaccctgat
     6661 ctctgataga agctgccgta cggaggcccc cgcccatgat caagtccgaa ctcgtgcaga
     6721 tgatcgccga caagaacccc cacctctatc accgcgacat cgagcggatc gtgaataccg
     6781 tgctcgacga aatcatcagc gccatgaaga atggcgaccg ggtggagctg cgcggttttg
     6841 gcgccttttc ggtcaagtcc cgcaatgccc gcatgggccg caatccgcgc accggcacgg
     6901 ccgtcgaggt ggtggccaag cgcgtgccct tcttcaagac cggcaaggaa ctgcgcgagc
     6961 gcctgaacgc cccgcaagaa tgacctttcg cagccttctc aactgggcca tcggccttcc
     7021 catcgcggct gccgccgtgg gctttgccgt ggccaaccgg cagtgggtct ccgtctcgct
     7081 cgatcccatt caccgcgacc agcccttcgc ctccatccag atgccgctct gggtcctgtt
     7141 cttctgcggc atcttcgtcg gcatcttcgt gggctgggtg gccgcctggt tcgcgcaggg
     7201 caaatggcgc aaggccgcca aggaggcccg catcgagctg gtgcgcgtcc ataccgagca
     7261 tgagcgcatg aagcgcgaac accagtcgca agccgtggtt cctgtccagg acggcagcgc
     7321 gtgaggatct ttgcggctga tgaagtggcg cgcctcgcgc cctaccgcga cattgtcgag
     7381 gcgctgcgcc agggttttcg cgacgagatc cggacgcccg tccgccatca ccacgatctt
     7441 tcgccccagg ccattctcct gctcatgccg gcctggtcca ggcagttcac cggtctcaag
     7501 acggtggtct tcaagtccga caatgccgcg aaaggcctgc ccaccatcca ggcgagctat
     7561 ttgctgatcg acaatgcaac gggcgcgacc gttgccatga tggatggcgg cgaaatcacc
     7621 cgccgccgca cggccgccgc ttccgccctg gccgccgact atctggcccg cccggacgca
     7681 agcacgatga cgctggtggg ggcgggcgcg ctgggcgctc actttgtacg cgctcatgcc
     7741 agcgtccggc cgatccgcag ggtcttcatc ttcagccgga cccgtgccaa gtcggaagcg
     7801 cttgccgccg agctggcggg agacgggctc gcggcgatcg tcaccgacga tcttgcctcg
     7861 gccgtccgcc agtcggacct tgtctcctgc gtgaccacgt cgaccgcgcc catcgtcaag
     7921 ggcgaatggc tcaggcctgg aacccatgtg gatctcgccg gcgccttcaa gccctcgatg
     7981 cgcgagaccg atggcgaagc ggtggcacgg gccagtgtct atgtcgacac ccgcgacggc
     8041 gcactggcgg aggccggcgg tctgctccag gcccgcgacg aaggccgctt cgacttcgcc
     8101 agcgtgaagg gcgatctgtt cgacctgtgc cgcggtaagg cggccggccg ggcgtcatct
     8161 ggcgaaatca ccctgttcaa gtcctgcggc accgccatcg aagaccttgc caccgccatc
     8221 atggtgtatc aaaaggcggc atgaccgccg ccagccccct ctgcatcgcc atcgacacgc
     8281 cggatctgga ccgggcgaca agcctagcgc gcgagctgag gcccagcatc ggctacgtca
     8341 agctcggcat ggagttcttc tacgcgcatg gggctgcggg atatgcccgc gttgcagagt
     8401 ccggggtgcc catcttcctc gacctcaagc tgcatgacat tcccaatacc gtggcagagg
     8461 gcctgcgttc cctgctgcgg ctcgatccga ggccggccat cgtcaatgtc catgcgacgg
     8521 gcgggctcga catgatgaag gccgccgccg acgcggtggc cgggcaatgc aagctcatcg
     8581 cagtgacgat cctcaccagc ctctcggacg acgatatctg ggcggcaggc ttcgagaccg
     8641 cgcgcaatac cgagggccat gtccggtcgc tggcagccct ggccagatct g
//
LOCUS       DBNGVR010000365         2635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1314072, whole genome shotgun sequence.
ACCESSION   DBNGVR010000365 DBNGVR010000000
VERSION     DBNGVR010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1314072"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="AC16A0_07760"
     CDS             <1..689
                     /locus_tag="AC16A0_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3105268.1"
                     /translation="ISRLGKGASSTWILGKPVEADLKSALGRDIRVENDADCFAASEA
                     VDGAGAGYTVVFAVILGSGAGAGIAVNSRAHHGPNNSGGEWGHNPLPFPDTTEIPGKA
                     CYCGRHGCMETWVSGRAFEAQYTEHKGVELKAAEIMDLKRKGDHLANLLWQRYIDRVA
                     RGLATVVNTLDPDVLVMGGGMSNVDELYVDLPPALARRTFSTVFHTPILRNRHGDASG
                     VRGAAWLWRD"
     gene            692..1474
                     /locus_tag="AC16A0_07765"
     CDS             692..1474
                     /locus_tag="AC16A0_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3105269.1"
                     /translation="MSLMRRYDFGLSLIRDAGELALGYFNNRTSLSIQSKGPQDMASE
                     ADLNTELLIKSRLAGAFPEDAFLGEETGRTAFAKDQGIWVVDPIDGTQPFICGLSSWC
                     VSIAFVRGGALQFGMVYAPARDELFAGGAGHLATLNGQPIRGAPAASVKDGLIGAGYS
                     PKSGVDQFLPAFERFLRAGGMFYRDGSGALGLCYVAAGRLIGFFEPVIRSWDCLGAVA
                     VVQAAGLRTNDFLTGDGLFSGNPIIAGNDAIYAELSALAGYA"
     gene            1531..2538
                     /locus_tag="AC16A0_07770"
     CDS             1531..2538
                     /locus_tag="AC16A0_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3105270.1"
                     /translation="MTQDDLEAAKRSAASWYWWGIPTFFRCPWNEDPSQCDIALVGVP
                     HSSGNGSTERDQHLGPRAVRNVSGFYRRAHGVHNVAPFEQLRISDLGDVPLPEAMNNE
                     ACVRDIEAYFARLDAAGARPVSIGGDHSITGPILKAIAGPGRKLSGGRPVALIHFDAH
                     TDSYENMPHWLGAKRSAAHWAAYTVREDAVDATRSTQVGMRGHPSGLIHGGKVGGSSE
                     ALGYRVIKMDEFERLGVEATVAIIRERAGDGPCYITFDLDCLDPSVAPGVSNLEPGFG
                     GFSIREATRLLQGLKGLDIIGGDVVCLMPTKDSPNNITAQVAMVMMFEILTLIALRNA
                     A"
BASE COUNT          434 a          850 c          874 g          477 t
ORIGIN      
        1 ccatcagccg ccttggcaag ggggcgagct ccacctggat ccttggcaag ccggtcgagg
       61 ccgatctcaa gtccgctctg ggccgggaca ttcgcgtgga gaacgacgcc gactgttttg
      121 ccgcttcaga ggctgtggac ggggcggggg cagggtacac tgtggtcttc gccgtgatcc
      181 ttggatccgg agcaggggcg ggcattgcgg tgaacagccg ggcgcaccac gggccgaaca
      241 acagcggcgg cgagtggggt cacaatccgc tgccctttcc cgataccacc gaaatcccgg
      301 gcaaggcctg ttactgcggc cgccatggct gcatggaaac ctgggtgtcg ggccgtgcct
      361 tcgaggcaca atacaccgag cacaagggcg tggagctcaa ggccgccgaa atcatggacc
      421 tgaagcgcaa gggcgatcac ctcgccaatc tgctctggca gcgctacatc gaccgcgtgg
      481 cgcggggcct tgcgacggtc gtcaatacgc tcgaccccga cgttctcgtc atgggcggcg
      541 gcatgtcgaa tgtcgatgaa ctctacgtcg atttgccgcc tgcgctggcc cggcggacct
      601 tctcaacggt gttccacacg ccgatcctgc gcaacaggca tggcgatgcc agcggcgtgc
      661 gcggcgcggc ctggctgtgg agggactgag catgtcgctg atgcgccgct acgacttcgg
      721 gctctcgctg atccgcgatg caggcgaact ggcgctcggc tatttcaaca accgcaccag
      781 cctttcgatc cagagcaagg gtccgcagga catggccagc gaggctgacc tcaataccga
      841 gctgctgata aagtcgcggc ttgccggggc ctttcccgag gacgcgtttc tcggcgaaga
      901 aacggggcgc acggcctttg cgaaggacca gggcatctgg gtggtcgatc ccatcgacgg
      961 cacccagccc ttcatctgcg ggctgtcgag ctggtgcgtg tcgatcgcct tcgtcagggg
     1021 tggcgcgctg cagttcggca tggtctatgc gccggcgcgg gacgaattgt tcgccggcgg
     1081 ggccggccat ctagccaccc tcaacgggca gccgatccgc ggtgcccctg ccgcttccgt
     1141 caaggacggg ctgatcggcg caggctattc gccgaagagc ggcgtcgacc agtttctccc
     1201 ggccttcgag cggttcctgc gcgccggcgg catgttctac cgcgacggtt cgggtgcgct
     1261 gggtctctgc tatgtggcgg caggccggct catcggcttc ttcgaacccg tcataaggtc
     1321 atgggattgc ctcggtgccg ttgctgtggt gcaggccgca ggcctcagga ccaatgactt
     1381 tctcacggga gacggtctct tctccggaaa cccgatcatc gcggggaacg acgcgatcta
     1441 cgccgagttg tctgccctcg ccggatatgc ctgacgcaca ggaccgtgac gcttgttccg
     1501 gcgccggacg caatctatcg tcgatccatc atgacccaag atgatctcga agccgccaaa
     1561 cgctctgccg catcctggta ctggtggggc attcccacgt tcttccgctg cccctggaac
     1621 gaagacccgt cccagtgcga tattgccctg gtgggcgttc cgcacagcag cggcaacggc
     1681 tcgaccgagc gcgaccagca ccttgggccg cgtgccgtgc gcaatgtctc cggcttctat
     1741 cgccgggccc atggggttca caacgttgcg cccttcgagc agttgcgcat cagcgatctg
     1801 ggcgacgtgc cgttgccgga ggccatgaac aacgaggcct gcgttcgcga catcgaggcc
     1861 tatttcgcgc ggctggatgc agccggcgcg cggccggttt cgatcggcgg cgatcactcg
     1921 atcaccgggc cgatcctcaa ggccattgcc ggaccagggc gcaagctctc gggcggcagg
     1981 ccggtggcgc tgatccactt cgatgcccac actgacagtt atgagaacat gccgcattgg
     2041 ctgggcgcaa agcgttcggc ggctcactgg gccgcctaca cggtgcgcga ggacgcggtc
     2101 gatgccaccc gcagcacgca ggtcggcatg cgcggtcatc cctccggcct gatccacggc
     2161 gggaaggtgg gcggcagcag cgaggcgctg ggctatcgcg tcatcaagat ggacgagttc
     2221 gagcggcttg gcgtggaggc aaccgtcgcc ataatccgcg aacgcgcggg cgatggccct
     2281 tgctacatca ccttcgatct tgactgcctc gatccctccg tggcgcccgg cgtgtccaat
     2341 ctcgaaccgg gcttcggcgg cttctcgatc cgcgaggcga cgcgattgct gcaggggttg
     2401 aaggggctgg acatcatcgg tggagacgtc gtctgcctga tgccgaccaa ggacagccca
     2461 aacaacatca cggcgcaggt cgccatggtg atgatgttcg agatcctgac gctgatcgcc
     2521 ttgcggaacg ccgcctgatc aggcgggatg ccaccacttg ccggcgatga cgacgccgcg
     2581 gctgttgatc ttgcggtcgc cggtcaggtg ctcacccacg acatcgacgt agtcg
//
LOCUS       DBNGVR010000366         3181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3278806, whole genome shotgun sequence.
ACCESSION   DBNGVR010000366 DBNGVR010000000
VERSION     DBNGVR010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3278806"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1026
                     /gene="iolG"
                     /locus_tag="AC16A0_07775"
     CDS             37..1026
                     /gene="iolG"
                     /locus_tag="AC16A0_07775"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HIR3105271.1"
                     /translation="MIRFGVLGAGRIGKVHSRTIAASKQATVAYLADAMPEAAASLAA
                     EVGAKVASVEDIIKAKDVDAVLIATPTGTHADLIEAASNAGKAILCEKPVSLSVERIL
                     ACLKVVEKNKSNLMIGFNRRFDPNFAALAQRLHAGAIGNLELATIISRDPAPPPADYV
                     KGSGGLFRDMMIHDFDLARFLVGEEFVTVHALGSALVDKAIGAAGDVDTAAVHMQTAS
                     GKIAVITNSRRATYGYDQRIEVHGSKGMLRAGNVHNTTVELANADGFQGDPILNFFTE
                     RYGQAYANEVLAFIDGMAKGVSARPNGHDGLQAQKLADAATESWSSGKPVKVG"
     gene            complement(1036..1302)
                     /locus_tag="AC16A0_07780"
     CDS             complement(1036..1302)
                     /locus_tag="AC16A0_07780"
                     /inference="COORDINATES: protein motif:HMM:NF023159.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3105272.1"
                     /translation="MRLNTIPGIGKTFERDFARIGILDVAQLRGADPDALYASLVQAN
                     ADEGHRTSRNYLYVIRMAVYFADGGREVVKLKWSAWTDKATKRC"
     gene            1354..2187
                     /locus_tag="AC16A0_07785"
     CDS             1354..2187
                     /locus_tag="AC16A0_07785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3105273.1"
                     /translation="MTDGDTFKNLSMDGKIAVVTGSTQGLGEAICNLFADRGVKGLVI
                     CGRNAARGAKVKASLEAKGVKTVFVQADLGRLAEVRKVVSACDKEFGRIDALVNAAAL
                     TDRGSIWDTTPERYDEIFDVNVKAPFFLMQDALHLMKKSKSKGTIVNILSMSGHGGQD
                     FITAYCGSKGALAVLTRNVAFSVMRYHIRVNGLNIGWMDTPGEDRIMKTYHDAADGWL
                     KKAEAARPFGRLLKPVEVARAVGYLSSDESGLMTGSIIDFDQQVLGTGNAIPEPPAAS
                     W"
     gene            2198..>3181
                     /locus_tag="AC16A0_07790"
     CDS             2198..>3181
                     /locus_tag="AC16A0_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR3105274.1"
                     /translation="MNFASDNVYGVHSRIMEALTRVNDGTAASYAGDDLSKRAETMLS
                     DVFETEVRAFLVTTGTAANGLALSAITPGYGAILCHAEAHISCDECNSPEFFTGGAKI
                     LGLHGAGGKIAPALIEKTLKGFIRGEHDPKPRGVSITNATELGTVYRPSEVKAISDLI
                     RPRGMKLHMDGARFANAVAGLGVSPAEVTWKSGVDVMSFGATKNGAMMLEAVVFFDLA
                     LAEDFAYRRMRAGQLISKSRYLGAQMVAYLENGLWLENARRANALAQKLAAGLAQVNA
                     VRIPNPVEANEVFAIIPRKLNDRLLASGARFYDWMPDSLGNAIGADEIFVRF"
BASE COUNT          621 a         1008 c          996 g          556 t
ORIGIN      
        1 ccccctgcag ggccagccgt cacatgggag agcaagatga tcagatttgg agtcctgggc
       61 gccggacgga tcggcaaggt ccattcccgc accatcgccg catcgaaaca ggcaaccgtc
      121 gcctatctgg ccgacgccat gccggaggct gcagcaagcc ttgcagcgga ggtcggcgcc
      181 aaggtcgcat cggtcgagga catcatcaag gcgaaggacg tggacgcggt gctgattgcc
      241 acgccgaccg gcacccatgc tgacctgatc gaagcggcct ccaatgccgg caaggccatt
      301 ctctgcgaaa agccggtgag cctctcggtc gagcgcatcc tcgcctgcct caaggtggtc
      361 gagaagaaca agtcgaacct gatgatcggc ttcaaccgcc ggttcgaccc caatttcgcg
      421 gcgctcgccc agcggctcca cgccggcgcc atcggcaatc tcgaactggc gaccatcata
      481 agccgcgatc cggcgccgcc gccggccgac tatgtgaagg gctcgggcgg attgttccgc
      541 gacatgatga tccacgactt cgatcttgcg cgcttcctgg tgggcgagga attcgtgacc
      601 gttcacgcgc tgggttccgc gcttgtcgac aaggccatcg gcgccgcggg cgacgtcgat
      661 accgccgcgg tacacatgca gaccgcatcc ggcaagatcg ccgttatcac caattcgcgc
      721 cgtgcgacct acggatatga ccagcgtatc gaggtgcatg gctcgaaggg catgctgcgt
      781 gccggcaacg tgcacaacac gacggtcgaa cttgcaaatg ccgacggttt ccagggtgac
      841 cccattctca actttttcac agagcgctat ggccaggcct atgccaacga ggtgctggcc
      901 ttcatcgacg gcatggccaa gggcgtgtcg gcgcgaccca acggtcatga cggacttcag
      961 gcgcagaagc tggccgatgc cgcaaccgag tcctggtcaa gcggcaagcc cgtgaaggtc
     1021 ggctgagccc ggaactcagc agcgttttgt ggccttgtca gtccaggcag accactttag
     1081 cttgaccacc tctcgtccgc cgtccgcgaa atagacagcc atgcggatca cataaaggta
     1141 attcctgctt gtcctgtggc cctcatcagc attggcctgc accagactcg cgtagagggc
     1201 gtcgggatcg gcgccgcgca gctgcgcgac gtcgagaatg ccgatgcgtg caaagtcacg
     1261 ttcgaaggtc ttgccgatgc ctggaatggt attcagacgc atgccactga tacccgacaa
     1321 tcaagatctc ggatcaccga aggggaatga ctcatgacag atggcgacac attcaagaac
     1381 ctctccatgg acggcaagat cgccgtcgtc accggatcga cccaagggct tggcgaagcc
     1441 atctgcaacc tcttcgccga tcgtggcgtg aaggggctgg tgatctgcgg ccgcaatgcg
     1501 gcgcgcgggg cgaaggtgaa ggccagtctc gaggccaagg gcgtgaagac ggtcttcgtg
     1561 caggccgacc ttggccggtt ggccgaagtg cgaaaggtcg tctccgcatg cgacaaggag
     1621 ttcggccgca tcgacgcact ggtcaatgcc gcggccctta ccgaccgcgg ctcgatctgg
     1681 gacacgaccc ccgagcgata tgacgagatc ttcgatgtca acgtgaaggc gcccttcttt
     1741 ctgatgcagg atgcgctgca cctgatgaag aagtcgaaga gcaagggcac catcgtcaac
     1801 atcctgtcca tgtctggcca tggcgggcag gatttcatca ccgcctattg cggctcgaag
     1861 ggcgcgcttg cggtcctgac acgaaacgtg gccttcagcg tcatgcgcta ccacatccgc
     1921 gtcaatggct tgaacatcgg ctggatggat acgccgggcg aggatcgcat catgaagacc
     1981 tatcatgacg ccgccgacgg ctggctcaag aaagccgaag ccgcgcggcc cttcggacgc
     2041 ctgctcaaac ccgtcgaggt ggcgcgggcg gtgggctatc tgtcgagcga tgaatcgggg
     2101 ctgatgaccg ggtccatcat cgacttcgac cagcaggtgc tgggcaccgg caatgccata
     2161 cctgaacccc cggcggcgag ttggtgagcg cggccacatg aatttcgcat ccgacaatgt
     2221 ctatggcgtc cactcccgca tcatggaggc cctcacccgc gtcaacgacg gcacggccgc
     2281 ctcctatgcc ggcgacgatc tgtcgaaacg cgcggagacc atgctctcgg acgtcttcga
     2341 gacggaggtc cgcgccttcc tggtgaccac cggcacggcg gcaaacgggc tggcgctctc
     2401 cgccatcacc cccggttatg gcgccatcct gtgtcacgcc gaggcgcata tctcctgcga
     2461 cgaatgcaat tcgccggagt tcttcaccgg cggcgccaag atcctgggcc tgcatggcgc
     2521 gggcggcaag atcgcgccgg cgctgatcga aaagacgctg aaggggttca tccgcggcga
     2581 gcatgacccc aagccgaggg gcgtttcgat caccaatgcc accgagcttg gaacggtcta
     2641 caggccttcg gaggtaaagg cgatctccga cctcatccgc ccgcgcggca tgaagctgca
     2701 catggacggc gcgcgctttg ccaatgccgt ggcgggactt ggagtatcgc ccgccgaggt
     2761 gacatggaaa tcgggcgtcg acgtcatgtc cttcggcgcc accaagaacg gcgccatgat
     2821 gctggaggcc gtggtgttct tcgatctggc tttggccgag gacttcgcct atcgccgcat
     2881 gcgcgccggt cagctcatct cgaagagccg ctatctcggc gcccagatgg tggcctatct
     2941 ggagaacggc ctgtggctgg aaaacgcacg gcgcgccaac gcgctggcgc agaaactggc
     3001 ggcgggcctc gcgcaggtga atgcggtgcg catccccaat ccggtcgagg ccaacgaggt
     3061 cttcgccatc attccccgca agctgaacga caggctgctg gcgagcggcg cgagattcta
     3121 tgactggatg cccgacagcc tcggcaatgc catcggggca gatgagatct tcgttcgctt
     3181 c
//
LOCUS       DBNGVR010000367         6570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1904974, whole genome shotgun sequence.
ACCESSION   DBNGVR010000367 DBNGVR010000000
VERSION     DBNGVR010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1904974"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="AC16A0_07795"
     CDS             complement(<1..1345)
                     /locus_tag="AC16A0_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105275.1"
                     /translation="MTPSLWLRFALRDLRSGLQGFWIFLTCLALGTAAIAIIGSLSAA
                     IERGLDEQGQPLLGGDVEFSLIHREASPDELAYITSKGEVSKVATVRAMAVAGEATTL
                     VEAKAIDGAYPLYGSLTLENGAALDGIFDKRGGVYGAVADPLLTGRLGIKPGDRIKLG
                     SIEIAIRGLIATEPDRISDGIVLGPRLLLSEEALRATGIVQPGSLITWRYRVKLADPS
                     LAAANALIEESKETFPDAGWRVRNRNQAASGADRFIERLGYFMTLVGLASLIVGGAGI
                     ANAVAAFVNRRSNSIATLKCLGASSRTVFGIYLTEITLVALIAIAIGLAAGAVAPMLA
                     YGLLSAIIPLPIAARVEWLPLAIAGALGLLVTYAFAIWPLAHTRRVPASALFRHRILA
                     IHGWPSFAELLAIAAALAGIGLIVFFSFEERRITGYFLGGLVASFVLLLGLARLII"
     gene            complement(1342..2028)
                     /locus_tag="AC16A0_07800"
     CDS             complement(1342..2028)
                     /locus_tag="AC16A0_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105276.1"
                     /translation="MTDIDAVSLRDVHLTLPSRAGNVDILRGVSLTARAGEALGIVGP
                     SGSGKTTLLMVVAGLEQANAGAVHVAGESLTGRSEDDLAVFRRDHVGIVFQSFHLIPT
                     MTALENVAVPLEFRGSPDAMSIAADRLERVGLSHRMTHYPGQLSGGEQQRVAIARALA
                     SGARIILADEPTGNLDHDTGEQIIKLLFDLRTQEGATLLLVTHDPDLARRCDRVAEVR
                     DGKIVSGAHP"
     gene            2112..2735
                     /locus_tag="AC16A0_07805"
     CDS             2112..2735
                     /locus_tag="AC16A0_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR3105277.1"
                     /translation="MSKIIAAFAAVFMLTQAASAQTITILALGDSLTAGYGLQPSDAF
                     PVKLEQALRAKGHDVVVVNAGVSGDTALDGLSRLDWALSEEVDAAIVEFGANDALRGL
                     QPAQAEAAIDGILGKLGEKKIPTLLAGMRAPPNLGPEYMSAFDGMYARLAGKHEALFY
                     PFFLDGVAADIKLNQADGMHPNAAGVDVIVARILPFVEELVTKAAAK"
     gene            2813..3355
                     /gene="thpR"
                     /locus_tag="AC16A0_07810"
     CDS             2813..3355
                     /gene="thpR"
                     /locus_tag="AC16A0_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR3105278.1"
                     /translation="MPRLFTGLELPDDVATDLGLMKGGIMGARWIDRESFHLTLRFIG
                     DIDDSLAREIAYALDGVAARPFSLRLRGMGLFGGNKPHTLYAGVEESPELRRLQSIHE
                     RICQVLGLAAEGRKFMPHVTLARLKDPDPLALHGYVASHNLYKSRTFEVSRFVLFSSR
                     PSRGGGPYAVEESYQLQGSP"
     gene            3352..3654
                     /locus_tag="AC16A0_07815"
     CDS             3352..3654
                     /locus_tag="AC16A0_07815"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3105279.1"
                     /translation="MTETPLHGVERVRVLRELQGWTEAKGRDAISKTFQFKSFNQAWG
                     WMSRVALFAEKTDHHPEWFNVYGRVEVTLSTHSAGGVTEKDIELARKMDGYAAVLA"
     gene            3742..4467
                     /locus_tag="AC16A0_07820"
     CDS             3742..4467
                     /locus_tag="AC16A0_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR3105280.1"
                     /translation="MAEVRYQPQTTAQSRAEIDQGLRSYMMGVYNYMALGVAATAIIT
                     MAVAANPAAMQAVYSLKWLFFIAILGMGFLAPRLIFSANPAVAHGAYWGYVALWGLAI
                     APMVGTYLAIGPDIVYRAFLITAVTFGATSLAGYVTKRDLSGLGAFFMMASIGIIIAM
                     LVNVFFFQSTMMSLITSIVTVLLFAGITAYETQQIKEMYVEGDTPGVARGKSIFGAFM
                     LYGSFITLFIHILNILGIMRSSE"
     gene            complement(4538..4933)
                     /locus_tag="AC16A0_07825"
     CDS             complement(4538..4933)
                     /locus_tag="AC16A0_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HIR3105281.1"
                     /translation="MDEQEPITSLRTWGASTRSGPEAPRETGARRPEQIAFDRKELTL
                     ILGLYGRKVADGEWRDYAMEFGRDKAIFSVFRRSSEMPLYRIVKDPSLARRQGLYSVV
                     AQGGLILKRGADLTQVLKVLIKAPKLTAV"
     gene            5145..5744
                     /locus_tag="AC16A0_07830"
     CDS             5145..5744
                     /locus_tag="AC16A0_07830"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3105282.1"
                     /translation="MIPSIGSAAASTAEERRILRFDNKTTTRKASTSSKKKKKIVRKA
                     PAYKGRALVDFDTPEKPGTIIIRTNERALYRVMEDGKAMRYLVAVGKDGFNWSGVATV
                     GMKRKNPTWTPPAEMIKRTPKYAKWRNGMPGGIPQNPLGARALYLFKGGRDTMFRIHG
                     TNAPSSIGTAASSGCIRMLNHEVIELYNKTPVGTKVIVL"
     gene            5816..6319
                     /locus_tag="AC16A0_07835"
     CDS             5816..6319
                     /locus_tag="AC16A0_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HIR3105283.1"
                     /translation="MSLTDLAWLSLAAYAVHMLEEFMLNWRDWARGVIRLPVTWPDFY
                     MTNGIVVVLGIASASLAPTLPLAPMIFAALMLINAVFFHIVPVVVTRGRYSPGVATAV
                     MLFLPLGYAVLHKARVDGHAAWGTLLSAFLIGAALMAYPIILLHLRGLRYFQQAEAAD
                     AGVAPKT"
     gene            complement(6335..>6570)
                     /locus_tag="AC16A0_07840"
     CDS             complement(6335..>6570)
                     /locus_tag="AC16A0_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HIR3105284.1"
                     /translation="LSQHATANAFDIAGFRLENGRTVSLKSGWDAGGATSEFLRDLRG
                     RACLLFNMVLSPDYNADHADHFHVDMGPYMGCN"
BASE COUNT         1180 a         2097 c         2079 g         1214 t
ORIGIN      
        1 cgatgatgag ccgggcgagg cccagcagca gcacgaagct ggccaccagg ccgccgagga
       61 aatagccggt gatgcgccgc tcctcgaagc tgaagaagac gatcagtccg atgccggcga
      121 gggcggcggc gatggccagc agttcggcga aactgggcca gccatggatg gccaggatgc
      181 ggtggcggaa cagggccgag gccggcacgc ggcgcgtatg cgccagaggc cagatggcga
      241 aggcataggt caccagcagc ccaagcgcgc cggcgatcgc cagcggcagc cattcgacgc
      301 gcgccgcaat gggcagcgga atgatggcgc tcagcaggcc ataggcaagc atgggcgcca
      361 ccgccccggc ggccagcccg atggcgatgg cgatcagcgc caccagcgtg atctcggtga
      421 ggtagatgcc gaagacggtg cgcgacgatg cgccgaggca tttcagcgtt gcgatggaat
      481 tgctgcggcg gttgacgaag gcggccaccg cattggcaat gccggcgccg ccgacaatca
      541 gcgacgcaag tccgaccagc gtcatgaagt agcccagccg ctcgatgaag cggtcggcgc
      601 cggaggctgc ctgattgcgg ttgcgcaccc gccagccggc gtccggaaac gtctccttcg
      661 actcctcgat cagcgcgttg gcggccgcga gactgggatc ggccagcttc acgcggtagc
      721 gccaggtgat caggctgccg ggctgcacga tgccggtggc gcgcagcgcc tcttcgctca
      781 gcagcaggcg ggggccgagc acgatgccat cggagatgcg gtccggctcg gtggcgatga
      841 ggccgcggat ggcgatctcg atgctgccca gcttgatgcg gtcgcctggc ttgatgccga
      901 ggcggccggt gagcagcgga tcggccacgg cgccatagac gccgccgcgc ttgtcgaaga
      961 tgccatcgag agccgcaccg ttctccagcg tcagcgatcc gtagagcgga taggcgccgt
     1021 cgatggcctt ggcctcgacc agcgtggtgg cctcgccagc gaccgccatg gcccggaccg
     1081 tcgccacctt gctgacctcg cccttggagg tgatgtaggc gagttcgtcg ggcgatgcct
     1141 cgcggtggat cagcgagaac tcgacgtcgc cgcccagcag cggctggccc tgctcatcga
     1201 gcccgcgttc gatggcagcc gagagcgagc cgatgatggc gatggcggcg gtgcccagcg
     1261 ccaggcaggt gagaaagatc cagaagccct ggagacccga tcgcaagtca cgcagggcaa
     1321 agcgcagcca cagcgatgga gtcacggatg cgccccggaa acgatcttgc cgtcacgcac
     1381 ttcggccacg cggtcgcagc ggcgggcgag gtcgggatca tgggtcacca gcagcagcgt
     1441 ggcgccctcc tgtgtcctga gatcgaagag cagcttgatg atctgctcgc cggtgtcgtg
     1501 gtcgaggttg ccggtgggct cgtcggccag gatgattcgc gcgccggacg cgagggcgcg
     1561 ggcgatggcc acgcgctgct gttcgccgcc cgagagctgg cccggatagt gcgtcatgcg
     1621 atgggaaagg ccgacccgct caagccgatc cgcggcaatg gacattgcat ccggactgcc
     1681 gcgaaactcg agcggaacgg ccacgttctc cagcgccgtc atggtcggta tgagatgaaa
     1741 ggactggaac acgatgccca catgatcgcg ccggaacacg gcaagatcgt cctcgctgcg
     1801 gccggtcagg gattcgcctg cgacatgaac cgctccggca ttggcctgtt ccagcccggc
     1861 cacgaccatg agaagcgttg tcttgccgga gcccgatggc ccgacaatcc ccagcgcttc
     1921 gccggccctg gccgtcaggc tcacgccgcg caggatgtcg acattgccgg cgcggctcgg
     1981 cagggtcagg tgaacgtccc ggagggacac ggcgtctatg tctgtcatcg tgtcgaacat
     2041 gggtgtcgat acgtgatttg tcgagccacg gatgcttgaa ggcggtacaa tcgcgcccta
     2101 ccttcaaggc catgtcgaaa atcatcgccg cgtttgctgc cgtcttcatg ttaacccagg
     2161 ctgcatcagc gcagaccatc accatccttg cgctgggaga tagtctgacg gccggctatg
     2221 gactgcagcc atccgatgcc ttcccggtca agctcgaaca ggccctgagg gcgaaggggc
     2281 atgacgtcgt ggtcgtcaat gccggggttt cgggcgacac ggcactggac ggcctgtcgc
     2341 ggctggactg ggccctgtcg gaggaggtgg atgcggcgat cgtcgagttc ggcgccaatg
     2401 atgccttgcg cggcctgcag cccgcccagg cggaagccgc catcgacggc atcctcggca
     2461 agttgggcga gaagaagatc cccacccttc ttgccggcat gcgggcgccc cccaatctcg
     2521 gcccggagta catgtcggcc tttgatggca tgtatgcgcg gcttgccggg aagcacgagg
     2581 cccttttcta tcccttcttc ctcgatggcg tggcggcgga catcaagctc aaccaggccg
     2641 acggcatgca tcccaacgca gccggcgtcg atgtgattgt ggcgcgaatc ctgccctttg
     2701 ttgaagaact tgtcacgaag gctgcggcaa aatagggctt ggccttcggc cgaatcacgt
     2761 tagcttaacg gttagcgacc cgtgcagacc ccccctcgaa aggaggcccg ccatgccgcg
     2821 actcttcacc ggccttgaat tgccggacga cgtcgccacc gatctcggcc tgatgaaggg
     2881 cgggatcatg ggagcccgct ggatcgaccg ggaatccttt cacctcaccc tccgcttcat
     2941 cggcgacatc gacgattcgc tggcccgcga aatcgcctat gcgctggacg gggtggcagc
     3001 gcggcctttc tcgctccgac tgcgcggcat gggcctgttt ggcggcaaca agcctcacac
     3061 gctctatgct ggagtcgaag aaagccccga actgcggcgg ctgcaatcca tccacgaacg
     3121 catctgccag gtcctgggcc ttgccgccga ggggcgcaag ttcatgccgc atgtgacgct
     3181 ggcccggctc aaggacccgg atccccttgc gctccatggc tatgtcgcct cgcataatct
     3241 ctacaagagc cggactttcg aagtgtcgcg tttcgtgctc ttctcctccc ggccgtcacg
     3301 gggcggtgga ccctatgcgg tcgaggaatc ctatcaattg caaggctcac catgactgaa
     3361 acaccgttgc acggcgtcga gcgggtccgg gtcctccgcg aactccaggg ctggaccgag
     3421 gcgaagggac gggatgccat ttcgaaaacc ttccagttca agtcgttcaa ccaggcctgg
     3481 ggctggatga gccgggtggc gctgtttgcc gagaaaaccg accatcatcc cgaatggttc
     3541 aatgtttacg gccgggtgga ggtgacgctg tcgacccatt cggctggcgg ggtcaccgaa
     3601 aaggacatcg agctggcccg caagatggac ggctacgcgg ccgttcttgc ttaattacaa
     3661 tctcttaatg tgcctgaccg cttgaagctg gtacttcatt accccatatt gcagcagaat
     3721 tcttcacctg gagggagcga aatggctgaa gtccgatacc aaccccaaac aactgcccag
     3781 tcgcgggccg agatcgatca gggtctccgt tcgtacatga tgggcgtcta caactacatg
     3841 gcgcttggcg tggctgccac ggcgatcatc accatggcgg tggccgcaaa cccggctgcc
     3901 atgcaggctg tctattcgct gaagtggctg ttcttcattg ccatcctcgg catgggtttc
     3961 ctcgcaccgc gcctgatttt ctctgccaac ccggcggtcg cccatggcgc ctactggggc
     4021 tacgtggcgc tctggggcct tgccatcgcg ccgatggtcg ggacctatct cgccatcggg
     4081 ccggatatcg tctaccgcgc cttcctgatc accgccgtca ctttcggcgc caccagcctt
     4141 gcgggctacg tcaccaagcg tgacctgtcg ggcctcggtg ccttcttcat gatggcctcg
     4201 atcggcatca tcatcgccat gctggtgaac gtgttcttct tccagagcac catgatgagc
     4261 ctcatcacct cgatcgtcac cgtgctgctc ttcgccggca tcaccgccta cgagacccag
     4321 cagatcaagg agatgtatgt cgaaggcgat acccccggcg ttgcccgcgg caagtcgatc
     4381 ttcggcgcct tcatgctcta cggcagcttc atcacgctgt tcattcacat cctgaacatc
     4441 ctcgggatca tgcgcagctc ggagtgagag aacgacagca ccaaaagcag aggcccggcg
     4501 gaaacgccgg gccttttcca tttttgccgg agttggttca gaccgcggtg agcttcggag
     4561 ccttgatcag gaccttcagg acctgggtga gatcggcgcc tctcttgagg atgagccccc
     4621 cctgtgccac gaccgagtaa aggccttggc gccgggccag cgacgggtcc ttgacgatcc
     4681 ggtagagcgg catctcgctg ctgcggcgga agaccgagaa gatcgccttg tcgcggccga
     4741 actccatggc gtagtcgcgc cattcgccat ccgcgacctt gcggccatag aggccgagaa
     4801 taagggtaag ctccttgcgg tcgaaggcaa tctgctccgg ccgccgtgca cctgtctccc
     4861 ggggtgcttc cgggccggac cttgtgcttg cgccccaggt ccggagggaa gttatcggct
     4921 cttgttcgtc caaacgcccc accctgtgct gacggggttc atgttcctgt catgatgtac
     4981 ccaaagcggc gccctgacaa gcgtcagcgc gcgctactca cagcgctgtg acttgaaatc
     5041 cattccatca caatgtagtt ggttctgggg cctttccggg gtcgggggat atcttgcaac
     5101 gacgtggttt tcttggactt ttcggtgtgg cgctggtggc gtccgtgatt ccttcaatcg
     5161 gtagcgcggc agcctccacg gccgaagagc gtcgaatcct ccgcttcgac aacaagacga
     5221 ccaccaggaa ggcttccacc agcagcaaga aaaagaagaa gatcgttcgc aaggcgccag
     5281 cctacaaggg ccgtgcgctg gtggatttcg acacgcctga aaagccagga accatcatca
     5341 tccgcaccaa tgaacgggct ttgtaccgcg tcatggaaga cggcaaggcc atgcgttatc
     5401 tggtggccgt cggcaaggat ggcttcaact ggtcgggcgt cgccacggtc ggcatgaagc
     5461 gcaagaatcc cacctggacg ccgccggccg aaatgatcaa gcgtacgccc aaatacgcca
     5521 agtggcggaa cggcatgccc ggcggcattc cgcagaaccc gctgggtgcc cgcgccctct
     5581 acctcttcaa ggggggccgt gacacgatgt ttcgcatcca tggtaccaat gcgccgtcct
     5641 cgatcggcac cgccgcctct tcgggctgca tccgcatgct caaccacgag gtgatcgaac
     5701 tctacaacaa gacgccggtc ggcacgaagg tcatcgtcct gtaaggtctt cagcggtcga
     5761 ctacgactga cgaccatgag ataacccgga caccgaatat ctggggatgt ctgtcatgtc
     5821 gctcacggat ctcgcatggc tgtcgctggc cgcctacgcc gttcacatgc ttgaggagtt
     5881 catgctcaat tggcgcgatt gggcacgcgg agtgatccgc ctcccggtga cctggccgga
     5941 cttctacatg accaacggca tcgtcgtggt ccttggcatt gccagcgcgt ccctcgcgcc
     6001 cacacttccg ctcgcgccca tgatcttcgc cgcgctgatg ctgatcaacg ccgtgttctt
     6061 tcacatcgtg ccggtcgtgg tcacgcgtgg ccgctactcg ccgggtgttg ccacagccgt
     6121 catgcttttc ctgccgttag gctatgccgt gctccacaag gcccgtgtcg atggtcatgc
     6181 ggcatggggc actctgcttt cggccttcct gatcggtgcg gccctcatgg cctatcccat
     6241 catcctgctg catcttcgcg gcctgcgtta cttccagcag gccgaggccg ccgatgcggg
     6301 cgtcgccccg aagacctaga ataccggaac gcctctagtt gcagcccatg taggggccca
     6361 tgtcgacgtg gaaatggtcg gcatggtcgg cattgtagtc cgggctcagc accatgttga
     6421 agagcaggca ggcccgcccg cgcaggtcgc ggagaaattc cgaggtggcg ccaccggcat
     6481 cccatcccga cttgagcgag actgtcctgc cattctcgag ccggaagccc gcgatgtcga
     6541 atgcattggc cgtggcatgc tggctcagcc
//
LOCUS       DBNGVR010000368         3636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2493602, whole genome shotgun sequence.
ACCESSION   DBNGVR010000368 DBNGVR010000000
VERSION     DBNGVR010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2493602"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16A0_07845"
     CDS             complement(<1..356)
                     /locus_tag="AC16A0_07845"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105285.1"
                     /translation="MMFATAPAMAADHPSIAYMKQFAKEMLNAHRQGTVGSFRKVIER
                     HADVAAIADYSLGQYRKKLPAGQKQRYYGGVVTFMSRYFADQSRIYPIAKYEIGDAAA
                     DDGEVVINSKVYRLSG"
     gene            complement(488..1102)
                     /locus_tag="AC16A0_07850"
     CDS             complement(488..1102)
                     /locus_tag="AC16A0_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3105286.1"
                     /translation="MTGFVMRPLRRRALLGLATAAIFAPVLPTAAAIDPAEAYVRKVA
                     DQVMSLANSGQKGNGLKARFAALLNSHINLRAIANFALGTYQSKLPASRKGEFYELVN
                     NYAASLFVYYVDDFRGSELEVMSNAKQGKFTVIQSAIKLKGGGREQVRWRLTKSGGGY
                     RISDVNLKGVWLTISMKDRFTKVLNKSKGNFEPLFAELREAETW"
     gene            1290..2288
                     /locus_tag="AC16A0_07855"
     CDS             1290..2288
                     /locus_tag="AC16A0_07855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3105287.1"
                     /translation="MDELLAGLRAAAESTRIRILFVLSHGEFNVSELTQILGQSQPRV
                     SRHLKLMTEAGLITRHKEGNWVLFRLREGDLGGALSRAIVDLLPGQDAELARDLARLE
                     EVRATRAAAAARYFSDNAAQWETLRRLHVREEDVEAAMLNLAGPQPIALHADLGTGTG
                     SVLKSFARLASQSIGIDSSREMLAVARAQLDQAGIRHAQVRHGDIYALPFSDASADFV
                     TIHQVLHFLDDPARAVAEAARILKPGGRILIADFAPHDMEQLREHHAHRRLGIGTDQM
                     MQWLQRASLVLARHDSLAPPWRKEGTGLSVSLWLAGKPAIPSSHRTTTYRTSEARP"
     gene            2285..3169
                     /gene="metF"
                     /locus_tag="AC16A0_07860"
     CDS             2285..3169
                     /gene="metF"
                     /locus_tag="AC16A0_07860"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR3105288.1"
                     /translation="MMTTTDATGGLSVSFEFFPPKAGEAETTFWRTIAKLEPVHPSFV
                     SVTYGAGGTTRDRTLATVARIARETSLKPAAHLTCVGASKDEMNAVIREFRDAGVNHI
                     VALRGDPPGGVGTRFVPHPDGYRNAADLVRGIREIGDFEVSVSAYPERHPESVSIEED
                     LAFMADKADHGATRALSQFFFHNAFFLRLRDRIAARGIGIELVPGILPITSFARVQDF
                     AAKCGAHIPPALARRFEGLDEDPETRNLVAAMVAAEQVSALQREGVNHFHFYTLNRAD
                     LVYAICRVLGLGSEAKAA"
     gene            complement(3166..>3636)
                     /locus_tag="AC16A0_07865"
     CDS             complement(3166..>3636)
                     /locus_tag="AC16A0_07865"
                     /inference="COORDINATES: protein motif:HMM:NF023354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HIR3105289.1"
                     /translation="MHFAHDPVPGLNEQLGPRIDRVLANVQVGTPVVRFNWFLTPISA
                     RLFPDGPHDANVALARKVAASLADDHRRCGDRLWIRSERQTFMRLPQTMALAFGIHTY
                     SDPLSSLADDRESLAALHKLLGDYTDERLSYSAMLDLRAPVLRWIEDRLAVTDG"
BASE COUNT          654 a         1217 c         1144 g          621 t
ORIGIN      
        1 tgccccgaga gccgatagac cttgctgttg atcaccacct cgccgtcatc ggccgcggca
       61 tcgccgatct cgtacttggc gatgggatag atgcgcgact ggtcggcgaa gtagcgcgac
      121 atgaaggtga cgacgccgcc gtagtacctc tgcttctgcc cggccggcag cttcttgcgg
      181 tactggccga gcgagtaatc ggcaatggcc gccacatccg cgtgccgctc gatcaccttg
      241 cgaaagctac cgacggtgcc ctggcgatga gcgttgagca tctccttggc gaactgcttc
      301 atgtaggcga tggacggatg atcggccgcc atggccggtg cggtggcaaa catcatggcg
      361 gcgcccaaca cagcgcggcg gctcagcccc cgcctatgtt ctggtgtatg acgcctcatg
      421 attcggcacc cttcgagcca acgttccgca agctataatt gacgccttct gcgggcgtca
      481 actgaactta ccacgtctcg gcttcacgaa gttccgcgaa gagcggttcg aagttaccct
      541 tcgacttgtt caggaccttg gtaaagcgat ccttcatcga gatcgtcagc cagacgccct
      601 tcaggttcac gtcgctgatc cggtagccgc cgccggactt tgtcaggcgc cagcgcacct
      661 gctcgcgccc gccgcccttg agcttgatgg cgctctggat cacggtgaac ttgccctgct
      721 tcgcgttcga catgacctca agctccgacc cgcggaaatc gtcgacgtag taaacgaaga
      781 gcgaagcggc gtaattgttg accagctcgt agaattcgcc cttgcggctg gcgggcagct
      841 tgctctgata ggtgcccagc gcgaaattgg cgatcgcccg caggttgatg tgactgttga
      901 gcagcgcggc aaagcgcgcc ttgagcccgt tgcccttctg cccgctattg gcgagtgaca
      961 tgacctggtc ggcaaccttg cggacatagg cttctgcggg atcgattgcc gccgccgtcg
     1021 gcaggacagg cgcgaaaatg gccgcagtgg ccagccccaa tagtgcccgg cggcgcagcg
     1081 gacgcatcac aaaaccggtc attgctcctc ccaagcaccc tggactctcg ccgactataa
     1141 cgccgtgaaa ctatgaagaa agcaaggcga tcgaaagttc caacaggagt tttcagaacg
     1201 gtgccggcag gcttccggac cacatcgcag cggacaaggc ggttgaatta aacataaaga
     1261 tatgtttatg tccttataag gggaatgcaa tggatgaact tttggcagga ttgcgggcgg
     1321 cggccgagtc tactcggatt cgcattctct ttgtcctttc gcatggcgaa tttaacgtca
     1381 gcgagctgac ccagatcctg ggccagagcc agccgcgcgt cagccggcac ctgaagctga
     1441 tgacggaagc cggcctgatc acccgccaca aggaaggcaa ctgggtgctc ttccggctgc
     1501 gggagggcga cctgggcggc gccctgtcgc gcgccatcgt cgacctgctg cccggccagg
     1561 acgccgaact ggcccgcgac ctggcgcggc tggaagaggt ccgcgccacc cgtgccgccg
     1621 cggcggcacg ctacttcagc gacaatgccg cacaatggga aacgctgcgg cggctgcatg
     1681 tgcgggagga agacgtggaa gcggcgatgc tgaacctggc cgggccgcag cccatcgcgc
     1741 tccatgccga tctcggcacc ggcaccggct cggtgctcaa gtccttcgcc aggctcgcca
     1801 gccagtcgat cggcatcgac agcagccgcg agatgctcgc ggtggcccgg gcccaactcg
     1861 accaggcggg gatccgtcat gcccaggtgc gccacggcga catctatgcc cttccctttt
     1921 ccgacgcgtc ggcggatttc gtcaccattc accaggtgct gcacttcctc gacgacccgg
     1981 cccgcgccgt ggcggaagcc gcgcgcattc tcaagcccgg cggccgcatc ctcatcgcgg
     2041 atttcgcgcc gcacgacatg gaacagctcc gcgagcatca cgcccaccgc cgcctcggca
     2101 tcggtaccga ccagatgatg cagtggctgc agcgcgccag cctggtcctg gcccggcatg
     2161 acagcctggc gccgccctgg cgcaaggagg gcacgggcct cagcgtgtcg ctgtggctgg
     2221 ccggcaagcc cgccatacca tcgtcacacc gcaccaccac ctaccgcacc agcgaggcga
     2281 ggccatgatg accaccaccg atgcgacagg cggcctgtcc gtctccttcg agttcttccc
     2341 gcccaaagcc ggcgaggccg agaccacctt ctggcgcacc atcgcgaaac tcgagccggt
     2401 gcatccctcc ttcgtctcgg tcacctatgg cgcgggcggt accacccgcg accgcacgct
     2461 ggcgaccgtg gcacgaatcg cgcgggaaac gagcctcaaa ccggcggccc acctcacctg
     2521 cgtcggcgcc agcaaggacg agatgaacgc ggtgatccgc gagttccgcg atgccggcgt
     2581 caaccacatc gtggcgctca ggggcgatcc gccgggcggc gtcggcaccc gcttcgtgcc
     2641 acatccggac ggctatcgca atgccgccga cctggtgcgc ggcatccgcg agatcggcga
     2701 cttcgaggtg tcggtcagcg cctatccgga gcgccatccg gagtcggttt cgatcgagga
     2761 agacctggcc ttcatggccg acaaggccga ccatggcgcg acgcgcgcgc tctcgcagtt
     2821 cttcttccac aatgcattct tccttcgcct gcgcgaccgc atcgcggcgc gcggcatcgg
     2881 catcgagctg gtgcccggca tcctgccgat cacctccttt gcccgggtgc aggactttgc
     2941 cgcaaaatgc ggcgcgcata tcccgcccgc gcttgcccgc cgcttcgagg ggctcgacga
     3001 ggatcccgag acgcgcaatc tcgtcgccgc catggtggcg gccgagcagg tcagcgccct
     3061 gcagcgcgag ggcgtgaacc acttccactt ctacacgctg aaccgcgccg acctggtcta
     3121 tgcgatctgc cgcgtgctgg gcctgggcag cgaggccaag gcagcttagc cgtccgtgac
     3181 cgccagccgg tcttcgatcc agcgaagcac gggggcacgc agatcgagca tggcggaata
     3241 tgaaagtcgc tcatccgtgt agtcgccaag cagcttgtgg agtgccgcga gactctcgcg
     3301 atcgtcggcc agcgacgaga gcggatcgga ataggtgtgg atgccgaagg cgagtgccat
     3361 ggtctgcggc aggcgcatga aagtctggcg ctcggacctg atccagaggc gatcgccgca
     3421 gcggcggtga tcgtcagcca ggctcgcggc caccttccgc gccaatgcga catttgcgtc
     3481 gtgcggccca tccggaaaga ggcgggcgga aatgggcgtg aggaaccagt tgaagcgtac
     3541 caccggggtg ccgacctgca cgttcgccaa cacgcggtcg atgcgcgggc caagctgttc
     3601 attgaggccc ggcacgggat catgggcaaa atgcat
//
LOCUS       DBNGVR010000369         3439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2690222, whole genome shotgun sequence.
ACCESSION   DBNGVR010000369 DBNGVR010000000
VERSION     DBNGVR010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2690222"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="AC16A0_07870"
     CDS             <1..727
                     /locus_tag="AC16A0_07870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3105290.1"
                     /translation="DGLTYAAAVGRSFYEQILPAQSINLGWCSWAIQWLIRAPAPIPD
                     QVQVAYSADAATRAVYARQAAEDWQCFLRHRGAELASGGRLIVVTMALTEDGDFGYRP
                     VLDALYAALRDRVGEGFMSEAELHGMAIPTVGRSLPDIEAPFAAGSFAGLTIEDARLF
                     LAPDPIWEDYARNGDARAYGARWAAFSRSSVLPTLALALSDHGPERTLTFLGRVEAGM
                     ADRLAAAPRKFPIPLVIVDLRKA"
     gene            836..1699
                     /locus_tag="AC16A0_07875"
     CDS             836..1699
                     /locus_tag="AC16A0_07875"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTD2 family dehydratase"
                     /protein_id="HIR3105291.1"
                     /translation="MTDATLQSWIGRTEVRKDDILGFPVSAFAATLDRSDEISSAPGA
                     ELPALWVWLYFLPLAPMSEIGPDGHPRRGGFLPPIALERRMWAGSRCVFHGALKIGGQ
                     AERTSTILRIAEKTGKAGPMVFVTVRHEVRSGTGLVFEEEQDIVYVAMPDRPATPEPV
                     PLPPCDWQETVTVDPVLLFRFSALTFNGHRIHYDRDYATAVEKYPGLVVHGPLQALML
                     FEAARRRMPERRPARFEFRGIRPLFDKDSLTLSGRSRSDGGMDLFTGTASGATGMQAA
                     LHWQPDGSSHG"
     gene            1692..2912
                     /locus_tag="AC16A0_07880"
     CDS             1692..2912
                     /locus_tag="AC16A0_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3105292.1"
                     /translation="MARILEGLRVIEASAFVAAPLAGMTLAQMGADVIRFDRIGGGLD
                     HGRWPVTAQGKSLFWAGMNKGKRSLAIDISKPEGKEIVTRLICAPGADRGIFLTNLRA
                     RGWLDYDELRKHREDLIMMSVTGDRHGSPQVDYTVNPSVGFPTATGAEGSTEPVGHVL
                     PAWDCIAGQQAALGILAAERHRQRTGKGQLVEQALKDVALAMLGHLGIIGEVAINNYD
                     RPKLGNSLYGAYAQDFLCADGRRVMVVALTLRQWDNLCAATGMKVDCDALGQRLGVDL
                     RLEGERFKARHAITALLQPWFAARRVADFAADFTRRGVTWSEYRTFRAAVEQDSDCSI
                     ENPLFAMTEQPGIGRYLAPGLPSTFSALDRETPARAPVLGEHTDEILSELGYSRRELD
                     QLHAGKIIAGADSA"
     gene            3038..>3439
                     /locus_tag="AC16A0_07885"
     CDS             3038..>3439
                     /locus_tag="AC16A0_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3105293.1"
                     /translation="MTRNRICVIGLGSMGMGMAQAMLRKGLDVSGFDLNPEAVKRFES
                     MGGEGAAPAARAARDATILVSVVVNAAQTEAVLFGEDGAAAAMARGGVIISSATMSPD
                     DARRLAGRAEALGLHYLDAPISGGAARAAAGE"
BASE COUNT          567 a         1106 c         1184 g          582 t
ORIGIN      
        1 ggatgggttg acctatgccg cggccgtggg ccggagcttc tacgagcaga tccttcctgc
       61 gcagagcatc aatctcggct ggtgttcctg ggccattcaa tggctgatcc gggcgcctgc
      121 acccatacca gaccaggttc aggtggccta tagcgccgac gccgcgaccc gcgccgtcta
      181 tgcacggcag gccgccgagg attggcagtg ctttctccgg catcgcggtg ccgaactggc
      241 cagcggcggg cggctgatcg tggtgacgat ggcgctgacc gaggatggcg actttggcta
      301 ccgtccggtg ctcgacgcgc tctatgcggc actccgcgat cgcgttggcg agggattcat
      361 gagcgaggcc gaactccatg gcatggcaat cccgacggtc gggcgcagcc ttccggacat
      421 cgaggcgcca ttcgccgcgg gctcctttgc gggtctgacg atcgaagatg cgcgtctttt
      481 cctggcaccg gacccgatct gggaggacta cgcgcgcaac ggtgatgccc gggcttacgg
      541 cgcgcggtgg gccgccttct cgcggtcatc ggtgttgccc acgctggcgc tcgcgctgtc
      601 cgaccacggc ccggagcgga cgctgacatt ccttggccgc gtcgaggctg gaatggcgga
      661 tcgccttgcc gcagcgcccc gcaagtttcc gatcccgctg gtcatcgtgg acctgcgcaa
      721 ggcctgacat gctccgcact tcaaccgcgt ttgcttcagc caccacaagt ttgactcgcg
      781 gtccgcagga gagtgagcaa tagttcatgc ttgcaacgcg gcgcaacgga acaggatgac
      841 agacgcgaca ttgcagagct ggatcggccg aaccgaggtg cggaaggacg acatcctagg
      901 ctttccggtc agcgcctttg cggcaaccct cgaccgcagc gacgagatct cgtcggcacc
      961 aggtgccgaa ctgcctgccc tgtgggtgtg gctctacttc ctgccgctcg cccccatgtc
     1021 ggagatcggc cctgacggac atccccggcg cggtggcttc ctgccgccca tcgcactcga
     1081 gcggcgcatg tgggcgggca gccgctgcgt gtttcatggc gcgctgaaga tcggcggtca
     1141 ggccgaaagg acatccacca ttctgaggat cgcggagaag acgggcaagg cgggtccgat
     1201 ggttttcgtg acggtgaggc acgaggtccg cagcggcacc gggttggtgt tcgaagaaga
     1261 gcaggacatc gtctacgtcg ccatgccgga ccggccggcc acgccagagc ccgttccctt
     1321 gccgccctgc gactggcagg agacggtgac ggtggatccc gtgctgctgt tccgcttctc
     1381 ggccctcaca ttcaatggcc accgcatcca ctatgaccgc gactatgcaa ctgcagtcga
     1441 gaagtatccg gggctggtcg tgcatgggcc gctgcaggcg ctgatgctgt tcgaggccgc
     1501 acggcggcgg atgccggagc ggcggccggc gcgtttcgag ttccgcggca ttcgcccgct
     1561 gttcgacaag gacagcctga ccctcagcgg gcgcagccgc agcgatgggg gaatggatct
     1621 tttcaccggc acggcgagtg gcgcgacggg catgcaggcc gcattgcact ggcagccgga
     1681 cgggagcagc catggctaga attctcgaag gactgcgggt gatcgaagcc tccgctttcg
     1741 tggcggcgcc cctggccggc atgacgctgg cgcagatggg cgccgatgtg atccgtttcg
     1801 accgcatcgg cggcggactc gaccatgggc gctggccggt gacggcgcaa ggcaagagcc
     1861 tgttctgggc cggcatgaac aagggcaagc gcagcctggc catcgacatc tccaaaccgg
     1921 aaggcaagga gatcgtcacc cggctgatct gcgcaccggg agccgaccgc ggcatcttcc
     1981 tcaccaatct ccgcgcgcgc ggatggctcg actacgacga gctccgcaag catcgcgaag
     2041 acctgatcat gatgtcggtc acgggcgacc ggcatggcag cccgcaggtg gactacaccg
     2101 tcaatccatc ggtgggattt ccgaccgcga ccggcgcgga gggctccacc gaaccggttg
     2161 ggcatgtgct gccggcctgg gattgcatcg ccggccagca ggccgcgctc ggcatcctgg
     2221 ctgccgagcg ccatcgccag cgcaccggca agggacagct cgtcgaacag gccctcaagg
     2281 atgtggctct tgccatgctg ggccatctcg gcatcatcgg cgaggtggcc atcaacaact
     2341 acgaccggcc caagctgggc aattcgctct acggcgccta tgcgcaggac ttcctctgcg
     2401 ctgacggccg gcgcgtgatg gtggtggcgc tgacactgcg ccaatgggac aatctctgcg
     2461 cggcaaccgg gatgaaggtt gactgcgacg ccctgggcca gaggctcggt gtcgacctgc
     2521 gtctcgaagg cgagcgcttc aaggcgcggc atgccatcac cgctcttctg caaccatggt
     2581 ttgccgcgcg gcgtgtggcg gattttgccg cggacttcac ccggcgcggc gtgacctgga
     2641 gcgaataccg gaccttccgc gcggcagtcg agcaggattc cgactgttcg atcgagaacc
     2701 cgctcttcgc catgacggaa cagccgggca tcggccgcta tctcgcgccg gggctgcctt
     2761 ccacattttc ggcactggac cgggagacgc cggcacgagc gccggtactg ggcgaacata
     2821 ccgacgagat cctcagcgag ctgggctata gccggcgcga gcttgaccag cttcacgccg
     2881 ggaagatcat tgccggcgcc gattccgctt gaggcatgcc cgtgaagctt gtgctgcctg
     2941 cagcacgatg gcttgttgag tcccgcatgc ctgcgtaagg aggcgtaaca cgccggtgcg
     3001 tctcgtgcgg gcacaaatca gggagcagcg gccggtcatg acaagaaaca ggatctgtgt
     3061 gatcgggctg ggttccatgg ggatgggcat ggcgcaggcg atgctgcgca aggggctcga
     3121 tgtctcggga ttcgatctca acccggaagc ggtaaagagg ttcgagtcca tgggcggcga
     3181 gggcgccgcg cccgcggcac gggccgcacg ggatgcgacg attctcgtga gcgtcgtggt
     3241 caatgccgcg caaaccgagg ccgtgctctt cggcgaggat ggcgcagccg ccgccatggc
     3301 aaggggcggc gtcatcatct cgtcggccac catgtcgccc gacgatgcgc ggcggctggc
     3361 ggggcgcgcc gaagcgctcg gccttcacta tctcgacgcg cccatcagcg gcggcgcggc
     3421 cagggctgcg gccggcgag
//
LOCUS       DBNGVR010000370         3197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1707861, whole genome shotgun sequence.
ACCESSION   DBNGVR010000370 DBNGVR010000000
VERSION     DBNGVR010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1707861"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2995)
                     /locus_tag="AC16A0_07890"
     CDS             complement(<1..2995)
                     /locus_tag="AC16A0_07890"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR3105294.1"
                     /translation="MSKSTHSLRDLYDLSITCLFLTGPQAILRLVLMQSERDRQTGLN
                     TAGYVTGYRGSPVGTVDNQFAAARSVLAPRNITFLPGLNEDLAATAVWGTQQAEMRGE
                     GRFDGVFAVWYGKGPGVDRSGDVFRHANMAGTSPHGGVLAIAGDDHSGESSTVVHASD
                     VALSDAMIPVLSPAGVQEIIDYGLIGFAMSRYAGVWVGLKCVHDTVESSAVIEAGPER
                     GATVPPKDFTLPPDGLSIRPNDDRVPQEERLHAHKIPAVKAFARANAIDRIVMQGGRA
                     PKLGIVAAGKAYLDVRQALDDLGIDEAQAAKLGIRLLKIGMVWPLEPETVKRFAKGLD
                     TVMVVEEKRSLIEAQLRDILYAEEKRPSVIGKADENGRLLFAPFGVLEPNQIARAVAD
                     RIRDRAVTACASALQSASSARNQADLVARVPYFCAGCPHNSSTVLPDGARGYAGIGCH
                     WLAQFVPGRKTEGATHMGGEGANWVGEAPFSTRGHVFQNMGDGTYNHSGLMAIRHAVG
                     SGVNITYKILFNDAVAMTGGQRNDGGLTVPQIAQQMRAIGVERIAVVSDEPDKYPANA
                     GFPPFVTFNHRSELQAVQTELMGVKGTSVLIYDQTCAAEKRRRRRKGEFPDPARRVVI
                     NELVCEGCGDCGVQSNCVAIQPVETEFGRKRRIDQSSCNKDLSCLKGFCPSFVTVEGG
                     ELVKGSGPAVPDPESLPFPVLPAPELPRLDRPWSMLITGIGGTGVVTIGHLLGMAAHM
                     EGKGAALIDMVGISQKNGAVVTHLKIGAQPEDIAAVRVARGQADLILGCDLVTSASER
                     ILSAASRSRSHAVVNSHEVMPAHFTHDADFDVKGASLTLRVSAAVKPGGMHGIDATGI
                     ATKLLGDSIAANLFTLGFAWQKGLVPVSEQAIAEAVRLNGVGVKMNLAAFLWGRRAAV
                     DEAAVRAVIGGRPQGKSEDLAEIVARRAAFLTDYQNDAYADSYRHFVEQVRQTSEPLA
                     RAVAVNLFKLMAYKDEYEVARLHSDG"
     gene            3018..>3197
                     /locus_tag="AC16A0_07895"
     CDS             3018..>3197
                     /locus_tag="AC16A0_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR3105295.1"
                     /translation="MELPNASPARPTFRDAVGVWARIGCLSFGGPAGQIALMHREIVE
                     RRGWLSEKEYLSALNF"
BASE COUNT          525 a         1045 c         1093 g          534 t
ORIGIN      
        1 ggccatcgct gtgcagccgc gcaacttcat actcgtcctt gtaggccatc agcttgaaca
       61 ggttgacggc gacggccctg gccagcggct ccgacgtctg ccgcacctgc tccacgaaat
      121 gccggtagga gtccgcatag gcgtcattct ggtagtcggt caggaaagcg gcgcgacgcg
      181 ccacgatctc ggcaaggtcc tcggattttc cttgcgggcg cccgccgatc accgctctga
      241 ccgcggcttc gtccaccgcc gcgcggcggc cccacaggaa ggcagcgaga ttcatcttca
      301 cgcccacgcc gttcagcctg acggcttcgg caatggcctg ctcggatacc ggcacgaggc
      361 ccttctgcca ggcgaagccc agcgtgaaga gattggcagc gatggagtcg cccagaagtt
      421 tcgtggcgat cccggtggcg tcgatcccgt gcatgccgcc gggcttcacc gcggcggaga
      481 cccgcagcgt gagggacgcg cccttcacgt cgaagtcggc atcatgggtg aaatgcgccg
      541 gcatgacctc gtgactgttg accacggcat ggctgcggct gcgcgaggcg gcggagagaa
      601 tgcgctcgga ggcgctggtg acgagatcgc aaccgaggat gagatcggcc tggccgcggg
      661 ccacgcgcac ggcggcaatg tcttcgggct gcgcgccgat cttgagatgc gtgaccaccg
      721 ctccgttctt ctgcgagatg cccaccatgt cgatgagtgc tgcccccttg ccctccatat
      781 gcgcggccat gcccagcaga tgtccgatgg tgaccacgcc ggtgccgccg atgccggtga
      841 tcagcatgct ccagggccgg tcgagacggg gcagttcagg cgcgggcagc accgggaagg
      901 gcagcgactc gggatccggc acggcagggc cgctgccctt gacgagctcg ccgccttcca
      961 ccgtgacgaa gctggggcag aaacccttga ggcaggacag gtccttgttg cagctcgact
     1021 ggtcgatgcg gcgcttgcgg ccgaactcgg tctcgaccgg ctggatcgcc acgcagttgg
     1081 actgcacgcc gcaatcgccg cagccttcgc agaccagctc gttgatgacg acgcggcgcg
     1141 ccggatcggg gaattcgccc ttgcgccggc ggcggcgctt ctcggccgcg caggtctggt
     1201 cgtagatcag gacgctggtg cccttcaccc ccatgagttc cgtctgcacg gcctggagtt
     1261 cgctgcgatg gttgaaggtg acaaagggcg gaaagccggc attggcggga tacttgtccg
     1321 gctcgtcgga aaccaccgcg atccgctcga ccccgatggc ccgcatctgc tgcgcgatct
     1381 gcggcacggt gaggccgccg tcattgcgct ggccgccggt catggccacc gcgtcgttga
     1441 acaggatctt gtaggtgatg ttgacgccgg aaccgaccgc gtggcggatc gccatcagcc
     1501 cggagtgatt gtaggtgccg tcgcccatgt tctggaagac atggccgcgc gtcgagaagg
     1561 gcgcctcgcc cacccagttg gcgccttcgc cgcccatgtg ggtggcgcct tccgtcttgc
     1621 ggccgggcac gaactgcgcc agccagtggc agccgatgcc ggcatagccg cgcgcgccgt
     1681 cgggcagcac ggtggaggaa ttgtgcgggc agccggcgca gaaataggga acgcgggcca
     1741 cgagatcggc ctggttgcgg gcggacgagg cggattgcag ggccgaggcg caggcggtca
     1801 ccgcccggtc gcggatacgg tcggcaacgg cgcgggcgat ctggttcggc tccagcacgc
     1861 cgaagggcgc aaacagaaga cggccgttct cgtcggcctt gccgatgacg gacgggcgct
     1921 tttcctccgc atacagaata tcgcggagct gggcctcgat cagcgaccgc ttttcctcga
     1981 ccaccatcac cgtgtcgaga ccctttgcaa agcgcttgac ggtctcaggc tcgagcggcc
     2041 agaccatgcc gatcttgagc aggcggatgc cgagcttcgc tgcctgcgct tcgtcgatgc
     2101 cgagatcgtc caaggcctga cgcacgtcga gataggcctt gccggcggcc acgatgccga
     2161 gcttcggcgc cctgcccccc tgcatcacga tgcggtcgat ggcattggcg cgggcaaagg
     2221 ccttcacggc ggggatcttg tgggcgtgca ggcgctcttc ctgcggcacg cgatcatcgt
     2281 tcgggcggat ggacaggcca tcgggcggca gcgtgaagtc cttcggcgga acggtggcgc
     2341 cgcgttccgg accagcttcg atcacggcgg acgattccac cgtatcgtgc acgcatttga
     2401 gcccgaccca gacgccggca tagcgcgaca tggcgaagcc gatcaggccg tagtcgatga
     2461 tctcctgcac gcctgcgggg ctcagcacag ggatcatggc gtcgctgagg gccacgtcgc
     2521 tggcatggac gacagtggag gattcgcccg aatgatcgtc gccggcgatg gccagcacgc
     2581 cgccatgggg cgaggtgccc gccatgttgg catgccggaa gacatcgccc gaccggtcga
     2641 cgcccggccc cttgccatac cagacggcaa agacgccatc gaacctgccc tcgccccgca
     2701 tctcggcctg ctgcgtgccc cagacggcag tcgccgcaag atcctcgttg aggcccggca
     2761 ggaaggtgat gttgcgcggc gcgaggacgg atcttgccgc agcgaactgg ttgtcgacgg
     2821 tgcccaccgg cgagccgcgg taacccgtca catagccggc cgtgttcagc ccggtctggc
     2881 ggtcacgctc ggactgcatg agcaccaggc gcaggatcgc ctgcggcccg gtcaggaaca
     2941 ggcacgtgat cgaaaggtca taaagatccc tgaggctatg ggtcgatttg gacatgggct
     3001 ggactatact cactggcatg gaattgccaa acgcttcgcc tgcacggccg acattccggg
     3061 atgcagttgg cgtctgggcc cgaatcggct gcctgtcttt cggcggcccg gccggccaga
     3121 tcgcgctcat gcatcgcgag atcgtggaac gccgcggctg gctgagcgag aaggaatatc
     3181 tgtccgcgct caacttc
//
LOCUS       DBNGVR010000371         5802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2493983, whole genome shotgun sequence.
ACCESSION   DBNGVR010000371 DBNGVR010000000
VERSION     DBNGVR010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2493983"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..965)
                     /gene="pssA"
                     /locus_tag="AC16A0_07900"
     CDS             complement(150..965)
                     /gene="pssA"
                     /locus_tag="AC16A0_07900"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR3105296.1"
                     /translation="MSEAPSEPAAARPGRQGRFVPVRYLLPNLITLLALCSGITAIRL
                     AVEGRYELAVAAVIFAVVLDALDGRLARFLKGTSRFGAELDSLADFVNFGVAPALLIY
                     MWSLNALRPAGWVIALILAICCALRLARFNVALDDPDKPAWSAGFFTGAPAPAGAGLA
                     LLPMYLGFLGFVGDGHDYAVLIAPYIVLVAILMVSRIPTFSGKTMGKSIRREMVLPIL
                     GLAALGIIMLVTYPWHLLTVFAFAYLAMIPVGIRSYRRHKAQDRLQVEKSLKP"
     gene            complement(962..1666)
                     /locus_tag="AC16A0_07905"
     CDS             complement(962..1666)
                     /locus_tag="AC16A0_07905"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR3105297.1"
                     /translation="MSIVNSITAALVPIHREGYRFIAIFAAVTLVLFWLHLPTLGWVG
                     VLLTLWCAYFFRDPERVTPMRQGLVISPADGRISAIESVVPPPELDLPREPVTRISVF
                     MNVFDVHVNRSPVDAAITRITYVPGAFLNAELDKASADNERQALTLDIGEGRHIGVVQ
                     IAGLIARRIVKFVNEGDRLAAGQRFGLIRFGSRVDVYLPKGAVALACVGQRAIAGETV
                     LADLASSEGERSGRLA"
     gene            complement(1965..3788)
                     /locus_tag="AC16A0_07910"
     CDS             complement(1965..3788)
                     /locus_tag="AC16A0_07910"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABCB family ABC transporter ATP-binding
                     protein/permease"
                     /protein_id="HIR3105298.1"
                     /translation="MMPYLWPAGHSGLKARVIFAMVALVLSKVVTVATPYAFKYATDA
                     LSPAAGAAATVVLFFVLAYGFGRIMMVALAQLRDAVFAKVSQRAVRVLAVQTFRHLHA
                     LSLKFHLERRTGGLSRIVSRGTMGIDTVLRFSLFNTIPTILEIVLVAGLLVWSFGWSY
                     AAVIVITVILYVWYTYAATEWRMNIRRTMNDADTDANTKAVDSLLNFETVKYFGNEEH
                     EAERFGRSMETYEKAAIKTWVSLAVLNAGQALIFSIGLTIVMVMSALAVRNGQATVGD
                     FVLVNALMIQLYMPLNFIGSSYREIKQGLIDVEQMFQLLKVNAEIKDKPGATPLKVAE
                     AEVSFDTVSFAYDQERPILRDLSFTIPAGKTVAIVGPSGAGKSTVSRLLYRFYDVTGG
                     RILIDGQDIDGVTQKSLRAAIGMVPQDTVLFNDTIRYNIRYGRPDATDAEVEAAAKMA
                     QIHDFVMSLPKGYDSLVGEHGLKLSGGEKQRVSIARTILKGPAILILDEATSALDSFT
                     EHQIQEALKKVSENRTTLVIAHRLSTVVDADEIIVLDKGMVAERGTHEKLLRKQGIYH
                     AMWNRQREADQAREILKQTEEVPLEGSFRAKKPVTRKRVTT"
     gene            complement(3889..5328)
                     /locus_tag="AC16A0_07915"
     CDS             complement(3889..5328)
                     /locus_tag="AC16A0_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3105299.1"
                     /translation="MTEQSPTKKNPVLILTGLAIAGVAAVMGVTKDRWMPSEAPPAAT
                     EQAAQPAAQPETAPAASTTTEAQQPAAEQPAVEATAPAVTEQPAAETAAPAAPAQPAA
                     ETAGEVGEPASASGETPEQPAQVAAAPSAETSAPADAASPTVPTFDTVRVEKTGEAVI
                     AGRAKPGSEVTIMLDGQPIGTAVASADGSFVVVPEKPLPPGSGGISIVSKATGETTVT
                     KSDQTVAVIIPEAEKKQDALVAVVSPDQPTKVLQSPGMPEEQPQAAAAPAAPEEASAP
                     AAAGKARQIVLDAVDYDPSGNIVFSGQGEPGNNARIYVDNAPVGDAPIAADGRWSFSG
                     TARVTPGVHSLRIDSLDAGGSVTSRIEVPFFREDTRNVASAEPATEEGDTQKTDAQVP
                     AATAEPASAVPDEGRVVIQPGNNLWRISKVIYGDATKYTLLYQANQDQIRDPDLIYPG
                     QVFRTPDVVPPETIDPEARDPLTAEPAAQ"
     gene            5482..>5802
                     /locus_tag="AC16A0_07920"
     CDS             5482..>5802
                     /locus_tag="AC16A0_07920"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3105300.1"
                     /translation="MVPPRKLCVYCGSGNGRNPAYMAAARTLGKALADNNIGLVYGGG
                     SLGLMGETARSVLEAGGHVTGIIPEFLANRERMLTDGSELIVTADMHERKMTMFARAT
                     GFVAL"
BASE COUNT         1050 a         1818 c         1895 g         1039 t
ORIGIN      
        1 gccgaagccg acacgctaag cgatcccctt gactgccccg gtttcgagtc tagcccaagc
       61 agagatcatt tgctcgaagg aacttcacaa tactgtcgtt tcagcattct cgccagctca
      121 gcgcgcaatg ccggcaacca agagttgctt cacggtttga ggcttttttc gacctgaagc
      181 cggtcctgcg ccttgtgcct gcggtaggac cggatgccaa ccgggatcat cgcaaggtag
      241 gcaaacgcaa acacggtcag caggtgccac ggataggtca ccagcatgat gatgcccagc
      301 gccgcaaggc cgaggatcgg caggaccatt tcgcgccgga tcgacttgcc catggtcttg
      361 ccggaaaagg tcgggatgcg gctgaccatc aggatggcga caaggacgat atatggcgca
      421 ataagaaccg catagtcatg gccatcgccg acgaacccga ggaagcccag atacatcggc
      481 agcagcgcga gcccagcccc ggcgggcgcg ggcgcgcccg tgaagaagcc ggccgaccag
      541 gccggcttgt ccggatcgtc gagcgccacg ttgaagcggg caaggcgcag ggcgcagcag
      601 atggccagta tcagcgcaat cacccatccc gccgggcgca acgcgttgag cgaccacatg
      661 tagatcagca aggcgggcgc cacgccaaag ttcacgaaat cggcaaggct gtccagctcg
      721 gccccgaagc gcgacgtccc cttcagaaag cgtgcgaggc ggccatccag cgcatccagc
      781 acgacggcaa aaatgacggc tgcgaccgcc agctcgtacc ggccttccac cgcaagacgg
      841 atggccgtga tgccgctgca cagggccaga agcgtgatca gattggggag cagatagcga
      901 acagggacga agcggccttg acggcccggc cgcgcagcag ccggctcaga cggagcctcg
      961 ctcatgccag gcggccgctg cgctcgcctt cggacgaggc caggtcagca aggacggttt
     1021 cgccggcgat ggcacgttgg ccgacacagg ccagggcaac agctcccttg ggcaggtaga
     1081 cgtcgacccg gctgccgaaa cggatgagac cgaaccgctg ccctgcagcc agccggtcgc
     1141 cctcattgac gaatttcacg atgcggcgcg cgatgagccc ggcaatctgc accacgccaa
     1201 tgtgcctccc ctcgccgata tcgagggtca gggcctgccg ctcgttgtcg gcgctggcct
     1261 tgtcgagttc ggcattgagg aaggcaccgg gcacgtaggt gattcgggtg atcgcagcat
     1321 cgaccggcga gcggttcacg tgaacgtcaa acacgttcat gaagaccgag atgcgggtca
     1381 cgggttcgcg cggcaggtca agctcgggtg ggggaacgac ggactcgatg gcgctgatgc
     1441 ggccgtccgc gggcgagatc accagcccct gacgcatggg cgtcacccgc tcgggatcgc
     1501 ggaagaaata ggcgcaccac agcgtgagta ggacgccaac ccagcccagt gtcggtaggt
     1561 gaagccagaa cagcaccagg gtcaccgccg caaagatggc gatgaagcgg tagccttcgc
     1621 gatggattgg caccagggcc gcggtaatgg aattgacgat ggacatgtgg ctcatctagc
     1681 gcggtcgcgg cggtttgcat agcgtctgta cggaagggcg tccggccgga gggatctctc
     1741 gtggcgtgcg gcccggcgtt ccaccaccgg gccgtcacca tccccgttcc ggccagatcc
     1801 tcttgcggga cttcgccggc tgcatgacta cccccgagtc atgaggtcac catgatggaa
     1861 aaatgtaaac gaaaggaaat caccgtccag tttgtctcga attttcgagg accaagacgg
     1921 aaaacccagc ggcaggatta atgcccggtc gccaatcacg gtattcaagt ggttacccgc
     1981 ttgcgcgtga ccggtttctt cgcgcggaag ctgccttcaa gcggcacttc ctcggtttgc
     2041 ttgagtatct cgcgcgcttg gtcagcctcg cgctggcggt tccacatcgc gtgatagatg
     2101 ccctgtttgc gcaagagctt ctcgtgcgtt ccacgctcgg cgaccattcc cttgtcgagc
     2161 acgatgattt catctgcatc caccacggtg gacaggcgat gggcaatcac gagcgtggtg
     2221 cggttctcgg aaaccttctt cagagcctcc tgaatctggt gctcggtgaa gctgtcgagg
     2281 gctgacgttg cttcgtcgag aatcaggatg gccggtcctt tgagaatggt ccgcgcgatg
     2341 gagacgcgct gcttctcgcc gcccgagagc ttcagtccgt gctcgccgac cagcgagtca
     2401 tagcccttgg gcaggctcat cacgaagtca tggatctggg ccatcttcgc cgccgcctcg
     2461 acttcggcat ccgtcgcatc ggggcggccg tagcggatgt tgtagcggat cgtgtcgttg
     2521 aacagcaccg tgtcctgcgg caccattccg attgccgcac gcagagactt ctgcgtcacg
     2581 ccgtcgatgt cctggccatc gatgaggatg cggcccccgg tcacgtcata gaagcggtag
     2641 agcagccgcg acaccgtgga cttgccggcc cccgacggtc cgacgatcgc caccgtcttg
     2701 ccggccggaa tggtgaagga caggtcgcgc aggatcggcc tttcctggtc gtaggcgaaa
     2761 ctcacagtgt cgaaacttac ttcggcttcc gcaaccttca gcggcgtggc accgggcttg
     2821 tccttgatct cggcattgac cttgagcagc tggaacatct gctccacgtc gatcaggccc
     2881 tgcttgatct cgcgatagga cgatccgatg aagttcagcg gcatgtagag ctggatcatc
     2941 aatgcattga ccagcacgaa gtccccgacc gtggcctgac cgttgcgaac tgcaagggcc
     3001 gacatcacca tgacaatggt gaggccgata gagaagatca gcgcctgtcc ggcgttcagc
     3061 acggccagcg acacccaggt cttgatcgcc gccttttcgt aggtttccat cgagcggccg
     3121 aagcgttcgg cctcatgttc ctcgttgccg aaatacttca ccgtctcgaa gttgagaaga
     3181 ctgtccaccg ccttggtgtt cgcgtcggtg tcggcatcgt tcatggtgcg ccggatgttc
     3241 atccgccact cggtggcggc ataggtgtac cagacgtaga ggatcaccgt gatgacgatg
     3301 acggcggcat agctccatcc gaaagaccag accagcaggc ccgccaccag cacgatctcg
     3361 aggatggtcg ggatggtatt gaacagggag aagcgcagca ccgtgtcgat gcccatggtg
     3421 ccgcgggaca cgatgcgcga caggccgccc gtgcggcgct cgagatggaa cttcagcgac
     3481 aaggcatgga gatgccggaa cgtctggacc gccagcaccc gaaccgcgcg ctggctcacc
     3541 ttggcaaaga cggcatcgcg cagctgcgcg agcgcgacca tcatgatgcg gccgaagccg
     3601 taggccagca cgaagaacag caccacggtg gcggccgcac cggctgccgg gctcagcgca
     3661 tcggtggcat atttgaaggc atagggcgtc gccaccgtca cgaccttcga cagcaccagc
     3721 gcaaccatgg cgaagatcac ccgggccttc aggcccgaat ggccggccgg ccacagatag
     3781 ggcatcatgt cccgcaaaat ggcccattgg tcgccatccg accgtgatgg cgtgctcgta
     3841 tctgcgcgtg tcttgcggct cacgctttca cccgctctgg cgatccggtc actgcgccgc
     3901 gggttcggcg gtcagagggt cgcgggcttc gggatcgatg gtttccggcg gcaccacatc
     3961 gggcgtccgg aagacctggc cggggtagat caggtcggga tctcggatct ggtcctggtt
     4021 ggcctgataa agcagcgtgt acttcgtggc gtcgccgtag atgaccttgg agatgcgcca
     4081 cagattgttg ccgggctgga tcaccacacg gccttcgtcg ggcacggcgg aagcgggttc
     4141 cgcagtcgcc gccggtacct gggcatcggt cttctgggtg tcaccttcct ccgtagccgg
     4201 ctcggcgctg gccacgttcc tggtgtcctc gcggaagaag ggaacctcga tgcggctggt
     4261 cacgctgccg ccggcatcaa gactgtcgat gcgcagggaa tggacgccgg gcgtcacgcg
     4321 ggccgtgccc gagaagctcc accggccatc ggctgcgatc ggggcatcgc ccaccggggc
     4381 attgtccacg tagatgcggg cattgttgcc gggctcgccc tggcccgaaa agacgatgtt
     4441 gcccgaagga tcgtagtcca cggcatcgag cacgatctgc cgggccttgc cggccgccgc
     4501 aggtgccgag gcctcttccg gtgccgccgg tgccgctgcc gcctggggct gttcctccgg
     4561 catgccgggc gattgcagaa ccttggtcgg ctgatcgggg ctcaccacgg ccaccagcgc
     4621 gtcctgcttc ttctcggctt ctggaatgat gacggcgacc gtctggtcgg acttggtgac
     4681 agtcgtttcc cccgtcgcct tggacacgat gctgataccg ccgctgccgg gcggcagcgg
     4741 cttttcggga acgacgacga agctgccatc cgcacttgcg acggccgttc cgatcggttg
     4801 cccatccagc atgatggtca cttccgaacc gggcttggcg cggccggcga tgacggcctc
     4861 gccggtcttt tccacgcgaa ccgtgtcgaa ggtgggaacg gtgggggatg cggcatcggc
     4921 cggagcggaa gtttccgccg aaggagccgc ggcgacctgc gccggctgct cgggcgtttc
     4981 gcccgaggca gaggcaggct cgccaacctc gcctgccgtt tccgctgctg gttgcgccgg
     5041 ggcggcaggc gcggcggtct cggcggcggg ctgctccgtc acggcgggtg cggtggcttc
     5101 cacggcaggc tgttcggcag ccggctgctg ggcctcggtc gtcgtcgacg ccgccggagc
     5161 agtttccggc tgagcggcgg gttgcgctgc ctgttcggtg gcggcaggcg gcgcttccga
     5221 gggcatccag cgatccttgg tcacgcccat gaccgccgcc acgcccgcaa tggcgaggcc
     5281 ggtgaggatg aggaccgggt tcttcttggt gggcgactgt tctgtcatgt tcctgtgcct
     5341 tgaggcaaaa cgcgattccg cgaagggttc tggaggcctt aataaggcta ccgccacgat
     5401 ttcgcaaaga ttccctgcgc ctttccgcag ccactggcat ggtctaacaa ggctcttcaa
     5461 caggattgga gttcagaatc gatggttccc ccaagaaagc tctgcgtcta ttgcggctcc
     5521 gggaatggcc ggaatccggc ctacatggca gccgcccgca ccttggggaa ggccctggcc
     5581 gacaacaaca tcggactggt ctatggtggc ggcagcctcg gcctgatggg cgaaaccgcc
     5641 cgctcggtgc ttgaggctgg cggtcatgtc acgggaatca ttccggaatt tctggccaat
     5701 cgggagcgca tgctgaccga cgggagcgag ctcatcgtca ccgccgacat gcacgagcgc
     5761 aagatgacca tgtttgccag ggccaccggt ttcgtggccc tg
//
LOCUS       DBNGVR010000372         3581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3476608, whole genome shotgun sequence.
ACCESSION   DBNGVR010000372 DBNGVR010000000
VERSION     DBNGVR010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3476608"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..554)
                     /locus_tag="AC16A0_07925"
     CDS             complement(117..554)
                     /locus_tag="AC16A0_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3105301.1"
                     /translation="MIDTRLAPYGAFVLRAALGVMFIAHAYLKLGVFTVAGFEGFLTS
                     IGLPAALAWPIILAELLGGAALLLGVYSRWVALATLPILLGALVIHAPNGWVFNAPNG
                     GWEYPAFLAAAAVAHVLIGEGAFALKRAGLPLVADSTASAVRA"
     gene            730..1671
                     /locus_tag="AC16A0_07930"
     CDS             730..1671
                     /locus_tag="AC16A0_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HIR3105302.1"
                     /translation="MGTTGIHHVTAIAGNARRNLDFYTRTLGLRLVKRTVNFDDPGTY
                     HFYFGDAGGSPGSIITFFPWDHVTSGRLGTGETQETALRVPEAALGFWLERFVAMGVS
                     HEPVATRFGARTLPFKDPDGMALALVAVPGAQSEPAWSDGSVPEEFAIRGIHGVTLLL
                     AEAGATAKILADVLGFAEERTEGAVTRLRSPASQIGGIVDLRAVGGFPRGRSGGGSVH
                     HIAFRAADDAAQAEMVRKLETAFGIRPTPQKDRTYFRSVYFREPGGVLFEIATDVPGF
                     AVDEPPAHLGEALKLPAFLEARRAEIAARLPDLGDDA"
     gene            1668..2609
                     /locus_tag="AC16A0_07935"
     CDS             1668..2609
                     /locus_tag="AC16A0_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3105303.1"
                     /translation="MSDDLRSISWDDIRLVKAVANARGLAGAARTMGLNHSTVFRRLA
                     QIEDRAGTALFVRHRTGYAATAAGEEMVALAERMEDDVASLSRKLAGGAVSPGGELRV
                     TTNDTLLVGLLTPIFAGFQRQCHDIRLDIVLGNQALNLSKRDADVAIRATDSPPDTLF
                     GRRVAHIAWALYGREQDFPEPVVQPIEPLLGHRWIALGDALGSFKAAKFVREHVAAER
                     IVYKLNTVLGLTEAVEQGIGIGHLPCFIADRRPGLRRLSPAVPDYGVDLWLLTHPDLK
                     NVPRVRIFMDFVAAEIARQRPLIEGERGHGAHMVSGD"
     gene            2667..>3581
                     /locus_tag="AC16A0_07940"
     CDS             2667..>3581
                     /locus_tag="AC16A0_07940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR3105304.1"
                     /translation="MTASAELEIFLVALPGLESVLCDEAREKGFTSSRIVPGGVAFRG
                     SWPDVWRANLEIRGASKVLVRIGAIRVLHLAQLDKRARRFPWAETLRRDVPLRVDVTC
                     RKSRIYHAGAAAQRIATAIREELGAPVTPDAELCIKARIEDDLCSFSIDSSGELLHKR
                     GHKQAVGKAPMRETMAALFLQQCGYAGHEPVLDPMCGSGTFVIEAAEIAAGLQPGRSR
                     RFAFESLAGFDAAHWSRLQSACAPKTPAVHFYGSDRDAGAIRMSRDNAARAGVSDYTT
                     FRKQSISELVRPDGPPGLVIVNPPYGDRI"
BASE COUNT          608 a         1182 c         1172 g          619 t
ORIGIN      
        1 cttgaccgga cggacggaca tggcggaact cctcgtgttg ctgtgtctct ctaaacgtca
       61 tccggcggca caccgcaagc gggcgccgcc ggttcaatgg cagatgcagt cagcgcttac
      121 gcacgcaccg cgctggcggt gctgtccgca accaggggaa gaccggcccg cttcagggcg
      181 aaggcgcctt cgccgatcag cacatgggcc actgcggcag ctgccaggaa agcgggatac
      241 tcccacccgc cgttcggggc attgaagacc cagccgttgg gggcatggat gaccagcgcg
      301 cccaggagga tcggcagtgt cgcaagcgcc acccagcgcg aatagacgcc gaggagcagg
      361 gctgcgcctc ccagaagttc ggccaggatg atgggccagg ccagggccgc gggcaggccg
      421 atgctggtga ggaagccttc gaagccggca acggtgaaga cgccaagctt cagataggca
      481 tgggcaatga acatgacgcc gagggctgca cgcaggacaa aggcgccata gggggcaagg
      541 cgggtgtcga tcattttcgg tctccgtgtt tgaatgatgt gccccgcata ttgctcttgc
      601 gtctacgcaa gtaaatcagc aataatgcaa tttaatcttt gcatgaatgc aaaggttgag
      661 tacttcgtgg gcaggcgacc ttgccctatg tgccgtccat cgctgaaccc tcgtcgacag
      721 gagacactca tgggcaccac gggcatccat cacgttacgg caatcgccgg caatgcgcgc
      781 cgcaatctcg atttctatac ccggacactc ggcctgcgcc tcgtcaagag gacggtgaac
      841 ttcgacgatc ccggcaccta tcacttctac ttcggcgatg cgggcggtag ccccggcagc
      901 atcatcacgt tcttcccctg ggaccatgtg acctcgggcc ggctcggcac gggcgagacc
      961 caggaaaccg cattgcgcgt tccggaagca gcactcggct tctggctgga gcgcttcgtg
     1021 gccatgggcg tgagccatga accggtcgca acacgcttcg gggcgcgaac cttgcccttc
     1081 aaggatccgg acggcatggc gctggcgctc gtggcggttc ccggcgcgca atccgagccg
     1141 gcctggagcg atggttccgt ccctgaggaa ttcgccatcc gcggcatcca cggcgtcacc
     1201 ctcctgctgg cggaagccgg ggcgacggcg aagatccttg ccgatgtgct tggctttgcg
     1261 gaagagcgca cggagggcgc cgtcacccgc ctgcggtcgc cggcctcgca gatcgggggc
     1321 atcgtcgacc tgcgcgccgt ggggggcttc ccgcgcggac gttccggcgg cggctcggtg
     1381 catcacatcg ccttccgcgc tgccgacgat gcggcgcagg ccgagatggt gcgcaagctc
     1441 gaaacagcct ttggcatccg gcccacgccg cagaaggacc gcacctactt ccgctcggtc
     1501 tatttccgcg agcccggtgg cgtgctcttc gagatcgcca ccgacgtgcc gggctttgca
     1561 gtggacgagc cgccggcgca tctcggcgaa gcgctcaagc tgccggcatt cctcgaggca
     1621 cggcgcgcgg agatcgcggc gcgcctgccc gatctcggag atgatgcatg agtgacgatc
     1681 tgagatccat atcctgggat gacatccgac tcgtcaaggc ggttgccaac gcgcgcggcc
     1741 ttgcgggtgc ggcccggacg atgggtctca atcattcgac cgtcttccgc cgcctcgcgc
     1801 agatcgagga ccgtgcaggc actgcgctct ttgtccgcca ccgcacgggc tatgccgcca
     1861 cggccgccgg cgaagagatg gtggcactgg ccgagcgcat ggaagacgat gtggcaagcc
     1921 tcagccgcaa gctcgcaggc ggcgcagtct cccccggcgg cgagttgcgg gtcaccacca
     1981 acgacacgct cctggtcggg ctgctgacgc cgattttcgc cggcttccag cggcagtgcc
     2041 acgacatccg gctcgacatc gttctgggca atcaggcgct caatctctcg aagcgggatg
     2101 ccgacgtggc gatccgcgcc accgattcac cgcccgacac gctgttcggg cgccgcgtgg
     2161 cgcatatcgc ctgggctctc tatggccgcg agcaggattt ccccgagccc gtcgtccaac
     2221 ccatcgagcc gcttcttggg catcgctgga tcgcgctggg cgatgcgctc ggcagcttca
     2281 aggcggcgaa gttcgtgcgc gaacacgttg ccgccgaacg catcgtctac aagctcaata
     2341 cagtgctggg gctgacggag gccgtcgaac agggcatcgg catcggtcat ctgccctgct
     2401 tcatcgccga ccgcaggccc gggctccgcc gcctctcgcc tgccgtgccg gactatggcg
     2461 ttgatctctg gttgctcacg caccctgacc tgaagaatgt gccgcgcgtg cgcatcttca
     2521 tggatttcgt cgcggcggaa atcgcgcggc agaggcccct gatcgaaggc gagcgtggcc
     2581 atggcgcgca catggtttcg ggtgactgac accgccgcca tcgaaacctg tcgaagcctg
     2641 ctcgaattcc ggatatggga cttgcaatga ccgcgtccgc cgagcttgag atcttcctcg
     2701 tggccctgcc tggcctcgag tccgtgctgt gcgacgaggc acgcgagaag ggatttacgt
     2761 cgtcgcgaat cgtgccggga ggcgtggcct tccggggcag ttggccagat gtctggcggg
     2821 ccaacctcga gattcgcggg gcaagcaagg tactggtccg catcggcgcc atccgtgtgc
     2881 ttcatctggc gcagctcgac aagcgggcgc gccgttttcc ctgggccgag acgctgcgcc
     2941 gcgacgttcc gctgcgcgtc gacgtcacct gccgcaagtc gcgcatctat catgccgggg
     3001 ctgccgcgca gcgcatcgcc acggcaatcc gcgaggaact tggcgcgccc gtcacgcccg
     3061 atgccgaact ctgcatcaag gcccgcatcg aggacgatct ctgcagcttc agcatcgata
     3121 gctcgggcga gctcctgcac aagcgcggcc acaagcaggc ggtcggcaag gcgccgatgc
     3181 gcgagaccat ggcggcgctg ttcctgcagc aatgcggata tgcgggccat gagccggtcc
     3241 tggatccgat gtgcggatcg ggtacattcg tcattgaggc cgcagagatt gcagcgggtc
     3301 tgcaacccgg ccggtcgcgg cgctttgcct tcgagagcct ggccggattc gatgccgcgc
     3361 attggtcgcg tctgcagtcg gcttgcgcgc cgaaaacacc ggccgtccac ttctacggca
     3421 gcgatcgcga tgccggtgcc atccgcatga gccgggacaa tgcagcgcgc gccggcgtct
     3481 cagactacac gacattccgc aagcaatcga tcagcgaact ggtgcggccc gatgggcccc
     3541 cgggcctggt catcgtcaat ccgccctatg gtgatcgcat c
//
LOCUS       DBNGVR010000373         3246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4066440, whole genome shotgun sequence.
ACCESSION   DBNGVR010000373 DBNGVR010000000
VERSION     DBNGVR010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4066440"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="AC16A0_07945"
     CDS             <1..597
                     /locus_tag="AC16A0_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017271579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HIR3105305.1"
                     /translation="ATDPDEMMRNHDVIVKHGGNCAVININSIGFGAFAFLRKRSGLV
                     LHAHRNGWDILTRHPGLGMDFRVWQQFWRLLGVDQFQINGIRVKYWEPDESFVRSFKA
                     ITTPLFTPADCPLPVAGSGQWGGQAPETYQRTGRTLDLLYLCGGGIVSHPGGAGAGVK
                     AVRQAWEAAVAGIPLEDYAKDHPELAQSIAAFAGGKGA"
     gene            594..1874
                     /locus_tag="AC16A0_07950"
     CDS             594..1874
                     /locus_tag="AC16A0_07950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HIR3105306.1"
                     /translation="MTRLLSYYGDDFTGSTDVMEALATHGIDTVLFTRLPGLAEFAPF
                     ASYPAIGLAGTSRSETPAWMSETLPPIFAWLKSLDARFCHYKVCSTFDSSPMIGSIGR
                     AAEIGAACFAQALIPLVVGAPQLKRYTFAAHLFAAYQGAIYRIDRHPVMSRHPVTPMD
                     ESDLRLHLARQTGLPVVLAGTDWPSRGIALLDVHDDATQLVAGCRLLALPSHAAPIVI
                     GSSGVEYALMRALADAGTISGRAAFAPLARVEQTMVVSGSVSPTTERQIRHALAQGFE
                     AVPISAVALACGEPQVMAEAVERSLGILQRGLSPLVHTALGAATDEGARIDASSDGRR
                     NIGEGLGRILGGVLARTGLSRVMIAGGDTSSHALGQLDIFALTTRHPLPATPGSPLCN
                     AASALPRLNGIEIALKGGQVGGDDYFVSLRDGRV"
     gene            1992..2168
                     /locus_tag="AC16A0_07955"
     CDS             1992..2168
                     /locus_tag="AC16A0_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105307.1"
                     /translation="MSKPFRTLATPLRCTFNAFSSYVERRRRVADLRNLDKTLLRDAG
                     LSQPEIDDLRRVFF"
     gene            complement(2232..3140)
                     /locus_tag="AC16A0_07960"
     CDS             complement(2232..3140)
                     /locus_tag="AC16A0_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3105308.1"
                     /translation="MSVRPVKSITTARPAIEGAGVKLHRVFGFGDTKPFDPFLMMDDF
                     RNDRPDDYLAGFPWHPHRGIETITYVLAGEVDHGDSLGNRGTLGAGDVQWMTAGRGIM
                     HQEMPRGDGLGRMHGFQLWANLPSSLKMTAPRYQDIKAAAIPDVVDDDGTRVKVICGD
                     FWGKTGPVDGIAAEPRYLDVSVPPGKRKTLKVEVERHAFAYVFAGSGDFRAASKPFGV
                     LTEKEVNGEEIHVRETTGNRSLVLFDSGDEITVQAGDEGVRFLLVSGKPIKEPVAWYG
                     PIVMNTQAELQQAVSELRNGTFIRSQ"
BASE COUNT          514 a         1117 c         1028 g          587 t
ORIGIN      
        1 gcgaccgatc cggacgagat gatgcgcaac catgacgtga tcgtgaagca cggcggcaac
       61 tgcgccgtca tcaacatcaa ttccatcggc ttcggggcct tcgccttcct gcgcaagcga
      121 tccggcctcg tgttgcatgc gcatcgcaac ggttgggaca tcctcacccg tcatcccggc
      181 ctcggcatgg atttccgggt gtggcagcag ttctggcggc tgctcggcgt cgatcagttc
      241 cagatcaacg gcatccgcgt gaagtactgg gaaccggacg aaagcttcgt gcgctccttc
      301 aaggcgatca ccacaccgct cttcacgccc gccgactgcc ccctgccggt ggcaggctcc
      361 ggccagtggg gcggccaggc gccagagacc taccagagaa ccggccggac actcgatctt
      421 ctctatctct gcggcggagg gatcgtctcg catcccggcg gtgcgggtgc gggcgtgaag
      481 gcggtgcgcc aggcctggga agcggcggtt gccggcatcc cgctggagga ttacgcgaag
      541 gaccatccgg aactggctca gtccatcgcc gccttcgccg gcgggaaggg cgcatgacgc
      601 ggcttctctc ctattatggc gacgacttca ccggctccac cgacgtgatg gaggcgctgg
      661 ccacccatgg catcgacacg gttctcttca cccgcctgcc agggcttgca gaattcgcgc
      721 ccttcgcgtc ctatcctgcc attggccttg ccggaaccag ccgcagcgag acgcccgcat
      781 ggatgagcga gacgcttccg cccatcttcg cctggctgaa gtcgctcgac gcccgctttt
      841 gccactacaa ggtctgctcg accttcgact ccagccctat gatcggcagc atcggccgcg
      901 cggccgagat cggcgcggcc tgttttgcgc aggcgctgat cccgctggtt gtcggcgcgc
      961 cgcagctgaa gcgctatacc ttcgccgccc atctcttcgc ggcctaccag ggcgcaatct
     1021 atcggatcga ccggcaccct gtgatgtcac gccatccggt cacgccgatg gacgagtccg
     1081 atctgcgcct ccacctggcg cggcagaccg gcctccccgt cgttctcgcc ggtaccgatt
     1141 ggccatcgcg cggcattgcg ctgctcgatg tccatgacga tgcgacccag ctggtggccg
     1201 gctgccgcct cctcgccttg ccgtcacatg ccgcgcccat tgtcatcggg tcctcgggcg
     1261 tcgaatatgc cttgatgcgg gcgcttgccg atgcgggcac catatcggga cgcgccgcat
     1321 tcgcgccgct cgcaagggtg gagcagacca tggtcgtctc cggcagcgtt tcgcccacca
     1381 ccgagcgtca gatccgccat gcccttgcgc agggtttcga ggccgttccg atttccgccg
     1441 tggcgctggc atgcggcgaa ccgcaggtga tggccgaagc ggttgaacgc tcgctcggga
     1501 ttctgcagcg cgggctgagc ccgctggttc acacggcgct tggcgcggcc accgacgagg
     1561 gcgcacgcat cgatgccagc agcgacggcc gccgcaacat cggcgagggt cttggtcgca
     1621 ttctcggagg cgttctcgca aggaccggcc tcagccgcgt catgatcgca ggcggcgaca
     1681 cgtcgagtca cgcgctgggc cagcttgaca tcttcgccct cacgacgcgg caccccttgc
     1741 ccgcgacgcc cggctcgccg ctgtgcaatg ccgcatcggc cttgccgcgg ttgaacggga
     1801 tcgagattgc cctcaagggc ggacaggtgg gtggcgacga ctactttgtc agcttgcgcg
     1861 acggcagggt ctgaccgcga ccgccccgac cgatcgcgca ggccggacct gagtgatgcg
     1921 ggtcacagac ggacggcccg gaatccctaa ggtcatgagc gtcgtctgtt gtcaccggtc
     1981 aggaggctgc catgtcgaag ccgtttcgaa ctctcgccac gccgctgcgc tgcaccttca
     2041 acgccttcag cagctacgtc gaacgccgcc gcagggtggc tgacctccgc aacctcgaca
     2101 agaccctttt gagagacgcg ggactctcgc agcccgaaat cgacgatctg cgccgcgtct
     2161 tcttctagcc gcccgccggc cgggtgccgc gtcgtcggcg cggcgttccg gccggccggg
     2221 tgcggctgtt ctcactgcga ccgaatgaag gttccgttcc gcaactcgct gaccgcctgc
     2281 tgcagctcgg cctgcgtgtt catcacgatg ggcccatacc aggccaccgg ctccttgatg
     2341 ggctttccgg acacgagaag gaaccggaca ccttcatcgc cggcctgaac cgtgatctcg
     2401 tcgccgctgt cgaacagcac aagcgagcgg ttgccggtgg tctcgcgcac gtggatctcc
     2461 tcgccgttca cctccttctc ggtaagcacg ccgaaaggct tcgatgccgc gcggaagtca
     2521 ccggagcctg cgaagacata agcgaatgca tgacgctcta cttccacttt cagtgtcttg
     2581 cgcttgcccg gtggcaccga gacatcgaga tagcgaggct cggcggcaat cccgtcgacc
     2641 ggcccggtct ttccccagaa gtcgccgcag atgaccttca cccgcgtgcc gtcgtcatcg
     2701 acgacatcgg gaatcgcggc cgccttgatg tcctggtagc gcggtgccgt catcttgagc
     2761 gacgacggca ggttggccca gagctggaag ccatgcatgc ggcccagccc gtcgccgcgc
     2821 ggcatttcct ggtgcatgat gccgcgccct gcggtcatcc actgcacatc acctgcgccg
     2881 agggtgccgc ggttgccgag gctgtcgccg tgatcgacct cgccggccag cacataggtg
     2941 atggtctcga tgccacgatg cgggtgccag gggaaaccgg ccaggtaatc gtcgggacgg
     3001 tcgttgcgga aatcatccat catcaggaag ggatcgaagg gcttggtgtc gccgaagccg
     3061 aagacgcgat gaagcttcac gccggcccct tcgatggcgg ggcgcgcggt ggtgatgctc
     3121 ttgaccggac ggacggacat ggcggaactc ctcgtgttgc tgtgtctctc taaacgtcat
     3181 ccggcggcac accgcaagcg ggcgccgccg gttcaatggc agatgcagtc agcgcttacg
     3241 cacgca
//
LOCUS       DBNGVR010000374         3865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4458731, whole genome shotgun sequence.
ACCESSION   DBNGVR010000374 DBNGVR010000000
VERSION     DBNGVR010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4458731"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1715
                     /locus_tag="AC16A0_07965"
     CDS             195..1715
                     /locus_tag="AC16A0_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105309.1"
                     /translation="MSFRDHDKKTFIADTARAYAERRISKRDFMKKMSLAGVGFSAFA
                     AGLLGNTRPYRGNLGLLGTEALAQDASVTQWLKDVGAPFKGTKIRYTSEATPPTVVLD
                     KLKNEFTEPTGIEVEIEIVPLEQVLAKATQDVQGQLGTYDLYYLDQSWVATFAQDTID
                     PVAYYKEKPDLAMPGFDFDDFSKPLVSGLAEYEGKWVGIPFDIPIFMLMYRKDILEKH
                     GIAVPTSPAEFTAAIQKITEAEKANGMFGTGLQAKSGHYSLNCDWTQMVWNEGGSIFG
                     ADKKFKGNDEAGVAGLKLYQEWIKNAPPESLNSTWDGQFQMMAAGQCALVQSWDEFFP
                     GLDADDSKVKGLWMPAKPLVGKPLRPLSEVGFGEIPNYAHQGGSILGLSKYSKNIDPA
                     WLFMQWACSKDIMTRCTLLGGFAPMRISSFRDPRVKEKAVVGPGTTRHLEMVEWIIDN
                     NMASEPDMPLWAGFSNNEIPVNLGKLLTGQDFNGDAKACMDTIAASIDAAVAESGL"
     gene            1773..3293
                     /locus_tag="AC16A0_07970"
     CDS             1773..3293
                     /locus_tag="AC16A0_07970"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3105310.1"
                     /translation="MRGDLVIGIDSSTTATKAIAWDRRGRAVAEGRAAIPLSNPRPGW
                     FEQKAEDWTKALTASLRQLARKIDMTRVAALAISNQRESFAQFDRNDRALRPGTLWLD
                     ERAPAEARELAAKIGAERIRAISGKPSDVTPCISRCAWFARHMPGLWKKTAMTAEVHG
                     VLTHFLTGHWHTSTASADPMGLIDMARYDWSDELISAVGLTRDQLPKLFRPGDAMGEV
                     TAAAAKATGLKPGTPVIAGGGDGQCAGTGTNIFVTGRAYVNIGTACVSGSAGSRYAHA
                     QAFRTLTTVAEDGYIYETCLRTGTFLINWTVETLFNAKGSPALFKTLEAEAAAAPVGA
                     NGIVVMPHWSGSMTPFWDSTTRGVIAGLSASHRRGDVYRAVLEGIALEQAMMTNLAAA
                     ASQPIDHYVAIGGGAKSDLLCQILADASGRAVKRLATVEASSLGAAVAAAKGAGWFKS
                     IPQAAAAMAGKPVRTFRPSAKSQRRYQELLAIYTDLYPAIRDWNARLDAFAAKDRA"
     gene            3290..>3865
                     /locus_tag="AC16A0_07975"
     CDS             3290..>3865
                     /locus_tag="AC16A0_07975"
                     /inference="COORDINATES: protein motif:HMM:NF025065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3105311.1"
                     /translation="MTDWNALISDVVAGNWKDPSTGKAAIVPFETIRIEETLDGGEAD
                     VLAPLKLGKRLAVVCDTNTVEAMGRRVAKHLKGLGTVDEIVLPSTLECDEPTIALVQE
                     KTRHADAIVAVGSGALSDTCKYATFKDGRKYATFGTAASMNGYAASTASVTLNNGYKT
                     SLPAHAPRGIFLDLQVSAAAPHWLSAAGLGDS"
BASE COUNT          708 a         1343 c         1183 g          631 t
ORIGIN      
        1 agatccagac cttcgtgact tcgacgggca atgaatgggg cttcatcgcg gcgctgggaa
       61 cggccgccgt gatcccgagc ttcatcttca tcctgcttgt ccaacgccat ctggtgcgcg
      121 gcctcactct tggctccctg aaggagtgag gccgttgcgc gagaacctaa gctcgaaccc
      181 atgggaggat tacaatgagc tttagagacc acgacaagaa gacctttatt gccgatacgg
      241 cgcgcgccta tgccgaacgc cgcatctcaa agcgcgactt catgaagaag atgagtctcg
      301 ccggcgtcgg cttctcggcc ttcgctgccg gactcctcgg caacacccga ccctatcgcg
      361 gcaacctggg cctgctcggc accgaagcgc tggcgcagga cgcctccgtc acgcaatggc
      421 tgaaggatgt gggcgccccc ttcaagggca ccaagatccg ctacacctcg gaagcaaccc
      481 cgccgacggt cgttctcgac aagctgaaga acgagttcac cgagccgacc ggcattgaag
      541 tcgaaatcga gatcgtgccg ctcgagcagg ttctcgccaa ggcgacgcag gacgtgcagg
      601 gccagctcgg cacctacgat ctctactacc tcgaccagtc ctgggttgcg accttcgctc
      661 aggacaccat cgatccggtg gcctactaca aggaaaagcc ggacctggcc atgccgggct
      721 tcgacttcga cgacttctcc aagccgctgg tctcgggtct tgccgagtac gagggcaagt
      781 gggtcggcat tcccttcgac atcccgatct tcatgctgat gtaccgcaag gacatcctcg
      841 agaagcacgg catcgcggtc ccgacctctc cggccgaatt caccgctgcg atccagaaga
      901 tcaccgaggc cgagaaggcc aatggcatgt tcggcaccgg cctccaggcc aagtcgggcc
      961 actactcgct gaactgcgac tggacccaga tggtgtggaa cgaaggcggc tcgatcttcg
     1021 gcgccgacaa gaagttcaag ggtaacgacg aagccggcgt tgccggcctg aagctctacc
     1081 aggagtggat caagaacgcg ccgcccgagt cgctcaactc gacgtgggac ggccagttcc
     1141 agatgatggc cgcgggccag tgcgccctcg tccagtcctg ggacgagttc ttcccgggcc
     1201 tcgacgccga cgactccaag gtcaagggtc tgtggatgcc ggccaagccg ctggtcggca
     1261 agccgctgcg tccgctttcc gaagtgggct tcggcgaaat cccgaactat gcccaccagg
     1321 gcggctcgat cctcggcctg tcgaagtact cgaagaacat cgacccggcc tggctcttca
     1381 tgcagtgggc ctgctcgaag gacatcatga cgcgctgcac gctgctcggc ggcttcgcgc
     1441 ccatgcgcat ctcgtccttc cgcgacccgc gggtgaagga aaaggccgtc gtcggtccgg
     1501 gcaccacccg ccacctcgaa atggtcgagt ggatcatcga caacaacatg gcttctgagc
     1561 ccgacatgcc gctctgggcc ggcttctcca acaatgaaat tccggtcaac ctgggcaagc
     1621 tgctcacggg ccaggatttc aacggagacg ccaaggcctg catggacacg attgccgctt
     1681 cgatcgacgc ggcagttgcc gagtccggcc tctaagacac gccacggcgg aggcggcatc
     1741 atgccgcctc cgcctctctc atgcgggcac atatgcgcgg cgaccttgtt atcggtatcg
     1801 actcctcgac aacggcaacc aaggccatcg cctgggaccg ccgtggccgt gccgtggccg
     1861 aaggtcgggc tgccattccg ctttccaatc cgcgccccgg ctggttcgag cagaaggccg
     1921 aggactggac caaggcgctg accgcgtcgc tgcgccagct cgcgcgcaag atcgacatga
     1981 cgcgcgtggc cgcgctggcc atttccaacc agcgcgaaag ctttgcgcag ttcgaccgga
     2041 acgaccgggc gctgcgtccg ggaaccctct ggctcgacga gcgcgcgccc gccgaggcgc
     2101 gcgagcttgc cgccaagatc ggcgccgaac gcatccgcgc gatctccggc aagcccagcg
     2161 atgtcacgcc ctgcatctcg cgctgtgcct ggttcgcccg gcacatgccc ggcctatgga
     2221 agaaaaccgc catgaccgcc gaggtgcatg gcgtgctcac ccatttcctg accggccact
     2281 ggcacacctc gaccgcctcg gccgatccca tgggtctcat cgacatggcc cgctacgact
     2341 ggtcggacga acttatctcc gccgtgggac tgacgcgcga tcaactgccg aagctctttc
     2401 gtcccggcga cgccatgggc gaggtgacgg ctgcggcggc caaggccacg ggcctgaagc
     2461 ccggcacacc agtcattgcc ggcggcggcg acgggcaatg cgcgggcacc ggcaccaaca
     2521 ttttcgtcac gggccgcgcc tatgtgaaca tcggcacggc atgcgtttcg ggaagcgccg
     2581 gcagccgcta tgcacatgcc caggcgttcc gcaccctgac gaccgtcgcg gaggacggct
     2641 atatctacga aacctgcctc cgcaccggca ccttcctgat caactggaca gtggagacgc
     2701 tcttcaacgc caagggatcg cccgcgctgt tcaagacgct cgaggccgaa gcggcggccg
     2761 cccctgtcgg cgccaacggc atcgtggtca tgccgcactg gtcggggtcg atgacgccct
     2821 tctgggactc gacgacgcgc ggcgtcatcg ccggactctc cgcctcgcac cgccgcggcg
     2881 atgtctatcg cgccgtactg gagggcatcg cgctggaaca ggcgatgatg accaacctcg
     2941 ccgcggcggc gagccagccc atcgatcact atgtggcgat cggcggcggc gccaagtcgg
     3001 acctgctgtg ccagatcctc gccgatgcct cgggccgcgc cgtgaaacgc ctcgccacag
     3061 tggaggcttc gtcgctaggc gctgccgtcg cggccgccaa gggcgctggc tggttcaaat
     3121 ccattccgca ggcagccgcc gccatggcgg gcaagcccgt caggacattc cggccctccg
     3181 cgaagtcgca gcgccgctac caggagcttc tcgccattta caccgatctt tatcccgcca
     3241 tccgcgactg gaacgccagg ctcgatgcct ttgccgcaaa ggaccgcgca tgaccgactg
     3301 gaacgccctc atcagcgatg tcgtcgccgg aaactggaag gatccgtcca ccggcaaggc
     3361 cgccatcgtc cccttcgaga ccatccgcat cgaggagaca ctcgacggcg gcgaggccga
     3421 cgtgctggcg ccgctgaagc tcggcaagcg gcttgccgtt gtctgcgaca ccaataccgt
     3481 cgaagccatg ggccgccgcg tggccaagca cctcaagggc ctcggcactg ttgatgagat
     3541 cgtgctgccg tccacgctcg aatgcgacga gccgacaatc gcgctggtgc aggagaagac
     3601 ccgccacgcc gacgccatcg tggcggtggg ctcaggcgca ctgtcggaca cctgcaagta
     3661 tgcgaccttc aaggatgggc gcaaatatgc gaccttcggc acggccgcct cgatgaacgg
     3721 ctatgcggct tccaccgcct cggtcacgct caacaacggc tacaagacct cgctgccggc
     3781 gcatgcgccg cgcggcatct tcctcgatct gcaggtgagc gctgcggcgc cccactggct
     3841 gtcggccgcg ggcctcggcg actcg
//
LOCUS       DBNGVR010000375         2366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1514756, whole genome shotgun sequence.
ACCESSION   DBNGVR010000375 DBNGVR010000000
VERSION     DBNGVR010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1514756"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="AC16A0_07980"
     CDS             <1..488
                     /locus_tag="AC16A0_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3105312.1"
                     /translation="EYTTVEQAVFLAQLKDAIDNKKPIAFYYWTPEWIHAAYKLTKLE
                     EPEFNGYSAESAKGSERYNAEGCYKFYQPSERNDWLAASSIACGQPPTRVNIAHSKAL
                     AERAPQVSQFLKQVTLDGDTVNQWILAMEVDKKPVEQVASEWIAANKDTVEGVWLKGV
                     K"
     gene            543..1616
                     /locus_tag="AC16A0_07985"
     CDS             543..1616
                     /locus_tag="AC16A0_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3105313.1"
                     /translation="MPPLRLAMLGAGNVAESYVRQIRRLREEGFDVELAAICSRSLDP
                     ARRLAETFGIPECGTDIVALLSRKDIDAVIVLTPMQCHAEHVRLALASGKHVLTEKTL
                     AASAEEGRGLTALAAERGLILRAAPFTTLSPVFRDARHRLERGEIGEALCCRALYGWE
                     GPDWADWFFRPGAGALRDLGVYALTTLTGLLGPVASVFALASAVEAGGRGQGLQLSLR
                     FASGCVGTITTGFGLRKFQASGIEIYGSTGTLQFIGQDWDPKGLQLWTTETGCWQMFD
                     QEATWPWTDGVRDFCASIIEETAGDLSLDHALHVLDIIDQALASLDGGMPRPVSSRFE
                     LPRLHPRADGSKAQRNHNPLTLT"
     gene            1637..>2366
                     /locus_tag="AC16A0_07990"
     CDS             1637..>2366
                     /locus_tag="AC16A0_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3105314.1"
                     /translation="MAYKSSPRPTFSEPTLIPYDKVTRYLWGDDEAGLVNDWIYLSSG
                     KIHQLIFGFPAGDGFRHSESFRTIFGADEVLYVLQGNMIIANPETGEVQLVRKGESVF
                     FRKDTWHNAWAMPGEELRVLEYFAPPPSTGTSGKYAQSKPYVSRPRFERRELVGNWPA
                     GRAEAEKRGHFAVLRESDRLWSLSGDHRVLIGIIASTEQLTAGKATIAPGGRSAVIEH
                     GGDAGIYVEAGRLNVQLVDSEQAQT"
BASE COUNT          452 a          768 c          745 g          401 t
ORIGIN      
        1 tggaatacac cactgtggag caggccgttt tcctggccca gctcaaggat gccatcgaca
       61 acaagaagcc catcgccttc tactactgga ctcccgaatg gatccatgcg gcctacaagc
      121 tgaccaaact cgaagaaccc gagttcaacg gatattcggc agagagcgcc aagggaagcg
      181 agcgctacaa tgccgagggc tgctacaagt tttaccaacc gtccgagcgc aatgactggc
      241 tagcggcatc ctcgattgcc tgcggacagc cgccgacccg cgtgaacatc gcgcattcca
      301 aggctctggc ggagcgcgcg ccccaggtct cgcagttcct caagcaggtg accctcgatg
      361 gcgacacggt caatcagtgg atcctcgcca tggaagtcga caagaagccg gttgagcagg
      421 tggccagcga atggatcgcg gccaacaagg ataccgtcga gggcgtctgg ctcaagggcg
      481 tgaagtaacg gaccaggggc cgcgggatgc tttgcccgcg gccccgtttc ggagccgcca
      541 ccatgccacc actcaggctt gccatgctcg gcgccgggaa cgttgccgag agctacgtcc
      601 gtcagatccg gcgcctgcgc gaagaaggtt tcgacgtcga gctggccgcg atctgcagcc
      661 gcagcctaga tccggcccgc cgtctggcag agaccttcgg catcccggaa tgcggaaccg
      721 acatcgtggc actcctctcg cgcaaagaca tcgatgccgt gatcgtgctg acgccgatgc
      781 agtgtcacgc agagcacgtg cgcctggcgc ttgcatcggg aaagcatgtc ttgacggaga
      841 agacacttgc agcgtcggca gaggaggggc gcgggctgac cgcccttgcg gccgaacgcg
      901 ggctgatcct gcgcgccgca cccttcacca ccctcagccc ggtctttcgc gatgcccggc
      961 accggctgga acggggcgaa atcggagagg ctctctgttg ccgtgcgctc tatggctggg
     1021 aaggcccaga ctgggcagac tggttcttcc ggcccggtgc cggcgccctg cgcgacctcg
     1081 gtgtctatgc cttgacgacc ctgaccggat tgctggggcc ggttgcatcc gtgtttgcgc
     1141 tggcaagtgc cgttgaagcg ggcggacgcg ggcaggggct tcagctctcc ctgcgtttcg
     1201 cgtccggttg cgtcggcacg atcaccacgg gtttcggact ccggaagttc caggccagcg
     1261 gaatcgagat ctacggcagc accggaacgc tgcagttcat cggccaggac tgggacccga
     1321 agggacttca actctggacc acggaaaccg gatgctggca gatgttcgac caagaggcga
     1381 catggccctg gaccgatggc gtgcgggatt tctgtgcttc gatcatcgag gagacagccg
     1441 gcgacctcag cctcgaccat gctcttcatg tgctcgacat catcgaccaa gcccttgcat
     1501 cgctcgacgg cggcatgccc aggccggtat ccagccgctt cgagcttccg aggttgcatc
     1561 cgcgtgcgga cggcagcaag gcccaacgca atcacaatcc cctgacactc acctgaacga
     1621 ggacccgtct gacatcatgg cctacaaatc gtcgccacgc cccaccttca gcgaacccac
     1681 cctcatcccc tacgacaagg tcacgcgcta cctgtggggc gacgacgagg cgggacttgt
     1741 caatgactgg atctatctct ccagcggcaa gatccatcag ctgatcttcg gctttccggc
     1801 gggcgacggg tttcgccact ccgaatcctt ccggaccatc ttcggcgcgg acgaagtgct
     1861 ctatgtcctg cagggcaaca tgatcatcgc aaacccggag accggtgagg ttcagctcgt
     1921 gcgcaagggc gagtcggtct tcttccgcaa ggacacatgg cacaatgcct gggccatgcc
     1981 cggcgaggag ctgcgcgtgc tggagtattt cgcgccgccg ccatcgacgg gaacctccgg
     2041 caagtacgcg cagagcaagc cctatgtgag ccggccccgc ttcgaacgcc gcgagcttgt
     2101 gggcaattgg ccggccggcc gtgccgaagc ggagaagcgt ggccacttcg cggtgctgcg
     2161 cgagagcgac cggctgtggt cgctcagcgg cgatcaccgc gtcctgatcg ggatcatcgc
     2221 ctcgaccgag cagctcacag ccggcaaggc gaccatcgcg ccgggcggac gcagtgcggt
     2281 aatcgaacat ggcggcgacg caggcatcta tgtggaggcc gggcgtctca atgttcagct
     2341 cgtcgacagc gagcaggccc agacat
//
LOCUS       DBNGVR010000376         1605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3282836, whole genome shotgun sequence.
ACCESSION   DBNGVR010000376 DBNGVR010000000
VERSION     DBNGVR010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3282836"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="AC16A0_07995"
     CDS             complement(<1..1353)
                     /locus_tag="AC16A0_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR3105315.1"
                     /translation="MTETNPLIVFTPSGKRGRFPQGTPVLQAARALGVDIDSVCGGRA
                     ICGRCQVNVGEGEFAKHGITSRGSHVSEFSKVEERYDKLRGLAAGRRLSCQTRIMGDL
                     VIDVPPESQVHKQVVRKEADTRAIELDPCTRLCFIEVEEPDMHKPSSDLERVYKALED
                     QWQITGLSCDLPIIAELQKVLRKGEWKITAAVFSRAPGGGNRLVAIWPGFHDKAYGIA
                     IDVGSTTIAAHLTNLSTGEVAASAGLMNPQIRFGEDLMSRVSYVMMNPGGETEMTAAI
                     RQALQTLAEEVAGRAGIALNDIMEVVLVGNPVMHHLFLGIDPTELGGAPFALATGLAV
                     TIPTRELDLKLNPGAWAYVLPCIAGHVGADTAGVVLSEAPHEQDEIMLAVDVGTNAEI
                     VLGSKHRLLACSSPTGPAFEGAQISSGQRAAPGAIERIRINPETLEPRYKVIGVDLWS
                     D"
BASE COUNT          264 a          515 c          541 g          285 t
ORIGIN      
        1 gtcagaccac agatcgacac cgatcacctt gtagcggggt tcgagtgtct cggggttgat
       61 gcgaatgcgc tcgatggctc ccggggccgc gcgctggccg gatgaaatct gcgcaccttc
      121 aaaggcaggc ccggtgggcg aggagcaggc cagcaggcgg tgcttcgagc ccagcacgat
      181 ttcggcattg gtgccgacat cgacggccag catgatctcg tcctgctcgt gcggcgcctc
      241 cgacaatacc acgcccgccg tgtcggcccc gacatgcccg gcgatgcagg gcagcacata
      301 ggcccaggcg ccgggattga gcttcaggtc cagctcgcgc gtcggaatgg tgaccgcgag
      361 tccggtggcc agcgcaaagg gcgcgccgcc cagttcggtc ggatcgatgc cgaggaagag
      421 gtgatgcatg accggattgc cgaccagcac cacttccatg atgtcgttga gcgcgatccc
      481 ggcgcgcccg gccacctctt ccgccagcgt ctgcagcgcc tggcggattg cggcggtcat
      541 ttccgtctcg ccgcccggat tcatcatcac ataggagacg cggctcatca gatcctcacc
      601 aaagcggatc tgcggattca tcagccccgc ggaggccgcc acttcaccgg tcgagagatt
      661 ggtgagatgg gcggcaatgg tggtggagcc gacatcgatg gcgatgccat aggccttgtc
      721 gtggaaaccg ggccatatcg ccaccagccg gttgccgccg ccgggcgcgc gcgagaagac
      781 ggcggcggtg atcttccact cgcctttgcg cagaaccttc tgcagctcgg cgatgatcgg
      841 gaggtcgcag gagagcccgg tgatctgcca ctgatcttcc agcgccttgt agacgcgttc
      901 gagatcggaa gagggcttgt gcatgtccgg ctcttcgacc tcgatgaagc acagcctggt
      961 gcagggatcg agctcgatgg cgcgggtatc ggcctccttg cggacgacct gcttgtggac
     1021 ctgcgattcc ggcggcacgt cgatgacgag atcgcccatg atcctggtct gacaggagag
     1081 gcggcgtccg gcggcaaggc cgcgcagctt gtcgtagcgc tcctcgacct tggagaattc
     1141 cgagacatgc gagccgcgcg atgtgatgcc gtgcttggcg aattcgcctt cgcccacgtt
     1201 cacctggcag cgaccgcaga tggcgcgccc gccgcagacc gagtcgatgt cgacgcccag
     1261 cgcccgcgcc gcctgcagca ccggcgtgcc ctgcgggaag cgcccgcgct tgcccgaggg
     1321 cgtgaagacg atgagggggt tggtttcggt catgtcagtg tgaggtcgcg aatggcgaat
     1381 ggcgaatggc gaatggaagg acggagatgg aatgttcggt caagatgctc gcgcgcccca
     1441 ttcgccattc gccactcgct attcgcatct cctatcgcct ccgccgggcc tcgcgcgcct
     1501 tgcgccgggc ttcgatgtcc tcgttcgacg agacttcgcc cgcggcctgc gcggcggcgg
     1561 acggacctgc ggtcatctcg cggaacttct gcgtccagaa cttgc
//
LOCUS       DBNGVR010000377         4035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_928557, whole genome shotgun sequence.
ACCESSION   DBNGVR010000377 DBNGVR010000000
VERSION     DBNGVR010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_928557"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="AC16A0_08000"
     CDS             complement(<1..199)
                     /locus_tag="AC16A0_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003583874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3105316.1"
                     /translation="MTLRPIITLPDEKTLRQISRPVAAVDAEVRKQWDDMLETMYHAP
                     GIGLAAIQIGLPRRLLVIDLAK"
     gene            complement(254..592)
                     /locus_tag="AC16A0_08005"
     CDS             complement(254..592)
                     /locus_tag="AC16A0_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105317.1"
                     /translation="MRLTILGCAAAAALAVLPGAAWAHGNQVPCNPYMIVQTCDADGD
                     NCTARLKYAKGHSLAASPRSSYFYNRPHRWLYHEDFGHKHKVKLSHDGTRKTITYKRK
                     CVPHHHDHEG"
     gene            701..797
                     /gene="ffs"
                     /locus_tag="AC16A0_08010"
     ncRNA           701..797
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16A0_08010"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            824..2539
                     /locus_tag="AC16A0_08015"
     CDS             824..2539
                     /locus_tag="AC16A0_08015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF006585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR3105318.1"
                     /translation="MVDQTSGANASGYRVLARKYRPQTFADLIGQDAMVRTLTNAFAS
                     GRIAQGFMLTGVRGVGKTTTARLIARALNYGDAPTIDMPAFGPHCEAILESRHMDVIE
                     MDAASNTGVDNMREIIDSVRYAPVSARYKVYIIDEVHMLSKSAFNALLKTLEEPPPHV
                     KFIFATTEINKVPVTILSRCQRFDLRRLDADLLATHYAAIAAKEGVEAEADALAMIAR
                     AAEGSVRDGLSLLDQAIAYGEGKVLAGDVAAMLGLMDRSRIIDLFEAVMAGDPARAIM
                     EIEAQHAGGGDPRTILNDLADYVHWVTRIKVVKEPALADTSRTEAEKSRGRDHSAKIG
                     MAHLTRAWSMLLKGIRETELHADPLMAAEMVLIRLAHAADLPGGEELAKLVRSAPPPA
                     EKIVALPPREPLRGGEPQGDAVQAALAVKPQQAVKAAATETRAFSSFRDIIALLTEKR
                     LIKLKADIERFVRPIRVSPGQFELALEPGAHPGLPGEIARNLEAQTGQRWMVMVAKDG
                     GEKPVLQQRQENRDSLFRSAREHPDVQALLKRFPGAEIIDVREPDLPATIDNQTTGET
                     DEEHR"
     gene            2523..2846
                     /locus_tag="AC16A0_08020"
     CDS             2523..2846
                     /locus_tag="AC16A0_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3105319.1"
                     /translation="MKNIGEMMKQVQAMQSRMAEMQAKLEQSTVTGQSGGGMVKVTMT
                     GKGAMTRLEIDPSLMKADEKDILEDLIIAAHSDAKGKSEAMMAEQMKDLTGGLPLPPG
                     LKLPF"
     gene            2846..3454
                     /gene="recR"
                     /locus_tag="AC16A0_08025"
     CDS             2846..3454
                     /gene="recR"
                     /locus_tag="AC16A0_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3105320.1"
                     /translation="MAKRTTGPEIERLIQLLAKLPGLGPRSARRAALHLIKNRERLLA
                     PLAAATAEARDTVRVCSSCGNVDTVDPCTICTDPRRDRSLICVVEEVGDLWALERAGA
                     WPGLYHVLGGTLSAIEGIRPEDLAIAQLIDRATAAQAREVVLALNATVEGQTTAHYIL
                     ERLKQAGIATSRLAHGVPVGGELDYLDEGTLTAAMRARRPMS"
     gene            3580..>4035
                     /locus_tag="AC16A0_08030"
     CDS             3580..>4035
                     /locus_tag="AC16A0_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR3105321.1"
                     /translation="MANLLHTSGIIRRFARSQSGGTAVVFALSLVPLLIAGGVAIDYL
                     RLSEARTELQAALDAGALAVGAAKDLDHDQRILAGTKTFERNMTKAGKDLNAIDYAFE
                     LDGQTVTGTARYDLPTGLMQVVGYVAMGIEADTEITVPDEQKAEVALVLD"
BASE COUNT          716 a         1323 c         1354 g          642 t
ORIGIN      
        1 ccttggccag atcgatcacc agcaggcgtc ttggcaggcc gatctggatg gcagccagcc
       61 cgatgcccgg cgcgtgatac atggtctcga gcatgtcgtc ccactgcttg cgcacctcgg
      121 catcgaccgc cgccacgggc ctggagatct ggcggagcgt cttttcgtcg ggcagggtga
      181 tgatgggccg gagcgtcatg ggcgtcgaga taggcgagcc ggcggccccg gtcaatccgc
      241 cgggagccag gcttcaaccc tcgtggtcat gatgatgcgg gacgcacttg cgcttgtagg
      301 tgatggtctt gcgggtgccg tcatgcgaga gcttcacctt gtgcttgtgg ccgaagtcct
      361 cgtggtagag ccagcggtgc ggcctgttgt agaaatagga acttctgggc gaggcggcga
      421 ggctgtggcc cttggcgtat ttgaggcggg cggtgcaatt gtcgccgtcg gcatcgcagg
      481 tctgaacgat catgtaggga ttgcagggca cctggttgcc atgggcccag gctgcgcccg
      541 gaaggacggc cagcgccgcg gcagctgcac aacccagaat cgtcaggcgc atggatggta
      601 tcctccggct ttgccagcca acagtgacgc ccggcggccg atttggcaag cctccgggca
      661 acccttgcat ttgctccgcc acgggaccat atagccgccg ggaggttggc ggcggacgtg
      721 ccgctcgcca accgggtcag gtccggaagg aagcagcccc aacgagttcg gaacgggttg
      781 cccgtccagc ctcccacctc tattggtacg cccagagccc gccatggttg accagacaag
      841 cggcgcgaac gcttcggggt accgcgttct tgcccgcaaa taccggcccc agaccttcgc
      901 cgacctgatc gggcaggacg cgatggtgcg caccctcacc aatgccttcg cgtcgggacg
      961 catcgcccag ggcttcatgc taaccggtgt gcgcggggtg ggcaagacca ccaccgcccg
     1021 cctcatcgcc cgggcgctga actatggcga tgcccccacc atcgacatgc cggccttcgg
     1081 cccgcactgc gaagccatcc tcgaatcgcg ccacatggac gtgatcgaga tggacgccgc
     1141 ctcaaacacc ggcgtcgaca acatgcgcga gatcatcgac agcgtgcgct atgcgccggt
     1201 ctcggcacgc tacaaggtct acatcatcga cgaagtccac atgctgtcga agagcgcctt
     1261 caacgcgctg ctgaagacgc tggaggaacc tccgccgcac gtgaagttca tcttcgcgac
     1321 tacagagatc aacaaggttc cggtcaccat cctgtcgcgc tgccagcgct tcgacctgcg
     1381 ccgcctggat gccgacctgc tggcgacgca ttatgccgcc atcgccgcga aggaaggcgt
     1441 cgaggccgag gccgatgcgc tggcgatgat cgcgcgggct gccgaaggct cggtccgcga
     1501 cggcctgtcg ctgctggatc aggccatcgc ctatggcgag ggcaaggtcc tggctggcga
     1561 cgtggcagcc atgctgggcc tgatggaccg ctcgcgcatc atcgacctct tcgaggctgt
     1621 gatggcaggc gatccggcca gggccatcat ggagatcgaa gcgcagcatg ccggcggcgg
     1681 cgatccgcgg accatcctca acgacctcgc cgactatgtg cactgggtga cgcgcatcaa
     1741 ggtggtgaag gaaccggcgc tggccgatac ctcgcgcacc gaggccgaga agtcgcgcgg
     1801 gcgcgaccat tcggccaaga tcggcatggc gcatctgacg cgggcctggt cgatgcttct
     1861 caagggcatc cgcgagaccg agcttcatgc cgacccgctg atggcggccg agatggtgct
     1921 gatccgcctc gcccatgcgg ccgatctgcc aggcggcgag gaacttgcga agctggtcag
     1981 gtcggcaccg ccgcctgccg agaagatcgt cgcgctgccg ccgcgcgaac cgctgcgcgg
     2041 cggcgagccg cagggcgacg ccgttcaggc cgcgctggcg gtgaagccgc agcaggccgt
     2101 caaggccgcg gcgaccgaga cccgcgcctt ttcgagcttc agggacatca tcgccctgct
     2161 caccgagaag cggctgatca agctgaaggc cgacatcgag cgcttcgtcc ggccgatccg
     2221 cgtcagtccc ggccagttcg aactggcgct tgaaccgggc gcccatccgg ggcttcccgg
     2281 cgagatcgcg cgcaatctcg aggcccagac cggccagcgc tggatggtga tggtcgccaa
     2341 ggacggcggc gagaagccgg ttctgcagca gcgccaggag aaccgggaca gcctgttccg
     2401 cagcgcgcgc gaacacccgg acgtgcaggc ccttctcaag cggttcccgg gcgccgagat
     2461 catcgacgtg cgcgagccgg acctgccggc aacaatcgac aaccagacaa cgggtgagac
     2521 ggatgaagaa catcggtgag atgatgaagc aggtccaggc catgcagagc cgcatggccg
     2581 agatgcaagc caagctggaa cagtccacgg tgaccggaca gtcgggcggc ggcatggtca
     2641 aggtgaccat gaccggcaag ggcgccatga cccggctgga gatcgatccc tcgctgatga
     2701 aggccgacga gaaggacatt ctcgaggacc tcatcattgc cgcccacagc gatgccaagg
     2761 gcaagtcgga ggcgatgatg gccgagcaga tgaaggatct gacgggcggg ctgcccctgc
     2821 cgccggggct gaaactgccc ttctgatggc gaagcgaacc accggacccg agatcgagcg
     2881 cctgatccag ctgcttgcca agctgccagg cctcggcccg cgttcggccc ggcgcgcggc
     2941 gctgcatctc atcaagaacc gcgagaggct tctggccccg ctggccgccg cgactgccga
     3001 ggcccgcgac acggtcaggg tctgctccag ctgcggcaac gtcgatacgg tcgacccctg
     3061 caccatctgc accgatcccc gccgcgaccg cagcctgatc tgcgtggtcg aggaggtggg
     3121 cgacctctgg gcgctggagc gtgccggggc ctggccgggg ctctaccatg tgctgggcgg
     3181 caccctctcc gcaatcgaag gcatccggcc ggaggatctc gcgattgccc agctgatcga
     3241 cagggcgacg gctgcccagg cgcgggaggt ggttcttgcg ctgaatgcca cggtcgaagg
     3301 gcagaccacc gcccattaca tcctggagcg gctgaagcag gccggcattg ccacctcacg
     3361 cctggcccat ggcgttccgg tgggcggcga gctcgactat ctcgacgagg gcacgctgac
     3421 ggcggccatg cgggcccgcc ggcccatgag ctgagcgcct taaccgtctg ttaacgcaat
     3481 tcgacggtgg cagcccgccg atgcacggtg ggtccggcgc ttgcttcgtg ttcactgtcc
     3541 cttaccggcc tgtgccggtt tgaaacatgg agcaccgaga tggcgaacct gcttcacacc
     3601 tccggcatca tccgccgctt tgcccgcagc cagagcggcg gcacagccgt ggtctttgcc
     3661 ctgagccttg tcccgctgct catcgcaggc ggagtagcaa tcgattatct ccggctgtcg
     3721 gaagcgcgca ccgagttgca ggcggcactc gatgccggcg cgctcgcagt tggcgcggca
     3781 aaggatctcg accacgatca gcgcattctg gccggcacca agaccttcga gcggaacatg
     3841 acgaaggccg gcaaggatct gaatgcgatc gattatgcgt tcgagctgga tggtcagacc
     3901 gtcaccggca ctgctcgcta cgatctgccg accggactga tgcaggtggt gggatatgtg
     3961 gccatgggca tcgaggcgga caccgagatc acggtgcccg atgaacagaa ggccgaagtg
     4021 gccctcgttc tcgac
//
LOCUS       DBNGVR010000378         6254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4656795, whole genome shotgun sequence.
ACCESSION   DBNGVR010000378 DBNGVR010000000
VERSION     DBNGVR010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4656795"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="AC16A0_08035"
     CDS             <1..327
                     /locus_tag="AC16A0_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HIR3105322.1"
                     /translation="MTWVGADVPGGAFQGGTVLAAMWVLVMLAGVRQIPRLSSPALRA
                     AIVAGPVVFLAIGFWGFVSPGQFLAYPQGFAKPLIVAMEIALTLSIGVILGLLIAGPS
                     NGRDAR"
     gene            324..653
                     /locus_tag="AC16A0_08040"
     CDS             324..653
                     /locus_tag="AC16A0_08040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HIR3105323.1"
                     /translation="MSGPMLFWLAGSALVGLGVYGITVNRHPLRKLLALNLLGSGVFL
                     LFGVIARRGAAAGLGGDPVPQALVITGIVVAFSATAIAIALLSQLQSKAGRTSLEDEA
                     ADDEDRS"
     gene            650..2161
                     /locus_tag="AC16A0_08045"
     CDS             650..2161
                     /locus_tag="AC16A0_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HIR3105324.1"
                     /translation="MMPLQSLAAAAPTPDGYLLVLAIILPATAIVALVGLGGRGAAGI
                     VLASLAAQVIIAIRVLVLVWESGAPVAYVVGGWDPPLGIALRADGVSAVMMLTSAVIF
                     SAAAAFAWKEYRPPRGSPESRASLVFWSALLAMASALNTLFASADLFNLYVALELLTF
                     AAIPVVSLKGSAETLRAALRYLIFAVFGSMLYLLGIVLLYGAYGTLDVTSLAALIRPD
                     AVTLVAAALVTVGLLAKTALFPFHLWLPPAHAGAPAAGSAVLSALVVKGSLFLLMRLW
                     FDTFPSLRDPAAMQILAALGAAAVIVGSVMAIRQARLKLLVAYSTVAQIGYMFFVFTL
                     VPVLPGESWIAASAWTGGWLQIVSHAFAKAAMFLAAGLVAEALGHDRIRDLSGLGKRL
                     PLIVLSFAMSGVSLMGLPPSGGFSAKFMLLVAAVAEGRWLWAAVIIGGGLLAGIYVFG
                     VLARMLAAMPDGTRVAEIGRDRQLIALALAGVALLLGFLPLEPMALLQIVRMP"
     gene            2158..3798
                     /locus_tag="AC16A0_08050"
     CDS             2158..3798
                     /locus_tag="AC16A0_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3105325.1"
                     /translation="MSAILVTMVALPLVMLLLLAHRPLRPLIVRALAIAPMPGLLVLP
                     AAWQNGQWSTEFLGTWSLVLDQPAALMLVASSLLWCIGGFAASNWLKARSGQGGFAMW
                     WLMTLAGSLGVFVAGDVASFYLFYAAASLPAYGLIVFMGGTADFKAGRVTLAAALLGE
                     ALILAAFVTLVAGASGQGLLIRDLVSSLAASPYRDPVILLVIVGFGLKIGLVPLHGWM
                     PLSYGAGPLVATAVLSGATSKAGLMGLIRFLPLDAPHPHWGGVLLAAGLLSALYGAVI
                     GLTQSNPRSVLAYSSVSQLGQMAAVLGLGLAAGDSAATVMVAFYAVYHVLTKGGLFLA
                     LGVDQAAHAGAAAKLNLIVIIVLSLGFAGLPLTGGALGKLAVKPIIGDGTASLVFAIA
                     AIGSTLLMLHFIRLQHGVRHDIERAQGNLARSAWFGAAISAMVLPWSLFAAVTAQPLS
                     SAASLNAVVELAWPVAAGAVLWWGILRSGLLIPRLAAGDVWEWAFTWVLVRLQTSANA
                     IAWVEQRSREWAVSAAGMVLVIALLILALTSALAGRIP"
     gene            3905..4255
                     /locus_tag="AC16A0_08055"
     CDS             3905..4255
                     /locus_tag="AC16A0_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105326.1"
                     /translation="MFRVPRLGMIAAGSAVASLLALAAIPAPAEARTNNNWSSVSRSQ
                     QCSREAERYADRRARRRTATTAAGGAAVGGIVGGSRRNAGQGALIGAGAGLFQANSRW
                     RTYYNRAYFRCINR"
     gene            4436..4756
                     /locus_tag="AC16A0_08060"
     CDS             4436..4756
                     /locus_tag="AC16A0_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105327.1"
                     /translation="MSKFNARNVRLSALVLAASAFAAIQMAGNAQAYQCKPGYVQVET
                     IATLKAKSKSSGRKLWTSNVKNSLGLEWSVWDIASNESQDCAFTGAKWYCITKAKPCL
                     YVVQ"
     gene            4931..>6254
                     /locus_tag="AC16A0_08065"
     CDS             4931..>6254
                     /locus_tag="AC16A0_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3105328.1"
                     /translation="MPRLSNILLFEPAPLASLQNRPWSGWLVVGLVCVGAFVGQVDAT
                     IVQLALPTLGHVYDAPLQTISWVALSYLLAYASFLPIFGRLCEILGRKSLYIAGYLLF
                     ILASALCGLAASLEQLLAFRFLQGIGGALLGANSLSILVATVPAESRGRALGLYAAAQ
                     AIGMSAGPALGGFILEALGWRWIFWVTVPFGAAAVILGWLALPRSSAGSAGQAFDWGG
                     MLLVGPALILLVAALNHLSSWGLLSPYTLGCIAAAAALFWLLFRHEAASPSPLINPRL
                     YRTAAYRSGALAVALAYALLYAMFFLMSFALEHGYGETPSHAGLRLALIPVALGLTAP
                     FSHDLKSWLGSSRIAVAGMLLCLCGVTTLLFTLKLSPDHHVYDTLAFILIGTGLGLFI
                     APNFNATIADVTPDLHGPAGSLLNLMRALGTSLGVAAGATTLNWRLEAQ"
BASE COUNT          824 a         2073 c         2005 g         1352 t
ORIGIN      
        1 atgacatggg tcggggccga tgtgccgggt ggtgccttcc agggcggtac cgtgctggcc
       61 gccatgtggg tgcttgtgat gctggccggc gtgcggcaaa tcccgcgcct gtcttcgccg
      121 gccctgcgcg cggcgatcgt ggccgggcct gttgtctttc tggccatcgg cttctggggc
      181 ttcgtctcgc ccgggcagtt cctcgcctat ccgcaaggct ttgccaagcc gcttatcgtc
      241 gcgatggaga ttgccctgac cctgtcgatc ggcgtgatcc tcggcctgct cattgccggg
      301 ccatcgaatg gcagggacgc gcgatgagcg ggcccatgct gttctggctg gccggatccg
      361 ccctcgtagg ccttggcgtc tacggcatca cggtcaatcg gcatccgctc cgcaagctgc
      421 tggcgctcaa cctgctcggg tcgggcgtgt ttctgctctt cggcgtgatc gcacggcgcg
      481 gggctgcggc cggactgggc ggcgatcccg ttccgcaggc gctggtcatc acggggatcg
      541 tcgttgcctt ctctgccacg gcgatcgcca tcgcattgtt gtcgcaactg cagtccaagg
      601 ctggccgaac aagcctggaa gacgaggcag ctgacgacga ggacagatca tgatgccgct
      661 gcagtcgctt gcagccgcgg caccgacgcc cgacggctat ctgctggtgc ttgccatcat
      721 cctgccggcc accgccatcg ttgccctggt cgggctgggc gggagaggcg ctgccggcat
      781 cgtgcttgcc agtcttgccg cacaggtgat catcgcaatt cgggttcttg tccttgtgtg
      841 ggagagcggc gcaccggtcg cctacgttgt tggcggctgg gacccgccgc ttggcatcgc
      901 gctgcgggca gacggcgtct cggccgtcat gatgctgacc agcgcggtca tcttctcagc
      961 tgcagcggcc tttgcctgga aggagtatcg tccgccacgc gggtcgccgg agtcccgggc
     1021 atcgctggtc ttctggtcgg cgctcctcgc catggccagt gcgctcaaca cgctctttgc
     1081 cagcgccgat ctcttcaatc tctatgtcgc cctcgaactc cttaccttcg cggccattcc
     1141 cgtggtgagc ctgaagggca gcgccgaaac cctgcgggcc gctttgcgct acctcatctt
     1201 cgccgtgttt gggtcgatgc tctatcttct cggcatcgtc ctgctctatg gcgcctacgg
     1261 aactctcgac gtcacgtcgc tcgcggcgct gatccggcca gacgccgtga cactggtcgc
     1321 ggccgctctg gtcaccgtgg gcttgctggc gaagacggcg ctgtttccct tccatctctg
     1381 gctgccgccc gcccatgccg gcgcgccggc ggccggcagc gcggtcctct cggctctcgt
     1441 ggtcaaggga agcctcttcc tgctcatgcg cctgtggttc gacacgtttc cgtccctgcg
     1501 cgatccggca gccatgcaga ttctcgccgc gctcggagcg gcggcggtga tcgtcggcag
     1561 cgtgatggcc atacgtcagg cccgcctcaa gctcctggtc gcctattcca ccgtggcgca
     1621 gatcggctac atgttcttcg tcttcacgct cgtgccggtg ctgcctggcg agagctggat
     1681 cgccgcgtcg gcctggaccg gcggctggct tcagatcgtg tcacatgcct tcgcaaaggc
     1741 ggccatgttc cttgcggctg gacttgtggc cgaggccctc gggcatgatc gcatccgcga
     1801 tctgtccggt ctgggcaaac ggcttccgct catcgtcctg agcttcgcca tgagcggcgt
     1861 atcgctgatg ggactcccgc ccagcggcgg attcagcgca aagttcatgt tgcttgtcgc
     1921 ggccgtggca gaggggcgct ggctgtgggc ggccgtgatc atcgggggtg ggctgctcgc
     1981 cggtatctat gtctttggcg tcctcgcacg catgcttgcc gccatgccgg acgggacccg
     2041 ggttgctgag atcggccgtg accggcagtt gatcgccttg gcgcttgccg gcgtggcgct
     2101 tctgctgggg ttcctgcctc ttgagcccat ggcattgctg cagatcgtgc ggatgccatg
     2161 agtgcgatcc tcgtgacgat ggtcgccctg ccactggtca tgctgctgtt gctggcgcat
     2221 cgcccactcc ggccgctgat cgtgagggcg cttgcgattg cacccatgcc cggacttctg
     2281 gtgctgccgg cggcttggca gaatgggcaa tggtcgaccg aatttctcgg gacctggagc
     2341 ctcgtcctcg atcagcccgc cgccctgatg ctcgttgcct cgtccctcct gtggtgcatt
     2401 ggagggtttg ccgccagcaa ctggctgaaa gccaggagcg ggcagggcgg attcgccatg
     2461 tggtggctca tgacgctggc tggcagcctc ggggtcttcg ttgccggtga tgtggcaagc
     2521 ttctatctct tctacgccgc ggcgagcctg ccagcctatg gcctcatcgt ctttatgggc
     2581 ggaacagcgg acttcaaggc cggtcgcgtc accctcgcgg cggctctgct cggcgaggcc
     2641 ctgatccttg cagccttcgt cacgctggtt gccggcgctt cggggcaggg cctccttatc
     2701 cgggatctgg tctcttctct tgccgcgtcg ccgtatcgcg atccggtgat cctgcttgtc
     2761 atcgtgggat tcggtctcaa gatcggactt gtcccgcttc atggatggat gcccttgtcc
     2821 tatggggcag gcccactggt tgccactgcg gtcctgagcg gcgcgacatc gaaggccggg
     2881 ctcatggggc tgatccggtt tcttcctctg gatgcgccgc atccccactg gggcggcgtg
     2941 cttttggctg ccggtctgct ctcggcgctc tatggagcgg tcatcggtct gacccagagc
     3001 aatccccgca gcgttctcgc ctactccagc gtgagccagc ttggtcagat ggctgccgtg
     3061 ctcggtctgg ggctggctgc cggagattcc gctgcgacgg tcatggtggc cttctatgcc
     3121 gtttatcatg tcctgaccaa gggcggactg ttcctggcgc tcggcgtcga tcaagctgcg
     3181 catgcaggcg cggcggcgaa gttgaacctg atcgtgatta tcgtcctttc tcttggcttt
     3241 gccggcttgc ccctgaccgg cggcgcgctg ggcaagctgg ccgtcaagcc gatcatcggc
     3301 gatggcaccg ccagcctcgt cttcgcgatc gccgcaatcg gcagcacgct gctgatgctg
     3361 catttcatcc ggctccagca cggcgtcagg catgacatcg aaagagcgca gggcaatctg
     3421 gcgcgcagcg cctggttcgg cgcggcaatc tcggcaatgg tcttgccatg gtcattgttc
     3481 gcagccgtga cggcgcagcc tctgtcttct gcggcgagcc tgaacgccgt ggtggaactc
     3541 gcatggcctg tggccgcagg cgccgtgctg tggtggggca tcctgcgatc gggcctgctc
     3601 attcctcgtc ttgcagctgg cgatgtctgg gagtgggcct tcacatgggt tctggtacgc
     3661 ttgcaaactt ccgcgaacgc cattgcctgg gttgagcagc gctcgcgtga gtgggccgtt
     3721 tcggccgccg gcatggtcct cgtcattgcg ctgctgatcc ttgccctgac ctctgcgctt
     3781 gccggccgga tcccatgatc cgcaggtcga tgcgcgccgg cagtgccgat ggcgtgaggt
     3841 ctatccaatg gcaggcgact tgcaagtgtg atagacgtac gaaaacagac caaggagttt
     3901 gctgatgttc cgagttccgc gtttgggcat gattgccgca ggaagtgccg tcgccagcct
     3961 gttggctctc gcggcgatac cggcgcccgc cgaagcacgt acgaacaaca attggtcctc
     4021 ggtgtcgcga agccagcaat gctcccgcga ggcggaacgc tacgccgacc ggcgggcccg
     4081 gcgccgcacg gcgacgacgg cggccggcgg cgctgcggtc ggcggcattg tcggcgggag
     4141 ccgccgcaat gccggacagg gtgcgctcat cggagccggc gcaggcctgt tccaggccaa
     4201 cagccgctgg cgcacctact acaatcgcgc ctactttcgg tgcatcaatc gctaggcgac
     4261 ttgcgtctgc aacggcgatc tgctcgcgcc cctgatcccg ggcaaaggcg atcatcgccg
     4321 tgtccctcgg cctgaatccc agatgaacac tgcggccatg cgccgttcag cgcgcatgtc
     4381 gcagagtcct gtcacatcat ccgcaatatc gcgcgatgca tacaggagaa acatcatgtc
     4441 gaagttcaat gcccgaaacg tcaggctctc ggctctcgtg ctcgcagcct cggcctttgc
     4501 cgcaatccag atggccggga acgcgcaggc ctaccagtgc aagcctggct atgtccaggt
     4561 agagaccatc gccacgttga aggccaagtc gaagtcgagc ggccgcaagc tgtggaccag
     4621 caacgtgaag aacagccttg gccttgagtg gtccgtctgg gacattgcca gcaacgagtc
     4681 gcaggactgc gcgtttaccg gcgcgaagtg gtactgcatc acgaaagcca agccctgcct
     4741 ctacgtggtc cagtaactgt cgcggacttg cgaggctgcc ttcgcccgcg tgatgctgca
     4801 gcatgcctga gggcgtgctg cagcatcgct cccgacgacc gctcctgaag acgccgccag
     4861 cgctgcactt gcaagttgcc gatctctccg tctatgcctc gctcttgcaa caatcctgca
     4921 gagcagcgcc atgccacgct tgtcgaacat cctgcttttc gaaccggcgc ccttggcctc
     4981 gctgcagaac cgcccctggt cgggctggct cgttgtgggt ctcgtctgcg ttggcgcctt
     5041 cgtcggccag gtcgatgcca ccatcgtgca actggcgctg cccaccctgg gtcatgtcta
     5101 cgatgcgccg ctccagacca tcagctgggt ggcgctatcc tatcttctgg cttacgcttc
     5161 cttcctgccg atcttcggac ggctctgcga aatcctggga cggaagagcc tctatatcgc
     5221 aggctatttg ctgttcatcc tggccagcgc gctctgcggc ctcgccgcca gtctcgagca
     5281 attgctggcc ttccggtttc tgcagggcat tggcggcgct ctgctgggcg ccaatagtct
     5341 ttccatcctg gtggcgaccg tgccggcgga gtcccgcggc cgggcgctgg gcctttacgc
     5401 ggctgcccag gccatcggca tgagcgccgg tcccgcgctt ggcgggttca tcctcgaggc
     5461 gctcggctgg cgctggatct tctgggtgac cgttccattc ggcgcggccg ctgtgattct
     5521 cggctggctg gcactgccgc gttcgtccgc tggaagcgca ggtcaggctt tcgactgggg
     5581 cgggatgctg ctggtcggtc cggcactgat cctcctggtc gctgccctga accatctctc
     5641 gagctggggc ctgctttcgc catacacgct cggctgcatc gccgcggccg cagccctgtt
     5701 ctggctcctg tttcgccacg aggccgcatc gccgtctccg ctcatcaatc cgcgcctcta
     5761 ccgcaccgcc gcctatcgca gcggcgccct cgcggtggcg cttgcctatg cgctgctcta
     5821 cgccatgttc ttcctgatgt cgttcgcgct tgagcatggc tatggcgaaa cgccctccca
     5881 tgccggcctc cggctggccc tcattcccgt ggcgctcggg ctcacggcgc ccttcagtca
     5941 cgatctgaag tcctggctgg gttcctcgcg catcgccgtt gcaggcatgc tgctgtgcct
     6001 ctgcggcgtg acgacgctgc tcttcacgct gaagctgtct cccgatcatc acgtctatga
     6061 tacgctggcc ttcatcctga tcggtacggg gcttggcctc ttcatcgctc ccaacttcaa
     6121 cgcgacgatc gccgacgtga cgcccgacct gcatgggccg gccggttctt tgctcaacct
     6181 gatgcgggcg cttggcacga gtctcggcgt tgccgcagga gcgacgacgc tgaactggcg
     6241 cctggaggcg caga
//
LOCUS       DBNGVR010000379         3498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3872005, whole genome shotgun sequence.
ACCESSION   DBNGVR010000379 DBNGVR010000000
VERSION     DBNGVR010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3872005"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC16A0_08070"
     CDS             complement(<1..326)
                     /locus_tag="AC16A0_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3105329.1"
                     /translation="MSHLDVHQFICRSDNYGVLVHDHPSGATAAIDAPDADAIEGELK
                     KRGWQLTHIFTTHHHPDHVEGNLELKEKYGCTIIGPRNEAEKIPGIKTQVAGGDGFKW
                     AGREVK"
     gene            394..1170
                     /locus_tag="AC16A0_08075"
     CDS             394..1170
                     /locus_tag="AC16A0_08075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3105330.1"
                     /translation="MDVVDLREFYASPLGNTTRKLIANRLKAKTGSIAAATVLGLGYA
                     MPYLEEAGAEAGTSLSFMMARQGVIHWPAEGPVRSALVDETDLPLLESAVDIIIAVHA
                     LELCHAPAEMLREVWRVLAPQGRLLLVVPNRRGLWARFDTSPFGQGQPFSRPQLAGLL
                     RNCEFSILSWSHALYFPPSGSGMVLSSAGAIESVVGRIMPAVSGVLIVEAVKQVYAIA
                     AGKRVRKLSHRLRPALLPAPSTRTSIDPSRDRLLFPSRPL"
     gene            1226..1996
                     /locus_tag="AC16A0_08080"
     CDS             1226..1996
                     /locus_tag="AC16A0_08080"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR3105331.1"
                     /translation="MNAGPQDPSLDDIRREIDAIDTGLLDLLGQRFRASAKVRSVKAV
                     EGSLAVSPIRPAREAMILRHLVARHPPEVPVDTLVRLWRVILTSSTLAQAAVTVHLPS
                     AVNESPQLRAAVNAHFCATPVSVHADAAAVIARLEARRGDIAVVTPRSSWAAPLMSGK
                     GDGPHVIGILPLLSAGGEPQLLVLGYADAQGTGDDQTLLVGSSSWSGAAPVWQAVAGP
                     SRLVSLPGFLDAADIARDLAPLGWRLAGRYPTPIEVHS"
     gene            1993..3096
                     /gene="hisC"
                     /locus_tag="AC16A0_08085"
     CDS             1993..3096
                     /gene="hisC"
                     /locus_tag="AC16A0_08085"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3105332.1"
                     /translation="MTSSKAELRPQPRPGILDIAAYVPGKSGAKGKKVHKLSSNESPL
                     GASPAAVAAFRDAAGKLELYPDGSAMALREAIAARYGLNPDAIVCGAGSDELLQLIAH
                     AYLGPGDEAIYSEFGFLVYPIAIKSNGATAVVAPERNHTADVDAILARVTPRTRIVFL
                     ANPNNPTGTHLPFSEVKRLHAGLPRDVLLVLDAAYAEYVLRNDYESGIELVSTSNNVI
                     MTRTFSKIHGLAALRLGWAYCPLHVADVLNRIRGPFNVSAPALAAGTAAIADQGFVEQ
                     AIAHNEQWLPWLTRELEALGLKVTPSVGNFLLVHFPQDAKRNAAAADADLAEQGFVLR
                     RMDAYGIPGALRLTVGSEEANRGVVAALKAFLA"
     gene            3093..>3498
                     /locus_tag="AC16A0_08090"
     CDS             3093..>3498
                     /locus_tag="AC16A0_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR3105333.1"
                     /translation="MTALFDRVALVGVGLIGSSLSHAMRSHGLAAHVSAHARSAATRG
                     KVKDLGLAHSVHDTAAAAVVDADLVILCSPVGSYGALAAEIAPALKPGAILTDVGSVK
                     AAVVKDVGPYVPAGVHFIPGHPIAGTEQSGPES"
BASE COUNT          523 a         1177 c         1151 g          647 t
ORIGIN      
        1 atcttcacct cgcgcccggc ccacttgaag ccgtcgccac cggcgacctg cgtcttgatg
       61 ccgggaatct tctcggcttc gttgcgcggg ccgatgatgg tgcagccata cttctccttc
      121 agttcgaggt ttccctcgac gtgatcaggg tgatgatggg tggtgaagat gtgggtgagc
      181 tgccagccgc gtttcttgag ttccccttcg atggcatcgg catcgggggc gtcgatggcg
      241 gccgtggcac ctgacggatg gtcgtgaacc agcacgccgt agttgtcgga ccggcagatg
      301 aactggtgga catcgagatg ggacatgaat cttgctaacc ttggctgaat tggatgatga
      361 ccggggcggc ccctgcggtt atgttgcgcc gccatggacg tggtcgatct ccgcgaattc
      421 tacgcgagcc cgctcggcaa taccacaagg aagctgattg ccaataggct caaggccaag
      481 accggctcca tcgccgcagc gacggtgctg gggcttggct atgccatgcc ctatctggaa
      541 gaagccggag ctgaggccgg aacctcactg tccttcatga tggcccggca gggcgtcatt
      601 cactggccag cggaaggccc agtccgttca gccctggtcg acgagaccga tctgccgctg
      661 ctcgaaagtg cggtcgacat catcattgcc gtgcatgcgc tggaactctg ccatgcgccc
      721 gccgaaatgc tgcgcgaggt ctggcgggtg ctggcgccgc aaggccggct cctgctggtg
      781 gtgcccaacc ggcgcggcct gtgggcccgt ttcgatacct cgcccttcgg ccagggccag
      841 cccttttccc gtccccagct tgcgggcctg ctgcgcaact gcgaattctc catcttgtcc
      901 tggtcgcatg cgctgtattt tccgccctcc ggttccggca tggtgctctc ctcggccggc
      961 gccattgaga gcgtcgtggg ccggatcatg ccggcggtat cgggcgtgct gatcgtcgaa
     1021 gcggtgaagc aggtctatgc catcgcggca ggcaagcggg tgcggaaact gtcgcaccgg
     1081 ttgaggcccg ccctgctgcc ggccccctcg acccggacgt caatcgatcc cagccgggac
     1141 agattgcttt tcccgtcgcg gcccttatga agggcccgcc gtccggctgg cggcccctgg
     1201 cctgccggac cgatcaggaa acgtcatgaa cgccggaccg caggacccat cgctggacga
     1261 cattcgccgc gagatcgacg ccatcgatac ggggctgctc gacctcctcg gccagcgctt
     1321 ccgcgcctcc gccaaggtgc gcagcgtgaa ggcagtggag ggctcgctcg ctgtctcgcc
     1381 catccgtccc gcacgcgagg ccatgatcct gcggcacctg gtcgccaggc atccgccgga
     1441 ggtgccggtc gatacgctgg tgcgtctatg gcgcgtgatc ctgacgtcct ccaccctggc
     1501 ccaggccgcg gtaacggttc acctgccctc ggccgtgaac gaaagccctc agctgcgcgc
     1561 ggcggtgaac gctcatttct gcgccacgcc ggtgtctgtc catgccgatg cagccgccgt
     1621 gatcgcccgg ctcgaagccc gccgcggcga catcgcggtg gtgacgcccc gcagcagctg
     1681 ggccgcgccg ctgatgtccg gcaagggcga tggccctcat gtgatcggca tcttgccgct
     1741 gctctcggcg ggcggtgaac cgcagcttct ggtgctcggc tatgccgatg cccaggggac
     1801 cggcgacgac cagacattgc ttgtcgggtc gtcgagttgg agcggcgccg cgcccgtctg
     1861 gcaggcggtg gccggcccct cgcgactcgt cagccttccg ggcttcctcg acgccgcgga
     1921 catcgcgcgc gaccttgcgc cgcttggatg gcggctcgcc ggtcgctatc cgacacccat
     1981 agaggttcac tcatgacgtc ttccaaagcc gagcttcgcc cgcagccccg cccgggcatt
     2041 ctcgatattg ccgcctatgt gccgggcaag agcggcgcaa agggcaagaa ggtccacaag
     2101 ctctcgtcga acgaatcgcc attgggggcg agccccgccg ccgtggcggc gttccgcgat
     2161 gccgcgggca agctcgaact ctatccggat ggatcggcca tggcgctgcg cgaggccatc
     2221 gcggcgcgct acgggctcaa tcccgatgcc atcgtctgcg gcgcgggctc ggacgaactg
     2281 ttgcagctca ttgcgcatgc ctatctcggc cccggcgacg aggcgatcta ctcggaattc
     2341 ggattcctcg tctatccaat cgccatcaag tcgaatggtg cgaccgccgt ggtggcaccc
     2401 gaacgcaatc acacggccga tgtcgacgcc atcctcgcgc gcgtcacgcc gcgcacgcgc
     2461 atcgtgttcc tggccaaccc gaacaatccg actgggacac atctgccctt cagcgaggtg
     2521 aagcgccttc atgccggcct gccgcgcgac gtgctgctgg ttctggacgc ggcctatgcc
     2581 gaatatgtcc tccgcaacga ctacgagtcg ggcatcgagc tggtgtcgac gtcgaacaat
     2641 gtcatcatga cgcgcacctt ctccaagatt cacgggcttg cggcactccg cctgggctgg
     2701 gcttactgcc ctctgcatgt ggccgatgtg ctgaaccgca tccgcggccc cttcaacgtc
     2761 agcgcgccgg cactggctgc gggcacggcg gcgattgccg accagggctt cgtcgaacag
     2821 gccatcgcgc acaatgagca gtggctgccc tggctcaccc gcgagctgga ggcgctgggc
     2881 ctgaaggtga cgccgagcgt cggcaacttc ctgctggtgc atttcccgca ggatgcgaag
     2941 cgcaatgccg ccgctgccga tgccgatctg gccgagcagg gtttcgtgct gcgccgcatg
     3001 gatgcctatg gcattcccgg cgcgctgcga ctgaccgtgg gcagcgaaga ggccaatcgc
     3061 ggcgtggtgg cggcgctcaa ggcctttctc gcatgaccgc cctgttcgat cgcgtggcgc
     3121 tggtcggcgt gggcctgatc ggctcgtcgc tgagccatgc catgcgcagc catgggcttg
     3181 cggcgcatgt ctcggcgcat gcgcgcagcg ccgccacgcg cggcaaggtg aaggatctgg
     3241 gcctcgcaca cagcgtgcat gatactgctg ctgctgcggt tgtggatgcg gacctggtga
     3301 tcctctgttc gcccgtcggc agctatggcg cgctggcggc cgagatcgcg ccggccctga
     3361 agccgggcgc catcctgacc gatgtcggct cggtgaaggc ggccgtagtg aaggatgtcg
     3421 gcccatatgt tccggcaggc gtgcacttca tcccgggaca tccgattgcg ggaacggagc
     3481 agtccggccc cgagtcgg
//
LOCUS       DBNGVR010000380         2635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1123074, whole genome shotgun sequence.
ACCESSION   DBNGVR010000380 DBNGVR010000000
VERSION     DBNGVR010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1123074"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="AC16A0_08095"
     CDS             <1..230
                     /locus_tag="AC16A0_08095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3105334.1"
                     /translation="AAGLGFEPVLTFGGDYARTLAQWLQRFDAAWPRIADLGFDEKFR
                     RRWRYYLTYCEAGFAERAIDVGIYRFTRPAG"
     gene            271..741
                     /locus_tag="AC16A0_08100"
     CDS             271..741
                     /locus_tag="AC16A0_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 family protein"
                     /protein_id="HIR3105335.1"
                     /translation="MLKRLDPHAGSATFDLLAFLEGETRAEGVFEDRSGRLKRRFTVE
                     TAGRVDGSRLILDESFRFDDGEEMQRTWTLERGANGTFTGTCDDAPLPAQGTLGHDRA
                     AMLSTLLLTVGKRKIAMNFDDVFYPLDSATVLNRSTIRKWGITIGQVLLIFRKT"
     gene            complement(738..>2635)
                     /locus_tag="AC16A0_08105"
     CDS             complement(738..>2635)
                     /locus_tag="AC16A0_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3105336.1"
                     /translation="VNRFRLFADPSFFTLHENAWRQYLLHTDGLDMDADQRAAFVART
                     SVLGSEHVLNDRRIAFSDVLRENFDWRGWAARSEAVLCYDSKSRTHTACGDIDAYLNP
                     TGHEIEMWITTASGVALPAGQECEWRIGGTLVAVEDCAERIRDPRAVLPYPGGAEISV
                     NVTGESPILVDAQVRDLLIAGLGDSFASGEGNPNTPVAFSDVRRHRNLYPERKTNSVA
                     GSATWTDELCHRSLYGHQLRAALQIAIENPKTSVTFLDYSCSGAGGEDGILGPQVYVS
                     RVSDPENSASPGSKLISGGSRDAQLFRLMRELCAEKPEMRKRQLVCPGNRFHRSLDFV
                     FLSIGGNDIGFSNVVAWATLRDGMSASIAGFFGATVSAKDFSDRIRDVLPKTYARLAK
                     AFESSLPLHSANDGVFDPSRIILTAYPDLVTDGRGNICPAPQSDDEQELDFPANQSLN
                     LFSSWLTADGKRLEAVREQFAILYQRMQDLAPDHGWTFAGHVYADGMFEGHGFCARNM
                     DRADDPAELLTIPCWGKPSDGGLACEQRWSGKDGEWVPYNPATQNYPYALRQRWVRTF
                     NDAYMVVNEKVIGRSGQIDERSSEAVFSETTGALHPTAEGHAAMADGLLLSIRPLLQQ
                     MFAEGLR"
BASE COUNT          481 a          828 c          863 g          463 t
ORIGIN      
        1 aggccgccgg gctcggcttt gagccggttc tgaccttcgg cggcgactat gcccgcaccc
       61 tggcgcagtg gcttcagcgg ttcgacgccg cctggccgcg catcgccgac cttggcttcg
      121 acgagaaatt ccgccgccgc tggcgctact acctgaccta ttgcgaggcc ggatttgccg
      181 aacgcgccat cgacgtcggg atctaccgct tcacccggcc ggccggctga cagcggtgat
      241 cggaaaaggc cgcaagcccg tagtgaggtc atgctgaaac gcctcgaccc gcatgccggc
      301 tccgcaacgt tcgacctcct cgccttcctg gaaggggaga cccgggccga aggtgttttc
      361 gaagaccgca gcggccggct gaagcgccgc tttaccgtcg agactgcggg gcgtgtggac
      421 ggaagccggt tgatccttga cgagtccttc cgcttcgacg atggcgagga gatgcagcgc
      481 acctggaccc ttgagcgcgg cgcgaacggt acctttactg gaacctgcga cgatgccccc
      541 ttgccggcgc aggggaccct tggtcacgac cgtgcagcga tgctgtcgac attgctgctg
      601 accgtcggca agcggaagat cgcgatgaac ttcgacgatg tcttctatcc gctcgacagc
      661 gcgaccgtgc tgaaccgctc gaccatccgc aagtggggca tcaccatcgg ccaggtgctg
      721 ctgatctttc gcaagaccta gcgcaagccc tccgcgaaca tctgctgcag aagcggcctg
      781 atgctcagca acagcccgtc agccatggcc gcgtggcctt ctgccgtggg atgcagtgcg
      841 ccggttgtct ccgagaacac cgcctccgaa ctgcgctcgt caatctgccc gctgcggccg
      901 atgacctttt cgttcaccac catgtaggcg tcattgaagg tccgcaccca gcgctgacgc
      961 agggcgtagg gataattctg ggtggcagga ttgtagggca cccattcgcc gtccttgccg
     1021 gaccagcgct gttcgcaggc gagaccgccg tcgctgggct tgccccagca tggaattgtc
     1081 agcagttcgg cgggatcgtc ggcgcggtcc atgttgcgcg cgcagaaacc atgtccttcg
     1141 aacatcccat cggcatagac atgaccggcg aaggtccagc cgtggtcggg cgccagatcc
     1201 tgcatgcgtt ggtagaggat ggcgaactgc tcgcgtacgg cctccagccg cttgccatcg
     1261 gcggtgagcc aggacgagaa gagattgagc gactggttgg ccggaaagtc gagttcctgc
     1321 tcatcgtccg actgcggcgc cgggcagatg ttcccgcgcc catccgtcac caggtcggga
     1381 taggccgtga gaatgatgcg ggacggatcg aaaactccat cattggcgga gtgcagcggc
     1441 agcgaggatt cgaaggcctt ggcaagacgc gcataggtct tgggcaggac gtcgcggatg
     1501 cgatcggaga agtccttggc cgaaaccgtg gcgccgaaga atcccgcaat gctggccgac
     1561 atgccgtcgc ggagcgtcgc ccaggccacc acgttggaaa agccgatgtc attgccgccg
     1621 atcgacagga aaacgaaatc gaggctgcgg tggaacctgt tgccaggaca caccagctgc
     1681 cgcttgcgca tctctggctt ctcggcgcag agctcgcgca tcagccggaa gagctgcgca
     1741 tcgcggcttc cgccggaaat gagcttcgac ccgggcgagg cggaattctc aggatcgctg
     1801 actcgcgaca catagacctg cggcccgagg atgccgtcct cgccgccggc gccggaacag
     1861 gaatagtcaa ggaaagtgac ggaagtcttg gggttctcga tcgcaatctg caatgcggcc
     1921 cgcagctgat ggccataaag cgagcggtga cagagttcgt cggtccaggt ggcggagcca
     1981 gccacgctgt tggtcttgcg ttccggatag agattgcggt ggcgccgcac gtcactgaac
     2041 gcgaccggcg tgttgggatt gccctcgccc gaggcgaaac tgtcacccag tccggcgatc
     2101 agcagatcgc ggacttgcgc atccacaaga atgggagact cgcccgtcac attcacggag
     2161 atctcggccc cgcccggata gggcagcaca gcgcggggat cgcgaatccg ctcggcgcaa
     2221 tcctccacgg ccaccagtgt tccgccgatc cgccattcgc attcctggcc tgcgggcagg
     2281 gccacgcccg aagcggtggt gatccacatt tcgatctcgt ggccggtggg gttcagatag
     2341 gcgtcgatgt cgccgcaggc cgtatgggtt ctcgacttgg agtcgtagca gagaacggcc
     2401 tcgctgcggg ccgcccatcc gcgccagtcg aagttctcgc ggaggacgtc gctgaaagcg
     2461 atgcggcggt cgttcagcac atgctccgag cccagcaccg aggtgcgtgc cacgaaggcg
     2521 gcacgctggt ctgcgtccat gtcgaggcca tcggtgtgca gcagatattg ccgccaggcg
     2581 ttctcgtgga gcgtgaagaa gctagggtct gcaaagaggc gaaagcggtt cacca
//
LOCUS       DBNGVR010000381         7224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4856340, whole genome shotgun sequence.
ACCESSION   DBNGVR010000381 DBNGVR010000000
VERSION     DBNGVR010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4856340"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="trpD"
                     /locus_tag="AC16A0_08110"
     CDS             <1..665
                     /gene="trpD"
                     /locus_tag="AC16A0_08110"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3105337.1"
                     /translation="KSGAADVLMALGVKIDLAPERISSCIEQAGLGFMFAPAHHASMK
                     HVGPSRVELGTRTIFNLLGPLSNPAGVKRQVTGVFSKAWVEPLAQVLKNLGSESCWIC
                     HGEGGFDEIVPSGTTWVSALSDGKITSFEITPESVGLKRSKVEDLKGGEAAHNAEALR
                     AVLRGEAHAFRDAAVMTSAAALVVAGRTKDLMSGVAAAQAAIDSGAALRALDTLVKVS
                     NG"
     gene            665..1459
                     /gene="trpC"
                     /locus_tag="AC16A0_08115"
     CDS             665..1459
                     /gene="trpC"
                     /locus_tag="AC16A0_08115"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR3105338.1"
                     /translation="MTTILDKIAAYKREEVAKAKAEIPERELEVMALDAPPVRSFSGA
                     LRSKAETGQWALIAEIKKASPSKGLIRADFNPPQLAMAYEDGGAACLSVLTDTPSFQG
                     APEYLGAARAVTQLPALRKDFMVDTYQVAEARAWGADCILIIMAMIDDGLAGELEAAA
                     HDWGLDVLAEVHDEAELERALKLSTPLIGINNRNLHTFETSLETTERLAPLVPGERMV
                     IGESGLFTPADLARLSRVGVNSFLIGESLMRQTDVASATRAILAKP"
     gene            1456..1932
                     /gene="moaC"
                     /locus_tag="AC16A0_08120"
     CDS             1456..1932
                     /gene="moaC"
                     /locus_tag="AC16A0_08120"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR3105339.1"
                     /translation="MSKLTHIDSAGHAHMVDVSGKETTDRSATAEAVIHMKPETLALI
                     IEGKAKKGDVFATARIAGIMAAKRTHELIPLCHPLMLSKVSIDFAPDTHAAAIKVSAM
                     VKVEGKTGVEMEALTACSVACLTLYDMCKAVDRGMTITDFRLVEKSGGKSGHYKAE"
     gene            1936..3141
                     /locus_tag="AC16A0_08125"
     CDS             1936..3141
                     /locus_tag="AC16A0_08125"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3105340.1"
                     /translation="MALMPVEDARARILQGARRCAAESVRLADGLGRVLATPVKAIRD
                     QPPFEASAMDGYAVKASDVVAVPALLRVIGISAAGHGFKGHVTSGEAVRIFTGAPLPK
                     GTDAVVIQENTAVSAPSQVTILHPVREAQNVRSRGLDFKRGETLLTAGTRLNARDIGL
                     AGAANAGQVRVVRKPMVAVFATGDELVAPGGSPEPDQIVSSNSQALVALVRHFGGDPL
                     DLGIVPDKLKATERTIARAAGADILVTTGGASVGDHDFVQQALKNAGVRIDFWKIAMR
                     PGKPFMYGRKGRQHVMGLPGNPVSALVCARLFLKPLIDALLGLPAGDDLVLARLGAPV
                     KANDDRQDYVRATLRTEPDGTRIATPYMKQDSSMQRTFQQSDCLIVRPPGAPEAATEL
                     LVPILKIDF"
     gene            3242..3946
                     /gene="lexA"
                     /locus_tag="AC16A0_08130"
     CDS             3242..3946
                     /gene="lexA"
                     /locus_tag="AC16A0_08130"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR3105341.1"
                     /translation="MLTRKQLELLMFINERLKEEGVPPSFEEMKEALNLQSKSGVHRL
                     IVALEERGFLRRMPNRARALEVLKLPESHSPSLTPRKQSFTPSVIQGNLGRVKPVASS
                     NDGAPRAAVVPMMGRIAAGVPISAIQEHSANITVPPELLGGGEHFALEVKGDSMIDAG
                     IFDGDTVIIRKGDTASNGDIIVALVDDEEATLKRLRRKGDSVALEAANPTYETRIFGP
                     DRIRVQGRLVGLLRKY"
     gene            4009..4620
                     /locus_tag="AC16A0_08135"
     CDS             4009..4620
                     /locus_tag="AC16A0_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3105342.1"
                     /translation="MRLYGDMLSPFVRMCFVTAHEVGLGDKVIHERESVTPTAPNPRL
                     VALSPIGKVPVLETDHGHAVYDSRVIMEYLCHVAGNKALIPDEGVKRFRVLTLQALGL
                     GLGDAAVGLRYETAARPAAFHWPEWSARLKARIVAACDELESKWGSSLAEVNAGSIAV
                     GVVLSYVGYRLPDLRWQDGRPQLAAFHAGFANRDSMLRTPIGA"
     gene            complement(4621..5001)
                     /locus_tag="AC16A0_08140"
     CDS             complement(4621..5001)
                     /locus_tag="AC16A0_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3105343.1"
                     /translation="MAKHYEGGCLCGSIRFIAEGPPLRPHTCSCKMCQRHSGALTASW
                     VEFPRDMVAWTGPGGTPKTWRSSEFSSRAFCADCGSSLGAIDDKPVIALLIGTFDSSI
                     RKELKPEYHSYRGGRPRWWHIEVD"
     gene            5109..6314
                     /locus_tag="AC16A0_08145"
     CDS             5109..6314
                     /locus_tag="AC16A0_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3105344.1"
                     /translation="MSEYECLVAGAGPAGLAAACLLALDGRRTGLVAAASDAADPRTV
                     ALMRPSIRLLEHLGLWPGELRERTQPLRRLRLVDDTGAAISAPTITFDPDEIGEEAFG
                     WNFPLSILIPQLKARAVQLGVDMITEDAVRADPSSSQVAIVTTGGRVLTSQVVLAADG
                     RNSVLRDAAGIKTNGWSYDQVAVATSFSHSGPHDGISTEYHKGAGPFTTVPLPGHASS
                     LVWMERPQRAAEIMAMPDGQLAREIQLQTHGELARIGDIGPRRAFPMRGLVARDFARH
                     RILLIGESAHVVPPIGAQGLNMSLRDAAQAAELIALEDDPGHASILVQYDRLRRRDVQ
                     PRQQVIDIMNRSLLSGHVLLEGGRALGLSAIAAFGPLRRFVMAQGLAPVSTVPRVMRA
                     QGKMPVLMK"
     gene            complement(6284..>7224)
                     /locus_tag="AC16A0_08150"
     CDS             complement(6284..>7224)
                     /locus_tag="AC16A0_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3105345.1"
                     /translation="NLAMPFFGLILLGVLASRVWKLPDDGLAWLNIFLVYFALPALIF
                     LVVAAAPFEQLVNWPFVSAVTAVTMTAFVVVTGASMWLFKTPLKIAALQGTSGSYGNV
                     GYMGLPLSVAFFGPEAAVPAALVFCFDCTVQFVLTAFLATLAHERNEEAHWGAVAWRI
                     VKQVATHPFIVATALGVAASALQFRAPGALGTILEMLMRSAGPTALFALGVTVGMRRF
                     AGIGRELPLVAGMKVFVQPALALLVVGMVPGIDPTWLHVAVMMAALPTASNAFILASQ
                     YKAYVEGSSTAVIVTTALSAITIPAIIYFIKTGILP"
BASE COUNT         1375 a         2296 c         2272 g         1281 t
ORIGIN      
        1 cgaaatccgg ggctgcggac gtgctcatgg cgctcggcgt gaagatcgac ctcgcgccgg
       61 agcggatatc cagctgcatt gagcaggccg ggctgggctt catgttcgcc cccgcgcatc
      121 acgcctcgat gaagcacgtc ggcccgtccc gcgtcgagct cggcacgcgc acgatcttca
      181 atctgcttgg ccccctctcc aatccggcag gcgtcaagcg tcaggtgacg ggcgtgtttt
      241 cgaaggcctg ggtcgagccg ctggcgcagg ttctgaagaa cctcggaagc gagtcctgct
      301 ggatctgcca tggtgagggt ggatttgacg agatcgttcc ctccggcacg acgtgggtct
      361 ctgccctgag cgacggcaag atcacaagct ttgaaatcac cccggagtcg gtcggactga
      421 agcggtcgaa agtcgaagac ctcaagggcg gcgaggctgc tcataacgcc gaggcgcttc
      481 gtgccgtgct gcgcggcgag gcccatgcct tccgcgatgc ggcggtgatg acatctgccg
      541 cggcacttgt ggttgctgga aggaccaagg atcttatgtc cggcgttgct gccgcgcagg
      601 ctgccatcga cagtggcgct gccctgaggg cactggatac actggtgaag gtttcgaacg
      661 gctgatgaca accatcctcg acaagatcgc agcctacaag cgcgaggaag tggccaaggc
      721 caaggccgaa attcccgagc gggaactcga ggtcatggcg ctggatgccc cgcctgtgag
      781 gagcttctcc ggtgccctga gatccaaggc cgagactggc caatgggcct tgatcgccga
      841 gatcaagaaa gccagcccct cgaagggcct gatccgcgcc gacttcaatc caccccaact
      901 ggcgatggcc tatgaggacg gcggtgccgc ctgcctctct gtgctgaccg ataccccgtc
      961 ctttcagggc gctcccgagt atcttggggc agcgcgggcc gtgacgcagc ttcccgccct
     1021 gcgcaaggac ttcatggtcg atacctacca ggttgcagag gcccgcgcct ggggcgccga
     1081 ctgcatcctc atcatcatgg ccatgatcga cgatggcctt gccggtgagt tggaggcagc
     1141 agcgcacgac tggggcctgg acgtcctcgc ggaagtccat gacgaagccg agctggagcg
     1201 ggccctgaaa ctctccacgc cgctgatcgg catcaacaat cggaacctcc ataccttcga
     1261 gacctcgctg gaaacgacag agcggctggc gccactggtg cctggagaga ggatggtcat
     1321 aggcgaaagc ggcctcttca caccggccga cctggcacgc ctgtcccgcg tcggcgtgaa
     1381 cagcttcctg atcggcgaga gcctgatgcg gcagacggat gtggcatctg caacccgtgc
     1441 gatcctggcc aagccatgag caaactgacc cacatcgact cggccggcca tgcgcatatg
     1501 gtggatgtgt cgggcaagga gacaaccgac cgtagcgcca cagccgaagc cgtcattcac
     1561 atgaagcccg aaacactcgc gctgatcatc gaaggcaagg ccaagaaggg cgatgtcttc
     1621 gccacggcgc ggattgcggg gatcatggcc gccaagcgta cccacgagct catcccgctg
     1681 tgtcaccccc tgatgctctc caaggtctcc atcgacttcg cccccgatac ccatgccgcc
     1741 gcgatcaagg tgtccgcgat ggtgaaggtt gagggcaaga ccggcgttga gatggaggcg
     1801 ctgacggcct gttccgtcgc ctgcttgacg ctctacgaca tgtgcaaggc ggttgaccgc
     1861 ggcatgacga tcaccgactt ccggctggtc gaaaaaagcg gcggcaagtc cggtcactac
     1921 aaagcggagt aagtcatggc cctgatgccg gttgaagacg cccgcgcgcg gatccttcaa
     1981 ggggcgcggc gctgcgctgc tgagagcgtg cgccttgcgg acgggcttgg acgggtactg
     2041 gcaactcccg tgaaggccat ccgcgatcag ccacccttcg aggcttcggc gatggacggc
     2101 tacgcggtca aggcaagcga tgtcgtggcc gtgccagctt tgctgagggt tatcggaatc
     2161 agtgcagcgg gtcacggctt caagggacac gtcacgtccg gagaggccgt ccggatcttt
     2221 accggagcgc ccctgcccaa ggggacggac gcggtcgtga ttcaggagaa caccgccgtc
     2281 tctgccccgt ctcaggtgac gatccttcat ccggttcgcg aggctcagaa tgtccggagc
     2341 cgcggcctcg acttcaagcg cggcgagacg ctgctcacag cggggacgcg tctcaatgcg
     2401 agggacatcg gtctcgccgg cgctgcgaat gccggacagg tgcgggtggt gcgcaaaccc
     2461 atggtcgcgg tcttcgcaac cggggatgaa ctggtggctc ccggaggaag ccccgagccc
     2521 gaccagatcg tctcctccaa cagccaggcg ctcgttgcgc tggtccgaca tttcggaggc
     2581 gatccgcttg atctcggcat tgtcccggac aagctgaagg cgaccgagcg gaccatagcc
     2641 cgggcggcgg gagccgacat cctggtcacg acgggtggcg cttccgtcgg cgaccatgac
     2701 ttcgttcagc aagccttgaa gaatgccggg gtcaggattg acttctggaa gattgccatg
     2761 cggccgggaa agcccttcat gtatggccga aagggccgcc agcacgtgat gggtctgccg
     2821 ggcaaccctg tctcggcctt ggtctgtgca aggctcttcc tcaagccgtt gatcgacgcc
     2881 ctgttgggtt tgccggccgg cgatgatctg gtcttggccc gtctgggcgc gcccgtgaag
     2941 gcaaacgatg accgccagga ctatgtgcgg gccacacttc ggaccgaacc tgatgggacg
     3001 cgcattgcga ctccctacat gaaacaggac tcctcgatgc agcgcacatt tcagcagtcg
     3061 gactgcctga tcgtgagacc tcccggtgcg cccgaagcag ctacggagct attggttccc
     3121 attctgaaga tcgattttta accgcgacag aaagtaaaca cttgaagaac taaatggcaa
     3181 cctgtaagct tgtttcaggt ttgttccgat tcaggaaccc ggcaagacag gacttagcca
     3241 tatgctgacg cgcaagcagc tcgaactcct catgttcatc aacgaacgcc tgaaggaaga
     3301 aggcgtcccg ccgtctttcg aggagatgaa ggaggccctc aatcttcagt ccaagtccgg
     3361 cgtccatcgc ctgatcgtgg cccttgagga acgcggcttc ctgcggcgca tgccgaaccg
     3421 ggcgcgggca ctcgaggtgc tgaagctgcc agaaagtcat tcgccaagcc tcacgccgcg
     3481 caaacagtcc ttcacgccca gcgtcatcca gggcaatctc gggcgggtaa agccggttgc
     3541 ctccagcaac gacggtgcgc cccgtgccgc ggtggtgccc atgatgggcc ggatcgctgc
     3601 gggcgtgccg atctcggcca tacaggagca ctccgcaaac atcaccgtgc cgcccgaact
     3661 gctcgggggc ggcgaacatt tcgctcttga agtgaaaggt gactcaatga tcgacgccgg
     3721 gattttcgat ggcgatacgg tgatcatccg caagggcgat acggcttcca acggcgacat
     3781 catcgtggcc cttgtcgacg acgaggaagc gacgctgaag cgcctgcgcc gcaagggaga
     3841 ctcggtggct ctcgaggcgg caaatccgac ctatgagacc cgcatcttcg gtcccgaccg
     3901 cattcgcgtg cagggccgcc tggtaggact tctccgcaaa tattgatgct tctggccgcg
     3961 cggtgtttgc gttaggttcg gtctaagctg ttttggagag agcgtgcgat gagactctat
     4021 ggcgatatgc tgtcaccttt cgtgcgcatg tgcttcgtca cggcccatga ggtgggactt
     4081 ggcgataaag tcatccacga aagggagtcg gtcacgccga ccgcaccaaa tccccgcctc
     4141 gttgccctct ctccgatcgg caaggttcct gttcttgaaa ccgatcacgg ccatgccgtt
     4201 tatgattccc gcgtcatcat ggagtacctc tgccatgtgg ccggcaacaa agcgctcatc
     4261 cctgacgaag gcgtcaagcg ctttcgcgtc ctgaccctgc aggcgctggg gctggggctc
     4321 ggtgacgccg cggtcggact gcggtacgag acggctgccc gtcccgccgc atttcactgg
     4381 ccggagtggt ctgcacgcct caaggcccgt atcgtggcgg catgtgacga actcgagagc
     4441 aagtgggggt ccagtcttgc ggaggtcaat gccgggtcga tcgcggtcgg cgtagtactg
     4501 tcctatgtgg gctaccgtct ccccgatctc cgctggcaag acgggcggcc gcaacttgca
     4561 gccttccatg caggcttcgc gaaccgcgac agcatgctga ggaccccgat tggcgcgtag
     4621 ctagtcgacc tcgatgtgcc accagcgggg ccggcctcca cgataggagt ggtactctgg
     4681 cttgagctcc ttccgaatgc tggagtcgaa ggtgccaatc agcagggcga tgacaggctt
     4741 gtcatcgatg gcgcccaggc tgctgccaca atctgcacag aaggcgcggc tcgagaattc
     4801 cgaggatctc caggttttcg gcgtgccgcc cgggccggtc caggcaacca tgtcacgcgg
     4861 aaactcgacc cagctggcgg tcagggcgcc cgaatggcgc tggcacatct tgcaggaaca
     4921 ggtatggggc cgcaggggtg ggccttccgc aatgaagcga atggatccgc acagacagcc
     4981 gccttcgtag tgtttcgcca tgttttcctt cccaatgacg tgtgtacgcc cgatccgcgg
     5041 tcaaagatca atctgcccgc gacaagccaa cacttgcatc accgccttga agtctccagt
     5101 ttcctgtcat gagcgaatac gagtgccttg ttgccggcgc gggaccggct ggacttgcag
     5161 cagcctgcct cctcgccctg gacgggcgga ggaccggtct cgtggccgca gcttcagatg
     5221 ccgccgatcc gcggacggta gccctgatgc gcccctctat ccgccttctc gagcatctcg
     5281 gcctctggcc gggcgaactt cgcgaaagga cccaacccct ccggcgcctg cgccttgtgg
     5341 acgataccgg cgcggccatc tccgccccga ccatcacctt cgatccggac gaaatcggcg
     5401 aagaggcctt cggctggaac ttccccctca gcatactcat tccccagctg aaagcacggg
     5461 cggttcagct tggcgtcgat atgatcaccg aagatgccgt gcgagccgat ccttcatctt
     5521 cgcaagtcgc catcgtcacg acaggtggcc gcgtgctcac cagccaggtc gtgctggcag
     5581 ccgatggccg gaattcggtc ttgcgcgatg cggcaggcat caagacgaat ggctggagct
     5641 atgaccaggt cgccgtggcg acgagttttt cgcactctgg tcctcacgat ggcatctcga
     5701 ccgagtatca caaaggcgcc ggccccttca ccacggtgcc cctgcccggc cacgcctcga
     5761 gcctggtctg gatggagcgc ccgcagcgcg ctgccgaaat catggccatg ccggatggcc
     5821 agctggcgcg ggagatccag ctgcagaccc atggcgaact cgcgcgcatc ggcgacatcg
     5881 gtccacgccg ggccttcccg atgcgcggcc ttgtggcacg ggacttcgcc agacaccgca
     5941 tcctcctgat cggcgagtca gcccatgtgg ttccgcccat tggcgcgcag ggcctcaaca
     6001 tgtccctccg ggacgccgca caggcggcgg aactcatcgc tctcgaagac gatcccggtc
     6061 acgcttccat cctggtgcag tatgacaggc tgcgccgtcg cgacgtgcag ccccgccagc
     6121 aggtcatcga catcatgaac cgctccctgc tgtccggaca cgtactgctt gaaggcgggc
     6181 gtgcgctggg cctctcggcg attgcggcct tcggtccgct acgccgcttc gtcatggccc
     6241 aggggctcgc gccggtgtca acagtcccgc gcgtcatgcg cgctcagggc aagatgccgg
     6301 tcttgatgaa gtagatgatc gccggaatgg taatcgccga cagtgccgtc gtcacgatga
     6361 ccgcggtcga cgaaccttcg acataggcct tgtactggct ggccagaatg aaggcattgg
     6421 atgcggtcgg gagggccgcc atcatcacgg caacatggag ccaggtcgga tcgatccccg
     6481 gcaccatgcc gacgaccagc agcgcaagcg ccggctgcac gaagaccttc atgccggcaa
     6541 ccagcggcaa ctcgcgcccg atgcctgcga agcgccgcat gcccaccgtg acaccgaggg
     6601 caaagagggc cgtcgggcct gccgaccgca tcagcatttc gaggatcgtt cccagggcgc
     6661 caggtgccct gaactgcagg gccgaggcag caacgcccag ggcggttgcc acgatgaagg
     6721 gatgggtcgc cacctgtttc acgatgcgcc aggcgacggc tccccaatgc gcttcctcgt
     6781 tccgctcatg ggcaagggtc gcaaggaaag cggtaagcac gaactggacc gtacagtcga
     6841 aacagaaaac cagcgctgcc ggcactgcag cctcgggacc aaagaaggcc accgaaagcg
     6901 gcaaccccat gtagccgaca tttccgtagc ttcccgacgt tccctgcaac gccgcgatct
     6961 tcagcggggt cttgaacagc cacattgaag ccccggtcac cacgacgaag gctgtcatgg
     7021 tgacagcagt gaccgcagac acgaagggcc agttgaccag ctgttcgaag ggcgctgctg
     7081 ccacaacgag gaagatcagg gctggaagcg cgaagtagac cagaaagatg ttgagccacg
     7141 ccagcccgtc atccggcagt ttccatacgc gggaggccaa aacccccaga aggatcagcc
     7201 cgaagaaggg catggcaaga ttga
//
LOCUS       DBNGVR010000382         1923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4070816, whole genome shotgun sequence.
ACCESSION   DBNGVR010000382 DBNGVR010000000
VERSION     DBNGVR010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4070816"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="AC16A0_08155"
     CDS             complement(<1..300)
                     /locus_tag="AC16A0_08155"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3105346.1"
                     /translation="MSTIWNHDPETGELIEEAKANLGEFSVTEIAHALKRTLEDRFGH
                     VRVRGEISGYRGPQSSGHCYFAITDEGAKLDAVIWRGSFGRLRAKLQEGLEVIATG"
     gene            368..1642
                     /gene="purD"
                     /locus_tag="AC16A0_08160"
     CDS             368..1642
                     /gene="purD"
                     /locus_tag="AC16A0_08160"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3105347.1"
                     /translation="MNILLIGSGGREHALAWAISASPLCDKLFCAPGNPGIDQVARVV
                     DLKIADHGAIIAFCKAEAIGLVVVGPEAPLVAGLSDDLRAAGIKVFGPSKFAAQLEGS
                     KGFTKDICAKHGIPTAAYGRFSDRDKALAYLETHGAPIVIKADGLAAGKGVTVAMTMA
                     EARAAVADIFSGKFGGAECVVEEFLEGEEASFFVLTDGKNALPLTTAQDHKRVGDGDT
                     GPNTGGMGAYSPAPCMTPALCNEALERIVKPTIRALADMGHPYLGVLYAGLILTRDGP
                     KLIEYNARFGDPECQVLMMRLKDDLVALLLACCDGTLDRMSVRWFDEAALTIVLCAKG
                     YPGEVVKGSAIRGLEGAGSLEGVEIFHAGTALKNGELVANGGRVLNVTAIGKTVREAQ
                     ARAYAAVDRIDWPEGFCRHDIGWRAIAREEDR"
     gene            1731..>1923
                     /locus_tag="AC16A0_08165"
     CDS             1731..>1923
                     /locus_tag="AC16A0_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3105348.1"
                     /translation="MNQLFPATREHRLRIGDADIFARIGGHGPPLLLIHGFPQTHAMW
                     HRIAPDLMKRFTCVMPDLRG"
BASE COUNT          348 a          638 c          603 g          334 t
ORIGIN      
        1 gccggttgcg atgacctcga gaccctcctg caacttggcg cgcagccggc caaagctgcc
       61 gcgccagatc actgcgtcca gctttgcccc ctcgtccgtg atcgcaaaat agcaatggcc
      121 cgaggactgc ggcccgcgat agcctgaaat ctcgcccctg acccggacat ggccgaagcg
      181 gtcctccagc gtccgcttga gcgcgtgggc gatctcggtg accgagaact cgccgagatt
      241 ggccttggcc tcctcgatga gctcgccagt ctccggatcg tgattccaga tggtggacat
      301 tgcaattcta ccctttgatt ccgggacatc aaatgcgaga aggcggcgga aagcaaggac
      361 gccacccatg aacatcctcc tgatcggctc cggcggccgc gaacacgccc tggcctgggc
      421 catctccgcc agccccttgt gcgacaagct cttctgcgcg ccgggaaatc ccggcatcga
      481 ccaggtcgca agggtggtcg atctcaagat tgctgatcat ggggccatca tcgccttctg
      541 caaggcagag gcgatcggcc tcgtggtcgt gggtcccgaa gcgccgcttg tggccggact
      601 gtcggacgac cttcgtgcgg cgggcatcaa ggttttcggc ccgtcgaaat tcgccgccca
      661 gcttgaaggc tcgaagggct tcaccaagga catctgcgcc aagcatggca ttccgacggc
      721 cgcctacggt cgcttcagcg acagggacaa ggcgcttgcc tatctagaga cccacggcgc
      781 acccatcgtc atcaaggccg acggtcttgc cgcaggcaag ggcgtgaccg tcgccatgac
      841 aatggcggaa gcccgcgccg ccgtcgcgga catcttcagc ggcaagttcg gcggggccga
      901 atgcgtggtc gaggaattcc tcgagggcga ggaggccagc ttctttgtgc tgacggatgg
      961 aaagaacgca ttgccgctca ccacagcaca ggatcacaag cgcgtcggcg atggcgacac
     1021 cggacccaat accggcggca tgggtgccta ctcgccggcg ccctgcatga cgcccgcgct
     1081 gtgcaacgag gcgctggagc gcatcgtcaa accgaccatc cgcgcgcttg ccgacatggg
     1141 ccatccctac ctgggcgtac tctacgccgg attgatcctc acccgggacg gtcccaagct
     1201 catcgagtac aacgcccgct tcggagatcc cgaatgccag gtgctgatga tgcggctgaa
     1261 ggacgacctc gtggcgctgc tgctggcctg ttgcgatggg acactcgacc gcatgtcggt
     1321 gcgctggttc gacgaggcgg cactgaccat cgttctctgc gccaagggtt atccgggcga
     1381 ggtcgtcaag ggttcggcca tccgcggtct cgagggagca ggcagcctgg agggcgtcga
     1441 gatcttccat gccggcaccg ccctgaaaaa tggagaactc gtcgccaatg gcgggcgggt
     1501 gctcaacgtc acggccatcg gcaagacggt gcgcgaggcc caggcccggg cctatgcagc
     1561 ggtcgaccgt atcgactggc ccgagggttt ctgccgacac gacatcggct ggcgcgccat
     1621 cgcgcgggaa gaagacaggt gatgagcatg gccgctcggc ccggatgccg ccagcacgag
     1681 caattgccga ggacttggcg cagctcaccg ccggggtgta acctcccgcc atgaaccagc
     1741 tgttccccgc cacccgcgaa cataggttgc gcatcggcga cgccgatatc tttgcccgca
     1801 tcggcggcca tggccctccc ttgctcctca ttcatggatt tccgcaaacc catgccatgt
     1861 ggcaccggat cgcgcctgac cttatgaagc gcttcacctg cgtgatgccc gatcttcggg
     1921 gtt
//
LOCUS       DBNGVR010000383         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2106572, whole genome shotgun sequence.
ACCESSION   DBNGVR010000383 DBNGVR010000000
VERSION     DBNGVR010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2106572"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16A0_08170"
     CDS             <1..482
                     /locus_tag="AC16A0_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008531626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3095 family protein"
                     /protein_id="HIR3105349.1"
                     /translation="EEERGGHPIPEVGPETSFKPGNTTYETRAGGAPLLRPLRRLGII
                     AQVWLVALLDRFKVPLGRFDAAVYRRDVAQNSDFRKFDDGLKMTVDLDRHRSREIETL
                     LEQAARKGICQYGLHRQETALMTCIVPTPLQRDHMHFIDGGAGGYAMAASRLKAAAS"
     gene            585..1100
                     /locus_tag="AC16A0_08175"
     CDS             585..1100
                     /locus_tag="AC16A0_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024104.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3105350.1"
                     /translation="MQTELSMLDRFHFRLKSQHACFAWVFAQMADMQGLVFELGLGKG
                     RTYDHLRRNQPGREIYVFERDVRPIEGCLPPDPYLVRGDIEVNLPVYVKRFEGKVALV
                     HSDVGDISLEHNQMMRELVPRIAPPALMPGGFLLSDLNLEVPGFAQLPLPPDAVPGRY
                     FIYRKPLTAGS"
     gene            complement(1095..>2424)
                     /locus_tag="AC16A0_08180"
     CDS             complement(1095..>2424)
                     /locus_tag="AC16A0_08180"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR3105351.1"
                     /translation="TTVTLSSLSWPLLKRSGYTPTVAGGLTSAAGIGATLSPPTLGAA
                     AFIIAEYLEIDYVDVLIMAAIPTVLYYLSCWFMVEGDARKLGVTPVRTSDESLWGLTT
                     RKGYHFISLAAIAVLLATGMSAFLAVFWSIIIAFALSMIRAEDRLLAPYGAIAGLAVF
                     ALAWALGVRLSVSAYLGLMAGGGVTLLLHALGNDPDRAGPRLVDTFVEGGKSCVGIAA
                     TCATAGIIVSIINLSGLGLKLSGVIVDIGGGNLGMTLLLAAAAMWLLGTAVPVTASYI
                     IAAVVLVPALVSLGVAPQAAHMFMFYYAVLADVSPPTALSPFAAAAICGGAPFATMMQ
                     AWKYTLPAFVVPVIFCLPPFGTGLLLDGSPAEIALITITATAALAGLSLAAVGWMRGP
                     LSGIERLIAALAGVALIVPSPASQVAGAAALASVVGWQAYRFRQARQPGL"
BASE COUNT          466 a          803 c          794 g          361 t
ORIGIN      
        1 gggaggaaga gcgcggcgga caccccattc ccgaagtggg gccggagacc agtttcaagc
       61 cgggcaatac gacctatgaa acccgcgccg gaggcgcacc gctgttgcgg ccgctgcgcc
      121 gcctcggcat cattgcccag gtctggctgg ttgcgctcct cgaccggttc aaggtgccat
      181 tgggcaggtt cgatgctgcg gtctatcgcc gcgacgttgc gcagaactcc gactttcgca
      241 agttcgatga cggcctcaag atgacggtcg atctcgaccg gcatcgctcg cgcgaaatcg
      301 aaacgcttct cgaacaggcc gcccgaaagg gcatctgcca gtatggactg cacaggcagg
      361 aaaccgcgct gatgacctgc atcgttccga caccgcttca gcgcgaccac atgcatttta
      421 tcgacggcgg ggccggcggc tacgcaatgg ccgccagccg tctcaaggcg gccgccagct
      481 gaacgtcaca tgtcggccct gcagggggtt gcattgagcc cgcgcggttg tgctagacag
      541 cgcccagcaa gaagatcgat ggcgaaacgt caagggaccc cgcgatgcag accgaactga
      601 gcatgctgga tcggtttcac ttccggctga agtcgcagca tgcctgcttt gcctgggtct
      661 tcgcgcagat ggcggacatg cagggcctcg tcttcgaact gggcctcggc aagggccgca
      721 cctatgacca cctgcgacgc aatcagccag ggcgcgaaat ctatgtcttc gagcgtgacg
      781 tgcgccccat cgaaggttgc ctgccacccg atccctatct ggtgcgcggc gacatcgaag
      841 taaacctgcc ggtctatgta aagcgcttcg agggcaaggt cgcactggta cattccgacg
      901 tgggcgacat ttcgctcgaa cacaaccaga tgatgcgcga gcttgttccg cgcatcgcgc
      961 ctccggccct gatgccgggt ggctttctcc tgtctgatct caatctcgaa gtcccgggct
     1021 ttgcgcaact gcccctgccg cctgatgccg tgccgggccg ctacttcatc taccgcaagc
     1081 cgctgacggc cggctcatag acccggctgc cgggcctgcc ggaaccggta cgcctgccat
     1141 cccacgacgg aagccagagc cgcggcacct gccacctggc ttgccgggga gggaacgatc
     1201 agtgccacgc cggcaagggc cgcaatcagt cgctcgatgc ccgagagcgg accccgcatc
     1261 cagccgacgg ccgcaagcga caggccggcc agcgctgcgg tggctgtgat cgtgatgagc
     1321 gcgatctctg caggactgcc gtccagcagg agccccgtgc cgaagggcgg caggcagaag
     1381 atgaccggaa ccacgaaagc cgggagcgtg tacttccagg cctgcatcat ggtggcgaaa
     1441 ggcgcgcccc cgcagatggc ggcggccgcg aagggcgata gcgcggttgg cggcgagaca
     1501 tcggccagca ccgcatagta gaacatgaac atgtgggctg cctgcggcgc cacgcccagc
     1561 gataccaggg ccggcacaag caccacggcg gcaatgatgt aggaagcggt caccggcacc
     1621 gccgtgccga gcagccacat ggctgcggcc gcgaggagca gggtcatgcc gagattcccg
     1681 ccgccaatgt cgacgatgac gcccgacagc ttgaggccca ggccggagag gttgatgatc
     1741 gacacgatga tgccggcggt ggcgcaggtc gcggcaattc cgacgcaact cttgccgccc
     1801 tccacgaagg tgtccaccag ccgcggcccg gcgcggtcgg gatcattccc cagggcatgg
     1861 agcagcagcg tgacgccccc gcccgccatc aggccgagat aggccgaaac cgaaagccgc
     1921 acgcccagcg cccaggccag cgcgaagaca gccaggcccg cgatcgcgcc atagggtgcc
     1981 agcaggcggt cctcggccct gatcatgctg agcgcaaagg cgatgatgat cgaccagaac
     2041 accgcgagga aggcgctcat gccggtggcc agcagcaccg cgatcgcggc gagcgagatg
     2101 aagtgatagc ccttgcgggt cgtcagtccc cagagcgact catcgctcgt cctgaccggc
     2161 gtcaccccca gcttgcgcgc gtctccctcc accatgaacc agcagctgag atagtagagg
     2221 accgtcggga tggctgccat gatcagcacg tcgacgtagt cgatctccag atactcggcg
     2281 atgatgaagg cggcagcccc gagagtcggc ggcgagagcg tggcgccgat gccggctgca
     2341 gaggtcaggc cgcccgcaac cgtcggcgtg tagccggacc gcttcagcag cggccaggac
     2401 agtgacgaga gcgtcacggt cgtg
//
LOCUS       DBNGVR010000384         3107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3481914, whole genome shotgun sequence.
ACCESSION   DBNGVR010000384 DBNGVR010000000
VERSION     DBNGVR010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3481914"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..482
                     /gene="dut"
                     /locus_tag="AC16A0_08185"
     CDS             84..482
                     /gene="dut"
                     /locus_tag="AC16A0_08185"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR3105352.1"
                     /translation="MPLPAYETDGAAGMDLRAAEALVLKPGTRCLMPTGLAIALPQGF
                     EAQVRPRSGLAVKHGVTVLNAPGTIDCDYRGEIKVPLINHGDADFIIARGDRIAQMVI
                     APVTRATWEHVATLDGTARGEGGFGSSGRR"
     gene            482..1297
                     /locus_tag="AC16A0_08190"
     CDS             482..1297
                     /locus_tag="AC16A0_08190"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR3105353.1"
                     /translation="MALSGEEIERYARHLVLHEVGGPGQAKLKAARVLVIGAGGLGSP
                     LLLYLAAAGIGTIGVMDHDTVSLSNLQRQILHTTARVGMAKTASAREALLAINPHVTV
                     MAHDQRITAENALDIIGRYDIVADGCDNFPTRFLVSDACYFARRTLVSAAVGQFDGQL
                     STFKPHESSPDGTPWPSYRCLIGEIPPRGLFPACEEAGILGALPGIIGSMQAMEVIKE
                     VLGIGESLAGRLLMYDALAARFYETRLGWNPDNPLTGRRPSIAGIAAQNYAAA"
     gene            1416..2657
                     /locus_tag="AC16A0_08195"
     CDS             1416..2657
                     /locus_tag="AC16A0_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HIR3105354.1"
                     /translation="MAWTGTESRSDAGHALSRGTSQSGVRLYNERLVLSLVRHHGALP
                     KAEIARLTGLSAQTVSVIVRQLESDDLLRRETPQRGKVGQPLVPFTLNPEGAYAIGLK
                     LGRRSADLMLLDLTGRVCATLRQPYRYPVPAEIVSFTESGVKLLTKGMTRSQVARIAG
                     LGIAAPFELWNWSEAVGAPPGALDVWQGFDLGAAIAARVEWPVHHCNDATAACAAELL
                     FGNGRSRRDYAYFYVGFFIGGGIVINGGLHQGRSGNAGALGSLLVPSRSDGAEQLILS
                     ASLSVLEDALRRDGLDPDILRNCPDDWSAAGAVLDSWIADAAMSLACASASTAAVLEP
                     EAVIIDGAMPAAVRRRLVASVRSAMSKVATLGITPFVVEEGTIGADARAMGAASLALL
                     ANFIIDRDVLFKDVSAGASGS"
     gene            complement(2644..>3107)
                     /locus_tag="AC16A0_08200"
     CDS             complement(2644..>3107)
                     /locus_tag="AC16A0_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3105355.1"
                     /translation="CLGGGCELAMMCDFIIASETARFGLPEITLGIMPGAGGTQRLPR
                     AIGKAKAMDMIMTGRMMEAAEAERCGLVARIVAPERVLDEALAAAAKIASYSQPIVMM
                     AKETVNRAQEMSQEEGIRFERRLFLSMFATEDQKEGMAAFIEKRKPGFRNR"
BASE COUNT          500 a         1063 c          999 g          545 t
ORIGIN      
        1 cggctgatgg atcggcttgc cgccatgctg aaggaacgcg cgtgaccgcc gtccgtatcc
       61 gcaggctcgc ccatggctag gatctgccgc ttccggccta tgagacggat ggcgcggccg
      121 gcatggacct gcgcgccgcc gaagccctcg tgctgaagcc cggtacccgc tgcctcatgc
      181 cgacgggcct tgccattgcg ctgccgcagg gtttcgaggc gcaggtgcgt ccgcgatcgg
      241 gactcgcggt gaagcatggc gtgacggtgc tcaatgcgcc cggcactatc gactgcgact
      301 atcgcggcga gatcaaggtg ccgctgatca atcacggcga tgccgatttc atcatcgcgc
      361 gcggcgaccg tatcgcgcag atggtgatcg cgccggtgac gcgggcgacc tgggaacatg
      421 tcgctacgct cgatggcacg gcgcgcggcg aaggcggctt cggatcctcc gggagacgct
      481 gatggcgctg agcggcgagg agatcgaacg ttacgcccgg caccttgtgc tgcatgaggt
      541 cggcggtccg ggacaggcga agctcaaggc cgcgcgcgtg cttgtcatcg gcgcgggcgg
      601 tctcggctcg ccgctcttgc tctatctcgc cgccgccggc atcggcacca tcggcgtgat
      661 ggatcacgac acggtgagcc tgtccaatct acagcgccag atcctgcaca ccaccgcacg
      721 cgtgggcatg gccaagaccg ccagcgcgcg cgaggccctg ctggcgatca atccgcatgt
      781 gacggtgatg gcgcatgacc agcgcatcac cgcggagaac gccctcgaca tcatcggccg
      841 ctacgacatc gtggcggatg gctgcgacaa tttcccgacg cgctttctgg tgagcgacgc
      901 ctgctacttc gcccgcagga cgctggtgag cgcagcggtt ggccagttcg acgggcaact
      961 ctccaccttc aagccgcatg agtcgtctcc cgacggcacc ccctggccca gctatcgctg
     1021 cctgatcggc gagatcccgc cgcgcgggct ctttccggcc tgcgaggagg cgggaatttt
     1081 gggtgcgctc cccggcatca tcggatcgat gcaggccatg gaagtgatca aggaggtgct
     1141 gggcatcggc gaaagcctgg ccgggcgcct gctcatgtat gacgcgctgg cggcccgctt
     1201 ctatgagacc aggctcggct ggaatccgga caatccgctc accggccgcc ggcccagcat
     1261 tgcgggcatc gcagcgcaga attatgccgc cgcctgaatc cggcgcgggt ctttcaagct
     1321 ataaatcaga ccgatttact tattgactgc ccggacggcc tgtcggaagg tcgcgccacc
     1381 agccgccaga ggctggcatc ggcaggacgg gcagaatggc atggacaggc acggaaagcc
     1441 ggagtgacgc ggggcatgcc ctgtcgcgcg gcaccagcca gtccggcgta cggctttaca
     1501 acgaacgcct cgtactgtcg ctggtccgcc atcacggggc gctgcccaag gcggaaatcg
     1561 ccaggctgac cgggctttca gcccagaccg tttcggtgat cgtgcgccag ctcgaatccg
     1621 acgatctcct caggcgcgag acgccgcagc ggggcaaggt gggccagccc ctggtccctt
     1681 tcacgctcaa tcccgaaggt gcctatgcca tcggcctgaa acttgggcgc cggtcggccg
     1741 atctcatgct gctcgatctc accggccggg tctgtgccac tctccgccag ccctatcgct
     1801 atccggttcc ggccgagatc gtatccttca ccgaaagcgg cgtgaaactg ctgacaaagg
     1861 gcatgacccg cagccaggtc gcacgcatcg cggggctcgg catcgccgca cccttcgaat
     1921 tgtggaactg gtccgaagct gtcggcgcac cgcccggcgc tctcgacgtc tggcaaggct
     1981 tcgacctcgg tgcggcgatc gcggcgcgcg tggagtggcc cgttcatcac tgcaacgacg
     2041 caacggccgc ctgcgctgcg gaactcctct tcggcaacgg tcgctcccgg cgcgactatg
     2101 cctatttcta tgtcggcttc ttcatcggtg gcggcatcgt catcaatggc ggcctgcatc
     2161 agggccgcag tggcaatgcc ggcgcactcg gctcgctgct cgttccttcg cgatcggacg
     2221 gcgcagagca gctgattctc agcgcgtcac tgtcggttct cgaagatgcg ctgcggcgtg
     2281 acggccttga ccccgacatc ctgcgcaact gcccggatga ctggagcgct gccggcgcag
     2341 tcctcgacag ctggatcgcc gatgcggcca tgagccttgc ctgtgcctcg gccagcaccg
     2401 cagccgtgct cgagcccgag gccgtgatca tcgatggcgc catgccggcc gccgttcgcc
     2461 gcaggctcgt cgcttccgtg cgcagcgcca tgtcaaaggt ggcgacgctc ggaattacgc
     2521 ctttcgtcgt cgaagaagga accatcggtg ccgatgcgcg cgccatgggc gcagccagcc
     2581 tagccctgct tgccaacttc atcatcgacc gcgacgtgct gttcaaggac gtttccgccg
     2641 gagctagcgg ttcctgaagc caggcttgcg cttctcgatg aaggcggcca tgccctcctt
     2701 ctggtcttcg gtggcaaaca tcgacaggaa gagccgccgc tcgaagcgga tgccttcctc
     2761 ctgcgacatc tcctgggcgc ggttcacggt ctccttcgcc atcatcacga tgggctgcga
     2821 ataagatgcg atcttcgcgg ccgcagccag cgcttcatcg agcacccgct ccggcgccac
     2881 gatccgcgca accagcccgc agcgctcggc ctcggccgcc tccatcatgc ggccggtcat
     2941 gatcatgtcc atcgccttgg ccttgccgat ggcccgcggc aggcgctgcg tgccgccggc
     3001 acccggcatg atgcccaggg tgatctcggg aagcccgaac cttgccgtct cgctggcgat
     3061 gatgaagtcg cacatcatgg cgagttcgca gccaccgccg aggcaaa
//
LOCUS       DBNGVR010000385         4456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1713845, whole genome shotgun sequence.
ACCESSION   DBNGVR010000385 DBNGVR010000000
VERSION     DBNGVR010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1713845"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="AC16A0_08205"
     CDS             complement(<1..1307)
                     /locus_tag="AC16A0_08205"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3105356.1"
                     /translation="MALSDAILTRLLTLHPKIIDLSLGRMERLLAILGHPEHQLPPVI
                     HVAGTNGKGSTVSYLRHMMEAAGLVVHAYTSPHLVRFHERIRVAGALISEADLTALLE
                     ECEVANGGEPITFFEITTAAAFLAFSRIPADYLLLEVGLGGRLDATNVIDNPAVAVIT
                     TIDYDHQQYLGDTLALIAAEKAGILKRGRPAVIGVQPDEAREVLVRAAERLRCPMAVA
                     NQDWQAFEQHGRLVFQDESGLLDLPLPQLRGRHQIDNAGNAIAALRLLGDGRITDSHI
                     AEGLKTATWPARMQRLGSGALWSLVPADAELWLDGGHNPSAGRVLAQAFSDLNDRHSR
                     PLVLVWGMLNTKDVGRFIESFAGIAARVVTLTIPDEENAVSAEALAEAARAAGLSAET
                     ASSLDEALRQAALAAPAPRILICGSLYLAGRVLAAHASEEMSRV"
     gene            complement(1318..2214)
                     /gene="accD"
                     /locus_tag="AC16A0_08210"
     CDS             complement(1318..2214)
                     /gene="accD"
                     /locus_tag="AC16A0_08210"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3105357.1"
                     /translation="MNWIKNFVRPKIRSILGDKREVPENMWVKDPDSGQMVFYKDLEA
                     NQFVMPPSNYHMRMPPEDRLKATFDNGAYDRIAMPQVPADPLKFRDERKYADRIKEAR
                     SKTNADDCIQAGHGTLDDVPVTVAVQDFAFMGGSLGLAAGEAIITAMRAAVARKTPFI
                     FFVSSGGARMQEGVLSLMQLPRTTVAVQDLRAASLPYIVVLTHPTTGGVTASYAMLGD
                     VHIAEPGALIGFAGQRVIEQTIREKLPEGFQRAEYLQEHGMVDMVVHRHEMRDTLSRI
                     VRILMKVPDRSLPETALVPLAD"
     gene            complement(2219..3034)
                     /gene="trpA"
                     /locus_tag="AC16A0_08215"
     CDS             complement(2219..3034)
                     /gene="trpA"
                     /locus_tag="AC16A0_08215"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR3105358.1"
                     /translation="MSRIERRFASLKAEGRAALVTFVTAGDPDYETSLKIIKGLPGAG
                     ADVIEFGMPFTDPMADGPAIQAAGLRALAAGQTMKKTLKMVKAFRRDDQDTPVVLMGY
                     YNPIYVYGVDKFLADAKAAGADGFIIVDLPPEEDEEFCLPALKAGMNFIRLATPTTDD
                     ARAPVVFRNTSGFVYYVSVLGITGTKAPDLKSVKANVNRLRKHTDLPICVGFGVKTAE
                     QARAIAKDADGVVVGSALVGAIAASLTKAGKPTSKTAKAVHELVASIARGVRK"
     gene            complement(3031..4251)
                     /gene="trpB"
                     /locus_tag="AC16A0_08220"
     CDS             complement(3031..4251)
                     /gene="trpB"
                     /locus_tag="AC16A0_08220"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3105359.1"
                     /translation="MNLNAPNSFRTGPDENGLFGIYGGRFVAETLMPLILQLEQAYKD
                     AKADPSFQEELDSFLEHYVGRPSPLYFAERLTEHLGGAKIYFKRDELNHTGAHKINNV
                     MGQILLARRMGKKRIIAETGAGQHGVATATACARFGLECIVYMGEVDMERQKPNVFRM
                     KLLGAEVVPAKSGSRTLKDALNEALRDWVANVEDTFYCIGTVAGPHPYPEMVRDFQSV
                     IGRETKQQMLKREGRLPDSLVAAIGGGSNAMGLFHPFLDDKEVEIYGVEAAGLGLRTK
                     TKHAASITGGRPGVLHGNRTYLLMDDDGQIIEGHSISAGLDYPGIGPEHSWLHDMKRV
                     EYVSATDKEALAAFQLCCRLEGIIPALEPSHALAHVMKIAPKKPKDHLMVMNLCGRGD
                     KDIFTVADVLGVKL"
     gene            complement(4320..>4456)
                     /gene="trpF"
                     /locus_tag="AC16A0_08225"
     CDS             complement(4320..>4456)
                     /gene="trpF"
                     /locus_tag="AC16A0_08225"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3105360.1"
                     /translation="DTVAEAIRITGAAMVDVSSGVESAPGIKDVGLIRKFIEAAKRAG
                     "
BASE COUNT          739 a         1398 c         1510 g          809 t
ORIGIN      
        1 acgcggctca tctcctcgct ggcatgggcc gccagcacgc gtcccgcaag gtaaagcgag
       61 ccgcagatga gaatgcgggg cgcaggtgcc gccagcgccg cctggcgcag ggcctcgtcg
      121 agcgagctcg cggtctcggc cgaaagcccc gcggcccggg ccgcttcggc cagcgcttcg
      181 gcggagacgg cattctcctc gtccggaatg gtgagggtca ccacccgggc cgcgatgccg
      241 gcaaagctct cgatgaagcg gcccacgtcc ttggtgttga gcatgcccca gaccagcacc
      301 agtgggcggg agtggcggtc attcaggtcg gaaaaggcct gggccagcac ccgcccggcc
      361 gagggattgt ggccgccatc cagccacagt tccgcatccg ccggcacgag ggaccataat
      421 gcacccgatc ccagccgttg catgcgggca ggccatgtag cggtcttcag gccttcggcg
      481 atatgggaat ccgtgatgcg gccatcgccg agcagccgca gcgcggcaat ggcattgccg
      541 gcattgtcga tctgatgacg gccgcgcagc tgcggcaacg gcaggtcgag caggccgctc
      601 tcatcctgga agacgagccg gccatgctgc tcgaaggcct gccagtcctg gttggcgacg
      661 gccatcgggc agcgcaggcg ttcggccgcc cgcaccagaa cctcgcgcgc ttcgtcgggc
      721 tggacgccga tcacggccgg ccggccacgc ttcaggatgc cggccttttc tgccgcaatc
      781 agggccagcg tatcgcccag atactgctgg tggtcgtagt cgatggtggt gatcaccgcg
      841 actgccggat tgtcgatgac attggttgcg tccagccgtc cgcccagtcc cacttccagc
      901 agcagataat ctgccgggat ccggctgaag gcgaggaagg ctgcagcggt ggtgatctcg
      961 aagaaggtga tgggctcacc gccattggcg acttcgcatt cttccagaag ggctgtgaga
     1021 tcggcctcgg aaatcagtgc gcccgccacc cggatgcgct catggaacct caccagatgc
     1081 ggcgaggtat aggcatgcac caccaggcct gccgcttcca tcatgtggcg gagataggaa
     1141 accgtcgaac ccttgccatt ggtgccagcc acatggatga ccggcggcag ttggtgctcg
     1201 ggatggccaa ggatcgccag aagccgttcc atgcgcccca ggctgaggtc gatgatcttg
     1261 ggatgcagcg tgagcagccg cgtcaggatg gcatcgctga gcgccatggc gacggcgtca
     1321 gtccgcgagg ggaacgaggg ccgtctccgg caggctgcgg tctggcacct tcatcaggat
     1381 gcggacgatg cgcgagagcg tgtcgcgcat ttcgtgacgg tgtacgacca tgtccaccat
     1441 gccgtgctcc tgcaggtatt cggcgcgctg gaaaccctcg ggaagcttct cgcggatggt
     1501 ctgttcgatg acacgctggc cggcaaaacc gatgagcgcg ccgggttcgg cgatatgaac
     1561 gtcgcccagc atggcatagg aggccgtgac gccgccggtc gtcggatgcg tgaggaccac
     1621 gatatagggc aggctggcgg cccgaagatc ctgaaccgcc acggttgtgc gcggcagctg
     1681 catgagcgag aggaccccct cctgcatgcg ggcgccgccc gaggaaacga agaagatgaa
     1741 gggcgttttg cgtgccaccg ctgcgcgcat ggcggtgatg atggcttcac cggccgcaag
     1801 acccagcgag ccacccatga aggcgaagtc ctgcacggcc acggtgaccg gcacgtcgtc
     1861 gagcgtgcca tggccggcct ggatgcagtc gtcggcgttg gtcttgctgc gggcttcctt
     1921 gatgcggtcg gcatatttcc gctcgtcgcg gaacttcagc ggatcggccg gcacctgggg
     1981 catggcgatg cggtcgtagg cgccattgtc gaaggtggcc ttgagccggt cctccggcgg
     2041 catgcgcatg tgatagttcg acggcggcat gacgaactgg ttcgcctcga ggtccttgta
     2101 gaagaccatc tggccggagt cgggatcctt cacccacatg ttctccggca cctcgcgctt
     2161 gtcgccgagg atggagcgga ttttcgggcg gacgaagttc ttgatccagt tcatgcggtc
     2221 atttcctcac gccgcgcgcg atgctggcca ccagctcatg cacggccttc gccgtctttg
     2281 aggtgggctt tccggctttt gtgaggctcg cggcaatggc gccgaccagc gccgagccga
     2341 ccaccacgcc atccgcatcc ttggcgatgg cgcgcgcctg ctcggcggtc ttgacgccga
     2401 acccaacgca gatcggcaag tccgtgtgct tgcgcagccg gttgacattg gccttgacgc
     2461 tcttgaggtc gggcgccttg gtgccggtaa tgcccagcac cgagacgtag tagacgaagc
     2521 cgctggtgtt gcggaagacg actggcgcac gcgcatcgtc ggtggtgggc gtcgccagcc
     2581 ggatgaagtt catgccggcc ttgagcgccg gcaggcagaa ctcctcgtct tcctccggcg
     2641 gcaggtcgac gatgatgaag ccatcggcgc cggcggcctt tgcatcggca agaaacttgt
     2701 cgacaccata gacatagatc ggattgtagt agcccatgag gacgactggc gtatcctggt
     2761 cgtccctgcg gaacgccttc accatcttga gcgtcttctt catggtctgg cccgccgcca
     2821 gcgcgcgaag ccccgcggcc tggatcgccg ggccatcggc catggggtcg gtgaagggca
     2881 tgccgaactc gatcacgtcg gcaccggccc cgggcaggcc cttgatgatc ttcagcgagg
     2941 tctcgtaatc gggatcgccc gcggtcacga aggtgacaag ggcggcgcgg ccctccgcct
     3001 tgagggacgc gaagcggcgt tcgatgcggc tcacagcttc accccgagca catcggccac
     3061 ggtgaagatg tccttgtcgc cgcggccgca gagattcatc accatgagat gatccttcgg
     3121 cttcttcggg gcgatcttca tgacatgggc cagcgcatgc gagggctcca gcgccgggat
     3181 gatgccctcg agcctgcagc agagctggaa ggcggcaagc gcctccttgt cggtggccga
     3241 aacatattcc acgcgcttca tgtcatgcag ccaggagtgt tccgggccga tgccggggta
     3301 gtcgaggccg gccgagatgg agtggccctc gatgatctgg ccgtcgtcgt ccatcagcag
     3361 ataggtgcgg ttgccatgca gcacaccggg ccggccgccg gtgatggagg cggcatgctt
     3421 cgtcttggtc ctgaggccga ggcccgcggc ttcgacgccg tagatttcca cctccttgtc
     3481 gtcgaggaag ggatggaaca ggcccatggc gttcgagccg ccgccgatgg cggcgaccag
     3541 cgagtccggc aggcgtcctt cgcgcttcag catctgctgc ttggtctcgc ggccgatgac
     3601 cgactggaag tcgcgcacca tttccggata gggatgcggg cctgccaccg tgccaatgca
     3661 gtagaaggta tcctcgacat tggccaccca gtcgcgcagc gcctcgttga gcgcgtcctt
     3721 gagcgtgcgc gagcccgact tggccggcac cacctcggcg cccagcagct tcatgcggaa
     3781 gacgttgggc ttctgccgct ccatgtcgac ttcgcccatg tagacgatgc attcgaggcc
     3841 gaagcgggcg caggcggtgg cggtggccac gccatgctgg ccggcgccgg tctcggcgat
     3901 gatgcgcttc ttgcccatgc ggcgggcgag caggatctgg cccatcacgt tgttgatctt
     3961 gtgcgcgccg gtatggttca gctcgtcgcg cttgaaatag atcttggccc cgccgagatg
     4021 ctcggtgagg cgttcggcga agtagagcgg cgagggccgg cccacataat gttcgaggaa
     4081 actgtcgagc tcttcctgga agctcgggtc ggccttggca tccttgtagg cctgctcgag
     4141 ctgcaggatc agcggcatca gcgtctcggc cacgaagcgg ccgccataaa tgccgaagag
     4201 gccgttttcg tctgggccgg tacggaagga attgggtgcg ttgaggttca tcgtggtgcc
     4261 tgaataccct gttgcgctga cgggttaatg cccgccgcct tgcaatacgt gggagggttc
     4321 tacccggccc gcttcgccgc ctcaatgaat ttccggatca ggcctacatc cttgatgccg
     4381 ggggcggatt cgacgccgga ggagacatcg accatcgccg cgccggtgat cctgatagcc
     4441 tcggcaaccg tgtccg
//
LOCUS       DBNGVR010000386         4973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1519940, whole genome shotgun sequence.
ACCESSION   DBNGVR010000386 DBNGVR010000000
VERSION     DBNGVR010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1519940"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /gene="secA"
                     /locus_tag="AC16A0_08230"
     CDS             <1..1749
                     /gene="secA"
                     /locus_tag="AC16A0_08230"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3105361.1"
                     /translation="DEFTGRMMPGRRYSEGLHQALEAKEHVAIQPENVTLASITFQNY
                     FRLYDKLAGMTGTASTEAEEFMDIYGLDVIEIPTNVPVARMDEDDQVYRTAREKYAAI
                     IDVINEARERGQPVLVGTTSIERSEVLSSMLKSKGVPHNVLNARYHEQEAAIISQAGV
                     PGGVTIATNMAGRGTDIKLGGNLEMRIATELANLPEGAERQSRIARIKDEIEAARQKV
                     LAAGGLFVIGTERHESRRIDNQLRGRSGRQGDPGRSLFYLSLEDDLMRIFGSERMDGM
                     LQKLGLKDGEAIVHPWINKALEKAQQKVEARNFDIRKNLLKFDNVMNDQRKVIFEQRI
                     GIMKGEEVSETIDDMRHQVINDMVTKHIPPNAYAEQWDAAGLRERLREVIAIDYPVDE
                     WAKEEGIADEEILERVTKAADDLYAGKREKYGPEIMGQVEKAVLLRTLDNLWRDHIIT
                     VDQLRQVIHLRGYGQRDPLNEYKTEGFTLFENMISKLRENTTAQVMRVEVQTRPADDM
                     LPDEDELPMMEAHHIDALTGEDELAEGPLLTVTPGKTAGKMDPKKPDTWGKVSRNDPC
                     PCGSGKKYKHCHGAYV"
     gene            complement(1762..3195)
                     /locus_tag="AC16A0_08235"
     CDS             complement(1762..3195)
                     /locus_tag="AC16A0_08235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3105362.1"
                     /translation="MLTRRAILAGLGAMPAMLGSVGRARAGTRRNLSIESRTLDVQGR
                     DAKVYGLSVNGEFKGLDLLLGEQFNVRLTNNLNVESLIHWHGLTPPVQFDGVPMLSQA
                     PLRPGAAFDYDFPVLQSGTHWMHSHVGLQEQQLLAAPLIVRETGTPVFDEQEHVVMLH
                     DFTFRDPAEILAELQGGGGAHAAHAGHGVDHSKMDHAAQPAAPAMSGAMLNDVVYDAY
                     LANDRTLDDPETLQVEKGGWLKLRIINGAGASNMWVDLGALTGSLVAVDGHAIHPIPG
                     SRFPLAIAQRADIRLQIPREGGAFPILFRPEGVSARTGIVLTTPDAAVQRIAATADVA
                     PALDLSFERSFRAVAQLPDEPVTRTEMLMLTGGDAGYNWGLNGKSSMHDVLFTVRMGE
                     RVEVMMHNMTSMSHPMHLHGHYFKVVAIGSERIDGAVRDTVLVPPGETVTIAFDANNP
                     GNWAFHCHHVYHMNAGMMGAIAYRDAA"
     gene            3506..3688
                     /locus_tag="AC16A0_08240"
     CDS             3506..3688
                     /locus_tag="AC16A0_08240"
                     /inference="COORDINATES: protein motif:HMM:NF016828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR3105363.1"
                     /translation="MTHIAGKFGKFLRDSKGATAIEYALIASIMGVMIVPVLSDTTSG
                     VASLYTKIQGLFDSLN"
     gene            complement(3695..4648)
                     /locus_tag="AC16A0_08245"
     CDS             complement(3695..4648)
                     /locus_tag="AC16A0_08245"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR3105364.1"
                     /translation="MPSFLDFEAPVAELEGKIAELRALAAGDKTVSIAEEVKSLEKKA
                     AKSLSDLYQNLDPWQKAQVARHPERPHATDYIGSLITEFTPMAGDRKFAEDFAVIGGL
                     GRFRGEPVMVIGQEKGNDTASRLKRNFGMARPEGYRKAVRLMELAQRFGLPVITFVDT
                     AGAYPGIDAEERGQAEAIARATDCCLGLGVPLVSVIIGEGGSGGAIAIATANTVLMLE
                     HAIYSVISPEGAASILWRDAARAKDAAAAMKITAQDLKKLGIIDEIIGEPLGGAHRGR
                     ADVIAATGDALSRTLKAYRNVSGDVLRTQRREKFLAIGRSL"
     gene            complement(4703..>4973)
                     /locus_tag="AC16A0_08250"
     CDS             complement(4703..>4973)
                     /locus_tag="AC16A0_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3105365.1"
                     /translation="GQSGHLTRQHFALSLKDLAREAGIDAAKISPHVLRHGFASHLLA
                     GGADLRAVQQMLGHADISTTQIYTHVQSERLRQVVETHHPLAKKS"
BASE COUNT         1022 a         1559 c         1527 g          865 t
ORIGIN      
        1 gacgagttca ccggccgcat gatgcccgga cggcgctatt cggagggcct gcatcaggcc
       61 ctcgaggcca aggagcacgt ggccatccaa cccgagaacg tcacgctggc ctccattacc
      121 ttccagaact acttccgcct ctacgacaaa ctcgccggca tgaccggcac cgcctccacc
      181 gaagccgaag agttcatgga catttatggg ctcgacgtga tcgagatccc caccaacgtg
      241 cctgtggcac gcatggacga ggatgaccag gtctatcgta ccgcccgcga gaaatatgcc
      301 gccatcatcg acgtcatcaa cgaggcccgc gaacgcggtc agcccgtgct cgtgggcacc
      361 acctcgatcg agagatcgga agtgctttcc tccatgctga agtccaaggg ggttccgcac
      421 aacgtgctca acgcccgcta tcacgagcag gaggcggcga tcatctccca ggccggcgtg
      481 ccggggggcg tgacgatcgc caccaacatg gccggccgcg gaaccgatat caagctgggc
      541 ggcaatctcg agatgcgcat cgccaccgaa cttgccaacc tgcccgaggg tgccgagcgc
      601 cagtcacgca ttgcccgcat caaggacgag atcgaggccg ccaggcagaa ggttctggcc
      661 gcaggcggtc ttttcgtcat cggcaccgaa cgccacgaga gccgccgcat cgacaatcag
      721 ctgcgtggcc gctcaggacg ccagggcgac cctggccgct cgctattcta tctttcgctg
      781 gaagacgacc tgatgcgcat cttcggctcg gagcgcatgg acggcatgct gcagaaactg
      841 ggactgaagg acggcgaagc catcgtccac ccctggatca acaaggcgct agagaaggcg
      901 cagcagaagg tcgaggcgcg caacttcgac atccgcaaga acctgctcaa gttcgacaac
      961 gtgatgaacg atcaacgcaa ggtgatcttc gaacagcgca tcggaatcat gaagggcgaa
     1021 gaggtttccg agaccatcga cgacatgcgt caccaggtga tcaatgacat ggtgaccaag
     1081 cacatccccc cgaacgccta cgccgagcag tgggacgccg caggcttgcg cgagcggctg
     1141 cgcgaagtga tcgccatcga ctatccggtg gacgagtggg ccaaggaaga gggaatcgcc
     1201 gacgaggaga tcctcgagcg cgtcaccaag gcggccgacg acctctacgc cggaaagcgc
     1261 gagaaatacg gtcccgagat catggggcag gtcgagaagg ccgtgctgct gcggacactc
     1321 gacaatcttt ggcgcgatca catcatcact gtcgaccagc tgcgccaggt gatccatctc
     1381 cgcggctatg gccagcgcga tccgctgaac gagtacaaga ccgagggctt cacgctcttc
     1441 gagaacatga tcagcaagct gcgcgagaac acgaccgcgc aggtcatgcg ggtcgaagtc
     1501 cagaccaggc cggccgacga catgcttcct gacgaagacg agctgccaat gatggaggcc
     1561 caccacatcg acgctctcac cggagaagac gaactggcag aaggcccgct cctgacggtt
     1621 acgcctggca agacggcagg caagatggac cccaagaagc cggatacctg gggcaaggtc
     1681 agccgaaacg acccctgccc ctgtgggtca ggcaagaaat acaagcactg ccacggcgcc
     1741 tatgtctgaa ccagcgcggt tctaggccgc gtcgcgatag gcaattgccc ccatcatgcc
     1801 ggcgttcatg tgatagacat gatggcagtg gaaggcccaa tttcccggat tgttggcatc
     1861 gaaggcaatg gtgacggttt cgcccggcgg aaccaggacc gtgtcgcgca cggcgccgtc
     1921 gatgcgctcg gaaccgatgg caaccacctt gaagtagtgg ccatgcagat gcatgggatg
     1981 agacatcgaa gtcatgttgt gcatcatgac ctccacccgc tcgcccatgc gcacggtgaa
     2041 cagcacgtca tgcatcgatg actttccgtt gaggccccag ttgtacccgg cgtcgccgcc
     2101 ggtcagcatc agcatctcgg tgcgcgtgac aggttcgtcc ggaagctggg caacggccct
     2161 gaagctgcgc tcgaaactca ggtccaatgc gggcgcaaca tcggccgttg cggcaatccg
     2221 ctggaccgcg gcatcgggcg tggtcagcac gatgccggta cgcgcgctga cgccttcggg
     2281 ccggaacagg atggggaacg cgccaccttc cctgggtatc tgcagccgga tgtcggcgcg
     2341 ctgagcgatt gcaagcggga agcgcgaccc gggaatggga tgaatggcat ggccgtcgac
     2401 ggccaccagg ctgcccgtca gcgcgccgag atcgacccac atgttggatg cgccggcacc
     2461 attgatgatg cgcagcttga gccaaccacc cttttcgacc tggagagttt ccggatcgtc
     2521 gagggtgcga tcattggcga gataggcatc atagaccacg tcgttcagca tggcgccgga
     2581 catcgccggt gccgccggct gggcggcatg atccattttc gagtgatcca ccccatggcc
     2641 ggcatgggcc gcatgcgcgc cgccgccgcc ctgcagttcg gcaaggatct ctgccgggtc
     2701 gcggaacgtg aagtcatgca gcatgacgac gtgctcctgc tcgtcgaaga ccggcgtgcc
     2761 ggtctcgcgc acgatcagcg gcgcggccag cagctgttgt tcctgcaagc cgacatggga
     2821 atgcatccag tgcgttccgg attgcaggac cggaaagtca taatcgaagg ctgcacccgg
     2881 ccggagtggc gcctgcgaca gcatcggaac cccatcgaac tgaaccggcg gcgtcaggcc
     2941 atgccagtga atcagggatt cgacattgag attgttggtt aaccggacgt tgaactgttc
     3001 gcccagcaac agatccagac ctttgaactc accgttgacg ctcagtccat agacctttgc
     3061 atcgcggccc tgcacgtcaa gcgtgcgcga ctcgatggaa agattgcgcc gggttccggc
     3121 ccttgcccgg ccaaccgacc ccagcatcgc cggcattgca cccaatccgg ccaagatggc
     3181 ccgccgcgtg agcatcctgt tctccatatg attgatctgc ttcgagacat aagtggcatt
     3241 ccgccctgtc acaaggcggc cgctggtcac agtttcgcgt ccctgcgtga tcacaaaggc
     3301 agaacgcctc aatttaaggt taaccttggg cggcctgaga aacctgaaaa aacgccattt
     3361 tcagctgatg taggtaccta cataaacgca aacagaaaca ccgagtgaca atcttaagtt
     3421 ccgcattaag gactccttga aacgatccat ggtttaacat gcgtatctga agccggtatc
     3481 accggatgcg atggagcccc gagacgtgac acacattgca ggcaagtttg gcaaattcct
     3541 ccgggattcg aagggcgcga ccgcaatcga gtatgccctg atcgcatcga tcatgggtgt
     3601 gatgatcgtt ccggtcctga gcgacacgac gtcgggcgtc gcctctctct atacaaagat
     3661 ccagggtctc ttcgacagtc tcaactgatc tggttcagag gcttctgccg atcgccagga
     3721 acttctcgcg gcgctgcgta cgcagcacgt ctcccgaaac attacgatag gccttgagcg
     3781 tccggctgag cgcgtcgccc gttgcggcga tcacgtctgc ccggccgcga tgcgccccgc
     3841 ccagcggctc gccgataatc tcgtcgatga tgcctagctt cttgaggtcc tgcgccgtga
     3901 tcttcatggc agcggcggca tccttggcgc gggctgcgtc gcgccacagg atggaggccg
     3961 caccctcggg cgagatcacg ctgtagatcg catgctccag catcagtacc gtattggccg
     4021 tcgcgatggc gatggcaccg cctgacccac cttcgccgat gatcacggaa accagtggaa
     4081 caccgaggcc aaggcagcag tccgtggccc gcgcgatggc ttcggcctgc ccgcgttcct
     4141 cggcatcaat gccgggatag gcgccggcgg tatccacgaa ggtgatcacc ggcaagccga
     4201 accgctgggc cagctccatc aggcggactg ccttgcggta gccttcgggc cgcgccatgc
     4261 cgaagttgcg cttgaggcga gaggcggtat cgttgccctt ctcctgaccg atgaccatga
     4321 caggctcgcc ccggaaacgg ccaagcccgc cgatcaccgc aaagtcctcg gcgaacttgc
     4381 ggtcgcccgc catgggcgtg aactcggtga tgagggaccc gatatagtca gtggcatgcg
     4441 gccgctcggg atggcgggcc acctgggcct tttgccaggg atcgaggttc tgatagagat
     4501 cggacaggct cttggcagcc ttcttctcga gcgacttcac ctcttccgcg atggagacgg
     4561 tcttgtcgcc ggcggcgagc gcgcgcagtt cggcgatctt gccttcgagt tcggcgacgg
     4621 gcgcttcaaa atcgagaaaa cttggcatga cagtggatcg atccttggaa aggccgataa
     4681 ctggccgtcc gcgtccggga agtcaacttt tcttggcaag agggtgatgg gtctcgacca
     4741 cctgccgcaa ccgctcggac tgcacatggg tgtagatttg tgtcgtcgat atgtccgcat
     4801 ggcccagcat ctgctggacc gcccgcagat ccgccccccc ggccaggagg tggctggcga
     4861 agccgtggcg cagcacgtgc ggagagatct ttgcggcatc gatcccggcc tcgcgggcca
     4921 gatccttcag cgacagggcg aagtgctgcc gcgtcaggtg gcctgactgg ccg
//
LOCUS       DBNGVR010000387         4019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4072210, whole genome shotgun sequence.
ACCESSION   DBNGVR010000387 DBNGVR010000000
VERSION     DBNGVR010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4072210"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..651
                     /locus_tag="AC16A0_08255"
     CDS             124..651
                     /locus_tag="AC16A0_08255"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR3105366.1"
                     /translation="MTLYALDGISPDLPGEGLCWVAPDAAVIGKVKLERMASVWFGAV
                     LRGDNELLLVGENSNIQDGCVLHSDMGSPLTIGRNCTIGHKAILHGCSIGDNSLIGMG
                     ATVLNNAQIGRNCLIGANALVTEGKVIPDNSLVMGSPGKVVRELDAAAAAKLPLSAEG
                     YVRNWQRFARGLRAL"
     gene            complement(638..1390)
                     /locus_tag="AC16A0_08260"
     CDS             complement(638..1390)
                     /locus_tag="AC16A0_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3105367.1"
                     /translation="MVHRKPRTPISRSRKRASSLHLQLAGAIGEGILNATYAPGTLLP
                     NEAEWGAMFGASRTAVREAIKTLNGKGLLVSRPKIGSRVEPRERWNFLDRDVLAWHCA
                     AMDRKQLLLSIQEMRRIFEPGAAALAAERRTEAQVARIAAALEGMGAARNAEEMVAPD
                     VEFHLALLEAANNELLIPFGIIIEQALRQMFDYTSRHNPRPLGVIPLHAAVFEAVRKQ
                     RPDAARKAVVTLLRDTDNVITAVVPARRPRVP"
     gene            1587..2609
                     /gene="chvE"
                     /locus_tag="AC16A0_08265"
     CDS             1587..2609
                     /gene="chvE"
                     /locus_tag="AC16A0_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012758577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3105368.1"
                     /translation="MAAGMMPALAEGKGLVGISMPTKSSTRWISDGESMVKEFTALGY
                     DTDLQYAEDDIPNQLAQIENMVTKGAKVLIVAAIDGTTLSDILKKANESGAKVIAYDR
                     LIRDSGNVDYYSTFDNFKVGVLQAESLVAGLKERFPGVKPWNVELFGGSPDDNNAFFF
                     YDGAMSVLQPMIDAGEIVVPSGQMGMDKVGTLRWDGAVAQSRMDNLLSANYTDKKVHA
                     VLSPYDGLSRGIISSLKGVGYAPGPEMPIVTGQDAEVASVKAMQAGEQYSTVFKDTRE
                     LAKTTAGMVDAVMQGKEPAINDTKTYNNGVKVVPSNLLVPYSVGKDDIQKMLVDSGYI
                     KAEELQ"
     gene            2799..3251
                     /locus_tag="AC16A0_08270"
                     /pseudo
     CDS             2799..3251
                     /locus_tag="AC16A0_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            3468..>4019
                     /locus_tag="AC16A0_08275"
     CDS             3468..>4019
                     /locus_tag="AC16A0_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3105369.1"
                     /translation="MVGRERTDRYPPRQAKVQDTIFEVKNWNVFHPLHADRQIIKDVN
                     LHVKKGEVVGIAGLMGAGRTELAMSIFGRAYGQRISGEVLLHGSPVDVSTIPKAMDAG
                     LAYATEDRKTYGLVLIDHIKHNVTLANLDGVSSRGVIDDMHELTVANKYRNDLRIRSA
                     SVFQATVNLSGGNQQKVVLSKWLF"
BASE COUNT          813 a         1213 c         1244 g          749 t
ORIGIN      
        1 ttggctcgat tgactttcat ccggtctccg caatcgttct aacgttcatc ggagtggtct
       61 ggagcttctt gcagggtgtg tttatcctga cgaccttctt tggcttcacc tgacggaggt
      121 gacatgaccc tctatgcgct cgacggcatc tcccccgacc tgccggggga gggcctttgc
      181 tgggtggcgc cggatgccgc cgtgattggc aaggtgaagc tcgaacgcat ggccagcgtc
      241 tggttcggcg cggtactgcg cggcgacaac gagcttctgc tggtcggcga gaacagcaac
      301 atccaggacg gctgcgtcct gcattccgac atggggtctc cactgaccat tgggcgcaac
      361 tgcaccatcg gccacaaggc aatcctccac ggctgcagca ttggcgacaa ctcgctcatc
      421 ggcatggggg caacggtgtt gaacaatgcg cagatcggac gaaactgcct gatcggcgcc
      481 aatgccctgg tcactgaagg caaggtcatc cccgacaatt ccctggtgat ggggtcgccc
      541 ggcaaggtgg tgcgggaact cgacgcggcg gcggccgcaa agctgccact ttcggcagaa
      601 ggctatgtac ggaactggca gcgcttcgcc agggggctca gggcactcta ggccgccgcg
      661 ccgggacgac cgccgtgatt acattgtccg tgtcgcgcag cagggtgacg acggctttcc
      721 gggccgcatc cgggcgttgc ttgcgtaccg cctcgaaaac cgcggcatgc agcggaatga
      781 cacccagcgg ccgggggttg tgccgggatg tgtagtcgaa catctggcgc agcgcctgtt
      841 cgatgatgat gccgaaggga ataagcagct cattgtttgc cgcttccagc agggcaaggt
      901 ggaattcgac gtcgggtgcc accatttcct cggcattgcg cgcggcgccc atgccctcga
      961 gcgccgcggc aatgcgtgca acctgcgcct cggtacgccg ctccgcagcc aaggccgcgg
     1021 cgccgggctc gaagatgcgg cgcatttcct ggatcgacaa gagcaactgc ttgcggtcca
     1081 tagcggcgca atgccaggcc aggacgtcac gatcgaggaa gttccagcgt tcgcgcggtt
     1141 cgacgcggct gccgatcttg gggcgcgaca cgagcaggcc cttgccattg agcgtcttga
     1201 tggcctcgcg cacagccgtg cggctggcac cgaacatggc accccattcg gcctcgttgg
     1261 gaagaagcgt gccgggcgcg taggttgcgt tcagaatacc ctcgccgata gctccggcca
     1321 gttgcagatg aaggctgctt gcacgcttgc ggctgcgcga gatcggtgtt cttggtttgc
     1381 gatgcaccat cgcggaccct ccctgggacc gccatttatc gtacaaataa tcgttgatta
     1441 ccagtcctcg atatcctaat gaaccagaca tgacaacggg gctgacaacc agagaacgcc
     1501 cgctttgtca tgcgttcaca aagggaggaa cacacgtgat caatcgcaga tatttcatcg
     1561 catccgccgc cggcatcgca accgtgatgg ccgccggcat gatgccggct ctggcggaag
     1621 ggaagggcct cgtgggtatt tcgatgccga ccaagtcctc gacccgctgg atctccgacg
     1681 gcgagtccat ggtgaaggaa ttcaccgccc tcggctatga caccgatctt cagtatgccg
     1741 aagacgacat tccgaaccag cttgcccaga tcgagaacat ggtgaccaag ggtgccaagg
     1801 tcctgatcgt cgcggccatc gacggcacca cgttgtctga catcctgaag aaggcgaatg
     1861 agtcgggcgc caaggtcatc gcctacgacc gcctgatccg cgattccggc aatgtcgact
     1921 actactcgac tttcgacaac ttcaaggtgg gcgtgctgca ggcggagtcg ctggtggccg
     1981 gcctcaagga gcgcttcccg ggcgtgaaac cctggaacgt ggagctcttc ggcggttcgc
     2041 ccgacgacaa caacgccttc ttcttctacg atggcgccat gtccgttctt cagcccatga
     2101 tcgatgccgg cgagatcgtc gtcccgtcgg gtcagatggg catggacaag gtgggtacgc
     2161 tgcgctggga cggcgcggtg gcccagtccc gcatggacaa cctgctgtcg gccaactaca
     2221 ccgacaagaa ggtccatgcc gtgctgtcgc cctatgacgg cctgtcgcgc ggcatcatct
     2281 cctcgctcaa gggcgtgggc tatgccccgg ggcccgagat gccgatcgtc accggccagg
     2341 acgccgaagt cgcatccgtc aaggcgatgc aggccggcga acagtattcg accgtgttca
     2401 aagacacgcg cgaactggcc aagaccactg ccggcatggt cgacgcggtg atgcagggca
     2461 aggaacctgc catcaacgac acgaagacct acaacaacgg cgtgaaggtg gttccgtcca
     2521 acctgctggt gccctattcg gtgggcaagg acgacatcca gaagatgctg gtcgactccg
     2581 gctacatcaa ggctgaagaa ctgcagtaag catctgatgc tgcgggccct gcaagttgac
     2641 ttgcagggcc cgtctttctt tgtgtaagtc tggatggacc gcccgcgaga aggcgggacg
     2701 cgcgcagccg gacagtcggc ggcgggacat gggaggcgac gggatcggcc atgcagccca
     2761 tccttgagat gcatggaata accaagacgt ttccgggtgt gaaggcgctg acggacgtca
     2821 atctcacggt catgccagcc gagattcatg cggttgtggg cgagaacgga gcgggcaaat
     2881 ccaccctgat gaaggtcctc tccggcgtct atccctccgg cagctacgat ggcatcatca
     2941 aattcgaggg caaggaccgt cacttcaaga ccatcgccga ttccgaggac atcggcatca
     3001 tcatcattca tcaggagctg gctctggtgc cactcttgtc gatcgccgag aacatcttcc
     3061 taggccacga gacggccagg accggcgtga tcaactggtt cgaagccttt tcgaggacca
     3121 gggacctgct gaaaaaggtc ggcctcagcg agcatccgaa cacgctgatc accaacatcg
     3181 gcaccggcaa gcagcagctg gtcgagatcg ccaaggcgct gaacaagaag gtgaagctgc
     3241 tgatcctcga cgagcccacc tcgagcctca acgagacgga ttcgaatgcc ctgctcgatc
     3301 tgctgaaggg cttcaagtcg caaggtatct cgtcgatcct gatcacgcac aagctgaatg
     3361 aggtgatgaa ggttgccgac cgcatcaccg tgatccgtga cggcaagacc atcgacacga
     3421 tggacgttca ttccggcgag gtgaacgagg atcgcatcat caaggccatg gtgggccgcg
     3481 agcggaccga ccgctatccg ccgcgccagg ccaaggtgca ggatacgatc ttcgaagtga
     3541 agaactggaa tgtctttcat cctctgcacg cggaccggca gatcatcaag gacgtcaacc
     3601 ttcatgtgaa gaagggcgaa gtcgtcggca tcgccggcct catgggggcc ggccgcaccg
     3661 aacttgccat gtcgatcttc ggccgcgcct atggccagcg gatcagcggc gaggtgctgc
     3721 tgcacggcag tccggtggat gtctcgacca ttccgaaggc gatggatgcc ggactggctt
     3781 atgccaccga agaccgcaag acttacggcc tcgtgctgat tgaccacatc aagcacaatg
     3841 tcacgctggc taatcttgac ggcgtgtcca gtcgcggcgt gatcgacgac atgcatgagc
     3901 tgactgtcgc caacaagtat cgcaacgacc tccgcattcg ctcggccagc gtcttccagg
     3961 caactgtcaa tctttcgggc ggcaatcagc agaaggtcgt gctgtcgaag tggctgttc
//
LOCUS       DBNGVR010000388         3516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2303517, whole genome shotgun sequence.
ACCESSION   DBNGVR010000388 DBNGVR010000000
VERSION     DBNGVR010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2303517"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1625)
                     /gene="ftsY"
                     /locus_tag="AC16A0_08280"
     CDS             complement(21..1625)
                     /gene="ftsY"
                     /locus_tag="AC16A0_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3105370.1"
                     /translation="MSGFFKKLFNRITGKKEEAAPPPAIEAQPEPEALTEPVVVLEPE
                     PQPQPERQPEPVAVAPSAEPEPAPEAVAEAAPVAPREEPRPAKAKPAVAKTKPKPETA
                     PPKTSPKAKTKVPEPPATKPDAKAKAKAPARSVEPKPATKKAAPKPVRVTQERPKPAP
                     RSAPVPIPEPEELTPPAPEVIAEPPVVALPPEPVAPPAVAPTPAAAEFEPEPPPAVPA
                     APQAAEEARQRRGWFSRLKEGLSKSSKSITGSITSIFTKRKLDKDTLQDLEDVLLQAD
                     LGLPMAERIVRAVSEGRYDKEIDPEEVKRILASEVAKVLKPVEVPFNFGTEKPFIILV
                     VGVNGAGKTTTIGKLGAIASREGFKVMFAACDTFRAAAIEQLTVWGQRIGAKTMARPT
                     GADASGLAFDAIKEAKADGTDIVFIDTAGRLQNKAYLMDELDKIVRVIKKQDETAPHA
                     VLLVLDATTGQNAMAQADIFTKVAGVTGIIMTKLDGTARGGILVAIAEKFKLPVHAIG
                     VGESIDDLQPFEAEGFARAIAGLEDR"
     gene            complement(1622..2869)
                     /gene="mtaB"
                     /locus_tag="AC16A0_08285"
     CDS             complement(1622..2869)
                     /gene="mtaB"
                     /locus_tag="AC16A0_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR3105371.1"
                     /translation="MAETQFVTFGCRLNTYESEVMRGHAEAAGLSDAIVFNTCAVTGE
                     ATRQARQAIRRARAENPAARIVVTGCAAQTDPQMFAAMPEVDKVLGNEEKLRAESWQP
                     GDGPRVAVADIMKVKAATPHLIDGLKERTRAFVQVQNGCDHRCTFCIIPFGRGNSRSL
                     AAEDVVEQIRRLVASGYSEVVLTGVDLTSWGADLDGTPRLGHLVSRILFQVPDLKRLR
                     LSSIDSIEADPELMEVIAGEPRIMPHIHLSLQSGDNLILKRMKRRHAREDSIAFCQAL
                     RSRRPDIVFGADIIAGFPTETDGMFENSLRILDDCSLTYLHVFPFSPRPGTPAARMPQ
                     LDKAVIKARAQRLREAGAARLDSFLAGEVGATRQVLIETEATGRTEHFAQVRFASRMA
                     PGAIVQARVIGRGDGHLEARLAA"
     gene            complement(2873..>3516)
                     /gene="dapF"
                     /locus_tag="AC16A0_08290"
     CDS             complement(2873..>3516)
                     /gene="dapF"
                     /locus_tag="AC16A0_08290"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR3105372.1"
                     /translation="EVESCGNASRCIADLIFKEKKTTRATIDTKGGFLVAEKAGDALV
                     TIDNGAPRFDWKDIPLSEKFHDTRHIELQVGPVEAPLIHSPSVVNVGNPHCIFWVKDL
                     DVVDLSKVGPMLEHHPLFPERANITLARVDAKDHVTIRVWERGAGLTRACGTAACAVM
                     AAGARIRIIGRKATISLPGGDLFMECRESDGHILMTGPVSYDFEGDLPAHLAV"
BASE COUNT          527 a         1103 c         1204 g          682 t
ORIGIN      
        1 ggcccggtct ttcgtatcgc tcatctgtcc tccagcccgg caatggcgcg ggcaaaacct
       61 tcggcctcga agggctgcag gtcgtcgatc gattcgccca cgccgatggc atggacaggc
      121 agcttgaact tctcggcgat ggcgacaagg atgccgcccc gtgcggtgcc gtccagcttg
      181 gtcatgatga tgccggtgac acccgccacc ttggtgaaga tatcggcctg ggccatggca
      241 ttctggccgg tcgtcgcgtc gagcaccagc agcacggcat gcggcgccgt ctcgtcctgc
      301 ttcttgatga cgcggacgat cttgtcgagc tcgtccatca ggtaggcctt gttctgcagc
      361 cggccagcgg tgtcgatgaa gacgatgtcc gtgccatcgg ccttggcctc cttgatggca
      421 tcgaaggcga ggcccgaggc atccgcgccc gtgggccgcg ccatggtctt ggcgccgata
      481 cgctggcccc agacggtcaa ttgttcgatg gctgccgcgc ggaaggtgtc gcaggccgcg
      541 aacatgacct tgaaaccttc gcgcgaggcg atggcaccga gcttgccgat ggtggtggtc
      601 ttgcccgcac cgttcacgcc caccaccaga atgatgaagg gtttctcggt tccgaagttg
      661 aacggcactt cgaccggctt cagcaccttg gccacttcgc tggccagaat gcgtttcacc
      721 tcctccgggt cgatctcctt gtcatagcgg ccctcggaga cggcgcgaac gatccgctcg
      781 gccatgggca gtccgagatc ggcctgcagc agcacgtcct cgagatcctg cagcgtgtcc
      841 ttgtcgagct tgcgcttggt gaagatcgag gtgatcgagc cggtgatcga cttcgatgac
      901 ttggacaggc cttccttgag ccgtgagaac cagccccggc gctggcgcgc ttcttctgcc
      961 gcttgcggcg ccgccgggac agccggcggc ggttccggtt caaactccgc ggccgctggc
     1021 gtcggtgcga cggctggcgg cgctacgggc tcgggaggca acgccaccac cggcggttcg
     1081 gcaatgacct cgggcgccgg cggtgtcagt tcttctggtt caggtatggg gaccggcgcg
     1141 gatcgtggcg cgggcttcgg tctctcctga gtgactcgaa ccggcttcgg cgctgccttc
     1201 ttcgtggcgg gtttcggctc gacgctcctg gcgggcgcct tggcctttgc cttggcgtcc
     1261 ggctttgtcg ctggtggctc ggggactttg gtcttggcct tcggagatgt ctttggcgga
     1321 gccgtctcgg gtttgggctt ggtctttgcg acggccggtt tcgccttcgc tggccgaggt
     1381 tcttcccgcg gcgctacggg tgccgcctcg gccaccgctt caggtgccgg ttccggttcg
     1441 gctgacgggg cgacggccac gggctccggt tgccgctccg gctgtggttg cggctccggt
     1501 tccagcacga caaccggttc ggtaagggct tccggttctg gctgcgcctc gatggccggc
     1561 ggcggtgcgg cctcctcctt cttgccggtg atccggttga agagcttctt gaagaagccg
     1621 ctcatgccgc aagcctggct tcgaggtgac catcgccgcg gccgatgacc cgcgcctgca
     1681 cgatggcgcc gggcgccatg cggcttgcga aacggacctg ggcgaaatgt tcggtgcggc
     1741 ccgtggcttc ggtttcgatc aggacctggc gcgttgcacc tacttcaccg gcgaggaagc
     1801 tgtcgaggcg ggcggcgccg gcttcgcgca ggcgctgtgc ccttgccttg atcactgcct
     1861 tgtcaagctg cggcatgcgg gcggccggag ttcccgggcg gggcgaaaag ggaaagacat
     1921 ggaggtaggt caggctgcag tcgtcgagga tgcgcagcga gttctcgaac atcccgtccg
     1981 tttcggtggg aaagccggcg atgatgtcgg cgccgaagac gatatccggg cggcgggacc
     2041 gcagcgcctg gcagaaggcg atcgagtcct cgcgggcatg acgccgcttc atgcgcttca
     2101 ggatcaggtt gtcgccggac tgcaacgaga gatggatgtg gggcatgatg cgcggttctc
     2161 ccgcgatcac ctccatgagt tcgggatcgg cctcgatcga gtcgatggat gagaggcgca
     2221 ggcgcttgag atcgggcacc tggaaaagaa tgcgcgacac gagatggccg agccggggcg
     2281 tgccgtccag atcggccccc caggaggtga gatcgacgcc ggtgagcacg acctcggaat
     2341 atcccgaagc aacgaggcgc cgtatctgct ccacgacgtc ttccgcggca agcgaacgcg
     2401 aattcccgcg gccaaaggga atgatgcaga aggtgcagcg atggtcgcag ccgttctgca
     2461 cctggacgaa ggcgcgggtg cgctccttca gcccatcgat gagatgcggc gttgccgcct
     2521 tgaccttcat gatgtcggcc accgccacgc gcggtccgtc gcccggctgc cagctttccg
     2581 cccgcagctt ttcctcattg cccaggacct tgtccacctc cggcatggcg gcgaacatct
     2641 gcggatcggt ctgggctgcg cagcctgtca cgacgatgcg ggcggccgga ttttccgcgc
     2701 gggcgcggcg gattgcctgg cgggcctggc gtgtagcctc gccggtcacg gcgcaggtat
     2761 tgaacacaat ggcatcggac aggcccgcgg cctctgcatg gccacgcatg acttccgact
     2821 cataggtatt gagcctgcag ccgaaggtga cgaactgggt ttcggccatg atctagaccg
     2881 ccagatgggc cggaagatcg ccctcgaagt catagctgac cgggccggtc atgaggatgt
     2941 ggccgtcgct ctcgcggcat tccatgaaga gatcgccgcc gggaaggctg atcgtggcct
     3001 tgcggccgat gatgcggatg cgcgcgccgg ccgccataac ggcgcaggcc gccgtaccgc
     3061 aggcgcgggt caggccggca ccgcgttccc agacgcggat cgtcacgtga tccttggcgt
     3121 cgacccgggc aagggtgata ttggcccgct ccgggaagag cggatgatgt tcgagcatgg
     3181 ggccgacctt cgacagatcc acgacatcca aatccttgac ccagaagatg cagtggggat
     3241 tgcccacatt cacaaccgat ggcgaatgga tcagcggcgc ttccaccggg ccgacctgca
     3301 gttcgatgtg gcgcgtgtca tggaacttct ccgagagcgg gatgtccttc cagtcgaacc
     3361 ggggcgcccc gttgtcgatg gttaccaggg cgtcccccgc cttctcggcg acaaggaagc
     3421 cacccttggt gtcgatcgtg gcgcgcgttg tcttcttttc cttgaagatc aggtctgcga
     3481 tgcagcgcga ggcgttgccg cagctttcga cctcgc
//
LOCUS       DBNGVR010000389         2101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3483006, whole genome shotgun sequence.
ACCESSION   DBNGVR010000389 DBNGVR010000000
VERSION     DBNGVR010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3483006"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="AC16A0_08295"
     CDS             complement(<1..1251)
                     /locus_tag="AC16A0_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR3105373.1"
                     /translation="MISGVSPLVAELKARAEAGRPVTIGLAGCGQMGTDIVVQLNRMP
                     GLRLGAVAEMRPPQALAATTLAGFPDEATVHADSAAEIDSAIERGRVAITSNLDALAR
                     AGRIDVVIDATGNPNIGTRFALDAIANGKHVVMLNVEADITIGRHLHEEARRAGVIYT
                     GAAGDEPAATLELIGFAQSIGLDIICAGKGKNNPLKFDAVPAEYEAEARARNMNARML
                     VEFVDGSKTMIEMVAIANCTGLVPDVPGMHGPAATREELASVLVPKADGGVLSKAGCV
                     DYSIGKGVAPGVFCIVGTDHPRVLERLIDLKVGKGPYFTLFRPYHLTSLEVPLSAARA
                     VLHKRADMVPLEQPIAEAITVAKRDLKPGETLGKIGETEYRAWAMTHAAARSANAVPL
                     GLAEGAKVIKPVKAGEYLTYENCVP"
     gene            complement(1251..1991)
                     /locus_tag="AC16A0_08300"
     CDS             complement(1251..1991)
                     /locus_tag="AC16A0_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3105374.1"
                     /translation="MAKLKYLDLREKVIEICLRMNEEGINQGTSGNVSARIDGGFLIT
                     ASGVPYHKMKPHHIVEMDLEGHYIGDYLPSTEWRMHMDIFKHRAEAGAIVHTHSIYAT
                     ALACLRKDIPPFHYMIGIGGGTTIRCSDYAEFGTQALSDVMLKALEDRSACLLGNHGQ
                     IAFGPNLEKALWRAGEVETISHQYWAALQAGTPVLLNEEQMTTVLARFKTYGKQSDEL
                     GKDEAHAVFAPVRIDVPKKDKKKVKKAK"
BASE COUNT          348 a          673 c          660 g          420 t
ORIGIN      
        1 tggcacgcag ttctcgtagg tcaggtactc gccggccttc accggcttga tcaccttggc
       61 gccttcggca aggcccagcg gcacggcatt ggcgctgcgc gccgcggcat gggtcatggc
      121 ccaggcacgg tattcggtct cgccgatctt gcccagcgtc tcgcccggct tgagatcgcg
      181 cttggccacc gtgatggctt cggcgatggg ctgctccagc ggcaccatgt cggcgcgctt
      241 gtgcagcacc gcccgcgccg ctgaaagcgg cacctccagg ctggtgagat gatagggccg
      301 gaacagcgtg aaataggggc ctttgcccac cttgagatcg atcaggcgtt caagcacgcg
      361 cggatgatcg gtcccgacga tgcagaagac gcccggcgcc acgcccttgc cgatggaata
      421 gtcgacgcag cctgctttcg agaggacgcc gccatccgcc ttcggaacca gcaccgaagc
      481 cagttcctcg cgcgttgccg ccggtccatg catgcccgga acatcaggca ccagaccggt
      541 gcagttggcg atcgccacca tctcgatcat ggtctttgaa ccgtcgacga attccaccag
      601 catgcgcgcg ttcatgttgc gcgccctggc ctcggcctcg tattcggccg gcaccgcatc
      661 gaacttcagc ggattgttct tgcccttgcc ggcacagatg atgtcgagcc cgatggactg
      721 ggcgaagccg atcagttcca gcgtcgccgc cggctcgtcg ccggctgccc ccgtatagat
      781 cacgcccgcg cggcgcgcct cttcatgcag gtggcggccg atggtgatgt cggcttcgac
      841 attcagcatc accacgtgct tgccattcgc gatggcatcc agcgcgaagc gcgtgccgat
      901 attgggattg ccggtggcgt cgatgacgac gtcgatgcgg ccggcccggg ccagggcatc
      961 gagattggac gtaatcgcca cccggcctcg ctcgatcgcc gaatcgatct ctgcggcact
     1021 atccgcatgc accgtcgctt cgtcggggaa accggccaga gtcgtggcgg ccagggcctg
     1081 gggcggacgc atttcggcca cggccccgag gcgcaggccc ggcatccggt tcagctggac
     1141 gacgatgtcg gttcccatct ggccgcagcc ggccaacccg atggtcacgg gccgtccggc
     1201 ctcggcacgg gctttcagtt cggcaacgag aggcgagact ccggagatca tcacttggct
     1261 ttcttgacct tcttcttgtc cttcttcggc acatcgatcc gcaccggtgc gaagaccgca
     1321 tgcgcctcgt ccttgccgag ttcgtccgac tgtttaccat aggttttgaa gcgggccagc
     1381 actgttgtca tctgctcttc attcagcagg acgggggttc cggcctgcag cgccgcccag
     1441 tactggtggc tgatggtttc gacctcgccc gcccgccaca gggccttttc gaggttgggg
     1501 ccgaaggcga tctggccgtg attgccgagc aggcaggccg agcggtcctc cagtgccttg
     1561 agcatgacgt cggaaagggc ctgggtaccg aattcggcat agtccgagca gcggattgtg
     1621 gtgccgccgc caatcccgat catgtagtgg aagggcggaa tgtccttgcg cagacaggca
     1681 agcgccgtgg catagatcga gtgcgtatgc acgattgcgc cggcttccgc ccggtgcttg
     1741 aagatatcca tgtgcatgcg ccactcggtg gagggcagat agtcgccgat gtagtgcccc
     1801 tcgagatcca tctcgacgat gtgatgcggc ttcatcttgt gatagggaac gccggatgcg
     1861 gtgatcagga atccgccgtc gatccgtgca ctgacgtttc cggaggtgcc ctggttgatg
     1921 ccttcctcgt tcatgcggag gcagatctcg atgaccttct cgcgcagatc gagatatttg
     1981 agctttgcca tgtcgtcgtc tccaatgcct gaaaatcggc gcggaaccta cagccgtgac
     2041 cggacttcgt ccatgaaaag cttgtctcag ccgggcgtca cataggaacc ccgcacaatt
     2101 t
//
LOCUS       DBNGVR010000390         5493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1912734, whole genome shotgun sequence.
ACCESSION   DBNGVR010000390 DBNGVR010000000
VERSION     DBNGVR010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1912734"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16A0_08305"
     CDS             complement(<1..344)
                     /locus_tag="AC16A0_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105375.1"
                     /translation="MPATGSDTKRHTMWFTAAALCALLAVTGAAPPATAEGVPLDNAG
                     IRSMVTGKRIYLATPMGGELPLYYRANGRVDGTGEAVGLGKYLKPKDSGRWWVANNKL
                     CQKWQEWYDGRT"
     gene            383..961
                     /locus_tag="AC16A0_08310"
     CDS             383..961
                     /locus_tag="AC16A0_08310"
                     /inference="COORDINATES: protein motif:HMM:NF022537.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HIR3105376.1"
                     /translation="MSINRFGSLQFAGMVANFLLFEVAWFAAVSGGAGGWPWLGSLPA
                     LLVVALHLAVNAGRLAQEAFLIAGITLLGVLVEGGFLASGIITYAGTEAGQWAPPIWI
                     IALWFGFGTLPHASLGWLSGRWVLQALLGAICGPLSYVAGEALGAAQIAEPRLWSIGV
                     IGVAWAVALPLMFVMADALARWPRLASHALQD"
     gene            989..1882
                     /locus_tag="AC16A0_08315"
     CDS             989..1882
                     /locus_tag="AC16A0_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3105377.1"
                     /translation="MKLVSYNIQYGFGSDGRYDLERIARIVDGADVIALQEVERFWKR
                     SGLDDQPAILAGLLPRYHQVYGPAFDMDASAVAAGRVINRRRQFGTMLLSLRPIRWSR
                     LHVLPMRRMIDPLNTRNAALECLIETDCGPIRFLSLHLAHVGVAERLEQIDYLLQQHD
                     RAATDGGPWSGRDDEPARNWTNDEPEPASPAAAIWLGDFNCEPGSAEYLRIVGQTPYH
                     PGARYAGGLSDAMTRDPAAPGEVYTHVKMIAGVLRRRQLDHCFVSSALKPRVRSVHAA
                     NGEVASDHHPLWIDIDLESAA"
     gene            1879..2721
                     /locus_tag="AC16A0_08320"
     CDS             1879..2721
                     /locus_tag="AC16A0_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3105378.1"
                     /translation="MSLTVFLAVIAAAAMHAAWNALVKVRTDRFASISITALGMALVA
                     VPVLPFVDVPHAAVWPWIVASVIIHVGYRLFLIMAYNAGDLAQTYPLARGAAPLMTAV
                     GAIFLVHEVPGPIAILGILLLSGGTLLMSLRGGLLGQLNGRAVGYALATSVFISGYTL
                     VDGNGARLASTASSYAAWLFLCDGLCSMVIGFYYRGKKLLHVIAREWKTGLMAGALSA
                     GSYWIAMWAMTRAPIASVAALRETSIVFAMIISVFVIGERMTAWRITAAACIVAGAIA
                     LRLA"
     gene            2782..4290
                     /gene="betC"
                     /locus_tag="AC16A0_08325"
     CDS             2782..4290
                     /gene="betC"
                     /locus_tag="AC16A0_08325"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017958041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HIR3105379.1"
                     /translation="MTRPNILVLMVDQLNGTLFPDGPAPWLHAPHLRRLAERSVRFRN
                     AYTGSPLCAPGRASFMSGQLPFRTRVYDNAAEFPSDTPTFAHHLRRAGYRTTLSGKMH
                     FVGPDQLHGFEERLTTDIYPADFGWTPDYRKPGERIDWWYHNLGSVTGAGVAEITNQL
                     EYDDDVAHQAIAKIYDLSRGADARPWCLTVSFTHPHDPYVARRKYWDLYNDCEHLLPP
                     GRSIPYDQQDPHSQRLMDACDWRAFDITETQIRRARQGYFANISYVDDKIGEILAALT
                     ASRQDAIVLFLSDHGDMLGERGLWFKMNFFEGSARVPLMVAAPGLAPGRIDTPVSTID
                     VTPTLCDLAGISMIDVLAWTDGESLVPLAQGMARQSPVAMEYAAEGSWAPLVALRKGK
                     WKYIRCALDPDLLFDLGTDPAERVNRAGDPAARAILDEMRSIADARWDLAAYDADVRA
                     SQARRWVVYEALRNGSYFPWDYQPLQKASERYMRNHMDLNILEESKRFPRGE"
     gene            complement(4293..5072)
                     /locus_tag="AC16A0_08330"
     CDS             complement(4293..5072)
                     /locus_tag="AC16A0_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3105380.1"
                     /translation="MLETGDRRITLAYDGPVAVITLARPDKLNALDYEMVLALERAAH
                     AIDARPEIRVAIITGLGDRSFCAGGDIEAWSAHTPEDFGMSWVRHGHRAFDALARLRQ
                     PLIAALNGHALGGGLELAATADFRIAEEHVKLGSPETGLGIIPGWSGTQRTVRRFGSQ
                     LVRRMAVLGEVFTAAEALSLGLVDAIAAKGQSLEAARDMAARIAVRSPSATQATKMLI
                     NAAEGEESERVLEAIAGTMAAASADLKEGVAAFREKRKPGF"
     gene            complement(5065..>5493)
                     /locus_tag="AC16A0_08335"
     CDS             complement(5065..>5493)
                     /locus_tag="AC16A0_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003573736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HIR3105381.1"
                     /translation="VSKLGVRPHVTAGAGGFVDITSRARKIVFSGFYNAGAKMEIVDG
                     KLRIDKEGKVRKLVKDVEQISFSGRRGVKQAQDVTYVTERCVLKLTPEGIVVTEIAPG
                     VELQANILDQAEFPLIVSPALKQMEAGLFRPDVMGMKLDA"
BASE COUNT          854 a         1891 c         1742 g         1006 t
ORIGIN      
        1 aaggtgcgcc cgtcgtacca ctcctgccac ttctggcaga gcttgttgtt cgccacccac
       61 cagcggccgc tgtcctttgg tttcagatac ttgcccagcc cgaccgcctc gccggtcccg
      121 tcaacccgtc cattggcccg gtagtagagc ggaagctcgc cgcccatcgg tgtcgccaga
      181 tagatgcgct tgcccgtgac catcgaccgg atgccggcat tgtcgagcgg caccccttcc
      241 gccgtggccg gcggcgccgc gccagtcact gccaagaggg cgcacagcgc cgccgctgta
      301 aaccacatgg tgtgccgctt cgtatccgat ccggttgccg gcattcgttc ctccatcaag
      361 ataattcagg gccattacgc aggtgagcat caatcggttc ggttcgctgc agttcgccgg
      421 catggtggcg aacttcctgc tcttcgaagt cgcgtggttc gccgccgtaa gcggcggcgc
      481 gggcggctgg ccttggctgg ggtcgttacc cgccctgctg gtggtggccc tgcatctggc
      541 cgtcaatgcc ggtcgccttg cccaggaggc gttcctgatt gccggcatca ccctgctcgg
      601 cgtcctggtg gagggcgggt ttctggccag cggcatcatc acatatgccg gcaccgaagc
      661 cggccagtgg gctccgccaa tctggatcat tgcgctctgg ttcggcttcg gcaccctgcc
      721 gcatgcgagc cttggctggc tgtcgggccg ctgggtgctc caggccctgc tcggtgccat
      781 ctgcggcccg ctcagctacg tggcgggcga agccctgggc gcggcgcaga tcgccgagcc
      841 gcgcctgtgg tccatcggcg tcatcggagt ggcctgggcc gtcgcgcttc ccctgatgtt
      901 cgtcatggcc gacgcgctcg cacgctggcc gcggcttgcc agccacgcct tgcaggacta
      961 agcacgcgcc cgggggcggg gcgttggcgt gaagctggtc agctacaaca ttcaatatgg
     1021 cttcggcagc gacggccgct atgacctcga acgcatcgcg cgcatcgtcg atggcgccga
     1081 tgtcatcgcc ctgcaggagg tcgagcgttt ctggaagcgg tcggggctcg acgaccagcc
     1141 ggccatcctg gccgggcttc tgccgcgcta tcaccaggtc tatggcccag ccttcgacat
     1201 ggatgcgagc gcggtcgcgg cggggcgcgt gatcaaccgg cggcggcagt tcggcaccat
     1261 gctgctgtcg ctgcgcccga ttcggtggtc caggctccat gtcctgccga tgcgccgcat
     1321 gatcgacccg ctcaacacac gcaacgcggc cctggaatgc ctgatcgaaa cggactgcgg
     1381 gcccatccgc ttcctgtcgc tgcatctcgc ccatgtcggc gtcgccgaac ggctggaaca
     1441 gatcgactac ctgcttcagc agcatgaccg cgccgcaacg gatggcggcc catggagcgg
     1501 aagggacgac gagcccgcgc gaaactggac caacgatgag cctgaacccg cgtctccggc
     1561 agccgcaatc tggcttggcg acttcaattg cgagcccggc agtgccgagt atctgaggat
     1621 cgttggccag actccctatc atccgggcgc gcgctacgcc ggaggcctca gcgatgccat
     1681 gacgcgcgat cccgccgccc caggcgaagt ctacacgcat gtgaagatga tcgccggagt
     1741 cctgcgccgg cgccagctgg atcactgctt tgtcagctcc gcgctcaagc cgcgcgtcag
     1801 gtcggtgcac gccgcgaatg gcgaagtcgc ttcagaccat cacccgctgt ggatcgacat
     1861 cgatctcgag tccgcggcat gagtctcacc gtctttctcg cggtgatcgc agcggcggcc
     1921 atgcatgccg cgtggaatgc gctggtcaag gtgcgtaccg accgcttcgc ctcgatctcg
     1981 atcaccgcgc ttggcatggc gctcgtcgcc gttccggtct tgcccttcgt ggatgtcccc
     2041 catgctgcgg tctggccgtg gatcgtcgcc tcggtcatca tccatgtagg ttacaggctc
     2101 tttctgatca tggcctacaa tgccggcgac ctggcgcaga cctatccgct ggcgcgcggt
     2161 gccgcccccc tgatgacggc agtgggcgcg atcttcctcg ttcacgaggt tcccggcccc
     2221 atcgcaatcc tggggatact gctgctgtcc gggggcacgt tgctgatgtc gctgcgcggc
     2281 gggctgctgg gccaactcaa cggccgggcc gtgggctatg cgcttgccac gtcggtgttc
     2341 atctcgggct atacgctcgt cgatggcaat ggcgcgcgcc ttgccagcac ggcctccagc
     2401 tatgcggcat ggctcttcct ctgcgatggc ctctgttcga tggtgatcgg cttctactac
     2461 cgcgggaaga agctgctgca tgtgatcgcc cgcgagtgga agaccggcct catggccggc
     2521 gcgctgagtg ccggatccta ctggatcgcc atgtgggcca tgacgcgtgc cccgatcgct
     2581 tccgtcgcgg ccctgcgcga aacctcgatc gtcttcgcga tgatcatctc ggtcttcgtg
     2641 atcggcgagc gaatgaccgc ctggcgcata acggccgcgg cctgcatcgt ggccggagcc
     2701 atcgcgctcc ggctggctta gcccttgcgg ggcggcaagg ccgggagtag ctcacagcca
     2761 cagccgcaaa ggatgcccgc cgtgacccgc cccaacattc tcgttctgat ggtcgaccag
     2821 ctgaacggca cgctcttccc cgatggtccg gcgccatggc tgcatgcacc ccatctccgc
     2881 aggcttgccg agcggtcggt gaggttccgc aatgcctata cgggttcgcc gctctgcgcg
     2941 ccgggccgcg ccagcttcat gtcggggcaa ttgccgttcc ggacccgcgt ctatgacaat
     3001 gcggcggagt tcccctccga cacgcctacc ttcgcgcatc acctgcgccg cgcggggtac
     3061 cgcacgacgc tctcaggcaa gatgcatttc gtcggacccg atcaactgca cggcttcgag
     3121 gaacgcctga ccaccgacat ctatccggcg gatttcggct ggacgcccga ttaccgcaag
     3181 ccgggcgaac gcatcgactg gtggtaccac aacctcggat ccgtgacggg cgccggtgtc
     3241 gccgagatca ccaatcagct cgaatacgat gacgatgtcg cgcatcaggc cattgcaaag
     3301 atctatgatc tctcgcgggg cgccgatgcg cggccctggt gcctgacggt gagtttcacc
     3361 catccgcacg atccctatgt cgcccgccgc aagtactggg atctctacaa tgactgcgaa
     3421 cacctgctgc cgcccgggcg cagcatccca tacgaccagc aggatccgca ttcgcagcgc
     3481 ctgatggatg cctgcgactg gcgggccttc gacatcaccg agacgcagat ccgccgcgcg
     3541 cgccagggct acttcgccaa catctcctat gtcgatgaca agatcggcga gatcctcgcg
     3601 gccctgacgg cttcgcggca ggacgccatc gtgcttttcc tctcggacca cggcgacatg
     3661 ctgggcgagc gcggcctctg gttcaagatg aacttcttcg agggttcggc gcgcgtaccc
     3721 ctgatggtgg cggcgccagg gctggcgcca ggccgcatcg atacgccggt ctcgaccatc
     3781 gacgtgactc cgaccctctg cgatctcgcc ggcatctcga tgatcgacgt gttggcctgg
     3841 acggacggcg aaagcctcgt ccccctggcg caaggcatgg cgcggcagtc gccggtcgcc
     3901 atggaatatg ctgcggaggg ctcctgggcg ccgctcgttg ccctgcgcaa gggcaaatgg
     3961 aaatacatcc gctgcgcgct cgatccggac ctgctcttcg accttggaac cgatccggcg
     4021 gagcgcgtca accgcgctgg cgatcccgcg gcccgcgcca tcctggatga gatgagaagc
     4081 attgccgacg cccgctggga tctcgccgcc tatgatgccg atgtccgcgc cagccaggca
     4141 cggcgctggg tggtctacga ggccctgcgc aacggaagct actttccgtg ggattaccag
     4201 ccgctgcaga aggccagcga gcgctacatg cgcaatcaca tggacctgaa catcctcgag
     4261 gaaagcaagc gcttcccgcg cggcgagtga cgtcagaagc cgggtttgcg cttttcgcgg
     4321 aaggcggcga cgccttcctt cagatcggca ctggcagccg ccatggtacc ggctatggct
     4381 tccagcaccc gctccgactc ctcgccttcc gccgcgttga tcagcatctt cgtcgcctgc
     4441 gtcgccgaag ggctgcgcac cgcgatgcgc gcggccatgt cgcgcgcggc ctccagagac
     4501 tggcccttcg ccgcaatcgc gtcgacgagc ccgaggctca aggcctccgc ggccgtgaag
     4561 acttcgccca gcacggccat gcggcgcacg agctgcgatc cgaagcggcg cactgtgcgc
     4621 tgcgtgccgg accaacccgg aatgatgccg agtccggtct cgggcgaccc tagcttcaca
     4681 tgctcttcgg cgatgcggaa atcggccgtc gccgcaagtt cgaggccgcc gcccagggca
     4741 tggccgttga gcgcagcgat caggggctgg cgcagccggg cgagggcgtc gaaggcacga
     4801 tggccatggc gaacccagga catgccgaaa tcctccggcg tatgcgcgga ccaggcctcg
     4861 atgtcgccac cggcacagaa gctccggtcg cccaggcccg tgatgatggc aacccggatc
     4921 tccggacgag cgtcgattgc atgcgctgcg cgttccagcg ccaggaccat ctcgtagtcg
     4981 agcgcgttga gcttgtcggg ccgggccagc gtgatgacgg caaccggccc gtcgtacgcg
     5041 agggtaatgc ggcgatcgcc tgtctcaagc atcgagcttc atccccatga cgtcgggccg
     5101 gaacagccct gcttccattt gcttcagcgc gggcgagacg atcagcggga attcggcctg
     5161 gtcgaggatg ttggcctgca gctccacgcc gggggcgatc tcggtgacga caatgccttc
     5221 gggcgtgagc ttcagcacgc agcgctcggt cacgtaggtc acgtcctgcg cctgcttcac
     5281 gccgcggcgg ccggagaagg aaatctgctc cacgtccttc accagtttcc tgaccttgcc
     5341 ttccttgtcg atgcgcagct tgccgtcgac gatctccatt ttcgcgccgg cattgtagaa
     5401 accggaaaag acgatcttcc tcgcccgtga ggtgatgtcg acgaagccac ccgcacccgc
     5461 cgtcacgtgg ggcctcacgc caagcttcga cac
//
LOCUS       DBNGVR010000391         3189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3287984, whole genome shotgun sequence.
ACCESSION   DBNGVR010000391 DBNGVR010000000
VERSION     DBNGVR010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3287984"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="AC16A0_08340"
     CDS             complement(<1..406)
                     /locus_tag="AC16A0_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3105382.1"
                     /translation="MTMVLDRRPRQAKRPGDGEGSVQVHLDPNVQIGTAKVFSIYGKG
                     GIGKSTTSSNLSVAFSKLGKRVLQIGCDPKHDSTFTLTKSLIPTVIDILEQVDFHSEE
                     LRPEDFVFQGYNGVMCIEAGGPPAGTGCGGYVV"
     gene            complement(403..>3189)
                     /locus_tag="AC16A0_08345"
     CDS             complement(403..>3189)
                     /locus_tag="AC16A0_08345"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HIR3105383.1"
                     /translation="VIAALESRGLKVIPAFATGLDQRPAIEKFFMKNGSTAIDALVSL
                     SGFSLVGGPAYNDARAAEEVLAALDVPYVAAQPVEFQTLEEWEASERGLHPIETTMMI
                     AIPEIDGATGSIVFGGRYGAEGGERSMRVHAERAAMLATRVSKLVALRQAEKSDRRVA
                     VVLFNFPPNGGSTGTAAFLGVFESLFNTLQALKRDGYAVEVPATVDALRDRILKGNAE
                     RFGTDANVHCRIPADDHVRREPHLAEIEAQWGPAPGRQQSDSGHIHVLGERFGNVFVG
                     IQPAFGYEGDPMRLLFERNFSPTHAFSAFYRYLREDFGAHAVLHFGTHGALEFMPGKQ
                     SGMSQACWPDRLIGDLPNLYLYAANNPSEGTIAKRRAGATLISYLTPPLAQAGLYRGL
                     VELKSSIERWRGLSPDASERDQLAVLVQAQAAAVDLSQAEPAWTDADASIQALQTAIL
                     ELEYTLIPHGLHVVGEAMTAEARRDMLASVAETNPEADMAKMEELLSQDHEIGGLLHA
                     LDGGFIRPVAGGDLIRTPSILPTGRNLHGFDPFRIPSAFAVADGARQADLLLRTHVAN
                     GKPTPETVALVLWGTDNLKSEGGPIAQAMSLLGAVPRFDSFGRLAGASLLPAETLSHP
                     RIDVMLTLSGIFRDLLPLQTRMLAEACYLAAAADEPDNINFVRKHALAHMRKTGCDLE
                     TAALRVFSNADGAYGSNVNMMIDSGRWEDESELGDVFSKRKCFAHGRNGQAAAQSEIM
                     AAVLSGVDLAYQNLESMELGVTTVDHYFDSLGGISRAAEKQKGEAIPVYIGDQTRGAG
                     VVRTLADQVALESRTRVLNPKWYEGMLKHGHEGVRHIECHLTNTVGWSATTGQVAPWV
                     YQQFTQTYVLDQVMRDRLAALNPKAAVKVVNRLLEAERRSFWKPDAETLDALKRAGEE
                     LEDRLEGIGAEVAA"
BASE COUNT          552 a         1011 c         1059 g          567 t
ORIGIN      
        1 ccacgacgta gccgccgcaa cccgtgccgg ccggcggtcc gccggcctcg atgcacatga
       61 caccgttgta gccctggaac acgaagtcct cggggcgcag ttcctcggaa tggaaatcga
      121 cctgttcgag gatgtcgatc acggtcggga tcagcgactt ggtgagcgtg aaggtggagt
      181 cgtgcttggg atcgcaaccg atctgcagca cgcgcttgcc aagcttcgag aaagccaccg
      241 agaggttcga cgatgtcgtg ctcttgccga tgccgccctt gccgtagatg gaaaagactt
      301 tggccgtgcc gatctgcacg ttcgggtcga ggtgaacctg cacgctgcct tcaccgtcac
      361 cgggtcgttt ggcctggcgt ggccggcgat caagaaccat ggtcatgccg caacctccgc
      421 accaatgcct tcgagcctgt cttcgagttc ctcaccggcg cgcttcagcg cgtccagcgt
      481 ttcggcgtcc ggcttccaga aggagcggcg ctccgcctcc agcaaccggt tcaccacttt
      541 cacggccgcc ttgggattga gggcggcaag gcgatcccgc atcacctggt ccagcacata
      601 ggtctgggta aactgttggt agacccatgg cgccacctga cccgtcgttg ccgaccagcc
      661 caccgtattg gtgaggtggc attcgatgtg gcggacgcct tcgtggccat gcttcagcat
      721 gccttcgtac cacttggggt tcagaacccg ggtgcgcgat tcaagcgcga cttggtcggc
      781 cagcgtgcgc acgacgccgg caccgcgagt ctggtctccg atgtacacag gaatggcttc
      841 acccttttgc ttttcggctg cacggctgat tccgcccagg gagtcgaagt agtggtcgac
      901 tgtggtgacc cccagttcca tggactcgag gttctggtag gcaagatcga ctccggaaag
      961 caccgcggcc atgatctcgg actgcgcggc ggcctgtccg ttgcggccat gtgcaaagca
     1021 cttgcgcttg gagaagacgt cgccaagttc gctctcgtct tcccagcggc cgctgtcgat
     1081 catcatgttg acgttcgagc cataggcgcc atccgcattg ctgaacacgc gcagcgcagc
     1141 cgtttccagg tcgcagccgg tcttgcgcat gtgtgcgagg gcgtgcttgc gaacgaagtt
     1201 gatgttgtcc ggttcgtccg cggcggcagc gagatagcag gcctccgcca gcatgcgggt
     1261 ctggaggggc agcaggtcgc ggaagatgcc agagagcgtc agcatcacgt cgatgcgcgg
     1321 atggctgagc gtctcggccg gcagcaggct ggcgccagcc agccgcccga agctgtcgaa
     1381 gcgcggaacg gcccccagca gcgacatggc ctgggcaatc ggcccgccct cgctcttgag
     1441 gttgtcggtg ccccacaaga caagcgccac ggtctcggga gttggcttgc cattggccac
     1501 atgggtcctg agcagcagat cggcctggcg cgcaccgtcc gccacggcaa atgccgatgg
     1561 aatgcggaac gggtcgaagc cgtgcagatt gcggcccgtg ggcaggatgg agggcgtgcg
     1621 gatcaggtcg ccgccggcaa ccgggcggat gaatccgcca tcgagcgcat ggagcaatcc
     1681 gccgatctcg tgatcctgcg agaggagttc ctccatcttc gccatgtcgg cctcgggatt
     1741 ggtttccgcg acgctggcca gcatgtccct gcgggcctcg gccgtcatcg cctcgcccac
     1801 cacatggagc ccgtgcggaa tgagcgtgta ctcgagttcc agaatggccg tctgcagcgc
     1861 ctggatgctg gcatctgcat cggtccatgc gggctcggcc tggctcaagt cgacggcggc
     1921 agcctgcgcc tgaaccagca cggcgagctg atcgcgttcg gaagcatcgg gcgagaggcc
     1981 gcgccagcgt tcgatggacg acttgagttc gacgagcccc cggtagagcc cggcctgggc
     2041 cagcggcggc gtcaggtagc tgatcagtgt ggctccggcg cgccgcttgg cgatggttcc
     2101 ttccgacgga ttgttggcgg catagagata gaggtttggc aggtcgccga tcagccggtc
     2161 gggccagcag gcctgcgaca tgccggactg cttgccgggc atgaactcca gggcgccatg
     2221 cgtgccgaaa tgcagcaccg catgggcgcc gaaatcctcg cggaggtaac ggtagaaggc
     2281 cgagaaggcg tgggtgggcg agaagttgcg ctcgaagagc agccgcatcg ggtcgccttc
     2341 gtaaccgaag gctggctgga tgccgacgaa cacattgccg aagcgctcgc ccaaaacatg
     2401 gatgtggccg ctgtcgcttt gctgccggcc gggggcaggg ccccactgcg cctcgatctc
     2461 cgccagatgg ggctcgcgac gcacgtggtc gtcggccggg atgcggcaat ggacattggc
     2521 gtccgtcccg aagcgctcgg cattgccctt gagaatgcga tcgcgcagcg catccacagt
     2581 ggcaggcacc tcgaccgcgt agccatcgcg cttcagggcc tgcaaggtat tgaaaagcga
     2641 ctcgaagacg ccgaggaaag ccgccgtgcc ggtgcttccg ccgttgggcg ggaagttgaa
     2701 cagcacaacc gcgacccggc gatcactctt ctcggcctgg cggagcgcaa cgagcttgga
     2761 aacccgcgtg gcaagcatcg ccgcgcgctc ggcatggacc ctcatgctac gttcgccgcc
     2821 ttcagcacca taacgaccgc caaagacgat ggaaccggtg gcaccgtcga tctccggaat
     2881 ggcgatcatc atggtcgtct cgatcggatg gaggccgcgt tcggaggctt cccattcttc
     2941 cagcgtctgg aactcgaccg gctgggctgc gacgtaaggc acatccaggg cggccagcac
     3001 ctcttcggcc gcgcgcgcgt cattgtaggc cgggccgccg accagcgaga aaccgctgag
     3061 cgagaccagt gcgtcgatgg ccgtgctgcc gttcttcatg aagaacttct cgatggccgg
     3121 acgctggtcc aggcccgtcg caaaggccgg aatgaccttc agcccgcgcg actcgagcgc
     3181 tgcaatcac
//
LOCUS       DBNGVR010000392         2568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4268158, whole genome shotgun sequence.
ACCESSION   DBNGVR010000392 DBNGVR010000000
VERSION     DBNGVR010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4268158"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="AC16A0_08350"
     CDS             <1..268
                     /locus_tag="AC16A0_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR3105384.1"
                     /translation="GANHPVKDFTTSKVEIVSMNHGFAVDRSSLPAGVVETHVSLFDG
                     SNAGIALEGRPVFSVQHHPEASPGPQDSHYLFRRFVDLMARARG"
     gene            268..1761
                     /locus_tag="AC16A0_08355"
     CDS             268..1761
                     /locus_tag="AC16A0_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3105385.1"
                     /translation="MSFARLDELCRKLEALGHAEAMLQVDEAVSMPAGGGEKRAEAMG
                     TLAALAHDLATAPAVDDWIAAARTKDLDPSQQSALREFERTHRNRTCLSADFVSRKVN
                     AQIRCEQLWRSLRAKNDWDGFLPAFEAIVALAREEAQLRAAALGLSPYDALVEQFDPG
                     SRSAEIATVFADLKRFLTGFIPEALEAQARRRAARPAKPFHGDFPPERQKMLGVTLMT
                     AVGFDFDHGRLDISHHPFCSGVPSDVRMTTRYTTHEFLSSLMGILHETGHGLYEQGLP
                     AEWQHWPSGRARGMAMHESQSLFQEMQISRTREFWSFALPLARDHLGTEQLAGFEVDD
                     LIAHVQKIEKGYIRVDADEVTYPLHVILRFEIEKDLIEGRLAAKDVPEAWDAKMRDYL
                     GLSTIDNPKDGPMQDVHWPSGAFGYFPSYTLGALIAAQLRAAMARDMPDLGAQIGRGD
                     FAAINDWRRRKIWQKASMASTDAILREATGEGLSARHFEDHVKRRYL"
     gene            complement(1765..>2568)
                     /locus_tag="AC16A0_08360"
     CDS             complement(1765..>2568)
                     /locus_tag="AC16A0_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007826881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105386.1"
                     /translation="RVYAVLIYIFLYAPIALIVAGSFNAGKHAMIWQGFSLEWYGKAF
                     SNPLITEALTTSLFIALVTAALSSVFGTLAALGLERMSGWLRNVFDALIYIAIMVPGI
                     VIGISTLIAFVSVFDMVNPWLTATWNWRLEMGTWSVIAAHVLFNIAVVALLVRARLQG
                     MDRSLVEASEDLYATPLGTFRQVVLPLLAPSILAGFLLAFTFSFEDFIIAYFVAGPDV
                     TLPIYVFSSIRRGVTPEINAVGTVVLMTSLALLVVAQLILQRGQRTARA"
BASE COUNT          488 a          848 c          837 g          395 t
ORIGIN      
        1 cggcgccaat catccggtga aggacttcac caccagcaag gtggagatcg tgtcgatgaa
       61 ccatggcttc gcggtggacc gctcttcgct tcctgccggc gtggtcgaaa cccatgtctc
      121 gctcttcgac ggatcgaacg ccggcattgc cctggaagga cggccggtct tctcggtgca
      181 gcaccatccg gaagcctcgc ccggcccgca ggacagccac tatctgttcc gccgcttcgt
      241 cgacctgatg gccagggcgc gcggctgatg tcctttgcaa ggctggatga actctgtcgc
      301 aagctcgagg cgctcggcca tgccgaggcc atgctgcagg tcgacgaggc cgtcagcatg
      361 ccagccggcg gcggcgagaa acgggccgag gccatgggta cgctggcggc cctcgcccac
      421 gaccttgcaa ccgcacccgc ggtcgacgac tggatcgcgg cggcgaggac caaggacctt
      481 gacccctcac agcagtccgc gctccgcgag ttcgaacgca cgcatcgcaa ccgcacctgc
      541 ctctcggccg atttcgtcag ccgcaaggtc aatgcgcaga tccgctgcga acagctgtgg
      601 cggagcctgc gcgccaaaaa cgactgggac ggctttctgc cagccttcga ggcgatcgtc
      661 gcccttgccc gcgaggaggc gcagctccgc gccgcagcgc tcggtctctc gccctatgat
      721 gcgctggtcg aacagttcga tccgggcagc cgttcggccg agatcgcgac cgtcttcgcc
      781 gacctcaagc gcttcctgac cggcttcatt cccgaagcgc tggaggcaca ggcgcgccgc
      841 cgcgctgccc gcccggcgaa gcctttccac ggtgacttcc cgcccgaacg ccagaagatg
      901 ctgggcgtga cactgatgac ggccgtggga ttcgacttcg accatggacg gctggacatc
      961 tcgcatcatc ccttctgcag cggcgtgccg agcgatgtcc gcatgaccac gcgctatacg
     1021 acgcacgagt tcctgtcttc gctcatgggc atcctgcacg agaccggcca cgggctctac
     1081 gaacagggac tgccggcgga gtggcagcac tggcccagcg gcagggcccg cggcatggcc
     1141 atgcatgaaa gccaaagcct gttccaggag atgcagatct cgcgcacgcg cgagttctgg
     1201 agcttcgccc tgccgctggc gcgcgatcat ctgggcacgg agcaacttgc cggcttcgag
     1261 gtcgacgacc tcatcgcgca tgtgcagaag atcgagaagg gctatatccg cgtcgatgcc
     1321 gacgaggtga cctatccgct gcacgtgatc ctgcgcttcg agatcgagaa ggacctgatc
     1381 gagggccgtc tcgcggcgaa ggacgtgccc gaggcctggg atgccaagat gcgcgactac
     1441 ctggggctgt cgaccatcga caatccgaag gacggaccca tgcaggacgt gcactggccg
     1501 tcgggcgcct tcggctattt cccgagctat acgctgggag ccctgatcgc ggcgcagctg
     1561 cgcgccgcca tggcgcgcga catgcccgac ctcggcgcgc agatcggccg cggcgacttc
     1621 gcggccatca acgactggcg ccgccgcaag atatggcaga aggcctcgat ggcctcgacc
     1681 gatgccatcc tgcgcgaggc caccggcgaa ggcctttcgg cccggcactt cgaggaccac
     1741 gtgaagcggc gctatctcta aggatcaggc gcgggccgtc cgctgcccgc gctgcaggat
     1801 gagctgcgcc acgaccagga gcgccagcga tgtcatcagc accacggtgc ccaccgcgtt
     1861 gatctccggc gtcacgccac ggcggatcga ggagaacaca tagatgggca gcgtcacgtc
     1921 cggccccgcc acgaaatagg cgatgatgaa atcctcgaag gagaaggtga aggcaagcag
     1981 gaagcctgcg aggatggagg gcgccagcag cggtagcacc acctggcgaa aggttccaag
     2041 tggcgtggcg tagagatctt ccgaggcttc gacgaggctg cggtccatgc cctgcaggcg
     2101 cgcccgcacc agcagcgcca ccaccgcgat gttgaacagc acatgcgcgg cgatcaccga
     2161 ccaggtgccc atctcgagcc gccagttcca ggtggcggtg agccagggat tgaccatgtc
     2221 gaagacgctg acgaaggcga tcagcgtgga aatgccgatc acgatgccgg gcaccatgat
     2281 ggcgatgtag atcagcgcat cgaagacgtt acgcaaccag ccgctcatcc gctcgaggcc
     2341 gagtgcggcc agtgtgccga agaccgagga cagcgccgcc gtgaccagcg cgatgaacag
     2401 gctggtggtc agcgcctcgg tgatcagcgg attggaaaag gccttgccgt accattccag
     2461 cgagaagccc tgccagatca tcgcatgctt gcccgcattg aaggacccgg cgacgatcag
     2521 ggcgatgggc gcatagagga agatatagat gaggacggca tagacgcg
//
LOCUS       DBNGVR010000393         3109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2108744, whole genome shotgun sequence.
ACCESSION   DBNGVR010000393 DBNGVR010000000
VERSION     DBNGVR010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2108744"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC16A0_08365"
     CDS             complement(<1..611)
                     /locus_tag="AC16A0_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014409.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR3105387.1"
                     /translation="MIENQNTIVVIPARMASTRLPGKPLADIHGLPMILHVWKRAVEA
                     NVGQVLVAAAEIEIAEAVRQHGGDAIVTDPAHPSGSDRIAEALQLRDPEGRFSFVVNL
                     QGDLPTIDPLSVQRCLAGLVNEQADISTIAARIEDQADVDNPNIVKAIAPLSAEREVA
                     FARDFLRQVGPDHPPPHWHHIGVYAYRRPVLERFVSLPVSEREA"
     gene            728..1285
                     /locus_tag="AC16A0_08370"
     CDS             728..1285
                     /locus_tag="AC16A0_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3105388.1"
                     /translation="MDSFEFNKIAGAVLGTALMVFGLHELAGIIYHPHEPEKPGFVVA
                     VPEGEAPAGGETAAAEPEAPAESLGALLAAADPAKGQAGFKACAACHDVTKGGPNKVG
                     PNLWGVVGAMHGVHAGFQYSDAMAAKSGEPWTYEALNEFLTAPAKAIPGTKMAFGGVK
                     KAQDRANLLAYLATLSDAPVPFPAP"
     gene            1408..>3109
                     /locus_tag="AC16A0_08375"
     CDS             1408..>3109
                     /locus_tag="AC16A0_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3105389.1"
                     /translation="MTLSRRSLLKLAGLSPLAKIAGIQLASAEDRDFRHALTLFSDIK
                     YGPDFKHFDYVNPDAPKMGRVRFGIVGSFDSLNPYTFKGDPGGAVVNDALLVSSLDEA
                     STEYGLVASAVWHPEDRSMVIYRLRPEARFHDGKPMTPEDVIWSMQSLREAHPFYNAY
                     YKNISKAEQTGEHEVTFMFSETGNRELPLITGQMPVLPKHWWTGKDAKGRQRDLKETT
                     LEVPLGSGAYVATEIKPGASIRMKRVADYWGRDLPVNAGQDNFEEMEYIYFRDANVAL
                     EAFAGDQYDWRLENSSKSWATGYDFPAIKDGRVIKEEITLKQVEGMQSWAMNIRRPKF
                     QDVRVRQALNYAFDFEWANTNLFYGQYKRSRSYFNNSEMEAKGLPSPEELKLLEPLRD
                     QLPPEVFTAEFANPVNDTPQNRRKNLREASRLLDQAGWKVTQDGNRSLLKNDKGEQLN
                     VSFLLDSPLFERIALPYQQQLELLGIGVTIKTVDSAQYERQVQTFDYDIIVGSWGQSL
                     SPGNEQRDFWGSEAAKRNGSRNYVGIADPAIDKLIEAIIFAPDRPALITACKALDRAL
                     I"
BASE COUNT          594 a          982 c         1001 g          532 t
ORIGIN      
        1 gcctcgcgtt cggacacggg cagggacacg aaacgctcga ggacggggcg gcgataggcg
       61 tagacgccga tgtggtgcca atggggtggc gggtgatcgg ggccgacctg gcgcaggaag
      121 tcgcgggcaa aggcaacttc ccgttccgcc gacagcggtg cgatcgcctt cacgatgttg
      181 ggattgtcga catccgcctg atcctcgatg cgggccgcga tggtggagat gtcggcctgc
      241 tcgttgacaa gccctgcgag gcagcgctgc accgacagcg gatcgatggt gggcaggtcg
      301 ccctggaggt tgaccacgaa gctgaagcgt ccctccggat cgcgcagctg cagcgcctcg
      361 gcgatccggt cggagcccga gggatgcgcc gggtcggtca cgatggcatc gccgccatgc
      421 tggcgcaccg cctcggcgat ctcgatctcg gccgccgcca ccagcacctg gccgacattg
      481 gcctccacgg cccgcttcca gacgtgcagg atcatcggca ggccatggat atcggccagg
      541 ggttttccgg ggagacgggt agaggccatc cgggccggga tcacgacaat ggtattctgg
      601 ttttcgatca tgggcttaac cgagggcgcg gcaaattggt caagcggtct ttatcgtatt
      661 gcggttgcca agagaaacaa ttagtttccg cgcccgatca ggggcttcag gggttcggat
      721 tcctcatatg gacagttttg aattcaacaa gattgccggc gccgtgctcg gcaccgcgct
      781 catggtgttc ggcctgcacg aactggcagg catcatctat catccgcatg aaccggagaa
      841 gccgggcttc gtggtggcgg tgccggaagg cgaggcgccc gcgggcggcg agaccgcggc
      901 ggccgaaccg gaagccccgg ccgagtcgct cggcgcgctt ctggcggcgg ccgatccggc
      961 caagggccag gccggcttca aagcctgcgc ggcctgtcac gatgtgacca agggcggtcc
     1021 gaacaaggtc gggcccaacc tctggggcgt tgtgggtgcg atgcatggcg tccacgccgg
     1081 cttccagtat tccgatgcca tggccgccaa gtcgggcgaa ccctggacct atgaagcgct
     1141 caacgaattc ctgacggccc cggcaaaggc gatcccgggc accaagatgg cattcggcgg
     1201 cgtcaagaag gcgcaggacc gggccaatct cctggcctac ctggccacgc tgtcggatgc
     1261 gccggtgccc ttccccgccc cgtaaaggcc gcaaagctta tcgaaattta acaccggcaa
     1321 tcatcttgcc ggtgttttct tttgcaccgc cgcttgcgta tgttcgtcat gaaacgatcc
     1381 gtcttcacgc ccggagtgca aagccccatg accctgagcc gccgttcgct gctgaaactc
     1441 gccggccttt cgccgcttgc caagattgcc ggcatccagc tggccagcgc cgaggaccgt
     1501 gacttccgcc atgcgctgac gctgttcagc gacatcaagt acgggccgga cttcaagcac
     1561 tttgactatg tcaacccgga cgcgccgaag atggggcgcg tgcggttcgg catcgtcgga
     1621 tccttcgaca gcctcaatcc ctataccttc aagggcgatc ccgggggtgc cgtggtcaac
     1681 gacgcgctgc tcgtctcttc gctcgacgaa gcctccaccg agtatggtct ggtggcgagc
     1741 gccgtgtggc atcccgagga ccggtcgatg gtgatctatc gcctgcggcc cgaagcgcgc
     1801 ttccatgacg gcaagcccat gacgcccgag gacgtgatct ggagcatgca gtcgctgcgc
     1861 gaggcgcatc ccttctacaa tgcctactac aagaacatca gcaaggccga gcagaccggc
     1921 gaacatgagg tgaccttcat gttcagcgag acgggaaacc gggagctgcc gctgatcacc
     1981 ggccagatgc cagtgctgcc gaagcactgg tggactggca aggatgccaa gggacggcag
     2041 cgcgatctca aggagacgac gttggaagtg ccgctcggtt ccggcgccta tgtggccacc
     2101 gagatcaagc cgggcgcctc gatcaggatg aagcgcgtcg ccgactactg gggccgggat
     2161 ctgcccgtga atgccggcca ggacaatttc gaggagatgg aatacatcta cttccgcgat
     2221 gccaatgtgg ccctcgaggc cttcgccggc gatcagtatg actggcggct ggagaattcc
     2281 tcgaagagct gggccaccgg ctacgacttt ccggcgatca aggacgggcg cgtgatcaag
     2341 gaagagatca cgctgaagca ggtcgagggc atgcagtcct gggccatgaa catccgcagg
     2401 ccgaagttcc aggacgtgcg cgtccgccag gccttgaatt atgcgttcga cttcgaatgg
     2461 gccaacacca atctcttcta cggccagtac aagcgctcgc gcagttactt caacaactcc
     2521 gaaatggaag caaaggggct tccctcgcca gaggagctga agcttctcga gccgctgcgc
     2581 gaccagctgc cgcccgaggt cttcacggct gaattcgcca atccggtgaa cgacacgccg
     2641 cagaaccggc gcaagaacct gcgcgaggcc tcccggctcc tggaccaggc cggatggaag
     2701 gtcacgcagg acggcaacag gtcgcttctc aagaacgaca agggcgaaca gctcaacgtg
     2761 agcttcctgc tcgactcgcc cttgttcgag cgcatcgccc tgccctatca gcagcagctc
     2821 gagctactcg gcatcggcgt caccatcaag accgtggaca gcgcgcaata cgagcgccag
     2881 gtgcagactt tcgactacga catcatcgtc ggcagctggg gccagtcgct gtcgccaggc
     2941 aacgagcagc gcgacttctg gggctcggag gcggcgaagc ggaatggcag ccgcaactat
     3001 gtcggcatcg ccgatccggc catcgacaag ctgatcgagg cgatcatctt cgcgcccgac
     3061 cggccggccc tcatcaccgc ctgcaaggcg ctggaccggg cgctcatct
//
LOCUS       DBNGVR010000394         1576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1324103, whole genome shotgun sequence.
ACCESSION   DBNGVR010000394 DBNGVR010000000
VERSION     DBNGVR010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1324103"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC16A0_08380"
     CDS             complement(<1..246)
                     /locus_tag="AC16A0_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105390.1"
                     /translation="MTRASRPRRGGPLSSGRRLLAIALRSTEVIAAAAFFAVLTVFFL
                     FFAGGLGLPGWTVAVTAIAGSLAVSVLAFSALHDPDHT"
     gene            complement(248..>1576)
                     /locus_tag="AC16A0_08385"
     CDS             complement(248..>1576)
                     /locus_tag="AC16A0_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3105391.1"
                     /translation="CKPTVYTPSGIAMWRPMAEALGWPGKPVSWKSIIALASDPQGWG
                     RYGHPEWGQLKLGYTHPQYSSAGLLFLASVIYGQIGKTEGLTPQDVYSRPVEQALSAM
                     ARNTSKYGMVTTNLLDMMARQGPDFLHAIAGFEEGVVRFNLERQAELRFPLAFVFPSE
                     GTFWSDHPYCILDGAPWVTPERAEAAGLFGRYLRDSEQQAAAGDFLLRPLDATRGSGS
                     HLMLANGTDPAPRPETIKPFEVPGAEAAGAIIDQFLATKRKARVLLVLDVSSSMNGAP
                     IRAATEATARFLKRLDPRDEVALMVFNDGIRLVSDFAPAAGGAEGLSQRVLQLVSGGG
                     TNLNGAVCAATEAMAKARKSASGEGRLNGIVLLSDGADTAGAISETRMLQTCLPGSAE
                     ADGTKILAIAFGDGADREALNRIAHQTGGAVFAADPGTIDQTYLRISAEQ"
BASE COUNT          272 a          515 c          542 g          247 t
ORIGIN      
        1 ggtatgatcg ggatcgtgca gagcggagaa ggccagcacg gagaccgcca gagagccggc
       61 gatcgcggtg acggccacgg tccagccggg aagcccaagt cctccagcga agaagaggaa
      121 gaagaccgtc agcacggcga agaaggccgc cgcggcgatg acctcggtgc tgcgcagggc
      181 gatggcgagc aggcggcgcc cggaggacag gggaccacct cgcctaggcc gtgaagccct
      241 tgtcatgtca ctgctcggcc gagatgcgca gataggtctg gtcgatcgtg ccgggatcgg
      301 cggcgaagac ggcgccgccg gtctgatgcg cgatacggtt cagagcctcg cggtcggcgc
      361 cgtcaccgaa ggcgatggca aggatcttgg tgccatccgc ttcggccgaa cccggcaggc
      421 aggtctgcag catgcgggtt tccgagatgg cacccgcggt gtcggccccg tcggacagca
      481 ggacgatgcc gttcagccgc ccctcgcccg acgccgactt gcgcgccttc gccatggcct
      541 cggtggcggc gcagacagcg ccattcagat tggtgccgcc gcccgacacc agctgaagca
      601 cgcgctgcga cagcccttcg gcaccgcctg cggccggcgc gaaatccgag accagccgga
      661 ttccgtcgtt gaagaccatg agcgccacct cgtcgcgcgg atcgagccgc ttgaggaagc
      721 gtgccgtggc ctcggtggcc gcgcggatcg gcgctccgtt catgctgctt gagacgtcca
      781 acacaagcag cacgcgcgcc ttgcgcttgg tcgcgagaaa ctggtcgatg atggcgccgg
      841 ctgcctcggc tccgggaacc tcgaagggct tgatcgtctc gggccgcggc gcagggtccg
      901 ttccgttggc cagcatgagg tgcgaaccgc tcccgcgcgt cgcgtcgagc ggacgcagca
      961 ggaaatcgcc cgccgccgcc tgttgctcgc tgtcacgcag atagcggccg aacagacctg
     1021 cggcttcggc ccgctccggc gtcacccagg gcgctccatc gagtatgcaa tagggatgat
     1081 cgctccagaa ggtcccttcg gacggaaaga cgaaggcaag cggaaagcgc agctcggcct
     1141 ggcgttccag attgaagcgc accacgcctt cctcgaaccc ggcaatggca tggagaaagt
     1201 ccggtccctg ccgggccatc atgtccagca ggttggtggt taccattcca tactttgacg
     1261 tgttgcgcgc cattgccgaa agggcctgct cgacggggcg cgaatagaca tcctgtggcg
     1321 tgaggccctc ggtctttccg atctgcccgt agatgactga cgccagaaag agcaggccgg
     1381 cgcttgagta ttgcgggtgg gtatagccga gcttcagctg gccccattcg gggtgcccat
     1441 agcggcccca gccctgcgga tccgacgcca gcgcgatgat ggatttccag ctgaccggtt
     1501 ttcccggcca gccgagggcc tccgccatcg gccgccacat cgcgattccg ctgggcgtgt
     1561 agacggtggg cttgca
//
LOCUS       DBNGVR010000395         2847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3681222, whole genome shotgun sequence.
ACCESSION   DBNGVR010000395 DBNGVR010000000
VERSION     DBNGVR010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3681222"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="AC16A0_08390"
     CDS             complement(<1..281)
                     /locus_tag="AC16A0_08390"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3105392.1"
                     /translation="MIPNRNFNYPTAVKFGAGRIKELAELCKANGIHRPLFVTDPGLA
                     AMPMVKEIVADVKKAGLGIEVFSDVRPNPVEANIEAGVKAYKAGRHDGV"
     gene            complement(278..1669)
                     /locus_tag="AC16A0_08395"
     CDS             complement(278..1669)
                     /locus_tag="AC16A0_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3105393.1"
                     /translation="MAETIKCISPVDGSLYASRPVASRKDIAATFAAAHAAQAKWRKV
                     PLKERAAHCLAALEAMLAMKDEIVPELAWQMGRPVRYGAGELRGFEERARYMIAVAER
                     ALEAIVPDPKEGFLRFIRREPLGVVFTIAPWNYPYLTAVNSIIPAIMAGNTVVLKHAA
                     NTLLVAERFQKAFDKAKLPRGVFQHLVLNHKQTADIISGGHANMVCFTGSVAGGKAME
                     QAAAGRFINLGLELGGKDPAYVRPDANLAHAIENLVDGAYFNSGQSCCGIERIYVHKK
                     VWGDFIDGFVDLTRNYVLGSPLDEATTLGPLVKAEAADFVRKQIRAALRAGATAHVDP
                     RSFALDKTGTPYMAPQVLTNVNHQMSVMMEESFGPVVGIMKVADDAEAIELMNDSPYG
                     LTAAIWTEDAAAAQRIGDEIATGTVFMNRCDYLDPALTWTGVKDTGRGATLSVVGYEA
                     LTRPKSYHLRTRT"
     gene            complement(1678..>2847)
                     /locus_tag="AC16A0_08400"
     CDS             complement(1678..>2847)
                     /locus_tag="AC16A0_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR3105394.1"
                     /translation="GYKASSWEKGYGDFVLKPDMATLRRIPWLPATALVLCDILDHHT
                     HQDIPHSPRAILKKQLKRLDALKMKAYMASELEFFLFDDSFEQAHEKGYRNLRTSANY
                     IEDYHIFQTSKEEGVMRAIRNGLHGAGIPVENSKGEAAPGQEEINVKYAEALDMADRH
                     VIIKNGCKEIAHGLGKAITFMAKWNYALAGSSSHIHQSLWSADGKTPLFFDKAGEHGM
                     SALMQNYMAGQLQHSGELTYFLAPYMNSYRRFIAGTFAPTRAVWSFDNRTAGFRLCGG
                     DTKAIRVECRIGGSDLNPYLAFAALLAAGLDGIEKKMKLEPAFSGDAYDTSKKLREIP
                     KTLREATALLDGSKMLRAAFGDEVVDHYVHTAKWEQFEYDRRVMDLDLFRGFEQY"
BASE COUNT          510 a          896 c          885 g          556 t
ORIGIN      
        1 atgacgccgt catgccgccc ggccttgtag gccttgaccc ctgcctcgat gttggcctcc
       61 accggattgg gccgcacatc ggagaaaacc tcgatgccga ggcctgcctt cttcacgtcg
      121 gccacgatct ccttgaccat cggcatggcc gcaagtccag ggtcggtgac gaagaggggg
      181 cgatgaatgc cattggcctt gcacagctcg gccagctcct tgatgcggcc cgcaccgaac
      241 ttgacggccg tcgggtaatt gaaattgcga ttgggaatca tgttctggtc ctcaggtgat
      301 agctcttggg tctcgtcaat gcttcgtatc cgacaaccga cagcgtagcg ccgcggcccg
      361 tatccttcac gcccgtccag gtcagtgccg gatcgagata gtcgcagcgg ttcatgaaga
      421 ccgtgccggt ggcaatctcg tcgccgatcc tctgggccgc agccgcgtcc tccgtccaga
      481 tcgccgccgt caatccatag ggcgaatcgt tcatgagttc gatcgcctcc gcatcgtcgg
      541 ccaccttcat gatgccgaca accgggccga agctctcttc catcatcacg ctcatctggt
      601 ggttgacgtt ggtcagcacc tgcggcgcca tgtagggcgt gccggtcttg tcgagggcga
      661 aggacctggg atcgacatgc gccgtggcac cggcgcgaag ggccgcgcgg atctgcttgc
      721 gcacgaagtc ggcagcctcg gctttcacca gcggcccgag agtcgtggcc tcatcgagcg
      781 gcgaacccag cacgtagttc cgggtgaggt ccacgaagcc gtcgatgaag tcgccccaga
      841 ccttcttgtg cacgtagatg cgctcgatgc cacagcagct ctgcccggaa ttgaaatagg
      901 ccccatcgac gagattctcg atggcatggg caagattggc gtcaggccgc acataggccg
      961 gatccttgcc gccaagttcg aggccaagat tgatgaagcg cccggccgcg gcctgctcca
     1021 tcgccttgcc gcccgcgacc gaaccggtga agcagaccat gttggcatga ccgccggaaa
     1081 tgatgtccgc ggtctgcttg tggttcagca ccaggtgctg gaacacgcct ctgggcagct
     1141 tcgccttgtc aaaggccttc tggaaccgct cagccaccag cagggtattg gcggcgtgct
     1201 tcagcaccac ggtattaccc gccatgatcg ccgggatgat cgagttgacg gccgtcagat
     1261 agggatagtt ccagggcgcg atggtgaaga ccacgcccag cggctcgcgc ctgataaagc
     1321 gcaggaagcc ctccttggga tccggcacga tggcctccaa cgcccgttcg gcgacggcga
     1381 tcatgtagcg ggcgcgttcc tcgaagcccc tgagttcgcc cgcgccataa cggacgggac
     1441 gtcccatctg ccaggcgagt tcgggcacga tctcgtcctt catcgccagc atcgcctcga
     1501 gggcggcgag gcaatgagcg gcacgctcct tcagcggcac cttccgccac ttggcctggg
     1561 ccgcatgagc ggcagcgaag gtggctgcga tgtccttgcg gctggcgacc ggccggctgg
     1621 catagaggct gccgtcaacc ggcgagatgc atttgatggt ttcggccatg gcgttcctca
     1681 gtactgctcg aagccgcgaa agaggtccag atccatcacg cggcgatcat attcgaactg
     1741 ctcccacttg gcggtatgca catagtggtc gacgacctcg tcaccgaagg cggcgcgcag
     1801 catcttcgat ccatcaagca gggcggtcgc ctcgcgcagc gtcttgggga tctcgcgcag
     1861 cttcttcgac gtgtcatagg catcgcccga gaaggcgggc tcaagcttca tcttcttctc
     1921 aatgccgtcg agacctgccg caagcagcgc agcgaaggca agatagggat tgagatcgga
     1981 gccgccgatg cggcactcca cgcggatagc cttggtatcg ccgccgcaaa ggcggaagcc
     2041 tgccgtgcga ttgtcgaagc tccagaccgc gcgcgtcggc gcgaaggtgc cggcgatgaa
     2101 gcggcggtag gagttcatgt agggcgcaag gaaataggtg agttcgcccg aatgctgaag
     2161 ctggcccgcc atgtagttct gcatcagcgc cgacatgccg tgctcgcccg ccttgtcgaa
     2221 gaacagcggc gtcttgccat cggcgctcca caacgactga tgaatgtgcg acgaactgcc
     2281 cgccagcgcg taattccact tggccatgaa ggtgatcgcc ttgcccagcc catgggcgat
     2341 ctccttgcag ccgttcttga tgatcacgtg ccggtcggcc atgtcgaggg cctcggcata
     2401 cttcacgttg atctcttcct ggccgggcgc cgcctcgccc ttggagttct cgaccggaat
     2461 acccgcgcca tgcagcccgt tgcggatcgc ccgcatcacg ccttcttcct tggaggtctg
     2521 gaagatgtga tagtcctcga tatagttggc cgaggtcctg agattgcgat agcccttctc
     2581 atgggcctgc tcgaagctgt cgtcgaagag gaagaactcg agctcggaag ccatgtaggc
     2641 tttcatcttc agcgcatcca ggcgcttcag ttgtttcttg aggatcgcgc gcggactgtg
     2701 cgggatgtcc tgatgcgtgt gatgatcgag gatgtcgcag agcaccagcg ccgtggcggg
     2761 cagccaggga atgcgccgca gcgtcgccat gtcgggcttg agcacgaaat cgccatagcc
     2821 cttttcccag ctcgaggcct tgtagcc
//
LOCUS       DBNGVR010000396         2320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3681271, whole genome shotgun sequence.
ACCESSION   DBNGVR010000396 DBNGVR010000000
VERSION     DBNGVR010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3681271"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="AC16A0_08405"
     CDS             <1..147
                     /locus_tag="AC16A0_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3105395.1"
                     /translation="TRLSESGFSDAEIARIRGPIGLSIGAKGAPEIAVSIMAEMTRAL
                     RLGE"
     gene            149..1765
                     /locus_tag="AC16A0_08410"
     CDS             149..1765
                     /locus_tag="AC16A0_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR3105396.1"
                     /translation="MKFGELPVAEAEGAILAHSVRHGSGTFKKGRVLSLADISALRSA
                     GVDRVFAARLAADDVPEDKAAAAVAQVIAGEATRAQAPFTGRANLHATERGIAIVDTA
                     RVSALNRLHESLTLATVAPYAVVDRREMVATVKVIPFAVPASVLQEALRIAGSEPIVR
                     IEAFSSKRAGLVITRLPQTKPSLIAKSEDAMRERVAALDGTITQVLVVDHAIDKVSAA
                     IRELMGNGVDPVLVFGASAIVDRGDVVPAALVSAGGEVLHLGMPVDPGHLMMLGRLGD
                     VPVIGVPSCARSPKLNGFDWVLARVMADVQVTAEDIMEMGAGGLLAEIPSRPSPREGR
                     PKVQRAPRVTAIVLAAGTSSRMGSNKLLADIGGKPMVRHTVAALQQASIDQIVVVTGR
                     DSDHVIAALQSLPVICVHNPDFAHGLSTSLRRALEVVPEDSDAVLVCLGDMPLVDEAV
                     ANRLIAAFSPAEHRSICVPVHQGMRGNPVLWGKAHLPALMDVTGDRGGKTLLELRADE
                     VVEVEMPDRAVLTDIDTPQALDDIRSGRVP"
     gene            complement(1743..>2320)
                     /gene="radC"
                     /locus_tag="AC16A0_08415"
     CDS             complement(1743..>2320)
                     /gene="radC"
                     /locus_tag="AC16A0_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3105397.1"
                     /translation="LFRVIRRGDTKPLAKALLAHFGSFAEVLSASPERLMEVRGVGEA
                     IAVELKIVHAAALRLLRGQVIDRPALASWSAVIDYIRAAMAFSRIEQFRILFLDKKNQ
                     LIADEVQQQGTVDHTPVYVREVMKRALDIGASAIVLVHNHPSGDPTPSPADIEMTRRI
                     VDAGQKLQIAVHDHIIVGRKGHASFRALGLI"
BASE COUNT          402 a          714 c          777 g          427 t
ORIGIN      
        1 acccggcttt cggaatctgg attttcggat gcagagatag cccgcattcg cggtcccatc
       61 gggctcagca tcggtgccaa gggggcgcct gaaatcgctg tgtccatcat ggcggagatg
      121 acgcgggcgc tgcgcctcgg agagtgagat gaaattcgga gaactgccgg ttgcggaggc
      181 cgaaggcgcc attctcgccc attcggtcag gcatggcagc ggtacattca agaaggggcg
      241 ggtgctttcg ctggctgaca tctctgctct tcgatctgcc ggcgtcgaca gggtttttgc
      301 tgcacggctt gcagcggatg atgtgccgga ggacaaggct gccgcagccg tggcgcaggt
      361 catcgcaggc gaggcgacgc gagcccaggc gccgttcacg ggacgcgcca atctccatgc
      421 caccgagaga ggcatcgcca tcgtcgatac ggccagagtt tccgcactca accgcctgca
      481 cgagagcctg accctggcca ccgtcgcgcc atacgccgtc gtcgaccggc gtgagatggt
      541 ggcgaccgtc aaggtcattc ccttcgccgt tccggcatcg gtgttacagg aggccctccg
      601 gattgcggga tccgagccca tcgtccgtat tgaagctttc agcagcaagc gggccggact
      661 tgtcatcacg cgcttacccc agaccaagcc atcgctgatc gcaaagtcgg aagatgccat
      721 gcgcgagcgc gtcgcggcgc tcgacggcac aatcacgcag gttctcgtag tggaccatgc
      781 catcgacaag gtgagcgcag caatccgcga actcatgggc aacggtgtcg atcccgtcct
      841 ggtcttcggt gcatctgcaa tcgtcgatcg cggcgatgtc gtgccggccg cactggtttc
      901 cgcgggcggc gaagttctgc accttggcat gccagtggat ccgggccacc tcatgatgct
      961 gggccggctg ggtgacgtgc cagtcattgg cgtgccctcc tgtgcgcgct cgcccaagct
     1021 caatggtttc gattgggtcc ttgcccgcgt gatggccgat gtccaggtga cggcggagga
     1081 catcatggaa atgggggcag gcgggttgct ggccgaaatc ccgtcgcgtc catcgccgcg
     1141 cgaggggcgg cccaaggtgc agcgtgcccc gcgggtgacc gccatcgtgc tcgcggcggg
     1201 cacctcctca cgcatgggca gcaacaagct gctggctgac atcggcggca agccgatggt
     1261 caggcatacg gtggcggcct tgcaacaggc ctcgatcgat cagatcgtgg tggtgaccgg
     1321 gcgcgattcc gatcacgtga tcgcggcgct gcagagcctt ccggtgatct gcgtgcataa
     1381 tccggacttc gcacatggcc tgtcgacatc gctgcgccgg gcgctggaag tggtgccgga
     1441 agacagcgat gccgttcttg tctgccttgg ggatatgcca cttgtcgatg aggcggtggc
     1501 aaaccggctg atcgcggcct tcagtcccgc cgaacaccgc tccatttgcg tacccgtgca
     1561 tcagggaatg cgcggcaatc cggttctgtg gggcaaggca cacctgccgg cactcatgga
     1621 cgtgacgggc gaccgcggtg gaaagaccct gctcgagctg cgcgccgacg aggttgtaga
     1681 ggtcgagatg cccgaccggg ccgtgcttac ggacatcgac acgccgcagg cgctcgatga
     1741 catcagatca ggccgagtgc cctgaagctc gcatggcctt tgcggccgac gatgatatga
     1801 tcgtgcaccg cgatctgcag cttctggccc gcgtcgacaa ttctgcgcgt catttcgatg
     1861 tctgcaggcg acggcgtggg gtcgcccgaa gggtgattgt gaaccaggac gatggccgag
     1921 gcgccgatgt ccagcgcgcg cttcatcacc tcccgaacat agacgggcgt gtgatcgact
     1981 gtgccctgct gctgcacctc gtccgcgatc agctggttct tcttgtcgag gaagagaatg
     2041 cggaactgct cgatacggga gaaggccatg gcggcccgga tgtagtcgat caccgcgctc
     2101 cagctggcga gagcagggcg gtcgatgact tgcccgcgta gcaggcggag cgcggcggca
     2161 tggacgatct tcagttccac ggcaatggct tcgcccacgc cccgcacctc catcaggcgc
     2221 tccgggcttg cgctcaggac ttcggcaaag ctgccaaagt gtgcgagaag ggctttcgcc
     2281 aggggcttcg tatcgccacg ccggatgacg cggaacagaa
//
LOCUS       DBNGVR010000397         5724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_538127, whole genome shotgun sequence.
ACCESSION   DBNGVR010000397 DBNGVR010000000
VERSION     DBNGVR010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_538127"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="AC16A0_08420"
     CDS             <1..1960
                     /locus_tag="AC16A0_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HIR3105398.1"
                     /translation="ITNFGGGNTSAKVKMKDPLTGGDVTVLWVKGSGGDIGSMKLDGF
                     ATLDMDKLESLKGLYKSLDQEDAMVHALNHCIYNLNSRAPSIDTCLHGYVPYAHVDHV
                     HSDAVIAIAASKDSERLTKEIFGSEMGYLPWQRPGIDLGIKLGDMAKAHPEYVGVVLG
                     SHGLFTWANDARTCYDTTLRIINKAAVWLERNGKKPAFKGEKVKSLPEAERRKVARRL
                     LPEIRGRISKHEMKVGHFVDSSEVMEFVNSKQLDQLAPLGTSCPDHFLRTKIRPLIVP
                     HVAEGAALDALIESYRKDYAAYYEACKHADSPAMRDPNAVIYLVPQVGMLSFAKDKAT
                     ARIAAEFYVNAINVMRGASSVSEYVGLPAQEAFNIEYWLLEEAKLQRMPKPKSLAGRI
                     ALVTGGAGGIGSAIASKLLSEGACVVLADIDKASLDEVTAGFGKTYGKDNVRGVVMDV
                     TSEDAVIGATDQAVLAYGGLDIVVNNAGITLAAPVEDTSLELWNKNMAILGTGYFLVA
                     REGYRLMQKQKLGGSMIFIGSKNGVTASPGASAYCSVKAAEIQLARCMALEGAPHGIR
                     VNVVNPDAVLRGSKIWQGPWRQQRAASLKISEDELEEHYRKRSLLQRSVYPEDIAEGV
                     YFFASDLSNKSTGNFLNIDAGNAASFTR"
     gene            1965..3257
                     /gene="rhaI"
                     /locus_tag="AC16A0_08425"
     CDS             1965..3257
                     /gene="rhaI"
                     /locus_tag="AC16A0_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HIR3105399.1"
                     /translation="MGFAISADLIGTANGKLEAALADDYEALGRQLERRGIAIEEMTT
                     RAMDFAVAVPTWGVGTGGTRFARFPGPGEPRNIHDKLEDCGVIQQLARATPNVSPHFP
                     WDKVSDYNALRQEAASHNLGFDAVNSNTFQDQKAQRHSYKFGSLTHADRATRQQAVEH
                     NLECIEIGQKLGSTALTVWIADGSNFPGQANLDAAFDRYIESLRTIYDALPKNWLVFL
                     EHKLYEPAFYSTVISDWGSNLMAAQELGPKARCLVDLGHHAPNTNNEQIVARLIRAKK
                     LAGFHFNDSKYGDDDLDSGSVDPFRLFLVFNELVDAELRKAEGFAPAYMIDQSHNVTD
                     PIESLIASAVEIQRAFVQASLIDRKALAAAQDASDVMLGHRLVKQAFVTDVSPILAEA
                     RRRKGGAIDPIATYRTSGYRARKAKERPAVEGTSAGIV"
     gene            3304..4401
                     /locus_tag="AC16A0_08430"
     CDS             3304..4401
                     /locus_tag="AC16A0_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HIR3105400.1"
                     /translation="MDIRDYLDYEPAGRRIEDLDTPVPLIDIGIAERNLKRWQARTDA
                     LGLGNRPHIKTHKLAGLARYQRDLGARGITVQKLGEAEVMADAGLSDMLVTFNIVGRS
                     KLERLAALARRTEIMVVADNAAVVEGLGWAGTSAGRDLRVLVECDTGAHRNGVQSPEA
                     AIALARTIDATPGLAYGGLMTYAKPGTRRGAADYLSAARGGASRAGLETKVISSGGSP
                     DMWKDEGLDVLTEYRVGTYIYFDRSLAERQVCSYDDCAVSILATVVSVPTPERALLDA
                     GSKSLTSDLLGMRGYGTVRSLDGATVYDLSEEHGFLDISGSANKPKVGDRLRIVPNHV
                     CPVINLFDRVVVVDGEEVLGAVRVDARGLVQ"
     gene            complement(4403..4798)
                     /locus_tag="AC16A0_08435"
     CDS             complement(4403..4798)
                     /locus_tag="AC16A0_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3105401.1"
                     /translation="MAETQVDTKVAGAEESFLFIGELAKQTGIDPKTIRFYERAELLS
                     PPRHGKFRTYLASDVRRLQNILRLRRLGVPIAQIRDVLERVGDCPNILEIEQAVSMLR
                     AHLDVLRSKHVEISRQIEQTATLVETRAA"
     gene            complement(4973..5527)
                     /gene="hpt"
                     /locus_tag="AC16A0_08440"
     CDS             complement(4973..5527)
                     /gene="hpt"
                     /locus_tag="AC16A0_08440"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR3105402.1"
                     /translation="MSEQIIEGHRIGVLFSESQIADRITELAREIAARKPERLLVVPV
                     LKGSFIFAADLIRAMHHAGLSPEVDFMMLSSYRAATRSSGQVQVLRDIESDVKGRHVL
                     LVDDILESGRTLAFAKDLISARGAARVYTAVLLNKPGHLAANIGADFECFKCPDKFVV
                     GFGMDMAHQFRELPFVGHVLGPAG"
     gene            complement(5524..>5724)
                     /locus_tag="AC16A0_08445"
     CDS             complement(5524..>5724)
                     /locus_tag="AC16A0_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105403.1"
                     /translation="IPWIRFALRSDSNDEVYNWTLDAGARPLKSGEITNFVTRVASPP
                     ETAKNLEIRFAHAHEIGSNAAP"
BASE COUNT         1070 a         1880 c         1810 g          964 t
ORIGIN      
        1 catcaccaat ttcggcggcg gcaatacctc ggcaaaggtg aagatgaagg atccgctgac
       61 cggcggcgac gtcaccgtgc tgtgggtcaa gggctccggc ggcgacattg gctcgatgaa
      121 gctcgacggc ttcgccacgc tcgacatgga caagctcgaa tccctgaagg ggctctacaa
      181 gagcctcgat caggaagacg cgatggtgca cgcgctgaac cactgcatct acaatctcaa
      241 ttcccgtgcg ccgtcgatcg acacctgcct gcatggctat gtgccatatg cccacgtcga
      301 tcacgtccat tccgacgcgg tcatcgccat tgccgcctcg aaggactcgg agcgcctgac
      361 caaggagatc ttcggcagcg agatgggcta cctgccctgg cagcgccccg gcatcgacct
      421 cggcatcaag cttggcgaca tggccaaggc gcatcccgaa tatgtcggcg tggtgctggg
      481 cagccacggc ctcttcacct gggccaatga cgccaggacc tgctacgaca cgacgctgcg
      541 catcatcaac aaggccgccg tctggcttga gcgcaacggc aagaagcccg ccttcaaggg
      601 cgagaaggtc aagagcttgc cggaggcgga gcggcgcaag gtggcgcgcc gcctgttgcc
      661 cgagattcgc ggccgcatct cgaaacacga aatgaaggtc ggacacttcg tcgattcgag
      721 cgaggtgatg gaattcgtga attcgaagca gctggaccag ctggcgccgc tcggcacctc
      781 gtgccccgat cacttcctgc gcaccaagat ccgcccgctc atcgtgccgc atgtcgcgga
      841 gggcgcggcc ctcgacgcat tgatcgagag ctaccgcaag gactatgcgg cctactacga
      901 ggcctgcaaa catgccgact cgcccgccat gcgcgatccc aatgcggtga tctatctcgt
      961 gccgcaggtc ggcatgctgt ccttcgccaa ggacaaggcc acggcgcgca tcgccgccga
     1021 attctacgtc aatgccatca acgtcatgcg cggcgcatcc agcgtgtccg aatatgtcgg
     1081 gctgccggcc caggaggcct tcaacatcga atactggctt ctcgaggaag ccaagctgca
     1141 gcgcatgccc aagcccaaga gcctcgcggg ccgcatcgcc ctggtgaccg gcggcgccgg
     1201 cggcatcggc tcggccatcg cttcaaagct cctgtcggaa ggcgcctgcg tggtgctggc
     1261 cgatatcgac aaggcctcgc tcgacgaggt cacggcgggc ttcggcaaga cctatggcaa
     1321 ggacaatgtc cgcggcgtgg tcatggacgt caccagcgag gacgccgtca ttggcgcaac
     1381 cgaccaggcc gtgctggcct atggcggcct cgacatcgtg gtgaacaatg ccggcatcac
     1441 cctggcggca ccggtggagg acaccagcct cgaactgtgg aacaagaaca tggcgatcct
     1501 cggcaccggc tatttcctgg tggcgcgcga gggctatcgc ctgatgcaga agcagaaact
     1561 gggcggctcg atgatcttca tcggctccaa gaacggcgtc actgcctcgc ccggcgcctc
     1621 ggcctattgt tcggtgaagg ccgccgagat ccagctggcg cgctgcatgg cgcttgaggg
     1681 cgcgccgcac ggcatccgcg tcaatgtggt gaacccggat gcggtgctgc ggggctccaa
     1741 gatctggcag gggccgtggc gccagcagcg cgccgcctcg ctcaagatca gcgaggacga
     1801 actcgaggaa cattaccgca agcggtcgct cctgcagcgc tcggtctatc cggaagacat
     1861 cgccgagggt gtctacttct tcgcctccga tctctccaac aagtcgaccg gcaacttcct
     1921 caacatcgac gcgggcaatg ccgcgtcctt cacgcgctag tccgatgggc ttcgccatct
     1981 cggccgatct catcggcacc gccaacggca agctggaggc tgcactcgcc gacgactatg
     2041 aagcactcgg ccgccagctc gaacggcgcg gcatcgccat cgaggagatg acgacacgtg
     2101 ccatggattt cgccgtggcg gttcccacct ggggcgtggg caccggcggc acgcgcttcg
     2161 cccgcttccc cggtcctggg gaaccgcgca acatccatga caagctcgag gattgcggcg
     2221 tcatccagca gctggcgagg gccacgccca atgtctcgcc gcattttccc tgggacaagg
     2281 tctccgacta caacgcgctc cgccaggaag ccgcctcgca caacctggga ttcgacgcgg
     2341 tgaattccaa caccttccag gaccagaagg cacagcggca ctcctacaag ttcggttcgc
     2401 tgacccatgc cgacagggcc acgcggcagc aggctgtcga gcacaatctc gaatgcatcg
     2461 agatcgggca gaagctcggc tccacggcgc tcaccgtatg gattgccgac ggctcgaact
     2521 tccccggcca ggccaatctc gacgccgcct tcgaccgcta catcgagtcg ctcaggacca
     2581 tctacgacgc gctgccgaag aactggctcg tcttccttga gcacaaactc tacgagcccg
     2641 ccttctattc caccgtgatc tccgactggg gctccaacct gatggcggcg caggagctcg
     2701 gccccaaggc acgctgcctc gtcgatctcg gccatcacgc gcccaacacc aacaacgaac
     2761 agatcgtcgc gcgtctcatc cgggcgaaga agctggcggg cttccacttc aacgactcga
     2821 agtacggcga cgacgatctc gattcgggct cggtcgatcc cttccgcctc ttcctcgtct
     2881 tcaacgagct ggtggatgcg gaactgcgca aggccgaggg cttcgccccg gcctacatga
     2941 tcgaccagtc gcacaatgtc accgatccca tcgaaagcct gatcgcctcg gccgtcgaaa
     3001 tccagcgcgc cttcgtacag gcctcgctga tcgaccgcaa ggcactggcc gcggcgcagg
     3061 acgccagcga cgtgatgctc ggccaccgcc tcgtcaagca ggccttcgtc accgatgtct
     3121 cgccgatcct ggccgaggcg cggcggcgca agggcggcgc catcgatccc atcgccacct
     3181 accgcaccag cggctaccgc gcccggaagg cgaaggaacg cccggctgtg gagggcacct
     3241 cggccggcat cgtgtgacat tggccgcgcc ctgacatagt ctgtcgcggc aaggagtgcc
     3301 gcgatggata tcagggacta tctcgactac gaaccggcgg gcagacggat cgaggatctc
     3361 gacacgccgg tgccgctgat cgacatcggc atcgccgagc gcaacctgaa gcgctggcag
     3421 gcgcgcaccg acgcgctggg gctcggcaac cgtccgcaca tcaagacgca caagctggca
     3481 gggctcgccc gctaccagcg cgatctgggt gcccgcggca tcacggtgca gaaactgggc
     3541 gaggccgagg tcatggcgga tgcgggactg tccgacatgc tggtgacctt caacatcgtc
     3601 ggccgctcca agctcgaacg ccttgccgcc ttggcgcgcc ggacggagat tatggtcgtc
     3661 gcagataacg cggcggtggt cgaaggtctc ggctgggccg gcacctccgc gggccgcgat
     3721 cttcgcgtgc tggtcgaatg cgacacgggc gcgcatcgca acggcgtgca atcacccgag
     3781 gcagcgatcg cgctggcccg gaccatcgat gcgacaccgg gcctcgccta tggcgggctc
     3841 atgacctatg ccaagcccgg tacgcggcgg ggagctgcgg actacctctc ggcggcgcgc
     3901 ggcggcgcgt cgcgggcggg gctggagaca aaggtaatca gctcaggcgg atcgcccgac
     3961 atgtggaagg acgaggggct cgacgttctc accgagtatc gcgtcgggac ctatatctat
     4021 ttcgaccgct cgctcgccga gcgccaggtc tgcagctacg atgactgcgc tgtctcgatc
     4081 ctcgccacgg tggtgagcgt tcccacgccg gagcgcgcct tgcttgatgc cgggtccaag
     4141 tcgctcacca gcgatctgct cggcatgagg ggctatggca cggtgcgcag cctcgacggc
     4201 gccaccgtct acgacctgag cgaagaacac ggtttcctcg acatctccgg cagcgcgaac
     4261 aagccgaagg tcggcgaccg gctgcgcatt gtgcccaatc acgtgtgtcc cgtgatcaac
     4321 ctcttcgacc gcgtcgtcgt tgtggacggc gaagaggtgc tgggcgccgt gcgcgtcgac
     4381 gcccgcggtc tggttcaata ggtcaggcgg cgcgcgtctc gaccagggtt gcggtctgct
     4441 cgatctgccg cgagatctcg acatgcttgg agcgcagcac gtcaagatgg gcgcgcaaca
     4501 tgctgacggc ctgttcgatc tcgagaatgt tcggacagtc gccgacgcgc tccaggacat
     4561 cgcggatctg tgcgatgggc acgccgagac gccgcaggcg caggatgttc tgcaggcgcc
     4621 ggacgtcgga agcaagatag gtgcggaact tgccatggcg cggcggcgac aggagttccg
     4681 cccgctcgta gaagcggatc gtcttggggt cgatcccggt ctgcttcgcc agttcaccga
     4741 tgaacaggaa agactcttcc gcacccgcaa cttttgtatc gacctgcgtt tccgccatgc
     4801 tccacctccc ttgatcgacc tttgcttccg gcacttcacc gcgccgtgca gtcaaactag
     4861 gaagatcgcc ttagacgtgc aagatagaaa tcgatctcgt ataaattgta attggtttca
     4921 cggacagtgg ccttgcagac agcgaggtgc aaacgatcag gagatgcacg agtcagccgg
     4981 ccggacccag cacatggccg acgaagggca gttcacggaa ctggtgcgcc atgtccatgc
     5041 cgaagcccac cacgaacttg tcggggcact tgaagcactc gaagtcggcg ccgatgttgg
     5101 cggcaaggtg tccgggcttg ttgagcagca cggcggtata gacccgcgcc gcgccgcgcg
     5161 ccgagatcag gtccttggcg aaggccaggg tgcggccgga ctccaggatg tcatcgacca
     5221 gcagcacatg ccggcccttc acgtcggact cgatgtcacg caggacctgg acctggcccg
     5281 aagagcgcgt cgccgcgcgg tagctcgaca gcatcatgaa gtcgacctcg ggggaaagcc
     5341 cggcatggtg catggcgcgg atcaggtctg ccgcgaagat gaagctgccc ttcagcaccg
     5401 gcacgacgag caggcgttcg ggcttgcgcg ccgcgatttc gcgggcgagt tcggtgattc
     5461 ggtcagcgat ctgcgattcg gagaacagca ctccgatgcg gtggccttcg atgatctgtt
     5521 cgctcatggc gcggcatttg agccgatttc atgggcatgg gcaaagcgaa tttcgaggtt
     5581 cttcgcagtt tcgggcggcg atgcgacgcg ggtcacgaag ttggtgatct cgcccgattt
     5641 caacggccgc gcaccggcat cgagcgtcca gttgtagacc tcgtcattgc tgtccgagcg
     5701 cagcgcgaag cggatccagg ggat
//
LOCUS       DBNGVR010000398         4761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3290039, whole genome shotgun sequence.
ACCESSION   DBNGVR010000398 DBNGVR010000000
VERSION     DBNGVR010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3290039"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="AC16A0_08450"
     CDS             complement(<1..734)
                     /locus_tag="AC16A0_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3105404.1"
                     /translation="MASKPRRNLAKSNAQYQRAVKRLPLGVSSTFRYWGDDRTIYVDH
                     GKGGRTWDIDGNCYVDYRLGYGPAILGYADDRVDAAARRGMEVGGVFALSTERELIVA
                     DRIAKMVPAAELVRFSNSGTEAVMAALRLARAHTGRDSYLLVEGGYHGLFDAAMWMAN
                     MDEWDHRSNRDPEIVSYGKGIPRTLRDLAHLVPMNDCERLEDIFKQYGNTLAAMLIEP
                     IQGNCCAISADPEYVKLARELCTRYGV"
     gene            983..2905
                     /locus_tag="AC16A0_08455"
     CDS             983..2905
                     /locus_tag="AC16A0_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR3105405.1"
                     /translation="MTASSHNTEASDDPAVQGHGHAPPSFWILTLGSIGVVYGDIGTS
                     PLYAMREAVLAAMRRDGAINEPMIYGVLSLILWALILIVTLKYVVILLNADNKGEGGT
                     LSLMALAQRAMGTNLVFIPVLGMIGAALFYGDAIITPAISVLSAVEGLKIVTPAFEPY
                     VLWITVAIIVGLFAVQSHGTASVAKFFGPITLVWFIAMGLAGLIHIGDHPGIFHAFNP
                     AYAISFLASHGVIGLVTLGAVFLAVTGAEALYADLGHFGKRPIQVAWIAIVLPCLALN
                     YLGQGAFLLANPAAIESPFFLMVPDWALLPMVVLATAATIIASQVVITGAYSLTRQAI
                     QLGLLPRMEIRFTSEAHAGQIYMPQINWLLLLGVLILVLLFQTSSSLASAYGIAVTGT
                     MVVTATMAIFVIWKYWKWSLASALALMLPLLALDVIFLGANALKIHEGGWVPLAIGAA
                     LMLVMWTWRRGTRILFEKTRRSEVPLKGLADSLAKRPPHRVAGTAVFFTSDPESAPTA
                     LLHSLKHYRVLHEENVVLTIRTSDLPTVPDSEKVKYMPLNDLFSSLIVTFGYMETPHV
                     PEALALARKAGWKFDIMTTSFFLSRRNVRASRHEGMPLWQDQLFIAMARNADDASHYF
                     RLPTDRVVEVGSQITV"
     gene            complement(2910..4106)
                     /locus_tag="AC16A0_08460"
     CDS             complement(2910..4106)
                     /locus_tag="AC16A0_08460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3105406.1"
                     /translation="MLRHMILLVVCVFATPALAQDKGPIQVASGVSYEFLARWDVKKL
                     NQILQTDTPAFTGVKIAYSPAQSAVNLYRVTYNSVIPERGNLPTTASGLLAVPEGAAN
                     GLPMLSYQHGTVYGKQQVPSFPDQSPETQLAIAQFAGQGYVVIGADYFGMGTSDDDEG
                     YVVKASHQQACFDMLVASRVVLDHLNLTAGDLFLGGWSQGGFVTMAFLERLEQAHVEV
                     KAAATASAPVDVFVALNGFLSFPRPIDASWVTTLFILSAFSFEHYYGVPGLARSLIND
                     EYYEVSRKAYEREPFDPADIPTDLHKLIRAEYFDPQFFADSAYGRLVAATQAYRWVIA
                     TPVRNYYGETDEVISIGLGQLAMTYQTAIGNQQVEAISTGKTSHRGTFGTAVPQWKDW
                     FDALNR"
     gene            complement(4214..>4761)
                     /locus_tag="AC16A0_08465"
     CDS             complement(4214..>4761)
                     /locus_tag="AC16A0_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3105407.1"
                     /translation="CGALNLSKPILIGHNMGGIVAFDIAARYPDLPGAIAMLDSAIVL
                     PEATRAAIPAFLERLRGPDYVATLRAYVKERLLLPTDDAVRSAAILEGMGEATQFVAA
                     ASFEGLGDYDSSAARGRVKVPALYIAADEPVPRCDMEGLFDLLPGLRYGQTVGSGHFC
                     QLEVPDQVNAMIDRFVTLARL"
BASE COUNT          869 a         1478 c         1523 g          891 t
ORIGIN      
        1 accccatatc tggtgcagag ctcgcgggcg agtttcacgt attccggatc ggccgagatg
       61 gcgcagcagt tgccctggat gggctcgatc agcatggcgg caagcgtgtt gccgtactgc
      121 ttgaagatgt cttcgaggcg ctcgcagtcg ttcatcggta caagatgggc gagatccctc
      181 agcgtgcgcg ggatgccctt gccataggat acgatctcgg gatcgcggtt ggagcggtga
      241 tcccactcgt ccatgttggc catccacatg gcagcgtcga agaggccgtg atagccgcct
      301 tcgaccagca ggtagctgtc gcggcccgtg tgcgcgcggg ccagccgcag cgccgccatg
      361 acggcttcgg tgccggaatt ggagaagcgc acgagttcgg cggcgggcac catcttcgca
      421 atgcggtcgg cgacgatcaa ctcgcgttcg gtcgacaacg cgaagacgcc gcccacctcc
      481 atgccgcgcc gtgcagcggc atcgacgcga tcatccgcat agccaaggat cgccgggcca
      541 tagccgaggc gatagtcgac gtagcagttg ccgtcgatgt cccaggtgcg tccgcccttg
      601 ccgtgatcga cgtagatggt gcggtcgtcg ccccagtagc ggaaggtgga agagacgccg
      661 agcggcaggc gcttcaccgc ccgttgatac tgggcgttcg acttggcgag attgcgccgg
      721 ggcttgctgg ccatgacagg accttgattg ttccacctgt ccacggattc accagatttt
      781 gactgaattg tcaatcaata tggaatcttc tcgttgcatg aatgcgttac ggcccacagg
      841 ccggcgatca cgagacaggt cacgttgact tacttttcaa atccgacaat gctgcaccgc
      901 aaaatccgcg catgcggatg gcccgaatcc ggcgcatacc gccgcccctg ttgcgatgcg
      961 acagatgcaa aggagaagcg acatgacggc atcaagccac aatacggagg cctccgacga
     1021 tcccgcagtt cagggacacg gccatgcgcc gccaagtttc tggattctga cactgggctc
     1081 gatcggtgtc gtctatggcg atatcgggac cagcccactc tatgcgatgc gcgaagccgt
     1141 actcgccgcc atgcgccggg acggcgccat caacgagccg atgatctacg gcgtattgtc
     1201 gctcattctc tgggcgctca tcctcatcgt cacgctcaag tatgtcgtga tcctgctcaa
     1261 cgccgacaac aagggcgaag gcggcacctt gtccttgatg gccctggcgc agcgcgccat
     1321 gggaaccaac ctcgtcttca ttcccgtgct gggcatgatc ggtgcagccc tcttctacgg
     1381 cgatgccatc atcacgcccg ccatctcggt gctctccgcg gtggaaggcc tcaagatcgt
     1441 gacgccggct ttcgagccct acgtgctgtg gatcacggtg gcgatcatcg tggggctttt
     1501 cgccgtccaa tcgcatggaa cggccagtgt ggcgaagttc ttcggaccga tcacgctggt
     1561 gtggttcatt gccatggggc tggcgggact gatccacatc ggcgatcacc caggcatctt
     1621 ccacgccttc aatccggctt atgccatctc gttcctggcc agccacggcg tcatcggact
     1681 ggtgacactt ggtgccgtct tcctggcggt gacaggcgcg gaagcgctct atgccgacct
     1741 cggacatttc ggaaagcggc cgatccaggt ggcctggatc gccatcgtgc tgccctgcct
     1801 tgcgttgaac tacctcggac agggcgcctt cctgctggcc aatcccgcgg cgatcgaaag
     1861 ccccttcttc ctgatggtac cggactgggc cttgctgccc atggtggtgc tggccaccgc
     1921 ggcaaccatc atcgccagtc aggtcgtgat caccggcgcc tattcgctca cgcgccaggc
     1981 gatccagctg ggcctgctgc cgcgcatgga gatccgcttt acctcggaag cccatgccgg
     2041 gcagatctac atgccgcaga tcaactggct gctgctcctt ggcgtgctca tcctggtgct
     2101 gctgttccag acgtcgtcga gcctcgcttc ggcctatggc atcgcggtga ccggcacgat
     2161 ggtggtgacc gcgaccatgg cgatcttcgt gatctggaaa tactggaagt ggtcgctcgc
     2221 ctctgcgctg gcgctcatgc tgccactgct ggcgctggat gtgatcttcc tgggcgccaa
     2281 tgcgctgaag atccacgagg gcggctgggt gccgctggcc atcggcgcag ccctcatgct
     2341 ggtgatgtgg acctggcggc gcggtacccg gatcctgttc gagaagacgc ggcgctcgga
     2401 agtgccgctc aagggcctgg ctgacagcct ggcgaagcgt ccgccgcatc gcgtggccgg
     2461 tacggcggtg ttcttcacct ccgaccccga gagcgccccc acggcgctgc tgcacagtct
     2521 caaacactac agggtcctgc atgaggagaa cgtggtgctg accatccgca ccagcgacct
     2581 gccaacggtg ccggactcgg agaaggtgaa atacatgccg ctgaacgacc tgttcagttc
     2641 gctgatcgtc accttcggct acatggagac gccgcatgtg cccgaggcgc tggccctggc
     2701 gcgcaaggca ggatggaagt tcgacatcat gacgacgtca ttcttcctgt cgcgccgcaa
     2761 cgtgcgcgcg tcccggcacg aaggcatgcc gctgtggcag gaccagctct tcatcgccat
     2821 ggcgcgcaat gcggatgacg ccagccacta cttccgcctg ccgaccgacc gcgtggtgga
     2881 ggtgggcagc cagatcacgg tctaggccgc tacctgttca gcgcgtcgaa ccagtccttc
     2941 cactgcggca ccgcggttcc aaaggtgccg cggtggctgg tcttgccggt ggagatggcc
     3001 tcgacctgct gattgccgat cgccgtctga taggtcatcg cgagctggcc aaggccgatg
     3061 ctgatgacct cgtcggtttc gccatagtag ttgcgcaccg gcgttgcgat gacccagcga
     3121 taggcctggg ttgccgcaac cagccggcca taggccgagt cggcgaagaa ctgcgggtcg
     3181 aagtactccg cgcgaatcag cttgtgcaga tcggtgggta tgtcggcggg atcgaagggc
     3241 tcacgctcat aggccttgcg cgacacctcg tagtattcgt cgttgatcag ggatcgcgcg
     3301 aggccgggaa caccatagta gtgctcgaag gaaaaggccg agagaatgaa gagcgtggtg
     3361 acccacgagg catcaatggg ccgcgggaag ctgagaaagc cgttaagcgc cacgaagaca
     3421 tcgacgggcg cgcttgcggt ggcagcggcc ttcacctcga catgcgcctg ctcgagcctt
     3481 tccaggaagg ccatggtgac gaagccgccc tgcgaccaac ccccgagaaa gagatctccc
     3541 gcggtgagat tcagatgatc cagcacgacc cggctggcga ccagcatgtc gaagcaggcc
     3601 tgctgatggc tggccttgac gacgtagcct tcgtcgtcgt ccgaagtccc catgccgaaa
     3661 tagtcggcgc cgatgaccac atagccctgg cccgcgaact gcgcgatggc gagctgggtc
     3721 tccggcgatt gatccgggaa ggagggcacc tgctgctttc cgtagaccgt gccgtgctga
     3781 tacgacagca tcggcaggcc gttggccgcg ccctcgggca cggcgagaag tcccgaggcc
     3841 gtggtcggca ggttgccgcg ctcgggaatg accgagttgt aagtcacgcg atagagattt
     3901 actgccgatt gcgcagggct ataggcgatc ttgacgccgg taaaggccgg cgtgtcggtc
     3961 tgcaggatct ggttgagctt tttcacgtcc cagcgggcga gaaactcgta gctcacgccc
     4021 gatgcgacct ggatcggccc cttgtcctgc gccaaggcgg gtgtcgcgaa gacgcagacg
     4081 acgagtaaaa tcatgtggcg cagcatggcg gccatggcgg tctcctgttt cacaatccgg
     4141 acaagtcaga cggaccggga acgatgcggc gcaactgtga agatttcctg tatacggtgc
     4201 ggggcggggc gcgctacagc cgtgcgagcg tcacaaagcg gtcgatcatc gcattgacct
     4261 gatcgggcac ctcgagctgg cagaagtgcc ccgatccgac cgtctgtcca taccgcaggc
     4321 caggcaggag atcgaaaaga ccttccatgt cgcagcgcgg aaccggttcg tcggccgcga
     4381 tgtagagcgc cggaactttc acgcggccgc gggcagcgct cgaatcatag tcaccgagtc
     4441 cttcgaagga ggcagccgca acaaattgtg tggcctcgcc catgccctcc agaatcgctg
     4501 ccgagcggac cgcatcgtcc gtgggcagaa gaagcctctc cttcacatag gcgcgcagcg
     4561 tggcgacata gtccggacca cgcaggcgtt cgagaaaggc ggggatcgcc gcgcgcgtcg
     4621 cttcgggcag cacgatggcc gagtcgagca ttgcgattgc gccgggcaga tcaggatagc
     4681 gcgcggcgat gtcgaaggcc acgatgcccc ccatgttgtg accgatcagg atgggtttgg
     4741 agaggttcag cgcaccgcaa a
//
LOCUS       DBNGVR010000399         9171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2110407, whole genome shotgun sequence.
ACCESSION   DBNGVR010000399 DBNGVR010000000
VERSION     DBNGVR010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2110407"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="AC16A0_08470"
     CDS             complement(<1..814)
                     /locus_tag="AC16A0_08470"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR3105408.1"
                     /translation="MNAKRRIPSETTVAVLMGGWSAERQVSLSSGEACAGALRRAGFN
                     VVPIDVTRERIVSILQDLRPDVAFNALHGKWGEDGCCAAVLETLQIPYTHSGVLASSL
                     AMHKEKSKAIFREAGVPVAESKIVELEIVATQHPMPLPYVVKPIAEGSSVGVHIVNSS
                     SNGPAAIVLEQRDLFGTHAMVERFVPGKELTCAVVGDVALGVIDIVSKSSTFYDYVAK
                     YAPGGSQHILPAEIPNDVYRKCQQYAIMAHQALGCRGVSRADFRYNDQPGGEG"
     gene            complement(811..1740)
                     /gene="murB"
                     /locus_tag="AC16A0_08475"
     CDS             complement(811..1740)
                     /gene="murB"
                     /locus_tag="AC16A0_08475"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3105409.1"
                     /translation="MARLDGEALLKRLPAVRGRLEPEAALADLIWFRAGGPAEVLYTP
                     ADETDLADFLKAAPGDVAVYPIGVGSNLLVRDGGVPGVVIRMGKGFSGLTLEPGDRIR
                     AGAAVLDVRVARFAQEHGLDALTFLRGIPGTIGGALRMNAGAYGGETKDVLVEARAVD
                     RSGTIHVLSNADMKYTYRHCGAPEDLIFTSALLQGRPGDRAAIAAAMDKITESREATQ
                     PVKSRTGGSTFKNPPGRKSWQLIDQAGMRGFAVGPARVSEMHCNFLINEGGASASQIE
                     ALGEAVRAKVKTTSGIDLEWEIKRIGLKAGGTP"
     gene            complement(1740..3158)
                     /gene="murC"
                     /locus_tag="AC16A0_08480"
     CDS             complement(1740..3158)
                     /gene="murC"
                     /locus_tag="AC16A0_08480"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3105410.1"
                     /translation="MKLPRDVGPIHFIGIGGIGMSGIAEVMATLGYTVHGSDLTDNYN
                     VARLRKSGIAVEIGHRAENIGDAQVVVVSSAVKPDNPELIEARNRYLPIVLRAEMLAE
                     LMRFKTCVAVGGTHGKTTTTSLVAALLDAGGLDPTVINGGIINAYGTNARFGKGDWMV
                     VESDESDGTFVKLPADVVIVTNIDPEHLDHYGSFDKAKEAFLAFVENIPFYGFAVMCM
                     DHPVVQEIIGQVRDRRVITYGRSPQADVRLLETSYAEGETHFAVRITNRRTGATHDIG
                     DLSLAMPGDHNALNSTAALAVARELGLSDDQIRKGFKSFTGVKRRFTRTGEHNGITVF
                     DDYGHHPVEIAAVLRAARGVTQNRVVAVMQPHRYTRLSSLFNDFCTCFNDADTVIVAP
                     VYAAGEAPIEGASHLALVEGLRARGHRNVHAIETPDQLAPLVRNLVTPGDIVICLGAG
                     TITQWAYALPGQLAAFDGEAGA"
     gene            complement(3155..4309)
                     /gene="murG"
                     /locus_tag="AC16A0_08485"
     CDS             complement(3155..4309)
                     /gene="murG"
                     /locus_tag="AC16A0_08485"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3105411.1"
                     /translation="MRAPIARELSLRSTLKPGMMEKGTIVLAAGGTGGHLFPAQALAE
                     ELTRRGYVIHLMTDERVRDYGKSFPAQQTHVVPSATLSLGKPWLLPGRLFRLFGGYRQ
                     ARAIMQSLRPQAVVGFGGYPSFPPILAAANLKIPSCVHDQNAVMGRANRVLARYADAI
                     ASSFPDLQGLPPAAAHKVTVTGNPVRDLVLKSRAAAFPASGRFRLLVFGGSQGAQFFG
                     DFMPRVFAAMTADERAGLQVTQQVRPENMETVKAAYGQLNLDCELQPFFMDMPSRIAA
                     SHLVVCRSGASTIAELGVIGRPAVMVPLPHALDNDQLRNAESFASAGAGWVHPQAGLE
                     PQGFAAFLTRLRSQEGVLRKAAAAALGHGKPDAAQRLADLTEQLAGKAKE"
     gene            complement(4263..5402)
                     /gene="ftsW"
                     /locus_tag="AC16A0_08490"
     CDS             complement(4263..5402)
                     /gene="ftsW"
                     /locus_tag="AC16A0_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR3105412.1"
                     /translation="MLGRSDRGLLAQWWFTVDRGLMMAVILLMATGVLISMAASPPVA
                     DRIGLDTFHFFRSQLFYLFPAVLVLIGVSFLTPRQTRRAGFLLFAGSLALMVAALFWG
                     PEIKGAHRWINLGPLNLQPSELAKPGFVIVTAWLLAEQTRRPDIPAHFAAFLAAGLFF
                     GLLLMQPDFGQTALMIMAFGAMLLIWGLPWILVAGFAGLVASGILIAYASVAHVRSRI
                     DRFLDPDKGDTFQVDTAMQAFKNGGLMGAGPGGGEAKLILPDAHTDFTFAVVGEEFGL
                     VACLVLMSLFAFIVLRILARAKLETDPFPSLALSGLALVFGFQAIINMGVNVALLPAK
                     GMTLPFISYGGSSLIGMAIAMGFVLSLARRHAGAHRTRTLAAVYA"
     gene            complement(5389..6807)
                     /gene="murD"
                     /locus_tag="AC16A0_08495"
     CDS             complement(5389..6807)
                     /gene="murD"
                     /locus_tag="AC16A0_08495"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3105413.1"
                     /translation="MFPARSFRGRKLAVFGLARSGTACIEALRLGGAAVHAWDDSQPA
                     VEKARAAGLPISNLHELDFGTLDALVLSPGVPLTHPEPHWTVLKARHAGIEVIGDTEI
                     FAREAMAAGARIVAITGTNGQSTTTALAGHVLAAGGLDVDVGGNIGKAVFLLRQPVKD
                     RVYVLELSSFQIDLMPGLAPRVGILTNLTPDHLDRHGSMENYAAVKARMFARQGAGDT
                     SLCGVDDAWCSEIADSIAGGSDLRRVSVMRPLDDGIAAPEGVLRDRRGGTLVCEIDLR
                     DMPALRGQHNWQNACMAYGAAAAFGIAPGIIARAMQSFPGLAHRMQQVGAAGGIAFVN
                     DSKATNADAAEKALLSFDNIYWILGGIAKSGGIAPLRPHFGRVARAYLIGQSSDDFAA
                     ALEGQLPYTRCGTLDRAVAAALDDAQRDGRKGAVVLLSPACASFDQYPNFEVRGDAFV
                     KAVAGLPGVTMTITGDDNAAWT"
     gene            complement(6812..7900)
                     /gene="mraY"
                     /locus_tag="AC16A0_08500"
     CDS             complement(6812..7900)
                     /gene="mraY"
                     /locus_tag="AC16A0_08500"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3105414.1"
                     /translation="MLFYLLTSFADEVSVFNVFKYQTTRTGAAILTALLFVFLFGPRI
                     ISLLKVKQGRGQPIRSDGPQRHIVEKQGTPTMGGLMILSGIFVSTVLWADLGNLYVWA
                     VLFVTIGFGAIGFYDDFLKVTKSSTKGFSGKLRLLTEFAIAGIAVACFMWIGDRPLSS
                     SLAVPFFKDLLIPFGMFFIVVGMLVVVGAGNAVNITDGLDGLAIVPVMIAAASLGLIA
                     YLVGNFNFSNYLELHFVRGSGELAIMCGAIVGAGLGFLWFNAPPAMIFMGDTGSLSLG
                     GALGAIAVAAKHEIVLAIIGGLFVLETASVIIQVASFKLTGKRVFAMAPLHHHFEQKG
                     WKEPTIVIRFWIIAIVLALIGLSTLKLR"
     gene            complement(7900..>9171)
                     /locus_tag="AC16A0_08505"
     CDS             complement(7900..>9171)
                     /locus_tag="AC16A0_08505"
                     /inference="COORDINATES: protein motif:HMM:NF010693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HIR3105415.1"
                     /translation="DGHDFVAAALKAGAGLAMVSRPTEEMRAAGPLLVVAEDPLRGLE
                     NIGRAARARSHAQIIGVTGSVGKTSTKEMLRVALSASGETHASAASFNNHWGVPLTLA
                     RMPLEAAFGVFEIGMNHAGEITPLVGMVRPHVAIVTTIAASHLGHFNSLDEIADAKAE
                     IFTGVVPGGTAVINRDTPYFNRLSTAARATGIGRIVSFGKHAEADVRIERAVLHPDCC
                     CITANVMGETLIYKLGLPGEHMAVNSLAVLAAAKLTGADLARAALALATAEPAKGRGV
                     REELATPDGSLLLIDESYNANPASMRAALALLANARPGKNGRRIAVLGDMLELGTTGP
                     ELHADLAGPVDEAGVDVLYAAGPLMANLWGRIPSAKRGAYGETSAAITEAVLGGLRVG
                     DVVMVKGSLGSRMGPLAEAMRQKFPPVRKES"
BASE COUNT         1714 a         2962 c         3020 g         1475 t
ORIGIN      
        1 cgccctcgcc accaggctga tcgttgtagc ggaaatcggc acgcgacacg ccgcgacacc
       61 caagcgcctg atgggccatg atcgcgtatt gctggcactt gcggtagacg tcattcggaa
      121 tctcggcggg caggatatgc tgcgagccac cgggcgcgta cttcgcgaca tagtcgtaga
      181 aggtcgaact cttcgacacg atgtcgatga cgcccagggc cacgtcacca accaccgcgc
      241 aggtcagctc cttgccggga acaaagcgct cgaccatggc atgggtacca aacagatcgc
      301 gctgctccag cacgattgcc gccggcccgt tggaggaact gttcacgatg tggacgccca
      361 cgctcgaacc ttcggcgatc ggcttcacca catagggcaa gggcatggga tgctgcgtcg
      421 ccacaatctc gagttccacg atcttgcttt ccgccacagg cacgccggcc tcgcggaaga
      481 tggccttgga tttctccttg tgcatcgcaa gcgacgaggc gagcactccg gaatgcgtat
      541 agggaatctg caatgtctca agcacggcag cacagcagcc atcctcgccc cacttgccat
      601 gcagggcgtt gaaggctacg tcaggccgca agtcctgaag gatggacacg atgcgctcgc
      661 gcgttacgtc gatggggacc acattgaagc cggcacggcg gagcgcgcct gcacaggcct
      721 cgcccgacga caggctgacc tgccgttcgg ccgaccatcc gcccatcagc acggcgacgg
      781 tcgtttcgga aggaatgcgc cgcttcgcat tcatggcgtg cctcccgcct tgaggccgat
      841 acgcttgatc tcccactcga gatcgatgcc ggaggtggtc ttgaccttgg cgcgcacagc
      901 ctcgcccagg gcttcgatct ggctggcgct cgcaccgcct tcgttgatga ggaagttgca
      961 gtgcatctcc gatacccgcg ctggcccgac ggcaaagccg cgcataccgg cctgatcgat
     1021 cagctgccag gatttccgac ccggcggatt cttgaacgtc gaaccgcccg tcctgctctt
     1081 gaccggctgg gtcgcttcgc ggctctcggt gatcttgtcc atcgcggcgg cgatggcggc
     1141 ccggtcacct gggcggcctt gcagcagcgc gctcgtgaag atcaggtcct ctggcgcgcc
     1201 gcaatggcga taggtgtatt tcatgtcagc gttggacagc acgtgaatcg tccccgagcg
     1261 atcgaccgca cgcgcctcga ccagcacgtc cttggtttct cccccatagg cgccggcgtt
     1321 catgcgcaat gcgccgccga tggtgcccgg gatacctctc agaaaggtta acgcatctaa
     1381 accatgttcc tgtgcgaaac gggcgacacg cacgtccagg acggcagctc cggcccggat
     1441 gcgatcgccc ggctccagcg tcaggcccga gaatcccttg cccatgcgga tcaccacgcc
     1501 cggcacgccg ccgtcgcgca ccagaaggtt cgagcccaca ccgatgggat agaccgctac
     1561 gtcgcccggc gcagccttga ggaaatccgc aagatcggtt tcgtccgcgg gggtatagag
     1621 gacttctgcc gggccaccgg cacggaacca gatcaggtcc gccagtgccg cttcgggctc
     1681 cagccggccg cgcacggccg gcagccgctt cagcagcgcc tcgccatcaa gccgcgccat
     1741 cacgcacctg cttcgccatc gaaggccgcc agttggcccg gcaaggcata ggcccattgg
     1801 gtaatggtgc cggcgcccag gcagatcacg atgtcgcccg gcgtcaccag atttcgcacc
     1861 agcggcgcga gctgatccgg cgtttcgatg gcatggacat tgcggtggcc ccgcgccctc
     1921 agcccctcga ccagagccag gtggctggcg ccttcgatcg gcgcttcgcc tgcggcatag
     1981 acaggcgcca cgatcactgt atcggcatca ttgaagcagg tgcagaaatc gttgaacagc
     2041 gaggacagcc gggtgtagcg atgcggctgc atcacggcga ccacccggtt ctgggtgacg
     2101 ccgcgcgccg cgcgcagaac ggcggcgatc tcgaccgggt gatggccgta gtcgtcaaag
     2161 acggtgatgc cgttgtgctc gccagtgcgg gtgaaccggc gcttcacgcc agtgaaactc
     2221 ttgaagccct tgcggatctg gtcatcgctg aggccgagtt cccgcgccac ggccagcgcg
     2281 gccgtcgaat tcagggcgtt gtgatcgccc ggcatggcga ggctcagatc gccgatgtcg
     2341 tgggtagcac cggtccggcg gttggtgatc cttaccgcga agtgcgtctc gccttcggca
     2401 taagacgtct cgagcagccg gacatcggcc tgcggactac ggccataggt gatgacgcgg
     2461 cgatcgcgca cctggccgat gatctcctgc accaccggat gatccatgca catcaccgcg
     2521 aaaccgtaga acgggatgtt ctcgacgaag gcgaggaagg cctccttggc cttgtcgaag
     2581 ctgccgtagt ggtcaaggtg ctcggggtcg atattggtga cgatcaccac atccgccgga
     2641 agcttgacga aggtgccatc ggactcgtcc gattccacca ccatccagtc acccttgccg
     2701 aaccgcgcat tggtgccata ggcgttgatg atgccaccgt tgatcacggt agggtcgaga
     2761 ccgcccgcgt cgagcaaggc ggcaaccagt gaggtggtgg tggtcttgcc gtgggttcca
     2821 ccgaccgcca cgcaggtctt gaaacgcatc agctcggcca gcatctcggc ccgcagaacg
     2881 atgggaagat agcggttgcg cgcctcgatc agttccggat tgtcaggctt caccgcggag
     2941 gagacgacaa caacctgcgc gtctccgatg ttctcggcgc ggtgaccgat ctccacggcg
     3001 atgccgctct tgcgcagccg cgccacgttg tagttgtcgg tcaggtccga gccatgcacc
     3061 gtgtagccca gcgtcgccat gacttcggcg atgccgctca tgccgatgcc gccgatgccg
     3121 atgaaatgaa tggggcctac gtcgcgcggc agtttcattc tttggctttc cctgcaagct
     3181 gttcggtcag atcggcgagg cgctgggcgg catccggctt gccatggccg agtgcagcgg
     3241 ccgccgcctt tcgcaacacg ccctcttgag agcgcaggcg tgtcagaaat gcggcaaagc
     3301 cctgaggctc caggccggcc tgtggatgga cccagccggc gcctgccgat gcgaaactct
     3361 cggcattgcg caactggtcg ttgtccagcg catgcggcag cggcaccatg acggccggcc
     3421 ggccgatgac gcccagttcg gcgatggtgg atgcaccgga gcggcagacc accagatgcg
     3481 acgccgcgat ccgggagggc atgtccatga agaagggctg cagctcgcag tcgaggttca
     3541 actgcccata ggccgccttc acggtctcca tgttctcggg gcgaacctgc tgcgtcacct
     3601 gaaggccggc ccgctcatcg gcagtcatgg cggcgaagac cctgggcatg aagtcgccga
     3661 agaactgtgc gccctggctg ccgccgaaga ccagcaaccg gaagcgccca gatgccggaa
     3721 aggcggcggc gcgggatttc aggaccagat cgcgtacggg atttccggtg accgtcacct
     3781 tgtgcgcagc agccggagga aggccctgca gatcggggaa ggacgaggca atcgcatcgg
     3841 cgtatctggc cagcaccctg ttggcccggc ccatgacggc attctggtcg tggacgcagg
     3901 aggggatctt cagattggcg gcggccagga tcggcgggaa ggacggatag ccgccaaaac
     3961 ccaccacggc ctgcggccgc agcgactgca tgatcgcccg cgcctgacga tagccgccga
     4021 acaggcggaa cagcctgccc ggcagaagcc aaggcttgcc gagcgagagg gtggccgacg
     4081 gcacgacgtg agtctgctgg gccgggaagg acttgccata gtcgcgcacg cgttcgtccg
     4141 tcatcagatg gatgacgtag ccgcggcgtg tcagctcctc ggccagggcc tgggccggga
     4201 agaggtggcc gccggtgccg ccggcggcca gaacgatggt gcctttctcc atcatgccgg
     4261 gcttaagcgt agaccgcagc gagagttcgc gtgcgatggg cgcccgcatg gcggcgcgcc
     4321 agcgaaagca cgaagcccat ggcgatggcc atgccgatga gggaggagcc gccataggag
     4381 atgaagggaa gagtcattcc cttcgccggc agcagggcca cgttcacacc catgttgatg
     4441 atggcctgga aaccaaagac gagcgccagg ccgctgaggg ccagggacgg gaagggatcg
     4501 gtctccaact ttgcacgcgc caggatgcgc agcacgatga aggcaaagag cgacatgagg
     4561 acgaggcagg ccaccagccc gaattcctcg ccaaccacgg cgaaggtgaa gtcggtatgg
     4621 gcatccggga gaatcagctt cgcttcgccg ccgcccggtc cggcgcccat gagaccgcca
     4681 ttcttgaagg cctgcatcgc cgtatcgacc tgaaaggtgt cgcccttgtc ggggtcgagg
     4741 aagcggtcga tgcgcgaacg cacatgcgcc acgctggcat aggcgatgag aatgccagac
     4801 gccaccagcc ccgcgaagcc agcgaccaga atccacggca gaccccagat cagcagcatg
     4861 gcgccgaagg ccatgatcat cagggccgtc tggccgaagt ccggctgcat cagcagcagc
     4921 ccgaagaaga gtcctgcggc aaggaaggcc gcaaaatgcg ctggtatgtc cggacgccgc
     4981 gtctgttccg ccagcagcca ggcggtgacg atgacgaagc ccggctttgc cagctcagag
     5041 ggttgcagat tgagagggcc gaggttgatc cagcgatgcg cgcccttgat ctcggggccc
     5101 cagaacagcg ccgcaaccat caaggcaagg cttcctgcga agagcaggaa gccggcgcgg
     5161 cgggtctgcc gcggcgtgag gaacgacacg ccgatcaaca ccagcaccgc agggaagaga
     5221 tagaagagct ggctccggaa gaaatgaaaa gtgtcgaggc cgatgcgatc cgcaaccggc
     5281 gggctggccg ccatgctgat cagcaccccg gtggccatga gcaggatcac cgccatcatc
     5341 aatccgcggt cgacggtgaa ccaccattgg gcgagaagcc cacggtcgct acgtccaagc
     5401 agcattgtca tctcctgtga tggtcatggt gacaccggga agcccagcga cggccttgac
     5461 gaaggcatcg ccgcgcacct cgaagttggg atactggtcg aaggaggcgc aggcgggcga
     5521 gaggagcacg accgcgccct tccgcccatc gcgctgcgca tcgtcaagcg ccgcggcgac
     5581 cgcgcgatcc agcgtgccgc acctggtata ggggagctgc ccctccagcg ccgcggcaaa
     5641 atcatccgag gactggccaa tgagataggc gcgtgccacg cggccgaagt gcggacgcag
     5701 cggcgcaatg ccgccggatt tcgcgattcc gcccaggatc cagtagatgt tgtcgaagga
     5761 caagagcgcc ttctccgccg catcggcatt ggtggccttg gagtcgttga cgaatgcgat
     5821 gccgccggcc gctcccacct gctgcatgcg gtgggcaagg cccggaaagc tctgcatggc
     5881 ccgcgcgatg atgccgggcg cgatgccgaa ggccgctgca gcgccatagg ccatgcaggc
     5941 attctgccag ttgtgctgcc cgcggagcgc cggcatgtcg cgcaggtcga tctcgcagac
     6001 gagagttccg ccgcgccggt cgcgcagcac gccctcgggt gccgcgatgc cgtcatcgag
     6061 cggccgcatc actgacacgc ggcggaggtc ggaaccacct gcgatgctgt ccgcgatctc
     6121 gctgcaccat gcgtcatcga caccgcagag cgaggtatcg ccagcgcctt gcctggcgaa
     6181 catgcgggcc ttgacggcgg cgtagttttc catgctgccg tgccggtcga gatggtcggg
     6241 cgtcagattg gtcaggatgc cgaccctggg cgcaagcccg ggcatcaggt cgatctggaa
     6301 ggacgacagc tcgaggacat agacccggtc cttcaccggc tggcgcagca ggaacaccgc
     6361 cttgccgatg ttgccgccga cgtcgacgtc gagaccgccg gcagccagca cgtgaccggc
     6421 cagcgcggtt gtcgtcgatt ggccattggt gccggtgatg gccacgatgc gggcgcccgc
     6481 ggccatggct tcgcgcgcga agatttcggt atcgccgatg acctcgatcc cggcatgcct
     6541 cgccttgaga acagtccagt ggggttcggg atgggtgagc ggcacgccgg ggctcagcac
     6601 cagggcatca agcgtgccga agtcgagctc gtgaagattc gagatcggca accccgcagc
     6661 ccgcgccttc tcgacggccg gctgggaatc atcccaggcg tgaaccgcgg cgccgccaag
     6721 ccgcagcgcc tcgatgcagg ccgtgcccga gcgcgcgagt ccgaagaccg cgagcttccg
     6781 tcccctgaat gacctggccg ggaacacgct tctacctgag cttgagcgtc gacagaccga
     6841 tcagcgccag gacgatggcg atgatccaga agcggatgac gatggttggt tccttccagc
     6901 ccttctgttc gaagtgatga tgcagcggcg ccatggcgaa gacgcgcttg ccggtcagtt
     6961 tgaaggaggc gacctggatg atgaccgagg ccgtctcgag gacgaacagg ccgccgatga
     7021 tggcgagcac gatctcgtgc ttggccgcaa cggcaatggc accgagcgcg ccgccgagcg
     7081 acagcgaacc ggtgtccccc atgaagatca tggccggcgg ggcgttgaac cacaggaatc
     7141 ccagtcctgc cccgacgatg gcgccgcaca tgatggcgag ctctcccgag ccgcggacga
     7201 agtggagttc aagatagttc gagaagttga agttaccgac gaggtaggcg atcagcccga
     7261 gacttgccgc cgcgatcatc accggaacga ttgcgagtcc gtcgaggccg tcggtgatgt
     7321 taactgcgtt gccggcgccc acgaccacca gcatcccgac cacgatgaag aacattccga
     7381 atgggatgag caggtccttg aagaacggga cggcgaggga actcgacagc ggccggtcgc
     7441 cgatccacat gaagcatgca accgcaatgc ccgcgatcgc gaattctgtc agcaggcgaa
     7501 gcttgccgga gaaacccttg gtcgatgatt tggtgacctt caggaagtcg tcatagaagc
     7561 cgattgcacc gaagccgatg gtcacgaaga gcacggccca gacatagaga ttgccgagat
     7621 cggcccacag cacggtggat acgaagatgc ccgacaggat catcagtccg cccatggtcg
     7681 gcgtgccctg tttttccacg atgtggcgct gcggtccatc gctgcggatc ggctgacccc
     7741 tgccctgctt caccttgagc aaggagatga tgcgcggacc gaagaggaag acgaagagca
     7801 atgccgtcag gatcgccgca ccggtccgcg tcgtctggta cttgaagacg ttgaacaccg
     7861 acacttcgtc ggcgaaactg gtgagcaggt agaagagcat caggactcct tccggaccgg
     7921 cgggaatttc tgccgcatgg cctccgccag cgggcccatc cggctgccga gcgacccctt
     7981 gaccatcacg acatccccca ccctcagacc gcccaggacg gcttcggtga ttgcggctga
     8041 agtttcgcca taggctccgc gctttgccga tggaatcctg ccccagagat tcgccatcag
     8101 cggcccggcg gcatatagga catcgacacc ggcctcgtcc accggcccgg ccagatcggc
     8161 atgaagctcc ggtcccgtgg ttcccaactc cagcatgtcg cccaggacag caatccgccg
     8221 gccgttcttg cccggcctgg cattggccag cagcgccagg gcagcgcgca tggaggccgg
     8281 attggcgttg tagctctcgt cgatcagcag caggctgccg tcgggggtcg caagctcctc
     8341 gcgcacgccg cgccccttgg cgggttctgc ggtcgccagc gcgagggccg cacgggccag
     8401 atcggcgccg gtcagtttcg ctgcggcaag gaccgccagg ctgttgaccg ccatgtgctc
     8461 gccaggaagg cccagcttgt agatcagggt ttcgcccatg acgttcgcgg tgatgcagca
     8521 gcaatcggga tgcagcacgg cccgctcgat ccggacatcg gcttcggcat gcttgccgaa
     8581 ggagacgatc ctgccgatcc ccgtggcacg cgccgcggtg ctaaggcggt tgaagtaagg
     8641 ggtgtcgcgg ttgatcaccg cggtgccgcc cggtaccacg ccggtgaaga tctcggcctt
     8701 ggcatcggcg atctcgtcga gcgagttgaa atggcccaga tggctggcgg cgatggtggt
     8761 gacgatggcc acatggggcc tgaccatgcc gaccagcggg gtgatttcgc ccgcatggtt
     8821 catgccgatc tcgaagacgc cgaaggcggc ctccagcggc atgcgcgcca gcgtgagcgg
     8881 cacgccccag tgattgttga aggacgcagc cgacgcatgg gtctcgcccg aggcggacag
     8941 ggcgacgcgc agcatctcct tggtgctggt cttgcccacg ctgccagtga cgccgatgat
     9001 ctgcgcatgc gagcgggccc gggcggcgcg cccgatgttt tcgagaccgc gcaggggatc
     9061 ctcggccacg accagcagcg ggccggccgc ccgcatctct tccgtggggc gcgaaaccat
     9121 ggcaaggccc gccccggcct tcagggcagc cgcgacgaag tcatggccat c
//
LOCUS       DBNGVR010000400         2623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2700340, whole genome shotgun sequence.
ACCESSION   DBNGVR010000400 DBNGVR010000000
VERSION     DBNGVR010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2700340"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /gene="glpK"
                     /locus_tag="AC16A0_08510"
     CDS             <1..1258
                     /gene="glpK"
                     /locus_tag="AC16A0_08510"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR3105416.1"
                     /translation="GITNQRETVVIWDRRTGKPIHRAIVWQDRRTSDICARLKSEGHE
                     ELFASRTGLLLDPYFSGTKIAWILDKVKGARARAERGELAFGTIDSFLIWRFTAGRVH
                     ATDATNASRTLLYNIHSGSWDDELLRILNVPRSLLPDVRDCAADYGVTDPGLLGAAIP
                     ICGVAGDQQAATIGQACFEPGMLKATYGTGCFALLNTGTTAVASQNRLLTTVAYQLNG
                     QRTYALEGAIFIAGAAVQMLRDGLKIVRTADETGKLARSADPAQQVYLVPAFVGLGAP
                     YWNAEARGAIFGLTRGTTRAELAQAALEAVCYQTHDLLEAMKKDWGSAGRTILRVDGG
                     MTASDWTMQRLADILDAPVDRPKVLETTAVGAAYLAGLHSGLLPEPPVFAKSWKRQAR
                     FTPRMKPAIRSAKLAGWREAVRKLLS"
     gene            complement(1286..1801)
                     /locus_tag="AC16A0_08515"
     CDS             complement(1286..1801)
                     /locus_tag="AC16A0_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3105417.1"
                     /translation="MTRFTLSVLAAAALALAACSSSKQADLPPAAPVVQPSVPSTGGV
                     GAAPGSEQDFMLNVGRRVFFAEGSAALDSTARATIRNQVQWLKANPQWKAKIQGFADD
                     PGGDAQQTALSQKRADAVKNDMVSQGIAASRIWAKGYGRDRLVGDCAELECKAQNRRT
                     VTNLQDGTEAQ"
     gene            complement(1798..2478)
                     /locus_tag="AC16A0_08520"
     CDS             complement(1798..2478)
                     /locus_tag="AC16A0_08520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3105418.1"
                     /translation="MPINRRNFLMGAGALGLSGLTVDSGEAAGPRPKDASAYFGPMIE
                     DNGIVFPSTNFTKINVRWRRQFVPYTSREPQGTVVVDASNHFLYVIFENQTALRYGVG
                     VGRDGFRWYGRARIDRKQLWPRWTPPPAMRARNPDLPEFMKGGKDNPLGPRAMYLFRK
                     GTDTGYRIHGTTEPWSIGTNASSGCIRMFNEDAIDLYQRCPIGTRVLVLKALGAPVIN
                     EAAEVDSQ"
BASE COUNT          466 a          826 c          864 g          467 t
ORIGIN      
        1 gggcattacc aaccaacggg aaaccgttgt gatctgggac cgcagaacgg gaaagcccat
       61 tcaccgcgca atcgtctggc aggatcggcg aacgtcggac atctgtgcga ggctcaagtc
      121 cgagggccac gaagagctgt tcgcgtcacg gacaggcctg cttctcgatc cctacttttc
      181 gggcacgaag atcgcgtgga ttctggacaa ggtgaagggc gcacgggccc gggccgaacg
      241 tggtgaactc gccttcggca ccatcgacag ctttctgatc tggcggttca ctgcgggcag
      301 ggttcacgcc accgatgcca ccaacgcgtc tcggacgctg ctctacaaca ttcacagcgg
      361 tagctgggat gacgagttgc tccggattct gaatgtgccg cgcagcctgc tccccgacgt
      421 ccgcgactgt gccgctgact atggtgtgac cgatccgggt ctgctgggcg ccgcgatacc
      481 gatctgcggc gtggctggcg accagcaggc cgcaaccatc gggcaagcct gttttgagcc
      541 gggcatgctg aaggcgacct atggcaccgg ctgcttcgcc ctgttgaaca cgggcaccac
      601 tgcagtagcg tcgcaaaaca ggctcctgac caccgtggcc tatcagctca acggccagag
      661 aacctacgcg ctcgaagggg cgatcttcat cgccggcgca gcggtgcaga tgctgcgcga
      721 cgggctgaag atcgtgcgga cagccgatga gaccggtaaa ctggcccgca gcgccgaccc
      781 ggctcagcag gtctatctcg tgccggcctt cgttggcctt ggcgcgccat actggaatgc
      841 cgaagcccgc ggcgccatct tcggactgac ccgggggacg acgcgggccg agctagccca
      901 ggccgcgctc gaggccgtct gctaccagac ccacgatctc cttgaggcca tgaagaagga
      961 ctggggctcg gcggggcgaa ccatcctgcg cgtggacggc gggatgacgg cctcggactg
     1021 gaccatgcaa cgcctggccg acattcttga tgcaccggtc gaccggccga aggttctaga
     1081 gacgacggca gtgggcgcgg cctatcttgc cgggcttcat tctggtcttc ttcccgagcc
     1141 gccggtcttc gccaagagct ggaagcgtca ggcgcgcttc acccccagaa tgaagcctgc
     1201 gatccgttcg gccaagctgg cgggctggcg cgaagccgtg cgcaagctct tgtcgtagcc
     1261 gtagcgatgc ggggccgggc ggacgctatt gcgcttccgt tccgtcctgc agattggtga
     1321 ccgtccggcg gttctgcgcc ttgcattcga gttcggcgca gtcgcccacg agccggtcac
     1381 ggccatagcc cttggcccag atccggctcg cggcaatgcc ttgtgacacc atgtcgttct
     1441 tcacggcatc ggcacgcttt tgcgacagcg ctgtctgctg cgcgtcaccg ccgggatcat
     1501 cggcaaagcc ctggatcttc gccttccatt gcggattggc cttgagccac tgcacctggt
     1561 tgcggatggt ggcgcgcgcc gtgctgtcga gcgctgcgga tccttcggcg aagaaaacgc
     1621 ggcggcccac gttcagcatg aaatcctgct cgcttccggg agcggcgccg acgccgcccg
     1681 tgctgggaac cgagggctga accacgggag cagccggggg aaggtcggcc tgcttcgaac
     1741 tgctgcaggc cgccaaggcc agggccgctg cggcaagcac agacaacgta aagcgtgtca
     1801 ttgactgtcg acttccgcgg cttcgttgat gacaggcgcg cccagggcct tgagcacaag
     1861 cacgcgcgtg ccgatggggc agcgctggta gaggtcgatc gcatcctcgt tgaacatgcg
     1921 gatacagccg gacgaagcat tggtgccgat cgaccacggc tcggtggttc cgtgaattcg
     1981 atagccggta tccgtgccct tgcggaacag gtacatggcc cgggggccga gggggttgtc
     2041 cttcccgccc ttcatgaatt ccggaaggtc cgggtttcgg gcgcgcattg ccgggggcgg
     2101 ggtccagcgc ggccagagct gcttgcggtc gatgcgagcg cggccgtacc agcggaagcc
     2161 gtcacggcca acaccaacgc cgtagcgcaa ggctgtctga ttctcgaaga tcacgtagag
     2221 aaaatgattg ctggcatcta ccacgaccgt gccctgcggt tcgcggctgg tgtagggcac
     2281 gaactgccgg cgccagcgta cattgatctt cgtgaaattg gtggacggaa agacgatccc
     2341 attgtcctcg atcatcggac cgaaataggc gctggcatcc tttggccggg ggccggctgc
     2401 ttcaccggag tcgacagtca ggccggagag gcccagggct cccgcaccca tcaggaaatt
     2461 cctacgattg atcggcatgt ttcctccatc acccccgatg cggcgctccg gtcagagtcg
     2521 tggaccggag cgcgcggcgt gcaatctagc gtggaaaacc ggacctgtca tcgggcccgt
     2581 cagcagggga ctaagcaaca gattactcct gcagcaagag agg
//
LOCUS       DBNGVR010000401         2989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_736116, whole genome shotgun sequence.
ACCESSION   DBNGVR010000401 DBNGVR010000000
VERSION     DBNGVR010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_736116"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="AC16A0_08525"
     CDS             complement(<1..1025)
                     /locus_tag="AC16A0_08525"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR3105419.1"
                     /translation="MPNLIAGFLLFSGFAGLIYQVAWVRLIGQSLGATSVSVAIVISS
                     FFLGLALGSHLAGRMTWLRSRIATYALLEVCIGLSALVLLPILLNLDVVLALAPAFGS
                     SLVLKAALCLALLILPTTAMGATFPVAAALLENRSGHTGRQISRLYSLNTLGAVLGAA
                     LSGLWIIPEMGLDGAVYLAAAVNLAIAALAFALSRSGDGDPGTATLPALDTALRPAYR
                     TPALITLALTGVVSIAAQVGWTKFLVIYIGSTISGLSIILAVFLAGIAMGAWLMGRRI
                     ATLTSPAAVMVTLLAALPFALIASRFLLSLLPDIQAIMNRAGEDAGLLMFIRAATIAA
                     ALLPPTVL"
     gene            1303..1779
                     /locus_tag="AC16A0_08530"
     CDS             1303..1779
                     /locus_tag="AC16A0_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3105420.1"
                     /translation="MFEKFDAGLRRSLPEGAVLLQEGIKSGKLLILAEGRVEVRRGDT
                     LVAVIDEPGSVFGEMSALLDVPHTASVVAVTPVTVYVFDDANAYLHSDPEVSLTVARV
                     LAQRLNTVTTYLVDIKRQYASHSNHLGMVSEVLASLVYQQRAAFTPGSDRQPDPGM"
     gene            1957..>2989
                     /locus_tag="AC16A0_08535"
     CDS             1957..>2989
                     /locus_tag="AC16A0_08535"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3105421.1"
                     /translation="MKARSFIARRLASRGSWGRAAALMMLIAALALRISDPTPVELLR
                     LKTFDLYQLMVKPPDQPAQAVIVDVDEASLNRIGQWPWPRTLMAQLVNEIRAKGAAAI
                     AIDILFPEYDRLSPGAMADMLAGDDAAMAARLKALPSNDEKFAEAIAASQVVLGQAVH
                     QSKNTQWHAEAREQTGFAMVGPSPDAFLDTYPGLVRNVPELEHAAAGLGLVSVRPDID
                     GIIRKVPLMLKADDRVLPSLGLDLLRVATGESTILLRSDEGGVTSVVLSGVILPTDES
                     GHAWLHFRPHNPDIFISAADVIDGKLPYGILQDRLVIIGSSATGLFDLKATPVDAVMP
                     GAEIHAQLVS"
BASE COUNT          543 a          954 c          998 g          494 t
ORIGIN      
        1 agcacagtcg gcggcagcag cgcagccgcg atggttgcgg cacggatgaa catcaacagg
       61 ccggcatcct ctccggcccg gttcatgatc gcctggatgt ccggcaagag gctcagcagg
      121 aagcggctgg cgatcagggc gaagggaagg gcagccagca gggtcaccat tactgcggca
      181 ggggacgtca gcgtcgcgat gcgtcggccc atgagccagg cccccatggc gatgccggcg
      241 aggaagaccg cgagaatgat cgaaagtccg gagatcgtgc tgccgatgta gatgaccaga
      301 aacttggtcc atcccacctg cgcggcgatc gagaccacgc cggtcagcgc cagtgtgatc
      361 agggccggcg tgcggtaggc cgggcgcagt gctgtgtcta gggcggggag cgtcgccgtc
      421 ccgggatcgc catcgccgct gcgggacagc gcaaaggcga gggctgcgat ggcgaggttg
      481 accgcagctg ccagatagac cgcgccatcg agtcccattt ccgggatgat ccacagccct
      541 gacagggccg cgcccaggac ggctcccagc gtgttgaggc tgtagagcct gctgatctgc
      601 cgtccggtat ggccgctgcg gttttccagc agtgcggcgg cgacggggaa tgtcgcaccc
      661 atggcggtgg tcggcaggat cagcagagcc aggcagagcg ccgccttcag gacgaggctg
      721 gacccgaagg ccggcgccag cgccaggacc acgtcgaggt tcagcaggat cggcagcagg
      781 acgagggccg aaagcccgat gcagacctcg agcagggcat aggtggcgat ccgggaccgg
      841 agccaggtca tgcgcccggc cagatggcta cccagggcga ggccgagaaa gaaggaggag
      901 atgacgatgg caaccgatac gctggtcgcg ccaaggctct ggccgatcag cctcacccag
      961 gcgacctgat agatcaatcc cgcgaagccc gagaacagca ggaaaccagc aatcagattc
     1021 ggcatgccaa gcattcgtcg cggtcgcaga tcggccgtca cttaaccgtc aacaggcgca
     1081 gcctggcaac agcattcgct gcccccgttt gggaaagtcc atgcgccgct gggttgcagt
     1141 cggtccagaa gcaagccctt catattctgc acaatttatg ggcagcgtcg atggctctgt
     1201 gtggtacagg gacccaaggt tgagattgcg gcggcagcgg tgcttacagt aaacttctct
     1261 gacgcaatgc agcatgaacg gagtcccgca cagatggcgc cgatgttcga gaagtttgac
     1321 gcgggattga ggcgaagcct gcctgagggg gccgtgcttc ttcaggaagg catcaagtcg
     1381 ggcaagttgc tcatcctcgc ggagggccgc gtcgaggtcc ggcgtggcga cacgctggtg
     1441 gccgtcattg acgagccggg ctcggtcttc ggcgaaatgt cggcgctgct cgatgtcccc
     1501 catacggcct cggtggtggc cgtgacgccg gtcaccgtct atgtcttcga cgacgccaat
     1561 gcctatctcc actccgatcc ggaggtctcg cttaccgtcg cccgcgtgct ggcgcagcgg
     1621 ttgaacacgg tgaccaccta cctggtcgac atcaagcgcc agtatgccag ccacagcaat
     1681 catctcggca tggtcagcga ggtactggcc agcctcgtct accagcagcg cgcggccttc
     1741 acgccgggct ccgaccggca gcccgatccg ggcatgtagc gggggctttg ctgcacggct
     1801 gccggcggac tggtgccgcg ccagggaaac ggctaaaccc ggctggggcc acaggcgaca
     1861 cacggcggcg aggcagctcg ggatggacgc gccagcattg acttcctgct ctgcggcacg
     1921 gcagaggcgc agcgccattg cgcagggctg tatcggttga aggcccgttc cttcatcgcc
     1981 aggcggcttg caagccgcgg cagctggggc cgcgccgctg cgctgatgat gctgattgcc
     2041 gcgcttgccc tgcgcatctc cgatcccaca ccggtggaat tgctgcggct caagaccttc
     2101 gatctctatc agctcatggt gaagccgccg gatcagccgg ctcaggccgt catcgtcgac
     2161 gtcgacgaag ccagtctcaa ccgtatcggc cagtggccgt ggccgcgcac gctcatggcg
     2221 cagctggtca atgaaatcag ggccaagggc gctgccgcca tcgccatcga tatcctgttc
     2281 ccggagtatg accgtctctc gcccggggcc atggcggaca tgctcgcggg ggacgatgcg
     2341 gcgatggccg cgcggctgaa ggcccttccg tccaacgacg agaaattcgc agaggcgatc
     2401 gccgcctccc aggtcgttct ggggcaggcc gtgcaccagt ccaagaacac gcaatggcat
     2461 gccgaggccc gcgaacagac gggctttgcc atggtcggtc cgtccccgga cgcctttctc
     2521 gacacctatc cgggtcttgt gcgcaatgtg ccggaactcg aacacgccgc ggccggcctg
     2581 ggcctggtat cggtccgccc cgacatcgat ggaatcatcc gcaaggttcc gctgatgctg
     2641 aaggccgatg accgcgtcct gcccagtctc ggactcgatc tcctgcgggt cgcgacaggc
     2701 gaaagcacca tcctgctgcg cagcgatgag ggcggcgtca cctcggtggt gctttcgggc
     2761 gtcatccttc cgaccgacga atccggccat gcctggctgc atttccgtcc gcacaatccc
     2821 gacatcttca tctcggccgc cgacgtgatc gatggaaagc taccctacgg gattctgcag
     2881 gaccgtctgg tgatcatcgg ctcctcggcc accgggctct tcgatctcaa ggccacgccc
     2941 gtggatgcgg tcatgccggg cgcagagatt catgcgcagc tggtctcgg
//
LOCUS       DBNGVR010000402         3333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1915615, whole genome shotgun sequence.
ACCESSION   DBNGVR010000402 DBNGVR010000000
VERSION     DBNGVR010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1915615"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..359
                     /locus_tag="AC16A0_08540"
     CDS             120..359
                     /locus_tag="AC16A0_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3105422.1"
                     /translation="MTETVTEICKLLEPFNTVGATLSPSTDISTDLNIDSVTVMDFVM
                     EVEDHFDIEIPLNVLSETRTIADLAKVVEARAKKA"
     gene            363..1574
                     /gene="spt"
                     /locus_tag="AC16A0_08545"
     CDS             363..1574
                     /gene="spt"
                     /locus_tag="AC16A0_08545"
                     /EC_number="2.3.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF047599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004758 - serine C-palmitoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046513 - ceramide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine palmitoyltransferase"
                     /protein_id="HIR3105423.1"
                     /translation="MTDLFDKHRHIAERHQRMLQLGVNAVGVTNQRLLSPTRAIIDGR
                     ETILAGTNNYMGITFDPDCIAAGKGALDQFGTGTTGSRIANGSYSLHKDLEAELARFL
                     DRKHCIVFTTGYQANLGMMSGLAGPRDTIYLDADSHSSIYDGCTLSGAKLVRFRHNDA
                     ADLDRRLARGEEDGGGRLVVLEGIYSMLGDRAPLADFIEVKKKHGFQLLVDEAHSFGV
                     LGPKGRGLADEAGLEDEVDFVVGTFSKSVGAIGGFGAGNHPLFEYLRYAMRPYMFTAS
                     SSPASIATSIAAIRKLAAEPQRRDRLRENSARLFHGFKALGLDMGCDTVSPVIAVKCP
                     DEPSTIAMWNALLKAGVYVNIALPPGTPNKLCLLRCSVSAAHTDADIDRIITLFDSVV
                     AQGHGKKVVNG"
     gene            complement(1571..2428)
                     /locus_tag="AC16A0_08550"
     CDS             complement(1571..2428)
                     /locus_tag="AC16A0_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105424.1"
                     /translation="MNAAETLLARSLEQPVPPEIAAMASHVRSAHDGVMAVLAYGSCL
                     RGVATTDSLIDLYVLTRDLRGVSANVLSRLGCRLIPPNVYYAECDIGGQRYRAKYAVL
                     PMPLFAVGMTRSNPYFWARFAQPSALVFAADAEARRDVISAAARAAQTMFAHARALSP
                     MGDPLDIWAAGFRATYATELRAEAGDRADQLVAANESYYRDLAAALAATAAQPASWGW
                     KRASGKLWAAARLIKASFTFSGGADYLVWKIERHSGQKIAISPWQRRHPVIAGLMLLP
                     KLLSSKAVR"
     gene            complement(2429..>3333)
                     /locus_tag="AC16A0_08555"
     CDS             complement(2429..>3333)
                     /locus_tag="AC16A0_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR3105425.1"
                     /translation="GLALAGMLRQTGNVSIKIIDRFDMIEGLVDELATEGVSDLFISS
                     GDGTIQAIQTILAERRPFPRLPRLALLPHGTTNMTAADLGFHHRSLAAQADFIRELKH
                     TDLRARPTVRCVNPRDGSPRHGMFVGCGAVADATRFTQIAFNAKGVKGGYGVFATLTG
                     SVIRSLFTKPDPTDTTRFDRPYPVRVEAEGHRYADGNQLLMLATTLEKLVLNTRPFWG
                     GKTGPIRVTTFPYPVPSIPRWLLPMMYGGEDRKFPQGAVSFCTPALEVTTPVTFVIDG
                     EFFEPPVGEPLRLEAGPVFTYVCR"
BASE COUNT          611 a         1049 c         1090 g          583 t
ORIGIN      
        1 ccgatccgat ttgcgacagg ccttccggag acgctggcgt ggtatcgcca ggccggatgg
       61 ttgcctcggg caggcggcac cgatagaagc ggggccatat cgaagaagga agccggaccg
      121 tgaccgagac cgttaccgag atctgcaagc tgctggaacc gttcaacacg gtcggcgcca
      181 cgctttcgcc ttcgaccgac atttccaccg atctcaacat cgattccgtc acggtgatgg
      241 attttgtgat ggaggtcgag gaccatttcg acatcgagat tccgctgaac gtcctttccg
      301 aaacccgcac catcgccgac ctcgccaagg tcgtcgaggc ccgcgcaaag aaggcctgat
      361 ccatgaccga cctcttcgac aagcacaggc atatcgcaga gcgccaccag cgcatgctgc
      421 agctgggcgt caatgccgtg ggggtgacca accagcgcct gctgtcgccc acgcgcgcca
      481 tcatcgatgg ccgcgagacc attctggccg gcaccaacaa ctacatgggc atcaccttcg
      541 accccgattg catcgcggcc ggcaagggcg cgctcgacca gttcggtacg ggcaccacag
      601 gctcgcgcat cgccaacgga tcctacagcc tgcacaagga tctcgaagcg gaactcgcgc
      661 gctttctcga ccgcaagcac tgcatcgtct tcaccaccgg ctaccaggcg aacctcggca
      721 tgatgtcggg cctcgcgggt ccgcgcgaca cgatctatct ggacgccgat tcccattctt
      781 ccatctatga cggctgcacc ttgtcgggcg ccaagctggt gcggttccgc cacaatgatg
      841 cggccgatct cgacaggcgc cttgcgcgcg gcgaggaaga cggtggcggc cggctcgtgg
      901 tgctggaagg catctactcg atgctgggcg accgcgcccc cctcgccgat ttcatcgagg
      961 tcaagaagaa acacgggttc cagcttctgg tggacgaggc gcattccttc ggcgtgctgg
     1021 gaccgaaggg acgcggtctc gccgacgaag cggggctcga ggacgaggtt gatttcgtgg
     1081 tcggcacctt ttccaagagt gtcggtgcca tcggcggatt tggcgcgggc aatcatccgc
     1141 tgttcgaata tctgcgctat gccatgcgcc cttacatgtt cacggcgtca tcttcgccgg
     1201 cctcgatcgc gacttcgatt gcggccatcc gcaagcttgc cgccgaaccg cagcggcgcg
     1261 acaggctgcg cgaaaactcg gcacggctgt tccacggctt caaggcgctg ggtctcgaca
     1321 tgggctgcga cacggtcagt ccggtcatcg ctgtgaagtg cccggacgag ccttcgacca
     1381 tcgccatgtg gaacgcgttg ctcaaggcgg gcgtctatgt gaacatcgcg ctgccgcctg
     1441 gaacgccgaa caagctctgc ctgctgcgct gttccgtttc cgccgctcac accgatgctg
     1501 acatcgaccg gatcatcacg ctcttcgaca gcgtggtggc gcaggggcac ggcaagaagg
     1561 tggtgaacgg ctagcgcacg gccttgctgg aaagcagttt cggcagcaac atcagtccgg
     1621 cgatcaccgg gtggcgccgc tgccagggcg atatcgcgat cttctggccg gagtggcgtt
     1681 cgatcttcca gaccagatag tcggcgccgc cggaaaaggt gaaggatgcc ttgatcagtc
     1741 gtgcggcggc ccagagcttt cccgatgctc tcttccagcc ccagcttgcg ggttgcgctg
     1801 ccgttgcggc gagtgccgcg gccagatcgc gatagtagct ttcgtttgcc gccaccagct
     1861 gatctgcgcg gtcgcctgct tcggcccgga gttccgttgc gtaggtggcg cggaaaccgg
     1921 cggcccagat atccaacgga tcgcccatgg gagaaagggc cctggcatgc gcaaacatgg
     1981 tctgcgcggc tctggcggcg gccgaaatca cgtcgcggcg cgcctccgcg tcggcggcaa
     2041 agaccagcgc cgatggctgt gcgaagcggg cccagaaata gggattgctc cgcgtcatcc
     2101 cgacagcgaa gagcggcatc ggcaggacgg catacttggc gcgatagcgc tgtcccccga
     2161 tgtcgcattc cgcgtagtag acattgggcg gaatcagcct gcagcccagt cggctcagga
     2221 cattggccga gaccccgcgg agatcgcggg tcagcacata gagatcgatc agcgagtcgg
     2281 tcgtggcgac gccgcgcagg caggaaccat atgccaatac ggccatgacg ccgtcatggg
     2341 cgctgcgcac gtggctggcc atcgccgcga tctcgggtgg aacaggctgt tcgagcgacc
     2401 ttgccagcaa tgtctcggcg gcgttcattc accggcaaac ataggtgaag acggggccgg
     2461 cttcgagacg caggggttca cccaccggcg gctcgaagaa ctcgccatcg atgacgaagg
     2521 tcaccggcgt ggtcacctcc agcgccgggg tgcagaagct caccgcgccc tgcgggaact
     2581 tgcggtcctc gccgccatac atcatgggca ggagccagcg ggggatggag ggtacgggat
     2641 acggaaaggt ggtcacgcgg atcgggccgg tcttgccgcc ccagaaaggc cgcgtgttga
     2701 gcaccagctt ctccagcgtg gtggccagca tcagcagctg attgccatcg gcataacgat
     2761 ggccctcggc ttcgacacgc accggatagg gccggtcgaa acgggtcgta tcggtgggat
     2821 cgggtttggt gaagagcgaa cggatcaccg agccggtgag ggtcgcaaag actccgtagc
     2881 cgccctttac gcccttggca ttgaacgcga tctgggtgaa gcgcgtggca tcagccaccg
     2941 ctccgcagcc cacgaacatg ccatggcgcg gactgccatc gcgcggattc acgcaacgca
     3001 ctgtcgggcg ggcgcgcagg tccgtgtgct tcagttcacg gatgaagtcg gcctgggccg
     3061 ccaatgacct gtgatgaaaa ccgaggtcgg ctgccgtcat gttggtggtg ccatgcggca
     3121 gcagggcgag gcgcggcaag cgggggaagg gcctgcgctc ggccaggatg gtctggatcg
     3181 cctggatcgt gccgtcgccg gaactgatga agaggtcgct tacgccctcg gtggcaagct
     3241 cgtcgacgag gccttcgatc atgtcgaagc ggtcgatgat cttgatcgag acattgccgg
     3301 tctgacgcag catgccggcc agcgccagac cct
//
LOCUS       DBNGVR010000403         3008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3486587, whole genome shotgun sequence.
ACCESSION   DBNGVR010000403 DBNGVR010000000
VERSION     DBNGVR010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3486587"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16A0_08560"
     CDS             complement(<1..347)
                     /locus_tag="AC16A0_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105426.1"
                     /translation="MTTRRNFIRFGALLLTTTAVASLPAALGQSGAALAAPKVSNLAV
                     QAAKAAFAGDYVSAGSLAQRSDDPAAIKLVELIYLRDKPNEAGYARIMAFLDAAPNWP
                     LSESLLKRAERSL"
     gene            537..1418
                     /gene="dapA"
                     /locus_tag="AC16A0_08565"
     CDS             537..1418
                     /gene="dapA"
                     /locus_tag="AC16A0_08565"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3105427.1"
                     /translation="MTFRGSFPALITPMKDGKVDEQAFRKFVNWQISEGSHGLVPVGT
                     TGESPTVTPEEHKRLVEICVAEAKGRVPVIAGTGSNSTAEAIEYTIHAKQAGADAALV
                     VVPYYNKPTQDGLYAHFKAIADAVDIPIFVYNVPGRTVANISVETLARLSQDCENIVG
                     TKDASADLTRPSRQRLASGTGFIQLSGEDGTALAFNAHGGVGCISVTANVAPRLCAQF
                     QEATLNGDFATALQLQDRLMPLHHALFVETSPGPIKYAVSLLGHCTPEARLPMVPVAE
                     STRTAVRQAMVHAGLLN"
     gene            1420..1917
                     /gene="smpB"
                     /locus_tag="AC16A0_08570"
     CDS             1420..1917
                     /gene="smpB"
                     /locus_tag="AC16A0_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3105428.1"
                     /translation="MSSKSGGAKTSRREEGIKVISDNRRARYDYEILQTFEAGVELKG
                     SEVKSLRTGHTNLAEAYAAMKGGELFLINSNIPEYREANRFNHEPKRPRKLLLHKKEI
                     DKLANGVQREGLTVIPLKMFFNRRGRVKVDLALARGRKLHDKRDAIKERSWDRERARL
                     MREKG"
     gene            1929..2438
                     /locus_tag="AC16A0_08575"
     CDS             1929..2438
                     /locus_tag="AC16A0_08575"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3105429.1"
                     /translation="MQHLVGRRLPDVDLEASSGSPVNPAKILGPSVLFCYPYTGRPGH
                     ADPPHWDHIPGAHGSTPQILAYSKNYGDFRRLGVKLFGVSLLDTEWQRDFVKRNALTF
                     RLLSDSERGFSGRLGLPLFETGGVDYLQRLTVVAYDGIIRVLRYPVPEPEQDAIVVLD
                     ICQALVASG"
     gene            complement(2430..>3008)
                     /locus_tag="AC16A0_08580"
     CDS             complement(2430..>3008)
                     /locus_tag="AC16A0_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3105430.1"
                     /translation="YRRAQRALHPDWFNAPVPSFGDAGAQLLIVGLAPGVQGANRTGR
                     PFTGDYAGELLYGTLIKFGFARGTFRADPGDGLELVSTLITNAVRCVPPQNKPTPLEI
                     RTCNAFLAAQIEGLSKLRAIVALGRIAHDATLTALGHRKALAPFGHGAEHAIGSLRLF
                     DSYHCSRYNTNTGVLTPAMFEAVFAKVRDYLA"
BASE COUNT          544 a          967 c          991 g          506 t
ORIGIN      
        1 tagagcgagc gctcggcccg cttgagcagc gattcgctca gcggccagtt gggcgccgcg
       61 tcgaggaacg ccatgatgcg cgcgtatccc gcctcgttcg gcttgtcgcg caggtagatg
      121 agttcgacca gcttgattgc agccggatcg tcggaccgct gtgccaggct cccggccgag
      181 acgtagtcgc cggcaaaggc cgccttggcc gcctggaccg ccaggttcga aaccttgggt
      241 gcagcaagcg cagccccgct ctgaccgagg gcggccggaa ggctcgcgac ggcggttgtg
      301 gtgagcagca aagcgccgaa gcgtatgaaa ttgcgacgtg tcgtcatctg ttaccaatac
      361 tcctaaccac cccgcctagg gaccaattat gcccttcctg tggcccgctg cggggccgga
      421 agcgcttgcc ggttgggggt cgaacgtcta tgttgccgcg ccggccagaa gggcgcggtg
      481 ccgaaccgac aggttagttc ggccatggtt tccaagtcat taccggagca aagacagtga
      541 cgtttcgtgg ttcgtttcct gcgctgatca cccccatgaa ggacggcaag gtggacgagc
      601 aggcgttccg caaattcgtc aactggcaga tttccgaagg cagccatggg cttgtaccgg
      661 tcggcacgac cggcgagagc cccaccgtca cgcccgagga acacaagcgc ctggtggaga
      721 tctgcgtggc cgaagcgaag ggccgggtgc cggtcatcgc cggaaccggc tcgaacagca
      781 cggccgaggc gatcgagtat accatccacg ccaagcaggc cggggccgat gcggcgcttg
      841 ttgtcgttcc ctattacaac aagccgacgc aggatggcct ctatgcccat ttcaaggcca
      901 ttgccgatgc ggtcgatatc ccgatcttcg tctacaacgt gccgggccgg acggtggcca
      961 acatctcggt cgagacgctg gccaggctgt cgcaggattg cgagaacatc gtcggcacca
     1021 aggacgcctc cgccgacctg acgcggccat cccgccagcg cctggcatcg ggaaccggct
     1081 tcatccagct ctcgggcgag gacggcacgg cgcttgcctt caatgcccat ggcggggtcg
     1141 gctgcatctc ggtgacggcc aatgttgcgc cgcgcctctg cgcgcagttc caggaggcaa
     1201 ccctgaatgg cgacttcgcc acggcgctgc agctgcagga ccggctgatg ccgctgcatc
     1261 acgcgctctt cgtcgagacc agcccgggcc cgatcaagta tgcggtaagc ctgctggggc
     1321 actgcacccc ggaagcgcgt ctgccgatgg tacccgtagc cgagtccacc cggacggcgg
     1381 tgcgccaggc catggttcat gcggggctgt tgaactgaga tgtcctccaa gagcggcggc
     1441 gcaaagacgt cgcgccggga agagggcatc aaggtcatct cggacaaccg ccgggcgcgc
     1501 tacgactatg agatcctgca gaccttcgag gccggcgtcg agctgaaggg ctccgaggtc
     1561 aaatccctgc gcaccggcca tacaaacctg gccgaggcct atgcggcgat gaagggcggc
     1621 gagcttttcc tcatcaactc caacatcccg gagtatcgcg aggccaaccg cttcaaccac
     1681 gagcccaagc gcccgcgcaa gctgctgctc cacaagaagg agatcgacaa gctggccaac
     1741 ggcgtgcagc gcgagggcct gaccgtaatt ccgctcaaga tgttcttcaa ccggcgcggg
     1801 cgggtgaagg tcgatctggc gctcgcccgc ggccgcaagc tgcacgacaa gcgcgacgcc
     1861 atcaaggaac ggtcctggga ccgcgaacgg gcgcgcctga tgcgggagaa gggctgagca
     1921 tgtttcacat gcagcacctc gtcggccgcc gcctgccgga tgtcgatctg gaagcctcct
     1981 cgggcagccc ggtcaatccg gccaagatcc tcgggccttc ggtgctcttc tgctatccct
     2041 acacgggacg gcccggccat gccgatccgc cgcattggga ccacatcccc ggcgcccatg
     2101 gatcgactcc gcaaatcctc gcttattcaa agaattacgg cgacttccgc aggcttggtg
     2161 tgaagctctt cggcgtgagc ctgctggaca ccgagtggca gcgcgatttc gtcaagcgca
     2221 atgcgctcac cttccgcctg ctgtcggact cggagcgggg cttctccggc cgcctgggcc
     2281 tgccgctgtt cgagaccggg ggcgtggact atctgcagcg cctgaccgtc gtggcctatg
     2341 acgggatcat ccgcgtgctg cgctacccgg tacctgagcc ggagcaggat gcaatcgtcg
     2401 tcctcgacat ctgccaggca ctggtggcgt caggctagat agtcccgcac tttggcgaag
     2461 accgcttcga acatggctgg cgtcaggacg ccggtgttgg tgttgtagcg cgagcagtga
     2521 tagctgtcga aaaggcgcag cgatccgatc gcgtgttcgg cgccatggcc gaagggggcg
     2581 agggccttgc gatggccgag cgccgtcagc gtggcatcat gggcgatacg gcccagcgcc
     2641 acgatggccc gcaattttga gaggccttcg atctgcgcgg ccagaaacgc gttgcaggtc
     2701 ctgatctcca gaggcgtcgg cttgttctgc gggggaacgc agcgcacggc gttggtgatc
     2761 agtgtactga cgagttcaag cccgtcgccg ggatcggcgc gaaacgtgcc cctggcaaaa
     2821 ccaaacttga tcagcgtgcc gtagagcagc tcaccggcat agtcgccggt gaagggacgc
     2881 ccggtgcgat tggcgccctg gacgcccggc gcaagtccca cgatcagcag ttgggcaccg
     2941 gcatcgccga aagacggcac cggcgcattg aaccagtcgg gatgcagcgc ccgctgcgcg
     3001 cggcggta
//
LOCUS       DBNGVR010000404         2449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3093011, whole genome shotgun sequence.
ACCESSION   DBNGVR010000404 DBNGVR010000000
VERSION     DBNGVR010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3093011"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC16A0_08585"
     CDS             complement(<1..464)
                     /locus_tag="AC16A0_08585"
                     /inference="COORDINATES: protein motif:HMM:NF018597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HIR3105431.1"
                     /translation="MKRSSAVFLAALAATVLALALAAQLRLVPDRYNPLAPLDLAEAP
                     NLMTGTKLWLMGDDPRACAAALRSAGVTLTELPEQTDRPGCVRRGTITLSKLHGARIR
                     PEEMRCDIALRLYLLDRHDLQPLAGQHLGSGVAEIAHFGSYSCRNIRGRSRL"
     gene            complement(504..935)
                     /gene="trxC"
                     /locus_tag="AC16A0_08590"
     CDS             complement(504..935)
                     /gene="trxC"
                     /locus_tag="AC16A0_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HIR3105432.1"
                     /translation="MSEIVCPSCTAVNRIPPDRLATEAKCGKCHKPLFDGHPLALTGQ
                     TFGRHLTRNSIPLLIDFWAEWCGPCKQMAPEFEKAARALEPHVRFAKLDTEAAPNEAA
                     AHGIRSIPTMILFQNGREAARQSGAMPAGHIIQWVREQIGA"
     gene            1099..1497
                     /gene="sdhC"
                     /locus_tag="AC16A0_08595"
     CDS             1099..1497
                     /gene="sdhC"
                     /locus_tag="AC16A0_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR3105433.1"
                     /translation="MTKTEHARRPLSPHLQIYRPMLTMMMSIFHRATGMATAVGVVLL
                     VWWLSAAATSDAYFDMVQRFFGHWFGRIVLFGFTWALVHHALGGLRHLLWDTGRGFEL
                     PVVELMARATIIGSVAVTILLWIIGYGMMP"
     gene            1494..1874
                     /gene="sdhD"
                     /locus_tag="AC16A0_08600"
     CDS             1494..1874
                     /gene="sdhD"
                     /locus_tag="AC16A0_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIR3105434.1"
                     /translation="MSMRTPLGKVRGLGSAKSGTEHWWLQRVTAIANLPLVIFLVIFV
                     IRHLGASRAEIVASIANPFVAILLALTFVSVLWHMRLGLQVVIEDYVHGHVAKFAALL
                     ANSFVTVLLGVAALYAILKMSFGL"
     gene            1874..>2449
                     /locus_tag="AC16A0_08605"
     CDS             1874..>2449
                     /locus_tag="AC16A0_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3105435.1"
                     /translation="MAAADAKKLAPKSVAINGRAYPITDHSFDVVVVGAGGAGLRAAL
                     GASQSGLKTACISKVFPTRSHTVAAQGGIAASLANMGADDWRWHMYDTVKGADWLGDQ
                     DAIEYLCRNAPTAVYELEHFGMPFSRTEDGRIYQRPFGGHMMNFGEGPPVQRTCAAAD
                     RTGHAMLHTLYGQSLRYSTEFFIEYFALDLIM"
BASE COUNT          407 a          775 c          804 g          463 t
ORIGIN      
        1 ctcagcctgc tccgcccgcg gatgttgcgg caggagtaac tgccgaaatg agcgatctcc
       61 gcaacgccgc tgccgagatg ctgccccgcg agcggctgaa ggtcgtgacg gtcgagaaga
      121 tagagccgca gcgcgatgtc gcagcgcatc tcctccggcc ggatccgggc gccgtggagc
      181 ttcgagaggg tgatggtgcc gcgtcggacg cagcctggcc ggtccgtctg ttccggcaat
      241 tctgtgagcg tcacgccggc actgcgcaag gccgcggcgc aggcccgggg atcgtccccc
      301 atcagccaga gcttggtccc ggtcatcagg ttcggcgctt cagcgagatc gagcggcgcc
      361 agcggattgt agcggtcagg aacgagacgg agctgcgcgg caagcgccag ggccagtact
      421 gtggcggcca gggccgcaag aaagactgcc gaactgcgtt tcactggcgc agatcagccc
      481 tgatttgcgc ggacaataag gcgtcaggca ccgatctgtt cgcgcaccca ctgaatgatg
      541 tggccggcgg gcatggcgcc cgactggcgg gccgcctccc ggccgttctg gaagaggatc
      601 atggtgggga tggagcggat gccatgggcc gccgcttcgt tgggcgcggc ctcggtatcg
      661 agcttggcga accgcacatg cggctccagc gcccgcgccg ccttctcgaa ctcgggggcc
      721 atctgcttgc acggaccgca ccattcggcc cagaaatcga tcaggagggg gatgctgttg
      781 cgggtgaggt gacggccgaa ggtctggccg gtcagcgcca ggggatggcc gtcgaacagc
      841 ggcttgtggc atttgccgca ttttgcctca gtggcaagac ggtcaggtgg tatccggttc
      901 acggcggtgc atgatggaca gacgatctca ctcatgacgt gcaattctcc aagggattct
      961 cagcatatgc aggcctgcat cacgagcaac catgatctgc gtcaagagcg ccggcaacaa
     1021 ccgatctatg tctttcggcg tatttgcgat attgcgctgc acaagctaaa accgtccatg
     1081 gggaacggga actgaccaat gacgaagact gaacatgcaa ggcggccgct ttcgccgcat
     1141 ctgcagattt accgcccgat gctgaccatg atgatgtcga tcttccaccg ggccacggga
     1201 atggcgacgg cggtgggcgt ggtgctgctg gtctggtggc tttcggccgc ggcgacctcc
     1261 gatgcctatt tcgacatggt gcagcggttc ttcggccact ggtttggccg catcgttctc
     1321 tttggattca cctgggcgct tgtgcatcac gcgctgggcg gactgcgcca tctcctgtgg
     1381 gacaccggcc gcggtttcga attgccggtg gtcgagctga tggcgcgcgc cacgatcatc
     1441 gggtcggtgg ccgtcaccat cctgctgtgg atcatcggct atgggatgat gccatgagca
     1501 tgcggacccc acttggcaag gtgcgcggcc tgggctcggc caagtccgga accgaacact
     1561 ggtggctgca gcgcgtcacc gccattgcca acctcccgct ggtgatcttc ctggtgatct
     1621 tcgtgatccg ccacctgggc gccagccggg ccgagatcgt cgccagcatc gccaatccct
     1681 tcgtggcgat cctgctcgcg ctcaccttcg tgtcggtgct gtggcacatg cggcttgggc
     1741 tgcaggtggt gatcgaggac tatgtccacg gccatgtggc caagttcgcg gccctgcttg
     1801 ccaacagctt cgtcaccgtg ctgcttggcg ttgccgcgct ttatgcaatt ctcaagatga
     1861 gcttcggact ttgatggcag ccgcagacgc aaagaaactg gcgcccaaga gcgtcgcgat
     1921 caacggccgc gcctatccga tcaccgacca cagcttcgac gtggtggtgg tgggtgccgg
     1981 cggcgcgggg ctgcgcgcgg ccctcggcgc ttcgcagtcg ggcctgaaga cggcctgcat
     2041 ctcgaaggtc ttccccacac gttcgcacac ggttgccgcc cagggcggca tcgccgcctc
     2101 gctcgccaac atgggcgccg acgactggcg ctggcacatg tacgacacgg tcaagggcgc
     2161 cgactggctg ggcgaccagg acgccatcga atatctctgc cgcaacgcgc cgaccgccgt
     2221 ctacgagctg gaacatttcg gcatgccctt ctcgcgcacc gaggacgggc gcatctacca
     2281 gcggcccttc ggcggccaca tgatgaattt tggcgagggc ccgccggtgc agcgcacctg
     2341 tgcagccgcc gaccgcaccg gccatgccat gcttcacacg ctctatggcc agagcctgcg
     2401 ttattcgacc gagttcttca tcgagtattt cgcgctcgat ctcatcatg
//
LOCUS       DBNGVR010000405         2985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1719244, whole genome shotgun sequence.
ACCESSION   DBNGVR010000405 DBNGVR010000000
VERSION     DBNGVR010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1719244"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /gene="ppk2"
                     /locus_tag="AC16A0_08610"
     CDS             <1..894
                     /gene="ppk2"
                     /locus_tag="AC16A0_08610"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR3105436.1"
                     /translation="PGRIERETPQRMRNDPEAIRHAFETGEFPYRTRLGTKVYEQQMA
                     ELQVELLKVQNWVKAAGERIIVIFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPTD
                     RERTQWYFQRYIENLPSGGELVFLDRSWYNRAGVERVMGFCTPNDYLEFMRQCPDLER
                     MFVRSGIRLFKYWFSVTREEQKHRFESRDKDPLKQWKLSPIDKASLDKWDDYTEAKEA
                     MFFYTDTADAPWIIIKSDDKKRARLACMQHFLSSLPYPNKNRKVVTGPDPLIVGSTAH
                     VIGRDEHILGKSLRPGNNKRR"
     gene            complement(915..2207)
                     /locus_tag="AC16A0_08615"
     CDS             complement(915..2207)
                     /locus_tag="AC16A0_08615"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3105437.1"
                     /translation="MANVAVVGAQWGDEGKGKLVDWLSERADVVVRFQGGHNAGHTLV
                     IDGVTYKLSLLPSGIVRPGKLSVIGNGVVIDPWALAGEIDKLAQQGVAVSRENLRIAG
                     NATLSLSVHRELDQMREAAAGAGKIGTTGRGIGPAYEDKVGRRAIRVNDLHDLSTIGM
                     KIDRLLAHHNALRRGLGQEEVRAADVLAQLSEIAPRILPYSDAVWAFLDDARREGKRI
                     LFEGAQGILLDIDHGTYPYVTSSNTEAGQAAAGSGLGPSAIGYVLGIAKAYTTRVGSG
                     PFPTEQENDIGEYIGQRGREFGTVTGRKRRCGWFDSVLVRQAIKTGGIHGIALTKLDV
                     LDGLEEIKVGVGYELDGKRIDRFPAAEEAQKRVVPIYESFEGWKGTTAGARSWAQLPA
                     QAIKYVKYLEELIECPVALLSTSPERDDTILMKDPFED"
     gene            complement(2300..>2985)
                     /locus_tag="AC16A0_08620"
     CDS             complement(2300..>2985)
                     /locus_tag="AC16A0_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HIR3105438.1"
                     /translation="PKQEARKVKQVEAEVAVAGFQALYAIPGRVSVDNTGTAKNVRID
                     TTVIEATLSARAVPKLDPTAYLTAAFTLPGETPLLPGTAMLYRDGVFMGQGHLPMLSP
                     GEEAKLGFGADDLIKVKRVEVRRQTGEEGLISTSNVDERAYDITVRSLHDFAIPVTIL
                     DQMPFSVQEEIKVTELPGMTVPSVRGFEKKRGVRAWTFDLEPDSEKVLKHGYKVTWPE
                     GFQVGMSLN"
BASE COUNT          554 a          979 c          902 g          550 t
ORIGIN      
        1 ccagggcgga ttgaacgcga aaccccacag cggatgcgga acgatcccga ggccatccgc
       61 cacgccttcg aaaccggcga gtttccctat cgcacgcgtc tcggcaccaa ggtctacgag
      121 cagcagatgg cggagctgca ggtggaactc ctcaaggtcc agaactgggt caaggccgct
      181 ggcgaacgca tcatcgtgat cttcgagggc cgggatgcgg cgggcaaggg cggcaccatc
      241 aagcgcttca tggaacatct caacccacgt ggcgcgcggg tggttgcgct cgaaaagccg
      301 accgaccgcg agaggaccca atggtacttt cagcgctaca tcgagaatct tccctcgggc
      361 ggcgaactcg tgttcctcga tcgctcctgg tacaatcgcg ccggcgtcga gcgggtcatg
      421 ggcttctgca cgcccaacga ctatctcgaa ttcatgcgcc agtgcccaga cctcgagcgc
      481 atgttcgtgc gctccggcat caggctcttc aaatactggt tctcggtcac ccgcgaagaa
      541 cagaagcacc gctttgagtc gcgcgacaag gacccgctga agcagtggaa gctgtcgccc
      601 atcgacaagg cctcgctgga caagtgggac gactataccg aagccaagga ggcgatgttc
      661 ttctacaccg acacggcgga tgcgccttgg atcatcatca agtccgacga taagaagcgg
      721 gccaggctgg cctgcatgca gcacttcctc tcgtcgcttc cctatcccaa caagaatcgc
      781 aaggtggtga ctggcccgga tccgctcatc gtgggatcga cggcccatgt catcgggcgt
      841 gacgaacata tcctcggcaa atcgctgcgg cccggcaaca acaagcggcg gtagcagatt
      901 ccggcgttcg cctgtcaatc ctcgaatgga tctttcatca ggatcgtgtc atcgcgctcg
      961 ggtgaggtcg aaagcagcgc caccgggcat tcgatcaact cttcaagata cttgacgtac
     1021 ttgatggcct gagccggcag ctgcgcccag gacctcgcac cggccgtggt gcccttccag
     1081 ccctcgaagc tttcatagat cggcacgacc cgcttctgcg cctcctcggc cgccgggaag
     1141 cggtcgatgc gtttgccgtc gagctcgtaa cccacgccga ccttgatttc ctcaaggccg
     1201 tcgagaacat cgagcttggt cagcgcaatg ccatgaatgc cgccggtctt gatcgcctga
     1261 cgcacgagaa ccgaatcgaa ccagccacag cgccgtttgc gccccgtcac cgtgccgaat
     1321 tcgcggccgc gctggccgat gtattcgccg atgtcgtttt cctgctcggt ggggaaagga
     1381 ccggagccga cccgggtggt gtaggccttg gcgatgccca gtacatagcc gatggccgaa
     1441 ggccccagac ctgaacctgc cgccgcctgc cctgcctccg tgttggatga ggtgacatag
     1501 ggataagtcc cgtggtcgat atcgagcagg atgccctggg cgccttcgaa caggatgcgc
     1561 ttgccttcgc gccgggcgtc atcgagaaat gcccagactg catcggaata gggcaggatg
     1621 cggggtgcga tctcggaaag ctgggccagc acatcggcag cgcgaacctc ctcctgaccg
     1681 agaccgcggc gcagcgcatt gtgatgggcc agcagccggt cgatcttcat gccgatcgtc
     1741 gagaggtcat gcaggtcgtt gacccggatc gcccggcgcc cgaccttgtc ttcataggcc
     1801 ggcccgatac cgcggccggt cgtgccgatc ttgccagccc cggcggccgc ctcgcgcatc
     1861 tggtctagtt cacggtgaac cgaaagactc agggtggcat ttccggcgat ccgcaagttc
     1921 tcccggctaa cagccacgcc ctgctgggcc agcttgtcga tctctccggc aagggcccag
     1981 ggatcgatga ccacgccgtt gccgatgacc gagagcttgc cagggcgcac gatgccggag
     2041 ggcagcagcg acagcttgta ggtgacaccg tcgatcacca gcgtgtggcc ggcattgtgc
     2101 ccgccctgga aacgcaccac cacatcggca cgctccgaca gccaatccac cagcttgccc
     2161 ttgccttcgt cgccccactg ggcgccgacc acagcaacat tcgccatttc aggttccttt
     2221 gacacatgga aacggccccg ggcggaattc cgccggggcc gttgtcggct catacaccat
     2281 tgatggcggg aggcaaagcc tagttgagac tcattcccac ctggaaccct tccggccagg
     2341 tcaccttgta gccgtgtttc aggaccttct cggaatccgg ttccaggtcg aaggtccagg
     2401 ccctcacgcc ccgtttcttc tcgaagcccc ttaccgacgg taccgtcatg cccggcagct
     2461 cggtgacctt gatctcttcc tgcacggaga agggcatctg atcgaggatg gtgaccggaa
     2521 tcgcgaagtc atggaggctc ctgacggtga tgtcataggc gcgctcatcc acgttgctgg
     2581 tggagatgag cccctcctct ccggtctgcc gcctgacctc cacgcgtttc accttgatga
     2641 gatcgtcggc accgaagccg agcttcgcct cttcgcccgg cgacagcatg ggcaggtggc
     2701 cctgccccat gaagactcca tcgcgataga gcatggcggt gccgggcagc agcggcgtct
     2761 cgccgggaag cgtgaaggcc gcggtgagat aggccgtggg gtcgagcttc ggaacggcgc
     2821 gcgcagacag ggttgcctcg atcaccgtcg tgtcgatgcg gacattcttc gccgtgcccg
     2881 tattgtccac cgagacgcgg cccggaatgg catagagtgc ctggaagccg gcgaccgcca
     2941 cctcggcctc cacctgcttc actttcctgg cttcctgctt cggcg
//
LOCUS       DBNGVR010000406         2912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_540682, whole genome shotgun sequence.
ACCESSION   DBNGVR010000406 DBNGVR010000000
VERSION     DBNGVR010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_540682"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1284)
                     /locus_tag="AC16A0_08625"
     CDS             complement(40..1284)
                     /locus_tag="AC16A0_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HIR3105439.1"
                     /translation="MLSDFAAAQARRHFPALALADAGRPRVYFDNPAGTQVPQQVVDR
                     TVDCLVNRNANLGGYFATTMAAGELVDEAHRACADFYNAATPAEIVFGQNMTTLTFHM
                     SRCLGRKFRKGDEIVLSRMDHDANVAPWLLLAEDLGLTVRWMDFDTGTYEFPDDALTR
                     VLSDRTRLVAMGMASNCTGTVNDTKRFAAQARAAGAIAYLDAVQFAPHYAIDVQELGA
                     DIVVSSAYKWFGPHQGVLWGRKDLLAETFGYKVRPAGEDLPHKFETGTLSHEGMAGCL
                     GAIEYLEQFGTGETRAKRISSAWTAMAAYEQKLTLKLIEGLRSFKGLTVRGITSANAM
                     HRRVPTVSFTLDGHHPQALAKAFAADNIFVWSGHNYAIEPVTRMGLMDKGGVLRVGLA
                     HYNTEAEVDALLSSLARQIKSN"
     gene            1442..2143
                     /locus_tag="AC16A0_08630"
     CDS             1442..2143
                     /locus_tag="AC16A0_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR3105440.1"
                     /translation="MSDEGELDLNSLNDEELVQQVHDDLYDGLKEEVEAAVHILLGRG
                     WTPYKVLTEALVEGMRIVGVDFRDGILFVPEVLLAANSMKAGMFILRPLLIATGAPRQ
                     GKMVIGTVKGDIHDIGKNLVGMMMEGAGFEVIDLGINNPVEKYLAAMEEHKPDILGMS
                     ALLTTTMPYMKVVIDTMKEKGMRDDYIVLVGGAPLNEEFAKAVGADAYCRDAAVAVET
                     AKSFMARKHNQMAAG"
     gene            2166..2801
                     /locus_tag="AC16A0_08635"
     CDS             2166..2801
                     /locus_tag="AC16A0_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR3105441.1"
                     /translation="MTIVNLQPSDEFEFKPGQGRVLLLACGALAREIVDLIEHNRWTG
                     FDLQCLPAKWHNTPEKIVPALREKIRAARGSYPSIFVLYGDCGTGGLLDRMLEEEGVE
                     RIDGPHCYAFFSGNEAFAAKADDDVTAFFLTDYLAKHFDKLIWAGLGLDRHPELLPLY
                     FGNYTRIVYLAQTRNEDIAKKAMAAAKKLGLAYEYRFTGYGELEADLGSRA"
BASE COUNT          521 a          925 c          948 g          518 t
ORIGIN      
        1 cgagaccgat gatggcgagc ttgaagggca tgggagacct cagttcgatt tgatctggcg
       61 ggccagcgag gagagcagtg cgtcgacctc ggcctcggtg ttgtagtgtg cgagcccgac
      121 gcgcagcacg ccgcccttgt ccatcagtcc catgcgggtc acgggctcga tggcgtagtt
      181 gtggccggac cagacgaaga tattgtcggc ggcaaaggcc ttggccaggg cctggggatg
      241 gtgaccgtcc agcgtgaagg acacggtggg cacgcggcgg tgcatggcgt tggccgaggt
      301 gatgccgcgg acggtgaggc ccttgaagct ccgcagtccc tcgatcagct tcagcgtcag
      361 cttctgttcg taggccgcca tggcggtcca ggcgctggat atccgctttg cgcgggtctc
      421 gccggttccg aactgctcga gatactcgat cgcgcccagg cagcccgcca tgccctcgtg
      481 cgagagcgtg ccggtctcga acttgtgagg caggtcctcg cccgctggcc gcaccttgta
      541 gccgaaggtt tccgccagca ggtccttgcg gccccagagc acgccctgat gcggaccgaa
      601 ccacttgtag gccgaggaga ccacgatgtc ggcgcccagc tcctgcacgt cgatggcata
      661 gtggggcgcg aactggacgg cgtcgagata ggcgatggca ccggccgccc tggcctgtgc
      721 cgcgaagcgt ttcgtgtcgt tgaccgtgcc ggtgcagttg gaggccatgc ccatggccac
      781 aagccgggtc cggtcgctca gcaccctggt cagggcatcg tcgggaaact cgtaggtgcc
      841 ggtgtcgaag tccatccagc gcaccgtcag gccaaggtcc tcggccagca gcagccaggg
      901 cgccacattg gcatcgtggt ccatgcgcga cagcacgatc tcgtcgccct ttcggaactt
      961 gcggcccagg cagcgcgaca tgtggaaggt gagcgtcgtc atgttctgcc cgaagacgat
     1021 ctccgcgggc gtggccgcat tgtagaaatc ggcacaggcc cggtgcgcct cgtccaccag
     1081 ctcgccggcc gccattgtgg tggcgaagta gccgcccaga ttggcattgc gattgaccag
     1141 gcagtccacc gtccggtcca ctacctgctg cgggacctgg gttccggccg ggttgtcgaa
     1201 atagacccgt ggccgccccg cgtcggcgag ggcaagggcg gggaagtgcc tgcgcgcctg
     1261 cgcggcggcg aaatcggaca gcatgccgga aaattcctca agcgttgaga atccttcacc
     1321 aatagccgtc cttggccctt gcctcaaccg tcgctttccg gcatgtggac gtcgcccaag
     1381 gggggtggcg gcgcaggcat tcccgctata aggggcgatc cgtttcttgc tggagactcc
     1441 gatgagcgat gaaggcgaac ttgacctgaa cagcctcaat gacgaggagt tggtgcagca
     1501 ggttcatgac gacctctatg acggcctcaa ggaagaggtc gaggcggccg tccatatcct
     1561 cctcggccgc ggctggacgc cctacaaggt cctgacggaa gcgctggtcg aaggcatgcg
     1621 catcgtgggc gtcgacttcc gtgacggcat cctcttcgtg ccggaagtgc tgctcgccgc
     1681 caactccatg aaggccggca tgttcatcct tcgcccgctc ctcatcgcca ctggcgcccc
     1741 gcgccagggc aagatggtga tcggcacggt gaagggcgac attcacgaca tcggcaagaa
     1801 cctcgtcggc atgatgatgg aaggtgcggg tttcgaagtc atcgacctcg gcatcaacaa
     1861 tccggtcgag aagtatcttg cggcgatgga agagcacaag cccgacatcc tcggtatgtc
     1921 ggcgctgctc actaccacca tgccctacat gaaggtcgtc atcgacacca tgaaggagaa
     1981 gggcatgcgc gacgattaca tcgtgctggt cggcggcgcg ccgctcaacg aggaattcgc
     2041 caaggccgtt ggcgccgacg cctattgccg cgatgcggcg gtggccgtcg agaccgccaa
     2101 gagcttcatg gcgcgcaagc acaaccagat ggcggcgggc tgaggcccaa cgcctccggg
     2161 tcggcatgac catcgtcaac ctgcagccgt ccgacgaatt cgaattcaag ccgggacagg
     2221 ggcgcgtgct gcttctggcc tgcggggccc tggcgcgcga gatcgtcgat cttatcgaac
     2281 acaatcgctg gaccggcttc gacctccagt gcctgccggc caagtggcac aacacgcccg
     2341 agaagatcgt tccggcgctg cgcgagaaga tccgcgccgc gcgcggcagc tatccctcga
     2401 tcttcgtgct ctatggcgac tgcggcaccg gcggcctgct cgatcggatg ctggaggaag
     2461 aaggcgtcga gcgcatcgac gggccgcact gctatgcctt cttcagcggc aacgaagcct
     2521 tcgcggcaaa ggccgatgat gatgtcacgg ccttcttcct cacggattat ctcgccaagc
     2581 atttcgacaa gctgatctgg gccggcctcg ggcttgaccg gcaccccgaa ctgctgccgc
     2641 tctattttgg aaactacacc aggattgtct acctggcgca gacccgcaac gaggacatcg
     2701 ccaagaaggc catggcggcc gcgaagaagc tcggccttgc ctatgaatac cgcttcaccg
     2761 gctatggcga gctggaagcc gatctcggat cgcgggccta gccttcattc atccatctgg
     2821 cgcggcggta ggttccgccg gcgatctcgt agtaatcccc cgccgactcg cagaagatgt
     2881 gaccttcgag caccagcccg ctcggcccgt cg
//
LOCUS       DBNGVR010000407         2744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5060454, whole genome shotgun sequence.
ACCESSION   DBNGVR010000407 DBNGVR010000000
VERSION     DBNGVR010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5060454"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="AC16A0_08640"
     CDS             complement(<1..576)
                     /locus_tag="AC16A0_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF993 family protein"
                     /protein_id="HIR3105442.1"
                     /translation="MHITLPDTRGSLETYALRGEALPMAKPKVPFNRVAFAAAHVVAD
                     PFGNADPSGPPAIDWGATLAYRRHLLDLGFGIAEAMDTSQRGMGLDWPNALELIDRSL
                     KDAGPRAGMIYSGCGTDHLVASAATSLDEVVRAYLEQLHAIQKLGGRIILMASRALVR
                     AARSPEDYIKVYERVLAEADHPVILHWLGDMF"
     gene            complement(579..1340)
                     /locus_tag="AC16A0_08645"
     CDS             complement(579..1340)
                     /locus_tag="AC16A0_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HIR3105443.1"
                     /translation="MTGSALVTGARRGIGKAIALALADAGFDVAVADVAASEELEAVA
                     EAIRGRGRKAAAIAGDIAAVETHAILLDAAEQALGPLTTLVNNAGVSVLSRGDLLDVT
                     PESYDRCDRVNARGTFFLTQAFARRLLGRAGDTHRSIIVISSANAAAPSIARGEYCIS
                     KAGASMISKLFAARLSDDGIGVYEIQPGFIETDMTAPSKAKYDKLIAEGLTVIRRFGR
                     PEEVARIAVTLATGLLPYTSGQIIQADGGLLSVRY"
     gene            complement(1337..2005)
                     /locus_tag="AC16A0_08650"
     CDS             complement(1337..2005)
                     /locus_tag="AC16A0_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3105444.1"
                     /translation="MVKSPSQRTRDPEATRARILAAAKAEFARKGLGGARVDDIAARA
                     KANKRMLYHYFGNKEDLFRITLEDAYADFRAAEARLEIEKDDPITAIKRLVSFTWKYY
                     LDNPEFITLVNSENLHKARHIRNSKRMREMSRPFVGRMRDLLARGAAAGLFRADLDPV
                     QVNITIAATGYYYLTNRFTGSIVFERDLMSEAALAQRVDFNTRTILRMVCTPATLARI
                     EAKA"
     gene            2093..>2744
                     /locus_tag="AC16A0_08655"
     CDS             2093..>2744
                     /locus_tag="AC16A0_08655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3105445.1"
                     /translation="MYDYVVVGGGSAGCVLAARLSEAPEARVLLIEAGPKDNDPYIHM
                     PVGFFKMTSGPLIWGYETAPLRHANNRRAVYPQARVLGGGSSINAEIYTRGCPEDYDN
                     WATLHDCEGWSWRDLQPCFIRSEGNARLGGADHGIDGPLAVSDLQSPNRVSLAYVQGC
                     MDIGMPHNPDFNSGRQEGAGLYQTTTRDGRRCSAAVGYLRPVMGRKNLQVRTDLHVT"
BASE COUNT          455 a          873 c          933 g          483 t
ORIGIN      
        1 gaacatgtcg ccgagccagt gcaggatgac cggatggtcc gcctcggcca gcacacgctc
       61 atagaccttg atgtagtcct cgggcgatcg ggcggcgcgc accagcgctc ggctggccat
      121 gaggatgatg cggccgccca gcttctggat ggcatggagc tgttcgagat aggcgcgcac
      181 cacctcatcg aggctggttg ccgcggacgc aaccaggtga tcggtgccac agccggagta
      241 gatcatgcca gcgcgcgggc cggcgtcctt gagcgagcgg tcaatgagtt cgagcgcatt
      301 gggccagtcg agccccatgc cccgctgcga cgtgtccatg gcttccgcga tgccgaagcc
      361 gagatcgagg agatggcggc gataggccag ggtggcgccc cagtcgatgg ctggcggccc
      421 cgacggatcg gcattgccga agggatccgc cacgacatgc gcggcggcga aggcgacccg
      481 gttgaacggc accttgggct tcgccatggg aagggcttcc ccccgcagcg cgtaggtctc
      541 gagcgaacct ctcgtatcgg gaagggtgat gtgcacgcct agtatctcac gctgagcagg
      601 ccgccatcgg cctggatgat ctgtccgctg gtatagggca gcaggcccgt ggccagggtg
      661 acggcaatgc gcgccacctc ctcgggcctg ccgaagcgcc ggatgaccgt gagcccttcg
      721 gcgatcagct tgtcgtactt cgccttcgac ggcgcggtca tgtcggtctc gatgaagccc
      781 ggctggatct cgtagacgcc gatgccgtca tcggaaaggc gcgcggcaaa gagcttcgag
      841 atcatcgagg cgccggcctt ggagatgcag tattcgccgc gggcgatcga cggtgctgcg
      901 gcattggccg aggaaatcac gatgatgctg cggtgcgtgt cgccggccct gccaagcagc
      961 cgccgggcga aggcctgggt caggaagaag gtgccgcggg cattgaccct gtcgcagcgg
     1021 tcgtagctct cgggcgtgac gtcgagaaga tcgccgcggc tgagcaccga gacgccggca
     1081 ttgttgacca gcgtggtgag cgggcccagg gcctgttcgg ccgcatccag caggatcgcg
     1141 tgcgtttcga ctgcggcgat gtcgccggcg atcgccgcgg ccttgcggcc cctgccgcga
     1201 atggcctccg ccacggcctc cagttcctcc gacgcggcga cgtcggccac ggccacatcg
     1261 aagcccgcat ccgccagcgc cagcgcgatg gccttgccga tgccccggcg ggcacccgta
     1321 acaagtgctg accccgtcat gccttggcct cgatcctggc gagcgtcgcc ggtgtgcaga
     1381 ccatcctgag aatggtccgg gtgttgaagt cgacgcgctg cgccagtgca gcctcgctca
     1441 tcaggtcgcg ttcgaagacg atcgatccgg tgaagcggtt ggtgaggtag tagtagcccg
     1501 tcgcggcgat ggtgatgttg acctggaccg ggtcgaggtc ggcgcggaac agccctgccg
     1561 cagcaccgcg cgcaagaaga tcgcgcatgc ggccgacgaa gggacgactc atctcgcgca
     1621 tgcgcttcga attcctgatg tggcgggcct tgtggagatt ctcgctgttg accagcgtga
     1681 tgaactccgg attgtcgaga tagtatttcc aggtaaaact gacgaggcgt ttgatcgccg
     1741 tgatcggatc gtccttctcg atctccagcc gggcttccgc cgcccggaaa tcggcatagg
     1801 catcctcgag cgtgatgcgg aacaggtctt ccttgttgcc gaagtagtga tagagcatgc
     1861 gtttgttggc cttggcccgc gcggcaatgt cgtcgacacg ggcgccgccc aggcccttgc
     1921 gggcaaactc ggccttcgcc gccgcaagaa tgcgtgcgcg cgtcgcttca gggtcgcgcg
     1981 tccgctggga tggcgatttg accacgatcc gccccttgaa ataaccagat ggttatttat
     2041 aagatggcgc gtctcgactg gcaagcccct cggagcgccg tgatgtatga cgatgtatga
     2101 ctatgtggtc gtgggtggcg gctccgctgg atgtgtcctg gcggcccggc tgtccgaggc
     2161 gccggaggcc cgtgtcctgc tgatcgaggc cggcccgaag gacaacgacc cctacatcca
     2221 tatgcctgtc ggcttcttca agatgacctc cggacccctg atctggggtt atgaaaccgc
     2281 gcccctgcgc catgccaaca accgccgggc cgtctatccc caggcgcggg tgctgggcgg
     2341 cggcagctcg atcaatgccg agatctacac gcgcggctgc ccggaggact acgacaactg
     2401 ggccacgctg catgattgcg agggctggag ctggcgcgac cttcagccct gcttcattcg
     2461 ctccgagggc aatgcgcggc tgggcggcgc cgaccacggc atcgatggtc ccctcgctgt
     2521 ttcggatctg cagagcccca atcgcgtctc gctggcctat gtgcagggct gcatggacat
     2581 cggcatgcca cacaatccgg acttcaactc cgggaggcag gaaggcgcgg gcctctatca
     2641 gaccaccacg cgcgatggcc gccgctgcag cgccgcggtg ggttacctgc gcccggtcat
     2701 ggggcggaag aacctgcagg tccgcaccga tctacacgtc accc
//
LOCUS       DBNGVR010000408         2407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4667554, whole genome shotgun sequence.
ACCESSION   DBNGVR010000408 DBNGVR010000000
VERSION     DBNGVR010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4667554"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="AC16A0_08660"
     CDS             <1..465
                     /locus_tag="AC16A0_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3105446.1"
                     /translation="DLHSADWTADDAYELAPAARRFENWECYYAGKLGLAVAMRYARS
                     IGMDRIEARVLALGAVLRRELAACPGVTLTDTGEMRCGIVTFVAAGCAAAEVHARLRQ
                     QAINVSVSSGERARFDLLRRGYRDVVRASVHYYNTEDEILRFCAAIRSLNGN"
     gene            532..1437
                     /locus_tag="AC16A0_08665"
     CDS             532..1437
                     /locus_tag="AC16A0_08665"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3105447.1"
                     /translation="MHNIDPVMFTVTGLEVYWYGFAYSSGFAFMLAWMAWQRRLLGWS
                     SREVLLAGVIFAAFILIGARIFDIAVYEWDWYREKPAQIPQLWRGGMASHGVLIGALL
                     AAVLTAWLTATPVLMLIDSLVVAAAFIPGISRIGNFIEGGVIGTVTTLPWGVKFPDVE
                     GFRHPVALYDGAKNLLLVPLLMATLRRWPAGRGVATAVFLLGYGGLRFLVDQLRDYES
                     VLWGLGPGQWFNLAMAAAGAAMLAICLRRPAPALPARTPPATERIPVVLPIAMLVLLL
                     LPLSIPTSWTREYIAILRHAAPANP"
     gene            complement(1442..>2407)
                     /locus_tag="AC16A0_08670"
     CDS             complement(1442..>2407)
                     /locus_tag="AC16A0_08670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016218.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3105448.1"
                     /translation="AATEEKQLTQILVIGDALAGGLGAGLSRMADTETYVVTNRFNEE
                     SGLARPEVYDWAERLPKILESNSFDVIVVMLGTNDRQQIRDGNLRYAFGTPEWTQAYE
                     AQLDRLLNELAASGASIYWIGLPPMGDPEYDEAMTAISALQKARAEAKDVRFIDMRPF
                     FLSEDGRYTDMGKDDNGAMVKLRGRDGVSFFKTGNNRMGGIVLDAIAKAEPAALPEED
                     TSAGIAAEAAIVRAPLPQRAVPEFGQALLQGEPLTMRPRDISTTALLVLGRGSSMSPA
                     EASAALASITEPGSAAAALFTDGVILKPPAGRLDDFSVPPRPAAE"
BASE COUNT          359 a          813 c          785 g          450 t
ORIGIN      
        1 gatctccatt ccgccgactg gacggccgac gatgcctatg agctggcgcc cgctgcacgc
       61 cgcttcgaga actgggagtg ctactatgcg ggcaagctcg gccttgccgt ggcgatgcgt
      121 tatgcgcggt cgatcggcat ggatcgcatc gaagcccgcg ttcttgcatt gggcgcggtg
      181 ctgcggcgcg aactagctgc ctgccccggc gtgacgctga ccgatacggg cgagatgcgc
      241 tgcggcatcg tcaccttcgt cgcggcaggg tgcgccgcag cggaggtgca tgcgcggctg
      301 cgccagcagg cgatcaacgt ctccgtttcc tcgggcgagc gcgcccgctt cgacctcctg
      361 cgccgcggct atcgcgacgt ggtgcgcgcc agcgtgcatt actacaacac ggaggacgag
      421 atcctccgct tctgtgccgc catccgctcc ctcaacggaa attgaccctc atcaaggcat
      481 gccggggccg gagctgcgat caagctgcag gagggccagt ccttgggcta tgtgcacaac
      541 atcgatccgg tcatgttcac ggtcaccgga ctggaggtct actggtatgg ctttgcctat
      601 agctcgggct ttgccttcat gctcgcctgg atggcctggc agcgccggtt gctgggctgg
      661 tcgtcgcgcg aggtgcttct cgccggcgtg atcttcgcag ccttcattct catcggcgcg
      721 cgcatcttcg atatcgccgt ctacgaatgg gattggtacc gggagaaacc tgcccagatt
      781 ccgcagctct ggcgcggcgg catggccagc catggcgtgc tgatcggtgc gcttctggcc
      841 gccgtgctga cggcatggct gacagcaacg ccggtgctga tgctgatcga cagcctggtc
      901 gtcgcagcgg ccttcatccc cggcatcagc cgcatcggca acttcatcga aggcggggtg
      961 atcggaaccg tcacgacgct gccctggggc gtcaagttcc ccgatgtcga aggcttccgg
     1021 catccggtgg cgctctatga tggcgccaag aatctcctgc tcgtgcccct gctgatggcc
     1081 acgctgcggc gatggcccgc cgggcggggc gtcgccaccg cggtcttcct gctgggctac
     1141 gggggcctcc gtttcctcgt cgaccagttg cgcgactacg aatcggttct ctggggcctt
     1201 ggccccggac agtggttcaa tctcgccatg gccgcggcgg gggcagcgat gctggcgatc
     1261 tgcctgcgcc ggccggcacc ggcactgccc gctcgcactc caccggccac agagagaatt
     1321 cccgtcgtgc tgccgattgc catgctcgtg ctgctgctgt tgccgctgtc cattccgaca
     1381 agctggacgc gcgaatacat cgccatcctc cgccacgccg caccggcgaa cccttagggc
     1441 gtcactccgc cgcaggcctg ggcggaaccg agaaatcatc caggcggccc gccggcggct
     1501 tgaggatcac gccatcggtg aagagcgcgg cggcggcact tcccggttcg gtgatcgagg
     1561 ccagcgcggc cgaggcctcg gccggcgaca tggacgatcc ccggcccagc accagcaagg
     1621 cggtggtcga gatatcccgc ggccgcatgg tcagcggctc accctgcagc agggcctgcc
     1681 cgaactccgg cacggctcgc tgcggcaggg gcgcacgcac gatagccgcc tcggcggcaa
     1741 tcccggcgga ggtgtcctcc tccggcagcg cagcaggttc cgccttcgcg atggcatcca
     1801 gcacgatgcc gcccatgcgg ttgttgcccg tcttgaagaa gctgacgccg tcgcgcccgc
     1861 gcagtttcac catcgcgccg ttatcgtcct tgcccatgtc ggtgtagcgg ccatcctcgg
     1921 acaggaagaa cggacgcatg tcgatgaaac ggacgtcctt ggcttcggcc cgcgccttct
     1981 gcagggcact gatcgcggtc atggcctcgt catattcggg gtcgcccatg ggcggcagcc
     2041 cgatccagta gatgctcgcg cccgatgcgg ccagttcgtt cagcaggcgg tcgagctgcg
     2101 cctcataggc ctgtgtccac tccggggtgc cgaaagcgta acgcagattg ccatcgcgga
     2161 tctgctggcg gtcgttggtg cccagcatca cgacgatgac atcgaagctg ttgctttcga
     2221 gaatcttggg aagccgctcg gcccagtcat agacttccgg ccgcgccagg ccggattcct
     2281 cgttgaagcg attggtgacg acgtaggttt cggtatcggc catgcgcgaa agcccggccc
     2341 ccaacccgcc agccagggcg tcaccgatga ccaggatctg cgtcagctgc ttttcttccg
     2401 ttgcagc
//
LOCUS       DBNGVR010000409         5153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2114514, whole genome shotgun sequence.
ACCESSION   DBNGVR010000409 DBNGVR010000000
VERSION     DBNGVR010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2114514"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="AC16A0_08675"
     CDS             complement(<1..635)
                     /locus_tag="AC16A0_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105449.1"
                     /translation="MTSQPADLRVIRARRSFSENAWRTLKSAPFTAWLGMIIILVYAI
                     AALFAPWIAPYGESEIVGKQYLPPSAEFWFGTDQIGRDMFSRVIYGARNTIGIALATT
                     LLSFIIGGTLGVLTALKRGWLDQTISRIVDAFLAIPVLIFALMLLAVFGKSIMLLILI
                     LALLDSTRVFRLARATALNVVVMDYVESARLRGEGTGWIIFKEILPNILPP"
     gene            complement(632..1603)
                     /locus_tag="AC16A0_08680"
     CDS             complement(632..1603)
                     /locus_tag="AC16A0_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105450.1"
                     /translation="MHPVLRTVLQRLGLGLLTLFIVSIVIFMSLELLPGGFAEAILGQ
                     GATPETVAAFNREIGLDRPAYVRYVEWIAGVVRGDFGYSYAGLGGSIKRSVTEMIAPR
                     LYNTFFLAIMTAIFAVPLSLFLGVMAALYRNSFYDRTVNAVTLTTIAAPEFFIAYILM
                     FIFAVKLRWFYPTSTVSESTGFAEHVYRAALPAITLTLVIVAHMMRMTRAAIINLLAS
                     PYIEMAQLKGIPNREVIMKHALPNAWAPIATVIAFNLAYLVVGVVVVEVVYVYPGIGQ
                     LMVDSVSSRDMPVVQACALIFAATYILLNLSADIVSIITNPRLLHPK"
     gene            complement(1761..3368)
                     /locus_tag="AC16A0_08685"
     CDS             complement(1761..3368)
                     /locus_tag="AC16A0_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105451.1"
                     /translation="MTETEQKLKQAAKLAGRGKISRREFTQLALASGLTLAAANTMFT
                     TAARAEPKKGGTFKLGLAHGSTTDSMDPGLYYDQFTGTALWGTLSNSLTEIDAAGNIV
                     PDLAESFEPADGAKKWVFKLRKGVTFHNGKPVTADDVVASFMHHRGADSKSAAKSLLD
                     PVTSIKADGAETVIFELNSGNADFPYIASDYHIPILPRKEDGTADWESGNRTGPYTLD
                     KFEPGVSCTFNKNPNYFKSDKGWFDRVEFLAIKDVTARTNALNTGETQYLHRADLKTL
                     DLLKQNPNVAIFEKTGYGHYIYVMNVTAKPFDNQDVRNAIKYSLDREEILAKVFNGYG
                     SVGNDNPIAPTVKFAVDPQPKHVYDPEMAKSLLKKAGLESLAFDLSVADAAFTGAVDA
                     ALLWKEKAKACNIEINVIREPDDGYWDNVWLKKPFVASYWAGRPTCDWMFTTAYAADA
                     AWNDTFWKNPRFNELLVAARAETDEAKRAAMYAEMQQLVHTDGGLVNVVFNNFVDAHA
                     ANLGHGETAANWPMDGMKLTERWWFTS"
     gene            complement(3606..>5153)
                     /locus_tag="AC16A0_08690"
     CDS             complement(3606..>5153)
                     /locus_tag="AC16A0_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105452.1"
                     /translation="LGKLSRRDFMSFAIASGFTIAAANQMFVTAARAEAKKGGNFKAA
                     VGHGSTTDSLDPATWANGFTFGFGKSLFSAPLVQVNVKNEAAPHVAESFEPADGANKW
                     VFKIRKGITFHDGRTLTADDVVATINYHIGPDSKSPAKSVLSSITSVKADGPDTVIFE
                     LSGGNADFPFLLSDYHLGMYPSKDGKIEWEKGIGAGPFVLKSFEPGVRLAAERNPNYF
                     GTAWFDSVELLSMVDTAARTNAYLSGEVHFIDRADLKTIDLLKNAPETELYNVSGFAH
                     YTAPMHCDAAPFSDVNVRLAIKHAINRQELVDKILFGYGTPGNDNPISKAMKFAIDPQ
                     PVHTYDPDKAKSYLKKAGMENLKVDFSVSDAAFSGGVDAGLLIQESAKAAGIDINLIR
                     EPADGYWENVWLKKPWSLCYWGGRPVADMFMSVSLAAGAPWNDTRWNNARFNELLLAA
                     RAETDDAKRAAMYSEAQQLVHDDGGQIVLMFNNYVGALSTKVGHGEFNTDFDHDGGYM
                     WERWWMA"
BASE COUNT          978 a         1484 c         1706 g          985 t
ORIGIN      
        1 atcggcggca agatgttggg caggatttcc ttgaagatga tccagccggt gccctcgccg
       61 cgcagccttg cggactcgac atagtccatc accaccacgt tcaacgccgt ggcacgggcg
      121 agacggaaga cgcgggtgga gtcgagcagg gccaggatga ggatcagaag catgatgctc
      181 ttgccgaaga cagcaagcag catcagcgcg aagatcagca ccgggatggc gagaaaggca
      241 tcgacgatgc gcgaaatggt ctggtcaagc cagccgcgct tcagggcggt gagaacgccg
      301 agggtaccgc cgatgatgaa ggacaggagc gtggtcgcca gagcgatgcc gatggtgttg
      361 cgggcgccgt agatcacgcg cgagaacatg tcgcggccga tctggtcggt gccgaaccag
      421 aactcggcgg agggcggcag atattgtttg ccgacaatct ccgactcccc atagggcgcg
      481 atccagggcg cgaagagcgc ggcaatggca tagaccagaa tgatgatcat gccgagccag
      541 gcggtgaagg gcgcggactt gagcgtcctc caggcgttct cggagaagct gcgccttgcg
      601 cggatgacgc gaagatctgc gggttgactg gtcatttggg atgcagcaat cgcgggttgg
      661 tgatgatcga cacaatgtcg gcggagagat tgagcaggat gtaggtggcc gcgaagatca
      721 gggcgcaggc ctgcaccacc ggcatgtcac ggcttgatac ggagtcgacc atcaattgcc
      781 cgatgccggg gtagacatag accacttcga ccaccaccac gccaaccacc agataggcca
      841 ggttgaaggc gatgaccgtg gcgatcggcg cccaggcatt gggcaaagca tgtttcatga
      901 tgacttcgcg gttcggaatg cccttgagct gggccatctc gatgtaggga ctggccagca
      961 ggttgatgat cgccgcgcgc gtcatgcgca tcatgtgtgc gacgatgacg agggtcagcg
     1021 tgatggccgg cagcgcggca cgatagacat gttcggcaaa ccccgtcgac tcgctcaccg
     1081 tcgaggtggg gtagaaccag cgcagcttca ccgcgaagat gaacatcagg atataggcga
     1141 tgaagaattc gggtgcggcg atggtggtga gcgtgaccgc attcacggtg cggtcgtaga
     1201 agctgttgcg atagagggcc gccatgacac cgaggaacag ggacagcggc acggcgaaga
     1261 tggccgtcat gatggcgagg aagaaggtgt tgtagagccg gggcgcaatc atctcggtga
     1321 cgctgcgctt gatggacccg ccaaggccgg cataggaata gccgaagtcg ccgcgcacca
     1381 cgccagcgat ccactcgacg tagcggacat aggcggggcg gtcgaggccg atttccctgt
     1441 tgaaggcggc gacggtctcg ggagtcgcgc cctggccgag aatggcttcg gcaaagccgc
     1501 cgggcagcaa ttccagcgac atgaagatca cgattgaaac gatgaagagc gtgagcaaac
     1561 ccaggcccag acgctgcagc accgtccgca ggacaggatg catacattcc cccgatcggg
     1621 cctctcgacg tgcccttcag aattagacta gcagaacaac ccggcgacgc tagccaaaat
     1681 tcttcacctc ccgatgccaa aaaagaaccg gagtgcactg gcaccccggt tcaggacgtt
     1741 ctagctgagc gaacctcaac ttacgaggtg aaccaccagc gctccgtgag cttcatgccg
     1801 tccatcggcc agttggccgc ggtctcgcca tggccgaggt tggcggcatg ggcatcgacg
     1861 aagttgttga acaccacgtt caccaagccg ccatcggtgt gcaccagctg ctgcatttcg
     1921 gcatacatgg ccgcgcgctt ggcctcgtcg gtctcggcgc gggctgccac cagcagctcg
     1981 ttgaagcgcg ggttcttcca gaacgtgtcg ttccaggcgg catccgccgc ataggcggtg
     2041 gtgaacatcc agtcgcaggt gggacgtccg gcccagtagg aggccacgaa gggcttcttg
     2101 agccagacat tgtcccagta gccgtcgtcc ggctcgcgga tcacgttgat ctcaatgttg
     2161 caggccttgg ccttttcctt ccacagcagg gcggcatcga ccgcgcccgt gaaggcggca
     2221 tccgccaccg acaggtcgaa ggccagcgac tcgagcccgg ccttcttgag caggctcttg
     2281 gccatctcgg gatcgtagac gtgcttgggc tgaggatcga ccgcgaactt cacggtcgga
     2341 gcgatcggat tgtcgttacc gaccgagccg tagccgttga acaccttggc caggatctcc
     2401 tcgcggtcga gggagtactt gatggcgttg cgcacatcct ggttgtcgaa gggctttgcg
     2461 gtcacgttca tcacgtagat gtagtggcca tagcccgtct tctcgaagat ggcgacgttg
     2521 gggttctgct tgagaagatc aagcgtcttc aggtcggcac ggtgcaggta ctgggtttcg
     2581 ccggtgttga gtgcgttggt gcgggcggta acgtccttga tggcgaggaa ttccacccgg
     2641 tcgaaccagc ccttgtcgga cttgaagtag ttcgggttct tgttgaaggt gcagctgacg
     2701 cccggctcga acttgtcgag cgtgtagggg ccggtgcgat tgccggattc ccagtcagcc
     2761 gtcccgtctt cctttcgcgg aaggatcgga atgtgatagt cggacgcaat gtagggaaaa
     2821 tcggcgttgc cgctgttcag ttcaaagatc accgtctcgg cgccgtcggc cttaatcgat
     2881 gttaccgggt cgaggagcga cttggccgcc gatttggaat cagctccacg gtgatgcatg
     2941 aacgaggcaa cgacgtcgtc ggccgtcacc ggcttgccgt tgtggaacgt cacgcccttg
     3001 cgcagcttga agacccactt cttggcgcca tcggcaggct cgaagctttc ggcaagatcc
     3061 ggaacgatgt tgcctgcggc atcgatttcc gtcaggctgt tcgacagcgt gccccacagg
     3121 gccgtgccgg tgaactggtc gtagtatagc ccggggtcca tggaatcggt ggtcgagcca
     3181 tgtgcgaggc caagtttgaa ggtgccgccc ttcttgggtt cggcgcgtgc cgcagtggtg
     3241 aacatggtat tcgccgcggc cagggtgagg ccggaggcca gcgccagctg ggtgaactcg
     3301 cggcgcgaga tcttgccgcg gccagccagc ttcgcggcct gcttcagttt ctgttcggtc
     3361 tctgtcattt gcttccctct ctattgatgt cccgtgccgg tctcgaaatt cccaagaccc
     3421 aagtaaaatt catgggacgc ccgagttttt ctcgtggcca tgaactgcgc caaggccgcc
     3481 gcaatgcgtt ttgctccgct tgcggcacca taacgttgcg gtgagcctgt catgcgacga
     3541 gggccccgtc aatcgcttga cgaggccccc gaaatacaaa tttatgtaag gtcagcagac
     3601 tgccgttagg ccatccacca acgctcccac atgtaaccgc catcgtggtc gaagtcggtg
     3661 ttgaactcgc catgaccaac cttggtcgac agcgcgccga cgtagttgtt gaacatcagc
     3721 acgatctggc cgccgtcatc gtggaccagc tgctgggctt ccgaatacat ggccgcacgc
     3781 ttggcatcgt ccgtttccgc acgggccgcc agcagcagct cgttgaagcg ggcattgttc
     3841 cagcgcgtgt cgttccacgg cgcaccggct gccagcgaca ccgacatgaa catgtcggcc
     3901 accggacggc cgccccagta gcacaggctc cagggcttct tgagccagac attctcccag
     3961 tagccgtcgg ccggctcgcg gatcaggttg atgtcgatgc cagcggcctt ggcgctttcc
     4021 tggatcagca ggccggcgtc gacgccgccc gagaaggccg catccgagac cgagaagtcg
     4081 accttcaggt tctccatgcc cgccttcttg aggtagctct tggccttgtc cgggtcatag
     4141 gtgtggaccg gctgcgggtc gatggcgaac ttcatcgcct tcgagatcgg attgtcgttg
     4201 ccgggcgtgc cgtagccgaa gaggatcttg tcgaccagct cctggcggtt gatggcgtgc
     4261 ttgatggcca ggcgcacgtt cacgtcgctg aagggcgccg catcgcagtg catcggcgcg
     4321 gtgtagtgcg cgaagccgga cacattgtag agttcggttt ccggcgcgtt cttcagcagg
     4381 tcgatggtct tgagatcggc acggtcgatg aagtgcactt cgcccgagag ataggcgttg
     4441 gtgcgggcgg cggtgtcgac catcgagagc agctcgacgc tgtcgaacca cgcggttccg
     4501 aagtagttgg gattgcgctc ggccgcgagg cgcacgccgg gctcgaagga cttgagcacg
     4561 aagggaccgg cgccgatgcc cttttcccac tcgatcttgc cgtccttcga cgggtacatg
     4621 ccgagatggt agtccgacag gaggaagggg aagtcggcat tgccgccgct cagctcgaag
     4681 atcaccgtgt cggggccatc ggccttgacg ctggtgatgg aggacaggac cgacttggcc
     4741 ggcgacttgg aatccggacc gatgtggtag ttgatggtgg caaccacgtc gtcggccgtc
     4801 agcgtccggc cgtcatggaa agtgatgccc ttgcggatct tgaagaccca cttgttggca
     4861 ccgtcggccg gctcgaagct ttcggccaca tgcggggcgg cttcgttctt gacgttgacc
     4921 tgcaccagcg gcgcggagaa cagcgacttg ccgaagccga aggtgaagcc gttggcccag
     4981 gtggcgggat cgagcgagtc ggtggtcgac ccgtgaccga cggcggcctt gaagttaccg
     5041 cccttcttgg cttccgcgcg ggcggcggtg acgaacatct ggttcgccgc ggcgatcgtg
     5101 aagcccgagg cgatggcgaa ggacatgaag tcgcggcgcg acagcttgcc tag
//
LOCUS       DBNGVR010000410         4519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_740149, whole genome shotgun sequence.
ACCESSION   DBNGVR010000410 DBNGVR010000000
VERSION     DBNGVR010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_740149"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="AC16A0_08695"
     CDS             <1..1102
                     /locus_tag="AC16A0_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3105453.1"
                     /translation="IAAVTVLIIACPCALGLATPMSIMVGIGRGAQDGVLVKNAEALE
                     RMEKVDTLVIDKTGTLTEGRPAVTQVIAAEGMDANELLRLAASVEQASEHPLAAAIVA
                     EARHREILLARVLGFDSPTGKGAIGMVERKRMALGNARFLQELGISSAALEAQAELQR
                     RSGATVIFVAVNGQLAGMIAIADPVKPSTPAALAALRARGTGIVMLTGDNRTTAEAVG
                     RDLGITDIRAEVLPQDKADIVKALRAEGRVVAMAGDGVNDAPALAAADVGIAMGTGTD
                     VAIESAGITLLKGDLAGLVKAHNLSRATMCNIRQNLFFAFFYNAAGVPIAAGILYPFF
                     GILLSPIIGAAAMALSSVSVIGNALRLRTTRL"
     gene            complement(1097..1624)
                     /locus_tag="AC16A0_08700"
     CDS             complement(1097..1624)
                     /locus_tag="AC16A0_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3105454.1"
                     /translation="MTQTSRERTVFARNPLDMIMHQHAMQMQLCDMLERIADGLPDEV
                     DRRLCAQAASSLTYDLPLHHHDEETGLFPLLRERALPEDQIENILGRLATEHVTDTDF
                     AGEIAESLEILGRGDRVGNPDMVGYMLRGFFERYRRHVHWENTLVMPVARMRLMPEDL
                     EALTQQMERNRGDTL"
     gene            complement(1730..2551)
                     /locus_tag="AC16A0_08705"
     CDS             complement(1730..2551)
                     /locus_tag="AC16A0_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3105455.1"
                     /translation="MNAKKASSAKVHENRGTPLVEMKDISIAFGGIKAVDHASIDLYP
                     GEVVGLLGHNGAGKSTLIKILSGAYKRDHGEIFIEGKPVAINNPRDAKAQGIETIYQT
                     LALADNVDAAANLYLGRELRTKFGTLDDVAMEAECRKVMGRLNPNFRRFKEPVKALSG
                     GQRQSVAIARAIHFNARILIMDEPTAALGPQETAQVGELIKQLKAEGIGIFLISHDLH
                     DVFDLADRLTVMKNGRVVGTAHTSDVDHDEVLGMIIAGKCPPNAIPGPGALRSAA"
     gene            complement(2564..3910)
                     /locus_tag="AC16A0_08710"
     CDS             complement(2564..3910)
                     /locus_tag="AC16A0_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR3105456.1"
                     /translation="MTAVDNTQNPARSDEGAIARFLRATEIDARMLGMVAALLIIWIG
                     FDIYSGFLRDGEGLFGGSFLTPRNLWILLVQTSSIAVMTTGMVLIIVMRHIDLSVGSI
                     LGFTSVCIATMQVFWLGPMLGVGHPSIWIVTLILGLLLGGAIGAFHGTLIAYLGIPSF
                     IVTLGGLIVWRGAAWAVIRGETVAPMDKNFKLIGGGISGITPGSIGATASWILGAVIC
                     AAIIYAIYNNRVQRKRFNFPQRPMWAEYFLAITGCVLALGVTWLVNSYYWPKGVVDRY
                     VQATGIEVPPEGINIAFGYSVPVLIAMGVGLGMSFLATRTRFGRYVYATGGNPEAAEL
                     AGVNTKKLTVMIFALMGVLAAISASISSARLDSATNALGQFDELYVIAAAVIGGTSLA
                     GGLGTIYGAMLGALVMQSLQSGMTLLNFESATKDIVVGCVLVFAVFVDQVYRRRVK"
     gene            complement(4030..>4519)
                     /locus_tag="AC16A0_08715"
     CDS             complement(4030..>4519)
                     /locus_tag="AC16A0_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3105457.1"
                     /translation="ETFTDGWKPDAAQKNMEQCLTSVNNKVDAVISENDGMAGGVVAA
                     LEAQGLAGTVPVTGQDGDKAALNRVALGTQLVSVWKDARVLGKAAAEFANMIADGKAA
                     TELPGVGKFSGGAKGVEVNAVLIAPNPITRDNLNDVIDGGWVTKEEVCAGAPAGAVAA
                     CN"
BASE COUNT          788 a         1439 c         1492 g          800 t
ORIGIN      
        1 cattgccgcg gtgacggtgc tgatcatcgc ctgtccctgc gcactgggtc ttgcgacacc
       61 catgtcgatc atggtcggca tcggccgcgg agcccaggat ggcgtgctgg tcaagaatgc
      121 cgaagcgctc gagcggatgg aaaaagtcga tacgctggtc atcgacaaga ccggaaccct
      181 caccgaaggc cggccggcgg tgacgcaggt catcgccgcg gaaggcatgg acgcaaacga
      241 actgctgcgc ctggcggcca gcgtcgagca ggccagcgag catccgcttg ctgccgcgat
      301 cgtggcggag gcccggcacc gcgaaatcct gctggcccgt gtcttgggct tcgactcgcc
      361 gaccggcaag ggcgcgatcg gtatggtgga acgcaagcgg atggcgctgg gcaatgcaag
      421 gttcctgcag gaactgggca tctcctctgc tgctctggag gcgcaggccg agttgcagcg
      481 caggtccggt gccacggtga tcttcgtcgc ggtgaacggt cagctcgccg ggatgatcgc
      541 cattgccgat ccggtgaagc cgtcaacgcc agccgccctt gcggcgctgc gcgcgcgcgg
      601 caccgggatc gtcatgctga ccggcgacaa ccgtactacg gcagaggccg tcggccgcga
      661 tctcggcatc accgatatcc gcgccgaggt gcttccgcag gacaaggccg acatcgtgaa
      721 ggcgctccgg gccgaaggcc gtgtggtggc catggcaggc gacggcgtga acgacgcgcc
      781 ggcgcttgcc gcggcggacg tcggcatcgc catgggcacg ggcaccgatg tcgccatcga
      841 gagcgccggc atcacgctgc tgaagggcga cctcgcgggc ctggtgaagg cgcacaatct
      901 ctcgcgtgcg accatgtgca acatccggca gaatctgttc ttcgccttct tctacaatgc
      961 cgccggcgtc ccgattgcgg ccggcattct ctacccgttc ttcgggatcc ttctttcgcc
     1021 gatcatcggc gcggcggcca tggcgctgag ttcggtcagc gtgatcggca acgccctgcg
     1081 gttgcgcacg acgcgcctat agcgtgtcgc cgcggttgcg ctccatctgc tgggtgagtg
     1141 cctcaaggtc ctcgggcatc aggcgcatcc gggccaccgg catgaccagc gtgttttccc
     1201 agtgcacgtg gcggcggtag cgctcgaaga agccgcgcag catgtaacct accatgtccg
     1261 gattgcccac gcggtcgccg cggccaagga tttcgagcga ctcggcgatc tcgccggcaa
     1321 agtccgtgtc ggtgacatgc tcggtcgcaa ggcgcccgag aatgttctcg atctggtctt
     1381 cgggcagtgc cctctcgcgc aagaggggga acaggcccgt ctcttcgtca tgatgatgaa
     1441 gcggcagatc ataggtcagg ctggatgcgg cctgggcgca gagtctgcgg tcgacctcgt
     1501 ccggcagccc gtccgcgatc cgctccagca tgtcgcagag ctgcatctgc atggcgtgct
     1561 gatgcatgat catatcaagc gggttgcggg cgaacaccgt ccgctcgcgg gatgtctgtg
     1621 tcatgagtcc gccagaaacc aacgtgaagc cgcaatcatg cggttgcggt caatccgggc
     1681 tttgatccag atcaagcggc cgtcgaattc gggacggcgc aggatcgcat caggccgccg
     1741 atcgcagcgc gcccggccct ggaatggcat tgggcgggca cttgccggcg atgatcatgc
     1801 ccagcacctc atcgtggtcc acatccgatg tatgcgcggt cccgaccacc cggccattct
     1861 tcatcaccgt cagccggtcg gccaggtcga agacgtcatg caggtcatgg ctgatgagga
     1921 agataccgat gccctccgcc ttgagctgct tgatcagttc gccgacctgt gccgtttcct
     1981 ggggcccaag ggcggccgtc ggctcgtcca tgatcaggat gcgggcattg aagtggatgg
     2041 cccgggcgat ggcgaccgac tgccgctggc cgccggagag tgcctttacc ggttccttga
     2101 agcggcggaa gttgggattg aggcggccca tgacctttcg gcactccgct tccatcgcca
     2161 cgtcatccag cgtgccgaac ttggtgcgga gttcgcggcc aagatagagg tttgccgccg
     2221 cgtcgacgtt gtcggccagg gcaagggtct gatagatagt ctcgatgcct tgcgccttgg
     2281 cgtcgcgcgg attgttgatg gccaccggct tgccctcgat gaagatctcg ccatggtcgc
     2341 gcttgtaggc gccggagagg atcttgatca gcgtggactt gccggcgccg ttgtggccaa
     2401 gcaggcccac aacttcgccc ggatagagat cgatcgaggc atggtccacg gccttgatgc
     2461 cgccgaaggc gatggagatg tccttcatct ccaccagggg tgtcccgcga ttctcgtgga
     2521 ccttggccga acttgctttc ttggcgttca tgcgtgcctc ccctcacttc acccggcgcc
     2581 ggtagacctg gtcgacgaag acggcgaaga ccaggacgca gcccaccacg atgtcctttg
     2641 tggcggattc gaaattgagc agcgtcatgc ccgactgcag cgactgcatg accagggcgc
     2701 cgagcatggc gccgtagatg gtgccgaggc cgccggccag cgaagtgccg ccgatcaccg
     2761 cggccgcgat cacatagagc tcgtcgaact ggcccagcgc attggtggcc gagtcgaggc
     2821 gggccgagga gattgacgcc gagatggccg ccagaacgcc catcagcgcg aagatcatca
     2881 ccgtcagctt cttggtgttc acacccgcca gttctgccgc ttccggattg ccgcccgtgg
     2941 cgtagacgta gcggccgaag cgggtgcgcg tggcaagaaa gctcatgccg agcccgacgc
     3001 ccatggcgat cagtacggga accgagtagc cgaaggcgat gttgatgcct tccggcggca
     3061 cttcgattcc cgtggcctgc acgtagcgat ccacgacgcc cttgggccaa tagtaggaat
     3121 tcaccagcca ggtcacgccg agggcgagga cgcagcccgt gatggcgagg aaatactccg
     3181 cccacatcgg ccgctgcggg aagttgaagc gcttgcgctg aacccggttg ttgtagatgg
     3241 cgtagatgat ggccgcgcag atcacggcac ccaggatcca gctggccgtg gcgccgatgg
     3301 atccgggcgt gatgccagaa atgccgccgc cgatgagctt gaagttcttg tccatgggcg
     3361 cgacggtctc gccgcggatc accgcccatg ccgcaccgcg ccagacgatc aggccgccca
     3421 gcgtcacgat gaaggacggt atgccgagat aggcgatcag cgtgccatgg aaggcaccga
     3481 tggcgccccc aagcaacagg ccgaggatca aagtcacgat ccagatcgag ggatggccga
     3541 cgccgagcat aggccccagc cagaacacct gcatggtggc gatgcagacg gatgtgaaac
     3601 cgaggatcga ccccaccgac aggtcgatgt ggcgcatcac gatgatcagc accatgccgg
     3661 tggtcatcac cgcgatggaa gacgtctgca ccagcaggat ccacaggttg cgcggcgtga
     3721 ggaagctgcc gccgaacagg ccctcgccgt cgcgcaggaa accgctgtag atgtcgaaac
     3781 cgatccagat gatcagcagc gcggcgacca tgcccagcat gcgtgcgtca atctccgttg
     3841 cccggaggaa gcgtgcgatg gcgccctcat cgcttcgcgc cggattctgg gtattgtcca
     3901 cggctgtcat gaagtccctc cccaagtcgt ctgaccgctt gctgcggtgc ttctactaaa
     3961 aggaaaccgc gccgccctgt gaggcgacgc ggtccatcat gtttcaatcg tcagcaggat
     4021 gcaacgagat cagttgcagg ccgcgacggc accagcaggc gcaccggcgc agacttcttc
     4081 cttggtgacc cagccgccgt cgatgacgtc gttcaggttg tcgcgggtga tcgggttcgg
     4141 cgcgatcaga acggcgttga cttcaacgcc cttcgcgccg cccgagaact tgcccacgcc
     4201 cggaagttcg gtcgcggcct tgccgtcggc gatcatgttg gcgaattcgg cggcggcctt
     4261 gccgaggacg cgcgcgtcct tccagaccga caccagctgg gtgccaagcg ccacgcggtt
     4321 cagcgcggcc ttgtcgccgt cctgaccggt gacgggcacg gtgccggcaa ggccctgggc
     4381 ttcgagggcg gcaaccacgc cgccggccat gccgtcgttc tcggagatca cagcgtcgac
     4441 cttgttgttc accgaggtca ggcactgctc catgttcttc tgggccgcgt cgggcttcca
     4501 gccgtccgtg aaggtttcg
//
LOCUS       DBNGVR010000411         4312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_150679, whole genome shotgun sequence.
ACCESSION   DBNGVR010000411 DBNGVR010000000
VERSION     DBNGVR010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_150679"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="AC16A0_08720"
     CDS             complement(<1..758)
                     /locus_tag="AC16A0_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105458.1"
                     /translation="MASVVPKVPVRFDKVQKYFGAVKVLEEFDLDVAPGEFLVLLGAS
                     GSGKTTALRILSGLETATGGRVLIGDRDVTHILPKYRDISMVFQSYALYPHKTVAENI
                     GFPLKVRKIPQAEAEVAVRDAAAQVQIEQLLDRYPRQLSGGQRQRVALARAMVRRPSV
                     FLMDEPLSNLDAKLRVQMRVEIKELHQRLKTTTIYVTHDQIEAMTMADKIVVMHDGIV
                     EQIGAPLELYDRPANVFVAGFIGSPAMNLIEGVVR"
     gene            complement(762..1946)
                     /locus_tag="AC16A0_08725"
     CDS             complement(762..1946)
                     /locus_tag="AC16A0_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3105459.1"
                     /translation="MTNTSFKFEKSNRSLFERLFEPPEVGRMSPAANVVAHILLVLWT
                     IFVVFPIYWVVITSFKDAAAVNQGPFYVPFLDFQPNLDAWRAQFNSDANCDIPSILRQ
                     VGLLFYNAFVFVVSPVVTLQPMEPQLCKIYLAFTNSIVVSFAATAICVAIGSMAAYAL
                     ARVQYKPKFGNIVMFVLLTLLVILATSFAGIPWWLSSAVGLAMFFFLVRALGKHFRRT
                     LGNGDILFWIISQRILPPIVVIIPIYIMFQAVGLLDTHLALILVYAVANLPIVVWLMH
                     DFFVNLPIELEESAQLDGASRFAVFWDIVLPLTRPGLAATTLLTLILNWNEYLFAVFL
                     ATSKVQTMPIMVAAKNAGEKGVFWWEMCAIIVVMIIPVILMAILLQRFIAKGVLLGAV
                     KG"
     gene            complement(1943..3097)
                     /locus_tag="AC16A0_08730"
     CDS             complement(1943..3097)
                     /locus_tag="AC16A0_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3105460.1"
                     /translation="MSRADTMDQPGLSPAGLSQRETWAEWFDRHSRTFFIAPAVILIL
                     IFAIFPTFYSIVFALSRVRFTGNGLQFRFVGLQNFGKQFFGNDQVHFLGKVDQISIFG
                     LAFFVAVSAAVLWWLFRSVKKTTWVGMLGRLISAAMAIFIAWIAGATLLSGNTIGTLM
                     TTLFYVFVGCALQFVIGTALAFLCSQPISGKNFFRVVFFIPLMITPLGVGYAMKMVAD
                     ITKGPFEPIFRVFGIENWVWSADPWAARFVMMICDSWQWIPFIFICMLAALENVPRDH
                     VEAAQVDGASSVQIFREITWPQILPVAVTVLLIRMIEAFKIVDLPNIMTGGGPGSSTE
                     SMTLHSVYVWRANDIGTSAAIAYLLLILTVVVCASFFNYVVLGQLRRVQA"
     gene            complement(3109..>4312)
                     /locus_tag="AC16A0_08735"
     CDS             complement(3109..>4312)
                     /locus_tag="AC16A0_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105461.1"
                     /translation="AAGWGVELDAAGLLEDMDPYVAKDTKIDMADIAPYFREFGQKVG
                     GKTKLLMVDGDFQMVYYRKDVLEKAGLQPPKTWEEYLDVASKIHGQDMNGDGQADYGS
                     CIFKKRNAQSYFAIQTFAAPIVQTQGTAQGFHFDNATMKPIVNNDGWKKAFELYKETG
                     KYGPPEELNMDIGDTRAIFKAGRCGLLVEWGDPGTLQLDPDAAAVKGMIFAVGAVGSK
                     EVLDRATGKLVPVTKENAPHSVDGINYAPFAAFGGWAGAINAKADQKKKDAAYAFLSY
                     MNQAAQSNVDVTIGATGYNPYRNSQLQSTDLWVKAGMPADLAENYLGAINGALNNANM
                     ASDMKIPGAQKYTGVILDTELARYLAGEITVEEALKNIEAGWEEVTEDFGRDEQIKAQ
                     GLALGTGQ"
BASE COUNT          902 a         1280 c         1372 g          758 t
ORIGIN      
        1 cgcacgacgc cttcgatcag gttcatcgcg gggctgccga tgaagccggc gacaaagacg
       61 ttcgccgggc ggtcgtagag ttccagcggc gcgccgatct gctccacgat gccgtcatgc
      121 atcaccacga tcttgtcggc catggtcatg gcctcgatct ggtcgtgcgt cacatagatc
      181 gtcgtggtct tgaggcgctg gtgcagctcc ttgatctcga cccgcatctg cacgcgcagc
      241 ttggcatcga ggttggagag cggctcatcc atgaggaaga ccgaagggcg gcgtaccatg
      301 gcgcgcgcga gcgccacgcg ctgacgctga ccgcctgaaa gctggcgcgg ataacggtcc
      361 agaagctgct cgatctgaac ctgggcggcg gcatcgcgga cggcgacctc ggcctccgcc
      421 tgcggaatct tccgcacctt cagtggaaag ccgatgttct ccgcaacagt cttatgcggg
      481 tagagcgcat aggactggaa caccatcgag atatcgcggt acttgggcag gatatgcgtg
      541 acgtcgcggt cgccgatcag cacgcggccg ccggtcgccg tctcgaggcc ggagaggatg
      601 cgcaacgccg tggtcttgcc cgatcccgac gcacccagca gcaccaggaa ctcgcccggc
      661 gccacgtcga ggtcgaactc ctccagcacc ttcacggcgc cgaaatactt ctggaccttg
      721 tcgaacctga ccggaacttt cggaactacg cttgccatcc cttacccctt cacggcgccc
      781 agcagcacgc ccttcgcaat gaaacgctgg agcaggatcg ccatgagaat gaccggaatg
      841 atcatgacca cgatgatggc gcacatctcc caccagaaga cacccttctc gccggcattc
      901 ttggccgcca ccatgatggg catggtctgc accttcgagg tggcgagaaa cacggcgaaa
      961 agatactcgt tccagttcag gatcagcgtc agcagcgtgg tggccgcaag cccgggccgc
     1021 gtgagcggca ggacgatgtc ccagaacacc gcaaagcgcg aggcgccatc cagctgggcc
     1081 gactcctcga gttcgatcgg caggttgacg aagaaatcat gcatcagcca gacgacgatg
     1141 ggcaggttcg ccaccgcata gacgaggatc agagccaaat gggtgtcgag caatcccacg
     1201 gcctggaaca tgatgtagat cggaatgatc acgacgatcg gcggcaggat gcgctgagaa
     1261 atgatccaga agagaatgtc gccgttgccc agcgtgcgcc taaagtgttt gcccagggcg
     1321 cggaccagga agaagaacat ggcgaggcca acggcagagg acagccacca ggggattccg
     1381 gcgaagctgg tggcgaggat caccaggagc gtcagcagca cgaacatcac gatgttgccg
     1441 aacttgggct tgtattgcac ccgtgccagc gcataggccg ccatggagcc gatcgccacg
     1501 cagatggcgg ttgccgcgaa gctcaccacg atggaattgg tgaaggccag atagatcttg
     1561 cagagctgcg gctccatcgg ctggagcgtc acaacgggag acacgacgaa gacgaaggca
     1621 ttgtagaaca gaaggccgac ctgccgcagg atcgatggaa tatcgcaatt ggcatcgctg
     1681 ttgaactggg cgcgccaggc atcgaggttc ggctggaagt ccaggaaagg aacgtaaaag
     1741 ggcccctgat tgaccgccgc agcatccttg aaggacgtaa tgacgaccca gtagatcggg
     1801 aaaaccacga agatcgtcca gagcaccagc aggatatgcg ccacaacatt ggcggcaggc
     1861 gacatgcggc cgacttcggg cggctcgaag agcctttcga agaggctgcg attggacttc
     1921 tcgaacttga aggaggtgtt ggtcatgcct gcaccctccg cagttgcccg aggaccacgt
     1981 agttgaagaa cgaagcgcag acgacgaccg tcaggatgag gaggagatag gcgatcgcgg
     2041 ccgaggtgcc gatgtcgttc gcccgccaca catagacgga gtgaagcgtc atcgactccg
     2101 tcgacgaacc cggtccgccg cccgtcatga tgttcggcag gtcgacgatc ttgaaggcct
     2161 cgatcatgcg gatcagcagg accgtcaccg ccacgggaag gatctgtggc caggtgatct
     2221 cacggaagat ctggacgctc gaggcaccgt cgacctgtgc cgcctcgacg tgatcgcgtg
     2281 gcacgttctc cagcgccgcc agcatgcaga tgaagatgaa ggggatccac tgccaggaat
     2341 cgcagatcat catgacgaag cgggcggccc agggatcggc ggaccacacc cagttctcga
     2401 tcccgaagac gcgaaagatg ggttcgaagg gacccttggt gatgtcggcg accatcttca
     2461 tggcgtagcc gacgccaagt ggcgtgatca tcagcgggat gaagaagacg acgcggaaga
     2521 agttctttcc ggaaatcggc tgcgagcaaa ggaaggcaag cgccgtaccg atcacgaatt
     2581 gcagggcgca gccgacgaag acgtagaaca gcgtcgtcat cagcgtgccg atggtattgc
     2641 cggagagaag tgtcgcgccg gcgatccagg cgatgaaaat cgccatcgcg gccgaaatca
     2701 ggcggcccag catgccgacc caggtcgtct tcttgacgga gcggaacagc caccagagca
     2761 cggcggcgct gaccgcaacg aagaaggcca gaccgaaaat gcttatctga tcgaccttac
     2821 cgaggaaatg gacctggtcg ttgccgaaga actgcttgcc gaaattctgc agcccgacga
     2881 agcggaactg caggccgttg ccggtgaagc gcacgcggct cagcgcgaag acaatggaat
     2941 agaacgtggg gaagatcgcg aagatcagga tgaggatgac ggcgggcgca atgaagaagg
     3001 tgcgcgaatg gcgatcgaac cactcagccc atgtctctcg ctgagaaagt ccagccgggg
     3061 ataaccccgg ctggtccatg gtgtcggcgc gactcatcgt cgtgctgatt actggccagt
     3121 gcccagcgcg aggccctggg ccttgatctg ctcgtcgcgg ccgaagtcct cggtgacctc
     3181 ttcccagcct gcttcgatgt tcttcagggc ctcttccacc gtgatttcgc cggccagata
     3241 gcgggccagt tcggtgtcga ggatgacgcc cgtgtacttc tgcgcacccg ggatcttcat
     3301 gtccgaagcc atgttggcgt tgttgagggc accgttgatc gccccgagat agttctcggc
     3361 gagatcggcc ggcatgccgg ccttcaccca cagatccgtg ctctgcagct gcgagttccg
     3421 gtaagggttg tagcccgtgg cgccgatcgt cacgtcgacg ttcgactggg ccgcctggtt
     3481 catgtaggag aggaacgcat aagcagcgtc cttcttcttc tgatcggcct tggcgttgat
     3541 cgcacccgcc cagccaccga aggcggcgaa gggtgcgtaa ttgattccgt cgaccgagtg
     3601 cggcgcattt tccttggtca ccggcacgag cttgccggtg gcgcggtcga gcacctcctt
     3661 cgaaccaacg gcgccgacgg cgaagatcat acccttcacg gcggcggcat cgggatcgag
     3721 ctgcagcgtg cccggatcgc cccactccac cagcaggccg caacggccgg ccttgaagat
     3781 ggcgcgggtg tcgccgatgt ccatgttcag ctcttccggc ggaccgtact tgccggtctc
     3841 cttgtagagc tcgaaggcct tcttccaacc atcgttgttg acgatcggct tcatcgtggc
     3901 gttgtcgaag tggaagccct gggcggtgcc ctgggtctgc acgatcgggg ccgcgaaggt
     3961 ctggatggcg aagtaggact gagcattgcg cttcttgaag atgcaggagc cgtagtctgc
     4021 ctgaccgtcg ccgttcatgt cctgaccgtg gatcttggag gccacgtcga ggtattcctc
     4081 ccaggtcttg ggcggctgca ggccggcctt ctcgagcacg tccttgcggt aatagaccat
     4141 ctggaagtcg ccatcgacca tcagcaactt ggtcttgccg ccaaccttct ggccgaattc
     4201 gcggaagtag ggcgcgatgt cggccatgtc gatcttggtg tccttggcga catacggatc
     4261 catgtcttca aggagacctg cggcatcgag ttcgacgccc cagccggcgg cg
//
LOCUS       DBNGVR010000412         3514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4669298, whole genome shotgun sequence.
ACCESSION   DBNGVR010000412 DBNGVR010000000
VERSION     DBNGVR010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4669298"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC16A0_08740"
     CDS             <1..198
                     /locus_tag="AC16A0_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR3105462.1"
                     /translation="AHTPFEGDTVFAAATGLTVLPENRALALAGLGAMASRTLARAIG
                     RALWHAKPAGTVSSYREILGA"
     gene            271..849
                     /gene="infC"
                     /locus_tag="AC16A0_08745"
     CDS             271..849
                     /gene="infC"
                     /locus_tag="AC16A0_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR3105463.1"
                     /translation="MTGAIDHLSIPPRRPLSRGAAPPQKEEGHRINFRIESREVRLIG
                     ADGENLGVVPTRQALAMAEEANLDLVEVSPDAKPPVAKILHYGKFKFQEQKRAAEARK
                     KQKIIEIKEIKMRPMIDDHDYDVKMRAIRRFFEEGDKVKITLRFRGREMAHQELGQKL
                     LNRVKSDTAEIAKVESEPRFEGRQIVMVLSPR"
     gene            955..1458
                     /locus_tag="AC16A0_08750"
     CDS             955..1458
                     /locus_tag="AC16A0_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR3105464.1"
                     /translation="MFDDGIPIARRSALFLIAAAIFGAAVRPAAAKDDDDSDSDNSGS
                     GGGGSNSGSGGGDDSDSDSDSDSDSDSDSDDGGGSGRSGSGRRSDDDNDDDRIRAAVR
                     GGEAAPLRDILAVVRKSHRGKIVNIRLTGSGNRLAYRIRMLDSANRLIELRVNAKTRK
                     ITSVRKL"
     gene            1458..2123
                     /locus_tag="AC16A0_08755"
     CDS             1458..2123
                     /locus_tag="AC16A0_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3105465.1"
                     /translation="MRILIVEDEQRIANDIAEAVSGGGYVPEIVGDGEDAWFQGGTEA
                     YAAIVLDLGLPRLDGLTILKRWRKEGVTTPVLVLTARGAWDERVDGIDAGADDYLPKP
                     FRMEELMARLRAVLRRASGRAEPAVTIGPVNLDPRTRQVTVNGVPVSLTPLEFRLLNV
                     LFHHRGRVVSQAELSDNLYSHDSERDSNAIEALIGRLRRKLKADVIETRRGFGYIVVD
                     GGA"
     gene            2120..3427
                     /locus_tag="AC16A0_08760"
     CDS             2120..3427
                     /locus_tag="AC16A0_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3105466.1"
                     /translation="MTTGSLRLRLLLAAVLSISLALTLTGLALVSLFEQQIRDRITAE
                     LEGDLLQLVGAIEVAPDGKLGLARELGNTRYNEPYGGRYWRISEAAAAGVTPPEPLRS
                     RSLWDSDIDPANPVGPEGEPLILASRSVSVGETAPKALLFVIAAHQDEIDRPLGQLRD
                     QLILSFLLIGALLVLGAWIQVSVGLRPLARLRQQLARVRSGEEQGLSGSFPAEVAPLV
                     GELNDVLAMREKSLDRARRRAGDLAHGLKTPLTVLQAVARDIRGHKLAEEAAEIEEQA
                     DAMNRHIERALVRARLSSGRGHAAANLETVARKVADTLSRMPGAEDLDFDIRVPAGTR
                     IPMEQGDLTELLGNLMDNGRKWAASRVRLRFEDGAITIEDDGPGVPDAELERIRERGR
                     RLDETKQGSGLGLSIVEDIADIYGLQLIYGRSELGGLKVTVRL"
BASE COUNT          640 a         1132 c         1152 g          590 t
ORIGIN      
        1 gcgcatacgc ccttcgaggg cgacaccgtc ttcgcggctg ccacgggcct taccgttctt
       61 cccgagaacc gtgccctggc ccttgccggt ctcggggcca tggcgtcccg caccctggcc
      121 cgcgccatcg gccgggccct ctggcatgcg aagccggccg gcaccgtttc gagttatcgc
      181 gaaattctgg gcgcttgacg ccgggcgtcc tacaatgtct atagctggcg caaccaaacc
      241 aggaccggcc gagaggattc gggccgtccg atgacaggag cgatcgacca cttgagcata
      301 ccccccagga ggccactgag ccgcggagcc gcccccccgc agaaggaaga aggacacagg
      361 atcaatttcc gcatcgaatc ccgcgaggtg cgcctcatcg gagcagatgg agagaacctc
      421 ggcgtggtgc cgacccgcca ggcgcttgca atggctgagg aggccaatct cgacctggtc
      481 gaagtttccc ctgacgccaa gccgccagtc gccaagatcc tccactacgg gaagttcaag
      541 tttcaggaac agaagcgtgc ggccgaggcc cgcaagaagc agaagatcat cgagatcaaa
      601 gagatcaaga tgcgcccgat gatcgacgat cacgactacg acgtgaagat gcgcgccatt
      661 cgccgcttct tcgaggaagg cgacaaggtc aagatcaccc tgcgcttccg cggccgtgaa
      721 atggcgcacc aggaactggg ccagaagctt ctcaaccggg tcaagtcgga tacggccgaa
      781 atcgccaagg tcgaatccga accgcgcttc gaaggccgcc agatcgtcat ggtcctttcg
      841 ccccgctgac aaatcgctga caggcacgtt cacgcgcctc tcatcttccg gattctagta
      901 caattcgatc cgtcggcagg gtatcgtccc cgcggacggt tgagagttgc gtaggtgttt
      961 gacgacggca tccccatcgc gcgccggagt gcgctgttcc tgatcgctgc tgccatcttc
     1021 ggcgcggccg tgcgacctgc tgccgcaaag gatgatgacg acagcgacag tgacaatagc
     1081 ggcagtggcg gcggcggcag caatagcggc agtggcggcg gagacgatag cgacagtgat
     1141 agcgacagcg acagcgatag tgatagcgac agtgacgatg gcggcggcag cgggcgcagc
     1201 ggttctggcc gacgcagcga cgacgacaat gatgatgaca ggatccgtgc ggccgttcgt
     1261 ggaggcgagg ccgcgccatt gcgcgacatc ctggccgtcg tgcgcaagtc ccatcgcggc
     1321 aagatcgtca acattcgact gaccggctcc ggaaaccgcc tggcctatcg catccgcatg
     1381 ctggacagtg cgaaccggct gatcgaactc agggtcaatg ccaagacccg gaagatcaca
     1441 tccgtacgga aactctgatg cgcattctca tcgtggaaga cgaacagcgg attgcaaacg
     1501 atatcgcgga ggcggtgagc ggcggcggct acgtaccgga gattgtcggc gatggtgagg
     1561 acgcatggtt ccagggcggc accgaggcct atgccgccat cgttctcgat ctcggcttgc
     1621 cccggctcga tggactcacc attctcaagc gctggcgcaa ggaaggcgtg acgactcccg
     1681 ttctggtgct gacggcacgg ggcgcctggg acgaacgcgt cgatggcatc gatgccggtg
     1741 ccgatgacta cctgcccaaa cccttccgca tggaggaact catggcccgg ctgcgcgccg
     1801 tgctgcggcg tgcttcgggc cgggcggagc cggccgtcac catcgggccg gtcaatctcg
     1861 atccgcgcac ccgccaggtc acggtgaacg gcgtgccggt ttcgctgaca ccgctggaat
     1921 tccgcctgct gaacgtgctc ttccatcatc gcggccgtgt tgtctcgcaa gcggaactga
     1981 gcgacaatct ctactcccac gacagcgagc gcgactccaa tgccatcgag gccctgatcg
     2041 gccgcctgcg gcgcaagctg aaggccgatg tgatcgaaac ccggcgcggc ttcggttaca
     2101 tcgtggtaga tggaggggca tgactacagg ctcgctccgc cttcggttgc tccttgcggc
     2161 ggttctgtcg atttcgctgg ccttgacgct gaccggcctg gcactggtga gcctcttcga
     2221 acagcagatc cgcgaccgca tcacggccga gcttgaaggc gacctgcttc agctggtggg
     2281 tgccatcgag gtcgctccag atggcaagct cggccttgcg cgggaactcg gcaatacccg
     2341 ctacaacgag ccctacggcg gccgctactg gcggatctcc gaggcggcgg ccgcaggcgt
     2401 cacgccgcct gagccgctgc ggtcgcggtc cctgtgggac tcggacatcg atccggcaaa
     2461 cccggtcggt cccgagggcg agcccctgat cttggccagc cgcagcgtct cggttggcga
     2521 gacggcaccc aaggcgctgc tcttcgtcat cgccgcccac caggacgaga ttgaccgtcc
     2581 gctggggcag ctgcgcgatc agctgatcct gtccttcctt ctgatcggcg cgctgctggt
     2641 gctgggcgcc tggattcagg tcagcgtcgg gctgcgtccg ctggcccggc tgcgccagca
     2701 gctggcccgc gtgcggtccg gcgaggagca ggggctgagc ggcagttttc ccgcggaagt
     2761 ggcgccgctg gtgggcgaac tcaacgacgt gctcgccatg cgcgagaagt cactcgaccg
     2821 cgcccgccgc cgtgccggcg atctcgccca tgggctgaag acccccttga ccgttctgca
     2881 ggcggtcgcg cgcgacattc gtggccacaa gcttgccgag gaggcggccg agatcgagga
     2941 acaggccgat gccatgaatc gtcacatcga gcgggcgctg gtccgcgcgc gtctctccag
     3001 cggacggggc cacgcggccg ccaatctgga gaccgtcgca aggaaggtgg cggataccct
     3061 gtcgcgcatg ccgggagcgg aggacctcga cttcgacatc agggtgcctg ccgggacccg
     3121 cattcccatg gaacagggcg acctcacgga gcttctcggc aacctgatgg acaatggccg
     3181 caaatgggcc gcatcgcgcg tgcggctgcg cttcgaggac ggcgcgatca ccatcgagga
     3241 cgacggcccc ggcgtgccgg atgccgaact ggagcgaatc cgcgagcgcg gccggcggct
     3301 ggacgaaacc aagcagggat cgggcttggg cctgtcgatc gtcgaagaca ttgccgacat
     3361 ctatggcttg cagctgatct atgggcgctc cgagctcggt ggactgaagg tcactgtccg
     3421 gctctgactt gcatttcccg gcggattttg cttaaacccg cccgttcaag ccgcctggcc
     3481 tgtaaagggc atgccatggc ggcttaataa tgct
//
LOCUS       DBNGVR010000413         3865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4669509, whole genome shotgun sequence.
ACCESSION   DBNGVR010000413 DBNGVR010000000
VERSION     DBNGVR010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4669509"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="AC16A0_08765"
     CDS             complement(<1..767)
                     /locus_tag="AC16A0_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3105467.1"
                     /translation="MNHLIAAPLLLPPLVGAIMIMSMRHHLELARIFSVASISLLLLI
                     NIWLLAQSGAGAIQTYELGAWPAPFGIVLVLDRLAAIMLVLTAAIALAVVVYAIGSGW
                     DRRGRHFHALLQFQVMGLSGAFLTGDLFNLFVFFEVLLIASYGLMVHGGGQKRLQAGV
                     QYVVYNLLGSTLFLFALGTIYAVTGTLNMADLAVRVAEIPADNTALIRVAAVLLLLVF
                     AIKAALVPLHFWLPSTYAEAPGPVAALFAIMTKVGVYA"
     gene            complement(764..1114)
                     /locus_tag="AC16A0_08770"
     CDS             complement(764..1114)
                     /locus_tag="AC16A0_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HIR3105468.1"
                     /translation="MEYLVSIAVGVLAAGGTYLVLRLRTFPVILGISLLSYAVNLFLF
                     SSGRLALNLPPIWTGDTAAYTDPLPQALVLTAIVISFGMTAVVVMLALSSYLTAHDDR
                     IDTREDDGGGRDQA"
     gene            complement(1115..>3865)
                     /locus_tag="AC16A0_08775"
     CDS             complement(1115..>3865)
                     /locus_tag="AC16A0_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HIR3105469.1"
                     /translation="VTGTALIGLLTHAPEVFSGGVVKEKLDWLPVMGLNANFFLDGLG
                     FLFAALILCIGLLIIIYARFYLHRDDPMGQLYTYLLLFQGAMVGIVLSDNILLLLIFW
                     ELTSLTSFLLIGYWKHLPEGRQGAVMALTVTGMGGLALIGGLLILGEIAGSYDLTEIL
                     AKKAAVQASPLYLPALVLILLGCFTKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKA
                     GLFLMARLWPVLAGTEEWFYLVATTGLVTMLAGAFIGLFKNDLKALLAYSTVSHLGLI
                     TMLLGFGTPMAAVVAVFHIINHATFKAPLFMVAGIIDHEAHTRDIRRLGGLRHLMPLT
                     FGIATIAALSMAGIPPLNGFISKEMMLEEAAHTDWLGSAWLVPGLATLGALISAAYSF
                     RFIAHVFLGPVRHDYPARPHDPPIGFWIAPAVLATLVVAIGLQPALVAGPLVALASGA
                     VIGEAPPAYTLKLWHGLTPALLMSAIATAGGLAVLAGYRPLDRLWLRGPRLEAKSIFD
                     AVLDRLVGWAGSLTQALHDGAMTRYTAIFMATVVLAGTGAWFGGAGPSVAREFLPVGP
                     VPAIGGLLLLVATGCIVAFHHNRLLALVFIGVIGLIVSLAFAYLSAPDLALTQASVEV
                     VTIILLLLALNFLPKGTPHESSGSRRLRDGILASLVGLGAGLLTFQIQASNFSQASIS
                     AYHLANAKPGGGGTNVVNVILVDFRGFDTFGEIIVLGIAALVIYALTETLLAGAVGRR
                     LADRPPDTGEAGDSHPLMMVIATRVMMPITLMVALFIFLRGHNQPGGGFIAGLVVAIA
                     LIMQYMASGFDWAAARQKYPYHGIIAAGIFAALLTGLGSLVLGYPFLTTSYGYFKLPF
                     IEKFELATAMAFDAGVFLCVVGAVMLALESLSRLAHRSGERPSPYPMDVDPSRTAQPE
                     KA"
BASE COUNT          849 a         1148 c         1295 g          573 t
ORIGIN      
        1 gcgtagacgc cgaccttcgt catgatcgcg aaaagcgcgg caacagggcc tggcgcctcc
       61 gcataggtgg atggcaacca gaagtgaagc gggaccagcg cggccttgat cgcaaacacc
      121 aggagcaaga ggacggcagc gacccggatg agggccgtgt tgtcggcggg gatctcggcc
      181 acccgcacgg cgaggtcagc catgttcagc gtgccggtca cagcatagat cgtaccgagc
      241 gcgaagagaa acagtgtgga gccgagcagg ttgtagacca catactgcac gccggcctgc
      301 agccgcttct gcccgccgcc gtgaaccatg agtccgtagg acgcgatcag caagacctcg
      361 aagaacacga agaggttgaa aaggtccccg gtcaggaagg cgccggacag tcccatcacc
      421 tggaactgca ggagcgcatg gaagtggcgc ccgcgacggt cccaccccga tccgatggcg
      481 tagacaacca cggccagtgc gatcgccgcc gtcaacacga gcatgatagc cgccagccgg
      541 tcgagcacca gcacgatgcc gaagggcgcc ggccatgcac cgagttcgta ggtctgaatg
      601 gcgcctgcgc cgctttgcgc aagaagccag atgttgatca ggaggagaag cgagatgctc
      661 gcgaccgaga agatgcgcgc cagctccaga tgatggcgca tcgacatgat catgatggcg
      721 cctaccagcg gtggcagcag aagaggagcc gcgatcaggt ggttcatgcc tggtcccttc
      781 cgcccccatc gtcttcgcgg gtatcgatcc ggtcgtcgtg cgcggtgaga taggagctga
      841 gcgccagcat gacgacgacg gctgtcatgc cgaaggaaat gacaatcgcg gtgaggacga
      901 gggcctgcgg cagtgggtcg gtataggctg ccgtgtcgcc ggtccagatc ggcggcagat
      961 tcaaggcaag tcttcccgac gaaaacagaa agaggttcac cgcataggat aggagcgaga
     1021 tccccaggat cacgggaaag gtcctcaggc gcaggacgag gtaggttccg ccggcggcaa
     1081 gaactccgac ggcgatggaa accagatact ccatctacgc tttctccggt tgcgccgtac
     1141 gggacgggtc gacatccatc gggtaggggc tcggtcgctc gccgctgcga tgcgccagcc
     1201 gcgacaggct ctccagtgcc agcatcacgg cacccacgac acagagaaag acgcccgcgt
     1261 cgaaggccat tgccgtggca agctcgaatt tttcgatgaa gggaagcttg aagtagccat
     1321 agctggtggt caggaaggga tagccgagga ccaacgatcc caggcccgtc aggagggctg
     1381 cgaagatgcc cgcggcaatg atgccgtggt atgggtactt ctgccgcgcc gccgcccagt
     1441 cgaatccgct ggccatgtat tgcatgatga gtgcgatcgc gaccaccagg ccggcgatga
     1501 agccgccgcc gggctgattg tggccacgga ggaagatgaa cagcgcgacc ataagcgtga
     1561 tcggcatcat gacgcgcgtg gcgatcacca tcatgagcgg atggctgtcg cctgcctcgc
     1621 cagtatcggg cggccggtct gccagccttc tgccgacggc tccggcgagt agcgtctctg
     1681 tgagcgcgta gatgaccagt gcggcgatgc caagaacgat gatctcgcca aaggtatcga
     1741 agccgcggaa gtcaaccagg atcacgttga cgacattggt gccgccgccg ccgggcttgg
     1801 cattggccag atgataggcg gagatgctcg cctgggagaa attggacgcc tggatctgaa
     1861 aggtcagcag gcctgcgcca agcccgacca gtgaagcgag gatgccgtcg cgcaggcgcc
     1921 ggctgccgct gctttcgtgt ggcgtgcctt tcggcaggaa gttgagcgca aggagaagca
     1981 ggatgatcgt cacgacctcg accgaggctt gcgtgagagc aagatccggg gccgagagat
     2041 aggcaaaggc cagagagacg atgagtccga ttacgccgat gaagacgagc gccaagagcc
     2101 ggttatggtg aaaggccaca atgcagccgg tcgcgaccaa cagcagcaat ccgccgatcg
     2161 cgggaacagg gccgaccggg aggaattccc gcgcaacgct cggaccggca cccccgaacc
     2221 aggcgccggt ccctgccagg acgaccgtgg ccatgaagat ggccgtatag cgggtcattg
     2281 cgccatcatg cagcgcctgg gtcaggctgc ccgcccatcc gaccagccgg tccagaactg
     2341 cgtcgaagat cgacttggcc tcaagccttg ggccgcgcag ccagagccga tcgagcgggc
     2401 gatagccggc cagcacggcc agtccgccag cggtggcaat cgccgacatg agaagtgcgg
     2461 gcgtgagccc atgccagagc ttcaacgtgt aggccggcgg cgcctctccg atcacggcac
     2521 ccgatgcaag cgccaccagc gggcctgcga ccagggctgg ctgaagcccg atcgccacca
     2581 cgagagttgc cagcacggcc ggcgcaatcc agaatccgat tggcgggtca tggggcctgg
     2641 cggggtagtc gtggcgcacc ggtcccagga agacatgtgc aatgaaccgg aaggaatagg
     2701 ccgccgatat gagcgcaccg agtgtggcaa ggcctggcac cagccacgcc gaccccagcc
     2761 agtcggtgtg ggcggcctct tcgagcatca tttccttcga gatgaaaccg ttcagcggcg
     2821 gaatgccggc catggaaagg gccgcgatgg ttgcgatgcc aaatgtcagc ggcatcaggt
     2881 gccgcaggcc gccaagacgc cggatgtcgc gggtatgggc ctcgtgatcg atgatgccgg
     2941 cgaccatgaa caagggcgcc ttgaaggtgg cgtgattgat gatgtggaac accgcgacaa
     3001 cggccgccat cggcgttccg aagccaagca gcatcgtgat caggccgaga tggctgacgg
     3061 tggagtaggc aagcagcgcc ttcagatcgt tcttgaacag gccgatgaag gcgcctgcca
     3121 gcatcgtcac caggcctgtc gtggcaacca ggtagaacca ctcctctgtc ccggccaata
     3181 ccggccagag ccgggccatg agaaacagtc ccgccttcac catggtggcg gagtgcagat
     3241 aggccgaaac tggcgtgggt gccgccatgg catgcggcag ccagaagtga aacggaaact
     3301 gggcggactt ggtaaagcag cccagaagaa tcaggaccaa tgcaggaagg tagagcgggc
     3361 ttgcctgcac cgcggccttc ttcgcaagga tctcggtcag gtcgtagctg cccgcgatct
     3421 cgccgaggat gagcagaccg ccgatcaggg ccagtccgcc catgccggtc accgtgagcg
     3481 ccatgacggc cccttgccgg ccttccggca ggtgtttcca atagccgatg agcaggaatg
     3541 aggtgagcga ggtgagttcc cagaagatca ggagaagcag gatattgtct gacagcacga
     3601 tgccgaccat cgcgccctgg aacagcagca gataggtata gagctggccc atcggatcgt
     3661 cccggtgcag gtagaaccgg gcataaatga tgatcagcag gccgatgcag aggatgagcg
     3721 ccgcgaacag gaagccaagc ccgtcgagga agaagttcgc gttgagcccc atgacgggaa
     3781 gccagtccag cttttccttc acgactccgc cggaaaacac ttcaggagca tgagtcagca
     3841 ggccgatcag ggccgtccct gtcac
//
LOCUS       DBNGVR010000414         2604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_942245, whole genome shotgun sequence.
ACCESSION   DBNGVR010000414 DBNGVR010000000
VERSION     DBNGVR010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_942245"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /gene="prfB"
                     /locus_tag="AC16A0_08780"
     CDS             <1..704
                     /gene="prfB"
                     /locus_tag="AC16A0_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_111199041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3105470.1"
                     /translation="SMLMRMYMRYANKYGYKAEVLDEHPGEEAGIKSCTLQIKGHNAY
                     GKLKTESGVHRLVRISPYDSNARRHTSFASVWVYPVVDDNIEIEVVESDLKIDTYRAS
                     GAGGQHINTTDSAVRITHVPTGIIVACQAERSQHKNRATAMNMLKARLYEMELQKQQE
                     KIDAANAKKTEIGWGHQIRSYVLQPYQLVKDLRTGHTSTSPGDVLDGEVEDFIQASLA
                     HKVKGGGDQPIEDID"
     gene            complement(705..1223)
                     /locus_tag="AC16A0_08785"
     CDS             complement(705..1223)
                     /locus_tag="AC16A0_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR3105471.1"
                     /translation="MRPATIRVWDPLVRLFHWSLVGAMAYEFLFEPGTFTHNAVGYAI
                     LGLIAVRILWGLIGPRHARFRDFVTSPVATLNYVIEIVRGHPRRYIGHNPAGAMMVLG
                     LMLAVTATAASGWAMTTDALWGSEWIEELHEALAYGTLALIALHVLGVIAASWQHGEN
                     LVKSMLTGRKRA"
     gene            complement(1262..1528)
                     /locus_tag="AC16A0_08790"
     CDS             complement(1262..1528)
                     /locus_tag="AC16A0_08790"
                     /inference="COORDINATES: protein motif:HMM:NF025051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR3105472.1"
                     /translation="MRKFAIASTLTLAVLSCAGAAQASEICKAHDATEWMSKDAITAK
                     VTAMGYEVRKVQEEDGCWEVKGMKDGKRVEAYFDPVTAELVLTK"
     gene            1729..>2604
                     /locus_tag="AC16A0_08795"
     CDS             1729..>2604
                     /locus_tag="AC16A0_08795"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3105473.1"
                     /translation="MLNLDPQIYVDPAVLPRERNTIFARTWQLLGPASQVKERGQYVS
                     ADIAGIKVFAIRGRDGSLRAFRNVCRHRGAQLLPEGEGRCATIRCPYHQWVFADDGYL
                     VNAPWFGEDPDFDPSQWPLESIQVDVWRGLLFVSVDPQEDLVTQLGELVAELADEPIE
                     SYHAVKNDRVVFDANWKIYTDNFVEGYHIPGVHPNFFAAIDFEQFKTTAHKGFIRMTA
                     PPREGLFYRGKWLWMWPNWTLSLFEGGMNTSRINPTSTDRTEQLYHFYFADTSPEHDE
                     SRSDTIARNLAVVRED"
BASE COUNT          519 a          820 c          796 g          469 t
ORIGIN      
        1 cctcgatgct gatgcgcatg tatatgcgct atgccaacaa atacggctac aaggccgagg
       61 tgctggacga gcacccgggc gaagaagcgg gcatcaagtc ctgcacgctg cagatcaagg
      121 gccacaatgc ctatggcaag ctcaagaccg agtcgggcgt gcaccggctg gtgcgcatct
      181 cgccctatga ctcgaacgcc cgccgccaca cctccttcgc ctccgtctgg gtctatcccg
      241 tggttgacga caacatcgag atcgaagtcg tcgagagcga tctgaagatc gacacctacc
      301 gggcgagcgg cgcaggcggc cagcacatca acaccaccga ttcggccgtg cgcatcactc
      361 acgtgccgac gggcatcatc gtggcctgcc aggccgagcg ctcgcagcac aagaaccgcg
      421 ccaccgccat gaacatgctg aaggcgcggc tctacgaaat ggaactgcag aagcagcagg
      481 aaaagatcga cgcggccaac gccaagaaga ccgagatcgg gtgggggcac cagatccggt
      541 cctatgtgct gcagccatat cagctggtga aggacctccg taccgggcac accagcacaa
      601 gcccgggcga cgtgctcgat ggcgaagtcg aggacttcat ccaggcctcg ctggcgcaca
      661 aggtcaaggg cggcggcgac cagccgatcg aggacatcga ctgatcaggc gcgcttgcgc
      721 ccggtcagca tggacttgac gagattctcg ccgtgctgcc agcttgcggc gatcacgccg
      781 agcacatgca gggcgatgag cgcgagggtg ccataggcga gggcctcatg cagttcctcg
      841 atccactcgc ttccccacag cgcatcggtt gtcatggccc agccgcttgc ggcggtcgcg
      901 gtgacagcca gcatcagtcc cagcaccatc atggcgcccg ccggattgtg accgatatag
      961 cggcggggat ggcccctgac gatctcgatc acatagttga gtgttgcaac agggctggtc
     1021 acgaagtcac gaaagcgggc gtggcgcggt ccgatcagtc cccagagaat ccggacggca
     1081 atcaacccaa ggattgcgta gccgacggcg ttgtgagtga aggtgcctgg ctcgaagagg
     1141 aactcatagg ccatggcgcc aaccagcgac caatggaaca ggcgcaccag cggatcccag
     1201 acccggatgg ttgcgggccg catcgcgcgg cccgcctcca tcatactgac ccgggacggc
     1261 attacttggt cagcacgagt tccgcagtca cgggatcgaa gtaggcttcg acgcgcttgc
     1321 catccttcat gcccttcact tcccagcaac catcctcttc ctgaaccttt cggacctcgt
     1381 agcccatcgc cgtaactttg gcggtaatgg catccttgga catccactcg gtcgcatcgt
     1441 gagccttgca gatttcgctg gcctgggcag cgccggcgca ggagaggacg gcgagggtga
     1501 gggtcgaagc gattgcgaac ttgcgcatga tatctccaat tgtctggcgc tgatcgttca
     1561 gcgcggggga aatctgcgct gcttcctgtg atggatggct gacggcctct gtcagcatcg
     1621 tatcagcggc cggtaagact catggcaaat gcaagcctcg cccctacagg gcgaggcttg
     1681 tcgcgggcgg cgggattagg cagtctctcc cggaaaggac gtctggccat gctcaacctt
     1741 gatccgcaaa tctatgtcga ccccgctgtc ctgccgcgcg agcggaacac gatcttcgcg
     1801 cggacatggc aattgctcgg tcccgccagc caggtgaaag agcgcgggca atatgtctcg
     1861 gccgacattg ccggaatcaa ggtcttcgca atccgagggc gggacggcag tttgcgggcc
     1921 ttcaggaacg tgtgccgcca ccgcggcgcc cagcttcttc ccgagggcga ggggcgctgc
     1981 gccacgatcc gctgccccta ccatcagtgg gtttttgccg atgacggcta tctggtgaat
     2041 gcgccatggt tcggcgagga tccggacttc gatccgtccc aatggccgct cgaatccatc
     2101 caggtggatg tctggcgtgg tctgcttttc gtttctgtgg atccgcagga agacctggtc
     2161 acgcagctgg gtgaacttgt ggcggaactt gccgatgagc ccatcgagtc ctatcacgcg
     2221 gtgaagaacg accgcgttgt cttcgatgcc aattggaaga tctacaccga caatttcgtc
     2281 gagggctatc acatccccgg cgtgcatccg aacttctttg cggcgatcga cttcgaacag
     2341 ttcaagacca ccgctcacaa gggtttcatc cgcatgacgg ccccgccgcg cgagggtctg
     2401 ttctatcgcg gcaagtggct gtggatgtgg ccgaactgga cgctctcgct cttcgagggc
     2461 ggcatgaaca cctcgcgcat caacccgacc agcaccgacc gcaccgagca gctctatcac
     2521 ttctatttcg ccgatacctc gccggagcat gacgagtccc gcagcgacac catcgcgcgc
     2581 aacctcgccg tggtgcgtga ggac
//
LOCUS       DBNGVR010000415         3525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_742384, whole genome shotgun sequence.
ACCESSION   DBNGVR010000415 DBNGVR010000000
VERSION     DBNGVR010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_742384"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="AC16A0_08800"
     CDS             complement(<1..117)
                     /locus_tag="AC16A0_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3105474.1"
                     /translation="MKLKDRIAIVTGASKGIGLACAERFAADGATVIMSDVDD"
     gene            complement(136..876)
                     /locus_tag="AC16A0_08805"
     CDS             complement(136..876)
                     /locus_tag="AC16A0_08805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3105475.1"
                     /translation="MSFSGKRVIVTGAGRGLGAAFAVILADQGAEVIMTGRSMENLTS
                     LAESIRLRTGKRPETLSLDMADVSAVTLLAKKMRDEGRPLDILVNNAAQWLPGRMDEH
                     DALAISQTITGIVTGTLLFTRGLVPLLEKSGAGDILNVISISGIPNTPLHGASVAYFA
                     AKHGQAGMNDGLRQELKGRPIRVSAVYPSNITDISPLESEAWKAVRPAQSWATNRDVV
                     EASLFALSRPRHVTLASIVIDPDNGGLF"
     gene            complement(889..2034)
                     /locus_tag="AC16A0_08810"
     CDS             complement(889..2034)
                     /locus_tag="AC16A0_08810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3105476.1"
                     /translation="MTFIDAVRARWAVGAMFLLHGILIGSWVPHIPLAKERLDAGPAV
                     FGLALLAFAGGAVVAMPTAGMLINRFGSARMSGITGILFCLTFFGPILAPDMTTFVVA
                     GFLMGMSIGSMDVAMNAHGIAVEKALRLPTMSMFHGGFSLGGMIGAFLGALLLRLYGE
                     LPQAAMTAALCLAMQLTAMRFLLPNSVDQGLSGSHFAWPTRATIGLGLLCFLALMIEG
                     SILDWAAIMMREKFLVDASIAALAFGFYQSGMAVARFTGDWLRLKVGAVRLVFVSALM
                     AAAGTAVALAVPSPEVAIAAFVFAGLGIGNVAPILFAGGGRLEPDAPGRGIAAVTTLG
                     YSGFLAGPPLIGFAAQLTSLPVALGLTVIAALIIAAFARMVDAADSF"
     gene            2172..2966
                     /locus_tag="AC16A0_08815"
     CDS             2172..2966
                     /locus_tag="AC16A0_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HIR3105477.1"
                     /translation="MSRDEAAPAYVPPPAIKIKEIGVDDIWAALRAGWRDFTSAPLIG
                     LFFGAIYVAGGILILLLLSVYHQPWWIIPIAVGFPLIGPFVAVGLYEVSRRLAAGQPI
                     VWGEVLSVIWAQRSRQIGWMAFVVLFIFWMWLYEVRMLLAIFLGFKSFSSIDAFLTVV
                     TTTAEGLGFLAVGTVVGAFLSFILFCTTVVAIPLLLDRETDLITAIIASFRSVFRNPA
                     PMIGWGIIVATLTALALLPVFLGLLVVLPVLGHATWHLYTRVVAPP"
     gene            complement(2967..>3525)
                     /locus_tag="AC16A0_08820"
     CDS             complement(2967..>3525)
                     /locus_tag="AC16A0_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR3105478.1"
                     /translation="LPLPPSCLPTLWGNDERFRKSYLSHFPGYYETADAGYMDEDGYL
                     YIMARTDDIINVAGHRLSTGGMEEVLAGHPSVAECAVIGIADALKGQVPLGLVVLKSG
                     VTKPAADIETECVGLVREKIGAVAALRTVAVVPRLPKTRSGKILRGTMRKIADKEAFK
                     TPATIDDPAILDEIRAAMQAKGVGV"
BASE COUNT          614 a         1109 c         1184 g          618 t
ORIGIN      
        1 gtcgtcgaca tcgctcatga tcacggtggc gccgtcggcg gcaaagcgct cggcacaagc
       61 aagcccaatg cccttcgacg cgccggtgac aatggcgata cggtctttca gtttcacgat
      121 gaactcctgc tggatctaga agaggccgcc attgtcgggg tcgatgacga tcgacgccag
      181 cgtgacatgg cgcggccgcg acagcgcgaa gagcgaggct tcgacgacgt cgcggttggt
      241 ggcccaggac tgcgcgggac ggacggcttt ccaggcttcg gactcgagcg gcgagatatc
      301 ggtgatgttg gacgggtaga cggcgctgac gcggatgggg cgtcccttca gctcctgccg
      361 caatccgtca ttcatgccgg cctggccatg cttggcggcg aaataggcga cggaggcgcc
      421 atgcaggggc gtattgggaa tgccggagat ggaaatgacg ttgagaatgt cgcccgcgcc
      481 ggacttctcg agcaagggca ccagtccgcg cgtgaacagc agggtcccgg tgacgatgcc
      541 agtgatggtc tgactgatcg ccagcgcatc gtgctcgtcc atccggcccg gcagccactg
      601 ggccgcattg ttgaccagga tgtcgagcgg gcggccctcg tcgcgcatct tcttggccag
      661 cagcgtgacg gcgctcacgt cagccatgtc gagcgaaagg gtctcggggc gcttgccggt
      721 gcgcagccgg atcgattcgg ccagcgaggt gaggttctcc atgctgcggc cggtcatgat
      781 gacctccgcg ccctggtcgg ccaggatcac ggcaaaggcc gcgccaaggc cgcggccggc
      841 cccggtgacg atcacacgct ttccggagaa actcatgggt tgattaactc agaagctgtc
      901 tgctgcgtca accattctcg caaaggcggc aatgatgagc gcggcgatca cggtgaggcc
      961 aagggcaacc ggcaggctgg tgagctgcgc ggcaaagcca atgagtggcg ggccggcgag
     1021 gaagcccgag tatccgagag tggtgacggc ggcgattccg cggccgggcg catcgggttc
     1081 gaggcgcccg ccgccggcga agaggatcgg tgccacattg ccgatgccca gtccggcgaa
     1141 gacgaaggcc gcgatcgcca cctccggcga tggcacggcc agagccacgg cggttcctgc
     1201 cgcggccatc aacgcgctga cgaagacaag acgtacggcg cccaccttga gacgcagcca
     1261 gtcgccggtg aagcgggcaa cggccatgcc gctctggtag aagccgaagg cgagggcggc
     1321 gatgctggca tcgaccagga acttctcgcg catcatgatg gcggcccagt cgaggatcga
     1381 cccctcgatc atgagcgcca ggaaacacag cagcccgagg ccgatggtgg cccgtgtcgg
     1441 ccaggcgaaa tgggagcccg aaagcccctg gtcgacggaa ttgggaagca ggaatcgcat
     1501 cgcggtgagc tgcatcgcca ggcacagtgc ggccgtcatc gccgcctgcg gcagctcgcc
     1561 gtagaggcgg agcagcagcg cgccgaggaa cgcgccgatc atgccgccaa gactgaagcc
     1621 gccatggaac atcgacatgg tcggcagccg caaggccttt tccaccgcga tgccgtgggc
     1681 gttcatggcc acatccatgc tgccgatcga catccccatc aggaagcctg ccaccacgaa
     1741 tgtggtcatg tccggcgcga ggatggggcc gaagaaggtg aggcagaaga ggatgccggt
     1801 gatgccgctc atcctggcgc tgccgaatcg gttgatcagc atgccggcgg tgggcatggc
     1861 caccaccgcg ccgccggcaa aggccagcag ggccagcccg aagaccgccg gaccggcatc
     1921 gaggcgttcc ttcgccaggg gaatatgcgg cacccaggat ccgatcagga tgccgtgcag
     1981 caagaacatg gcacccacgg cccagcgggc acggacagcg tcgataaagg tcatggcagg
     2041 tcgcttcgtt tgagggctgt cttcctagcc gattgcaccc ggcgccgcta cagggcattg
     2101 ttgaacgcaa ccggggtgag gttggggaac ggcattccgg atcgagaaga aacgaaacgg
     2161 gaggacccag catgtccaga gacgaggccg caccggccta tgtgccgccg cctgccatca
     2221 agatcaaaga aatcggcgtt gacgacatct gggccgcgct ccgggctgga tggcgcgatt
     2281 tcaccagtgc cccgctgatc ggccttttct tcggggcgat ctatgtcgca ggcggcatcc
     2341 tgattctgct cctgctcagc gtctatcatc agccctggtg gatcattccc attgctgtcg
     2401 gctttccgct tatcgggccc ttcgtggccg tcggactcta tgaggtgagc cgcaggctgg
     2461 cggctgggca gcccatcgtc tggggcgagg tgctttcggt gatctgggcc cagcgttcgc
     2521 gacagatcgg atggatggcc ttcgtcgtgc tcttcatctt ctggatgtgg ctttatgaag
     2581 tgcggatgct gcttgccatc tttctcggct tcaagtcctt ttccagcatt gacgctttcc
     2641 tcaccgttgt gaccacgacg gccgaaggcc tcggcttcct tgccgtcggg acggtggttg
     2701 gcgccttcct gtcattcatc ctgttctgca cgacggtggt tgccataccg ctgctgctcg
     2761 accgcgaaac cgatctgatc accgccatca tcgccagttt ccgctcggtc ttccggaacc
     2821 cggcgccgat gatcggatgg gggatcatcg tcgcaacgct cacggcactg gcattgctgc
     2881 cggtattcct cggcctgctg gtcgtcctgc cggtgctggg ccatgcgacc tggcacctct
     2941 atacccgcgt cgtcgcgccg ccctgatcag actccgacgc ccttggcctg catcgccgcg
     3001 cggatctcgt cgaggatcgc gggatcgtcg atggtggctg gcgtcttgaa cgcctccttg
     3061 tcggcgatct tgcgcatggt gccgcgcagg atcttgccag atcgggtctt gggcaggcgc
     3121 ggcacgacgg ccacggtgcg cagcgcggcc acggcgccga tcttttcgcg caccagcccg
     3181 acacattccg tctcgatgtc ggccgccggc ttcgtgacgc ccgacttgag gaccacgagt
     3241 cccagaggca cctggccctt gagcgcatcg gcgatgccga tgaccgcgca ttccgccacg
     3301 ctgggatggc ccgcgagcac ttcttccatg ccacccgtcg agaggcgatg gcccgccacg
     3361 ttgatgatgt cgtcggtgcg cgccatgatg tagaggtaac cgtcctcgtc catgtagccc
     3421 gcgtcggccg tctcgtaata gccggggaaa tgcgacaggt aggatttccg gaagcgctcg
     3481 tcgttgcccc acagcgtggg caggcaggag ggcggcagcg gcagc
//
LOCUS       DBNGVR010000416         2037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4868067, whole genome shotgun sequence.
ACCESSION   DBNGVR010000416 DBNGVR010000000
VERSION     DBNGVR010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4868067"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="AC16A0_08825"
     CDS             <1..259
                     /locus_tag="AC16A0_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3105479.1"
                     /translation="LRVGRLMDEGRSTPEMVSLVKRNSCGKALEIARMARDMHGGNGI
                     SEESHVIRHMVNLETVNTYEGTHDVHALILGRAQTGIQAFQ"
     gene            256..714
                     /locus_tag="AC16A0_08830"
     CDS             256..714
                     /locus_tag="AC16A0_08830"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3105480.1"
                     /translation="MSDAVIALADPAEPSVRALIEALDAYMIPLYPAESNHLLDIETL
                     RRPEMRFFAARVGAQTLGCGGCWLHDDYAEIKRVYVNPQARGLGLAKKLMDRIEDEAR
                     AAGMTIARLETGIHQPEAIGLYRKLGYALREAFGDYPADDPNSVFMEKRL"
     gene            711..1790
                     /locus_tag="AC16A0_08835"
     CDS             711..1790
                     /locus_tag="AC16A0_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR3105481.1"
                     /translation="MTVAPFPLIEIAGPPFARGESYGAQARKRILRSLALYRGRLEKL
                     GLTSDRIADLVRQTLPEMRRFGEHYLAEMEGIARGAGLALDDIVLVNARTEIVALAKR
                     REKPVDGCTGIVILPSHASGGALIHAQNWDWLAACAETGVVLRIRRDDGPDILTFTEA
                     GGLARSGLNAAGLAITAKYLESDRDYRQAGVPLPLIRRRVLEQQHLALAIEAIAATPK
                     ACSNNMILSDAKGFAIDLECAPDEVFPLHAANGRIVHANHWQSPVALSKLKDTGLAAV
                     PDSLYRDRRVIELLPDRPDTEDVKRALFDDFGAPYAVCRPSIDEGDGNLGATVAMIVM
                     VPARGIMEICAMPALSRSFERYSLE"
     gene            complement(1779..>2037)
                     /locus_tag="AC16A0_08840"
     CDS             complement(1779..>2037)
                     /locus_tag="AC16A0_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016554296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR3105482.1"
                     /translation="SAHTRLLNFTSNIASLAIFAASGKIVWILGLAMGVGQFLGAQVG
                     ARLAISQGARIIRPLLVLVSCGMAIKLLLDPANPITHYLFE"
BASE COUNT          359 a          675 c          675 g          328 t
ORIGIN      
        1 cctgcgagtg ggccggctga tggacgaggg ccggtccacc cctgagatgg tctccctggt
       61 gaagcgcaac tcctgcggga aggccctgga gatcgcgcgt atggcccgcg acatgcacgg
      121 cggcaacggc atctcggaag agtctcacgt catccgccac atggtgaacc tggagacggt
      181 gaacacctac gaaggcacgc atgacgtgca tgcgctgatc ctgggccgcg cccagaccgg
      241 gattcaggcc ttccagtgag cgacgccgtc atcgcgcttg cggacccggc ggagccatcc
      301 gtccgtgcac tgatcgaggc gctcgacgcc tacatgatcc cgctctatcc ggcggagagc
      361 aatcatctgc tcgacatcga gacgctgcgc cggcccgaga tgcgcttctt cgccgcccgc
      421 gtcggcgcgc agacgctggg gtgcggcggc tgctggctgc atgacgatta tgccgagatc
      481 aagcgcgtct atgtgaaccc gcaggcgcgc ggcctgggcc ttgcgaagaa actcatggac
      541 cggatcgagg acgaggcccg cgcggccggc atgacgattg ccaggctcga gaccggcatc
      601 caccaaccgg aggccatcgg cctctaccgc aagctgggct atgcgctgcg cgaggccttc
      661 ggcgactacc cggcggatga tcccaacagc gtcttcatgg agaagcgctt gtgacggtgg
      721 cacccttccc gctgatcgag atcgcgggac cgccctttgc gcggggcgag agttacggtg
      781 cgcaggcacg caagcggatt ctccgcagcc tggcgctcta tcgcggcagg ctggagaagc
      841 tgggactgac ttccgatcgc atcgccgatc tcgtccgcca gaccctgccg gagatgcgcc
      901 gcttcggcga acactacctt gccgaaatgg aaggcatcgc gaggggagca ggccttgcgc
      961 tcgacgacat tgttctggtg aacgcgcgca ccgagatcgt ggcccttgca aaacgccggg
     1021 agaagccggt cgacggctgc accggcatcg tgatcctgcc gtcgcacgcg tccggcggtg
     1081 cactcatcca tgcgcagaac tgggactggc tggccgcctg cgccgagacc ggcgtggtgc
     1141 tgcgcatacg gcgcgacgac ggaccggaca tcctcacctt cacggaagcc ggaggtctgg
     1201 cgcgttcggg tctcaatgcc gcaggcctcg ccatcacggc caagtacctc gagagcgacc
     1261 gggactaccg tcaggcgggc gttcccctcc cgctcatccg gcggcgcgtg ctggagcagc
     1321 agcaccttgc gcttgcgatc gaggccatcg ccgccacgcc caaggcctgt tccaacaata
     1381 tgatcctgtc ggacgccaaa ggcttcgcca tcgacctcga gtgtgcgccc gacgaggtct
     1441 ttccgctgca tgccgcgaac gggcgcatcg tccatgccaa ccattggcag agtcccgtcg
     1501 ccctttcgaa gctgaaggac accggcctcg ccgccgttcc cgacagcctg taccgagacc
     1561 ggcgggtgat cgaactcctc cccgatcgtc cggatacgga ggatgtgaag cgcgcgctgt
     1621 tcgacgactt cggcgcgccc tacgccgtct gccgcccgtc catcgacgag ggcgatggca
     1681 atctcggagc gaccgtggcg atgatcgtga tggtcccggc ccgcggcatc atggagatct
     1741 gcgccatgcc ggcgctgagc cggagctttg agcggtattc actcgaatag gtagtgggtg
     1801 atcgggttgg cgggatcgag cagcagcttg atcgccatcc cgcaggatac cagcaccagc
     1861 agcggccgga tgatccgggc accttgcgat atggctagcc gcgcaccgac ctgcgcgccg
     1921 aggaactgcc ccacgcccat ggcaagaccc aggatccaga cgatcttgcc ggaggctgcg
     1981 aagatcgcca gggaggcgat gttggaagtg aagttgagca ggcgcgtgtg ggcagat
//
LOCUS       DBNGVR010000417         5368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_349319, whole genome shotgun sequence.
ACCESSION   DBNGVR010000417 DBNGVR010000000
VERSION     DBNGVR010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_349319"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1931)
                     /locus_tag="AC16A0_08845"
     CDS             complement(21..1931)
                     /locus_tag="AC16A0_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3105483.1"
                     /translation="MSVRRAMDEALRLYNQGKIPAAAKLAAQIVAARPRLAEAHNLMG
                     VIMLAQGKTAEAIKAIGRATRLDPQNAQYFSNLGELERQRGKLQEAGIALRAAIEINP
                     QHPQALNNLGILHYDRREFDEAVACYEKALAVSADYAEAHNNLGNALRALGRFDAAID
                     HYQSAVLIRENYPEAYNNMASVLRDQGHVAEAEHAYRKAISLRRDYIEAYVNLAAMLD
                     GDDHGDEALRVLGEALKINARHTPTLIQIARTQLRKMNYEQAEQAARLALQNEPNSAD
                     AHAVLAQILHETDRLHDAIDAFEATLRLKPSLIEANSMYGVCLKSVGRLDEARAQFIR
                     TLELAPAALGVYANLADLDKFTPDNPQLKAMEKIFAEAPDPASDRYLPLHFAIGKAYD
                     DIGDYGKAFRHFKTGADLKRAKLSYDEAQTFGFFDSIREVFNADYFASRPFEGRSTPV
                     PVFIIGMPRSGSTLVEQVLSNHPDTFGAGEIKEFSRQLNALRGRFPGLPKYPQIVQRM
                     NAAQYALVADGYLSKVTALAPKAARITDKLLTNYYFVGLLHTLFPNARFIHTRRNPVD
                     TWPRCARLHRLAQEAQGFCRGLGIRKQVPRDVNPKSGWPELFERSFYCCDRFNRRGSR
                     MKNRTQWPPERI"
     gene            complement(2181..2669)
                     /locus_tag="AC16A0_08850"
     CDS             complement(2181..2669)
                     /locus_tag="AC16A0_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HIR3105484.1"
                     /translation="MKRTASCLLGAAALAATLAMPLAGAQANTTVRAGTLQCDVSGGI
                     GLVFVEKQSMTCTFTDPNGNLIGNYTGQIKEVGVALGATTEGVLVWAVLAAQHSIPAG
                     ALAGTYAGVTANASFGVGAGANVLVGGTGRSFVLQPLSVEGQVGVNIAGGVTTVTLEA
                     AP"
     gene            complement(2753..3694)
                     /locus_tag="AC16A0_08855"
     CDS             complement(2753..3694)
                     /locus_tag="AC16A0_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105485.1"
                     /translation="MEAIEILFTASLWAAAIRIASPLIFGTLGELICERAGVLNLGIE
                     GIMTAGAFSGWLAVYMGADLWFGVAVAAIVGATLGLLHGVLVVPLGLSQHVTGIGVTL
                     LATSLCYYFYRLAFPEVTSPPKIDPFQPWPVPVLSEIPVLGPALFQQTPLTYLAFVMV
                     VLVAWFLERTPLGLAVRTVGENPAAAEAQGINVTWVRIGAVMAGSALMGVGGSFITLS
                     AFNSFFFDMMNGRGWICIALVVFGAWRPGKALLGAVLFGAFDAYQLRLQQLAGAVVPY
                     QLFLMLPFVLSILALIFMSRRVAYPRALMVPYRKGER"
     gene            complement(3697..4746)
                     /locus_tag="AC16A0_08860"
     CDS             complement(3697..4746)
                     /locus_tag="AC16A0_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105486.1"
                     /translation="MRLEPRQSRPLLLVLLLPALALVTAGVFCALLVMLSGASPFQVF
                     GLMIKGALGSQFAFLETLTRATPLIFTGLAVAVAFRAKLWNIGAEAQLYIGAVMTVVL
                     GTGLLPLPWPVLIPVLMFSAALGGALVLAVPTFLKTRFGVDEVVTTLLLNFIVLLFVS
                     MLLEGPLKDPMGLGWPQSKKVVTDAQLPKLVQGKRLHAGFVIAILAAMLVWVFDRRAA
                     LGYEMRAVGYNSKAAAFAGIPVNPVLAKTALLSGGLAGLAGWSEVAGLKGNLTLDLSP
                     GFGYTGIVVAMLALLHPLGVVVAAIFVAAVFVGADAMSRSAGVPSYIAHVMVALSLLC
                     MVTAIMFTRYRVRWT"
     gene            complement(4736..>5368)
                     /locus_tag="AC16A0_08865"
     CDS             complement(4736..>5368)
                     /locus_tag="AC16A0_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3105487.1"
                     /translation="VISGMQQPSHGTLELNGTTLHAVTPRAMVANGVARIPEDRHAEG
                     LIGDMTITENVVSETYRSPALSRFGLIDWRKARTRAEDIIGAYEVKCPSPDVPVRLLS
                     GGNMQKLILGRVMAERPKLVLANQPTRGLDVGAVSYVHARLLEARSKGAAILLLSEDL
                     DELLSLSDRIAVIYRGRLSPLRDRKGTTIADLGLLMAGHGFAGGEDSHAA"
BASE COUNT         1015 a         1716 c         1771 g          866 t
ORIGIN      
        1 ggagagcgac tgactggctt tcagatacgt tctggaggcc actgagtccg attcttcatt
       61 cggctccctc gccgattgaa tcggtcacag caatagaacg atcgttcaaa caattccggc
      121 cagccgcttt tggggttgac gtcgcggggc acttgcttcc gaattcctaa gccgcgacag
      181 aagccttgcg cttcctgtgc caaccgatga agccgagcgc accgaggcca ggtgtcgacc
      241 ggattgcgcc tggtatggat gaagcgggca ttcggaaaca gcgtgtgcag cagcccgacg
      301 aaatagtagt tcgtcagcag cttgtcggtg atgcgcgccg cctttggcgc cagcgccgtc
      361 accttcgaca gatagccatc ggcgaccagc gcatattgtg cggcattcat gcgctgcacg
      421 atctgcggat acttgggcag gccgggaaag cggccgcgca gcgcgttgag ctggcgcgag
      481 aactccttga tctcccctgc cccgaaggta tcgggatgat tggagaggac ctgttcgacc
      541 agggtcgagc ccgagcgcgg catgccgatg atgaagacgg gcaccggcgt gctgcggccc
      601 tcgaagggcc ggctggcgaa atagtccgcg ttgaacactt cgcgaatcga atcgaagaag
      661 ccgaaggtct gggcctcgtc ataggagagt ttggcccgct tgagatcggc gccggtcttg
      721 aaatgccgga aggccttgcc gtagtcgccg atgtcgtcat aggccttgcc gatggcgaag
      781 tgcagcggca gataacggtc gctcgccgga tccggcgcct cggcaaagat cttttccatc
      841 gccttcagct gcgggttgtc cggcgtgaac ttgtcgaggt cggcgagatt ggcatagacg
      901 ccgagggccg ccggcgccag ctcgagcgtc ctgatgaatt gcgcccgcgc ttcgtcgagc
      961 cgccccaccg acttcaggca tacgccatac atggaattgg cttcgatcag cgagggcttc
     1021 aggcgcagcg tggcctcgaa cgcatcgatg gcgtcatgga gccggtccgt ctcgtgcagg
     1081 atctgcgcca gcacggcatg ggcgtcggcc gagttgggct cgttctgcag ggcaaggcgc
     1141 gcggcctgct cggcctgttc gtagttcatc ttgcgcagct gcgtccgtgc gatctggatc
     1201 agagtcggcg tgtgcctggc attgatcttg agcgcctcgc caagcacgcg gagcgcttcg
     1261 tcgccgtggt cgtcgccatc cagcatcgct gcgaggttga cataggcctc gatgtagtcc
     1321 cgccgcaggc tgatggcctt gcgataggca tgctcggcct cggcgacatg gccttggtcg
     1381 cgcagcaccg aggccatgtt gttgtaagcc tccggataat tctcgcggat cagaaccgcc
     1441 gactgatagt gatcgatggc cgcgtcgaac cggccgaggg cgcgcagcgc attgcccagg
     1501 ttgttatggg cctcggcata gtcggcgctg accgccaagg ccttctcgta acaggcgaca
     1561 gcctcgtcga attcgcggcg gtcgtaatgc aggatcccca gattgttcag ggcctgcgga
     1621 tgctgcggat tgatctcgat cgcggcacga agcgcgatgc cggcctcctg cagcttgccg
     1681 cgctgccgct ccagctcgcc caggttggag aagtattgcg cattctgcgg atcgaggcgg
     1741 gtggcgcggc cgatcgcctt gatcgcctcc gcggtcttgc cctgggcgag catgatcacg
     1801 cccatgagat tgtgcgcctc ggccagccgc ggccgcgctg cgacgatctg ggcggccagc
     1861 ttcgcggcgg ccggtatctt cccctggttg tagaggcgca gcgcctcgtc catcgcccgc
     1921 ctgacggaca tgaccttctt gtcagcaccc gtccctgtct ccgacggagg aacagacctt
     1981 tgttcagcca cttctcacct ctggtcgggc acgagggtca ccggcatcga gaatcgacat
     2041 ccggaatcga ccgccgcgcg gcagcctagt ttcagccgtc cggcaaggca attgccaagg
     2101 cctggcctcg attgacgcga agcggccgcc ccggtccgtc cggggaggcc tgagcccaag
     2161 gttggggctc tgtccgccga tcagggtgcg gcttccagcg tcacggtggt gacgccaccc
     2221 gcgatgttca cgccaacctg gccctcgacc gacagaggtt gcagaacgaa ggaccgcccg
     2281 gtgccgccca ccagcacatt ggcacccgcg cccaccccaa aggaggcatt ggcggtgacc
     2341 ccggcatagg ttccggcgag ggcaccggcc ggaatgctgt gctgggcggc cagcacggcc
     2401 cagacgagaa cgccttcggt ggtcgcgccg agcgccacgc ccacttcctt gatctgaccg
     2461 gtgtaattcc cgatcagatt gccattcgga tcggtgaagg tgcaggtcat ggactgcttc
     2521 tcgacgaaga cgaggccgat gccgcccgac acgtcgcatt gcagggtacc ggccctgacg
     2581 gtcgtattgg cctgtgcgcc cgccaggggc atggccaacg tggcggcaag ggcggctgcg
     2641 ccaagaagac acgaagcggt ccgtttcatc tgatcctcca agatctcatc atttcaaggg
     2701 aagggggcgt gattcgcttt tgccacagcg gccgggccgc ttgcaaatga agtcagcgct
     2761 cgccctttcg atagggaacc atgagggcgc gggggtaggc cacccggcgc gacatgaaga
     2821 tcagcgcaag gatcgacagc acgaagggca gcatcaggaa gagctggtaa ggcaccaccg
     2881 ctccggccag ctgctgcagg cgcaactgat aggcatcgaa agccccgaaa aggaccgcgc
     2941 cgagcaaggc cttgcctggc cgccaggcgc cgaagaccac cagggcgatg cagatccagc
     3001 cgcgcccgtt catcatgtcg aagaagaagg agttgaacgc cgacaatgtg atgaagcttc
     3061 cgcccacacc catcagcgcg ctgccggcca tgacggcgcc gatgcgcacc caggtcacgt
     3121 tgatgccctg agcctcggcg gcggccggat tctcgccgac ggtgcggacc gcgagtccca
     3181 gcggcgtacg ttccaggaac cacgcgacca gcaccaccat cacgaaggcg aggtaggtga
     3241 gcggcgtctg ctggaacagt gccggaccga gaaccgggat ctcggacagg accggcacgg
     3301 gccagggctg gaagggatcg atcttcggcg gcgacgtcac ttccgggaag gcgaggcggt
     3361 agaagtagta gcagagcgac gtggcgagca gcgtcacgcc gatgccggtg acatgctgcg
     3421 acagtccgag cggcacgacc agcaccccat gcagaagtcc cagcgttgca ccgacgattg
     3481 ccgccaccgc cacgccgaac cacaggtctg ctcccatgta gaccgccagc cagccggaaa
     3541 aggcgccggc cgtcatgatg ccttcgatgc caagattgag cacgccggcg cgctcgcaga
     3601 tcagctcgcc cagcgtgccg aagatcaagg gcgaggcaat ccggatggcg gcggcccaga
     3661 gcgaagcggt gaagaggatc tcgatggctt ccatcttcag gtccagcgta cacggtaacg
     3721 ggtgaacatg atggcggtca ccatgcagag cagcgacagc gccaccatga catgggcaat
     3781 gtagcttggc acgccggcgc tgcggctcat ggcatcggca ccgacgaaga ccgcggcaac
     3841 gaagatggcc gccaccacaa cacccagcgg atgcagcagc gccagcatgg ccaccacgat
     3901 gccggtgtag ccgaagcccg gtgacagatc gagcgtgaga ttgcccttga ggcccgccac
     3961 ttccgaccag ccggccaggc cagcgagacc gccagacagc agggcggtct ttgccagtac
     4021 gggattcacg gggattccgg caaaggccgc ggccttggaa ttgtagccca cggcgcgcat
     4081 ctcgtaaccc agtgccgcgc gccggtcgaa gacccagacc agcatggcgg cgaggatggc
     4141 gatgacgaag cccgcatgca ggcgcttgcc ctggaccagc ttgggcagct gcgcatcggt
     4201 gaccaccttt ttcgactgcg gccagccgag gcccatggga tccttgagcg gcccttccag
     4261 cagcatgctg acgaagagca gcacgatgaa gttcaagagc agcgtggtca cgacctcgtc
     4321 cacgccgaag cgcgtcttga ggaatgtcgg cacggccagc accagagcgc cgcccagcgc
     4381 cgccgaaaac atcagcacgg gaatcagaac cggccacggc aagggcagga ggccggtccc
     4441 cagcaccacg gtcatcaccg cgccgatgta gagctgtgcc tcggcgccga tgttccacag
     4501 cttcgcccgg aaggccaccg ccacggcgag acccgtgaag atcaggggcg tggcgcgggt
     4561 cagggtttcg agaaaggcga actggctgcc aagtgcgccc ttgatcatca ggccgaagac
     4621 ctggaagggc gaagcgcccg acagcatcac cagaagggca cagaagacgc ccgcagtcac
     4681 cagcgccagc gcggggagca gaaggaccag gagcaagggc cgggactggc ggggctcaag
     4741 ccgcatggct gtcctcgcct ccggcgaaac catgccccgc catgaggagg cccagatcgg
     4801 cgatggtggt tcccttgcgg tcgcgaagcg gcgagagccg gccgcgatag atcaccgcga
     4861 tccggtcgga cagcgaaagc agttcatcga gatcttccga gagcagcagg atcgcagcgc
     4921 ccttggatcg cgcctccagc aatcgggcat gcacgtaact gacggcaccc acgtccagtc
     4981 cgcgtgtcgg ctgattggcc aggacgagct tcggcctttc ggccatgacc cggcccagga
     5041 tcagcttctg catgttgccg ccggacagca ggcgcaccgg cacatcgggc gacgggcact
     5101 tcacctcata ggcgccgatg atgtcctcgg cccgcgtcct cgccttgcgc cagtcgatga
     5161 ggccgaagcg cgacagggcc ggactgcgat aggtttccga cacgacattc tcggtgatgg
     5221 tcatgtcgcc gatcaggcct tcggcatggc ggtcttcggg aatgcgcgcc acgccgttcg
     5281 ccaccatggc gcggggggtg accgcgtgaa gcgtcgtgcc attcagttcc agcgtgccat
     5341 ggctgggctg ctgcatcccg gagatgac
//
LOCUS       DBNGVR010000418         2144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2117713, whole genome shotgun sequence.
ACCESSION   DBNGVR010000418 DBNGVR010000000
VERSION     DBNGVR010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2117713"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /gene="ychF"
                     /locus_tag="AC16A0_08870"
     CDS             complement(<1..1031)
                     /gene="ychF"
                     /locus_tag="AC16A0_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR3105488.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAQAANYPFCTIEPNVGD
                     VAVPDPRLDVLAKIAGSGQIIPTRLTFVDIAGLVRGASKGEGLGNQFLANIREVDAIA
                     PVVRCFEDGDITHVEGKIDPIADIETIETELMLADLDSLEKRIVNLEKKAKTGDKEAK
                     ELADLINRALVLLREGKPARLVQRTPEEEKAFRGLGLLSSKPVLYVCNVDEGSADKGN
                     DFSARVFGRAAEEGAVAVVVSAKIESEIAVLPEADQKDYLEAVGLHEPGLNRVIRAGY
                     DLLHLVTYFTVGPKEARAWTITVGTRAPAAAGVIHTDFEKGFIRAETIAYDDYVSGKG
                     EAGAREAGK"
     gene            complement(1083..1682)
                     /gene="pth"
                     /locus_tag="AC16A0_08875"
     CDS             complement(1083..1682)
                     /gene="pth"
                     /locus_tag="AC16A0_08875"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3105489.1"
                     /translation="MHLLVGLGNPGPKYAGHRHNVGFMAVDEIVRRHGFSAWRKRFQG
                     EISEGTFGGEKVLVLKPMTYMNESGRAVGEAMRFYDIDPSQVIVFYDELDLEPGKLRV
                     KFGGGAAGHNGIRSITSHAGPHFQRVRIGIGHPGDRNVVMPHVLSDFAKADKTWLVPL
                     LDAVADHAGLLVADQAASFQNKVHLALNPAPAKTPKPEN"
     gene            complement(1707..>2144)
                     /locus_tag="AC16A0_08880"
     CDS             complement(1707..>2144)
                     /locus_tag="AC16A0_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR3105490.1"
                     /translation="HVDFLRLGKGARFVVDVPVTFKNQESAPGVKTGGVLNIVSHDVA
                     LYCTPELIPDAIVVDCGALQIGQSIHLSEIELPKGVTSAARDDVTLCTVTAQAKEEEV
                     AAPVAAEVPATAQKAPAAGAAPAAAGKDAKAPAAAAAPAAKKK"
BASE COUNT          347 a          706 c          697 g          394 t
ORIGIN      
        1 aacttgccgg cctcgcgcgc accggcctcg cccttgccgg agacgtagtc gtcataggca
       61 atggtttcgg cgcggatgaa gcccttttcg aaatcggtat gtatcacgcc cgccgcagcg
      121 ggtgccctgg tgccaaccgt gatggtccag gcgcgggctt ccttggggcc gaccgtgaag
      181 taggtcacca gatgaagcaa gtcgtagccg gcgcggatca cgcggttgag tccgggctca
      241 tgtaggccga cggcctccag atagtccttc tgatcggcct cgggcagcac cgcgatctcg
      301 ctttcgatct tggcggagac aaccaccgcg acggcgccct cctccgccgc acggccgaag
      361 acgcgggccg agaagtcatt gcccttgtcg gcagagccct cgtcgacgtt gcagacatag
      421 agcacgggct tcgacgacag taggcccaga ccgcggaagg ccttctcttc ctccggcgtg
      481 cgctggacga ggcgcgcggg cttgccctcg cgcagcagta ccagcgcgcg gttgatgagg
      541 tcagccagtt ccttggcctc cttgtcgccg gtcttggcct tcttctccag attgacgatg
      601 cgcttctcga gggagtcgag gtcggccagc atcagctcgg tctcgatcgt ctcgatgtcg
      661 gctatcggat cgatcttgcc ttcgacgtgg gtgatgtcgc cgtcctcgaa gcagcgcacc
      721 acgggggcga tggcatcgac ctcgcggatg ttggcgagga actggttgcc gaggccctcg
      781 ccctttgacg cgccgcgcac caggccggcg atatcgacga aggtcaggcg cgtcgggatg
      841 atctggccgc tgcccgcgat cttagccaat acgtcgaggc gcggatcggg tacggccacg
      901 tcaccgacat tgggctcgat ggtgcagaag ggataattcg ccgcctgggc cgccgcggtc
      961 tgcgtcagcg cattgaagag cgtcgacttg ccgacattgg gaagtccgac gatgccacac
     1021 ttgaaaccca tctgttccgt ctctcttgca agtcccgcga cagcgcctgg cgcgggagga
     1081 attcagtttt ccggcttggg cgtcttcgcc ggcgcgggat tgagggcgag gtgcaccttg
     1141 ttctggaacg aggccgcctg gtccgccacc agcaacccgg catgatcggc cactgcatcg
     1201 agaagcggca caagccaggt cttgtcggcc ttggcgaagt cgctcagcac atgcggcatg
     1261 accacgttgc ggtcgcctgg atggccgatg ccgatccgca cccgctggaa gtgcgggccg
     1321 gcatgcgacg tgatcgaacg gatgccgttg tggcctgcgg cgccgccgcc gaacttcacc
     1381 cggagcttgc ccggttcgag atcgagctcg tcgtagaaga cgatcacctg cgacggatcg
     1441 atgtcgtaga agcgcatcgc ctcgccgacg gcacggccgc tctcgttcat gtaggtcatg
     1501 ggcttgagca caagaacctt ctcgccgccg aacgttccct cggagatttc accctggaag
     1561 cgcttgcgcc acgccgaaaa gccatggcgg cggacgatct cgtccaccgc catgaagccg
     1621 acattgtgcc ggtgcccggc atacttgggt cccggatttc cgaggcccac gagaaggtgc
     1681 attgccttct ccccgcgtgg cgtccgttac ttcttcttgg cagccggcgc cgcagcggcg
     1741 gccggcgcct tggcgtcctt gccggcagca gccggagccg ctcccgcagc cggcgccttc
     1801 tgggccgtgg ccggaacttc ggcagcgacc ggggcggcca cttcctcttc cttggcctga
     1861 gccgtcacgg tgcagagcgt cacgtcgtca cgtgcggccg aggtcacgcc cttgggcagc
     1921 tcgatctcgg acaagtggat cgactggccg atctgcagcg cgccgcagtc cacaacgatc
     1981 gcgtcgggaa tgagttccgg cgtgcagtaa agcgcaacat cgtgcgacac gatgttcagc
     2041 acgccgccgg tcttcacgcc cggagccgac tcctggttct tgaaggtcac cggcacgtcg
     2101 accacgaagc gcgcgccctt gccgagccgc aggaaatcga catg
//
LOCUS       DBNGVR010000419         1523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4475201, whole genome shotgun sequence.
ACCESSION   DBNGVR010000419 DBNGVR010000000
VERSION     DBNGVR010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4475201"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16A0_08885"
     CDS             complement(<1..296)
                     /locus_tag="AC16A0_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HIR3105491.1"
                     /translation="MAKQKIKKVVLAYSGGLDTSIILKWLQTEYGAEVVTFTADLGQG
                     GELEPARKKAEMLGIKEIYVEDLREEFIRDFVFPMFRANAVYEGVYLLGTSIARP"
     gene            complement(437..841)
                     /locus_tag="AC16A0_08890"
     CDS             complement(437..841)
                     /locus_tag="AC16A0_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105492.1"
                     /translation="MRQAAKLALGLLLIAGTAFGMAALVVWYFAIAAQRAGQVYGIFA
                     AAALGMILLCLLGERLHIRALAYYLVAGAVPFLGADVLLSLRFDIMMGPKGYQDGLVP
                     AAIVGLFLGGFYGIVTGRDAGGHQPPPDPPWR"
     gene            complement(841..>1523)
                     /gene="rlmN"
                     /locus_tag="AC16A0_08895"
     CDS             complement(841..>1523)
                     /gene="rlmN"
                     /locus_tag="AC16A0_08895"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HIR3105493.1"
                     /translation="IRDWPGDIEANPSAPLGGRFITNIVLMGMGEPLYNFDNVKDAME
                     IVSDGEGISLSKRRITLSTSGVVPEIPRAGEEIGTMLAISLHGTTDEIRDKLVPLNRK
                     YPIKELLQACRNYPGLSNARRITFEYVMLKGVNDTLADAKRLVQLLKGIPAKVNLIPF
                     NPWPGTQYECSDWDTIETFAEVLNRAGYASPVRTPRGRDIMAACGQLKSESQKLRAKE
                     RMALDAGA"
BASE COUNT          261 a          468 c          530 g          264 t
ORIGIN      
        1 ggccgggcga tcgaggtgcc gagcaggtag acgccctcat agaccgcatt ggcgcggaac
       61 atggggaaga cgaagtcgcg aatgaactcc tcacgcaggt cctcgacgta gatttccttg
      121 atgcccagca tctcggcctt cttgcgcgcc ggttccagct cgccgccctg gcccaggtcg
      181 gcggtgaagg tcacgacctc ggcgccatac tcggtctgca gccacttcag gatgatggag
      241 gtatcgaggc cacccgaata ggcgaggacg actttcttga tcttctgctt ggccatcggc
      301 ggaccccttc tcaaacgaag ccccggcgtg gcgccggggt ggactgggcg ggccgttcga
      361 atgatctgtt ggcggcccgt cgtgatttca tgcgcggcgt atcatgccca agcggcgttc
      421 cgcaagggcg ggaaccttag cgccaaggcg gatccggcgg gggctggtgg ccgccggcgt
      481 cgcggccggt gacgatgccg tagaacccgc cgaggaagag gccgacaatg gcggcaggga
      541 cgaggccgtc ctgataaccc ttgggcccca tcatgatgtc gaagcgcagg ctgagcagga
      601 catcggcgcc gagaaagggt acggcccccg ccacgagata gtaggcgagc gcgcggatgt
      661 gcaggcgctc gccgaggaga cagaggagga tcatgccaag ggccgctgcc gcgaagatcc
      721 cgtagacctg tccggcgcgc tgggcggcga tggcaaagta ccaaacgacg agcgctgcca
      781 tgccgaaggc cgtgccggca atgagcagga ggccgagggc cagtttcgcc gcctggcgca
      841 tcaggcgccg gcgtcgaggg ccatccgctc ctttgcccgc agcttctggc tttcgctctt
      901 caactgtccg caggccgcca tgatgtcgcg gccgcgcggc gtcctgaccg gcgaggcata
      961 accggcgcgg ttgagcacct cggcgaaggt ctcgatcgtg tcccagtcgc tgcactcgta
     1021 ttgcgtgccg ggccaggggt tgaagggaat gagattgacc ttggcgggga tgcccttgag
     1081 cagctgcacc agccgcttgg cgtctgccag cgtgtcgttg acgcccttca gcatcacata
     1141 ttcgaaggtg atgcgccggg cgttcgacag gccaggatag ttgcggcagg cctgcagcag
     1201 ctccttgatc ggatacttcc tgttgagcgg aaccagcttg tcgcggatct cgtcggtggt
     1261 gccgtgcagc gagatggcca gcatggtgcc gatttcttcg ccggcccgcg ggatctccgg
     1321 cacgacgccc gaggtcgaga gcgtgatacg gcgcttggac agcgagatgc cttcgccgtc
     1381 ggagacgatc tccatcgcgt ccttcacgtt gtcgaaattg tagagcggct cgcccatgcc
     1441 catgagcacg atgttggtga tgaaacggcc gcccagcggc gcagacggat tggcctcgat
     1501 gtcgccgggc cagtcgcgga tgc
//
LOCUS       DBNGVR010000420         4316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1726045, whole genome shotgun sequence.
ACCESSION   DBNGVR010000420 DBNGVR010000000
VERSION     DBNGVR010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1726045"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="AC16A0_08900"
     CDS             complement(<1..478)
                     /locus_tag="AC16A0_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3105494.1"
                     /translation="MNKTVNNRAWWLVLPVFAMVAFNALIPLMTVVNYSVQETFGNTE
                     FFWSGTTWFKQLLHDDQFIDSLQRQIIFTATVLVIQIPLGLVIALCMPRSGPWVSVCL
                     VLMSLPLLVSWNVVGSMWNIMALPDIGLLGKAINAMGIPYNYTRQPISAWFTVVLMD"
     gene            complement(475..1548)
                     /locus_tag="AC16A0_08905"
     CDS             complement(475..1548)
                     /locus_tag="AC16A0_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105495.1"
                     /translation="MAEIRIENLRHSYLANPGADEDYALKRLSLTWQDGGAYALLGPS
                     GCGKTTLLNLISGLLTPTEGKILFNGRDVSHASPEERNIAQVFQFPVIYDTMTVYDNL
                     AFPLRNRQVPEAEIKSRVTEIAEMLELSSTLSNRASGLSADGKQKISLGRGLVRADVN
                     VIMFDEPLTVIDPHLKWKLRSKLKELHQRTRRTMIYVTHDQTEALTFADQVVVMNLGE
                     VVQSGTPVELFEKPQHTFVGHFIGSPGMNVRPCEVKGGKAHFAGKTIAVDNGAAATGK
                     GRTEIGVRPEFVSISRGGLPAEVTRVSDAGRFRIVETRCGGHLVKLLVMEGDMVPEGH
                     THLAFDPAHTQIYEDGWIAGAKP"
     gene            complement(1548..2612)
                     /locus_tag="AC16A0_08910"
     CDS             complement(1548..2612)
                     /locus_tag="AC16A0_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105496.1"
                     /translation="MSLELIDITKRVGAETYIHPTSLVLPEGSFNVLLGTTLSGKTTL
                     MQLMAGIQSPSSGTIRFRGQDVTRFPVQRRNVSMVYQQFINYPNFTVYDNIASPLKVA
                     GMSAAEIDRRVGAAAELLRLKSMLKRRPSELSGGQQQRTAIARAIVKDSDLILMDEPL
                     ANLDYKLREELRDELPKLFAGRNAIVVYATTEPSEALLFGGNTATLHEGRVTQFGPTS
                     ATYRAPRDLTTAQVFSDPPINIADVEKRGSKLTVFGDQSWPAGREAENLPDGRYIFGV
                     RPHYVTPLPVKAKTGEIEGRVLVTELSGSESVIHLSVAGKTWVSQSHGIHPFEVGSQA
                     RLHVEVDRGLFFLPSGELVS"
     gene            complement(2710..>4316)
                     /locus_tag="AC16A0_08915"
     CDS             complement(2710..>4316)
                     /locus_tag="AC16A0_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105497.1"
                     /translation="QQQAEMEWFIEASKPFAGMEINVLSEGIPTHSYESTVLTKAFEE
                     ITGIKVNHQILGEGEVVQAVQTQMQTNRNLYDAYVNDSDLIGTHSRLQQAVNLTDFMA
                     GAGKDVTNPGLDLNDFMGIKFTTGPDGKLYQLPDQQFANLYWFRKDWFDRQDLKDKFK
                     AKYGYELGVPVNWSAYEDIAQFFTEDVKEIDGVKIYGHMDYGKRAPDLGWRMTDAWLS
                     MAGAGTVGEPNGIPIDEWGIRMEKGSCNPSGASVARGGETNGPASVYAIAKWDEWLRK
                     YAPPGAADYDFYQSLPALAQGNVAQQIFWYTAFTASMVAPKSEGNNTVDDQGNPLWRM
                     APSPHGPYWKDGQKVGYQDVGSWTILKSTPEDRAKAAWLYAQFVVSKTVDVKKSQVGL
                     TFIRDSTVRHDSFTERAPKLGGLVEFYRSPDRVRWSPTGINVPDYPKLAQLWWQQIGD
                     VNSGAFTPQEAMDRLASEMEQVMERMEAADKANNTYGGCGPRLAEPKDPSEWLGKPDG
                     PKAKLENEKEPGQTIAYEEIVKRWQE"
BASE COUNT          794 a         1276 c         1380 g          866 t
ORIGIN      
        1 catccatcag caccacggtg aaccaggcgg agatgggctg gcgggtatag ttgtagggaa
       61 tgcccatcgc attgatcgcc ttgccgagca ggccgatgtc gggcagcgcc atgatgttcc
      121 acatcgagcc caccacgttc cacgacacca gcaggggcag ggacatcagc accaggcaga
      181 ccgacaccca agggccgctg cgcggcatgc agagcgcaat gacaagcccg agcggaatct
      241 ggatcaccag cacggtcgcg gtgaagatga tctggcgctg cagtgaatcg atgaactggt
      301 cgtcatgcag cagctgcttg aaccacgtcg tgccggacca gaagaactcg gtgttgccaa
      361 aggtctcctg gaccgagtag ttcaccaccg tcatcaacgg aatgagggcg ttgaaggcca
      421 ccatggcgaa gacgggcagg accagccacc aggcgcggtt gttcaccgtc ttgttcatgg
      481 tttcgctccc gcgatccagc catcttcata gatctgcgtg tgcgcaggat cgaaggcgag
      541 atgcgtgtgg ccttcgggta ccatgtcgcc ctccatgacg aggagcttca ccaggtggcc
      601 gccgcagcgg gtctcgacga tgcggaagcg gccggcatcc gacacgcgtg tgacctccgc
      661 cgggaggccg ccccgggaga tcgagacgaa ctcagggcgc acgccaatct cggtgcggcc
      721 cttgcctgtc gccgccgcgc cgttgtctac cgcgatggtc ttgccggcaa agtgggcctt
      781 gccgcctttc acctcgcagg gacgaacgtt catacccgga gagccgatga agtggccgac
      841 aaaggtgtgc tgcggctttt cgaagagttc cactggcgtc cccgactgca cgacctcgcc
      901 cagattcatc accacgacct gatcagcgaa tgtcagggcc tcggtctggt catgggtgac
      961 ataaatcatg gtccgccgcg tacgctggtg aagttccttc agcttggacc ggagcttcca
     1021 cttcaagtga ggatcgatga ccgtcaacgg ctcgtcgaac atgatgacat tgacgtcggc
     1081 acgcaccaga ccgcgaccca gcgagatctt ctgcttcccg tcggccgaga gtccggaggc
     1141 gcgattggac agggtgctgg acagttccaa catctccgcg atttcggtca cgcgcgactt
     1201 gatttcggcc tccggcacct gtcggttgcg caacgggaag gcgaggttat cgtagaccgt
     1261 catggtgtcg tagatgaccg ggaactggaa gacctgtgcg atattccgct cttccggcga
     1321 ggcgtgcgaa acatcgcggc cgttgaacag gatcttgcct tcggtcggcg tcagcaggcc
     1381 ggagatcaga ttgagcaggg ttgtcttgcc gcaaccagag gggcccagca gcgcgtaggc
     1441 gccgccgtcc tgccaggtca gtgacaggcg cttcagtgca tagtcctcgt cggctccggg
     1501 atttgcgagg tagctgtgcc ggaggttctc gatgcgtatc tctgccatta tgacaccagc
     1561 tcacccgatg gcaggaagaa gagtccgcgg tcgacctcga catgaagcct cgcctgggag
     1621 ccgacctcga aaggatggat gccgtgagac tgggacaccc aggtcttgcc agcaacactc
     1681 aggtgaatga cgctctcgga gccggagagt tcggtaacca ggacgcggcc ttcgatttcg
     1741 cctgtcttgg ccttgaccgg caggggcgtg acatagtgcg ggcggacgcc aaagatgtat
     1801 cggccgtcag gcaggttttc ggcctcacgc ccggcgggcc aagactggtc accgaagacc
     1861 gtcagcttgc tgccgcgctt ttcgacgtcg gcgatgttga tcggcggatc cgaaaagacc
     1921 tgggccgtgg tgaggtcgcg gggcgcacga taggtggctg atgttggccc gaattgggtc
     1981 acccggccct catgaagggt ggccgtgttg ccgccgaaca gcagcgcctc ggagggctcg
     2041 gtggtggcat agacgacgat ggcgttgcgg ccggcgaaga gcttgggcag ttcgtcgcgc
     2101 agttcctcgc gcagcttgta gtcgaggttg gccagcggct catccatcag gatcaggtcg
     2161 gagtctttga cgatggcccg cgcgatggcg gtgcgctgct gctgtcctcc ggagagttcc
     2221 gagggcctcc gcttgagcat ggacttcaag cggaggagtt cggcggcggc gccgaccctg
     2281 cggtcgattt cggcggcgct catgccggcc actttcagtg gcgaggcgat gttgtcgtag
     2341 accgtgaagt tcggatagtt gatgaactgc tgatagacca tcgagacatt gcggcgctgg
     2401 accggaaacc ttgtcacatc ctgaccgcgg aacctgatgg tgcccgaact tggcgactgg
     2461 atgccagcca tcagttgcat gagcgtggtc ttgccggaaa gggtcgtgcc cagcagaacg
     2521 ttgaatgaac cttccggcaa gacaagcgat gttggatgaa tgtaggtctc tgcgcccaca
     2581 cgcttggtga tgtcgatcag ttcgagggac atgagcctgt gcttccagcg caagtgagcc
     2641 gtagcggtct ggccggaacg gcagggaagg ggagcgcggg aaaaagtccc cgcgctcctg
     2701 actggaaagc tattcctgcc agcgcttgac gatttcctcg taggcgatgg tttgacccgg
     2761 ctccttctcg ttctcgagct tggccttggg gccatccggc ttgccgagcc actcggacgg
     2821 atccttgggt tccgccagac gggggccgca accaccatag gtgttgttgg ccttgtcggc
     2881 ggcttccatg cgctccatca cctgttccat ttcgctggcg agacggtcca tggcttcctg
     2941 cggcgtgaag gcgccggagt tcacgtcacc gatctgctgc caccagagtt gggcgagctt
     3001 cggataatcc ggcacgttga tgccggtggg cgaccagcgc acgcggtcgg gcgagcgata
     3061 gaactcaacg agaccgccaa gcttgggcgc acgttccgtg aagctgtcat gccgcacggt
     3121 ggagtcgcgt atgaaggtga gcccaacctg actcttcttc acgtcgaccg tcttcgatac
     3181 gacgaactgg gcatagagcc atgcagcctt ggcgcggtct tccggcgtcg acttcaggat
     3241 cgtccacgac cccacatctt gataaccgac cttctggccg tccttccagt agggaccgtg
     3301 cggcgagggg gccatgcgcc acagcggatt gccctggtcg tcgaccgtgt tgttgccttc
     3361 ggacttcggc gcaaccatcg aggcggtgaa ggccgtgtac cagaagatct gctgggccac
     3421 gttgccctgc gccaatgccg gcagcgactg atagaagtca tagtcggccg cacccggagg
     3481 ggcatacttc cggagccatt catcccactt cgcgatggca tagacggaag cagggccatt
     3541 ggtttcgccg ccacgcgcga ccgacgcgcc cgacgggttg catgaaccct tctccatgcg
     3601 aatgccccat tcgtcgatgg ggatgccgtt gggttcgccc acggtgcccg caccggccat
     3661 ggacagccag gcgtccgtca tgcgccagcc gaggtccggc gcacgcttgc cgtaatccat
     3721 atgtccgtag atcttcacgc cgtcgatttc cttcacgtcc tccgtaaaga actgggcgat
     3781 gtcctcatag gcagaccagt tcacggggac gccaagctca tagccatatt tcgccttgaa
     3841 cttgtccttg aggtcctgcc ggtcaaacca gtccttgcgg aaccagtaaa gattggcgaa
     3901 ttgctgatcg ggcagctgat agagtttccc gtccggaccc gtggtgaact tgattcccat
     3961 gaagtcattg agatcgagac ctggattggt gacgtccttg ccggcgcctg ccatgaagtc
     4021 ggtcaggttg acggcctgtt gcaggcggga gtgcgtgccg atcagatcgg agtcgttgac
     4081 ataggcgtcg tagagattgc ggtttgtctg catctgggtc tgcacggcct gcacgacttc
     4141 gccttcgccc agaatctggt gattgacctt gatcccggtg atttcctcga aggccttggt
     4201 cagcacggtg gattcgtagc tgtgcgtcgg aatgccttca gacagcacgt tgatttccat
     4261 gcccgcaaag ggcttggacg cctcgatgaa ccactccatc tcggcctgtt gttgct
//
LOCUS       DBNGVR010000421         4000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3298921, whole genome shotgun sequence.
ACCESSION   DBNGVR010000421 DBNGVR010000000
VERSION     DBNGVR010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3298921"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..495)
                     /locus_tag="AC16A0_08920"
     CDS             complement(4..495)
                     /locus_tag="AC16A0_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR3105498.1"
                     /translation="MESANPEPEPRAVAKVRGAAMAAGLEVRIAIMPESTHTAEDAAA
                     ACGCDVGQIVKSLVFRGKDSRRPILLLVPGRNRVDTVKVAAVIGEALDRPDADFVRAV
                     TGYAIGGIPPIGHDTPMATYLDTDLLGYPEVFAAAGTPNAIMTVNPAALRKAAAAIET
                     KVA"
     gene            612..1109
                     /locus_tag="AC16A0_08925"
     CDS             612..1109
                     /locus_tag="AC16A0_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105499.1"
                     /translation="MTQALRQARAAEAAHADAVFALRDAKAIRLQLLKDDLMPIIAAL
                     PQSEHLFDLALVPGDPPRLWIDLISYVVMEPEHRSYRLYEDRQSGRGILFESEDRALM
                     VERIRRHMAHRLVARQHQIASVTSADRPAGFSVGSVILAWLAGFALGALALLAAAILL
                     GKLEI"
     gene            1256..3043
                     /locus_tag="AC16A0_08930"
     CDS             1256..3043
                     /locus_tag="AC16A0_08930"
                     /inference="COORDINATES: protein motif:HMM:NF006624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HIR3105500.1"
                     /translation="MTKTLSLAHDHENQREVVRTPRSNRQLPLLLAPFATLAEGLDYA
                     AQGVTGFNFYSARGALQDVLPYALLRRRALSTARKLLSLGFERGDRVAVVAETGPDFI
                     VVFFACQYAGLVPCPMPYTMYIGGKDAYVERVAGMLRAARAGTVITPEDLLGHISEGA
                     DRAGAKRVLTFGDLQSLPEAATKLEPFRADEQAYIQYSSGSTSSPKGVLISQAAIAAN
                     TRGILRHGMRITQDDRAFSWLPLYHDMGLVGFCLAPMMGQCTVDFLATTSFARRPALW
                     LKLMSDNQSTITYSPSFGYDLAARRINGDAPSLNLSRLRVAGIGGDMVRADVLQLFAD
                     TLGVAGFRSAAFLPSYGMAETTLAISFVDADAPFRIDSIDRHALKTEARAVPAKGEAA
                     RSFVVCGKPMPDSVVEIRNDSGQPLGERRVGRIFVKSPSLMAGYYHNEDATSAAVGSD
                     GFLDTGDMGYWLDGEIVITGRAKDLILHNGRNIWPQDIEWAAEHIEPLRSGDVAAFAV
                     EGRDGDDDVVVLVQCRLQDEVSIESLRHAVSAAIHRSAGVECKIVMVPPKSLPFTSSG
                     KLSRSAAKQKYLSGEIAEIMQPVFLQAAQ"
     gene            3075..>4000
                     /locus_tag="AC16A0_08935"
     CDS             3075..>4000
                     /locus_tag="AC16A0_08935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3105501.1"
                     /translation="MDKTIAVTGATGFAGGHAVSELLSRGHRLRALVRDPQRAHLPAA
                     VEVVQGDLFDAEALDRLLRGADAVLHLAGAISALTEHDYFRVNAHGTLALAEASLRHG
                     VKRFVHVSSLSARAPQLSPYGASKRAGEDAVFKLMARLNAMILRPPAVYGPGDRATLP
                     LLRELTRPVAVIPGRPGNRFSLVQAADLARFAADALVNPADGLHEISDGTPNGYGWDE
                     LLDIAGRERGGPIRAIFLPRAMPQTIAVAAEALARATGKPGMINRGKIAELYHPDWVA
                     RDGGLVLPAPIRFATGLPETLAWYRQAGWLPRA"
BASE COUNT          640 a         1281 c         1286 g          793 t
ORIGIN      
        1 gccctaggca accttcgtct cgatggccgc tgccgccttt ctcagcgcag cggggttgac
       61 cgtcatgatg gcgtttggcg ttccagcggc cgcaaagacc tccgggtagc caaggagatc
      121 ggtgtcgagg tatgttgcca tcggcgtgtc gtgaccgatg ggcggaatgc cgccgattgc
      181 atagccggtc accgcgcgca cgaaatctgc atcgggccgg tcgagggctt ctccgatgac
      241 ggcagccacc ttgaccgtgt cgacacggtt gcggcccggg acgaggagca ggatcggacg
      301 tcgggaatcc ttgccgcgga agaccagtga cttcacgatc tggcccacgt cgcatccgca
      361 ggcggccgcg gcgtcctcgg cggtgtgggt gctctcgggc atgattgcga tgcggacctc
      421 aagtccagct gccatggcag cgccacggac cttggctacg gctcgaggtt ccggttcggg
      481 attggcactt tccatgcagg atccggctgt atctattgca acgggcgtta actgtgccag
      541 tctggcacgg tgcgcaggcc tggagttgaa gtttggtcaa tgccggcaca tcattcaagg
      601 acgatgctgg attgacgcag gcactgcgcc aggcccgcgc ggcggaggcc gcccatgccg
      661 atgcggtttt cgcgctccgc gacgcgaagg caattcggct ccagctgctg aaggacgatc
      721 tcatgccgat catcgcggca cttccgcagt ccgagcatct gtttgatctt gccttggtac
      781 ctggcgatcc gccgcgcctc tggatcgatc tcatctccta tgtggtcatg gagcctgaac
      841 accgcagcta ccggctctac gaggatcgcc aatcgggccg tggaatcctc ttcgaaagcg
      901 aagaccgtgc gctcatggtt gagcggatcc gccggcacat ggcccatcgc ctggtggcgc
      961 ggcagcatca gattgcttcc gtgacgtcag cagacaggcc cgcgggcttc tcggtcggat
     1021 cggtgatcct ggcctggctg gccggattcg cgcttggtgc gcttgccctc ttggcggcag
     1081 ctatcctttt gggaaagctt gagatttagc ctgccacatt ggcgttacat tctgaaatac
     1141 cttttgattt cagttccatc ccggcgcctg ttagccctta acagaggggc aagacaatct
     1201 aaggaatcat gcggactttc gggtctgcgt ttcaagttca ctacggggtg gcagtttgac
     1261 gaagactttg agcctggcgc acgatcacga gaatcaacgt gaggtggttc gtacgccgcg
     1321 cagcaatcgg cagcttccgc tgctcctcgc accattcgcc acacttgccg aaggcctgga
     1381 ctatgccgca cagggcgtga cgggcttcaa cttctattcc gcccgcggtg cgttgcagga
     1441 tgttctgccc tatgcgctgc tgcgccgccg ggcgctttcg accgcgcgca agctcctgtc
     1501 gcttggcttt gaacgtggcg accgggtggc cgtcgttgcg gagactggcc cggatttcat
     1561 tgtcgtcttc tttgcctgcc agtatgccgg cctcgttccc tgcccgatgc cctatacgat
     1621 gtatatcggc ggaaaggacg cctatgtcga gcgtgtggcg ggcatgctgc gtgcagcgcg
     1681 cgccggaacg gtcattaccc cggaagacct tctcggccac atctcggaag gtgccgaccg
     1741 cgctggcgca aagcgcgtcc tgaccttcgg cgatctgcaa tccctgccgg aagcagccac
     1801 caagcttgag ccattccggg cggacgagca ggcctatatc cagtattcct cgggttcgac
     1861 ctccagcccc aagggcgtgc tgatttcgca agcggccatt gccgccaata cccggggcat
     1921 tctgcgccac ggcatgcgca tcacccagga tgacagggct ttctcgtggc ttccgctcta
     1981 tcacgacatg ggccttgtcg gcttctgcct ggcgccgatg atgggccagt gcaccgtcga
     2041 ctttctcgcc acgacgtcct tcgcccggcg cccggcgctg tggctgaagc tgatgtcgga
     2101 caatcagtcg accatcacct attcgccgag cttcggatac gatcttgcgg cgcggcgcat
     2161 caatggcgat gcgccctcgc tcaacctgtc gcgcctgcgc gtggcaggca tcggcggcga
     2221 catggtgcgc gccgacgtgc tgcaactctt cgccgatacg cttggcgtgg ccggcttcag
     2281 gtctgccgcc ttcctgccca gctacggtat ggccgagaca acgctggcca tttcctttgt
     2341 cgatgccgat gcgcctttcc gtatcgactc gatcgaccgt catgcgctca agaccgaggc
     2401 gcgtgccgta ccggccaagg gcgaggctgc gcgcagcttc gtggtctgcg gcaaacccat
     2461 gccggacagc gtcgtcgaga tccgcaacga ctcgggccag ccgcttggcg aacgccgggt
     2521 cgggcgcatc ttcgtgaagt cgccgagcct gatggcgggt tactatcaca atgaggatgc
     2581 cacctccgcg gcggtgggga gcgatggatt cctcgacacc ggcgacatgg gttactggct
     2641 ggacggtgag atcgttatca ccggccgcgc caaggatctc atccttcaca atggccgcaa
     2701 tatctggccg caggacattg aatgggcagc cgagcatatc gagccgctgc gttcgggtga
     2761 cgttgctgcc ttcgccgtcg aaggccggga tggcgacgac gacgtggtgg tgctggtaca
     2821 atgccggctt caggatgaag tttcgatcga gtcgctgcgc cacgccgtgt cagcggccat
     2881 ccaccggagc gccggcgtcg agtgcaagat cgtgatggtg ccgcccaaga gcctgccctt
     2941 cacctcgtca ggcaagctgt cgcggtccgc agcgaagcag aagtatcttt ccggcgaaat
     3001 cgccgagatc atgcagcctg tgttcttgca ggcggcgcaa taggcccgga ttcgtccggg
     3061 acttcacaag gccgatggac aagacaattg cagttaccgg cgccaccggc tttgccggtg
     3121 gacatgccgt cagcgaactc ctgagccgtg gccaccggtt gcgcgcattg gtgcgcgatc
     3181 cgcagcgcgc gcacctgccg gcggcagtcg aggtggtgca gggcgatctc ttcgacgccg
     3241 aagcgctcga tcgcctgctg cggggcgccg atgcggtgct gcatcttgcc ggcgccatct
     3301 cggcactcac cgaacacgac tacttccggg tcaacgcgca tggcacgctg gcgctggccg
     3361 aggcgtcgtt acgccatggc gtgaagcgct tcgtgcatgt ctcgtcgctc tccgcccgcg
     3421 cgccgcagct gtcgccctat ggcgccagca aacgggcagg ggaggacgcg gtgttcaagc
     3481 tcatggcccg tctcaacgcg atgatcctcc gcccgcctgc cgtctatggc ccgggcgatc
     3541 gtgccacgct gccgctgctg cgtgaactga cacggcctgt tgccgtcatt ccgggccggc
     3601 ccggcaatcg cttttcgctg gtccaagcgg cggatctggc gcgttttgcc gccgatgcgc
     3661 tggtcaatcc ggccgacggt ctgcacgaga tcagcgacgg cacgcccaac ggctatggct
     3721 gggatgaact tctggacatt gccgggcgcg agcggggcgg gccgatccgc gccatatttc
     3781 tgccccgggc tatgccgcaa accatcgcgg tggcggccga ggcgctcgcc cgcgctaccg
     3841 gcaagccggg catgatcaat cgcggcaaga tcgcagagct ctatcacccc gactgggtgg
     3901 cgcgcgacgg cgggctggtt ctgccagcgc cgatccgatt tgcgacaggc cttccggaga
     3961 cgctggcgtg gtatcgccag gccggatggt tgcctcgggc
//
LOCUS       DBNGVR010000422         6015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4870347, whole genome shotgun sequence.
ACCESSION   DBNGVR010000422 DBNGVR010000000
VERSION     DBNGVR010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4870347"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16A0_08940"
     CDS             complement(<1..670)
                     /locus_tag="AC16A0_08940"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3105502.1"
                     /translation="MRDIDTSFAGAAVQAEGGQGTAGDYFALLKPRVMSLVIFTAFVG
                     IVAAPGTIHPWIAAVALLAIAVGAGAAGALNMWYDADIDAAMRRTASRPVPRGAILPG
                     EALGFGMTLAVGSVLALGLLVNVAAGALLAFTIFFYIVIYSMWLKRLTPQNIVIGGAA
                     GAFPPMVGWVSVTGSVDLGSIALFLIIFMWTPPHFWALALWKKINEDYSNAGVPMLPV
                     VAGPD"
     gene            complement(675..2324)
                     /gene="ctaD"
                     /locus_tag="AC16A0_08945"
     CDS             complement(675..2324)
                     /gene="ctaD"
                     /locus_tag="AC16A0_08945"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR3105503.1"
                     /translation="MAHASAAHDDHALPTGWKRYVYSTNHKDIGTMYLWFAIFAGVVG
                     GILSIFMRMELSEPGIQYFSGLASMVYGTPADAALDAGKHMYNVFVTAHGLLMIFFMV
                     MPAMIGGYGNWFVPLMIGAPDMAFPRMNNISFWLLPPALALLVLSMFVEGPAGAMGVG
                     GGWTIYPPLSTSGQPGPAMDLAILSLHIAGASSILGSINFITTIFNMRAPGMTLHKMP
                     LFVWSVLVTVFLLLLSLPVLAGGITMLLTDRNFGTSFFVPSAGGDPVLFQHLFWFFGH
                     PEVYILILPGFGIISQIVSTFSKKPVFGYLGMAYAMVAIGVVGFIVWAHHMYTVGLDA
                     DTQAYFVAATMIIAVPTGVKIFSWIATMWGGSIEFRAPMIWAMGFIFLFTVGGVTGVV
                     LANAGADRVLHDTYYVVAHFHYVLSLGAVFAIFAGWYFWFPKMTGYMYSESLAKLHFW
                     VTFIGVNMLFFPQHFLGLAGMPRRYIDYPAVYAGWNQISSYGSYISALGVVIFLYGVF
                     SAYARKVPAGDNPWGPGATTLEWTLSSPPPFHQFNTLPVIK"
     gene            complement(2349..3230)
                     /gene="coxB"
                     /locus_tag="AC16A0_08950"
     CDS             complement(2349..3230)
                     /gene="coxB"
                     /locus_tag="AC16A0_08950"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3105504.1"
                     /translation="MKFAKTLTVLCGMAASLSPAMAEGTHAKNWQMGLIDSASPVSDY
                     IHWFHNGLLLPIITVISLFVLALLIYVMVKFNSRANPVPSKTTHNTFIEVVWTVVPIL
                     ILVVIAIPSFRLLYLQRDIPKPDMTIKIIGNPSWNWTYEYPDLGVDDTGAAKVSFTAY
                     LKSEEDAAKDGSPYLLATDVPVVVPVNKIVKTIVTSDPEGIIHAWTIPAFGMKIDAIP
                     GRLNEDWFQATREGIFYGQCSELCGKDHAYMPIEVWVVSEANYKAWSDLRLSGADDAA
                     INELLKTIKPAQQVAQN"
     gene            3570..4187
                     /locus_tag="AC16A0_08955"
     CDS             3570..4187
                     /locus_tag="AC16A0_08955"
                     /inference="COORDINATES: protein motif:HMM:NF018482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HIR3105505.1"
                     /translation="MAAVFGLCALAVAGPDVLAQSQPAQQQQQQQQFGPRANKTGLPG
                     QGQGQGQAPKVEEIAKHGAWKVQCESAPPQAEGQQAPPRSCGMVQSAVNEKNPKVNLS
                     LVLVKSKQGDKTVTMMRVLAPIGVYLPTGVALEVDGNAVGRVPFTRCMPQICMAFAEA
                     SPETLEKMRKGGSANFIIYEAPGIGMPMKLSLEGFSKALETLDSL"
     gene            4264..5691
                     /gene="tldD"
                     /locus_tag="AC16A0_08960"
     CDS             4264..5691
                     /gene="tldD"
                     /locus_tag="AC16A0_08960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR3105506.1"
                     /translation="MSTSPVPSLIETSGLNRSAVQALVSDTLRDAEDGELFLERRQSE
                     NLVFDDGKLKGASFDESQGFGLRAVRGETAAYAHGTELSLAALRRASEVCRTVAHGDG
                     VTMSLSPPRTNRHLYAETNPASAMSFAAKVSLLEKADAFARALDPRVKQVSVSMSADW
                     QSVEIIRADGSVYKDIRPLVRFNASVVSSNGKEQGQGSHGLGGRGEPLSYFEDHVWQE
                     AVREALRQSLISLEARPAPAGEMDVVLGSGWPGILLHEAIGHGLEGDFHRKKTSVFTR
                     LMGERIAAKGITVVDNGTLPDRRGSITIDDEGTPSGCTTLIEDGILVGLMQDRLNARL
                     MGVSPTGNGRRQSYAHAPMPRMTNTYMLSGDKHPEEILGSVKRGLYAKSFGGGQVDIT
                     SGKFVFSCTEAYLIEDGKIGAPVKGATLIGAGADALKRISMVGNDSALDPGVGTCGKQ
                     GQGVPVGVGQPSLRIDGLTVGGTAA"
     gene            5695..>6015
                     /locus_tag="AC16A0_08965"
     CDS             5695..>6015
                     /locus_tag="AC16A0_08965"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3105507.1"
                     /translation="MKPRTVIFDMDDVLCRYDLGKRLRALSRITGKTPRDIRAAIWDS
                     GFEDDADSGGYIDPEEYLDQFGRRIGYAISRDEWVGARSHAMTPSSDVLALARSIAAQ
                     ARVAL"
BASE COUNT         1174 a         1894 c         1975 g          972 t
ORIGIN      
        1 catcggggcc tgccacgacg ggaagcatcg gcacgccggc attgctgtag tcctcgttga
       61 tcttcttcca cagggccagg gcccagaaat gcggcggcgt ccacatgaag atgatgagga
      121 acagggcgat cgagccgagg tccacgctgc cggtcaccga gacccagcca accatcgggg
      181 ggaaggcgcc ggcggcaccg ccgatgacga tgttctgcgg cgtcagccgc ttcagccaca
      241 tcgaatagat gacgatgtag aagaagatgg tgaaggccag cagcgcaccg gcggcgacat
      301 tgacgagaag ccccagcgcg aggaccgagc cgacggccag cgtcatgccg aagccgagtg
      361 cctcgccggg caggatggcg ccgcgcggca ccggccgcga ggcggtccgc cgcatcgccg
      421 cgtcgatgtc ggcgtcgtac cacatgttca gggcaccggc cgcgccggcg ccgaccgcga
      481 ttgccagcag cgccacagcc gcgatccagg gatggatggt tcccggtgcg gcgacgatcc
      541 ccacgaaggc ggtgaagatg acgagcgaca tcacccgcgg tttcagcagc gcgaagtaat
      601 cgcccgccgt gccctgcccg ccttcggcct ggacggctgc gcctgcgaaa ctggtgtcga
      661 tgtcgcgcat cctcttactt gatcaccggc agcgtgttga actggtggaa cggcggcggc
      721 gaggacagcg tccactccag cgtcgtggcg cccgggcccc agggattgtc accggccggc
      781 accttgcggg cataggccga gaagacgccg tagaggaaga tgaccacgcc cagggccgag
      841 atataggagc cgtaggacga gatctggttc cagccggcat agaccgccgg atagtcgatg
      901 tagcggcgcg gcatgccggc gagtccgagg aagtgctgcg ggaagaacag catgttcacg
      961 ccgatgaagg tcacccagaa atgcagcttg gccaggctct cgctgtacat gtagccggtc
     1021 atcttgggga accagaagta ccagccggcg aagatcgcga agaccgcgcc cagcgagagc
     1081 acgtagtgga aatgcgccac gacgtagtag gtgtcatgca gcacgcggtc ggcacccgca
     1141 ttggcaagaa ccacgccggt gacgccgccc accgtgaaga ggaagatgaa gcccatggcc
     1201 cagatcatcg gcgcccggaa ctcgatcgat ccgccccaca tggtggcgat ccaggagaag
     1261 atcttcacgc ccgtgggaac cgcgatgatc atggtggcgg ccacgaaata ggcctgcgtg
     1321 tcggcatcga ggcccaccgt gtacatgtga tgggcccaga cgatgaagcc caccacgccg
     1381 atcgccacca tggcataggc catgccgaga tagccgaaga cgggcttctt cgagaaggtc
     1441 gagacgatct ggctgatgat gccgaagccc ggcaggatca ggatgtagac ttccggatgt
     1501 ccgaagaacc agaagagatg ctggaacagc acgggatcgc cgccggccga gggcacgaag
     1561 aacgaggtgc cgaaattgcg gtcggtcagc agcatggtga tgccgcccgc cagcaccggc
     1621 agcgacagca gcagcaggaa cacggtgacc agcaccgacc agacgaaaag cggcatcttg
     1681 tgcagcgtca tgccgggcgc gcgcatgttg aagatggtgg tgatgaagtt gatcgatccg
     1741 aggatcgacg aggcacccgc gatgtgcagc gagaggatcg cgagatccat cgccgggccg
     1801 ggctggcccg atgtcgacag cggcgggtag atggtccagc cgccgccgac gcccatggca
     1861 ccggcaggcc cttcgacgaa catcgagagc accagcagcg ccagcgcggg cggcagcagc
     1921 cagaaggaga tgttgttcat gcgcgggaag gccatgtcgg gggcgccgat catcagcggc
     1981 acgaaccagt tgccatagcc gccgatcatg gccggcatga ccatgaagaa gatcatgagc
     2041 aggccgtggg cggtcacgaa gacattgtac atgtgcttgc cggcatcgag ggcggcatcc
     2101 gccggcgtgc cgtagaccat cgaggcgagg cccgagaaat actggatgcc gggctcggac
     2161 agctccatgc gcatgaagat cgagagaatc ccgccgacca cgccggcgaa gatcgcgaac
     2221 cacagataca tcgtgccgat gtccttgtgg ttggtggagt agacgtagcg cttccatccg
     2281 gtcggaagcg cgtgatcgtc atgagcggct gatgcgtgtg ccatgtgtct tcgccttgtc
     2341 ccctgaactc agttctgcgc aacctgctgc gcgggcttga tggtcttgag aagctcgttg
     2401 atcgcggcat cgtccgcgcc ggacagccgc aggtcgctcc aggccttgta gttcgcttcc
     2461 gagacgaccc acacctcgat cggcatgtag gcatggtcct tgccgcacag ctccgagcac
     2521 tggccgtaga agatgccttc gcgggtggcc tggaaccagt cctcgttcag gcggccgggg
     2581 atggcgtcga tcttcatgcc gaaggccggg atcgtccagg cgtggatgat gccctccgga
     2641 tcggaggtga cgatggtctt gacgatcttg ttcaccggaa ccaccaccgg cacgtcggtc
     2701 gccagcagat agggcgaacc gtccttggcc gcgtcctctt ccgacttgag gtaggccgtg
     2761 aagctcacct tcgcggcgcc ggtgtcgtcc acgccaaggt cgggatactc gtaggtccag
     2821 ttccagctcg gattgccgat gatcttgatc gtcatgtcgg gcttgggaat gtcgcgctgc
     2881 aggtagagca ggcggaagga cggaatggcg atcaccacga ggatcaggat gggcaccacc
     2941 gtccagacca cttcgatgaa ggtattgtgc gttgtcttgg agggaaccgg gttcgcgcgg
     3001 gagttgaact tcaccatcac gtagatcagc agggcgagaa cgaagagcga aatcaccgtg
     3061 atgatgggca gcagcagccc attgtggaac cagtggatat agtccgacac gggcgatgcc
     3121 gaatcgatca gacccatctg ccagttcttc gcatgcgtac cttcggccat ggctggcgaa
     3181 aggcttgcgg ccatgccgca gagcaccgtc aacgtcttcg cgaacttcat cgtcgcccct
     3241 ttccctctca tccccccgtc ctgccccagg gacacgacgg atcaagctgt cagcctgttg
     3301 acggctcagc tccggcgcga ttgtgaacca tattcgacgc ctgcccgcaa cggaggcagg
     3361 cgcggcaaaa cgtcattgcg cgcgcagcag cggggctgac cggaattccg ccccaatttg
     3421 ctccgaaacc gccgtcatcc gagacaatgg tcgctgcggt atcgctgata gaagattgtt
     3481 gtcatgtccg gaccccggcg ctatgagccg aaagcttggt caagaggtgg ttttccgtgc
     3541 gaatcaattc catgttgcga aatgcggcca tggccgctgt gttcggtctt tgtgccctgg
     3601 cggtcgccgg tcctgacgtt ctggcgcagt cccagcccgc gcagcagcag cagcaacagc
     3661 agcagttcgg gccgcgcgcc aacaagaccg gccttcccgg acagggccag ggccagggcc
     3721 aggccccgaa ggtcgaggag attgccaagc acggtgcctg gaaggtgcag tgcgaaagcg
     3781 cgccgccgca ggccgaaggc cagcaggccc cgccgcgcag ctgcggcatg gtgcagtcgg
     3841 ccgtcaacga gaagaacccg aaggtcaatc tcagcctcgt gctggtgaag tccaagcagg
     3901 gcgacaagac cgtcacgatg atgcgcgtcc tcgcccccat cggcgtctat ctgccgacgg
     3961 gcgtcgccct cgaggttgac ggaaacgccg tgggccgcgt gcccttcacc cgctgcatgc
     4021 cgcagatctg catggccttt gccgaggcct cgccggaaac gctggagaag atgcgcaagg
     4081 gcggcagcgc caatttcatc atctacgagg cacccggcat cggcatgccg atgaaactgt
     4141 ccctcgaagg cttctccaag gctctcgaaa cgctcgacag cctctgatcg gtccttcact
     4201 gacactggcg gaaacgggcc ggttcaccta tctagaggcg gtccccagga aaggtccgcc
     4261 cccgtgtcga cgtcccccgt tccatctctc atcgaaacct ccggcctgaa ccggagcgcc
     4321 gtccaggcgc tggtctccga cacgctgcgc gacgcggaag acggcgagct gttcctcgaa
     4381 cgccgccagt cggaaaacct cgtcttcgac gatggcaagc tcaagggcgc aagcttcgac
     4441 gaatcgcagg gtttcggcct gcgggcagtc cgtggcgaaa cggcggccta cgcccatggc
     4501 accgagctca gcctcgccgc cctccggcgg gcgtcggaag tctgccggac cgtcgcgcat
     4561 ggcgacggcg tcaccatgag cctgtcgccg ccgcgcacca accggcatct ctatgccgag
     4621 accaatcccg cgtcggccat gagctttgcg gcaaaggtca gcctgctgga aaaggcagac
     4681 gccttcgcgc gcgcgctcga tccgcgggtc aagcaggttt ccgtctcgat gtcggccgac
     4741 tggcagtcgg tcgaaatcat ccgtgccgac ggcagcgtct acaaggacat tcgccccctg
     4801 gtccgcttca atgcttcggt ggtctcctcc aacggcaagg aacagggaca gggcagccac
     4861 ggcctgggcg gacgtggcga gccgctctcg tatttcgagg atcatgtctg gcaggaggcc
     4921 gtgcgcgagg cgctgcggca aagcctgatc tcgctcgagg cccggccggc gccggccgga
     4981 gagatggacg tggtgctggg atcgggctgg cccggcattc tcctgcatga ggccatcggg
     5041 cacgggctgg agggagactt ccaccgcaag aagacatcgg tcttcaccag gctcatgggc
     5101 gaacgcattg ccgccaaggg gatcacggtc gtcgacaacg gaacgctgcc ggatcggcgc
     5161 ggctcgatca ccatcgacga tgaaggcacg ccatcgggct gcaccacgct catcgaggat
     5221 ggcattctgg ttggcctcat gcaggaccgc ctcaatgcgc gcctcatggg tgtgtcgccc
     5281 accggcaacg gacggcgcca gtcctatgcc catgcgccca tgccgcgcat gaccaacacc
     5341 tacatgctgt cgggcgacaa gcatccggag gagattctgg gctcggtcaa gcggggcctc
     5401 tacgccaagt ccttcggtgg cggccaggtg gacatcacca gcggcaagtt cgtgttttcc
     5461 tgcaccgagg cctatctgat cgaagacgga aagatcggcg ctcccgtcaa gggtgccacg
     5521 ctgatcggcg ccggcgcgga tgccctcaag cgcatctcga tggtcggcaa tgactccgca
     5581 ctcgatccgg gggtcggaac ctgcggcaag cagggacagg gcgtgccggt gggcgtgggc
     5641 cagcccagct tgcgcattga cggattgacc gttggcggca ctgcggcgtg aaccatgaaa
     5701 ccgcgcacgg tcatcttcga catggatgat gtcctgtgcc gctatgatct cggcaagcgg
     5761 ctgcgcgccc tgtcgcggat caccggcaag actccgcgcg atatccgggc cgcaatctgg
     5821 gattcgggct ttgaagacga cgccgactcg ggcggctata tcgaccccga agaatatctc
     5881 gaccaattcg gccgccgcat cggctatgcc atcagccgcg atgaatgggt cggagcccgc
     5941 agccatgcca tgacgccgtc gtctgacgtg ctcgctctcg caaggtcgat tgccgcacaa
     6001 gcgcgggtgg cgctc
//
LOCUS       DBNGVR010000423         2465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2709540, whole genome shotgun sequence.
ACCESSION   DBNGVR010000423 DBNGVR010000000
VERSION     DBNGVR010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2709540"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="AC16A0_08970"
     CDS             <1..1012
                     /locus_tag="AC16A0_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR3105508.1"
                     /translation="RLPSAIDNRPLRFEEWDAMRPQTVCVSATPGSWEMNQTGGVFAE
                     QVIRPTGLIDPPVEIRPVKTQVDDLVDECKKVALAGYRTLVTTLTKRMAEDLTEYMHE
                     QGIRVRYMHSDIDTLERIEIIRDLRLGAFDALIGINLLREGLDIPECALVAILDADKE
                     GFLRSETSLVQTIGRAARNVDGKVILYADRITGSMERAIAETDRRREKQVAYNEANGI
                     TPQSIKRNINDILKSVYEQDHVTADIEAPLIGHNLEKVLADMEKRMREAAANLEFEEA
                     ARLRDEVKRLRAVELAVKDDPMATQSAIEDASASWPKPKGRAAGSKGGKPGTRTYRGK
                     GR"
     gene            1319..1672
                     /locus_tag="AC16A0_08975"
     CDS             1319..1672
                     /locus_tag="AC16A0_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105509.1"
                     /translation="MRKTMFAAGAALMIMAMPAAAELAKGDAITAAISGNTVQGNMDS
                     TGPYAEHYGADGVVHGKDYKAKWSVEGDTMCWVYDGTPKDCWSVEIAGDQVRWLKDGK
                     SLGTGTILPGNPNNF"
     gene            1800..>2465
                     /locus_tag="AC16A0_08980"
     CDS             1800..>2465
                     /locus_tag="AC16A0_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3105510.1"
                     /translation="MNGAAYIKGRYMPFAEATIPVTDWGFTRSDVVYDVVHVWQHRFF
                     RLEDHLDRFAASMAKRQLALAEDRAQLADVLHRCVALSGLADAFVAMVATRGKPRIYG
                     SRRPADCENYLIAYAIPFVQVLTPEVQARGAHLWIASTPRVPDASVDPAVKNYQWSDM
                     TAGLLEGHDAGFDTALNCDAEGFVTEGPGFNVFVVKDGRLTTPDRGSLGGITRKTVLE
                     ICAD"
BASE COUNT          477 a          820 c          755 g          413 t
ORIGIN      
        1 ccgcctcccc tcggccatcg acaaccgccc cctccgcttc gaggagtggg acgcgatgcg
       61 cccgcaaacc gtgtgcgtct cggccacccc tggctcctgg gaaatgaacc agaccggcgg
      121 cgtcttcgcc gagcaggtca tcaggccaac cggcctcatc gatcccccgg tggaaatccg
      181 cccggtgaaa acccaggtcg acgatctggt ggacgagtgc aagaaggtgg cgctggccgg
      241 ataccgcacc ctcgtcacca ccctcaccaa gcgcatggcg gaggacctca ccgaatacat
      301 gcacgagcag ggcatccgcg tccgctacat gcattcggat atcgacacgc tggagcggat
      361 cgagatcatc cgcgacctgc gcctcggcgc cttcgatgcc ctgatcggca tcaacctgct
      421 gcgcgagggc ctcgacattc ccgaatgcgc cctcgtcgcg atcttggatg ccgacaagga
      481 aggcttcctg cgctccgaaa cctcattggt ccagacgatc ggccgcgccg cccgcaatgt
      541 cgacggcaag gtgatcctct atgccgaccg gatcaccggc tcgatggaac gcgccatcgc
      601 cgagaccgac cgccgccgcg aaaagcaggt cgcctacaac gaagccaacg gcatcacgcc
      661 gcagtcgatc aagcgcaaca tcaacgacat cctcaagagc gtctacgagc aggaccacgt
      721 cacggcggat atcgaagccc cgctcatcgg ccacaacctc gaaaaggtcc tggccgacat
      781 ggaaaagcgc atgcgcgagg ccgccgccaa cctggaattc gaggaggccg cacgcctgcg
      841 cgacgaggtc aagcgcctgc gcgcggtgga actggccgtg aaggacgatc ccatggccac
      901 gcaaagcgcc atcgaggacg caagcgcgag ctggccgaag cccaagggcc gcgccgccgg
      961 atcgaagggc ggcaagccgg ggacgcggac gtatcggggg aaggggcggt aaagaccgcg
     1021 gtcccgcagt ccggtccgat ctaaatcacc ttcgtcctga tcgcctcgag tttctttccc
     1081 acatcccgct tctgcattcg cagatcatcg tccaattgca ggtgcaggaa aaatcctttg
     1141 catacaacgc aagaagttac acgctatcct cttccgcgct tcgggcactt tcccccgtca
     1201 cagccggaag aaggcaaacc gccgcagctg cggaccttgg tcccgcaatc ggccattgtg
     1261 gtatccgagg aagcccgata cagtttatgc tagcacaatc attcgggagt ttattgatat
     1321 gcggaagacg atgtttgcgg cgggtgcagc gctgatgatc atggcgatgc ccgcggcggc
     1381 cgaactggcc aagggcgatg cgatcacggc tgccatctcc ggcaacacgg tgcagggcaa
     1441 catggactcc accggtccct atgccgagca ttacggcgca gacggcgtgg ttcatggcaa
     1501 ggactacaag gccaagtggt cggtcgaagg cgataccatg tgttgggtct acgacggcac
     1561 gccgaaggat tgctggagcg tagagatcgc aggcgatcag gtaagatggc tgaaggacgg
     1621 caagtctctg ggcacaggaa cgattctgcc cggcaatccc aacaacttct gaggctgcga
     1681 gccggacctt cgggggcgtt gatcgccgcg cgcgtgtgac ggctatcgaa ggcgggcgct
     1741 gcccttcaca ccgtgcctca ttccgtgcat cctgcatcgc catgatagac aacgccttct
     1801 tgaacggcgc cgcctacatc aaaggccgct acatgccttt cgcggaggcc accatccccg
     1861 tcaccgactg gggcttcacg cgctccgacg tggtctatga cgtggtgcat gtctggcagc
     1921 accgcttctt ccgccttgag gatcatctcg accgcttcgc cgcttcgatg gcgaagcggc
     1981 agcttgccct tgccgaggac cgcgcgcagc tggccgatgt gctgcatcgc tgcgtggcgc
     2041 tgtcggggct ggcggatgcc ttcgtcgcca tggtggccac gcggggcaag ccgcgcatct
     2101 atggctcgcg ccgcccggcc gactgcgaga actatctcat cgcctatgcc attcccttcg
     2161 tgcaggtgct gacgcccgag gttcaggcgc gcggcgccca tctgtggatc gcctccacgc
     2221 cccgtgtgcc tgatgcctcg gtcgatcccg cggtcaagaa ctaccagtgg tccgacatga
     2281 cggcgggcct gcttgagggc catgacgcgg gcttcgacac cgcgctcaac tgcgatgccg
     2341 agggattcgt caccgagggc cccggcttca atgtcttcgt ggtcaaggac ggccggctga
     2401 cgacacccga tcgcggcagc ctgggcggca tcacgcgcaa gaccgtgctg gagatctgtg
     2461 ccgat
//
LOCUS       DBNGVR010000424         5182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3299765, whole genome shotgun sequence.
ACCESSION   DBNGVR010000424 DBNGVR010000000
VERSION     DBNGVR010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3299765"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="AC16A0_08985"
     CDS             complement(<1..440)
                     /locus_tag="AC16A0_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR3105511.1"
                     /translation="MTLDVTFGMALLAGIISFLSPCVLPLVPPYLCFITGASLEQLSE
                     REGSMAVQRRRTVLAALFFVLGFSTVFILMGVSASYVGQLLRAWLPVLAQVAGVFIIL
                     MGLNFLGVFKLGFLSREKRYHAETRPAGLIGAYGVGLAFAFGWTP"
     gene            577..1593
                     /locus_tag="AC16A0_08990"
     CDS             577..1593
                     /locus_tag="AC16A0_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HIR3105512.1"
                     /translation="MRLAAVSLAALLAVNAPSLAGPKVVASVVPVHGIVAAVMGDTGT
                     PELLLEGKLSEHRATFTADQISTLGKADVVFIIGHGLEAKLAQISGSEAVNGKTFVEL
                     SEAPGVKTLPIREGGAWEAHDHDHDHGEGGEAAEAGHDHGETAEAGHDHDHDKKAEAG
                     HDHEGVLAFDPHVWLDPENAKAMAAAVAAELSKADPANAKAYEANAAAFAASLDAVSA
                     DIAKELEPAKGKPFIVFHDAYQYFENRFGLDAAGSISDISAQAPSAERLKEVRDKLAA
                     TKAQCVFREPQYDDKVVAVVIEGSAAKSGVLDGLGADLTPGPEAYPQLLRNLASSFHA
                     CLAS"
     gene            1663..2325
                     /locus_tag="AC16A0_08995"
     CDS             1663..2325
                     /locus_tag="AC16A0_08995"
                     /inference="COORDINATES: protein motif:HMM:NF017985.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HIR3105513.1"
                     /translation="MRRFWRLLAPGVAAIGLAGQAAAHPHVWIEMRSDVIFNDEGKIT
                     GVNLIWSFDDGYTQVALEGLDTNGDGVYSQQELDPLTKENIASLKDYEYFTVMRVNGE
                     KVAQGDVTEFGQIWSNDKLQLHFQVPLKEPVDPTRDEFMVKVYDPEFFIAMDYAKDDP
                     VTAIGAMPDACNLVVKPVPTDAEIDATREMLSTKGPDWKPETNEDFGALFAQPVLIEC
                     KR"
     gene            2313..3416
                     /locus_tag="AC16A0_09000"
     CDS             2313..3416
                     /locus_tag="AC16A0_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HIR3105514.1"
                     /translation="MQALSRFAAAIFLGLALLLAATPAAVHALEEAAPAVTIDKRKLL
                     VQPRNADGSRQQVSIFEDPVLWAREKQQSFYGQMSGALRNMKSGSALAAAWTLMLLSF
                     GYGVFHAAGPGHGKAVISAWLLATENELRRGIAISAMSAVIQALTAILIVSILLLLVA
                     SASAKARDVAGFLESASYGLIGLMGLYLIWTAFRSRRASPAPAHAGRDAMHRVSDLHH
                     FDSFPPKQPAHLPHDHVHGPDCGCGHAHLPEARDLRGDWSFARAFSLAFAVGIRPCTG
                     AILVLIFANALGLYWAGIAATFAMALGTFITVSAIAAIAVYSKKLATRYARQDDRWIS
                     WLNFGLRLGGGLVIAFLGSILFLGSLGSTNAMM"
     gene            3425..3889
                     /locus_tag="AC16A0_09005"
     CDS             3425..3889
                     /locus_tag="AC16A0_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015060.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIR3105515.1"
                     /translation="MRNQWLVLAGFIFVCLAAGFGASFATAPAIIEWYPTLNKPWWNP
                     PNWVFGPAWTILYILMAIAAWLVWRQPKDTKGALILFFSQLALNFAWSFLFFGARSPW
                     LGLIGVAFMLAAVAITTVVFWRITKPAGMLFIPYLAWTAFAAFLNLTIWRMN"
     gene            3943..4212
                     /locus_tag="AC16A0_09010"
     CDS             3943..4212
                     /locus_tag="AC16A0_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3105516.1"
                     /translation="MAKVFFTANLRRHVDCSAMEADGVTVGDVLSTVFAIQDRLGAYV
                     LDDQGALRKHMTILVDGQRIRDIERLTDPVRPNSEVWVMQALSGG"
     gene            4225..>5182
                     /locus_tag="AC16A0_09015"
     CDS             4225..>5182
                     /locus_tag="AC16A0_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3105517.1"
                     /translation="MSETLLVSTRKGLFDIGMGKSGWAVTDSAFLGDNVTLALHDRRD
                     GTDYAALNHGHFGVKLHRRDKGGVWTEIAAPAYPPKPERLNDVDGWGRPVKWTTQMIW
                     ALEPGGADEPGTLWAGTMPGGLFRSRDRGESWEMMRPLWDMPERNKWLGGGADIPGIH
                     SICVDPRSSKIVRVAISCGGVWVTHDGGESWAQSAHGMRFDDGPAGVADDPHTQDPHM
                     MVQCPAAPERFWVQHHCGIWRSADNAMSWQEVKARPSSFGFGVVVHPKDPDTAWFVPG
                     IKDEKRYPVDGKLVVTRTRDGGKSFDILDKGLPGSHAYDIVFR"
BASE COUNT          946 a         1668 c         1634 g          934 t
ORIGIN      
        1 ggtgtccagc cgaaggcgaa ggcaaggccc acgccatagg cgccgatcag gcccgccgga
       61 cgcgtttcgg catggtagcg cttctcgcgg ctgaggaacc ccagcttgaa gacccccagg
      121 aagttgagcc ccatgaggat aatgaagaca cctgcaacct gggccagaac cggcagccag
      181 gcgcgcagca gctggccgac ataggaagca ctcaccccca tcaggatgaa gaccgtggag
      241 aatccgagca cgaagaacag cgccgccagc accgtacggc ggcgctgtac ggccatgctt
      301 ccctcgcgct ccgacagctg ctccagcgag gcgccggtga tgaagcagag atagggcggc
      361 accaatggca gaacgcaggg actgaggaag ctgatgatgc cggccagcag ggccatgccg
      421 aaggttacgt cgagggtcat gatcttcaca tacaggcccg gcgcggcggc gcaacctaac
      481 gattggacat gcggacttca cgaccgcgtc agggattgac gctaccggca cagcgtgtat
      541 gttatactat aacataacat accgttggag tacgccatgc gtcttgccgc cgtcagcctt
      601 gctgccctgc ttgccgtgaa cgccccttcc ctcgccggac cgaaggtcgt cgcctcggtc
      661 gtcccggttc atggcatcgt ggcggcggtc atgggcgata ccggaacgcc ggaactcttg
      721 cttgaaggca agctgtccga acatcgcgcc accttcacgg ccgaccagat ttccaccctg
      781 ggcaaggcgg atgtggtgtt catcatcggc catggcctgg aggccaagct ggcccagatc
      841 agcggcagcg aagccgtcaa tggcaagacc tttgtggaac tctccgaggc accgggcgtg
      901 aagactctgc cgatccgcga gggcggcgcc tgggaggccc atgaccacga tcacgatcat
      961 ggcgaaggcg gcgaagccgc agaagccggg catgatcacg gcgagaccgc cgaggccggc
     1021 cacgatcacg accatgacaa gaaggccgag gccggccatg atcacgaggg cgtcctcgca
     1081 ttcgacccgc atgtctggct cgatcctgaa aacgccaaag ccatggccgc cgccgtggcg
     1141 gccgaactga gcaaggccga tcccgccaac gccaaggcct atgaagccaa tgccgcggcc
     1201 ttcgcagcct cgctcgacgc cgtctccgcc gacatcgcaa aggaactgga gccggcgaag
     1261 ggcaagcctt tcatcgtgtt ccacgatgcc tatcagtact tcgagaatcg cttcggtctc
     1321 gatgccgccg gttccatttc ggacatctcc gcccaggcgc catcggcgga acgcctgaag
     1381 gaggtgcgcg acaagctggc agccaccaag gcccaatgcg tgttccgcga gccacagtat
     1441 gacgacaagg tggtggcggt ggtcatcgaa ggcagcgcgg caaagtccgg cgttctggat
     1501 ggtctgggcg ccgacctgac ccccggacct gaggcttacc cgcagttatt gcgcaacctg
     1561 gcgtcctcct tccacgcttg tcttgcaagt taatcgccgg cttcactttt tcgccgcatt
     1621 ggcggatcat ctcacagcga gtcggagttg tgaggacgga ccgtgcggcg tttctggaga
     1681 ctgctggccc cgggtgtggc ggcgattggc cttgcgggcc aggccgcggc gcacccgcat
     1741 gtctggatcg agatgcgatc tgacgtgatt ttcaacgacg aaggcaagat caccggcgtc
     1801 aacctgatct ggagcttcga cgatggatac acccaggttg cgctcgaggg cctcgatacc
     1861 aatggcgacg gcgtctacag ccagcaggag ctcgatccgc tgaccaagga aaacatcgcc
     1921 tcgctgaagg actacgagta ctttacggta atgcgcgtga acggcgaaaa ggtggcgcag
     1981 ggcgacgtca ccgagttcgg gcagatctgg tcgaacgaca agctgcaact gcacttccag
     2041 gtgccgctca aggagcccgt cgatccgacc agggacgagt tcatggtgaa agtctatgac
     2101 cccgagttct tcatcgcgat ggactatgcg aaggatgacc ccgtgaccgc gatcggtgcc
     2161 atgccggacg cctgcaatct tgtggtgaaa cccgtcccga ccgacgccga gatcgacgcg
     2221 acgcgcgaga tgctgtcaac caagggcccg gactggaaac cggagaccaa cgaggatttc
     2281 ggcgccctct tcgcccagcc tgtcctgatc gaatgcaagc gctgagccgg tttgccgcag
     2341 ccatcttcct ggggcttgcg cttctgcttg ccgcaacgcc cgccgcggtt cacgcgctgg
     2401 aagaggcggc cccggccgtc accatcgaca agcggaagct gctggtccag cctcgcaacg
     2461 ccgatggcag ccggcagcag gtctcgatct tcgaagaccc ggtgctgtgg gcccgcgaaa
     2521 agcagcagag cttctatggg cagatgtccg gtgccttgcg caacatgaag tcggggtcgg
     2581 cgcttgccgc cgcgtggacc ctgatgcttc tgagctttgg ctatggagtc ttccatgccg
     2641 ccgggcccgg ccacggcaag gccgtgatct cagcctggct gctggccacc gagaacgagt
     2701 tgcgccgtgg catcgccatt tccgcgatga gcgccgtgat ccaggccctg accgccatcc
     2761 tgatcgtcag catcttgctg ctacttgtcg cgagcgccag cgcaaaggcc cgcgacgttg
     2821 cgggtttcct cgaaagcgcc agctacgggc tcatcgggct catgggattg tacctgatct
     2881 ggaccgcctt ccgcagccgg agggcgagcc ctgcgccggc ccatgccggg cgcgatgcga
     2941 tgcaccgggt ctccgacctg catcacttcg acagcttccc gcccaagcag ccggcgcatc
     3001 ttccccatga tcatgtccat ggcccggatt gcggctgcgg ccatgcgcat ctccccgagg
     3061 cgcgcgacct gcgcggcgac tggtccttcg cccgggcttt ctcgctggcc tttgccgtgg
     3121 gaatccggcc ctgcaccggc gccattctgg ttctgatctt cgccaacgcg ctgggacttt
     3181 actgggccgg cattgccgcc accttcgcca tggccctcgg caccttcatc accgtctctg
     3241 ccatcgccgc catcgccgtc tactcgaaga agctggcgac gcgctatgcc cgtcaggacg
     3301 accgctggat cagctggctc aactttggct tgcgacttgg cggtggattg gtcatagcct
     3361 tcctgggatc gattctgttt ctggggtcgc tcggctcaac caacgccatg atgtagggga
     3421 cggcatgcgc aatcaatggc tggtactcgc cggtttcatc ttcgtctgtc tcgcggcggg
     3481 ctttggtgcg agcttcgcca ccgcgccggc gatcatcgaa tggtatccga ccctcaacaa
     3541 gccgtggtgg aacccgccca actgggtctt cggtccggcc tggacaattc tctacatcct
     3601 catggccatc gccgcatggc tggtctggag gcagccgaag gacaccaagg gcgcgctgat
     3661 cctgttcttc tcgcagcttg ccctgaattt cgcctggtcc ttcctcttct tcggcgcgcg
     3721 ctcgccctgg ttgggactga tcggcgtggc cttcatgctg gccgccgtgg cgattaccac
     3781 ggtggtgttc tggcgaatca cgaaacctgc gggaatgctc ttcattccct atcttgcctg
     3841 gaccgccttt gcggccttcc tcaacctgac catctggcgg atgaactgag cgggttccac
     3901 tgaccccgat cctgcgctag gctcccacca gggaaccgag cgatggccaa ggtctttttc
     3961 acggccaacc tgcgacgcca tgtcgactgc agcgccatgg aggccgatgg cgtgaccgtc
     4021 ggagacgtcc tttccacggt cttcgcaatt caggaccggc tcggcgccta tgtgctcgac
     4081 gaccagggcg ctttgcgcaa gcacatgacc atcctcgtcg atggccagcg gatcagggac
     4141 atcgagaggc ttaccgatcc tgtccggccg aacagcgaag tctgggttat gcaggccctc
     4201 tcgggagggt aatggaggac catcatgagc gagacgcttc tggtttccac acgaaaggga
     4261 ctcttcgaca tcggcatggg caagtcgggc tgggcggtga cggattcagc cttcctcggc
     4321 gacaatgtca cgctggcgct gcatgatcgg cgcgacggca cggactatgc cgcgctcaat
     4381 catggccatt tcggcgtgaa gctgcatcgc cgcgacaagg gtggcgtctg gacggagatt
     4441 gccgcccctg cctatccgcc caagcccgaa cgcctgaacg acgtcgatgg ctggggacgg
     4501 cccgtcaagt ggaccacgca gatgatctgg gcgctggagc cgggcggtgc cgacgagccc
     4561 ggaacgctct gggccggaac catgccgggc ggcctgttcc gttcgcgcga ccgcggcgaa
     4621 agctgggaga tgatgcgccc gctgtgggac atgccggaac gcaacaaatg gctgggcggc
     4681 ggcgccgaca ttccgggcat ccactcgatc tgcgtcgatc cccgctcatc gaagatcgtg
     4741 cgcgtggcaa tctcctgcgg cggcgtctgg gtcacccatg acggcggcga aagctgggcg
     4801 caatctgcgc atggcatgcg tttcgatgac ggccctgcag gcgtggccga cgacccgcat
     4861 acgcaggatc cgcacatgat ggtgcagtgc ccggccgcgc ccgaacgctt ctgggtgcag
     4921 caccattgcg gcatctggcg atcggcggac aacgcgatga gctggcagga ggtgaaggcc
     4981 aggccttcga gcttcggatt cggcgttgtc gtgcacccga aggacccgga tacggcctgg
     5041 ttcgtgccgg gcatcaagga cgagaagcgt tatccggtgg atggaaaact cgtcgtcacg
     5101 cgcacgcgcg acggcggaaa gagcttcgac atactcgaca aggggctgcc gggttcacat
     5161 gcctatgaca tcgtcttccg cc
//
LOCUS       DBNGVR010000425         3938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4477432, whole genome shotgun sequence.
ACCESSION   DBNGVR010000425 DBNGVR010000000
VERSION     DBNGVR010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4477432"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..791)
                     /locus_tag="AC16A0_09020"
     CDS             complement(195..791)
                     /locus_tag="AC16A0_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3105518.1"
                     /translation="MFYDAVANDHGLPQDPFKALVAPRPIGWISSLSADGIPNLAPFS
                     YFNAMAQAPHYVAFGIGPPKDTLRNVEVTGEFAVNLATYDLREAMNATSAHVAAHVDE
                     FELARLTRAPCRLIKAPRVGESPVCLECRLFKVGALPDDEGRENDHMVIGRVIGIHID
                     DRYIEDGRVNTAAMRPIARLGYGEYATVTEAWRMRRPA"
     gene            complement(781..1497)
                     /locus_tag="AC16A0_09025"
     CDS             complement(781..1497)
                     /locus_tag="AC16A0_09025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3105519.1"
                     /translation="MRIRGLLFDKDGTLIDVNRTWVPIYRHILADIFDVDEQGATAMM
                     EKVGYDAATGSFRANSIIAAGTTRQLAEAWWPELDAAGIAEKTRMLDNDYTHMVTAAL
                     HPLLPLDPLLAELRSMGMRLGVGTNDSHVSARSHMSHAGVLHHFDDIIGADTVPVAKP
                     SGQMVRRFADITGLPTSAIAMVGDNPHDMEEARKGEAGLAIAVLSGNAAHGDIAHLAD
                     YTIDSIADLPALLRSLQHVL"
     gene            complement(1497..2084)
                     /locus_tag="AC16A0_09030"
     CDS             complement(1497..2084)
                     /locus_tag="AC16A0_09030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3105520.1"
                     /translation="MSQLNDTSSILALLHSRKSASAKAMGEPGPRPDQLAEILRCAVR
                     VPDHGKLNPWRFIVFEGSARERFGAALRARWQELNPDHGESSLSFVQGMLLRAPTVIA
                     VVSTASDHPKIPVWEQVLSAGAVCQNMLLAATALGIGCQWNTDWIAYDTEVAKLMGLA
                     PHEKVAGLIYLGTPSVPLEDRPRPDPLALTTRWTG"
     gene            2250..3206
                     /locus_tag="AC16A0_09035"
     CDS             2250..3206
                     /locus_tag="AC16A0_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105521.1"
                     /translation="MHDFSTRAGHDRHHRRAARAAAVVAVLLFVAAITLLAPAIASSA
                     QPDSVEISGVPQAAEELFAEAMAEHDIAIEIVPPGREGLELTARLTDDGGLIERAISW
                     TILTPNGDTVYSGEAPEAAIATPPGDYAVSIRYGAVRLNSMVTLLEGNRLMVSYVLHA
                     GAIRILPRVQNIVAPGAAPDSRIYSLEGRQRGRLVAISTVPGEVVRVPEGLYRVESAF
                     DGGNARASTEVRVKAGRMSAVEIDHKAGVARLSYVGAPDAPVLWQLVDAKGQAVLAAE
                     GLSAHAVLLPGTYTASAQINGETLTATFGIAAGETRDIILGN"
     gene            3217..>3938
                     /locus_tag="AC16A0_09040"
     CDS             3217..>3938
                     /locus_tag="AC16A0_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3105522.1"
                     /translation="MTGRMKAAVLRTIGTPLKIETLPIPEPGPGEVLIKVHACGVCHS
                     DLHAVEGDWSPLPNLPLIPGHEVVGEVAALGAGVDHLAPGQRIGVPWMYSACGHCEFC
                     LSGMETICLAAEATGYSKPGGYAEYMVADAAFCGRIPDGADAYEIAPILCAGVTTYRG
                     LKRTGARPGQWVTVIGIGGLGHIAVQYARAMGLRVAAVDVSAAKLDHAKTLGAEIVVN
                     AAEVDPGTEIRRLIGGSHAAVVT"
BASE COUNT          710 a         1266 c         1235 g          727 t
ORIGIN      
        1 taacgaggta ttaatggtgt tatccacagc ttttccacaa gacctaacgc cctgtattga
       61 atgcattatc tgactattca ctggtgttta tcaacaatgg cggaattgcg gcgttccggc
      121 cctgttcacg actcgccacg cgactcaccg cggcgcacca ctaaacccag aattgcaacg
      181 tgtgatcgcc gcgcctatgc cgggcggcgc atgcgccagg cctctgtcac ggtggcgtat
      241 tcgccgtagc ccagcctggc gatcggacgc atggcggccg tattgacgcg cccgtcctcg
      301 atgtagcggt catcgatatg gatgccgatc acccgtccaa ttaccatgtg gtcgttctcc
      361 cgcccctcgt cgtccggcag cgccccgacc ttgaacaggc ggcattcgag acagaccggg
      421 ctttcaccca ccctcggtgc cttgatcagc cggcacggcg ccctggtcaa tcgcgccagt
      481 tcgaactcgt cgacatgcgc agcgacatgc gccgaggtgg cgttcatggc ttcacgtaga
      541 tcataggtgg caaggttgac tgcaaactcg ccggtcacct cgacattccg caacgtgtcc
      601 tttggtggac cgatgccgaa ggccacgtaa tgcggcgcct gcgccatggc attgaaataa
      661 ctgaatggcg ccagatttgg aatgccatcc gccgaaaggc tcgatatcca gccgatggga
      721 cgcggcgcca caagcgcctt gaacggatcc tgcggcagac cgtggtcatt cgccaccgcg
      781 tcatagaaca tgttgcaggc tccgcaacag ggccggcagg tcggcgatgc tgtcgatggt
      841 atagtcagcc agatgggcaa tgtcgccatg ggctgcattg ccggacaaga cagcaatggc
      901 cagtcccgcc tcgcctttgc gggcctcttc catgtcatgc ggattgtcgc ccaccatggc
      961 gatcgcggat gtgggcaatc ccgtgatgtc ggcaaaacgc cggaccatct ggcccgatgg
     1021 cttggccacc ggcaccgtat cggcgccgat gatgtcatcg aaatgatgca gcacgccggc
     1081 gtggctcatg tggctgcgcg cagagacatg gctgtcgttg gttcccacgc ccagccgcat
     1141 gcccattgac cggagttcgg caagcaacgg atcgagtggc aacagcggat gcagcgcggc
     1201 cgtcaccata tgcgtatagt cattgtccag catccgggtc ttttcggcga tgccggcggc
     1261 gtcgagttcc ggccaccacg cctccgccaa ttgccgcgtg gttccggcag cgatgatcga
     1321 attggcgcga aacgagcccg tcgcagcgtc atagcccacc ttttccatca tggccgtggc
     1381 gccttgctcg tcgacatcga agatgtctgc cagaatatgc cggtagatcg gcacccaggt
     1441 gcggttcacg tcgatgaggg tgccgtcctt gtcgaacaac aggccgcgga tgcgcatcag
     1501 ccggtccaac gcgtggtcag cgccaaggga tccggccggg gccggtcttc cagcggcacg
     1561 gagggcgttc ccagatagat caggccggcc accttctcgt gcggggcaag gcccatcaac
     1621 tttgcgactt cggtgtcata ggcaatccag tcggtgttcc attgacagcc aatcccgagt
     1681 gccgtcgctg ccagcagcat attctggcat acggcgccgg ccgaaagcac ctgctcccag
     1741 accgggattt tcggatggtc cgaggcggtc gagacgacgg cgatcaccgt cggggcgcgc
     1801 agcagcatgc cctggacgaa ggacagcgag ctttccccgt gatcgggatt gagttcctgc
     1861 caacgcgctc tcagggccgc accgaagcgt tcgcgtgccg atccctcgaa gacgatgaag
     1921 cgccagggat tgagcttgcc gtgatcgggc acccgcaccg cgcagcggag gatttcggcc
     1981 aattgatccg gacgagggcc tggctcaccc atcgccttgg ccgaggcaga cttgcgggag
     2041 tgaagaaggg ccagaatgct ggaggtatcg ttgagttgcg acatggtccc ggcaatgtgg
     2101 cgggggcatt gcgaaaaggc aatggtttca attgcggcaa ccccattaac cccaaattaa
     2161 ggatgccgtt gccgccctgg ggccggcgtg gttgcttgta tgggccgacc ggactggtcg
     2221 ggggagttgg gacgaagggg actgcaaact tgcacgactt cagcacccgt gcaggccatg
     2281 accgccatca ccgccgcgcc gcaagggccg cagcagtggt ggcggttctt cttttcgtcg
     2341 cagcgatcac cctgcttgcg ccggccatcg cctcctccgc gcagcccgac tcagtcgaga
     2401 tctcgggcgt tcctcaggcc gccgaggagc tcttcgccga agccatggcc gaacatgaca
     2461 tcgcgatcga gatcgtaccc cccggaaggg agggcctgga actgacggct cgcctgaccg
     2521 atgacggcgg cctcatcgag cgcgccattt cctggaccat cttgacgccc aatggagata
     2581 ccgtttactc cggcgaggca cccgaagcag ccatagcgac accgcccggc gactatgccg
     2641 tctccatccg atacggtgcg gtgcggctca acagcatggt gacactgctt gagggcaacc
     2701 ggctgatggt gagctacgtg ctccacgctg gcgccatccg cattcttccg cgcgttcaga
     2761 acattgttgc ccccggtgcc gcgcccgaca gccgcatcta ctcgctcgaa ggccgccagc
     2821 gcggcaggct tgtcgcgatc agtaccgtgc caggcgaagt cgtccgcgtt ccggagggcc
     2881 tctaccgggt cgaaagcgcc ttcgacggag gcaatgccag ggcctcgacg gaggtgcggg
     2941 tgaaggccgg ccgcatgagc gcagtggaga tcgatcacaa ggcgggtgta gcccgtcttt
     3001 cctatgtcgg cgccccggac gctcctgtcc tgtggcagct tgtcgacgcc aaggggcaag
     3061 cggtgcttgc cgccgaaggc ttgagcgccc atgccgtgct gctgcccgga acctataccg
     3121 ccagcgccca gatcaatggc gagaccctga ccgccacttt cggcattgct gcaggcgaaa
     3181 cgcgcgatat catcctcgga aactgaggaa cgccccatga ccggtaggat gaaggctgcg
     3241 gtactccgca cgatcggcac gcctctgaaa atcgaaactc tgccgatacc cgagccaggc
     3301 cccggcgaag tcctgatcaa ggttcacgcc tgcggcgtct gtcactccga tcttcatgcg
     3361 gtcgaaggag actggtcgcc gctgccgaac cttccgctga tccctggtca cgaggtggtg
     3421 ggtgaagttg cggctctggg tgccggcgtc gatcacctcg cgccgggcca gcggatcggc
     3481 gttccctgga tgtattcggc ctgcggccac tgcgaattct gcctgtcggg gatggaaacg
     3541 atctgcctcg ccgccgaagc gacgggctat tcgaagcccg gcggctatgc cgaatacatg
     3601 gtcgccgacg cggccttctg cgggcggatc cccgatggcg ctgacgccta tgagatcgca
     3661 cccatcctct gcgccggtgt gaccacctat cgcggcctga agcgcaccgg tgcccggcct
     3721 ggccagtggg tgaccgtgat cggcatcggc ggcttggggc acattgccgt gcaatatgcc
     3781 cgcgccatgg gactgcgtgt cgctgccgtg gatgtctccg ccgccaaact cgaccatgcg
     3841 aagactcttg gcgccgagat cgtcgtcaat gccgccgagg tcgatcctgg cacggagatc
     3901 aggcggctca tcggcggcag ccatgccgcg gtcgtcac
//
LOCUS       DBNGVR010000426         2348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4674270, whole genome shotgun sequence.
ACCESSION   DBNGVR010000426 DBNGVR010000000
VERSION     DBNGVR010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4674270"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..>2348
                     /locus_tag="AC16A0_09045"
     CDS             25..>2348
                     /locus_tag="AC16A0_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR3105523.1"
                     /translation="MTALPDKAQFVVIGGGIIGCSTAYHLARDHKADVVLLEQNRLTS
                     GSTWHAAGLVGQLRSSASITQVLKYSVDLYKKLDAETGLETGWKMTGCLRLATNKDRW
                     TEYKRLATTAQSFGMEMHLLSAAEVKTMWPLMETSDLVGASWLPTDGQASPSDISQSL
                     AKGARMHGAKIVEGARVTGFEIANGRVVAVETNLGRIACETVVNCGGQWARQIGAMAG
                     VSVPLQAVKHQYVITEKVDGLASDAATLRDPDRRTYFKEEVGGLVFGGYEPNPIAWTQ
                     GDVPDDFEFQLFEDDWDHFEQHMGQALARVPALEKAGIKKMINGPESFTPDGNFILGA
                     APELRNFFVGAGFNAFGIASGGGAGWVLAEWAAKGEAPMDLWGVDIRRFSDLHKDRQW
                     TCDRTLEAYGKHYTIGFPHEEYQSGRPRIVSPLYDRLKKLNACFGSKLGWERPNWFAP
                     RGVEPRDVYSMGRQNWFDAVGAEHRAVREAAGLFDQSSFAKYELRGRDAGEALSMICA
                     NRVVRGAGRLTYTQMLNSRGGIECDLTVAQLSDDLFYIVTGTGFRTHDMAWIADHIKP
                     GLDARLTDVTESWGTLSLMGPKARDILAKVTTSDVGNAAFPFGTVREIEIAGCRLRAL
                     RVTYVGELGWELHVPIAAIGEVFDALTSAGTPLGLRPAGYRALEALRLEKGYRAWGSD
                     ITPNDSPFHAGLGWAVKLKSNQDFIGRSAVERIAATPLTKMLAGFTTERDDIVLVGRE
                     TILRDGEFAGYLTSGGYGYTIGKPIGYGYVRNGAG"
BASE COUNT          409 a          743 c          786 g          410 t
ORIGIN      
        1 ctatctcagc aacttcggaa aatcatgaca gctcttcccg acaaggccca gttcgtcgtc
       61 attggcggcg gcatcatcgg ctgctcgacg gcctatcatc tggcgcgcga ccacaaggcg
      121 gatgtggtcc tgcttgagca gaacaggctg acatccggtt cgacatggca tgcggccggc
      181 cttgtgggcc agctccgctc ctcggcctcc atcacgcagg tgctgaagta ttccgtcgat
      241 ctctacaaga aactcgacgc cgagacggga ctcgagacgg gctggaagat gaccggctgt
      301 ctccgccttg ccaccaacaa ggaccgctgg accgagtaca agcggctggc cacgacggcc
      361 cagagcttcg gcatggagat gcatctcctg tctgctgccg aggtcaagac gatgtggccg
      421 ttgatggaga cgtccgatct cgttggagcc tcctggctgc ccaccgacgg ccaggcctcg
      481 ccctcagaca tctcgcagtc gctggccaag ggtgcgcgca tgcatggcgc gaagatcgtc
      541 gaaggcgcac gggtgaccgg tttcgagatc gccaatggcc gggtcgtggc ggtggagacc
      601 aatctggggc gcattgcctg cgagacggtg gtcaattgcg gcggacaatg ggcgcggcag
      661 atcggcgcca tggccggcgt gtcggtgccg ctgcaggcgg tcaagcacca gtatgtcatc
      721 accgagaagg tggatggcct tgcgtccgat gcggcgacgc tgcgcgatcc cgaccgccgc
      781 acctacttca aggaagaggt gggcgggctc gtcttcggcg gctacgagcc gaatcccatt
      841 gcctggacgc agggcgacgt gccggacgat ttcgagttcc agctcttcga agacgactgg
      901 gatcacttcg aacagcacat gggccaggcc ctggcgcgcg tgcccgcgct ggagaaggcc
      961 ggcatcaaga agatgatcaa tggccctgag agctttacgc cggacggaaa cttcatcctt
     1021 ggtgcggcgc ccgagctccg caacttcttt gtcggtgccg ggttcaacgc cttcggcatc
     1081 gcctcgggcg gtggcgcagg ctgggtgctt gcggaatggg cagccaaggg cgaggcgccc
     1141 atggatctct ggggggtcga tatccggcgc ttctcggacc tccacaagga ccggcagtgg
     1201 acctgcgacc gtaccctcga ggcctatggc aagcactaca ccatcggatt cccgcacgag
     1261 gaataccaga gcggccgtcc gcgcatcgtc tcgccgctct atgatcgcct gaagaaactc
     1321 aatgcctgct tcggatcgaa gctcggctgg gaacggccca actggttcgc gcccagaggc
     1381 gtcgagccgc gcgacgtcta ttcgatgggc cggcagaact ggttcgacgc cgtgggagcg
     1441 gagcatcggg cggtgcgcga ggcggcggga ctcttcgacc agtcctcctt cgcgaagtat
     1501 gaactcaggg gacgcgatgc cggcgaggcc ctgtccatga tctgcgccaa tcgtgttgtg
     1561 cgcggcgcgg gacggctgac ctatacgcag atgctcaaca gccgcggggg catcgagtgc
     1621 gatctcacgg tggcccagct ttccgacgat ctgttctaca tcgtcaccgg caccggtttc
     1681 cggacgcatg acatggcgtg gatcgcggat cacatcaagc caggtctcga tgcgcgactg
     1741 acggatgtga cggaatcctg gggcacgctc tcgctgatgg ggccgaaagc gcgcgacatc
     1801 ctcgcgaagg tgaccacatc ggacgtcggg aacgccgcct ttcccttcgg caccgtgcgc
     1861 gagatcgaga tcgccggctg ccggctgcgc gcgctgcgcg tcacctatgt cggcgaactg
     1921 ggctgggagc tgcatgtccc catcgccgcc attggggaag tcttcgatgc gctgacgtcc
     1981 gccggcacgc cgctgggcct gaggccggcc ggctatcggg cgctcgaggc gctgcggctg
     2041 gagaagggct atcgcgcctg gggttccgac atcacgccca acgactcgcc cttccatgcg
     2101 gggcttggct gggccgtgaa gctcaagtcc aaccaggact tcattggccg gtccgccgtc
     2161 gaacggattg cggcgacccc gctgaccaag atgctggccg gtttcacgac cgagcgcgac
     2221 gacatcgtgc tcgtcggccg ggagacgatc ctgcgcgacg gtgagttcgc cggctatctc
     2281 accagcggcg gctacggcta caccatcggc aagccgatcg gctacggcta tgtgcgcaat
     2341 ggcgcggg
//
LOCUS       DBNGVR010000427         6986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4086051, whole genome shotgun sequence.
ACCESSION   DBNGVR010000427 DBNGVR010000000
VERSION     DBNGVR010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4086051"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2713
                     /locus_tag="AC16A0_09050"
     CDS             <1..2713
                     /locus_tag="AC16A0_09050"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3105524.1"
                     /translation="NWPAAPAPAAKSGAKPADKAPGLSPEDVRKATMDSVRALMMIRA
                     YRIRGHLAADLDPLRLKAPIAHPELDPASYGFTEADMDRPIFLDKVLGLEHATLRQIV
                     DILRRTYCGTLGVEFMHISDPEQKGWIQERIEGADKPIAFTPKGKKAILAKLISAEGF
                     ENFLNVKYTGTKRFGLDGGESMIPALEQIIKRGGQLGLQEIVLGMAHRGRLNVLANVM
                     SKPFSAIFHEFKGGSSTPDEVEGSGDVKYHLGSSSDRAFDGNTVHLSLTANPSHLEIV
                     DPVVLGKARAKQDQRKDKDRTSVLPLLIHGDAAFAGQGVVAECFGLSGLRGHRSGGSI
                     HFIVNNQIGFTTAPHWSRSSPYPSDVAKMIEAPIFHCNGDDPEAVVYAAKIAIEFRQK
                     FKKPVVIDMFCYRRFGHNETDEPAFTQPLMYKRIGGHQTVVALYGKRLVDEGVITAAE
                     LDEMKAEYRKTLDDDFTVAEGYKANKADWLDGRWAGLKAARDHEDPRRGQTGVAVDKL
                     KEMGLKLTKVPKTFKVHRTMQRVLDARRKMIEDGSGLDWSMAEQLAFGTLLMEGFPVR
                     LSGQDVQRGTFSQRHAVLIDQETEKRYTPLNHLLKGQEVKLEAINSMLSEEAVLGFEY
                     GYSLAEPNALTIWEAQFGDFANGAQVVFDQFISSGERKWLRMSALVCLLPHGYEGQGP
                     EHSSARLERFLQMCAEDNMQVANCTTPANYFHILRRQLKRDFRKPLVIMTPKSLLRHK
                     RATSRLDELGEGTSFHRILWDDAQLLEGEKIKLKPDDKIRRVIMCSGKVYYDLYEERE
                     KRGIDDIYLLRVEQLYPWPHKVLIQELARFENAEFVWCQEEPYNMGAWSFVQPNIERV
                     LEFNGARNARVRYVGRAASAATATGLMSKHLRELKAFLDDAFGDSKA"
     gene            2726..3946
                     /gene="odhB"
                     /locus_tag="AC16A0_09055"
     CDS             2726..3946
                     /gene="odhB"
                     /locus_tag="AC16A0_09055"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR3105525.1"
                     /translation="MAKEIRVPALGESVTEATIAKWFKQAGEAVKADEPLVELETDKV
                     TVEVPAPASGKLLAIDAAAGSTVNVGALLGSIEEGAAGGVAAKPAEAPARKPDPAPAA
                     AKAEQALSPAVRRMVTENKIDAAAITGTGKDGRVTKGDVIAALEKPAADIKPLVIPAA
                     PAPAARAPSAPSEAAREERVRMSRLRQTIARRLKDAQNVAAMLTTFNEVDMTATMALR
                     NQYKEVFEKKHGVKLGFMSFFVRACVQALKEIPAVNAEIDGEDIVYKNYYNIGVAVGT
                     DKGLVVPVLRDADMLSFAGIEKTIAEYGKRARDGQLQISDMQGGTFTISNGGVYGSLM
                     STPILNAPQSGILGMHKIQDRPVALNGQVVIRPMMYLALSYDHRIVDGKDAVTFLVRV
                     KENLEDPQRALLDL"
     gene            3950..4702
                     /locus_tag="AC16A0_09060"
     CDS             3950..4702
                     /locus_tag="AC16A0_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3105526.1"
                     /translation="MTGKGVAIVTGGSRGIGRATALKLGQAGYTVVVNYVSKPAAADA
                     VVDQIRKAGGMAAAVAGDVAREADVLALFAAADRLGPLTVLINNAGVMDSQARLDEMS
                     LDRLTRLFNINILGSFLCAREAVKRMSTAHGGKGGVIVNQSSAAAVLGAPALGVEYAA
                     SKGAIDVLTIGLGREVAAEGIRVNAVRPGIIDTEIHDSSGIPGRVQKMRDVIPMKREG
                     KAEEVADVIMWLVSDQSSYVTGSIVPVAGGRC"
     gene            4723..6111
                     /gene="lpdA"
                     /locus_tag="AC16A0_09065"
     CDS             4723..6111
                     /gene="lpdA"
                     /locus_tag="AC16A0_09065"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3105527.1"
                     /translation="MANQFDVTIIGTGPGGYVCAIRAAQLGLTVAVVEKRATHGGTCL
                     NVGCIPSKALLHASEMYHEAEHVFSKMGIEAKPNLDFAQMMIFKQEAIDGNTKGIDFL
                     LKKNKIKFFQGAGVIEAAGRVRVGKEPIESKHIVIATGSDVARLKGIEIDEQQIVSST
                     GAISLAKVPETLAVIGAGVIGLELGSVYARLGSKVTVIEYLDRILPGMDGDVAKAFQR
                     ILQKQGFTFKLASKVTGASKAKGKVTLTVEPAAGGACEPLEADVVLVAVGRVPYTEGL
                     GLDAVGVKRDARGRVEVDHHYATSVPGIFAIGDVIRGPMLAHKAEDEGIALAEILAGQ
                     AGHVNYGVIPGVVYTSPEVAAVGQTEEDLKSAGIDYKAGKFLFLANGRAKANQTTDGF
                     VKVLADARTDRVLGCHIVGPMAGELIHEVAVLMEFGGSAEDLARTCHAHPTLSEAVKE
                     AALAVAGRAIHA"
     gene            6174..6572
                     /locus_tag="AC16A0_09070"
     CDS             6174..6572
                     /locus_tag="AC16A0_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3105528.1"
                     /translation="MAEASKQVDILVLDDDVDLLSSVADLLADCGYRVKAFADADSAI
                     DFAMGQSFAVGLFDYRLGSLKNGLDVVEKMQALGCESVYVMITADVEQATQMRAIHLK
                     LFEFMRKPVQPDKLLETVQRALDHAHRAAA"
     gene            complement(6579..>6986)
                     /locus_tag="AC16A0_09075"
     CDS             complement(6579..>6986)
                     /locus_tag="AC16A0_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105529.1"
                     /translation="PGSHHWAPINVPYVNIGDWEPQPLTPEEEAGIPKDLSAPYPGEV
                     LVEAPAGSAIICNSSMWHAGTLKKTDAPRRMLHLTYTRRDLPQQLLQLDYLTPELYNR
                     MSPVKRYLMEIEPPKPGDGILRQPKKDHKGWWN"
BASE COUNT         1353 a         2117 c         2268 g         1248 t
ORIGIN      
        1 caattggccg gcggcgccgg cgcctgccgc caagtctggc gcgaaacccg ccgataaggc
       61 gcccggcctg tcgcccgaag acgtgcgcaa ggccaccatg gactcggtcc gtgcgctgat
      121 gatgatccgc gcctaccgga tccgcggcca tcttgccgcc gacctcgatc ccttgcggct
      181 caaggctccc atcgcccacc cggaacttga tccggcctcc tacggcttca ccgaggccga
      241 catggaccgg ccgatcttcc tcgacaaggt gctcggcctc gaacatgcca cgctgcggca
      301 gatcgtcgat atcctgcggc gcacctattg cggcacgctc ggcgtcgaat tcatgcatat
      361 ctcggatccc gagcagaagg ggtggatcca ggagcgcatc gagggtgccg acaagccgat
      421 cgccttcacg cccaagggca agaaggcgat tcttgccaag ctgatctctg ccgagggttt
      481 cgagaacttc ctcaacgtga aatacaccgg caccaagcgg ttcggcctcg acggtggcga
      541 gtccatgatt ccggcgctgg aacagatcat caagcgcggc ggccagcttg ggctgcagga
      601 gatcgtgctg ggcatggccc atcgcggccg cctcaacgtt ctggccaacg tgatgtccaa
      661 gcccttctcg gccatcttcc atgaattcaa gggcggatcc tcgaccccgg acgaggtcga
      721 aggctcaggc gacgtcaagt atcacctcgg ctcttcgtcg gaccgggcct tcgacggcaa
      781 tacggtccat ctctcgctga ccgccaatcc gtcgcatctc gagatcgtcg atccggtggt
      841 gctcggcaag gcgcgggcca agcaggacca gcgcaaggac aaggaccgca ccagcgtgct
      901 gccgctgctg atccacggcg atgccgcctt cgccggccag ggcgtcgtgg ccgagtgctt
      961 cgggctctcg ggcctgcgtg gccaccggtc gggcggctcg atccatttca tcgtcaacaa
     1021 ccagatcggc ttcaccacgg cgccgcactg gtcgcgatcc tcgccttatc cctccgatgt
     1081 cgccaagatg atcgaggcgc cgatcttcca ttgcaatggt gacgacccgg aagcggtggt
     1141 ctatgccgcc aagatcgcca tcgagttccg gcagaagttc aagaaaccgg tggtgatcga
     1201 catgttctgt taccgccgtt tcggacacaa cgagacggat gaaccggcct tcacgcagcc
     1261 gctgatgtac aagcgcatcg gtggccacca gacggtggtg gcgctctatg gcaagaggct
     1321 ggtcgacgag ggcgtgatca cggcggccga actcgacgag atgaaggccg aataccgcaa
     1381 gacactggat gacgacttca ccgtggccga aggttacaag gccaacaagg ccgactggct
     1441 cgacggccgc tgggcgggcc tgaaagcggc acgcgatcat gaggatccgc gccgcggcca
     1501 gaccggcgtt gccgtcgaca aactcaagga gatgggcctc aagctcacca aggtgcccaa
     1561 gaccttcaag gtccaccgca ccatgcagcg cgtgctggac gcgcgccgca agatgatcga
     1621 ggacggcagc gggctcgact ggtcgatggc cgagcaactg gctttcggca cgctgctgat
     1681 ggaaggcttt cccgtgcgcc tgtcgggtca ggacgttcag cgcggcacct tttctcagcg
     1741 ccacgccgtc ctgatcgatc aggaaaccga gaagcgctac acgccgctca atcacctgct
     1801 caaggggcag gaggtgaagc tcgaggcgat caattccatg ctgtcggaag aggccgtgct
     1861 tggcttcgag tatggctatt cgctggcaga gcccaatgcg ttgaccatct gggaggcgca
     1921 gttcggcgat tttgccaatg gcgcgcaggt ggtcttcgac cagttcattt ccagtggcga
     1981 gcgcaagtgg ctgcgcatgt cggccctggt ctgcctgctg ccgcatggct atgaggggca
     2041 agggccagag cattcctccg cccggctcga gcgcttcctc cagatgtgcg cggaagacaa
     2101 catgcaggtc gccaactgca caacgccggc caattatttt cacatcctgc ggcgccagct
     2161 gaagcgcgac ttccgcaagc cgctggtcat catgacaccc aagtcgctgc tgcgccacaa
     2221 gcgggccacc tcgcgtctcg acgagctggg cgagggcacg agcttccacc gcatcctgtg
     2281 ggatgacgct cagcttctcg agggtgagaa gatcaagctc aagccggatg acaagatccg
     2341 gcgtgtcatc atgtgcagcg gcaaggtcta ctacgacctt tacgaggagc gcgagaagcg
     2401 gggaattgac gacatctacc tgctgcgcgt ggagcagctc tatccctggc cgcacaaggt
     2461 tctgattcag gaactggcgc gcttcgagaa tgccgagttc gtgtggtgcc aggaagaacc
     2521 ctacaacatg ggcgcgtgga gcttcgttca gccgaacatc gagcgcgtgc tggaattcaa
     2581 cggcgccagg aacgcgcgcg tccgctatgt cggccgggcc gcatcggcag ccaccgcaac
     2641 gggactcatg agcaagcatc tcagggagct caaggcattc ctcgacgatg ccttcggcga
     2701 cagcaaggca taagggacag acaagatggc gaaggaaatc cgcgttccgg ctctcggcga
     2761 atcggttacc gaagcgacga tcgccaagtg gttcaagcaa gccggtgaag cggtgaaggc
     2821 cgacgagccg ctggtggaac tggagaccga caaggtcacg gtggaagtgc cggcgcctgc
     2881 gtcgggcaag cttctggcga tcgatgcggc ggccggcagc accgtcaatg tcggcgcact
     2941 tctggggtcg atcgaggaag gtgcggctgg gggtgttgct gcaaagcccg ccgaagcgcc
     3001 ggcccgtaag ccagatccgg cgcctgcggc ggccaaggcc gagcaggcct tgtcgccggc
     3061 cgtgcgcagg atggtgacgg agaacaagat cgacgccgct gccatcaccg gcaccggcaa
     3121 ggatggccgg gtcaccaagg gcgacgtcat cgcagcgctg gaaaagcccg ctgcggacat
     3181 caagcccctg gtcattcccg cggcaccggc acctgcggcg agggcgccat ccgctccgtc
     3241 ggaggccgca cgtgaagagc gggttcgcat gtcgcgcctg cgccagacga tcgcccggcg
     3301 cctgaaggat gcgcagaacg tcgccgcaat gctgaccacc ttcaatgagg ttgacatgac
     3361 ggccaccatg gcgctgcgca accagtacaa ggaggtcttc gagaagaagc acggggtgaa
     3421 gctgggcttc atgagcttct tcgtaagggc ctgcgtgcag gccctgaagg agattcccgc
     3481 cgtcaatgcc gagatcgatg gcgaggacat cgtctacaag aactactaca acatcggcgt
     3541 ggcggtgggg accgacaagg gcctggtcgt tccggtgctg cgcgatgccg atatgctgtc
     3601 cttcgccgga atcgagaaga ccatcgcgga atatgggaag cgtgcgcggg acggtcagct
     3661 gcagatctcc gacatgcagg gtggaacctt cacgatctcg aacggcggtg tctatggctc
     3721 gctgatgtcg acgccgattc tcaatgcacc gcaatcgggc attctcggca tgcacaagat
     3781 ccaggaccga cccgtcgccc tgaacggcca ggtggtgatc cgcccgatga tgtatctggc
     3841 gctttcctac gatcaccgta tcgtcgacgg caaggatgcg gtcacgttcc tggtgcgggt
     3901 gaaggagaat ctcgaggacc cgcagcgtgc gctgctggat ctctgagcca tgaccggcaa
     3961 gggcgttgcc atcgtcaccg gcggaagccg cggcatcggc cgggcgacag cgttgaagct
     4021 gggccaggcc ggctacacgg ttgtggtgaa ctacgtgtcg aagcctgcgg cggcggatgc
     4081 ggtcgtggac cagatcagga aggccggcgg aatggccgcc gccgttgccg gcgacgtggc
     4141 ccgggaggcc gatgtgctgg cgctctttgc agccgccgac cggctggggc cgctcacggt
     4201 gctgatcaac aatgccggag tcatggattc gcaagcccgg ctggacgaga tgagcctcga
     4261 tcgcctgaca cggctcttca acatcaacat cctggggtct ttcctctgtg cccgtgaagc
     4321 ggtgaagcgc atgtcgaccg cgcatggcgg caaggggggc gtgatcgtca atcagtcgtc
     4381 ggcggcagcg gtgctgggtg ctcccgcgct gggcgtggaa tatgctgcca gcaagggggc
     4441 catcgatgtc ctgacgatcg gactcggacg cgaagtggcg gccgagggca tccgggtcaa
     4501 tgccgtcagg cccgggatca tcgataccga gattcacgac agttccggca ttcccggcag
     4561 ggttcagaag atgcgcgacg tgatccccat gaagcgcgag ggaaaggccg aggaagtggc
     4621 ggacgtcatc atgtggctgg tctccgacca gtccagctat gtgacaggaa gcatcgttcc
     4681 agtggccggc gggcgctgct gaaacacgac agggacagga ccatggcaaa ccagtttgac
     4741 gtcaccatca tcgggaccgg gcctggaggc tatgtctgtg ccatccgggc ggcacagctg
     4801 gggttgaccg tggcggtggt ggagaagcgg gcgacccatg gcggcacctg cctcaatgtc
     4861 ggttgcattc cgtcgaaggc tctgttgcac gcctccgaga tgtatcacga agccgaacat
     4921 gtgttctcga agatgggtat cgaagcgaaa ccaaatctag attttgcgca gatgatgata
     4981 ttcaagcagg aggcgatcga cggcaatacg aagggcatcg attttctcct taagaagaac
     5041 aagatcaagt tttttcaagg cgccggagtg atcgaagccg ctggtcgcgt gcgtgtcgga
     5101 aaagaaccta tcgaatcgaa acatattgtc attgcgacag gttccgatgt ggccaggctc
     5161 aagggtatcg agattgacga gcagcagatc gtctcgtcca ccggcgcgat atcccttgcc
     5221 aaggtccccg aaacgcttgc ggtgattgga gcgggggtca tcgggcttga gctcggttcc
     5281 gtctatgcaa ggctggggtc caaggtgact gtcatcgagt atctcgaccg gatcctgccg
     5341 ggcatggatg gcgatgtcgc caaggccttc cagcgcatcc tgcagaaaca gggctttacc
     5401 ttcaagctgg cttcgaaggt caccggcgcc agcaaggcca aaggcaaggt cacattgacg
     5461 gtggagcctg ccgcaggcgg ggcctgcgaa ccactcgagg cagatgtggt gctcgtggcg
     5521 gtcgggcggg tgccctatac cgaaggactc gggctcgacg cagtcggcgt caagcgcgac
     5581 gccaggggcc gtgtggaggt tgaccaccac tatgccacca gcgtccccgg catttttgcc
     5641 atcggcgatg tcatccgggg gcctatgctg gcgcacaagg ccgaggacga gggcattgcc
     5701 cttgccgaga tcctcgcggg ccaggccggc cacgtcaact acggggtgat ccccggcgtt
     5761 gtctatacaa gtccggaggt cgccgcagtg ggccagaccg aagaggacct gaagtccgcg
     5821 ggcatcgact acaaggcagg gaagttcctg ttcctcgcca atggccgggc caaggccaac
     5881 cagaccaccg acggcttcgt gaaggtcctg gccgatgcca ggacggaccg ggtgctggga
     5941 tgccacatcg tcggcccgat ggccggcgaa ctcatccatg aggtggcggt gctgatggag
     6001 ttcggcggct cggcagagga tctcgcccgt acctgccacg cccatccgac cctgtcggag
     6061 gccgtgaagg aagcggctct ggccgtggcc ggtcgcgcca ttcacgccta atgcattaac
     6121 gcaagctaca cccgccgctg ctagcctccc gtgcagttca caagggatgt gccatggctg
     6181 aggcttccaa acaggtcgat attcttgttt tagatgatga tgtcgatcta ctttccagcg
     6241 tggccgatct gctggctgat tgcggttacc gtgtgaaagc ctttgccgat gccgactcgg
     6301 ccatcgactt cgcgatgggt cagagtttcg ccgtgggcct cttcgattat cgtcttggca
     6361 gcttgaagaa cggtctcgac gtcgtggaga agatgcaggc gctcggctgc gagtcggtct
     6421 atgtgatgat cactgccgac gtcgagcagg ccacgcagat gcgcgccatc catctcaagc
     6481 tcttcgaatt catgcgcaag cccgtgcagc ccgacaagct tctcgagacc gtgcagcggg
     6541 ccctcgatca cgcccaccgc gccgcagcct gacaccgatc aattccacca gcccttgtgg
     6601 tccttcttgg gctgacggag gattccgtcc ccgggcttcg gcggttcgat ctccatcaga
     6661 tagcgcttca ccgggctcat gcgattgtaa agctccggtg tgagataatc gagctgcagg
     6721 agctgttgcg gcaggtcacg ccgcgtatag gtaagatgaa gcatgcgccg tggcgcgtcc
     6781 gtcttcttca acgtgccggc atgccacatg gatgaattgc agatgattgc cgagcctgcc
     6841 ggcgcttcca ccagcacttc tcccggatag ggtgcgctca ggtccttcgg aatgccggcc
     6901 tcttcctcgg gcgtgagcgg ttgaggctcc cagtcgccaa tgttcacgta gggcacattg
     6961 atcggcgccc agtggtgcga accggg
//
LOCUS       DBNGVR010000428         4696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5263831, whole genome shotgun sequence.
ACCESSION   DBNGVR010000428 DBNGVR010000000
VERSION     DBNGVR010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5263831"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..826)
                     /locus_tag="AC16A0_09080"
     CDS             complement(110..826)
                     /locus_tag="AC16A0_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR3105530.1"
                     /translation="MTALLFREDSQLRSCTATVSAIDERGGILLDRTVFYATAGGQPG
                     DKGRIAWDGGEISIATTIYDAAKNVVHVPEPNCATPAVGATVMATLDWDNRHRNMRAH
                     TLMHLLCASVPFPVTGGAITEDGGRIDFDIPEGEIPDKAALSERINRLIAEDHPVSYR
                     WITDAEMEENQHLIRTMSVKPPMGTGRVRLVLIGENGKIDLQPCGGTHLQSTGGIGPV
                     AVGKIENKGRINRRIRVVFA"
     gene            complement(823..1848)
                     /locus_tag="AC16A0_09085"
     CDS             complement(823..1848)
                     /locus_tag="AC16A0_09085"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR3105531.1"
                     /translation="MDIKQDVIAAIGNTPLIKLRKASELTGCTILGKAEFMNPGQSVK
                     DRAALFIIRDAIARGSLKPGGTIVEGTAGNTGIGLTVVANAMGFRSVIVIPVTQSQEK
                     KDALRQLGAELVEVPAVPYKNPNNYVKYSGRLAKAMGAVWANQFDNTANRLAHVETTA
                     PEIWAQTDGKVDAFVAAVGSGGTLAGVSMGLKSRNPAIKIALADPMGAALYNYYKFGE
                     LKSEGSSITEGIGQGRITANLEGAIVDEAFQIPDSEAIPIAFDLLEHEGLCMGGSTGV
                     NVAGAIRMAKALGPGHTIVTILCDYGTRYASKLFNPTFLREKNLPVPTWLEAVPRVPD
                     VTEEVPA"
     gene            complement(1967..2042)
                     /locus_tag="AC16A0_09090"
     tRNA            complement(1967..2042)
                     /locus_tag="AC16A0_09090"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2007..2009),aa:Asn,seq:gtt)
     gene            2194..2427
                     /locus_tag="AC16A0_09095"
                     /pseudo
     CDS             2194..2427
                     /locus_tag="AC16A0_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018445659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2434..3030)
                     /gene="bchJ"
                     /locus_tag="AC16A0_09100"
     CDS             complement(2434..3030)
                     /gene="bchJ"
                     /locus_tag="AC16A0_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriochlorophyll 4-vinyl reductase"
                     /protein_id="HIR3105532.1"
                     /translation="MSQAVAALIGPNAVIQVAKALDAVEGRSASTRIFGEAGIAHYLE
                     RPPETMIAESDVVELHHRLRAGLGERHSASVSWVAGHHTANYLLANRIPAPVQHLLRR
                     LPAPLAGRILLQAISRHAWTFTGSGRFTYIGFKPVHFSIEDCPLCRNATSQSPCCTYY
                     AATFERLFRELVSADAHVTETECAGMGAKACRFRIDWA"
     gene            complement(3037..4647)
                     /gene="bchE"
                     /locus_tag="AC16A0_09105"
     CDS             complement(3037..4647)
                     /gene="bchE"
                     /locus_tag="AC16A0_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     anaerobic oxidative cyclase"
                     /protein_id="HIR3105533.1"
                     /translation="MKVVLIHPNHHSGGAEIAGNWPPAWVAYLAGALKQAGYTDLRFI
                     DAMTNDLSEDAVRRILAEEKPDVIGVTAITPSIYKAERLLQIAKEIHPQAVTVLGGIH
                     ATFMYNQVLTEAPWIDTIVRGEGEEIFVELIRAIDAGRWPSQRKEIKGLAYRDGDQVV
                     ATPAAPTVKNLDSISPDWSVLEWQKYIYIPMNKRVAIPNMARGCPFTCSFCSQWKFWR
                     DYRIRDPKKVVDEIETLVRDHDVGFFILADEEPTINKKKFVAFCEELISRNLGILWGI
                     NTRVTDILRDEALLPFYRKAGLIHVSLGTEAAAQLQLDRFHKETTVAQNKKAIKLLRD
                     AGIVVEAQFIVGLENETAETLEETYRMAQDWKPDLANWSMYTPWPFSDLFRELGDKVE
                     VFDYEKYNFVTPIMKPDAMDRGELLNRVMNNYRRFYMKKALFSYPFAGTGERRRYLWG
                     CLKAFLKSGFERKFYDLGRVNYWGPQSKGKVDFGFDMSRKRADTSRVEWKTTHNRKLK
                     GDDQAKIMACGGGIEQMAEADEHVPAAE"
BASE COUNT          822 a         1391 c         1518 g          965 t
ORIGIN      
        1 gtgcgggcat tcctgccgct cgcgggaaga tgccatgacg catcgacgat cgcgatgtcg
       61 ggcgaggcca gatgcttctc gagccagttg gtggaaacga tgtcctcgct catgcgaata
      121 ccactcggat tcggcggttg atcctaccct tgttctcgat ctttcccacg gcaactggac
      181 cgatgccgcc ggtggattgc aagtgggtgc cgccacaggg ctggaggtcg atcttgccgt
      241 tctcgccgat caagacgaga cggacgcggc cggtgcccat gggcggcttc acagacatgg
      301 tccggatgag atgctgattc tcttccatct cggcatcggt gatccaacga tagctcaccg
      361 ggtgatcttc ggcgatcagg cggttgatgc gttcggaaag tgcagccttg tcgggaatct
      421 cgccttccgg aatgtcgaag tcgatgcggc cgccatcctc cgtgatggcg ccaccggtca
      481 ccggaaatgg caccgaggcg cagaggagat gcatcagcgt gtgcgcccgc atgttgcgat
      541 ggcgattgtc ccaatccagc gtggccatga ccgtggcgcc cacggcgggt gttgcacaat
      601 tcggttcggg gacatgcacg acattcttcg ccgcgtcata gatggtggtg gcgattgata
      661 tttccccacc gtcccacgcg atgcgcccct tgtcccctgg ttggccgccg gcggtggcat
      721 agaaaaccgt gcggtcaagc aggatgccgc cccgctcatc gattgccgag accgtggccg
      781 tgcaggatct cagctgcgaa tcctcgcgaa acagcagtgc cgtcacgctg gaacctcttc
      841 ggtgacgtcc ggaaccctgg gcactgcttc cagccaggtt ggcacgggca ggttcttctc
      901 gcgcaggaat gtggggttga acagctttga agcatagcgc gtgccatagt cacagagaat
      961 ggtcacgatc gtatgacccg ggccgagcgc cttggccatg cggatggcac ccgcgacatt
     1021 gacgcccgtc gagcctccca tgcagaggcc ttcgtgctcg agcaggtcaa acgcgatcgg
     1081 gatggcctcg ctgtccggga tctggaaggc ctcgtccacg atggcgccct ccaggttggc
     1141 ggtgatgcgg ccctggccga ttccttccgt gatggaggag ccttcggact tgagttcgcc
     1201 gaacttgtag tagttgtaca gagcagcgcc catcgggtcg gccagggcga tcttgatcgc
     1261 cggattgcgc gatttcaggc ccatgctgac gccggctagc gttccgcccg agccgacggc
     1321 cgcgacgaag gcatcgacct tgccgtccgt ctgcgcccag atttccgggg ccgtggtctc
     1381 cacatgggcg aggcggttgg ccgtattgtc gaactgattg gcccagacgg cgcccatggc
     1441 cttggcgaga cggccggaat acttcacgta gttgttgggg ttcttgtagg gaacggcagg
     1501 aacctcgacc agctcggcac cgagctggcg caaagcgtcc ttcttctcct gcgactgggt
     1561 cacgggaatc acgatgaccg agcggaagcc catggcgttg gcgaccacgg tcaggccgat
     1621 gccggtgttt ccggccgtgc cttcgacgat cgtacccccg ggcttcaagc tgccgcgggc
     1681 aatggcgtcg cggatgatga acagcgctgc ccggtccttg accgactgac ccgggttcat
     1741 gaactcggcc ttgccgagga tggtgcagcc cgtcagttcc gaggccttgc ggagtttgat
     1801 gagcggggta ttgccgatgg cggcgatgac gtcttgtttg atatccatcc gctagacatg
     1861 ccccatgcga ggctggtccg tcaaatggac ttggttctgc tgtcccgtca ggctcctggt
     1921 caatcccagg acagaaaagg ccgggcacga agcccggcct tgaagctggc tccccgagct
     1981 ggactcgaac cagcgaccat tcgattaaca gtcgaatgct ctaccaactg agctatcggg
     2041 gaatgacttg gcgcgcgtat agcagaggcg tccggccggg aaaagagttt ttctgggcag
     2101 cggggcgggt ctggaaaagc cataatcctc caccatatct ctagaatgtc gcgcgtcaat
     2161 tttgtcggat acaagatgaa gctccccggg catcccatcc tgcggatggg catgggactg
     2221 cttctggtgt tcttcgggct tctggggttt cttcccgttc tcggcttctg gatggtgccc
     2281 ctcggcctgc tcattctgtc ggtagacctt ccgttcgtga ggcgctggac acggcttctc
     2341 aatgtcaggt tggggcactt cctgcaacgc cgctggccca gtcttgcccg caagatcggg
     2401 tacggcatgc gccgcgaccg cggttaaccg gcctcaggcc cagtcgatcc ggaatcgaca
     2461 ggccttagcg cccatgccgg cgcattccgt ctcggtgacg tgcgcatccg cagaaacgag
     2521 ttccctgaag aggcgctcga aggttgcggc atagtaggtg cagcagggcg actgggaggt
     2581 tgcattccgg cagagcggac agtcttcgat cgagaaatgc accggcttga agccgatata
     2641 ggtgaacctg ccggatccgg tgaatgtcca ggcgtggcgc gagatcgcct gaagcaggat
     2701 acgcccagca aggggcgccg gcagccgccg caggaggtgt tgcaccgggg cgggaatgcg
     2761 gttggcgagc agatagttgg ccgtatggtg gcctgcgacc caggagacgc tggccgagtg
     2821 tctctcgccg agacctgcgc gcagccggtg atgcagttcc acgacatccg actcggcaat
     2881 catggtttcc ggcgggcgtt ccagataatg cgcgatgccc gcttcgccga agatccgggt
     2941 cgaagccgat ctgccctcga cagcgtcaag cgcctttgcc acttgaatca ccgcattggg
     3001 accgatcagc gctgcaacag cctgtgacat gccggactat tccgccgcag ggacgtgctc
     3061 gtcggcctct gccatctgtt cgatgccgcc gccacaggcc atgatcttgg cctgatcgtc
     3121 gcccttgagc ttgcggttgt gggttgtctt ccactcgacg cgtgatgtgt cggcgcgctt
     3181 gcgcgacatg tcgaagccga agtcgacctt gcccttggac tgcggacccc agtagttgac
     3241 ccggcccagg tcatagaact tccgctcgaa gccggacttg aggaaggcct tgaggcaccc
     3301 ccaaagatag cgccgccgct cgccggtgcc ggcaaaggga taggagaaga gcgccttctt
     3361 catgtagaaa cggcggtagt tgttcatgac acggttcagc agttcaccgc gatccatggc
     3421 gtcgggcttc atgatcgggg tcacgaaatt gtacttctcg tagtcgaaga cctcgacctt
     3481 gtcgcccagt tcgcggaaga gatcggagaa gggccatggc gtgtacatcg accagttggc
     3541 gaggtcgggc ttccagtcct gcgccatgcg gtaggtttct tcgagcgttt ccgccgtctc
     3601 gttctcgagg ccgacgatga actgcgcttc caccacgata ccggcatcgc gcagcagctt
     3661 gatggccttc ttgttctggg cgacggtggt ttccttgtgg aaccggtcga gctgaagctg
     3721 ggccgcagcc tcggtgccga gcgacacatg gatcaggcct gccttgcggt agaagggcag
     3781 cagcgcctcg tcgcgaagga tatcggttac ccgcgtgtta atgccccaga ggatgccaag
     3841 gttgcggctg atcaactcct cgcagaaggc cacgaatttc ttcttgttga tggtcggttc
     3901 ttcgtcggcg aggatgaaga agccgacgtc gtggtcgcgc acaagggtct cgatctcatc
     3961 caccaccttc ttcggatcgc gaatgcggta gtcgcgccag aacttccatt gcgagcagaa
     4021 gctgcaggtg aaggggcagc cgcgtgccat gttgggaatg gcgacgcgct tgttcatggg
     4081 aatgtagatg tatttctgcc actcgagcac gctccagtct ggcgagatgg agtcgaggtt
     4141 cttgaccgtg ggtgccgcag gtgtggccac gacctgatcg ccgtcgcgat aggcgagccc
     4201 cttgatctcc ttgcgctggg acggccagcg gcccgcgtcg atggcgcgga tcagctcgac
     4261 gaagatctct tcgccctcgc cccgcacgat cgtgtcgatc cagggcgcct cggtgaggac
     4321 ctgattgtac atgaaggtgg catgtatccc gccaaggacc gtcacggcct gcggatggat
     4381 ctcctttgcg atctgcagca ggcgctcggc cttgtagatc gaaggcgtga tcgcggtgac
     4441 accgatcacg tcgggctttt cctcggcaag gatgcggcgg acggcatcct cggaaaggtc
     4501 gtttgtcatg gcatcgatga accgcaggtc tgtgtagccc gcctgcttca gggcgccagc
     4561 aagataggcc acccaggcgg gcggccagtt gccggcgatc tcggcgccgc cggagtggtg
     4621 gttcgggtgg atcagcacga ctttcatggg cgggccctca ttgcgtgaag tgtctcgaca
     4681 atagtacagt tcgatc
//
LOCUS       DBNGVR010000429         3367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_353644, whole genome shotgun sequence.
ACCESSION   DBNGVR010000429 DBNGVR010000000
VERSION     DBNGVR010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_353644"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="deoC"
                     /locus_tag="AC16A0_09110"
     CDS             complement(<1..719)
                     /gene="deoC"
                     /locus_tag="AC16A0_09110"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR3105534.1"
                     /translation="MAQTNIIPLPHHVRNAGFAFDRDWIDGLRVNLSAAERRVATLPG
                     RRSVKKDAQAAWLLKAVTCIDLTTLSGDDTAGRVKRLCAKAIHPVRADILEGLGMGDR
                     GLHTGAVCVYHRFVATAAEALTGTGIPVAAVSTGFPAGLVPHDVKLREIEASVRDGAA
                     EIDIVITREHVLTGNWKALYDEMRDFREACGSAHVKAILATGDLKTLRNVAKASMICM
                     MAGADFIKTSTGKEGVNATLLV"
     gene            complement(757..1197)
                     /locus_tag="AC16A0_09115"
     CDS             complement(757..1197)
                     /locus_tag="AC16A0_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3105535.1"
                     /translation="MLPPTQTLYFEDLSIGMTEVYVKEVKSSDVVGFAEISGDRNPIH
                     LSEHFAARTPFGGRIAHGLYTASLISAVIGTRRPGPGAVYMSQTLNFKAPVRIGDMVE
                     ASVEVVELVEKGRRVKLACRCMVGETLVLEGEALVKVPGKPEGY"
     gene            complement(1210..1428)
                     /locus_tag="AC16A0_09120"
     CDS             complement(1210..1428)
                     /locus_tag="AC16A0_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105536.1"
                     /translation="MQNVSTTELAITVSALLAGFGLVAGMIVLERRPRTSLNPRLIPT
                     TPVMLLGALVAILAIVHLVNLYGVHTGR"
     gene            1542..1667
                     /gene="ykgO"
                     /locus_tag="AC16A0_09125"
     CDS             1542..1667
                     /gene="ykgO"
                     /locus_tag="AC16A0_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HIR3105537.1"
                     /translation="MKVRNSLKSLIKRHRDNRLVRRKGRVYIINKTNPRFKARQG"
     gene            1761..2348
                     /locus_tag="AC16A0_09130"
     CDS             1761..2348
                     /locus_tag="AC16A0_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3105538.1"
                     /translation="MRHASCLLLACLMFATPASAEEALPAPPEVAKSPAEMRSESLDR
                     LIASLHTAGAEANAKTLEKKIWDLWMASDSPTAEVLLRQASKATDEGAPEEALKILDK
                     LVERYPDFAEAWNRRATLYYLMRRYDAALADIEKVLELEPRHFGALAGRGMIYQKKKN
                     YAAAIDAYREALTMNPLMEGPKEALEELERLEQAI"
     gene            complement(2370..>3367)
                     /gene="pyk"
                     /locus_tag="AC16A0_09135"
     CDS             complement(2370..>3367)
                     /gene="pyk"
                     /locus_tag="AC16A0_09135"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3105539.1"
                     /translation="IFGGTLSDRKGVNLPDTVIPIPALTAKDRSDLEAAAEAGVDWIA
                     LSFVQRPDDVAEARKLIAGRAGVMAKIEKPTALHCLDEILEIADGLMVARGDLGVELP
                     LEAVPGKQKQITRAARRMGKPVIVATQMLESMIVEPVPTRAEVSDVATAVYEGADAIM
                     LSAESATGAWPDKAVATMDRIAREVERDSLYRGIITAQRYEPQATSADAISGAARTIS
                     ETLKLAAIVCFTGSGSTGVRMSRERPETPVIALTPIPATARRLALVWGLHCVLTEDAK
                     DLDDMVSRAARIAVDEGFAEPGQRIIITAGVPLGTPGATNMLRIAYVGRNGATGN"
BASE COUNT          606 a         1050 c         1098 g          613 t
ORIGIN      
        1 accagcagcg tggcattcac gccctccttg ccggtggagg tcttgatgaa gtcggcgccg
       61 gccatcatgc agatcatcga ggccttcgcg acattgcgca gcgtcttgag gtcgccggtg
      121 gcgaggattg ccttcacatg cgcagagccg caggcctcgc ggaagtcgcg catctcgtcg
      181 tagagtgcct tccagttgcc ggtgaggaca tgttcgcggg taatgacgat gtcgatctcg
      241 gccgcaccgt ctctaaccga ggcttcgatc tcgcgcagct tcacgtcatg cgggacaagg
      301 cccgcgggga aaccggtgga gacggcggca accggaatgc cggttccggt caacgcctcc
      361 gccgcggtgg cgacgaagcg atgatagacg cagacagcgc cggtatggag tccgcgatcg
      421 cccatgccca ggccttcgag aatatccgcc cgcacgggat gaatggcctt ggcacagagc
      481 cgcttgacgc gacccgccgt atcgtcgccg ctcagcgtgg tgaggtcgat gcaggtcacg
      541 gccttgagca gccaggcggc ctgggcatcc ttcttcaccg agcggcggcc cggaagcgtt
      601 gcgacgcggc gctctgcagc ggagagattg acgcgcaggc catcgatcca gtcacggtcg
      661 aaggcaaagc ccgcgttgcg cacgtgatgg ggcaggggaa tgatgttggt ctgagccatg
      721 acaggaacta gaaacgtcgc agaagtttag tcaagatcaa tagccttccg gcttgcccgg
      781 cactttcaca agtgcctcgc cctcgaggac cagtgtctcg cccaccatgc agcggcaggc
      841 cagtttcacc cgtcggccct tctcgaccag ttcgaccact tcgaccgaag cttccaccat
      901 gtcgccaatc cggacaggcg ccttgaagtt gagcgtctgc gacatgtaga ccgcgccggg
      961 gccgggccgg cgcgtgccaa tgacggcaga gatcaggctt gccgtgtaga ggccatgggc
     1021 gatacgcccg ccgaaggggg ttcgggccgc gaagtgttcg gacagatgga tcgggttccg
     1081 gtctccgctg atctcggcga agccgaccac atcggacgac ttcacctcct tcacatagac
     1141 ttccgtcatg ccgatggaga ggtcttcgaa gtagagtgtc tgggtcgggg gcagcatggt
     1201 gttcatcgac tatctcccgg tgtgaacgcc atagagattg accagatgca cgatggccag
     1261 gatcgcgacc agtgcgccca gaagcatgac gggggttgtg gggatgagcc gcgggttcag
     1321 gcttgtccgg gggcggcgtt cgagaacgat cattccggcc acgaggccaa atccggcaag
     1381 aagggcgctt acggtgatcg cgagttcggt ggtggagacg ttttgcatga tggagtaggg
     1441 tccggttgac aggctcggcc cgcccctcta tagacctgcc tcaattctcg caggcgttgc
     1501 ggacctctgg ttccatgtgc ctgaaaaatc aggtgaagtc catgaaagtt cgtaattcct
     1561 tgaagtcgct gatcaagcgg caccgcgata accggctggt ccgccggaag ggccgggttt
     1621 acatcatcaa caagaccaat ccgcgcttca aggcgcggca gggctgaacc ttatctccct
     1681 gacggatttg tgaacgccgc gggcagccac tgcccgcggc gctcattttg ttgccgcagg
     1741 ctttggtgta ttctctcacc atgcgccacg cctcatgcct gctcttggcc tgcctgatgt
     1801 tcgccacccc ggcaagcgcc gaggaggcgc ttcctgcgcc tccggaggtc gccaagtccc
     1861 cggcggagat gcgatccgag tcgcttgatc ggctcattgc cagcctgcac acggctggcg
     1921 cagaggcaaa tgccaagacc ctcgagaaga agatctggga cctctggatg gcctcggatt
     1981 cgccaaccgc cgaggtcctg ctgcgccagg ccagcaaggc gaccgacgaa ggcgctccgg
     2041 aagaagccct caagatcctg gacaagctgg ttgagcgcta tcccgatttt gccgaagcct
     2101 ggaatcgccg tgcgaccctt tactatctga tgcggcgcta cgatgccgct cttgccgata
     2161 tcgagaaggt gttggagctg gaaccgcgcc acttcggggc ccttgccggc cgcgggatga
     2221 tctaccagaa gaagaagaac tacgccgccg ccatcgacgc ctatcgcgag gccttgacca
     2281 tgaacccgct catggaaggc ccgaaggaag ccctcgagga actggaacgg ctggagcagg
     2341 ccatctgacc gccgaggtcg gtctggccgt cagtttcctg tcgccccgtt cctgcccaca
     2401 taggcaatgc gcagcatgtt ggttgcgcca ggcgttccca gcgggacgcc ggccgtgatg
     2461 atgatgcgct ggcctggttc ggcaaagcct tcatcgacgg caatgcgggc cgcgcgcgag
     2521 accatgtcat cgaggtcctt ggcatcttcg gtcagcacgc agtgaagacc ccagaccagc
     2581 gcgaggcggc gagccgttgc ggggatcggc gtcagcgcaa tcaccggcgt ttcgggtctt
     2641 tcgcgcgaca tgcgcacgcc ggtcgatccg gagccggtga aacagacgat cgccgccagc
     2701 ttgagagttt ccgagatggt gcgggccgcg cccgaaatgg cgtcagcact tgtcgcctgc
     2761 ggctcatagc gctgggcggt gatgatgccg cgatagaggc tgtcgcgctc cacttcgcgc
     2821 gcaatccggt ccatggtcgc cacggccttg tccggccacg cgcccgtggc gctttccgcc
     2881 gacagcatga tggcatcggc gccttcgtag acggcggtgg cgacgtcgga gacttctgcg
     2941 cgggtcggca ccggctccac gatcatcgat tccagcatct gcgtggcgac gatcaccggc
     3001 ttgcccatgc ggcgcgcggc gcgcgtgatc tgcttctgct tgccgggcac ggcctcgagc
     3061 ggcagttcga cgccaaggtc gccacgggca accatgaggc catcggcaat ttcgaggatt
     3121 tcgtcgaggc aatgcagggc cgtgggcttc tcgatcttgg ccatgacgcc ggcgcggccg
     3181 gcaatcagct tgcgcgcctc ggccacgtcg tccgggcgct gcacgaatga cagggcgatc
     3241 cagtcgactc cggcttcggc cgccgcttca agatcggagc ggtccttcgc ggtcagcgcc
     3301 ggaatcggaa tgaccgtatc ggggagattg acgcccttgc gatcggacag cgtgccgccg
     3361 aagatca
//
LOCUS       DBNGVR010000430         3571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5264153, whole genome shotgun sequence.
ACCESSION   DBNGVR010000430 DBNGVR010000000
VERSION     DBNGVR010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5264153"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="AC16A0_09140"
     CDS             complement(<1..412)
                     /locus_tag="AC16A0_09140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIR3105540.1"
                     /translation="MGPETSCVKGEGFALVARTATPYLSAVKAQQSFFQRLIPLRFRK
                     SVPVVTHVRLQGAIGVGTPMRPPLTFKDVEPQLERAFRKGVAAVAITVNSPGGSPVQS
                     ALIHDRIRALAVEFKLPVYVFCEDVAASGGYWLAC"
     gene            490..1482
                     /locus_tag="AC16A0_09145"
     CDS             490..1482
                     /locus_tag="AC16A0_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3105541.1"
                     /translation="MELVSGLLLATWMTKPVWMWLAFLAIVIVLLVLDLGVLHKENRE
                     IGVRESLLLSAFYIALGLGFGGWVWFQLGASAGIEYLTGFAVEKALAMDNVFVIAMIF
                     SFFAVPRKYQHRVLFWGILGVILLRAIMIGLGAAIVSEFSWVLYIFALFLIATGIKMI
                     VFADKDYDVANNPVLRFVRRHFNVTDEHHAERFFVKKPDPKSGKLVWFMTPLFLALVL
                     IEVADVIFAVDSVPAIFAITTDPFIVYTSNIFAILGLRALYFALAAMVHRFHYLKLAL
                     AIVLIFIGSKIFAADLLGIDKIPPALSLAITFGILASGVLYSLWKTRSAETAVR"
     gene            complement(1479..2294)
                     /locus_tag="AC16A0_09150"
     CDS             complement(1479..2294)
                     /locus_tag="AC16A0_09150"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF017026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - obsolete tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR3105542.1"
                     /translation="MSATALSASPPPPPSGGTGVPSSLTEDGFLGGRLQILQPAKGFR
                     AGIDSVFLAATIPCNAGESVFEAGVGTGVAALCTLARTPGIHLTGIEVAARYALLCEE
                     NAKRNGFGGNVRVIHADIKEALRKDLASMPAHGTFSHAFANPPYFDDSKSTPSPVLLK
                     AQAHAFGPDDLELWIRVMHTMVTMRGTVTLVNRADALGKILQVMEERFGDIRIAPLYA
                     REGTSASRVIVQGVKGSKAPMQLLPGLVLHDSSSQFTPEADAILREGMAWRLR"
     gene            complement(2311..2526)
                     /locus_tag="AC16A0_09155"
     CDS             complement(2311..2526)
                     /locus_tag="AC16A0_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3105543.1"
                     /translation="MKELLRSNDLVYLSFVGHVLDEAGIDYLLLDGYTSAVEGSIGAI
                     PRRILVEESLLDRARRELGNAALTIPG"
     gene            2678..>3571
                     /locus_tag="AC16A0_09160"
     CDS             2678..>3571
                     /locus_tag="AC16A0_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3105544.1"
                     /translation="MPHDTPLIAILAVGLGLAFVMGLVAQRLRLPPIIGYLVTGIIIG
                     PFTPGFVADQALAAELAEIGVILLMFGVGLHFSLKDLLSVRAIAIPGAIGQIAAATLL
                     GMVLAWAMGWSVGAGLVFGLSLSVASTVVLLRALQERRLVETERGRIAVGWLIVEDLV
                     MVLTLVLLPPLAGLLGGIETRGTGDFGLASRLGIGTVWTTLGVTAVKVGIFVALMLVV
                     GRRVIPWVLHYVAHTGSRELFRLAVLAIALGVAYGSASLFGVSFALGAFFAGMVLAES
                     QLSTQAAKETLPLRDAFAVLFF"
BASE COUNT          550 a         1171 c         1152 g          698 t
ORIGIN      
        1 cgcaggccag ccagtagccg cccgaggcgg ccacatcctc gcagaagaca tagaccggca
       61 gcttgaactc caccgccagc gcgcggatgc ggtcatggat cagcgccgac tgcaccggcg
      121 agccgccggg cgaattgacg gtgatggcta ccgcagcgac ccccttgcgg aacgcgcgct
      181 ccagctgcgg ctccacatcc ttgaaggtga gcggcggccg catcggcgtg cccacgccaa
      241 tggcgccctg aagccgcaca tgggtgacca cgggaaccga cttgcggaag cgaagcggaa
      301 tgaggcgctg gaagaaggac tgttgggcct ttaccgcact tagataagga gtggcagtgc
      361 gcgcgacaag cgcgaagcct tcccccttga cgcaggatgt ctctggtccc atatccccgc
      421 cgttccgggc ccctctggcg ctctcgcggg cgcgatgtcg gacacatcga tgcattaaag
      481 agggtttcaa tggaacttgt gtccggactt ctgctcgcca cctggatgac caagccggtc
      541 tggatgtggc tggcctttct cgccatcgtc atcgtgcttc tggttctcga tctcggcgtc
      601 ctgcacaagg agaatcgcga gatcggcgtg cgcgaaagcc tcctgctctc ggccttttac
      661 attgcccttg gcctgggttt cggcggctgg gtctggttcc agctgggcgc cagcgctggt
      721 atcgagtatc tcaccggctt cgccgtggag aaggcgcttg cgatggacaa tgtcttcgtc
      781 atcgccatga tcttctcgtt cttcgcggtg ccccgcaagt accagcaccg cgtgctcttc
      841 tggggcatcc tcggggtgat cctgctgcgc gccatcatga tcggcctcgg cgccgcgatc
      901 gtcagcgaat tctcatgggt gctctacatc ttcgccctgt tcctgatcgc caccggcatc
      961 aagatgatcg tctttgccga caaggactac gatgtcgcga acaatcccgt gctgcgtttc
     1021 gtgcggcggc acttcaacgt taccgacgag catcacgcgg aacgcttctt cgtgaagaag
     1081 ccggacccga agagcggcaa gctcgtgtgg ttcatgacgc ccctcttcct ggcgctggtg
     1141 ctgatcgagg tggcggacgt gatcttcgcg gtggattccg tgccggccat cttcgccatc
     1201 accaccgacc ccttcatcgt ctacacctcg aacatcttcg ccattctcgg cctgcgcgcg
     1261 ctctacttcg cgctggcggc gatggtgcat cgcttccact atctgaagct ggcccttgcc
     1321 atcgtgctga tcttcatcgg ctcgaagatc tttgccgccg acctgctggg catcgacaag
     1381 atcccgccgg cgctgtcgct ggcgatcacc ttcggcatcc tggcctcggg cgtgctctac
     1441 agcttgtgga agacgcggag tgccgagacg gcggtgcgct aacgcaggcg ccaggccatg
     1501 ccttcgcgca ggatggcgtc ggcttcgggg gtgaactgcg agcttgaatc atgaagcacc
     1561 agaccgggca gcagctgcat cggcgccttt gagcccttga cgccctgcac gatcacgcgc
     1621 gaggccgaag tgccctcgcg cgcatagagc ggcgcgatgc ggatgtcgcc gaagcgctct
     1681 tccatcacct gcaggatctt gcccagcgca tcggcgcggt tcaccagcgt cacggtgccg
     1741 cgcatggtca ccatggtgtg catgacgcga atccacagct caagatcgtc ggggccgaag
     1801 gcatgggcct gcgccttgag cagcaccggc gagggcgtcg acttgctgtc gtcgaaatag
     1861 ggcggattgg cgaaggcatg gctgaaggtg ccatgggccg gcatggaagc caggtccttg
     1921 cgcagcgcct ccttgatgtc ggcatggatg acgcggacat tgccgccgaa gccgttgcgc
     1981 ttggcattct cttcgcagag cagggcatag cgtgccgcga cttcgattcc ggtcaggtgg
     2041 atgcccggcg tgcgggccag ggtgcagagt gcagccacgc cggtgccgac gccggcctcg
     2101 aagaccgatt cgcccgcatt gcaggggatg gtggccgcca ggaaaaccga gtcgattccg
     2161 gcacggaagc ccttcgcggg ctgcaggatc tgcagccgtc cgccgagaaa tccgtcctcg
     2221 gtgagcgacg acggcacgcc cgtaccgccc gaagggggcg gcggcgggga ggccgaaagg
     2281 gctgtcgcgc tcatttgcat tcaagccaat ctatcccggg atggttagcg ccgcgttacc
     2341 aagctcgcgg cgggcgcgat ccagaaggga ctcttcaacc agtatccgcc ggggaatggc
     2401 cccgatcgag ccttcgacgg cgctggtata gccatcgagc agcaggtagt cgatcccggc
     2461 ctcgtccagc acatggccga cgaaggagag gtagaccaga tcgttgctgc gcagcagttc
     2521 cttcatgccc gcatcagact ggccgcaaac ccgcaaaagg taaacagggc tcgcagccga
     2581 tccaagagat cttggatttt ctctcgtatt tccgagggtt tgccctagta ttgcccagac
     2641 gatccggtag ttcggccggt tatcgagagg agcccctgtg ccgcacgaca cgccgctcat
     2701 cgccatcctg gccgtaggcc tgggcctcgc cttcgtgatg ggcctcgtcg cgcagcggct
     2761 gcggctgccg ccgatcatcg gctatctggt gaccggcatc atcattggcc ccttcacgcc
     2821 aggtttcgtg gccgaccagg cgctggccgc cgagctggcc gagatcggcg tcatcctcct
     2881 gatgttcggc gtcggcctgc atttttccct gaaggacctg ctgtcggtgc gggccatcgc
     2941 gattcccggc gccatcggcc agatcgcggc cgccaccctg ctcggcatgg tcctggcctg
     3001 ggccatgggc tggtcggtcg gcgcgggcct cgtcttcggc ctgtcgctgt cggtcgccag
     3061 caccgtcgtc ctgctgcgcg cgctgcagga acgccgcctg gtcgagaccg aacgcggccg
     3121 catcgcggtg ggctggctca tcgtcgagga tctggtgatg gtcctgacgc tcgtgctgct
     3181 gccgccgctc gcgggcctcc tgggtggcat cgagacgcgc ggcacgggcg acttcggcct
     3241 ggcctcgcgg ctgggcatcg gcacggtgtg gaccacgctc ggcgtgaccg ccgtgaaggt
     3301 gggcatcttc gtcgccctga tgctggtcgt cgggcgccgg gtcattccct gggtcctgca
     3361 ctatgtcgcc cataccggct cgcgcgaatt gttccggctc gcggttctgg ccatcgcgct
     3421 gggcgtggcc tatggctcgg ccagcctctt cggcgtctcc ttcgcgcttg gcgccttctt
     3481 cgccggcatg gttttggcgg aatcgcagct cagcacccag gccgccaagg aaaccttgcc
     3541 gctgcgcgac gccttcgcgg tgctcttttt c
//
LOCUS       DBNGVR010000431         4701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1926814, whole genome shotgun sequence.
ACCESSION   DBNGVR010000431 DBNGVR010000000
VERSION     DBNGVR010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1926814"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1109
                     /locus_tag="AC16A0_09165"
     CDS             81..1109
                     /locus_tag="AC16A0_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HIR3105545.1"
                     /translation="MTDGQRQPRAFVINPPDPHEIKTETRRKPRAITGIRFEPEGGGD
                     QPVVAPAPLPVSRQFRWGMLLGGALFSLIVMWAGLSISQLIEDFFSRSPALGWLASGL
                     AALAALAAGAIILREVLALIRLRKIESIQADAAHTLNADDPASGKRVIAELRSLYGNR
                     PEQQWGLTALAEHDSDIMDPRDRLRLAERYLIIPRDEDAHRIIARAARRVTLLTTVTP
                     AAALDILFVAAQNLRMMRELASLYGGRPSTLATLRLARMVVSHLAVTGGLALSDNLLQ
                     HVVGKGLLGRLSARFGEGAVNGILTARIGLAARDVCRPVPQETTAKETLGSLLRELVA
                     TDGAKSPE"
     gene            1106..2071
                     /locus_tag="AC16A0_09170"
     CDS             1106..2071
                     /locus_tag="AC16A0_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIR3105546.1"
                     /translation="MNARANLDPDVQRRHNLRNLLQTVILIVGTAALASAIAWVTFGP
                     SGLIWAAAFGAIGIWFLGRASPRMVLRLYKARELADHEVPELHDLMRTLAKRADLQAV
                     PRLYYVPSQMLNAFAVGNSADSAVAVTDGLLRIMNLRQLAGIMAHEVSHIRNGDLRVM
                     GLADVLNRITGFMSTLGLLGIPLLLGTGWDIPIVGLLLLIFAPTIGGLLQLALSRARE
                     YDADLDGATLTGDPEGLASALALLERKQGGAWEGLILPGSRLPEPSILRTHPKTADRI
                     ERLLALRHEATMAIPLAETRTATASSLVPPVRPPRIHWQRLGIWY"
     gene            2086..2400
                     /locus_tag="AC16A0_09175"
     CDS             2086..2400
                     /locus_tag="AC16A0_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105547.1"
                     /translation="MLALNSGLAEASNRSTRIAMAVALLDVAEEHCGSSIEVDPEMRK
                     TLFLHFHEYDIAGLASVISRELNAFYEDFSAVMKKDRAAFCRSSPNYAASAGYPLIQM
                     AE"
     gene            2405..3580
                     /locus_tag="AC16A0_09180"
     CDS             2405..3580
                     /locus_tag="AC16A0_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3105548.1"
                     /translation="MFLRLFNELRQAGVPVSLTEYLLLVESLDRGLAGHSTEEFYYLS
                     RAALVKDERNLDRFDRVFASVFRGVESAGDDLMAEVPDAWLRKLTEKFLTDEEKRQVE
                     ALGGLDKILEELRKRLEEQKGRHQGGKKWIGTAGTSPYGAYGYNPAGVRIGQDSNRNN
                     RAVKVWDRRDFQDLDDKVELGTRNIKLALRRLRRFARTGAVEELDLDGTIRATARQGW
                     LDVKLVPEKRNAVKVLLFFDVGGSMDPHVRICEELFSAARSAFKHMEYFYFHNCIYES
                     LWKDNRRRNTERTATLDVLHTYPNDYKVIIVGDAAMSPFELTHAGGSVEHWNEEPGIA
                     WLQRIAATFPRTVWINPAPERYWSYSPSRELIARIFEGRMFPLTLDGLDRAMRDLAR"
     gene            3667..4227
                     /locus_tag="AC16A0_09185"
     CDS             3667..4227
                     /locus_tag="AC16A0_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HIR3105549.1"
                     /translation="MQKKTKIALAAGLAGILGLGTLAGVAVADGGWGGGHGMGGGHGK
                     GRMAQQMMERYDANKDGKVSQEEIDTNRTQWHGEFDADKNATLSIAEFEKLWLKARFE
                     QMVREFQRFDRDGNGQLTLEEYKGPLADLVAERDRNKDGFLSREDRPQRGEGHGWRHG
                     KRGDRMQQDGSDGDGPPPLEDSDDAQ"
     gene            4398..>4701
                     /locus_tag="AC16A0_09190"
     CDS             4398..>4701
                     /locus_tag="AC16A0_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105550.1"
                     /translation="MAIAEPNKPPMPFAIAPTQAVPQAGTHSLPHDAALAHVHNLADF
                     AKLETDWRELERQSPATSLFQSFDWLQSWAAVYLTEGNSTQPFIVTGTLGGKLVFAW"
BASE COUNT          876 a         1531 c         1458 g          836 t
ORIGIN      
        1 cctccggcac caaaggacgg gcgctttcct cacatccggc tcgaccggac cgcagagttc
       61 ctgatcggag accgcctcgc atgaccgacg gccagcgaca accccgggcc ttcgtcatca
      121 atccgcccga cccgcacgag atcaagaccg agacgcggcg gaagccacgc gccatcacgg
      181 ggatccgttt tgagcccgag ggcgggggcg atcaacctgt cgtggcgcct gcccccctgc
      241 ccgtctcgcg ccagtttcgc tgggggatgc tgctgggtgg agcgctcttt tcgctcatcg
      301 tcatgtgggc ggggctgtcc atctcgcaac tgatcgagga cttcttcagc cgctcgccgg
      361 cgctgggctg gctcgcttcg ggactggccg cactggccgc tctcgcggcg ggagccatca
      421 ttctgcgcga ggtacttgcc ctcatccgtc tgcgcaagat tgaatcgatc caggccgatg
      481 ccgcccacac gctcaatgcg gatgacccgg caagcggcaa gcgggtgatt gcggagctgc
      541 gcagcctcta tggcaaccga cccgaacagc agtggggact caccgctctc gccgaacacg
      601 acagcgacat catggatccc cgtgaccgcc tgcgtctggc cgagcgctac ctgatcatcc
      661 cgcgggatga agacgcccac cgcatcatcg cgcgcgcggc gcggcgcgta acgctgctga
      721 ccacggtcac gcctgccgca gcgctcgaca ttctcttcgt cgccgcgcag aacctgcgca
      781 tgatgcgcga gctggcaagc ctctatggcg ggcgcccgtc gacgctggcc acgttgcgcc
      841 tggctcgcat ggtggtcagc catctggcgg tcaccggcgg attggcgctg tcagacaacc
      901 tcttgcagca cgtggtgggt aagggactgc tgggccggct ttcggcccgt tttggcgaag
      961 gtgcggtcaa tggtatcctg accgcgcgca tcggacttgc cgcccgcgat gtgtgccggc
     1021 ccgttccgca ggaaacaacg gccaaggaaa cgctcggcag cctcctccgg gagctggtcg
     1081 caacagacgg agccaagtcg cccgaatgaa tgccagagcc aatctcgatc ccgacgttca
     1141 gcgccgccac aatctgcgca acctgctgca gaccgtcatt ctcatcgtcg gtaccgcagc
     1201 gctcgcctcg gccatcgcct gggttacctt cggcccgagc ggcctgatct gggccgcagc
     1261 cttcggcgcc atcggaatct ggtttctcgg ccgcgcctcg ccgcggatgg tgctcagact
     1321 ctacaaagcg cgtgaacttg ccgaccatga agtgcccgag ctgcacgatc tcatgcgaac
     1381 gctggcgaag cgcgcagatc tccaggcggt gcctcgtctc tactatgttc caagccagat
     1441 gctgaacgcc tttgccgtgg gaaacagcgc cgactcggcc gtggccgtca ccgatggcct
     1501 cctgcgcatc atgaaccttc ggcaattggc cggcatcatg gcgcatgagg tctcgcacat
     1561 ccgcaacggc gaccttcggg tcatgggact ggccgatgtg ctgaatcgca tcaccggctt
     1621 catgtcgacg ctgggcctgc tgggcatccc cttgctgctc ggcacggggt gggacattcc
     1681 catcgtggga ctgctgctgc tgatcttcgc gccgacgatc ggcggcctgc tgcaactcgc
     1741 cctgtcacgc gcgcgcgagt atgacgccga cctggatggt gcaacgctga ctggagatcc
     1801 ggaaggtctt gcctcggccc tcgccttgct cgagcgcaag cagggcggag catgggaggg
     1861 cctcattctg ccgggctcgc gcctcccgga gccatccatc ctccgcaccc atcccaagac
     1921 ggccgaccgt atcgagcggc tcctggccct gcgccacgag gcgacgatgg ccattccact
     1981 ggcggagacc cgcacggcca cggcctcttc cctggtgccg ccggtccgac caccccgcat
     2041 tcattggcaa aggcttggca tatggtattg agactggcgc tgcttatgct ggccctgaat
     2101 tccgggctcg ccgaggcctc gaaccgcagc acccggatcg ccatggcagt cgccttgctc
     2161 gatgtcgccg aagagcattg cggttcaagc atcgaggtcg atccggagat gcgaaagact
     2221 ctcttcctgc attttcacga gtacgacatc gcaggcctcg catcggtgat ttcgcgggag
     2281 ctcaatgcct tctacgagga cttttccgcc gtgatgaaga aagaccgcgc tgccttttgc
     2341 cgatcatcgc caaactacgc ggcctccgcc ggttatccac tgatccagat ggcggaataa
     2401 ccttgtgttc ttgcgccttt tcaatgaatt gcggcaggct ggcgttccgg tatcgttgac
     2461 cgagtacctg ctcctggtcg aaagcctcga ccgcggtctc gccggccaca gcacggaaga
     2521 attctattat ctttcccgcg cggcactggt gaaggacgag cgcaatctcg accgcttcga
     2581 tcgcgtcttc gcatctgtct tcaggggtgt tgagtccgcc ggcgacgacc tcatggccga
     2641 ggtcccggac gcgtggctgc gcaaactcac cgagaagttc ctgaccgacg aggagaagcg
     2701 ccaggtcgaa gccctcggcg gactggacaa gattctcgaa gaactgcgca agcggctgga
     2761 ggagcagaag ggccggcacc agggtggcaa gaaatggatc ggcacggcgg gaacctcgcc
     2821 atacggcgcc tacggttaca atccggccgg agtccgcatc ggtcaggaca gcaaccgcaa
     2881 caaccgcgcc gtgaaggtct gggaccggcg cgacttccag gatctcgacg ataaggttga
     2941 actcggaacc cggaacatca agctggcgtt gcgccgcctg cgccgcttcg cccgcacggg
     3001 cgcggtggag gaactcgatc tcgacggcac cattcgcgca actgcacgcc agggttggct
     3061 ggacgtcaag ctcgtgcccg agaagcgaaa cgccgtgaag gtacttctct tctttgatgt
     3121 cggcggttca atggacccgc atgtgaggat ctgcgaggaa ctcttctcgg cggcgagaag
     3181 cgccttcaag cacatggaat atttctactt tcacaactgc atctacgaaa gcctgtggaa
     3241 ggacaatcgg cgccgcaaca cagagcggac cgccacgctc gacgtgctgc atacctatcc
     3301 gaatgattac aaggtgatca tcgttggcga tgccgccatg agccccttcg aactcaccca
     3361 tgcgggaggt tcggtggagc attggaacga agagcccggc atcgcctggc tgcagcgcat
     3421 cgctgcgacc tttcccagga cggtctggat caatccggct cccgaaagat actggagcta
     3481 cagcccttcg cgcgaactca tcgcgcgtat ctttgagggc cgcatgttcc ccctgacgct
     3541 ggacggcctg gatcgtgcga tgcgagacct ggcgcgttga gattacgaag agtttactcg
     3601 caggacagac ggattgccgt gtttgacccg tcttatggtc atctggttca ccaaaaggag
     3661 tagatgatgc agaagaaaac caagatcgcg ctggccgctg gccttgccgg aatcctgggc
     3721 ctcggcacgc tggccggcgt cgcggtcgct gacggcggct ggggcggcgg tcacggcatg
     3781 ggcggcggac acggcaaggg ccgcatggcc cagcagatga tggagcgtta cgacgccaac
     3841 aaggacggca aggtctcgca ggaggagatc gacaccaacc gcacccagtg gcacggcgaa
     3901 ttcgatgccg acaagaacgc cacactgtcg atcgctgagt tcgagaaact ctggctcaag
     3961 gcccgcttcg agcagatggt acgcgagttc cagcgctttg atcgcgatgg caacggccag
     4021 ctgacgcttg aggagtacaa ggggccgctc gccgacctcg tcgccgagcg cgaccgcaac
     4081 aaggacggct tcctcagccg tgaagatcgc ccgcagcggg gcgagggcca tggatggcgg
     4141 catggcaagc gtggagaccg catgcagcag gatggcagcg acggcgacgg accgccgccg
     4201 ctcgaagatt cagacgacgc gcaataagcc gcaactgaag gggccggaca gcgtgagggt
     4261 ccggcccctc gacctttcgc cgacatccga gcccgacaca gagccttaac cgttcgttca
     4321 ccatggtttg gcccgtcaat gagttggcag gatattggcg gctatggtcg gcttcacgaa
     4381 gtcgtgagga tcgctccatg gccattgccg agccgaacaa gccgcctatg ccctttgcga
     4441 tcgcaccaac gcaagctgtg ccccaggcag gaactcacag tcttccgcat gacgctgccc
     4501 tcgcgcatgt gcataacctc gccgatttcg caaagcttga aacggactgg cgggagctgg
     4561 agcgccaaag tccggcgaca tcactgttcc agagcttcga ctggctgcag agctgggccg
     4621 cggtctacct gacagagggc aacagcaccc agcccttcat cgtcaccggc acgctcggcg
     4681 gaaagctggt gttcgcctgg c
//
LOCUS       DBNGVR010000432         3788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_157483, whole genome shotgun sequence.
ACCESSION   DBNGVR010000432 DBNGVR010000000
VERSION     DBNGVR010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_157483"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC16A0_09195"
     CDS             <1..471
                     /locus_tag="AC16A0_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019565543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR3105551.1"
                     /translation="SVPDANVSWTEHAMVKHKHADIGVAVSIPGGLITPVIRKAETKG
                     LAQISAEMKDYAARARVKKLKPEEYTGGSAAVSNLGMMNVKNFAAIVNPPHATILAVG
                     TGERRPVVRGHEIVIEQQMTITLSTDHRCVDGALGAEFIGAIKMYLEEPGLMLV"
     gene            482..1120
                     /locus_tag="AC16A0_09200"
     CDS             482..1120
                     /locus_tag="AC16A0_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3105552.1"
                     /translation="MKTILAFGDSLTWGFRAGEWMRHDYADRWPNALQMGLGGKARVI
                     EEGHNGRTTVFDDPTTFDDRNGAKALPILLATHQPLDLVIIMLGTNDIKFANRCRAFD
                     ASLGMMRLVEIVQKFSFLPAYQTPQVLIMSPPPLVPTRDEWFNDLWGHAIAESKLFAK
                     HYADVAKTTGVGFFDAGTVATADPTDGGHLDAANTRAIGEALVPVVKSILKV"
     gene            1120..1827
                     /locus_tag="AC16A0_09205"
     CDS             1120..1827
                     /locus_tag="AC16A0_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105553.1"
                     /translation="MGASLFTLIKRLVLAMLAVAVLAAGVIYLFGPHLSVATAYAAYK
                     AAYDRRDGDAVARMTAPEGIAFFDVQRKRALTASRDTVAALSFRERGDVLSLRSQVLS
                     GSLPLDVLSNPDVTAAYATYVRHLAAKPSHLPAGILFAVPTGLDSARGYLDPAPGSGY
                     ALVTGLIVLAWGMHGDFEKTPEGWQVDITPALMSSASENERLAVRMEPTGNSYIYQLL
                     GAADAAAQDQLWQPLQQ"
     gene            1837..3276
                     /gene="lpdA"
                     /locus_tag="AC16A0_09210"
     CDS             1837..3276
                     /gene="lpdA"
                     /locus_tag="AC16A0_09210"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3105554.1"
                     /translation="MADTSFDIIIIGGGPGGYVAAIRAAQLGFKTAVVEREHLGGICL
                     NWGCIPTKALLRSAEIYHYMKHAKDYGLSAEKIGFDAAAVVQRSRGVSGRLNGGVTML
                     LKKNKVTVIWGEAKITKVGTVEVSKSSKPAMQPQIPEPKGAAGPGTYSAKNIIIATGA
                     RPRALPGLEPDGKLVWTYFEAMVPKVMPKSLLVVGSGAIGIEFASFYNAMGVDVTVVE
                     IMDQIMPVEDAEISKIAQKQLEKQGLKIRTGAKVSALKKAKDSVTATIEAGGKSEEIT
                     VDRVISAVGVQGNIEGLGLEALGVKTEKGCVVIDGYGRTNVPGIFAIGDVAGPPMLAH
                     KAEHEGVICVEKIKGLDVHPMKKEQIPGCTYCHPQVASVGLTEAKAKAAGFDLKVGRF
                     PFLANGKAIALGEDQGLVKTIFDAKTGRLLGAHMVGAEVTELIQGFVVAMGLETTEEE
                     LMHTVFPHPTLSEMMHESVLDAYGRVIHM"
     gene            3349..3630
                     /locus_tag="AC16A0_09215"
     CDS             3349..3630
                     /locus_tag="AC16A0_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3105555.1"
                     /translation="MNNLMDLGGGVGWIGTLFIGGLAGWIAEKIMKTDHGLLRNIIVG
                     ILGSIVGAFLANALGIRLNEIFQGWFVGNLIVSVIGAVVLLYVVRLIRR"
     gene            3649..>3788
                     /locus_tag="AC16A0_09220"
     CDS             3649..>3788
                     /locus_tag="AC16A0_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3105556.1"
                     /translation="MTGIGWLMTIILGGLAGWIAEKIMKFDTGLLMNIVLGIVGAVVG
                     NLL"
BASE COUNT          708 a         1141 c         1232 g          707 t
ORIGIN      
        1 tcggtgccgg acgccaatgt gtcctggacc gagcatgcca tggtgaagca caagcatgcc
       61 gatatcggcg tggcggtgtc cattcccggt ggcctgatca cgcctgtcat ccgcaaggct
      121 gagaccaagg gcctcgccca gatctcagcg gagatgaagg actatgcagc gcgggcgcgg
      181 gtgaagaagc tcaagccgga agaatacacc ggcgggtctg ccgccgtttc gaacctcggc
      241 atgatgaacg tgaagaactt cgccgccatc gtcaatccgc cgcatgcgac gatcctggcg
      301 gtcggtaccg gcgaacgccg tcccgtggtg cgcggccacg agatcgtcat cgagcagcag
      361 atgaccatca cgctgtcgac tgaccaccgc tgcgtcgatg gcgcgctggg cgcggaattc
      421 atcggcgcca tcaagatgta tctcgaagag cccggcctga tgctggtgtg aggagggcgc
      481 gatgaagacc atactggctt tcggcgactc tctgacctgg ggcttccgtg caggggagtg
      541 gatgcgccat gactatgcgg accgctggcc caatgccctc cagatggggc tgggcggcaa
      601 ggcgcgggtg atcgaggaag gccacaatgg ccgcacgacg gtgttcgacg acccgaccac
      661 cttcgacgac cgcaacggcg ccaaggccct gccgatcctg ctggcgacac atcagccgct
      721 cgatctcgtg atcatcatgc tgggcaccaa cgacatcaag ttcgccaacc gctgccgcgc
      781 cttcgacgcc tcgctcggca tgatgcggct cgtcgagatc gtgcagaagt tttccttcct
      841 gcccgcttac cagacaccgc aggtcctgat catgtcgccg ccgccgctgg tgccgaccag
      901 ggacgaatgg ttcaacgacc tgtggggcca tgcgattgcc gagtcgaaac tcttcgcgaa
      961 gcactatgcc gatgtggcca agaccacggg cgtgggcttt ttcgatgccg gtacggttgc
     1021 gacagccgat ccgaccgacg gcggccatct ggatgcggcc aacacgcggg ccatcggcga
     1081 ggcgctcgtc ccggtggtca agtcgatcct gaaagtctga tgggcgcttc gctcttcacc
     1141 ctgatcaagc gcctcgtgct ggcgatgctc gctgtggccg tgctggcggc gggcgttatc
     1201 tatctcttcg gtcctcatct cagcgtcgcc acggcctatg cagcctacaa ggcggcctat
     1261 gatcgccgcg acggtgacgc cgtcgcccgt atgacggctc ccgagggcat cgccttcttc
     1321 gatgtccagc ggaaacgtgc cttgaccgca tcgcgcgata ccgtggccgc gctgtccttc
     1381 cgcgagcgcg gggatgtgct cagtctgcga tctcaggtgc tgagcggcag cctgccactg
     1441 gatgtgctgt ccaatcccga tgtgaccgcg gcctatgcga cctatgttcg acaccttgcc
     1501 gcgaaacctt cgcacctgcc ggccgggatc ctctttgccg tgcccacggg tttagacagc
     1561 gcgcggggct atctcgatcc ggctcctgga agcgggtacg cgctcgtgac gggtctgatc
     1621 gttctggcct ggggcatgca tggcgacttc gagaagacgc cggagggatg gcaggtggac
     1681 ataacgcccg ccctcatgtc ctcagccagc gaaaacgagc ggctggccgt ccgcatggag
     1741 ccgaccggga attcctacat ctatcagttg ctgggcgccg ccgacgctgc cgcgcaagac
     1801 cagctctggc aaccgctgca acaatgagga cgaaccatgg ctgacacgag cttcgacatc
     1861 atcatcatcg gcggtggccc cggcggctat gttgcggcga tccgggccgc ccagctcggt
     1921 ttcaagacgg cggtggtgga acgcgagcac ctgggcggca tatgcctcaa ctggggctgc
     1981 atcccgacca aggcgctgct gcgctcggcc gaaatctacc actatatgaa gcatgccaag
     2041 gactacgggc tttcggccga gaagatcggc ttcgacgcgg ccgccgttgt gcagcgttcg
     2101 cgaggtgtgt cgggccgtct caatggtggc gtcaccatgc tgctgaagaa gaacaaggtc
     2161 acggtgatct ggggcgaggc gaagatcacc aaggtgggga ccgtcgaggt ctcgaagtcg
     2221 tcgaagcccg ccatgcagcc ccagattccg gagcccaagg gcgcagcggg gccggggacc
     2281 tattcggcca agaacatcat catcgccacc ggtgccaggc cgcgcgccct gccgggtctc
     2341 gagcccgacg gcaagctggt ctggacctat ttcgaggcca tggtgccgaa ggtcatgccg
     2401 aagtcgctgc tggtggtcgg ctcgggtgcc atcggtatcg agttcgcttc cttctacaat
     2461 gccatgggcg ttgacgtgac agtggtcgag atcatggacc agatcatgcc ggtcgaggat
     2521 gccgagatct ccaagatcgc ccagaaacag ctcgagaagc agggtctcaa gatccgcacc
     2581 ggcgcaaagg tctctgccct caagaaggcc aaggacagcg tcaccgcgac catcgaggcg
     2641 ggtggcaaga gcgaagagat cacggttgac cgggtgatct ccgccgtggg cgtccagggc
     2701 aatatcgaag gccttggcct agaggcgctc ggggtcaaga ccgagaaggg ctgcgtggtc
     2761 attgacggct atggccgaac caatgtgcct ggcattttcg ccatcggcga cgttgcaggc
     2821 ccgcccatgc tggcgcacaa ggccgagcac gagggcgtca tctgcgtcga gaagatcaag
     2881 ggtcttgacg ttcatccgat gaagaaggaa cagatccccg gctgcaccta ttgccatccg
     2941 caagtggcga gcgtgggcct gacggaggcc aaggccaagg cggcaggctt cgatctcaag
     3001 gtgggccgct tcccctttct ggccaacggc aaggccattg ccctgggcga ggaccagggg
     3061 ctggtgaaaa ccatcttcga cgccaagacg ggccgtttgc tgggcgctca catggttggc
     3121 gcggaggtga ccgaactgat ccagggtttc gtggtcgcca tggggctgga aacgaccgaa
     3181 gaagagctga tgcacacggt cttcccgcat ccgaccctct cggaaatgat gcatgagagc
     3241 gtgctggacg cctatggacg cgtcatccac atgtgagctt gggacttgat gaagggtcag
     3301 gggtgggcca tatcggtttc acctgattga cacaggaaag ggtgggacat gaacaatctg
     3361 atggatcttg gtggcggcgt gggctggatc ggtacgctct tcatcggcgg ccttgcaggc
     3421 tggattgcgg agaagatcat gaagaccgat cacggtcttc tgcgcaacat tatcgtgggc
     3481 atacttgggt ccattgtcgg cgccttcctg gccaatgcgc tgggcatcag gctgaacgag
     3541 attttccagg gctggttcgt cggcaacctc atcgtctcgg tgattggtgc cgtcgtgctg
     3601 ctctacgtgg tccggctcat ccggcgctga cacagaaggg agcatcacat gactggcatt
     3661 ggttggctca tgaccatcat cctcggcgga cttgccggct ggatcgccga gaagatcatg
     3721 aaattcgata ccgggctatt gatgaacatc gtgctcggga tcgtcggtgc cgtcgtcggc
     3781 aatctcct
//
LOCUS       DBNGVR010000433         2998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2908245, whole genome shotgun sequence.
ACCESSION   DBNGVR010000433 DBNGVR010000000
VERSION     DBNGVR010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2908245"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..617
                     /locus_tag="AC16A0_09225"
     CDS             60..617
                     /locus_tag="AC16A0_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HIR3105557.1"
                     /translation="MSQGVKLLIDMGPLILFFITNWKAGIFWGTGIFMLATAVALAVS
                     WALTRKIAMVPLASAVFVAVFGALTIWLQSDIFIKVKVTLINALFGAILLGGVMFGKN
                     YLKLVIGESLRMPEAAWRTLTIRWGVFFLFLALLNEIVWRNMSTDAWVNFKVFGLIPI
                     TLVFALANAPFMARHMIEDEPKSSA"
     gene            complement(601..1152)
                     /locus_tag="AC16A0_09230"
     CDS             complement(601..1152)
                     /locus_tag="AC16A0_09230"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3105558.1"
                     /translation="MSATSLPAHFRRKIPDGDTHQRDVCSTCGYVHYVNPKIVTGAIV
                     LHDGRILLCRRAIAPRIGFWTLPAGFMELGETAEQAAVREAREEANAEIAIDRLLAVY
                     TIPRIAQVQIMYLARLTSATISPGPESLEVVLAGWGEIPWAELAFPSVRWVLEQYQTV
                     QGRDDFAPFANPPGEFGDAKPMT"
     gene            1413..1913
                     /locus_tag="AC16A0_09235"
     CDS             1413..1913
                     /locus_tag="AC16A0_09235"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3105559.1"
                     /translation="MTASKDVKQFSRSPSHLLKRAAQFASHIYMAEVGKSGLTHRQYT
                     VLLAVDSNDGKSQTDLVKLTGIDRSTLADLVARLLAQGYLQRKRTKDDGRTNAIRITA
                     VGKKMLKMALPGADEVDRQLLSLVPSTDRRSFLDTLAVLATEMDKIEEREPAAPVRKI
                     RSRKRG"
     gene            complement(1929..2498)
                     /locus_tag="AC16A0_09240"
     CDS             complement(1929..2498)
                     /locus_tag="AC16A0_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR3105560.1"
                     /translation="MSEEPARGSRLWLAAIPVIAFLLLAAIFWRGLSGNPSEIPSALI
                     GKPVPSFTLPPVEGLGIPGFDAAALKTGQVTLVNVWASWCGPCRVEHPVLMELSKRSD
                     IRVFGLNYKDEPENARRFLGVLGQPYAAAVADESGRAAVDWGVYGVPETFIVDGDGII
                     RFKWIGPLTPEAVAGNFGAEIERAKAARP"
     gene            complement(2495..2653)
                     /gene="ccmD"
                     /locus_tag="AC16A0_09245"
     CDS             complement(2495..2653)
                     /gene="ccmD"
                     /locus_tag="AC16A0_09245"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR3105561.1"
                     /translation="MDFAAAHIGFVLASYGISFVSILGLAAYVIARDRKLRAEAQRLD
                     AARRREQT"
     gene            complement(2653..>2998)
                     /gene="ccsA"
                     /locus_tag="AC16A0_09250"
     CDS             complement(2653..>2998)
                     /gene="ccsA"
                     /locus_tag="AC16A0_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR3105562.1"
                     /translation="SMFVLFLLYIGYMAILQAIDEPHRAAMIARIVALVGFVNVPIVK
                     FSVDWWNSLHQPASVFRTDGPTNAPSMLWPLLVMGLAYSFLFLALHLTAMRAEIAARK
                     IRQIHIAETGVR"
BASE COUNT          606 a          916 c          920 g          556 t
ORIGIN      
        1 gacctgcaac cattcgcggc ggagggcttt gcccgcgcca ttgccggatt ggaggaccaa
       61 tgagccaggg cgtgaagctt ctcatcgaca tgggccctct gatcctgttt ttcatcacga
      121 actggaaggc cggcatcttc tggggcaccg gcatcttcat gctggcaacc gccgtggccc
      181 tggccgtctc ctgggcgctg acacgcaaga ttgccatggt tccgctcgct tcggcggtct
      241 tcgtcgcggt tttcggcgcg ctgacgatct ggcttcagag cgatatcttc atcaaggtga
      301 aggtcaccct gatcaacgcg cttttcgggg caatccttct tggcggtgtg atgttcggca
      361 agaattacct caagctggtg atcggcgaaa gcctgagaat gcccgaggcg gcctggcgca
      421 cacttaccat ccgctggggc gtcttcttcc tctttctggc gctgctcaat gagatcgtct
      481 ggcgcaacat gtcgaccgat gcctgggtga acttcaaggt cttcgggctg atccccatca
      541 ccctggtctt tgccttggcc aatgcaccct tcatggcgcg gcacatgatc gaggatgaac
      601 ctaagtcatc ggcttagcgt caccgaattc gcccggcgga ttggcgaagg gcgcgaagtc
      661 atcccggccc tggacagttt gatactgctc cagcacccag cggaccgacg gaaaggcgag
      721 ttcagcccac gggatctcac cccagcctgc gagcacgacc tcgagactct caggacccgg
      781 agagatcgtg gcggacgtga ggcgcgccag atacatgatc tggacctggg cgatgcgcgg
      841 aatggtgtag actgccagca accggtcaat ggcgatttct gcattggctt cctcgcgtgc
      901 ctcgcgcacg gcagcttgct ccgccgtttc tcccaactcc atgaagccgg ccggcagcgt
      961 ccagaagcca atacgcggcg caatggcacg gcggcacagc agaatccgcc cgtcatgcag
     1021 gacgatcgca cccgtaacga tctttggatt tacataatgg acatagccgc aggtactgca
     1081 gacatcgcgc tgatgcgtgt caccgtcggg gatctttcgt ctgaaatggg ccggcaggga
     1141 cgtggcgctc attgattgtc aagagttgaa ttccgtgttc tgatcatttt aactcggaag
     1201 cgtgatgact gatagtctag tttgcttggc ggcgtctgcc ctttttgctt gcagcagcag
     1261 ggacgagccg ctaggagagc gcggctccgg ggctggttgg cgggatttcc atgcaaggaa
     1321 gtcgaaagac cagagaggaa tgaagacgcc ggcgccgtga cctggagacc aggctgagcg
     1381 caggcgacac gaaggggcag gagaaggggt tcatgacggc atccaaggat gtgaagcagt
     1441 tttcgcgatc gccatcgcac ctgttgaagc gggcggcgca gttcgccagc cacatctaca
     1501 tggctgaagt cggcaagtcc ggactcaccc atcgccagta cacggtgctc ctcgcggtcg
     1561 acagcaatga cggaaagagc cagaccgacc tggtgaaact gaccggcatc gaccgatcga
     1621 cactggccga tctggtggcc cgcctcctcg ctcagggcta tctccagcgc aagcgcacca
     1681 aggacgacgg ccgcaccaat gcgatccgca tcaccgccgt cggcaagaag atgctcaaga
     1741 tggcgcttcc gggcgcagat gaagtggatc ggcagctcct gtcgctggtt ccttcgaccg
     1801 accgccggtc tttcctcgac acgctggccg ttcttgccac cgaaatggac aagatcgaag
     1861 agcgcgagcc cgctgcaccc gtccgcaaga tcaggtcacg caagcggggc tgattgccgg
     1921 caggccgttc agggtcttgc cgcctttgcc ctttcgattt ccgcaccgaa atttccagcc
     1981 accgcttccg gcgtaagcgg cccgatccat ttgaatcgga tgatgccgtc accatcgacg
     2041 atgaaggtct cgggcacgcc atatacaccc cagtcaacgg ccgccctgcc ggattcgtcg
     2101 gcaacggccg cggcgtaagg ctggcccagc acgccgagga agcgccgcgc attctcgggc
     2161 tcgtccttgt agttgagccc gaagacacgg atgtcgcttc gcttggagag ttccatcagc
     2221 accggatgct cgacccggca gggcccgcac catgaagccc agacattgac cagtgtcacc
     2281 tgccccgttt tcagagccgc agcgtcgaaa ccgggaatgc caagaccctc gacaggcggc
     2341 agcgtgaacg aaggcacggg ctttccgatc agtgcggaag gtatctccga cgggttgccc
     2401 gacagtcccc gccagaagat ggctgccaga agcaggaagg caatgaccgg aatggccgcg
     2461 agccagagac ggctgcccct tgccggctcc tcgctcatgt ctgttccctg cgacgggcgg
     2521 catccagacg ctgggcctcg gcgcgcaact tgcggtcgcg ggcaatcacg tatgcggcga
     2581 ggcccaggat agagacgaag ctgatgccat aggaggccaa cacaaatcca atatgggcgg
     2641 cggcgaagtc catcagcgca ctcccgtttc ggcaatatgg atctggcgga tcttgcgggc
     2701 ggcgatttcg gcacgcatcg ccgtcaggtg cagcgcgagg aacaggaagc tataggccag
     2761 tcccatcacc agcagcggcc acagcatgga gggcgcgttg gtcggaccat cggtccggaa
     2821 gacgctggca ggctgatgca gggaattcca ccagtcgacc gagaatttca cgatgggcac
     2881 attgacgaaa cccaccagtg ccacgatccg cgcgatcatt gcggcacggt gcggctcgtc
     2941 gattgcctgc aagatggcca tgtagccgat atagagaagg aacagcacga acatcgat
//
LOCUS       DBNGVR010000434         3709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_749999, whole genome shotgun sequence.
ACCESSION   DBNGVR010000434 DBNGVR010000000
VERSION     DBNGVR010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_749999"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /gene="mutL"
                     /locus_tag="AC16A0_09255"
     CDS             complement(<1..1504)
                     /gene="mutL"
                     /locus_tag="AC16A0_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3105563.1"
                     /translation="MAIRRLSEGTVNRIAAGEVIERPASVVKELVENAIDAGASAIEV
                     VFRSGGKSLIRVRDDGHGMDREDLLLAIERHATSKLPSDDLIEIRYLGFRGEALPSVG
                     AISRLSIASRPRGGGEAHEVTVEAGQVSGPRPVALSRGTEIEVRDLFFATPARLKFLK
                     TDRAESAEAADVVRRMALAYPHVGFSFVTEDRRVLDVPPGEKEEQRIRRILGNDFMEN
                     AVAFGLIREDVQAHGFAGLPTWTRAQGNMQYLFVNGRSVRDRQLVGAVRGAYADVMAA
                     NRFPAIVLFVTRPPDRVDVNVHPAKAEVRFRDPALVRSVIISAIRAALADRRPATDTT
                     LAQSMTSNFRPMSSSSVHAGFADVQRAFAMDMPIAASVSEDPPVADYPLGQARGQLHD
                     TYIVSQTRDGIVLIDQHAAHERLVYERLKRERAGRQLAIQPLLVPEVVDLDTAMVERL
                     SGAASVLAESGLVIEAFGDGAVIVREVPAAIAGGDISGIIRDVADELATFD"
     gene            complement(1534..2010)
                     /locus_tag="AC16A0_09260"
     CDS             complement(1534..2010)
                     /locus_tag="AC16A0_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105564.1"
                     /translation="MAILLAKGNAMKSRGSILGLLAIAVAASGCSSVSGLLDDGPAPQ
                     TMAAAPADLSMPPDLRLPPPGSAPPPAPAYEETQVVASAPAASVAAPAVPSTGVRKPE
                     DAAYEKYGIVIYNPDGTRRPDAVLREELRKATVAEKQKANPNYGTVFNIGNIFKDE"
     gene            complement(2025..2480)
                     /gene="lspA"
                     /locus_tag="AC16A0_09265"
     CDS             complement(2025..2480)
                     /gene="lspA"
                     /locus_tag="AC16A0_09265"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3105565.1"
                     /translation="MAALVFGFDQTNKWWMLFVYDISVHQPVEITSFFELVLVWNRGV
                     SYGLFSGHQQGFLIAMALVISVLLWVWASRSENRLGAWAFGLIIGGALSNALDRAVHG
                     AVADFFHFHWGSFSWYVFNIADVAIVAGVALLLYESLIDRNAGARRGNA"
     gene            complement(2525..>3709)
                     /locus_tag="AC16A0_09270"
     CDS             complement(2525..>3709)
                     /locus_tag="AC16A0_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3105566.1"
                     /translation="HAFCLEQRPDLKWPADLYLEGSDQHRGWFHSSLLESCGTRGRAP
                     YDAVLTHGFVVAEDGRKMSKSLGNIVTPQDVIKQSGADILRLWVASADYAEDLRLGPE
                     ILKTNVEAYRKLRNTMRYILGAVDGLKDWEFVPAAEMPELDRYILHRLHELEGQVRAA
                     YEVFDYKRLFALLTNFMTVDLSAFYFDIRKDSLYCDAWSSLRRRSCRTVLDQLFHCLS
                     TWWAPVLAFTMEEVWLARFGGAESSVHLMQFAQTPDEWGAPDLAAKWDKIRDVRSVVT
                     GALEIERQVKKTIGSALEAAPDVYVSRPDLIAALDGVDLAEISITSGARLIEGEGPAD
                     AFRLDSVKGVAVVFKPAEGRRCARSWKITPDVGSDPEFPDITPRDAEAVREWQIRFEK
                     TV"
BASE COUNT          776 a         1176 c         1116 g          641 t
ORIGIN      
        1 cgtcgaaggt cgcaagctcg tcggcgacat cgcgaatgat cccggagatg tcgccgccgg
       61 caatggcagc aggcacctcg cgcacgatga ctgcaccatc accgaaagcc tcgatcacca
      121 accccgactc ggccagtaca gacgctgcgc cggacaggcg ttcgaccatc gccgtatcaa
      181 ggtcaacaac ctccggcacc agcaggggct gaatcgccag ctgacggccc gcgcgctctc
      241 gcttcagtcg ctcatagacc aggcgctcat gcgctgcatg ctgatcaatg aggacaatgc
      301 catcccgggt ctgagacacg atgtaggtat catgcaactg gccgcgcgcc tggccaagtg
      361 gatagtccgc aacgggaggg tcttcggaga ccgatgccgc aatcggcatg tccatcgcga
      421 aggcgcgttg gacgtccgca aagcctgcat gtacggagga cgatgacatc ggtcgaaagt
      481 tcgaggtcat cgattgtgca agcgtggtgt ctgtcgccgg cctgcggtct gcaagggctg
      541 cccggatggc gctgatgatc accgacctta ccagagcggg atcgcggaac cggacctccg
      601 ccttggctgg atgaacgttc acgtccacac ggtccggcgg gcgggtgacg aagagtacaa
      661 tggcaggaaa gcggttggcc gccatcacat ccgcgtaggc gccccgcact gctccgacaa
      721 gctgtctgtc ccggacgcta cgaccattta cgaagagata ctgcatgttg ccctgagccc
      781 gcgtccaggt gggcaggcca gcaaagccgt gggcttgaac atcttcgcgg atcagaccaa
      841 aggcaaccgc attctccatg aagtcgttgc cgaggatcct gcggatccgc tgttcctcct
      901 tctcacccgg cgggacgtcg agaacccggc gatcctccgt gacgaatgag aagccgacat
      961 gcggataggc cagtgccatg cgtcgaacga cgtcggctgc ttccgccgat tcggccctgt
     1021 ccgtcttgag gaacttgagc cgggctggcg tggcgaaaaa cagatcgcgg acttcgattt
     1081 cggttcccct cgacagggca accggtcgcg gaccactgac ctgccccgcc tcgacggtga
     1141 cttcatgcgc ttcgccgccg ccgcgcggac gcgacgcaat gctcagccgg ctgatggcgc
     1201 cgaccgacgg cagggcttcg ccgcggaaac ccagatatcg aatctcgatc agatcgtcac
     1261 tcgggagctt cgacgtcgca tgacgttcga tggcgaggag aaggtcttcc cggtccatgc
     1321 catggccatc atcgcgaacg cggataaggc tcttgccgcc actcctgaaa acgacctcga
     1381 ttgccgaggc gccggcatcg atggcgttct ccacgagctc ttttacgacg cttgcaggac
     1441 gttcgatgac ttcgcctgcg gcgatccggt tgacggtgcc ttccgagagt cgcctgatgg
     1501 ccatgggtgc agcttcccgc gcggccccgg actctactcg tccttgaaga tattgccgat
     1561 gttgaacacg gtgccgtagt tcggattggc cttttgcttt tcggcgaccg tggccttgcg
     1621 cagctcctcg cgcaacacag cgtcgggacg gcgtgtaccg tccggattgt agatcacaat
     1681 gccatatttc tcgtatgccg catcttcggg tttacgcacg cctgtcgagg gtacggcggg
     1741 agcggccaca ctggctgccg gagccgacgc cacaacttgc gtctcttcat aggccggcgc
     1801 cggcggtggt gctgaaccag gcggcggaag acgaaggtcc ggcggcatgg aaagatccgc
     1861 aggggccgca gccatggttt gcggcgccgg accgtcgtcg agcaggccgc tgacagagga
     1921 acaaccggac gctgcaacgg ctattgccag cagacccaga attgaacccc gacttttcat
     1981 cgcattcccc ttggctaaca gaatggccat gtctggccgg aaattcaggc gtttccgcgg
     2041 cgggcccccg cattgcggtc aatcagcgat tcatacagca gcagcgccac cccggcaacg
     2101 atggcaacat cggcgatgtt gaagacgtac cagctgaagc tgccccaatg gaaatggaag
     2161 aagtcggcaa cggcaccatg caccgctcga tccagcgcat tggacagcgc accgccaatg
     2221 atcaggccaa aggcccaggc gccaagccgg ttctccgacc ggctcgccca gacccaaagg
     2281 agcaccgaaa tgaccagcgc catggcgatg agaaatccct gctgatggcc gctgaagagc
     2341 ccataggaaa cacccctgtt ccacaccagg accagctcga aaaacgatgt tatttcaacc
     2401 ggttgatgaa cagaaatgtc gtagacgaag agcatccacc acttgttggt ctgatcgaat
     2461 ccgaaaacca gtgctgccac cgccagaacc agcggcgcca atgggcccca caggtgcgac
     2521 cgactcaaac ggtcttctcg aagcggatct gccactcgcg cacagcttct gcatcgcgcg
     2581 gggtgatgtc cgggaactcc ggatcgctgc cgacatccgg cgtgatcttc caggacctcg
     2641 cacacctgcg gccctctgcg ggcttgaaga cgacagccac gcccttcacg ctgtccagcc
     2701 ggaaggcatc tgccggtcct tcgccctcga tcaggcgagc gccggaggtg atggatatct
     2761 ccgcaaggtc gacgccgtcc aaagcagcaa tcaggtctgg acgcgataca tagacatccg
     2821 gcgcggcctc gagtgccgaa ccgatcgtct tcttcacctg gcgctcgatt tcgagtgcgc
     2881 cagtgacgac cgaccgcaca tcacggatct tgtcccactt ggcggcaaga tcgggagcgc
     2941 cccactcatc gggcgtctgc gcaaactgca tcaggtgcac ggatgactcc gcaccaccga
     3001 agcgcgcgag ccagacctct tccatcgtga aagcgaggac aggtgcccac caggtggaga
     3061 gacaatgaaa cagttgatca agcacggttc tgcaggaacg tcgccgcagg ctggaccagg
     3121 catcgcaata gagggagtcc ttccggatgt cgaaatagaa agccgagaga tcaacggtca
     3181 tgaagttcgt cagaagcgca aagaggcgct tgtagtcgaa gacttcatag gccgcgcgga
     3241 cctgtccctc caattcatgc aggcgatgaa ggatatagcg gtcaagctcc ggcatctcgg
     3301 ccgcgggcac gaactcccag tccttcaggc cgtctaccgc acccaggatg tagcgcatgg
     3361 tattgcgcag cttgcgatag gcctcgacat tggtcttgag gatctcgggg ccgaggcgaa
     3421 ggtcctcagc atagtccgcc gaagcgaccc acaggcggag gatatcggca ccggattgct
     3481 tgatcacgtc ctggggggtc acgatgttac ccagcgactt cgacatcttg cggccgtcct
     3541 cggcgaccac gaaaccgtgc gtcagcacgg catcataggg cgcacgccca cgtgtgccgc
     3601 aggactcgag cagcgaggaa tggaaccagc cccgatgctg atcggacccc tcgagataga
     3661 gatcggcagg ccacttcaga tcaggccgct gctccaggca gaaggcatg
//
LOCUS       DBNGVR010000435         7902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4876336, whole genome shotgun sequence.
ACCESSION   DBNGVR010000435 DBNGVR010000000
VERSION     DBNGVR010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4876336"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="AC16A0_09275"
     CDS             complement(<1..886)
                     /locus_tag="AC16A0_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR3105567.1"
                     /translation="MGLLGLVAILAITLGAKADDLPFAIHMFIFAAAAILAVIMIARN
                     AHRPAASQAEYLDSVVRAGAIATVFWGVVGFLVGLVVALQLAYPAWNPNLEWFNFGRL
                     RPLHTSAVIFAFGGNALLCTSFYVVQRTCRARLALGNLAWFVFWGYQLFIVLAATGYL
                     LGVTEGREYAEPEWYIDIWITVVWVAYFLVFFGTLLKRKEPHIYVANWFYLAFIVTIA
                     MLHIVNNLSMPVSFTGYKSYSMFSGVQDALTQWWYGHNAVGFFLTAGFLGMMYYFVPK
                     QAERPVYSYKLSIIHFWAL"
     gene            1098..4610
                     /locus_tag="AC16A0_09280"
     CDS             1098..4610
                     /locus_tag="AC16A0_09280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3105568.1"
                     /translation="MFQDWTILLVGLGYLGLLFAVATWGDSQSRRRPRGGRPLIYALS
                     LGVYCTSWTYFGSVGIAARTGLDFIPIYLGPILVFVFGWRLIQAIAAISKRHNIASIA
                     DFISARYGKSEALGAVVAVIAVIGIVPYISIQLKADSASLQVINFAPLWQAGLPASAT
                     GIDGLSIMVAVTMGIFAILFGTRHIDTTEHQDGMILAIAVESIVKLVAFLLVGLFVVN
                     GIMGGWSSFSDRIAAAPQVEGLFAQGLDGGRWLTMTFLAACAIILLPRQFHVTAVENA
                     SGTEIRRARWLFPAYLVAINIFVIPIAIAGLLVMPPGSDPDTFVLALPVNAGSQIFTM
                     IAFIGGLSASTAMVIVECIALSIMVCNNLVVPLLLRQRDQERVAIHNDMGERLVLIRR
                     WAIVLVLALAYGYYRMIGASAALAQVGLISFAAIAQLAPAFFGGLLWRRGTARGAMAG
                     IAAGSAVWLYTLLLPSFVDAGWIGRGFIDDGPMGLAVLRPRMLFFMEFDPVTHGVLWS
                     MIVNIATYVVVSLMRLPTAIERMQAASFVSREIPGPGAGFRLWRTTVSAERLEATVAR
                     YFGPARARAAFAEYFAQRGGGSPVGQEADIRLFRFAENLLASAVGVSSARLVIALMLE
                     RHSTHARGAMRLLDDASAAIQYNRDVLQSAIDNVGQGIAVFDRDMSLICWNSQFQDYL
                     SLSSEMTRVGAPMAEIVPAFLARTDSRQEQSVDRLRKLMETHDLFRERMTNTGKVLEI
                     RSSSMPDGGVVVTFIDVTESVEAADALQRANESLERRVAERTAELTRLNKALAVAKSE
                     ADAANTSKTQFLAAASHDILQPLNAARLFAASLVERTRRTEESDLARNVDASLEAVED
                     ILSALLDISRLDAGAMKPDVAPFRIDEMLQALALEFTPAAARKNLHLKVMPCGLTVET
                     DRKLLRRVLQNLVANAIKYTRQGEVLMGCRRRGSKLRIEVHDTGPGIPPDKRGVIFQE
                     FQRLHGDDEQTGLGLGLSIVERIARVLDAPVTVRSTPERGSCFAIEVPVAAVGLPRLP
                     AARPQPLRTPGLPNAVTVLVIDNEKAILDGMETLLTGWGFIVHRARSQAEAVKALQAA
                     GGKLDMILADYHLVREDGISVVRALRKRARKHIPAVLITADRSPAVADSAVAHDIHIL
                     RKPVKPAALRAAMAHVAVRTEAAE"
     gene            complement(4611..5261)
                     /locus_tag="AC16A0_09285"
     CDS             complement(4611..5261)
                     /locus_tag="AC16A0_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3105569.1"
                     /translation="MGATRILIVDDHPLFREALKQAITGGVANAAIAQAGSLEAARAE
                     LDRHDTTDLVLLDLRMPGVQGLSGLIFLRAQYPNVPVVIVTASEDHGLVQKALNLGAS
                     GYIPKSSSTETIVDAINAVLRGDLWVPAGYDRKGQGDREAEEIARRIATLTAQQIRVL
                     MMLREGLLNKQIAYELNVSEATIKAHVSAILQKLNVSSRTQAVIAAGRIDGSVDSG"
     gene            complement(5285..5947)
                     /locus_tag="AC16A0_09290"
     CDS             complement(5285..5947)
                     /locus_tag="AC16A0_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105570.1"
                     /translation="MLTPLKSEVLKVDDLAHEARRFRTILWSFERNLELLLKDTGLVA
                     QVDHAALAEAFSRWRQGFDQTKHLADIDRHDFVIFAAGLMLKELIATNPITAVGEKAG
                     PLPELPAKIDHRLKRWPEGYAYTSFCLSVAAAVLNEMGDAEPQAARAADDPRFWDSFR
                     ENVAQDPAAAVAFFDMVCGLEPNWQSPDVPWMRRTFAEGFSRIATAKTAELDRPESLR
                     KD"
     gene            6076..>7902
                     /locus_tag="AC16A0_09295"
     CDS             6076..>7902
                     /locus_tag="AC16A0_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR3105571.1"
                     /translation="MSSTMSSDLKSGALVYHSEPKPGKLEIRATKPLGNQRDMALAYS
                     PGVAAVSEAIHADPLLVSRYTARANLVGVITNGTAVLGLGNIGPLAAKPVMEGKAVLF
                     KKFADIDVFDIELAETDVTRFVDTVAALEPTFGGINLEDIKAPECFEIEERLRQRMSI
                     PVFHDDQHGTAVIVGAAVVNALRLAKKDIAKVRIVASGAGAAALACLNQLVSLGAKRE
                     NIWVSDLEGVVYKGREALMDRWKAAFAQETDKRSLADVIAGADIFLGLSAGGVLKPGM
                     VKAMAPDPLILALANPNPEIMPEEATAIRPDAMICTGRSDYPNQVNNVLCFPYIFRGA
                     LDVGASTINEAMKRAAVNAIAELARETPSDVVARAYGGEARVYGKDSLIPAPFDPRLI
                     LRVAPAVARAAMDSGVATRPLADIDAYAESLQRFVFRSGFIMRQIFAAARQNPKRVIY
                     ADGEDERVLRAAQVVIEEGIAIPILIGRPAVVETRLKRHGLSIRLGADFDLINPEDDP
                     RYRDYVATYAEVAGRRGITPESARTVVRTSTTVIAALAVKRGEADAMICGLDGRYRAK
                     LRHIRDVIGLAPGVRQLSAMSLLITSHGIVFMTDTHVQSDPDA"
BASE COUNT         1305 a         2619 c         2553 g         1425 t
ORIGIN      
        1 tcagcgccca gaagtggatg atcgacagct tgtagctata gacggggcgt tcggcctgtt
       61 tcggcacgaa gtaatacatc atgccaagaa agcctgcggt caggaagaag cccaccgcgt
      121 tgtggccgta ccaccactgg gtcagcgcgt cctgcacgcc cgagaacatc gagtagctct
      181 tgtagcccgt gaacgagacc ggcatcgaga ggttgttgac gatgtgcagc atcgcgatgg
      241 tcacgatgaa ggcgaggtag aaccagttgg ccacgtagat gtggggttcc ttgcgcttca
      301 gcagggtgcc gaagaagacc aggaaatagg cgacccagac caccgtgatc cagatgtcga
      361 tgtaccattc gggttcggca tactcgcggc cctcggtcac gccgaggaga tagccggtgg
      421 cggccagaac gatgaagagt tggtagcccc agaagacgaa ccaggcgagg ttgccgagcg
      481 ccaggcgggc gcggcaggtg cgctggacga cgtagaagct ggtgcagagc agggcgttgc
      541 ccccgaaggc gaagatcacc gccgaggtgt gcaggggcct cagtcgtccg aagttgaacc
      601 actcgaggtt ggggttccag gccgggtagg ccaattgcag cgccaccacg aggccaacca
      661 ggaagccgac cacgccccag aagaccgtgg cgatggcgcc ggccctcacg acgctgtcga
      721 gatactccgc ctggcttgcg gcaggcctgt gggcgttgcg ggcgatcatg atcaccgcaa
      781 ggattgcggc ggccgcgaag atgaacatgt gaatggcgaa gggcaggtca tccgccttgg
      841 cgcccagagt gatggccagg atggcgacca gcccgagcag acccatctgc acgtatgatc
      901 ccgatcccga tccgtgtgta agtgttcccg accccatgat tgtcctcatt ctgcttggag
      961 tatgcgtcca caaatgttcc gccttctgtc cagagacatt gacgtggatc aaacgcgacg
     1021 ggtggcaatg tcgcggccgg cttggccaaa ggtcgaatcc cggttgattt tgcggcgtcg
     1081 caaagggatc atcggtcatg tttcaggact ggacaatcct gctcgtcggc ctcggctacc
     1141 tcgggctgct ctttgccgtg gcgacctggg gcgactcgca gtcgcggcgg cgcccgcgcg
     1201 gcgggcggcc cctgatttat gcgctttcgc tgggcgtcta ctgcacgtcc tggacctatt
     1261 tcggcagcgt cggcattgcg gcccgcaccg gactggactt catccccatc tatctggggc
     1321 cgatcctggt cttcgtcttc ggatggcggt tgatccaggc catcgccgcc atctcgaagc
     1381 gtcataacat cgcatccatc gcggacttca tctcggcgcg ctacggcaag agcgaggccc
     1441 ttggcgccgt ggtcgccgtc atcgccgtca tcgggatcgt gccctacatc tcgatccagc
     1501 tgaaggccga ctcggcctcg ctgcaggtga tcaacttcgc gccgctctgg caggccgggc
     1561 tgcccgcctc ggccaccggc attgatggcc tgtccatcat ggtggccgtc accatgggca
     1621 ttttcgccat cctcttcggc acccgccaca tcgatacaac cgagcaccag gatggcatga
     1681 tcctggccat cgcggtcgaa tcgatcgtga agctggttgc cttcctgctg gtcggcctct
     1741 tcgtggtgaa cggcatcatg ggcggctgga gcagcttttc cgaccgaatc gccgctgcgc
     1801 cgcaggtcga aggcctgttt gcgcagggcc tcgatggtgg ccgctggctg accatgacct
     1861 tcctggccgc ctgcgccatc atcctgctgc cgcgccagtt ccacgtcacg gccgtggaga
     1921 atgccagcgg caccgagatc cggcgcgccc gctggctgtt tccggcctat ctcgtggcca
     1981 tcaacatctt cgtcatcccc atcgccattg ctggcctgct ggtcatgccg cccggctcgg
     2041 atcccgacac tttcgtgctg gcgctgccgg tcaacgccgg cagccagatc tttaccatga
     2101 tcgccttcat cggcggactg tccgcctcca ccgccatggt gatcgtggaa tgcatcgcgt
     2161 tgtcgatcat ggtctgcaac aacctcgtgg tgccgcttct gctgcgccag agggaccagg
     2221 agcgcgtcgc cattcacaac gacatgggcg aacgcctggt gctcatccgg cgctgggcca
     2281 tcgtgctggt gcttgcactc gcctatggct actaccggat gatcggcgcc tcggcggcgc
     2341 tggcgcaggt gggcctcatc tcttttgctg ccattgcgca actcgcgcct gccttcttcg
     2401 gcggtctcct gtggcggcgc ggcacggcgc ggggcgcaat ggccggcatc gccgccggat
     2461 ctgccgtctg gctctatacc cttctgctgc cgtccttcgt cgatgccggc tggatcggcc
     2521 gcggcttcat cgacgatggc cccatgggcc tcgcggtgct caggccgcgc atgcttttct
     2581 tcatggaatt cgatccggtc acccacggcg tcctgtggag catgatcgtc aacatcgcca
     2641 cctatgtggt ggtctcgctc atgcgcctgc ccaccgccat cgagcgcatg caggcggcaa
     2701 gcttcgtgtc gcgcgagatc cccgggccgg gagcgggatt ccggctgtgg cggaccaccg
     2761 tttccgccga gcgcctggag gcaacggtcg cccgctattt cggtccggcg cgcgcgcgcg
     2821 ctgccttcgc cgagtatttc gcccagcgcg gcggcgggag cccggtgggg caggaggccg
     2881 atatccgcct cttccgcttt gccgagaacc tgctcgccag cgccgtcggg gtttcatctg
     2941 cgcggctggt gatcgcgctc atgctggagc gccactccac ccatgcgcgc ggtgccatgc
     3001 ggctgctcga cgatgcctcc gccgccatcc agtacaaccg cgacgtcctc cagtcggcca
     3061 tcgacaacgt gggccagggc atcgccgtct tcgaccggga catgtcgctg atctgctgga
     3121 acagccagtt ccaggactat ctgtcgctga gcagcgagat gacgcgcgtc ggcgcgccca
     3181 tggccgagat cgtcccggcc ttccttgccc gcaccgacag ccgccaggaa cagagcgtcg
     3241 accgcctccg caagctgatg gagacccatg acctgttccg cgagcggatg accaacaccg
     3301 gcaaggtgct ggaaatccgg tcaagctcga tgccggatgg cggcgtggtc gtcaccttca
     3361 tcgatgtgac ggaaagcgtc gaggccgccg atgccctgca gcgcgccaac gagtcgttgg
     3421 agcgccgcgt tgccgagcgc accgccgaac tgacacgcct caacaaggcg ctggcggtcg
     3481 cgaagtcgga agcagatgcc gccaacacgt cgaagaccca gttcctggcc gcggcaagcc
     3541 atgacatcct gcagccgctc aatgcggccc ggctcttcgc ggctagcctg gtggagcgca
     3601 cgcggcggac cgaggaatcc gacctggccc gaaatgtcga tgcctcgctc gaagcggtgg
     3661 aagacattct gtccgcgctg ttggatatct cgcgcctcga tgccggcgcc atgaagccgg
     3721 atgtggcgcc cttccgcatc gatgagatgc tgcaggccct tgcgctcgag tttacgccgg
     3781 ccgccgcgcg gaagaatctc cacctcaagg tgatgccctg cgggctcacc gtcgagaccg
     3841 accgcaagct cttgcgccgg gtgctgcaga acctggtggc caatgccatc aagtatacgc
     3901 gtcagggcga agtgctcatg ggctgccgcc ggcgcggatc gaaactgcgc atcgaggtgc
     3961 atgacacggg tcccggcatt ccgccagaca agcgcggcgt gatcttccag gaattccagc
     4021 gcctgcacgg cgacgatgag cagacgggac tggggctcgg gctgtcgatc gtcgagcgca
     4081 tcgcccgcgt gctggacgcc ccggtcacgg ttcgctcgac gcccgagcgc ggcagctgct
     4141 tcgccatcga ggtgccggtg gccgcggtgg gcctgccgcg gcttccggca gccaggccgc
     4201 agccgctccg cacaccgggc ctgcccaatg ctgtcaccgt tctggtcatc gacaacgaga
     4261 aggccatcct cgacggcatg gaaacactgc tcaccggctg gggcttcatc gttcaccggg
     4321 cgcgcagcca ggccgaggcg gtcaaggccc tgcaagccgc cggcggcaag ctcgacatga
     4381 tcctcgccga ctatcatctg gtacgcgagg atgggatctc ggtcgtacgt gccctgcgca
     4441 agcgggcccg caagcacatt cccgccgtgc tcatcaccgc cgaccgctcg cccgccgtgg
     4501 ccgacagcgc ggttgcgcac gatatccaca tcctgcgcaa gccggtgaaa ccggcggcgc
     4561 tgcgcgccgc catggcgcat gtcgcggtcc ggaccgaagc tgcggagtga tcagccggag
     4621 tccacgctgc cgtcgatgcg gccggcggca atcaccgcct gggtgcggct tgagacgttg
     4681 agtttctgca ggatcgccga cacatgcgcc ttgatggtgg cttccgagac gttgagctca
     4741 taggcgatct gcttgttgag caggccctcg cgcagcatca tcaggacgcg gatctgttgc
     4801 gcggtgagcg tggcgatgcg gcgggcgatc tcttcggcct ccctgtcgcc ctgtcccttg
     4861 cggtcgtatc ccgccggaac ccagaggtcg ccgcgcagca cggcattgat ggcatcgacg
     4921 atggtctccg tgctggagga tttcggaata tagcccgagg cgccgaggtt cagcgccttc
     4981 tgcaccaggc catggtcctc ggatgcagtc acgatgacga cgggcacgtt ggggtattgg
     5041 gccctcagga agatcaggcc cgagaggcct tgcacgcccg gcatgcgcag gtcgagcagg
     5101 acgagatcgg tcgtgtcatg gcggtcgagc tcggcgcgcg cggcctccag cgatccggcc
     5161 tgggcgatgg cggcgttggc gacgccgccg gtaatcgcct gcttcagggc ctcgcggaac
     5221 aggggatgat cgtcgacgat cagtatgcga gttgccccca agctccggcc ctccctgcct
     5281 tgcctcaatc tttgcgcaag ctttccggtc gatcaagttc cgctgtcttg gccgtggcga
     5341 tgcggctgaa accctccgca aatgtccggc gcatccaggg cacatcgggc gactgccagt
     5401 tcggttcgag tccgcagacc atgtcgaaga aggccacggc cgcggccggg tcctgggcca
     5461 cgttctcgcg gaaggagtcc cagaagcgcg gatcgtcggc agcacgcgct gcctgcggct
     5521 cggcatcgcc catctcgttc agcaccgcag cggcaaccga aaggcagaag cttgtgtagg
     5581 catagccttc cggccatcgc ttgagacggt gatcgatttt ggcgggaagt tcgggcaggg
     5641 ggccggcctt ctcgcccacc gcggtgatcg ggttggtggc aatcagctcc ttgagcatga
     5701 gcccggccgc gaagatcacg aagtcgtgcc ggtcgatatc ggccaggtgc ttggtctggt
     5761 cgaaaccctg gcgccagcgc gaaaaggcct cggcgagcgc tgcatggtcc acctgggcga
     5821 cgagtcccgt gtccttcagc agcaactcca gattgcgctc gaaagaccac aggatggtgc
     5881 ggaaccttcg cgcctcatgc gcgaggtcgt ccaccttcag cacttctgat ttcaaaggcg
     5941 taagcatggc atctcacgtt ctcgtatcat tctatcccgg ctggctgcaa ggtccagtac
     6001 ggaaatccgt ccacgtctgg ttgttgcaaa ttccggcaaa tcacggtcat ttgcccgggg
     6061 cttaaggagt tctcgatgtc gtccaccatg tccagcgacc tgaagtcggg cgcgctcgtc
     6121 taccattccg aaccgaagcc gggaaagctg gaaattcgcg ccaccaagcc gttgggcaat
     6181 cagcgcgaca tggcgctggc ctattcgcca ggcgtggcgg cggtgtcgga ggccatccat
     6241 gcggatcccc tgctggtgtc gagatacacg gcccgcgcca atctcgtggg cgtcatcacc
     6301 aacggcacgg ccgtgttggg gctgggcaac atcgggccac tggccgccaa gccggtgatg
     6361 gagggcaagg cggtgctctt caagaagttc gccgacatcg acgtcttcga catcgagctg
     6421 gccgagacgg acgtcacccg cttcgtcgac accgtggcag cgctcgagcc caccttcggc
     6481 ggcatcaatc tcgaggacat caaggcgccg gagtgcttcg agatcgagga gcggctgcgc
     6541 cagcgcatgt ccattccggt cttccatgac gaccagcacg gcacggccgt catcgtcggc
     6601 gcggcggtgg tcaatgctct gcggctcgcg aagaaggaca tcgcgaaggt caggatcgtg
     6661 gcctccggcg ccggggccgc ggcacttgcc tgcctcaacc agctcgtcag cctgggcgcg
     6721 aagcgggaga acatctgggt ctcggatctc gagggcgtcg tctacaaggg ccgcgaggcg
     6781 ctcatggacc gctggaaggc cgcctttgcc caggagaccg acaagcggag cctcgccgac
     6841 gtgatcgccg gcgccgacat cttcctcggc ctctcggcgg ggggcgtgct gaagcccggc
     6901 atggtcaagg ccatggcgcc ggatccgctg atcctggcgt tggccaatcc caatccggaa
     6961 atcatgccgg aggaggcgac cgcgatccgc cccgatgcca tgatctgcac cggccgctcg
     7021 gactatccga accaggtcaa caacgtgctg tgctttcctt acatcttccg cggcgccctg
     7081 gatgttggcg cgtcgaccat caacgaggcg atgaagcggg ccgcggtgaa cgccatcgcc
     7141 gagctggcgc gcgagacgcc gtccgatgtt gtggcccgcg cctatggcgg cgaggcccgc
     7201 gtctacggca aggactcgct catccccgcg cccttcgatc cgcgcctcat cctgcgggtg
     7261 gcgccggcgg tggcgcgtgc ggccatggac agcggcgtcg ccacgcgccc gctggccgac
     7321 atcgacgcct atgccgaaag cctgcagcga ttcgtcttcc gctccggctt catcatgcgg
     7381 cagatcttcg ccgccgcgcg ccagaacccg aagcgcgtca tctatgccga tggcgaggac
     7441 gagcgcgttt tgcgcgccgc ccaggtggtg atcgaggaag ggatcgccat tcccatcctc
     7501 atcgggcgtc cggccgtggt cgaaacgcgc ttgaagcgcc atggcctgtc gatccgcctt
     7561 ggcgcagact tcgacctcat caacccggaa gacgatccac gctaccgcga ttacgtggcg
     7621 acctatgccg aagtggcggg acgccgtggc atcacgcccg aaagtgcccg gactgtggtt
     7681 cgcacctcga ccacggtgat tgctgccctg gcggtcaagc gcggcgaggc ggatgcgatg
     7741 atctgcggcc tcgacggccg ctaccgcgcc aagctccgcc atatccgcga tgtcatcggg
     7801 ctggctcccg gtgtgcgcca gctctccgcc atgagcctgc tcatcacctc gcatggcatc
     7861 gtgttcatga ccgacaccca tgtgcagtcc gatcccgatg cc
//
LOCUS       DBNGVR010000436         1935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_357397, whole genome shotgun sequence.
ACCESSION   DBNGVR010000436 DBNGVR010000000
VERSION     DBNGVR010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_357397"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="AC16A0_09300"
     CDS             complement(<1..870)
                     /locus_tag="AC16A0_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HIR3105572.1"
                     /translation="MFQSLVHMARLFRAGRTLARYDVLLQPQQLADLPWPARLGVKLM
                     RFGTGSPATDPQSRLTAALSALGPSYIKLGQFLATRPDIIGPQRAFELKALQDRLPPF
                     GMDEARAIIAANIGPVDTQFTELSAPVAAASIAQVHQGRTTDGRLVAVKVLRPGVEKR
                     FAADLQSFYFAARLLERFSAEGRRLRPVAAVAMLEDSMKLELDLRMEASAMSEIAENT
                     RDDPGFRVPAVDWGRSARQVLTTGWIDGTPIADVAALQAQGFDLKQLADTVIQSFLRQ
                     AIRDGFFHADMHPG"
     gene            complement(870..1634)
                     /gene="ubiE"
                     /locus_tag="AC16A0_09305"
     CDS             complement(870..1634)
                     /gene="ubiE"
                     /locus_tag="AC16A0_09305"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR3105573.1"
                     /translation="MTDDRKTDVDFGFRRVGEAEKQGLVNEVFSKVAERYDQMNDLMS
                     AGLHRLWKDDFVAMLNPPRGKTAFSVLDVAGGTGDIALRIARRGGHGTHVTVADISPE
                     MVAEGRKRAEREGLLARCDFTVGNAEALAFPDRSFDAYTIAFGIRNVTHIDRALSEAY
                     RVLKPGGRFMCLEFSQVDVPLLDSIYDAYSFTVIPAVGKVVTGDGHPYRYLVESIRTF
                     PKQEKFRDMISAAGFANAIYRNLTGGVVAIHSGWRI"
     gene            complement(1639..>1935)
                     /locus_tag="AC16A0_09310"
     CDS             complement(1639..>1935)
                     /locus_tag="AC16A0_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3105574.1"
                     /translation="WVNAFQLAFTLNRRGHFALDQRLRQRLPRIVLAALAMGALLLAG
                     KHLLAGNYAEGAGFLAALWGLALLVAGGISSYFLLAHVTGAMKIGELKAMLKRS"
BASE COUNT          344 a          621 c          659 g          311 t
ORIGIN      
        1 gccggggtgc atgtcggcgt gaaagaaacc gtcgcggatc gcctgccgca ggaaactctg
       61 gatcaccgtg tcggccaact gcttcaggtc gaagccctgg gcctgcaagg ccgcgacatc
      121 ggcgatcggc gtgccgtcga tccagccggt ggtcagcacc tggcgcgcac tgcggcccca
      181 gtcgacggct ggcacgcgga atccaggatc gtccctggtg ttctcggcga tctccgacat
      241 ggcggatgct tccatgcgca ggtcgagttc cagtttcatc gagtcttcca gcatggccac
      301 ggccgccacg ggtcggaggc ggcggccctc ggcgctgaag cgctccagca ggcgcgcagc
      361 gaagtagaag ctctgcaggt cggcagcgaa gcgcttttcg acgccaggcc gcagcacctt
      421 caccgccacg aggcggccgt cggtggtacg gccctgatgt acctgcgcga tggaggccgc
      481 agccacgggt gccgagagtt cggtgaactg cgtgtccacg gggccgatgt tggcggcgat
      541 gatcgccctt gcctcgtcca tgccgaaggg cggcaggcga tcctgcagcg ccttgagctc
      601 gaaggcgcgc tgcggcccga tgatgtcggg ccgggtcgcg aggaactgcc cgagcttgat
      661 gtagctaggc cccaacgcgg agagcgctgc tgtcaggcgg ctctgcggat cggttgccgg
      721 cgatccggta ccgaaccgca tcagcttcac gccgaggcgc gccggccagg gcaggtcagc
      781 caactgctgc ggctgcagca gcacgtcgta gcgggcgagt gtccgcccgg cccggaacag
      841 cctcgccatg tgcacaaggc tctggaacat cagatgcgcc agccggaatg aattgccacg
      901 acgccgccgg tgaggttgcg gtagatcgcg ttggcgaagc ccgccgcgga aatcatgtcg
      961 cggaacttct cctgtttcgg gaaggtgcgg atcgattcca ccagatagcg gtagggatgg
     1021 ccgtcccccg tcaccacctt gccgacggcg ggaatgacgg tgaaggaata ggcatcgtag
     1081 atcgaatcga gcaacggcac gtcgacctgg gagaactcga gacacatgaa gcggccgccg
     1141 ggcttcagca cgcggtaggc ttcgctcagg gcgcggtcga tatgggtcac gttgcggatg
     1201 ccgaaagcga tcgtataggc atcgaagctg cgatcgggaa aggccagtgc ctcggcattg
     1261 cccacggtga agtcgcagcg cgccaggagg ccttcgcgtt ccgcccgctt acggccctcg
     1321 gccaccatct cgggcgaaat gtcggcgacg gtcacatggg tgccatgccc cccgcgccgc
     1381 gcgatgcgca aggcaatgtc gccggtgccg ccggcaacgt cgaggacgct gaaagccgtt
     1441 ttcccccgcg gcggattgag catcgccacg aagtcgtcct tccatagccg gtgcagcccc
     1501 gcggacatga ggtcgttcat ctggtcatag cgctcggcaa ccttggagaa gacctcgttg
     1561 acgaggccct gcttctcggc ctcgccgacg cggcggaagc cgaagtcgac atcggtcttg
     1621 cggtcgtccg tcatgggtct agctccgctt cagcatggcc ttcagctcgc cgatcttcat
     1681 ggcgccggtg acatgggcca gcaggaaata gctggatatg ccgccggcaa cgagaagcgc
     1741 aagcccccat aaggccgcaa ggaagcctgc tccttcggca tagttaccgg ccagaagatg
     1801 cttgccggcc agcagcagcg cgcccatcgc gagcgcggcc agcacgatcc gcggcaggcg
     1861 ctggcgcagc cgctggtcaa gggcgaagtg gccgcgccga ttcagggtaa atgccagctg
     1921 aaaggcattt accca
//
LOCUS       DBNGVR010000437         6552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4284977, whole genome shotgun sequence.
ACCESSION   DBNGVR010000437 DBNGVR010000000
VERSION     DBNGVR010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4284977"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC16A0_09315"
     CDS             <1..497
                     /locus_tag="AC16A0_09315"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3105575.1"
                     /translation="NHYVGRTFIEPTQTIRSLGVKLKHNANRAVVKDKRVVLVDDSIV
                     RGTTSVKIVRMMYEAGAKEVHMRISSPPIAHPDFYGIDTPEQKSLLAATHTLEQMRDY
                     IGVASLAFLSVDGIYRAVGLPGRDNVRPQFTDHCFTGDYPTPLTDLVGEHGKQTLSLF
                     AEAG"
     gene            501..1235
                     /locus_tag="AC16A0_09320"
     CDS             501..1235
                     /locus_tag="AC16A0_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3105576.1"
                     /translation="MSRLLENRIAVVTGASRGLGRAMSLALAREGAHIVATARTQGGL
                     EELDDEIKAIGGSATLVPCDIKDFDALDRLGAAIFERWKKLDIFVGNAGTLGKLTPLP
                     HLDPKVWDDAMAINVTANYRLIRSLDPLLRAADAGRVVLVTSGLAHKCWPYWGPYSIS
                     KAALEAMAKVYAGETATTAVRVNCFSPGPTRTKMRATAMPGEDPETLPSAEEVAAQMV
                     AMCTPAFTDTGTVWKYASTGLFKQFG"
     gene            1232..1999
                     /locus_tag="AC16A0_09325"
     CDS             1232..1999
                     /locus_tag="AC16A0_09325"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3105577.1"
                     /translation="MSDDAIITAFAEARRSEVHLAELPEPAPPAMDRAFRLQQAVTAA
                     LGWEQCGWKIGCTSLRAQKALNADGPFPGTMFAHRVFKSGDHVPTIPSNKRVVEPEVA
                     FVMAKSLPPRATPYARDDVLSAVAAVHAAIEVVNPRTPKGFADPVPWFVVDGGLNDSI
                     VLGPAVKPLAPDAYARLAARVSWNGRDMQGGVGSNALGGGDLALTWLANHLVAHGAGL
                     KEGDIITTGVITEFFSAAPGDLIEASFEGLGEVALRF"
     gene            complement(2000..3340)
                     /gene="der"
                     /locus_tag="AC16A0_09330"
     CDS             complement(2000..3340)
                     /gene="der"
                     /locus_tag="AC16A0_09330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3105578.1"
                     /translation="MTLTVAILGRPNVGKSTLFNRLVGRKVALVDDQPGVTRDRREGD
                     ARIGDLKFKVFDTAGLDEARRDTLEARMSAQAETAIGDADVVLFVIDARAGVTPTDKQ
                     FAERIRRKGKPVILVANKAESRAADAGLLEAFELGFGKPLAVSAEHGEGLDQIYDALL
                     PHRDDVEDDGGEDRADGPLRLAIIGQPNAGKSTLVNALLGEERMLTGPEAGITRDAIS
                     SDFEWKGQRIRLWDTAGIRRKAKVTGKIEKLAVSDALRAIRFAECVIVLIDASMPIES
                     QDLTLANLVSEEGRAIVLALSKWDIVEDKQKTMKEVESKLEDVLPEIRGVPVVTLSAK
                     QERGLDKLLNAVLGAMEKWNSRISTAQLNRWLEAAIERNPPPAPSGRRIKIRYATQAS
                     SRPPTFAIFGNQLARLPTSYTRYLMNGLRKDFELWGVPIRFSLRGGKNPFDKDR"
     gene            complement(3337..3627)
                     /locus_tag="AC16A0_09335"
     CDS             complement(3337..3627)
                     /locus_tag="AC16A0_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105579.1"
                     /translation="MRKLAMLALAGLMLSGCSYVDRLTGETDNTVLPGAREDAVPGRA
                     QFPDPSERAQTASGGGSVGAPSDASTVGSDPGCPPEEPNCGAGSGDVFADPQ"
     gene            complement(3633..4289)
                     /locus_tag="AC16A0_09340"
     CDS             complement(3633..4289)
                     /locus_tag="AC16A0_09340"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3105580.1"
                     /translation="MTDESLFREVDEEVRQEQYKKIWSQYGNIFIALCLLIVLGVAGY
                     KGWQYWQKTQAEAASVVFFQAVANGNAGKSEEALKEFESIGHTGYAQLAKLREAGVLA
                     ASGKTDEAIKLYDTIAASAGTAAPLRDLARLRAAYVSIDSASFADISARVKDFDTAGN
                     PWRHMAREILMLSAFKAGDHAGADSQAQAILGDPEAPAPLRQRAGMIADLLLPILGQK
                     "
     gene            complement(4336..4491)
                     /locus_tag="AC16A0_09345"
     CDS             complement(4336..4491)
                     /locus_tag="AC16A0_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105581.1"
                     /translation="MAMELAGFLFGSLMVGGLAYAYISHERWKTIRAVYARDGDRRRN
                     ALIWPSR"
     gene            complement(4563..5939)
                     /locus_tag="AC16A0_09350"
     CDS             complement(4563..5939)
                     /locus_tag="AC16A0_09350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3105582.1"
                     /translation="MSFTRRQILAAAASLAVLVPGQAPRADDFIELRARNAELGLLEG
                     GAGKTGAWILGKGPAPAVIRAKQGQELKLRIFNELDNEIWLHFFGLRGPSELMTINVP
                     PGDKGVDCVFTPPDAGTFWIGPVSDVSRHRDMGLYAVLVVEEKEALDPAIPDLPLIID
                     DWRIAQDGTMEGGFGDIETMVGEGRLGNWMTINSQFRPRLPIAAGRFTRLRILNAANV
                     RSMGLQFKGSDPLLIAFDGQPVKPRLLGTKALTLAPGQRADLLVSAEEGDITIALDLF
                     EDVVEIGYLTRDGEAAPAAIDDNFALPPNPVTRPPAKPEPRLVKIALEGGVKGGLKSA
                     VFRGQTMELRTLLENGKGWALNGVAGPGIEPLFAAQLGESLALEIENRTAFSQALHLH
                     GHVWQLPAEEGSEPPVSDTAVVPAGQTKLLGFVADNPGTWAFQSLVAERSDGGLIGAF
                     QVVKPDQL"
     gene            complement(6027..>6552)
                     /gene="panB"
                     /locus_tag="AC16A0_09355"
     CDS             complement(6027..>6552)
                     /gene="panB"
                     /locus_tag="AC16A0_09355"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3105583.1"
                     /translation="SQAFHNAAKVIKETQCGAIKLEGGRRMAETIHFLTERGIPVMAH
                     IGLTPQSINILGGFRTQGRTREQWALIEEDARVVTEAGAFSVVLEAIAEPLSAKITRQ
                     IPIPTIGIGASAACDGQILVMEDMLGLSPRVPKFVKEFGKVGAAIEGAIRMYADDVRS
                     RAFPQPENTYDMKD"
BASE COUNT         1093 a         2188 c         2112 g         1159 t
ORIGIN      
        1 gcaaccatta cgtgggccgc accttcatcg agcccacgca gaccatccgc tcgctgggcg
       61 tgaagctcaa gcacaacgcc aaccgcgccg tggtgaagga caagcgcgtg gtgctggtcg
      121 acgattcgat cgtgcgcggc acgacctccg tcaagatcgt gcgcatgatg tatgaggcgg
      181 gtgcgaagga ggtgcacatg cgcatctcct caccgcccat cgcacatccg gatttctacg
      241 gcatcgacac gccggaacag aaatcgctgc tggccgccac tcatacgctg gaacagatgc
      301 gcgactatat cggcgtggcg tcgctcgcct tcctctcggt cgatggcatc taccgcgcgg
      361 tgggcctgcc cggccgcgac aatgtccgcc cgcaattcac cgaccactgc ttcaccggcg
      421 attatcccac cccgctcacc gacctggtgg gcgagcatgg caagcagacc ctgtcgctct
      481 tcgcggaggc cggctgatcc atgtcgcgcc tgcttgaaaa ccgcatcgcc gtggtcaccg
      541 gcgcatcgcg cggccttggc cgtgccatgt cgctggccct tgccagggaa ggcgcgcata
      601 tcgtcgccac ggcccggacc cagggcggcc tcgaggaact cgacgacgag atcaaggcga
      661 tcggcggcag cgccacgctg gtgccgtgcg acatcaagga tttcgatgcg ctcgaccgat
      721 tgggtgcggc catcttcgag cgctggaaga aactcgacat cttcgtgggc aatgccggca
      781 cgctgggcaa gctgacgccc ctgccccatc tcgaccccaa ggtctgggac gatgccatgg
      841 cgatcaacgt taccgccaat taccggctga tccgctcgct cgatcctctg ctgcgcgccg
      901 ccgacgcggg ccgcgtcgtg ctggtgacgt cgggccttgc ccacaagtgc tggccctatt
      961 gggggcccta ctccatttcc aaggccgcgc tcgaggccat ggccaaggtc tatgccggcg
     1021 agacggcaac cactgcggtg cgcgtcaact gcttcagtcc ggggcctaca cggaccaaga
     1081 tgcgcgcaac cgccatgccc ggcgaggatc ccgagaccct gccctcggca gaagaagtgg
     1141 ccgcgcagat ggtggccatg tgtacgccgg ccttcaccga cacgggcacg gtgtggaaat
     1201 atgcgtccac cggtctcttc aagcagttcg gatgagcgac gacgccatca tcaccgcctt
     1261 cgccgaagca cgccgcagcg aagtccatct ggcggaactg ccggaacccg cgccgcccgc
     1321 gatggaccgc gccttccgtc tgcagcaggc ggtcaccgcc gcgttgggct gggagcaatg
     1381 cggctggaag atcggctgca ccagcctgcg cgcccagaag gcgctgaatg cggacggccc
     1441 ttttcccggc accatgttcg cccaccgcgt gttcaagtcc ggagaccatg tgccgacgat
     1501 cccgtccaac aagcgggtgg tggaacccga ggtggccttc gtgatggcga agtcgctgcc
     1561 gccgcgcgct acgccctatg cgcgggacga cgttctgtcg gcagtggcgg ccgtgcatgc
     1621 cgcgatcgaa gtggtcaatc cgcgcacgcc gaagggcttc gccgatccgg tgccgtggtt
     1681 cgtggtcgat ggcgggctga acgactcgat cgtgctcggt ccggcggtga agccgcttgc
     1741 accggacgcc tatgcccggc ttgcggcccg ggtgagctgg aacggccgcg acatgcaggg
     1801 cggtgtcggc agcaatgccc tgggcggcgg cgatctggcg ctgacctggc ttgccaatca
     1861 cctcgttgcg catggcgcag gcctgaagga aggcgacatc atcaccaccg gcgtgatcac
     1921 cgaattcttc tcggctgctc ctggcgactt gatcgaggct tccttcgaag gcctgggcga
     1981 ggtcgcgctg cggttctgat cagcggtcct tgtcgaaggg gttcttcccg ccgcgcaggc
     2041 tgaagcggat gggcacgccc cagagttcga agtccttgcg caggccgttc atgagatagc
     2101 gcgtgtagct ggtgggcagg cgcgccagct ggttgccgaa gatggcgaag gtgggcggcc
     2161 gcgaactggc ctgggtggcg tagcggatct tgatgcggcg ccccgacggt gcgggcggcg
     2221 gattgcgttc gatcgccgcc tcgagccagc ggttgagctg ggcggtggaa atgcggctgt
     2281 tccatttctc catggcgccg agcaccgcgt tgagcagctt gtccagtccg cgctcctgct
     2341 tggccgacag cgtcaccacc ggcacgccgc ggatctcggg cagcacgtcc tcgagtttgc
     2401 tttcgacctc cttcatggtc ttctgcttgt cctcgacgat gtcccacttg ctgagggcga
     2461 ggacgatggc gcggccctct tcagagacga gattggcgag cgtcagatcc tgactctcga
     2521 tcggcatcga ggcatcgatc agcacgatca cgcattcggc gaagcggatg gcgcgcaagg
     2581 cgtcggagac cgccagcttc tcgatcttgc ccgtgacctt ggccttgcgg cggatgccgg
     2641 ccgtgtccca cagccggatg cgctgcccct tccactcgaa gtcggacgag atcgcatcgc
     2701 gcgtgatgcc ggcttcgggc ccggtcagca tgcgctcctc gccgagcaag gcattcacca
     2761 gcgtcgactt gccggcattg ggctggccga tgatggcaag gcgcagcgga ccatcggcac
     2821 ggtcctcgcc gccatcgtcc tcgacgtcgt cgcgatgggg cagcagcgca tcgtagatct
     2881 ggtcaaggcc ttcgccatgc tcggcggaaa ctgccagcgg cttgccgaat cccagttcga
     2941 aggcttccag caggcccgca tcggcggcac ggctctccgc cttgttggca acaagaatga
     3001 caggcttgcc cttgcgccgg atgcgttcgg cgaactgctt gtcggtgggt gtcacgccgg
     3061 cacgcgcgtc gatgacgaag agcaccacat cggcgtcgcc gatggcggtc tcggcctggg
     3121 cactcatgcg ggcttccagc gtgtcgcgcc gcgcctcgtc aaggcctgcc gtgtcgaaga
     3181 ccttgaactt caggtcgccg atgcgggcgt caccctcacg gcggtcgcgc gtgacaccgg
     3241 gctgatcgtc gaccagcgcg accttgcggc cgacgagccg attgaacagt gtggactttc
     3301 cgacgtttgg gcgcccgaga atggcaacgg tcagggtcat tgcggatcgg cgaacacgtc
     3361 gcccgatcct gcaccgcaat tcggctcctc cggcggacag ccagggtcgg accccacggt
     3421 cgaagcatcg gatggcgcgc ccacggagcc tccgccgctt gccgtctgcg cgcgctcgct
     3481 cggatccggg aactgcgcgc gcccgggcac ggcatcctcg cgcgcgccgg gcagcaccgt
     3541 attgtcggtc tcgcccgtca gccggtcgac atagctgcag cccgagagca tcaagcctgc
     3601 caaggccagc attgcgagtt tgcgcatcga tttcacttct gacccaagat cggcaagaga
     3661 aggtcggcga tcatgccggc acgctggcga agcggcgcgg gcgcttccgg atcgcccaga
     3721 atggcttgag cctggctgtc ggccccggcg tggtcgcccg ccttgaaggc cgagagcatg
     3781 aggatctcgc gggccatgtg gcgccacgga ttgccggccg tatcgaagtc cttcacccgt
     3841 gccgaaatgt cggcaaagct cgcactgtcg atcgacacat aggccgcacg cagccgggca
     3901 agatcgcgca ggggcgctgc cgttccggca ctcgctgcaa tggtatcgta aagctttatg
     3961 gcctcgtccg tcttgccgga ggccgcaagg acaccggcct cgcgcagctt cgccagctgg
     4021 gcatagcccg tatggccgat ggattcgaat tccttcagcg cctcttcgga ctttccggca
     4081 ttgccgttgg ccaccgcctg gaaaaagacc acgctggcgg cttccgcctg cgtcttctgc
     4141 caatactgcc agcccttgta gccggcgacg ccgagaacga tcagcaggca cagcgcaatg
     4201 aagatgttgc cgtattggct ccagatcttc ttgtactgct cctggcggac ttcctcgtcc
     4261 acctcgcgaa acagcgattc atcggtcaat tcggcatact ccccacggcg gatcagcgtg
     4321 agaagcgggc gtaaatcagc ggctgggcca aatcaaggca tttcggcggc ggtcgccgtc
     4381 gcgggcatag accgcccgga tggtcttcca gcgctcgtgg ctgatgtagg cataggccag
     4441 gccgccgacc atcagcgaac caaagaggaa accggctagt tccatcgcca tggggcacct
     4501 cgcagcagtc atcgactgaa atcgattcct gcaaattagg cgccccgatc cgtaaacatt
     4561 ccctaaagct ggtccggctt gaccacctgg aaagccccga tcagcccgcc gtcggagcgt
     4621 tccgccacca ggctctggaa ggcccaggtt cccggattgt cggccacgaa gcccagcagc
     4681 ttggtctggc cggccggcac caccgccgtg tcgctgaccg gcggctcgct gccctcctcg
     4741 gccgggagct gccagacatg gccatgcaga tgaagggcct ggctgaaggc cgtcctgttc
     4801 tcgatctcca gcgcaaggct ctcacccagc tgggcggcga agagcggttc gatccccgga
     4861 ccggccaccc cgttcagggc ccatccctta ccgttctcga gcagagtgcg gagttccatc
     4921 gtctggccgc gaaagacggc cgacttgaga ccgcccttga cgccgccctc gagggcaatc
     4981 ttcaccagcc gcggttcggg cttcgccggc gggcgggtga ccggattggg cggcagcgca
     5041 aaattgtcgt cgatcgctgc cggcgccgcc tcgccgtcgc gggtgaggta gccgatttcg
     5101 acgacatcct cgaagaggtc gagcgcgatg gtgatgtcgc cctcttccgc gctgaccaga
     5161 agatcggcgc gctgccccgg cgccagagtc agagccttgg tgcccagaag gcgcggcttc
     5221 accggctggc cgtcgaaggc gatcagcagc ggatcgctgc ccttgaactg cagtcccatg
     5281 ctgcgcacgt tggcggcgtt gagaatgcgc agccgggtga agcgtcccgc ggcaatgggc
     5341 aggcgcggcc ggaactggct gttgatggtc atccagttgc cgaggcggcc ctcgcccacc
     5401 attgtctcga tgtcgccgaa gccgccctcc atggttccgt cctgggcgat gcgccagtcg
     5461 tcgatgatca gcggcagatc ggggatcgcc ggatcgagcg cttccttctc ctcgaccacc
     5521 agcaccgcgt agagccccat gtcgcggtgg cgcgacacgt cggacacggg gccgatccag
     5581 aaggtgccgg catcgggcgg ggtgaacacg cagtccacac ccttgtcgcc gggcggcaca
     5641 ttgatggtca tcagttcgga tggaccgcgc aggccgaaga aatgcagcca gatctcgttg
     5701 tcgagttcgt tgaagatgcg cagtttcagc tcctggcctt gcttcgcgcg gatgaccgcg
     5761 ggcgccggac ccttgcccag aatccacgca ccggtcttgc cggcgccacc ttccagaagc
     5821 cccagttccg cattgcgggc gcgcagttcg atgaagtcat cggcgcgcgg cgcctggccc
     5881 ggcaccagca ccgccagact cgccgctgct gccaatatct gccgccgtgt aaatgacatc
     5941 tcgtgaacct tcggtgattg aagacatgtc tggtgcaggt cccgagaggc aagaagatgg
     6001 cagccgcacc acgcgccgca aactaactaa tctttcatgt cgtaggtgtt ctcgggctgc
     6061 gggaaggccc gcgacctcac gtcatcggca tacatcctga tggcaccttc gatggcagcc
     6121 ccgaccttgc cgaactcctt cacgaatttc ggcacgcgcg ggctgaggcc cagcatgtct
     6181 tccatcacca ggatctggcc atcgcaggcg gcggaagcgc cgatgccaat ggtcggaatg
     6241 ggtatctgcc tggtgatctt cgcggaaagc ggctcggcaa tggcctcgag caccacgctg
     6301 aaggcgccgg cctctgtcac cacccgcgca tcctcctcga tgagcgccca ttgctcgcgc
     6361 gtccggccct gggtgcggaa tccgcccagg atgttgatgg actgcggcgt gaggccgata
     6421 tgggccatca ccggaatgcc gcgctcggtg aggaagtgaa tggtctcggc catgcggcgt
     6481 ccgccttcaa gcttgatcgc gccgcattgc gtttccttga tgaccttcgc cgcattgtga
     6541 aaggcctgcg ag
//
LOCUS       DBNGVR010000438         2613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2715794, whole genome shotgun sequence.
ACCESSION   DBNGVR010000438 DBNGVR010000000
VERSION     DBNGVR010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2715794"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2610
                     /gene="carB"
                     /locus_tag="AC16A0_09360"
     CDS             <1..2610
                     /gene="carB"
                     /locus_tag="AC16A0_09360"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR3105584.1"
                     /translation="IEESVLGWKEYEMEVVRDKADNCIIICSIENVDPMGVHTGDSIT
                     VAPALTLTDKEYQIMRNASIAVLREIGIETGGSNVQFGVNPADGRLVVIEMNPRVSRS
                     SALASKATGFPIAKVAAKLAVGYTLDELANDITGGATPAAFEPTIDYVVTKIPRFAFE
                     KFPGAEPYLTTAMKSVGEVMAIGRSFQESMQKALRGLETGLTGFNEIAIAGAGEGDDK
                     NAIRAALGTPTPDRILKIAQAFRMGATVAQVFESCKYEPWFLRQIEEIVQTEAKIRAR
                     GLPKDAANLKRLKAMGFSDKRLGELVGLSEHKVRSHRHKLAVRPVYKRIDTCAAEFAS
                     PTAYMYSTYDAENEARPSDRQKVIILGGGPNRIGQGIEFDYCCCHACFALHDAGYESI
                     MVNCNPETVSTDYDTSDRLYFEPLTAEDVLEIIHTEQSNGTLKGVIVQFGGQTPLKLA
                     GALEKAGVPILGTTPDAIDLAEDRDRFSKLLRKLKLRQPENGIAYSGAQAKKIAKRIG
                     FPVVIRPSYVLGGRAMEIVKDDAQLERYITEAVVVSGDSPVLIDSYLSNAIEVDVDAI
                     CDGKDVFIAGVMEHIEEAGIHSGDSACSLPPYSLKPAVVAELERQAKVMALALNVVGL
                     MNVQFAIKDGDVYVLEVNPRASRTVPFVAKVIGLPVAKIAARVMAGESLASFKLKPKK
                     ISHVAVKEAVFPFNRFPGVDVLLGPEMRSTGEVMGLDKSYDMAFAKSQLGAGMRMPLV
                     GTVFISVKDADKARILPAMRRLVELGFKLVATGGTQRYFEEKGIPSTKINKVLEGRPH
                     VVDAIKNGDIQLVLNTTETRSSQSDSKPIRQTALLQKVPYYTTLPGILAVTKAIAAQK
                     AGTIEVKPLQDYF"
BASE COUNT          508 a          862 c          805 g          438 t
ORIGIN      
        1 atcgaggagt cggtgctggg ctggaaggag tatgagatgg aagtggtccg cgacaaggcg
       61 gacaactgca tcatcatctg ctcgatcgag aacgtcgatc cgatgggtgt gcacaccggc
      121 gactcgatca cggtggcccc ggcgctgacg ctcaccgaca aggaatacca gatcatgcgc
      181 aacgcctcga tcgcggtgct gcgcgagatc ggcatcgaga cgggcggctc caacgtgcag
      241 ttcggcgtca accccgccga cgggcgcctc gtcgtcatcg aaatgaaccc gcgcgtgtcg
      301 cgctcctccg cgctcgcctc caaggcaact ggcttcccca tcgccaaggt cgcggccaag
      361 ctggccgtgg gctacacgct ggacgaactg gccaacgaca tcacgggcgg cgccacgcct
      421 gcggccttcg agccgaccat cgactacgtc gtcaccaaga tcccgcgctt tgccttcgag
      481 aagttcccgg gcgcagagcc ttacctcacc accgcgatga agtcggtggg cgaggtcatg
      541 gccatcgggc ggtccttcca ggagtccatg cagaaggccc tgcgcggcct cgagacgggg
      601 ctcacgggct tcaacgagat cgccattgct ggcgccggcg aaggcgacga caagaacgcg
      661 atccgcgccg cgctgggcac gcccacccct gaccgcatcc tcaagatcgc ccaggccttc
      721 cgtatgggcg cgaccgtggc ccaggttttc gagtcctgca agtacgagcc ctggttcctg
      781 cgccagatcg aggagatcgt gcagaccgag gcgaagatcc gcgcgcgcgg cctgccgaag
      841 gacgcggcca atctcaagcg gctcaaggcc atgggctttt cggacaagcg gctgggcgaa
      901 ctcgtcggcc tctccgaaca caaggtccgc agccaccgcc acaagctcgc agtgcgcccc
      961 gtctacaagc ggatcgatac ctgcgcggcc gaattcgcct cgcccacagc ctacatgtac
     1021 tcgacctatg atgcggagaa cgaggcgcgg ccctcggacc ggcagaaggt catcatcctg
     1081 ggcggcggcc ccaatcgcat cggccagggc atcgagttcg actattgctg ctgccatgcc
     1141 tgctttgcgc tgcatgacgc gggctacgag tcgatcatgg tcaactgcaa ccccgagacc
     1201 gtgtcgaccg actacgacac ctcagatcgt ctctacttcg aaccgctgac cgccgaggac
     1261 gtactcgaaa tcatccatac cgaacagtcc aacggcacgc tgaagggcgt catcgtgcag
     1321 ttcggcggcc agacgccgct gaagctggcc ggcgccctgg aaaaggccgg cgtgcccatc
     1381 ctcggcacga cgcccgatgc catcgacctg gccgaggacc gcgaccgctt ctcaaagctc
     1441 ctcaggaaac tgaaactgcg ccagcccgag aacggcatcg cctattccgg cgcccaagcg
     1501 aagaagatcg ccaagcgcat cggcttcccc gtggtgatcc ggccttccta tgtgctgggc
     1561 ggccgggcca tggagatcgt caaggacgac gcccagctcg aacgctacat caccgaggcg
     1621 gtggtggtct cgggcgacag cccggtgctg atcgacagct atctctccaa tgccatcgag
     1681 gttgatgtcg acgcgatctg cgacggcaag gatgtcttca tcgccggcgt catggagcac
     1741 atcgaggagg ccggcattca ttccggcgac tcggcctgct cgctgccgcc ctattcgctg
     1801 aagcctgcgg tggtggccga gctggagcgc caggccaagg tcatggcgct cgcgctcaat
     1861 gtcgtcggcc tgatgaatgt ccagttcgcc atcaaggatg gcgatgtcta tgtccttgag
     1921 gtcaatccgc gggcctcgcg caccgtgccc ttcgtggcca aggtgatcgg cctgccggtg
     1981 gccaagatcg ctgcccgggt catggccgga gagtcgttgg ccagcttcaa gctgaaaccg
     2041 aagaaaatca gccatgttgc tgtaaaggaa gccgttttcc ccttcaatcg gtttcccggc
     2101 gtggacgtgc tgctcggtcc tgaaatgcgc tcgaccggag aggtgatggg gctggacaag
     2161 agttacgaca tggccttcgc caagagccag ctgggcgccg gaatgcgcat gccgctggtc
     2221 ggaaccgtgt tcatctctgt caaggatgcc gacaaggcgc gcatcctgcc ggccatgcgc
     2281 aggctcgtgg aactgggttt caagcttgtc gcaaccggag gcactcagcg ctatttcgag
     2341 gagaagggca ttccctcgac caagatcaac aaggtgcttg agggccgccc gcacgtggtg
     2401 gacgctatca agaacggcga catccaactc gtgctgaaca ccaccgagac gcgctcgtcg
     2461 cagtcggatt cgaagccgat ccgccagacc gcccttctgc agaaggttcc ctattacacc
     2521 accctgcccg gcatcctggc ggtgaccaag gccatcgcgg cgcaaaaggc cggcaccatc
     2581 gaggtcaagc cgctgcagga ctacttctga aca
//
LOCUS       DBNGVR010000439         2430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1145328, whole genome shotgun sequence.
ACCESSION   DBNGVR010000439 DBNGVR010000000
VERSION     DBNGVR010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1145328"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="AC16A0_09365"
     CDS             complement(<1..777)
                     /locus_tag="AC16A0_09365"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3105585.1"
                     /translation="MTSIVRFAPSPTGRIHIGNARTAILNWLMARKGGGQFVLRYDDT
                     DTARSTAAFADGIAADLAWLGILPDRVEWQSKRFAHYDEVANRLRKAGRLYACYETAD
                     ELDRRRKRQLARGLPPVYDRAALKLTDDDRARLAAEGRKPHWRFRLDGRVVKWDDLIR
                     GPQLIDTSTLSDPVLIREDGSYLYTLPSVIDDIDFGITHVIRGEDHVVNTAVQIEITE
                     ALGGAVPSYAHHSLLIGADGKGLSKRLGSLSIGAMREQGLE"
     gene            891..1880
                     /locus_tag="AC16A0_09370"
     CDS             891..1880
                     /locus_tag="AC16A0_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3105586.1"
                     /translation="MKQRRLGRNGPQVGEVGFGAMSFGGFFGPTDMGTSHRTLDQALE
                     LGVTHLDTAMIYGPHTSEEFIGAYFGKNPAAKKRFVIATKGGIAINPRGFDNSPAFLR
                     DCLEGSLRRLGVDHVALYYVHRRDHSLPIEEVTGVLADLVREGKIGAIGYSEISPASL
                     ERAAKVHPVAAVQSEYSLWSRQPELGMLQACARLGTSFVAFSPVARGMLTDVPLDPPS
                     FADIDFRKGNPRFTEPNFAWNVERIAAFKSFARQRGWTTAALAIAWTLRQGEHVIPIP
                     GTRSPDHLAENAAATDILLGEEDLREIDRILPPGFAHGHRYSEQQLKGVEQFC"
     gene            complement(1884..>2430)
                     /gene="nadE"
                     /locus_tag="AC16A0_09375"
     CDS             complement(1884..>2430)
                     /gene="nadE"
                     /locus_tag="AC16A0_09375"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR3105587.1"
                     /translation="EGTPSGITEENLQSRARGVILMAISNKFGAMVLTTGNKSEMSVG
                     YATLYGDMNGGYNPIKDVYKTAVYKLAAWHNTLGFVIPERIITKAPTAELRENQKDQD
                     SLPPYDVLDDILQGLVENELSMAEITARGHDEALVKRVQHMLYVAEYKRRQAAPGVKT
                     TRRNFGRDRRYPITNGFRDGR"
BASE COUNT          398 a          792 c          778 g          462 t
ORIGIN      
        1 ctccagtccc tgctcgcgca ttgcgccgat cgagagcgag cccagccgct tcgacaggcc
       61 cttgccgtcg gcaccgatca ggaggctgtg gtgggcatag gacggaacgg cgccgccaag
      121 ggcctcggtg atctcgatct gcaccgccgt attcacgacg tgatcttcgc cgcggatcac
      181 atgggtaatg ccgaagtcga tgtcgtcgat gaccgaggga agagtataga gatagctgcc
      241 gtcctcgcgg atcagtaccg gatccgagag cgtcgaggtg tcgatcagct gcggcccgcg
      301 gatgagatcg tcccatttca ccacgcggcc gtcgagcctg aagcgccagt ggggcttgcg
      361 tccttcggcc gccagcctgg cgcgatcgtc atcggtcagt ttcagggctg cgcggtcata
      421 gaccggcggc aggccacgcg ccagttgccg cttgcggcgc cggtcgagtt cgtcggccgt
      481 ttcgtagcag gcatagagcc gccctgcctt gcgcagccgg ttggccacct cgtcgtaatg
      541 cgcaaagcgc ttcgattgcc actccacgcg gtcgggcaga atgcccagcc aggccaggtc
      601 ggcggcgatg ccatccgcga aggcggcggt ggagcgggcc gtgtcggtat cgtcgtagcg
      661 cagcacgaat tgcccgccgc ctttcctggc catgagccag ttgagaatgg cggtgcgggc
      721 attgccgatg tgaattcgac cggtgggcga gggggcaaag cggacgatgc ttgtcatgcg
      781 ggcaagcgct aaccccaacc atccgcggcg tcaatgaggc agtccgctga ccggggcttg
      841 tgcgtgggcc cgcgtttgct attccagaga cctgtcaggg agaacacgca gtgaagcagc
      901 gccgcctggg ccgcaatggc ccgcaggtcg gggaagtggg gttcggggcc atgagcttcg
      961 gcggcttctt cggcccgacc gacatgggca ccagccatcg caccctcgat caggcactcg
     1021 agctgggtgt cacccatctc gacaccgcca tgatctacgg tccgcacaca tccgaggaat
     1081 tcatcggcgc ctatttcggc aagaaccctg cagcaaagaa gcgcttcgtg attgccacca
     1141 agggcggcat cgccatcaat ccgcgggggt tcgacaactc gcccgccttt ctgcgcgact
     1201 gcctcgaagg ctcgctcaga cggctcggcg tggatcacgt cgcactctat tacgttcacc
     1261 ggcgcgacca ttcccttccc atcgaggagg tcaccggcgt gctggccgat ctcgtgcgcg
     1321 agggcaagat tggcgcaatc ggctactccg agatctcgcc cgcgtcgctg gagcgtgccg
     1381 ccaaggttca tccggtcgct gccgtgcaga gcgaatactc gctgtggtcg cgtcagcccg
     1441 agctcggcat gctgcaagcc tgcgcccgcc tcggcaccag cttcgttgcc ttttcgcccg
     1501 tcgcccgcgg catgctcacc gacgttccgc tcgatccgcc atcctttgcg gacattgatt
     1561 tccggaaggg aaatccgcgc ttcacggaac ccaacttcgc ctggaacgtc gaacggatcg
     1621 cggccttcaa gtccttcgcg cggcagcggg gctggaccac ggctgcgctt gccatcgcct
     1681 ggaccctgcg tcagggcgag catgtcattc ccattcccgg cacgcggtca cccgaccatc
     1741 ttgccgagaa tgctgctgcc accgacatcc tgctcggcga agaggacctg cgcgagatcg
     1801 accgcatcct tccgcccgga ttcgcgcatg ggcaccgcta ttcggagcag cagctgaagg
     1861 gcgtggagca attctgctga gggtcagcgc ccgtcgcgga atccgttggt gatcggatag
     1921 cgccggtcgc ggccgaagtt gcgccgcgtg gtcttgacgc ccggtgcggc ctgccgccgc
     1981 ttgtattcgg caacatagag catgtgctga acccgtttca ccagcgcttc gtcgtggccc
     2041 cgcgccgtga tctcggccat cgacagctcg ttctcgacga ggccctgaag gatgtcgtcc
     2101 agaacgtcat agggcggaag cgaatcctgg tccttctgat tctcgcgcag ctctgcggtt
     2161 ggggccttgg tgatgatgcg ctctgggatg acgaagccca gggtgttgtg ccaggccgcc
     2221 agcttgtaga ccgccgtctt gtagacatcc ttgatcgggt tgtagccgcc attcatgtcg
     2281 ccatagagcg tggcatagcc caccgacatc tccgacttgt tgccggtggt cagcaccatg
     2341 gcaccgaact tgttggaaat cgccatcagg atcacgccgc gcgcgcgtga ctgcaggttc
     2401 tcctcggtga tgccgctggg cgttccttcg
//
LOCUS       DBNGVR010000440         5344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_162280, whole genome shotgun sequence.
ACCESSION   DBNGVR010000440 DBNGVR010000000
VERSION     DBNGVR010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_162280"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="AC16A0_09380"
     CDS             complement(<1..563)
                     /locus_tag="AC16A0_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HIR3105588.1"
                     /translation="MKPGLALAGLLLLAAAAHAAPVIVTAERLAAFQNLSPADRFGPF
                     VWRGGLVLQSDDPKFGGLSGLVLGKNCEDLTAISDAGRWFTAELSYDGQMLAGLTARD
                     LSPMLDSKGKPQRSKVWGDAEAVTEIAPGRLGVAYESRVRLGTYDVGKLGPKARFVHV
                     PYPGDIDAGDDNGEIEALGVVPVGPDKG"
     gene            complement(560..2437)
                     /gene="cobT"
                     /locus_tag="AC16A0_09385"
     CDS             complement(560..2437)
                     /gene="cobT"
                     /locus_tag="AC16A0_09385"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HIR3105589.1"
                     /translation="MAADRDSPAGEFKRVLTIAMKTIAEDPEVTVAFGSEQPSLAGNR
                     AKLPNVADTMKASDIAIVRGLSDSFALRIANHSEKVHAHYRPQGKNARAVFESVEQAR
                     IEAVGANAMPGMAANLTAMLDDRYRRKDVARYTDRKEAPLEDAVALIVREKLTGAPPP
                     DAAMPMVELWRDWIEDKASAQLAHLTDNIHDQAAFSRMSRDIIAALEMADELGDDPDQ
                     SDENDNEDEAEPDAGERQESPEGEDQESQQQSMDELQDSEGETEAAEMDAQQMDVDEQ
                     PDDQEGEEETEGEEPWRPQLPFSSLSNEDFYKVFTNQFDEEIDAEDLCDAEELTRLRN
                     YLDKQLSSLQGVVARLANRLQRRLMAQQNRSWDFDLEEGVLDAARLTRVVIDPMHPLS
                     FKVEQDTQFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTR
                     AWKGGQAREKWLAANKPANPGRLNDLRHIIYKSADMPWRRARRNLGLMMREGLLKENI
                     DGEALIWAHNRLLARQEQRRILMVISDGAPVDDSTLSVNSGNYLEKHLRQIITDIESR
                     SPVELIAIGIGHDVTRYYKRAVTIVDAEELGGAMTEKLAELFEEKEKAAPRAPGRRAR
                     A"
     gene            complement(2451..3407)
                     /gene="cobS"
                     /locus_tag="AC16A0_09390"
     CDS             complement(2451..3407)
                     /gene="cobS"
                     /locus_tag="AC16A0_09390"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HIR3105590.1"
                     /translation="MPDIKVSVKQLFGFDSNLEVPAFSQRTEHVPDVDEDYLFNRETT
                     LAILAGFAYNRRVMVTGYHGTGKSTHIEQVAARLNWPCIRINLDSHISRIDLIGKDAI
                     VVKDGKQVTEFKEGILPWAYQHNVALVFDEYDAGRPDVMFVIQRILESSGKLTLLDQS
                     KVIRPHPAFRLFATANTIGLGDTTGLYHGTQQINQAQMDRWSIISVLNYLPHAQETNI
                     VLSKNKAYNNEKGRKTIANMVRVADLTRNAFVQGDLSTVMSPRTVITWAENAEIFGDV
                     GFAFRVTFLNKCDELERSVVAEFYQRCFGEELPESSAHVAIA"
     gene            complement(3507..4115)
                     /locus_tag="AC16A0_09395"
     CDS             complement(3507..4115)
                     /locus_tag="AC16A0_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR3105591.1"
                     /translation="MKLDSKYFDKIRVKKPSEARKAAETAVPQCEWPDCPRPARHKAP
                     MGRGHEGKFHNYCTEHVQQYNKTYNYFDGMKDAELQDFQKDARLGHRPTWKLGRNGSA
                     NVTGMRRRAINMSQDPFGLNGVAAKEPGRHPTGRALRSTELKALQTLGLDDHATPEQV
                     KTQYKTLVKRLHPDANAGSRANEDTLKSVIQAYDHLRSSGFC"
     gene            4174..4440
                     /locus_tag="AC16A0_09400"
                     /pseudo
     CDS             4174..4440
                     /locus_tag="AC16A0_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493757.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
     gene            4423..4896
                     /gene="arsN2"
                     /locus_tag="AC16A0_09405"
     CDS             4423..4896
                     /gene="arsN2"
                     /locus_tag="AC16A0_09405"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HIR3105592.1"
                     /translation="MQAGPPAAPRADELTFSVIPSADPRLHRLLTAAQLPVHDLDAPT
                     LSFIGIVTPKDELVACGGLEMRGDAALIRSIAVAAGQRKSMLGTTITFKLLAEARNRG
                     AQAAYLLTATAQAFFSRLGFTAIERSKVPAPIAATSQFSGQACASANAMVSRLRS"
     gene            complement(4877..>5344)
                     /locus_tag="AC16A0_09410"
     CDS             complement(4877..>5344)
                     /locus_tag="AC16A0_09410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3105593.1"
                     /translation="ATSEGTKPDLKAFLAHAEALFGFSQARIRERRSGLIPCGGPVSP
                     FAAPGLLLIGDAAGHLSPLTGGGIRLAFRFGRRAGQLIADHLLRQGPPPDRVLAREMP
                     GFGKKLLLRRIMDFGPPNLLYDLAIGTPPMRWFARQVYFHRRQPEAPPSSGDG"
BASE COUNT          862 a         1667 c         1772 g         1043 t
ORIGIN      
        1 cctttgtctg gtccgaccgg caccacgccc agggcctcga tctcgccatt gtcatcgccg
       61 gcgtcgatgt cccccggata gggaacatgg acgaaacggg ccttcggtcc cagcttgccg
      121 acatcatagg tgccgagtct gacgcgactc tcataggcta cgcccagccg ccctggcgcg
      181 atttccgtga ccgcctcggc atcgccccag accttgctgc gctgtggctt gcccttgctg
      241 tcgagcatcg gagacagatc gcgcgccgtc aggcccgcaa gcatctggcc gtcataggac
      301 agttctgcgg tgaaccagcg gccggcgtcg ctgatggccg tgaggtcttc gcaattcttg
      361 cccagcacca gccccgacag cccgccgaat ttcgggtcgt cgctctgcag gacaagcccg
      421 ccgcgccaca cgaaggggcc gaagcgatcc gcggggctca gattctggaa ggccgccagc
      481 cgttcggcgg tcacgatgac gggggctgca tgcgcggcgg cggccagcag gaggaggccg
      541 gcaagtgcaa ggccgggctt cacgcgcggg cacgccggcc cggggcgcgg ggcgcggcct
      601 tttccttctc ctcgaagagc tcggccagct tctcggtcat tgcaccgccg agctcctcgg
      661 cgtcgacgat cgtcacggca cgcttgtagt agcgcgtcac gtcatggccg atgccgatgg
      721 cgatgagctc caccggcgag cggctctcga tgtcggtgat gatctggcgc agatgcttct
      781 cgagatagtt cccggagttt accgagagcg tcgaatcgtc gaccggcgcg ccgtcggaga
      841 tcaccatcag gatacggcgc tgctcctgcc gcgccagcag gcggttgtgc gcccagatca
      901 acgcttcgcc gtcgatgttc tccttgagaa ggccctcgcg catcatcagg ccgaggttgc
      961 gccgcgcccg gcgccacggc atgtccgcgg acttgtagat gatgtggcgg agatcgttga
     1021 ggcggccggg attggcgggc ttgttggcgg cgagccactt ctcgcgcgcc tgtccgccct
     1081 tccaggccct cgtggtgaag cccaggatct cgaccttcac gccgcagcgc tccagcgtgc
     1141 gggccaggat gtcggcgcag gttgcggcaa ccgtgatagg gcggccgcgc atcgaccccg
     1201 aattgtcgag cagcagcgtc accaccgtat cgcggaactg cgtatcctgc tccaccttga
     1261 aggagagcgg atgcatggga tcgatcacga cgcgggtgag gcgcgcggcg tccagcacgc
     1321 cttcctcgag atcgaaatcc caggaacgat tctgctgcgc catgaggcgg cgctgcagac
     1381 ggttggccag ccgcgccacc acgccctgca ggctggaaag ctgcttgtcg agatagttgc
     1441 gcagccgggt gagctcttcg gcgtcgcaga ggtcttcggc gtcgatctct tcgtcgaact
     1501 ggttggtgaa gaccttgtag aagtcttcat tggacagcga cgagaagggc aattgcggcc
     1561 gccagggctc ttcgccctcg gtctcttcct cgccctcctg gtcgtcgggc tgctcgtcga
     1621 cgtccatctg ctgggcgtcc atctcggcgg cctcggtctc gccctcggag tcctgcagct
     1681 catccatcga ctgctgctgg ctttcctggt cctcgccctc cggcgattcc tggcgctcgc
     1741 cggcatcggg ctcggcctcg tcctcgttgt cgttctcgtc cgactggtcg gggtcgtcgc
     1801 ccagctcgtc ggccatctcg agggccgcga tgatgtcgcg ggacatgcgc gaaaaggcgg
     1861 cctggtcgtg aatgttgtcg gtgagatggg ccagttgcgc gctggccttg tcctcgatcc
     1921 agtcgcgcca cagctcgacc atcggcatgg cagcgtccgg cggcggtgcg ccggtcagtt
     1981 tctcgcgcac gatgagcgca acggcgtctt ccagcggcgc ctccttgcgg tcggtgtagc
     2041 gcgccacatc cttccggcgg tagcggtcat ccagcattgc ggtgagattg gctgccatgc
     2101 cgggcatggc attggcgcca acggcctcga tacgggcttg ctcaaccgac tcgaagacgg
     2161 cgcgcgcatt cttgccctgg ggcctgtaat gcgcatgaac cttctcggag tggttggcaa
     2221 tgcgcagcgc gaagctgtcg gaaagtccgc gcacgatggc gatgtcggag gccttcatcg
     2281 tatctgccac attgggcagc ttggcgcggt ttccggcaag cgatggctgc tcgctaccga
     2341 aggccacggt cacttccggg tcctcggcga tggtcttcat cgcgatggtc aggacgcgct
     2401 tgaattctcc agccggtgaa tccctgtccg cggccatgtg ctaagggtcc tcaggcgatg
     2461 gcgacgtgcg cggaggactc cggcagttcc tcgccgaagc agcgctgata gaattcggcc
     2521 accacactgc gctcaagttc gtcgcacttg ttgaggaacg tcacgcggaa ggcgaatccg
     2581 acatcgccga agatctcggc attttctgcc caggtgatga cggtgcgcgg gctcatcacg
     2641 gtggacagat cgccctggac gaaggcattg cgggtgagat cggcgacgcg caccatgttg
     2701 gcgatggtct tgcggccctt ctcgttgttg taggccttgt tcttcgacag tacgatattg
     2761 gtttcctgcg catggggcag gtagttcagc accgagatga tggaccagcg gtccatctgc
     2821 gcctggttga tctgctgcgt gccgtgataa aggcccgtgg tgtcgccgag gccgatggta
     2881 ttggcggtgg cgaagagccg gaaggcagga tgcgggcgga tgaccttgga ctggtcgagc
     2941 agcgtcagct tgccggacga ctcgaggatg cgctggatca cgaacatcac gtcgggccgg
     3001 cctgcgtcat attcgtcgaa aacaagggcc acgttgtgct gataggccca ggggaggatg
     3061 ccctccttga actcggtcac ctgcttgccg tccttgacca cgatggcatc cttgccgatg
     3121 aggtcgatgc ggctgatatg gctgtccagg ttgatgcgga tgcagggcca gttgagacgc
     3181 gccgcaacct gttcgatgtg cgtggacttg cccgtgccat ggtagccggt caccatgacg
     3241 cgacggttgt aggcaaaacc ggccaggatc gcgagcgtgg tctctcgatt gaagagatag
     3301 tcctcgtcga catcggggac atgctcggtc cgctgcgaga aggccggcac ttcgagattt
     3361 gagtcgaacc cgaagagctg cttcaccgac accttgatgt caggcatgcc gttggcttca
     3421 ccgttcagct ttgcagtcat tggggcaggt cttctctatg agttttcggg gcgggtttta
     3481 gacggtcgcg ccccataggg caaggatcag cagaagccac ttgaacgcag gtgatcgtag
     3541 gcctggatca cggatttcaa ggtgtcttca ttggcgcggc tgccggcatt ggcatccggg
     3601 tgcaggcgct tcaccagcgt cttgtactgg gttttcacct gttcaggcgt ggcatgatcg
     3661 tcgaggccaa gggtttgcag cgctttcaac tctgtgctgc gcagggcgcg gcccgtgggg
     3721 tggcgtccag gctccttggc ggcgacgcca ttgaggccaa agggatcctg cgacatgttg
     3781 atggccctgc ggcgcatgcc ggtgacattg gcgctgccgt tgcgtcccag cttccaggtg
     3841 gggcgatggc cgaggcgggc atccttctgg aaatcctgaa gttcggcgtc tttcatgccg
     3901 tcgaagtaat tgtaggtctt gttgtactgc tgcacatgtt cggtgcagta gttgtggaac
     3961 ttgccctcgt ggccccgccc catcggcgcc ttgtggcggg ccggccgcgg gcaatcgggc
     4021 cactcgcact ggggcaccgc cgtttcagcc gccttcctgg cctcggaagg cttcttgacg
     4081 cggatcttgt cgaaatattt tgaatcgagt ttcatcgcgg cgcaattatg gggatgagcc
     4141 cgtcaacacg caaccccttc gcaaggtgac atcatgacac tcggtccggt cggacatcga
     4201 atctccacga agttggagca gactttcgcg ccagcctcgc tggaggtgat cgacgagtcg
     4261 caccagcatg cgggccatac cggtgcgcgg gcggatggcg agagccattt cagggtcagg
     4321 atcctggctg aggcgttccg cggcaagtca cgtgtggaac agcatcgcat gatcaacgcg
     4381 gttctggctg aagaactgcg cgaacgcgtg catgcgctgg cgatccaggc cgggccgcca
     4441 gcggcgccga gggccgacga gctgacgttc tcggtgatcc catccgcgga cccgcgcctg
     4501 catcgcttgc tgacggccgc acagcttccg gtccacgatc tcgacgcccc gacgctgagc
     4561 ttcatcggca tcgtgacgcc gaaggacgag cttgtggcct gcggaggact cgagatgcgc
     4621 ggcgacgcgg ccctcatccg ttcgattgcc gttgcggccg gccagcgcaa atccatgctc
     4681 ggcaccacca tcaccttcaa actgctcgcc gaagcccgca atcgaggtgc ccaggcggcc
     4741 tatctgctga ccgcaacggc tcaggctttc ttttcgcggc tgggcttcac cgccatcgaa
     4801 cgcagcaagg tgccggcgcc catcgccgcc acgagccagt ttagcggtca ggcctgtgcg
     4861 tctgccaacg ccatggtcag ccgtctccgg agctaggcgg cgcctccggc tggcgcctgt
     4921 gaaaatagac ctgacgggca aaccagcgca tcggcggcgt gccgatcgcg aggtcataga
     4981 gaaggttcgg tggcccaaag tccatgatgc ggcgcagcag cagtttcttg ccgaagcctg
     5041 gcatttcgcg tgccagcacg cggtcgggcg gcgggccctg gcgcagaaga tgatcggcga
     5101 tcagctggcc agcccggcgg ccgaaacgga aggcaagacg gattccgccc ccggtcagcg
     5161 gcgacaaatg cccagccgca tcgccgatca gcaagaggcc gggtgcagcg aagggcgaga
     5221 ccggtccccc gcatggaatg agcccgctgc ggcgttccct gatccgggcc tgcgagaatc
     5281 cgaagagtgc ctcggcatgg gcaaggaagg ccttgagatc gggcttggtt ccctcgcttg
     5341 tggc
//
LOCUS       DBNGVR010000441         1694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3502871, whole genome shotgun sequence.
ACCESSION   DBNGVR010000441 DBNGVR010000000
VERSION     DBNGVR010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3502871"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="ftsA"
                     /locus_tag="AC16A0_09415"
     CDS             <1..1071
                     /gene="ftsA"
                     /locus_tag="AC16A0_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3105594.1"
                     /translation="LAVDAAERMAQRNITEVFVNVSGGRPLSRLHSGAEKTQTGQVSP
                     RDLDRVLNAALAQVDPGRRAILHLAPVQYHLDDARGIQSPVGMYGETLALELNVVTVE
                     KAHLKNLTLAIESAHLGAAGFVVSSYAAAKSVLADDEMTLGATLVEMGGATTGFAIFH
                     DGHLVTSDIIPIGGMHITNDIARGLSTTIAHAERMKTLWGSALASQVDEREMIAVPLL
                     GERGVDTVQKVPKSMLTGIIRPRLEEIFELLRDKIEASGVAHLAGRRAVLTGGASQLT
                     GMRDIAAQWLDRQVRMGVPGHIQGMPESAHSPGFAVCAGLLHHALRPDTHYALPKAKA
                     EEIENAQQGYVRRMGRWIAESF"
     gene            1308..>1694
                     /locus_tag="AC16A0_09420"
     CDS             1308..>1694
                     /locus_tag="AC16A0_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3105595.1"
                     /translation="MTIKLTVPDLQDLKPRITVFGVGGAGGNAVNNMIESRLEGVEFV
                     VANTDAQALMQNAAQRRVQMGTALTQGLGAGSQPQIGAAAAEEALPEILDHLAGSHMA
                     FITAGMGGGTGTGAAPVIARAAKEQGI"
BASE COUNT          311 a          538 c          544 g          301 t
ORIGIN      
        1 cttgccgtcg acgccgccga gcgcatggcg cagcgcaaca tcaccgaggt cttcgtcaat
       61 gtctccggcg gtcgtccgct ctcgcggctc cattcgggag cagagaagac acagacaggg
      121 caggtgagcc cgcgtgatct tgaccgcgtc ttgaacgccg ccctggccca ggtcgatccc
      181 ggtcggcgcg cgattcttca tctggcgccg gtgcagtatc atctcgacga cgcgcggggc
      241 atccagtcgc cggtcggcat gtatggcgaa acgctcgctc tggaactcaa cgtcgtgacc
      301 gtcgagaagg cacatctcaa gaacctgacg cttgccatcg agagcgccca tctcggggct
      361 gccggcttcg tcgtgtcgtc ctatgcggcg gcaaagtccg tcctggcgga cgacgagatg
      421 accctgggcg ccacgctcgt cgaaatgggt ggcgccacga ctggcttcgc gatcttccac
      481 gatggtcacc tggtgacctc tgacatcatc ccgatcggcg gcatgcacat caccaacgac
      541 attgcacgcg ggctttccac caccattgcc catgccgagc gcatgaagac gctgtgggga
      601 tctgcccttg cttcgcaggt cgacgagcgc gagatgattg ctgtgccact gctcggcgaa
      661 cgcggggtgg acacggtgca gaaggtgccg aagtccatgc tcaccggtat catccggccg
      721 cggctcgagg aaatcttcga gctgctgcgc gacaagatcg aggcctcggg cgtcgctcat
      781 ctggctggcc gcagggcggt ccttaccggg ggcgcaagcc agcttacggg aatgcgcgac
      841 atcgcggccc agtggctgga caggcaggtc cgcatgggcg tgcccggcca tatccagggc
      901 atgccggagt cagcccattc gccgggcttt gcggtctgcg ccggactcct gcatcatgcc
      961 ctcaggccgg atacccacta tgccttgccc aaggccaagg ccgaggagat cgaaaacgcc
     1021 cagcagggct atgtacggcg catgggccgc tggatcgccg aaagcttctg agaacgcgtg
     1081 acattgcggc gggcctgaaa ccgggcacgc aggatgcggc ggcaacctcc gggatgccgc
     1141 cgtttccgtc aggtcagtcg gtgcttggta aatcggcggc taccctgccg caccggaatg
     1201 attcaatctg attcgaattt taggcatttg gttagcgcgc gttaaccatt tgttatggat
     1261 tgggtaagaa atatggagcg cggcaaatca atttccgggc gccaatcatg acaatcaagc
     1321 tgaccgttcc tgaccttcaa gatctgaagc cacgcatcac cgtgttcggg gtgggtggtg
     1381 ccggcggcaa tgccgtcaac aacatgatcg aaagccgtct ggaaggggtg gagttcgtgg
     1441 tcgcgaacac cgatgcccag gcgctcatgc agaacgccgc ccagcgccgc gtgcagatgg
     1501 gcaccgccct cacgcaggga ctcggcgccg gctcgcagcc gcagattggc gctgctgcag
     1561 cggaagaggc gcttcccgag atcctcgatc acctggccgg atcgcatatg gccttcatca
     1621 ccgccggcat gggcggtgga accggtaccg gcgccgcacc ggtcattgcc cgcgccgcca
     1681 aggagcaggg catc
//
LOCUS       DBNGVR010000442         1888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4484428, whole genome shotgun sequence.
ACCESSION   DBNGVR010000442 DBNGVR010000000
VERSION     DBNGVR010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4484428"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="AC16A0_09425"
     CDS             <1..785
                     /locus_tag="AC16A0_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3105596.1"
                     /translation="SDRAPAAAYLARGQAYRMKADYGDAIEDFDAVIDMDAAPALTAE
                     ALLGRGAAFAARGKLRKAIADLGRALDIGLPPGRQAAAYYYRGLAHADAGDFDQSVAD
                     FDVAIRLGFTSARAYGYRGSANAARGDYDLAIRDYDAAIRLDRRGAHYFAGRCWAMGK
                     TEQFDRALADCTRAIELDAGLSAGYEGRALALESTGSVAAAEKDYRRALKLDPTSQTA
                     RDGLKRLASVEAEPASTSKSVKKVAVPGQAGTGELIRRNRKP"
     gene            complement(760..1719)
                     /locus_tag="AC16A0_09430"
     CDS             complement(760..1719)
                     /locus_tag="AC16A0_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HIR3105597.1"
                     /translation="MWVIDSHTEGEPTRLIIDGRPRLTGRTLAEKRDHFAHEFDHIRR
                     FAVLEPRGHDALVGALLCEPENSSSAAGVIFFNNAGCLGMCGHAAIGTIISLAHLGRI
                     GHGIHRLDTPVGPVTLALRAGNEVTVENVPSHVHRSGVALDVPGIGRIAGDIAWGGNW
                     FFITDDLGLELTLARVRALTDATLRVRDALAAAGHKGPHGEEIDHIELTGPSSAADSR
                     NFVLCPGGACDRSPCGTGTSAKLACLAAAGKLAPGEGWIQESITGSRFSATYRAADRG
                     IIVSITGRAFVTADARLITSPGDPFADGFADAPQPVRACGFAG"
BASE COUNT          315 a          600 c          643 g          330 t
ORIGIN      
        1 cttccgatcg cgcgccggcg gccgcctatc tggcccgcgg ccaggcctat cgcatgaagg
       61 ctgactacgg ggatgcgatc gaggatttcg acgctgtcat cgacatggac gccgcgccgg
      121 cgctgacggc cgaggcgctg ctcggacgcg gtgccgcctt cgccgcgcgg gggaagctca
      181 gaaaggccat cgccgatctc ggccgcgcgc tggacattgg cctgccgccg ggccggcagg
      241 ccgccgccta ctactaccgc ggtctcgctc atgctgatgc cggggatttc gatcagtcgg
      301 tggcggactt cgatgttgcc atccggcttg gtttcaccag cgcccgggcc tacggctatc
      361 gcggctcggc caatgccgcg cgcggtgact atgacctggc gatcagggac tatgacgccg
      421 caatccggct tgaccggcgc ggggcgcatt atttcgcggg gcggtgctgg gcgatgggca
      481 agaccgaaca gttcgaccgc gcgctggcgg attgcactcg tgccatcgaa ctcgatgccg
      541 ggctttctgc cggctacgag gggcgcgccc tggccttgga gagtaccggc agcgtcgcgg
      601 cggcggagaa ggactatcgc cgtgcgctga agctcgaccc cacgtcgcag accgcgcgcg
      661 acggcttgaa acgccttgct tcggtcgagg ccgagccggc gtcgacatcg aagtcggtca
      721 agaaggttgc cgtgcccggc caagccggga ccggtgagct tatccggcga aaccgcaagc
      781 cctgacgggc tgtggtgcat cggcaaatcc atcggcgaaa ggatctccgg gagaggtgat
      841 cagccttgca tcggcggtca cgaaggcgcg gcccgtgatg ctgacaatga ttcccctgtc
      901 ggccgcacga taggtcgcgg aaaatcggct gccggtgatg ctttcctgga tccacccttc
      961 gccgggcgcg agttttcccg cagctgcgag gcaggccagc tttgcgctgg tgccggttcc
     1021 gcaaggcgaa cggtcacagg cgccgccggg gcagaggacg aagttgcgcg aatctgcggc
     1081 ggatgagggg cccgtcagct cgatgtggtc gatctcttcg ccatgaggcc ctttgtggcc
     1141 tgctgcggcg agcgcatctc tgacgcgcag cgtggcgtcc gtaagtgcgc ggaccctcgc
     1201 cagcgtcagc tccagcccca gatcgtccgt gatgaagaac cagttcccgc cccaggcgat
     1261 gtcaccggca atgcgcccga tgccgggcac atcgagcgcg acaccgctgc gatgcacatg
     1321 gctcggcacg ttctcgacgg ttacttcgtt gcctgcacga agcgcgaggg tcaccggtcc
     1381 caccggcgta tccaggcgat ggatgccatg tccgatgcgc cccagatgcg ccaggctgat
     1441 gatggtaccg atggccgcgt ggccgcacat tccgaggcag ccggcattgt tgaagaagat
     1501 cacgcctgcg gcggaagagc tgttttcggg ctcgcagagc agggctccca cgagagcatc
     1561 gtgaccgcgc ggttcaagca ccgcaaaacg gcggatatgg tcgaactcat gtgcaaagtg
     1621 atcacgcttt tctgcgaggg tgcggccggt caatcgcggc ctgccgtcga taatgagacg
     1681 cgtgggctca ccctcggtgt gagagtcgat cacccacatg tcagcgcgtc cgtccgggcc
     1741 aggtggcata ccaggccttg aacagggcaa gctgtctgtc ggcataggcc ctctggctct
     1801 cggacagaac atccgattcg atgatctggc gcgcataggc tgcctcgcct tcacggatca
     1861 tgaggtgctt gtagtagagg accagatc
//
LOCUS       DBNGVR010000443         1986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3307574, whole genome shotgun sequence.
ACCESSION   DBNGVR010000443 DBNGVR010000000
VERSION     DBNGVR010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3307574"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..147
                     /locus_tag="AC16A0_09435"
     CDS             10..147
                     /locus_tag="AC16A0_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016370.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HIR3105598.1"
                     /translation="MEQLGYRVLRFGNEEVLNGMDGVLSLISGALSEAPLAARPIAGP
                     P"
     gene            200..367
                     /gene="rpmG"
                     /locus_tag="AC16A0_09440"
     CDS             200..367
                     /gene="rpmG"
                     /locus_tag="AC16A0_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011179398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR3105599.1"
                     /translation="MAKGNVIKIKLASTADTGYFYVTKKNSRTMTEKMSVKKYDPVAR
                     KHVEFKETKIK"
     gene            469..1953
                     /locus_tag="AC16A0_09445"
     CDS             469..1953
                     /locus_tag="AC16A0_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR3105600.1"
                     /translation="MSTPDYRAMIPGLVYRNQAFINGRHVPAASGRTFDCVSPIDGRV
                     LTQVAECDAEDVNRAVAAARAAFEKGGWSACHPQKRKKVLTRFAELILKHRDELALLE
                     TLDMGKPIASSAGGDIPASAQAIQWYGEAADKQYDDVAPYRPDSVALITREPIGVVAA
                     VVPWNYPLMMASWKLGPALAAGNSIILKPAEQSPLTALRIAELAAEAGVPDGVLNVLP
                     GFGETAGQALGRHMDVDMIAFTGSTQVGKYFLRYSGESNMKHVSLETGGKSPNIVFAD
                     AGDLDHVARTSADGVFYNSGQSCNAPTRILVEDSIREEFMALVAKHARPWTPSDPLDP
                     KTAMGAVVDRDQMNRVLSYIDAGKAEGARLAAGGHQALSETGGSYIAPTVFEAVTNTM
                     KIAQEEIFGPVVVAISFKDEAEAIRIANDSIYGLQANIWTRDVSRVHRVARQLRAGTV
                     VVNNVWGSDISHPFGGYKQSGIGRDKSLHAMDKYQQVKNTYIQL"
BASE COUNT          383 a          668 c          611 g          324 t
ORIGIN      
        1 acggcctttt tggagcagct gggatatcgc gtcttgcgct ttggaaatga agaggtcctg
       61 aatgggatgg atggcgttct ctccctgatt tccggagccc tgagtgaggc ccccctcgcc
      121 gcccgcccga ttgccgggcc gccttgactt tgggacgctt ttcgggtcta tcggcgccaa
      181 atcccgattg gagagcatca tggccaaagg caatgtcatc aagatcaagt tggcgtccac
      241 cgccgacacc ggttacttct acgtgaccaa gaagaattcc cgcaccatga ccgagaagat
      301 gtcggtcaag aagtacgacc ccgtcgcgcg caagcacgtc gagttcaagg aaaccaagat
      361 caagtagtcc aatcgccttc gggccgggac aaggggcacc gccacgagcg gtgcctttcg
      421 cgtttcaggg ccctgccgcg tgaaatcccc tcacctctgg agctgcccat gagcaccccc
      481 gactatcgcg ccatgatccc cggcctggtc taccgcaacc aggccttcat caacggccgg
      541 cacgtgccgg cggcctcggg caggaccttc gactgcgtct cgccgatcga tggccgtgtg
      601 ctgacccagg tcgcggaatg cgatgcggag gacgtgaacc gcgccgttgc ggcggcccgc
      661 gcggccttcg aaaagggcgg ctggagcgcc tgccatccgc agaagcgcaa gaaggtgctg
      721 acgcgcttcg ccgaactcat cctcaagcac cgcgacgaac tggccctgct cgaaaccctc
      781 gacatgggaa agcccatcgc ctcctcggca ggcggcgaca ttccggcctc cgcgcaggcc
      841 atccagtggt acggcgaagc agccgacaag cagtatgacg acgtcgcgcc ctatcgcccg
      901 gactcggtgg ccctgatcac ccgcgagccc atcggcgtgg tggcggccgt ggtgccgtgg
      961 aactatcccc tgatgatggc atcctggaag ctcggccctg ccctcgccgc gggcaattcc
     1021 atcatcctga agcccgccga gcaatcgccg ctcaccgcgc tccgcatcgc cgaactggcc
     1081 gccgaagccg gagtgcccga cggcgtcctg aatgtgcttc ccggcttcgg cgaaacggca
     1141 ggacaggccc tcggccgcca catggacgtg gacatgatcg ccttcaccgg ctccacccag
     1201 gtcggcaagt atttcctgcg ctattccggc gagagcaaca tgaagcatgt ctcgctcgag
     1261 acgggaggca agtcgcccaa catcgtcttc gccgatgccg gcgatctcga ccacgtggca
     1321 cgcacctccg ccgacggcgt cttctacaat tcggggcaga gctgcaacgc gcccacccgc
     1381 atcctggtgg aggacagcat ccgcgaagag ttcatggcgc tggtcgccaa gcacgccagg
     1441 ccatggactc cttcggatcc gctcgacccg aagaccgcca tgggcgccgt ggtcgaccgc
     1501 gaccagatga accgcgtgct gtcctatatc gacgcgggca aggccgaagg tgccaggctc
     1561 gcggcgggcg ggcatcaggc gctgagcgag acgggcggca gctacatcgc gccaacggtc
     1621 ttcgaggccg tgaccaacac catgaagatc gcccaggaag agatctttgg cccggtcgtc
     1681 gtggccatca gcttcaagga cgaggcagag gccatccgca tcgccaatga ctcgatctat
     1741 gggctgcagg ccaatatctg gacccgcgac gtgagccggg tgcaccgggt ggcgcgccag
     1801 ctgagggccg gcacagtggt ggtcaacaat gtctggggca gcgacatcag ccatcccttc
     1861 ggcggctaca agcagtccgg catcggccgc gacaagtcgc tccatgccat ggacaagtat
     1921 cagcaggtca agaacaccta cattcagctc taggtgatag aggggcaccg gctgcgcagg
     1981 actctc
//
LOCUS       DBNGVR010000444         2926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4681657, whole genome shotgun sequence.
ACCESSION   DBNGVR010000444 DBNGVR010000000
VERSION     DBNGVR010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4681657"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1553)
                     /locus_tag="AC16A0_09450"
     CDS             complement(156..1553)
                     /locus_tag="AC16A0_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3105601.1"
                     /translation="MTDLHPILSAAEKDFDQSLERLFALCRIPSISTDPAHAADCDRA
                     AAAIVADLSSMGFDAKARPTPGRPMVVAHYTPKDANAKTPHVLFYGHYDVQPADPLEL
                     WKSPPFAPQRRKDADGVERLYGRGTADDKGQFMTFVEAARAWITATGSLPIKATFLIE
                     GEEESGSPSLVPFLKANRKELSCAVAFVCDTNMWDAKTPAITTRLRGLVHEEITIIGP
                     RIDLHSGMYGGPAINPIRVLSKVIAALHDKTGKITIPGFYDGVAPLPAATKKQWKALK
                     FSDAKFLKEVGLTVPAGEKGKSVLEQIWARPTAEVNGVWGGYTGAGTKTVLPSQAHAK
                     FTFRLVGKQDPEKVLKAFQAFVKARIPKDCKVTFSGRGGGSPATEIPESNPWISKSAA
                     ALKAEFKRDAVLMGSGGSIQICRSFKDILGMDSVLVGFGLNDDAIHSPNEKYNLASFQ
                     RGIRSWIRIIGGLAS"
     gene            complement(1564..>2926)
                     /locus_tag="AC16A0_09455"
     CDS             complement(1564..>2926)
                     /locus_tag="AC16A0_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3105602.1"
                     /translation="DQQFTFSLDRLFEFLRFPSVGTDPQYHGSCRDAARWLEGILSAM
                     GFEASIRETTGQPVVIGKLDPPAASLRLPHILFYGHYDVQPADPLQLWDSPPFSPQIR
                     RGKDGKDRIFARGASDDKGQLMTFLEASRAWLSQVGSLPFRLTIMIEGDEEGDSTHID
                     RFLALNAREFKADAAFICDTELWMDETPAIITRLRGCIDEEVIVTGPKIDLHSGYYGG
                     PAVNPIKVLSRILADMHDSRGRVTIPGFYEGVKPPSAERRREWSKLRFSAKKYLGDVG
                     LSRPAGEAAFTPLEQMWARPTAEFNGIFGGYRGAGSKTVLPAEATAKLTFRLVEGQNP
                     RKIRKAFRDFVKARLPKDCKARFSGGHGDSTGISIATDTPWVIAARKALNDEWGRPAA
                     LIGCGASIPVVESFKTHLGIDSLLVGFARDADSVHSPNEKYDVECYRKGIRSWARIIS
                     EFA"
BASE COUNT          531 a          904 c          940 g          551 t
ORIGIN      
        1 catcgactgc aggatggctt cgcgtcctgc gagcacgcca atggcgggcg agtgataggt
       61 ggcatcgggc gcgatcatgg ggttcaccag attcacgtca aatgcgttca acacctcgtg
      121 gtattgcctc acgatagcca gcgcgtcgat cgcattcacg atgccaggcc gccgataatg
      181 cggatccagc ttcggatgcc gcgctggaag cttgcaagat tgtatttctc gttgggactg
      241 tggatcgcgt cgtcgttcag gccgaacccg acaagtacgc tgtccatgcc gaggatatcc
      301 ttgaaggagc ggcagatctg aatcgaaccg cccgatccca tcagcacggc atcgcgcttg
      361 aactcagcct tgagggcagc ggccgatttc gagatccatg gattgctctc cgggatctcg
      421 gtggccgggc tgccgccgcc gcgcccggag aacgtgacct tgcagtcctt cgggatacgc
      481 gccttcacaa acgcctggaa ggccttcagc accttctcag gatcctgctt gccgaccagc
      541 cggaaggtga acttggcatg ggcctgcgac ggcaggaccg tcttggtgcc ggcgccagta
      601 tagccgcccc agacgccatt cacctccgcc gtgggccgcg cccagatctg ctcaagcacg
      661 ctcttgccct tctcgcccgc cggaaccgtg aggccgacct ccttcagaaa cttcgcgtcc
      721 gagaacttca gggccttcca ctgcttcttc gtggcggccg gcagcggcgc cacgccgtca
      781 tagaagccgg gaatggtgat tttgcccgtc ttgtcatgca gggctgcgat caccttgctc
      841 aggacgcgga tgggattgat cgcaggtcca ccatacatgc cggaatgcag atcgatgcgc
      901 ggcccgatga tggtgatctc ctcatgcaca agtccgcgca ggcgcgtggt gatggccggc
      961 gtcttcgcat cccacatgtt ggtgtcgcag acgaaggcca cggcgcagga cagttccttc
     1021 ctgttggctt tcaaaaaggg cacgagcgac ggcgaacccg attcttcctc gccttcgatg
     1081 aggaaggtcg ccttgattgg cagcgagccg gtcgccgtga tccaggcgcg tgccgcctcg
     1141 acgaaggtca tgaactgtcc cttgtcgtcc gcagtgccgc ggccatacag gcgttcgacg
     1201 ccatccgcat ccttgcggcg ctgcggcgcg aaaggcggag acttccaaag ctcgagcgga
     1261 tccgcaggct gtacatcata atgcccgtag aacaggacat gcggcgtctt cgcattcgca
     1321 tccttgggcg tgtagtgcgc cacgaccatc gggcgcccag gtgtcggcct ggccttcgca
     1381 tcgaaaccca tggacgaaag gtcggcgacg atggccgcgg cggccctgtc gcagtcggcg
     1441 gcatgggcgg gatcggtcga gatcgagggg attcggcaga gggcgaagag ccgttcaagg
     1501 ctctggtcga aatccttctc ggcggcggag aggatgggat gaaggtcggt catggatcgt
     1561 tcctcaggca aattcggaga tgatgcgggc ccagcttcgg atgcccttgc gatagcattc
     1621 gacgtcgtat ttctcgttgg gactgtgcac gctgtcggcg tcgcgtgcga agccgaccag
     1681 caggctgtcg attcccagat gggtcttgaa ggactcgacc acggggatcg atgcgccgca
     1741 gccgatcagc gcggcgggcc tcccccattc gtcgttcagg gccttccgcg cggcaatgac
     1801 ccagggcgtg tccgtcgcaa tcgagatgcc ggtactatcg ccgtgaccgc cggagaacct
     1861 cgccttgcaa tccttcggca ggcgggcctt cacgaagtcc cggaatgcct tgcggatctt
     1921 gcgtgggttc tgaccttcca cgaggcggaa ggtgagtttg gcggtggctt ccgccggcaa
     1981 gacggtcttg gaaccagcgc cgcgataacc gccgaagatg ccgttgaatt ctgcggtcgg
     2041 gcgggcccac atctgttcga gcggcgtgaa tgccgcctcg ccagcaggcc gcgacaggcc
     2101 gacatcgccg agatacttct tggccgaaaa ccgcagcttc gaccattccc gccggcgctc
     2161 ggccgagggg ggcttcaccc cttcgtagaa gcccggaatt gtcacccggc cccgggagtc
     2221 gtgcatgtcg gccaggatgc ggctcagcac cttgatcgga ttgacggcgg ggccgccata
     2281 atagccggag tgaaggtcga tcttcggccc ggtgacgatc acctcctcat cgatgcagcc
     2341 acgcagccgc gtaatgatgg ccggggtttc gtccatccag agttccgtat cgcagatgaa
     2401 cgcggcatcc gccttgaact cgcgcgcatt gagcgccagg aagcggtcga tgtgggtgga
     2461 atccccctcc tcgtcgccct cgatcatgat cgtcagccgg aagggcaagg agcccacttg
     2521 cgagagccat gcccggctgg cttcgagaaa ggtcatcaac tggcccttgt cgtcgctggc
     2581 ccccctggca aagatgcgat ccttgccatc ctttcccctg cggatctgcg gcgagaaggg
     2641 cgggctgtcc cacagctgca gcggatcggc gggctgtaca tcgtaatggc catagaagag
     2701 gatatggggc agccgcagcg aggcggccgg agggtcaagt tttccgatca cgaccggctg
     2761 gccggtggtt tcccggatcg aggcctcgaa acccatggcc gacaggatcc cctccagcca
     2821 gcgagcggca tcccggcaag acccgtggta ttgcggatcg gtaccgacgg agggaaagcg
     2881 gagaaattcg aacaggcggt cgaggctgaa agtaaattgt tgatca
//
LOCUS       DBNGVR010000445         3028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1147960, whole genome shotgun sequence.
ACCESSION   DBNGVR010000445 DBNGVR010000000
VERSION     DBNGVR010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1147960"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="AC16A0_09460"
     CDS             complement(<1..924)
                     /locus_tag="AC16A0_09460"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3105603.1"
                     /translation="MTDYENFIKTPGRADLIKQVRKKIDALGIEYLYLQFVSVTGRIM
                     GKGIPADHWENIANGGFQLVYGSTVNLFMNRRGEYLGYGPEAAELVGVPEPETFMQLP
                     WDKRVARMFCTLFRNREEREDPGAFLTADCRGNLRRIHDQFEKDHGLRMRHGTEPEMM
                     WLKKDENGKPNGGFSNPYCYHIDQFESLRPVYMKVIEYGRKMGLDMIQGDHEDAPGQI
                     ELNFNYDDALRTADRLTTYRQICAQVAREFNIIACFMCKPFMGVSANGCHHNISLWKG
                     GKDEFKALGNDPKNLPGFEHNFMYRRGGTNTF"
     gene            complement(1023..2369)
                     /locus_tag="AC16A0_09465"
     CDS             complement(1023..2369)
                     /locus_tag="AC16A0_09465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3105604.1"
                     /translation="MAVPSGVKYAIIGAGIHGLSTAYHLALKLRETGRGSGKDILVVD
                     KTAIAAGASGIACGVVRNNYYQPAMRELMAQCVEVWESDPKAYSYHSVGYMQISPEVM
                     HVQVGSIYEQQKKIGYDSVFIEGAGDSQKYMEGLFSDWQAKGITSVLHEKKGGYANNT
                     ASIYGLASKAEAEGVRILTGCEVTGFEFGNNSAAVTGIKTNKGTIKCDYVVVGVGPWV
                     NTIWNMLELPRTVSIKGRDGKTHDGIRMWKYWCLEEGTLGVDPNMHKTNDGRMPPVIH
                     VDTDAPLYSDVDGRLLTDKLWGLYYKPDFNFGGIQGGSSPYKVEGEPDTIAIDPYGPD
                     SPDFIVGDDFIETWCSMLAHCQKRFEGKISQYKNHEKSGGLGCFTPDNFPVFDVFREN
                     VFVIADSNHGYKMIGVGKLVAQEICGETSRLLEPFRFSRYAEGKLHPVSNSPFPWS"
     gene            2616..>3028
                     /locus_tag="AC16A0_09470"
     CDS             2616..>3028
                     /locus_tag="AC16A0_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3105605.1"
                     /translation="MARIKQAIESGVYADGDQLPAERQLAITFGTARSTIRKVLDQLA
                     EKNLVVRRVGSGTFVSYAGPIHQGMEDIADLVSPLQLIETRFAIEPYMTRLAAIHATG
                     SDLDTFEDVLRRIEAAEDDPTLFTQLDSEFHLDLAR"
BASE COUNT          561 a          897 c          927 g          643 t
ORIGIN      
        1 gaaggtgttg gtgccgccgc ggcggtacat gaagttgtgc tcgaagccgg gcaggttctt
       61 cgggtcgttg ccgagcgcct tgaactcgtc cttgccgccc ttccacagcg agatgttgtg
      121 atggcagccg ttggccgaca cgcccatgaa gggcttgcac atgaaacagg cgatgatgtt
      181 gaactcgcgg gccacctgcg cgcagatctg gcgataggtg gtcaggcggt ctgcggtgcg
      241 cagcgcatcg tcgtagttga agttgagctc gatctgtccg ggcgcatcct cgtgatcgcc
      301 ctggatcatg tcgaggccca tcttgcggcc gtattcgatg actttcatgt agaccgggcg
      361 gaggctctcg aactgatcga tgtgatagca gtaggggttc gagaatccac cattcggctt
      421 gccgttctcg tccttcttca gccacatcat ttccggctcg gtgccgtgac gcatgcgcag
      481 gccgtggtcc ttctcgaact ggtcgtggat gcggcgcagg tttccgcggc aatcggcggt
      541 gaggaaggca ccgggatctt cacgctcctc gcggttgcgg aagagggtgc agaacatgcg
      601 cgccacgcgc ttgtcccagg gcagctgcat gaaagtttcc ggttcgggaa cgccgacgag
      661 ctcggcggcc tccgggccgt agccaagata ctcgccgcgg cggttcatga agaggttgac
      721 ggtggagccg taaacgagct ggaagccgcc attggcgatg ttctcccagt ggtcagcagg
      781 aatgcccttg cccatgatgc ggccggtgac cgacacgaac tggaggtaga ggtactcgat
      841 gccgagcgcg tcgatcttct tgcgcacctg cttgatgaga tcagcgcgcc ccggagtctt
      901 gatgaagttc tcgtagtcgg tcatcggcat ttccttccct gtttttcttg ctggggtctc
      961 ttgccccgtg aactgaacag ccaatccaag tcccgcttgt ccgcggtgtc aaatctctcc
     1021 ggtcagctcc aggggaacgg actgttggac acgggatgca gcttgccttc cgcatagcgc
     1081 gagaaacgga agggctcgag caggcgagaa gtctcgccgc agatctcctg ggcaaccagc
     1141 ttgccgacgc caatcatctt gtagccgtgg ttcgagtcgg cgatgacgaa gacattctcg
     1201 cggaacacgt cgaagaccgg gaagttgtcg ggcgtgaagc agccgaggcc gccggacttc
     1261 tcgtgattct tgtactggct gatcttgcct tcgaagcgct tctggcaatg ggccagcatc
     1321 gagcaccagg tctcgatgaa gtcgtcgccg acgatgaagt cgggcgagtc aggcccgtag
     1381 ggatcaatcg cgatggtgtc aggctcgcct tcgaccttgt agggcgacga accgccctgg
     1441 atgccgccaa agttgaagtc cggcttgtag taaagacccc agagcttgtc ggtgagaagg
     1501 cggccatcga cgtcggagta gagcggcgca tcggtatcga catggatcac cggcggcatc
     1561 ctgccgtcat tggtcttgtg catgttggga tcaacgccca atgtgccctc ttcaaggcac
     1621 cagtatttcc acatgcggat gccgtcatga gtcttgccat cgcgtccctt tatcgagacc
     1681 gtgcgcggca gttccagcat gttccagatc gtattcaccc agggaccgac gccgaccacg
     1741 acgtaatcgc acttgatcgt gcccttgttc gtcttgattc ccgtgactgc cgcggagttg
     1801 ttgccgaatt cgaagcccgt gacctcgcag ccggtcagga tgcgaactcc ttcagcctct
     1861 gccttggagg cgagaccata gatcgaggcc gtattgttgg cgtagccgcc cttcttttcg
     1921 tgcaggaccg aggttatgcc cttcgcctgc cagtcagaga agaggccctc catgtatttc
     1981 tgggagtcgc ctgctccttc gatgaagacc gagtcgtagc cgatcttctt ctgctgctcg
     2041 tagatcgaac ccacctgtac gtgcatgacc tccgggctga tctgcatgta gccgacgctg
     2101 tgataggaat aggccttggg atcgctctcc cagacctcga cgcattgggc catcagctcg
     2161 cgcatggccg gctggtagta gttgttgcgg acgacaccgc aggcgatgcc ggacgcgccg
     2221 gccgcgatcg cggtcttgtc caccacgagg atgtccttgc cgctgccgcg gccggtttcc
     2281 ctgagcttca gtgcgagatg ataggctgtc gagagaccgt ggatgcctgc gccgatgatg
     2341 gcgtacttga cgccggacgg aactgccatg ggccgaattc ctcttgttgc tgaccggttc
     2401 cgctcaaaca cgggtaaacg cagcttgacg cggagccaga accgggcctt attgatccac
     2461 aaaagccgac cgattgcaaa ccaattctgt ccaattacaa ccaatctccc cttcattggt
     2521 tgcgtccgtc cggcaaaatg gggaacagat tgacggaagc gacagacgac tcagatgacc
     2581 tgctggccgg ttcgagccgt tccgcaagcg tcatcatggc gcgcatcaag caggcaatcg
     2641 agtccggcgt ttatgccgat ggcgatcagt tacccgccga gcggcagctt gccatcacct
     2701 tcggcacggc gcgttcgaca attcgcaagg tgctggacca gcttgccgaa aagaacctcg
     2761 tcgtgcggcg ggttgggagc ggcaccttcg tgagctacgc cggacctatc caccagggga
     2821 tggaagacat tgcggacctg gtcagccctc tgcaattgat cgaaacccgc tttgccatcg
     2881 aaccctacat gacccggctg gcagcaatcc atgcgaccgg cagcgatctc gataccttcg
     2941 aggacgtcct gcgccggatc gaggcggcgg aggacgatcc gaccctgttc acccagctcg
     3001 attccgagtt tcacctcgac ctcgcgcg
//
LOCUS       DBNGVR010000446         2797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3110695, whole genome shotgun sequence.
ACCESSION   DBNGVR010000446 DBNGVR010000000
VERSION     DBNGVR010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3110695"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="AC16A0_09475"
     CDS             complement(<1..922)
                     /locus_tag="AC16A0_09475"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3105606.1"
                     /translation="MSADIVIENAAILTMDDARPKAEALAITGNRIAAVGSNREIAAE
                     KGASTRVIDAGGATVTPGLVEAHMHLFAGAAELKHLQLAGTQGFDELKSRTLAYLKDN
                     PGSDLVIGEQADYIVISESEPLTRHHLDRIVKDRPLLVFSPDHHTAWANTLALEQAGI
                     LKGKALGPGNEIVMGPDGLAGGELREGEAFNPVRALLKGGDRDRLGLTTGGEPDPYPD
                     TADFESDLAVMARGLAHCAAHGLTAIHNLDGNLYTLEVLEELQRRGQLTARVRVPFHM
                     KNFMDLSMLEKASKMAARYNSDMLQSGFVKV"
     gene            complement(919..1593)
                     /locus_tag="AC16A0_09480"
     CDS             complement(919..1593)
                     /locus_tag="AC16A0_09480"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3105607.1"
                     /translation="MAATSEGEAPLYRLYWDRGSANMAPHALLRELGVPFDLVHVDVA
                     SGAHRAPGYTALNPNQRVPTLVHGNRVLYESAAILIYLCESHPESGLMPLPGDPRRHL
                     FLQWLFYFTNTLQEDLQHWWHADNYVTAEACRSDMKAVAEQRLGRFFSRIDSSLAKDG
                     PYVLGARFSACDLFFVMLCRWTRQMPVTALDYPQIKRLVDLVTQRPAWRAMMAAEGIN
                     WSGPLT"
     gene            1592..2053
                     /locus_tag="AC16A0_09485"
     CDS             1592..2053
                     /locus_tag="AC16A0_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105608.1"
                     /translation="MTRRFLLAFCLLAASALSTQAQERQWMLDASDSDAYLIFGVPDS
                     DDVGISFWCPVQQGEVNVFIPEASARLHPGADITVTLKADEESETFTGKSEVNLEAAL
                     TSVEAKVPVNAQILAAMLKADRFRVIIDGNETIFPLIDADLESLLDLCRKP"
     gene            complement(2057..2785)
                     /locus_tag="AC16A0_09490"
     CDS             complement(2057..2785)
                     /locus_tag="AC16A0_09490"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR3105609.1"
                     /translation="MIRSFLAHDFILSPNIGPRRDGRAVDMLLLHYTGMASAARAIAW
                     LCNPRAEVSCHYLVDEQGRITQMVGEEMRAWHAGQSQWRGERDTNSRSIGIEIQNTGD
                     QPYPDRQMSAVIALCRDILSRHDIPPHHVLAHSDVAPARKSDPGEMFDWERLFRNGIG
                     HWVEPEPLEQGAQLGPGDAGEAVMRLQAILAGYGYGIAVTGSYDHDTLLVVKAFQRHF
                     RPARVDGLADRSTLETLARLTAKR"
BASE COUNT          501 a          899 c          908 g          489 t
ORIGIN      
        1 agaccttcac gaagcccgac tgcagcatgt cggaattgta gcgcgccgcc atcttgctgg
       61 ccttctccag catcgacagg tccatgaagt tcttcatgtg gaacggcacg cgcacgcggg
      121 ccgtgagctg accgcggcgc tgcaactctt cgagcacttc gagcgtgtag agattgccat
      181 ccagattgtg gatggcggtc aggccatggg cggcacaatg ggcaaggccg cgcgccatga
      241 ccgcgagatc gctttcgaag tcggccgtgt cgggataggg atcgggctcg ccgccggtgg
      301 tgaggccaag ccggtcgcga tcgccgccct tcagcagcgc ccggaccgga ttgaaggctt
      361 cgccctcgcg gagctcgccc ccggccagcc cgtccggacc catgacgatc tcgttgcccg
      421 gccccagcgc cttgcccttg aggatgccgg cctgctccag cgccagcgta ttggcccagg
      481 cggtgtggtg atcgggcgag aagacgagga gcggccggtc cttcacgatg cgatccagat
      541 gatggcgggt cagcggctcg gactcggaga tgacgatgta gtccgcctgc tcgccgatca
      601 cgagatcgct tccgggattg tctttcaggt aggccagcgt gcgggatttc agctcgtcga
      661 atccctgcgt gcccgccaac tgcagatgct tgagttcggc cgcgccggca aagagatgca
      721 tgtgggcttc gacaaggccg ggcgtgacgg tggcaccccc ggcatcgatc acccgcgtcg
      781 aggccccctt ctccgctgca atctcgcgat tgctgcccac cgcggcgata cggttgccgg
      841 tgatcgccag cgcctcggcc ttgggacggg cgtcgtccat ggtgaggatg gcggcatttt
      901 cgatgacgat gtctgcactc atgtcagagg tccactccag ttgatgcctt cggccgccat
      961 catggcacgc caggcgggac gctgggtgac gagatcgacg agccgcttga tctgcgggta
     1021 atcaagtgcc gtcaccggca tctgccgcgt ccagcggcag agcatgacga agaacaggtc
     1081 gcaggccgag aaccgcgcgc caagcacata gggcccgtcc ttcgccaggc ttgaatcaat
     1141 tcgcgaaaag aatctgccaa gccgctgttc ggccacggct ttcatgtcgc tgcggcaggc
     1201 ctcggcggtg acgtagttat cggcatgcca ccagtgctgc agatcctcct gcagcgtgtt
     1261 ggtgaaatag aagagccatt gcaggaatag gtggcgccgg ggatcgccgg gcagcggcat
     1321 gagaccggat tcggggtgcg actcgcagag atagatcagg atggccgcgg attcgtagag
     1381 caccctgttg ccatgcacca atgtgggcac acgctgattg ggattaagtg ccgtgtagcc
     1441 gggcgcgcga tgggcgccac ttgccacatc gacatgaacc agatcgaagg gcacgcccaa
     1501 ctcgcgcagc agggcgtgcg gcgccatatt ggcacttccc cggtcccaat agagcctgta
     1561 gagaggcgct tcgccttcgg atgttgccgc catgacccgc cgtttcctgc tcgccttctg
     1621 ccttcttgcc gcctcggcct tgtccaccca ggcacaggag cgccagtgga tgctggatgc
     1681 aagcgactcc gatgcatacc tgatcttcgg cgtgccggac tccgacgacg tcggcatcag
     1741 cttctggtgc ccggtacaac agggcgaagt caacgtgttc attcccgaag ccagtgccag
     1801 gctgcatccg ggcgcggata tcaccgtcac cctcaaggcc gacgaagaaa gcgaaacatt
     1861 caccggcaag agcgaggtca atctcgaggc ggcgctcacc agcgtcgagg cgaaggttcc
     1921 ggtcaacgcc cagattcttg ccgccatgct gaaggccgac cgcttccgcg tgatcatcga
     1981 cggcaacgaa accatcttcc ccttgatcga tgccgacctg gaaagcctgc tggacctctg
     2041 ccgcaagccg tgagcgtcag cgcttggccg tcagccgggc cagcgtctcc agcgtcgacc
     2101 ggtcggccag gccatccact cgcgcgggcc ggaagtggcg ctggaaggcc ttcaccacca
     2161 gaagcgtgtc gtggtcatag ctgccggtaa cggcaatgcc atagccatag ccggcaagaa
     2221 tggcctgcag ccgcatgacg gcctcgcctg cgtcacccgg gccgagctgc gccccctgct
     2281 ccagcggctc aggctctacc cagtggccga tgccattgcg gaacagacgt tcccagtcga
     2341 acatttcgcc gggatcggac ttccgggccg gcgcgacatc ggaatgcgcg agaacgtggt
     2401 gagggggaat gtcgtgacgg gacaggatgt cgcggcagag cgcgatcacc gccgacatct
     2461 ggcggtccgg atagggctga tcgccggtgt tctggatctc gatgccgatg gagcgcgagt
     2521 tggtgtcgcg ctcgcccctc cactgcgact ggcctgcatg ccaggcccgc atctcctcgc
     2581 ccaccatctg ggtgatgcgc ccctgctcgt cgaccagata gtggcaggag acctcggcgc
     2641 gcggattgca cagccacgcg atcgccctgg ccgccgaagc catgcccgta tagtgtagca
     2701 gcagcatgtc caccgcacgg ccatcgcggc gcggcccgat gttgggcgag agaatgaagt
     2761 catgcgcgag gaaggagcgg atcacaggcc gcgttcc
//
LOCUS       DBNGVR010000447         2344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2914560, whole genome shotgun sequence.
ACCESSION   DBNGVR010000447 DBNGVR010000000
VERSION     DBNGVR010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2914560"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="AC16A0_09495"
     CDS             complement(<1..624)
                     /locus_tag="AC16A0_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105610.1"
                     /translation="MAAAKQSKPSLSAAELEALAADSEQGVTRRLTGWESWLASALCA
                     SLSLYALYWTQFAINTSVYRATFLGIVLAAAFVMFPIHRKLTGRHLRLLDAVLIALSV
                     ITIWYFVSHLEATKTRATAPLQIEIWLGGALMLLVLEATRRATGWALPLIAIGFLVYG
                     YFGPYMPEPFNHRGFSIARIVGQNYLTLEGLFSTPMDVAATFIIVFAL"
     gene            complement(653..1633)
                     /locus_tag="AC16A0_09500"
     CDS             complement(653..1633)
                     /locus_tag="AC16A0_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3105611.1"
                     /translation="MTHLNRRGFIAGSTFLATSLFAGVPAFAATRYITIGTGGTGGVF
                     YPYGGGLAQLLSAKLPETQVTAEVTGGSVDNVKLLSAGNAEIGFSTIDSAYDGMKGEA
                     AYKDVGPQKIATLAVLYPSFLHVVASKASGIKTIADIKGKRASVGSAGSSTESIADRM
                     LTAAGVDPMTEVTRDNLGVAESAGAIKDGKVDVFFWIGGVPTPAVKDLVSTNGDDVVF
                     VGSSDVAPKLAEAYPGVYTSGMLKKEAYANLSDDLPTLVVDNVLIVPEALDGGLVTEV
                     LTAIFDNLADVHAIHPSAKRLSLETAAAKTAVPYHPAAVEFYKSRGITAG"
     gene            complement(2086..>2344)
                     /locus_tag="AC16A0_09505"
     CDS             complement(2086..>2344)
                     /locus_tag="AC16A0_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR3105612.1"
                     /translation="LKALTFYARPDRLPLYRPNRPLDEIAHGLARACGHWGSGAAYLY
                     NTVSHLEDLGIHDPRLWELQERVAAEIERLHAGQAAGDAGR"
BASE COUNT          450 a          724 c          749 g          421 t
ORIGIN      
        1 gagcgcaaac acgatgatga aggtcgcggc cacgtccatc ggcgtcgaga acaggccctc
       61 gagcgtcagg tagttctggc cgacgatgcg cgcaatcgag aagccgcgat gattgaaggg
      121 ttcgggcatg tagggcccga aatagccata gacgaggaag ccgattgcga tcagcggcag
      181 ggcccagccg gtggctcgcc gggtggcttc cagcaccaga agcatgaggg ctccgcctag
      241 ccatatctcg atctgcaacg gcgccgtggc cctggtcttg gtcgcttcga gatgggaaac
      301 gaagtaccag atggtgatca cagacagcgc aatcagcacc gcatcgagga ggcgcaggtg
      361 gcggcccgtc agcttccggt gaatcggaaa catgacgaag gccgccgcca gcacgatgcc
      421 gaggaaggtg gcgcggtaca ccgaggtgtt gatcgcgaac tgcgtccagt agagggcgta
      481 gagcgacagc gacgcgcaaa gcgcggaggc cagccagctt tcccagccgg tgagtcggcg
      541 cgtcacgccc tgctcgctgt ctgcggccaa agcttcaagc tctgctgccg agagtgaggg
      601 tttcgactgc ttcgccgccg ccatgccgct ctcctgtcct gactggctgc gatcaacctg
      661 ccgtgatgcc gcggctcttg tagaattcca ccgccgcggg atgataggga accgccgtct
      721 tcgcggctgc cgtttcaagc gacagcctct tggccgaggg atggatggca tggacgtccg
      781 cgaggttgtc gaagatcgcc gtcaggactt cggtcaccag cccgccatcg agggcttcgg
      841 gaacgatgag cacgttgtcg acgacgagtg tcgggagatc gtcggacaga ttggcatagg
      901 cctccttctt caacattccg gacgtataga cgccgggata ggcctcggcg agtttgggag
      961 ccacgtcgct ggatccgacg aagacgacgt cgtcgccatt ggtggagacc aggtccttga
     1021 cagcaggcgt cggcaccccg ccgatccaga agaagacatc gaccttgccg tccttgatgg
     1081 cgccggcgga ctcggccacg cccagattgt cgcgggtcac ctccgtcatg ggatcgacac
     1141 cggctgccgt cagcatgcga tcggcaatgg actccgtcga agaaccggca gagcccacag
     1201 aggcccgctt gcccttgatg tcggcgatgg tcttgatgcc gctggccttg gaggccacga
     1261 catgcaggaa gctgggatag aggaccgcca gcgtggcgat cttctggggg ccgacgtcct
     1321 tgtaggctgc ctcgcccttc atgccgtcat aggccgaatc gatggtcgaa aacccgattt
     1381 cggcgttgcc ggcgctcagc agcttcacgt tgtcgaccga tccgcccgtc acctcggccg
     1441 tgacctgggt ctccggaagc ttggctgaca ggagctgcgc aagccctcca ccatagggat
     1501 agaacacgcc gcccgtacca ccggtgccga tggtgatgta gcgggtcgcg gcaaaggccg
     1561 gaacaccggc gaaaagcgaa gtcgctagga aggtactgcc ggcaatgaag ccgcgacggt
     1621 tcagatgggt catgtcgtcc tcctggttac tgttgatcga cgttctgctg tacagcccaa
     1681 atcgcaaggt aattctgctg atgggcatcg gcttgagttt gagatcgttt ccggctgggg
     1741 attcggcgcc ttgcagcggg ctcacggtca gtgtccggcc gggtcccgaa cgttgtagta
     1801 accacgcatg gcgcatcgcc cctgttcata gcagaccctc cttccgcgtc tcgcccgaga
     1861 ccgacatggc gatgaatacg gggtcgcatc tggcgaacct cctcctgcaa catctcgcac
     1921 ctcatgatcg tcccacatcc tgtctcgcaa actgcgatgc aagcccggtg ccagattccg
     1981 ctcatgcggc aggccgatct atcttactga acatgcaacg atcctgaaat cggccgagag
     2041 gcgttccggc aaacccccgc cacaaccgtc cggctgggcg gcggcctacc gcccggcatc
     2101 accggccgct tggccagcat gaagtctctc gatctcggca gcgacccttt cctgcagctc
     2161 ccacaggcgt ggatcgtgaa tgccaaggtc ttccagatga ctgacggtgt tgtagagata
     2221 ggctgcaccc gatccccaat ggccgcaggc ccgcgcgagg ccatgggcga tctcatccag
     2281 gggccggttg ggccgataga gaggcaaccg gtcgggccgt gcatagaacg taagggcctt
     2341 gagc
//
LOCUS       DBNGVR010000448         6714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4487457, whole genome shotgun sequence.
ACCESSION   DBNGVR010000448 DBNGVR010000000
VERSION     DBNGVR010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4487457"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="AC16A0_09510"
     CDS             <1..533
                     /locus_tag="AC16A0_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105613.1"
                     /translation="TTAWMILMQDGGVFAQFMNVTGITWVMNLLGVMDGPPELFKTRF
                     ATVVAMTHIQLPFTLLPIYSVMKTISPTYMRAAKSLGGTPFYSFIRVYMPQTLPGVAA
                     GCLLTFIICLGFYITPALVGGPTDQMVSGFIASAINTENNWGKASALGVILLIATLIL
                     YYVYNKLVGIDRMKLG"
     gene            539..1366
                     /locus_tag="AC16A0_09515"
     CDS             539..1366
                     /locus_tag="AC16A0_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105614.1"
                     /translation="MAVPSYFTTGEKIAHYSLRVFVMLVLLFLISPIIAVMPLSFNSE
                     SFFTYPMPGYSLKWYEDFFFNDRWTGALRMSFFIAICTTILATFLGTLAALGLARTNF
                     PAKSTIMAILISPMIVPVIISAVGMFKFYAQLDLASTLLGVILAHTALATPFVVITVT
                     ATLSAFDKTLMRAGASCGAPPHTVFFKVVMPLILPGMISGALFAFVTSFDEVVVVLFL
                     ASPEQRTLPLQMFSGIREMISPTITAAATVLVLFSTALLVTVELLRRRSEKLRGVQA"
     gene            complement(1363..1689)
                     /locus_tag="AC16A0_09520"
     CDS             complement(1363..1689)
                     /locus_tag="AC16A0_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105615.1"
                     /translation="MTARRGSVDGICFAAEGMAGTAAATSLRRFQSLADAAPSGRLFA
                     AITTLTELSTQSSRALEDGSKALADGDEHTATVKLARALTHMDDLATASTVQSEQKPG
                     FLRRAR"
     gene            1820..2569
                     /locus_tag="AC16A0_09525"
     CDS             1820..2569
                     /locus_tag="AC16A0_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3105616.1"
                     /translation="MAGHSQFKNIMHRKGKQDKMRSKIFSKLSREITVAAKAGLPDPT
                     ANARLRLAIQNARAENLPKDNIERAIKKAQGGDAESYDSVRYEGYGPGGVAVIVEALT
                     DNRNRTASNVRALFTKYGGNLGESGSVAFMFSRVGEILYPVAKASADAMLEAAIDAGA
                     DDAASDESGHTITCAFESIGEVSSALAAKFGDAESVKVVWKPQTLTPVDQEKAESLMK
                     LVDGLDEDDDVQVVFSNADISDEIMAKLGGE"
     gene            2587..3090
                     /gene="ruvC"
                     /locus_tag="AC16A0_09530"
     CDS             2587..3090
                     /gene="ruvC"
                     /locus_tag="AC16A0_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3105617.1"
                     /translation="MVRIIGIDPGLRNTGWGVIASDGQRLSYVADGSVHSVADAPLAV
                     RLLQVHTQLMAVIRAHEPDEAAVEETFVNTDARATLKLGQARGVVMLAPAMLSIPVSE
                     YAPNTIKKSVVGAGHADKDQVKHMVKLLLPRAQLNSADSVDALAIAICHAHHRTGQTL
                     ARLLARS"
     gene            3138..3755
                     /gene="ruvA"
                     /locus_tag="AC16A0_09535"
     CDS             3138..3755
                     /gene="ruvA"
                     /locus_tag="AC16A0_09535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3105618.1"
                     /translation="MIAKLKGRIDACGADWVVIDVNGVGYHVACSSRTLQALPGQGEF
                     AEIHTEMLVSQDMIRLVGFASTLERDWFRLLQSVQGVGTKVALAILSTLSVHEVGNAI
                     AMQDKAMIGRANGVGKKLAERIVLELKDKTPSFAAADAALAKLQADLDAPQPTAAADA
                     VSALVNLGYGQTQAGAAVAAALRKAGDDQPTEKLIRLALKELALA"
     gene            3752..4795
                     /gene="ruvB"
                     /locus_tag="AC16A0_09540"
     CDS             3752..4795
                     /gene="ruvB"
                     /locus_tag="AC16A0_09540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR3105619.1"
                     /translation="MNTRLTGREKIPEDAIDTHLRPQRLEEFVGQSKAKQNLRVFIAA
                     AKARGEALDHVLFAGPPGLGKTTLAQIMSRELGVNFKATSGPVIAKAGDLAALLTNLE
                     ERDVLFIDEIHRLNPAVEEVLYPAMEDFQLDLIIGEGPAARSVRIDLAKFTLVGATTR
                     TGLLTTPLRDRFGIPVRLDFYAEDELLEIVKRGARVMQVSVSEDGAREIAKRSRGTPR
                     IAGRLLRRVRDFAAVAGTATIDRAAADRALRSLDVDDRGLDALDHRYLACIAGNFGGG
                     PVGIDTIAASLSEARDAIEDIVEPYLLQLGFLNRTPRGRLLTAHAFRHLGLAVPQRPE
                     IAQGLLPLDGGDD"
     gene            4788..5231
                     /gene="ybgC"
                     /locus_tag="AC16A0_09545"
     CDS             4788..5231
                     /gene="ybgC"
                     /locus_tag="AC16A0_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIR3105620.1"
                     /translation="MTERWPHIAGYSRGGLHVLPVRVYYEDTDAGGVVYHANYLKYCE
                     RGRTDFLRLRGVHQSAMKDAFFVVRRMSCDFLRPARLDDLLEVETRLAETGGARLVLD
                     QRVNRDGTPIFQAQVTVALIDARGRPRRFAPDLLLALNSRGESGS"
     gene            5431..6138
                     /gene="tolQ"
                     /locus_tag="AC16A0_09550"
     CDS             5431..6138
                     /gene="tolQ"
                     /locus_tag="AC16A0_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR3105621.1"
                     /translation="MDPVAVETVAQTGHISLWHLVMQASWVVKLVMLGLALSSVWSWA
                     IIIEKFFAMRKMNAANDRFEQNFWSGQSLEDLYISLGNRPTVGLAAIFMAAMREWKRS
                     QEAAMRAGFHGVQKRIEKVMDVQIQKEMHDVEARLLFLATTGSAAPFIGLFGTVWGIM
                     VSFQAIANSQNTNLAVVAPGIAEALFATAIGLVAAIPATIFYNMFTADTGRIGSRLEN
                     FADEFSAIISRQLDERA"
     gene            6141..6608
                     /gene="tolR"
                     /locus_tag="AC16A0_09555"
     CDS             6141..6608
                     /gene="tolR"
                     /locus_tag="AC16A0_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR3105622.1"
                     /translation="MGASTGGSAGGYQRRNSRRRGAGRGVISEINVTPLVDVMLVLLI
                     VFMVAAPLMTVGVPIELPKTEAKQMDSSTEPITITVQQDGALFLQETAIPLAELASKL
                     TAIAKAGYEEQLFVRADTSVPYGRVMEVMGLLNSAGYRKIGLITATKDEGPAN"
BASE COUNT         1133 a         2251 c         2142 g         1188 t
ORIGIN      
        1 gcaccaccgc ctggatgatc ctcatgcagg acggcggcgt cttcgcgcag ttcatgaacg
       61 tcacgggcat cacctgggtc atgaacctgc tgggtgtcat ggacggcccg ccggagctgt
      121 tcaagacgcg cttcgccacc gtggtggcca tgacccacat ccagctgccc ttcacgctgc
      181 tgcccatcta ctcggtgatg aagaccatct cgcccaccta catgcgggcg gcgaagtcgc
      241 tggggggcac gcccttctac tccttcatcc gggtctacat gccgcagact cttcccggcg
      301 tggcggcggg ctgcctgctc accttcatca tctgcctcgg cttctacatc acgcccgcgc
      361 tggtcggcgg ccccaccgac cagatggtgt cgggcttcat cgcctcggcg atcaacaccg
      421 agaacaactg gggcaaggct tcggcgctgg gcgtcatcct gctcatcgcc acgctgatcc
      481 tgtattacgt ctacaacaag ctggtcggca tcgaccgcat gaagctgggc tgagcgcgat
      541 ggccgtaccg tcctatttca ccaccggcga gaagatcgcg cattacagcc ttcgcgtctt
      601 cgtgatgctg gtgctgctgt tcctgatctc gccgatcatt gcggtgatgc cgctctcctt
      661 caactccgag agcttcttca cctatcccat gcccggctat tcgctgaagt ggtacgagga
      721 cttcttcttc aacgatcgct ggaccggcgc gttgcgcatg agtttcttca tcgccatttg
      781 caccaccatc ctcgccacct tcctcggcac gctggcggcc ctcggcctcg cacgcaccaa
      841 ctttcccgcg aagtcgacga tcatggccat cctgatctcg ccgatgatcg ttccggtgat
      901 catctcggcg gtcggcatgt tcaagttcta tgcgcagctc gacctggcca gcacgcttct
      961 cggcgtgatc ctcgcgcaca cggcgctggc aacgcccttc gtggtgatca cggtcaccgc
     1021 cacgctctcg gccttcgaca agacgctgat gcgcgccggc gcgtcctgcg gggcgccgcc
     1081 acacacggtc ttcttcaagg tggtgatgcc gctgatcctg cccggcatga tctcgggtgc
     1141 gctcttcgcc ttcgtcacat ccttcgacga agtcgtggtg gtgctgttcc tcgccagccc
     1201 cgagcaacgc accctgccgc tgcagatgtt ctctggaatc cgagaaatga tctcgcccac
     1261 catcacggca gcggctaccg tgctggtgct gttctcgacg gccttgctgg tgacggtcga
     1321 actgttgcgc cgccgctcgg agaaactgcg cggcgtgcag gcctagcgcg cgcggcgcag
     1381 gaagcccggc ttctgctcgg attgaaccgt ggacgccgtt gccagatcgt ccatgtgggt
     1441 gagggcgcgg gccagcttga ccgtggccgt gtgctcatcg ccatcggcca gtgccttgct
     1501 gccgtcctcc agcgcgcggc tggactgcgt cgagagttcg gtcagcgtcg tgatcgcggc
     1561 aaagagccgg cccgaaggtg cggcatcggc aagcgactgg aagcggcgca gcgatgtcgc
     1621 agcggccgtg cccgccatgc cttccgctgc aaaacagata ccgtccaccg aaccccgccg
     1681 tgccgtcatt gcaaactcct taacgcgact gacggccgga gcttggctcg cccaggctta
     1741 attccgggtt aagcggcctt gtcttgcccg agccccactg gttataaggc agcccgcaat
     1801 ttccccatcc gaggacgtca tggccggcca ttcgcaattc aagaacatca tgcaccgcaa
     1861 ggggaagcag gacaaaatgc ggtccaagat cttctccaag ctgtcgcgcg agatcacggt
     1921 ggcggccaag gcgggcctgc ccgatcccac cgccaatgcc cggctgcgcc ttgccatcca
     1981 gaacgcccgc gccgagaacc tgcccaagga caacatcgag cgggccatca agaaggcgca
     2041 gggcggcgat gccgagagct acgattccgt gcgctacgag ggttacggcc ccggcggcgt
     2101 cgccgtcatc gtcgaggcgc tgaccgacaa ccgcaaccgc accgcgtcga atgtccgcgc
     2161 gctcttcacc aagtacggcg gcaatctcgg cgaatccggt tcggtcgcct tcatgttcag
     2221 ccgggtgggc gagatcctct atcccgttgc caaggcctcg gccgatgcca tgctggaggc
     2281 ggcgatcgat gcgggggccg acgatgccgc ctccgacgag tcggggcaca ccatcacctg
     2341 cgctttcgag tcgatcggcg aggtgtcctc cgccctggcc gcgaagttcg gcgacgctga
     2401 aagcgtgaag gtcgtgtgga agccgcagac cctgacgccg gtcgatcagg agaaggccga
     2461 aagcctgatg aagctggtcg acggcctcga cgaggacgac gacgtgcagg tggtcttctc
     2521 caacgccgac atctcggacg agatcatggc caagctcggc ggcgagtgat ctgctaggct
     2581 tggccgatgg tgcgaatcat cggcatcgat ccaggcctgc gcaacaccgg ctggggcgtg
     2641 attgcctcgg acggccagcg gctctcctat gtggccgacg gttcggttca ctccgttgcc
     2701 gatgcgccct tggccgtacg cctgctgcag gtccacacgc agctcatggc cgtcatccgc
     2761 gcccatgaac cagatgaagc ggcggtggag gaaaccttcg tgaacaccga tgcccgcgcc
     2821 acgctgaagc tgggccaggc ccgcggggtg gtgatgctgg cgcccgccat gctgtccatt
     2881 cctgtctccg aatatgcgcc gaacaccatc aagaagtcgg tggtgggggc agggcacgcg
     2941 gacaaggacc aggtcaagca catggtgaaa ctgctgctgc cgcgggcgca gctcaacagc
     3001 gcggactcgg tcgacgcctt ggccatcgcc atctgccatg cccaccatcg gacaggccag
     3061 acactggccc ggctgctggc gcgttcttga cttgttccgg cttgggcagt agcagggcat
     3121 agcaacggga cttgccgatg atcgcaaaac tcaagggccg gatcgacgcc tgcggagccg
     3181 actgggtggt gatcgacgtg aacggcgtgg gctatcacgt cgcctgctcg tcgcgcacgc
     3241 tgcaggccct gccgggccag ggcgagttcg cggaaatcca caccgagatg ctggtcagcc
     3301 aggacatgat ccgcctcgtc ggctttgcct ccacgctcga gcgcgactgg ttccgcctgc
     3361 tgcagtcggt gcagggggtc ggcaccaagg tggcgctggc cattctctcc acactctcgg
     3421 tgcatgaggt gggcaatgcc atcgccatgc aggacaaggc catgatcggc cgcgccaatg
     3481 gcgtgggcaa gaagctggcc gagcgcatcg tgctcgaact caaggacaag acgcccagct
     3541 tcgccgccgc cgatgccgcc ctggcgaagc tgcaggccga cctcgacgca cctcagccca
     3601 ccgccgcggc ggacgcggtt tcggcgctgg tcaaccttgg ctatggccag acccaggccg
     3661 gagcggccgt ggccgcggcc ctgcgcaagg cgggcgatga tcagcccacg gagaaactta
     3721 tccggctggc gctcaaggaa ctggcgctgg catgaacacc cgtctcaccg gccgcgagaa
     3781 gatcccggaa gatgccatcg acacgcattt gaggccgcag cggctggagg aattcgtcgg
     3841 ccagtccaag gccaagcaga acctgcgcgt cttcatcgcg gcggccaagg cgcgcggcga
     3901 ggcgctggat cacgtgctct tcgccggccc gcccggcctc ggaaagacga cgctggcgca
     3961 gatcatgtcg cgcgagctgg gcgtcaactt caaggccacc tcgggccccg tcatcgccaa
     4021 ggcgggcgat ctcgcggcgc tgctcaccaa tctcgaagag cgcgacgtgc tcttcatcga
     4081 cgagatccat cgcctgaacc cggcggtgga ggaggtgctc tatccggcga tggaggattt
     4141 ccagctcgac ctcatcatcg gcgaggggcc tgcggcccgc tcggtccgca tcgaccttgc
     4201 gaagttcacc ctggtcggtg ccacgacgcg taccggcctt ctcaccacgc ccctgcgcga
     4261 ccgcttcggc attcccgtcc ggctcgactt ctacgccgag gacgagctgc tcgagatcgt
     4321 gaagcgcggc gcgcgggtca tgcaggtgag cgtctcggaa gatggcgcgc gcgagatcgc
     4381 caagcgttcg cgcggcacgc cccgcatcgc cggacggctg ttgcgccggg tgcgcgattt
     4441 tgccgccgtg gccggcaccg cgaccatcga ccgcgccgcg gccgacaggg cgctgcgcag
     4501 cctcgatgtt gacgaccgcg ggctggatgc cctcgaccac cgctatcttg cctgcattgc
     4561 cggcaacttc ggcggcggcc cggtgggcat cgacaccatc gccgcctcgc tgtccgaagc
     4621 ccgcgacgcc atcgaggaca tcgtcgagcc ctatctgctg cagctgggct tcctcaaccg
     4681 cacgccgcgc gggcggctgc tgaccgccca tgccttccgc catctgggcc tcgcggtgcc
     4741 gcagcgtccg gagattgccc agggcctgct gcccctggac ggcggcgatg actgagcgct
     4801 ggccccatat cgccgggtac agccggggcg ggctgcatgt gcttccggtg cgggtctatt
     4861 acgaggatac cgacgccggc ggcgtggtct atcacgccaa ctatctgaaa tactgcgagc
     4921 gcggccggac cgatttcctc cgcctgcgcg gcgttcatca gagcgcgatg aaggatgcct
     4981 ttttcgtggt ccgccgcatg agctgcgatt tcctgcgccc ggcgcggctc gacgacctgc
     5041 tcgaggtcga gacgcggctg gccgaaacag gcggggcccg cctcgtgctc gaccagcggg
     5101 tgaaccgcga cggcaccccg atctttcagg cccaggtgac ggtcgcgctg atcgatgcgc
     5161 gggggcggcc gcgccggttt gcgccggact tgctgctcgc gctgaattcc cgtggggaat
     5221 caggatcgta acacgacctt aaccatgttt ccgtcaagtg gaggcagagc gggctgggaa
     5281 cggccggctc cactgcggtt ttgggcataa gtgcccgaat cgccgcagtt tcgacagatt
     5341 tggcatgttt cagggcacat ctcccaaagg gtgtggtgcg caggggcggc ggaagctgaa
     5401 ttggcttccg ggcgcggatg gaaggacagg atggatccgg tagcggtcga aacagttgcg
     5461 cagacagggc acatttcgct ctggcacctc gtcatgcagg cgagctgggt cgtcaagctg
     5521 gtcatgctgg ggcttgccct gtcgtcggtg tggagctggg ccatcatcat cgagaagttc
     5581 tttgccatgc ggaagatgaa cgccgccaat gaccgtttcg agcagaattt ctggtcgggc
     5641 cagtcgctcg aggatctcta catctcgctg ggcaaccggc cgaccgtcgg gctggcggcc
     5701 atcttcatgg cggccatgcg cgagtggaag cgcagccagg aagcggccat gcgggccggc
     5761 ttccacggcg tccagaaacg catcgagaag gtgatggacg tgcagatcca gaaggagatg
     5821 cacgacgtcg aggcgcgcct gctgttcctg gcgaccaccg gctcggccgc gcccttcatc
     5881 ggccttttcg gcacggtctg gggcatcatg gtctccttcc aggccatcgc caactcgcag
     5941 aataccaatc tcgcggtggt ggcgcccggc atcgccgagg cgctctttgc cacggccatc
     6001 ggcctcgttg ctgccatacc ggcgacaatc ttctacaaca tgttcaccgc cgacacgggc
     6061 cgcatcggct cgcggctgga gaatttcgcc gatgaattct cggcaatcat ctcgcggcaa
     6121 ctggacgagc gggcatagcg atgggtgctt cgacaggcgg atcggcgggc ggttaccagc
     6181 ggcgcaactc caggcggcgg ggtgccggcc gcggcgtcat cagcgagatc aacgtgacgc
     6241 cgctggtcga cgtcatgctg gtgctgctca tcgtcttcat ggtggccgcg ccgctgatga
     6301 cggtgggcgt gccgatcgaa ctccccaaga ccgaagccaa gcaaatggac tcctccaccg
     6361 aacccatcac catcaccgtt cagcaggatg gcgcgctgtt cctgcaggag accgcgatcc
     6421 cgcttgccga actcgcctcc aagctgacgg ccatcgccaa ggccggctac gaggagcagc
     6481 tcttcgtgcg tgccgacacc agcgtgccct atggccgggt catggaagtg atgggcctgc
     6541 tcaacagcgc cggctatcgc aagatcgggc tcatcaccgc caccaaggac gaggggccgg
     6601 caaactgaga tgaagtacag cctcaccacg tcgctcctgc tgcacgcgtc gatcctgatc
     6661 gccgcgctgg tgacgttgcc ggaggccaac ccctacaaga tcaagccgca ggaa
//
LOCUS       DBNGVR010000449         3070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5079331, whole genome shotgun sequence.
ACCESSION   DBNGVR010000449 DBNGVR010000000
VERSION     DBNGVR010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5079331"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="AC16A0_09560"
     CDS             <1..795
                     /locus_tag="AC16A0_09560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3105623.1"
                     /translation="AMMGLKTLAVAGVGDDLMGDWVLQRLQHFGVDTWGVQRKPGWKT
                     SSSIVTTRADGSRPALHMKGATANFFVDDSMIPRVVDTKVFHAGGVGLMDAMDKGQSA
                     IVMKAAKAGGATTTVDVFAGSPKDLPAIAQVLPYTDYFMPSIEEAQALCGLTDHGDTA
                     RFFLDMGVKACVLTLGGDGAYYHDRDGLKFHVPAFDIAVKCTCGCGDAFDAGFAVALN
                     HDFDAETSVRFAQATSALNATGLGSQAGVKDFAHTLAFMKTTKTKR"
     gene            complement(799..1578)
                     /locus_tag="AC16A0_09565"
     CDS             complement(799..1578)
                     /locus_tag="AC16A0_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105624.1"
                     /translation="MTSMSRTADSLAAHAVCAAAGMRDGLRAAQFYVRDLSNADRFAK
                     GEPGRAPFLSRAVAQGARLVDGFMSWSANLAIRLFLPSWRKLPSPFTPGIMHEVARGI
                     RENSLMQNPLFNAYFFRAAIHIAGRYSQPPYLILEHRIDAARRQLAAQIDDGASENEV
                     LARILIALVEQGPVARIGAIEGHNRFFGLVDPNIAVNAIACVALMFAEEGKPAQAIDE
                     DSFFSVTGALVGPRLNTIATLVARRDTPGLAREFADIKTLY"
     gene            complement(1589..2416)
                     /locus_tag="AC16A0_09570"
     CDS             complement(1589..2416)
                     /locus_tag="AC16A0_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3105625.1"
                     /translation="MSEKSASSLFRTVILLAWGLFCLMPIIFFATIALRPRNEIISPQ
                     PIYFPTISIETWAKIWAEWPIMTYFMNSVIAVSGSVVICLVLGIPAAYSLARYRYRGR
                     EDIGFYILSTKMMAPAAVAIPIFFLFRSMGLLDTVWALMLVYAAINLSIVVWIIRSYM
                     IDIPHEIEDAARTDGASDLRILWSIIIPMCLPGIITAAVIAAIFAMNEYLFTLIIASK
                     QAYPLSVALANFSGGSDGIIYNAIALVAFLAFFPALLLVILIQNHLSRGLTMGAVKG"
     gene            complement(2416..>3070)
                     /locus_tag="AC16A0_09575"
     CDS             complement(2416..>3070)
                     /locus_tag="AC16A0_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3105626.1"
                     /translation="QGDRGGRAAGRRALALAALVYRDRWVQGWRILFLLPMLFMPSAV
                     AFLWKLLFNDGRIISDLLMRIGLADGNIDFMGSTWLARMVLIVTDVWQWVPFLFIIFV
                     AALQGQDKEIEEAARLDGARWRQIFFAISLPMMRPVIAVALVLRGIDILTMFTSVHVI
                     TQGTPAGNTETISYFIYRTAFKSFEFGYASAASVVVLVITVILAQSFVRRFFHSGRD"
BASE COUNT          611 a          941 c         1041 g          477 t
ORIGIN      
        1 gccatgatgg ggctgaagac gctggcggtc gcaggcgtgg gcgacgacct gatgggcgac
       61 tgggtgctgc agcgattgca gcacttcggc gtcgacacct ggggcgtgca gcgcaagccc
      121 ggctggaaga cctcctcctc catcgtcacc acgcgtgccg acggttcgcg gcccgccctg
      181 cacatgaagg gcgctaccgc caatttcttc gtcgacgaca gcatgatccc gcgtgttgtc
      241 gataccaagg tgttccatgc cggcggcgta ggcctgatgg acgccatgga caaaggccag
      301 agcgcgattg tcatgaaggc agccaaggcc ggcggcgcca caaccaccgt ggacgtcttc
      361 gccgggtcgc cgaaggacct gccggccatc gcgcaggttc ttccctatac ggattacttc
      421 atgccctcga tcgaggaagc gcaggcgctc tgcggcctga cggaccacgg cgatacagcg
      481 cgcttcttcc tcgacatggg ggtcaaggcc tgcgtgctca cgctgggcgg cgacggcgcc
      541 tactaccatg acagggacgg gctcaagttc catgtcccgg ccttcgacat cgccgtcaaa
      601 tgcacctgcg gctgcggcga tgccttcgat gccggtttcg ccgtggcgct caaccacgac
      661 ttcgatgcgg agacatccgt gcgctttgcg caggcgacct cggcgctcaa tgccaccggt
      721 ctcggctcgc aggcgggggt caaggacttt gcacacacgc tcgccttcat gaagaccacg
      781 aagacgaagc ggtaggcctc agtacagcgt cttgatgtcg gcgaactcgc gcgccagacc
      841 gggcgtgtcg cgccgcgcca ccagggtcgc aatggtgttc aggcgcggtc ccacgagtgc
      901 gccggtcacc gagaagaacg agtcctcgtc gatggcctgc gcgggtttcc cctcttccgc
      961 gaacatcagg gcgacgcagg caatggcatt gaccgcgatg ttcgggtcga caaggccgaa
     1021 gaagcggttg tggccctcaa tggccccgat ccgtgccacc ggcccctgct ccaccagcgc
     1081 gatcaggatt cgcgccagga cctcgttctc cgatgcccca tcgtcgatct gcgccgccaa
     1141 ctggcgccgg gccgcgtcga tgcgatgctc gagaatgagg tagggcggct gcgaataacg
     1201 gccggcgatg tgaatcgccg ccctgaagaa ataggcgttg aacagcggat tctgcatcag
     1261 gctgttctcg cggatgccgc gggccacctc gtgcatgatg ccgggtgtga agggcgaggg
     1321 cagcttccgc cagctgggaa ggaagaggcg gatggcgagg ttggcactcc agctcatgaa
     1381 gccgtcgacc agccgggccc cttgggccac ggcgcgcgac aggaagggcg ccctgcccgg
     1441 ctcgcccttg gcgaagcggt cggcattgga caggtcgcgg acatagaact gggccgcgcg
     1501 cagcccgtcg cgcatgccgg cggcggcgca gacggcgtgg gcggcaagcg aatcggccgt
     1561 acgggacatg ctggtcatga cgccaatatc agccctttac ggcacccatc gtcaagccgc
     1621 gcgacaaatg gttctgaatc aggatgacca ggagcagcgc ggggaagaag gccaggaagg
     1681 ccaccagggc gatggcattg tagatgatgc cgtcggagcc gcccgagaag ttggccaggg
     1741 ccaccgagag cggataggcc tgcttggagg cgatgatcag ggtaaacaga tactcgttca
     1801 tggcgaagat ggccgcgatg accgccgccg tgatgatgcc gggcaggcac atgggaatga
     1861 tgatcgacca gaggatgcgc agatccgaag ccccgtcggt gcgcgcggca tcctcgatct
     1921 cgtgcggaat gtcgatcatg taggagcgga tgatccagac gacgatggac aggttgatgg
     1981 cggcatagac cagcatgagg gcccagacgg tgtcgagcag gcccatgctg cggaacagga
     2041 agaagatcgg aatggcgacc gccgccggcg ccatcatctt ggtcgacagg atgtagaagc
     2101 cgatgtcctc gcgcccgcga tacctgtagc gggccagcga ataggccgcc ggaatgccca
     2161 gcacgaggca gatgaccacg ctgccgctga ccgcgatcac cgagttcatg aaataggtca
     2221 tgatcggcca ttcggcccag atcttggccc aggtctcgat cgagatggtc gggaagtaga
     2281 tcggttgcgg cgaaatgatc tcgttgcgcg gacgcagcgc gatggtggcg aagaagatga
     2341 tgggcatcag gcagaagagg ccccaggcca gaaggatgac cgtccggaac agcgaggatg
     2401 cggacttctc ggacatcagt ccctccccga atggaagaag cggcgcacga agctctgggc
     2461 caggatcacc gtgatcacca gcacgaccac cgaggcggcc gaggcatagc cgaattcgaa
     2521 gctcttaaag gccgtgcggt agatgaaata gctgatcgtc tcggtgttgc cggcaggggt
     2581 gccctgcgtg atgacgtgca cggaggtgaa catcgtcagg atgtcgatgc cgcgcagcac
     2641 cagggcgacc gcgatcaccg gccgcatcat ggggagcgag atggcaaaga agatctggcg
     2701 ccagcgcgcg ccgtccagcc gcgcggcctc ctcgatctcc ttgtcctggc cctgcagcgc
     2761 ggccacgaag atgatgaaga ggaagggcac ccattgccac acgtcggtga cgatcagcac
     2821 catgcgggca agccaggtcg agcccatgaa gtcgatattg ccgtcggcca gcccgatgcg
     2881 catcagcagg tcactgatga tgcggccatc gttgaagagc agcttccaga ggaaggccac
     2941 ggcggacggc atgaagagca tgggcaagag aaacaggatg cgccagccct gcacccagcg
     3001 gtcacggtag accagcgcgg cgagcgccag cgcgcgccgt ccagccgcgc ggcctcctcg
     3061 atctccttgt
//
LOCUS       DBNGVR010000450         3506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2327697, whole genome shotgun sequence.
ACCESSION   DBNGVR010000450 DBNGVR010000000
VERSION     DBNGVR010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2327697"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="AC16A0_09580"
     CDS             complement(<1..2157)
                     /locus_tag="AC16A0_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3105627.1"
                     /translation="MAERSFAREVEDLRLGAGEEFRGEGILAITKALLQSGVAYVGGY
                     QGAPVSHLMDVLADASGYMAELGVHFESSASEAAAAAMLSASVMYPIRGAVTWKSTAG
                     TNVASDALSNLSSGGVTGGAVIVIGEDYGEGSSIMQERSHAFAMKSQIWLADPRPDLP
                     TMVRIVEKSFELSEASNTPVMVEVRIRACHVHGRFVAKDNLRPRYTLREALENPQRDV
                     NRIVLPPASFSHERLKIEKRWPAAVAFIRSEKLNERFGPADGDVGIILQGGMYNGVIR
                     ALQQLGLSDAYGATRMPLHVLNITYPLVPEEVIDFCRDKRAVLIVEEGQPEFIEQAIS
                     TLLRRAELETRLHGKDMLPMAGEYTATVLRTGIARFLEEAAPALLGNRPPFSDTSKLL
                     QSAEIRKLAEEVPPRPPGFCTGCPERPIFAAMKLVEKELGAHHVSADIGCHLFSILPP
                     FNIGTTTMGYGLGPAGASAFNVPAGKRAISVMGDGGFWHNGLATSVGNAVYNKHDGVM
                     LIVDNYYSAATGGQDIPSSRAISPKRQTNNAIADAVRGIGATWVRQIDRTYDVARMRD
                     TLREALTTDAPGPKIIVAASECMLNKQRRERPAREREERDGRPVLKQRFGVDEDICTG
                     DHACIRLSGCPSLSVRVTDDPLRDDPVATIDNSCVACGNCGEVADAAVLCPSFYRADV
                     LRNAPRWSRLWDRLRRQIITALQSRREAGRALFDRTT"
     gene            complement(2297..2372)
                     /locus_tag="AC16A0_09585"
     tRNA            complement(2297..2372)
                     /locus_tag="AC16A0_09585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2337..2339),aa:Val,seq:cac)
     gene            complement(2414..3091)
                     /locus_tag="AC16A0_09590"
     CDS             complement(2414..3091)
                     /locus_tag="AC16A0_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3105628.1"
                     /translation="MPDRAVIFDLDGTLVDTAPDLMRATNHVLEAIGRRPITMDEVRV
                     FVGHGARALLTRGLAATGGLPENYDVEPDYRRFVDFYAANIAGGSLPFPGTVALLDRL
                     KAENIAMGVCTNKLEGLSLRLLEELDLLKYFGSVVGPDTIGIAKPDPRPFHESVARLG
                     RKNPRAIMIGDSETDILTARNAGVPVIGVPFGYTPQHVSAFGPDRVIGHYDEAWDALQ
                     ELFAQEA"
     gene            3173..>3506
                     /locus_tag="AC16A0_09595"
     CDS             3173..>3506
                     /locus_tag="AC16A0_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3105629.1"
                     /translation="MDIRKAQALRDILKDAALLKEKCYIDGKWVGGKTTIDVTNPVDE
                     SVIATVPKLGAAETRAAIEAAQRAQKDWAKKTAKERAAILRKWFNLMMENQEDLAQIM
                     TAEQGKPLA"
BASE COUNT          676 a         1056 c         1155 g          619 t
ORIGIN      
        1 cgtcgtccgg tcgaagaggg cgcggccggc ctcgcgccgg gattgcaggg cggtgatgat
       61 ctgcctgcgg agccgatccc ataaacgcga ccagcgggga gcattgcgca ggacgtcggc
      121 ccgatagaag gaggggcaaa gaaccgcagc gtcggcaact tcgccgcaat tgccgcaggc
      181 cacgcagctg ttgtcgatgg tggccaccgg gtcatcgcgc aaagggtcgt cggtgaccct
      241 caccgaaagc gaggggcatc cggaaagccg gatacatgcg tgatctcccg tgcagatatc
      301 ctcgtcgaca ccgaaacgct gcttcaggac cggccgcccg tcgcgttctt cccgttcgcg
      361 cgcggggcgt tcccggcgtt gtttgttcag catgcattcg gacgcggcga cgatgatctt
      421 ggggccaggc gcatccgtgg tcagcgcctc gcgcagggtg tcacgcatgc gcgccacgtc
      481 ataggtgcga tcaatctggc ggacccatgt cgcgccgatt cccctcacgg cgtccgcgat
      541 cgcattgttg gtctggcgct tcggcgaaat tgccctcgac gaggggatgt cctgaccgcc
      601 ggtcgcagca gagtagtagt tgtcgacgat gagcatcacc ccgtcatgct tattgtacac
      661 cgcattgccc accgaggttg cgagaccatt atgccaaaag ccgccatctc ccatgaccga
      721 gatggcgcgc tttcccgccg gaacgttgaa ggcagacgcg cctgcgggac cgagcccata
      781 gcccatggtg gtggtgccga tgttgaaggg cggcaggatc gagaagagat ggcagccgat
      841 gtcggccgag acatgatggg ccccaagttc cttctcgacc agcttcatag ccgcgaagat
      901 cggccgctcg gggcagccgg tacagaagcc gggagggcgc ggcggcacct cttcagcaag
      961 cttccgaatc tcggctgact gaagaagctt cgatgtgtcc gaaaatggtg gacgattgcc
     1021 gaggagggca ggggcagctt cttccaggaa gcgggcgatg ccggtccgga ggaccgtggc
     1081 ggtgtattca cccgccatcg gcagcatgtc cttgccatgc agccgcgttt caagttctgc
     1141 gcggcgcaac aaggtgctga tggcttgctc gatgaattcg ggctggccct cctcgacaat
     1201 cagaacggcg cgcttatccc ggcagaagtc gatcacctcc tcgggaacca gcggataggt
     1261 gatgttgaga acatggagcg gcatgcgcgt cgcgccataa gcatccgaca ggccaagctg
     1321 ttgcagggcg cggatgacac cattatacat gccgccctgc aggatgatgc cgacgtcgcc
     1381 gtccgccggt ccgaagcgct cgttgagttt ctccgagcgg atgaaagcca ccgccgcggg
     1441 ccagcgtttc tcgatcttga gcctttcgtg gctgaaggac gcgggcggca gaacgatccg
     1501 gttcacgtca cgctgtgggt tctccagcgc ctcgcgcagg gtgtatcgcg gcctcagatt
     1561 gtccttcgcg acgaacctgc catggacatg gcaggcgcgg atgcggactt cgaccatgac
     1621 cggcgtattg gaggcttccg acagttcgaa ggacttctcg acgatccgta ccatggtcgg
     1681 gaggtccgga cgtggatcgg ccagccagat ctgggacttc atcgcgaagg catggctgcg
     1741 ctcctgcatg atggatgagc cttcgccata gtcttctccg atgacgatca cggcaccgcc
     1801 ggtgacgccg ccggatgaca ggttggacag ggcatcgctg gcaacattcg ttcctgcggt
     1861 cgatttccag gtgaccgcgc cgcggatcgg atacatgacc gaggccgaga gcatagcggc
     1921 ggccgcggcc tcggaggccg aactttcaaa gtgaacgccg agttccgcca tgtaaccgga
     1981 tgcgtcggcg agcacatcca tcaggtggga aacgggagcg ccctggtatc cgcccacgta
     2041 ggcgacgccg gactgcagca acgccttggt aatggcgagt atgccctcgc cgcggaactc
     2101 ctcgcccgcg cccagccgca ggtcttccac ttctcgtgca aaggagcgct cggccatggg
     2161 ctaagtctcg gggtccatat atttgcagat tcatataact gtgcaagccg tgcgtcaact
     2221 ggccttgcaa aacaagcaac cacatgcatt cagatgcaag tgttttgcct ggcggaactt
     2281 gtcgggagat taccggtggt gggcgatact ggactcgaac cagtgacccc tgcgatgtga
     2341 acacagtgct ctaccaactg agctaatcgc ccgttcaggg tggaggccgt ataggcggcg
     2401 gggcttggcc aagtcaagct tcttgcgcaa acagctcctg cagcgcgtcc caggcctcgt
     2461 cgtagtggcc gatcacccgg tccggcccga aggccgagac atgctggggc gtatatccga
     2521 agggaacgcc gatgaccggc acaccggcat tgcgggcggt aaggatgtcg gtttcgctgt
     2581 cgccgatcat gatggcgcgc gggttcttgc ggccaaggcg cgcaaccgat tcatggaagg
     2641 gacgggggtc gggcttggcg atgccgatcg tgtcggggcc gacgacggag ccgaagtatt
     2701 tcagcagatc gagttcttcg agaaggcgca aggatagccc ctcgagcttg ttggtgcata
     2761 cccccatggc gatgttctcc gccttcaacc ggtcgagcag ggcgacggtt ccggggaagg
     2821 gcaggctgcc gcccgcgatg ttggccgcat agaagtcgac gaagcgccgg taatcgggct
     2881 cgacgtcata gttctctggc aagccgcccg tggcggcgag gccgcgggtg agcagcgcac
     2941 gggccccgtg gcccacgaag acccggacct cgtccatcgt gatgggccgc cggccgatcg
     3001 cttcaagcac atgattggtg gcgcgcatga ggtcgggcgc ggtgtccaca agggttccgt
     3061 cgaggtcgaa aatcacggcg cgatctggca tgcaaactcc gcaagtctgg tgggtttgtt
     3121 ccccgcaaac ccctgtgcta agaggccgcc caatcgcaac gggagaccaa acatggacat
     3181 ccgcaaggcc caggcgcttc gcgacatact gaaggacgcc gcgctcctca aggagaaatg
     3241 ttacatcgac ggcaagtggg tgggtggcaa gacaaccatc gacgtgacca acccggttga
     3301 cgagagcgtg atcgccacgg ttccgaaact tggcgctgcc gagacccgtg ccgccatcga
     3361 ggctgcccag cgcgcccaga aggactgggc gaagaagact gcgaaggaac gcgcggccat
     3421 cctgcgcaag tggttcaacc tcatgatgga gaaccaggag gatcttgccc agatcatgac
     3481 cgccgagcag ggcaagccgc tggccg
//
LOCUS       DBNGVR010000451         1747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4488629, whole genome shotgun sequence.
ACCESSION   DBNGVR010000451 DBNGVR010000000
VERSION     DBNGVR010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4488629"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="AC16A0_09600"
     CDS             <1..1108
                     /locus_tag="AC16A0_09600"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3105630.1"
                     /translation="PPAIYSDAEIAKLELARIFRTDWLCPGLAADIPNPGDYVTWNIA
                     GQPIYCVRGRDGGIRTFANVCLHRMMTLLQDRGNCQRISCPYHGWTYDIDGRVIGAGH
                     MQGRDPEFEKKGYRLPEIRTEIWEGWIYVTLNDEAPPVAELLRELEPEVSRYRLDKYV
                     SVALQDHLWNTNWKLLTENFMEGYHLPVAHRETVGAWFPVEATKFPEKVFDAFTWQTF
                     EKDETAKYGRAHPDNAHLEGRWRYTSLMPTVFPSHMYVLAPDHMWYLSLHPKDVDHVH
                     VRFGLAVAPEVCAGLGDKLPEWLEATLAFFDKVNAEDKFVVEGIHRGSSSPLARPGPF
                     SWLEREIHDFARYLDRRLSPPKKPALQEVGRRMA"
     gene            1115..>1747
                     /locus_tag="AC16A0_09605"
     CDS             1115..>1747
                     /locus_tag="AC16A0_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3105631.1"
                     /translation="MTAASTLLLTDERCFWHAGKSHALIFPVGGWVQPPSGSDYAESP
                     DSKRRLKSLLDVSGLTVQLDVSSAAMASDEDLRRVHPASYLARFAALSAGEGGEVGFE
                     ASFGPGGYDIARLSAGLAVEAVSRVAAGTLRNAYALTRPPGHQCLPDSGLGFCLLANI
                     AIAVERAIAEHKLMRVAVIDWDVHHGNGTEAIFYERPDVFTISLHQERCYP"
BASE COUNT          302 a          547 c          553 g          345 t
ORIGIN      
        1 tccgccggca atctactccg atgcggagat cgccaagctt gaactggcgc gaatctttcg
       61 gacggactgg ctatgtccag gcctcgccgc cgacattccg aatcccggtg attacgtgac
      121 ctggaacatc gccgggcagc ccatctactg tgtgcgtggc cgcgacggcg gcatccgaac
      181 cttcgccaat gtctgcctgc accggatgat gacgctgctg caggaccgcg gcaattgcca
      241 gcgcatttcc tgcccgtatc acggctggac ctatgacatc gatggtcgtg tcatcggggc
      301 cggacacatg cagggccgtg atccggagtt cgagaagaag ggctaccggc tgcccgaaat
      361 caggaccgag atctgggagg gctggatcta tgtcaccctc aatgacgagg ctccgcccgt
      421 ggcggaactg ctgcgcgagc tggaacccga agtctcacgc taccgtctcg acaaatatgt
      481 gtcggtcgcg ttgcaggacc atctgtggaa cacgaactgg aagctgctga ccgagaactt
      541 catggaggga tatcaccttc cagtcgccca tcgagagacg gttggcgcgt ggtttccggt
      601 ggaggccacc aagtttccgg agaaggtctt cgatgccttc acctggcaga ccttcgagaa
      661 ggacgagacc gcgaagtacg gccgcgccca tcccgacaac gcgcatctcg aaggccggtg
      721 gcgctacacc tcgctcatgc cgacggtgtt tcccagccac atgtacgtgc tggcgcccga
      781 ccacatgtgg tacctttcgc tccatccgaa ggacgttgac cacgttcatg tgcgttttgg
      841 gctggccgtc gcgcccgagg tctgtgcggg acttggcgac aagcttccgg agtggctcga
      901 agcaaccttg gccttcttcg acaaggtcaa tgccgaagac aagttcgtgg tggagggaat
      961 acaccggggt tcgtcctctc cgctggcaag gccaggacca ttcagttggc tcgagcgcga
     1021 gattcacgac tttgcccgct acctggatcg ccgtctttcg cctccgaaga aacctgcgct
     1081 tcaggaagtt ggccgccgca tggcgtgatg gcagatgacc gcggcctcaa ccctcttgct
     1141 caccgatgag cgctgtttct ggcatgcggg gaagtcccat gccctgatct ttccggtggg
     1201 cggctgggtg cagccgccga gtggctcgga ctatgcggag tcgcccgaca gcaagcgccg
     1261 gctcaagagc ctgctcgatg tctccggcct tacggtgcaa ctcgatgtca gctctgctgc
     1321 gatggcaagc gacgaggatc ttcgccgcgt gcatccggca tcctatctcg cgcgcttcgc
     1381 ggcgctcagc gcgggggagg gcggcgaggt cggcttcgag gcgtccttcg ggccgggggg
     1441 ctacgacatc gcgcggcttt cggccgggtt ggcggtggag gccgtttcgc gcgttgctgc
     1501 gggcacgctg cgcaatgcct atgcattgac gcggccgccc gggcatcagt gccttcccga
     1561 cagcggcctg ggcttctgcc ttctcgccaa catcgcgatt gccgtcgagc gcgccattgc
     1621 cgagcacaag ctgatgcgcg tggcagtgat cgactgggac gtgcatcacg gcaacggtac
     1681 cgaggcgatc ttctacgagc ggcctgacgt tttcaccatc tccctgcatc aggaacgctg
     1741 ttatccg
//
LOCUS       DBNGVR010000452         2553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1348071, whole genome shotgun sequence.
ACCESSION   DBNGVR010000452 DBNGVR010000000
VERSION     DBNGVR010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1348071"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..573
                     /locus_tag="AC16A0_09610"
     CDS             28..573
                     /locus_tag="AC16A0_09610"
                     /inference="COORDINATES: protein motif:HMM:NF040413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HIR3105632.1"
                     /translation="MSAYASASYSLATAMPVIDSLSKLRAALQPHAELPLVFIYDDRR
                     IAPGYHVTEVKAARLSSLDCGANPESWSETVIQLWDVNATAREHLKTAQFLGIMEQVE
                     RRVALDADGLLIFECGDSARPMQVFTVGHIRTADGHVTVELAARTATCKPRERGLDAG
                     EVPAASGCCSTPKPQAAACCS"
     gene            complement(579..1772)
                     /locus_tag="AC16A0_09615"
     CDS             complement(579..1772)
                     /locus_tag="AC16A0_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3105633.1"
                     /translation="MKVKDLKVFIVGNPPPSWGGRYFIFVKLTTDTGISGIGECYCDT
                     FGPKAMTAMIEDTFGRYVEGMDPFQLESLWRKVYSSGFSQRPDISLMGVLSGIEIALW
                     DIIGKALNKPVYELLGGKVHEKLRSYTYIYPDPAKGQDGSIFWNAEQSAERAAEYVRQ
                     GFTAVKFDPAAPYSAFDPRQPSLESIDLCVKFCKLIREAVGSKADLLFGTHGQFTASG
                     AIRFAKKIEPFDPLWFEEPTPPEKPEEMALVARGTSIPVATGERLTTKYEFARVLECR
                     SASILQMALGRVGGIMEAKKIAGMAEAYYAQIAPHLYAGPVEGAANIHFAASLPNFLI
                     LESIEQWGGFHSELLKKPIRWDNGYVILPTEPGLGIELNETVAEAHPYTGTNLHLEMT
                     NTPVR"
     gene            1921..>2553
                     /locus_tag="AC16A0_09620"
     CDS             1921..>2553
                     /locus_tag="AC16A0_09620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3105634.1"
                     /translation="MEFDFIIVGAGSAGCVLAERLTADGRHSVLVLEAGGSDRRFFVQ
                     MPLGYGKTFYDPRVNWMYQAEPDPGLNGQRDYYPRGKILGGSSSINAMVYIRGHAEDY
                     EDWKRAGNPGWGFDEVLPAYKAIEDYAAGDPAYRGRGGPLHISDIGDDAHPLCRNFIA
                     ASEAAGLAFNADFNGAEQEGVGLYQLTTKGGRRMSAARAFLRPAMRRRNLK"
BASE COUNT          436 a          796 c          873 g          448 t
ORIGIN      
        1 gaatattgaa atgaaggata gatcaacatg agcgcctatg ccagtgcaag ttactctctc
       61 gccacagcca tgccggtcat cgacagcctg tccaagctgc gggccgccct ccagccccat
      121 gccgagctgc cgctggtctt catctacgac gaccgcagga ttgcgccggg ctatcacgtg
      181 acggaggtga aggcggcgcg cctgtcgtcg ctcgactgcg gggccaatcc cgaaagctgg
      241 agcgagaccg tgatccagtt gtgggatgtg aacgcgacgg cgcgcgagca cctgaagacc
      301 gcccagttcc tggggatcat ggagcaggtg gagcggcgcg tggcgctgga tgcggacggc
      361 ctgctgattt tcgagtgcgg cgactctgcg cggccgatgc aggtcttcac cgtgggccac
      421 atccgcaccg ccgacgggca tgtcacggtc gaactcgccg ccaggaccgc cacctgcaag
      481 ccccgcgagc gcggtctcga cgcgggggag gtgcctgccg ccagcggctg ctgcagcacg
      541 ccgaaaccgc aggccgcagc gtgctgcagc tagcatcgtc agcgcaccgg cgtgttggtc
      601 atttcgaggt gaaggttggt gccggtgtag ggatgggctt ccgcgaccgt ctcgttcagt
      661 tcgatgccga ggcccggttc ggtgggcagg atcacgtagc cgttgtccca gcggatgggt
      721 ttcttcagca gctcggaatg gaagccgccc cattgctcga tcgattcgag gatcaggaaa
      781 ttgggcagcg aggccgcgaa gtggatgttg gccgctccct cgaccgggcc ggcgtagaga
      841 tgcggcgcga tctgggcgta gtaggcttcc gccatgccgg cgatcttctt ggcctccatg
      901 atgccgccga cgcggcccag cgccatctgc aggatggagg ctgagcggca ttccagcacg
      961 cgggcgaact cgtacttggt ggtgagccgc tcgccggtgg ccaccgggat ggaggtgccg
     1021 cgcgccacca gcgccatttc ctccggcttc tccggcggcg tcggctcctc gaaccacagc
     1081 ggatcgaagg gctcgatctt cttggcgaag cggatggcgc cagaggcggt gaactggcca
     1141 tgggtgccga agaggaggtc ggccttcgag cccaccgcct cgcggatcag cttgcagaac
     1201 ttcacgcaga gatcgatcga ctcgagcgaa ggctggcgcg gatcgaaggc ggaatagggg
     1261 gccgcaggat cgaacttcac ggcggtaaag ccctgccgca catactccgc cgcgcgctcg
     1321 gcagactgtt cggcgttcca gaagatcgag ccgtcctggc ccttggccgg atcgggatag
     1381 atgtaggtgt aggagcgcag cttctcgtgc accttgccgc cgagcagttc gtagaccggc
     1441 ttgttcagcg ccttgccgat gatgtcccac agcgcgatct cgatgccgga aagcacgccc
     1501 atgagcgaga tgtcggggcg ctgcgagaag ccggaggaat agaccttgcg ccacaggctc
     1561 tcgagctgga acgggtccat gccctcgacg tagcggccga aggtgtcctc gatcatcgcc
     1621 gtcatggcct tggggccgaa ggtgtcgcaa tagcattcgc cgatgccgct gatgccagtg
     1681 tcggtggtga gcttcacgaa gatgaagtag cggccgcccc aggagggcgg cggattgccg
     1741 acgatgaaga ctttgaggtc tttgactttc atgtttctgc cctcccggat tggcctgctc
     1801 ttaccagttc gcacggcggg cgcgcgatga aattttctaa ccacgcgtga gcggaacgcc
     1861 cttgtttcca aggcggcggt cgctgcataa cgagcccgat tcaccggggg gcggaaagcc
     1921 gtggagttcg atttcatcat agtgggcgcc ggttctgccg gctgcgtact ggccgagcgg
     1981 ctcacggcgg acgggcgcca cagcgtgctg gtgctggagg cgggcggcag cgaccgccgc
     2041 ttcttcgtgc agatgccctt gggctatggc aagaccttct acgacccccg cgtcaactgg
     2101 atgtaccagg ccgagcccga tcccggcctc aacggtcagc gcgactatta tccgcgcggc
     2161 aagatcctgg gcggctcctc ctcgatcaat gccatggtct atatccgcgg ccatgccgag
     2221 gactacgagg actggaagcg cgccggcaat cccggctggg gcttcgacga ggtgctgccc
     2281 gcctacaagg cgatcgagga ttatgccgcg ggcgatcccg cctatcgcgg acggggcggg
     2341 cctttgcaca tctccgacat cggcgacgac gcgcatccgc tgtgccgcaa cttcattgcc
     2401 gcgagcgaag cggcgggcct cgccttcaat gccgacttca acggcgcgga gcaggagggc
     2461 gtggggttgt atcagctcac caccaagggc ggccgccgca tgtcggcggc gcgcgccttc
     2521 ctgcgcccgg cgatgcggcg cagaaacctg aag
//
LOCUS       DBNGVR010000453         4121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3114634, whole genome shotgun sequence.
ACCESSION   DBNGVR010000453 DBNGVR010000000
VERSION     DBNGVR010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3114634"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="AC16A0_09625"
     CDS             <1..392
                     /locus_tag="AC16A0_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3105635.1"
                     /translation="AHGPQRRKVLQVAALAMGTCDKAVSLFFERYFHPGKSLSKDYEQ
                     RCLSQIGAALDRLEHDCGVPWFFDSHLSQADVSVGCMIGYLKIRVPDAFPAAKYPKLH
                     ALALHCETRDEFVKARPAPNESVPARS"
     gene            complement(389..1021)
                     /locus_tag="AC16A0_09630"
     CDS             complement(389..1021)
                     /locus_tag="AC16A0_09630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3105636.1"
                     /translation="MIDLHTWNTPNGKKISIALEEMGLAYTVRPVNIGKDEQFQPSFL
                     EISPNNKIPAIVDHETGDSVFESGAILMYLGDKSGLYYSTERKTRIRIHEWLMWQMSA
                     VGPMLGRAHYFLKYNPDKAPFAEAWFSAEALRIYGILDRLLARQPFMCGDYSIVDMAT
                     WPWIARHEWQRIELSRFPHVRRWYLEIAARPAVQRGYKVPDPSAEIPIPA"
     gene            complement(1018..1782)
                     /locus_tag="AC16A0_09635"
     CDS             complement(1018..1782)
                     /locus_tag="AC16A0_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3105637.1"
                     /translation="MIASLPMYDWPEVRDATDAWWHGLARHIGVAGELSRIEDYASLW
                     RDPDLILSQTCGYPFTHAFRNRLTLVATPHYGVDGCEAANYRSIVFARHGAVLESFRG
                     ATAAVNAPDSMSGMLALQLVFAPLARAGTFFGDVIWSGGHLASLAAVREGRADVCAID
                     AVCVGLARRYRPRDMEGLVEIARSPLVPGLPYVTNASDAETIRKGVLSAFADPSLQVV
                     RDHLFLSGVSMPGPSVYERILDLEARMKHAGGLKLP"
     gene            complement(1779..2624)
                     /locus_tag="AC16A0_09640"
     CDS             complement(1779..2624)
                     /locus_tag="AC16A0_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3105638.1"
                     /translation="MQKRDTVDVFRRRLTELIAVTGETRARFAARAGLDRSTLSQLLS
                     PQNVRLPRAETIATIAARNQASVDWLLGLSEVPQVTADIVSQPLVTQGADDPASDHLR
                     RWHEEARGAKVRYVPSTLPDQVKTAAVLHYENARLSRAQAEAMSATARARIEHLRQSS
                     SEIEVCSSRQSLELFARGEGLWRELPLRERRRQLDRMASEVDQLYPSYRWFLFDARET
                     YAAPYTVFGQKRAALYVGSMFFVFTSTDHVRELTAHFDNLIRQARVQPNETSAFIARL
                     ARDLA"
     gene            2735..3655
                     /locus_tag="AC16A0_09645"
     CDS             2735..3655
                     /locus_tag="AC16A0_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR3105639.1"
                     /translation="MPTDRKRAIEWPTVFLIMLTYLTVGLLVWNHERLPWWVLLPVGG
                     YFAALYTSLQHEVLHGHPTSNRSLNEALIFPTLHFWLPYGRYRDTHLRHHNDTDLTDP
                     RLDPESYYMLPDDWARLPGLKRSLYQFNQTLGGRMLIGPAVSIVRFWTSEFRDIAHGN
                     RETLRAWTIFAMSCAITIWFVTVIAGMPLWQFILLIAYPGISLSLVRSYCEHRAAEHQ
                     DHRTIIVEASPFWSLLFLNNNLHVAHHNKPALAWYKLPAYYAAERAELIARNDGYMMQ
                     GYGEIFRRYFFTAKEPIPYPDMRWLKKDVT"
     gene            3687..>4121
                     /locus_tag="AC16A0_09650"
     CDS             3687..>4121
                     /locus_tag="AC16A0_09650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3105640.1"
                     /translation="MVSFSVVIPTRNRTAFLRQAIASVLAQTHGAMEIIVVDDGAGAA
                     QALKDTSPLLRVFDNRERGPVPARNLGVAQAQGDVVAFLDDDDWWTDRDYLSLASQAF
                     ENSAALCFGDGIMAFEDGREQASFAFDADAISLERDNTILISA"
BASE COUNT          759 a         1323 c         1297 g          742 t
ORIGIN      
        1 ctgcacatgg accgcagcgg cgcaaggtgc tgcaggtggc ggccctggcc atgggcacct
       61 gcgacaaggc cgtgagcctt ttcttcgagc gctatttcca tcccggcaag tcgctgtcga
      121 aggattatga gcagcgctgc ctgtcgcaga ttggcgccgc cctcgatcgc ctcgaacatg
      181 actgcggcgt gccatggttc ttcgacagtc acctgagcca ggcggacgtc tcggttggct
      241 gcatgatcgg ttacctcaag atccgggtgc cggacgcctt tccggccgcg aagtatccaa
      301 agctgcatgc gctggcgctg cattgcgaga cccgcgacga gttcgtcaag gcgcggcctg
      361 ctccaaacga aagcgttccc gcgcgcagct aggccggaat cggaatctcc gccgaaggat
      421 cgggcacctt gtagccgcgc tgcacagccg gccgcgcggc gatttccagg taccagcgcc
      481 tgacatgcgg aaagcgcgac agctcgatgc gttgccactc atggcgtgcg atccagggcc
      541 aggtggccat gtcgacgatg gaatagtcgc cgcacatgaa aggctgccgg gcgagaagcc
      601 ggtcgagaat gccgtagatg cggagtgcct cggccgagaa ccacgcttcg gcgaaaggag
      661 ccttgtccgg attgtacttc agaaaatagt gcgcccggcc cagcatgggt ccgaccgccg
      721 acatctgcca catcagccac tcgtgaatcc tgatgcgggt cttgcgttct gtggaatagt
      781 acaagccgct cttgtcgccc agatacatga ggatggcacc ggattcgaag acactgtcgc
      841 cagtctcgtg atcgacgatg gcaggaatct tgttgttggg cgagatctcg aggaaggacg
      901 gctggaactg ttcatccttg ccgatgttga cggggcggac cgtgtaggca aggcccatct
      961 cttccagcgc gatcgagatc ttcttgccgt tgggcgtgtt ccaggtgtgc aggtcgatca
     1021 tggcagcttc aatcctccgg catgcttcat ccgggcctcg agatcgagga tccgctcgta
     1081 gactgacggt cctggcatcg agacgccaga caagaagagg tgatcgcgca caacctgaag
     1141 cgatggatcg gcaaatgccg agagtacgcc ctttcggatc gtttcggcat cggaggcatt
     1201 ggtcacgtag ggcaggcccg gcacaagcgg agagcgggcg atttcgacca ggccttccat
     1261 gtcgcgcggc cggtagcgcc gggcaagccc gacgcagacg gcatcgatgg cgcagacatc
     1321 ggccctgccc tcgcggacag cggccagcga cgcgagatgg ccgccgctcc agatgacatc
     1381 gccgaagaat gttccagcgc gcgccagcgg cgcgaagacg agctgcagcg ccagcattcc
     1441 ggacatcgag tcaggcgcgt tgaccgcggc cgtggcgccg cggaagctct cgaggacggc
     1501 gccgtgccgc gcaaacacaa tgctgcggta gttggccgct tcgcaaccat cgacgccgta
     1561 gtgcggcgtt gcgaccagcg tcagccggtt cctgaacgcg tgggtgaagg gatagccgca
     1621 ggtctggctc aggatcagat cagggtcgcg ccagaggctg gcatagtcct cgatccggga
     1681 caattcaccc gcgactccga tgtgccgcgc gaggccatgc caccaggcat cggtggcatc
     1741 gcggacttca ggccagtcat acatgggcaa ggaggcgatc atgcgaggtc ccgggcgagt
     1801 ctggcgatga aggctgaggt ctcgttgggc tggaccctgg cctggcgaat gaggttgtcg
     1861 aaatgcgccg tcagttcacg cacatggtcg gtcgaggtga agacgaagaa catcgacccg
     1921 acatagagcg ccgcgcgctt ctggccgaag acggtgtagg gcgcggcata ggtctcgcgg
     1981 gcgtcgaaga ggaaccagcg ataggacgga tagagctggt caacctcgct tgccatgcgg
     2041 tcaagctggc gccgccgttc gcgcagcggc agttcccgcc agagaccttc gccgcgcgcg
     2101 aagagttcca gagactgccg cgacgagcag acctcgattt cgctggacga ctggcgcaga
     2161 tgctcgatgc gggcgcgcgc cgtggccgac atggcctcgg cctgtgcccg cgacagccgg
     2221 gcgttttcgt agtgaagcac agctgcagtc ttcacctggt ccggcagggt cgacggcacg
     2281 tatcggacct tcgcaccacg ggcctcttcg tgccagcgcc ggaggtgatc gctggccggg
     2341 tcgtccgcac cctgtgtcac cagtggctgc gagacgatgt cggcggtgac ctggggtacc
     2401 tcggaaagac cgagaagcca gtccaccgaa gcctggttcc gcgccgcgat ggtggcgatg
     2461 gtctcggctc ttggcagccg cacattctgg ggtgacagca gttgcgaaag cgtcgagcgg
     2521 tcgagccccg cccgggcggc gaagcgggcg cgggtctcgc ctgtcacggc aatgagctcg
     2581 gtcagccggc ggcgaaagac gtccacggtg tcgcgcttct gcatggcagc cttgtttaca
     2641 gttgtcacca ctatatacat ttgtgtatca tcatcaacag acttgcccgg cgaaccgggc
     2701 taaggctgtg cgatcctccg gggccgaacc gaccatgccg accgaccgca agcgcgccat
     2761 cgaatggccg accgtgttcc tgatcatgct gacctacctg acggtgggcc tgctggtctg
     2821 gaaccatgag cgcctgccct ggtgggtcct cctgcccgtc ggcggctact tcgcggccct
     2881 ctacacgtca ctccagcacg aggtgctgca tggccacccg acatcaaatc gcagcctgaa
     2941 cgaggcgctg atcttcccga cgctgcattt ctggctgccc tacggacgct accgcgacac
     3001 ccatctgcgc caccacaatg ataccgacct gaccgatcct cgcctggacc ccgagtccta
     3061 ctacatgctg ccggacgact gggcacggct gcccggcctc aaacggtcgc tgtaccagtt
     3121 caaccagacg ctgggcggac gcatgctgat cggccccgcc gtgagcattg tccgtttctg
     3181 gacctcggag ttccgcgaca tcgcgcatgg taaccgcgag acgctgcgcg cctggaccat
     3241 cttcgctatg tcttgcgcga tcaccatctg gttcgtcacc gtcatcgcgg gcatgccgct
     3301 gtggcagttc atcctgctca ttgcctatcc cggcatctcc ctgtcactgg tgcgctccta
     3361 ttgcgaacat cgggccgcgg aacaccagga ccatcgcacc atcatcgtcg aggcctcgcc
     3421 cttctggtcg ctgctcttcc tcaacaacaa cctgcatgtc gcgcatcaca acaagccggc
     3481 gctcgcctgg tacaagctgc cggcctatta tgccgccgaa cgcgccgaac tcatcgcccg
     3541 caacgatgga tacatgatgc aaggctatgg cgagatcttc cgccgctact tcttcacggc
     3601 gaaggaaccg atcccctatc ctgacatgcg ctggctgaaa aaggacgtga cctgatctcg
     3661 ggcacggatg ctatcgtagc gccaccatgg tcagcttctc cgtcgtcatc cccacccgca
     3721 accggacagc cttcctcagg caagccattg ccagcgtgct tgcgcagact catggagcaa
     3781 tggagatcat cgtcgtcgac gatggcgccg gcgcagcaca ggcgctgaaa gacacatcgc
     3841 ctctcctccg cgtgttcgac aatcgcgaaa gaggccccgt gcccgcgcgc aacctgggcg
     3901 tcgctcaggc gcaaggcgat gtggtcgcct ttctcgatga cgacgactgg tggaccgacc
     3961 gggattacct ctcgctcgcg tcacaggcct tcgagaacag tgccgcgctc tgcttcggcg
     4021 acggcatcat ggccttcgag gacggccggg agcaggcgag cttcgccttc gatgccgacg
     4081 cgatatcgct ggaacgggac aataccatcc tgatctctgc g
//
LOCUS       DBNGVR010000454         2267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4490725, whole genome shotgun sequence.
ACCESSION   DBNGVR010000454 DBNGVR010000000
VERSION     DBNGVR010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4490725"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="AC16A0_09655"
     CDS             complement(<1..660)
                     /locus_tag="AC16A0_09655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3105641.1"
                     /translation="MSYDHPEFAGSKLHAKVLRPKDSATLILVRRDGPKPRILMGQRS
                     GGHAFMPNKYVFPGGRLDPADCRVKPARDLDPATLAKLMARMRGRATPSRARGLAHAA
                     LRETWEEAGLVVGRSHIEEAPDLSRLVLFMRAITPPGRTQRYDSRFFVADAETVSNLD
                     RPHHDGGGELLTLSWLTLDDIRGLDLPLVTVDVLDRLKPHLDQGRLPAPETPISFQYY
                     RG"
     gene            complement(657..1076)
                     /locus_tag="AC16A0_09660"
     CDS             complement(657..1076)
                     /locus_tag="AC16A0_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HIR3105642.1"
                     /translation="MTVTIDDPSAAPLEVRSWKQAMIRGARCRCPACGAGLMFAKFLK
                     VSDSCQACGEELHHHQADDAPPYFTMFIVGHIVVPLVLIVEKLWHPALPIHFVVWTIV
                     TLALSFAIMPAVKGAIVGLQWALRMHGFELPARRGTQ"
     gene            complement(1146..>2267)
                     /locus_tag="AC16A0_09665"
     CDS             complement(1146..>2267)
                     /locus_tag="AC16A0_09665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R family protein"
                     /protein_id="HIR3105643.1"
                     /translation="RVIMRSAAKLSYEEAQAAIDGRGNDKASVLLDGVLKPLWRAYGI
                     LGKGRDSRGPLELDLPERKLVLNAHGLIERVVTPLRLDAHRLIEEFMIQANVAAAEEL
                     ESKKTPLLYRVHEPPSDEKLRALSEFLRTVNMSLPLGQVMRPKHFNRILAEVQGKDYQ
                     HVVNEVVLRTQSQAVYGPENKGHFGLALRRYAHFTSPIRRYADLIVHRALISALGFGD
                     DGLSPDDIAKLGETAEMISDAERRAMAAERETVDRLVAAHLAKQTGAVFRGRIGGVVS
                     AGLFVTLDDSGADGFVPVSTLGAGYYVHDRTRHALIGERSGETYQLGDRVEVKLVEAT
                     PVSGGMRFEVVSEGRAGQPMPRRAVRTSRQTSRKGPRRR"
BASE COUNT          376 a          734 c          800 g          357 t
ORIGIN      
        1 gccgcggtag tactggaacg agatgggggt ctccggggcg gggaggcggc cctggtcgag
       61 gtggggtttc aaccggtcga gcacatccac cgtgaccagc ggcaggtcga ggccgcggat
      121 gtcgtccagc gtcagccacg acagggtcag cagctcgccg ccgccatcgt ggtgcggccg
      181 gtcgaggttg gacacggtct cggcatcggc cacgaagaaa cgggaatcat agcgctgcgt
      241 gcggcccggc ggggtgatgg cccgcatgaa cagcaccagg cgcgacagat cgggcgcctc
      301 ctcgatgtgg ctgcggccga ccacgaggcc ggcctcttcc caggtttcgc gcagcgcggc
      361 atgggcgagg ccgcgcgccc gcgacggggt cgcacggccg cgcatccggg ccatgagctt
      421 ggccagcgtg gcgggatcga gatcgcgggc gggcttgacc ctgcagtcgg ccggatcgag
      481 gcggcccccg gggaagacat acttgttcgg catgaaggca tggccgccgc tgcgctggcc
      541 catgaggatg cggggcttcg gaccgtcgcg ccgcaccagg atgagggttg ccgaatcctt
      601 cggccgcagc accttcgcat gaagcttgga gccggcgaat tccgggtggt cgtagctcat
      661 tgcgtcccgc gccgggccgg caattcgaag ccatgcatgc gcagcgccca ttgcaggccg
      721 acgatggccc ccttcacggc cggcatgatg gcaaagctga gcgccagggt gacgatggtc
      781 cagaccacga aatggatcgg cagggccgga tgccagagct tttccacgat caggaccagc
      841 ggcaccacga tgtgtccgac gatgaacatg gtgaagtaag gcggcgcgtc atcggcctga
      901 tgatggtgca gttcttcgcc gcaggcctgg cagctgtccg agaccttgag aaacttggcg
      961 aacatcagtc cggcgccgca ggccgggcag cggcagcggg cgccgcggat catggcctgc
     1021 ttccaggagc ggacttccag cggggcggcg gatggatcgt cgatggtcac ggtcatggcc
     1081 gcaccatgcg caagtgctgc ggcagtttga aggcccgggc ggtcggcgcg acggagtgcc
     1141 gcaagtcagc gccgccgcgg tcccttgcgc gatgtctgcc ggctggtccg caccgcccgc
     1201 cgcggcattg gctggcccgc gcgcccctcg gagacgactt cgaagcgcat gccgcccgaa
     1261 acgggggtgg cttccaccag tttcacctcg acgcggtcgc ccagctgata ggtctcgccg
     1321 ctccgctcgc cgatcagcgc atggcgggtg cggtcatgga catagtagcc cgcccccagc
     1381 gtggacacag gcacgaagcc gtcggccccc gaatcgtcca gcgtcacgaa gaggccagcg
     1441 ctcaccacgc cgccaatccg tccgcggaat accgcaccgg tctgcttggc cagatgcgcg
     1501 gcgacgagcc ggtccacggt ctcgcgttcg gccgccatgg cacggcgctc ggcatccgag
     1561 atcatctctg ccgtctcgcc cagtttcgcg atgtcatcgg gcgaaagtcc gtcatcgccg
     1621 aagccaagcg ccgagatcag cgcgcggtgc acgatcaggt cggcatagcg gcggatcggc
     1681 gaggtgaaat gcgcgtagcg gcgcagcgcc aggccgaaat ggcccttgtt ctccggccca
     1741 tagaccgcct gcgactgggt gcggagaacc acctcgttca ccacgtgctg atagtccttg
     1801 ccctgaactt ccgccaggat gcggttgaag tgcttcggcc gcatgacctg gcccagcggc
     1861 agcgacatgt tcacggtacg gaggaattcc gacagtgcac ggagcttctc gtccgagggc
     1921 ggttcatgga cacggtagag caggggcgtc ttcttcgact ccagttcctc ggcggcggca
     1981 acattggcct ggatcatgaa ttcctcgatc agccggtgcg cgtcgaggcg gagcggggtc
     2041 accacccgtt cgatcagccc atgggcattg agaacgagct tgcgttcggg aagatcgagt
     2101 tccaggggtc cgcgcgagtc gcggcccttg ccgagaatgc cataggcgcg ccagagcggc
     2161 ttcagcacgc cgtcaagcag gaccgaggcc ttgtcgttgc cgcgtccgtc gatggcagcc
     2221 tgagcctcct cgtaggagag tttggcggcc gagcgcatga tgacgcg
//
LOCUS       DBNGVR010000455         4824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3509551, whole genome shotgun sequence.
ACCESSION   DBNGVR010000455 DBNGVR010000000
VERSION     DBNGVR010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3509551"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..578
                     /locus_tag="AC16A0_09670"
     CDS             228..578
                     /locus_tag="AC16A0_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR3105644.1"
                     /translation="MAAAATLTEHPLPLLIAVATIGNVLGSCLNWLLGRLVSAGYVLR
                     LPVSGARLGRFEAFFSRFGVWSLLLSWVPIVGDPLTVLAGLARTPLILFVPLVTIGKL
                     ARYLAVAGLVGLGG"
     gene            646..2025
                     /gene="mgtE"
                     /locus_tag="AC16A0_09675"
     CDS             646..2025
                     /gene="mgtE"
                     /locus_tag="AC16A0_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3105645.1"
                     /translation="MADGLTESVRDDRGAFDPDFVARVTQALELGDGKLARSLTRDLH
                     AADLAELIGNLDQDERVRLIALLGRNFDVEALAELDVAARDELMEALPNEVIASAIKK
                     LDTDDALYLIEDLEDDDQREILAKVSKQDRAALTRALDYPEYTTGRLMKTEFVAVPSS
                     WTVGRAIDYIRTDKSLPDNFIEVYVIDTGFHLVGTVAVARLLRSPRDRKIEAIMDEEQ
                     TVFKVTDPQDDAAYRFEKYNLVSAAVTDEIGRLVGTLMADDIVEVIQEEAGEDILHLG
                     GVGEETISDNVWETTRSRFLWLFVNLATAVLASWVISWFDATLEQMVSLAILMPIVAS
                     MGGNAGTQTMTVAVRALATRNLGPMNAVKVTLRECAVGLINGLVFAMIIGAFAWWWFS
                     SDGLGLVIAAAMVINLVAAALAGILVPLALHRMKVDPAIASGVFVTTVTDVIGFFAFL
                     GLAALWLVR"
     gene            2094..2468
                     /locus_tag="AC16A0_09680"
     CDS             2094..2468
                     /locus_tag="AC16A0_09680"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3105646.1"
                     /translation="MGGRVPEDVSASAWTQIITPGSGLFALLAFSNEGALGFANVSAT
                     PFAWTAGPILFLQDLYVAPEARGRGLGRSLLQAVYDEADALGAAQVFWMVDELDSDLQ
                     AFYARQGIRTPYLRYMRRPWSW"
     gene            complement(2469..2957)
                     /locus_tag="AC16A0_09685"
     CDS             complement(2469..2957)
                     /locus_tag="AC16A0_09685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3105647.1"
                     /translation="MIHGNPPNTIPWPPIIYLIAAASAVFLHQAMPLPWLPDGALRII
                     AAGVGLCLICAAIALDITAMLAFRRHRTTILPHRSATALITEGPFARSRNPIYLGNTM
                     LVFGTGLLLGIGWLLPAALIAAAVVQKLAIAREERHLARSFGRAWTEYAARTPRWLFF
                     KD"
     gene            complement(2954..4168)
                     /locus_tag="AC16A0_09690"
     CDS             complement(2954..4168)
                     /locus_tag="AC16A0_09690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3105648.1"
                     /translation="MTPTSENREKTDWLAIAILFLAGLAAAMHFAKVAPVMGEVGSDL
                     GLTLVAAGFAVSLLGIVGVVFAIAMGALVAVIGLGRGILIALFGGALVAAAGAIAPDS
                     STFLASRFLEGFSHLLIVVCAPALMAMHAAPRDRPVVLSLWGCFFGVGFAATSAAAPM
                     IITWGGWRGLMLAHALLLALIGLALRMTFKRSGHADERRPRPTLANLVSAHGDVYRSG
                     PPLLLALTFCCYTVLFLASLTFLGKYLTDALGWSAAAAGSGMAVASLISMVFTLMAGF
                     LIRYGLPVFLGFTGAFLSLAVSAAILFTLGPSEGVAMFLIAVMMAAFGLLPGFTFASV
                     PAIAPTPALAALTYGAIAQFGNVGTFVGTPIFAALYGRMGWTGGALFCFIVCLAGIAC
                     AALLLSAMKARQ"
     gene            4335..4748
                     /gene="rpoZ"
                     /locus_tag="AC16A0_09695"
     CDS             4335..4748
                     /gene="rpoZ"
                     /locus_tag="AC16A0_09695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3105649.1"
                     /translation="MARVTVEDCIDKVPNRFELVLLAGHRARMVSQGAPITVDRDNDK
                     NPVVALREIADETIVVDDLREEVIHSMQMHVEVDEPEPTEVPLIQQSGDMVMVNDQVE
                     GADMPEMEQMTEEELLRGLEGMPPAESPGSQRNGY"
BASE COUNT          901 a         1462 c         1601 g          860 t
ORIGIN      
        1 tgcccaagcc gaagccacgc cccgagcgcc gtcccaaccc gttccagagc gggtctctgg
       61 cgacagccgc gcccgccgaa cggacacgca caccggtctg attgcgactc cttgccttga
      121 tcacgcaccc ctgacgccgc tagaggcccg tcatgggttc acttctcggc ctcttcgcgt
      181 ccgcatttct ttcagccaca ttgctgcctg gcagttccga ggcagccttg gcggctgcag
      241 ccaccctcac cgaacatccc ctaccattgc tgatcgcggt cgccacgatc ggcaatgtgc
      301 ttggctcctg cctgaactgg ctgttgggac gccttgtttc agccggatac gtgctccggc
      361 tcccggtgag cggtgcccgg cttgggcgct ttgaggcgtt cttttctcgc ttcggcgtct
      421 ggagcctgct tctgtcctgg gtgccgattg tgggcgatcc gctgacggtt ctagccggac
      481 tggcccgtac gccgctgata ctctttgttc cgctcgtgac catcggcaaa ctggcgcgct
      541 atctcgccgt tgccggactg gttggactcg gcggctgagt ggttccgact tgcgccggag
      601 gacgaaatcc tcttgaggag gacaggcatt gagctgcggg tggtcatggc ggacgggctg
      661 accgaatcgg tgagagacga caggggggct ttcgacccgg actttgtcgc gcgcgtcacc
      721 caggccctcg aattgggaga tggcaagctt gcccgcagcc tgacccgcga ccttcatgct
      781 gcagatcttg cggaactgat cggcaatctt gaccaggacg aacgggtgcg gctgatcgcg
      841 ctgctgggca ggaatttcga cgtcgaagct ttggccgagc tcgatgtcgc cgctcgcgac
      901 gagttgatgg aggcgctgcc caatgaggtg attgcctcgg ccatcaagaa gctcgatacc
      961 gacgatgcgc tctatctgat cgaggacctg gaggacgacg accagcgcga gatcctggcc
     1021 aaggtctcga agcaggaccg ggccgcgttg acccgggcgc tggattatcc cgagtacaca
     1081 accggtcggc tgatgaagac cgagttcgtt gcagttccct cgtcctggac cgtggggcga
     1141 gccatcgact acatccgcac cgacaaatcg ctgccggaca atttcatcga agtctatgtg
     1201 atcgacaccg gattccatct ggtgggcaca gttgccgtgg cccggctgct gcggtcgccg
     1261 cgcgaccgca agatcgaagc gatcatggat gaggagcaga cggtcttcaa agtgaccgac
     1321 ccgcaggatg atgcggccta ccgattcgag aaatataatt tggtgtcggc tgcagtaacc
     1381 gacgagatag gccggctggt cggcacgctg atggccgacg acatcgtgga agtgatccag
     1441 gaggaggccg gggaggatat ccttcatctg ggtggcgttg gcgaggagac catcagcgac
     1501 aatgtctggg agaccacacg gtcgcgcttc ctttggctct tcgtcaatct ggccacagcc
     1561 gtcctggcat cctgggtcat ctcctggttc gatgcgacgc tcgagcagat ggtttcgctg
     1621 gcgatcctca tgccgatcgt ggcctccatg ggcggcaatg ccggaaccca gaccatgacc
     1681 gttgcagtgc gggcgctggc aacccgcaat ctcgggccga tgaacgctgt gaaggtcacc
     1741 ctgcgcgaat gcgccgtcgg actgatcaac ggactggtct ttgccatgat catcggcgcc
     1801 ttcgcctggt ggtggttctc ctccgatggc ctcgggcttg tgattgccgc cgccatggtg
     1861 atcaatctgg tcgccgccgc gctggctggc atcctggtgc cgctggccct gcaccggatg
     1921 aaggtggatc cggccattgc atcgggtgtc ttcgtcacca cggtgacgga tgtcatcggc
     1981 ttcttcgcct tcctcggatt ggcagcgctg tggctggtgc gctgactatt cgccgcgctg
     2041 tggctggtga cgaacaggcc tggtcgggcc tttggaccgc ttggcaatcc cacatgggcg
     2101 gcagggtccc ggaggatgtc tcggccagtg cgtggacgca gatcatcacg cctggctctg
     2161 gccttttcgc gcttctggcc ttttcgaacg aaggggctct tggcttcgcc aatgtcagtg
     2221 caacgccttt cgcctggact gccggaccga tcctgttcct ccaggatctc tatgtcgctc
     2281 cggaggcgcg gggcaggggg ctgggccgtt ccctgttgca ggccgtctac gatgaagccg
     2341 atgccctcgg cgccgctcag gtcttctgga tggtcgatga gctggattcg gacctgcagg
     2401 ccttctatgc gcggcagggc attcgcacgc cttatctgcg ctacatgcgg cggccctggt
     2461 cctggtgatc agtccttgaa gaagagccag cgcggcgtgc gtgcggcata ctccgtccag
     2521 gcgcggccga aggaccgggc gaggtggcgc tcttcgcgcg cgatggcaag tttctggacc
     2581 accgcggcgg cgatgagggc cgcgggcaga agccagccaa tgccaagcag caggccggtg
     2641 ccgaagacca gcatggtgtt gcccagatag atggggttcc gcgatctggc gaagggacct
     2701 tctgtaatca atgcggtggc actgcggtgt ggcaggatgg tggtacgatg acgccggaag
     2761 gccagcatgg cggtgatgtc gagtgcgatt gcagcacaaa tcaagcacag gccgacgccg
     2821 gcagcgatga tgcggagggc gccgtccgga agccacggca gtggcatggc ctgatgcagg
     2881 aacacggcgg aggcggccgc gatcaggtag atgatgggcg gccaggggat cgtgttgggc
     2941 gggttgccgt ggatcattgt cgtgccttca tcgcggagag gagcagtgcg gcgcatgcga
     3001 tgcctgcgag gcaaacaatg aagcagaaga gggctcctcc ggtccagccc atcctgccat
     3061 agagggcagc aaagatgggc gttcccacga atgtcccgac attgccgaac tgggcgatgg
     3121 cgccataggt gagtgcggca agtgccggcg ttggcgcaat cgcgggcacg ctggcgaagg
     3181 tgaagccggg cagcagacca aaggccgcca tcatgaccgc gatgagaaac atggccacac
     3241 cctcgctcgg gccgagggtg aaaagaatgg cggcgctcac ggccaatgac aggaaggcgc
     3301 ccgtgaagcc gaggaagacc ggcaagccgt accggatcag gaaaccggcc atcaaggtga
     3361 aaaccatgga tatcaaggac gccaccgcca tacccgaacc cgcggccgct gcgctccagc
     3421 ccagtgcatc cgtgaggtac ttgccgagaa aggtgagcga ggcgagaaac agcacggtgt
     3481 agcagcagaa ggtcaacgcg agcaggagcg gcggaccgct gcgatagacg tcgccgtgtg
     3541 ccgaaacaag gttcgcgagc gtaggacgtg ggcgccgttc gtcggcatgg ccggagcgct
     3601 tgaatgtcat ccggagagcc aggccgatca gggcaaggag cagggcatgc gccagcatca
     3661 ggccgcgcca gccgccccac gtaatgatca tcggagcagc agcgctggtc gcggcgaagc
     3721 cgacgccgaa gaagcagccc cagagcgaaa gaaccactgg ccggtcacgc ggtgcggcgt
     3781 gcatggccat aagggcaggt gcgcagacca cgatgagaag gtgcgagaag ccttccagga
     3841 accggcttgc caggaaggtg ctactgtcgg gcgcgatggc gcctgctgcc gccaccagcg
     3901 cgcccccgaa gagggcgatc aggatgccgc ggcccagtcc gatgacggca accagggcgc
     3961 ccatggcaat ggcaaagacc acgccgacga tgcccagcag cgacaccgcg aagcccgccg
     4021 ccacgagggt caggcccagg tcactgccca cctctcccat gacgggggcg accttggcga
     4081 aatgcatggc ggcggcaaga cccgccagaa agaggatggc gatggccaac cagtcggttt
     4141 tttcacgatt ttctgaagtt ggtgtcacgg tttggtgctc attcgggcca tccgtgtgag
     4201 catgggttcg gccgttctgt caaacccggc gccgccacac cctgccgcct ggaagcatgg
     4261 gcttgatttg gccgccattt ccgcctagaa ggccgggatt ccatcgaaca ccaaacaagg
     4321 agcagacgag ccccatggcg cgcgtcaccg ttgaagactg catcgacaag gtgcccaatc
     4381 gtttcgaact ggtcctcctc gccggccacc gcgcccgtat ggtgtcgcag ggtgcgccga
     4441 tcacggtgga ccgtgacaat gacaagaacc ccgtcgtggc gcttcgcgaa atcgccgacg
     4501 agacgatcgt cgtcgacgat ctgcgcgagg aagtgattca ctcgatgcag atgcacgtcg
     4561 aggtggacga accggaaccc accgaggttc cgctcatcca gcagtccggc gacatggtca
     4621 tggtgaatga ccaggtcgag ggcgccgaca tgccggaaat ggagcagatg accgaagagg
     4681 aactgctgcg cggcctggag ggcatgcccc cagcagaaag ccccgggagc cagcgcaacg
     4741 gctactagcc ctcagctcca gtaacgattt tcaggccgga cccctcgcgt ccggcctttt
     4801 ttgtcgtaca gtaacatcat gatg
//
LOCUS       DBNGVR010000456         5752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4099162, whole genome shotgun sequence.
ACCESSION   DBNGVR010000456 DBNGVR010000000
VERSION     DBNGVR010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4099162"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="AC16A0_09700"
     CDS             <1..505
                     /locus_tag="AC16A0_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3105650.1"
                     /translation="GFQLFRLYRESSGRTVKRPAEDEDPMLRLALSGLNDTQISRLMK
                     AAEVKECVSGDVLTRQKKPVNSLYFLYAGRVNVEVDGKTVAALDNGAFIGEIAYLTGK
                     PATATVVVDKPSRVLTFSRARLNKVIEEDPQISGIIYELLGRDLARKMGQVNSQRTAS
                     DLQTARI"
     gene            617..1312
                     /locus_tag="AC16A0_09705"
     CDS             617..1312
                     /locus_tag="AC16A0_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3105651.1"
                     /translation="MDYFAPSNEWFGHLAYACLAASYFMTNIFWLRVLAVVSMLLELA
                     YFNYAGGDLTTGMSWNAVFVAINLYQLFWLLRDRLTLKLPEHDGPVLRSALTGLDDAQ
                     IARLLKAAHWKSAAKGKTLTTQHKPVDELYFICSGRLSVQVDGSVMAHLEKGSFVGEI
                     AYLTGNPATATVVVEEPCRILAFSRTQLAKVVASDTHISGLIYQMLGRDLAMKMGQAN
                     RRHTAGPNNLAQV"
     gene            complement(1349..3559)
                     /locus_tag="AC16A0_09710"
     CDS             complement(1349..3559)
                     /locus_tag="AC16A0_09710"
                     /inference="COORDINATES: protein motif:HMM:NF021603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HIR3105652.1"
                     /translation="MGSRPHGRISAGHFRQLTAKLKRRESEPPILAALRAGEIAIPDL
                     LEPPAEAPAVPVEDEIAAAPPEVDIAAPADADIVVPDMPDLAPVPEMEARLPEHAADT
                     PESPVEPASFSLPQFPEIPHFRPDQPAFPAFEPTQVFAQPEPPAEALATDLRDEPDPF
                     GRVEDDGVIAEAAQSEQPEAAYADAPVQVEAAEVVAEESSDAIFPSLVESPAEPEPAL
                     EAIAEEVLQPVAGEAVHEPAAEAAVAPEEVIEELPPPVPEPVAPKPEHQIPTVLPAVQ
                     VAESTKPVSPIADRVFEAMIKTIADSVYAKPTAAERNAFLRELAEMAESEPEVPAEAQ
                     PESPVSPAVPPSAPVLAPVIEAVPIAPPPPPSVLLKPKPEPDPFAVTFKALAEPKPTE
                     TAEADEESGELALSLLEMMSMGAASGLPQERALAADTLLRILPRIPVKQLLAVVERVA
                     IMETPPALLVAKLIRDPRPEVVAPLLERCMHITDQDLITAANEADAGKRRMIARRRVI
                     SPVLADRLIALADPSVVLTLVRNPGASFSHDAFYVLGEVASEHHAILAPLVTRPDLPP
                     PVAFELFWFVPPELRRYILSRFLTDSETLNKILRITLATGGGDIPPADTRFPPRDTIE
                     AALSLVTEFRLDEGAQVLAEAAGISRDTALRIFADREGEPMAVILKALGYPRGQLSEA
                     MLKLGSGENAIIRPGRVIEELQNVFDSLSFNKARILLTYWDWYVRKSGPYAPRN"
     gene            3838..4239
                     /gene="msrB"
                     /locus_tag="AC16A0_09715"
     CDS             3838..4239
                     /gene="msrB"
                     /locus_tag="AC16A0_09715"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3105653.1"
                     /translation="MTTKIDKTDADWRKSLSPEAYRVLREHGTERAFTSPLNDEKRTG
                     MFTCAGCGKELFASDAKFESGTGWPSFYQPACGDAVGTTEDRSWFMRRTEVHCADCGG
                     HLGHVFPDGPKPTGLRYSINGVSLGFRPDKD"
     gene            4244..>5752
                     /locus_tag="AC16A0_09720"
     CDS             4244..>5752
                     /locus_tag="AC16A0_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3105654.1"
                     /translation="MTKTIPMPPRAEKQPRVETWHGHEKRDDYHWLKAENWQQVMQDP
                     AVLPSNIRAFLEGENAYHEAIMADTAALQEKLFAEMKGRIKEDDSSVPAPDGPWSYYS
                     SFVTGGQHPRYLRKARDLTGPEQMILDGNALAKGREYFRFGGIAMSPDQRLAAWSFDG
                     NGSEFYELKVRDVAAGSDLPDLLQNTAGGATWAADGKTFFYTLQDENHRPLKTFRHRL
                     GKAQDADVLVYEERDTGMFTGVGKTNSDAYIVIDVHDHDTSEIRIIDAKAPEAEPRLI
                     APRQVGVEYDLEHWNDRFVIKTNIGGAEDYKIVTAPVANPDPENWTDLIPHRPGIFLV
                     GFIVLKNWLVRMERENALPRIVVRDMVSGAEHAIAFDEEAYSLGFGEMREYDTDVLRF
                     TYSSMTTPSQVFDYNMRDRTRVLRKMQEIPSGHDPKAYVTRRVLAPAHDGEAIPVTLL
                     YRKEAKLDGSEPLWLYGYGSYGISLPAGFNSNILSLVDRGFIYAMAHIRGGQE"
BASE COUNT         1082 a         1825 c         1861 g          984 t
ORIGIN      
        1 tggcttccaa ttgttccggc tctatcgcga gagttccgga aggaccgtca agcggccagc
       61 cgaagacgaa gaccccatgc tgcgcctcgc cctgtctggc ctcaatgaca cgcagatctc
      121 acgcctcatg aaggccgcgg aagtcaagga atgcgtcagt ggcgacgtgc tcacgcgaca
      181 gaagaagccc gtcaactcgc tttacttcct ttacgcaggc cgggtgaacg tggaagtcga
      241 cggcaagacc gtcgcagcac tggacaacgg cgccttcatc ggcgagatcg cctacctcac
      301 gggcaagcct gctacagcca ccgtggtggt cgacaagccg tcgcgcgttc tcaccttttc
      361 gcgcgccagg ctgaacaagg tgattgaaga ggatccgcag atcagcggca tcatctatga
      421 acttctgggc cgggacctgg cgcgcaagat gggccaggtg aattcgcagc gcaccgcatc
      481 cgacctgcag accgcgcgca tctgaaccac ctgccgtcaa gaacggggct gaccgcagcg
      541 caacctggac gcgccatctt gacaaggtac aacacaagat gtccccttgc tcaaccgaaa
      601 gcaaagggag tgcgggatgg actactttgc tccaagcaac gaatggtttg gccacctggc
      661 ctatgcgtgc ctcgccgcct cctacttcat gaccaacatc ttctggctgc gggtgctggc
      721 cgtggtcagc atgctgctcg aacttgcata cttcaactat gcgggcggcg atctcaccac
      781 cggcatgtcc tggaacgccg tcttcgttgc catcaacctg tatcagctgt tctggctgtt
      841 gcgcgaccgc ctgacgctga aactcccgga acatgacggg ccggtactcc gcagcgcact
      901 caccggcctc gacgacgccc agatcgcccg gcttctcaag gccgcgcact ggaagtcggc
      961 agccaagggc aagacgctta ccacgcagca caagccggtg gatgaactct acttcatctg
     1021 ttcgggcagg ttgagcgtgc aggtcgacgg ttcggtgatg gcccatcttg agaaggggtc
     1081 ctttgtcggc gagattgcct atctgacggg caacccggcg acggcgacgg tcgtggtcga
     1141 ggagccatgc cgcatcctgg ccttttcgcg gacgcagctg gcaaaggtgg tcgcgtccga
     1201 cacccacatc agcggcctca tctatcagat gcttggccgc gacctcgcaa tgaagatggg
     1261 tcaggccaat cggcgtcata cggccggacc gaacaacctg gcacaggtct aggacgatgc
     1321 ttttatcagt aggcggactc ttcgaatcct agttccgcgg tgcgtaaggg ccggacttgc
     1381 gcacgtacca gtcccagtag gtcaggagaa tgcgcgcctt gttgaaggac aggctgtcga
     1441 agacattctg cagctcctcg atgacgcggc cgggccggat gatcgcattc tcgccgcttc
     1501 ccagcttcag catggcctcc gacagctggc cgcgcggata acccagcgcc ttcaggatca
     1561 cggccatggg ctcgccctcc cggtcggcaa agatacgcag ggccgtgtcg cgactgatgc
     1621 cggcggcctc ggccaggacc tgggcgcctt cgtccaggcg gaactcggtg acgaggctca
     1681 gggcggcctc gatcgtatcg cgtggcggga agcgggtgtc ggcgggtgga atgtcgccgc
     1741 cgcccgtggc cagcgtgatg cgcaggatct tgttgagcgt ctcggaatcg gtaaggaagc
     1801 gcgagaggat gtagcggcgc agttccggcg gcacgaacca gaaaagttcg aaggcaaccg
     1861 gcggcgggag gtccggccgg gtcaccaggg gtgcgaggat ggcatgatgc tcgctcgcca
     1921 cttcgccgag cacgtagaag gcatcatgcg agaaggaagc gccgggattg cgcaccaggg
     1981 tgagcacgac cgatgggtct gcaagggcga tcaatctatc cgcgaggacc ggggagatca
     2041 cgcggcgcct ggcgatcatg cggcgcttgc cggcatccgc ttcattcgcg gccgtgatga
     2101 gatcctgatc ggtgatgtgc atgcaacgct cgagcagcgg cgccaccacc tcgggccgcg
     2161 gatcgcgaat cagctttgcc accagcagag ccggcggggt ttccatgatg gctacccgct
     2221 cgacgacggc aagcagctgc ttcaccggaa tgcgcggcag gatccggagc aaggtatcgg
     2281 cagcgagcgc gcgttcctgg ggaagaccgg atgccgcgcc catggacatc atctccagaa
     2341 gcgaaagggc cagttctccg ctctcctcgt cggcctccgc agtctcggtg ggcttcggct
     2401 cggccagcgc cttgaaggtc acggcaaatg gatcgggctc gggtttcggc ttcaatagca
     2461 ccgaaggcgg tggcgggggg gcaatcggta ctgcctcgat gaccggagcc aggacgggcg
     2521 cggaaggcgg gacggccggg gacacgggcg attcgggctg cgcctccgcc ggaacttcag
     2581 gctcgctttc ggccatttcg gccagttcgc gcaggaaggc attgcgttcg gccgccgtcg
     2641 gcttggcata gaccgagtcg gcgatcgtct tgatcatcgc ctcgaagacg cggtcggcaa
     2701 tgggcgacac aggcttcgtg gactcggcca cctgcaccgc gggcaggacc gtcggaatct
     2761 ggtgctcggg cttgggcgcc accggctcgg ggacgggcgg tggcagttct tcgatcacct
     2821 cttcgggtgc gacggcagct tccgcggcgg gctcgtgaac ggcttcgccg gccacgggtt
     2881 gcagcacctc ttcggcaatc gcctccagcg ccggctctgg ttcggccggg gattcgacca
     2941 gcgacgggaa gatcgcgtcg gaggactctt ccgccacgac ctcagctgcc tcgacctgca
     3001 ccggggcgtc ggcatacgcc gcttcaggtt gctctgattg cgccgcttcc gcaatgaccc
     3061 catcgtcttc gacacggcca aagggatccg gttcgtcgcg gagatcggtg gcgagggctt
     3121 ccgccggtgg ctccggctgg gcaaaaacct gggtgggttc aaaggcgggg aatgctggct
     3181 ggtcgggccg gaaatgggga atttcgggga actgcggcag ggaaaaggaa gccggttcca
     3241 ccggggattc cggcgtatca gcagcgtgct cgggaagcct ggcttccatt tccgggaccg
     3301 gtgcgagatc cggcatgtcc ggtacgacaa tgtcggcgtc ggccggggcg gcgatatcca
     3361 cctcgggcgg cgctgcagcg atttcatcct caaccggaac tgccggcgct tcggctggcg
     3421 gttcgagcaa gtcgggaatg gcgatttcac cagccctgag ggcggcaagt atgggtggtt
     3481 cggactcccg gcgcttgagc ttggcggtca gctggcgaaa gtggccggcg gagatgcggc
     3541 cgtgcggacg cgatcccacg gccgcgttcg ccgaatccgt cacttcccgc ggatcagcgc
     3601 ccctgggcgc ctccgcctca actggcaaaa ggctgtgcaa cagtattctc caccaatgcc
     3661 agctaaaacc gggaagcctc taaagatgct taaatccagc cgaaatgcct catttcacgg
     3721 cccttgcgct gggaacatgg taaatggccg cttaacggcg cacgaaagcg tgaggtcctg
     3781 tgccttcact ttgccgacct catcgcccat cttaggcgca gtccggagac ctgaagaatg
     3841 accaccaaga tcgacaagac ggatgccgac tggcgcaaga gcctgtcgcc cgaggcctat
     3901 cgcgtgctgc gcgagcacgg aaccgagcgg gccttcacca gcccgctgaa cgacgaaaag
     3961 cggaccggca tgttcacctg cgccggctgc ggaaaagagc tctttgcctc ggacgccaaa
     4021 ttcgagtcgg gcaccggctg gccaagcttc tatcaacctg cgtgcggtga cgccgtggga
     4081 accacagagg accggtcctg gttcatgcgg cggacggagg ttcactgcgc cgactgcggc
     4141 gggcacctcg gccatgtctt cccggacggg ccaaagccca caggcttgcg ttactccatc
     4201 aatggcgtat cgctcggctt ccggccagac aaggactgac atcatgacga aaaccattcc
     4261 catgccgccg cgggcggaga agcagccgcg cgtcgaaacc tggcatggcc atgagaaacg
     4321 cgacgactat cactggctga aggccgagaa ctggcagcag gtcatgcagg accccgcggt
     4381 gctgccgtcg aacatccgcg ctttcctcga gggcgagaac gcctaccacg aggccatcat
     4441 ggccgatacg gcggcgctgc aggagaagct cttcgccgag atgaagggac gcatcaagga
     4501 ggacgactcc tccgttcccg cgcccgatgg gccatggtcc tactattcgt ccttcgtcac
     4561 cggcgggcag catccgcgct acctccgcaa agcgcgcgat ctgaccggcc ccgagcagat
     4621 gatcctcgac ggcaatgcgt tggccaaggg ccgggagtac ttccgtttcg gcggcatcgc
     4681 catgtcgccc gaccagcggc tggccgcctg gtccttcgac ggcaacggct cggagttcta
     4741 cgaactcaag gtgcgcgacg tggcggcggg gtccgacctg ccggatctgc tgcaaaacac
     4801 tgcgggcggc gccacctggg cggcggacgg caagaccttc ttctacacgc tgcaggacga
     4861 gaaccatcgc ccgctgaaga ccttccggca caggctggga aaggcgcagg atgcggacgt
     4921 actggtctac gaggaacggg acaccgggat gttcaccggc gtgggcaaga ccaattcgga
     4981 cgcctacatc gtcatcgacg tgcatgacca cgacacctcc gagatccgca tcatcgatgc
     5041 gaaggcgccc gaggctgaac cgaggctcat cgcgccgcgt caggtgggtg tcgagtacga
     5101 cctcgagcac tggaatgatc gcttcgtcat caagacaaac atcggcggcg cggaagacta
     5161 caagatcgtc accgcaccgg tcgcgaaccc ggaccccgag aactggaccg acctcatccc
     5221 gcaccgcccg ggcatcttcc tcgtcggctt catcgtgctg aagaactggc tggtccgcat
     5281 ggagcgcgag aatgccctgc cgcgcatcgt ggtgcgcgac atggtcagcg gcgcggaaca
     5341 tgccatcgcc ttcgacgagg aggcctattc gctgggtttc ggcgagatgc gcgaatacga
     5401 cacggatgtg ctgcgcttca cctattcctc gatgaccacg ccctcgcagg tcttcgacta
     5461 caacatgcgc gaccgcacgc gggtgctgcg caagatgcag gaaatcccca gcgggcacga
     5521 tcccaaggcc tatgtcacgc ggcgcgttct ggccccggcc catgacggcg aggccattcc
     5581 cgtcaccctg ctctaccgca aggaggcgaa gctggacggc tcggaaccgc tgtggctcta
     5641 tggctacggc tcctatggca tttcgctccc cgccggattc aacagcaaca tcctgtcgct
     5701 ggtggatcgc ggcttcatct atgccatggc ccatatccgc ggcgggcagg ag
//
LOCUS       DBNGVR010000457         3664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4886711, whole genome shotgun sequence.
ACCESSION   DBNGVR010000457 DBNGVR010000000
VERSION     DBNGVR010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4886711"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..678
                     /gene="lptE"
                     /locus_tag="AC16A0_09725"
     CDS             172..678
                     /gene="lptE"
                     /locus_tag="AC16A0_09725"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HIR3105655.1"
                     /translation="MMRKRPAVLIVVVALLLAGCGYRPLYSSSAENQGVAAIMASVEI
                     PEAETRVGQIIRNDLISAMRPGNDGAGDRYLLNITPVMKTTEVIDKKQPSTTRESVRL
                     TVAYELIDQQTGQTIHSGKTFSQVSFDVVRQPFADTQAENNAMERAAHEVSGDIRMRI
                     AAFFATRA"
     gene            675..1715
                     /gene="holA"
                     /locus_tag="AC16A0_09730"
     CDS             675..1715
                     /gene="holA"
                     /locus_tag="AC16A0_09730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3105656.1"
                     /translation="MTAIRPQGLEGFLRKPDPAVGILLIYGDEPAAIRDLAARAVKQV
                     AGSLDDPFTVVRLDDGALAADPARLADEMQSISMFGGRRAIWVREAGEAFLRAAEPVL
                     DGRASGNLIVAEAGALAKSSALRNALEQSPRALILPVYEADDADISSMIEQTLAKAGL
                     AIDPDAKFLLMERVGTGRALMQRELEKLSLYAMGQDRIGLADVEASCSGGAEFETGDL
                     ADAMFAGEIEDSDRMFGVLVQSGIDAGRLLGAAHLHALKLQDFRLAIDAGSRMEQVLR
                     GARPPIFFKRMPAFQLQLGLWPLANLMQASTTLAAAVTQARLNASLAEAIANRAVLAV
                     ARNARALAADRR"
     gene            complement(1719..2618)
                     /locus_tag="AC16A0_09735"
     CDS             complement(1719..2618)
                     /locus_tag="AC16A0_09735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR3105657.1"
                     /translation="MSRRFLSIGECMVEMASTGGDTFRQGFAGDSFNTAWYARRALPA
                     DWDVDYYTTLGDDAVSGRMLRFMQDQSIGTGHIRRLAGRAPGLYMIELENGERSFTYW
                     RDTSAARSLADDAGALSAALAQADAIYVSGITLAILAPERRRTLLDGLGRAKAAGKMV
                     GFDSNIRMRLWSNTDDLRAAIKDAARVATISLPTIPDESDLFGEATAKEVSTRYEQWG
                     CQEVVVRSGGGPALVVQRGKTIAVAPTQVVAPVDTTGAGDSFNGTYLAARLTGESPEA
                     AARRAHAVAGKVIRHYGALVDPA"
     gene            complement(2773..3414)
                     /locus_tag="AC16A0_09740"
     CDS             complement(2773..3414)
                     /locus_tag="AC16A0_09740"
                     /inference="COORDINATES: protein motif:HMM:NF018473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HIR3105658.1"
                     /translation="MVELFSSQGCSSCPPADAFLAELRRLPHVLALTLHVDYWDYLGW
                     KDTLATPEFSQRQYDYAKARGDMDVYTPQLIVNGERHFVGSARDKVLAAVEAARKTPM
                     PIAVSLDHNDKEIVVEVGAGEGHGDATLWLMPIMPVIKVKILKGEIAGHEIEYHNVVR
                     KILPGGMWSGKASRFVLPTDGVMPPDCTACVALLQRGKVGPILGAAAWGNLSA"
BASE COUNT          649 a         1239 c         1167 g          609 t
ORIGIN      
        1 cgccgtgcag gtcaatggca agcgccgcga cgaggtcacc gtggccaagg atctcgacgc
       61 caaggccgtg gaggccatgg tgctgaagct cgagtcggtc gcccgtgcgc tggaaggacg
      121 ccccgtcaag aaagtgattg tggtgcccgg ccggatcgcc aatattgtcg gatgatgcgc
      181 aagaggcccg cagtcctgat cgtcgttgtc gcactgctcc tggcaggctg cggctatcgt
      241 ccgctctact cctcgtcagc cgagaatcaa ggcgttgcgg cgatcatggc gagtgtcgaa
      301 atcccggaag ccgagacgcg agtaggccag atcatccgca atgacctcat ttcggccatg
      361 cggccgggaa atgacggcgc gggcgaccgc tatctcctca acatcacgcc ggtgatgaag
      421 accaccgaag tgatcgacaa gaagcagccg tcgacgacgc gcgaatcggt ccggcttacg
      481 gtcgcctatg agctgatcga ccagcagacc gggcagacga tccacagcgg caagaccttc
      541 tcgcaggtct ccttcgacgt ggtgcgccag cccttcgccg atacccaggc cgagaacaat
      601 gcgatggaac gcgcggccca tgaggtgagc ggcgatatcc ggatgcggat tgccgccttc
      661 ttcgccaccc gcgcatgacc gcaatcaggc cccagggcct cgaggggttc ctgcgcaagc
      721 cggaccccgc ggttggaatc ctgctgatct acggcgacga gccggcggcg atccgcgatc
      781 tcgcggcccg cgccgtgaag caggtggcag gcagcctcga cgaccccttc accgtggtgc
      841 ggctggatga cggggcgctc gccgccgacc ccgcacggct ggcggacgag atgcagtcca
      901 tttccatgtt cggcggccgc cgggcgatct gggtgcgcga ggcgggcgaa gccttcctgc
      961 gcgcggcgga gcccgtgctg gatggccggg ccagcggcaa tctcatcgtt gccgaggccg
     1021 gcgcgctcgc caagtcttcg gcgctccgca acgccctgga gcagagcccc cgcgcactga
     1081 tcctgccggt ctacgaggcc gatgacgccg atatctccag catgatcgag cagacgctcg
     1141 ccaaagccgg tctcgccatc gatccggacg ccaagttcct tctcatggaa cgcgtgggca
     1201 ccggccgtgc cctcatgcag cgggagctgg aaaagctgtc gctctatgcc atggggcagg
     1261 accgcatcgg tcttgccgat gtcgaggcca gctgcagcgg cggagccgag ttcgagacgg
     1321 gagacctggc ggatgccatg tttgccggcg agatcgagga cagcgaccgc atgttcggtg
     1381 tcctggtcca atccggcatt gacgccggcc ggctgctggg tgctgcccat ctgcacgccc
     1441 tgaagctgca ggatttccgc ctggccatcg acgccgggag ccggatggaa caggtgctgc
     1501 gcggcgcgcg tccgccgatc ttcttcaagc gcatgcccgc cttccagctg cagctgggcc
     1561 tctggcccct cgccaatctc atgcaggcgt cgacgacact tgctgcggcc gtgacccagg
     1621 cccggctcaa tgcctcgctg gcggaagcca ttgccaaccg ggccgtgctg gcggtggcgc
     1681 gcaacgcccg ggccctggcg gccgaccggc gctgagcttc acgccgggtc gaccagcgca
     1741 ccgtagtggc ggatgacctt gcccgccacg gcatgggccc tgcgggccgc ggcttccggg
     1801 ctctcgcccg tcaatcgggc ggcaaggtag gtcccgttga aactatcccc tgcgccggtc
     1861 gtgtccacgg gtgccacgac ttgtgttggg gcgaccgcga ttgtcttgcc gcgctgcacg
     1921 acaagcgcgg gaccaccacc cgacctgaca acaacttctt gacagcccca ttgttcatat
     1981 cgagtcgata cttcttttgc cgtcgcttcg ccaaagagat cagattcgtc ggggatagtt
     2041 ggaagcgaaa tcgtagcgac tcgcgctgca tctttgattg ccgcgcgcag atcatctgta
     2101 ttggaccaga gacgcatcct gatattgctg tcaaaaccga ccatcttccc cgccgccttg
     2161 gctcgcccga ggccgtcgag cagggtccgc cgccgctccg gcgccagaat ggccagggtt
     2221 attccggaaa catagatcgc atctgcctgc gcgagggccg cagacagagc cccagcgtca
     2281 tctgccagtg accgggccgc cgaggtgtcg cgccaatagg tgaagctgcg ttcgccattc
     2341 tccagttcga tcatgtagag gcccggcgcc cgcccggcga ggcggcggat atggccggtg
     2401 ccgatgctct ggtcctgcat gaagcgcagc atgcggccgc tgaccgcatc gtccccgagg
     2461 gtggtgtagt agtcgacgtc ccagtcggcc ggcagggcac ggcgggcata ccacgccgta
     2521 ttgaagctgt ccccggcgaa gccctggcgg aacgtgtcac caccggtgga ggccatttcg
     2581 accatgcact cgccgatcga caggaaacgc ctgctcatgc gcgccttgcg ctcccccaag
     2641 ggcggctccg ccgcctgcac gccatgactg atatcacatc actgcgcccc gacaatgcct
     2701 ccgccgaacg gcgggtcaaa ggcgttccaa cgccgccatt gccgcgcttc gcatgaagcc
     2761 ggcagcctgt gttcaggcgc tcagattccc ccaggcggca gccccgagaa tgggcccgac
     2821 cttgcccctc tgcagcaacg ccacacaggc ggtgcaatcc ggcggcatca ccccatcggt
     2881 cggaagcacg aatcgcgacg ccttgccgga ccacatgccg ccgggaagga tcttgcgcac
     2941 cacgttgtga tactcgatct cgtgtccggc gatttcgccc ttcaggatct tcaccttgat
     3001 caccggcatg atcggcatca gccacaacgt cgcgtcgcca tggccctcgc cggcacccac
     3061 ctcgacgaca atctccttgt cgttgtggtc cagggacacg gcaatgggca tcggagtctt
     3121 ccgtgcagct tcgacggcgg ccagcacctt gtcgcgcgca ctgcccacga aatgcctctc
     3181 gccgttcacg ataagctgcg gcgtgtagac atccatgtcg ccccgcgcct tggcatagtc
     3241 atactggcgc tgcgagaact ccggcgtggc cagcgtatcc ttccagccaa gatagtccca
     3301 gtaatccaca tgcagggtca atgccagcac atgcggaagg cggcgcaatt cggcgagaaa
     3361 ggcatcggcc ggagggcagg atgagcagcc ctggctgctg aacagctcga ccacgaccgg
     3421 cgaatccgcc gccgacgcgg gccgggacag gaggctaaag ccaagccccg caccagcggc
     3481 cagaaggcca cggcgggata tggttcgggc ggaagacatg ctgcctatat ggcgcaaacg
     3541 gcccgtgagc gccactcacg agccgttgag ccggtatcac gaacacgtat tggcggaatc
     3601 aggccgccct ggccaggttg cgcagcacat agggcatgat gccgccgttc ctgtagtagt
     3661 cgag
//
LOCUS       DBNGVR010000458         2267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4886718, whole genome shotgun sequence.
ACCESSION   DBNGVR010000458 DBNGVR010000000
VERSION     DBNGVR010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4886718"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="AC16A0_09745"
     CDS             <1..402
                     /locus_tag="AC16A0_09745"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR3105659.1"
                     /translation="AAARGEVPVGAAIVDRSGAILASAGNRTLELKDPTAHAEMLVIR
                     AAARILGSERLVGCDLYVTLEPCAMCAAAVSLARLRRLYFAAEDEKMGAVEHGPRFFG
                     QPTCHHRPDVYGGIGEERSRILLREFFSSKR"
     gene            complement(386..1234)
                     /locus_tag="AC16A0_09750"
     CDS             complement(386..1234)
                     /locus_tag="AC16A0_09750"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3105660.1"
                     /translation="MSGLRIGLALGGGSARGLAHIPMLEVFDELGLQPSVIAGCSIGS
                     LIGSAYASGMSARDIRDHAELLLNNRIDAFRYVFGAKRARLRDLLALRGLASFHVQGE
                     KLVELALPEGVPALIEHTRIPFKVIATDYDVMEERVFVTGPLVRAVGASIAIPGVILG
                     PTIDGHVHVDGGVTNPVPFDHVRDGTDLVVAVDVTGRPRPPVRLHHSNMELAIGSLLI
                     MFHQVAELRRRLTPPDIYVAPPLLAFSGGDFFKLKELFAAAQPAKDQLKRELDSIITK
                     RLTASN"
     gene            complement(1231..>2267)
                     /locus_tag="AC16A0_09755"
     CDS             complement(1231..>2267)
                     /locus_tag="AC16A0_09755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3105661.1"
                     /translation="GTTAADSPAAALCDYLSNSIDGFEGPLEISRFKGGQSNPTYRLG
                     TPRRHYVLRRKPFGKLLPSAHAIEREYRITDALSLAGFPVARPLHLCTDDSVIGAAFY
                     VMEHVEGRVFWEPWAPGISPPARSAIFKSMNHTLARLHGFDFAAAGLSDFGKPQGYVA
                     RQIRRWSEQYRASETERIADMDRLIAWLPDAAPPDSGAAVVHGDFRLDNCIIHPAEPR
                     VSAVLDWELATIGDPVADFTYLLMPWFMPVSETGAGVGSLVGHTEDPGIPSFESYIED
                     YCTQTGRAALPSLNTYMAYNFFRLAAILQGILGRVRDGTAASAEAATMARQVRPLAET
                     AWHFAKKAGA"
BASE COUNT          424 a          692 c          766 g          385 t
ORIGIN      
        1 gccgccgcgc gcggggaagt gccggtcggt gcggcaatcg tcgatcgcag cggtgccatt
       61 ctcgccagcg ccggcaatcg cacgctcgag ctgaaggatc cgaccgcgca cgcggaaatg
      121 ctagtcatcc gggccgcggc gaggatcctc ggcagcgaaa ggcttgtcgg ttgcgatctc
      181 tacgtcacat tggaaccatg tgccatgtgt gcggctgccg tgtccctggc gcgccttcgc
      241 cggctctact ttgccgcgga agacgaaaag atgggggccg tcgagcatgg tccgcgcttc
      301 ttcggccagc cgacctgcca tcaccgtccg gatgtgtatg gcggaatcgg agaagagcga
      361 tcgcgcattc tgctccgcga gttcttcagt tcgaagcggt aaggcgcttg gtgatgatgc
      421 tgtcgagttc gcgcttgagc tggtccttgg ccggctgggc agctgcgaag agttccttca
      481 gcttgaagaa atcgcctccc gagaaagcga gcaaaggcgg cgccacgtag atgtcgggcg
      541 gcgtcagcct gcggcgcagc tcggccacct gatggaacat gatgagtagt gacccgatgg
      601 caagctccat gttggaatga tggaggcgga cgggtggccg tggccggccg gtcacatcga
      661 cggccaccac gagatcggtg ccgtcgcgca catgatcgaa gggcaccgga ttggtgacac
      721 cgccatcgac atggacatgg ccatcgatcg tcggcccgag aatcacgccg gggattgcga
      781 tcgaggcgcc caccgcgcgg accaatggtc cggtgacgaa gactcgctcc tccatcacgt
      841 cgtagtccgt cgcgatgacc ttgaagggga ttcgggtgtg ctcgatcaga gccggaacgc
      901 cctcgggcag cgcgagctcg accagtttct ccccctgtac atggaaggag gcaaggccgc
      961 gcagggccag cagatcgcgc agtctggcgc gcttggcgcc gaagacatag cggaatgcgt
     1021 cgatgcggtt gttcagcagg agttcggcat gatcacgtat gtcgcgggcg ctcatgccgc
     1081 tggcataggc cgatccgatc agcgaaccga tcgagcagcc ggcaatgacg gatggctgga
     1141 ggccgagctc gtcgaagacc tcaagcatgg ggatatgcgc aagcccgcgc gcactgccgc
     1201 cgcccagtgc caggccgatc ctgagaccgc tcatgcgcca gccttttttg cgaaatgcca
     1261 ggccgtctcg gccagcggcc gcacctgccg cgccatagtg gcggcctcgg cactggccgc
     1321 cgtgccgtct ctgacccggc ccagaatgcc ctgaaggatc gcggcgagcc ggaagaagtt
     1381 ataggccata taggtattca agcttggcag cgcggcgcgt cccgtctgcg tgcaataatc
     1441 ctcgatgtag ctctcgaaac tgggaattcc gggatcttcc gtgtgcccca caagcgagcc
     1501 cacgccggct ccggtttccg agacgggcat gaaccagggc atgagaaggt atgtgaaatc
     1561 tgcgacggga tcgccgatcg ttgcaagctc ccagtccagg actgcggaaa cgcgaggctc
     1621 cgcaggatga atgatgcagt tgtcgagacg gaaatcgcca tggaccaccg cggcccccga
     1681 gtcgggcggt gccgcatccg gcagccaggc gatgaggcgg tccatgtcgg cgatgcgctc
     1741 tgtctcggac gctctgtact gctcgctcca gcgacggatc tggcgggcaa catagccctg
     1801 cggcttgccg aagtcggaaa gtccggcagc cgcaaagtcg aacccatgaa gccttgcaag
     1861 cgtgtggttc atggatttga agatcgcgct gcgcgccggc ggagagatgc ctggcgccca
     1921 gggctcccag aagacgcggc cttcgacatg ctccatcaca tagaaggccg ctccgatgac
     1981 agagtcatcc gtacaaagat gaagcggccg cgccaccgga aacccggcga gggacagtgc
     2041 gtccgtgatc cggtactcgc gctcgatcgc atgggcggaa ggcagcagct tgccgaaggg
     2101 cttgcgacgc agcacgtagt gcctccgcgg ggtgccgagg cgataggtcg ggttcgactg
     2161 accgcccttg aagcgcgaga tctccagcgg tccttcgaag ccatcgatgc tgttggagag
     2221 gtagtcgcac agcgccgcgg cgggactgtc ggctgcagtc gtgccgg
//
LOCUS       DBNGVR010000459         1721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3511351, whole genome shotgun sequence.
ACCESSION   DBNGVR010000459 DBNGVR010000000
VERSION     DBNGVR010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3511351"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /gene="hrcA"
                     /locus_tag="AC16A0_09760"
     CDS             complement(<1..427)
                     /gene="hrcA"
                     /locus_tag="AC16A0_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168608.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3105662.1"
                     /translation="MSIIPPKPFLDEKLPGIAGLDQRSRDIFRRLVDTYLETGEPVGS
                     RTISRILPQSLSPASVRNVMMDLEEAGLIFSPHTSAGRVPTDLGLRFFVDALKEVGSM
                     SAEERARIDAQIAASNRPRRIEDVLGEATTLLSGLSHCAG"
     gene            505..1218
                     /gene="rph"
                     /locus_tag="AC16A0_09765"
     CDS             505..1218
                     /gene="rph"
                     /locus_tag="AC16A0_09765"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR3105663.1"
                     /translation="MRPSGRKPDQLRAVSFERGFTKHAEGSCLVRFGDTHVLCTASLE
                     ERVPPWLKGAGKGWVTAEYGMLPRATGERTRREASAGKQSGRTQEIQRLVGRSLRSVV
                     DLVALGERQITLDCDVLQADGGTRTAAITGSWVALHDCLSWMQARSMFKSFPMKDHVA
                     AISCGIHNDLAVLDLDYAEDSTAGTDANFVMTGTGGIVEIQGTAEGGPFSEANLLELL
                     MLAKKGIGELVALQRQAIA"
     gene            1218..>1721
                     /locus_tag="AC16A0_09770"
     CDS             1218..>1721
                     /locus_tag="AC16A0_09770"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HIR3105664.1"
                     /translation="MRKLSGERIVIATHNKGKLAEFADLLKPYGVTAVSAGELGLAEP
                     EETESSFAGNARIKALAAMKASGLIAMSDDSGLCVDALEGAPGVYTADWAGPARDWTA
                     AMGLVQAKLAAIGAVTPDRRRASFVCTLCVIWPDGEEHLYEGRAPGHLVWPPRGTLGH
                     GYDPMFVP"
BASE COUNT          295 a          545 c          590 g          291 t
ORIGIN      
        1 cgccggcgca gtgtgaaaga ccggacagca gcgtcgtggc ctcgcccagc acgtcttcga
       61 tgcgccgtgg ccggttggag gcggcgatct gggcgtcgat gcgggcgcgc tcctcggcgc
      121 tcatcgatcc cacctccttg agggcatcga caaagaagcg caggccaagg tcggtaggca
      181 cgcggccggc actggtgtgc ggcgagaaaa tcagccccgc ttcttccaga tccatcatga
      241 cgttacgcac cgaggccggc gacaggcttt gcggcaggat ccgggagatg gtgcgcgatc
      301 ccaccggctc gccggtctcc agataggtgt cgaccagccg ccggaagatg tcgcgcgagc
      361 gctggtcgag cccggcgata cccggaagct tttcgtcaag gaaaggcttg ggcggaatga
      421 tgctcatggc gccaatgtag aaatcaagtt ggctgaggtc aattgtgggt ctagaagacc
      481 ccccttcacg aaaggaacca gatcatgcgt ccatcgggac ggaaaccgga ccagttgcgc
      541 gccgtcagct ttgagcgcgg cttcaccaaa catgccgagg gctcctgcct cgtccgcttc
      601 ggcgacaccc atgttctatg cacggcctcg ctggaggagc gcgtgccgcc ctggctcaag
      661 ggcgccggca agggctgggt caccgccgag tacggcatgc tgccccgggc cacgggcgag
      721 cgcacacggc gcgaggcctc ggccggcaag cagtcgggcc ggacgcagga gatccagcgc
      781 ctggtcggcc ggtcgctccg ctcggtcgtg gatctcgtgg cgctgggcga acgccagatc
      841 acgctcgact gcgacgtgct gcaggccgat ggcggcaccc gcaccgccgc catcaccggt
      901 tcgtgggtgg cgctccatga ctgtctctcc tggatgcagg cacgctccat gttcaagagc
      961 ttcccgatga aggaccatgt ggcggcgatc tcctgcggca tccacaatga cctggcggtt
     1021 ctcgatctcg actatgccga ggactcgacc gccggaaccg atgccaattt cgtcatgacc
     1081 ggaaccggcg gcatcgtcga gatccagggc acggcggaag gcggtccctt ctccgaggcc
     1141 aatctgctcg aattgctgat gctggccaag aagggcatcg gcgaactggt ggccctgcag
     1201 cgccaggcga ttgcgtgatg cgcaagctga gcggtgagcg aatcgtgatc gccacccaca
     1261 acaagggaaa gcttgccgag ttcgcggacc tgctgaagcc ctatggcgtg acggcggtgt
     1321 cggcgggtga gttgggtctt gctgaacctg aagaaaccga gtcgagtttc gccggcaatg
     1381 cgcgcatcaa ggcgcttgcc gccatgaagg cctcgggcct catcgccatg tcggatgatt
     1441 ccggcctctg cgtcgatgcg ctcgaaggtg cgcccggcgt ctatacggcg gactgggcag
     1501 gaccggcgcg cgactggact gcggccatgg gtctggtcca ggcgaaactc gccgcgatcg
     1561 gtgccgtgac gccggaccgg cgccgcgcat ctttcgtgtg cacgctctgc gtcatctggc
     1621 cggacggcga agagcatctc tatgaggggc gcgctccggg gcatctggtg tggccgccgc
     1681 gcggaaccct ggggcatggc tacgatccga tgttcgtgcc c
//
LOCUS       DBNGVR010000460         1743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4887314, whole genome shotgun sequence.
ACCESSION   DBNGVR010000460 DBNGVR010000000
VERSION     DBNGVR010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4887314"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="AC16A0_09775"
     CDS             complement(<1..787)
                     /locus_tag="AC16A0_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3105665.1"
                     /translation="MNLEELRTFLEVVETGSLVMAAKRLNVTPSTVSARISGLEEEIG
                     QKLLHRNKSGAELTSPGFKFKRYAEVMLQLWGQARYEVSLPRGFEGVCNVGLDFDLWP
                     GVGQRFLEHLRRHAPGIAVALWPGEQRMIDRWLEIGLVDIAFCHAPPAGERFTSRVLF
                     DDALVMVSTRKAQAPDLSAGYVYVDHGDAFRRQHAAAFPGDVTSALTIASSDWALGHL
                     LRNGGSGYLPRRHVTDAIGDGQLHLVEGAPVLSRRVYLVETAQT"
     gene            890..>1743
                     /locus_tag="AC16A0_09780"
     CDS             890..>1743
                     /locus_tag="AC16A0_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3105666.1"
                     /translation="MDFIHFLSSHMLDPQIGGKRLYKSVVAQAVHAERLGYRGVGIPE
                     HHLLNILLIPSPLQMAVKVAAHTENLLLMTSVCQLPLRDMRIFAGEVVQAQALCDGRL
                     MLGVGKGAFAYETERIGVPFGDTKPRFEEDLKILEALLTRADVSWDSPRYSFAPLTIM
                     PRPEDPIPLMVAVMNPVGIEAAARAGYHVQTTPLGGTHQQLIDQVTAFNRGKAAAGRP
                     LDTRLSLQRGVFLVSSDAERRKIAERAFEYYKSFDNVFGGPGIVDQGIIRALPRSQTL
                     EELTGNLLL"
BASE COUNT          313 a          565 c          577 g          288 t
ORIGIN      
        1 ccgtctgcgc cgtctccacg agatagacgc ggcgggacag gaccggggcc ccctcgacaa
       61 gatgaagttg gccatcaccg atggcgtccg tgacatggcg ccgtggcagg tagcccgagc
      121 caccattgcg caggaggtgg cccagggccc agtcggagga tgcgatggtg agcgccgatg
      181 tcacatcgcc cggaaaggcg gcggcatgct ggcggcggaa ggcatcgccg tgatcgacat
      241 agacatagcc ggccgagagg tccggcgcct gtgccttccg ggtcgagacc atgaccaggg
      301 cgtcgtcgaa gaggacccgg ctggtgaagc gctcgcccgc cggcggggca tgacagaagg
      361 caatgtcgac caggccgatc tccagccagc ggtcgatcat gcgctgctcg ccgggccaca
      421 aggccacggc gatgcccggc gcatgacggc gcagatgttc gaggaagcgc tggcccacgc
      481 cgggccagag gtcgaagtcg agcccgacgt tgcagacgcc ctcgaagccc ctgggcaacg
      541 acacctcgta gcgcgcttgg ccccagagct gcagcatcac ctcggcatag cgcttgaact
      601 tgaagcccgg cgaggtgagc tcggcgcccg acttgttccg gtgcagcagc ttctggccga
      661 tttcctcttc cagcccgctg atgcgcgcgc tgaccgtgga gggggtcacg ttcagacgct
      721 tcgcggccat gaccaggctt ccggtctcga ccacttcgag gaaggtgcgg agttcttcga
      781 ggttcatggg ataagttcgg tgatttcgaa cataatgcca aaaatttttc gattttcaat
      841 cttttgggag tctggcactg tttccgcgcg acacagacgg ggaacggaca tggacttcat
      901 ccactttctg tccagccaca tgctggaccc gcagatcggc ggcaagcgcc tttacaagag
      961 cgtggtggcc caggcggtcc atgccgagcg cctcggctat cgcggcgtcg gaatccccga
     1021 acaccatctt ctcaacattc tgctgatccc gtcgccgctg cagatggcgg tgaaggtggc
     1081 ggcgcatacc gagaacctgc tgctcatgac gtctgtatgt cagctgccgc tgcgcgacat
     1141 gcggatcttc gccggcgagg tggtgcaggc ccaggcgctg tgcgacggcc ggctgatgct
     1201 gggcgtgggc aagggcgcct tcgcctacga gaccgagcgg atcggcgtgc ccttcggcga
     1261 caccaagccg cgcttcgagg aggacctgaa gattctcgaa gccctgctga cgcgtgccga
     1321 tgtctcatgg gacagtccgc gctacagctt cgcgccgctc accatcatgc cgcgtcccga
     1381 agatccgatc ccgctcatgg tggcggtgat gaaccccgtg ggcatcgagg cggcggccag
     1441 ggccggctac cacgtgcaga ccacgccgct gggcggaacc caccagcagc tcatcgacca
     1501 ggtgacggcc ttcaatcgcg gcaaggcggc ggccggacgg ccgctcgata cgcggctcag
     1561 cctgcagcgc ggcgtcttcc tcgtatcgag cgacgccgaa cgccgcaaga tcgccgaacg
     1621 cgcattcgag tattacaaga gcttcgacaa tgtcttcggc gggcccggca tcgtcgacca
     1681 ggggatcatc cgggcgctgc cgcgcagcca gacgctcgag gaactcacgg gcaacctgct
     1741 gct
//
LOCUS       DBNGVR010000461         4371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5279089, whole genome shotgun sequence.
ACCESSION   DBNGVR010000461 DBNGVR010000000
VERSION     DBNGVR010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5279089"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="AC16A0_09785"
     CDS             complement(<1..367)
                     /locus_tag="AC16A0_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3105667.1"
                     /translation="MSFRAIQISKTEQGQKADFVTLEESYLTEGDVLVEVSHSSVNYK
                     DGLAVTGKGPIIRKFPLIPGIDFAGTVRESSHPKWKAGDQVVLNGWGVGEQYNGGFAE
                     LARVSGDWLVPVPKGWTPAD"
     gene            complement(379..1704)
                     /locus_tag="AC16A0_09790"
     CDS             complement(379..1704)
                     /locus_tag="AC16A0_09790"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3105668.1"
                     /translation="MALTAELILRNGTVVNHDGVGQRDIAVSGGRIVGIGQFAAAQAG
                     EVIDCTGLHVLPGVVDSQVHLREPGAEHKEDLETGGRAAVMGGVTGVFEMPNTNPLTV
                     TAEALADKVRRATSRMQCDFAFYMGGTRDNAAHLGDFEQLPGCAGIKVFMGSSTGSLL
                     VEDDAGVADILASIRRRAAFHSEDEFRLNDRKSERRSGDAASHPVWRDAEAARLCTER
                     LLRLARAAGKRIHVLHISTADEFPLLAANRDIATVEVTPQHLSFALEDYARLGTLIQM
                     NPPVREAHHRLAIWKALQSGLVDVIGSDHAPHTLEEKAKAYPLSPSGMPGVQTLVPVM
                     LDFVNKGRLSIERFVDLTSHGPQRIFGIAGKGRIAEGYDADFTVVDLKARREIDNGWI
                     ESRCKWTPYHGMTVTGWPVGTIIRGTRVMWDGAITGAAQGQPIRFVEAL"
     gene            1816..2595
                     /gene="ygfZ"
                     /locus_tag="AC16A0_09795"
     CDS             1816..2595
                     /gene="ygfZ"
                     /locus_tag="AC16A0_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3105669.1"
                     /translation="MRLAGADSRHFLQNLLTADIDHLAPGAVTYAALLSPQGKILSDL
                     FVVAEADSFLIDCAASQKADLVKKLTLYKLRAAVTIADLPDKTIRVSPAEIPDGWRDP
                     RLSSMGWRGLSDRTSAPSPDYDAARIAAGLADSDADIGTNQLFPHEANLDQLGGVSFD
                     KGCYIGQEVVSRMEHRGTARSRILPLTLSGPAPVSSTEIRAGDKLVGTMLSSAGTRAL
                     GLIRLDRLAEAATPLLTDAVTVRVQKPAWVGYEVPGAEASA"
     gene            2592..3182
                     /locus_tag="AC16A0_09800"
     CDS             2592..3182
                     /locus_tag="AC16A0_09800"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR3105670.1"
                     /translation="MTAVRHEDGICRCGWCGTDPLYVAYHDTEWGVPEYDSQALFEKL
                     LLDGFQAGLSWITILRKRDNFRAAFDGFDPAKMARYTAKKQDRLMQDSGIVRNRLKIE
                     ASVSNAQAYLAIPDFSAYLWDFLDGQVITNHRRSMKDVPAKTPLAEKISKDLQKKGFR
                     FVGPTIVYAFMQACGLVNDHLVTCHRHQAVADAARR"
     gene            3166..3768
                     /locus_tag="AC16A0_09805"
     CDS             3166..3768
                     /locus_tag="AC16A0_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR3105671.1"
                     /translation="MPRAADEAPRAWQRMLSGRRLDLLDPSPLDIEIEDIAHGLARVA
                     RWNGQTSGDHAFSVAQHCVLVEQIAIHTEPHLDRAARLAALLHDAPEYVVGDMISPFK
                     AALGLDYKAFEKRLLAAIHIRFGLPPATPEKLEAAIKKADRVAAYLEATQLAGFSIPE
                     AEKFFGRPRAGSQKFHRLKPLPPTEAAALYLKAFRKLSDP"
     gene            3765..4268
                     /locus_tag="AC16A0_09810"
     CDS             3765..4268
                     /locus_tag="AC16A0_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phosphatase family protein"
                     /protein_id="HIR3105672.1"
                     /translation="MIIVCPLNKAEALVQQHGASHVISLLGPDTPHRRFGSVTPDRHL
                     RLTFHDIVQSSDGLQPASATDAESLVGFIANWPREKPLLIHCWAGISRSTAAAYTALC
                     MLRPAAAEEELAWELRESAPSATPNRLIVSLVDQRLGRNGRMQRAIEQIGRGADAFEG
                     TPFTLKI"
BASE COUNT          744 a         1520 c         1357 g          750 t
ORIGIN      
        1 agtcggcggg cgtccagccc ttgggtaccg gcaccagcca gtcgcccgaa acgcgcgcca
       61 gttcggcgaa gccgccattg tactgttcgc ccacgcccca gccgttgaga accacctgat
      121 cgcccgcctt ccatttcgga tggctggact cgcgcaccgt gccggcaaag tcgatgccgg
      181 ggatcagcgg aaacttgcgg atgatcggac ccttgccggt aacggcaagc ccgtccttgt
      241 agttgaccga tgagtggctc acctccacca gcacgtcacc ttcggtcaga tagctctcct
      301 ccagcgtcac gaaatcggcc ttctggccct gctcggtctt gctgatctgg atggcgcgga
      361 aactcatggt ctgtcctttc aaagggcttc gacgaagcgg atgggctgtc cctgagcggc
      421 accggtgatg gcgccgtccc acatgacgcg agttccacgg atgatggtgc ccacgggcca
      481 gccggtcacc gtcatgccgt gatagggcgt ccatttgcaa cggctttcga tccagccatt
      541 gtcgatctcg cggcgggcct tcaggtccac caccgtgaag tctgcgtcat agccttcggc
      601 gatgcggccc ttgccggcga tgccgaagat gcgctgcggt ccatggctgg tgagatccac
      661 gaagcgttcg atgctcaggc ggcccttgtt gacgaagtcg agcatcaccg gcaccagagt
      721 ctgcacgccg ggcatgcctg agggcgagag cgggtacgcc ttggccttct cctccagcgt
      781 atgcggcgca tggtccgaac cgatgacgtc gacgaggccg gactgcagcg ccttccagat
      841 cgccaggcgg tgatgtgcct cgcgcaccgg cgggttcatc tggatcagcg tgccgagccg
      901 cgcgtagtcc tcgagcgcga aagacaggtg ctgcggcgtc acttccaccg tggcgatgtc
      961 gcggttggcg gccagcagcg ggaactcgtc tgccgtcgag atatgcagca catggatgcg
     1021 cttgccggcc gcccgcgcca ggcgcagcaa acgttcggtg cagagccggg ctgcctcggc
     1081 gtcacgccac accgggtggc tcgccgcatc gccgctgcgt cgttcgctct tgcggtcatt
     1141 gaggcggaac tcgtcctcgc tgtggaaggc ggcacgccgc cggatcgacg ccaggatgtc
     1201 ggccacgccg gcatcgtcct ccaccagcag gctcccggtg gaggacccca tgaagacctt
     1261 gatcccggcg cagcccggca gctgctcgaa gtcgccgaga tgcgccgcgt tgtcccgcgt
     1321 accgcccatg tagaaggcga agtcgcactg catgcggctc gtcgcgcgcc gcaccttgtc
     1381 ggccagggct tcggcggtca ccgtcagggg attggtattg ggcatctcga agacgccggt
     1441 gacgccgccc atgacggcgg cgcggccgcc ggtctccaga tcctccttgt gctcggcacc
     1501 cggctcgcgc agatgcacct ggctgtcgac cacgccgggc aggacatgca ggccggtaca
     1561 gtcgatcacc tcgcccgcct gcgcggctgc gaactgcccg atgccgacga tccgcccgcc
     1621 cgagaccgcg atatcgcgct ggcccacccc gtcgtggttc accaccgtgc cattcctgag
     1681 gatcagctct gctgtcagtg ccatcgggct ttccttgaaa ctcctgtccc accttcgtaa
     1741 atgtggacgg ggatcgaagc aacacagatg cagaatcaaa tgtttacggc aggaacgcga
     1801 ccggaccggg ccgtcttgag gctggccggc gcggattcgc ggcatttcct gcagaatctg
     1861 ctgactgccg acattgacca cctggcaccg ggtgccgtca cctacgccgc cctgctctcc
     1921 ccccagggca agatcctctc cgatctcttc gtggtggccg aggccgatag cttcctgatc
     1981 gactgcgcag cctcgcagaa agccgatctc gtgaaaaagc tgacgcttta caagctccgg
     2041 gccgcggtca ccatagctga cctcccggac aagacgatcc gtgtgtcgcc ggcagagatt
     2101 cctgacggct ggcgggatcc ccgcctgtcc agcatgggct ggcgcggcct ttccgaccgg
     2161 acatcggcac cgtcgcccga ctatgacgcc gcccgcatcg cggcgggtct cgccgacagc
     2221 gatgccgaca ttggaacgaa ccagctcttc ccgcacgagg ccaatctcga ccagctgggc
     2281 ggcgtgagct tcgacaaggg ctgctacatc ggccaggagg ttgtctcgcg catggaacac
     2341 cgcggcaccg cccgcagccg gatcctgccg ctgacgcttt cggggccagc accggtgagc
     2401 agcacggaaa tccgcgccgg cgacaagctg gtcggcacca tgctgtcgtc cgcaggcacg
     2461 cgcgcgctcg gcctcatccg cctcgaccgc ctggcagagg ctgcaacccc cctgctgaca
     2521 gatgctgtca cggtccgggt gcagaaaccc gcctgggtag gttatgaggt gcccggggcg
     2581 gaggcgagtg catgacggcc gtaagacacg aggatggcat ctgccgctgc ggctggtgcg
     2641 gcacggatcc gctctacgtc gcctatcacg acacagaatg gggcgtgccg gaatacgact
     2701 cgcaggcgct gttcgagaag ctgctgctcg acggcttcca ggcgggactc tcgtggatca
     2761 ccatcctgcg aaagcgcgac aatttccgcg ccgccttcga tggcttcgat cccgcgaaga
     2821 tggcgcgcta cacggcgaag aagcaggacc gtctgatgca ggacagcggc atcgtgcgca
     2881 accggctgaa gatcgaggcc agtgtcagca atgcccaggc ctatctcgcg atcccggatt
     2941 tctccgccta tctctgggat tttctcgatg gccaggtgat caccaatcac cgccgcagca
     3001 tgaaggatgt gccagccaag acgccgctgg cggagaagat ctcgaaggac ctgcagaaga
     3061 agggcttccg cttcgtcggc cccaccatcg tctatgcctt catgcaggcc tgcggactgg
     3121 tcaacgacca tctcgtcacc tgccatcgtc atcaggcggt tgccgatgcc gcgcgccgct
     3181 gacgaggcgc cgcgcgcctg gcagcgcatg ctgtcgggcc ggcggcttga cctgctcgac
     3241 ccctcgccgc ttgacatcga gatcgaagac atcgcgcacg gacttgcccg cgtggcgcgc
     3301 tggaacggac agacatcggg cgaccacgcc ttttcggtcg cccagcattg tgtgctggtc
     3361 gagcagatcg ccatccacac ggaaccgcat ctcgaccgcg ccgcgcggct ggcggcgctg
     3421 ctgcacgatg cgccggaata cgtcgtgggc gacatgatct cgcctttcaa ggccgccctc
     3481 ggtcttgact acaaggcctt cgagaagcgc ctgcttgcgg ccatccacat ccgcttcggc
     3541 cttccgcccg cgacgccgga gaagcttgag gcggcaatca agaaagccga ccgcgtcgcc
     3601 gcctatctgg aggctacgca gctcgccggc ttcagcattc ccgaggccga aaaattcttc
     3661 ggccgcccga gggcgggctc gcagaaattc caccgcctga aaccgctgcc gcccaccgaa
     3721 gccgcagcgc tctatctcaa ggccttccgg aagctgagcg atccatgatc atcgtctgcc
     3781 cgctgaacaa ggccgaggcg ctggtgcagc agcatggcgc atcgcatgtc atcagcctgc
     3841 tcggccccga cacgccgcac cgccgcttcg gcagcgtcac gcccgaccgg cacctgcgtc
     3901 tcacctttca tgacatcgtg cagtcaagcg acggcctgca accggccagt gctacggatg
     3961 ccgaaagcct cgtcggcttc attgcgaact ggccgcgcga gaagcccctg ctcatccact
     4021 gctgggccgg catcagccgg tcgacggccg cggcctatac cgccctgtgc atgctgcgtc
     4081 ctgccgccgc tgaagaagaa ctcgcctggg aactgcgcga gtccgcgcca tcggccacgc
     4141 ccaatcgcct gatcgtgtcg ctcgtggacc agcgcctcgg ccgcaatggc cgcatgcagc
     4201 gcgccatcga acagatcggc cgcggcgccg atgccttcga gggcacgccc ttcacattga
     4261 agatctgaca gtccgccacg gccctcatcc ggcgctgcgc gccaccttct cccgcaagcg
     4321 ggagaagggt cattatcttg tccttctccc accgcaagcg ggagaaggat g
//
LOCUS       DBNGVR010000462         3513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4690153, whole genome shotgun sequence.
ACCESSION   DBNGVR010000462 DBNGVR010000000
VERSION     DBNGVR010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4690153"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..1271
                     /locus_tag="AC16A0_09815"
     CDS             480..1271
                     /locus_tag="AC16A0_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3105673.1"
                     /translation="MKRLFPWLGQLNRRTTEASADSDAASACRFNLASYNIHKCVGTD
                     GRFDPQRIKQVILALDADIVVLQEADARFGEREGLLDLSYLHAAGGYQSAVALSEGSR
                     SHGWHGNVVLYRSGIVHDVHQLTLPGLEPRGAVLVDFGIEQANFRLLAVHFGLLRQSR
                     MRQVQAIIDAADPHPDRHVIIVGDMNEWRLKNRSALDLFKHHFDEVSQNLPSYPSRLP
                     LLPLDRLFISRGLRSEGMSVIDTPLTRLASDHLPLTAKLSFQDRF"
     gene            1446..3023
                     /locus_tag="AC16A0_09820"
     CDS             1446..3023
                     /locus_tag="AC16A0_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HIR3105674.1"
                     /translation="MSATLAGLLADKDAESPAIGAPGRPWLTYGGLRSLSLDLRRQLR
                     RHGIAAHDRVAIVLPNGPEMAAAFVAIAQSATTAPLNPAYRQEEFAFYLDDLRAKAVV
                     VAAGAGSPAIAAAEARGIAVLRLEGQTDGPAGSFTLSGPDQGGIEDSEEPGAGSTALI
                     LHTSGTTSRPKIVPLLQSNIVASARHIAATLALTPADRCLNVMPLFHIHGLIAAVSAS
                     LSAGASIWCAPGFDALKFFGWMKEARPSWYTAVPTMHQAILSRAARNQDAIAAMPLRF
                     LRSSSASLPAQVMLSLAETFKAPVIEAYGMTEAAHQMTSNPLPPRPQKPGSVGVAAGP
                     LVRVAHEVENRLIDGTGEVVISGANVTPGYEGNPDANAKSFFEAEGHRWFRTGDQGAF
                     DGDGYLHLTGRLKEIINRGGEKISPLEVDGVLMDHPAIAQVVAFAVPHPKLGEEVAAA
                     VVLRDGHMATAREIRDFAAGRMADFKVPRHVVILAEIPKGATGKLQRIGLAEKLGLAA
                     RDAPEVQQQPNADRGSP"
     gene            3091..3360
                     /locus_tag="AC16A0_09825"
     CDS             3091..3360
                     /locus_tag="AC16A0_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105675.1"
                     /translation="MTMLTTCHTLSTNAGRSWVKGLTLTLLATAAILAAASSADAKRS
                     AADQALYEKARKECNNVRKYPDGARPIINYKGGWFRCQEPKFRRD"
BASE COUNT          691 a         1143 c         1080 g          599 t
ORIGIN      
        1 tcggccaaca acgaaaaccg caatcaagtg gtgtccgata tccggcagaa aacccggttg
       61 ccgggcggag aatcattcat tcgcgttgca attgcaagcg gtccgggcgc agggtaacag
      121 taggcgggaa accgggaagc cttgcaaggc accttgcgca gaggaacgcc ttgctcacgc
      181 caataacctc aacattacag atatttgaat tgtcatcctg ctgacccgac attcgcagtg
      241 ctgcggcccg gagccaaggt cctgccgcag ggcctcgtcc ggagctgcac cgctcaggac
      301 gccgcaccct tgcggcggga gacggatcgg ccggtgcgtg aaccggggga tcagcatcac
      361 ttgccgccaa acttcgcgtt cggctaaact ggatcgatgc caaatcaggc ccgcgctagc
      421 gcaggttcgc ttgaagatgg cccagctcac attactgcaa acacaaagca cttgatcgca
      481 tgaaacgcct ttttccgtgg ctcggccagc tcaatcgaag aacgacagag gcctcagccg
      541 actctgacgc agcgtcggct tgccgattca atctcgcatc ctacaacatc cacaagtgcg
      601 tcgggaccga tggacggttt gacccgcagc gcatcaagca ggtcatcctc gcgctcgacg
      661 ccgatatcgt ggtgctgcag gaggcggatg cacggtttgg cgagcgggaa ggacttctcg
      721 acctgtccta tctccatgcc gccggcggat accagtccgc cgttgccctg tccgagggct
      781 cacgcagcca cggctggcat ggcaatgtcg tgctctatcg cagcggcatc gtccacgacg
      841 ttcatcagct gacactgcca gggctcgaac cgcgcggcgc ggtgctggtg gatttcggca
      901 tcgagcaagc caattttcgg ctgcttgcgg tgcatttcgg tctcctgcgc cagtcgcgaa
      961 tgcgacaggt tcaggcgatc atcgatgcgg cggatccgca cccggaccgc catgtcatca
     1021 tcgttggcga catgaacgag tggcgcttga agaaccgctc tgcccttgat ctcttcaagc
     1081 atcatttcga cgaagtgtcg cagaacctgc cgagctaccc ttcgcgcctt ccactgctgc
     1141 cgctcgaccg ccttttcatc agccgcggcc tgcgcagcga aggcatgagc gtgatcgaca
     1201 cgcccctgac acggcttgcc tcggaccacc tgcccctcac ggcgaagctg tcctttcagg
     1261 accggttcta gcgccgggaa ggaaccgccg ggagtcactg gaccaaggcg cggggccggc
     1321 gatcaggatc aggcccgcca tcaccacctc cgaacgcctt gccattcccg aaggcagggc
     1381 ttcatactga gcggagccaa tccggcgcag gctcaattct ttcgatcgaa ggacgggacc
     1441 gggacatgag tgcgacgctt gcaggtctgc tggccgacaa ggatgcggag tcacccgcca
     1501 ttggagcgcc gggccggcca tggctgacct atggcggatt gcgaagcctg tccctggatc
     1561 tccgccggca gctccgccgt cacggcattg cagcccatga ccgggttgcc atcgtgctgc
     1621 cgaacggccc ggagatggcc gcagccttcg tcgccatcgc ccagagcgcg accacggcac
     1681 ccctcaatcc cgcctaccgg caggaggagt tcgcattcta tctcgatgac ctgagggcaa
     1741 aggccgtggt cgtggccgcc ggtgccggga gcccggccat tgccgccgcc gaggctcggg
     1801 gcatcgccgt cctgcgcctc gaaggccaga ccgatgggcc ggctggatca ttcacactat
     1861 cgggaccgga ccaaggcgga atcgaagaca gcgaggagcc gggcgccggg agcacggcgc
     1921 tcatcctcca tacatccggc accacgtccc gccccaagat cgtgccgctg ctgcagtcaa
     1981 acatcgtagc atccgcccgg cacatcgctg cgactcttgc gctgactcct gccgaccgtt
     2041 gtctcaacgt catgccgctg ttccacattc atggcctgat tgcggccgta tcggcatcgc
     2101 tcagcgccgg agcctcgatc tggtgcgcac cgggcttcga tgcgctgaaa tttttcggct
     2161 ggatgaagga ggcacggccc agctggtaca cggcggtacc gaccatgcac caggccatcc
     2221 tgagccgcgc cgcgcgcaat caggatgcga tcgctgccat gccattgcgc ttcctgcgct
     2281 cttcttcggc ctcgcttccg gcacaggtga tgctatccct ggccgagacc ttcaaggcgc
     2341 cggtgatcga agcctatggc atgaccgaag cagcccacca gatgacgagc aatcccttgc
     2401 cgccgcgccc ccagaagccg ggttcggtgg gggtcgcagc cggcccgctg gtgcgggtcg
     2461 cccatgaggt ggagaaccgg ctgatcgacg ggaccggcga ggtggtgatc tcgggcgcca
     2521 acgtcacgcc aggctatgag gggaatcccg acgccaatgc gaagagcttc ttcgaggcgg
     2581 aagggcatcg ctggttccgc accggtgacc agggtgcctt tgacggtgac ggctatctgc
     2641 atctcaccgg acggctcaag gagatcatca atcgtggtgg cgagaagatc agcccgctcg
     2701 aagttgatgg cgtgctgatg gaccaccctg ccatcgcgca ggtggtggcc ttcgcggtac
     2761 cgcacccgaa gctgggcgag gaggttgcag cagcggtcgt tctgcgtgac ggacacatgg
     2821 ccaccgcgcg cgagatccgc gactttgccg ccgggcgcat ggccgacttc aaggtgccgc
     2881 gccatgtcgt catcctcgcc gagattccga aaggagcaac cggcaagctg cagcggatcg
     2941 gccttgccga gaagctaggc ctcgccgccc gggacgcgcc cgaggttcag caacagccga
     3001 acgcggaccg aggatctccc tgacacccac tgatcgatcc gatcagtgaa gaagagactc
     3061 gagaacagac atgaaaggtg attgaagcgc atgacgatgc tcacaacatg ccacacgctg
     3121 tcgacgaacg ccggcagatc ttgggtaaag ggcctgacgc tgacgctgct tgcgacagca
     3181 gccatcctcg cagccgcctc ttccgcagat gcaaagcgct cggccgccga tcaggcgctt
     3241 tacgaaaagg cccgcaagga gtgcaacaac gtccgcaagt atcctgacgg agcccggccg
     3301 atcatcaact acaagggcgg ctggttcaga tgccaggagc ccaagttcag acgggactga
     3361 agcttgcagg gcactgcctg cgcgtcagaa atagacgacc acgtagccgc tgcccgagcg
     3421 ctgataatag gtgccgccgc agtagtagag gctatagcca tagtagttgc catagggaca
     3481 gcccgcagga atggcgccaa tataggcccc gcg
//
LOCUS       DBNGVR010000463         2784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2136824, whole genome shotgun sequence.
ACCESSION   DBNGVR010000463 DBNGVR010000000
VERSION     DBNGVR010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2136824"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="AC16A0_09830"
     CDS             complement(<1..502)
                     /locus_tag="AC16A0_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3105676.1"
                     /translation="MTSVKKTFAAVLALGACLAAPAYAGDAESCKAVRFADVGWTDIQ
                     VTTGAASVVLEALGYTPEVKTLSVPVTYASLKNKDIDAFLGNWMPSMTADIQPYLDDK
                     SVEQLATNLEGAGYGLVVPQYVADAGVESLADLGANAEKFGGKIYGIEAGNDGNRIIQ
                     TMIDDAK"
     gene            650..1267
                     /gene="betI"
                     /locus_tag="AC16A0_09835"
     CDS             650..1267
                     /gene="betI"
                     /locus_tag="AC16A0_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HIR3105677.1"
                     /translation="MPKLGMAPIRRKQLIGAAIAVIHEDGFSHATVARIARRAGVSSG
                     MVHHYFKDKDELLFATMRHLLSDLHADAVLRLSAAPSPEARVRAIIDACFGDAQFDAQ
                     VFSAWLALYGSARQSSSLQRILSIYHRRLRSSLLHDLKKMVSAADAARLADGIAAMID
                     GLWLRYALTGQPEDPETPRALTRDYFAAAHDQAFNRRDKSLRVAS"
     gene            1287..2276
                     /locus_tag="AC16A0_09840"
     CDS             1287..2276
                     /locus_tag="AC16A0_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3105678.1"
                     /translation="MKPVKWGIISTANIGVAKVIPGMLKSKDLEVVAIASRKLKPARE
                     WAGKLGIPKAYGSYEEMLADPEIEAVYNPLPNHLHVPLTLAAARAGKHVLCEKPIAIT
                     AKEAQQLRKAPGSVHIAEAFMVRHATQWLAARDLVKKGRIGDVRAIQVFFSYFNRDPK
                     NVRNMADIGGGGLLDIGCYPITVSRFIFNAEPKRVTATFDRDPKFRTDRTAGGLADFG
                     DGRHLSFSVSTQLAPYQRVHIAGTKGRIEIEIPFNAPPDRPNRYFVQGMDMNVGEWIE
                     LPVSDQYQLQAEAFGRIIRGKQKPVWGVGDAIQNMKIIDAFFRSEKSGRWEKP"
     gene            2451..2717
                     /gene="rpsT"
                     /locus_tag="AC16A0_09845"
     CDS             2451..2717
                     /gene="rpsT"
                     /locus_tag="AC16A0_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR3105679.1"
                     /translation="MANTKSAKKATRVAARRTEVNKNRVSRIRTQVRKVEEAIASGDK
                     ATAEAALKAAQPELMRGAQKGVMHKNTASRKVSRLSARVKALKA"
BASE COUNT          545 a          894 c          850 g          495 t
ORIGIN      
        1 tcttcgcatc atcgatcatt gtctgaatga tgcggttgcc gtcattgccg gcctcgatgc
       61 cgtagatctt tccgccgaac ttctcggcat ttgcgccaag atcggccaat gactccaccc
      121 cggcgtccgc cacatattgc gggaccacaa ggccgtagcc cgcaccttca agattggtcg
      181 ccagctgctc taccgacttg tcatccagat agggctggat atccgccgtc atgctgggca
      241 tccagttgcc gaggaaggcg tcgatgtcct tgttcttcag cgaggcgtat gtcaccggca
      301 ccgagagcgt cttcacttcc ggcgtgtagc ccagggcttc cagcaccacg ctcgcagcgc
      361 cggtggtgac ctgaatgtcg gtccagccca catcggcgaa gcggacggcc ttgcagctct
      421 cggcgtcgcc ggcataggcc ggtgcagcga ggcaggcgcc gagcgcgagt acggccgcaa
      481 atgtcttttt cacagaagtc attccagttc tctccctgtt gtgccggagg actgtgtctg
      541 ccgtcctctc ggttctggct ggacactatg cagtttgggc acggctcgca agatttttat
      601 tggacggacg tccaataaag aactagcctg acgcccagga ggagcacgca tgcccaagct
      661 tgggatggca ccgatccgca ggaaacaact gatcggcgcc gccatcgcgg tgatccacga
      721 agatggcttc agccacgcca ccgtggcacg cattgcccgg cgcgcaggcg tctcgtccgg
      781 catggtgcac cattacttca aggacaagga cgaactgctc ttcgccacca tgcgccatct
      841 cctgtcggac ttgcacgcgg atgcggtgtt acggctttcg gcagccccat cgcccgaagc
      901 ccgggtgcgg gcgatcatcg acgcctgctt tggcgatgcg caattcgatg cgcaggtttt
      961 cagcgcctgg ctggcgctct atggcagcgc ccggcagtcc agcagcctgc aacggattct
     1021 cagcatctat caccggcgcc tgcgcagcag cctgcttcac gatctcaaga agatggtgag
     1081 cgcggcggat gcggcccggc tggccgacgg catcgcggcg atgatcgacg ggctctggct
     1141 gcgctatgcc ttgactggcc agcccgagga tccggaaacg ccccgggccc tgacgcgcga
     1201 ctacttcgcc gccgcccatg atcaggcttt caaccggcgc gacaagtcct taagagtagc
     1261 gtcctgattt tccggaggga cgcgcaatga aaccagtgaa gtggggcatc atctcgacgg
     1321 ccaacatcgg cgtggccaag gtcattccgg gcatgctgaa gagcaaggat ctcgaggtcg
     1381 tggcgatcgc gtcgcgcaag ctcaagccgg cccgggaatg ggcaggcaaa ctcggcattc
     1441 cgaaagccta tgggtcctac gaggagatgc tggccgatcc cgagatcgag gccgtgtaca
     1501 atcccctgcc gaaccacctg catgttcctc tgacgctggc agcggcacgc gcgggcaagc
     1561 atgtgctctg cgagaagccc atcgccatca cggccaagga agcccagcag ctcaggaagg
     1621 cgcccggatc ggttcatatt gccgaagcct tcatggtgcg ccacgccacc caatggcttg
     1681 cagcccgcga cctggtgaag aagggcagga tcggcgacgt ccgcgccatc caggtcttct
     1741 tctcctactt caatcgtgac cccaagaacg tgcgcaacat ggccgacatc ggcggtggcg
     1801 gcctgcttga catcggctgc tacccgatca ccgtgtcgcg tttcatcttc aatgccgaac
     1861 cgaagcgggt cacggccacc ttcgatcgcg atccgaaatt caggaccgac cgcaccgccg
     1921 gcggactggc cgatttcggc gatggacggc acctgtcctt ctcggtttcg acgcagctgg
     1981 cgccctatca acgcgtccat attgccggaa ccaagggacg catcgagatc gagatcccct
     2041 tcaacgcgcc gccggaccgg ccgaaccgct actttgtcca gggcatggac atgaacgtgg
     2101 gcgagtggat cgaactgccg gtttcggacc agtaccagct tcaggccgag gccttcggcc
     2161 ggatcatccg cgggaagcag aaaccggtct ggggcgtcgg cgacgccatc cagaacatga
     2221 agatcatcga cgcctttttc cggtccgaga agagcggaag gtgggagaaa ccctgatccg
     2281 gaagggcggc ggaccggcca gacaagggaa ttgacgtccc tgccgacccc gactataagc
     2341 cgcccattcc cgatgggccg ggaccaaggg ccgcatgctg caaggtgtgc ggccatgggt
     2401 atttgagggc ccgttgcaac gacatcacga gacaagcagg accaaactct atggccaata
     2461 cgaaatcggc gaagaaggcc acccgcgttg ccgcccgccg caccgaagtc aacaagaacc
     2521 gcgtcagccg catccggacc caggtgcgca aggtcgaaga agcgatcgcc tctggcgaca
     2581 aggcgaccgc cgaggcggcg ctgaaggcgg cgcagcccga actcatgcgc ggtgcccaga
     2641 agggcgtcat gcacaagaac acggcatcgc gcaaggtttc gcgcctgagc gcgcgcgtca
     2701 aggctctgaa agcctgatcg ttttttcagg tcccgaagtt tagcgcgccg gaggccagcc
     2761 tccggcgcgt tttgtttgtc aggc
//
LOCUS       DBNGVR010000464         4705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_565255, whole genome shotgun sequence.
ACCESSION   DBNGVR010000464 DBNGVR010000000
VERSION     DBNGVR010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_565255"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16A0_09850"
     CDS             complement(<1..200)
                     /locus_tag="AC16A0_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105680.1"
                     /translation="MAFLGFGVSRVLKAVAASGVVMGMLAMAAVPPGAFAGSGEPAHK
                     LLKKSSKQASAPVKQNWSSGGNT"
     gene            complement(451..951)
                     /gene="ldtR"
                     /locus_tag="AC16A0_09855"
     CDS             complement(451..951)
                     /gene="ldtR"
                     /locus_tag="AC16A0_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LdtR"
                     /protein_id="HIR3105681.1"
                     /translation="MGALRPVPAEPVENPIKPRYLEALTLIERLHRRLLDVIKDDFER
                     SGEPEVNPVQALLLFNIADAELTAGELKTRGHYQGSNVSYNLKKLVESGYVHHERSAT
                     DKRSVRVRLTDKGRAIRERVNALYNRQLQAVEQVVGLNTDELDRLNKALTRLERYWTD
                     QILYRL"
     gene            1243..2019
                     /locus_tag="AC16A0_09860"
     CDS             1243..2019
                     /locus_tag="AC16A0_09860"
                     /inference="COORDINATES: protein motif:HMM:NF018597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin-like domain-containing protein"
                     /protein_id="HIR3105682.1"
                     /translation="MNIPSLLLISTMLLATCGDVRAQAIPIPRPKPETATTSNAVPVP
                     RPKPSQPAQAVDEAAPDTPAVWTETQVADALAACKTQLAGLDIAYQPIASLGGPDGCG
                     AAAPVELTRVADVDIVPPAKVTCSMAKQLFLWVSQSVQPEAQSRLGTCVTAIRNAASY
                     VCRGRNNQKGGKLSEHGRANALDMSGFSFGKTKDISIGDGWGETPVIGVSSKGSFLEA
                     VRSGACKQFATVLGPGSDAYHGDHFHVDAIQRKNDYRICQ"
     gene            2074..3075
                     /gene="hemB"
                     /locus_tag="AC16A0_09865"
     CDS             2074..3075
                     /gene="hemB"
                     /locus_tag="AC16A0_09865"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3105683.1"
                     /translation="MTELPMPSFPHLRMRRNRRTAWSRDLVRENSLQVSDLIWPVFVI
                     EGKDTAVPVPSMPGVERHTVDRLVRKAEQAAELGIPAIALFPNTPSSLRSADAREAFN
                     PDNLVCQAVRAVKDAVPDIGIMCDVALDPYTSHGHDGLMDGGEILNDATIEALVKQSL
                     VQAQAGCDIIAPSDMMDGRIAAIRAALEGNRLQHVQIMAYSAKYASGFYGPFRDAVGS
                     SGTLVGDKRTYQMDPGNTDEAIREVALDIAEGADMVMVKPGMPYLDIVQRVKATFRVP
                     TYAYQVSGEYAMIMAACQNGWLDPNKVISESLMAFKRAGADGILTYFALDVARQLKG"
     gene            complement(3072..3950)
                     /locus_tag="AC16A0_09870"
     CDS             complement(3072..3950)
                     /locus_tag="AC16A0_09870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3105684.1"
                     /translation="MTRVAWIGLGVMGFPMAGHLAKRGGFDVTVYNRTAAKAARWVEA
                     YGGRAAATPRAAAEEADVVFACVGNDDDLRQVAVGPEGAFQAMKRGALFVDHTTASAT
                     VARELHVLAKAQGLGFVDAPVSGGQAGAENGVLTVMCGGDPVDYDRAEPLIAHFSRMR
                     KLLGPAGSGQLAKMCNQIAIAGLVQGLAEAVNFGMKAGLDMLAVVDVISKGAAGSWQM
                     ENRHKTMIEGKFDFGFAVDWMRKDLSICLEESRRNGAPLPVTALVDQFYAEVQKLGGG
                     RWDTSSLIARFQRLDR"
     gene            4086..>4705
                     /locus_tag="AC16A0_09875"
     CDS             4086..>4705
                     /locus_tag="AC16A0_09875"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR3105685.1"
                     /translation="MSIERRNFPQFFITAPSPCPYLDGKVERKVFTHLVGQDARSLNT
                     QLSQGGFRRSQNIAYRPACEGCAACVSVRVPVNTFVPGRAFRRNLRANSDIESMVVRP
                     LATREHYSLFRAYIDERHGDGGMADMTALDFSAMVDDNFVDSRLVEYRVRTTRAEDSL
                     VAAVLIDILGDGVSMIYSFYDPDQARRGLGTFMILDNIQRVRRLGLP"
BASE COUNT          895 a         1502 c         1405 g          903 t
ORIGIN      
        1 gtgttgccgc ccgaagacca gttctgcttc accggcgcag aggcctgttt cgaagacttt
       61 ttcaggagct tgtgggcagg ttcaccgctg ccggcgaaag ctcccggcgg aacggcggcc
      121 atggccagca ttcccatgac cacgcccgaa gcggcaaccg ctttcagaac ccgcgaaacc
      181 ccaaacccaa gaaaagccat ccgcaatcct cttcagccga cctcgtgccg taaggccaca
      241 cctaactccc cgacctgcag cccttgtggg gtcttatcag ccccgcttcg actcgtcaaa
      301 actccgccac aagtcagcca ccgtatctaa cggcaattga ggcgacactg tgggagcttg
      361 gcgcttctgg ggttgggata cggcaaaaaa ccgaacgctg cagctttttg gccacagcgt
      421 ccggtcgtcg gttgggtgcg caggcgccga tcagaggcgg tagaggatct ggtcggtcca
      481 gtagcgttcg aggcgggtca gggccttgtt gagccggtcc agttcatcgg tgttgagacc
      541 cacgacctgc tccaccgcct gaagctggcg attgtacagc gcgttgactc gctcgcggat
      601 ggcgcggccc ttgtcggtca ggcgcacgcg caccgagcgc ttgtcggtgg ccgagcgttc
      661 gtgatggaca tagccggatt cgaccagttt cttgagattg taggagacat tggagccctg
      721 atagtgaccg cgggtcttca gctctccggc tgtcagctcg gcgtcggcaa tgttgaacag
      781 gagcagggcc tgaaccgggt tcacctcggg ctccccgctc cgctcgaagt catccttgat
      841 cacgtcaagc agccggcgat gcaggcgctc gatcagcgtg agcgcttcga ggtaccgcgg
      901 cttgatcgga ttctccaccg gctcggccgg cacggggcgc aatgccccca tcgtttccac
      961 acgcatgtta tccacctttt cccttgtcgg acagggccag ctcttgttgg cagactgatc
     1021 cccccaatgc gtgtgaccct acggccgagg gacttaagat cggttcaagg ggttcggtaa
     1081 atttgtcctt atattttcaa ttgtttgagc cgagttcgcc tagagtttat ggagtctttt
     1141 cggacgccct taacggggtc tgagacaatg cgcttaatgg cctctgaacg tgtgccggac
     1201 gggtcgcttg ccaagccgag aagagctggg tgtaatgggt tgatgaacat cccgtccctg
     1261 cttctgatct ccaccatgct gttggcgaca tgcggcgacg tccgggcgca ggccatcccg
     1321 attccacggc cgaagccgga gacggcaacg accagcaatg ccgttcccgt tccgcggcca
     1381 aagcccagtc agcccgcaca ggctgttgac gaagcggcac cggatacgcc ggcggtctgg
     1441 accgaaacgc aagtggcaga cgccctggct gcctgcaaga cgcagcttgc gggcctggat
     1501 attgcctacc aacccatcgc cagcctgggc ggtcccgatg gttgtggcgc agcggcaccg
     1561 gtggaattga cacgcgtggc ggatgtcgac atcgtcccgc cggcgaaagt cacctgcagc
     1621 atggcgaagc agctctttct gtgggtcagc cagtcggtcc agcccgaagc gcagtccagg
     1681 ctcggcacct gcgtgaccgc catccgcaac gccgcttcct atgtctgccg cggtcgaaac
     1741 aaccagaagg gcggcaagct gagcgagcat ggccgggcca atgcgctcga catgtcgggc
     1801 ttcagcttcg gcaagaccaa ggacatttca atcggcgacg gctgggggga aacgcccgtg
     1861 attggcgttt ccagcaaggg gtccttcctg gaggctgtcc gcagcggcgc ctgcaagcag
     1921 ttcgccaccg tgcttggacc tggctccgat gcctatcatg gcgaccactt ccatgtggac
     1981 gcaatccagc gcaagaatga ttaccgcatc tgccaatgag agggctgcaa ggtgcccttc
     2041 agcgagatgc caccgacccg ttacggagtg accatgactg aattgccgat gccgtccttt
     2101 ccccatctcc gcatgcgccg caatcggcgg acggcctggt ctcgcgatct ggtgcgcgag
     2161 aacagcctgc aggtgtccga cctgatctgg cccgtgttcg tcatcgaggg aaaggacaca
     2221 gccgttccgg tgccgtcgat gccgggcgtg gaacgccaca ccgtcgaccg ccttgtccgc
     2281 aaggcggagc aggccgccga acttggcata ccggcgatcg ccctgttccc gaacacgcca
     2341 tcatcgctgc gcagcgccga tgcccgcgaa gccttcaatc ccgacaatct ggtttgccag
     2401 gcggtgcggg ccgtcaagga tgccgtgcct gacatcggca tcatgtgcga cgtggctctc
     2461 gatccctata caagccatgg ccatgacggc ttgatggacg gcggcgaaat tctcaatgac
     2521 gccaccatcg aggccctggt caagcagtcg ctggtgcagg cccaggccgg ctgcgacatc
     2581 attgcgccat cggacatgat ggatggccgg atcgccgcca tccgggccgc actcgaaggc
     2641 aaccggctgc agcatgtcca gatcatggcc tacagcgcca agtatgcatc cggattctat
     2701 ggacccttcc gcgatgccgt ggggtcttcc ggcacgctgg tgggcgacaa gcgcacgtat
     2761 cagatggatc ccggcaacac cgacgaggcc attcgcgagg tggcgctcga catcgccgaa
     2821 ggtgccgaca tggtgatggt caagcccgga atgccctatc tcgatattgt ccagcgggtg
     2881 aaggcaacgt tccgtgtacc gacctatgcc tatcaggtgt cgggagagta tgcgatgatc
     2941 atggcggcct gccagaatgg ctggctggat cccaataagg tgatctccga aagcctgatg
     3001 gccttcaagc gcgccggcgc ggatggaatc ctcacttact tcgccctcga tgtggcgagg
     3061 cagctcaagg gctagcggtc gagcctctgg aagcgcgcga tgagactgga cgtatcccag
     3121 cggccgccac cgagcttctg cacttccgcg tagaactgat ccaccagtgc ggtcaccgga
     3181 aggggcgcgc cgttgcgccg cgattcctcc aggcagatgg aaaggtcctt gcgcatccag
     3241 tcgacggcaa aaccgaagtc gaactttcct tcaatcatcg tcttgtggcg gttctccatc
     3301 tgccaggatc cggcagcgcc ctttgagatc acgtcgacca cggccaacat gtcgagaccg
     3361 gccttcatcc cgaagttgac ggcctctgcc aatccctgca cgagccctgc aatggcgatc
     3421 tggttgcaca tcttggccag ctgaccagat cccgcaggac ccagcagctt ccgcatgcgt
     3481 gaaaaatgcg cgatcagggg ctcggcccgg tcatagtcaa ccggatctcc accgcacatg
     3541 acggtcagga cgccattctc ggcaccggcc tgccctccgg acactggcgc atccacaaaa
     3601 cccaagccct gtgccttggc gaggacatgg agttcgcgtg cgactgtcgc cgatgccgtc
     3661 gtatgatcaa caaagagagc gcctcgcttc atggcctgga aggcgccttc cggtccaacg
     3721 gccacctgtc ggaggtcgtc atcgttgccc acacaggcga aaacaacatc tgcttcctct
     3781 gctgccgcgc gcggcgtcgc ggccgccctg ccgccatatg cctccaccca tctggcggcc
     3841 ttggcagccg tccggttata gactgtcacg tcgaaaccgc cgcgcttcgc aaggtgtccg
     3901 gccatcggaa agcccatcac cccaagtccg atccaggcca cccttgtcat cacgaaatct
     3961 cctgctgcgc cttcaatgac ttggttagga catcatgcag gagaattcaa actgccgaat
     4021 tcccttgaat gaagggtgag aggcggcatt atatccgcac gcaaacgtgg agccggaatc
     4081 cgaccgtgag catcgaacgg cgcaacttcc cccagttttt cataaccgcg ccttcgccct
     4141 gcccctacct ggacggcaag gtcgaacgga aagtgttcac tcacctcgtc gggcaggatg
     4201 cgcgctccct caacacgcag ctctcgcagg gcggcttccg acgcagccag aacatcgcct
     4261 atcggccggc gtgcgaaggc tgcgcggcat gcgtttcggt ccgcgtgccg gtcaacacct
     4321 ttgtgcctgg ccgggccttc cggcgcaatc tgcgggccaa ttcagacatc gaaagcatgg
     4381 tggtcagacc gctggcgaca cgcgagcatt acagtctctt ccgagcctat atcgacgaac
     4441 gccatggcga tggtggcatg gcagacatga cggcattgga tttctcggcc atggtcgacg
     4501 acaatttcgt cgacagccgg ctggtggaat accgggtccg gactacaagg gcggaggata
     4561 gcctcgttgc cgccgttctc atcgacatcc tcggcgacgg cgtgtcgatg atctacagct
     4621 tctatgatcc tgatcaggcg cggcgcggtc tcgggacttt catgattctc gacaatattc
     4681 agcgggtccg gcggcttggt ctgcc
//
LOCUS       DBNGVR010000465         2971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3317984, whole genome shotgun sequence.
ACCESSION   DBNGVR010000465 DBNGVR010000000
VERSION     DBNGVR010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3317984"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="AC16A0_09880"
     CDS             complement(<1..749)
                     /locus_tag="AC16A0_09880"
                     /inference="COORDINATES: protein motif:HMM:NF037123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF11 domain-containing protein"
                     /protein_id="HIR3105686.1"
                     /translation="MLYVDIPSRPEIEKLASSRAEPSVTIYVKTTPLTQEIGASRIAL
                     KNLEHQALEQLKAAGVDKRRLWPIEEVINHLVDDDAFWATQANSLALFATPDGLLTYR
                     LANHIPDQVHVSDRFHIQPLLRAVTFPQSAFILVLGENGVKLYQLDADTEPQPVRVPD
                     LPKDAGDALGKSTLNDRSPSGRIHGSEGQNFRLRQYARIVDQALRHVLTGRDRPLIIA
                     ATDPLASMFRQANTYPHLAPGEIKGNSERLAP"
     gene            908..1336
                     /locus_tag="AC16A0_09885"
     CDS             908..1336
                     /locus_tag="AC16A0_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ohr family peroxiredoxin"
                     /protein_id="HIR3105687.1"
                     /translation="MKILYTAHATSVGGRGGGTAKTADGALDVKLDHPVEMGGKGDGV
                     NPEQLFAIGYASCFLGAMKFVASKMTPPLKLAPESSTTAHVSFGARDDGEGFGLEVVM
                     DVNLPGLDKAAAKDLAAKAHVVCPYSHATKGNIKVTTNIV"
     gene            complement(1341..1886)
                     /locus_tag="AC16A0_09890"
     CDS             complement(1341..1886)
                     /locus_tag="AC16A0_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3105688.1"
                     /translation="MLKPVMRTFAAVGFLAVALTGSSWADDDGGNWWPRWGMGQGMMG
                     GGGWHDSMMGYGPEAMLDRIDGRLAFLKTELKITEAQSAAWDELAGVIRTTSEAHNDM
                     MRSMMKDMRSGEFLKAPLPERLQIQQTRLEARLDQVKSVKAAVEKLYAMLDDGQKRAA
                     DDIVLPMMGMGMGRGMMMRDD"
     gene            2103..>2971
                     /locus_tag="AC16A0_09895"
     CDS             2103..>2971
                     /locus_tag="AC16A0_09895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3105689.1"
                     /translation="MTSQDPLVAAATRFAAHLGKVLDVPAAIRLWDGTIVPLGSGDHG
                     GRMITIAGPGVLGTLVRKPSLDTIFRLYVTGDIAYEGTNLIELMDLLRQRLARRKIDL
                     ADLRKGLPLGAVLRFLPVRADAGVKHAYQGDEVASGGAARDNKAFVQFHYDVSNDFYA
                     LFLDREMVYSCAYFTDWSNGIDQAQRDKLDLICRKLRLKPGERFLDIGCGWGALLCHA
                     AANYGVKAYGVTLSQAQFDFATAKVKRLGLEDRVTIELKDYNLIEGTFDKIASVGMYE
                     HVGTAHYDVYFRK"
BASE COUNT          544 a          953 c          957 g          517 t
ORIGIN      
        1 ggggcaagac gttcggaatt gcccttgatc tcgcccggcg ccagatgcgg ataggtattg
       61 gcctggcgga acatggaggc gagcggatcg gtggcggcga tgatcagcgg ccggtcgcgg
      121 cccgtcagca catggcgcag cgcctgatcg acgatgcggg catattgccg gagacggaag
      181 ttctggcctt ccgagccgtg gatgcggccc gagggcgagc ggtcgttcag cgtggacttg
      241 ccgagcgcgt cgccggcgtc cttgggcaga tcgggcacgc gcaccggctg gggctcggtg
      301 tcggcgtcaa gctgatagag cttcacgccg ttctcgccga gtaccagaat gaaggcactc
      361 tgcggaaagg tgacggcgcg cagcagcggc tggatgtgga agcggtccga cacatgcacc
      421 tgatcgggaa tgtggttggc gagccggtag gtcagcaggc cgtcgggcgt ggcgaagagc
      481 gccaggctgt tggcctgggt ggcccagaag gcatcgtcat cgacaaggtg gttgatcacc
      541 tcctcgatcg gccacagccg gcgcttgtcg acgcccgcgg ccttcagctg ttccagcgcc
      601 tggtgctcga ggtttttgag cgcgatacgg ctggcgccga tctcctgcgt gagcggcgtg
      661 gtcttgacgt agatcgtcac cgacggctcg gcccgcgacg aggccagctt ctcgatctcg
      721 gggcgcgacg ggatatcgac gtaaagcatg aactactcct gatgatggaa cgtagacggt
      781 cacggttcgg gcgggggact atggcggggt tgccgcgcca tcacaagggg ctgaggttca
      841 acggccttga tcatccggcg aagccactcc acaatcgacc gcgacagcca acagggagaa
      901 cgcgacgatg aagatcctct acaccgcaca tgcaacctcg gtcggcggac gcggcggcgg
      961 cacggccaag accgccgatg gcgcgctcga cgtcaagctc gaccacccgg tggaaatggg
     1021 cggcaagggc gacggcgtca acccggaaca gctcttcgcc atcggctatg caagctgctt
     1081 cctcggcgcc atgaaattcg tggccagcaa gatgacgccg ccgctgaagc tggcgcccga
     1141 atcctccacc accgcgcatg tgagcttcgg ggcgcgcgac gatggcgaag gcttcggcct
     1201 tgaagtggtc atggatgtga acctgcccgg cctcgacaag gcagcggcca aggaccttgc
     1261 cgccaaggcc catgtggtgt gcccctattc ccacgccaca aagggcaaca tcaaggtgac
     1321 gacgaacatc gtctgatctg tcagtcatcc cgcatcatca ttccgcgtcc catgcccatg
     1381 cccatcatgg gcaggacgat gtcatcagcg gcccgcttct gaccatcgtc cagcatggca
     1441 tagagtttct ccacggccgc cttcacgctc ttgacctggt cgagacgtgc ttcaagccgc
     1501 gtctgctgaa tctgcaggcg ttccggcaac ggtgccttga gaaactctcc actgcgcatg
     1561 tccttcatca tgctgcgcat catgtcgttg tgggcttcgc tggtggttcg aatgacaccg
     1621 gcaagctcat cccaggcggc agactgcgcc tcggtaatct tcagttccgt cttgaggaag
     1681 gcgagccgtc cgtcgatcct gtcgagcatg gcctcggggc catagcccat catcgagtca
     1741 tgccagcccc cgcctcccat catgccctga cccatgcccc agcggggcca ccaattgccg
     1801 ccgtcatcat ccgcccagga tgacccggtc agcgcgaccg caaggaagcc gactgctgcg
     1861 aaagtcctca tgactggctt caacatggca tcatctcctg ttccatggtt gttttctgct
     1921 ttaaaacctc atgatcgcgc gaagccgcga cttcggcctt gatccatcac aaggacgaag
     1981 aagggggcag ccgcgcgctg ccgcgccgca gagcggccat gcgcggggcg aaaggccccg
     2041 gccgcaagct ctggccaagg cgcaaccgaa tggctagggt cgcccgcgaa acgggggacc
     2101 ggatgacatc gcaagaccca ctcgtcgcgg cggccacgcg gtttgccgcg catctcggca
     2161 aggtgctgga cgtgccggcc gccatcaggc tgtgggacgg caccatcgtt ccgctgggga
     2221 gcggcgacca cggcgggcgg atgatcacca tcgcggggcc cggcgtgctc ggcacgctgg
     2281 tgcgcaagcc cagcctcgac acgatattcc ggctctatgt caccggcgac atcgcctatg
     2341 agggcaccaa tctgatcgaa ctcatggacc tgctgcgcca gcgcctggcc cgcaggaaga
     2401 tcgacctcgc cgacctgcgc aagggcctgc cgctgggcgc ggtgctgcgc ttcctgccgg
     2461 tgcgcgcgga tgccggcgtg aaacacgcct accagggcga cgaggtggcg tcaggcggcg
     2521 cggcgcgcga caacaaggct ttcgtgcagt tccactatga tgtcagcaac gacttctacg
     2581 ctctcttcct cgaccgtgag atggtctatt cctgcgccta cttcaccgac tggtcgaacg
     2641 gcatcgacca agcccagcgc gacaagctgg atctcatctg ccgcaagctg cggctgaagc
     2701 ccggcgagcg cttcctcgac atcggctgcg gctggggtgc cctgctctgc cacgcggcgg
     2761 cgaactacgg cgtgaaggcc tatggcgtga cgctgtcgca ggcgcagttc gatttcgcca
     2821 ctgccaaggt gaagcgcctc ggactcgaag accgcgtgac gatcgagctg aaggactaca
     2881 atctcatcga agggaccttc gacaagatcg cctcggtggg catgtatgag catgtcggaa
     2941 ccgcacatta cgacgtctac ttccgcaaga t
//
LOCUS       DBNGVR010000466         7029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3710730, whole genome shotgun sequence.
ACCESSION   DBNGVR010000466 DBNGVR010000000
VERSION     DBNGVR010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3710730"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="pnp"
                     /locus_tag="AC16A0_09900"
     CDS             complement(<1..1262)
                     /gene="pnp"
                     /locus_tag="AC16A0_09900"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3105690.1"
                     /translation="MFNIDVEEITWGSATLRLETGRVARQADGAVLASMGETTVLATA
                     VAERSAKPGIDFFPLTVNYQEKTYAAGKIPGGYFKREGRPTERETLISRLIDRPIRPL
                     FVAGFRNETQVIATVLSYDLENDTDILALVASSAALTLSGIPFMGPIGAARVGYINGE
                     YVLNPTVEQQKVSELDLVVAGTQDAVLMVESEAKELSEEIMLGAVMFGHRGFQPVIEA
                     IIRMAERSARSPRDFTPPDEEALYNQVKAIAEAEVRAGYAIPTKEARHEAVAKAKDKV
                     LAALTGEDKPDAPPANKVGEAFKHLEKDVVRNAILDTGLRIDGRDTKTVRKIVAEAGV
                     LPRTHGSALFTRGETQALVVTTLGTGEDEQYIDSLDGTTKGTFLLHYNFPPFSVGETG
                     RMGATGRREIGHGKLAWRAIHPLLPPSD"
     gene            complement(1432..1701)
                     /gene="rpsO"
                     /locus_tag="AC16A0_09905"
     CDS             complement(1432..1701)
                     /gene="rpsO"
                     /locus_tag="AC16A0_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3105691.1"
                     /translation="MTMTAERKVALVKEFATKPGDTGSPEVQVAILTERINYLTDHFK
                     THKKDNHSRRGLLTMVSKRRSLLDYLKRKDEGRYQTLIKRLEIRR"
     gene            complement(1720..2637)
                     /gene="truB"
                     /locus_tag="AC16A0_09910"
     CDS             complement(1720..2637)
                     /gene="truB"
                     /locus_tag="AC16A0_09910"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3105692.1"
                     /translation="MKKKRGSPVHGWVALDKPLGMTSTQAVGKVRWLFGAEKAGHGGT
                     LDPLATGLLPIALGEATKTVSAAMEGVKTYRFTVKWGEERSTDDLEGVVTTCSANRPS
                     LESIESILARFKGEIQQVPPAFSAIKVEGERAYDLARSGADVVLASRAVRIDDLRLIA
                     VCEQDSATFEVTCGKGTYVRSLARDMGRTLGCLGHVTMLRRLAVGALTEAHMIPLEKL
                     EELRHKAPGGNAMDGVLRPIETVLDGIPALAIDEQQAQRLRQGQSVLLRGANAPIAHE
                     AVLITSGGKPLGIGSVEQGTLKPKRLFNL"
     gene            complement(2634..3020)
                     /gene="rbfA"
                     /locus_tag="AC16A0_09915"
     CDS             complement(2634..3020)
                     /gene="rbfA"
                     /locus_tag="AC16A0_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3105693.1"
                     /translation="MKPAAGPSQRQLRAGELIRHALAGILLRGETGDPELERLGVTVH
                     EVQISPDLKIATAYIRTLIAGAEPKALEALNRNRKYIRGLIAPKLDMKFMPDIRFRID
                     TALDYASKIDEILRRPDVARDLKTEE"
     gene            complement(3028..5643)
                     /gene="infB"
                     /locus_tag="AC16A0_09920"
     CDS             complement(3028..5643)
                     /gene="infB"
                     /locus_tag="AC16A0_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3105694.1"
                     /translation="MTDSNDNDRKADGARTGGGTLTLKRPVVEQSRVKQSFAHGRTKT
                     VVVETKRKRMGDDKPAAAESKPAAFQSQPKVAQGASQTATQQRPQAPSGGAGRSGVVL
                     RTLTAEEKEARDRALSDARLREADERKRQEEEARRRSELNERESIEREAAARRKAEDD
                     ARHRVEEEGRRKAEEAAKKLAPKTPAGVVTPATVPMKPSTVTAKRPVNDDDDGPRTAR
                     SGGPAKRELKAPVPTRAKTDADKRRSRLTVVNALTEEEGERGRSVAAFRRRTERLKKQ
                     AQGFQMPTEKVSREVVIPEAITIQELANRMAERAVDIIKLLMKQGQMHTINDVIDADL
                     AQLIAEEMGHKVKRVSESDVVEGLAGDTDDEGSLQSRPPVVTIMGHVDHGKTSLLDAI
                     RKTNVVSGEAGGITQHIGAYQVQTRTGLITFIDTPGHEAFTAMRARGAKATDIVVLVV
                     AADDGVMPQTVEAINHARAADVPIIVAVNKIDKPSANPNKVRTDLLRHEIVVESMGGD
                     TLEVEVSALKGTNLDKLLDTILLQAEVLDLKANPDREAGGLVVEAQLDKGRGPVATVL
                     VQRGTLRLGDIFVAGSAWGRVRALINDKGEQVKEAGPSSPVEVLGLNSAPEAGDQFDV
                     VPNEARAREITDYRDRKRRESRGAASARSSLEQMMSQMKEGGIKEFPILVKADVQGSA
                     EAIVQALEKIGNDEIRARVIHYAVGGINESDIGLAQASGAVVLGFNVRASGQARDAAE
                     RSGIEIRYYNIIYDLVDDIKAAMSGKLAPELRETFLGNARILEIFNITKVGKVAGCQV
                     TEGSVQRGAKVRLIRDNVVIHEGTLSTLKRFKDEVKEVQVGQECGMAFENYQDMRAGD
                     VIECFRVEKVQRSLD"
     gene            complement(5640..6251)
                     /locus_tag="AC16A0_09925"
     CDS             complement(5640..6251)
                     /locus_tag="AC16A0_09925"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIR3105695.1"
                     /translation="MEADSDLATRMCIVTRECLDEAVLLRFVRSPDGVAVPDLARKLP
                     GRGVWVTLNRSRVAEAVKKHLFSKGFSEETRSPDGLPDMVARLLRQNALSTFSLAKKA
                     GEAVTGFMKVEEALARGRVRILCHAADAQPDGCRKLDRMAGDGVMTVRMFSRDELDLA
                     FGRSNVVHAAVTKGGLAEKLVAAVRRIDVFDAPLGSKGSEERV"
     gene            complement(6269..>7029)
                     /locus_tag="AC16A0_09930"
     CDS             complement(6269..>7029)
                     /locus_tag="AC16A0_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007634171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3105696.1"
                     /translation="MQVLIPNALQPAECEEVILCRMLGKAIVLVREDQLSLAIGRRGQ
                     NVRLASQLSGWDIDIMTEAEESERRQKEFTARTELFVNALDVDETLAQLLASEGFASV
                     DEVAYVDQREIASIDGLDEQTAEELQNRAREYLDRAAQELDQRRVELGVLDELKEIEG
                     VTPQMLVALGEADIKSIEDLAGCASDDLIGWTERKDGQTTKHKGAFSALEISADEANA
                     LIMQARVRAGWIEAPAEEPAPEEVVAEAEAEAQS"
BASE COUNT         1108 a         2364 c         2172 g         1385 t
ORIGIN      
        1 tgatcgctgg gcggcagcag cgggtggatg gcgcgccagg caagcttgcc gtgtccgatc
       61 tcgcggcggc cggtcgctcc catgcggccc gtctcgccga ccgagaaggg cgggaagttg
      121 tagtgcagca ggaaggtgcc cttagtcgtg ccgtcgagcg agtcgatgta ctgctcgtcc
      181 tcgccggtgc ccagcgtggt gaccaccagc gcctgggttt cgccgcgggt gaacagcgcc
      241 gaaccatggg tacgcggcag tacgccagcc tcggcgacga tcttgcgcac cgtcttggtg
      301 tcgcggccgt cgatgcggag gcccgtatcg aggatggcgt tgcgcaccac atccttttcg
      361 agatgcttga aggcctcgcc gaccttgttg gcgggcggcg cgtcaggctt gtcctcgccg
      421 gtcagcgcgg ccagaacctt gtccttggcc ttggcgacgg cttcgtggcg ggcttccttg
      481 gtcgggatgg catagccggc gcgcacctcg gcctcggcga ttgccttgac ctggttgtag
      541 agcgcctcct cgtccggcgg cgtgaagtcg cgcgggctgc gggctgagcg ttcggccatg
      601 cggatgatgg cctcgatcac cggctggaag ccgcggtggc cgaacatgac ggcgccgagc
      661 atgatctctt ccgagagttc cttggcctcg gactcgacca tcaggaccgc gtcctgcgtg
      721 ccggcgacca cgaggtcgag ttcggagacc ttctgctgtt ccacggtggg gttcagcaca
      781 tattccccgt tgatgtagcc gacacgggcg gcaccgatcg gacccatgaa gggaatgccc
      841 gacagggtga gcgcggccga agatgcgacc agcgccagga tgtcggtgtc gttttcgaga
      901 tcgtaggaga gcacggtggc gatcacctgc gtctcgttgc ggaagccggc cacgaagagc
      961 gggcggatcg ggcggtcgat caggcgggaa atcagcgtct cgcgctccgt cggacggcct
     1021 tcgcgtttaa agtatccgcc cgggatcttg cccgcggcat aggtcttttc ctggtagttg
     1081 acggtcagcg ggaagaagtc gattcccggc ttggcgctgc gctcggcaac ggcggtcgcg
     1141 agaacggtgg tctcgcccat cgaggccagc acggccccat cagcctggcg ggccacacgg
     1201 ccggtttcaa gccgcagggt tgcgctgccc caggtgattt cttcaacatc gatattgaac
     1261 attctgtttc ctctttgccc ttcgccgccg cttcactcgc gtcaccacac ctggacggca
     1321 tcccctttgg gcaactggaa cccggcccta ttgccggatt cccgatacgg ggacgcgggc
     1381 ggcaaaccgg tgatccgccc gctcatacgc tttcgacttt tcgtcgtccg cttaacggcg
     1441 gatttcgaga cgcttgatca gcgtctggta gcggccttca tccttgcgct tgagataatc
     1501 aaggagcgaa cgacgcttgc tcaccatcgt caggagacca cggcgggaat gattgtcctt
     1561 cttgtgggtc ttgaagtggt cggtgaggta gttgatgcgt tccgtgagga tcgcgacctg
     1621 gacctcgggc gaacccgtgt cgccaggttt cgtggcaaac tctttcacga gcgcaacctt
     1681 gcgctcagca gtcatggtca ttcggtcatc tccaaatggt tacaggttaa agagacgctt
     1741 cggcttgagc gttccctgct cgacagaccc gatcccgagc ggcttgccgc cgctggtgat
     1801 cagaaccgcc tcatgagcga tgggagcatt cgcgcctctg agaaggacgg attgtccctg
     1861 tctcaggcgt tgggcctgct gctcgtcaat ggccagtgcc gggatgccgt ccagcacggt
     1921 ctcgatgggt cgcagaaccc cgtccatggc gtttccgccg ggcgccttat ggcgcaattc
     1981 ctcaagcttt tccagcggaa tcatgtgggc ctcggtgaga gctccgacag cgaggcgccg
     2041 cagcatggtg acatggccga ggcagcccag ggtccggccc atgtcgcggg cgagcgaacg
     2101 gacataggtt cccttcccgc aggtgacttc gaaggtggcg ctgtcctgct cgcagaccgc
     2161 gatcagccgc agatcatcga ttctgaccgc ccggctggcg aggaccacgt ccgccccgga
     2221 ccgggccagg tcataggccc gctcgccctc aaccttgatt gccgaaaagg ctggcgggac
     2281 ctgctggatt tcgcccttga accgggcgag tatcgattca atgctttcca gagacggccg
     2341 gttggccgag cacgtcgtaa cgacaccttc gagatcatcg gtggagcgct cctcgcccca
     2401 tttcaccgta aagcgatagg ttttgacccc ttccatggcg gcggagaccg tcttggtggc
     2461 ctctcccagg gcaatgggca gcaaacccgt cgccagggga tccaaagttc cgccatgtcc
     2521 ggccttctcg gcaccgaaga gccagcggac cttgccgacg gcctgcgtcg aggtcatgcc
     2581 caagggcttg tccaatgcca cccagccatg gaccggcgag ccgcgtttct tcttcattcc
     2641 tcggtcttga ggtcgcgggc aacatccggg cgacgaagga tttcatcgat cttggacgca
     2701 tagtccaatg ccgtatcgat ccgaaagcga atgtccggca tgaacttcat gtcgagtttc
     2761 ggcgcgataa gtccccgaat atacttccgg ttccggttca atgcctcaag ggctttgggt
     2821 tccgctccgg caatcagcgt gcggatatag gctgtggcga tcttcagatc cggcgaaatc
     2881 tgcacctcat gcacagtcac gccgagccgc tcgagctccg gatcgcccgt ctcgccgcgc
     2941 aacaggatgc ccgcaagcgc atggcggatc agctcacccg cccggagctg tcgctgggat
     3001 ggtccggcag ctggtttcat gtcgtcctca atccagcgag cgctgaacct tctcgaccct
     3061 gaaacactcg atcacgtcgc cggcgcgcat gtcctggtag ttctcgaagg ccatgccgca
     3121 ctcctgaccg acctgaactt ccttgacctc gtccttgaag cgcttgagcg tcgacagggt
     3181 accttcgtgg atcaccacgt tgtcgcggat gaggcggacc ttggcgccgc gctgcaccga
     3241 gccctcggtg acctgacagc cggccacctt gccaaccttg gtgatgttga agatttccag
     3301 gatcctagca ttgccgagga aggtctcgcg cagctccggt gcgagcttac ccgacatggc
     3361 ggccttgatg tcgtccacca agtcgtaaat gatgttgtaa tagcggatct cgatgccaga
     3421 gcgctctgcc gcgtcgcggg cctggccgga ggcgcggacg ttgaagccca gaaccaccgc
     3481 tccagaggcc tgcgccaggc cgatgtcgct ctcgttgatg ccgcccaccg catagtggat
     3541 gacgcgggcc cggatctcgt cgttgccgat cttctccagc gcctgcacga tggcttccgc
     3601 cgaaccctgc acatcggcct tgaccaggat cgggaactcc ttgatgcccc cttccttcat
     3661 ctggctcatc atctgctcaa gcgacgagcg cgccgacgcc gcaccacggc tctcgcggcg
     3721 cttgcggtcg cgatagtcgg tgatctcgcg tgcccgcgcc tcgttgggaa cgacgtcgaa
     3781 ctggtcgccc gcctcgggcg ccgaattgag gccgagcacc tcaaccggtg aagagggacc
     3841 ggcttccttc acctgctcgc ccttgtcgtt gatgagtgcg cgcacgcggc cccaggcaga
     3901 accggccacg aaaatgtctc cgaggcgcag cgtgccgcgc tggaccagca cagtcgcgac
     3961 gggtccgcga cccttgtcga gctgggcttc caccaccagg ccgcccgctt cgcggtcggg
     4021 attggccttc agatcaagca cttcggcctg cagcaggatg gtatccagca gcttgtcgag
     4081 attggtgccc ttgagcgccg acacctcgac ctccagggta tcgccgccca tgctctcgac
     4141 cacgatctcg tggcgcagca gatcggtgcg gaccttgttg ggattggccg agggcttgtc
     4201 gatcttgttg accgccacga tgatgggaac atcggccgcc cgcgcgtgat tgatggcttc
     4261 aaccgtctgg ggcatgacgc cgtcatcggc ggcaaccacg agcaccacaa tatccgtggc
     4321 cttggcgccg cgggcgcgca tggcggtgaa ggcttcatgg cccggcgtat cgatgaaggt
     4381 gatcaggccg gtcctggtct gcacctgata ggcaccgata tgctgcgtga tgccaccggc
     4441 ttcgccggac accacattgg tcttgcgaat ggcgtcgagc agcgacgtct tgccgtgatc
     4501 gacatgaccc atgatggtga ccacgggcgg acgcgactgc agcgaaccct cgtcgtcggt
     4561 atcgccggcc aggccttcga ccacgtcgga ctccgacacg cgcttgacct tgtgccccat
     4621 ttcctcggcg atgagctgcg ccaggtcggc atcgatcaca tcgttgatcg tatgcatctg
     4681 gccctgcttc atcagcaact tgatgatgtc gacggcgcgc tccgccatgc ggttggcaag
     4741 ctcctggatg gtgatcgcct cggggatcac cacctcgcgg ctcaccttct cggtcggcat
     4801 ctggaagccc tgggcctgct tcttgagacg ttcggtccgg cggcggaaag cggccaccga
     4861 acggccgcgc tcgccctctt cctcggtcag ggcattgacc accgtgaggc ggctgcggcg
     4921 cttgtcggca tcggtcttcg cgcgcgtcgg caccggcgcc ttgagttcac gtttcgccgg
     4981 gccgccggac cgcgcggtcc gcggcccatc gtcgtcatca ttgacggggc gcttggccgt
     5041 taccgtcgag ggcttcatcg gaaccgtcgc cggcgtcacc acgccggccg gcgtcttggg
     5101 cgccagcttc ttggcggcct cctcggcctt gcggcggccc tcctcctcga cccggtgacg
     5161 ggcatcgtcc tcggccttgc gccgggccgc ggcctcgcgc tcgatgctct cgcgctcatt
     5221 gagctcgctg cggcggcggg cttcctcttc ctgccgcttg cgttcatcgg cctcacgcag
     5281 gcgcgcatcc gacaaggccc ggtcgcgggc ttccttctcc tcggccgtca gcgtacgcag
     5341 gacgacgccg gaacggccag ctcctccaga tggtgcctgc gggcgctgct gcgtggctgt
     5401 ctgcgacgca ccctgcgcca ccttcggctg cgactgaaaa gccgcgggct tggattctgc
     5461 tgccgcgggc ttgtcgtcgc ccatgcgctt gcgcttggtc tccaccacca cggtcttggt
     5521 ccggccatgg gcaaaactct gcttcacccg gctctgctcg accacgggac gcttcaaggt
     5581 cagcgtgccg cctccagtgc gcgcgccatc ggccttgcgg tcattatcgt tgctgtccgt
     5641 catactctct cttcagatcc cttcgacccc aacggggcgt caaaaacgtc aatccgccgc
     5701 acggcggcaa cgagcttttc ggccagcccg ccctttgtca cggcagcatg taccacattt
     5761 gaccgtccaa atgccaaatc caattcatcg cggctgaaca ttcttaccgt catcacgccg
     5821 tcaccggcca tccggtcaag cttgcggcag ccgtccggct gggcatcggc ggcatggcag
     5881 agaatcctca cccggcccct ggccaaagcc tcctccacct tcatgaaacc ggtcaccgcc
     5941 tcccccgcct tcttggccag ggaaaaggtg gacagggcat tttgtcggag caggcgcgcc
     6001 accatgtcgg gaaggccgtc agggcttcgg gtttcctcag aaaagccctt tgaaaacaaa
     6061 tgtttcttga ccgcctctgc cacccgggac cggttcagcg tcacccagac cccccgtccg
     6121 ggcagcttgc gggccagatc gggcaccgcc acaccgtcgg gcgaacgcac gaagcgcaac
     6181 agcacggcct catccagaca ctcccgtgtc acgatgcaca tgcgtgtggc caaatcgctg
     6241 tccgcctcca tgcttcgccc tgcccgcctc agctctgggc ttccgcctcg gcttcggcaa
     6301 cgacctcttc cggcgccggt tcctcggccg gcgcctcgat ccagcccgcc ctgacacggg
     6361 cctgcatgat cagcgcattg gcctcgtccg ccgagatctc gagtgccgag aaggcgccct
     6421 tgtgcttggt ggtctgacca tccttgcgtt cggtccagcc gatcaggtca tccgatgcgc
     6481 agccggcaag gtcctcgatc gacttgatgt cggcctcgcc gagcgcgacc agcatctgtg
     6541 gcgtcacccc ctcgatctcc ttgagttcgt cgagcacgcc gagctccacg cggcgctggt
     6601 ccagctcctg tgcggcccgg tcgagatact cgcgggcacg gttctgcagc tcttccgccg
     6661 tctgctcgtc aaggccatcg atcgaggcaa tctcgcgctg gtccacatag gccacttcgt
     6721 caaccgaggc gaagccctcg gaggccagca gctgcgccag cgtctcgtcc acgtcgagcg
     6781 cattgacgaa gagctcggtc cgggcggtga attccttctg acggcgctcc gactcttcgg
     6841 cttcggtcat gatgtcgatg tcccagccgg acaactgcga ggccaggcgc acgttctgcc
     6901 cgcgccggcc gatggccagc gagagctggt cctcgcgcac gagcacgatc gccttgccca
     6961 acatgcggca aaggatcacc tcttcgcact ccgccggctg cagggcgttg ggaatcagta
     7021 cctgcatgt
//
LOCUS       DBNGVR010000467         1959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3514894, whole genome shotgun sequence.
ACCESSION   DBNGVR010000467 DBNGVR010000000
VERSION     DBNGVR010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3514894"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /gene="uvrC"
                     /locus_tag="AC16A0_09935"
     CDS             complement(<1..1871)
                     /gene="uvrC"
                     /locus_tag="AC16A0_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3105697.1"
                     /translation="MSESDQAPTGPEIIHDFVTRLPGKPGVYRMYDAKGDVIYVGKAR
                     NLRNRVSNYTRFGNHTSRIAAMIALTRHMEFVTTSTEAEALLLEANLIKKLKPRFNVV
                     LRDDKSFPYILIARDHPVAQLAKHRGARNRKGNYYGPFASAGAVNRAINTLQKAFLLR
                     SCNDSVYANRTRPCLLYQIKRCAAPCVGYVEPAAYESLVAEAEAFLGGKSQAVKAELA
                     RHMEEAADRLDFEQAARIRDRIQAMSFVTAQQGINPQTVNEADVFGLFQEGGQTSIQV
                     FFFRSGQNWGNRAYYPKADRAAEAGEVLGAFLAQFYDDKPVPGLILLSHDIQECALLE
                     EALSTHAGHKVEIAVPQRGEKRLLVEHAVTNAREALGRKMAESSSQAKLLEGVARVFR
                     LAETPRRIEVYDNSHIQGSNAVGAMVVAGPEGLMKSQYRKFNIRGEDATADDFAMMRE
                     VLTRRFTRLMKEQGDREAEVAPGEAVQWPDLVLIDGGQGQLSAAKAVLDELGLSDLPL
                     AGVAKGPDRDAGREHFYVMGQPSFMLEARDPVLYYIQRLRDEAHRFAIGSHRARRSKA
                     IGVNPLDEIAGIGPNRKKALLQSFGSAKAVARASVTDLAAVEGVSTALAQAIYDHFH"
BASE COUNT          325 a          615 c          660 g          359 t
ORIGIN      
        1 tcatggaagt ggtcgtaaat agcctgcgcg agcgccgtgc tgaccccctc cactgccgcc
       61 agatcggtga cgctggcccg ggccacggcc ttggctgaac caaagctctg cagcaatgcc
      121 ttcttgcgat tggggccgat accggcgatc tcgtcgagcg gattgacgcc gatggccttg
      181 ctgcgccgcg cccggtgcga cccgatggcg aagcgatgcg cctcgtcgcg caggcgctga
      241 atgtagtaga ggaccggatc tcgggcctcg agcatgaagg acggctggcc catgacgtag
      301 aaatgctcgc ggccggcatc gcggtccggc cccttggcca cgccggcaag cggaagatcg
      361 gagagcccca gttcgtcgag gacggccttg gccgccgaga gctggccctg gccgccatcg
      421 atgaggacca gatccggcca ttgcacagcc tcgcccggcg ccacttcggc ctcccggtca
      481 ccctgttcct tcatcaaccg ggtaaagcgg cgggtgagca cttcccgcat catggcaaag
      541 tcgtccgctg ttgcgtcttc gccgcggatg ttgaacttgc gatattgcga tttcattaac
      601 ccctcggggc ctgccacgac catggcgccc acggcattgc tgccctggat atgggaattg
      661 tcgtagactt cgatccgacg tggtgtctct gccagccgga agacccgcgc cacgccttca
      721 agcagcttcg cctgggagga ggactccgcc atcttgcggc ccagcgcctc gcgggcattg
      781 gtgacggcat gctcgaccag cagccgcttc tcgccgcgct gcggcacggc aatctcaacc
      841 ttgtggccgg catgggtgga gagtgcttcc tcaagcaagg cgcattcctg gatgtcatgc
      901 gaaagaagga tcaggccagg aaccggcttg tcgtcataga actgggccag gaaggcgccc
      961 agtacctcgc ccgcctcggc ggcgcggtcg gccttgggat aataggcgcg gttgccccag
     1021 ttctggccgg agcggaagaa gaagacctgg atcgaggtct ggccgccctc ctggaagaga
     1081 ccgaagacat ccgcctcgtt taccgtctgc gggttgatgc cctgctgggc ggtgacgaag
     1141 ctcatggcct gaatgcggtc gcggatgcgg gcggcctgct cgaaatcgag gcgatccgcc
     1201 gcctcttcca tgtgccgcgc caactcggcc ttcaccgcct ggctcttgcc gccgagaaaa
     1261 gcctcggcct cggccacaag ggactcgtag gccgcaggct cgacatagcc gacgcagggc
     1321 gccgcacagc gcttgatctg gtacagcagg cagggccgcg tgcggttggc atagacgctg
     1381 tcattgcagg agcgcagcag gaaggccttc tgcaacgtgt tgatggcgcg gttcaccgca
     1441 cccgccgagg cgaaggggcc atagtagttg cccttgcggt tgcgcgcacc gcggtgcttg
     1501 gcgagctgcg ctaccggatg gtcgcgggcg atgaggatgt agggaaagga cttgtcgtcg
     1561 cgcagcacca cgttgaagcg cggcttcagc ttcttgatga gattggcctc gagaagcagt
     1621 gcttcggctt cggtactggt ggtcacgaac tccatgtggc gggtgagcgc gatcatggcc
     1681 gcgatgcggc tggtgtggtt gccgaagcgc gtgtagttcg acacgcggtt cctcaggttg
     1741 cgtgccttgc cgacatatat gacgtcgccc ttggcgtcgt acatacggta gacgccgggc
     1801 ttgccgggca gccgggtcac gaaatcatga atgatctcgg ggccggtcgg cgcttggtcg
     1861 gactcgctca tgccgcctag ataggatgcc gggaggggcc gctgcaaccg gcctccgggc
     1921 gactgctgac atgcagcggc gccttggcgt ccggcctca
//
LOCUS       DBNGVR010000468         2035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5281903, whole genome shotgun sequence.
ACCESSION   DBNGVR010000468 DBNGVR010000000
VERSION     DBNGVR010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5281903"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="AC16A0_09940"
     CDS             <1..866
                     /locus_tag="AC16A0_09940"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF004783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR3105698.1"
                     /translation="LWDRDPGQVTRAIETIVVSLRDMAAAGLISDPAAVQDRIKPAAE
                     LAEALDGAAHVQENIAELASPKQALFTEIERLAPADAVLASSTSAIMPSIIFSTLKTR
                     ERCLVAHPMNPPHLAPIVELCGAAFTSPDAIARTRNFMAGCGMAPIEVKREVEGFILN
                     RLQLAVLNEAFRLIAGGYVSAADLDRTVTEGLALRWSFMGPIETIDLNAPEGVDDYLS
                     RYGETIRRVGKSQAAAPDWPADIGGQLEAERRKAVPRDRLAEAQAWRDRRLMALARHK
                     AEAERKISRRT"
     gene            complement(850..1653)
                     /gene="fabI"
                     /locus_tag="AC16A0_09945"
     CDS             complement(850..1653)
                     /gene="fabI"
                     /locus_tag="AC16A0_09945"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3105699.1"
                     /translation="MTESLMKGKRGLVMGVANDHSIAWGIARQLHAHGAELAFTYQGE
                     AFGKRVKPLADSLGSKLLLPCDVEDIATVDGVFAELQKAWGRIDFLVHAIAFSDKNEL
                     KGLYADTTRENFVRTMVISCFSFTEITRRAAALMPDGGSLVTLTYGGSVRVMPNYNVM
                     GVAKAALEASVRYLAADYGPRGIRVNAISAGPMRTLAGAGIGDARAMFNYQQSHAPLR
                     RTITLDEVGGAGLYLLSPLSGGVTGEVHYVDSGYNIISMPRPEDLKSGD"
     gene            complement(1659..>2035)
                     /locus_tag="AC16A0_09950"
     CDS             complement(1659..>2035)
                     /locus_tag="AC16A0_09950"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657783.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HIR3105700.1"
                     /translation="ALADVGEKIDYINPHSTSTPIGDLKEIEAIREVFGSDIPPISST
                     KSLTGHSLGATGVQESIYSILMMKNGFLCESAHIEELDPEFADVPILRARKDNAKVDV
                     VMSNSFGFGGTNATLVFQRYNG"
BASE COUNT          361 a          664 c          670 g          340 t
ORIGIN      
        1 cgctgtggga ccgtgatccc ggacaggtga cgcgggccat cgagaccatc gtcgtctcgc
       61 tgcgcgacat ggccgcagcc ggcctgatct ccgatccagc cgctgtccag gatcgcatca
      121 agccggcggc cgaactggcc gaggcgctgg acggcgccgc tcacgtgcag gaaaacatcg
      181 ccgaactggc ctcgccgaag caggctctct tcaccgagat cgagaggctg gcaccggcgg
      241 acgcggtgct ggcgagttcc acctcggcca tcatgccgtc aattatcttc agtacactga
      301 agacccgcga gcgctgcctg gtggcccacc cgatgaaccc gccgcatctt gccccgatcg
      361 tcgagctgtg cggggccgcc ttcacgtcgc cggacgccat cgcgcgcaca cggaacttca
      421 tggccggctg cggcatggcg cccattgagg tgaagcgcga ggtcgagggc ttcatcctca
      481 accgcctgca gctggccgtg ctcaacgagg ccttccggct gatcgccggc ggctatgtgt
      541 ccgctgccga cctcgaccgt acggtcaccg aaggactggc gctgcgctgg agcttcatgg
      601 ggccgatcga gaccatcgat ctcaatgcgc ccgaaggtgt cgacgactat ctctcgcgct
      661 atggcgagac catccgccgc gtcggcaagt cccaggcggc cgcgcccgac tggccggccg
      721 acatcggggg gcagctcgag gccgaacggc gcaaggccgt gccgcgcgac aggctggcag
      781 aagcccaggc ctggcgcgac cgccgtctca tggcgctcgc ccggcacaag gccgaggccg
      841 agcgcaagat cagtcgccgg acttgagatc ctctgggcgc ggcatcgaga tgatgttgta
      901 acccgagtcg acgtagtgga cctcgcccgt cacgccgccc gacaatggcg agagaaggta
      961 gagcccggca ccacccacct cgtccagcgt gatggtgcgg cggagcggcg catggctctg
     1021 ctggtagttg aacatggcac gggcatcgcc gatgccggcg cccgccagcg tccgcatcgg
     1081 gcctgccgag atggcattca cccttatgcc gcgcggaccg tagtcggctg cgagatagcg
     1141 gacagaggct tccagcgccg ccttcgccac gcccatgaca ttgtagttcg gcatgacccg
     1201 caccgagccg ccataggtca gcgtcaccag cgatccgcca tcgggcatca gcgccgcggc
     1261 gcggcgggtg atctcggtaa aggagaagca cgagatcacc atggtgcgga cgaagttctc
     1321 gcgcgtggta tcggcgtaaa ggcctttcag ctcgttcttg tcggaaaagg cgatggcatg
     1381 cacgaggaaa tcgatgcggc cccaggcctt ctgaagttcg gcgaagacgc cgtcgacggt
     1441 tgcgatgtcc tcgacatcgc agggcagcag cagcttcgat ccgagcgaat cggccagcgg
     1501 cttgacgcgc ttgccgaagg cctcgccctg ataggtgaag gccagttcgg caccatgcgc
     1561 atggagctgc cgcgcgatgc cccaggcaat cgagtgatcg ttcgcgacgc ccatcaccag
     1621 gccgcgtttg cccttcatca gagattcggt catgcgggtc agccgttgta gcgctggaag
     1681 acgagggttg cgttggtacc gccgaagccg aagctgttcg acatcaccac atcgaccttg
     1741 gcgttatcct tccgtgcccg gaggatcggc acgtcggcaa actccggatc gagttcctcg
     1801 atatgcgcac tttcgcagag gaagccgttc ttcatcatca ggatggaata gatcgactcc
     1861 tgcacgccgg tggcgccgag cgaatggccg gtgagcgact tggtggacga gatcggcgga
     1921 atgtcgctgc cgaagacctc gcggatcgcc tcgatctcct tgaggtcgcc gatcggcgtc
     1981 gaggtggagt ggggattgat gtagtcgatc ttctcgccga catcggcgag cgcca
//
LOCUS       DBNGVR010000469         3221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3908956, whole genome shotgun sequence.
ACCESSION   DBNGVR010000469 DBNGVR010000000
VERSION     DBNGVR010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3908956"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /gene="tatC"
                     /locus_tag="AC16A0_09955"
     CDS             complement(<1..353)
                     /gene="tatC"
                     /locus_tag="AC16A0_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0009977 - proton motive force dependent
                     protein transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3105701.1"
                     /translation="MTQNEIDASEAPLIEHLIELRKRLMFCVIGFVLAFIVSFIFSSD
                     ILYYLVLPFKWGTGTDVNLISIKLLGVFLVKLKLAFFGGIFIAFPLIATQIYRFMAPG
                     LYSHEKNAFRPYLIAT"
     gene            complement(361..702)
                     /gene="tatB"
                     /locus_tag="AC16A0_09960"
     CDS             complement(361..702)
                     /gene="tatB"
                     /locus_tag="AC16A0_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR3105702.1"
                     /translation="MFDFGIGYTELFVIGVVAIIVIGPKDLPRVLRAVGQTVAKMRGM
                     AREFQGHLDSAMKDAGLDDVKKEIQGLKDIGTIQPVSKKPPVNDTKKAEEDFAKFFGE
                     PEKPAVSGPSA"
     gene            complement(732..953)
                     /locus_tag="AC16A0_09965"
     CDS             complement(732..953)
                     /locus_tag="AC16A0_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR3105703.1"
                     /translation="MGSLSIWHWALVILVIVLLFGRGKISDLMGDVAKGIKSFKKGMA
                     EDEPATASADPKSIEQQAAAKVEAEKTKS"
     gene            complement(1056..2186)
                     /locus_tag="AC16A0_09970"
     CDS             complement(1056..2186)
                     /locus_tag="AC16A0_09970"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105704.1"
                     /translation="MGIEAEQKPVQASDRWGRRGTAGATFAGLLTFEAVGKSFGGIAA
                     VSDVSFELKPGEIACLLGPSGCGKTTLLRIAAGIERPSRGRVLFDGSEMAGPNRFVPP
                     EKRNIGLMFQDFALFPHLSIIENVAFGLRSLPRQEANRVALHALERVGLAAYAESYPH
                     HLSGGEQQRVALARAVVPRPQVMLMDEPFSGLDQRLRDSVRSETLALLRETRASSLMV
                     THDPVEAMGLADKIFLMRQGRLIQSGTPEELYHRPADAAAARFFSDVNEFTARVRNGR
                     AETPLGSFAVPAGYAGAQALVMVRPQGIRVSKAETGIEGYVLETRFQGDDVRCAILFK
                     GIEEPVAARIAAQQAPARGLTACFEVDSGHVLVFESNGTDIT"
     gene            complement(2241..>3221)
                     /locus_tag="AC16A0_09975"
     CDS             complement(2241..>3221)
                     /locus_tag="AC16A0_09975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3105705.1"
                     /translation="TRTGGAEAMGLINANLPSLDTKPGNIMVVDNRAITADRVRGALD
                     GRHDVTIVTDGQQAIELCAQNDPPFELVVISLDMEGVDALRVCSQLKSLETTRQTPIL
                     LIVDPDDHQRLLRALDMGVNDYLIRPVDHQELQARVNTQIRRFRYTEQLRKSVQASME
                     MAVTDSLTGLFNRRYLETHLAQLVGHAINRGKPLSVITLDVDYFKTVNDTHGHQAGDR
                     VLQELAGRIRGAIRGVDLACRTGGEEFVVVLPGTEIGIAQRVAERLRQAVANRPFTAA
                     PGFALNITISAGVAALNGVDDNVDTILKQADQALYRAKREGRNCVILAAA"
BASE COUNT          594 a         1023 c         1041 g          563 t
ORIGIN      
        1 gtggcgatca gataggggcg gaaggcattc ttctcatgcg aatagagccc cggcgccatg
       61 aagcgataga tctgcgtggc gatcagcgga aaggcgatga agatgccgcc gaagaaggcg
      121 agcttcagct tcacgagaaa aacgcccagc agcttgatcg agatcaggtt gacatcggtg
      181 cccgtgcccc atttgaaggg cagcacgagg taatagagaa tgtcagacga gaagatgaaa
      241 ctgacgatga aggcaagaac aaacccgatg acgcagaaca tcagccgctt gcgcagctcg
      301 atcagatgtt cgatgagcgg cgcctcggac gcatcgatct cgttctgggt catgcgccgc
      361 ctatgccgag ggacctgaga cggcaggctt ttccggttcg ccgaagaact tggcgaaatc
      421 ttcctcggcc ttcttggtat cgttcaccgg cggcttcttg ctgaccggct ggatggtccc
      481 gatatcctta agtccctgga tttccttctt cacgtcatcc aggccggcgt ccttcatggc
      541 gctgtccagg tggccctgga actcgcgggc catgccgcgc atcttcgcca ccgtctgccc
      601 caccgcgcgc agcacgcgcg gcaaatcctt ggggccgatg acgatgatcg ccaccacgcc
      661 gatcacgaag agttcggtgt agccaatgcc gaagtcgaac atgggtaaac tggccctccg
      721 ggccgcgagc ctcagctctt cgtcttctcg gcttcaacct tggcagcagc ctgctgttcg
      781 atggacttcg ggtcagccga agcggtggcc ggctcatctt cagccatgcc cttcttgaag
      841 ctcttgatgc ccttggcgac gtcgcccatc agatccgaaa tcttgccccg gccaaacagg
      901 agcacgatca ccagaatgac cagcgcccaa tgccagatgg aaagcgaacc cattgtaaaa
      961 actcctcttt ctcgatgaga gatgcttgac ctctcgacat ggccgggcac tattgcagaa
     1021 agcccgcagc gccgcaacca aagctgcgac gtctatcagg taatgtcggt accgttcgat
     1081 tcaaagacca gaacatgccc ggaatcgact tcgaagcatg cggtaagacc gcgcgccggc
     1141 gcctgctgcg cggcgatgcg ggcggcgacc ggttcctcga ttcccttgaa caggatggcg
     1201 cagcggacat catcgccctg gaaccgggtt tccagcacgt aaccttcgat cccggtttcg
     1261 gcctttgaca cccgtatgcc ctgggggcgc accatcacca gcgcctgcgc gccggcatag
     1321 cccgccggaa cggcgaagct gcccagcggg gtttccgccc ggccgttccg cacccgcgcg
     1381 gtgaattcgt tgacgtcgga aaagaagcgg gcggcggccg cgtcggcggg ccggtggtag
     1441 agttcttccg gcgtgcccga ctggatcagg cggccctggc gcatcaggaa gatcttgtcg
     1501 gcaaggccca tggcctccac cggatcgtgg gtcaccatca ggctcgaggc gcgcgtttcg
     1561 cgcaacaggg cgagcgtttc gctgcgcacg gagtcgcgca ggcgctggtc gagccccgaa
     1621 aacggctcgt ccatcagcat gacctgcggc cgcggcacca cggcacgcgc cagtgccacg
     1681 cgctgctgct cgccccccga gaggtgatgc ggataggact cggcataggc cgcgagcccg
     1741 acacgctcga gcgcatggag ggcgacgcgg ttggcctcct ggcgcggcag gctgcgcagc
     1801 ccgaaggcca cattctcgat gatcgacaga tgcgggaaca gcgcgaagtc ctggaacatc
     1861 agcccgatgt tgcgcttctc gggcggcacg aaccggttgg ggcctgccat ttcggagccg
     1921 tcgaagagca cgcggccgcg gctcggccgc tcgatcccgg cggcgatgcg cagcaaggtg
     1981 gtcttgccgc agcccgaagg tccaagcagg caggcgattt cgccgggttt gagctcgaag
     2041 ctcacgtcgc tgaccgccgc gatgccgccg aagctcttgc cgacggcttc aaacgtcaga
     2101 agtccggcaa aggtcgcacc tgccgttccg cggcgccccc accggtctga cgcctgcacc
     2161 ggtttctgtt cagcctcgat gcccatgcgg cagccaccta caccattacg ggccatgatg
     2221 gaaaggactg atggcggcga tcaggccgcc gcgaggatca cgcagttgcg gccctcgcgc
     2281 ttcgcgcgat agagggcctg atcggcctgc ttgagaatcg tatcgacatt gtcgtcgacg
     2341 ccgttcagcg ccgcgacgcc ggccgagatg gtgatgttca gggcaaagcc cggcgcagcc
     2401 gtgaaaggcc ggttggcaac cgcctgacgg agccgttcgg ccacacgctg cgcgatgccg
     2461 atctcggtgc cgggaagcac cacgacaaac tcctcgcctc cggtccggca ggccagatcg
     2521 accccgcgaa tggcaccgcg gatgcgcccg gccaattcct gaaggacgcg atcgccggcc
     2581 tgatggccat gggtgtcgtt gaccgtcttg aagtagtcga cgtccagcgt gatgaccgac
     2641 aagggcttgc cgcggttgat ggcatgaccg acaagctggg ccaggtgggt ctccagatag
     2701 cggcggttga acaggcccgt cagcgaatcg gtgaccgcca tctccatcga ggcctgcacg
     2761 ctcttgcgaa gctgctcggt atagcggaag cggcggatct gggtattgac gcgcgcctgc
     2821 agctcctgat ggtcgacagg ccggatcagg tagtcgttga cgcccatgtc gagcgcacgc
     2881 aggaggcgct gatggtcgtc agggtcgacg atcagcagga tcggcgtctg gcgggtcgtc
     2941 tcgagcgact tcagctggga gcagacgcgc agcgcatcga ccccttccat gtcgaggctg
     3001 atcaccacca gttcgaaggg cggatcgttc tgggcacaga gctcgatcgc ctgctgcccg
     3061 tcggtgacga tcgtcacgtc gtgacggccg tcgagcgcgc cgcggacgcg gtcggccgtg
     3121 atcgcgcggt tgtcgacgac catgatgttg ccgggcttgg tgtcgagcga gggaagattg
     3181 gcgttgatga ggcccatggc ctctgccccg ccggtccggg t
//
LOCUS       DBNGVR010000470         1791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4299857, whole genome shotgun sequence.
ACCESSION   DBNGVR010000470 DBNGVR010000000
VERSION     DBNGVR010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4299857"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="AC16A0_09980"
     CDS             complement(<1..208)
                     /locus_tag="AC16A0_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR3105706.1"
                     /translation="MQILRRKSWEVPESRVTPEYVFLNRRSFMAAAAGAGVIAAAGPA
                     RAEEDPSASLYPAKVNPAFADAGRP"
     gene            337..1716
                     /locus_tag="AC16A0_09985"
     CDS             337..1716
                     /locus_tag="AC16A0_09985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3105707.1"
                     /translation="MPRAQLSAVTLPEFGTPTVEPQLTRDIYRARIARLESRMAEAGL
                     DAIVIYGDREHLANISWASGYDPRFEEALLIVVPGRRPCLLAGNEGYPYAETAAGDFD
                     RVLWQPLSLMGQPRDKCLPLRNVLRDAGLGHDLRIGLAGWKGFETEDGAFDPHWFETP
                     HYLVETLKHFGEVTNAALLFMHPVTGLRAHNEVAQLAAFEYAATKTSTAVRDFILNVK
                     PGMCERDAVRAMALDGFMQSIHINMCSGPRAKYGLPSPSLRIIERGDPIVVGHGLMGA
                     LNCRAGWMVASADELQPAIRDYIDKLVAPYFSAAIAWYETLGIGVTGGEMFDAAMRHV
                     GDPFFGIGLNPGHLIHLDEWLHSPIRKDSSMKLASGMALQCDIIPATGTDYFTSNIED
                     GVALADAETRAALQRDFPETWSRIARRRTFMTDVLGIRLKPEVLPFSSIPAWLPPFWL
                     DPGKAMAMR"
BASE COUNT          307 a          609 c          550 g          325 t
ORIGIN      
        1 ccggccgtcc ggcatcggca aaagcgggat tgacctttgc gggatagagc gaagccgacg
       61 gatcctcctc cgcccgtgcc gggcccgcgg cagcgatgac accggctccg gctgccgccg
      121 ccatgaagct gcggcgattg agaaagacat attccggcgt gacccgggat tccgggactt
      181 cccaactctt gcggcgcaga atctgcatgg gcgtgctcct tgttgggcag taggctagtc
      241 ctctacccta cgcagcacaa atcaaggccg atcacggctt ggcgaggggt ttaccccgcg
      301 gcgtgatcgg ctagtctcgg acgaaaggag cttcccatgc cccgcgccca actgagtgcc
      361 gtcacgctgc ccgaattcgg aactccgaca gtggaaccgc agctgacgcg agacatctac
      421 cgtgcccgca ttgcccgcct cgaatcccgc atggcggagg ccggactgga tgccattgtg
      481 atctacggcg accgcgagca tctggccaac atctcctggg cctcgggtta tgatccgcgc
      541 ttcgaggaag cgctgctcat tgtcgttcct ggacgccggc cctgcctgct ggccggcaac
      601 gagggctatc cctatgccga gacggccgcc ggcgatttcg accgcgtgct gtggcagccc
      661 ttgagcctga tgggccagcc gcgcgacaaa tgtttacccc tccgcaatgt cctgcgcgat
      721 gcgggcctcg gacatgacct gcggatcggc cttgcgggct ggaagggctt tgagacggaa
      781 gatggcgcct tcgatccgca ctggttcgag acaccgcact atctcgtcga aacgctgaag
      841 catttcggcg aggtgaccaa tgcggccctg ctcttcatgc atcccgtgac tggcctgcgc
      901 gcccacaacg aggtggcgca gcttgctgcc ttcgaatatg ccgccaccaa gacgtcgacg
      961 gcggtccgcg acttcattct gaacgtgaag ccgggcatgt gcgagcgcga cgcggtgcgc
     1021 gccatggctc tcgacggctt catgcagtcg atccacatca acatgtgttc aggcccccgg
     1081 gcgaagtatg gcctgcccag tccaagcctt cggatcatcg aacgcggcga cccgatcgtg
     1141 gtgggtcacg gcctcatggg tgcgctcaac tgccgggccg gctggatggt cgcatcggcg
     1201 gatgaactgc aacccgcgat ccgcgactac atcgacaagc tggtcgcgcc atacttctct
     1261 gctgccatcg cctggtacga gacgctgggc atcggcgtga cgggcggcga aatgtttgac
     1321 gccgccatgc gccacgtggg cgatcccttc ttcggcatcg gcctcaatcc cggccacctc
     1381 atccatctcg acgaatggct gcattcgccg atccgcaagg acagcagcat gaagctcgcc
     1441 tccgggatgg cgctgcaatg cgacatcatt cccgcgacgg gcacggacta cttcaccagc
     1501 aacatcgagg acggcgtggc actggccgac gcggaaaccc gtgccgccct gcaacgcgac
     1561 tttcccgaga catggtcgcg catcgcgagg cgccgcacct tcatgacgga tgttctcggc
     1621 atcaggctga agccggaagt cctgcccttc agcagcattc cggcctggct gccgcccttc
     1681 tggctcgatc ccggcaaggc gatggcgatg cgatgattgg cccgctgtcc gctcaggccg
     1741 cctccgcctt gcgcgagttc accacctggt ccgccgctcg ccccgtcagt t
//
LOCUS       DBNGVR010000471         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4300063, whole genome shotgun sequence.
ACCESSION   DBNGVR010000471 DBNGVR010000000
VERSION     DBNGVR010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4300063"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC16A0_09990"
     CDS             complement(<1..525)
                     /locus_tag="AC16A0_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105708.1"
                     /translation="MDKRAPLIPGHEAILDRLVATALSAGSIASQTDKYFTNTSRIVA
                     AHGDSQVTFAVFMRRRVVAALEPTIRLIRHFVPDVRIRRFYEEGDVVPSESKMLEITG
                     SMARLSEVETLLLQKTGFPCVSANNAYEMCRALPNAAFMDMHARHASGAEMNILAAYG
                     AAVGSAAARRADSAV"
     gene            complement(560..1336)
                     /locus_tag="AC16A0_09995"
     CDS             complement(560..1336)
                     /locus_tag="AC16A0_09995"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3105709.1"
                     /translation="MGAAHFDLRARLATGEPFLFSWLSIPSAGLAAQVARLPFDGACL
                     DMQHGMIGFSDAVPMVAAINAVGKPAIIRALWNDPAIPGQALDAGAVAVISPMINSRA
                     EAEALVKAAKYPPIGGRSWGGYTALQASGLTPGEYLREANRMTMVFAMIETVQALDAV
                     AEIAAIPGLDGLFVGPSDLSIALSRGAGIDKTGAQTLAAMEKVVAAARANNLVAGAFG
                     GGAEVIKAYLGLGFTFIAAAVDVDLLQTGAAALMKSLKGV"
     gene            complement(1337..1660)
                     /locus_tag="AC16A0_10000"
     CDS             complement(1337..1660)
                     /locus_tag="AC16A0_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HIR3105710.1"
                     /translation="MTTFDKREEGFEKKFAHDEELRFKAGARRNKLLGLWAAEKLGLS
                     GAEAEAYAKTVVAADFEEAGDEDVFRKVRKDFDAKGVDQSDHQIRRTMDEMMGQAIEQ
                     IKAAG"
BASE COUNT          344 a          635 c          635 g          331 t
ORIGIN      
        1 cactgccgag tctgcccggc gcgcggcggc actcccgacg gccgcgccat aggcggccag
       61 gatgttcatt tcggccccgc tggcgtgccg cgcatgcatg tccatgaagg ctgcgttggg
      121 cagcgcccgg cacatctcat aggcgttgtt ggcggatacg caggggaacc ccgtcttctg
      181 cagcagcagg gtttccacct ccgagagccg cgccatggac ccggtgattt ccagcatctt
      241 gctttcggag ggcacgacat cgccctcctc gtagaagcgc cggatgcgca cgtccgggac
      301 aaagtgccgg atcagccgga tggtgggttc cagtgcggcc acgacgcggc ggcgcatgaa
      361 gacggcgaag gtgacctggg aatcgccatg cgcagccacg atgcggctgg tgttggtgaa
      421 atacttgtcg gtctgcgagg cgatgctgcc cgcgctcagc gccgtggcga cgagccggtc
      481 gagaatggcc tcatggccgg gaatgagcgg cgcgcgcttg tccatgatcg aggcagtctc
      541 gcgcgaaatc cgggggaggt caaacgccct tgagcgactt catcaatgcg gcggccccgg
      601 tctgcaggag atcgacgtcc acggcggccg cgatgaaggt gaagcccaga cccagatagg
      661 ccttgatcac ctccgccccg ccgccgaagg ccccggccac caggttgttg gcccgcgcgg
      721 ccgcgaccac cttctccatg gcggccagcg tctgcgcgcc ggtcttgtcg atgccggcgc
      781 cgcgcgagag ggcgatcgac aggtcgctgg ggcccacgaa cagcccgtcg aggccaggga
      841 tcgcggcgat ctcggcgacg gcatccaggg cctgcacggt ttcgatcatg gcaaagacca
      901 tggtcatgcg gttggcctcc cgcagatatt cgccaggcgt caggcccgaa gcctggaggg
      961 ccgtgtagcc gccccagctg cgcccgccga taggcggata cttggccgcc ttcaccagcg
     1021 cctcggcctc cgcccggctg ttgatcatcg gggaaatcac ggcaacagca ccggcatcca
     1081 gcgcctggcc cggaatggcc ggatcgttcc acagcgcccg gatgatggcg ggcttgccca
     1141 ccgcgttgat ggcggcaacc atcggcacgg catcggagaa gccgatcatg ccatgctgca
     1201 tgtcgaggca ggcgccgtcg aagggcaggc gcgccacctg tgccgccagg ccggcactgg
     1261 gaatcgacag ccaggagaac agaaacggct cgccagtggc gagccgcgct ctcagatcga
     1321 aatgggcggc acccacttac ccggctgcct tgatctgctc gatggcctgt cccatcatct
     1381 cgtccatggt gcggcggatc tgatgatccg actgatcgac acccttggca tcgaagtcct
     1441 tgcgaacctt gcggaaaacg tcttcgtcgc cggcctcctc gaagtcggcc gccacgaccg
     1501 tcttggcata ggcctcggcc tcggccccgg agagacccag cttctcggcc gcccagagcc
     1561 ccaagagctt gttccggcgg gcacccgcct tgaacctgag ttcctcgtcg tgggcgaact
     1621 tcttctcgaa gccttcctcg cgcttgtcga aggtggtcat gcagcactcc tgttaagcca
     1681 ttggcagctt gaaatagcca gtgacccccg ccaaatcaac ctcggaaggg ccattgtgcg
     1741 ccgcacaacg atggtttatt gagcggcgat actggactgg ggattaggat gccccttttc
     1801 cgcgacagtt atggcgtcag gtcccgctcc ggactagggt gtgcggaggt agataagggt
     1861 attgatgagg atacttgaga tgaacagcag gcgtacccgc atctacgagg gaaaggccaa
     1921 gatcctctac gagggtccgg aaccc
//
LOCUS       DBNGVR010000472         2845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5480729, whole genome shotgun sequence.
ACCESSION   DBNGVR010000472 DBNGVR010000000
VERSION     DBNGVR010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5480729"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="AC16A0_10005"
     CDS             complement(<1..631)
                     /locus_tag="AC16A0_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3105711.1"
                     /translation="MTRRQILLALLPPLFFGTGFTIAKPAVAHFPPLFMMLMVYGGIA
                     VVLAITHRERLKTPVGSIIAISSLAVTIQGALLFWGLREMSATAANLILQIQIPIAVL
                     LDWAIMKEKLDLKKSIGTALAIVGVAIVIGLPEEAPAVIPTLMIIAGAFTWALGQVLA
                     RKLGRDSGVGLLKANAFGSVPQLAIATILLEQGQWQSVQSAGMLEWSMLA"
     gene            757..1845
                     /locus_tag="AC16A0_10010"
     CDS             757..1845
                     /locus_tag="AC16A0_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR3105712.1"
                     /translation="MLGAGRLTALAGQARGRGAAINMSGRFERLSRAIYDDGWNSLEE
                     LPPFRTEVFEESPKTIIARNESPDISFDRSVNPYRGCEHGCSYCYARPTHAYMGLSPG
                     LDFESRIFAKPNAAELLRAELSAPGYVPRTLAIGTNTDPYQPVERSRRITRQLLEVLS
                     DFNHPVGIVTKSALVLRDIDILKSMAERGLVKVALSVTTLDPKLSRSMEPRAATPPKR
                     LAALEMLSAAGIPTVVMVAPIIPAVNDAEIEAILKAAAAAGAREAGYVMLRLPLEVRD
                     VFTDWLDKTMPERAAKVMSLVKSIRQGKANDSTFGRRMTGTGPYAWTIGRRFELACQR
                     LGLNASRVRLATDLFRRPPQPGEQLTLL"
     gene            1867..2484
                     /locus_tag="AC16A0_10015"
     CDS             1867..2484
                     /locus_tag="AC16A0_10015"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3105713.1"
                     /translation="MSRSGPHFRFEQDAVSRGLAPVCGIDEAGRGPWAGPVVAAAVIL
                     DPARIPEGLDDSKKLSAAQRDALFDPILASAQVGIGIADVARIDRDNILQATFWAMAE
                     ALRKLATPPSLALVDGNRAPQLPCPVRTVIAGDAESLSIAAASIIAKVTRDRLMMELD
                     RTFPQYGFARHKGYGTALHQACLDRHGPCPQHRRSFAPVAARLAR"
     gene            2574..>2845
                     /locus_tag="AC16A0_10020"
     CDS             2574..>2845
                     /locus_tag="AC16A0_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR3105714.1"
                     /translation="MGYQTRPDIRPLHNRILVGDCVSELKKIPAGSVDLVFADPPYNL
                     QLAGSLTRPDQSKVDAVDDAWDKFASFEHYDRFTKAWLSECRRVLKP"
BASE COUNT          487 a          984 c          897 g          477 t
ORIGIN      
        1 aggcgagcat cgaccattcg agcattcccg ccgactggac ggactgccat tgcccctgct
       61 ccagcaggat cgtggcaatc gccagctgcg gcacgctgcc gaaggcgttg gccttcagaa
      121 gcccgacgcc actgtcgcgg ccgagcttgc gtgccagcac ctggccaagc gcccatgtga
      181 aggcgccggc aatgatcatc agtgtgggga tgactgcggg agcctcctcc ggcaggccga
      241 tgacgattgc cacacccaca atcgccagtg ccgtgccgat cgacttcttg aggtcgagct
      301 tctccttcat gatcgcccag tcgagaagga cggcgatcgg gatctggatc tggaggatga
      361 ggttggcggc cgtggccgac atttcgcgaa gcccccagaa cagcagcgcg ccctgaatgg
      421 tgacggccaa ggacgagatc gcgatgatcg agccaaccgg cgttttcagc ctttcgcgat
      481 gggtgatggc gagcacgact gcaattccgc catagaccat cagcatcatg aagagcggcg
      541 ggaaatgcgc cacggccggc ttggcgatgg tgaagccggt accgaagaac agcggcggca
      601 ggagcgcgag gagaatctgg cggcgggtca tgcagcttgc tgaacgaagt tgggacttcg
      661 aacaagtttg ttcttgattt gttccgaccg gctggctaag ttttgcgcat gcccacgctg
      721 atcgacaggc gccagtccac cggcctcgac cccgccatgc tgggggcggg ccgcctgacc
      781 gcgctcgcgg gacaggcccg tggccggggc gccgccatca acatgagcgg ccgcttcgaa
      841 cgcttgtccc gcgccatcta tgatgacggc tggaacagcc tcgaagagct gccgcccttc
      901 aggaccgagg tcttcgagga gagcccgaag accatcatcg cgcgcaacga gtcgcccgac
      961 atttccttcg accgctcggt caatccctat cgcggttgcg agcatggctg ctcctactgc
     1021 tatgccaggc caacccatgc ctatatgggc ctgtcgccgg ggctcgattt cgagagccgg
     1081 attttcgcca agcccaatgc ggccgagctg ctgcgcgccg agctctcggc gcccggctac
     1141 gtgccgcgca ccctggccat cggcaccaac accgatccct accagccggt cgagcgcagc
     1201 cgccgcatca cccgccagct gctcgaggtg ctgagcgact tcaaccaccc ggtcggcatc
     1261 gtcaccaagt cggcgctggt gctgcgcgac atcgacatcc tcaagtcgat ggccgaacgc
     1321 gggctggtga aggtggcgct gtcggtcacc accctcgacc cgaagctgtc ccgcagcatg
     1381 gagccgcgcg ccgcaacgcc gccgaaacgc ctggcggctc tggagatgct ctcggccgcg
     1441 ggcattccca ccgtggtcat ggttgcgccc atcattccgg cggtgaacga cgccgaaatc
     1501 gaggccatcc tcaaggccgc agcggctgcc ggcgcgcgcg aggcgggcta tgtcatgctg
     1561 cgcctgccgc ttgaggtgcg cgatgtcttc accgactggc tggacaagac catgcccgag
     1621 cgggcagcca aggtcatgtc gctggtgaaa tcgatccgcc agggcaaggc caatgattcc
     1681 accttcggcc gccgcatgac cggcaccggc ccctatgcct ggaccatcgg gcggcgcttc
     1741 gagctggcct gccagcgcct cgggctgaac gccagccggg tcaggctggc aaccgatctt
     1801 ttcaggcggc cgccccagcc gggtgagcag ttgacgctgc tctgaggcct cgccacacag
     1861 ggacgcatgt cccggtcagg cccccatttc cgattcgagc aggacgcggt gtcgcggggt
     1921 ctggcgcctg tctgcggcat cgatgaagcc ggccgtggtc cctgggccgg cccggtcgtc
     1981 gccgctgccg tgatcctcga tccggcacgc atccccgaag gcctcgacga ttccaagaag
     2041 ctcagcgccg cgcagcgcga cgcgctcttc gatccaatcc tggccagcgc gcaggtcggc
     2101 atcggcatcg ccgatgtcgc gcgcatcgac cgcgacaata tcctgcaggc cactttctgg
     2161 gccatggcgg aagcgctccg taagctggcg acgccgccaa gccttgccct tgtcgacggc
     2221 aaccgcgcac cgcagctccc ctgtccggtc cggaccgtca tcgccggcga tgccgaatcg
     2281 ctctccatcg ccgccgcctc catcatcgcc aaggtcaccc gcgaccggct gatgatggag
     2341 ctcgaccgca ccttcccgca atacggcttc gcccgccaca agggctatgg aaccgcgctg
     2401 catcaggcct gtctcgaccg tcacggcccc tgcccgcagc accgccggag cttcgcgccg
     2461 gtggccgccc ggctggcgcg ttaaccactt ccccggaatt cgattcacgc cgagtccgcc
     2521 aggattcatg gtggcgattc agagtcattt gcgttgcgtt tcgagggtgt gggatgggtt
     2581 accagacacg gccggacatc cggccgctgc acaacaggat cctggtgggg gactgcgttt
     2641 cggaactgaa gaagattccg gcgggcagcg tcgatctcgt cttcgccgat ccgccctaca
     2701 atctccagct cgcgggcagc ctcacgcgtc ccgaccagag caaggtcgac gccgtcgacg
     2761 atgcctggga caagttcgcc agcttcgagc attatgaccg cttcaccaag gcgtggctgt
     2821 cggaatgccg ccgcgtgctg aagcc
//
LOCUS       DBNGVR010000473         2674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_569862, whole genome shotgun sequence.
ACCESSION   DBNGVR010000473 DBNGVR010000000
VERSION     DBNGVR010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_569862"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="AC16A0_10025"
     CDS             <1..1098
                     /locus_tag="AC16A0_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3105715.1"
                     /translation="IPVIEMVEIGAGGGSIARVDRLGRITVGPDSAGSTPGPVCYGRG
                     GTEPAVTDANLALGKVDPAYFAGGKIALDEKSARQALQKNVGDKLALKDFWPAAGVTE
                     IVEENMAYAARVHAIELGRDIAACTMIAFGGGAPLHACRLAEKVGVTTVIVPKGAGVG
                     SAIGFLRAPVAYEITRSAVTSLESFDARRVNRLLDSMTRDARAVVEPALAGAKASVQI
                     IADCRYVGQGHEIRVSVPVRKLSDADGKRLKAAFEKTYEQVYGLRIPNQEVEAITWSV
                     TVSSKTEKPRRAARAARRAAPQPRSRRTIYDPALGKQVNAPVYWRFDMKPGASIKGPA
                     IIAEDETSTIVGARFGATINSLGYIVMERRS"
     gene            1095..>2674
                     /locus_tag="AC16A0_10030"
     CDS             1095..>2674
                     /locus_tag="AC16A0_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3105716.1"
                     /translation="MSKSALDAIRTQVMWNRLIAVVEEQAQSLLRTAFGTITREAGDL
                     SAGVYDTSGNMIAQAMTGTPGHVNTMATAVAHFFKHFPQTTMKPGDVYVTNDPWLGTG
                     HLFDYVVMTPVFLRRKLVAFFASTCHVIDVGGVGMSAKANSSFEEGTLIPHMKMRKEG
                     VLNEELIGIILANSRNPVEVRGDLLSLISANDTGARRLLSMMEEFNLSSIEPLARHIL
                     TQSEKGAREAIKALPDGEWTYDMPLDGYERPILIKSKLTIRRGKITIDFSGSSPASIH
                     GINSPRTYTHAYSVFGLKAVIAPHVPNNIGSLSCFDLVTEPGTCVDPTRPSPVTARHV
                     IGQMLADCTFGCLAQALPGKVQAESAGSIWILGLNSAHGRVPAEETAKARYFGVINIG
                     LGGIGGRPGKDGLSTMAFPSGVGTIPVEITETQCPLYFKRKEYLPDSGGAGEFRGGLS
                     QTIEIANREAAPFTISAATFDRIHNPAQGRDGGRPGRKGLARYGSGKPIPDKGIHVIP
                     AGDSLVVELPGGGGFGDP"
BASE COUNT          492 a          920 c          829 g          433 t
ORIGIN      
        1 atccccgtga tcgagatggt cgagatcggt gccggcggcg ggtcgatcgc acgcgtcgac
       61 aggctgggcc gcatcaccgt gggtcccgac agcgcaggat cgacgcctgg gcctgtctgc
      121 tacgggcgcg gcggaaccga accggccgtg accgatgcca atctggcgct cggcaaggtc
      181 gaccctgcct atttcgccgg cggcaagatc gcgctcgatg agaagagcgc ccgccaggcc
      241 ttgcagaaga acgtcggcga caagctcgcc ctgaaggatt tctggcccgc ggccggcgtg
      301 accgagatcg tggaagagaa catggcgtat gccgcgcgcg ttcatgccat cgaactgggc
      361 cgcgacatcg cggcctgcac catgatcgcc ttcggcggcg gcgcgccgct gcatgcctgc
      421 cggctcgcag aaaaagtcgg cgtcacgacg gtgatcgtgc ccaagggcgc gggtgtcggc
      481 tccgccatcg gcttcctgcg tgcacccgtg gcctatgaaa tcacccggag tgcggtgacc
      541 tcgctcgaaa gcttcgatgc ccggcgggtc aatcggctgc tcgatagcat gacgcgcgac
      601 gcccgcgccg tggtggaacc cgcactcgct ggagcaaagg ccagcgttca gatcatcgcc
      661 gattgccgct atgtcggaca gggccatgag atccgggtaa gcgtacctgt ccgcaagttg
      721 agcgacgccg acggcaagag actgaaggcc gccttcgaga agacctatga gcaggtctac
      781 gggcttcgta ttcccaacca ggaggtggaa gccattacct ggtccgtcac cgtatcgtcg
      841 aaaacggaga agcccaggcg tgccgcaagg gccgcgcgca gggctgcgcc tcaaccccgc
      901 tcgcgccgca ccatctatga ccctgccctg ggcaagcagg tcaatgcccc ggtctactgg
      961 cgcttcgaca tgaagccggg ggccagcatc aagggaccgg ccatcatcgc ggaggacgaa
     1021 acctcgacca tcgtcggcgc ccgcttcggc gccaccatca acagcctcgg ctacatcgtc
     1081 atggagcgca ggtcatgagc aagtccgccc tcgatgcaat tcgcacgcag gtcatgtgga
     1141 accgtctcat tgcagtggtg gaggagcagg cgcagtcact tctccgcacc gctttcggca
     1201 ccatcacccg cgaggccggc gatctctcgg ccggcgtcta cgacacctcc ggcaacatga
     1261 tcgcccaggc catgaccggc acgcccggcc atgtaaacac catggcgacg gcggttgcgc
     1321 atttcttcaa gcatttcccg cagacgacca tgaaaccggg cgacgtctac gtcacgaacg
     1381 atccctggct cggcaccggc cacctcttcg actacgtggt gatgacgccg gtcttcctga
     1441 gaaggaagct cgtcgccttt ttcgcctcca cctgccatgt catcgatgtg ggcggcgtcg
     1501 gcatgtccgc caaggccaat tcctccttcg aggaaggcac gctcattccg cacatgaaga
     1561 tgcgcaagga aggcgtgctg aacgaggagc tgatcggcat catcctcgcc aattcgcgca
     1621 atccggtaga agttcggggc gacctgctct cgctcatcag cgccaacgac accggcgccc
     1681 gccgcctgct gtcgatgatg gaagaattca acctctcctc cattgaaccg ctggcccgcc
     1741 acatcctgac gcagtccgag aagggcgcgc gggaggccat caaggcgctg ccggacggcg
     1801 agtggaccta tgacatgccg ctcgacggct acgagcggcc gatcctcatc aagtcgaagc
     1861 tcaccatccg ccgcggcaag atcaccatcg acttctccgg ttcgtcgccc gcctccatcc
     1921 acggcatcaa cagcccgcgc acctataccc atgcctattc cgtcttcggc ctcaaggcgg
     1981 tgatcgcgcc gcatgtgccc aacaatatcg gctcgctgtc ctgcttcgat ctcgtgaccg
     2041 aaccgggtac ctgtgtggac cccacccgtc catcgcccgt cacggcacgg catgtgatcg
     2101 gccagatgct ggccgactgc accttcggtt gcctcgccca ggcgctgccc ggcaaggtgc
     2161 aggccgaaag cgccggctcc atctggatcc tggggctgaa cagcgcgcat ggccgcgtgc
     2221 cggcggagga gacggcaaag gccaggtatt tcggcgtcat caacatcggc ctcggcggca
     2281 tcggcggcag gccgggcaag gacgggcttt cgaccatggc cttcccgtcg ggcgtgggta
     2341 ccattccggt cgaaatcacc gagacgcagt gcccgctcta tttcaagcgc aaggaatacc
     2401 tccccgactc gggtggcgca ggcgagttcc gcggcggact ctcgcagacc atcgagatcg
     2461 caaaccgcga ggcggcaccc ttcaccatct cggccgccac cttcgaccgc atccacaatc
     2521 cggcgcaggg gcgcgacggc ggcaggccgg ggcgaaaggg cctggcgcgc tatggatccg
     2581 gcaagccgat ccccgacaag ggcatccatg tcattcccgc cggcgacagc ctggtggtgg
     2641 agctcccggg cggcggcggc ttcggcgatc ccca
//
LOCUS       DBNGVR010000474         1728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5482292, whole genome shotgun sequence.
ACCESSION   DBNGVR010000474 DBNGVR010000000
VERSION     DBNGVR010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5482292"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1109)
                     /locus_tag="AC16A0_10035"
     CDS             complement(129..1109)
                     /locus_tag="AC16A0_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3105717.1"
                     /translation="MMFEGLSVFVLVVVAVVVFYLLRAIRMVPQGFEYTVERFGRYVR
                     TLQPGLGLIVPFIDRIGAKQNMMEQFLDIPSQEVITRDNAMVKVDGIAFYQIMDAPRA
                     AYEVHDLNNAIINLVMTNIRTVMGSMDLDNLLSQRDDINRSLLSVVDQAVQPWGVKMN
                     RIEIKDISPPRDLVESMARQMKAERDKRAAILEAEGLRQAAILRAEGEKQSVVLEAEG
                     RREAAFRDAEARERSAEAEAKATEMVSNAIATGDVQAINYFVANNYIKALEALATSPN
                     QKVLMLPVEAASVIGSIGGIAEIAREAFGGDPSKTPSRRTGVPGVPAKPQ"
     gene            complement(1171..>1728)
                     /gene="hemH"
                     /locus_tag="AC16A0_10040"
     CDS             complement(1171..>1728)
                     /gene="hemH"
                     /locus_tag="AC16A0_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3105718.1"
                     /translation="DKAYDALKDMRWQPAIRTVPPYFDDPAHIEALAISLERHLKGLG
                     WTPDLILASFHGLPQDYFMDGDPYHCHCMKTARLLAARMGLPRDKLQVVFQSRFGKAE
                     WLQPYAQQTVEGLPGKGVKNLVMIMPGFASDCVETLEEVAIGLGETFHENGGENFSAV
                     PCLNDSPESVDMMETIVRRELLGWL"
BASE COUNT          307 a          559 c          551 g          311 t
ORIGIN      
        1 cagcgatgcc cagccagatc aggaacacgc ccggcgcgaa gagctcaagg atcagcagga
       61 ctccggccag aacccaccag agccaggggc ccagatgctg catgacgttc gtcatggcgc
      121 ccgccccgtc attgcggctt ggccggaacg ccgggcacgc cggtccggcg cgatggcgtc
      181 ttgctggggt ctccgccgaa ggcctcgcgg gcgatctcgg caatgccgcc gatcgatccg
      241 atcaccgaag cggcctcgac gggcagcatc agaaccttct ggttgggcga ggtggcaagc
      301 gcctcgagcg ccttgatgta attgttggcc acgaaatagt tgatcgcctg gacgtctccg
      361 gtggcaatgg cgttgcttac catttcggtg gccttggctt cggcctcggc agagcgctcg
      421 cgcgcctcgg catcgcggaa ggcggcttcg cggcggcctt cggcctcgag aaccaccgac
      481 tgcttttcgc cctcggcacg caggatcgcg gcctggcgca ggccttcggc ctcgagaatg
      541 gcggcgcgct tgtcgcgctc ggccttcatc tggcgcgcca tggattccac cagatcgcgc
      601 ggcggcgaga tatccttgat ctcgatgcgg ttcatcttca cgccccaggg ctggaccgcc
      661 tgatcgacga ccgacagcag gctgcggttg atgtcgtcgc gctgcgacag gagattgtcg
      721 agatccatgg atcccatcac cgtacggatg ttggtcatca cgagattgat gatggcgttg
      781 ttcaggtcat gcacctcata ggccgcccgg ggcgcgtcca tgatctggta gaaggcgatg
      841 ccgtcgacct tcaccattgc gttgtcgcgg gtgatcacct cctgcgacgg gatgtcgagg
      901 aactgctcca tcatgttctg cttggcgccg atgcgatcga tgaagggcac gatgaggccc
      961 agtccgggct gcagcgtccg gacatagcgg ccgaagcgtt cgaccgtgta ctcaaaaccc
     1021 tgaggcacca tccggatggc gcgcagcagg tagaagacca ccaccgccac caccaccagc
     1081 acgaagacgc tgagaccttc gaacatcact gattcctccg cctgagcccg cccataaatg
     1141 gccctgcgga cggggtttcg caagtctccg tcagagccag cccagcagct cgcggcggac
     1201 gatggtttcc atcatgtcga cggattcggg agaatcgttc agacagggga ccgccgagaa
     1261 gttctcgccc ccgttctcgt ggaaggtctc gcccaggccg atggccactt cctccagcgt
     1321 ttcgacacag tcggaggcaa agcccggcat gatcatcacg aggttcttca cccccttgcc
     1381 cggcaggccc tcgaccgtct gctgggcata gggctgaagc cattccgcct tgccgaagcg
     1441 cgactggaag accacctgca gcttgtccct gggcaggccc atcctggccg ccagaagccg
     1501 cgccgtcttc atgcagtggc aatgataggg atcgccgtcc atgaagtagt cctgcggcag
     1561 gccgtggaac gaggcgagga tgagatccgg cgtccagccg aggcccttca ggtggcgctc
     1621 gagcgaaatc gccagggcct cgatgtgggc cggatcgtcg aaatagggcg gcacggtgcg
     1681 gatcgccggc tgccagcgca tgtccttcaa ggcgtcatag gccttgtc
//
LOCUS       DBNGVR010000475         3090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1555875, whole genome shotgun sequence.
ACCESSION   DBNGVR010000475 DBNGVR010000000
VERSION     DBNGVR010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1555875"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="AC16A0_10045"
     CDS             <1..1247
                     /locus_tag="AC16A0_10045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3105719.1"
                     /translation="AEVSVADYHHMYYCYKPAEQHEVGRGLRKSALYDKLKAEGAQFA
                     QIFGWERPRWYDRSGKGEAYSFRRSNWWDAVKAEALAVRESVGLMDLSTFSKYDVKGK
                     DAHAFLERICANRIPAKDGGIILGHLLNANGFIESEITVTRLGPEHFYVLSAAVAQLH
                     DLDQLNWRKRDGEAVTVTDITDAYGVLVLAGPRARDVLSQCTDTDLTNASFRWLTGKE
                     ATVAGVPGVRLLRVNYVGELGWELHVPMAQMPTVYDALMAAGKPFDIRLFGTYAMNSL
                     RMEKSYRGWGSELTTEIDMIEASMERFLRLDKPDFIGKAPTLVNKQRGPRMKLVYMEV
                     DNTDSDCMGNEPVYHGGKVVGVTTSGAFGHKVGKSLAFAYVDPKLAAEGTEFDVMVFT
                     ETRRARIIPESIWDAENARLRA"
     gene            1280..2179
                     /locus_tag="AC16A0_10050"
     CDS             1280..2179
                     /locus_tag="AC16A0_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3105720.1"
                     /translation="MTSRTSQPLIGIGLMLSAMAILPFLDVVAKFLGQQGVPILQIVW
                     ARLFFGTLMTLPFALSLAGARGLVPNMPAMHAVRASFLIAATGFFFWGLTYLPIADCL
                     SIFFVQPLIVTMLSPLVLGEQVGIRRWMAVIIGFIGTLIIIRPGFQELNPGVFMALAA
                     GLSLAIYMLLTRRISGSAPAMVTTFYTSLMGAAIMSVLVIFVWQPVSLEQWGLFALLS
                     FFANGGHYLIVKAYDHAEASLLAPLAYTEMIMAVAAGWYFFGDFPDLWTFVGVGILIA
                     CAIYISIRETARNIAPVRDFEQP"
     gene            complement(2176..2910)
                     /locus_tag="AC16A0_10055"
     CDS             complement(2176..2910)
                     /locus_tag="AC16A0_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105721.1"
                     /translation="MNLLRCLVLMCLALPAIGLASPQASAREVLIGSTSYEARPIRDT
                     INIGGREGQFRGLRFDVRQSDVEVLEFRVVYGNGSSEDFRVRQFFRAGTSSRVFDLIG
                     RQRAIRQIIVTYVPSGPARIQFYGVEGGGGGGVIANWEQLGCKSVGFLIDRDVIQVGR
                     QDGRFRALRLEVSESPVEFFDLRVVFGNGVKQDIRVRELIRPGERTRPLDLFGDNRGI
                     ARVEMVYRSIPGFKGKSRVCVSGLSR"
BASE COUNT          550 a         1036 c          955 g          549 t
ORIGIN      
        1 cggccgaagt ctcggtcgcc gactatcatc acatgtatta ttgctacaag ccggcggagc
       61 agcacgaggt gggccgcggc ctgcgcaagt ccgcactcta cgacaagctg aaggccgagg
      121 gcgcgcagtt cgcacagatc ttcggctggg agcggccccg ctggtacgac aggagcggca
      181 aaggcgaggc atactccttc cgccgctcga actggtggga cgcggtgaag gcggaagcgc
      241 tggccgtgcg cgagagcgta ggcctgatgg acctctcgac cttctccaag tacgacgtga
      301 agggcaagga tgcccatgcc ttcctcgaac gcatctgtgc caacaggatt ccggcgaagg
      361 atggcggcat catcctcggg catctgctga atgccaatgg cttcatcgaa tccgagatca
      421 ccgtcacgcg gctggggccg gaacacttct acgtgctctc ggccgcggtg gcgcagctgc
      481 atgacctcga ccagctgaac tggcgcaagc gcgacggcga ggccgtgacc gtcaccgaca
      541 tcaccgacgc ctatggcgtg ctggttctgg ccggccccag ggcgcgcgac gtgctgagcc
      601 aatgcaccga taccgatctc accaatgcga gcttccgctg gttgaccggc aaggaagcaa
      661 ccgtcgccgg cgtccctggc gtgcgcctgc tgcgcgtcaa ctatgtgggc gaactgggct
      721 gggaactgca tgtgccgatg gcgcagatgc ccacggtcta tgatgccctg atggcggcgg
      781 gcaagccctt cgacatccgt ctcttcggca cctatgcgat gaactcgctc cgcatggaga
      841 agtcctaccg cggctggggc tcggagctca ccaccgagat cgacatgatc gaggcttcga
      901 tggagcgctt cctgcgcctc gacaagccgg atttcatcgg taaggcgccg acgctggtga
      961 acaagcagcg cggaccgcgc atgaagctgg tctacatgga agtcgacaac accgactccg
     1021 actgcatggg caacgagcca gtctatcacg gcggcaaggt ggtgggcgtc accacctccg
     1081 gcgccttcgg gcacaaggtc ggcaagtcgc tggccttcgc ctatgtcgat ccgaagctgg
     1141 cggccgaggg cacggagttc gacgtcatgg tcttcaccga gacgcgcagg gcccgcatca
     1201 tccccgagtc gatctgggat gccgagaatg caaggctgag ggcctagcgc cgcggcgcgg
     1261 gcaccgctaa agcccggcca tgacgtccag gacctcgcaa ccgctgatcg gcatcggcct
     1321 gatgctctcg gccatggcca tcctgccctt cctcgacgtg gtggccaagt tcctcggcca
     1381 gcagggtgtg ccgatcctgc agatcgtgtg ggcgcggctc ttcttcggca cgctgatgac
     1441 attgcccttc gcgctgagcc tcgccggcgc gcggggcctc gtccccaaca tgccggcgat
     1501 gcatgcggtg cgcgccagct tcctcattgc cgccaccggc ttcttcttct ggggcctcac
     1561 ctacctgccg atcgccgact gcctgtcgat cttcttcgtc cagcccctca tcgtcaccat
     1621 gctgtcgccg ctggtgttgg gcgaacaggt cggcatccgg cgctggatgg ccgtcatcat
     1681 cggattcatc ggcacgctga tcatcatcag gcccggattc caggagctga accccggcgt
     1741 cttcatggcc cttgccgccg gactgtcgct ggccatctac atgctgctga cccgccgcat
     1801 ctccggcagc gcgccggcca tggtaaccac cttctacacc agcctgatgg gcgcggcgat
     1861 catgtccgtc ctcgtcatct tcgtgtggca gccggtgagt ctcgagcaat ggggtctctt
     1921 cgccctgctc tcctttttcg ccaatggcgg gcactacctg atcgtcaagg cctacgacca
     1981 cgccgaggct tcgctcctgg cgccgctggc ctatacggag atgatcatgg cggtggccgc
     2041 cggttggtac ttcttcggcg actttcccga tctctggacc ttcgtcggcg tcggcatcct
     2101 gatcgcctgc gccatctaca tctcgatccg cgaaacggcg cgcaacatcg cgccggtccg
     2161 cgatttcgag cagccctagc ggctcagccc gctgacgcag acgcgggact tgcccttgaa
     2221 gccggggatg gagcgataga ccatctcgac ccgcgcaatg ccgcgattgt cgccgaagag
     2281 atcgagcgga cgcgttcttt cgccgggccg gatcagttcc ctcacgcgga tgtcctgctt
     2341 gacgccattg ccgaagacca cgcgaaggtc gaagaactcg accggcgact cggagacttc
     2401 cagccgcagc gcccggaagc ggccgtcctg acggccaacc tgaatgacat cgcgatcgat
     2461 caggaagccc acgctcttgc agccaagctg ctcccagttg gcaatgacgc caccgccgcc
     2521 gccaccttcc acgccataga actggatgcg cgcagggccg gacggcacgt aggtgacgat
     2581 gatctggcgg atggcgcgct gccgcccgat gagatcgaaa acgcgcgagg acgttcccgc
     2641 gcggaagaac tggcgcaccc tgaagtcttc gctggaccca ttgccataga cgacgcggaa
     2701 ctccagcact tcgacatcgc tctgacgcac atcgaaacgc aggccacgga actggccttc
     2761 ccggccacca atgttgatcg tgtcgcggat cggccgcgcc tcgtaactgg tgctgccgat
     2821 gagcacctcg cgggcggacg cctgcggcga tgccaatccg atggccggga gggccagaca
     2881 catgagaacc agacagcgga gcagattcac gggtttctcc ttcgtcattg caacaagctc
     2941 atcaccaaaa cagttgcggc gcgccaggcc gcatcagact tcacgggcca cgccctcgac
     3001 aagcggcacg aagcgcaccg gcagaagatg ttcctggtca tagcccttgg ccgtgcgggt
     3061 tatgcggaag agatcctgct ttccgggccg
//
LOCUS       DBNGVR010000476         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3323176, whole genome shotgun sequence.
ACCESSION   DBNGVR010000476 DBNGVR010000000
VERSION     DBNGVR010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3323176"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1396)
                     /locus_tag="AC16A0_10060"
     CDS             complement(113..1396)
                     /locus_tag="AC16A0_10060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR3105722.1"
                     /translation="MAAILLRNVTIWDGENDAAYPGEVLIEGNRIKAVARARNEIAAE
                     RASDIIDGEGMFLMPGMVEGHCHPSFVGISTNAELGRIPPEKHTLLTAANVKLLLEHG
                     FTSIFEAASAKPMIGVTIRDAINDGLIPGPRMRAASPEVTTTAGLGDERRRHIHEESF
                     GLVADGPIEMRRVARECARDGVDNLKINISGDEFVSHARAEITTLEDDELQAFVSVAH
                     NFGKMVAAHARSSKSVKMAVRHKVDCIYHCDFADEEALDLLESVKDESFVGPAFGLVH
                     NCTIEGERYGFSRELAESMHLFRKFEATCATYHEIRRRGIRVVIGGDYGFSITPMGQN
                     ARDIGHFVKYFGYSPAEALRCATRVGAELMGHGGELGVIREGALADLLLVDGNPLADQ
                     SVLVGPDRFRMIMKDGSLYRDPRQAGGRRQQVAAE"
     gene            complement(1409..>1975)
                     /locus_tag="AC16A0_10065"
     CDS             complement(1409..>1975)
                     /locus_tag="AC16A0_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105723.1"
                     /translation="VVQGLMVLHTGGFAPQDHATDAMHFLAVKRSIFGIPNALFVWLF
                     VGLIVIFLLRRTAFGRAVYAIGNRESVAYLSGINTNAVLIGCFAFCGACAAFAGVLLA
                     GYSTKAYQAMGDPYLLPAIAAVVLGGTNILGGRGNYLGTIAGVILITLLQSILSVMQM
                     PEAGRQVIYGSVIIGMLLLYGRGARTRS"
BASE COUNT          354 a          619 c          660 g          342 t
ORIGIN      
        1 ccgcccatgg catcgacaag gaagctcgcc ggcgcgtccc cgaaggcagc gatcaccgaa
       61 tcggccacgc ggcggggttt gcgcgcgatt gtcaggagcg gcggatcctc gatcactcag
      121 cggcaacctg ctggcgacgg ccgccggcct ggcgggggtc acggtagagg gatccatctt
      181 tcatgatcat gcggaaacgg tccggcccga ccagtaccga ctggtctgcc agcggattgc
      241 cgtcgaccag cagcagatcg gcaagggcac cttcgcggat cacgccgagt tcgcctccat
      301 ggcccatcag ctcggcgccg accctggtgg cgcagcgcag cgcctcggca ggcgagtagc
      361 cgaagtactt cacgaagtgg ccgatgtcgc gggcgttctg gcccatcggc gtgatcgaga
      421 aaccgtagtc gccgccgatc accacgcgga tgccgcggcg gcggatctcg tggtaggtgg
      481 cgcaggtcgc ctcgaacttg cggaacagat gcatggactc cgccagttcg cggctgaagc
      541 cgtagcgttc gccctcgatg gtgcagttgt gcaccaggcc gaatgccggg cccacgaagg
      601 actcatcctt cacgctttcg agcaggtcga gcgcttcctc atcggcgaag tcgcagtggt
      661 agatgcagtc caccttgtgg cgaaccgcca tcttcacgct cttggacgat cgcgcatggg
      721 cggcgaccat ctttccgaag ttatgcgcca cgctgacgaa ggcctgcagc tcgtcgtcct
      781 ccagcgtggt gatctcggcg cgggcgtggc tgacgaactc gtcgcccgag atgttgatct
      841 tgagattgtc cacgccgtcg cgcgcgcatt cccgcgccac gcgccgcatc tcgatgggtc
      901 cgtcggcaac caggccgaag ctctcttcat ggatgtggcg gcggcgttcg tcgcccaggc
      961 cggcggttgt cgtgacttca gggcttgcgg cccgcatgcg cgggccgggg atgagcccat
     1021 cgttgatggc atcgcggatg gtgacgccga tcatgggttt ggccgaggcc gcctcgaaga
     1081 tcgaggtgaa gccgtgttcg agcagcagct tcacattggc ggcggtgagc agtgtgtgct
     1141 tctcgggcgg aatacggccc agttcggcgt tggtggagat tccgacgaag ctcgggtggc
     1201 aatgtccttc caccatgccc ggcatgagga acatgccttc gccgtcgatg atgtcggagg
     1261 cccgctcagc cgcgatctcg ttgcgcgccc tggcgacggc cttgatgcgg ttgccttcga
     1321 tcagcacttc gccgggatag gccgcatcat tctcgccatc ccagatcgtc acattccgca
     1381 acaggatcgc agccattctt tcctcccgtc aactccgtgt gcgcgcgcct cgtccgtaaa
     1441 gaagcagcat gccgatgatc accgacccgt agatcacctg gcggccagcc tcgggcatct
     1501 gcatgaccga gagaatggac tgcagcaggg tgatcaggat gacaccggca atggtgccca
     1561 gatagttgcc gcgcccgccc aggatgttgg tgccgccaag tacgacggcg gcaatggcgg
     1621 gcagcagata ggggtcgccc atggcctgat aggccttggt cgagtagccg gccagcaaca
     1681 cgcccgcgaa ggccgcgcag gcgccgcaga aggcaaagca gccgatcagc acggcgttgg
     1741 tattgatacc agacagatag gcgacggact cgcggttgcc gatggcatag acggcgcggc
     1801 cgaaggcggt gcgccgcagc aggaagatca cgataaggcc gacgaagagc cagacgaaga
     1861 gggcgttggg aatgccgaag atcgagcgtt tcaccgccag gaaatgcatg gcgtcggttg
     1921 catggtcctg cggcgcgaag ccgcccgtgt gcaataccat gaggccctgt accac
//
LOCUS       DBNGVR010000477         3646 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4697334, whole genome shotgun sequence.
ACCESSION   DBNGVR010000477 DBNGVR010000000
VERSION     DBNGVR010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4697334"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16A0_10070"
     CDS             complement(<1..466)
                     /locus_tag="AC16A0_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR3105724.1"
                     /translation="MALASRRRRAEDAPYWPGFVDAMAQLLLVITFLLSVFMIGQFLL
                     ARQISGQDSALGQLRSQIAELTQLLALEKSAKAEVEATLGALQDDLAAEKAKAEDLSA
                     MLASESEKGSSAGSALAGLRTALDKEKTISADALAKVELLNQQIAAMRRQLQT"
     gene            complement(466..1506)
                     /locus_tag="AC16A0_10075"
     CDS             complement(466..1506)
                     /locus_tag="AC16A0_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HIR3105725.1"
                     /translation="MADTTTAAPLEPPRVYLVHMIVFTLVVAVLVAVLLPGMVNAFMA
                     NPFLNGVIIGTLLLGMFHSYRMVWRLFPEVNWVNRFRESDAESLRQPQLLGPMATLLR
                     NRQTAMLSPLSMRSLLDSLASRLDESRDLSRYMVGLLIFLGLLGTFWGLLETVTSVGD
                     AIRALDVTAAQSSTMFDELKRGLEKPLAGMGLSFSSSLFGLAGSLILGFLDLKAAQAQ
                     NRFYNDLEDWLSTFTDLAAGDPQSQGSTAPQFLRLDLQSLQRSIEKLNGSIEHNKATA
                     ATGGPGNSEAMEQLAEAVAGLVSQMREEQKTVRQWAQSQQTQQNEIQRLLLRVTGPAQ
                     RTTGRARSEDER"
     gene            complement(1585..2976)
                     /locus_tag="AC16A0_10080"
     CDS             complement(1585..2976)
                     /locus_tag="AC16A0_10080"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR3105726.1"
                     /translation="MSANLAALLYLVSGALFIMALRGLSSPATSRMGNIYGMVGMTIA
                     MLTTLAMMDLSRMVLALTAAGLAIGGGIGAYAARNIAMTAMPQLVAAFHSLVGLAAVF
                     VAAAAFYAPDAFHIVQDGLIMPLSRIEMSLGAAIGAITFTGSIIAFLKLDGRMSGAPI
                     LLPARHVLNIALGAALVLCFVLFYANPSWIWFWLIILISLALGFLLIIPIGGADMPVV
                     VSMLNSYSGWAAAGIGFTLHNTALIITGALVGSSGAILSYIMCKGMNRSFISVILGGF
                     GGESAGPAAKGEAKPVKQGSAEDAAFVMKNAGSVIIVPGYGMAVAQAQHALREMADHL
                     KKEGVSVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSDFGQADVAFVIGAN
                     DVTNPSAKDDPTSPIYGMPVLEVWKAKTVMFIKRGMSSGYAGVDNPLFWRDNTMMLFG
                     DAKKMTEEIVKSM"
     gene            complement(2980..3297)
                     /locus_tag="AC16A0_10085"
     CDS             complement(2980..3297)
                     /locus_tag="AC16A0_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HIR3105727.1"
                     /translation="MEQIAHSVDPFVSLFGIFVLAVFVGYYVVWNVTPALHTPLMSVT
                     NAISSVIVVGALLAVGVGALASGSVVAMIFGFLALILASVNIFGGFMVTSRMLAMYKK
                     KDK"
     gene            complement(3297..>3646)
                     /locus_tag="AC16A0_10090"
     CDS             complement(3297..>3646)
                     /locus_tag="AC16A0_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HIR3105728.1"
                     /translation="PGRPAPVLITKDMVESMKPGSVIVDLAVERGGNCPLSRPDEVVV
                     HNGVSIVGTVNLAGKLAGNASPLFAKNLANFLDLMVQKDGSLKIDEADECIKGTMIAK
                     GGELVHAMFTGAK"
BASE COUNT          699 a         1138 c         1220 g          589 t
ORIGIN      
        1 gggtctgcaa ctggcggcgc atggcggcga tctgctggtt cagcaattcg accttggcca
       61 gggcatcagc cgaaatggtc ttctccttgt cgagcgcggt gcgcaggccc gcgagcgccg
      121 aacctgcgga ggagcccttc tcgctttcgc tggcaagcat ggccgacaga tcctcggcct
      181 tcgccttctc tgcggcaaga tcgtcctgca gcgcgccgag ggttgcctcc acctcggcct
      241 tggcggattt ttccagcgcc agcagttgcg tgagctcggc gatctgcgag cgcagctggc
      301 ccagagccga atcctggccc gagatctgac gcgccagaag gaactgcccg atcatgaaga
      361 ccgacagcag gaaggtgatg accagaagca actgggccat ggcatcgacg aagccgggcc
      421 agtagggcgc atcctctgcc ctgcggcgcc gcgatgccag tgccatcagc gctcgtcctc
      481 gctacgcgcg cggccggtgg tgcgctgcgc cggaccggtg acacggagca gcaggcgctg
      541 gatctcgttc tgctgggtct gctggctctg cgcccactgg cgcacggtct tctgctcctc
      601 gcgcatctgg gacacgaggc cggccacggc ttccgccagc tgctccatcg cctcgctgtt
      661 gccaggcccg ccggtcgccg cggtcgcctt gttgtgctcg atcgaaccgt tgagcttctc
      721 gatgctccgt tgcagcgact gcaggtcaag gcgcaggaac tgcggcgcgg tagagccctg
      781 cgactgcgga tcgccggctg ccagatccgt gaaggtcgaa agccagtctt cgagatcatt
      841 gtagaagcgg ttctgcgcct gtgcggcctt caggtcgagg aagccgagga tcagcgatcc
      901 ggcgaggccg aagagcgacg aggaaaagga caggcccatg ccggccagcg gcttctccag
      961 tccgcgcttc aactcgtcga acatggtgct cgactgtgcg gccgtcacgt cgagcgcgcg
     1021 aatggcatcg cccaccgagg tgacggtttc gagcaggccc cagaaggtgc cgagcaggcc
     1081 gaggaagatc aggaggccca ccatgtagcg cgagagatcc cgcgattcat cgaggcgcga
     1141 ggccagcgag tcgagcagcg aacgcatcga caggggcgac agcatcgcgg tctgccggtt
     1201 gcgcagcagg gtcgccatgg ggcccagcaa ttgcggctgg cgcaggctct cggcatccga
     1261 ctcgcggaaa cggttgaccc agttcacctc gggaaagagg cgccagacca tgcgatagga
     1321 atggaacatg ccgagcagca gcgtgccgat gatgacgccg ttgaggaagg gattggccat
     1381 gaaggcattg accatgcccg gcaggaggac ggccacgaga accgccacca ccagggtaaa
     1441 gacgatcatg tggacgagat agacccgcgg aggttccagc ggtgctgccg tggtggtgtc
     1501 tgccatctgc tgcctcaacc ccctgctctt ggcggcaaat ctagaccaat acgtggcgga
     1561 gcgggagggg ttctcccgcc gccgctacat cgacttgacg atctcctcgg tcatcttctt
     1621 ggcgtcgccg aaaagcatca tggtgttgtc gcgccagaac agcgggttgt cgacaccggc
     1681 atagccggac gacatgccgc gcttgatgaa catcacggtc ttcgccttcc acacctcgag
     1741 caccggcatg ccgtagatgg gagaggtcgg gtcgtccttg gccgacggat tggtcacgtc
     1801 attggcgccg atgacgaagg cgacatcggc ctggccgaag tccgagttga tatcctcgag
     1861 ctcgaagacc tcgtcatagg gcacgttcgc ttctgccagc agcacgttca tgtgtcccgg
     1921 catgcggccc gccacgggat ggatggcgta tttcaccgaa acgccttcct tcttgaggtg
     1981 gtcggccatt tcgcggagcg cgtgctgcgc ctgggcgacg gccatgccat agcccggcac
     2041 gatgatgacc gagccggcat tcttcatcac gaaggcggcg tcctcggccg agccctgctt
     2101 caccggcttg gcttcgccct tggcggcagg cccggcactc tcgccgccga agccgccgag
     2161 aatgacggag atgaacgacc gattcatgcc cttgcacatg atgtaggaca ggatcgcgcc
     2221 ggacgaaccc acgagcgcgc cggtaatgat cagtgccgtg ttgtgcaggg tgaagccgat
     2281 gccggcggcg gcccagcccg aataggaatt cagcatcgac acgaccaccg gcatgtcggc
     2341 gccgccgatc gggatgatga gcaggaagcc cagcgccagg ctgatcagga tgatgagcca
     2401 gaaccagatc cagctggggt tggcatagaa gagcacaaag cagaggacga gcgccgcgcc
     2461 cagcgcgatg ttgagcacgt gacgcgccgg cagcaggatc ggcgcgccgg acatgcggcc
     2521 gtcgagcttg aggaaggcga tgatcgagcc ggtgaaggtg atggcaccga tggccgcgcc
     2581 gagcgacatc tcaatgcggc tcaacggcat gatcagcccg tcctgcacga tatggaaggc
     2641 atcgggcgca tagaaggccg cagccgcgac gaagaccgcc gcgaggccga ccagcgagtg
     2701 gaaggcggcg accagctgcg gcatggccgt catggcgatg ttcctggcgg cataggcgcc
     2761 gatgccgccg ccgatcgcca gaccggcggc cgtcagggcc aggaccatgc gcgacagatc
     2821 catcattgcc agcgtcgtca gcatggcgat ggtcatgccg accatgccgt agatgttgcc
     2881 catgcgcgag gtggcgggcg aggaaagccc gcgcagcgcc atgatgaaga gcgcgccgga
     2941 aaccagatag agaagggctg cgagattcgc cgacatcgcc tacttgtcct tcttcttgta
     3001 catggcgagc atgcggctcg tgaccatgaa gccgccgaag atgttgaccg aggccaggat
     3061 cagggcgagg aagccgaaga tcatggcaac gaccgagccc gaggccagcg cgccgacacc
     3121 caccgcaagc agcgcgccga ccacgatgac cgacgagatg gcgttggtca ctgacatcag
     3181 cggcgtatgc agtgccgggg tgacgttcca gaccacgtag tagccgacga agacggccag
     3241 cacgaagatg ccgaacagcg agacgaaggg gtcaaccgaa tgggcgatct gttccatcac
     3301 ttcgctcccg tgaacatggc atgcaccagc tcgccgccct tggcgatcat ggtgcccttg
     3361 atgcattcgt cggcctcgtc gatcttgagg ctgccgtcct tctggaccat gaggtcgagg
     3421 aagttggcga ggttcttggc aaagagcggc gaggcattgc ccgccagctt gccggcaaga
     3481 ttgacggtac cgacgatcga cacgccgttg tggacgacga cttcgtccgg ccgcgacagc
     3541 gggcagttgc cgccgcgctc gaccgcgaga tcgacgatca ccgaaccggg cttcatcgac
     3601 tcgaccatgt ccttggtgat cagcaccggg gccggacggc cgggga
//
LOCUS       DBNGVR010000478         2846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_969577, whole genome shotgun sequence.
ACCESSION   DBNGVR010000478 DBNGVR010000000
VERSION     DBNGVR010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_969577"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC16A0_10095"
     CDS             complement(<1..256)
                     /locus_tag="AC16A0_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR3105729.1"
                     /translation="MRRKAVLLISTPVMSVTPRRTTRRARSLRRAPSEAEKRLWGSLR
                     GRRLKDCKFVRQAPLGPYFADLLCRERKLIVEIDGVTQGED"
     gene            complement(404..1291)
                     /gene="aguB"
                     /locus_tag="AC16A0_10100"
     CDS             complement(404..1291)
                     /gene="aguB"
                     /locus_tag="AC16A0_10100"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HIR3105730.1"
                     /translation="MRKITAAITQMASTQSWAGNCDKAEALVRQAAAQGAGLVLLQEL
                     FDADYFCIEQHVRFFAGAHELDQHPTALRFGALARELGVVLPVSVFERAGPAHYNTTV
                     IFDADGTNLGFYRKSHIPDGRGYQEKFYFSPGDTGFKVWDTAVGRIGLGICWDQWFPE
                     AARVMALMGAEMLLYPTAIGSEPGSPDYDSSNHWQNTMRGHAAANIMPLLASNRIGRE
                     LAPDGRSDTFYGRSFIADPHGEKVAEMSRTEEGLRLASFDLDEIAELRRTWGVFRDRR
                     PELYGTLLTIDGRTRRDGL"
     gene            complement(1293..>2846)
                     /locus_tag="AC16A0_10105"
     CDS             complement(1293..>2846)
                     /locus_tag="AC16A0_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3105731.1"
                     /translation="GAHGFGVIQHFLSPRPNQRTDEYGGSLANRARLRKELISDARDE
                     VGDTCAIAVRLSLDEMAGPSGFTNAELRDLVAMHAELPDLWDFAHGTWEACSGTSRFK
                     PEGAQEDLIRGIKQLTPKPVVGVGRFTSADMMVRQVKQGILDFVGAARPSIADPFLPR
                     KIDENRIEDIRECIGCNICVTGDMTMSLSRCTQNSSFMEEWRKGWHPERMPAKKTDTR
                     VLVIGAGPAGLSAAHRVGLRGYEVALAEAGTELGGRVARECRLPGLSAWGRVRDYRAG
                     QIGKMTNVNVYRDSRLTAEDVLGFGFEHVAVATGATWRRDGIARYNLLPIAIAPAMPV
                     FTPDDIMDGKRPSGPVVVYDDDHYYMGSVLAELLVQAGCRVTFLTPAARAAEWSFNTL
                     EQGLIQARLIESGIDIRTNRGLAAIAAGGVTAACSYTGRESEIACDAVVLVTARLPED
                     GLYLDLTARAAEWADHGLRSVKLVGDANAPAAIAWATYAGHRYAEELDAPDRGDALPF
                     RREVATLKD"
BASE COUNT          450 a          931 c         1001 g          464 t
ORIGIN      
        1 gatcttcgcc ttgagtcacg ccatcgatct cgacgataag ttttctctcg cggcacagga
       61 ggtcggcaaa gtatgggccg agcggtgcct gcctgacgaa cttgcaatct ttgagacggc
      121 ggccccgcag agaaccccaa agccgcttct cggcttcgct cggcgcccgc cgcaagctcc
      181 gcgcgcgccg tgtcgtcctg cggggcgtga cggacatgac gggggtggag attagcagca
      241 ccgccttgcg gcgcaaggcg gccctcatcc ggcgctgcgc gccaccttct cccgcaggcg
      301 ggagaagggt ccaaacaagt cgtccgtctc ccgcaggcgg gaaaagggtc gtgtacggat
      361 catccttctc ccgctcgcgg gagatggggg cttatggttg agcttacagc ccgtctcggc
      421 gggtgcggcc gtcgatggtg aggagcgtgc cgtaaagctc cggccgccgg tcgcggaaga
      481 cgccccaggt gcggcggagc tccgcgatct cgtcgaggtc gaagctggcc agccgcaggc
      541 cctcctcggt gcggctcatc tccgcgactt tctcgccgtg cggatcggcg atgaaggagc
      601 ggccgtagaa ggtgtcgctc cgcccgtcgg gggccagctc gcggccgatg cggttggagg
      661 cgagcagcgg catgatgttg gccgccgcat ggccgcgcat ggtgttctgc cagtgattgc
      721 tggaatcata gtcggggctg ccgggttccg acccgatggc ggtgggatag agcagcatct
      781 ctgcgcccat cagcgccatg acgcgggccg cctcggggaa ccactggtcc cagcagatgc
      841 cgaggccgat gcggcccacc gccgtgtccc agaccttgaa gcccgtgtcg cccggcgaga
      901 agtagaactt ctcctgatag ccgcgcccgt ccggaatgtg actcttgcgg tagaagccga
      961 gattcgtgcc gtcggcgtcg aaaatcaccg tggtgttgta atgcgccggc cccgcccgct
     1021 cgaagacgct gaccgggagc accacgccaa gctcgcgcgc cagggcgccg aagcgcagag
     1081 cagtcggatg ctggtccagc tcatgggcgc cggcgaagaa gcggacatgc tgctcgatgc
     1141 agaagtaatc ggcatcgaag agttcctgca gcagcacgag gcccgcaccc tgtgctgcgg
     1201 cctggcgcac cagggcttcg gccttgtcgc agtttccggc ccagctctgg gtcgaggcca
     1261 tctgggtgat ggcggcggtg atcttgcgca tgtcagtcct tcagggttgc cacttcgcgg
     1321 cggaacggca gggcatcgcc gcggtccggc gcatcgagtt cctcggcata gcggtggccg
     1381 gcataggtgg cccaggcgat ggcggcgggc gcattggcgt cgccgacaag cttcaccgac
     1441 cggaggccgt gatcggccca ctccgcggcg cgggcggtga gatcgaggta gagcccgtct
     1501 tcgggcagtc ttgcggtgac cagcaccacc gcgtcgcagg cgatctcgct ctcgcggccg
     1561 gtatagctgc aggccgcggt gacgccgccg gcagcaatgg cggcaagacc gcgattggtg
     1621 cggatatcga tgccggattc gatgaggcgg gcctggatca gcccctgctc gagcgtgttg
     1681 aagctccact ccgccgcgcg cgccgcgggc gtgaggaagg tgacgcggca gccggcctgc
     1741 accaggagtt cggccagcac gctgcccatg tagtagtggt cgtcgtcata gaccaccaca
     1801 ggcccggagg gccgcttgcc gtccatgatg tcgtcgggcg tgaagaccgg catggcggga
     1861 gcgatggcga tgggcaggag attgtagcgc gcgatcccgt cgcgccgcca tgtggccccg
     1921 gtggccaccg ccacatgctc gaatccgaaa cccaggacat cctcggcggt gagccgcgag
     1981 tcacgataga cattcacgtt ggtcatcttg ccgatctggc ccgcgcggta gtcgcgcacc
     2041 cggccccagg cggaaagccc gggcaggcgg cactcgcgcg ccacgcggcc gccgagttcg
     2101 gtgcccgcct cggccagcgc cacctcgtag ccgcgaagac ccacgcgatg cgccgccgaa
     2161 aggccggcag gcccggcgcc gatcaccagc acgcgggtgt cggttttctt cgccggcatg
     2221 cgctcgggat gccagccctt gcgccattcc tccatgaagg aggagttctg tgtgcagcgg
     2281 ctgagcgaca tggtcatgtc gccggtcacg caaatgttgc agccgatgca ttcgcggatg
     2341 tcctcgatgc ggttctcgtc gatcttcctg ggcaggaagg gatcggcgat ggacggccgc
     2401 gcggcgccga cgaagtcgag gatgccctgc ttcacctggc gcaccatcat gtcggccgag
     2461 gtgaagcggc cgacgcccac caccggcttc ggcgtcagct gcttgatgcc gcggatcagg
     2521 tcttcctgcg cgccttccgg cttgaagcgc gaggtgccgg aacaggcttc ccaggtgcca
     2581 tgggcgaaat cccagagatc gggaagctcg gcatgcatgg cgacgaggtc gcggagctct
     2641 gcattggtga agcccgaggg acccgccatc tcgtcgagcg aaaggcgcac cgcgatggcg
     2701 caggtgtcgc ccacctcgtc gcgcgcgtcc gagatcagct ccttccgcag gcgcgcgcgg
     2761 ttcgcgagcg agccgccata ttcgtccgtc cgctgattgg ggcgcggcga gaggaaatgc
     2821 tggatcacgc cgaagccatg cgcgcc
//
LOCUS       DBNGVR010000479         3925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5287057, whole genome shotgun sequence.
ACCESSION   DBNGVR010000479 DBNGVR010000000
VERSION     DBNGVR010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5287057"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1371)
                     /locus_tag="AC16A0_10110"
     CDS             complement(28..1371)
                     /locus_tag="AC16A0_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR3105732.1"
                     /translation="MELDPILLGRMQFAANITFHILFPSITIALGWILVFFKFRYHAT
                     RDMKWMDSYFFFTKVFALCFALGVVSGITMSFQFGTNWPGFMNTVGNIAGPLLAYEVL
                     TAFFLEATFLGVMLFGFRRVPGWLHTLAGVLVAFGTTLSAFWILVLNSWMHTPAGFEM
                     RDGVAHATDWFAIIFNPSMPYRLAHMLLASGLTAAFLVAGVSAYRWLKNDRSPAVSSA
                     LRTGVYLAALLIPLQIYMGDLHGLNTLEHQPQKVAAMEGLWETRKGAPLVLFGWPNAE
                     TRTNDYALEIPDLASLILTHDLKGEVKGLNDFGTDHPPVAPVFWAFRIMVGTGVLMLL
                     VSWAGAYVIRRHGLSRPLARVLVAMTFAGWVATVAGWYVTEIGRQPWLVQGVLRVADA
                     ASDGPAPMIGMSLSMYLAVYAVLLAAFIQTIFHMAGKAAKTAAPGRASSRRNRSG"
     gene            complement(1464..1871)
                     /gene="hisI"
                     /locus_tag="AC16A0_10115"
     CDS             complement(1464..1871)
                     /gene="hisI"
                     /locus_tag="AC16A0_10115"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR3105733.1"
                     /translation="MADSLDETETFQPRYDSEGLIPAIVTDSSDGTVLMFAHMNAEAL
                     RLTLDTGYAHFWSRSRQALWKKGETSGELLQVTEVLTDCDQDVLQVKARQMGRGAACH
                     TGRRSCFYRRIDKGRLVFVDQHRFFDPKSVHRS"
     gene            complement(1871..2497)
                     /gene="folE"
                     /locus_tag="AC16A0_10120"
     CDS             complement(1871..2497)
                     /gene="folE"
                     /locus_tag="AC16A0_10120"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR3105734.1"
                     /translation="MDAIIKKIPLKPVKRPSQEEAEAAVRVLLAWAGDDPDREGLLDT
                     PKRVAKAFREYFSGYEDDPAEALSRTFDDVGGYDDIVMLRDIEFNSHCEHHIAPFVGR
                     AHVAYLPTKSVVGISKLARVVEIYARRLQTQETMTAQIANAIMNALQPRGVAVLIEAV
                     HTCMSMRGIGKRNVATITTQFTGEFKSNPVLQARFMELVRAPSGGFSL"
     gene            2681..3130
                     /locus_tag="AC16A0_10125"
     CDS             2681..3130
                     /locus_tag="AC16A0_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR3105735.1"
                     /translation="MIDDIYNRKILGFAADIPRLQRLAAPDATAVAHSRLCGSKVTVD
                     LKMKDGVVTDFGHEVKACALGQASSSIMARHVVGSTAVELRDLRDQMHRMLKEEGPPP
                     GGKWSDLEALVPVRNFKARHASTLLTFDAVADAVGQIEAKGRELAHG"
     gene            3123..3596
                     /locus_tag="AC16A0_10130"
     CDS             3123..3596
                     /locus_tag="AC16A0_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3105736.1"
                     /translation="MADDRPAALRAAELPARTASIYPAAFAKALEGRSKRALGDAFDL
                     TQFGVNYTVLEPGSASSERHWHAVEDELVFVLEGEVTLIDEAGEHLLVPGMCAGFKAG
                     VANGHKLVNRGAVRAVFLEIGTRSPTESVVYPDVDMLARKAGGKYILTHKDGTPY"
     gene            3593..3856
                     /gene="yidD"
                     /locus_tag="AC16A0_10135"
     CDS             3593..3856
                     /gene="yidD"
                     /locus_tag="AC16A0_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR3105737.1"
                     /translation="MKTILLALIRAYQLTLSALIGRRCRYLPTCSDYASEAIRLHGAW
                     HGTVLGFARVCRCHPWGQSGFDPVPKVYDGPVGRMKTGAPEGP"
BASE COUNT          729 a         1206 c         1307 g          683 t
ORIGIN      
        1 gccattggcc gttggcgaga atgtccatca tccgctccta ttccgccggg atgaagcccg
       61 gccgggggcc gccgtcttgg ccgccttgcc tgccatgtgg aagatcgtct ggatgaaggc
      121 ggccagcagg acggcgtaga ccgcaagata catggaaagg ctcatcccga tcatcggtgc
      181 cggaccgtcc gaagccgcgt cggcaacccg caggacaccc tgtacgagcc agggctggcg
      241 gccgatctcc gtgacatacc agccggccac cgtcgcgacc cagcctgcga aggtcatggc
      301 aaccagcacg cgcgccagcg gccgcgacaa tccgtggcga cggatgacat aggcgcctgc
      361 ccaggagacc agcagcatca gcacgccggt gcctaccatg atccggaagg cccagaacac
      421 cggggcgacg ggcgggtggt cagtgccgaa atcattcagc cccttcactt ctcccttgag
      481 gtcatgcgtg aggatgaggc tggcgaggtc cggaatctcc agggcatagt cgtttgtcct
      541 ggtttcggcg ttgggccagc cgaagaggac gagaggcgcc cccttgcgcg tttcccaaag
      601 cccttccatg gctgccacct tttgcggctg atgctccagc gtgttgaggc cgtgcaggtc
      661 tcccatgtag atctgcaacg ggatcagcag ggccgcaagg tagacgccgg tccggagggc
      721 agaagagact gccgggctgc ggtcgttctt cagccagcgg taggccgaga cgcccgccac
      781 cagaaatgcc gcggtaaggc ccgacgccag cagcatatgc gcgaggcggt agggcatgga
      841 ggggttgaag atgatggcaa accagtcggt ggcgtgcgca acgccgtcac gcatctcgaa
      901 gcccgccggc gtatgcatcc agctgttgag cacgaggatc cagaaggccg acagcgtggt
      961 gccgaaggcg acgagtacgc cggccagcgt atgcagccag ccgggaacac ggcggaagcc
     1021 gaagagcatg acccccagga atgtcgcctc gaggaagaag gccgtcagca cctcgtaggc
     1081 cagcagcgga ccggcaatgt ttccaaccgt gttcatgaag cctggccagt tggtgccgaa
     1141 ctgaaacgac atggtgatgc cgctgaccac gccaagggcg aagcacagcg cgaagacctt
     1201 cgtgaagaag aagtaagaat ccatccactt catgtcgcgc gttgcatggt aacggaactt
     1261 gaagaagacg aggatccagc caagcgcgat cgtgatgctc ggaaagagga tatggaaagt
     1321 gatgttggcg gcaaattgca tccgcccgag gaggatgggg tcaagttcca tcagtcacca
     1381 gctttgtcgt cgttcccttg gccagaatac gcaactttcg gagcgacggg ttgcggcgcg
     1441 ccgccgcagc gcatcgaacg gcttcagctc cggtggacgg acttcggatc gaagaaccgg
     1501 tgctggtcga cgaacacgag ccgtcccttg tcgatgcgcc gatagaaaca gctgcgccgg
     1561 ccggtgtggc aggccgcgcc gcggcccatc tgccgggcct tcacctgcag cacgtcctga
     1621 tcgcagtcgg taaggacttc cgtgacctgg agcagttcgc cggaggtttc gcccttcttc
     1681 cagagtgcct ggcgcgagcg gctccagaaa tgagcatatc ccgtgtccag tgtcaggcga
     1741 agcgcctccg cattcatgtg ggcgaacatc agcacggtac catcgctcga atcggtgacg
     1801 atggccggta tgaggccttc ggaatcgtac ctcggctgaa acgtctccgt ctcgtcgagg
     1861 ctgtccgcca tcagagcgaa aagccgcccg aaggcgcgcg aaccagttcc atgaagcgcg
     1921 cctgcagtac ggggttcgac ttgaactcgc cggtaaactg ggtggtgatg gtcgccacgt
     1981 tgcgcttgcc gatgccccgc atcgacatgc aggtatggac cgcctcgatg agcaccgcga
     2041 cgccgcgcgg ctgaagcgcg ttcatgatgg cattggcgat ctgggcggtc atggtctcct
     2101 gggtctgcag gcggcgggca tagatttcca ccacacgggc cagcttggag attcccacca
     2161 ccgacttggt gggaaggtaa gcgacatggg cgcgcccgac gaagggagcg atatggtgtt
     2221 cgcagtgcga gttgaactcg atgtcgcgca gcatgacgat gtcgtcgtag ccccccacat
     2281 cgtcgaaggt gcgcgacagg gcttcggccg gatcatcttc gtagccgctg aaatactcgc
     2341 ggaaggcctt ggccactcgc ttcggggtat cgagcaggcc ttcgcggtcc gggtcgtccc
     2401 cggcccaggc cagcaggacc cggacggcgg cctcggcttc ctcctggctg gggcgcttga
     2461 ccggcttgag cgggatcttc ttgatgatgg cgtccattgg tcctgacttt actcgcaaac
     2521 cgtgtctcga taacagcttc cgcaggactg ccgcccggcg aaggcatggc tccggccttg
     2581 cggccgacct tccggggccc ccatatcatg gctggtccct tcagggcaaa ccggcaaacg
     2641 ccgtgcttgc cggagatcgc ggaaggatta ggatatcggg atgatcgacg acatctacaa
     2701 ccggaagatt ctggggttcg cggccgatat tccgcggttg cagaggctgg ccgcgccgga
     2761 cgcaacggcc gtcgcccatt cccgcctctg cggctcgaaa gtgacggtgg acctgaagat
     2821 gaaggatggt gtcgtcacgg acttcgggca tgaggtgaag gcctgtgcgc ttggccaggc
     2881 ctcgtcctcg atcatggccc gccatgtggt cgggtcgact gccgtcgagc tccgcgatct
     2941 gcgggaccag atgcaccgga tgctgaagga ggagggcccc ccaccgggtg gcaagtggtc
     3001 cgatctggag gccctggtgc cggtgcggaa cttcaaggca cgccatgcct cgacccttct
     3061 caccttcgac gccgtcgctg acgcagtagg gcagatcgag gcgaagggca gggagctggc
     3121 ccatggctga cgacagaccg gccgccctcc gcgccgcaga gcttcccgcc cggactgcct
     3181 cgatctatcc ggcggccttc gccaaggctc tggaggggcg gagcaagcgc gctctgggcg
     3241 atgccttcga cctgacccag ttcggtgtca attacacggt cttggagccg ggttcggcat
     3301 cctccgagcg ccactggcac gcggtggaag acgaactcgt ctttgtgctc gagggcgaag
     3361 tcacgctgat cgacgaggcc ggcgagcatc ttctggtgcc gggcatgtgc gccggcttca
     3421 aggcgggtgt tgccaatggc cacaagctgg tcaaccgcgg tgccgttcgg gcggttttcc
     3481 tcgaaatcgg gacgcggtcc ccgaccgaaa gcgtggtcta tccggatgtc gacatgctgg
     3541 cccggaaggc cggcggcaag tacatcctta cgcacaaaga cggcacgccc tattgaagac
     3601 gatcctgctg gcactgatcc gggcttatca gctgaccctc tcggccttga tcgggcgacg
     3661 ctgccgctat ctgccgacct gttccgacta tgccagcgag gcgatccggc tgcacggtgc
     3721 ctggcacgga accgtgcttg gatttgcccg ggtctgccgc tgccatccct ggggccagtc
     3781 gggcttcgat ccggtgccga aggtctatga cggaccggta gggcgcatga agacaggggc
     3841 cccggagggc ccctgaagtg tctgcgctga tcctggggac gtgttacagg ccgaagcggt
     3901 aggcgaaacc gaggcgaacc gtgtg
//
LOCUS       DBNGVR010000480         3187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2538259, whole genome shotgun sequence.
ACCESSION   DBNGVR010000480 DBNGVR010000000
VERSION     DBNGVR010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2538259"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="AC16A0_10140"
     CDS             <1..436
                     /locus_tag="AC16A0_10140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3105738.1"
                     /translation="LMADRRAVSQILINLLSNAIKFTPAGGLIDVKADRDSRGGLFIS
                     IKDNGPGIPPHEIEAAMSAFSRGSMATKKAIDGAGLGLPIVKGLMSVHGGTLELRSKA
                     GEGTEAIVTFPPGRVLDGPRGEVLASPGISTESQRKLIAITG"
     gene            531..2693
                     /locus_tag="AC16A0_10145"
     CDS             531..2693
                     /locus_tag="AC16A0_10145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3105739.1"
                     /translation="MPSKRSFLSDFAARVLVPSILAAGGIATLLLYIVNGIFDETNRL
                     DAGYAKQSASSAIHSSLENLESLIHDNAVWDDAVANAYGDINSEWLNGTWGDATALGI
                     YDHVNLVERSGRTLYAARNGDQTEDSAENIYGTEILELLAPMPADETLFKVTSGIVRK
                     DNKLYVVAAAPILPTSEDVEIPNGKARYLIFGKTVDNKLLAELSRRFVLKNLHVSSSL
                     INAPDSISILSPSGVRIGTLAWEERSPGDILKSKYSSVIRIMLALFLFVIAVLIYTSW
                     RGFRDAHVSQTAAVAKSLRDDLTGLANRRELLSVLTDRLHEARIGRTGLSLVYADLDG
                     FKEVNDSYGHEIGDLLLKAVAAGFAHLAQDMGLVARLGGDEFAIIVTGEDSTARSREL
                     ANNMISFLSEPMIFGGRVASVSVSVGIVDLEKDDADVEEIIRRADVAMYAAKSAGRNR
                     LHIYDKTLDYKRDENRSIARALREHIDMGRLTVVYQPIVDSRSRRIRGVEALVRWPRD
                     DRIHYTPDVFIPVAEESGLIEDLGYFVLSEACKQAAQWPDIFVSVNVSPVQFMNPGFA
                     ELVERTLTRTGLQATRLELEVTEGFIIDNADRANVIINRLHDMKVGVALDDFGTGFSS
                     IGHLRRFKFDKLKLDRSMVTDILRQPSALRLVQGTIAMADALGLMVVAEGVEDENQVS
                     VLRLAGCSQFQGFLFSRPVSAQEIGSMLTAAAPLAASA"
     gene            complement(2690..>3187)
                     /locus_tag="AC16A0_10150"
     CDS             complement(2690..>3187)
                     /locus_tag="AC16A0_10150"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR3105740.1"
                     /translation="AHGLYGGAAEDWVGPGTGVDAAGLARKADAVRRAVALHRDHLGD
                     PLEVLRRLGGRELAAMAGAILAARLNRVPVLLDGYVATAAAAVLHAMDPAALDHCLAG
                     HRSAEPAHRRLLERIGKEPVLDLGMRLGEGSGAALAAAMVKAAIALHTGMATFESAGV
                     SDRQD"
BASE COUNT          593 a         1058 c          963 g          573 t
ORIGIN      
        1 gctcatggcc gaccgccgtg ccgtcagcca gatcctgatc aatctcctgt cgaacgcgat
       61 caagttcact cccgctggag gccttatcga tgtgaaggcc gaccgcgaca gccgcggcgg
      121 cctcttcatt tcgatcaagg acaacggacc gggcattccg ccccacgaga tcgaggcggc
      181 aatgagtgcc ttctctcgtg gcagcatggc gaccaagaag gcgatcgacg gcgccggcct
      241 cggacttcca atcgtgaagg gcctcatgtc ggtccatggc ggcacgctgg aattgcgcag
      301 caaagcgggc gaaggtaccg aggcgatcgt caccttcccg ccaggacggg tcctcgatgg
      361 accacgtgga gaggttctag caagtccagg tatttcaacc gagagtcagc ggaagctgat
      421 cgcaataacc ggttaactct cgtgtgattt ccgcgctttg ggctaggccg ccgatagacg
      481 gcgttaagca ggatcctgct acgataacac cctaagttgc aggatccgcg atgccctcca
      541 aacggtcgtt cctctccgac tttgccgcgc gagtactggt gcccagcatc ctcgccgcag
      601 gcggcatcgc gacgctcctg ctttacatcg tcaatggaat cttcgacgag accaaccggc
      661 ttgacgccgg ctatgccaag cagtcggcca gttccgcgat ccactcatcg ctcgagaacc
      721 tcgagagtct gatccacgac aatgcggtct gggatgatgc ggtcgcaaac gcctatggcg
      781 acatcaactc ggaatggctg aacggcacct ggggtgatgc gaccgccctc ggtatctacg
      841 atcacgtgaa tctcgtggaa cgctccggca ggacactcta cgcggcgcgc aacggagatc
      901 agacagagga ctcggccgag aacatctacg gcaccgaaat cctcgagctg cttgccccaa
      961 tgcccgcaga cgaaaccttg ttcaaggtta catcgggcat cgtacgcaag gacaataaac
     1021 tctatgtggt ggcggccgct cccatcctgc ccacctccga agacgtcgag atccccaacg
     1081 gcaaggcccg ctatctcatt ttcgggaaga cggtggacaa caagctcctg gccgagctct
     1141 cgcgccgctt cgtgctgaag aaccttcatg tctcgtcgtc gctgatcaac gcacccgatt
     1201 cgatctccat cctctcgcct tcaggtgttc gcatcggaac cctggcctgg gaggaacgca
     1261 gccccggcga catcctgaag tcgaagtaca gctctgtcat ccggatcatg ctggcgctgt
     1321 tcctcttcgt catcgcggta ttgatctaca ccagctggcg cggcttccgt gacgcgcatg
     1381 tgagtcagac cgctgcggtg gccaagtcgc tgcgtgacga tctcactggg cttgccaacc
     1441 gccgtgagct cctgtcggtt ctgaccgacc ggctccacga ggcgcgcatc ggccggaccg
     1501 gcctgtccct ggtctacgcc gacctcgatg gtttcaagga ggtcaatgac tcctacggcc
     1561 acgagatcgg cgatctcctg ctcaaggcgg tggcggcggg ctttgcccac ctggctcagg
     1621 acatgggact ggtggcacgg cttggcggcg acgagttcgc catcatcgtc acaggcgaag
     1681 acagcacggc aagatcgcgc gaactcgcca acaacatgat ctccttcctg tcggagccga
     1741 tgatcttcgg cggccgggtg gcctcggtat cggtcagcgt tggcatcgtc gacctggaga
     1801 aggacgacgc ggacgtcgag gaaatcatcc gccgcgccga cgtggccatg tatgccgcga
     1861 aatcggctgg ccgaaaccgc ctgcacatct atgacaagac attggactac aagcgggacg
     1921 agaaccgctc catcgcccgt gccttgcggg agcacatcga catgggccgc ctcaccgtgg
     1981 tgtatcagcc catcgtcgat tcccgcagcc gccgcatccg gggcgtcgag gcgctggtgc
     2041 gctggccccg ggatgaccgc atccactata cgcccgacgt cttcattccg gtcgcggagg
     2101 agtcggggct gatcgaagac ctcggctatt tcgtcctgtc cgaggcctgc aagcaggcag
     2161 cgcaatggcc ggacatcttc gtgtcggtca atgtctcgcc ggtgcaattc atgaatcccg
     2221 gcttcgccga actggttgaa cggacgctca cgcgcaccgg tcttcaggcc acccgcctgg
     2281 agctggaggt caccgagggc ttcattatcg acaatgccga ccgcgccaat gtcatcatca
     2341 accgcctgca tgacatgaag gtcggtgtgg cgctcgacga cttcggcact ggattctcca
     2401 gcatcggcca tctgcgccgc ttcaagttcg acaagctgaa gctggaccgc tcgatggtca
     2461 ccgacattct ccgccagccc tcggcgctgc ggctggtgca gggcacgatc gccatggccg
     2521 acgccctggg cctcatggtg gtggccgaag gcgtcgaaga cgagaaccaa gtgtcggtgc
     2581 tgcgtctggc cggctgcagt cagttccagg gcttcctgtt ctcccgcccg gtctccgcgc
     2641 aggagattgg cagcatgctg acggccgcgg caccgctggc cgcttcggcc tagtcctggc
     2701 ggtcgctgac cccggcgctc tcgaatgtgg ccatgcccgt atgcaacgca attgcggcct
     2761 tgaccatggc ggcagcgagc gcagcgcccg acccttcgcc aagccgcatg cccagatcca
     2821 gcacgggctc cttgccgatc ctttccagaa gccggcgatg cgccggctcg gccgagcggt
     2881 ggccggcgag gcagtgatcc agtgcggcag gatccatggc atgcagaacc gcagctgccg
     2941 cagtcgcaac gtaaccgtcg agcagaacgg gaacccgatt gagacgggcg gcgagaatgg
     3001 cgccggccat ggcagcgagc tctcgcccgc caagccgccg caggacttcc agcggatctc
     3061 cgagatgatc gcgatgcagt gccacggcgc ggcgcaccgc gtcggccttg cgggcaagcc
     3121 ccgccgcgtc cacgccggtt ccgggtccga cccagtcctc cgccgcccca ccatagagcc
     3181 catgcgc
//
LOCUS       DBNGVR010000481         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5092861, whole genome shotgun sequence.
ACCESSION   DBNGVR010000481 DBNGVR010000000
VERSION     DBNGVR010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5092861"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1195
                     /locus_tag="AC16A0_10155"
     CDS             152..1195
                     /locus_tag="AC16A0_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3105741.1"
                     /translation="MTRPVHRLLDGTRALESGQLDRPLAVTSRDEIGDLTTAFNHMVE
                     QLRLKERIRETFGKFVDPRIVASMISDDAANAERVERRIVTVFFSDIAGFTSISEELA
                     PGAIVNLLNNYFSAVTAPIRASNGIVDKYMGDGVMAFWSPPFSEGDSHAASACRAALA
                     QQDAVEQLNKELPNILGLRRGTPVLSVRMGLATGEAIVGTMGSATSKTFTVMGDTVNL
                     ASRLEGANKIYGTKIIVTAETVALASNEIAFRELDLITVAGRTEPVHIYEVLGTKDAL
                     DPRDSSLRDAFARGLEAYRAQDWAQAERQFQLCLELNPKDGPARTFAGRIAQFRHEPP
                     PPGWDGVWRLADK"
     gene            complement(1256..2143)
                     /locus_tag="AC16A0_10160"
     CDS             complement(1256..2143)
                     /locus_tag="AC16A0_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3105742.1"
                     /translation="MRHAIGIDVGGTKISAAIVELDTGRLLHRREVATHAARGGEAVL
                     DTVRGLLIDLGRTADESRLRIDAVGLGICEIVGLNGRLQSASLIDWRDLNPISSPHGL
                     PPVHLVSDVRAAALAEARFGAARQVRDALYVSVGTGIASVLLIEGLPYAGAHGAALVL
                     ATAAPVPIGSGQAAMLEEIASGPALARQFGDSGGDARIVIAAAIRGDQRAHAIVMQAT
                     SLLGAAIAQLANCLDPSVIVIGGGLGSAPGPYFESLKEGITDRIWKGLDRQIDVRRAV
                     LGADAGLAGAALAADLRLR"
     gene            complement(2148..>2411)
                     /locus_tag="AC16A0_10165"
     CDS             complement(2148..>2411)
                     /locus_tag="AC16A0_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3105743.1"
                     /translation="AGKATIAPGGRSAVIEHGGDAGIYVEAGRLNVQLVDSEQAQTWF
                     ELAPGDGFYLPEGARYRLFNMGTQSCDALFGVAPSYLPRGTAP"
BASE COUNT          439 a          818 c          756 g          398 t
ORIGIN      
        1 tggaagagaa ccgcaggtcc atgcggcagc tcctcgagaa tgccgtctcc gagaccgaaa
       61 gtgaggccga gcgggccatg atgatcgcct tgggctcacc gttctcgcca cggcgcttgg
      121 cctcatcttt gcctatctgg tcagcagtgg cgtgacacgc ccggttcatc ggctcttgga
      181 tggcacgcgt gcgttggaat cgggacagct cgaccggccg cttgccgtga cgtcgcgtga
      241 cgagatcggg gatctcacga cggcgttcaa tcacatggtc gagcaactgc gcctcaagga
      301 gcggattcgc gagaccttcg ggaagttcgt cgacccccgc atcgtcgcca gcatgatctc
      361 ggatgacgcg gcaaacgcgg agcgtgtcga acgccggatc gtgacggtct tcttctcgga
      421 tatcgccggc ttcacgtcga tcagcgagga gctggcgcct ggcgccatcg tcaatctcct
      481 gaacaactat ttctcggccg tcaccgcgcc gatccgcgct agcaacggca ttgtcgacaa
      541 gtacatgggc gatggcgtga tggctttctg gtcgccgccc ttttcggagg gcgacagcca
      601 tgccgcctcc gcctgtcgtg ccgcgctcgc gcagcaggac gctgtcgaac agctgaacaa
      661 ggagttgccc aacatcctcg ggcttcgccg cggcacgccc gtcctgtcgg tgcgaatggg
      721 cctcgcgacg ggcgaggcga ttgtcggcac gatgggttcg gcaacctcca agaccttcac
      781 cgtcatgggc gacacggtca atcttgcttc ccgcctcgaa ggcgcaaaca agatctacgg
      841 cacgaagatc atcgtgacgg ccgagactgt cgctctggcg tcgaacgaga ttgccttccg
      901 cgagctcgat ctcatcacgg tggcaggaag gacagaaccg gtacacatct acgaggtgct
      961 tgggaccaag gatgcgctcg acccgcgcga ctcaagcctc cgcgacgcct tcgcccgtgg
     1021 cctcgaggcc taccgggcgc aggactgggc ccaggccgag cggcagttcc agctatgcct
     1081 ggaactcaac cccaaggacg ggcctgcccg gacatttgcc gggcgcattg cccagttccg
     1141 gcacgagccg ccaccgccgg ggtgggacgg tgtctggcgg cttgcggaca aatagaacag
     1201 agacgtgacc ggcgccgctg cggcgtgatc ttgccggatg agcacatcct gaggtctatc
     1261 gcagacgcag gtcagctgcc agcgccgcgc cagccagccc cgcatcggcg ccaagcacgg
     1321 cgcgcctgac gtcgatctgc cggtccagcc ccttccagat gcgatccgtg atcccttcct
     1381 tgagggactc gaaatagggg cccggtgcac tcccgagtcc gccgccaatg acgatcaccg
     1441 acggatcgag acaattggcc agctgggcaa tcgccgcgcc cagcagcgac gtcgcctgca
     1501 tcacgatggc atgcgccctc tgatcgccgc ggatcgctgc agcaatcacg atcctcgcat
     1561 cgccgccgga gtcaccgaac tgccgtgcta gggccggccc ggacgcaatc tcctccagca
     1621 tcgcggcctg gccactaccg atcggcaccg gtgcagccgt cgccagcaca agggccgcgc
     1681 catgcgctcc ggcatagggc aggccctcga tcagcagcac cgatgcgatg ccggtcccga
     1741 ccgagacata gagagcgtcg cggacttggc gtgccgcgcc gaagcgggcc tccgcgagtg
     1801 ctgccgcccg cacgtccgac accaaatgaa ccggcggaag gccatgcggc gaggaaattg
     1861 ggttcaggtc gcgccagtcg atgagcgagg cgctttgcaa ccgcccattc agtccgacaa
     1921 tctcgcaaat gccaagtcca acggcgtcga tgcgcaggcg ggactcatcc gcagtccggc
     1981 caagatcgat caagaggcca cgaaccgtat cgaggaccgc ttcaccgccg cgcgccgcat
     2041 gtgtcgctac ctcacgccgg tgcagtagcc gcccggtgtc gagctcgacg atcgcggcgg
     2101 atatcttggt tccaccgaca tcgatgccga tcgcatggcg catctcctta cggtgccgtt
     2161 ccgcgcggca gataggaggg tgccaccccg aacaaggcat cgcaggactg cgtgcccatg
     2221 ttgaaaagac gatagcgagc gccctccggc aggtagaagc cgtcgccagg cgcgagttcg
     2281 aaccatgtct gggcctgctc gctgtcgacg agctgaacat tgagacgccc ggcctccaca
     2341 tagatgcctg cgtcgccgcc atgttcgatt accgcactgc gtccgcccgg cgcgatggtc
     2401 gccttgccgg c
//
LOCUS       DBNGVR010000482         2284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2735129, whole genome shotgun sequence.
ACCESSION   DBNGVR010000482 DBNGVR010000000
VERSION     DBNGVR010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2735129"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC16A0_10170"
     CDS             <1..573
                     /locus_tag="AC16A0_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR3105744.1"
                     /translation="MAEAAVAIAAARLTALDALRFHIGETRETSRFPWSEISLDGEIE
                     ALVTAMPAVRAEDEYRRLLNDSRRQDRGAGRTLSGPHRSDLAVRHGPKSMPAGLCSTG
                     EQKALLIGLILAQARAVAGVSGIPPLLLLDEVAAHLDRERRQGLFGALAALGCQAWMT
                     GTDVELFADLGREGMVFHVEDGTVSDVKRV"
     gene            573..>2284
                     /locus_tag="AC16A0_10175"
     CDS             573..>2284
                     /locus_tag="AC16A0_10175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR3105745.1"
                     /translation="MTEEEKQNGVAAYGAESIKVLKGLDAVRKRPGMYIGDTDDGSGL
                     HHMVYEVVDNAIDEALAGHADRVTVTLNADGSCTVTDNGRGIPTDIKDDDDHEPKRSA
                     AEIVMTELHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLKLVIFRNGKEHVIAFTHG
                     VADGPLQIVGAAPGRRGTEVTFLPSTETFSKTEFDFATLEHRLRELAFLNSGVRILLA
                     DRRGVEPKEIELFYEGGIAAFVRYLDRTKVAVLPEPIMIHGEKDGITVDCALWWNDSY
                     HENVLCFTNNIPQRDGGTHLAGFRAALTRVVNNSANESGIAKKEKVALTGDDAREALT
                     CVLSVKVPDPKFSSQTKDKLVSSEVRPVVEGLVNDQLGAWFEEHPGEARSIVGKVVEA
                     AAAREAARKARELTRRKGALDIAGLPGKLAECQNRDPAQSEIFIVEGDSAGGSAKQAR
                     NRENQAVLPLRGKILNVERARFDKMLSSAEIGTLITALGTGIGREEFNIEKLRYHKII
                     IMTDADVDGAHIRTLLLTFFDRQMPEVIERGHLYIAQPPLYKVKRGSSEQYLKDAKAL
                     ENYL"
BASE COUNT          434 a          726 c          757 g          367 t
ORIGIN      
        1 atggcggaag ccgcggtggc catcgcggcg gcccggctga cggcgctgga cgcgctccgc
       61 ttccacatcg gtgaaacccg ggagacaagc cgctttccct ggagcgaaat cagcctggac
      121 ggggagatcg aagcccttgt tacggccatg ccggcagtgc gcgccgagga cgaatatcgt
      181 agacttctga acgattcgcg cagacaagac aggggtgccg ggcgtacact gagcggaccg
      241 catcgcagcg atctggccgt ccgtcacgga cccaagtcca tgcccgccgg actgtgttcg
      301 accggagagc agaaggctct cctgatcggg ctgatcctgg cccaggcgcg ggcggtcgcg
      361 ggcgtcagcg gcatcccgcc gctgctgctt ctggacgagg tggcggcgca tctggaccgg
      421 gagcggcggc aaggcctgtt cggggccctg gcggcgctgg ggtgccaggc ctggatgacg
      481 gggacggacg tcgagttgtt cgcggatctt ggccgcgaag gtatggtctt ccatgtggag
      541 gacggaactg tttcggacgt gaagagagtc tgatgaccga agaagagaag cagaatggcg
      601 tggcggccta cggggccgag tcgatcaagg tgctgaaggg cctggatgcc gtgcgcaagc
      661 ggcccggcat gtacatcggc gacaccgatg acgggtccgg ccttcatcac atggtctacg
      721 aggtggtcga caatgcgatc gacgaggcgc ttgccggcca tgccgaccgg gtcacggtca
      781 ccctgaacgc cgatggctcc tgcacggtga ccgacaatgg ccgcggcatt ccgaccgaca
      841 tcaaggacga tgacgatcac gagcccaagc gctcggcagc cgagatcgtg atgacggaac
      901 tgcatgccgg cggcaagttc gaccagaact cctacaaggt ttcgggcggt ctccacggcg
      961 tgggcgtgtc ggtggtcaat gccctgtctg tctggctgaa gctggtcatc ttccgcaatg
     1021 gcaaggagca tgtgatcgcc ttcacccatg gcgttgccga tggcccgctg cagatcgtcg
     1081 gcgcggcacc gggacggcgc ggcaccgagg tgacgttcct gccctcgacc gagaccttct
     1141 ccaagaccga gttcgacttt gccaccctcg aacatcgcct gcgcgagctg gctttcctca
     1201 attccggcgt ccgcatcctg ctggccgacc gccgcggggt tgagccaaag gagatcgagc
     1261 tcttctatga aggcggaatc gccgccttcg tgcgctatct cgaccgcacc aaggtggccg
     1321 tgctgcccga gccgatcatg atccacggcg agaaggacgg catcacggtc gactgcgcct
     1381 tgtggtggaa cgacagctat catgagaatg tcctgtgctt caccaacaac attccgcaac
     1441 gcgacggcgg cacgcatctg gcgggtttcc gcgcggcact gacgcgcgtg gtcaacaact
     1501 ctgccaacga aagcggaatc gccaagaagg agaaggtggc actcaccggc gacgacgcgc
     1561 gcgaggcgct cacctgcgtg ctctccgtga aggttcccga tccgaagttc tcgtcgcaga
     1621 ccaaggacaa gctcgtctcg tcagaagtgc gcccagtggt cgagggcctg gtcaacgacc
     1681 agctgggcgc ctggttcgag gaacatcccg gcgaggccag gtccatcgtc ggcaaggtcg
     1741 ttgaagcggc ggccgcgcgc gaagccgcgc gcaaggcgcg cgaactcacg cggcgcaagg
     1801 gcgcgctcga catcgccggc ctgcccggca agctggccga atgtcagaac cgcgacccgg
     1861 cgcaatcgga aatcttcatc gtcgagggcg actcggccgg cggttccgcc aagcaggcgc
     1921 gcaaccgcga gaaccaggcc gtgctgcccc tgcgcggcaa gatcctcaat gtcgagcgcg
     1981 cgcgcttcga caagatgctg tcctccgccg agatcggcac gctgatcacc gcgctgggca
     2041 ccggcatcgg ccgcgaggag ttcaacattg aaaagctgcg ctaccacaag atcatcatca
     2101 tgaccgacgc cgacgtggac ggcgcgcata tccgcaccct gctgctcacc ttcttcgacc
     2161 ggcagatgcc ggaagtgatc gagcgcggcc atctctatat tgcccagccg ccgctctaca
     2221 aggtgaagcg cggctcgtcc gaacaatatc tcaaggacgc caaggcgctg gagaactacc
     2281 tgat
//
LOCUS       DBNGVR010000483         2089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4110476, whole genome shotgun sequence.
ACCESSION   DBNGVR010000483 DBNGVR010000000
VERSION     DBNGVR010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4110476"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1189)
                     /locus_tag="AC16A0_10180"
     CDS             complement(8..1189)
                     /locus_tag="AC16A0_10180"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004116324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIR3105746.1"
                     /translation="MSTVTDISAELDAIRAQSLWKTERPILGHQSSHIAVTSGREVLN
                     FCANNYLGLADHPALVAAAKEALDSHGLGMASVRFICGTSDLHLALERRIAGYLGMDD
                     AILFAACFDANGAVFEPLFGEQDAIVSDALNHASIIDGIRLSKAKRFRFANGDMGDLE
                     TQLKAARAAGARRVIIVTDGVFSMDGYFARLPEIRKLADAYEALIMVDDCHATGFIGP
                     QGRGTPARAGVKVDILTGTFGKALSGSAGGYIAASQPIVDLMRQRARPYLFSNALPPP
                     IVAASLKAIDLAEAGDDLRTRLFDNARRFRTGMAAAGFNLLPGEHPIIPVMLGEAKLA
                     QDMASRLYELGVYVTGFFFPVVPKGQARIRTQMSAAHTPADVDGAISAFTSSARDLGV
                     I"
     gene            1289..>2089
                     /gene="trmFO"
                     /locus_tag="AC16A0_10185"
     CDS             1289..>2089
                     /gene="trmFO"
                     /locus_tag="AC16A0_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR3105747.1"
                     /translation="MKPIHIIGGGMAGSEAAWQAACAGIPVVLHEMRPAVGTDAHKTE
                     GLAELVCSNSFRSDDHMQNAVGLLHEEMRRAGSLIMAAAGRHKVPAGGALAVDRDGFS
                     DDVTDTLMRHPLVTVERGEIAGLPSEDWDNVIVATGPLTSPALAGAIRALTGEDQLAF
                     FDAIAPIVHRESIDMDIAWFQSRYDKVGPGGTGKDYINCPMDKAQYETFVQALIEGGK
                     TEFKQWEGTPYFDGCLPIEVMAERGAETLRHGPMKPRGLTNAHDPQVKP"
BASE COUNT          374 a          683 c          700 g          332 t
ORIGIN      
        1 gcaacattca gatgacaccg agatcgcggg ccgagcttgt gaaggcagaa atcgctccgt
       61 caacatctgc tggagtgtgc gccgcggaca tctgggtccg gatgcgggcc tgccctttcg
      121 gcacgacggg gaagaagaag ccggtcacat agacacccag ttcatagagc cgggaggcca
      181 tgtcctgggc cagtttcgct tcgcccagca tcaccgggat gatgggatgt tcgccaggca
      241 gcaagttgaa gcccgctgcc gccatgccag tgcggaagcg ccgcgcattg tcgaagagcc
      301 gcgtgcgaag atcgtccccg gcttcggcga ggtcgatggc cttcaggctt gccgccacga
      361 tgggcggcgg cagggcgttt gaaaagagat agggccgcgc ccgctgccgc atcaggtcca
      421 cgatgggctg cgacgccgcg atatagccgc ccgccgagcc gctcagcgcc ttgccgaatg
      481 tccctgtcag gatatcgacc ttgacacctg cacgggccgg cgtgcccctg ccctgcggcc
      541 cgatgaagcc ggtcgcatgg cagtcatcca ccatgatgag cgcctcgtag gcgtcggcga
      601 gcttccggat ctccggcagc ctggcaaagt agccgtccat cgagaagacc ccgtcggtca
      661 cgatgatcac gcggcgggca ccggccgcgc gcgcggcctt caactgggtc tcgaggtcgc
      721 ccatgtcccc attcgcgaaa cggaaccgct tcgccttcga caggcggatg ccgtcgatga
      781 tcgaggcatg attcagcgca tcggatacga tcgcgtcctg ttcgccgaag agcggctcga
      841 agaccgcccc attggcatcg aaacaggcgg cgaaaaggat ggcgtcgtcc atgccgaggt
      901 agcccgcgat gcgccgctcg agcgcgagat gcagatccga ggttccgcag atgaagcgga
      961 ccgaggccat gcccagcccg tggctgtcga gggcctcctt ggcggcggcg accagcgcgg
     1021 gatggtcggc caggcccaga tagttgttgg cgcagaagtt caacacctcg cgtccgctgg
     1081 tgacggcaat gtgcgaggac tggtgcccca ggatcggacg ctccgttttc cacagggact
     1141 gggcacggat ggcgtcgagt tcggcggaga tatcggtaac ggtgctcatg cgccggaccc
     1201 tagcctgccc gtgcccgctc cgaaagcgtc gaagtcgctc acccgttcgg ctttgaagcg
     1261 ggctccgccc catgctaacg gggaggccat gaagccaatc cacatcatcg gcggcggcat
     1321 ggccggatcg gaggctgcct ggcaggccgc ctgcgccggc atcccggtcg tgctgcacga
     1381 aatgcgcccg gccgttggca ccgacgcgca caagacggag ggcctggccg agctggtctg
     1441 ctcgaactcc ttccgctcgg atgaccacat gcagaacgcc gtcggactcc tgcacgagga
     1501 gatgcgacgc gccggctcgc tcatcatggc cgccgccggc cgtcataagg ttcctgccgg
     1561 cggcgctttg gcggttgatc gcgacggctt ctccgatgat gtcactgaca ccctgatgcg
     1621 ccatccgctg gtaacggtgg agcgcggcga gatcgcgggg ctgccatccg aggactggga
     1681 caatgtcatc gtcgccacgg ggccgctgac gtcgcctgcg ctcgcagggg ccatccgcgc
     1741 cttgaccggc gaggaccagc ttgccttctt cgacgccatt gcgcccatcg tccaccgcga
     1801 gagcatcgac atggatatcg cctggttcca gtcgcgctac gacaaggtgg gacccggcgg
     1861 caccggcaag gactacatca actgcccgat ggacaaggcg cagtacgaga ccttcgtgca
     1921 ggcgctgatc gaaggcggca agaccgagtt caagcagtgg gagggcacgc catatttcga
     1981 cggctgcctg cccatcgagg tgatggcgga gcgcggggcg gaaacgctgc gccacgggcc
     2041 catgaagccg cgcggcctca ccaatgcgca tgacccgcag gtgaagccc
//
LOCUS       DBNGVR010000484         2159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4699609, whole genome shotgun sequence.
ACCESSION   DBNGVR010000484 DBNGVR010000000
VERSION     DBNGVR010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4699609"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1415)
                     /locus_tag="AC16A0_10190"
     CDS             complement(168..1415)
                     /locus_tag="AC16A0_10190"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019478 - D-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HIR3105748.1"
                     /translation="MKALVLGAGIVGITTAYELNRDGHEVTVIDREAEPAAFTSYSNA
                     GLFAPGHAYAWSSPAAPGILLRSLWRGDQALRLKLSADPRFWSWMWRFWRQCTPERAA
                     LNTTRKVRLCNYSLAVFHETLARTGVAYDGRKGGLLYLYSSEAALKAASAKSRILSDN
                     GCRIETLDRDGVAAKDPALGPVKESFAGALYAPDDESGDCRLFARNMGDWLKGQGVAF
                     KFGTAITGFETAGDRVAAVLTDKGRETADVIVLCLGVYSPQLARQIGHDLPIYPVKGY
                     AMTAPVAGRNNPPTIGGVDEETLVAYAPYGDRIRVTATAEFSGYDRSHRPQDFATMLA
                     TMRRLFPDGADWDQASTWAGLRPMTPEGTPILGPSRMKNLWFNTGQGHMGWTMSHGAA
                     RIKADLLAGKRPPIPLDGMTAVT"
     gene            1464..>2159
                     /locus_tag="AC16A0_10195"
     CDS             1464..>2159
                     /locus_tag="AC16A0_10195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3105749.1"
                     /translation="MPSTTHPHAFHFERAVDSYWEASADPLGIATPALTAHESCDVAI
                     IGAGFTGLSAAIELAGQGLDVRVLEAGRVGWGASGRNGGFACIGSHKLSYTEMIRTYG
                     LTATQHFHAAMAESVDLVRDNCRRFGIDAWIHGAGEVTLAHLPNRVAELRGDQAFLKT
                     TFGEETELLSVADLKAQGLWGPTFHGGLRGKIGFGIHPLNYVRGLARAAAAAGAKIHA
                     SSRLTRWEETAAGH"
BASE COUNT          338 a          712 c          751 g          358 t
ORIGIN      
        1 tgagttgccg gctggcccgg ctgcgggcac gtggcgccgc aggaccggag gatgccggat
       61 tgcttttgtt gttcgcggtg aacagccatt ctctgaccat caggtcaaaa ttgctgcacc
      121 cgcgaagagg ctgtgaaggg ttaatggggc cagaatgtgt ccccgattta cgttacggca
      181 gtcatgccat cgagcgggat cgggggccgt ttccctgcga gcagatccgc cttgatgcgg
      241 gccgcgccat gactcatcgt ccagcccatg tggccctggc cggtgttgaa ccagagattc
      301 ttcatccgcg aggggccgag gatgggggtt ccttccggcg tcatgggacg aagcccggcc
      361 caggtcgagg cctggtccca gtccgcgccg tccggaaaga ggcgccgcat ggtggccagc
      421 atggtggcga agtcctgcgg gcggtggctg cggtcgtagc ctgagaattc cgccgtcgcg
      481 gtgacgcgga tgcggtcgcc gtacggggca taggccacca gcgtctcctc atccaccccg
      541 ccgatggtgg gcgggttgtt gcgccccgcg accggcgccg tcatggcata gcccttgacc
      601 gggtagatcg gcaggtcgtg cccgatctgg cgggccagct gcggggaata gacgccgagg
      661 cagagcacga tcacgtccgc cgtctcacgg cccttgtcgg tgagcacggc ggccacccgg
      721 tcgcccgccg tctcgaaacc ggtgatggcg gtgccgaact tgaaggccac gccctgcccc
      781 ttgagccagt cgcccatgtt gcgggcgaag agccggcaat cgccgctctc gtcgtcgggc
      841 gcatagaggg cgccggcgaa actttccttc acgggcccga gcgccggatc cttcgccgcc
      901 acgccgtcgc ggtccagcgt ctcgatgcgg cagccattgt cgctgagaat gcgcgacttg
      961 gcggaggccg ccttcagcgc cgcctccgag ctgtagagat agagcagccc accctttcgg
     1021 ccatcatagg caacgccggt gcgggccagg gtctcgtgga agacggcgag cgaataattg
     1081 cacagcctca ccttgcgcgt ggtgttgagt gccgcgcgct cgggcgtgca ttgccgccag
     1141 aagcgccaca tccacgacca gaagcgcgga tcggcggaga gcttgaggcg cagcgcttgg
     1201 tcgccgcgcc atagcgagcg cagcaggatt ccgggtgctg cgggcgagga ccaggcatag
     1261 gcatgacccg gcgcgaagag ccccgcattg gagtagctgg tgaaggcggc gggctcggcc
     1321 tcgcggtcga tgacggtgac ctcgtggccg tcgcggttca gctcgtaggc cgtggtgatg
     1381 ccgacgatcc ccgcgcccag taccagtgcc ttcatcgcga cctctttgcc attccccgga
     1441 gcggatgcta gcaagcctgc atcatgccat ccaccacaca tccgcatgcc ttccatttcg
     1501 aacgcgccgt tgacagttac tgggaggcct ccgccgaccc gctggggatc gcgacgccgg
     1561 cgctgacggc ccacgagtcc tgcgacgtcg ccatcatcgg cgcgggcttc accggtctct
     1621 cggcggcgat cgaactggcg ggacagggcc tcgacgtgcg cgtcctggag gcgggccgtg
     1681 tcggctgggg cgcgtcggga aggaacggcg gcttcgcctg catcggctcc cacaagctgt
     1741 cctacacgga gatgatcagg acctatgggc tgacggcgac gcagcacttc cacgccgcca
     1801 tggcggagtc ggtggacctc gtgcgcgaca attgccgccg cttcggcatc gacgcctgga
     1861 tccatggcgc gggcgaggtg acgctggcgc atctgcccaa ccgcgtggcg gagctgcgcg
     1921 gcgaccaggc cttcctcaag acgaccttcg gcgaggagac cgaacttctc tcggtggccg
     1981 atctcaaggc acaaggcctg tggggtccga ccttccatgg cgggctgagg ggcaagatcg
     2041 gcttcggcat ccatccgctg aactatgtgc gcggcctcgc ccgcgccgca gccgcggcgg
     2101 gtgccaaaat ccacgcatca tcgcgcctca cccgctggga ggagacggcg gcgggccat
//
LOCUS       DBNGVR010000485         3085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4305563, whole genome shotgun sequence.
ACCESSION   DBNGVR010000485 DBNGVR010000000
VERSION     DBNGVR010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4305563"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="AC16A0_10200"
     CDS             <1..642
                     /locus_tag="AC16A0_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105750.1"
                     /translation="MAANGSMLGALWNSLAVATIVAVVATAVGLPTAIALTRYRLRGG
                     GAIAAALMLPLVVPSIIFAVALLVILVRILNVELSLLTVGASHVFICLPFAIMVMMSR
                     LEGFDKSLEEASRDLGEDGWGTFRRVTLPLAWPAVISSLLMCFIISFDEFVMAFFLSG
                     TDPTLPVFLFGQLRFPNKLPGVLALGSLILLVSTILVVAAELLRRRGVQERPA"
     gene            639..1745
                     /locus_tag="AC16A0_10205"
     CDS             639..1745
                     /locus_tag="AC16A0_10205"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105751.1"
                     /translation="MMTAPMADTIISLKDVRRDFGPVAAVDGVSFDIARGEFFSMLGP
                     SGCGKTTLLRMLAGLDQPTAGSILLDGQPMAGVPANLRPTNMVFQSYAIFPHLNVVEN
                     VSYGLKRLKLARAEERARVDQALALVKLEGYEQRGAHQLSGGQRQRVALARALVCRPK
                     VLLLDEPLGALDKKLREEMQVELRSLQRSVGITFVFVTHDQEEALTLSDRIAVMARGK
                     VLQIDTATGLYERPTCREVAEFIGTMNLFAGRVTTIGPEISVDAGSIGTLRAPAGEAR
                     IAVGDAVHVAVRPEKLSLLTGKARTGANVVTGSLVASTYLGDRSRYLVQVPGVAAAVS
                     VSLQHDGQHAAGAALPGSAINLTWPASAGIVLTA"
     gene            1751..2335
                     /locus_tag="AC16A0_10210"
     CDS             1751..2335
                     /locus_tag="AC16A0_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3105752.1"
                     /translation="MGSEEDDADGAALIWPMERLSRLLRAQEHDRGLNPAQWEALRYL
                     ARANRFSNSPGALTRYLGATKGTISQTVMALERKGLVTKALRDGEKRSVTLALTPAGE
                     EMLAHDPWARLMKMAQELGGKTRRRMARGLRELLAAQLKAGGQSNFGSCGTCRYFREK
                     GRSHEAQGPHLCMYFEQALSRDETGRICIAHEER"
     gene            complement(2338..>3085)
                     /locus_tag="AC16A0_10215"
     CDS             complement(2338..>3085)
                     /locus_tag="AC16A0_10215"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR3105753.1"
                     /translation="ATTRRWGTGLEKVSIDRSSRSLDLDDASITENTRAAYPLAAIPN
                     ASRSGQAAIPKTIVMLTADAFGILPPIARLTPQQAMYHFLSGYTAKVAGIERGVTAPQ
                     ATFSTCFGAPFIPRHPSVYGKLLRDLIARHDVQCFLVNTGWTGGGHGQGQRMPITVTR
                     RLLNAALDGELATAAMRIDPNFGFAVPLAVSGVDPAILDPRGTWADPTAYDAAARKLV
                     GMFVKNFARFEDHVEADIRAASPMTAMAAE"
BASE COUNT          495 a          980 c         1049 g          561 t
ORIGIN      
        1 atggccgcca atggttcgat gctcggtgcc ctgtggaact cgctcgccgt cgcgaccatc
       61 gtcgcggtgg ttgcgaccgc cgtaggcctt ccgacggcca tcgcgctgac ccgctaccgg
      121 ctgcgcggcg gaggcgcgat tgcggctgcc ctcatgctgc cattggtggt gccttccatc
      181 atctttgccg tggcgctgct ggtcatcctc gtgcggatcc tcaatgtcga actgtctctg
      241 ctgacggtgg gcgcgtcgca tgtcttcatc tgcctgccct tcgccatcat ggtgatgatg
      301 tccaggctcg aaggcttcga caagagtctc gaggaagcct cgcgcgacct cggcgaggat
      361 ggctggggca cctttcgccg cgtgacgctg ccgcttgcct ggccggcggt catctccagc
      421 ctgctcatgt gcttcatcat ctcattcgac gagttcgtca tggctttctt cctcagcggc
      481 accgatccaa ccctgccggt ctttctcttc ggtcaattgc ggtttcccaa caagctgccg
      541 ggtgtcctgg cactgggctc gctgatcctg ctggtctcca cgattctcgt cgtggcggcg
      601 gaactgctac ggcgtcgcgg cgtgcaggag aggccggcat gatgacggca ccgatggcag
      661 atacgatcat ttcgctgaag gatgtccgcc gcgacttcgg accggtggcg gccgtcgatg
      721 gcgtcagttt cgacatcgcc cgcggcgagt tcttctccat gctggggcca tcgggctgcg
      781 gaaagaccac cctgctgcgc atgctggccg ggctcgatca gcccaccgcc ggttcgatct
      841 tgctggatgg ccagcccatg gccggcgtgc ctgccaatct ccggcccacc aacatggttt
      901 tccagagcta tgcgatcttc ccgcacctga atgtcgtcga gaacgtcagt tatggattga
      961 agcgcctgaa gcttgcacgg gcggaagaaa gggcgcgcgt cgaccaggcg ctggcgctgg
     1021 tgaaactcga aggctatgag cagcgcggcg cgcatcagct ctcgggtgga cagcgccagc
     1081 gcgtggcact ggcccgcgca ctggtgtgcc ggcccaaggt attgctcctc gacgagccgc
     1141 tgggcgcgct cgacaagaag ctgcgcgagg agatgcaggt ggagctccgc agcctgcagc
     1201 gcagcgtcgg catcaccttc gtcttcgtga cgcatgatca ggaggaggca ctcaccctgt
     1261 cggaccgcat tgccgtcatg gcgcgtggca aggtgctgca gatcgatacg gcgaccggcc
     1321 tctacgagcg gccgacctgc cgtgaggttg ccgaattcat cggcaccatg aatctctttg
     1381 ccggccgggt gaccacgatc ggccccgaga tctcggtcga tgcaggctcg atcggcaccc
     1441 tgcgcgcgcc ggcgggcgag gcaaggattg cggtcggaga cgccgtgcac gtggcggtga
     1501 gacccgagaa actgtcgctg ctcaccggaa aggccaggac cggcgccaat gtcgtaaccg
     1561 gcagtctcgt cgcatcgaca tatctcggcg accgcagccg ctacctcgtg caggttcccg
     1621 gcgtcgcggc agcggtgtcg gtgtcgctgc agcatgatgg ccagcatgcg gctggagcgg
     1681 cgctgcccgg cagcgcgatc aatctcacct ggccggcttc ggccggcatc gttctgaccg
     1741 cgtgacaggc atgggcagtg aagaagatga tgccgacggc gccgccctga tctggccgat
     1801 ggagcggctg tcacggctgt tgcgggcgca ggaacatgat cgcggtctca atccggcgca
     1861 atgggaagcg ctgcgctatc tggcgcgcgc caatcggttt tccaactcgc caggtgcgct
     1921 gacccgctat ctcggcgcca ccaagggcac catatcgcag accgtgatgg cactggagcg
     1981 caaggggttg gtgaccaagg ccctgcgcga cggcgagaag cgctcggtca cgctggccct
     2041 gactccggca ggagaggaga tgctcgccca tgatccctgg gcgcggctta tgaagatggc
     2101 gcaggagctg ggaggcaaga cacggcgccg catggcccgc ggccttcgcg aactcttggc
     2161 ggcccagctg aaggccggcg ggcagagcaa cttcggatcg tgcggcacct gccgctactt
     2221 ccgcgagaaa ggccgcagcc acgaggcgca ggggccgcat ctgtgcatgt acttcgagca
     2281 ggccctgagc cgcgacgaga ccgggcggat ctgcatcgcg cacgaagagc gctgaatcta
     2341 ttccgcggcc atggccgtca tcgggcttgc ggcgcggatg tccgcttcca catgatcctc
     2401 gaagcgcgcg aagttcttca cgaacatgcc caccagcttg cgggcggcgg catcataggc
     2461 cgtcggatcc gcccaggtgc cgcgcggatc gagaatggcg gggtccacgc cactgacggc
     2521 cagcggaacg gcaaagccaa aattgggatc gatgcgcatc gccgcggtgg ccagttcgcc
     2581 gtcgagtgcg gcattgagca gccggcgcgt gaccgtgatc ggcatgcgct ggccttggcc
     2641 atggccgccg ccggtccagc cggtgttcac caggaagcac tgaacatcgt gccgggcgat
     2701 caggtcgcgc agcagcttgc cgtagacgct gggatggcga gggatgaagg gtgcgccaaa
     2761 gcaggtggag aacgtcgcct gcggcgcggt cacgccgcgc tcgatgccgg ccaccttggc
     2821 cgtgtatccg gagaggaaat gatacatggc ctgttgcggc gtgagcctgg cgatgggcgg
     2881 cagaatgccg aaggcatctg ccgtcagcat cacgattgtc ttcgggattg ccgcctgacc
     2941 gctgcggctt gcattcggaa ttgctgcgag cggataggct gcgcgcgtgt tctcggtgat
     3001 gctggcgtcg tcgagatcga gtgaccgact gctgcggtcg atgctgacct tctcgagccc
     3061 ggtgccccag cgccgcgtgg tcgcg
//
LOCUS       DBNGVR010000486         2583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2736759, whole genome shotgun sequence.
ACCESSION   DBNGVR010000486 DBNGVR010000000
VERSION     DBNGVR010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2736759"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="AC16A0_10220"
     CDS             complement(<1..1130)
                     /locus_tag="AC16A0_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3105754.1"
                     /translation="MSENKGWRSETVAVHAGEAIDPATRASSPNLVMSVTFAPETLTG
                     FSARDEKNYGGFVYGRVSSPTVKQLEDKLAALEGGEMALAFASGVAAAHALICGRLSA
                     GDHLVLPEANYVGIAELARDTLPRFGIAVSYVDLNDLQAVADAIRPNTRMLWLETPAN
                     PTMKVCDIAALAELAHQKGVRDVAVDSTYATPIATQPLRLGADFVIHSLTKYIGGHGD
                     AMGGAVIGRKKDLDALNLEASVHFGGVMSPFNAWLILRGAGTLPIRMRAHQEAALAVA
                     KFLEAHPAVTRVFYPGLASHPQHEIAKRQMQNFSGMMTFQTRAPGPEIAARMIRELEV
                     IHYAVSLGHLRSLICWIGTDDIQQSTFRHDAAALARYREFAG"
     gene            1351..>2583
                     /locus_tag="AC16A0_10225"
     CDS             1351..>2583
                     /locus_tag="AC16A0_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105755.1"
                     /translation="MAGYLVLAIIAIGFALATLSVDPDSLPQVTLDDRTAAAQAALQK
                     NFPGIDSAILAVVSGENRDAGRDGALELATALGGRPDLFASAFVPGTGAFYSRYGLLY
                     LPAAEIAARVSDLIQKQPLDLAVSAAPDLQGLAALISEIGRAVEQGRSPPGLAGLLMA
                     TSDVIEAEVAGEPRALNWTELAGLTPGNGSSTWFVVATPVAGMDNEAAAFARSLTASA
                     TGVTWLFPRPTQGEPRQMIRNFAVPVGLAIVVTLTVLLAGIGRVRLAIPLVLNAIVTV
                     ALAAAIAAILNARLDGVTWSFAAAVVAPALLLSSILVLAHIQARLRGAGVRQSIMLAS
                     QRRGGMQCAVAVIFAVFWLSWLVRQIPSLTEFAVVALFGTGVALAAALTLVPAALAAF
                     DQDDDELPLHWFDVAVAMP"
BASE COUNT          417 a          835 c          854 g          477 t
ORIGIN      
        1 tcgccggcaa actcgcgata ccgcgcaagc gccgcggcat cgtggcggaa ggtcgactgt
       61 tggatgtcgt cggttccgat ccagcagatc aggctgcgaa gatggccgag cgagacggca
      121 taatggatca cctcgagctc cctgatcatg cgtgccgcga tttcaggacc cggcgccctg
      181 gtctggaatg tcatcattcc cgagaagttc tgcatctgcc ttttcgcaat ctcgtgctgc
      241 ggatgcgagg cgaggccggg atagaagacg cgggtgacgg cgggatgcgc ctccaggaac
      301 ttcgccactg cgagtgccgc ctcctgatgg gcgcgcatcc ggatcggcag cgtgcccgcg
      361 ccacgcagga taagccaggc attgaaaggc gacatcacgc cgccgaagtg aaccgaggcc
      421 tcgaggttga gcgcatcgag atccttcttg cggccgatga ccgcgccgcc catcgcatcg
      481 ccatgcccgc cgatgtattt cgtgagtgaa tggatcacga agtcggcgcc gaggcgcagc
      541 ggctgggtgg cgattggcgt ggcataggtg gagtccacgg caacatcgcg cacccctttc
      601 tggtgcgcca gctcggcaag ggccgcgatg tcgcagacct tcatggtcgg attggccgga
      661 gtctccagcc acagcattct cgtgttgggc cggatggcgt cggccacggc ctgcaggtca
      721 ttgaggtcga cataggagac agcgatgccg aagcggggca gggtgtcgcg ggccagttcg
      781 gcaatgccca cgtagttcgc ctcgggcagc accagatgat cgccggcgct gagccggccg
      841 cagatcagcg catgggctgc cgccacgccc gaggcgaagg caagagccat ctcgccaccc
      901 tccagggcgg caagcttgtc ctccagctgc ttcaccgtcg ggctcgagac gcgcccatag
      961 acgaagccgc catagttctt ttcatcgcgg gcagaaaagc ccgtgagggt ctcgggcgca
     1021 aaggtgacgg acatcacgag attgggagag gaagcccggg ttgccgggtc gatcgcctcg
     1081 ccggcatgga cggccacggt ttcactgcgc cagcccttgt tttcgctcat cagttcatca
     1141 ctttctttac ttgttcaagg gcttatgatg cagcgcggtg tgcgaatccg caaccggtgc
     1201 gaatgcatgg cggagagtct taaacgtatg gtgcaatggg accaacccca ctggagcggc
     1261 cggccgaagg agccgcaacg gcgcccgccg atccgcccca acagcttcgc cctgatcgca
     1321 cgtgcgtgcg cgcgctacag catggccgtg atggccgggt atcttgttct cgccatcatc
     1381 gccatcggct ttgcgctggc cacgctctcg gtggacccgg acagccttcc gcaagtcacc
     1441 ctcgatgatc gcacggcggc tgcacaggcc gcgctccaga agaattttcc gggcatcgat
     1501 tccgccattc tggcggtcgt gtcaggcgaa aaccgtgacg caggacgcga cggtgcactc
     1561 gaactggcga ccgcgctcgg cggccggccg gacctctttg catctgcctt cgtgccaggc
     1621 accggggcct tctacagccg ctatggcctg ctctatcttc ccgccgcgga gatcgccgcc
     1681 cgcgtttccg atctcatcca gaagcaaccg ctcgaccttg ccgtcagcgc tgcgcctgat
     1741 ctgcagggac tggcggccct gatctcggaa atcgggcgtg ccgtggagca ggggcgttcg
     1801 ccgcccggcc tggccggact gctcatggca acgtcggacg tgatcgaggc ggaggtggcg
     1861 ggcgaacccc gcgcactgaa ctggacggaa cttgcggggc tgacgcctgg aaacggcagc
     1921 agcacctggt tcgtcgtcgc gacacccgtc gccgggatgg acaatgaagc ggcggccttc
     1981 gcccgatcgc tgacggcaag cgccaccggg gtgacctggc tgtttccgcg gccaacccag
     2041 ggcgagcccc gccagatgat ccgcaatttc gccgttcccg ttggcctggc cattgtggtg
     2101 acgctgacag tgctgctggc cggcatcggg cgggtgcggc tcgccattcc actcgttctg
     2161 aacgccatcg tcaccgttgc cctggcggct gccatcgccg ccatcctcaa cgcccggctg
     2221 gacggcgtca catggtcctt cgcggctgcc gtggtggcgc cagccctgct gttgtcatca
     2281 atactggtgc tggcccatat acaggcccga ttgcgcgggg ccggcgtgcg ccagtcgatc
     2341 atgctggcca gccaaaggcg tggcggcatg caatgtgcgg tcgcggtgat ctttgcggtg
     2401 ttctggctgt cgtggctggt ccggcagatt ccgtcgctga ccgagtttgc agtcgtcgca
     2461 ttgtttggaa cgggcgttgc gcttgcggcc gcgctgaccc tggttccagc ggcgcttgcc
     2521 gcctttgacc aggacgacga cgagttgccg cttcactggt tcgatgtcgc ggtggccatg
     2581 ccc
//
LOCUS       DBNGVR010000487         2916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5487265, whole genome shotgun sequence.
ACCESSION   DBNGVR010000487 DBNGVR010000000
VERSION     DBNGVR010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5487265"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="AC16A0_10230"
     CDS             <1..554
                     /locus_tag="AC16A0_10230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260204.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HIR3105756.1"
                     /translation="ATIMAILNANYIARRLAPHFPIVYTGPGGFIAHECIIDLRWLKE
                     RTGLSVEDVAKRLVDYGFHAPTMSFPVADTLMIEPTESESKRELDRFCDAMIEIRKEI
                     AEVEEGRADRSDNVLRNAPHTHRLLLGDWKHPYSKEKAFFPLKGYPNDKYWPPVARVD
                     NVYGDRNLVCTCPPMANYMDAAE"
     gene            608..1489
                     /locus_tag="AC16A0_10235"
     CDS             608..1489
                     /locus_tag="AC16A0_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HIR3105757.1"
                     /translation="MRFQTAAIIAFLASAPAAGAHDLKHLPLGDTLRSKAPAVGHIWP
                     CRIDPRAGGAQVNGPWIDLEAGTFDKTAKPVVGGKVTWPNVFEIRRDGDRRVFFTNAL
                     PDHGTGVFPIRPGSEAYKYDRNPHHIQPQRMGFSLPADPELAESPTCAPGVVGILLTG
                     VPLYSALDAPGRDAVAHEVQDSCEGHPQPAGIYHYHSLSPCALSRQQPGQHGQLAGYA
                     VDGFGIFGPCGESGEALTSADLDACHGHSHEIDWDGERRVMYHYHGTPDFPYTVGCLR
                     GAHSREVVRILSGPPPR"
     gene            complement(1609..>2916)
                     /locus_tag="AC16A0_10240"
     CDS             complement(1609..>2916)
                     /locus_tag="AC16A0_10240"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3105758.1"
                     /translation="KREGLVLAFWARTIALVFISAFLFFVVQWPVVLFPQLFLAAFFV
                     VGWLQQRYGRVGRSRIELLLAFADIALLTAVVVIPNPLNPDDLPLPLQYHFDSFIYAF
                     VMLAGVTLSLHWRTINLLGFGVPLIWLAAAAAVWFFFQPLPHLTALADQMFGTRTDLN
                     DLFDPNEINWDQRVQEALVYCLVALILGLNGMRGERLLLQFAEAERKRTNLSRYFSPN
                     VTAELARADDALSQSRTIPVAVMFVDIVGFTRFASAVSAAEAMETLRSFFARMERAVF
                     AHNGTLDKYLGDGLMATFGTPVPGPDSAVNALRCGAAMQSAVEDWNRERAQRGLPPFR
                     IGVGVHFGEVAFGNIGNSRLELAVIGDTVNVASRLESLTRELDATIAVSREVLEQAIA
                     VAGPQDPCLAGFHPVGPRKVQGTSKWIEVAAISRPPLLPPAAA"
BASE COUNT          535 a          943 c          985 g          453 t
ORIGIN      
        1 aggcgacgat catggcgatc ctcaatgcca attacatcgc aaggcgcctc gcgccgcatt
       61 ttcccatcgt ctacaccggc cccggcggct tcatcgcgca tgagtgcatc atcgacctgc
      121 gctggctgaa ggaacgcacc ggcctttcgg tcgaggacgt ggccaagcgc ctggtggact
      181 atggcttcca tgcgccgacc atgtccttcc cggtggccga cacgctgatg atcgaaccca
      241 ccgagtccga gagcaagcga gagttggacc gcttctgcga cgccatgatc gagatccgca
      301 aggagatcgc cgaggtggag gagggccggg ccgaccgcag cgacaatgtg ctccggaatg
      361 cgccgcacac gcaccgcctg ctgctgggcg actggaagca cccctattcg aaggaaaagg
      421 ccttctttcc gctgaaggga tatccgaacg acaagtactg gccgccggtg gcccgcgtcg
      481 ataacgttta cggcgaccgc aacctcgtct gcacctgtcc gcccatggcg aattacatgg
      541 acgcggcgga gtaatccgcg acggaatttg acgtatcgga acgtctgaga tggcaaggga
      601 ggacctcatg cgttttcaga cggccgccat cattgcgttt ctcgcctccg caccagcggc
      661 aggcgcgcac gacctcaagc atctgccact tggtgacacg ctccgatcga aggcgccggc
      721 ggtgggccat atctggccct gccgcatcga tccccgggcc ggcggcgccc aggtcaatgg
      781 tccctggatc gatctggagg ccggcacctt cgacaagacc gccaaaccgg tggtgggtgg
      841 caaggtgaca tggcccaatg tcttcgagat ccggcgcgac ggcgaccgcc gggtcttctt
      901 caccaatgcg ctgccggacc acggcaccgg ggtctttccg atccggccgg gcagcgaggc
      961 ctacaagtat gaccgcaatc cgcatcacat ccagccgcag cgcatgggtt tcagcctgcc
     1021 ggccgatccg gagctggcgg agagcccgac ctgcgcgccc ggcgtggtgg gcatcctgct
     1081 gaccggcgtg ccgctgtaca gcgcgctgga tgcgccgggc cgcgatgccg tggcccatga
     1141 ggtgcaggac agctgcgaag gccatcccca gccggcgggc atctatcact atcactcgct
     1201 cagtccctgc gcgctcagcc ggcagcagcc gggacagcac gggcaactgg ccggctatgc
     1261 cgtcgatggc ttcggcatct tcggcccctg cggagagtcg ggcgaagcgc tgaccagtgc
     1321 cgatctcgac gcctgtcacg ggcacagcca tgagatcgac tgggatggcg aaagacgcgt
     1381 catgtatcac tatcacggta cgcccgattt tccctacacg gtgggatgcc tgcggggcgc
     1441 gcacagccgc gaggtggtcc gcatcctcag cggtccgccg ccgcggtagg gctgagatgc
     1501 gggtcgacgc cttgcgtcat cccggtcgtt ggagcgcagc gatggctgca ggctgtatcc
     1561 ttgcgccagg ccccggttct cgccggggtg acggcggagg agcggaccct acgccgctgc
     1621 cgggggcagc aaaggcgggc ggctgatcgc ggcgacctcg atccacttgc tggtgccctg
     1681 caccttgcgg gggcccacgg gatggaagcc ggccaggcaa ggatcctgtg gcccggccac
     1741 ggcgatggcc tgctccagca cctcgcgcga gacggcgatg gtggcatcca gttcgcgcgt
     1801 cagcgactcc agccggctgg cgacattcac cgtgtcgccg atcaccgcga gctcgaggcg
     1861 gctgttgccg atgttgccga aggccacctc gccaaaatgc acgccgacgc cgatacggaa
     1921 gggcggcagg ccgcgctgtg ccctttcccg gttccagtct tcgaccgcgc tctgcatggc
     1981 ggccccgcag cgcaaggcat tgacggcgct gtcggggccg ggcaccggcg tgccgaaggt
     2041 cgccatgagc ccgtcgccca gatacttgtc cagcgttccg ttgtgcgcga agacggcgcg
     2101 ctccatgcgg gcaaagaagc tgcgcagcgt ctccatggcc tcggctgccg acacggcgga
     2161 ggcgaagcgg gtgaagccca cgatgtcgac gaacatcacc gccaccggaa tggtgcggct
     2221 ctggctcagc gcgtcatcgg cgcgcgccag ctcggccgtc acgttgggcg agaagtagcg
     2281 cgacaggttg gtgcgcttgc gctcggcttc ggcgaactgc aagagcagcc tctcgccgcg
     2341 catgccgttg aggccgagga tcagcgccac caggcaatag acaagggcct cctgcacgcg
     2401 ctggtcccag ttgatctcgt tgggatcgaa caggtcattg aggtcggtgc gtgttccgaa
     2461 catctgatct gcgagcgccg tcagatgcgg gagcggctga aagaagaacc agaccgcagc
     2521 ggccgcggca agccagatca gcgggacgcc gaaaccgagc aggttgatgg tgcgccagtg
     2581 caatgacagc gtcacgccgg ccagcatgac gaaggcatag atgaagctgt cgaagtggta
     2641 ctgcaatggc aggggaagat cgtcgggatt gagcgggttg ggaatgacga cgacagcggt
     2701 cagcagcgcg atgtcggcga aggcgagcag cagctcgatc cgcgagcgcc cgacccggcc
     2761 atagcgctgc tgcagccatc ccaccacgaa gaaggctgcg agaaacagtt gtggaaacag
     2821 cacgacgggc cattgcacga cgaagaacag gaaggccgag atgaagacca gggcgatggt
     2881 tcgggcccag aaggcgagga ccaggccctc gcgctt
//
LOCUS       DBNGVR010000488         1723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2541126, whole genome shotgun sequence.
ACCESSION   DBNGVR010000488 DBNGVR010000000
VERSION     DBNGVR010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2541126"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC16A0_10245"
     CDS             <1..233
                     /locus_tag="AC16A0_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3105759.1"
                     /translation="GDMALGEAVGRLAAKHSAMLLANHGPVVAGSSLSAAVDAIEELE
                     ETARLYLLLHGRPTRYLSPEQIADLKRRFPPA"
     gene            246..1193
                     /gene="speB"
                     /locus_tag="AC16A0_10250"
     CDS             246..1193
                     /gene="speB"
                     /locus_tag="AC16A0_10250"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3105760.1"
                     /translation="MTEFNQPLGGNEMARFSGPATMMRLPAQTTAEGLDACFVGVPMD
                     IGTSNRSGTRHGPRQIRAESCMLRPYNMATRAKPFESLQVADIGDVAINTFDLKKTVG
                     IIEKAYDKILAQFCIPLTLGGDHTLTLPILRAMKKVHGPVAMIHVDAHADINDEMFGE
                     KIAHGTPFRRAVEEGLLACDKVFQIGLRGSGYSPEDFDWPRKQGFTVVEAEKCWYTSL
                     APLMEEVRAKIGDHPVYLTYDIDSLDPAFAPGTGTVEVGGLTTWQGLEIIRGCRGLNL
                     VGGDLVEVSPPFDPSGNTALIGANFLFEMLCVLPGVKYG"
     gene            1317..>1723
                     /locus_tag="AC16A0_10255"
     CDS             1317..>1723
                     /locus_tag="AC16A0_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105761.1"
                     /translation="MDDARHSLQDEMARAIAASHLSPHVREILSLRFARSLEDLFRPL
                     ADLYGSHPEYGRFCRQLIAALVAAQEERPAGLQTLDLRRDLEPDWFLREKMVGYVFYI
                     DRFNGSLGGVLDRLDYLEELGVTYVHFMPCLRPR"
BASE COUNT          309 a          565 c          528 g          321 t
ORIGIN      
        1 cgggcgacat ggccttgggc gaggcggtgg ggaggctggc ggcgaagcat tcggccatgc
       61 tcctggcaaa tcacggtcct gtcgtcgccg gatcctcgct ttcggctgca gtcgatgcga
      121 tcgaagagct ggaagagaca gcacggctct atctgcttct ccacggccgc ccgacacgct
      181 atctgtcgcc ggagcagatc gccgacctca agcggcgctt tccgcccgcc tgacaggaga
      241 agaccatgac agagttcaac cagccgctgg gcggcaacga gatggcgcga ttctccggtc
      301 ccgccaccat gatgcggctt ccggcccaga cgacggcgga ggggctggac gcctgcttcg
      361 tcggcgtgcc catggacatt ggcacctcca accgctcggg cacgcggcat gggccgcgac
      421 agatccgcgc cgagtcctgc atgctcaggc cctacaacat ggcgacgcgc gccaagccct
      481 tcgagtcatt gcaggtcgcg gacatcggcg atgtcgccat caacaccttc gacctcaaga
      541 agacggtcgg catcatcgag aaggcctatg acaagatcct ggcccagttc tgcattccgc
      601 tgacgctggg gggcgaccac acgctgacgc tgcccatcct gcgcgccatg aagaaggtgc
      661 acgggccggt cgccatgatc catgtcgacg cccatgccga catcaacgac gagatgtttg
      721 gcgagaagat cgcgcatggt acgcccttcc gccgcgccgt cgaggaaggg ctgctcgcct
      781 gcgacaaggt cttccagatc ggcttgcgcg ggtccggcta ttcgcccgag gacttcgact
      841 ggccgcggaa gcagggcttc accgtcgtgg aagctgagaa gtgctggtac acgtcgctcg
      901 cgccgctgat ggaagaggtg cgggcgaaga tcggggatca tccggtctac ctcacttatg
      961 atatcgacag cctcgatccc gcctttgcgc ccggcaccgg aaccgtggaa gtcggcggcc
     1021 tcaccacctg gcagggcctc gagatcattc gcggctgccg cggactgaat ctggtgggcg
     1081 gcgatctcgt cgaggtctcg ccgcccttcg atccttccgg caataccgcg ctgatcggcg
     1141 ccaatttcct cttcgagatg ctctgcgtgc tgcccggcgt caaatacggc tgaattgaaa
     1201 acgctttgac tgcgaagccc tctgttttca tcacaacata aggaattcac gttgactctc
     1261 ccacacaagg cgggtcataa cggcagtaat tcaaagcgct ttggaagtcg tttccgatgg
     1321 atgatgccag gcacagtctg caggacgaga tggcgcgggc catcgcggca tcgcacctct
     1381 ccccgcatgt ccgcgagatc ctgtccctgc gcttcgcgcg cagcctcgag gatctcttcc
     1441 gcccgcttgc cgacctctat gggtcacatc cggaatatgg gcgcttctgc cggcagctga
     1501 tcgccgcgct ggtcgcagca caggaagaac ggcccgccgg ccttcagacg ctggacctgc
     1561 gccgcgatct cgaacccgac tggttcctgc gcgagaagat ggtgggctac gtcttctaca
     1621 tcgaccgctt caatggctcg cttggcggtg tgctcgacag gctggactat ctcgaagagc
     1681 tgggcgtcac ctatgtccac ttcatgccct gcctcaggcc gcg
//
LOCUS       DBNGVR010000489         2678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2541837, whole genome shotgun sequence.
ACCESSION   DBNGVR010000489 DBNGVR010000000
VERSION     DBNGVR010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2541837"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="AC16A0_10260"
     CDS             complement(<1..499)
                     /locus_tag="AC16A0_10260"
                     /inference="COORDINATES: protein motif:HMM:NF025244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HIR3105762.1"
                     /translation="MPDRVARVLAAIDAANAADPHLAPDGQPQALLYGRRMSDNLARF
                     APQASEALQIAARGQHIERWTIPRASYPEGRIAYLTWRKDLQKHHAARCGAIMTAEGY
                     GADEIARVGALLRKERLKQDPDVQTLEDVICLVFLAHEADAFIAKHDDAKVRDILAKT
                     ARKMSP"
     gene            complement(492..1274)
                     /gene="otnI"
                     /locus_tag="AC16A0_10265"
     CDS             complement(492..1274)
                     /gene="otnI"
                     /locus_tag="AC16A0_10265"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HIR3105763.1"
                     /translation="MPRFAANLSMLFNEVPFLDRFAAAAKGGFKGVEYVGPYDYSPEQ
                     IADLLQRNGLTQVLFNMPAGNWAAGERGIGCHPDRVGEFQDGVATTIRYAKVLGNRQV
                     NCLAGLLPKGVSREAAEETFVANLRFAAAELKKAGILLIAEPINFHDMPGFFLNTSAQ
                     AIAIFDRVGSDNLKLQYDIYHMQRMEGELAKTIERLFPRIAHFQIAGNPNRTEPDRGE
                     INYPFLYDLIDRLGYTGWIGAEYRPVKTTAEGLGWLAPYRHA"
     gene            complement(1277..2572)
                     /locus_tag="AC16A0_10270"
     CDS             complement(1277..2572)
                     /locus_tag="AC16A0_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3105764.1"
                     /translation="MLDLLLRNATLPDGRSGQDIACKDGRIVEVQPKIDGAARATVDA
                     KGYLVSPPFIDSHFHMDATLSLGLPRLNMSGTLLEGISLWGELKPILTHEAVVERALR
                     YCDLAVTQGLLAIRTHVDVCDDRLLCVEALLEVRRKIAPYIDLQLVAFPQDGFYRDPH
                     ARRNLIRALDMGVDVVGGIPHFERTMTDGAASLKELCEIAANRGLLVDIHCDETDDPL
                     SRHVEQLAYETQRLGLQGRVVGSHLTSMHSMDNYYVSKLIPLMAEAELHATPNPLINI
                     TLQGRHDTYPKRRGMTRVPELRAAGINVSFGHDCVMDPWYPLGQGDMLEAAYMGIHAA
                     PMTSREAMRWAFDAVTVNPAKAMHLQDYGLEKGCNADFVVLQAKDPIDAIRLKANRLT
                     VVRRGKVIAETPERVSALHLAGRPATINGADFAPRAGGR"
BASE COUNT          460 a          825 c          917 g          476 t
ORIGIN      
        1 cgggcgacat cttgcgcgcg gtcttggcga ggatgtcgcg caccttggca tcgtcgtgct
       61 tcgcgatgaa ggcgtcggcc tcatgcgcga ggaagaccag gcagatcacg tcttcgaggg
      121 tctgcacgtc ggggtcctgc ttgagccgtt ccttgcgcag caacgcgccg actcgcgcga
      181 tctcgtccgc gccatagcct tccgccgtca tgatggcgcc gcagcgcgcg gcgtggtgct
      241 tctgcagatc cttgcgccag gtgagatagg caatacggcc ttcgggatag gaggcgcgcg
      301 gaatggtcca gcgctcaatg tgctggccgc gtgccgcaat ctgcaaggct tctgaagcct
      361 gcggtgcaaa tcgcgcaagg ttatcggaca tgcggcggcc gtagagcaag gcctgcggct
      421 ggccatccgg cgcgagatgg ggatcggcgg cattcgccgc atcgatggcg gccaggacgc
      481 gagcgacccg gtcaggcatg gcggtagggc gcgagccagc cgagaccttc ggcggtggtc
      541 ttcaccgggc ggtactcggc gccgatccac ccggtgtagc caagcctgtc gatgagatcg
      601 tagaggaagg gatagttgat ctcgccgcgg tcaggttcgg tgcgattggg attccccgcg
      661 atctggaaat gcgcgatgcg cgggaagagc cgctcgatgg tcttggccag ttcgccttcc
      721 atgcgctgca tgtgatagat gtcgtactgg agcttgagat tgtcagagcc cacccggtcg
      781 aagatcgcga tggcctgggc cgaggtgttg aggaagaaac ccggcatgtc gtgaaagttg
      841 atgggctcgg cgatgagcag gatgccggct ttcttcagtt cggctgcggc gaagcgcagg
      901 tttgcgacga aggtctcctc cgcagcctcg cgcgacacgc ctttcggcag cagaccagcg
      961 aggcagttca cctgcctgtt gcccagcacc ttcgcatagc ggatcgtggt cgccacgccg
     1021 tcctggaact cgcccacgcg gtccggatgg cagccgatgc cgcgctcgcc cgcagcccag
     1081 ttgccggcgg gcatgttgaa gaggacctgg gtcaggccat tgcgctgcag caggtccgcg
     1141 atctgttccg gcgaatagtc ataaggaccg acatactcga cgcccttgaa gccgcccttc
     1201 gctgccgcgg cgaaacggtc aagaaagggc acctcgttga acagcatgct gagattggcg
     1261 gcgaagcgcg gcatgatcag cgcccgcccg cacgcggcgc gaagtccgcg ccattgatgg
     1321 tggcgggacg gcccgcgaga tgaagcgcgc tcacacgctc cggcgtttcg gcaatcacct
     1381 tgccccggcg caccaccgtg agacggttgg ccttgaggcg gatggcatcg atgggatcct
     1441 tcgcctgcag caccacgaag tcggcgttgc agcccttctc caggccgtag tcctgcaaat
     1501 gcatggcctt ggcgggattg accgtcaccg catcgaaggc ccagcgcatg gcctcgcggc
     1561 tggtcatggg cgcggcatgg atgcccatgt aggcggcctc gagcatgtcg ccctgcccca
     1621 gcggatacca gggatccata acgcagtcgt ggccgaagga gacgttgatg ccagcggcgc
     1681 gcagttccgg cacgcgcgtc atgccgcggc gcttcggata ggtgtcgtgg cggccctgca
     1741 gcgtgatgtt gatcagcgga ttgggcgtgg catggagttc ggcctccgcc atcagcggga
     1801 tcagcttcga gacatagtag ttgtccatgg aatgcatgga ggtgagatgc gatcccacca
     1861 cgcggccctg cagtccgagg cgctgcgtct cgtaggcgag ctgctcgaca tggcgcgaca
     1921 gcggatcgtc cgtctcgtcg cagtggatgt cgaccagaag cccacggtta gcggcgatct
     1981 cgcagagttc cttcaggctg gctgcgccat cggtcatggt gcgctcgaaa tgcgggattc
     2041 cgcccaccac gtcgacgccc atgtcgagcg cgcggatgag attccgcctt gcatgcggat
     2101 cgcggtagaa cccgtcctgc gggaaggcca cgagctgcag atcgatgtag ggcgcgatct
     2161 tcctgcgcac ctccagcaat gcctcgacgc agagcagacg gtcgtcgcag acatcgacat
     2221 gggtacggat ggccagcagg ccctgcgtca cggcaaggtc gcagtagcgc agcgcgcgct
     2281 cgacaaccgc ttcatgagtc aggatcggct tgagttcgcc ccacagcgag atgccctcaa
     2341 gcagcgtgcc cgacatgttg agccgcggaa ggccaagcga cagcgtggca tccatgtgga
     2401 agtgggagtc gatgaagggc ggcgagacga gatagccctt cgcatcgacg gtcgcgcgcg
     2461 cggcgccgtc gatcttcggc tgcacctcga caatgcgccc gtccttgcag gcgatgtcct
     2521 gcccgctcct gccgtcggga agcgtggcgt tgcgcagcag cagatcgagc atgattgtcc
     2581 ccgtcgtcag cgggtgggaa tgggcttctc gccgcggtag tcgtagaagc cgcggttggt
     2641 cttgcggcca agccagccgg cctcgacata cttcacca
//
LOCUS       DBNGVR010000490         2295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2344149, whole genome shotgun sequence.
ACCESSION   DBNGVR010000490 DBNGVR010000000
VERSION     DBNGVR010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2344149"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="ccoP"
                     /locus_tag="AC16A0_10275"
     CDS             <1..665
                     /gene="ccoP"
                     /locus_tag="AC16A0_10275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR3105765.1"
                     /translation="FDKYAKMDVAAVAADPQAKEMGQRLFLTYCAQCHGSDAGGATSF
                     PSLKDGDWLWGGAPEKIKETITAGRTGVMTPKGTKPDMDGEQVKDVVHYVRSLSGLSA
                     DSIRVQRGKDLFPQACAACHGPEAKGNPDAGYPNLTDKIWLHGSSEAKMTEIVTKGMQ
                     NQMPAFGEFLGPAKVHLLTAYVWGLGGGAKGEAKPAAAPAAEQAGMSGMGGMSGMAAD
                     KK"
     gene            739..1770
                     /gene="ccoG"
                     /locus_tag="AC16A0_10280"
     CDS             739..1770
                     /gene="ccoG"
                     /locus_tag="AC16A0_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HIR3105766.1"
                     /translation="MTTETSSSSATTEQAQEIALFEVHKKIQVRSVSGIFATWRWALV
                     WFTQLLYYGLPWLQWNGRQAVLLDLVERKFYIFGLVFWPQDVIFLAGLLIISAYSLFL
                     FTAVGGRLWCGYACPQTVYTEIFLWIEQKIEGDRNARMKLDAAPMSARKFMIRGSRYA
                     AWVAVALWTGFTFVGYFTPIKTLWGELISFNLGPWQTFWLFFYSAFTYAFAGHLREQV
                     CKYMCPYARFQGVMFDPDTLTITYDVERGEPRGTRKKGADYKNQGKGDCVDCGICVQV
                     CPTGIDIRKGLQYECIGCAACIDACDQVMEKMSYPKGLIRYSTEHAIAQHWGWKDIAG
                     HIVRPRVVI"
     gene            1783..2160
                     /locus_tag="AC16A0_10285"
     CDS             1783..2160
                     /locus_tag="AC16A0_10285"
                     /inference="COORDINATES: protein motif:HMM:NF023045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HIR3105767.1"
                     /translation="MAITAAWFWGLANKPVLRVDVIRDRATLAREVEDGLIENLYRLQ
                     VMNVSEKAERYSMSVTGLPGIRLAGETEIEVPAASNKSILVQVRVPPESGKQGSNVVF
                     FDVKSTRNDKIAVHEKATFLIPR"
BASE COUNT          407 a          747 c          710 g          431 t
ORIGIN      
        1 tgttcgacaa gtacgccaag atggacgtcg ccgcggtcgc cgccgatccg caggccaagg
       61 aaatgggcca gcgcctgttc ctgacctact gcgcgcaatg ccacggctcc gacgccggcg
      121 gcgccaccag tttccccagc ctcaaggatg gcgactggct ctggggcggc gctcccgaga
      181 agatcaagga aaccatcacc gccggtcgta ccggcgtgat gacgccgaag ggcaccaagc
      241 ccgacatgga tggcgagcag gtcaaggacg tcgtgcatta tgtccgttcg ctctccggcc
      301 tctccgccga ttccatccgc gtccagcgcg gcaaggatct ctttccccag gcttgcgccg
      361 cctgccacgg tccggaggcc aagggcaatc ccgacgccgg ctacccgaac ctgaccgaca
      421 agatctggct gcatggctcg tcggaagcca agatgaccga gatcgtcacc aagggcatgc
      481 agaaccagat gccggccttc ggcgagttcc tcggcccggc caaggtccac ctgctgaccg
      541 cctatgtctg gggcctcggc ggcggcgcca agggtgaagc caagcccgct gcggctccgg
      601 ctgcggagca ggccggtatg agcggcatgg ggggtatgtc gggcatggcc gccgacaaga
      661 agtaaaattg cagcaagcgc tccccggcag gagggccggg gagcggcttc acccctgatt
      721 gttcattcga gcgtcatcat gaccactgaa acctccagtt cctccgcgac gaccgagcaa
      781 gcccaggaaa tcgccctttt cgaagtccac aagaagatcc aggtccgctc ggtgagcggg
      841 atcttcgcca cctggcgctg ggctctcgtc tggttcactc aactcctcta ttacggcctg
      901 ccctggttgc agtggaacgg ccgtcaggcc gtgctgctcg acctcgtcga gcgcaagttc
      961 tacatcttcg gcctggtgtt ctggccgcag gacgtcatct tcctggccgg cctgctgatc
     1021 atctcggcct attcgctgtt cctgttcacc gccgtcggcg gtcgcctgtg gtgcggctat
     1081 gcctgcccgc agaccgtcta tacggaaatc ttcctctgga tcgagcagaa gatcgagggc
     1141 gaccgcaacg cgcgcatgaa gctcgatgcg gcgccgatgt cggcccgcaa gttcatgatt
     1201 cgcggctcgc gctatgcggc ctgggtcgcg gtggcactgt ggaccggctt caccttcgtc
     1261 ggctacttca cgccgatcaa gaccctctgg ggcgaactga tctctttcaa cctcggcccc
     1321 tggcagacgt tctggctgtt cttctacagc gccttcacct atgccttcgc cggccatctg
     1381 cgcgagcagg tctgcaagta catgtgcccc tacgcccgct tccagggcgt gatgttcgac
     1441 cccgatacgc tgaccatcac ctacgacgtg gaacgcggcg agccgcgcgg cacgcgcaag
     1501 aagggggccg attacaaaaa ccagggcaag ggtgactgcg tcgattgcgg catctgcgtc
     1561 caggtctgtc cgaccggcat cgatatccgc aagggcctgc agtacgagtg catcggttgc
     1621 gccgcctgca tcgatgcctg cgatcaggtg atggaaaaga tgtcctatcc gaagggcctg
     1681 atccgctatt cgacggagca cgccatcgcc cagcactggg gctggaagga catcgccggc
     1741 cacatcgtgc gcccgcgcgt ggtgatctag accgtcattc tcgtggcgat caccgccgct
     1801 tggttctggg ggctggccaa caagcctgtg ctgcgggtgg atgtgatccg cgatcgtgcc
     1861 acgctggcgc gcgaggtcga ggacggactg atcgagaatc tgtatcgcct gcaggtcatg
     1921 aacgtttcgg aaaaggcgga acgctattcg atgagcgtca cgggcctccc ggggattcgc
     1981 ctcgcgggtg agacggagat cgaggttcct gccgcgtcca acaagagcat cctggtccag
     2041 gttcgcgtgc cgcccgaatc gggcaagcag ggctcgaacg tggtcttctt cgacgtcaaa
     2101 tcgacccgaa acgacaagat cgccgtgcat gagaaggcga ccttcctgat tccccggtga
     2161 tccgcacgat gagcatccct ctgaaatctt cgcagccgtg gtatcgcgag ccctggccct
     2221 ggctgctgat gctgggtccc ttcgtggtga tcctcgccgg catctatacc gcctggctgg
     2281 cgatatcgac cagtg
//
LOCUS       DBNGVR010000491         2132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3719936, whole genome shotgun sequence.
ACCESSION   DBNGVR010000491 DBNGVR010000000
VERSION     DBNGVR010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3719936"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="trpE"
                     /locus_tag="AC16A0_10290"
     CDS             <1..1248
                     /gene="trpE"
                     /locus_tag="AC16A0_10290"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR3105768.1"
                     /translation="GPFETIALPPLQALRALLAESRIKLPEDAPPMAAGVFGYMGYDM
                     VRLMEDLPPPNPDTLGLPDAVLIRPTIMAIFDSVRDEVTVTSPVYPSIAVSSRAAYAR
                     AQERISEIVDALDRPLDHIALRDEDIAAISPPRSNTAPEDYKAMVLKAKEYIAAGDIF
                     QVVLSQRFETEFSLPPFSLYRSLRRVNPSPFLYYLDFDRFAVIGSSPEILVRVRDGKV
                     SIRPIAGTRRRGATPAEDRALAAELLADPKELAEHLMLLDLGRNDVGRVAEIGTIKVT
                     DKFILEYYSQVMHIVSNVEGKLAQKHDAIDALVAGFPAGTVSGAPKVRAMAIIDELET
                     SKRGVYAGCVGYFSADGEMDTCIVLRTAIVKDGKMYVQAGAGVVADSVPENEHQECIS
                     KAKALFRAAEEAVRFAGRAGRAQ"
     gene            1245..1817
                     /locus_tag="AC16A0_10295"
     CDS             1245..1817
                     /locus_tag="AC16A0_10295"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3105769.1"
                     /translation="MIVLIDNYDSFTWNLYHFLGDLGAETVVHRNDKISVGQVMSAKP
                     EAIVISPGPATPNEAGICLELIDKAAGTIPIFGVCLGLQSMGQAFGGKIVRAPTLMHG
                     KVSQVHHTGKGVFKGIAQDFAATRYHSLIVERDSLPDCLEVTAETDGLIMGLQHKSKN
                     VHGVQFHPESIASEHGHAILRNFLELARTG"
     gene            1817..>2132
                     /locus_tag="AC16A0_10300"
     CDS             1817..>2132
                     /locus_tag="AC16A0_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3105770.1"
                     /translation="MADLKPILAKAASAAPLTRAEAREAFNVMMSGEATPSQIGGLLM
                     ALRVRGETVDEITGAVEIMREKMLRVAAPADSIDIVGTGGDASGSYNISTCAAFVAAG
                     AGL"
BASE COUNT          397 a          674 c          671 g          390 t
ORIGIN      
        1 ggacccttcg agacgattgc tcttccgccg ttgcaggcgc ttcgcgccct gcttgccgag
       61 agccggatca agttgccgga ggatgcgcca ccgatggcgg ctggcgtctt cggctatatg
      121 ggctacgaca tggtccggct catggaggat ctgcctccgc ccaatccaga cacgctgggt
      181 cttcccgacg ccgtgctgat tcgccccacc atcatggcga tcttcgacag cgtccgcgat
      241 gaagtcactg tcacctcccc ggtctacccg agcattgcgg tatcctcccg cgccgcttat
      301 gccagggcgc aggagcggat cagcgaaatc gtcgatgccc tggaccggcc actcgaccat
      361 atcgcgctgc gcgacgagga tatcgcggcg atcagcccgc cgcgatcgaa cacggccccg
      421 gaggactaca aggcgatggt cctgaaggcg aaggaataca ttgcggcggg tgacatcttc
      481 caggtcgtcc tgtcccaacg cttcgagacg gaattcagcc tgccgccctt ctcgctctac
      541 cggtcgctgc gccgggtcaa tccctcgccc ttcctctact acctcgattt cgaccggttt
      601 gccgtgatcg ggtccagccc cgagatcctg gtgcgggtgc gggacggcaa ggtttcgatc
      661 cgtcccattg cgggcacccg tcgccgcggc gcgacgcccg ccgaggaccg tgccctcgcg
      721 gccgaactgc ttgccgatcc aaaggagctg gcggagcatc tcatgttgct cgacctcggc
      781 cgcaacgatg tgggccgggt ggcggagatc ggtaccatca aggtgacgga caagttcatt
      841 ctggagtact acagccaggt gatgcacatc gtatccaatg tcgagggcaa gctggcgcag
      901 aagcatgacg cgatcgatgc gctggtggcc ggcttccctg ccggtaccgt ctccggcgcg
      961 cccaaggtcc gtgccatggc gatcatcgac gagctggaga ccagcaagcg cggcgtctat
     1021 gccggctgcg tcggttactt cagtgccgac ggcgagatgg atacctgcat cgtgctgcgc
     1081 accgccatcg tgaaggatgg aaaaatgtat gttcaggccg gcgccggcgt ggtggcagac
     1141 agcgtgccgg agaacgaaca tcaggaatgc atcagcaagg ccaaggccct gttcagggca
     1201 gcggaagaag ccgtgcgctt cgccggccgc gccgggaggg cgcaatgatc gtcctcatcg
     1261 acaactacga cagctttacc tggaacctct atcacttcct tggagacttg ggggccgaaa
     1321 cggtggtcca ccgcaatgac aagatcagtg tcggacaggt gatgtcagcc aagcccgagg
     1381 ccatcgtgat ctcgcccggg ccagcgaccc ccaacgaggc agggatctgc ctcgaattga
     1441 tcgacaaggc tgctggcacg atcccgatct tcggtgtctg tctcggtctg caatccatgg
     1501 gacaggcctt tggcggcaag attgtgcgcg cgcccacact catgcatgga aaggtctcgc
     1561 aggtgcatca cacaggcaag ggcgtgttca agggaattgc ccaggatttt gccgcaacgc
     1621 gttatcactc cctgatcgtc gagcgagaca gtcttccaga ctgccttgag gtgaccgccg
     1681 agacggatgg cctgatcatg gggctgcaac acaagtccaa gaacgtccat ggcgtgcagt
     1741 tccacccaga gagcatcgcc tccgaacacg ggcatgcgat cctcaggaac ttcctcgaac
     1801 tggcgaggac tggctgatgg ccgacctgaa gccaatcctt gccaaggcag caagtgccgc
     1861 gccgctcacg cgggcggaag cgcgcgaggc cttcaatgtc atgatgtcgg gcgaagcgac
     1921 gccgtctcag atcggcggcc tgctcatggc cttacgggtg cgcggcgaga cggtcgacga
     1981 gatcaccggc gccgtggaga tcatgcggga gaagatgctc cgggtcgccg cccctgctga
     2041 ttccatcgat atcgtcggta cgggcgggga tgcttcgggg tcctataaca tctccacctg
     2101 cgcagccttt gtcgctgccg gtgcggggct ca
//
LOCUS       DBNGVR010000492         3499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4702244, whole genome shotgun sequence.
ACCESSION   DBNGVR010000492 DBNGVR010000000
VERSION     DBNGVR010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4702244"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..2875
                     /locus_tag="AC16A0_10305"
     CDS             233..2875
                     /locus_tag="AC16A0_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3105771.1"
                     /translation="MPDDTDRRTRPASRNLAGWAAMAVFSAVVMVTCLLAALSLNSEV
                     ISRYQGLVTTARVFASSAAEGLSAGDREAQLQALRNVKRLPEIVYAALLDGSGKTIKA
                     LGDPDAAFAGDAKVFDVLTYGTVPVTVGLQSSGGLIGRAQIVADATDLRDHFALSLAA
                     GVSAGMGASLLTYVIFFFLRRRQSARLRQIAEAMDQLAASRSDEILLDPRRAGELAPL
                     FERFNALARSLCAREAAQASRAAEMESATLRLTADLAAARSERADADQAKSDLLAAIG
                     HEIRMPLSGITAAASLLANADLGERHRRHAQVIANSGQSLLAVVNDMLDLSRMVSGGL
                     PLERLPLDPAAIADDVVGLFWDSASAKGLDLAMRAAPGLPMSVTGDPLRLNQILSTLV
                     SQAINCTDRGQILLTLRYEEPHLLCSVSDSGRGLTPLEQSRVFEALPVTEPAGPRATA
                     GAGLGLSIGKRLAQAMGGDITVHSEAGKGSHFTLRIPAPLLLPARPSPRRNTRLERAG
                     LAFRGVASLSALGTSLVASGYEVEVVTDGSADHDLIFVEAGQLAGLPGKRPKAAYVVC
                     LCRADDERGQAARAAGTVQDVLTLPFRQSEILDLLGRLEQAGAAVLQDTPSRPVTTPR
                     FAGRRVLVADDDGINRDVIATALRQLGIAVEAVADGRAAVEAWRRGRPDLILMDCSMP
                     EMDGFAATREIRAREALDIAGGHTPIIALTAIVAGNDAGTWRHAGMDAFMTKPFTLKT
                     ITGCLEAQFAALPPPLQAEAPPPGAAQAVLDSATINDLRRIGGSEALFRRVLDLFAGR
                     VPQAVDKLQAMGSAEDREALADAAHALKSMCANIGALRAVSACDDLELAARTQADFDL
                     GTKLSAIVREVRLTLNEVERLRAA"
     gene            complement(3001..3357)
                     /locus_tag="AC16A0_10310"
     CDS             complement(3001..3357)
                     /locus_tag="AC16A0_10310"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR3105772.1"
                     /translation="MRRLFVALILGCVVGFAGLAGTAEAKTIIGYASYYKHGKRTANG
                     EPFNPNGLTAAHRTLPFGTKVKVTNLKTGKAVIVRINDRGPFIRNRVIDLSFGAAKAV
                     GLTRSGVAKVEVVVLD"
BASE COUNT          552 a         1208 c         1171 g          568 t
ORIGIN      
        1 cgaatccgat ttcggcgagc tgctcgcaca tgacctcgcg catgatcggg tcgtcgtcga
       61 ccaccagcac atgcctggcc cgctgctctc cggccggctg tcgccccgtg ccgctgtcca
      121 ttcgccatcc ttccaaaagc gcaaggagct ttgcagaaag ctgttaaagt ttgcttatgg
      181 gcaaggacag aaacgattct taaatggtcc cgaccgatag tccggcagga tgatgccgga
      241 cgatacagat cgcaggacaa ggccggccag ccgcaatctt gcgggctggg cggcgatggc
      301 ggtgttctcg gccgtggtga tggtcacgtg cctgctcgcc gcgctcagcc ttaacagcga
      361 agtgatatct cgctaccaag gtttggttac tacagctcgt gtgtttgcat cctcggcggc
      421 cgaaggtctc tcggctggcg accgcgaagc ccagctgcag gcgctgcgca acgtcaagcg
      481 cctgcccgag atcgtctatg ccgccctgct cgacggcagc ggcaagacca tcaaggccct
      541 gggcgatccc gatgccgcct tcgcggggga tgccaaggtc ttcgacgtgc tgacctatgg
      601 cacggtgccg gtcaccgtcg ggctgcaatc gtcgggcggg ctgatcggcc gcgcccagat
      661 cgtggccgat gccaccgacc tgcgcgacca tttcgccctc tcgctggccg ctggcgtgag
      721 cgccgggatg ggcgccagcc tgctcaccta cgtcatcttt ttcttcctgc gccgccgcca
      781 gtccgcccgt ctccgccaga tcgcggaggc catggatcag ctcgccgcca gccgcagcga
      841 cgagatcctg ctggatccgc gccgggccgg cgaactggcg ccgctgttcg agcgcttcaa
      901 tgcactggcc cgcagcctct gcgcccgcga ggcggcccag gccagccgtg ccgccgagat
      961 ggaatcggca acgctgcgcc tgaccgccga tctggcagcc gcgcggtccg aaagagccga
     1021 tgccgatcaa gccaagtcgg atctgctggc ggcgatcggc catgagatca ggatgcccct
     1081 gtcggggatc acggctgcgg caagcctgct ggcaaacgcg gatctcggcg agcgccaccg
     1141 ccgccatgcc caggtgatcg ccaattccgg gcagagcctg ctggcggtgg tcaacgacat
     1201 gctcgacctg tcgcgcatgg tgagcggcgg actgccgctg gagcgcctgc cgctcgaccc
     1261 cgcggccatt gccgatgacg tggtgggcct gttctgggac agcgcaagcg caaagggtct
     1321 cgacctcgcc atgcgggccg cgcccggcct gccgatgagc gtgaccggcg atccgctgcg
     1381 cctcaaccag atcctgtcga ccctcgtctc gcaggccatc aattgcaccg acagggggca
     1441 gatcctgctg accctgcgct atgaagaacc gcatctcctg tgcagcgtca gcgacagcgg
     1501 ccgcgggctg acgcccctgg aacagtcacg cgtcttcgag gccctgccgg tcacggaacc
     1561 tgcaggcccg cgcgcaacgg cgggagccgg gctgggcctc tcgatcggca agcgcctggc
     1621 ccaggcgatg ggcggcgaca tcaccgtcca tagcgaagcg ggaaagggat cgcatttcac
     1681 cctgcgcatt cccgcgccgc ttctcctgcc ggcacggccg agcccgcgcc ggaacacgcg
     1741 gctggagcgc gccggtctcg ccttccgggg cgttgcatcg ctgtcggctc tcggtacgtc
     1801 gctggtggcg tctggctacg aggttgaggt ggtcaccgac ggatcggcgg accatgacct
     1861 gatcttcgtc gaagcgggac agctcgcggg tctgcctggc aagcggccga aggccgcgta
     1921 tgtcgtctgc ctctgccgcg cggacgacga gcgcggccag gccgcgcgcg ccgcgggcac
     1981 cgttcaggat gtcctgaccc tgcccttccg ccagtccgag atcctcgacc tcctgggccg
     2041 gctggagcag gccggcgcgg ccgtgctgca ggacacgccg tcccggccag tgacgacgcc
     2101 gcgctttgcc gggcgccgcg tgctggtggc cgacgacgac gggatcaacc gcgacgtgat
     2161 cgccacggcc ctgcgccagc tcggcatcgc ggtcgaggcg gtggccgatg gccgcgccgc
     2221 ggtcgaggcc tggcgccgcg gccgtccgga cctcatcctc atggactgca gcatgccgga
     2281 gatggacggc tttgccgcga cccgcgagat ccgcgcccgc gaggcgctcg acattgccgg
     2341 cggccatacg ccgatcatcg cgctgacggc catcgttgcc ggcaatgatg ccggcacttg
     2401 gcgccatgcc ggaatggacg ccttcatgac caagcccttc acgctgaaga ccatcacggg
     2461 ttgcctggag gcgcagttcg ccgcacttcc gccgccgctg caggccgaag cgccgccgcc
     2521 gggagcggcc caggcggtgc tggactccgc cacgatcaac gacctgcgcc ggatcggcgg
     2581 cagcgaggcg ctgttccgcc gggttctgga tcttttcgcc ggacgcgtgc cccaggcggt
     2641 cgacaagctg caggccatgg gcagtgccga agaccgcgag gcgctggcgg atgcggccca
     2701 tgcgctgaaa tccatgtgtg cgaacatcgg cgccctgcgc gccgtctcgg cctgcgacga
     2761 tctcgaactg gccgcccgga cccaggccga cttcgacctc ggcacgaagc tctcggccat
     2821 cgtgcgggaa gtgcgcctga cgctgaacga agtcgaacgc ctgcgcgcgg cgtgacggcc
     2881 ggcaccggtg ctgcgcaccg gccctcatcc ggccttcggc caccttctcc cgcaggcggg
     2941 agaagggtct tgcgtggatc atccctctcc cgtccgcaga agggcgaagc agaacagaga
     3001 tcagtccagg accacgacct cgaccttggc aacacccgag cgggtgaggc ccacggcctt
     3061 ggccgcgccg aagctgaggt cgatgacgcg gttgcggatg aagggtccgc gatcgttgat
     3121 gcgtacgatc accgccttgc cggtcttgag attggtgacc ttcaccttgg tgccgaacgg
     3181 cagggtacgg tgcgcggcgg tcagtccgtt cggattgaag ggctcgccat tggcggtccg
     3241 cttgccgtgc ttgtagtagg aggcatagcc gatgatggtt ttggcttcag cggtaccggc
     3301 cagacccgca aaaccgacga cacagcccag gatcagggcc acaaacaaac gccgcattgg
     3361 aaacaaaccc cttgagttgc cggctggccc ggctgcgggc acgtggcgcc gcaggaccgg
     3421 aggatgccgg attgcttttg ttgttcgcgg tgaacagcca ttctctgacc atcaggtcaa
     3481 aattgctgca cccgcgaag
//
LOCUS       DBNGVR010000493         6976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5291422, whole genome shotgun sequence.
ACCESSION   DBNGVR010000493 DBNGVR010000000
VERSION     DBNGVR010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5291422"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="AC16A0_10315"
     CDS             <1..1116
                     /locus_tag="AC16A0_10315"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR3105773.1"
                     /translation="RRYYEQDDESVLPRKVACFEAFENAMTLDIAMGGSTNTVLHILA
                     AAHEAGIDFTMKDIDRLSRRVPVLCKVAPAKSDVHMEDVHRAGGIMSILGELDKAGLI
                     NHELPTVHSRTLGEALDRWDIGRTKSESVREFYRAAPGGVPTQVAFSQSRRWDDLDLD
                     RETGVIRSATHPFSKDGGLAVLYGNLAEEGCIVKTAGVDDSILVFTGKARVFESQDAA
                     VLGILNGGVAAGDVVVIRYEGPRGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGG
                     TSGLSIGHVSPEAAEGGLIGLVEEGDPIEIDIPKRSIRLAVSGDVLAKRRAAMQARGD
                     KAWKPAEQRKRNVTTALRAYAAMTTSAAKGAVRVVPE"
     gene            1128..1913
                     /locus_tag="AC16A0_10320"
     CDS             1128..1913
                     /locus_tag="AC16A0_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR3105774.1"
                     /translation="MSIRTVVLGENIHERTNAVVGKLYPKGMHEAIADLLRQDAGIDV
                     STATMEQPEHGLPQSRLEATDVLLWWGHKDHGGVSDDAVERVAKRVWEGMGLIVLHSA
                     HFSKIFRRLMGSPCALKWREAGERERLWVTAPGHPIARGLPQYFELENEEMYGEHFSV
                     PEPLETVFISWFQGGEVFRSGLTYRRGAGNIFYFRPGHETYPTYHDATVGLVLRNAVN
                     WAASRQPAYTAIHEAPNVPVGETLEPVVERGAKLHQPGEQGYR"
     gene            1913..2956
                     /locus_tag="AC16A0_10325"
     CDS             1913..2956
                     /locus_tag="AC16A0_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003561895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3105775.1"
                     /translation="MQLLILGTGCMATSHAMAFAAIPGVRLAGAVDVDKARAEAFAAT
                     YKIPRVFTSLDDAIAWGAFDAAANVTPDSAHHPTTLALIRAGKHVFCEKPLAENHAKA
                     LEMTEAAERAGLVNMVNLTYRNVAALQKARTMVMAGEIGEVRHVDASYLQSWLVSKLW
                     GDWQTESKWLWRLSTAHGSNGALGDIGIHIVDFAAYGAGQDFDRVFCRLKTFDKAPGN
                     RIGEYVLDANDGFAMSVSFVNGALGVIHASRYATGHINELRLRVHGTAGAVEVIHRMD
                     GSSLRACLGADVRNGDWAEVACAPVETNYQRFARAVAAGQALEPSFRRAADMQKVLDQ
                     AMASAAAHRDLMV"
     gene            2966..3604
                     /locus_tag="AC16A0_10330"
     CDS             2966..3604
                     /locus_tag="AC16A0_10330"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR3105776.1"
                     /translation="MPLPKRLLYGYASFRSGRYASEAARYERLGQGSQKPSMMIIACC
                     DSRAAPETIFDAGPGEIFVVRNVANLVPPYMPDGGHHATSAALEFAVLALSVKHVVVM
                     GHGRCGGIHAAVQDTDPLTHTDFIGSWMRGIKDVARYVTHDHSESEELFERRIERASV
                     EHSLANMRTFPWIRMRENAGQLSIHGAWFDISLGELHAYDETAKAWSAIDGN"
     gene            complement(3630..4262)
                     /locus_tag="AC16A0_10335"
     CDS             complement(3630..4262)
                     /locus_tag="AC16A0_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3105777.1"
                     /translation="MRAAATRRRPDTRERILGAAQDAVLEKGFGATSIDELIAAVGIT
                     KSGFFYHFRDKSDLAKALLERYVDDENRILDELFSRAEELHDDPLHQFLIALKLLAEL
                     MEDLPNGHPGCMIASVCYQEQLFNRDVVELNATAVRGWRRRFRQKLDTIAEEYPPQIQ
                     VDLDALADMASGLVDGGIILSKVLRDHRLLPKQVLLYREFIRAIFQGTGR"
     gene            4503..4682
                     /locus_tag="AC16A0_10340"
     CDS             4503..4682
                     /locus_tag="AC16A0_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105778.1"
                     /translation="MMSLKFEAVARRVQQWRSARTTKNATAHLDDRMRRDAGLPVTVT
                     DRRGNRVPLYPFLNG"
     gene            4705..6021
                     /locus_tag="AC16A0_10345"
     CDS             4705..6021
                     /locus_tag="AC16A0_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3105779.1"
                     /translation="MTQLDLQGNHLTGASAAAASAYGDALRQLSIYAGDPLAVADRLV
                     EDEPGFGMAHVLKAWLFLLGTDAKAAAAAREVIVKAEALDLDSREQGHIAAINHLIEG
                     RFHAASRVLEGVAAEHPRDLLALIAGHQLDFFTGSSQLLRDRILRAMPAWSNSQPGYH
                     AMLGMRAFGLEEMGDYAGAEASGRAALEIERCDGWARHAVAHVMEMQGRQDEGIAFMR
                     EDIDSWTAGSFFAVHNWWHLALYHLELGQVDEVLKLVDGPILNAPQEQMLDLVDASAM
                     LWRLQLRDVELGRRWTDLARRYEALWVPGYYAFNDLHAAMAFLGAGQRDQIERIIAAQ
                     GAAEADNLMFSQHVGLPLIRAFVAFHEGRYREAIPLLQSVRGISARFGGSHAQRDIIE
                     LTLIEAAIRGGEAELAQSLGRNRLQTRHDSPLAQLLARRAGLNLAA"
     gene            6100..>6976
                     /locus_tag="AC16A0_10350"
     CDS             6100..>6976
                     /locus_tag="AC16A0_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3105780.1"
                     /translation="MITGDILDWARAAPDKTALIHNGRTLTYRDLANRSLAMIGYLQT
                     QELQAGSTAVIVVRSLLNSWIAIFALRALGVNTITAESLLQMRQLGMQGSAYVIVDSS
                     EKEGGADRSQMAPGQRLIDMSQVPSTLPEAVRAELAGPCGGHILYTSGTTGKFKKVFY
                     EGDKSERVCDHVADRFELTQDTVFHALFFPLYSAAGYFYPVATWRKGGCVVLDQTADA
                     IRRALAYDITMLFASPNILMEIVHEAVVPPERQGTFTICVTGGFLSARLAQRIRDSMT
                     RRIKYYYGATECIDIL"
BASE COUNT         1291 a         2160 c         2205 g         1320 t
ORIGIN      
        1 cggcgctatt acgagcagga cgacgagagc gtgctgccgc gcaaggtcgc ctgtttcgag
       61 gccttcgaga atgccatgac gctcgacatc gccatgggcg gatcgaccaa cacggtcctg
      121 cacatccttg ccgcggccca tgaggcgggc atcgacttca ccatgaagga catcgaccgc
      181 ttgtcgcgcc gcgtaccggt gctgtgcaag gtggcgccag ccaagtccga cgttcacatg
      241 gaagacgtgc accgcgccgg cggcatcatg tcgatcctcg gcgaactcga caaggcgggc
      301 ctcatcaatc atgaacttcc caccgtgcac agccgcaccc tgggcgaagc gctggaccga
      361 tgggatatcg gccggaccaa gagcgagtcg gtgcgcgaat tctaccgggc cgcgcctggc
      421 ggcgtgccca cccaggtggc cttcagtcag tcgcggcgct gggacgacct cgacctcgac
      481 cgcgagacgg gcgtgatccg cagtgccacg catcccttct cgaaggatgg cgggcttgcg
      541 gtgctctacg gcaatctcgc ggaggaaggc tgcatcgtga agacggcggg cgtcgatgac
      601 agcattctcg tcttcaccgg caaggcccgc gtcttcgaga gccaggatgc tgcggtgctg
      661 ggcatcctca acggcggcgt cgccgctggc gacgtggtgg tgatccgcta cgaaggtccg
      721 cgcggcgggc ctggcatgca ggaaatgcta tatcccacca gctatctgaa gtcgaagggc
      781 ctgggcaagg cctgtgctct catcaccgac ggccgtttct cgggcggcac ctcggggctg
      841 tcgatcggtc atgtctcgcc ggaagcggcc gaaggcggtc tcatcgggct ggtggaggag
      901 ggcgatccga tcgagatcga tattccgaag cgatccatcc gcctggctgt gtccggtgac
      961 gtgctggcga agcgccgcgc cgccatgcag gccaggggcg acaaggcctg gaagcctgcc
     1021 gagcaacgca agcgcaatgt cacaaccgcg ctgcgcgcct atgcggcgat gacgaccagc
     1081 gccgccaagg gtgcagtccg cgtcgttccc gaataaggag acaggcaatg tcgatccgca
     1141 ccgtggtttt gggcgagaat atccacgagc gtaccaacgc ggtcgtgggc aaactctatc
     1201 cgaaaggcat gcacgaggcg attgccgacc tcctccgtca ggacgccggg atcgacgtgt
     1261 cgaccgccac catggagcag ccggaacacg gactgccgca gtcccggctt gaggccacag
     1321 acgtgctgct gtggtggggc cacaaggacc acggcggagt ctccgacgac gccgtcgagc
     1381 gcgtggcgaa gcgtgtctgg gagggcatgg gcctcatcgt gctgcactcg gctcatttct
     1441 cgaagatctt ccggcggctc atgggcagcc cctgcgcgct caagtggcgc gaggcgggcg
     1501 agcgcgaaag gctctgggtc acggcacccg gccatcccat cgcgcgcggc ctgccgcagt
     1561 atttcgaact cgagaacgag gagatgtatg gcgaacactt ctcggttccc gagccgctcg
     1621 agacagtctt catctcctgg ttccagggtg gcgaggtttt ccgctccggt ctcacctatc
     1681 gccgcggtgc cggcaacatc ttctatttcc gtcctggtca cgaaacctat ccgacctacc
     1741 atgatgcgac cgtcgggctc gtgctgcgca atgcggtgaa ctgggcggcc agccgccagc
     1801 cggcctatac cgccatccat gaggcgccca atgtgccggt gggcgagacg cttgaaccgg
     1861 ttgtcgaacg cggcgccaag ctgcatcagc ccggcgagca gggttaccgc tgatgcagct
     1921 tctgattctc ggcaccggct gcatggccac ttcacatgcc atggcctttg ctgcaatccc
     1981 cggtgttcgt ctcgccggcg cggtcgacgt cgacaaggca agggcagagg cctttgccgc
     2041 gacctacaag atcccgaggg tttttacttc gctcgatgat gccattgcct ggggagcgtt
     2101 cgacgcagcc gccaatgtca cgcccgacag cgcgcatcat ccaacaacgc tggcgctcat
     2161 ccgagcgggc aagcatgtct tctgcgagaa gccgctggcg gaaaatcatg ccaaggccct
     2221 ggagatgacc gaggccgccg agcgtgcggg actcgtcaac atggtcaacc tgacctaccg
     2281 caacgtggcg gccctgcaga aggcccgcac catggtgatg gcgggcgaga tcggggaagt
     2341 tcgccacgtc gatgcatcct acctgcagag ctggcttgtg tcgaagctgt ggggcgattg
     2401 gcagacggag tcgaaatggc tgtggcggct gtccaccgcc catggttcga acggcgcgct
     2461 gggcgatatc ggcattcaca tcgtcgactt cgcggcttat ggagcgggcc aggatttcga
     2521 tcgcgtcttc tgccgtctca agaccttcga caaggcgccg ggcaatcgca ttggcgaata
     2581 tgtgctggat gccaatgatg gcttcgccat gtcggtgagt ttcgtcaatg gcgcgctggg
     2641 cgtcattcat gccagccgct acgccacggg tcacatcaac gaattgcggc tgcgcgttca
     2701 cggaacggcg ggtgcggtcg aagtcattca ccggatggac ggctcctcgc ttcgcgcctg
     2761 cctcggtgcg gatgtcagga atggcgactg ggcggaagtg gcctgcgcgc cggtcgagac
     2821 caattaccag cgcttcgcca gggccgtggc cgccgggcaa gctctggaac cttcattccg
     2881 ccgtgccgca gacatgcaaa aggtgcttga ccaggccatg gcttcggctg ctgcgcaccg
     2941 cgatttgatg gtttaaggat acgggatgcc tctgcccaag cgcctcctct atggttatgc
     3001 cagcttccgc agcggacgtt atgcttcgga agccgcgcgc tatgagaggc tgggccaagg
     3061 cagtcagaag ccaagcatga tgatcatcgc ctgctgcgac tcgcgtgcgg ccccggaaac
     3121 catctttgat gccgggccag gcgaaatctt cgtcgtacga aacgtagcca atctggttcc
     3181 gccctacatg cccgatggcg gccatcacgc gacaagcgca gcgctcgaat tcgccgtgct
     3241 tgcccttagc gtcaagcacg tcgtggtcat gggccatggc cgctgcggcg gcatccatgc
     3301 ggcggttcag gataccgatc ctctcaccca cacggatttc atcggctcct ggatgcgcgg
     3361 aatcaaggat gtggcgcgct acgtgaccca tgatcactcc gagagcgaag agctcttcga
     3421 gcgccgcatc gagcgcgctt cggtcgaaca ctcactggcc aacatgcgca ccttcccctg
     3481 gatccgcatg cgcgagaatg ccggccagct ctccattcat ggcgcctggt tcgacatttc
     3541 gctgggcgag ctgcatgcct atgacgagac ggcgaaggcg tggtcggcga ttgacggaaa
     3601 ctgatctggc gcaattgcag gctgagggct tatcttcccg ttccctggaa gatcgcgcgg
     3661 atgaattcgc gatagagcag aacctgcttg ggaagcaggc gatgatcccg caacactttt
     3721 gacaggatga tgccgccatc gacgaggccg gaggccatgt ctgcgagtgc atcaagatcg
     3781 acttgtatct gcggcggata ctcttccgca atcgtatcaa gcttctggcg aaaccgcctc
     3841 cgccagccgc ggacagctgt tgcgttgagt tccaccacgt cgcggttgaa cagttgctcc
     3901 tgatagcaaa ccgacgcaat catgcaaccc ggatggccat ttggcagatc ttccatcagt
     3961 tcggccagca gcttgagtgc gatgaggaac tgatgcagcg gatcgtcatg cagttcctcg
     4021 gcgcggctga acagctcatc gaggatgcgg ttctcgtcat cgacatatcg ctcgagcaag
     4081 gccttggcga gatcgctctt gtcacgaaaa tggtagaaga agccgctctt ggtaatgccg
     4141 acggcagcga tcagctcgtc gatggaagtt gcgccaaatc ccttctccag aaccgcatcc
     4201 tgcgcggctc caagaatcct ctcgcgcgta tcggggcggc ggcgggtcgc agctgctctc
     4261 atacaactac tataccacag gtacagttga gcctccactt gtgatgcgga tcaccatcgc
     4321 cgggttcgaa tctgtgccag taagcctccg aaaacactga ctgtttgaga aactttgcgg
     4381 gactgaacca caagccttct ggcgcggcgg ttcgtgtggc ggataggttg ggggcattcg
     4441 caatcgagtg ccgcgtgact gtggctttgc ccaatctggc ggcttgctat ccgatggagg
     4501 acatgatgag tttgaaattc gaagctgtcg cacggcgggt ccaacaatgg cgctccgccc
     4561 gaaccacaaa gaacgccacg gcccatctcg acgaccgcat gcgccgcgat gccggcctgc
     4621 cggtgactgt cacggaccgc cgcggcaacc gcgtgcctct ctatccattt ctcaacggct
     4681 aactgtttca gggagtgcat tcagatgacg caactcgatc tgcagggcaa tcatctcacc
     4741 ggcgccagcg ctgctgcggc atcagcctat ggcgatgcgc tgcgtcagct ctcgatctat
     4801 gcgggcgacc cgctggccgt cgccgacagg cttgtcgaag acgagcccgg ctttggcatg
     4861 gcccatgttc tcaaggcctg gctgttcctg ctcggaaccg acgccaaggc tgccgccgct
     4921 gcgcgcgagg tgattgtaaa ggccgaggcg ctggatctcg acagccgcga acagggtcac
     4981 atcgcagcga tcaatcatct gatcgaaggc cggtttcatg cggcgtcgcg ggttctggaa
     5041 ggcgtcgcgg ccgaacatcc gcgcgatctg ctggcactca ttgcaggcca ccagctggat
     5101 ttcttcaccg gcagttcgca actgttgcgc gaccgcatcc tgcgcgccat gccggcctgg
     5161 tcgaattccc aacccggcta tcatgccatg ctgggcatgc gggccttcgg acttgaggaa
     5221 atgggcgact acgccggcgc cgaggcgagc gggcgcgcgg cactcgaaat cgagcgttgc
     5281 gatggctggg cccggcatgc cgtggcgcat gtcatggaga tgcagggccg gcaggacgaa
     5341 ggcatcgctt tcatgcgcga agacatcgat agctggacgg ccggcagttt ctttgcggtt
     5401 cacaactggt ggcatctcgc gctctatcac ctggagcttg gccaggtgga cgaggtgctc
     5461 aagctggtcg acggtcccat cctgaatgcg ccgcaggaac agatgctcga tcttgtcgac
     5521 gcgtccgcca tgctctggcg actgcaattg cgcgatgtcg aacttggtcg gcgctggacg
     5581 gatctggctc ggcgctatga ggctctctgg gtgcccggct actacgcctt caatgacctg
     5641 cacgccgcca tggcctttct aggcgctggg caacgtgatc agatcgagcg catcattgcc
     5701 gcgcagggag ctgcggaggc ggacaacctc atgttctcgc agcacgtggg gcttcccttg
     5761 atcagggcct tcgtggcctt tcatgaaggc cgttatcgcg aagccattcc gctcctgcag
     5821 tcggtgcgtg gcatctctgc gcgtttcggc ggcagccacg cacagcgtga catcattgaa
     5881 ctgacgctga tcgaggcagc catacgcggt ggcgaagccg agttggcgca gtcgctgggc
     5941 cgcaatcgcc tgcaaacgcg gcatgacagt ccgctcgcgc agctgctggc gcggcgggcc
     6001 gggctcaacc tggcggcctg atcagaagcg atggctggga gaggccttgc ggccactccc
     6061 gtctctgctt tactgggttc cagtcacgaa gtggaactca tgatcaccgg cgacatcctc
     6121 gactgggcac gtgcagcgcc cgacaagacg gccctcattc acaatggccg tacgctgacc
     6181 tatcgcgacc tggccaaccg cagcctggcg atgatcggct accttcagac ccaggaactg
     6241 caagctggca gcacggcggt gattgtggtc cgaagccttc tgaacagctg gatcgccatc
     6301 ttcgcgctgc gggctctggg cgtcaacacg atcaccgctg agtcgctgct gcagatgcgg
     6361 cagcttggca tgcaaggctc cgcctatgtg attgtcgatt cgagcgagaa agagggaggg
     6421 gcagaccgca gccaaatggc gccggggcag aggctgatcg acatgtcgca ggtgccgtca
     6481 accctgcccg aagcggtgcg ggcagagctt gcagggccat gcggcggaca catcctctac
     6541 acttccggaa ccacggggaa gttcaagaag gtcttctatg agggggacaa gtcagaacgc
     6601 gtctgcgatc atgtcgccga tcgctttgaa ttgacccagg ataccgtgtt ccacgccctg
     6661 ttttttccgc tctattcagc cgccggctac ttctacccag tggcaacctg gcggaaaggt
     6721 ggatgcgtgg tgctggatca gacagccgat gccatcagac gggccctggc ctacgacatc
     6781 acgatgctgt tcgcgtcgcc gaacattctc atggaaatcg tccatgaggc cgtcgtgcca
     6841 ccggagcggc agggcacctt cacgatctgc gtgaccggcg gctttctgtc ggcgcgcctt
     6901 gctcagcgga tccgggattc catgacgcgc aggatcaaat actactatgg tgctacggaa
     6961 tgcatcgaca tcctgc
//
LOCUS       DBNGVR010000494         2159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2543334, whole genome shotgun sequence.
ACCESSION   DBNGVR010000494 DBNGVR010000000
VERSION     DBNGVR010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2543334"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /gene="cobM"
                     /locus_tag="AC16A0_10355"
     CDS             complement(<1..618)
                     /gene="cobM"
                     /locus_tag="AC16A0_10355"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR3105781.1"
                     /translation="MTVHFIGAGPGAADLITLRGLKLIQSCPVCLYAGSLVPEEIVKA
                     APEGARVVDTAPLHLDEIIAEMEAAHAKGHDVARVHSGDPSLYGAIAEQMRRLDRLGI
                     PYDVTPGVPAFAAAAAALKTEFTLPEIAQTVILTRTSMQASPMPEGEALEELGRSRAT
                     LAIHLSIRNLAHIERALTPHYGPDCPVVVAYRITWPDQMIIKGTLS"
     gene            complement(615..>2159)
                     /gene="cobJ"
                     /locus_tag="AC16A0_10360"
     CDS             complement(615..>2159)
                     /gene="cobJ"
                     /locus_tag="AC16A0_10360"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIR3105782.1"
                     /translation="VVAVAEDGSSAVPLLGGHHGANDLARRIAAVCKGDAAITTASDL
                     RLGVALDEPAAGYVLGNPEDMKSVTAAILAGATVRLEAAPGLSPFPGLDPAPADGAAP
                     FVTLKVTHARIGGDGQTLVYHPKTLVLGIGCERGVPAKDVIAHVEAVLSSEGLVSQAL
                     AAIASIDVKADEAALHAVARHFDVPARFFTAEELRAEAHRLRNPSALVEKEVGTPGVA
                     EAAALAMVGKGGTLLVEKRKSARATCAVAQASAPLTDLPGQARGILHVVGLGPGTPEW
                     RAPAASAALSSSTDWVGYGLYLDLAADLRDGQTEHRFPLGGEEDRVRHAIALARQGKE
                     VSLICSGDAGIYAMAALVYEIVDLEPARIAVNVIPGISAFQAAAAKAGAMIGHDFCCI
                     SLSDLLTPWEVIERRLKAAAEGDFVIAFYNPRSLKRRDQIERAFAILARHRPASTPVI
                     IASNLGRPEEKVKIVPFAQFDPDDVDMLTLVMVGSSQSKSFARGDGATYAYTPRGYAR
                     KREAAE"
BASE COUNT          354 a          714 c          754 g          337 t
ORIGIN      
        1 cgaaagtgtc cccttgatga tcatctggtc gggccaggtg atgcggtagg cgacgaccac
       61 ggggcagtcc ggcccgtagt gcggcgtcag ggcccgctcg atatgggcga ggttgcggat
      121 ggagagatgg atggcgagcg tggccctgga gcgtcccagt tcctccagcg cctcgccttc
      181 cggcatgggc gaggcctgca tggaggtgcg ggtgaggatg acggtctgtg cgatctcggg
      241 cagcgtgaac tccgttttca gcgccgccgc cgccgcggcg aaggccggca cgcccggcgt
      301 cacgtcatag ggaatgccga ggcggtcaag ccgccgcatc tgctcggcga tggcgccata
      361 gagcgacggg tcgccggaat gcacgcgggc cacatcatga cccttcgcat gcgcagcctc
      421 catctcggcg atgatctcgt cgagatgcag cggcgcggtg tcgaccacgc gcgcaccttc
      481 gggcgcggcc ttcacgattt cttcgggcac cagcgatccg gcatagaggc agaccgggca
      541 ggactggatc agcttcagcc cgcgcagcgt gatcaggtcg gcggcgcccg gtccggcacc
      601 gatgaaatgg acggtcattc tgcggcctct ctcttcctgg catagccgcg cggcgtatag
      661 gcataggttg cgccatcgcc gcgcgcgaag gacttggatt gcgaagaccc gaccatgacc
      721 agcgtgagca tgtcgacgtc gtccggatcg aactgcgcga agggcacgat ctttaccttt
      781 tcctcggggc ggccaaggtt ggatgcgatg atcaccggcg tgctggccgg ccggtggcgt
      841 gcgaggatgg cgaaggcgcg ctcgatctgg tcgcggcgct tcagcgagcg cggattgtag
      901 aaggcgatca cgaagtcgcc ctctgcagcg gccttcagcc gccgttcgat gacctcccag
      961 ggcgtcagca ggtcggacag cgagatacag cagaagtcgt ggccgatcat ggcgcctgcc
     1021 ttggccgcgg cggcctggaa ggcggagatg ccgggaatga cattgaccgc gatgcgcgcg
     1081 ggctcgagat caacgatctc gtagaccagc gctgccatgg catagatgcc cgcatcgccg
     1141 gaacagatga gcgatacctc cttgccctgc ctggcaagcg cgatggcatg gcgcacgcgg
     1201 tcttcctcgc cgcccagcgg aaagcggtgc tcggtctggc cgtcgcgcag gtcggcggca
     1261 agatcgagat agagaccgta gcccacccag tcggtggaag atgacagagc ggcgctcgcg
     1321 gccggcgccc gccactccgg cgtgccgggg ccgaggccaa cgacatgcag gatgccgcgc
     1381 gcttgtcccg gcaggtcggt cagcggcgca gaggcctggg ccacggcgca ggtcgcgcgc
     1441 gccgacttcc gcttctccac cagaagcgtc ccgcccttgc cgaccatcgc caaagccgcg
     1501 gcctcggcca cgcccggcgt tcccacttcc ttctcgacaa gcgcggacgg gttcctgagg
     1561 cgatgggcct cggcgcgcag ctcctcggcg gtgaagaagc gtgcaggaac atcgaaatgt
     1621 cgggccaccg catggagcgc ggcctcgtcg gccttcacat cgatggaggc aatcgccgcc
     1681 agcgcctgcg acacgagtcc ctcggatgac agtacggcct cgacatgggc gatgacatcc
     1741 ttcgccggga cgccgcgctc gcagccgatg ccgagcacga gtgttttcgg atgatagacc
     1801 agggtctgtc catcgcctcc gatccgcgca tgcgtcacct tcagcgtgac gaagggcgcg
     1861 gcgccgtctg cgggcgcagg atcgagaccg ggaaacggtg aaaggcccgg cgcagcctcc
     1921 agcctcaccg tggcgccggc caggatcgcg gccgtgacgc tcttcatgtc ctcgggattg
     1981 cccagcacat agcccgctgc cggttcatcg agcgccacgc cgaggcgcag gtcgctcgcg
     2041 gtggtgatcg ccgcgtcacc cttgcagact gcggcgatgc ggcgtgcgag gtcattggcg
     2101 ccatgatggc cgccgagcag cggcacggcg gaactgccgt cctcggccac cgcaaccac
//
LOCUS       DBNGVR010000495         1608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4309341, whole genome shotgun sequence.
ACCESSION   DBNGVR010000495 DBNGVR010000000
VERSION     DBNGVR010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4309341"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..509)
                     /locus_tag="AC16A0_10365"
     CDS             complement(78..509)
                     /locus_tag="AC16A0_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105783.1"
                     /translation="MRDVVLALGLAAVWTVGTPILDARHQEPAPTVSAPDWLAIPLDA
                     EITEVRRSRAQAAGHLNAVSDLPAAAVVEIMKTRLALQGFEIIDRSADLTGTSGAATA
                     FQAANPTDGRLATAVVVDTPTGAMLRIDFSEQESVSRLSSL"
     gene            complement(595..>1608)
                     /locus_tag="AC16A0_10370"
     CDS             complement(595..>1608)
                     /locus_tag="AC16A0_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3105784.1"
                     /translation="SPAMTDFIFMVRGTSYMFVTGPDVVKTVTNETVTAEELGGASVH
                     TTRSSVADKGFDNDVEALLQMRRLIDFLPSSNTSPIPELPSFDPPDRTDMSLDTLIPA
                     NPNQPYDMKELILKIVDEGDFFEIQDAHARNIITGFTRVNGRTVGIVANQPMVLAGVL
                     DSDAGRKAARFVRFCDCFNIPIVTLVDVPGFLPGTAQEYGGLIKHGAKLLYAYAEATV
                     PKITVITRKAYGGAYVVMASKHLRGDVNYAWPTAEIAVMGSKGAAEILYRAELGDPAK
                     IQARIKEYEQRFANPFVAAERGFLDEVIMPHSTRRRIARALNMLRHKELTNPWKKHDN
                     IPL"
BASE COUNT          304 a          466 c          528 g          310 t
ORIGIN      
        1 gaaaagcgag gtccagcatg ttcatggtgc aagtctagcg ccgatgcccc ggccgcgtct
       61 tgatctggat gaaaccgtca gagactggac aagcgggata cggattcctg ttcggaaaag
      121 tcgatccgca gcatggcgcc cgtcggtgtg tcgaccacaa cggccgtcgc aaggcggcca
      181 tcggtcggat tggcagcctg aaaggcggta gcggctccgg aggttccggt cagatcggcg
      241 gacctgtcga tgatctcgaa tccctgcagc gcaaggcggg ttttcatgat ctcaaccacc
      301 gctgcggcag gcaggtcgga cacggcgttg agatgccctg cggcctgcgc gcgcgaccgg
      361 cgcacctccg tgatctcggc atccagcgga atggcaagcc agtctggtgc cgacacagtg
      421 ggcgccggct cctggtggcg ggcatcgagg atcggcgtgc cgacagtcca gacggcagca
      481 agtcccaggg ccagaacgac atcgcgcatg aatgcacctt ctcggaagca ttcgacacta
      541 cgcgaagttt tctaagctgc agttctacca aatcgtaaaa ttgaaaccat atagtcaaag
      601 tggaatgttg tcgtgcttct tccaaggatt tgtaagttcc ttgtgccgca gcatgttgag
      661 cgcccttgcg atgcggcgcc gcgtcgaatg cggcatgatc acctcgtcga ggaagccgcg
      721 ttctgcggca acgaaggggt tggcgaagcg ctgctcgtat tccttgatcc gcgcctggat
      781 cttggcggga tcgcccagct cggcgcgata gaggatttcg gcagccccct tggaacccat
      841 gacggcgatc tcggcggtgg gccaggcata gttcacgtcc ccgcgcagat gcttggaggc
      901 catgaccaca taggccccgc cataggcctt gcgggtgatg acggtgatct tcggcaccgt
      961 cgcctcggca taggcataga gaagcttggc gccatgcttg atgaggccgc cgtactcctg
     1021 cgcggtgccc ggcaggaagc ccggcacgtc gacaagcgtc acgatcggga tgttgaagca
     1081 gtcgcagaag cgcacgaagc gcgccgcctt gcgtccggca tcgctgtcga gcacgccggc
     1141 cagcaccatc ggctggttgg cgacgatgcc gacagtccgg ccattcaccc gggtgaagcc
     1201 ggtgatgatg ttgcgcgcat gcgcgtcctg aatctcgaag aagtcgccct catcgacgat
     1261 cttcaagatc aattccttca tgtcataggg ttggttggga ttggccggga tcagcgtgtc
     1321 gagcgacatg tcggtgcggt cgggcgggtc gaagcttggc agttccggga tgggagaggt
     1381 gttggacgac gggaggaaat cgatcagccg gcgcatctgc agcagcgcct caacgtcgtt
     1441 gtcgaagccc ttgtcggcca ctgacgaccg ggtggtatga accgaggcgc cgcccagttc
     1501 ttctgccgtc accgtttcgt tggtcacggt cttgaccacg tcggggccgg tcacgaacat
     1561 gtagctggtg ccgcgtacca tgaagatgaa gtcggtcatt gccggcga
//
LOCUS       DBNGVR010000496         1962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_5293070, whole genome shotgun sequence.
ACCESSION   DBNGVR010000496 DBNGVR010000000
VERSION     DBNGVR010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_5293070"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1962)
                     /gene="leuS"
                     /locus_tag="AC16A0_10375"
     CDS             complement(<1..>1962)
                     /gene="leuS"
                     /locus_tag="AC16A0_10375"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3105785.1"
                     /translation="ERKVSMVNWDPVDHTVLANEQVIDGRGWRSGALVEKRELAQWFL
                     KITAYSDELLSALDGLTRWPEKVRLMQKNWIGRSEGLRFSFALETADGKPLDERLTVY
                     TTRHDTIFGASFCAISADHELARRLSEKDEGLAAFRREVAALGTSEETIERAEKKGHA
                     LGLYARHPFRKDVRLPVYAANFVLMGYGEGAIFGCPAHDQRDLDFARKYDLPVLPVVA
                     PKGTDPASFTVGSEAFIEKEGDNTVLINSDFLDGMSVPAAKAQIAKRMEAMGIGERRV
                     NFRLRDWGVSRQRYWGCPIPVIHCAACGIVPVPKDQLPVTLPEDANFDKPGNPLEHHP
                     TWKHVACPSCGKPAKRETDTFDTFIDSSWYYARFCSPHADVPVTPEAARYWMGVDQYI
                     GGVEHAILHLLYSRFYARAMTATGYLDVKEPFESLFTQGMVIHETFRTLSGEWVLPAD
                     AVNQDGKWVHATTGEPLEVGGVEKMSKSKKNVVDPDEIIARFGADTARWFMLSDTPPE
                     RDIEWTEAGAEGAWRFTHRIWRLVNDAAAHAGGSDAAASLELRRAAHKALTAVTEDLG
                     ALRFNRAIARIYELANALGQAMGKAEAPALREAAEFLVLMSAPMMPHLAEECWQALGH
                     TQAVVDTPWPKADPALVADDTVTIAVQVNG"
BASE COUNT          313 a          662 c          649 g          338 t
ORIGIN      
        1 gccattgacc tgcacggcga tggtgaccgt gtcgtccgcc accagagccg ggtccgcctt
       61 gggccagggc gtgtccacca ccgcctgtgt gtggcccagc gcctgccagc attcttcggc
      121 cagatgcggc atcatcggcg ccgacatcag gacgaggaac tccgccgcct cgcgaagcgc
      181 cggagcctcg gccttgccca tggcctggcc cagcgcattg gccagctcgt agatgcgcgc
      241 gatggcgcgg ttgaagcgca gcgccccgag atcctcggtc accgccgtca gcgccttgtg
      301 cgccgcgcgg cgcagctcca ggcttgctgc cgcatcgctg ccgccagcat gcgcggcggc
      361 atcgttgacc aggcgccaga tgcgatgggt gaagcgccag gcgccctcgg caccggcttc
      421 ggtccattcg atgtcgcgct cgggcggcgt gtcggacagc atgaaccagc gcgccgtatc
      481 ggcaccgaag cgggcaatga tctcgtcggg gtcgaccacg ttcttcttcg acttcgacat
      541 cttctcgacg ccgcccactt ccagcggctc gccggtcgtc gcatgcaccc acttgccgtc
      601 ctggttcacg gcatcggccg gcagaaccca ctcgccactc agtgtacgga aggtttcatg
      661 gatgaccatg ccctgggtaa agaggctctc gaagggctcc ttcacgtcga gataaccggt
      721 ggccgtcatg gcccgggcat agaagcggga atagagcaga tgcaggatcg cgtgctcgac
      781 gccgccgatg tactggtcga cgcccatcca gtagcgcgcc gcctccggcg tcaccggcac
      841 atcggcgtgg ggcgagcaga agcgtgcata gtaccaggac gagtcgatga aggtgtcgaa
      901 ggtgtcggtc tcgcgcttcg ccggcttgcc gcaggacgga caggcgacat gcttccaggt
      961 cgggtggtgt tccagcggat tgcccggctt gtcgaagttg gcatcctcgg gcagcgtcac
     1021 cggcagctgg tccttcggca ccggcacgat gccgcaggcg gcacagtgga tcaccggaat
     1081 ggggcagccc cagtagcgct ggcgcgacac gccccagtcg cgcaggcgga aattcacgcg
     1141 gcgctcgccg atgcccatcg cctccatgcg cttcgcgatc tgggccttgg ccgcgggcac
     1201 gctcatcccg tcgaggaagt cggaattgat gagcacggtg ttgtcgcctt ccttctcgat
     1261 gaaggcctcg ctgcccacgg tgaagctcgc gggatcggtg cccttcggcg ccaccaccgg
     1321 caataccggc agatcgtact tgcgggcgaa gtcgaggtcg cgctggtcat gcgcggggca
     1381 gccgaagatg gcgccctcgc catagcccat gagcacgaaa ttcgcggcat agaccggcag
     1441 ccgcacgtcc ttgcggaatg ggtggcgggc atagagtccc agcgcatgac ccttcttctc
     1501 ggcgcgttcg atggtctcct cggaggtgcc gagcgctgcc acctcgcggc ggaaggcggc
     1561 aaggccctcg tctttctccg acaggcggcg cgccagctca tggtcggccg agatggcgca
     1621 gaagctggcg ccgaagatcg tgtcatggcg cgtggtatag acggtcaacc gctcgtccag
     1681 tggtttgcca tcggcggttt ccagcgcgaa ggagaagcgc aggccctcgg agcggccgat
     1741 ccagttcttc tgcatcagcc ggaccttttc cggccagcgc gtgaggccat cgagggccga
     1801 cagcagttcg tccgaatagg cggtgatctt caggaaccac tgggccagtt cgcgcttctc
     1861 gaccaatgcg cccgagcgcc agccgcgccc gtcgatcacc tgctcgttgg ccagcaccgt
     1921 gtggtccacc gggtcccagt tcaccatcga gaccttgcgc tc
//
LOCUS       DBNGVR010000497         6450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1758152, whole genome shotgun sequence.
ACCESSION   DBNGVR010000497 DBNGVR010000000
VERSION     DBNGVR010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1758152"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="AC16A0_10380"
     CDS             <1..374
                     /locus_tag="AC16A0_10380"
                     /inference="COORDINATES: protein motif:HMM:NF052838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3105786.1"
                     /translation="RINFMDVSVNAGTDTVEVRASFPNPERLLVDGQLVTAVVESDKP
                     QPSLVVPQVAIQIDQTGPFVLVVNSENKIEVRRVEPGVSSGMLLVIKKGLQAGDLVVT
                     EGVQKVRPGQAVQATVIMPEA"
     gene            378..3527
                     /locus_tag="AC16A0_10385"
     CDS             378..3527
                     /locus_tag="AC16A0_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3105787.1"
                     /translation="MFSGIFIDRPRLAVVISLVITIAGLVAMQQIPVAQFPNIVPPQV
                     SVTASYAGASAEVVEAAVGQPLESRVVGVDNMIYMKSTSGNDGSYALTITFAVGTNPD
                     LNTVNVQNRVSLAEAQLPQEVRSQGIQVTKKSSAILQLIAFTSSQESHDQLFLSNYAT
                     INVIDRLKRVPGVGDVQLLTPSDYSMRIWVDPQRLADFGMTPADIINALRAQNIQAAI
                     GRIGSQPALPDQRFQLTVQTQGRLSSVEEFGNVVVRADPGGSFVRVRDVARVELAARL
                     SEQQGRQDGSPAAVIGIYQAPGGNAIASAEAVRGLLDEAKNQFPEGMDYKITYDTTVF
                     VAESIHEVIKTLVEAFALVVIVVFIFLGSLRATIIPLIAVPVSLVGTFAVMMAMGYSA
                     NTVSLLALVLAIGIVVDDAIVVVEAVEAKMEADHSLSPAEAARLAMGEITAPIIAIKL
                     VLMSVFVPVAFIPGISGELFRQFAVVVTVSMIISAINALTLSPALCGVLLKSSHGPKR
                     FPMRQIMSGINLARDGYAAVVKRLVRIAILGVVALAVMIPATGWLFKTTPTGFLPSED
                     QGAIFGEVILPEGSSVNLTNEVTRQVEDVVRQTPGVDGIMSVVGYSMLDGQVKSNAAL
                     LVLTLKPFADREDPSLSAEALIGRLSIQGQQIRRANVIFYNLPPIIGLGNGSGFEYQL
                     LSMTGAPAADIAAVARGLVFASNQNPVLQRVFTTFSASTPQLWLDIDRDKVQTLGIQV
                     SDVFSTLQAVLGSAYVNDFNLFGRTWQVVVQGEASDRSKIDDIYRINVRNNKGEMVPV
                     RAFAEASLILGPQSLIRFNNLRSATINGGPARGYSSGEAIAAMERTSAAALPQGYSYE
                     WTGTALQEKEASGKTTIILGLALLFAYLFLVGLYESWTIPIAVLLSVTAGIMGSMLAL
                     KWSGLDNNLYAQIGLVVLIALAAKNGILIVEFAMERRRQGLGIAEAATEGAKERFRAV
                     MMTSFAFIAGLVPLVIAEGAGMLSRRGVGTAVFGGMLAAALVGIFLIPLLYVVFQWMR
                     EKVKGKPRAPATVVEAVTP"
     gene            3524..5791
                     /locus_tag="AC16A0_10390"
     CDS             3524..5791
                     /locus_tag="AC16A0_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HIR3105788.1"
                     /translation="MTTPNRWKRYADWDQRPLRLDRFAAEDAANGFSAFSSPADPKPG
                     LEVSGGKVVSLDGVLEHDFDMIDRFIARHHIDVAMAPEAMAMDSAQLARMLVDMNVPR
                     EDLVRLAHGMTPAKLAEVVSHLNALEIAFAYSKMRARRTPGNQAHVTNAKDDPLQLAA
                     DAATAVAFGFDEIETTMRVSRNAWSNALACAVGGAVGRWGTLFQCSSEEAEELRIGMA
                     GFTSYAETVSVNGTETTFIDGDDTPWSKAFLAAAYASRGIKKRCTSGAGSELLMGFHE
                     SKSLLYLEARCLCLQRGMGVQGTQNGGIDGAPLTATMPGGIRELMAENLIAVWLDLEC
                     ASGNDARSTESEIRVGAKILPYLVAGSDLICSGFGSILKYDNSFNPSLLNGEELEDYL
                     VLQRDFEADGGLTPLPEERALDLRRRAIAAVSAVFEELELAQPTTEMKQSVAVASGSD
                     ETRSFNPRDVSFISEAIKDRGIGVIDVIKALARRGFREEAENLLNVVKLRVSGDYLQT
                     SAMIRDGKVISAVNDPNTYLGPGTGYRVTEERRQQLNAIRDVLDQKEVLRSEAMHEKE
                     EARRIRYRILGAALPGTAPGDIVIGISPAFGLKLFQTTAGHRLSDVLHEIMSAIRARG
                     LTPRVVRFLHTADTSFLGLSAARLAGSGIGIGLQAKGTAVIHSKDRLPHNNLELFSNA
                     PITQLAHYRGLGANAAAYALGEMPEPVVVPQRGEAMGSRYHARVALIYAIETGLTAEG
                     AAPEEIEVTLTGAKP"
     gene            5788..6207
                     /locus_tag="AC16A0_10395"
     CDS             5788..6207
                     /locus_tag="AC16A0_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HIR3105789.1"
                     /translation="MTLSRTDYPLAETQPDRIRGKRGLRLTDITLDAVMAGDITMEDL
                     RITPDALRAQADIARDAGRPTLALNFERAAELVDVPQDFIMQVYELLRPGRARSRQQL
                     LDAAATLRRDHGAERMAGFVEEAAQAYEDRGLFTYRF"
     gene            6217..>6450
                     /locus_tag="AC16A0_10400"
     CDS             6217..>6450
                     /locus_tag="AC16A0_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3105790.1"
                     /translation="MSWKTKYRSLYYMGAPEPDDPVLDPKETALLVIDVQNTYLERPD
                     QSSLSPEEQRRYDLWTPFHDRMRTSVIPNTARML"
BASE COUNT         1135 a         2105 c         2059 g         1151 t
ORIGIN      
        1 gacggatcaa cttcatggac gtgtcggtca atgccggcac cgacacggtc gaagtgcgcg
       61 ccagttttcc caatcccgaa cgcctgctgg tcgatgggca gctggtcacc gccgtggtcg
      121 aatccgacaa gccgcagcct tcgctggtgg tgccgcaggt ggccatccag atcgaccaga
      181 ccggcccctt cgtcctggtg gtgaattcag agaacaagat cgaggtgcgg agggtagagc
      241 ccggagtctc ctcgggcatg ctgctggtta tcaagaaagg actgcaggcc ggcgatctcg
      301 tcgtcaccga aggcgtccag aaagtcaggc caggccaggc cgtacaggcc accgtcatta
      361 tgccggaggc ctgatccatg ttctccggga tcttcatcga ccggccgcgc cttgcggtgg
      421 tcatctcgct ggtcatcacc atcgcgggcc ttgtcgccat gcagcagatc ccggtggcgc
      481 agttccccaa catcgtgccg ccgcaggtct cggtcacggc ctcctatgcc ggcgcaagcg
      541 ccgaagtcgt cgaggctgcc gtaggtcagc cgctcgagtc ccgcgtcgtc ggcgtcgata
      601 acatgatcta catgaagtcg accagcggca atgacggaag ttacgcgctc accatcacat
      661 ttgcggtcgg cacaaatccc gacctcaaca cggtgaacgt gcagaaccgg gtcagcctgg
      721 ccgaggcgca gctgccgcag gaagtgcgca gccaggggat ccaggtgacc aagaagtcat
      781 ccgccatcct gcagctgatc gccttcacct cgagccagga gtcgcatgac cagctcttcc
      841 tctcgaacta tgcgaccatc aacgtcatcg accgcctgaa gcgcgtgccg ggcgtaggcg
      901 acgtgcagct gctgacgccc tcggactaca gcatgcgcat ctgggtcgac ccgcagcgcc
      961 tggccgattt cggcatgaca cctgccgaca tcatcaatgc gctgcgggcg cagaatatcc
     1021 aggccgccat cggacgcatc gggtcccagc cggcgctgcc cgaccagcgc ttccagctga
     1081 ccgtgcagac gcagggccgg ctgagttcgg tggaggaatt cggcaatgtc gtggtgcggg
     1141 ccgatccggg cggctcgttc gttcgcgtgc gcgacgtggc ccgcgtcgag ctggcggcgc
     1201 ggctctccga acagcagggc cgccaggatg gaagtccggc cgcggtgatc ggcatctacc
     1261 aggcgccggg gggcaatgcc atcgccagcg ccgaagccgt gcgcggcttg ctggacgaag
     1321 cgaagaatca gttcccggag ggcatggact acaagatcac ctacgacacg accgtcttcg
     1381 tcgccgaaag cattcacgag gtgatcaaga cgctggtcga ggccttcgcg cttgtcgtca
     1441 tcgtggtctt catcttcctc ggctcgctgc gcgcaaccat cattcccctg atcgccgtgc
     1501 ccgtctctct ggtcggtacc ttcgctgtga tgatggcgat gggatactcc gccaataccg
     1561 tctcgctgct ggcgctggta ctggccatcg gcatcgtcgt cgatgacgcc atcgtggtgg
     1621 tggaggcggt cgaggcgaag atggaggcgg atcattcgct ctcgccggcc gaagccgcgc
     1681 ggcttgccat gggcgagatc accgcgccca tcattgccat caagctggtg ctgatgtcgg
     1741 ttttcgtgcc ggtggccttc atcccgggca tttcgggcga gctgttccgg cagtttgccg
     1801 tggtcgtcac cgtttcgatg atcatctcgg ccatcaacgc cctgacactg tcgcctgcgc
     1861 tctgcggcgt tcttctcaag agcagtcatg ggcccaagcg ctttccgatg cgtcagatca
     1921 tgtccggcat caatctggcg cgcgacggct atgccgccgt ggtcaagcgg ctggtgcgga
     1981 tcgccatcct cggcgtcgtg gcgctggcgg tcatgattcc cgcgaccggc tggctgttca
     2041 agacgacgcc gaccggtttc cttccgtccg aggatcaggg cgccattttc ggcgaagtga
     2101 tcctgcccga gggttcgtcc gtgaatctca ccaacgaagt gacgcggcag gttgaggacg
     2161 tggtccgcca gacgcctggc gtcgatggca tcatgtcggt ggttggctac agcatgctgg
     2221 acggtcaggt gaagtccaat gctgccctgc tggtgctgac gttgaagccc ttcgccgacc
     2281 gcgaggatcc ctcgctctcg gccgaagcgc tgatcggcag gctttccatc cagggccagc
     2341 agatccgccg tgccaacgtg atcttctaca atcttcctcc gatcatcggc ctcggcaatg
     2401 gcagcggctt cgagtatcag ctgctgagca tgaccggcgc gcccgccgcc gacatcgcgg
     2461 cggtagcccg cggcctggtc ttcgcctcca accagaatcc ggtgctgcag cgcgtcttca
     2521 cgaccttctc ggccagcaca ccgcagctct ggctcgacat cgaccgcgac aaggtgcaga
     2581 cgctgggtat ccaggtctcc gatgtcttca gcacgctgca ggcggtgctt ggcagtgcct
     2641 atgtgaatga cttcaacctg tttggccgaa cgtggcaggt ggtcgtgcag ggcgaagcgt
     2701 cggatcgcag caagatcgac gacatctacc ggatcaacgt gcgcaacaac aagggcgaga
     2761 tggtgccggt ccgcgccttc gccgaggcca gcctgatcct cggtccgcag tcgctgatcc
     2821 gcttcaacaa cctccgcagc gccaccatca atggcgggcc agcgcgcggt tacagctccg
     2881 gcgaggcgat tgccgcgatg gaacggacat cggccgccgc gctgcctcag ggctacagct
     2941 acgagtggac cggtaccgcg ctgcaggaga aggaagccag cggcaagacc accatcatcc
     3001 tgggactggc cttgctgttc gcctatctct ttctcgtcgg cctctacgag agctggacca
     3061 tccccatcgc ggtacttctc tctgtgacgg ccggcatcat gggttcgatg ctggcgttga
     3121 aatggtcggg gctcgacaac aatctctacg ctcagatcgg gctcgtggtg ctgatcgcgc
     3181 ttgccgcaaa gaacggcatc ctcatcgtgg aattcgccat ggagcgccgg cgccagggac
     3241 ttggcatcgc cgaggctgcg accgagggcg ccaaggaacg cttccgcgcc gtgatgatga
     3301 caagctttgc cttcatcgca ggccttgtgc cgctggtgat cgccgaaggc gccggcatgc
     3361 tgagccgccg tggcgtcggc accgccgtct tcggcggcat gctggctgcc gcactggtcg
     3421 gcatcttcct catcccgctg ctctatgtcg tcttccagtg gatgcgggag aaggtgaagg
     3481 gaaaacccag ggcacccgcc accgtggtgg aagccgtaac gccatgacga cgcccaaccg
     3541 ctggaagcgc tatgccgact gggatcagcg gccgctgcgg ctcgatcgct tcgcggcgga
     3601 agacgcagcc aacggcttca gcgccttctc cagccccgcc gacccaaagc ccggactgga
     3661 agtgagcggc ggcaaggtcg tctcgctcga cggcgtcctc gaacatgact tcgacatgat
     3721 cgaccgtttc atcgcgcgcc accacatcga cgtggcaatg gcacccgaag ccatggcgat
     3781 ggactccgca cagctggccc gcatgctggt ggacatgaat gtgccgcgcg aggacctcgt
     3841 ccgcctcgcg catggcatga ccccggccaa gctcgccgag gtggtatccc atctcaatgc
     3901 tctcgaaatc gcctttgcct attcgaagat gcgggcgcgc cgcacccccg gcaaccaggc
     3961 gcatgtcaca aacgccaagg acgacccgct gcagctggca gccgacgcgg caacagcagt
     4021 ggcctttggc ttcgacgaga tcgagacaac catgcgggtg tcgcgcaatg cctggtccaa
     4081 tgcgctggcc tgcgcggtgg gtggcgccgt cgggcggtgg ggcaccttgt tccagtgttc
     4141 cagcgaggag gccgaggaac tgcgaatcgg aatggccggc tttacctcct acgcggaaac
     4201 cgtgtccgtc aacggcaccg agacgacctt catcgacggc gacgacacgc cctggtccaa
     4261 ggccttcctt gccgcggcct acgcctcgcg cggcatcaag aagcgctgca cctccggtgc
     4321 aggctccgaa ctgctgatgg gatttcacga gtccaagtcg ctgctctatc tcgaggcgcg
     4381 ctgcctgtgc ctgcagcgcg gcatgggcgt gcagggcacc cagaacggcg gcatcgacgg
     4441 tgcgccgctg accgccacga tgccgggcgg catacgcgaa ctcatggcgg aaaacctcat
     4501 cgctgtctgg ctcgacctcg aatgcgcgtc gggcaacgat gcgcgctcga ccgagtcgga
     4561 gatccgcgtc ggggccaaga tcctgcccta tctcgtcgcc ggatcggacc tgatctgctc
     4621 cggtttcggt tcgatcctga aatacgataa ttccttcaat ccctctctgc tcaatggcga
     4681 agagctggag gactatctgg tgctgcagcg cgacttcgag gccgatggcg ggctgacgcc
     4741 gctgccggag gaacgggcac tggacctgcg caggcgggcc attgcggccg tgtcagcggt
     4801 cttcgaggag ctggagctgg cgcagccgac gaccgagatg aagcagagcg tggccgtggc
     4861 ttcgggatcg gacgagaccc gcagtttcaa tccgcgggac gtgagcttca tcagcgaagc
     4921 gataaaggac cgcggcatcg gtgtgatcga tgtgatcaag gcgctggccc ggcgcggttt
     4981 ccgcgaagag gcagagaacc tgctcaatgt cgtgaagctc cgggtttcgg gcgactacct
     5041 gcagacctcg gcgatgatcc gcgacggcaa ggtcatcagt gcggtgaatg atcccaacac
     5101 ctatctcggt cccggcaccg gttatcgcgt gaccgaggag cggcgccagc agttgaatgc
     5161 catccgcgac gtgctcgacc agaaggaggt attgcgctcg gaggcgatgc acgagaagga
     5221 agaggcccgc cgcatccggt atcgcattct gggcgcggcc ctgcccggca cggcgcccgg
     5281 cgatatcgtg atcggcatca gcccggcctt cggcctgaag ctgttccaga ccacagcagg
     5341 ccaccgactg tcggacgtcc tgcacgagat catgtcggcg atccgcgcgc ggggactgac
     5401 gccacgggtc gtacgcttcc tgcacactgc cgatacctcc tttctcgggc tctccgccgc
     5461 ccggctggcc ggcagcggca tcggtatcgg gctgcaggcc aaggggacgg ccgtcatcca
     5521 cagcaaggat cggctgccgc acaacaatct cgaactcttc tcgaacgcac cgatcacgca
     5581 gctcgcccac taccgtggcc tcggtgccaa tgccgcggcc tatgcgctgg gtgaaatgcc
     5641 cgagcctgtc gtcgtgccgc agcggggcga ggccatgggt tcgcgctatc atgctcgggt
     5701 ggcgctgatc tatgcgatcg agacggggct caccgccgag ggtgccgccc cggaagagat
     5761 cgaggtcaca ttgacgggag cgaagccatg acattgagcc ggaccgacta tcccctggcc
     5821 gagactcagc ccgacaggat ccgggggaag cgtggcctcc gcctgaccga catcaccctc
     5881 gatgccgtga tggcgggcga tatcacaatg gaggatctcc gcatcacgcc cgatgcgctg
     5941 agggcccagg cggacatcgc gcgcgatgcc ggccggccga cgctggcgct caacttcgaa
     6001 cgcgcggccg aactcgtcga tgtgccccag gactttatca tgcaggtcta tgagctgctg
     6061 cgacccggcc gtgcacggtc aaggcagcag ctcctggacg ccgcggcgac ccttcgccgg
     6121 gaccatggcg ccgagcgcat ggctggcttc gtggaggagg ccgcgcaggc ctacgaagac
     6181 cgcggactgt tcacctaccg cttctgagga ttgccgatga gctggaagac caagtaccgt
     6241 tcgctttatt acatgggcgc gccggagccg gatgatcccg tgctcgatcc caaggagacc
     6301 gcgctgctcg tcatcgatgt ccagaatacc tacctggagc ggccggacca gtcttcactc
     6361 tcgccggaag agcagcgccg ctacgatctc tggacgccgt tccatgaccg catgcggacg
     6421 tcggtcattc cgaacacggc aaggatgctg
//
LOCUS       DBNGVR010000498         2526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3132907, whole genome shotgun sequence.
ACCESSION   DBNGVR010000498 DBNGVR010000000
VERSION     DBNGVR010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3132907"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="AC16A0_10405"
     CDS             <1..1764
                     /locus_tag="AC16A0_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3105791.1"
                     /translation="GSGDSEGILEGESLKQEQDDAVTILAWLAAQPWCTGKVGMIGIS
                     WGGFNGLQVAARRPPELQAVISICSTDDRYADDIHYMGGALLTDKISWGSTMFSLNAA
                     PPDPASCGAAWRDLWKQRLKHSGFWLEDWLRHQRRDAFYKHGSVGENWDAITCPVYAV
                     GGWADGYTNAIGRLLDNLKGPRKGLIGPWAHKYPHFAKPGPQIGFLQECLRWWDCWLK
                     GIDTGIMDEPMLRAWIEDPIRPSPYNAEKPGRWVAEKAWPGAGQPLELSLSPGMLGAP
                     GQGTVTISSPQTTGQGAGKWCAYGVWADQPLDQRVEMGGQAVFDTEPLAEDLDILGTP
                     LLSITLSCDQPQALLAATLCEVFPDGAATRISYGILNLSHRESHENPSPLQPGKACRV
                     TLKLCDAGHRFGKGNRIRLSLSNAYWPIVWPSPKSNMLNIDCAPSALRLPLRASRPED
                     DLLAEFPPAETAPPLRQTQLDLGHNRWTVTSDMMANTSVLERSNDDGWHRIEDTGLEL
                     ALHTTQRYTIQPDDPTSALLETRHVRRYRREDWAVTIVTDIALRSDAAQFHVVAKLDA
                     REDDAIVSQQTWSFSVPRDHL"
     gene            complement(1771..>2526)
                     /locus_tag="AC16A0_10410"
     CDS             complement(1771..>2526)
                     /locus_tag="AC16A0_10410"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3105792.1"
                     /translation="QAERSQVPIIAVPTTAGTGAEVEASGIITNTAKPVKAGIVNLSM
                     MPRVVIADPELTLSLPPNLTAWTGMDALSHNLEALLVRQFHPMADAIGAVGVRMCHDW
                     LPRAVENGSDLTARSYMMAAAIMGATSFVKGLGAMHALSHAIGALVNTQHGLTNAVLM
                     PYVMRFNRPAIEEPVAALAAYMGLPPHFDAYMKAMLDLSGRIGIPGKITDIGVDRSDF
                     RRLAIMALPDFNAGCNPRPLDVDGLVSILEAAC"
BASE COUNT          458 a          805 c          811 g          452 t
ORIGIN      
        1 ggctccggcg actccgaggg cattctcgaa ggcgagtctc tgaaacagga acaggacgat
       61 gccgtgacga tccttgcctg gcttgcggcg cagccgtggt gcaccggcaa ggttggcatg
      121 atcggcattt cctggggcgg gttcaatggt ctgcaggttg ccgctcggcg tcctcccgag
      181 ctgcaggcgg tgatttcgat ttgctcgacc gacgaccgct atgccgacga cattcactac
      241 atgggcggcg ccttgctgac cgacaagatc agctggggtt cgacgatgtt ctcgctcaat
      301 gccgcgcctc ccgacccggc aagctgcggt gcggcctggc gcgacttgtg gaagcagcgg
      361 ctgaaacaca gcggattctg gctggaagac tggttgcgcc atcagcgccg cgacgccttc
      421 tacaagcatg gttcggtcgg cgagaactgg gacgcgatca cctgtcccgt ctatgccgta
      481 ggcggctggg ccgatggcta tacaaatgcc atcggccgcc tgctcgacaa tctgaaggga
      541 ccccgcaagg gcctgatcgg gccctgggcc cacaagtatc ctcactttgc caagccaggc
      601 ccgcagattg gctttctcca ggaatgcctg cgctggtggg actgctggct gaagggtatc
      661 gatacgggaa tcatggacga gcccatgttg cgagcctgga tcgaggaccc gatccggccg
      721 tcgccctaca atgccgagaa gcccgggcgc tgggtggccg agaaagcctg gccgggcgca
      781 ggccagcccc tcgaattgtc tttgtcgccc gggatgctgg gtgcgccagg ccagggaaca
      841 gtgacaatct cctcgccgca gaccacggga caaggggcgg gcaaatggtg cgcttatggc
      901 gtctgggccg atcagccgct ggaccagcgg gtggaaatgg gcgggcaggc cgtcttcgac
      961 acggaaccgc tcgccgaaga cctcgacatc ctcgggacgc cgcttctttc gatcacgctg
     1021 tcctgcgatc agccgcaggc gcttcttgcc gccaccttgt gcgaggtctt tcccgacggc
     1081 gcagcgacgc gcatcagcta tggcatcctc aatctctcgc atcgcgagag ccacgaaaac
     1141 ccgtcgcccc tccaaccggg gaaggcttgt cgcgtcacgc tgaagctctg cgacgcgggg
     1201 catcgcttcg gcaagggcaa caggatccgg ctgtcgctgt ccaatgccta ttggccgatc
     1261 gtctggcctt cgccgaagtc aaacatgctg aacattgatt gcgcgcccag tgccttgcgc
     1321 ctgcccttgc gggcttcgcg gcccgaagac gacttgcttg cggagttccc gccagccgag
     1381 actgcgcccc cgctgcgcca gacccagctc gatcttggac acaatcgatg gaccgtcaca
     1441 tcggacatga tggccaatac ttctgtgctg gagcgcagca acgacgacgg gtggcaccgg
     1501 atcgaggaca ccgggctcga gcttgccctg cacacgacgc agcgttacac catccagcct
     1561 gatgacccga catcggcgct gctcgaaacc cgtcacgtgc gccgctaccg cagggaggat
     1621 tgggccgtca cgatcgtgac cgacatcgcg ttgcgttcag acgccgcgca attccatgtg
     1681 gtcgcgaagc tcgatgcgcg cgaagacgat gcgattgtct cgcaacagac ctggtccttc
     1741 tcggttccgc gcgatcatct ctagcaaggg tcagcacgcg gcctcgagaa tggagaccag
     1801 tccatcgacg tccagcgggc gcggattgca gcccgcgttg aagtcaggca gcgccatgat
     1861 ggcgaggcgg cggaaatcgc tgcgatccac gccgatgtcg gtgatcttcc cggggatgcc
     1921 gatccgaccc gaaagatcga gcatggcctt catgtaggca tcgaagtgcg gcggcagccc
     1981 catgtatgcc gcgagggccg cgaccggctc ctcgatcgca ggccggttga accgcatcac
     2041 atagggcatc agcacggcat tggtgagccc gtgctgcgtg ttgaccagag cgccaatggc
     2101 gtggctcagc gcatgcatgg cacccaggcc cttgacgaag gaggtcgccc ccatgatggc
     2161 tgcggccatc atgtaggaac gtgccgtgag atcactaccg ttctcgacgg cccgcggcag
     2221 ccagtcatgg cacatcctga cgcctaccgc gccgatggca tcagccatgg gatgaaactg
     2281 ccggaccagc agggcctcga gattgtgcga gagcgcatcc atgccggtcc atgctgtgag
     2341 gttcggtggc agcgacaggg taagttcggg atcggcaatc acaacgcgcg gcatcatcga
     2401 gagattgacg atgccggcct tcaccggctt ggcggtgttc gtgatgatgc cgctggcctc
     2461 gacttccgct ccggtacctg ccgtcgtcgg aacagcgatg atcggcacct ggctgcgctc
     2521 ggcctg
//
LOCUS       DBNGVR010000499         3224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3919858, whole genome shotgun sequence.
ACCESSION   DBNGVR010000499 DBNGVR010000000
VERSION     DBNGVR010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3919858"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="AC16A0_10415"
     CDS             <1..450
                     /locus_tag="AC16A0_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3105793.1"
                     /translation="VTNCEVISAQDQGKLEAAAPFDCVVIDAPCSGSGAWRRKPDAKW
                     RLTETQLDKRLKDQRQVLEKGFGLVRPGGRLVYITCSVLAQENTAQVADFLQRHPGCR
                     LIPYTDQWRSAIGGEPPFSADGSQDTLLMTPARHGTDGFFVAVIGKG"
     gene            complement(456..896)
                     /gene="mntR"
                     /locus_tag="AC16A0_10420"
     CDS             complement(456..896)
                     /gene="mntR"
                     /locus_tag="AC16A0_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HIR3105794.1"
                     /translation="MPRPAKHQRGAKLRSAAFSRIRSDHQSELAEDYVELIADLIDER
                     GEARGTDVAMRLGVANATVVKTLKRLQDAGLVTQEPYRAIFLTGEGWKMAEDGRRKHR
                     IVEGFLLALGVSEETARIDSEGIEHHVSEETLRAMARFLARGGR"
     gene            967..2313
                     /locus_tag="AC16A0_10425"
     CDS             967..2313
                     /locus_tag="AC16A0_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR3105795.1"
                     /translation="MPQDTTIADRLSGWRRPPGEPPLADVFRTVRVASSGSTWRRAMA
                     FIGPGYLVAVGYMDPGNWATSIAGGAQFGYALLAVALMSNIMAIILQSLCARLAVGSG
                     RDLAQACRDAFPRPVAFVLWIFAEIAIIATDIAEVVGTAIGLNLLFGIPLELGVIITA
                     LDVFLILWLQRIGFRYIEAFIIALLGVIFVCFFIQIALADPEWGQVIRGFAPTVEIVS
                     NPAMLYLALGIIGATVMPHNLYLHSGIVQTRAYGDSLPEKREALKFATIDSTVALMFA
                     LLVNASILILAAATFHRGGHTSVTDIGEAHSMLAPLVGSLLAPKLFAIALLCCGLNST
                     VTATMAGQIVMEGFLDIKLAAWARRLITRLIAIVPAAGVTLYYGAEGTGLLLILSQVI
                     LAFQLPFAIVPLVMFTRDRAKLGALSAPVWLTLVAGTIAAIIIALNVKLLWDIATG"
     gene            2360..>3224
                     /gene="guaA"
                     /locus_tag="AC16A0_10430"
     CDS             2360..>3224
                     /gene="guaA"
                     /locus_tag="AC16A0_10430"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3105796.1"
                     /translation="MQDRILIIDFGSQFTQLIARRIREAGVYSEIVPFQLAEKALDWE
                     GLKGIILSGGPASVHEGGSPRAPERVFTRGVPVLGICYGEQTLCAQLGGKVEPSDHRE
                     FGKAFVEVKSANALFDGVWSAGERHQVWMSHGDRVTAIPPGFKAIAVSEGAPFAAIAD
                     ESRQFYATQFHMEVVHTPDGARLLRNFALEICGCRGDWSMAHFKTEMVHRIKKQVGFD
                     RVICGLSGGVDSAVTAVLIHEAIGEQLTCIFVDHGLMRLNEADEVVRLFRNTYNIPLI
                     HVDASAQFLGAL"
BASE COUNT          483 a         1117 c         1011 g          613 t
ORIGIN      
        1 gtcacaaact gcgaggtcat ctcggcgcag gaccagggca agctggaggc ggccgcaccc
       61 ttcgactgcg tggtgatcga tgcgccctgt tcgggttcag gtgcctggcg gcgcaagccc
      121 gacgccaagt ggcgtctcac cgaaacgcag ctcgacaagc gtctcaagga tcagcgccaa
      181 gtccttgaaa agggattcgg attggtccgc cccggcgggc ggctggtcta cattacatgc
      241 tccgtgctgg cgcaggagaa caccgcccag gtggcagatt tcctgcagcg ccatcccggc
      301 tgcaggctca ttccgtacac ggatcaatgg cgcagcgcga tcggcggcga accgcctttc
      361 tctgccgatg gttcgcagga caccctgctg atgacccccg cccggcatgg caccgacggg
      421 ttcttcgtgg cggtgatcgg aaagggctga acggcctacc tgccgcctcg cgccaggaag
      481 cgcgccatgg cccgcagcgt ctcctcgctc acgtggtgct cgatgccttc ggaatcgatc
      541 cgcgccgttt cctcgctcac gcccagcgcc agcaggaaac cctccacgat gcggtgcttg
      601 cggcggccgt cctcggccat tttccagccc tcgccggtga ggaagatggc gcgatagggc
      661 tcctgggtga cgaggccggc atcctgcagg cgcttgaggg tcttcaccac ggtggcattt
      721 gccacgccaa ggcgcatggc cacgtcggtg ccgcgcgcct cgccgcgctc gtcgatcaga
      781 tcggcgatca gctcgacata gtcttccgca agttccgact ggtggtcgga ccggatccgg
      841 ctgaaggcgg cggaccgcaa cttggccccg cgctggtgtt ttgccggtcg cggcatcctg
      901 gctctccgtg tgcggttgcg aaattaccct attgcaatag atttagccaa tgctaaactt
      961 tttgtaatgc cgcaggacac caccatcgcc gatcgcctct ccgggtggcg ccgcccaccc
     1021 ggtgagccgc cgcttgccga tgtcttccgt accgtgcggg tggcttcgtc cggatccaca
     1081 tggcgccgtg ccatggcctt catcggcccc ggctatctgg tcgcggtcgg ctacatggac
     1141 cccggcaact gggccacctc catcgccggc ggggcacagt tcggctacgc gctgctggcc
     1201 gtggcgctca tgtccaacat catggccatc atcctgcagt cgctctgcgc gcggctcgcg
     1261 gtgggatcgg gccgcgacct ggcgcaggcc tgccgcgacg cctttccgcg tccggtggcc
     1321 ttcgtgctgt ggatcttcgc cgagatcgcc atcatcgcaa ccgacatcgc cgaggtggtg
     1381 ggcacggcca tcggcctgaa ccttctcttc ggcatcccgc tggagctggg ggtgatcatc
     1441 acggcgctcg acgtcttcct tatcctgtgg ctgcagcgca tcggcttccg ctacatcgag
     1501 gccttcatca ttgcgctgct gggcgtcatc ttcgtctgct tcttcatcca gatcgcgctc
     1561 gccgatccgg agtggggcca ggtcatccgc ggcttcgcgc ccacggtgga gatcgtcagc
     1621 aacccggcca tgctctatct ggcgctcggc atcatcggcg ccacggtcat gccgcacaat
     1681 ctctatctcc attcgggcat cgtccagacg cgggcctatg gcgacagcct gccggaaaag
     1741 cgcgaggcgc tgaagttcgc caccatcgat tcgaccgtgg cactgatgtt cgcgctcctg
     1801 gtcaacgcgt cgatcctcat cctggcggcg gcaaccttcc accgcggcgg ccacacctcg
     1861 gtcaccgaca tcggcgaggc gcattccatg ctggcgccgc tggtgggatc gctgctggcg
     1921 cccaagctct tcgccatcgc gctgctgtgc tgcggtctga actcgacggt caccgccacc
     1981 atggccgggc agatcgtcat ggagggcttc ctcgacatca agctggcggc ctgggcgcgg
     2041 cggctcatca cccggctcat cgccatcgtc ccggccgccg gcgtcacgct ctactacggc
     2101 gccgagggca ccggcctgct gctgatcctc agccaggtca tcctcgcctt ccagctgccc
     2161 ttcgccatcg tgccgctggt catgttcacc cgcgacaggg ccaagctcgg cgccctttcc
     2221 gcgccggtct ggctcacgct ggtggcgggc acaatcgcgg cgatcatcat cgcgctcaac
     2281 gtgaagctgt tgtgggacat cgcgacgggc tgattcctcc gcacttgcag actccggtcg
     2341 cttcccttat gcgctcgcca tgcaagaccg aatcctgatc atcgacttcg gctcccagtt
     2401 cacccagctg attgcccggc gcatccgcga ggcaggggtc tactcggaaa tcgtcccttt
     2461 ccagctggcg gaaaaggctc tcgactggga gggcctgaag ggcatcatcc tgtcgggtgg
     2521 cccggcctcc gtccatgagg ggggaagccc ccgggccccg gaacgggtct tcacccgggg
     2581 agtgccggtt ctcggcatct gttacggcga gcagaccctc tgcgcccagc tgggcggcaa
     2641 ggttgagcca tccgaccatc gcgagttcgg caaggccttc gtcgaggtga aatcggccaa
     2701 tgcgctcttc gacggcgtgt ggagtgccgg cgagcgtcac caggtctgga tgagccacgg
     2761 cgaccgcgtc accgccattc cgcccggctt caaggccatc gcggtgagcg agggcgcgcc
     2821 cttcgccgcg atcgccgacg agtcccgcca gttctatgcc acgcagttcc acatggaagt
     2881 ggtgcacacg cccgatggcg cgcggctttt gcgcaacttc gcgctggaga tctgcggctg
     2941 ccgcggcgac tggtccatgg cccacttcaa gaccgagatg gtccatcgca tcaagaagca
     3001 ggtgggtttc gaccgggtga tctgcgggct gtcgggcggc gtcgattccg ccgtcaccgc
     3061 ggtgctgatc cacgaggcca tcggcgagca gctcacctgc atcttcgtcg accatggcct
     3121 gatgcggctc aacgaggccg acgaggtcgt gcggctgttc cggaacacct acaacatccc
     3181 gctcattcac gtggatgcga gcgcgcagtt ccttggcgcg ctcg
//
LOCUS       DBNGVR010000500         1874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4310805, whole genome shotgun sequence.
ACCESSION   DBNGVR010000500 DBNGVR010000000
VERSION     DBNGVR010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4310805"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..631)
                     /locus_tag="AC16A0_10435"
     CDS             complement(179..631)
                     /locus_tag="AC16A0_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105797.1"
                     /translation="MRSKQTLHNSLSLPALLLAAGFAAAPAMAAQFDFMAAPQTDLNR
                     IYRIDRVTGEVGACQYGQKEGTFGVTLCFPAGDGAGKQEPSEYGLVSSRHDREGGVFR
                     VDMRTGAMSVCYVFDERVVCTPPEKGAVSDAGAKAGTTGDAGSSTRKP"
     gene            880..1587
                     /locus_tag="AC16A0_10440"
     CDS             880..1587
                     /locus_tag="AC16A0_10440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3105798.1"
                     /translation="MSAANQPTDLPTLVFDLDGTLADTAIDLIGTLNVLMKRMGAPEV
                     PVDRARDVVGAGARAMIQRGLALGGRTVSDSEIEALFRDFLVHYAENIAVKTELYPGV
                     RAALDRLALAGYRLAVCTNKPEEHSIRLLAALGVADLFGSICGRDTFAYAKPDPRHLT
                     LTVEKAGGDPGHAIMIGDSRTDVDTARAAGLPVIAVSFGYTDTPVEKLGPDRIVDHFD
                     ELESAILAFRRPMADAG"
     gene            complement(1594..>1874)
                     /locus_tag="AC16A0_10445"
     CDS             complement(1594..>1874)
                     /locus_tag="AC16A0_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR3105799.1"
                     /translation="RVTELHEMAMTNGVMTMRPRPAGIEIPAGGKVELKPGGLHVMLI
                     GLAKPLVKGEKVKARLVFEKAGSIDVEFEVEGIGAGSAGSHNSGAHAH"
BASE COUNT          288 a          648 c          617 g          321 t
ORIGIN      
        1 cgccacgatg atgcgcgtat gtctcacgaa agccccatgg cctggcttgc ttggcctggc
       61 ttgcttcgcc tggcttgcac ggcctggctg gaaagtgttt ggccgagacg cattggactc
      121 gaccggcggc tctctagcat gccttggctg cgaaggtgaa gcgcgtccgg ggccgggcct
      181 acggcttgcg ggtgctcgaa ccggcgtcgc ccgtggtgcc agccttggcc ccggcatccg
      241 ataccgcgcc cttttcgggc ggggtgcaga ccacccgctc gtcgaacaca tagcagacgc
      301 tcatcgcccc cgtgcgcatg tcgacgcgga agacgccgcc ctcgcgatcg tgccgggagg
      361 agacgaggcc atattcggag ggctcctgct tgccggctcc gtcaccggcg gggaagcaca
      421 gggtcacgcc gaatgtgccc tccttctgcc cgtactggca cgcgccgact tcgccggtga
      481 cgcgatcgat gcggtagatc cggttcaggt cggtctgcgg cgcggccatg aagtcgaatt
      541 gcgcggccat ggcgggcgcg gcggcaaacc ctgccgccag cagcagggcc ggcaggctga
      601 gcgagttatg gagcgtctgc ttcgatctca tggaacgtct cgggctgttc ggtgcgggca
      661 tcccatggct cggcgctggc ggctcagccg attccggaag ggtccgcttc actgtgtccc
      721 cgcatggtta acagtttatg accggccgca acgctacgac gcatcgcagg cccggcaccc
      781 gcgccgcaag gcattcgcgt gcgcaactgc ccgggatcgc cgcccggccg gagccctgca
      841 atcgttgacg tcgccgcaag gctccggcaa acacaagaca tgtcagccgc gaatcagccc
      901 actgatctgc cgacgctcgt cttcgatctg gacgggacgc tcgccgacac cgccatcgac
      961 ctcatcggca cgctcaacgt gctcatgaag cggatgggag caccggaggt gcccgtcgac
     1021 cgggccaggg acgtcgtggg cgcaggcgcg cgggcgatga tccagcgcgg tctcgccctt
     1081 ggcggcagaa cggtctcgga ttcggaaatc gaggcgctgt tccgggactt tctggtccac
     1141 tacgccgaga acatcgctgt caagaccgag ctctatcccg gcgtacgggc ggcgctcgac
     1201 cggctcgccc tggctggcta tcggctggcg gtctgcacca acaagcccga ggagcactcg
     1261 atccgcctgc ttgccgcgct tggcgtggcg gacctcttcg gctcgatctg cgggcgcgac
     1321 acgttcgcct atgccaagcc ggacccgcgc cacctcacgc tgacggtgga gaaggccggc
     1381 ggagaccccg gccacgcaat catgatcggc gattcccgga cggacgtgga caccgcgcgc
     1441 gccgcggggc ttcccgtcat cgccgtgagc ttcggctaca ccgacacgcc ggtggaaaag
     1501 cttgggccag accgcatcgt ggatcatttc gacgagctgg aatcggcgat tctcgccttc
     1561 cggcggccga tggcggacgc cggctgaagc cgatcagtgc gcgtgagcgc cggaattgtg
     1621 gctgccggcg gatcctgcgc cgatgccctc gacttcgaac tccacgtcga tggatcccgc
     1681 cttctcgaac accagcctgg ccttgacctt ctcgcccttg acgagcggct tcgccagccc
     1741 gatcagcatc acgtgaaggc cgccgggctt caattccacc ttgccgccgg ccggaatctc
     1801 tatgcccgcc gggcgcggcc gcatggtcat gacgccgttc gtcatggcca tctcgtgcaa
     1861 ctcggtgacg cgcg
//
LOCUS       DBNGVR010000501         4093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2741507, whole genome shotgun sequence.
ACCESSION   DBNGVR010000501 DBNGVR010000000
VERSION     DBNGVR010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2741507"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="AC16A0_10450"
     CDS             <1..1454
                     /locus_tag="AC16A0_10450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR3105800.1"
                     /translation="DAHAANAQTGEKQGCIMSFERPRGIAVADVGYTNTKIMLFSPDL
                     VPVAERKIASRHRVGPPYRNIDPEPMAALCREALPELDRILPVDAVVPCAHGAALACL
                     AADGSLAMPVMDYTSEPPPDIVADYRRIAPGFEETFCTLLPMALTHGLQLYWQQRAWP
                     ADFARIATVIPWIQYVGFRLSGKAVTEMSSMSCQTHLMDTRSQTLSSLVRRQGWEALF
                     PPMAKAWETIGRLRPEFTGPGFRGRGAVLGGVHDSTANYMRYVCAGLDRFSLVSTGTW
                     SISFDTSTPLDHLVEARDTNTNTDVLGRTVACSRFFGGKEFEQVAGDAPAEAASLDMV
                     RQLIAGGVMALPSFTDSGGPVPRSGGRGRIVGDLPASAEARASLAALYCAQMVSEQLD
                     AISSRHEVIVDGPFAQNPVLMAILADLRRGQQVKASDLRDGTTVGAASLALIDDGRLP
                     AIGLSLKLQAPPGLAGLARYHSNWKEKAYAHLA"
     gene            1438..2415
                     /locus_tag="AC16A0_10455"
     CDS             1438..2415
                     /locus_tag="AC16A0_10455"
                     /inference="COORDINATES: protein motif:HMM:NF018684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR3105801.1"
                     /translation="MRIWLSLAAVLLAANPAQAFDCAKAATPVEKAICGDDTLKSLDD
                     RMSEAYGAVRKLSTETERPMLTRSQKAWIAEREANCRKSEIGMVPCVSDMTRERLKLF
                     EGKPESGTAPDSRVIPVFILQEGTPEVYELDVTMLRFADARTPGEKRFNAIADEIGAR
                     VKTGKHGEDTMDRVYSMIEAMSFRFAAPRLYSVEHSFWSFTGGAHGNGGTQNFNFDPV
                     TGKDYTVDDVFPEDAVVKLIASCKDKIIAEKKIRWGSEPYDPAQDFSMQDGVISEHVA
                     TFSSWSFYAGGACLNFDSYAIGSYAEGTYACDFPMAELKTLARPDSPLP"
     gene            2470..2880
                     /locus_tag="AC16A0_10460"
     CDS             2470..2880
                     /locus_tag="AC16A0_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105802.1"
                     /translation="MRLAMHADQLPNPAGPVFSLNCPGYRMARGGGTLPFSIGDAVRA
                     EISHDDLLESWQEARGLTPTEAEKMTAAAAAQLTRILESGLESADITEAVTDAAVVLL
                     LALRRQGISNPKRIPPCTVIWHGQDNREHVLLGA"
     gene            2877..3320
                     /locus_tag="AC16A0_10465"
     CDS             2877..3320
                     /locus_tag="AC16A0_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3105803.1"
                     /translation="MTDSSTDREPPVSAPDYGRSLRGIGFNLLVTDVPRAVRFVTEVL
                     GATSTYDNGSFAALRLNGGEFMFHADHTYRGNPLLGVVAGVEGRGAGVELRIYGCDPD
                     LAEARARALGYTVLAGSIDKPHGLRECMIMDDDGYVWVPGAAIRG"
     gene            complement(3375..3770)
                     /locus_tag="AC16A0_10470"
     CDS             complement(3375..3770)
                     /locus_tag="AC16A0_10470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR3105804.1"
                     /translation="MAGLHFELVSPARLLFSGSVASVTIPGTEGEMGIFPGHAPILST
                     LKPGVVTVYKDGGGTDRIFVRGGMVEVNPQGLTLLAEVAIPMSELDAAQLGQQIKHAE
                     EDLADAKDGEDKRKAAETLEHLKALRAAI"
     gene            complement(3776..>4093)
                     /locus_tag="AC16A0_10475"
     CDS             complement(3776..>4093)
                     /locus_tag="AC16A0_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase beta subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR3105805.1"
                     /translation="VQAILQRYKALQDIIAILGMDELSEEDKLTVARARKIERFMSQP
                     FDVAAVFTGSPGVQVKLEDTIKGFKGLCDGAYDHLPEPAFYMVGTIEEAIAKAQRLAA
                     EAA"
BASE COUNT          703 a         1380 c         1316 g          694 t
ORIGIN      
        1 ttgacgccca tgcggcgaat gcgcagactg gcgaaaaaca gggttgcatc atgagtttcg
       61 aacgcccgcg gggcatcgcc gtcgccgacg tcggatacac caacaccaag atcatgctct
      121 tttcgcccga cctcgtgccc gtggcggagc gcaagattgc cagccggcac cgcgtgggac
      181 ctccctatcg caacatcgac cccgaaccca tggcggcgct gtgccgcgag gccttgcccg
      241 aactcgaccg gatcctgccg gttgacgctg tcgtcccctg cgcccatggc gcggcgctgg
      301 cctgcctggc cgccgacggc agtctcgcca tgccggtcat ggactatacc tccgagccgc
      361 cgccggacat cgtcgcggac taccgccgga tcgcaccggg cttcgaggag accttctgca
      421 ccctgctgcc gatggctctg acccacggcc tgcagctcta ctggcaacag cgggcctggc
      481 cggcagactt cgcccgcatc gccacggtga tcccctggat ccagtatgtc ggcttccggc
      541 tcagcggcaa ggcggtgacc gaaatgtcga gcatgtcgtg ccagacccat ctgatggaca
      601 cgcgcagcca gacactctcg tcgctggtaa ggcggcaggg ctgggaagcc cttttccccc
      661 ccatggccaa ggcctgggag accatcggcc ggctcaggcc cgagttcacc gggcctggct
      721 tccgcggccg cggcgccgtg ctgggcggcg tgcatgactc gaccgccaac tacatgcgct
      781 atgtctgtgc cgggctggac cgcttttcgc tcgtctcgac cggaacctgg agcatcagct
      841 tcgacacctc gacgccactc gatcatctgg tcgaagcgcg cgacaccaac accaataccg
      901 atgtgctggg ccgcacggtg gcctgcagcc ggttcttcgg cggcaaggaa ttcgagcagg
      961 tggcgggcga cgcgcctgcc gaggcggcct ccctcgacat ggtgcgccag ctgatcgccg
     1021 gcggtgtcat ggccctgccc tccttcaccg attccggcgg cccggttccg cgctcgggcg
     1081 ggcgcggacg gatcgtcggc gaccttccgg cgagcgccga agcccgcgcc tcgctcgcgg
     1141 ccctctattg cgcccagatg gtgtcggagc agctcgatgc catttcgtcc cgtcacgaag
     1201 tcatcgtcga cggacctttc gcgcagaatc ccgtgctgat ggccatcctc gccgacctcc
     1261 gccgcgggca gcaggtgaag gcctcggact tgcgcgatgg aacgacggtg ggcgcggcga
     1321 gcctcgcact catcgacgac ggcaggcttc cagccatcgg cctgtcgctc aagcttcagg
     1381 cgccgccggg tcttgcggga cttgcccggt accattcgaa ctggaaggag aaggcctatg
     1441 cgcatctggc ttagtcttgc cgcggtgttg ctggccgcca acccggccca ggccttcgac
     1501 tgcgccaagg ccgcgacgcc ggtggagaaa gctatctgcg gcgatgacac gctcaagtcc
     1561 ctcgacgacc gcatgagcga ggcctatggc gccgtgcgca agctctccac cgagaccgag
     1621 cgccccatgc tgacacggtc gcagaaggcc tggatcgccg agcgcgaagc caattgccgg
     1681 aagtccgaga tcggcatggt tccctgcgtc agcgatatga cccgcgagcg gctgaagctg
     1741 ttcgagggca agcccgagag cggcacggca cccgacagcc gtgtcatccc tgtgttcatc
     1801 ctgcaggagg gaacccccga ggtctatgaa ctcgacgtca ccatgctgcg ttttgccgat
     1861 gcgcgcacgc cgggcgagaa acggttcaat gcgatcgccg acgagatcgg ggcgcgcgtc
     1921 aagaccggca aacacggcga ggacaccatg gaccgcgtct actccatgat cgaggcgatg
     1981 tccttccgct ttgccgcacc gcgtctgtac tcggtcgaac acagcttctg gtctttcacc
     2041 ggcggcgcgc atggcaatgg cggcacccag aacttcaact tcgatccggt gaccggcaag
     2101 gactacacgg tggatgatgt cttccccgaa gacgcggtcg tgaagctgat cgccagctgc
     2161 aaggacaaga tcattgccga gaagaagatc cgctggggca gcgaaccata cgatccggcg
     2221 caggacttct ccatgcagga cggcgtgatc agcgaacatg tggcgacctt cagcagctgg
     2281 agcttctatg ccgggggggc gtgcttgaac ttcgactcct atgccatcgg ctcctatgcc
     2341 gaggggacct acgcctgcga ttttccgatg gccgaactca agacgctggc acggcccgat
     2401 tcgcccctgc cgtgagccgg aacatcgtta accgatcgga aacacaagag gcgcagcatg
     2461 gagcaaacca tgaggcttgc catgcatgca gatcagttgc ccaacccggc cgggcctgtc
     2521 ttttcgctga actgccccgg ttacaggatg gcccgcggcg gagggacatt gcccttcagc
     2581 atcggcgacg cggtgcgcgc cgagatcagc catgacgacc ttctggaaag ctggcaggaa
     2641 gcccgcggcc tgacgccgac cgaggccgag aagatgacgg ccgccgccgc cgcgcaactg
     2701 acccgcattc tcgagagcgg cctggagtcg gccgacatca cggaagccgt gaccgacgct
     2761 gccgtggtgc tgctcctggc cctgagacgg cagggcattt cgaatccgaa gcggattcct
     2821 ccatgcaccg tgatctggca tggtcaggac aaccgggagc atgttctgct gggggcatga
     2881 cggactcctc cacggacagg gaaccgccgg tctccgcgcc cgactacgga cggtcgctgc
     2941 gcggcatcgg cttcaatctg ctggtcaccg acgtgccgcg cgcggtgcgc ttcgtgacgg
     3001 aggtgctggg cgccaccagc acctatgaca atggcagttt cgccgcactc cggctcaacg
     3061 gcggcgagtt catgttccat gctgatcaca cttaccgcgg caatccgctg cttggcgtgg
     3121 tggcgggcgt cgaaggccgg ggcgcgggcg tcgaactgcg catctatggc tgcgaccccg
     3181 acctggccga agcgcgggcg cgcgcgttgg gctataccgt gctcgctggc agcatcgaca
     3241 agccgcatgg cctgcgcgaa tgcatgatca tggatgacga cggctacgtc tgggttcccg
     3301 gggccgcgat ccgcggataa gaaaagcccg cgggctggcc gcgggctctc gaatcctgga
     3361 aaagaccaac cgcgtcagat cgcggcgcgc agcgccttga ggtgttccag cgtttccgcc
     3421 gccttgcgct tgtcctcgcc gtccttcgcg tcggcgagat cttcctcggc atgcttgatc
     3481 tgctgcccca gctgcgcggc atcgagttcc gacatgggga tggccacctc ggcaagcagc
     3541 gtcagaccct gcggattgac ctcgaccatg ccgccgcgca cgaagatgcg atccgtgccg
     3601 ccgccgtcct tgtagacggt gaccacgccc ggcttcagcg tcgagagaat tggcgcatgg
     3661 cccgggaaga tgcccatctc gccctcggtg cccgggatgg tgaccgaggc caccgagccc
     3721 gagaacagaa gccgcgccgg gctcacgagt tcgaaatgaa ggccggccat ctgggttacg
     3781 ccgcctcggc ggccaggcgc tgggccttgg cgatggcttc ctcgatggtg ccgaccatgt
     3841 agaaggccgg ttccggcaga tggtcatagg cgccgtcgca cagccccttg aagcccttga
     3901 tcgtgtcttc gagcttcacc tgcacgccgg gcgaaccggt aaacacggcc gcgacgtcga
     3961 agggctgcga catgaagcgc tcgatcttgc gggcgcgtgc caccgtgagc ttgtcctctt
     4021 ccgagagttc gtccatgccg agaatggcga tgatgtcctg cagcgccttg tagcgctgga
     4081 gaatggcctg cac
//
LOCUS       DBNGVR010000502         4572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3133863, whole genome shotgun sequence.
ACCESSION   DBNGVR010000502 DBNGVR010000000
VERSION     DBNGVR010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3133863"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="AC16A0_10480"
     CDS             complement(<1..771)
                     /locus_tag="AC16A0_10480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3105806.1"
                     /translation="MEGSAVYFDGKTAADILVRLRLTPDALRFHGQDLNEQIWPLSDL
                     EAVDLPHAGRPLRLCRAAMPGARLVLHDEAMGNAILKAAPQLRGGLRLGRMLRPLAWI
                     AGDLVAIAVGGYLVVQLAPEGLARHLPQSWRDRIGSQMEASIVETARPCMGKPGVSAL
                     AAMTARIAESGADLPPLAITVYDLPVMNAFAMPGDRIVITRELISRAASPEEVAGVLA
                     HEIGHAANRHPEAQMVRIMGIQILINLITGGNSDTVTSM"
     gene            complement(771..1904)
                     /locus_tag="AC16A0_10485"
     CDS             complement(771..1904)
                     /locus_tag="AC16A0_10485"
                     /inference="COORDINATES: protein motif:HMM:NF017770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HIR3105807.1"
                     /translation="MTEHAAKGDSIRIHYVPRTGLLRLSLLNALLFLLTLTLYRFWAR
                     TNVRRHIWSCVHINGEPLEYTGKGIELFKGAMFVFLVFGLPTVLAFAGLTLAFGPEHP
                     SVIGLQLLVFLAISLLWGAAIYRARRYQLSRTLWRGIRGALLGSTTTYALLYFGSIMA
                     RAMTLGWITPVMNLTLQQQMMGSTFFGDRPFRFKGPAGPLYPAYALCWFVSLLVIAVL
                     GGLLIAEFTLLESEGFWASLAGEQGGDPGSPDQVSIAIGVIVVSLAVFYLLYLTLYPA
                     IWAMYTARELIQFATYTSFDGAQFRFDATTGSIIRLAIGNALLSVLTLGVAAPFVQQR
                     LVRYLCDRVRIEGTVNIDRIRQSRTPVSSMGEGLADALDVGGL"
     gene            2298..3188
                     /locus_tag="AC16A0_10490"
     CDS             2298..3188
                     /locus_tag="AC16A0_10490"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3105808.1"
                     /translation="MSVGTRPAVLALIALIALVAMSFRAQAAEDADSGPLADFYSYLP
                     ATPELKIPDINIPFWTDDLKKGQRAYRTGKYDRALKSFRKASDEGNTVADWYLGHMYR
                     LGQGVPRNDAMAYSYYSRVADSYDPDEPDQNRLRIAVDAMVRLADYQREGIGEASVKS
                     NPAAAARSYLKIATAYGHPAAQYALGVMNIKGQGVKRNPQQGLKWLMTAARKRYAPAE
                     AYLGELYWEGKIVRRDRTRAVMWYALAKESARPEENPEIYDRALQLEANVSEEELIEA
                     EARARVWDGQYPVDRRRGND"
     gene            complement(3193..3630)
                     /locus_tag="AC16A0_10495"
     CDS             complement(3193..3630)
                     /locus_tag="AC16A0_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HIR3105809.1"
                     /translation="MNRQAIARRKPPQSRGREIVIGTLRKPLLLGTGWLCVGLGALGV
                     VMPLFPTTPFLLVAVWAFSRSSPELAEKIRNHPVVGPFIRDWQDSGVIPTKAKVFAVV
                     MMAAMFAYLHFGAQAPVWIQIGVAVVFLAVSAFIVTRPGRAPQ"
     gene            3648..4412
                     /locus_tag="AC16A0_10500"
     CDS             3648..4412
                     /locus_tag="AC16A0_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR3105810.1"
                     /translation="MTDPSDTPPHLKAVDVEIKTQPSFLGRLRAYFFTGLVVTAPIAI
                     TIWATYWFVTFFDAWVKPFIPSVYNPDTYLSFQVPGFGLLFALITITIIGALAANLVG
                     RTVIGLWDKLLNRTPVVRSIYKGSKQIFETIFAQKGASFRYVCLVEWPRRDCWSIAFV
                     SREVDGGQVGLEPGRDMYAIYVSTTPNPTSGYLFFADVADVKILDMSVEDGLKLVISM
                     GLVFPDRPLPAAEEAKVAAVKTARPKRKPVSRRKSA"
     gene            complement(4390..>4572)
                     /locus_tag="AC16A0_10505"
     CDS             complement(4390..>4572)
                     /locus_tag="AC16A0_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105811.1"
                     /translation="LPLFRMADLATDGDLLAAARDDAQLILTRDPKLTTPRGEALRVL
                     LYLFERDEAVRLISAG"
BASE COUNT          889 a         1369 c         1543 g          771 t
ORIGIN      
        1 catgctggtg accgtgtcgc tgttgccgcc agtaatgaga ttgatcagga tctggattcc
       61 catgatgcgc accatctgcg cctccgggtg acggttcgcg gcatggccga tctcgtgggc
      121 cagcacgccg gccacttcct cggggcttgc cgcgcggctg atcagctcgc gggtgatgac
      181 gatgcggtcg cccggcatcg caaaggcatt cataaccggc aggtcataga ccgtgatcgc
      241 gagaggcgga agatcggcac cgctctccgc aatgcgggcc gtcattgcgg caagggccga
      301 tacgcccggc ttgcccatgc agggtcttgc cgtttcgacg attgaagcct ccatctggct
      361 accgatccgg tcgcgccagg actgcggcag atggcgggcg agtccttcgg gtgccagctg
      421 aacgacgaga tagccgccga ctgcgatcgc aacgaggtca ccggcaatcc atgccagggg
      481 gcggagcatc ctgccaagcc gcaggccgcc cctgagctgc ggagcagcct tgagaatggc
      541 attccccatg gcctcgtcat gcagaaccag ccgcgctccg ggcattgcgg cccggcagag
      601 ccggagcggc cgtcccgcgt gcggaagatc gacggcctcg agatccgaaa gcggccatat
      661 ctgttcgttc agatcctggc cgtggaagcg caacgcatcg ggcgtcagtc gcagccgcac
      721 caagatgtcg gcggccgtct tgccatcgaa atagactgcc gatccttcca tcacagcccg
      781 ccgacgtcga gggcatcggc aagaccctcg cccatggagc tgacgggtgt ccgtgattgc
      841 cggatgcggt cgatgttgac cgtgccttcg atccgcacgc ggtcgcagag gtagcgcacc
      901 aggcgctgct gcacgaaggg tgcggcgacg ccgagggtga gcacggaaag aagggcgttg
      961 ccgatggcga ggcggatgat gctgccggtg gtggcgtcga agcggaactg tgcgccatcg
     1021 aaactggtgt aggtggcgaa ctggatcagc tcgcgcgccg tgtacatggc ccagattgcg
     1081 ggatagaggg tgaggtagag cagatagaaa acggccagcg acaccacgat gaccccgatg
     1141 gcaatactga cctggtcagg ggagccgggg tcacctccct gttcgccggc gaggctggcc
     1201 cagaaaccct cgctttcgag gagggtaaac tccgcgatca gcaggccgcc cagcacagcg
     1261 atgacgagca ggctgacaaa ccagcacagc gcataggcgg ggtagagcgg gccggcaggg
     1321 cctttgaagc ggaaaggccg gtcgccgaag aaggtgctgc ccatcatctg ttgctgcagc
     1381 gtgaggttca tgaccggggt gatccagccg agcgtcatgg cgcgggccat gatgctgccg
     1441 aaatagagga gtgcataggt ggtggtggag ccgagcaggg cgccgcgtat gccgcgccac
     1501 agggtacgcg aaagctggta gcggcgagcc cggtagatgg cagcgcccca gagcaggctg
     1561 atggccagga agaccaggag ttgaagcccg atgaccgatg ggtgctcggg accgaaggcc
     1621 agggtgagcc cggcaaaggc gagcacagtg ggcaggccga agaccaggaa gacgaacatg
     1681 gcgcccttga agagctcgat tccctttccg gtgtattcca gcggctcgcc gttgatgtgc
     1741 acgcagctcc agatgtggcg ccggacattg gtgcgggccc agaagcgata gagggtgagc
     1801 gtgaggagga atagcagggc attcagcagg gacaggcgca gcaggccggt ccggggtaca
     1861 tagtggatgc gaatcgaatc gccctttgcg gcatgttccg tcatgacttc aggcgcttaa
     1921 cacaggtggt ctgaacgcct gatgaagccc ggcgagcgac cgttaagcat gcgctgtggc
     1981 ggaagtgcaa cagcagcaca aaactttatc ttgcccgatt gcacccgtct ttgaaccttt
     2041 catctgccta aatccggcaa tagaccaata aaaccgaggc gttgagtccg cagagttgcg
     2101 gcgaagcgat gcttgaatcc gggcggcggc acgccatgcg caccagtgat ccaaaggtgt
     2161 ggtggggagg tcgttggttg cccggttcta gcgcgttttg accgtggccg gggcggcgca
     2221 agcatcagga gaggatgctg aaagttgagg accgtttggt attgaccaca ccggcatgtg
     2281 acgggcgtga ttggtggatg agcgttggga ccaggccagc cgtgctggcg ctgatcgcgc
     2341 tgatcgcgct ggttgccatg tccttccgcg cccaggcggc agaggacgcc gattccgggc
     2401 cgctggccga cttctactcc tatctccccg ccacgcctga actgaagata cctgacatca
     2461 acatcccttt ctggacggat gacctcaaga agggtcagcg ggcctatcgt acgggcaagt
     2521 atgaccgggc cctgaaaagc ttccgcaagg cctcggatga aggcaatacc gtggccgact
     2581 ggtatctcgg ccacatgtac cgcctggggc agggcgtgcc ccgcaacgat gccatggcct
     2641 attcctacta ctcgcgggtg gccgactcct atgatcccga cgaaccggac cagaatcgcc
     2701 tgcgcatcgc cgtggatgcc atggtgcggc tggcggatta ccagcgcgag ggaatcggcg
     2761 aagccagcgt gaagtccaat cccgctgcgg cggcgcgcag ctatctgaag atcgcgaccg
     2821 cttatggcca cccggcggcg caatatgccc tgggcgtgat gaacatcaag ggtcagggcg
     2881 tgaagcgcaa tccgcagcag ggcctgaaat ggctgatgac ggccgcccgc aagcgctacg
     2941 cgcccgccga ggcctatctg ggcgagttgt actgggaggg caagatcgtg cggcgcgacc
     3001 ggactcgcgc cgtgatgtgg tatgcgttgg ccaaggagtc ggcccggccc gaggaaaacc
     3061 cggaaatcta tgaccgggcc ctgcagctgg aagccaatgt cagcgaagaa gaactgatcg
     3121 aggcggaggc tcgggccagg gtctgggacg gtcagtatcc ggtcgatcgc cgccgcggca
     3181 acgactgacc cgtcactgcg gcgcccggcc cggccgcgtc acgatgaagg ccgagacggc
     3241 cagaaacacc acggccactc caatctggat ccagaccggc gcctgggcgc caaaatgaag
     3301 ataggcgaac atggcggcca tcatgaccac cgcaaaaacc ttcgccttgg tgggaatgac
     3361 gcccgagtcc tgccagtcgc ggatgaaggg accgacgaca ggatgattgc ggatcttctc
     3421 ggccagttcg ggagaggacc tggaaaaggc ccagacggcc accagcagga acggcgtggt
     3481 cggaaacagc ggcatgacca ctcccagggc gcccaatccg acgcagagcc aacccgtgcc
     3541 cagcagaagc ggttttctca gcgtgccgat gacaatctcc ctgccgcgcg actgcggcgg
     3601 tttccgcctt gcgatggcct gccggttcat gccatcaacc ccatgacatg acggatccca
     3661 gcgatacacc gccgcatctg aaggcggttg atgtcgaaat caagacccag ccctccttcc
     3721 tggggcggct cagggcttat tttttcacgg ggctggtggt gacggccccc atcgccatca
     3781 caatctgggc cacctactgg ttcgtgacct tcttcgatgc ctgggtgaag cccttcatcc
     3841 ccagcgtcta caatcccgat acctatcttt cgttccaggt cccgggcttc ggcctgctct
     3901 tcgcgctgat caccatcacc atcatcggcg cgctggcggc caatctggtg ggccgcaccg
     3961 tcatcggcct gtgggacaaa ctgctcaacc gcacgccagt ggtgcgctcg atctacaagg
     4021 gctccaagca gatcttcgag acgatcttcg cgcagaaggg cgcctccttc cgctacgtgt
     4081 gcctggtgga atggccgcgg cgggactgct ggtccatcgc cttcgtctcg cgcgaggtgg
     4141 atggcgggca ggttggtctc gagccggggc gggacatgta tgcgatctat gtctcgacca
     4201 caccgaatcc gacctcgggc tatctgttct tcgccgacgt ggcggatgtg aagatcctcg
     4261 acatgagcgt ggaagatgga ttgaagctgg tgatttcgat gggcctcgtc ttccccgacc
     4321 ggccgctgcc tgcggcggag gaggcaaagg tggctgcggt caagacggcg cgtccgaagc
     4381 ggaagcctgt cagccggcgg aaatcagcct gacggcctcg tcgcgctcga acaggtagag
     4441 caggacgcgc agcgcttcgc cgcgcggtgt cgtaagcttg gggtcgcggg tgaggatcag
     4501 ctgggcgtcg tcgcgggcgg cggcgagcag gtcgccatcg gttgcgagat ccgccatgcg
     4561 gaagagcgga ag
//
LOCUS       DBNGVR010000503         5578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1957142, whole genome shotgun sequence.
ACCESSION   DBNGVR010000503 DBNGVR010000000
VERSION     DBNGVR010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1957142"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="AC16A0_10510"
     CDS             <1..1095
                     /locus_tag="AC16A0_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3105812.1"
                     /translation="PRPDRERYHAVPKFDPVTLFFLAPKPNAPSFTQVFADSLVEHAR
                     RDEKIVAITAAMPSGTGIDHFAAHFASRTFDVGIAEQHAVTLAAGLAAEGMKPVCAIY
                     STFLQRGFDQIVHDVALQRLPVRFAIDRAGLVGNDGATHAGSFDLGYMSMLPGMVMMA
                     PADAIELRRMVATAMDYDEGPIAFRYPRGETTGPDQPLEDRPLEIGRGRIMTEGTDVA
                     ILSIGSRLEACLEAARDLAHHGISATVADARFCKPLDTALVERLARSHELVITVEEGA
                     VGGFGAHVLHHLAQRDLLRHIRVRNLMLPDNFIAHGSPAEQLADAGLDSASIAREILT
                     LHTSGAIPNFPGYRRRAPHASHRNGARLHS"
     gene            1166..2182
                     /gene="bchI"
                     /locus_tag="AC16A0_10515"
     CDS             1166..2182
                     /gene="bchI"
                     /locus_tag="AC16A0_10515"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0010007 - magnesium chelatase complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR3105813.1"
                     /translation="MTARYPFSAISGQDELKQALLLTAVDPLIGGLLAFGDRGTGKST
                     AVRALAALLPKMRATTGCAYHCDPDSPDAQCATCRKQAQHKVRNIPIPVVDLPLGATE
                     DRVIGALDLERALTKGEKAFEPGLLAQANRGYLYVDEVNLLEDHIVDLLLDVAASGEN
                     VVEREGLSIRHPARFVLVGSGNPEEGELRPQLLDRFGLSVEVRTPGDVTTWVEIVKRR
                     ESFERDPTAFLAQWQKAEDKTRRRITAARKRLNDVAVPDAVLERAAALCIHLKTDGLR
                     GQLTLMRAARALAAFEGAKETCIVHLRRMAAPALRHRLRRNPLDETGSATRIDRALME
                     VFGP"
     gene            2179..3939
                     /locus_tag="AC16A0_10520"
     CDS             2179..3939
                     /locus_tag="AC16A0_10520"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit D"
                     /protein_id="HIR3105814.1"
                     /translation="MTSLPGPPPDLRSADAALAALLLAIDPIGLGGAVLRGAGGPVRD
                     EWISRFRNLLPAGSHVGKLPAYVAADRLLGGLDVPATLSAGRPMFEKGILAAADNGVL
                     ILSSAERLEADKAALIAAALDSGEVVVQRDGISSRLQARFTLIACDEGQADDEALPSA
                     LAERLAFHLDFSNMTSLDASHAGGIETLRKRMAFATVPAEISEALCTSAIALGIGSLR
                     AGLFALKAARALAALQGRSLVTAEDAAAAARLVLAPRAVRFPAADQPEEAVEPQEPDA
                     PAPDQDAENDNSSPGEMTELILEAVKPVLPKGLLEALDMAVLAQSAKSGGGALSAKAG
                     TRRGRPAGTRKGDPRNGARLNLIETLRTAAPWQRLRLKLAPGRTGIQVRREDFRINRY
                     KQRNETTAVFVVDASGSAALQRMAEAKGAVELILADCYVRRDRIALISFRGRKAEVLL
                     PPTRSLQRARRSLADLPGGGGTPLGAAIDQAALIADQVRRGGGKPLLVFLTDGRGNIA
                     RDGTADRAAALRDAAEAARALRVQGFRAMVIDISATPSDAARQLAGNMAATYLPLPHA
                     DASRISRSVSTAMKAGGTSA"
     gene            3936..4820
                     /gene="bchO"
                     /locus_tag="AC16A0_10525"
     CDS             3936..4820
                     /gene="bchO"
                     /locus_tag="AC16A0_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase BchO"
                     /protein_id="HIR3105815.1"
                     /translation="MIAHLDWARDGADWPNRDASRFVDAAGMVWHVQIMGQGPVILLL
                     HGTGASTHSWRDILPKLADRFTVVAPDLPGHGFTADPPASAQSLPGMASAVADLLRKL
                     GLMPFRSVGHSAGAAVLVRMTVRKMIAPDDIVSFNGAFFPVSGVAGHFFTPLAKAAAA
                     TSFMPKLFASMADQKAVERLLNDTGSKIDARGLDLYRRLFANQGHVAGTLRMMAAWDL
                     HWTEQDLRNLTCRLYLVRGAADRTISPRDSDRAAKLCPTASVITLANLGHLAHEENPG
                     LAAEIIADPEGYLLRNSN"
     gene            4840..>5578
                     /gene="crtI"
                     /locus_tag="AC16A0_10530"
     CDS             4840..>5578
                     /gene="crtI"
                     /locus_tag="AC16A0_10530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3105816.1"
                     /translation="MFTDTDQASDRRPHAAVIGSGLGGLAAAIRLGAKGYRVTVFEKL
                     EQPGGRAGVFRQDGFTFDAGPTIITAPFLLEELWSLAGCKLADDVELRALDPFYAIRF
                     DDGSVFTCCGDPARMRAEVGRLSPGDVAGYDRFMAESEAIYRIGFEQLGHVPFGSPMD
                     MLRILPDLMKLGAWRTVHQHVAARVKDPRIRMALSFHPLFIGGNPFRVTAIYSMIAYL
                     EREFGVHAAMGGTNRIVSGMAKLIRGQG"
BASE COUNT          907 a         1970 c         1765 g          936 t
ORIGIN      
        1 ccgagacccg accgggagcg ctaccacgcc gttcccaaat tcgatcccgt caccctgttc
       61 ttcctcgcgc ccaaacccaa tgcgccgagt ttcacgcagg tcttcgcgga cagcctcgtg
      121 gagcacgccc gccgtgacga gaagatcgtg gcgatcaccg ccgccatgcc gtccggcacc
      181 gggattgacc atttcgccgc gcatttcgcc tcccgcacat tcgatgtcgg catcgccgag
      241 cagcatgcgg tgacgctggc cgccggtctt gcggcggagg gcatgaagcc ggtctgcgcc
      301 atctattcca ccttcctgca gcgcggcttc gaccagattg tccatgacgt ggccctgcag
      361 cgcctgcccg tgcgcttcgc gatcgaccgc gccggcctcg tgggcaatga cggggccacc
      421 catgcgggtt cctttgatct gggctacatg tcgatgctcc cgggcatggt gatgatggca
      481 ccggctgacg ccatcgaact gcgccgcatg gtggcgacag ccatggacta tgatgaaggc
      541 cccatcgcct tccgctatcc gcgtggcgag accaccgggc cggaccagcc cctcgaggat
      601 cgcccactcg agatcggccg ggggcgcatc atgaccgagg gtaccgatgt ggcgattctc
      661 agcatcgggt cgcggcttga ggcctgcctc gaggccgcgc gcgatcttgc ccaccacggc
      721 atctctgcca cggtcgccga tgcgcggttc tgcaagccgc tcgatacggc tctggtggag
      781 cggctggctc gcagccatga attggtgatc actgtggaag aaggcgcggt tggcgggttt
      841 ggcgcccatg tcctgcacca tcttgcccag cgcgatctcc tgcgccacat ccgggtacga
      901 aacctgatgc tgcccgataa cttcattgcc cacggatctc cggccgagca gcttgccgat
      961 gctggcctcg acagcgccag catcgcgcgc gagatcctga cccttcacac cagtggcgcg
     1021 atcccgaact tccccggata ccgccgccgc gcgccccacg cgtctcacag gaatggcgcg
     1081 cgcctgcatt cgtgatgaca tgtcgacatt acaggcggac cgttccatgt caggatggct
     1141 catcaaggcc tgaacaggac gtcacatgac tgcgcgctac ccgttttccg ccatctccgg
     1201 ccaggacgag ctgaagcagg ccttgctgct gactgctgtc gatccgctga tcggaggcct
     1261 gctggccttt ggcgatcgcg gcaccggcaa gtcgacagcc gttcgtgccc tggccgccct
     1321 gctccccaag atgcgcgcca ccaccggctg cgcctatcac tgcgatcccg actctcccga
     1381 tgcccaatgc gcgacctgcc gcaagcaggc gcagcacaag gtgcgcaaca tccccatccc
     1441 cgtcgtcgac ctgccgctgg gcgcaaccga agacagagtc atcggcgcgc tcgacctcga
     1501 gcgtgccttg accaagggcg agaaagcctt cgaacccgga ctgctggccc aggccaatcg
     1561 cggctatctt tacgtcgacg aagtgaactt gctcgaggac catatcgtcg acctgctgct
     1621 cgacgtcgcc gcctcgggcg agaacgtggt ggaacgcgag ggtctcagca tccgccatcc
     1681 ggcgcgcttc gtgctggttg gcagcggcaa tccagaagaa ggcgagctcc gcccgcaact
     1741 tcttgatcgt ttcggactct ccgtcgaggt tcgcacaccg ggcgacgtga ccacctgggt
     1801 ggaaatcgtc aaacgccgcg agtccttcga acgcgatcca accgcctttc tcgcccagtg
     1861 gcagaaggcc gaggacaaga cgcggcggcg cattaccgca gcgcgcaagc gactcaatga
     1921 cgtcgccgtt cccgacgccg ttctggagcg ggcggccgcg ctgtgcatcc acctcaagac
     1981 tgacggtctg cgcggccagc tcacgctgat gcgcgcggcc cgcgcgcttg ccgccttcga
     2041 aggggccaag gaaacctgta tcgttcactt gcgccgcatg gcggctcccg ccctgcgcca
     2101 tcgcctgcgc cgcaacccgc tggacgaaac cggatcggcg acccgcatcg accgcgccct
     2161 gatggaagtc ttcggcccat gacctccctg cccggcccgc cgcccgatct ccggtcggcc
     2221 gatgcggcat tggcggccct ccttcttgcc atcgatccga tcggcctggg cggtgccgtg
     2281 ctgcgcggcg cgggcggtcc ggtaagggat gaatggatca gccgcttcag gaatctgctg
     2341 cccgccggct cccatgtcgg caagcttcct gcctatgtgg ccgcggacag attgctgggg
     2401 gggctcgatg tgcccgcaac cctcagcgcg ggccgcccca tgttcgagaa gggtatcctc
     2461 gcggctgccg acaacggggt tctgatcctc agctccgccg agcggctcga ggcggacaag
     2521 gcggccctga ttgcagcggc actggacagc ggtgaagtgg tcgtccagcg cgacgggatt
     2581 tcatcccggt tgcaggcccg tttcaccttg atcgcatgcg acgaaggcca agccgacgat
     2641 gaagcccttc cgtccgcact ggctgaacgg ctggcctttc acctcgactt ctcgaacatg
     2701 acgtcactcg acgccagcca tgccggcgga atcgagacgc tccgtaaacg gatggctttc
     2761 gccacggttc cagcagagat atccgaggcg ctctgcacca gtgcgattgc gctgggcatc
     2821 ggcagcctgc gtgccggcct cttcgcgctg aaggctgcga gagcgctggc agccttgcaa
     2881 ggccgcagcc tggtgacggc agaggatgcc gccgccgccg cccgtctcgt cctggccccg
     2941 cgggccgtcc gctttccggc cgccgaccag ccggaggaag ccgtcgaacc gcaggaaccc
     3001 gatgcccctg caccggacca ggatgcggag aacgataact catcgccggg cgaaatgacg
     3061 gaactcattc tcgaagcggt aaagcccgtt ctccccaagg gattgctgga ggccctcgac
     3121 atggcggtgt tggcgcagtc cgcgaagtcc ggcggcggcg ccctgtcggc caaggcagga
     3181 acgcggcggg gccggccggc aggcacgcgc aagggcgacc cacgcaacgg cgcccgcctc
     3241 aatctcatcg agactctgcg gacggcggca ccctggcaaa ggctcaggct gaagctggcg
     3301 ccgggccgca ccggcataca ggtgcggcgc gaggacttcc gcatcaaccg ttacaagcag
     3361 cgcaacgaga ccacggcggt cttcgtggtc gacgcatcgg gttcggccgc cctgcagcgc
     3421 atggcggaag ccaagggcgc cgtcgagctg atccttgccg actgctacgt ccggcgtgac
     3481 cgcatcgccc tcatctcttt ccgcggccgc aaggccgaag tgcttctgcc tccgactcgc
     3541 tcgctgcagc gcgcccgccg cagcctcgcc gatcttcccg gcggcggtgg aacgcccctc
     3601 ggtgcggcca tcgatcaggc cgccctcatc gccgaccagg tgcggcgcgg cggcggcaag
     3661 ccgctgctgg tgtttctcac cgacgggcgc ggcaacatcg cgcgcgacgg cacagccgat
     3721 cgcgcggctg cgctccgcga tgccgcggaa gcggcaagag cgctgcgcgt tcaagggttc
     3781 cgcgccatgg tcatcgacat ctccgccacg ccgagcgacg ccgcacggca actggcgggc
     3841 aacatggccg caacctatct gccacttcca catgccgatg ccagccgcat ctcgcgctcg
     3901 gtatcgaccg ccatgaaggc aggcgggacc tcggcatgat cgcccatctc gactgggcgc
     3961 gcgacggcgc cgactggccg aaccgcgacg ccagccgttt cgtcgacgcc gcgggaatgg
     4021 tctggcatgt gcagatcatg gggcaagggc ctgtcatcct gctcctgcac ggcaccggag
     4081 cctcgacgca ttcgtggcgc gacatcctgc cgaagctggc cgaccgcttc acggtggtgg
     4141 cgcccgatct gcccggccac ggattcacgg ccgatccgcc ggcctccgcg cagtcgctgc
     4201 cgggcatggc cagcgcggtc gcagatctgc ttcgcaaact cggcctcatg ccgttccgca
     4261 gcgtcgggca ttcagccggc gcggcggtcc tggtgcgcat gaccgtgcgc aagatgatcg
     4321 cgccggacga catcgtcagc ttcaatggcg ccttcttccc ggtgagcggg gtggccgggc
     4381 atttcttcac gccgctggcc aaggccgcgg ccgccaccag cttcatgccg aagctctttg
     4441 cctcgatggc ggaccagaag gcagtcgagc gcctgctcaa tgacaccggt tcgaagatcg
     4501 atgcacgcgg cctcgacctt taccggcggc tcttcgcgaa ccagggtcac gtcgccggca
     4561 cattgcggat gatggcggcc tgggacctgc actggaccga acaggatctg cgcaacctca
     4621 cttgccgcct ctatctcgtg cgcggcgctg ccgaccgtac catctcgcca cgcgatagcg
     4681 accgggccgc aaagctctgc ccgaccgcca gcgtcatcac cttggcaaac ctcggacatc
     4741 tggctcacga ggagaatccc ggcctggcgg cggagatcat tgccgacccg gaaggctacc
     4801 tcttgcgcaa ttcgaattga cacggtctaa tgacaggaca tgtttacaga tactgaccaa
     4861 gcctcggacc gaaggcccca tgcggccgtc atcggcagtg gtctgggcgg gctggccgcc
     4921 gccatccggc ttggcgccaa gggctatcgc gtcacggtgt tcgaaaagct cgagcagccc
     4981 ggcgggcgcg ccggtgtgtt ccggcaggat ggcttcacct tcgatgccgg ccccaccatc
     5041 atcaccgcgc cgttccttct cgaggaactg tggtcgctcg cgggatgcaa gctcgccgac
     5101 gatgtggaac tgcgcgcgct cgatcctttc tatgcgatcc gcttcgacga cggctcggtc
     5161 ttcacctgct gcggcgatcc cgcgcggatg cgcgccgagg tgggccggct gtctcccggt
     5221 gacgtggcgg gctacgaccg attcatggca gagagcgaag cgatctaccg gatcggcttc
     5281 gaacagcttg gccatgtgcc cttcggctcg ccgatggaca tgctgcgcat cctgccggac
     5341 ctgatgaagc tgggcgcctg gcgcaccgtg caccagcatg tggccgcgcg cgtgaaggac
     5401 ccgcgcatcc gcatggcgct gagcttccat cccctcttca tcggcggcaa cccgtttagg
     5461 gtcaccgcca tctacagcat gatcgcttat ctcgagcgcg agttcggcgt gcatgccgcg
     5521 atgggcggaa ccaatcgcat cgtgagcggc atggcgaagt tgatccgcgg ccagggcg
//
LOCUS       DBNGVR010000504         2416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4117559, whole genome shotgun sequence.
ACCESSION   DBNGVR010000504 DBNGVR010000000
VERSION     DBNGVR010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4117559"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..955
                     /locus_tag="AC16A0_10535"
     CDS             158..955
                     /locus_tag="AC16A0_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3105817.1"
                     /translation="MKPLSSPSPHIQAFVEDSIGWLVLNRPERRNALTGAMWAAIPPL
                     MKSLDEQADVRAIVLRGQGREAFAAGADISEFGEARNHAAAAAAYEALNGAAFAAIRG
                     ARRPVIAMIQGFCIGGGLAIALACDLRVADASALFALPPSRLGLAYPLDGLRDLVATV
                     GAPMAKEMLFTARRLKAEEALARGLINRLAADAESETRRLCGEIAGGAPLSILHAKRA
                     IDLIAARPGHETSAEVAARAAACFNSADYAEGRRAFAEKRTPAFEGR"
     gene            complement(960..1706)
                     /locus_tag="AC16A0_10540"
     CDS             complement(960..1706)
                     /locus_tag="AC16A0_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3105818.1"
                     /translation="MHKFNSNGVEIAYETAGDGPPILLIHGFASTGRVNWWDTGWVKT
                     LTEAGRQVITFDNRGHGASQKLYESRLYPATEMAEDARRLLDHLGIVQADVMGYSMGA
                     RVAAFLTISHPERVRRAVFAGLASRMITGVGGGEEIAQALEAASRDEVTDPGARSFRI
                     FAEQTRSDLTALAACIRSSREKITLGELRGITVPVLVVAGDKDDVAGDVDTLVAAIPG
                     ARGVVLPNRNHMNAVGDRGYKDSVLAFLAD"
     gene            1759..2001
                     /locus_tag="AC16A0_10545"
     CDS             1759..2001
                     /locus_tag="AC16A0_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HIR3105819.1"
                     /translation="MASGATPHFQNEMGLASIEIGARTFMCIGAKPPFDHPHVFIDMG
                     TADEIVCPYCSTLYRFNARLRPDQSTPEQAAWNDAA"
     gene            2066..>2416
                     /locus_tag="AC16A0_10550"
     CDS             2066..>2416
                     /locus_tag="AC16A0_10550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3105820.1"
                     /translation="MQRHERRFDERVKPPQEIGDWYIRELLASVEKHKGRLLVAEVDG
                     VVAGYACLLVEVSSADQREEILYTYSQVSDLAVLSAHRGHGLGRALIAACEGIARADG
                     QRWIRLGVLAANQDA"
BASE COUNT          413 a          796 c          778 g          429 t
ORIGIN      
        1 cggatgcggt cccagaccgg atcgagcttc tcgacggttt tcgctgcctg ccgcatcgcg
       61 cactcccatg tcgagatgaa gtatatccct aaagtgccga aatggggaga gtggcagccg
      121 attgtgatag cgcccggtta acgctacaag cgcggccatg aaacccttgt cctcgccttc
      181 cccccatatt caggcttttg tcgaagattc aatcggttgg ctggtgttga accggcccga
      241 acggcgcaat gcgctgaccg gcgccatgtg ggccgccatt ccgccattga tgaaatctct
      301 tgatgagcag gctgatgtga gggcgatcgt gctgcgcggc cagggccgcg aggccttcgc
      361 cgccggggcc gacatttcgg agtttggcga agcccggaac cacgcggcgg ccgcggcggc
      421 ctatgaagcc ctgaacggtg cggcctttgc cgcgatccgc ggcgcgcggc ggccggtgat
      481 cgccatgatc caggggttct gcatcggcgg cggactggcc atcgccctgg cctgcgacct
      541 gcgggtggct gacgcctccg cgctcttcgc gttgccgcct tcgcgcctgg gcctcgccta
      601 tccgctggat ggcctgcgcg acctggtggc cacggtggga gcccccatgg ccaaggagat
      661 gctgtttacc gcccgccgcc tgaaggcgga ggaggctctg gcgagaggtc tgatcaaccg
      721 gctcgcggcg gatgcagagt ccgagacgcg aaggctctgt ggcgagatcg ccggcggtgc
      781 gcccttgtcg atccttcacg ccaagcgcgc catcgacctg attgccgcac ggcccgggca
      841 tgagacgtct gccgaggttg ccgcacgggc tgccgcctgc ttcaacagcg ccgactatgc
      901 cgagggacgc cgagccttcg cagaaaagcg gacaccggca ttcgagggcc gctgacggtt
      961 cagtccgcaa ggaacgccag caccgagtcc ttgtagccgc gatcaccgac ggcattcatg
     1021 tgattgcggt ttggcagcac gacacccctg gcgcctggaa tggctgcaac caaggtatcg
     1081 acgtcacccg ccacgtcatc cttgtcgccc gccaccacga ggaccggcac agtgatgcca
     1141 cgcagctcgc ccagcgtgat cttctcgcgc gacgacctga tgcaggcggc aagtgccgtg
     1201 agatcgctgc gggtctgttc cgcaaagatg cggaagctgc gcgcgccggg atctgtcacc
     1261 tcgtcgcggg atgcggcttc cagcgcttgc gcaatctcct cgccgccgcc cacgccggtg
     1321 atcatccggg aagcaagacc ggcgaacacg gcacgccgca cgcgctcggg atggctgatt
     1381 gtcaggaagg ccgccacccg cgctcccatc gaatacccca tcacatcggc ctggacgatt
     1441 ccaagatgat cgagaagccg ccgtgcgtct tcggccatct cggtcgcagg atagagccgc
     1501 gattcataga gcttctggct ggcgccgtgg ccgcgattgt cgaaggtaat cacctggcgg
     1561 ccggcctcgg tcagggtctt gacccatccc gtgtcccacc agttcacccg gcccgtggaa
     1621 gcaaagccgt ggatcagaag gatgggcggt ccgtcgcccg cagtctcgta ggcgatctcg
     1681 acgccgttgg aattgaattt gtgcatcacc gcgaacatat gctaatcgcc ccgcgagaca
     1741 agatcgtcga ggttcatcat ggcatcgggc gcaacaccgc acttccagaa cgagatgggg
     1801 ctggcctcca tcgagatcgg cgccaggacg ttcatgtgca tcggggccaa gccgcccttc
     1861 gaccatccgc atgtcttcat cgacatggga acggcggacg agatcgtctg cccctattgc
     1921 tccacgctct atcgcttcaa cgcccgcctg cggccggatc agtcaacgcc tgagcaggcc
     1981 gcctggaacg acgctgcctg acccctgcac aattcgcgac taccgggacg cggatcaggc
     2041 tgccgtcctc gcgatcattc gcgatctgca gcgtcacgag cgccggttcg acgagcgggt
     2101 gaagccaccc caggagatcg gcgactggta catccgggag cttctggcct ctgtcgagaa
     2161 gcacaagggc aggctgctcg tcgcggaggt ggatggcgtg gtcgccggct atgcctgcct
     2221 gctcgtcgaa gtctcttcgg ccgatcagcg cgaggagatc ctctacactt acagtcaggt
     2281 ctccgatctg gccgtcctca gcgcccatcg cgggcacgga ttgggccggg ctctcattgc
     2341 ggcatgcgaa gggatcgcgc gcgcggatgg gcagagatgg attcggctcg gtgtgcttgc
     2401 cgcgaaccag gacgca
//
LOCUS       DBNGVR010000505         1538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_386093, whole genome shotgun sequence.
ACCESSION   DBNGVR010000505 DBNGVR010000000
VERSION     DBNGVR010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_386093"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16A0_10555"
     CDS             <1..335
                     /locus_tag="AC16A0_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR3105821.1"
                     /translation="GDMFLDVVNAADKQYFVAKSSILRVELVEVPKASQMNLQRRATD
                     RDRFDPWDVLGVAQGSDGETIRQAYHAKIRAYHPDRLAGLDLPREMKDYAAAMVVRIN
                     LAYEQIGT"
     gene            complement(339..1271)
                     /locus_tag="AC16A0_10560"
     CDS             complement(339..1271)
                     /locus_tag="AC16A0_10560"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3105822.1"
                     /translation="MQLFPTPDNPLPDGAVCVALQTEDGIRLRAMRAGPRQARGTVVI
                     LGGRGDFMERYFETASDLIARGYHVASVDLRGQGGSERLLDDPLRSHVTSFKGYDTDV
                     RTLMRDLVMPHCPAPHFALGHSTGGHVLLRLLRRDKFFARVVVCSPLLGILYGRWPRP
                     AAALLVFLACSLGLGWLYLPGQRKRPLGRSDFGVYPLTSDRRRWQRDSGILEVTPQLG
                     VGAPTFGWLRAARRSVAALRWLGRHSRLTAPVLILAAGADRIVSNAAIKAFAARVPGV
                     ALCIIPGAQHELLAENSAIRQEVLAAIDSYFGTA"
     gene            complement(1326..>1538)
                     /locus_tag="AC16A0_10565"
     CDS             complement(1326..>1538)
                     /locus_tag="AC16A0_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105823.1"
                     /translation="PKAESTAAKAAGGALSCDKAGQIISGYGFSGVKPSVCTGKVYAF
                     NATRDGNSFAIKLDSGSGELTEVKKQ"
BASE COUNT          275 a          507 c          513 g          243 t
ORIGIN      
        1 acggtgacat gttcctcgat gtcgtcaatg ccgccgacaa gcagtacttc gtggccaagt
       61 ccagcattct gcgtgtcgag ttggtggaag ttcccaaggc cagccagatg aacctgcagc
      121 gccgcgccac cgatcgcgac cgtttcgatc cctgggatgt cctgggtgtg gcccagggct
      181 cggatggcga gacgatacgc caggcctatc acgccaagat cagggcctat cacccggacc
      241 gtctggcagg gctcgacctg ccccgggaga tgaaggatta tgccgccgcc atggtggtgc
      301 ggatcaacct cgcctacgag cagatcggca cctgagcgtc aggccgttcc aaagtaactg
      361 tcgatcgcgg ccaggacttc ctgccggatc gcgctgttct cggccagcag ttcgtgctgg
      421 gcgcccggaa tgatgcagag cgccacgccc ggaacacgcg cggcgaaggc cttgatcgcc
      481 gcattgctga cgatcctgtc ggcgccggcg gcaaggatca gcaccggagc tgtcaggcgg
      541 ctgtgccggc caagccagcg gagtgctgcc accgatcgcc gcgccgcgcg cagccagccg
      601 aaggtgggcg cgccgacccc aagctgcggc gtgacttcca ggatgccgct gtcgcgctgc
      661 cagcgccgcc ggtccgaggt gagcggatag acgccgaagt cgcttcggcc cagggggcgc
      721 ttgcgttggc ccggcaggta gagccagccc aggcccaggc tgcaggccag gaagaccagc
      781 aatgccgccg cgggcctcgg ccacctgccg tacagaatgc cgagcagggg agagcacacc
      841 accacccgtg cgaagaactt gtcacggcgc aagaggcgga gcagtacatg gccgccggtc
      901 gagtgtccca gtgcaaaatg gggcgccggg caatgcggca tgaccagatc gcgcatcagg
      961 gtgcgcacat cggtatcata gcccttgaaa ctggtgacat ggctgcgcag cggatcgtcc
     1021 agcaggcgct ccgaaccgcc ctggccgcgg aggtccaccg atgccacatg gtaaccgcgc
     1081 gcgatgagat cgctcgccgt ttcgaaatag cgctccatga agtcgccacg accgcccagg
     1141 atgacgacag tgccgcgcgc ctggcgcgga ccggcccgca tggcgcggag ccggatgccg
     1201 tcctcggtct gaagggccac gcacacggcc ccgtccggga gcggattgtc cggcgtggga
     1261 aagagctgca ttgcaaatct ccggcttgcg tgccggttta ccgccgggcg ccgccgacgg
     1321 acaagctatt gcttcttcac ttcggtcagt tcaccgctgc ctgagtccag cttgatggcg
     1381 aagctgttgc catcgcgggt ggcattgaag gcatagactt tgccggtgca gaccgaaggc
     1441 ttgacgccac tgaagccgta gccagagatg atctgccccg ccttgtcgca gctcagggca
     1501 ccgcccgcag ccttggctgc cgtcgattcc gccttggg
//
LOCUS       DBNGVR010000506         4026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3136260, whole genome shotgun sequence.
ACCESSION   DBNGVR010000506 DBNGVR010000000
VERSION     DBNGVR010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3136260"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC16A0_10570"
     CDS             complement(<1..407)
                     /locus_tag="AC16A0_10570"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR3105824.1"
                     /translation="MIRFPPFGDGQRIGLFGGSFNPAHYGHYMVALYALKKLQLDWVW
                     WLVSPQNPLKDPSETGEYGARLDFTRRIARHPRFVVTDIEKQLGSRTTAETLRGMAPV
                     LKRAHFVWIMGADSFANLHHWHDWLEIPGTLPLA"
     gene            complement(404..1675)
                     /locus_tag="AC16A0_10575"
     CDS             complement(404..1675)
                     /locus_tag="AC16A0_10575"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR3105825.1"
                     /translation="MNAVTSNREMMMTLGQAARIAARELARAPSAQKDEALWAMAAKI
                     RRSAADIAAENRRDLENARARELKPSFVDRLTLDGIRIEALAKGLEDIAALPDPVGAV
                     MAHWTRPNGLDFSRVRVPIGVIGIIYESRPNVTADAGGLCMKSGNAAILRGGSDSFHS
                     SAAIVACLQRGLDVAGLPRTAVQMVPTADRDCVGLMLEGLGGTLDLIVPRGGKSLVAR
                     VQSEARVPVFSHLEGICHVYVDKAADTDMACRIVLNSKMRRTGICGAAETVLVDRAGA
                     ERHLRPVIRTMIDAGCEVRGDVHAMAADPRVKPASEADWSAEYLDAVVAVKVVDGVGE
                     AIDHITRYGSQHTDSIVTDDLAAAERFLAELDSAIVLHNASTQFADGGEFGFGAEIGI
                     ATGKLHARGPVGLEQLTTFKYQVRGTGQVRP"
     gene            complement(1672..2799)
                     /gene="proB"
                     /locus_tag="AC16A0_10580"
     CDS             complement(1672..2799)
                     /gene="proB"
                     /locus_tag="AC16A0_10580"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR3105826.1"
                     /translation="MTSRLAKARRIVVKIGSALLVDGKTGAIKASWLSSLIDDLADLR
                     VGGAEIIIVSSGAIALGRRSLGLSKGKLKLEQSQAAAAVGQIALAQAWSEALRTRNMV
                     AAQVLVTLTDTEERRRYLNARATLATLLDQGAVPVINENDTVATSEIRYGDNDRLAAR
                     VASMMSADCLVLLSDIDGLYSAPPGSKGARFIPEVPAITSEIEAMAGKPISGLGSGGM
                     ITKIEAGKIALGAGCNMVIASGHERHPLRRVVDGARCTWFLASASALQSRKRWIAGTL
                     QPTGRLVVDDGAVAALSKGKSLLPAGVRRVDGSFTRGDTVSIVTARGHEIARGLVAYD
                     AGDAARIAGLKSGEIEKVLGFRGRDELVHRDDMVLMVGELT"
     gene            complement(2796..3839)
                     /gene="obgE"
                     /locus_tag="AC16A0_10585"
     CDS             complement(2796..3839)
                     /gene="obgE"
                     /locus_tag="AC16A0_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR3105827.1"
                     /translation="MKFLDQAKVYVRSGNGGAGAVSFRREKFIEMGGPDGGDGGKGGD
                     IWVEAVDGLNTLIDYRYQQHFKAATGGHGMGRLRAGANSADIVLKVPAGTEILEEDNE
                     TVIADLAKAGDRVRIAKGGNGGFGNAYFKAATNRAPRHANPGQEGEERWIWLRLKLIA
                     DAGLVGLPNAGKSTLLAAVSAAKPKIADYPFTTLHPQLGVVRAAGNSFVLADIPGLIE
                     GAHEGHGLGTRFLGHVERCAVLLHLIDVTGEDPVAAYRVIRKELKAYSPLLAEKDEII
                     AFNKIDAVSEEDLTAKLADFRRRVRKTPLLISGATGKGVDDAMKKLLTIIENAKALAR
                     KDQPGVTSEEWRP"
BASE COUNT          695 a         1358 c         1259 g          714 t
ORIGIN      
        1 gcgagcggga gtgttccggg aatctccagc cagtcgtgcc aatggtgcag gttggcgaag
       61 ctgtcggcgc ccatgatcca cacgaaatgt gcacgcttca gcaccggcgc catcccgcgc
      121 agggtctctg cagtggtgcg cgatcccagc tgcttctcga tatccgtcac gacgaagcgc
      181 ggatggcgcg caatcctgcg ggtgaagtcg aggcgggcgc catactctcc ggtttcagaa
      241 ggatccttca gcggattctg cggcgatacc agccaccaga cccagtccag ctgcagcttc
      301 ttcagcgcat agagcgccac catgtagtgc ccgtagtgcg ccggattgaa ggatccgccg
      361 aagaggccga ttctctgtcc gtcgccgaag ggtggaaagc ggatcaaggc cggacctgac
      421 ccgtgccccg cacctggtac ttgaaggtgg tgagctgttc gagaccgacc ggcccccgcg
      481 catgcagctt gccggtggcg atgccgatct ccgcgccaaa cccgaattct ccgccatcgg
      541 caaactgcgt cgacgcattg tgcagcacga tggcgctgtc gagctccgcg aggaaccgct
      601 cggccgccgc caggtcatcg gtcacgatcg aatcggtgtg ctgcgatccg tagcgcgtga
      661 tgtggtcgat ggcttcgcca acgccatcga ccaccttcac ggcgacaacg gcgtcgagat
      721 actccgccga ccagtccgct tcgctggcag gcttgacgcg cggatcggcg gccatcgcat
      781 ggacatcgcc gcgcacctcg catccggcat cgatcattgt acggatcacc ggcctgagat
      841 ggcgttcggc acccgcccgg tccaccagga cggtttccgc cgcaccgcag atgcccgtgc
      901 gccgcatctt ggagttgaga acgatgcggc aggccatgtc ggtgtcggcc gccttgtcga
      961 cataaacatg gcagatgcct tcgaggtggc tgaagaccgg cacgcgcgcc tcggactgca
     1021 cgcgtgccac caggctcttg ccgccgcgcg gcacgatgag gtcaagcgtg ccgcccaggc
     1081 cctccagcat gagtccgacg cagtcgcgat cggcggtcgg caccatctgg accgcagtgc
     1141 gcggcaagcc ggcaacatcc agtccgcgtt gaaggcaggc cacgatggcg gccgatgaat
     1201 gaaagctgtc cgagccgccg cgcaggatcg cggcgttgcc cgacttcatg cacagccctc
     1261 cggcatcggc cgtcacgttg gggcggcttt cgtagatgat gccgatgaca ccgatgggaa
     1321 cccttacccg cgagaaatcc aggccattgg ggcgagtcca atgcgccatc accgcgccga
     1381 ccggatcggg aagtgccgcg atgtcttcga gacccttcgc aagcgcttcg attcgaatgc
     1441 cgtcaagagt cagcctgtcg acaaagcttg gcttaagttc gcgcgcccgc gcattctcaa
     1501 ggtcgcgcct gttctcggcg gcgatgtctg ccgccgagcg ccggatcttg gctgccatgg
     1561 cccacagcgc ctcgtccttc tgggcggagg gtgcacgtgc cagctcgcgt gcggcgatgc
     1621 gcgccgcctg gcccagcgtc atcatcatct cgcgattgct ggtcacggcg ttcatgtcag
     1681 ctctccaacc atcagcacca tgtcgtcgcg gtgcaccaac tcgtcgcggc cccggaaacc
     1741 cagaaccttt tcgatctccc cggacttgag gcctgcaatg cgcgccgcat cgcccgcatc
     1801 ataggcgacc agcccacgcg cgatctcgtg tccgcgtgcg gtcacgatac ttaccgtatc
     1861 gccgcgcgtg aagctgccat cgacgcggcg cacgccggcc ggcaacagac tcttgccctt
     1921 cgagagcgcc gcgacggcgc catcgtcgac cacgagacgg ccggtcggct gcaaggtgcc
     1981 ggcaatccag cgcttgcgtg actgcagcgc cgaggcggag gcgagaaacc aggtgcagcg
     2041 cgcgccatcc accacgcgcc gcaacggatg gcgctcatgg ccggaggcga tcaccatgtt
     2101 gcagccggcg cccagcgcga tctttcccgc ctcgatcttg gtgatcatgc cgccggagcc
     2161 caggcccgag atcggcttgc ccgccatggc ctcgatctcg gaggtgatgg ccggaacttc
     2221 ggggatgaac cgggcgccct tgctcccggg cggagcgctg tagagcccat cgatgtccga
     2281 cagaagcaca aggcaatcgg ccgacatcat ggaggccacg cgggccgcca gccggtcatt
     2341 gtcgccatag cggatctcgc tcgtcgccac ggtgtcgttt tcgttgatga caggtacggc
     2401 gccctggtcg agcagcgttg ccagtgtggc gcgggcattg agatagcgtc ggcgctcttc
     2461 ggtgtcggtg agcgtcacca gcacctgagc ggccaccatg ttgcgggtgc gcagcgcctc
     2521 ggaccaggcc tgggccagcg caatctggcc cacggcagcc gcggcctggc tctgctccag
     2581 cttcagcttg cccttggaaa ggccgaggct gcggcgcccc agtgcgatgg cgcccgacga
     2641 gacgatgatg atctcggcac cgccgacgcg caggtcggca agatcatcga tcagcgacga
     2701 cagccatgag gccttgatcg cccctgtctt gccgtcgaca agcagggccg atccgatctt
     2761 caccacgatg cggcgggcct tggcaaggcg cgatgtcacg gccgccactc ctcggaagtg
     2821 acgccgggct gatccttcct ggcgagcgcc ttggcattct cgatgatggt gagcagcttc
     2881 ttcatggcat cgtcgacgcc cttgccggtg gcacccgaga tcagcagtgg cgtcttgcgg
     2941 acacgcctcc tgaagtcggc cagcttcgca gtgagatctt cctccgaaac tgcgtcgatc
     3001 ttgttgaagg cgatgatctc gtccttctcg gcaaggagcg gcgagtaggc cttgagttcc
     3061 ttgcggatca cgcgataggc ggccaccgga tcctcaccgg tcacatcgat gaggtgaagg
     3121 agcaccgcgc agcgttcaac gtggccaagg aagcgcgtgc ccagtccatg cccctcgtgt
     3181 gcaccttcga tgaggccggg aatgtcagcc agcacgaagc tgtttccggc ggcccgcacg
     3241 acgcccagct gcggatgcaa ggtggtgaag ggatagtccg cgatcttcgg cttggccgcg
     3301 gaaacagcgg ccagcagcgt cgacttgccg gcattgggaa gcccgacaag gccggcatct
     3361 gcgatgagct tgaggcgcag ccagatccac cgctcctcgc cctcctggcc aggattggca
     3421 tggcgcggcg cgcgattggt ggccgccttg aaataggcat tgccgaagcc gccattgcca
     3481 cccttggcaa tgcgcacgcg atcacctgcc ttggcgagat cggcgatgac ggtctcgttg
     3541 tcttcctcga ggatctcggt accggcggga accttgagca cgatgtccgc cgaattggcg
     3601 cccgccctga gccggcccat gccatgtcca ccggtcgccg ccttgaaatg ctgctgatag
     3661 cggtagtcga tgagcgtgtt gaggccgtcg acggcctcga cccagatgtc gccgcccttg
     3721 ccgccatcgc ctccgtcggg cccacccatt tcgatgaact tttccctacg gaatgagacc
     3781 gcgcccgctc cgccattgcc ggagcgcaca taaacctttg cctggtcgag gaatttcatc
     3841 ttccagcctc atatcctttc gaggccccga cgcctagccc cccgtggcgc ggcagccaca
     3901 atcggcaatc gtcttcgcat tgcaccattt tccccacatt ggagacacat ttgggaagtc
     3961 tatccccggc cttcacccga acatcggcga tcggttgaac ctgaagccca gtcaaacggt
     4021 attcag
//
LOCUS       DBNGVR010000507         4116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4905698, whole genome shotgun sequence.
ACCESSION   DBNGVR010000507 DBNGVR010000000
VERSION     DBNGVR010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4905698"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="AC16A0_10590"
     CDS             <1..810
                     /locus_tag="AC16A0_10590"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HIR3105828.1"
                     /translation="RGGIVHLKRRQQRGQTAGVITATRGNHGQSIPFGAARVGIPVTV
                     VVPHGNSVEKNAAMRALGAELIEVGHDFDAAREAAARIAAERGLDFVPSFGPDLVVGV
                     STYAHELFRAAGALDAVYVPIGMGSGICGVIAARDMLGLRTKVIGVAAAGANAYALSF
                     ATGKPVSTNSAATFADGMAVRIPNADAFHVIKHGAERIVTVSDDEIAEAVRLYFRAAH
                     TIAEGAGAAALAALMQERDRMKGKRAGVILSGGNIDMPKFAAILRGETPRD"
     gene            complement(785..1888)
                     /locus_tag="AC16A0_10595"
     CDS             complement(785..1888)
                     /locus_tag="AC16A0_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3105829.1"
                     /translation="MNEARAAALAGLPALGLEILREIFRQGPFLAFGLAMLLLSEGLR
                     VSLGDTSSVPVTVRVAGFLYFLLLTLIGVFPMQFFRVAVFENARSPTRALLKSVRGFF
                     MDDSRLLRGFITLTAIFIFISSFGALKASITLVQPFAWDSAFDDLDRWLHFGYRPWEL
                     LAPLLNHPPVTAVIAVNYHMWFLGLTLYWLYFAFEERPGAFRTQALLAFMLTWSVGGV
                     GLAMLFSSAGPCYFGKLGLGHDPYAELMAYLHETSKSWPVWAVALQDSLWANYANGSG
                     DITKGISAMPSMHNAQCLLLVLAVWNKAPIVRNLAILHGVLVFLGSVQLGWHYAADAY
                     LAWIVAGLAWVASGAFARRLEGAQSFNPAASRP"
     gene            2116..2367
                     /locus_tag="AC16A0_10600"
     CDS             2116..2367
                     /locus_tag="AC16A0_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105830.1"
                     /translation="MPASHMPMIERVARSLAEALGSDDWRSLAPAARAAVAAMREPTP
                     EMLEAALPDLPDWGYLPDDWRAMIDHAVAEPVFPTLHRG"
     gene            complement(2431..2565)
                     /locus_tag="AC16A0_10605"
     CDS             complement(2431..2565)
                     /locus_tag="AC16A0_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105831.1"
                     /translation="MTKTPTETKDSAKQSRKLSPELNEFLHMLQALEGRLDTPATNAR
                     "
     gene            2938..3336
                     /locus_tag="AC16A0_10610"
     CDS             2938..3336
                     /locus_tag="AC16A0_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR3105832.1"
                     /translation="MSASADATTSIQARQACMKANGGVMAVAVPIMKGEKAFDKAALD
                     AAFAKEKEACAGWADWWGDDTKPGGATETWAKEEIWTDKAGFEAAGGAYVKAATAVAA
                     AADEASFKAAFPALGASCQGCHEKFRRPKG"
     gene            3347..>4116
                     /locus_tag="AC16A0_10615"
     CDS             3347..>4116
                     /locus_tag="AC16A0_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3105833.1"
                     /translation="MLSKRNITVLALLAALGLAVLWLLSAPRGLMLADLPQHQPDAAN
                     GEILYHAAGCWSCHKPGTDLKDVDPNLPAGGFPLKTPVGTFYPPNLTPDPETGLGTWS
                     DLDFVNAVQRGVSPAGTNLIPAFPYTSYAHMRVEDVLDIKAYLATLPAVDSPTRSAEI
                     PFPPLLRRGVGLWKWIGLDTTPWAPDASQSASRNRGSYLVNGPGHCAECHTPRNVFMA
                     LDTSQALAGGPHPEGEGRVPSLRGLIERGRYKDAKDIAS"
BASE COUNT          797 a         1301 c         1320 g          698 t
ORIGIN      
        1 cgcggcggca tcgtgcacct gaagcgccgc cagcagcgcg ggcagaccgc gggtgtcatt
       61 accgccacgc gcggcaatca cggccagtca atccccttcg gcgcggcccg ggtcggcatc
      121 ccggtaacgg tggtcgtgcc acacggcaac tcggtggaga agaacgcggc gatgcgagcg
      181 ctcggtgccg agctcatcga agttggccac gacttcgatg cggcgcgcga ggccgccgcc
      241 cgtatcgctg ccgaacgagg tctcgacttc gtcccttcct tcgggccgga cctcgtcgtc
      301 ggcgtttcca cctatgcgca tgaactgttc cgcgccgccg gcgccctcga tgcggtctat
      361 gtgcccatcg gaatgggatc cggcatctgc ggggtcattg ccgcgcgcga catgctgggc
      421 ctgaggacga aggtgatcgg cgtcgccgct gctggcgcga atgcctatgc actgtcattc
      481 gcaacgggaa agccggtgtc gaccaattcc gccgcgacct tcgccgatgg catggcggtg
      541 cgcattccaa acgccgatgc ctttcacgtc atcaagcatg gtgccgaacg gatcgtgaca
      601 gtcagcgacg acgagattgc cgaagccgtg cggctctact tcagggcagc ccatacaatc
      661 gcggaaggag caggtgcagc ggcgctggcc gcgctgatgc aggagcgcga ccgcatgaag
      721 ggcaagcgtg ccggcgtgat cctatccggc ggcaacatcg acatgccgaa gtttgccgcc
      781 attctcaggg gcgagacgcc gcgggattga atgactgcgc gccttcaagg cggcgcgcga
      841 atgcccctga cgcgacccac gccaatcctg cgacgatcca cgcgagatag gcatcggcgg
      901 catagtgcca gccaagctgc acggaaccga gaaacaccag gacgccgtga aggatggcca
      961 ggttgcgcac gatcggcgcc ttgttccaca ccgcaaggac aaggagcagg cattgtgcat
     1021 tgtgcatgct gggcatggcc gagatgccct tggtgatgtc gcccgagccg ttggcatagt
     1081 ttgcccacag tgaatcctgg agcgcgacgg cccagacggg ccaggacttc gacgtctcgt
     1141 gaagataggc catgagctcc gcatagggat catggcccag gccaagcttc ccgaagtagc
     1201 agggtccggc cgaggagaac agcatggcca gccctacgcc gccgaccgac catgtaagca
     1261 tgaaagccag aagtgcctgt gtccggaacg cgccgggtct ctcttcgaag gcgaagtaga
     1321 gccagtagag tgtcagcccg agaaaccaca tgtgatagtt cacggcgatc acggcggtga
     1381 ccggcggatg gttcaaaagc ggtgccagca actcccaggg ccggtagccg aaatgcagcc
     1441 agcggtccaa gtcgtcgaag gcggaatccc aggcgaaggg ttgcaccagc gtgatgctgg
     1501 ccttcagcgc tccgaacgag gaaatgaaga tgaagatcgc cgtcagggtg atgaagccgc
     1561 ggagaagcct gctgtcatcc atgaagaagc cacggaccga cttgagcagc gcgcgcgtgg
     1621 gactgcgcgc attctcaaaa accgccacgc ggaagaactg catcggaaac acgccgatga
     1681 gcgtgagaag gagaaaatag aggaacccgg cgacccggac cgtgacaggc acggaggagg
     1741 tatccccaag agagactctg agaccttcgc tgagcagcag catggccagt ccaaaggcca
     1801 ggaacggtcc ctgtcggaag atctcgcgaa ggatttccag gcccagagcc ggcaatccgg
     1861 caagagccgc ggcccgggcc tcgttcatct cattcacctt tcgccgaaat gaggcggaaa
     1921 gcttaggatc gcccgcttaa ccgaacgtaa agacaggcgc agtggcgcga tgttttgctg
     1981 gggaattgtc gtccgtgaga gttgccttgc ggcggcccag cctctagtga gggtccgggg
     2041 ttgttcaagc acgtgtgtat gcgttcgagg agtgtccggg ccacaggccc ggtttcgcga
     2101 tcggagttgc cccctatgcc tgcttcccac atgccaatga tcgaacgggt cgcccgcagc
     2161 ctggccgaag cgctcggcag cgacgattgg cgaagccttg cgcccgcggc ccgggccgcc
     2221 gtggcggcca tgcgcgaacc gaccccggaa atgctggaag cagcccttcc cgatcttccc
     2281 gattggggtt atctgccgga cgactggcgc gcgatgatcg accacgccgt cgctgaaccg
     2341 gtattcccga ccctgcatcg cggctgaagg ccgcccgaaa aacagaaggg gcgggaaatc
     2401 ccgccccgtt ttccgtcatg caaccggtct ttagcgcgcg ttggttgccg gcgtgtccag
     2461 acgtccctca agtgcctgca gcatgtgcag gaactcgttg agctcagggc tgagcttgcg
     2521 gctttgcttg gcgctgtcct tggtctcggt aggagtcttg gtcatctcat tcaccgtgcg
     2581 tgatcgatcc gagtagtaga ccgtggaact taaggacacc ctaagctctg gcggctgaac
     2641 ctgatccaaa cgccgagttc atccgccgtt caccgatcag gatcactcgt ttcaccggtc
     2701 cttgcggcaa gctcgggccg caggcaaggc aagttccggg catggttaag gcaaggcaaa
     2761 cgcaaccagt cccggcaaca cgaaaatgtg atgtttcagg ccgccggtcc cgggtgtagc
     2821 gtttccacgg ggtccattcg gccccgccaa tcaaaacacc ggaggatcct atcgatgctt
     2881 ggtttccgtg ccctcaaaac tgtaacagcc gccgctctgc ttttgggagc gggcgtcgtg
     2941 tccgcatcgg ccgatgcgac gacttcaatc caggcgcggc aggcctgcat gaaggccaat
     3001 ggtggcgtga tggcagtggc tgtgccgatc atgaagggcg agaaggcctt cgacaaggcc
     3061 gcgctcgacg cggcattcgc gaaggagaag gaagcctgtg ccggctgggc cgactggtgg
     3121 ggcgatgaca ccaagcccgg cggcgcgacc gagacctggg ccaaggaaga gatctggacc
     3181 gacaaggctg gcttcgaggc agccggtgga gcctatgtga aggccgcaac ggctgttgca
     3241 gctgccgccg acgaagcgtc cttcaaggcg gccttcccgg cgcttggggc ctcgtgccag
     3301 ggatgccacg aaaagttccg ccgcccgaag ggctgatcgc ctgacattgc tgtccaaacg
     3361 caacatcacg gtcctggcgc tgctggcagc gctgggactg gccgttctct ggctgctgag
     3421 cgcgccgcgc gggctgatgc tggcagacct gccgcagcat cagccagatg ccgccaacgg
     3481 cgagatcctc tatcacgctg ccggttgctg gtcctgccac aagcccggca ccgatctcaa
     3541 ggacgtcgat ccgaaccttc cggccggcgg atttccgctc aagacacccg tgggcacctt
     3601 ctatccgccc aacctgaccc cggatcccga gaccgggctg ggcacttggt ccgatctcga
     3661 cttcgtcaac gcggtgcagc gcggcgtatc gcctgcaggt accaacctca tccccgcctt
     3721 tccctacacg tcctatgccc atatgcgggt cgaagacgtg ctcgatatca aggcctatct
     3781 ggcaaccttg ccggccgtcg attccccaac gcggtctgcg gaaatcccct ttccacccct
     3841 gctccgccgt ggcgttggcc tgtggaaatg gatcggcctc gataccacgc cgtgggcgcc
     3901 ggatgcatcg cagtcggcaa gccggaatcg cggtagttac ctggtcaatg gtccgggtca
     3961 ttgcgccgaa tgccacaccc cgcgcaacgt cttcatggcg ctcgatacgt cgcaagccct
     4021 ggccggcgga ccgcatccgg aaggggaagg ccgggtcccg tcgctccgcg gcctgatcga
     4081 acgcggccgc tacaaggacg cgaaggatat tgcctc
//
LOCUS       DBNGVR010000508         2670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3136559, whole genome shotgun sequence.
ACCESSION   DBNGVR010000508 DBNGVR010000000
VERSION     DBNGVR010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3136559"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /gene="glgX"
                     /locus_tag="AC16A0_10620"
     CDS             complement(<1..1555)
                     /gene="glgX"
                     /locus_tag="AC16A0_10620"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR3105834.1"
                     /translation="MTCIGDGVNVAVVSRHATCIFICIFDETGKLELGRFALPFRLGD
                     VHYGFVHGIVQGTRYGLRADGPWLPEEGHRFDVSKLLTDPYATRVDGAFTHHADLTRR
                     GAETAQLVPKCIVEAQSDLATVLPPSRPGFIYELPVKAFTMRHPAVPPALRGTVAALA
                     EPCVIEHLLTLGVDTVELMPLAAWIDERHLAQHGLGNAWCYNPVTFMAPDPRLAPGGL
                     AEVRHAVTALHDAGLRVILDVVFNHTGESDLHGATLSLRGLDNALYYRYAGRELVNDT
                     GCGNTVALDRTASVQLAMDAMRSWVMRTGIDGFRFDLAPVMGRMASGFDVDAPLLAAI
                     EQDPLLKDRIMIAEPWDVGPGGYQLGRFPPRWQEWNDRYRDDVRRFWRGDTGAASTLA
                     TRLAGSSDIFGPHRRPWASINYVASHDGFTLRDAVTYTQKRNHANGEDNRDGNPHEPV
                     WPKGDVRALLATLFLSRGTPMLTAGDEFGRSQQGNNNAYAQDNDVTWLDWSTADHALV
                     RFTSELVKLR"
     gene            complement(1600..>2670)
                     /locus_tag="AC16A0_10625"
     CDS             complement(1600..>2670)
                     /locus_tag="AC16A0_10625"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HIR3105835.1"
                     /translation="DYAELMQGLFDFDAIRALIKSGFTMKFDAMGAITGPYGTTILEG
                     LLGFPMGTVMNGIPLPDFGGHHPDPNLVHAKELCDLVLSPTGPDFGAASDGDGDRNLI
                     IGRGMYVTPSDSLAILAANATLVPGYKDGLSGIARSMPTSAAADRVAEKLRIACYETP
                     TGWKFFGNLLDANLATLCGEESFGTGSNHVREKDGLWAVLMWLNILARRRESVADIVR
                     GHWRAFGRNYYSRHDYEEVDADAANGLMAALREKLPRLKGNHGIEAADDFAYDDPVDG
                     SRTTGQGIRLLFASGSRIVYRLSGTGTAGATLRVYIERYEADPGRHDIETQAALSDLI
                     ALSRDIAEIQARTGRSAPTVIT"
BASE COUNT          495 a          834 c          879 g          462 t
ORIGIN      
        1 tgcgcaattt caccagttcc gacgtgaagc ggaccagcgc atgatccgcg gtactccagt
       61 ccagccaagt cacgtcattg tcctgggcat aggcattgtt gttgccctgc tggctgcggc
      121 cgaactcatc gccagccgta agcatgggcg tgccgcgcga caggaaaagc gtcgcgagca
      181 aagcacggac atcgcccttc ggccagaccg gttcatgcgg attgccgtcg cgattgtctt
      241 cgccgttggc gtggttgcgc ttctgcgtgt aggtgacagc atcgcgcagg gtaaagccgt
      301 catgcgatgc aacatagttg atgctggccc atggtcttcg gtgcggcccg aagatgtcgg
      361 aagacccggc cagccgtgtg gccagtgtac tggcggcacc cgtgtcgccg cgccagaagc
      421 gccgcacgtc gtcgcggtag cggtcgttcc attcctgcca gcgcggcgga aaccgcccga
      481 gctggtagcc gcccggcccg acatcccagg gctcggcaat catgatgcgg tccttcagaa
      541 ggggatcctg ctcgatggcc gcaagcagcg gcgcgtcaac gtcgaagccc gaagccatac
      601 gccccatcac cggcgcaagg tcgaagcgga agccgtcgat gccggtgcgc ataacccagc
      661 tgcgcatcgc atccatcgcc agttgcaccg acgcagtccg gtcaagtgca accgtgttgc
      721 cgcaaccggt gtcattcacc agttcacggc ctgcatagcg gtagtagagc gcattgtcga
      781 ggccgcgcag cgacagcgtg gcgccatgca ggtcgctctc gcccgtatgg ttgaacacaa
      841 catcgaggat gacgcgaagg ccggcatcat gaagcgccgt gacggcgtgg cggacctcgg
      901 caaggccgcc gggcgccagc cgcggatcgg gcgccatgaa ggtgaccgga ttgtagcacc
      961 aggcattgcc aagcccgtgt tgcgccagat gacgctcgtc gatccaggcc gccagcggca
     1021 tgagttcgac ggtgtcgacg cccagcgtca agagatgttc gatcacgcag ggttcggcga
     1081 gtgctgcaac cgttccgcgc agcgcaggcg gcacggccgg atgccgcatg gtgaaggcct
     1141 tgaccggcaa ctcatagatg aagcccggcc gcgacggcgg caggacagtt gcgagatccg
     1201 actgcgcctc gacgatgcac ttcggaacga gttgcgcggt ttctgcgcca cgccgcgtga
     1261 gatcggcatg atgcgtgaag gcgccgtcga ctcgcgtggc ataaggatcg gtcagcagct
     1321 tcgacacatc gaagcggtgc ccctcttccg gcagccacgg gccatcggcg cgcaagccat
     1381 agcgggtgcc ctgcacgatg ccgtgaacga aaccgtaatg cacatcgccg aggcggaacg
     1441 gcagggcgaa gcgtcccaat tcgagtttgc ccgtctcgtc gaagatgcaa atgaagatgc
     1501 aggtggcgtg ccgcgatacg acggcaacat tgacgccatc tccgatgcag gtcacgccaa
     1561 gcggtgaagg ttgcccttcc gtggttgaga ggcgcagcgt caggtgatca ccgttggcgc
     1621 gctgcggccg gtgcgtgcct ggatctccgc aatgtcgcgc gacagcgcga tgagatcaga
     1681 aagagccgcc tgcgtttcga tgtcgtggcg accggggtca gcctcgtagc gttcgatata
     1741 gacgcgcagc gttgcgcctg ccgtgcccgt accggaaagg cggtagacga tgcgcgatcc
     1801 gctggcgaag agcaggcgga tgccctggcc ggtggtccgc gacccatcga ccggatcgtc
     1861 ataggcaaaa tcatccgccg cttcgatgcc gtgattgccc ttgagcctgg gcagcttctc
     1921 gcgcagcgcc gccatcagtc cgttcgctgc gtcggcgtcc acttcctcgt agtcgtggcg
     1981 cgaataatag ttgcggccaa aggcccgcca atgtcctcgc acaatgtccg ccacgctctc
     2041 gcgccgccgc gccaggatgt tgagccacat cagcacggcc cagagcccgt ccttctcgcg
     2101 cacatggttc gagcccgtgc cgaagctttc ctcgccgcag agcgtggcca ggttcgcgtc
     2161 cagaagattg ccgaagaatt tccagccggt cggcgtctcg tagcaggcga tgcgaagctt
     2221 ctctgccacg cgatcggcag cggcactggt cggcatggag cgggcgattc cggaaagccc
     2281 atctttgtaa cctggcacca gcgtggcatt ggcggcgagg atggcgaggg aatccgacgg
     2341 cgtcacatac atgccgcggc cgatgatcag gttgcggtcg ccgtcaccgt ccgaggccgc
     2401 gccgaagtcc ggccccgttg gcgaaagcac gaggtcgcag agttcctttg catgaacgag
     2461 gttgggatcg ggatggtggc cgccgaagtc cggcagcggg atgccattca tcaccgtccc
     2521 catggggaag ccgagcaggc cttcgagtat ggttgtacca taggggccgg tgatggcacc
     2581 catggcgtcg aacttcatgg tgaagccgga tttgatcagt gcacggatgg catcgaaatc
     2641 gaacaggccc tgcatcagtt cggcatagtc
//
LOCUS       DBNGVR010000509         7293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_1371004, whole genome shotgun sequence.
ACCESSION   DBNGVR010000509 DBNGVR010000000
VERSION     DBNGVR010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_1371004"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /gene="araD1"
                     /locus_tag="AC16A0_10630"
     CDS             complement(<1..697)
                     /gene="araD1"
                     /locus_tag="AC16A0_10630"
                     /inference="COORDINATES: protein motif:HMM:NF040903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HIR3105836.1"
                     /translation="MNLVQIRDSKGKRRVGLVDGGSIVLLKKVATTLDLARLALKNGQ
                     KLAAAATALAGSETEDYARALRQKRVLTPVDHPDPAHCIITGTGLTHLGSAAARDGMH
                     KKLSGATEELTDSLKMFKMGLEGGKPKSKSEAGVQPEWFYKGDGSWLVGPGQPLPMPS
                     FALDGGEEPELAGLYYIDEKGRPVRLGFALGNEYSDHVTERQNYLYLAHSKLRHSAIG
                     PEMVLGKIPESIEG"
     gene            complement(749..1660)
                     /locus_tag="AC16A0_10635"
     CDS             complement(749..1660)
                     /locus_tag="AC16A0_10635"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3105837.1"
                     /translation="MTITEIPVTCLVDCKDKLGEGCFWDAATQCLWWLDLIVPSTIHR
                     LHVASGAHRSWQFSEMVTAMARRRDGTILVGSHRGLNAFDPATGALSPRTRIGTDLPG
                     NRGNDGACDAMGRFWFGTMMNNVGDLGADLPITADTGLLYRVDADGTATIMERNIGVS
                     NGPCWSPDNRIFYFTDSRNQVIWAYDFDLAAGTISNRRVFNDSRDHGYPDGATVDAEG
                     FLWSARWEGACVLRIDPKGRIDRIVPMPARRVTNVCFGGAKLDTLYVTTARLHVPEED
                     LRRHPLQGGLFCFDPGVTGFEKHAFAG"
     gene            complement(1657..2694)
                     /locus_tag="AC16A0_10640"
     CDS             complement(1657..2694)
                     /locus_tag="AC16A0_10640"
                     /EC_number="4.2.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0050346 - trans-L-3-hydroxyproline
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-3-hydroxy-L-proline dehydratase"
                     /protein_id="HIR3105838.1"
                     /translation="MQSARAIQIVGCHAEGEVGDVIVGGVAPPPGTTVWEQSRFIARD
                     GGLRAFVLNEPRGGVFRHVNLLVPPRDPRAAMGFIIMEPEDTPPMSGSNCICVATVLL
                     DTGILPMTEPETRLVLEAPAGLVEIVAQCRNGKAQSITLRNVPAFADRIAVPLEVEGL
                     GTILVDTAFGGDSFVMASARALGFDIRPDEARDLAVTGRKIVKSANAQLGFVHPDLPD
                     WTHFSFAFLTGELERVDGCLSSRNACVVKPGKIDRSPTGTGCSALMAVLHAKGLMQVG
                     DRYIGRSIIESRFIGEIADRTTVGNHAAIIPTITGRAWITGQSTLMLDPSDPWPDGYK
                     ISDTWPQDAKT"
     gene            2858..3868
                     /locus_tag="AC16A0_10645"
     CDS             2858..3868
                     /locus_tag="AC16A0_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3105839.1"
                     /translation="MEFIQTALFSDFLGTPAFFWMAFITIVVGLLVFDLGFLHKGQKE
                     IEAKESFILYAGYVAIAFAFGAWVWWARGAQSGLEFFTGYLIEQSLAMDNIFVIATVF
                     TFLGIPRIYQHRVLFWGILGVIVFRAILIGLGAALVMSFSWILFVFGAFLVFTGLKMF
                     KTSSEKPSVEDNLILRLIKKNFRVTDTLHGEKFTVKLPDPKTGKVVTFITPLLVALIM
                     VEFVDLIFAVDSVPAIFAVTQDPFIVYTSNIFAILGLRALYFALAAAIHRFKYLQYSL
                     AVILVLVGIKIFLVPLGIKIDTALSLFVTVSVLASGIFYSLWKTRNDKPEPLPEPLKA
                     ES"
     gene            3928..4443
                     /locus_tag="AC16A0_10650"
     CDS             3928..4443
                     /locus_tag="AC16A0_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HIR3105840.1"
                     /translation="MKKLEHLIESIILASRWLLVVFYLGLGLALALYAVSFAFKVWDF
                     AEKVAYLGETDTILKMLSLIDAALVASLLVMVIISGYENFVSRFDNAEGELSWLGKID
                     AGSLKIKVASTIVAISSIHLLQVFLNAQQYSDDKLMWFTIIHITFVISALFLAYIDKV
                     MADTKKSKGGD"
     gene            complement(4450..5880)
                     /locus_tag="AC16A0_10655"
     CDS             complement(4450..5880)
                     /locus_tag="AC16A0_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR3105841.1"
                     /translation="MMTGPTFLPALRSLAAILALASLAGPAMAAEGPDGRSLGLAWSL
                     PFVGMLLSIALGPLLVPHFWEDHRGKISAFWALLAIIPMAIFAGPGTALHAVAHTALL
                     EYIPFILLLLALFTVAGGIVIRGNLHGTPLTNTMLLAIGTLMASVIGTTGAAMIMIRP
                     VIRANDHRKSNAHVVVFFIFLVANAGGALTPLGDPPLFLGFLRGVDFFWPTFHLFLPT
                     AFLVAALLGIFFLFDSWIGARAGELRPDPTPDRDLSVAGGINFFLIPAIIAAILFSSS
                     IDLGGFSLFGTPIAFANLARDLAFIVIAMLSIGYSPRGSRAENNFSWEPIKEVAELFA
                     GIFITIIPVLAMLRAGSEGAFALLVDLVARPDGTADNLAYFWSTGGLSSFLDNAPTYI
                     VFFELAGGDPQFLMTQGAATLAAISLGAVFMGANTYIGNAPNFMVYAIARDAGVRMPS
                     FFGYMLWSCTILLPLFVLLSLLFFRS"
     gene            complement(5930..6706)
                     /locus_tag="AC16A0_10660"
     CDS             complement(5930..6706)
                     /locus_tag="AC16A0_10660"
                     /inference="COORDINATES: protein motif:HMM:NF025389.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR3105842.1"
                     /translation="MLPDVIFQWSLGSSFLFVLAVTGAVAVALHLIFFLPPLQRLSIR
                     IGDLSPILQNLAGTFFALSVAFLASAVWATEDRAHEIVNDEALSIRRVSTYAQALSAP
                     SRDTVQNLIRDYAKAVEREWPSMSDSESDKPANRDISALYAAIISGLSENEQNRMLQQ
                     RLLTAVDAMVQAREHRLTAARETVSDGQWIMVSLLAILLLVVIAAFHGRWPASRAAAL
                     VLITFAISIPLFVILAHDRPFAGYQAISPEPILAAGGLLR"
     gene            complement(6719..>7293)
                     /locus_tag="AC16A0_10665"
     CDS             complement(6719..>7293)
                     /locus_tag="AC16A0_10665"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3105843.1"
                     /translation="VHVSLGDDQGHNLFAAEDPQKNALLMQAIGGLKAAMAESMLIFA
                     PNANSYRRFRRNSYAPVSASWGINNRTVSLRIPAGAANACHIEHRPSGADANPYLVMA
                     AILAGMHHGITEKADPGNPVTGNGYEKRAKYIPGNWFDAIDAFWRASILKEYFGKDFV
                     DTYCTLKEVEADRFYAEPTTRDFEWYLRTV"
BASE COUNT         1342 a         2224 c         2383 g         1344 t
ORIGIN      
        1 tgccctcgat cgactcgggg atcttgccca gcaccatttc cggcccgatg gcggaatggc
       61 gcagcttgga atgggcgaga tagagatagt tctgccgctc ggtgacatgg tcggagtact
      121 cgttgcccag cgcgaaaccg aggcggaccg ggcggccctt ctcgtcgata tagtagaggc
      181 ccgccagttc cggctcctcg ccgccgtcaa gtgcgaagga cggcataggc agcggctggc
      241 cgggtccgac cagccatgac ccgtcgccct tgtagaacca ctcgggctgc acgcctgctt
      301 cggacttgct tttcggtttg ccgccttcga gtcccatctt gaacatcttg agactgtcgg
      361 tcagttcctc ggtggcgccc gacagtttct tgtgcatgcc gtcgcgcgcg gcggccgagc
      421 ccagatgagt gaggccagtg ccggtgatga tgcaatgggc cgggtcggga tgatcgaccg
      481 gcgtcagcac ccgcttctgc cggagcgcgc gggcatagtc ctcggtctcg ctgccggcca
      541 gcgccgtggc cgctgcggcg agcttctggc cgtttttcag cgccaggcgc gcgaggtcga
      601 gcgtggtggc cactttcttc agcagcacga tggaaccgcc atcgacgagg ccgacacgcc
      661 gtttgccctt gctgtcccgg atctgcacga ggttcatcgc tgccactccc atttgtgcgt
      721 tttattgtat gacaattagc gggtttgcct agccggcgaa agcatgtttt tcgaaaccgg
      781 taacaccggg gtcgaagcag aagagcccgc cctgcaaggg atgccggcgc aggtcctcct
      841 ccggcacgtg cagccgcgcg gtggtgacgt agagcgtgtc gagcttcgcg ccgccgaagc
      901 agacgttggt gacgcgccgc gccggcatcg ggacgatgcg gtcgatccgg cccttcggat
      961 cgatgcggag cacgcaggcg ccctcccagc gcgcgctcca caggaagccc tcggcatcga
     1021 cggtggcacc atcgggatag ccgtggtccc tgctgtcatt gaacacgcgg cggttggaga
     1081 tggtccccgc ggcgaggtcg aagtcatagg cccagatcac ctggttacgc gagtcggtga
     1141 agtagaagat gcgattgtcg ggcgaccagc aggggccgtt ggagacgccg atgttgcgtt
     1201 ccatgatcgt ggccgtgccg tcggcgtcga cgcggtagag caggcccgtg tcggcggtga
     1261 tcggcaggtc ggcaccgagg tcgcccacat tgttcatcat tgtgccgaac cagaaccggc
     1321 ccatggcatc gcaggccccg tcattgccgc gattcccggg aaggtccgtg ccgatgcggg
     1381 ttcgggggga cagcgcgccg gttgcgggat cgaaagcgtt caggccgcgg tgcgacccca
     1441 ccaggatggt gccgtccctc cgcctggcca tggcggtgac catttcggag aactgccagc
     1501 tgcgatgtgc gccgctggcc acatgcaggc ggtggatggt ggagggcacg atgagatcga
     1561 gccaccacag gcactgcgtg gcggcgtccc agaaacagcc ctcgccgagc ttgtccttgc
     1621 aatccacgag gcaggtcacg gggatttcgg tgatcgtcat gtctttgcat cctggggcca
     1681 ggtgtcggag atcttgtagc catcgggcca ggggtcggag gggtcgagca tgagagtcga
     1741 ctgcccggtg atccaggccc ggcccgtgat ggtgggaatg atggcggcgt ggttgccgac
     1801 ggtggtgcgg tccgcgatct cgccgatgaa acggctctcg atgatggagc ggccgatgta
     1861 gcggtcgccg acctgcatca ggcccttcgc atggagcacc gccatcagcg ccgagcagcc
     1921 cgttccggtg ggggagcggt cgatcttgcc gggtttcacc acgcaggcat tgcggctcga
     1981 caagcaaccg tcgacccgct cgagctcgcc ggtgaggaat gcaaaggaga aatgcgtcca
     2041 gtccggcaga tcgggatgga caaagccaag ctgggcgttg gcggacttca cgatcttccg
     2101 ccccgtgacg gcgagatcgc gagcctcgtc ggggcggatg tcgaagccca gcgccctggc
     2161 actggccatc acgaaactgt cgccaccgaa agcggtgtcg accaagatcg tgccaaggcc
     2221 ctcgacctcg agcggaaccg cgatccggtc ggcgaaggcc ggcacattgc gcagggtgat
     2281 cgactgcgcc ttgccgttcc ggcattgcgc cacgatttcg accagaccgg ccggcgcctc
     2341 cagcaccagc cgggtctcgg gctcggtcat gggcaggatg ccggtgtcga gcaggaccgt
     2401 cgccacgcag atgcagttgg agcccgacat gggcggggtg tcctcgggct ccatgatgat
     2461 gaagcccatg gcggcgcggg ggtcccttgg cggcaccagc aggttcacat ggcggaagac
     2521 cccgccgcgg ggctcgttca gcacgaaggc acggagccca ccgtcgcggg cgatgaagcg
     2581 cgactgctcc cagacggtgg ttcccggcgg cggggccacg ccccccacga tcacgtcgcc
     2641 cacttcgcct tcggcatggc agccgacgat ctgaatggcc ctggcgcttt gcatcgaatc
     2701 ccctccgccc gggaaatcta gacgattttt ctggcaggcg gggcttgaac ttgccacatt
     2761 tgcgcctaca tcggcccggt ggcgccgggc ccctctggcg aggccttctc gcgatgtcgg
     2821 agccgcaaaa gttcaacaat atcaaaggtg gtccggcatg gaattcatcc aaactgcatt
     2881 gttttcggat tttctcggca cgccggcctt cttctggatg gccttcatca ccatcgtggt
     2941 gggcctgctg gtcttcgacc tgggcttcct ccacaagggc cagaaggaga tcgaggcgaa
     3001 ggagagcttc atcctctatg ccggctatgt ggccattgcc ttcgccttcg gtgcctgggt
     3061 ctggtgggcg cggggtgccc agtcgggcct cgaattcttc accggctacc tgatcgaaca
     3121 gtcgctggcg atggacaaca tctttgtcat cgccacggtc ttcacctttc tcggcattcc
     3181 ccgcatctat cagcaccggg tgctgttctg gggcattctc ggggtgatcg tgttccgcgc
     3241 catcctgatc ggcctgggcg cggcgctggt catgtccttc tcctggatcc tcttcgtctt
     3301 cggcgccttc ctggtcttca ccggcctcaa gatgttcaag acatccagcg aaaagcccag
     3361 cgtcgaggac aatctgatcc tgcggttgat caagaagaac ttcagggtga ccgacacgct
     3421 gcatggcgag aagttcacgg tgaagcttcc ggatcccaag accggcaagg tggtgacctt
     3481 catcaccccg ctgctcgtgg cgctgatcat ggtggagttc gtcgatctca tcttcgccgt
     3541 tgattcggtt cccgccatct tcgcggtcac ccaggatccc ttcatcgtct acacctcgaa
     3601 catctttgcc atcctcggcc tgcgcgcgct gtacttcgcc ctggccgccg ccatccaccg
     3661 cttcaagtac ctgcagtact cgctggcggt gatccttgtg ctggtcggca tcaagatctt
     3721 cctagtgccg ctgggcatca agatcgatac ggcactgtcg ctcttcgtga ccgtgtcggt
     3781 tctggcctct ggcatcttct actcgctgtg gaagacccgg aacgacaagc cggaaccctt
     3841 gcccgagccc ctcaaggccg aaagctgacc cctcttgcct ccctgatagg atgcgctatc
     3901 aggaaggcgt cccagcgggg gtccgcaatg aagaagctcg aacacctcat cgaatccatc
     3961 attctggcct cgcgctggct tctggtggtc ttctatctcg gcctcggcct tgcgcttgcg
     4021 ctctacgccg tgtccttcgc cttcaaagtc tgggacttcg ccgaaaaggt ggcctatctg
     4081 ggcgagaccg atacgatcct gaagatgctg tcgctgatcg atgccgcgct ggtggccagc
     4141 ctgctggtga tggtgatcat ctcgggctac gagaatttcg tcagccgctt cgacaatgcc
     4201 gagggcgagc tgtcgtggct cggcaagatc gacgccgggt cgctcaagat caaggtggcc
     4261 tccaccatcg tggccatttc ctcgatccac ctgctgcagg tcttcctgaa cgcccagcag
     4321 tattccgacg acaagctgat gtggttcacc atcatccaca tcaccttcgt catttcggcg
     4381 ctcttcctgg cctatatcga caaggtcatg gcggatacga agaagtccaa gggcggcgac
     4441 tgacggcgct cagctccgga agaacaggag cgacagcagc acgaagagcg gcagcaatat
     4501 ggtgcatgac cagagcatgt agccgaagaa actcggcatg cggacgccgg cgtcgcgggc
     4561 gatggcatag accatgaagt tcggggcgtt gccgatatag gtgttggcgc ccatgaagac
     4621 ggcacccagc gaaatggcgg caagcgtcgc cgcaccctgg gtcatgagga actgcggatc
     4681 gccgccggcc agttcgaaga acacgatata ggtcggggca ttgtcgagga aggacgagag
     4741 gccgcccgtc gaccagaaat acgcgagatt gtcggcggtg ccgtccggcc tcgcgacgag
     4801 atcaaccagg agcgcaaagg cgccctcgct tcccgcacgc agcatggcaa gcaccggaat
     4861 gatggtgatg aagatgccgg cgaagagctc ggccacttcc ttgatcggct cccagctgaa
     4921 gttgttctcc gcccggctgc cacgcggcga atagccgatc gacagcatgg cgatgacgat
     4981 gaacgccaaa tcccgcgcca gattggcgaa agcaatgggc gtgccgaaca ggctgaaacc
     5041 gccaagatcg atgctgcttg agaagagaat ggcagcaatg attgccggga tcagaaagaa
     5101 gttgattccg cccgccacgg agagatcgcg gtcgggggtc gggtcaggcc gcagctcgcc
     5161 ggcacgcgcg ccgatccagc tgtcgaacag gaagaaaatg cccagcaatg cggcaaccag
     5221 aaaggcggtt ggcagaaaca ggtggaaggt gggccagaag aagtcgaccc cgcgcaggaa
     5281 accgagaaag agcggcgggt cgcccagcgg tgtcagggcg ccaccggcat tggcgaccag
     5341 gaagatgaag aagaccacga catgcgcatt gctcttgcgg tgatcgttgg cgcggatcac
     5401 cggccggatc atgatcatgg ccgcgccggt cgttccgatg acgctggcca tcagcgtgcc
     5461 gatggccagc agcatcgtgt tggtgagcgg ggtaccgtgc aggttccctc ggatgacgat
     5521 gccaccggcc acggtaaaga gggcgagcag caggaggatg aaagggatgt actcgagcag
     5581 agccgtatga gcgacggcat gcagggcggt tccggggccc gcgaaaatcg ccatcgggat
     5641 gatcgccagc agggcccaga aggccgagat cttgccccga tggtcttccc agaagtgcgg
     5701 aacaagcaat gggccgaggg cgatcgacag cagcatgccg acgaagggca gcgaccatgc
     5761 gaggcccagg ctgcggccat cgggcccctc agccgccatt gccggcccag cgagcgaggc
     5821 tagcgcgagg attgccgcaa ggctgcggag ggcggggaga aaggtcggtc ctgtcatcat
     5881 gttcggagcc acttgtgccg tcttgaccct gcgactgcaa gttagacgat cagcggagaa
     5941 gaccgcctgc tgccaggatc ggctcggggc tgatcgcctg gtaacccgcg aagggccgat
     6001 catgggccag gatcacgaag agcggaatgg aaatggcgaa ggtgatcagc accagtgcgg
     6061 cggcccgcga ggccggccag cgcccgtgaa aagctgcaat cacgacgagg aggagtatcg
     6121 ccaggaggct caccatgatc cattggccgt cggaaacggt ttccctggct gcggtcaggc
     6181 ggtgctcgcg ggcctgcacc attgcatcca ccgcagtgag caggcgctgc tgcagcatgc
     6241 ggttctgctc gttctccgaa agcccggaga tgatcgcagc gtaaagcgct gagatgtcgc
     6301 ggttggccgg cttgtcgctc tcgctgtccg acatgctggg ccactcccgc tcaaccgcct
     6361 ttgcgtagtc gcggatgagg ttttggaccg tatcgcgaga tggtgcgctc agcgcctgcg
     6421 cgtaggtgct cacccgcctg atgctcaggg cttcgtcatt cacgatttca tgggcgcggt
     6481 cctcggtggc ccatacagcg ctggcaagaa aggccaccga caacgcgaag aaggtgcccg
     6541 cgagattctg caggatcggc gacaggtcac cgatccggat gctcagccgt tgaagtggcg
     6601 gcaggaaaaa gatcagatgc agcgccacgg ccacggcccc ggtcacagcc agcacgaaga
     6661 ggaaactgct gcccagagac cattggaaaa tgacgtccgg cagcatgttt ccgttctcct
     6721 agaccgtccg cagataccac tcgaagtcgc gcgtcgtggg ttccgcatag aagcgatcgg
     6781 cctcgacttc cttcaaggta cagtaagtat cgacaaagtc cttgccgaaa tactccttga
     6841 ggatcgaggc ccgccagaac gcgtcgatgg cgtcgaacca gttgcctgga atatacttgg
     6901 cccgcttttc gtaaccgttg cccgtcacgg gattgccggg atctgccttc tcggtgatgc
     6961 cgtgatgcat gccggcgagg atggctgcca tcacaagata tggattggcg tctgccccgc
     7021 tgggccgatg ctcgatgtgg caggcattgg cggcgccggc cggaatgcgc aggctcaccg
     7081 tgcggttgtt gatgccccag ctggccgaga ccggcgcgta ggaattgcgg cggaagcggc
     7141 ggtaggaatt ggcattgggc gcgaagatca gcatggattc cgccatggcc gccttaagcc
     7201 cgccgatggc ctgcatcagc agcgcgttct tctgcgggtc ttcggcggcg aagagattgt
     7261 ggccctgatc gtcgccgagg cttacatgca cat
//
LOCUS       DBNGVR010000510         3223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4120434, whole genome shotgun sequence.
ACCESSION   DBNGVR010000510 DBNGVR010000000
VERSION     DBNGVR010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4120434"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="AC16A0_10670"
     CDS             complement(<1..1260)
                     /locus_tag="AC16A0_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR3105844.1"
                     /translation="MAHDAGDGSHLAIDPVCGMSVDPHATQLTHAHGGKTYYFCAPGC
                     LTKFAADPGRYLDPESMKKALEALPQDAVFTCPMHPEIEQIGPGTCPICGMALEPKFA
                     GAETGPNHELIDMTRRFWIGLVLTLPVFALEMGSHVFGLHGLIPRATSNLIQFLLATP
                     VVLWAGWPFFVRGWQSLVTRNLNMFTLIAIGTGVAYAYSIVATFMPQLFPQAFRDHDG
                     TVAVYFEAAAVITVLVLLGQVLELRARDKTSGAIKALLGLTPRMARRVEADGSEGDVE
                     IDAVEAGNILRVRPGEKVPVDGLVTEGHVSIDESMVTGESLPVSKQPGSKVIGGTLNA
                     SGSFLMRAEKVGRETMLARIVQMVAEAQRSRAPIQRLADQVSSWFVPLVMLASLLAFA
                     AWAIWGPEPRFAFGLIAAVTVLIIACPC"
     gene            1432..2028
                     /locus_tag="AC16A0_10675"
     CDS             1432..2028
                     /locus_tag="AC16A0_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HIR3105845.1"
                     /translation="MAQSVQLPARRGLARPMNRGQSVRVVNTHGQQVVDTWAFSRDQI
                     REFMSMEHSRVHMGRVQPITGSTLLSNRRNPMLTIVEDTSGGVHDTLMAACDGYRYRM
                     LGCTGHHDNCTENLHAALASLNLQAPETPAPLNLFQNSAVGSDGSLVIAPPVAPPGSH
                     VTLRAEMDLVIVFSACPQDMMPTNGADMTPKDVELVFG"
     gene            complement(2032..2466)
                     /gene="derI"
                     /locus_tag="AC16A0_10680"
                     /gene_synonym="eryI"
     CDS             complement(2032..2466)
                     /gene="derI"
                     /locus_tag="AC16A0_10680"
                     /gene_synonym="eryI"
                     /EC_number="5.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythrulose-4-phosphate isomerase"
                     /protein_id="HIR3105846.1"
                     /translation="MKIALGADSAGKPLLDVIEAHLKTKPEHQISNLSDAGFYADMAA
                     GVAKKVKDGSFDRAILVCGTGIGVCISANKVPGIRAALTHDTYSAERAAKSNNAQVIT
                     MGARVIGPELAKAIVDTWLVSEFDPKGSSAANVEAIDRLDQR"
     gene            complement(2463..>3223)
                     /locus_tag="AC16A0_10685"
     CDS             complement(2463..>3223)
                     /locus_tag="AC16A0_10685"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3105847.1"
                     /translation="WIGTSWKMNKTRREARAFAEALKASPYASTAQAQPFVIPPFPYI
                     AEVADILSGTRVKIGAQNMHWADHGAWTGEISPLMVRDCGAHLVELGHSERRTHFGET
                     DETVARKVAAALRNGLIALVCIGDTKEEHAAGATADVLARQTRFALRDVAAAAAGKVI
                     LAYEPVWSIGEGGIPADPAFANDQHKRIKALTVEVCGRALPVLYGGSVNPQNCCELAR
                     QSDIDGLFIGRSGWNAEGYIGIIEAVTAALGEHA"
BASE COUNT          565 a         1045 c         1043 g          570 t
ORIGIN      
        1 gcagggacag gcgatgatca gcaccgtcac cgcggcaatg agtccgaagg caaatcgcgg
       61 ttccggaccc cagatcgccc aggccgcgaa ggccagcagg ctggccagca tgacgagcgg
      121 aacgaaccag gaggacacct gatcggcaag ccgctggatc ggcgcacggc tgcgctgcgc
      181 ctcggctacc atctgcacga tgcgggccag catggtctcg cgccccacct tctcggcgcg
      241 catgaggaag gaccccgagg cattgagcgt gccgccgatg accttggatc ccggctgctt
      301 gctgacaggc aaggactctc ccgtcaccat tgattcgtcg atggagacat ggccttccgt
      361 caccagaccg tcgacgggaa ccttctcgcc cggccgtacg cgaaggatgt tgccggcttc
      421 gacggcgtcg atctcgacgt ccccttcgct gccatcggcc tcgacccgcc gcgccatccg
      481 cggcgtcagc ccgagcaggg ccttgatggc gccggaggtc ttgtcgcgtg cgcgcagttc
      541 gaggacctgc cccagcagca ccagcacggt gatgacggcg gcggcctcga aatagaccgc
      601 gacggttccg tcatgatcgc ggaaggcctg cgggaaaagc tgcggcatga aggtcgccac
      661 gatggaatag gcataggcga cgccggttcc gatggcgatc agcgtgaaca tgttgagatt
      721 gcgggtgaca agggactgcc agccgcgcac gaagaagggc cagccggccc acagtaccac
      781 cggcgttgcc agcaggaact ggatgagatt cgaagtggcc cgcggtatca gaccatgcag
      841 gccgaagacg tggctgccca tctcgagcgc gaagaccggt agcgtcagga caagcccgat
      901 ccagaagcgc ctggtcatgt cgatcagctc gtgattgggg ccggtctccg ctcctgcaaa
      961 cttgggctcc agtgccatgc cgcagatcgg acaggttccg ggaccgatct gctcgatctc
     1021 gggatgcata gggcaggtga agaccgcatc ctgcggcagc gcctcgagcg ctttcttcat
     1081 ggactccggg tcgagataac ggccgggatc ggcagcgaac ttggtgaggc agcccggcgc
     1141 acagaagtag taggtcttgc cgccgtgggc atgcgtgagc tgcgtcgcgt gcggatcgac
     1201 cgacatgccg cagacgggat cgatcgccag gtgtgagccg tcaccggcgt catgagccat
     1261 cttcaatcct caggctgttt cattgcgacc gctggcctta tagaccttcc gcggccagac
     1321 aggtcaagcg ccgtgctggc ccgttgcgca aggcgcaatg cagctatggc gacaaccggc
     1381 agtgttgcgt accgcgtggt attggccaat tgttcatgtg gaggtcgctc tatggctcaa
     1441 tctgtccaac tccctgcgcg gcgcggactc gcccgtccca tgaacagggg gcagagcgtc
     1501 cgcgtggtga atacccatgg ccagcaggtg gtcgacacct gggccttctc gcgcgaccag
     1561 atccgcgagt tcatgtccat ggagcattcg cgcgtgcaca tgggccgggt tcagcccatc
     1621 acgggatcga cgctgctcag caaccgccgc aacccgatgc tgaccatcgt cgaggatacg
     1681 tcgggcgggg tccatgacac gctgatggcg gcctgcgacg gctaccgtta ccgcatgctt
     1741 ggctgcaccg ggcaccacga caactgcacc gagaatttgc acgccgcgct tgcaagcctc
     1801 aatctgcagg cgcctgaaac gccagcaccc ctcaacctct tccagaactc cgcggtgggt
     1861 agcgatggtt cgctggtgat cgccccgccc gtcgcgccgc cgggctcgca cgtgacgctg
     1921 cgggccgaga tggacctcgt gatcgtcttc tcggcctgtc cgcaggacat gatgccgacc
     1981 aatggcgccg acatgacgcc gaaggatgtg gaactggtct tcggctgagg atcagcgctg
     2041 atccagccgg tcgatagcct cgacattggc cgccgaactc cctttcggat cgaactcgga
     2101 gacgagccat gtgtcgacga tggccttggc gagttccgga ccgatgacgc gcgcgcccat
     2161 ggtgatgacc tgggcgttgt tggacttcgc cgcgcgttcg gcggaatagg tatcgtgggt
     2221 gagcgcggcg cggatgccgg gcaccttgtt ggccgagatg cacacgccga tgcccgtgcc
     2281 acagaccagg atggcgcgat cgaagctgcc gtccttgacc ttcttcgcaa cgccggccgc
     2341 catgtcggca tagaagccgg catcgctcag attgctgatc tgatgttcgg gcttggtctt
     2401 gagatgcgct tcgatgacat cgagaagcgg cttgccggcg ctgtcggcgc ccagtgcgat
     2461 cttcatgcgt gttctcccag tgcggccgtc accgcctcaa tgattccaat gtagccttcg
     2521 gcgttccagc cggaacgccc gatgaagagc ccgtcaatat ccgattgccg cgccagctcg
     2581 caacaattct gcggattgac gcttccgccg taaagcaccg gcagcgccct gccgcaaacc
     2641 tccaccgtca gcgccttgat gcgcttgtgc tggtcgttgg caaaggcggg atcggccggg
     2701 attccgccct cgccgatgga ccataccggc tcataggcca ggatgacctt ccccgccgcc
     2761 gctgcggcca cgtcgcgcag ggcaaaacgt gtctggcgcg ccagaacatc ggcggtggcg
     2821 cctgctgcgt gctcctcctt ggtgtcgccg atgcagacca gggcgatcaa cccgttccgc
     2881 agcgctgcgg ccaccttgcg cgccaccgtc tcgtcggtct cgccgaaatg ggtgcggcgc
     2941 tcgctgtggc ccagctcgac gagatgggcg ccgcaatcgc gaaccatgag cggcgagatt
     3001 tcgccggtcc aagcgccatg gtcggcccag tgcatgttct gcgcgccgat cttcaccctg
     3061 gtgcctgaaa gaatgtccgc cacctcggcg atatagggga agggcgggat gacgaagggc
     3121 tgcgcctgcg cggtgctggc atatggcgat gccttgaggg cttccgcgaa ggcacgcgcc
     3181 tcgcgcctcg tcttgttcat cttccagctg gtgccgatcc acg
//
LOCUS       DBNGVR010000511         2398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3727790, whole genome shotgun sequence.
ACCESSION   DBNGVR010000511 DBNGVR010000000
VERSION     DBNGVR010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3727790"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC16A0_10690"
     CDS             <1..503
                     /locus_tag="AC16A0_10690"
                     /inference="COORDINATES: protein motif:HMM:NF028177.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HIR3105848.1"
                     /translation="PADFRLPEPTRLPVLKYLPDCIGQAMLLDADLAAALAAIEDDLH
                     WARSASYSDAVLGEGFCDNYGWAELIGSGGFFDGDDFRLGLLMLGPDRHYKDHYHPAP
                     ELYWPLTGPSDWKAGAGGFVTRSAGEIIWHIPWKVHATATGSTPLLAVWSWTRDADTP
                     AKLVGA"
     gene            500..1312
                     /locus_tag="AC16A0_10695"
     CDS             500..1312
                     /locus_tag="AC16A0_10695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3105849.1"
                     /translation="MSALKIMLVTAAAAYGAIVVLMFLFQRNLQYHPENKGLTPESVG
                     LSGVEDMTIATPDGERIRAWHLPAPEGRPTILFFQGNAGEIGDRPARMQFYGDNGFGA
                     LFVSYRGFGGSSGTMSEAGLVSDGVAAYDWLTAQGVSADRILVVGESLGTGVAVQLAA
                     QRPVAALALEAPYTATVDVGAEIYWWLPVRLLMKDRFLSRDHIRNVRAPVLIVHGDAD
                     RLIPVAHGQRLFELASEPKEIEIIPGGRHDVILEPEVWTREVAFFERVLARQ"
     gene            complement(1306..>2398)
                     /locus_tag="AC16A0_10700"
     CDS             complement(1306..>2398)
                     /locus_tag="AC16A0_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3105850.1"
                     /translation="GKTNVPLMLADWQSYNDIYGTTNNPWDLTRSPGGSSGGSAVALA
                     TGMSAIEIGSDIGASIRNPAHYCGVYGHKPTWGVVPYRGHFLPGVVHPTDISVAGPLA
                     RSARDLSAMMKLLAGSDGAEARAMKISLPPVRQTKLKQFRVAVMLTSPESEVDEPVQE
                     LLAKLAAFLAKRVKALSLDARPGFSTGEAMDVYITLLRAATSRRQTEEEFAINAAKVK
                     GFAAGDDSYYAKMLRAYVIPHRSWLMVNERRHQMRLLWDQFFENWDVMLCPAAASAAF
                     PHDHVGERHERTIEVNGKRVATTDQLFWAGYSGCFYLPSTVAPMGLTPQGLPAGVQII
                     ARQYGDFTAIRFAELLEREYGGFVPPPGY"
BASE COUNT          409 a          760 c          798 g          431 t
ORIGIN      
        1 aaccggcaga ttttcgcttg cccgagccca cgcgactgcc ggtgctgaag tatctccctg
       61 actgtatcgg gcaggccatg ctgctggacg ccgacctcgc ggcggcgctc gcggccatcg
      121 aggacgattt gcactgggcg cggtcggcgt cctacagcga tgccgtcctg ggcgagggtt
      181 tctgcgacaa ttacggttgg gccgaactga tcggatccgg cggcttcttt gacggtgacg
      241 acttccgcct cggcttgctg atgctggggc ctgatcggca ctacaaggat cactatcacc
      301 ccgcgccgga actctactgg cccctgaccg gtccttccga ctggaaggcc ggggctggcg
      361 ggtttgtgac gcggtcagcc ggcgagatca tctggcacat tccgtggaag gtgcatgcca
      421 ctgccaccgg cagcacgcca ctcctggctg tctggagctg gacacgggac gcggacacac
      481 ctgcgaagct cgtcggcgca tgagcgcgct gaagatcatg ctcgtcaccg ctgcggcggc
      541 ctatggggca atcgtggtgc tgatgttcct gtttcagcgg aacctccagt accatcctga
      601 gaacaaggga ttgacgccgg agagcgtcgg cctttccggc gtcgaggaca tgaccatcgc
      661 cacgccggac ggagaaagga tccgggcctg gcatctgccg gccccggaag gcaggcccac
      721 catcctcttc ttccagggca atgccggcga aatcggcgac cggcctgcgc gcatgcagtt
      781 ctacggcgac aatggattcg gcgcactctt cgtgtcctat cgcggctttg gcggcagcag
      841 cggcacgatg agcgaggctg gcctggtctc ggacggtgtc gcggcctacg actggctgac
      901 ggcgcagggt gtcagcgccg atcgcattct cgtggtaggc gagtcgcttg gaacaggagt
      961 cgccgtccag ctcgccgcac agcggcccgt tgccgcgctg gcgctggaag cgccctatac
     1021 cgcgacggtc gatgtcggcg cagagatcta ctggtggctt ccggtgcgcc tgctgatgaa
     1081 ggaccgtttc ctgtcacgcg accacatcag gaatgtgcga gcgccggtcc tgatcgtgca
     1141 tggtgatgct gaccggctca ttccggtggc gcatgggcag aggctcttcg aactggccag
     1201 tgagcccaag gagatcgaga tcattcccgg tgggcgtcat gacgtgatcc tcgagcccga
     1261 agtctggacg cgcgaggtgg ccttcttcga acgcgtgctc gcgcgtcagt agcccggcgg
     1321 cgggacaaag ccgccatact ccctctccag aagctcggca aagcggatgg cggtgaagtc
     1381 gccatactgc cgtgcgatga tctgaacgcc ggccggcagg ccttgcggtg tcaggcccat
     1441 gggcgcaacg gtcgagggca gatagaagca gccggagtag ccggcccaga acagctgatc
     1501 ggtggtcgcc acgcgctttc cgttgacctc gatcgtccgt tcatggcgtt cgcccacatg
     1561 gtcgtgcgga aatgcggccg aggccgcggc ggggcagagc atcacgtccc agttctcgaa
     1621 gaactgatcc cacagcaatc gcatctggtg gcggcgttcg ttgaccatca gccaggacct
     1681 gtgcggtatc acataggccc gcagcatctt ggcgtagtag ctgtcatcgc cggcggcgaa
     1741 acccttcacc ttggcggcat tgatcgcgaa ttcctcctcg gtctggcggc ggctggtcgc
     1801 ggcgcggagc agggtgatgt agacgtccat ggcctcgcca gtggagaacc ccggccgtgc
     1861 gtcaagcgac agagccttca cgcgcttcgc aaggaatgcc gcaagcttcg ccaggagctc
     1921 ctgcaccggc tcatccacct cgctctccgg cgaggtcagc atgacggcga cgcggaactg
     1981 cttcagcttc gtctgccgca ccggcggcaa cgagattttc atggcacgcg cctcggcgcc
     2041 atcgctgccg gccaggagct tcatcatcgc cgacaagtcg cgggcagaac gcgccagggg
     2101 accggcaacc gagatgtcgg tggggtgcac cacgcccggc aggaaatggc cacgataggg
     2161 cacgacgccc catgtgggct tgtgtccata gacgccgcag taatgtgcag gattgcggat
     2221 cgaggctccg atgtcggacc cgatctcgat ggccgacatg cccgtggcca gcgccacggc
     2281 cgacccaccc gaggagccgc cgggcgagcg cgtcaggtcc cagggattgt tggtcgtgcc
     2341 atagatgtca ttgtagcttt gccagtcggc aagcatcagc ggcacgttgg tcttgcca
//
LOCUS       DBNGVR010000512         4229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4315609, whole genome shotgun sequence.
ACCESSION   DBNGVR010000512 DBNGVR010000000
VERSION     DBNGVR010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4315609"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="AC16A0_10705"
     CDS             <1..532
                     /locus_tag="AC16A0_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3105851.1"
                     /translation="DSIGNRDGHAQGWRECFDKLTPLVDALRDSDHEINITRLLRHDI
                     GMVFEAFSNPAGLAVWWGPNGFTTTTRSMDFRVGGSWDYIMHGPDGTDYPNHVRYTAI
                     ETNRMIAYDHGTHADHPALFKAIISFVAEGKMTRVNLRLILRDAGQRPHFISFGAVEG
                     GYQNLARLDAWLDARA"
     gene            529..1005
                     /locus_tag="AC16A0_10710"
     CDS             529..1005
                     /locus_tag="AC16A0_10710"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3105852.1"
                     /translation="MTASIQHATLEFENEIAARPHAVFSAYADVGERIGWGTPSDTAT
                     LIYDAADFREGGTDRFRCGPKDDPSIHGTTQYLAIAEDRHIVSIETLATTGQTLAVAL
                     NTIMFLPAASGTRLVVTVQVASFAGKGMIEGYEQGHRTCLANLASLLSWQPAPDKT"
     gene            1064..1591
                     /locus_tag="AC16A0_10715"
     CDS             1064..1591
                     /locus_tag="AC16A0_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3105853.1"
                     /translation="MLTWILAIIAIAILAVLGLAASRPASFRVERAAQIAAAPDKIFS
                     LIDDFHAWPRWSPWEKLDPAMTRSHSGNPSGVGAVYGWSGNKKVGQGRMEITEAERPS
                     RIGLDLEFMAPWKARNRTEFLLRPTDGGTSVSWIMTGSSPFMFRLMGLFMNMDRMIGK
                     DFEAGLANLRKAAEA"
     gene            complement(1588..2637)
                     /gene="trpS"
                     /locus_tag="AC16A0_10720"
     CDS             complement(1588..2637)
                     /gene="trpS"
                     /locus_tag="AC16A0_10720"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3105854.1"
                     /translation="MSTPTSKFKPRVFSGMQPTGNLHLGNYLGAMLNWIHMQDTHECI
                     YCVVDMHAITVWQDPKELNKAIHDVTAAYLACGLDPRKSIIFNQSQVAEHAELAWILN
                     CVARVGWLNRMTQFKEKAGKDREAASVGLYIYPNLMAADILAYHATHVPVGEDQKQHL
                     ELARDIAIKFNNDYAVSIAAHGFDGTFLVPPEPYITGPATRVMSLRDGTKKMSKSDPS
                     DLSRINLTDDAETVARKIRKAKTDTEPLPSEAAGLKGRPEAENLTGIYAALAGEPLEK
                     VLAQYGGGPFSTFKNALADLAVAKLGPIGEETKRLQADPAFVDAVLTDGSARARAIAA
                     PIVRKAREIVGFVVS"
     gene            2751..3635
                     /gene="mutM"
                     /locus_tag="AC16A0_10725"
     CDS             2751..3635
                     /gene="mutM"
                     /locus_tag="AC16A0_10725"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR3105855.1"
                     /translation="MPELPEVETVMRGLKPVLEGQRIETVTLRRADLRFPFPQGFAAR
                     LAGRQILGLSRRAKYILAEIEGGDCLLVHLGMTGRFTVLRPDGSRNLGEFYFETGAGS
                     DAAGAHDHVVMALADGTRLVYSDPRRFGMMDLFPSGETATHKLLRDIGVEPLGNGFHA
                     EYLAEKFKGKSAPLKAALLDQSIVAGLGNIYVCEALFRTGLSPTRKAGTLARGKRVDP
                     RLDVLVRHIRDILGEAIAAGGSTLQDFAGTDGTAGVYQQRFAVYDREGEACTRCSATV
                     RRRVQSGRSTFYCAACQK"
     gene            3643..>4229
                     /locus_tag="AC16A0_10730"
     CDS             3643..>4229
                     /locus_tag="AC16A0_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3105856.1"
                     /translation="MAYEHIIVETRDRVGLVTLNRPKALNALNSALIAELNTALEAFE
                     ADAGIGCIVIAGSEKAFAAGADVKEMADKTYVDAYLGQFLAGWSRLAAARKPVIAAVS
                     GFCLGGGCELAMMCDFIVASETARFGLPEITLGIMPGAGGTQRLPRAIGKAKAMDMIL
                     TGRMMEAAEAERCGLVARVVAPERVLDEALAAAAK"
BASE COUNT          756 a         1344 c         1369 g          760 t
ORIGIN      
        1 cgacagcatc ggaaaccgtg acgggcatgc ccagggctgg cgcgaatgct tcgacaagct
       61 cacgccgctg gtcgatgcgc tcagggactc cgaccacgag atcaacatta cgcgcctgtt
      121 gcggcacgac atcggcatgg tcttcgaggc cttttccaat ccggccgggc ttgcggtgtg
      181 gtggggaccg aacggtttca ccacgaccac caggagcatg gatttccgtg tcggcggcag
      241 ctgggactac atcatgcatg ggccggacgg aaccgactac cccaatcatg tcagatacac
      301 ggcgatcgag accaaccgca tgatcgccta tgaccacggc acccatgctg accatcccgc
      361 cctgttcaag gccatcatca gttttgtggc cgaaggcaag atgacgcgcg tgaacctcag
      421 gctcatcctg cgcgatgcgg gccagcgtcc ccacttcatc agcttcggcg ccgtcgaagg
      481 cggctatcag aatctggcac gcctcgacgc atggctggat gcgcgcgcat gaccgcgtcg
      541 atccagcatg cgacgctcga attcgaaaac gagatagcgg cgaggcctca tgccgtgttc
      601 tcggcctatg ccgatgtggg agagcgtatc ggctggggca ccccgtctga cacggcaaca
      661 ttgatctatg atgccgccga cttccgcgaa ggcggcacgg accgcttccg ctgcggaccg
      721 aaggacgatc cgtcgatcca tggaaccacg cagtacctcg caattgccga ggatcggcac
      781 atcgtgagca ttgaaacgct ggcaaccacc ggacagacgc tggccgtcgc tctcaacacc
      841 atcatgttcc tgccagccgc gtccgggacc cgtctcgtcg tcaccgtgca ggtcgcgtcc
      901 tttgccggca agggaatgat cgagggttat gagcagggac accgcacatg ccttgcaaat
      961 ctggcttcac tcctttcctg gcagccggcg cccgacaaga cgtgagttcc cgcggggtgg
     1021 cggaagggct agactgagcg ccggaccgtc acaggaggat gccatgctca cctggattct
     1081 ggccatcatc gcgattgcca ttctagccgt gcttggactg gccgcatccc gccccgcaag
     1141 ctttcgcgtc gaacgcgcag cacagatcgc ggctgcgccc gacaagatct tttccttgat
     1201 cgatgacttt cacgcgtggc ccaggtggtc gccgtgggaa aagctcgatc ccgccatgac
     1261 gcgcagccac tcgggcaatc ccagcggtgt tggcgccgtc tatggctgga gcggcaacaa
     1321 gaaggttggc cagggccgca tggagatcac cgaagccgag cgcccgtcgc gcatcggcct
     1381 cgacctcgag ttcatggcgc cgtggaaagc gcgaaaccgc accgagttcc tgctgcggcc
     1441 cacggatggc ggaacttccg tcagctggat catgaccggc agcagcccct tcatgttcag
     1501 gctgatgggc ctgttcatga acatggaccg gatgatcggc aaggacttcg aggccggcct
     1561 cgccaacctc aggaaagcgg cagaagccta ggacaccacg aagccgacga tctcgcgcgc
     1621 cttgcgcacg atgggcgcgg ctatggcgcg ggcgcgggcg gagccgtccg tcagaaccgc
     1681 gtcaacaaag gctggatcgg cctgcagccg cttggtctcc tcaccgatcg ggccgagctt
     1741 ggccactgcg agatcggcca gcgcgttctt gaaggtcgag aagggtccgc cgccatactg
     1801 cgccagcacc ttctcaagcg gctcgcctgc cagggccgca tagatgccgg tgaggttctc
     1861 cgcctccggc cggcccttca gtcctgccgc ctcgctgggc agcggctcgg tgtcggtctt
     1921 ggccttgcga atcttccgcg ccaccgtctc ggcgtcgtcg gtgagattga tgcgcgacag
     1981 gtcggaagga tcggatttcg acatcttctt ggtcccgtcg cgcaggctca tcacgcgggt
     2041 ggccggcccg gtgatgtagg gctcgggcgg cacgaggaag gtgccgtcga agccatgcgc
     2101 ggcaatggac acggcatagt cgttgttgaa cttgatggcg atgtcgcgcg ccagttcgag
     2161 gtgctgcttc tgatcctcgc ccactggcac atgggtggcg tgataggcca gaatgtcggc
     2221 ggccatcagg ttcggataga tatagaggcc gaccgatgct gcctcccggt ccttgcccgc
     2281 cttctccttg aactgggtca tccgattgag ccagccgacc cgggccacgc agttgaggat
     2341 ccaggccagt tcggcgtgct ctgccacctg cgactgattg aagatgatgc tcttcctcgg
     2401 atcgagcccg caggccagat aggcggcggt cacgtcatgg atcgccttgt tcagttcctt
     2461 cggatcctgc cacacggtga tcgcatgcat gtcaaccacg cagtagatgc attcatgggt
     2521 atcctgcatg tggatccagt tgagcatcgc gccaaggtag ttgccgagat gcaggttgcc
     2581 ggtgggctgc atgccggaga agacgcgtgg cttgaacttg gaggtggggg tcgacatgtg
     2641 gcaaacctct gggaaatccg gcagggctta tgcccgcaat cggtggccga gaaaagggtc
     2701 cattccttcg cctcttgaaa gcggctgcca aacccggtct aaccgcagcc atgccggaac
     2761 ttccagaagt cgagaccgtg atgcgcggac tgaagcccgt gctcgagggc cagcgcatcg
     2821 agacggtcac cctgcggcgg gccgatctgc gctttccctt tccgcaaggc ttcgcggcgc
     2881 gcctcgccgg gcggcagatc cttggactct cgcgccgggc aaagtacatt ctcgccgaga
     2941 tcgagggcgg cgactgcctg ctcgtccatc tcggcatgac cggccgcttt accgtgctgc
     3001 gccccgatgg gagccgcaat ctcggcgaat tctacttcga gacgggcgcc gggagcgatg
     3061 ccgcgggtgc gcatgaccat gtggtgatgg cgttggccga cggaacccgc ctggtctaca
     3121 gcgatccccg ccgcttcggc atgatggatc tgtttccgtc cggcgaaacg gccacccaca
     3181 agctgctgcg ggatatcggc gtcgaacctc tcggaaacgg cttccacgcg gaatatctgg
     3241 cggagaaatt caagggaaag tcggctcccc tgaaggccgc ccttctcgac cagagcatcg
     3301 tcgcgggcct cggcaacatc tatgtctgcg aggcgctgtt ccgcaccggc ctgtcaccca
     3361 cccgcaaggc cggcacgctg gcgcgcggca agcgggtcga cccccggctc gacgtgctgg
     3421 tgcgccatat ccgcgacatt ctcggcgagg ccatcgcggc gggtggctcg acgctgcagg
     3481 attttgccgg cacggatggg acagccggtg tctaccagca acgcttcgcc gtctatgacc
     3541 gcgagggcga ggcctgcacg cgctgcagtg caaccgtcag aagacgagta cagtcggggc
     3601 gctcgacatt ctattgtgcg gcctgtcaga aataggatgg ccatggctta cgaacacatc
     3661 atcgtcgaga cgcgcgaccg ggtcggcctg gtcacgctca accggcccaa ggcgctcaat
     3721 gcactgaact cagcgctgat cgccgagctc aatacggcac tcgaggcctt cgaggcggat
     3781 gccggcatcg gctgcatcgt gatcgccggc agcgagaagg cttttgcagc cggcgccgac
     3841 gtgaaggaga tggccgacaa gacctatgtc gacgcctatc tgggccagtt ccttgccggc
     3901 tggtcgcggc ttgcggcggc gcgcaagccg gtgattgcgg cagtctcggg cttttgcctc
     3961 ggcggtggct gcgaactcgc catgatgtgc gacttcatcg tcgccagcga gacggcaagg
     4021 ttcgggcttc ccgagatcac cttgggcatc atgccgggcg cgggcggcac acagcgcctg
     4081 ccgcgagcca tcggcaaggc caaggcgatg gacatgatcc tgaccggccg catgatggaa
     4141 gcggccgagg ccgagcgctg cgggctggtt gcgcgggtcg tggcgccgga gcgggtgctc
     4201 gatgaagcgc tggctgcggc cgcgaagat
//
LOCUS       DBNGVR010000513         2249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3138270, whole genome shotgun sequence.
ACCESSION   DBNGVR010000513 DBNGVR010000000
VERSION     DBNGVR010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3138270"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="AC16A0_10735"
     CDS             <1..206
                     /locus_tag="AC16A0_10735"
                     /inference="COORDINATES: protein motif:HMM:NF042682.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HIR3105857.1"
                     /translation="YRAIIEGLLPSLKHSNLETAVEIASLPAMIRGFGHVKEASVARA
                     RLREAELLGQFSGAPPKLKAAAE"
     gene            complement(207..1121)
                     /locus_tag="AC16A0_10740"
     CDS             complement(207..1121)
                     /locus_tag="AC16A0_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3105858.1"
                     /translation="MTARRTAPAFMGARKSKTPQGFTAALFGAPHGTPYPGIDNRIHQ
                     AAPDAFRLALKEDALWLDHWDFDIGGTLLGRGISVAELGNLATTPKQGAKNRKLIEAC
                     TRRILEAGAIPVMFGGDDSVPIPFIQAYSGQSPIVILQIDAHIDWREERYGERMGFSS
                     TMRRASEQEHVWRIVQAGMRGIGSAREAEVRDALDWGARLVTARDIHHHGVGSVLQHI
                     PEDCDCVISFDCDALDLSQMPAVSYPTPGGLTYTQVTDLIAGVAAKARIAGFTMVEFM
                     PRRDPAGTAAFTAARIAANVIGRLQPRS"
     gene            1287..>2249
                     /gene="hemH"
                     /locus_tag="AC16A0_10745"
     CDS             1287..>2249
                     /gene="hemH"
                     /locus_tag="AC16A0_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3105859.1"
                     /translation="MTVGKIGVLLINLGTPEATSYWPMRRYLKEFLSDRRVIEVNRAV
                     WWLILNGIILTTRPRKSGHAYEQIWNKERDESPLKTITRSQAEKLAAKLGDCPQVIVD
                     WGMRYGLPPTGERIKALKEQGCDRILLFPLYPQYSATTTATALDKAYDALKDMRWQPA
                     IRTVPPYFDDPAHIEALAISLDRHLKGLSWTPDLILASFHGLPQDYFMDGDPYHCHCM
                     KTARLLAARMGLPRDKLQVVFQSRFGKAEWLQPYAQQTVEELPGQGVKKLLMITPGFA
                     SDCVETLEEVAIGLKETFEEAGGEKFSVVPCLNDSDPSITMMEEI"
BASE COUNT          388 a          731 c          737 g          393 t
ORIGIN      
        1 actatcgcgc cataatcgaa ggcctcttgc cgtccttgaa gcactccaac ctcgagacgg
       61 cggtcgagat cgcttcgctg cctgcaatga tccgtggctt cggccatgtg aaggaagcca
      121 gtgtggcaag ggcgcggctg cgcgaggcgg aattgcttgg gcagttttcc ggtgcgccgc
      181 cgaaactcaa ggcggccgcc gagtgatcag ctgcgcggct gaagacggcc gatcacgttg
      241 gcggcaatgc gggcggccgt gaaggcggca gtgccggcgg ggtcgcgccg cggcatgaat
      301 tccaccatcg tgaatcctgc gatgcgggcc ttggcggcga cccccgcgat cagatcggtg
      361 acctgcgtat aggtcagccc gccgggagtc ggataagaca ccgccggcat ctgggagagg
      421 tcgagcgcgt cgcagtcgaa ggagatcacg cagtcgcagt cttccgggat gtgctggagc
      481 acgctgccca caccgtggtg gtggatgtcg cgcgcggtga ccagccgggc gccccagtcc
      541 agcgcatcgc gcacctcggc ctcccgcgcg ctgccgatgc cgcgcatgcc ggcctggacg
      601 atgcgccaga cgtgttcctg ctcggacgcg cgccgcatgg tggacgagaa gcccatgcgc
      661 tcgccgtatc tttcctcgcg ccagtcgatg tgggcgtcga tctgcaggat cacgatgggc
      721 gactggcccg aataggcctg gatgaagggg atcggcacgg agtcatctcc gccgaacatc
      781 acgggaatgg cgcccgcttc gaggatgcga cgggtgcagg cctcgatgag cttgcggttc
      841 tttgccccct gtttcggcgt ggtggcgagg ttgccgagtt cggccacgct gatgcccctg
      901 ccgagcaggg ttccaccaat gtcgaaatcc cagtggtcca gccacagggc atcttccttc
      961 agggccagcc ggaaggcgtc cggtgccgcc tggtggatgc gattgtcgat gccgggatag
     1021 ggcgtgccat ggggcgcgcc gaagagtgcg gcggtgaagc cctgtggcgt cttcgatttc
     1081 cgggccccca tgaaggccgg agctgtccgt cgtgccgtca tggcctttcc ttgccgcagg
     1141 acgcggccgc tggcaatgct ggcggatgat cctcccgccc cgccgggacg ttatcacaac
     1201 ctctagcctt ccccaattcc cggcggtaac aacacccgat gacagctgtt cctgcgcttg
     1261 gcccgctccc gcccgggcat cctccggtga cggtcgggaa aatcggcgtt ctcctgatca
     1321 acctcggcac gcccgaggcc acctcctatt ggccgatgcg ccggtatctg aaggaattcc
     1381 tgtcggaccg gcgggtcatc gaggtcaatc gcgcggtctg gtggctcatt ctcaacggca
     1441 tcattctcac cacccggccc cggaaaagcg gccatgccta cgagcagatc tggaacaagg
     1501 agcgcgacga gtcgccgctc aagaccatca cccgcagcca ggccgagaag cttgcggcaa
     1561 aactgggcga ctgcccccag gtgatcgtcg actggggcat gcgctacggc ctgccgccga
     1621 ccggcgagcg gatcaaggcg ctgaaggaac agggttgcga ccgcatcctg ctctttccgc
     1681 tctatccgca gtacagcgca accaccacgg ccaccgccct cgacaaggcc tatgacgccc
     1741 tgaaggacat gcgctggcag ccggcgatcc gtaccgtgcc gccttacttc gacgatccgg
     1801 cccatatcga ggccctggcg atatcgctgg accgtcacct gaagggcctg agctggacgc
     1861 ctgacctcat cctcgcctcg ttccacggcc tgccgcagga ctacttcatg gacggcgatc
     1921 cctatcattg ccactgcatg aagacggcac ggcttctggc ggccaggatg ggcctgccca
     1981 gggacaagct gcaggtggtc ttccagtcgc gcttcggcaa ggcggagtgg ctgcaaccct
     2041 atgcccagca gacggtcgag gaactgcccg gccagggggt gaagaaactc ctcatgatca
     2101 cgccgggctt cgcctccgac tgcgtggaaa cgcttgagga ggtggcaatt ggcctgaagg
     2161 aaacgtttga ggaggcgggc ggcgagaagt tctccgtggt gccctgcctc aatgattccg
     2221 acccctcgat taccatgatg gaggagatc
//
LOCUS       DBNGVR010000514         1769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4907938, whole genome shotgun sequence.
ACCESSION   DBNGVR010000514 DBNGVR010000000
VERSION     DBNGVR010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4907938"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="AC16A0_10750"
     CDS             complement(<1..634)
                     /locus_tag="AC16A0_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3105860.1"
                     /translation="MTLPHIPDPVPGDAKAADLIESVIVPRARDLGGFEVRRALPAAE
                     RQMVGPFIFFDQFGPVLMRAGQGIDVRPHPHIGLATVTWLFDGSIYHRDSLGSQQPIA
                     PGELNWMTAGRGIVHSERTDEAELARDRHVFGIQSWVALPKAFEETAPAFEHVAMERL
                     PLIEDRGVTVRVVAGSVFGSTSPVRTFSDLFYADVRLSAGSALPLPADHEE"
     gene            complement(702..>1769)
                     /locus_tag="AC16A0_10755"
     CDS             complement(702..>1769)
                     /locus_tag="AC16A0_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR3105861.1"
                     /translation="MDGGAPVGEAWNFDQDNRKPLPRDARLPTRRRFEPDGVTQGVVT
                     LVGERFASHFGSLAAFGWAVTRPDALEALDQFITVCLPSFGDYQDAMKQGNAFLHHAV
                     ISPYLNVGLLTAREVCARAEKAWRTGAAPLNAVEGFIRQILGWREYVRGLYWLKMPGY
                     AESNALHATRDLPGFYWTGETTMNCLAQVIGDTQRHAYAHHIQRLMVTGNFALLAGLA
                     PRKVEAWYLLVYADAFEWVELPNTHGMALFADGGVMASKPYAASGAYIDRMSDYCTRC
                     AYDPKQKTGEKACPFNYLYWDFLARNEERLKGNPRLAMPYRTLARMDAERRAAISADA
                     RRFLSTLDAPAHGADQLVLEI"
BASE COUNT          357 a          558 c          543 g          311 t
ORIGIN      
        1 gttcctcgtg gtctgcgggc agcggcaagg cactgccggc cgacaaccgc acatctgcat
       61 agaacagatc ggaaaaagtc ctcaccggcg aagtggagcc aaagaccgag ccggccacga
      121 cgcgcactgt caccccccga tcctcgatca aaggaaggcg ttccatcgcg acatgttcaa
      181 aggccggtgc ggtttcctcg aacgcctttg gcaaggccac ccagctctgg atgccgaaga
      241 cgtggcggtc gcgggccaat tccgcctcat ccgtacgttc ggaatgcacg atgccccggc
      301 ccgccgtcat ccagttgagc tcgccgggcg cgatcggctg ttgggaccca aggctgtccc
      361 ggtggtagat cgagccgtca aagagccagg tgacggttgc cagtccgata tgaggatggg
      421 gacgcacatc gatcccctgc cccgcccgca tcagcaccgg cccgaactgg tcgaagaaga
      481 tgaaggggcc gaccatctgg cgctcggctg ccggcaatgc gcgccgcacc tcgaagccgc
      541 caagatcgcg ggcacggggc acgatgacac tctcgatcag atcggccgcc ttcgcgtcac
      601 ccggcacagg atcaggaatg tggggaagtg tcatggcggc gcctcctctg ctggcagacc
      661 ctgtggaagg atcccgatca aacttgatgt cctcgtcgcg actagatttc aagcaccagc
      721 tgatccgccc cgtgggccgg cgcgtcaagc gtcgagagaa accgcctggc atccgccgaa
      781 atggcggcac ggcgctcggc gtccatgcgc gccagtgtcc gatagggcat ggcgagccgg
      841 ggattgccct tcaggcgttc ctcatttctc gctagaaaat cccaatacaa gtagttgaat
      901 ggacacgctt tctctcccgt cttctgcttc ggatcatagg cacagcgcgt gcagtaatcc
      961 gacatgcgat cgatataggc accggatgcc gcatagggct tggacgccat cacgccgcca
     1021 tcggcaaaga gcgccatgcc gtgggtattt ggcagttcga cccattcgaa cgcatcggca
     1081 tagaccagaa ggtaccaggc ttcgaccttg cggggcgcga ggccggcgag aagcgcgaag
     1141 ttgccggtga ccatcaggcg ctggatgtga tgggcatagg cgtggcgctg cgtatctccg
     1201 atgacctggg caaggcagtt cattgtggtc tccccggtcc aatagaagcc cggaagatcg
     1261 cgcgtcgcat gcagggcatt gctttcggca tagccaggca ttttcagcca gtagaggccg
     1321 cgcacatatt cgcgccagcc aaggatttgg cggatgaacc cttccacggc attgagtggt
     1381 gcagcgccag tacgccaagc cttctctgcg cgggcacaga cttcccgcgc agtcagaagg
     1441 ccgacgttga gatagggcga gatgacggcg tggtgcagaa aggcattccc ttgcttcatc
     1501 gcatcctgat aatcgccgaa actcggcaga caaaccgtga tgaattgatc gagagcttcc
     1561 agcgcatccg gccgggtcac ggcccagccg aaggccgcaa gcgatccaaa gtgactggca
     1621 aagcgttccc cgaccagcgt caccacgcct tgcgtcacgc cgtcgggttc gaaacgccga
     1681 cgtgttggca gccttgcatc gcggggcagc ggcttgcggt tgtcctgatc gaagttccat
     1741 gcctctccga ccggcgcacc tccatccat
//
LOCUS       DBNGVR010000515         4113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2747051, whole genome shotgun sequence.
ACCESSION   DBNGVR010000515 DBNGVR010000000
VERSION     DBNGVR010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2747051"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..818)
                     /locus_tag="AC16A0_10760"
     CDS             complement(57..818)
                     /locus_tag="AC16A0_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3105862.1"
                     /translation="MDLLHIVLLFAGGLLAGLINVMAGGAGFMTFPLLLAAGLSEIEA
                     NASNFVAVLPANVVGTVVYRSELASVRRHLWLRLLLAAAGGVIGSTILIMTGEAAFEK
                     VIPWLLLFATLSFALGPWLKQKLERDFNFDGSRWLWLSFVLEFIVYVYGGYFGLGMGI
                     VMFAIYSIFSQMNIHQANAIRNITITLMTMISIAIFAQAGVIRWIPSLVMMSGAVIGG
                     YVTARFAKRMPPSVIRNGILVWAVCLTTLAFWRYL"
     gene            879..1448
                     /locus_tag="AC16A0_10765"
     CDS             879..1448
                     /locus_tag="AC16A0_10765"
                     /inference="COORDINATES: protein motif:HMM:NF016062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HIR3105863.1"
                     /translation="MADPVQCSEETCYPVRMWTNVIILSLATLQRIVEFVIATRNTRR
                     QVARGGFVIGPNLQPVMILLQTAWLIGLWYLAYRLEVSWPWLFAYIGLEAARGWIVAL
                     LGSQWTSRIVVNASEPFEGSRPFSIFREPNYVIIGLEMFILPMVFGLWWWGAIFAALY
                     AALIYWKVIAENKAVKPLTEPPESDATTA"
     gene            complement(1461..1931)
                     /locus_tag="AC16A0_10770"
     CDS             complement(1461..1931)
                     /locus_tag="AC16A0_10770"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR3105864.1"
                     /translation="MRKLTLEIAAAAALTLGAILASATGVSASDVLVKDAYARASATP
                     VAKAGAVYVSITNQGTSADRLLAVSSPVASMATLHRTIKDGDIMRMEEAGPVDLQPNG
                     TLTMEPGGLHVMLMGLKAPLKQGEEVELTLVFETAGPVTVKVPVGSAVAGTKTE"
     gene            1930..2658
                     /locus_tag="AC16A0_10775"
     CDS             1930..2658
                     /locus_tag="AC16A0_10775"
                     /inference="COORDINATES: protein motif:HMM:NF018420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HIR3105865.1"
                     /translation="MTQVLIRKWAVVWRKVRDTASRVVLAIPFLAQAEAKAQEPVAVE
                     LVLALDSSASVDRREFQLQLEGLALAFRDPEVLQAVENLKPLGAAIAVLQWGAPGETR
                     IVLPFTPVRSAREAKAFGFQIGLIRRWQWASSTSIATALDDSRMLIESNDYEGFRRII
                     DVSGDGPDNGGADLEAARKRALAAEITVNGLPIMADDSALDRYYEDKVIAGATAFIEP
                     ARDFEDYVRAIREKLLRELRPLGS"
     gene            complement(2655..>4113)
                     /locus_tag="AC16A0_10780"
     CDS             complement(2655..>4113)
                     /locus_tag="AC16A0_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIR3105866.1"
                     /translation="VGKLQAEGFDVEEGSGGMPTAFAATWSNGPGPTIMAYAEYDAVP
                     GNCQIADTYRAPRHGLSRFAGGHTDPHSALGIGALVGALAAKAAMQAHGISGTIRFMG
                     EPAEKVRGSKPIHAARGYYDGLDAILSFHPFYMMPYCNTARWDTHCGPYYAAIYEFLC
                     ESPETWLQGSGGAGGPIPAAHTMARAPGANDAVVAMYTLSKMMRDHMLPHSGTWTLNE
                     TILASGQATADNLAAQMAMIMFAARAPDAAMLQNIHDVLDRNAAAAAAAAHCTVRKHW
                     VSKSKPGLANHAMAEITFRNLERAGAPRYGADAVAMAQALQRELGLEAMPSPFADELS
                     QLITPQEAERRIRLQMPAWQTHYTSDDYTDMTWFAPTARFYVGRAVLKAPPGFAYPEW
                     ALNALGGIPACIDPTIATAGKTIAATILDLMLDRDTLARAKAEFADRKAKESDPVPWC
                     DYEPPLDFPWPEYVETPRGREWWIPATSADRALAR"
BASE COUNT          739 a         1304 c         1369 g          701 t
ORIGIN      
        1 gggcatgctg ctgatcgcga tcaccggcct ctatgccgcg caccgcgaag gccgcgtcaa
       61 agatagcgcc agaatgccag tgtcgtgagg cagacggccc agacgaggat gccattgcgg
      121 atgacactgg gaggcatgcg cttggcgaag cgcgccgtga catagccgcc gatcaccgcg
      181 ccactcatca tcacgaggga gggaatccag cgaatgacgc cggcctgagc gaagatcgcg
      241 atgctgatca tggtcatcag cgtgatggtg atgttgcgga tggcatttgc ctggtgaatg
      301 ttcatctgcg agaagatcga atagatcgcg aacatcacga tgcccatgcc gaggccgaag
      361 tacccgccat aaacatagac gatgaactcc agcacgaagg acagccacag ccagcggctg
      421 ccgtcgaagt tgaagtcgcg ctccagcttc tgcttcagcc acgggcccag ggcgaaggag
      481 agcgtcgcga acagcagcag ccaggggatc accttctcga aggcggcctc gccggtcatg
      541 atcaggatgg tggagccgat gacgcccccc gcggccgcca gcagcagccg caaccacaaa
      601 tggcgccgca cgctggccag ttcgctgcga tagacgacgg ttcccaccac attggcgggc
      661 aggaccgcca cgaaattcga ggcattggcc tcaatctcgc tgaggcccgc ggccagcagc
      721 aggggaaagg tcatgaagcc cgcgccgccg gccatgacgt tgatcaggcc ggccaggagg
      781 ccacccgcga aaagcaagac gatatgcagc agatccatgc gtgaagccct gccgttccat
      841 agacccgctg ccacccgggc cacaatgggc ggaaccgcat ggccgacccc gtgcaatgtt
      901 cggaggaaac gtgctatcca gtccgcatgt ggaccaacgt gatcatcctc tcccttgcca
      961 cgctgcagcg catcgtggag ttcgtcatcg ccacccgcaa cacgcggcgg caggttgccc
     1021 ggggcggctt cgtcatcgga ccgaacctgc agccggtgat gatcctcctg cagacggcct
     1081 ggctgatcgg cctgtggtat ctcgcctacc ggctggaggt gagctggcct tggcttttcg
     1141 cctatatcgg cctcgaggcg gcgcggggat ggatcgtggc cctgctgggc agccagtgga
     1201 cctcgcgcat cgtggtcaat gccagcgaac ctttcgaggg gtcgcgcccc ttcagcatct
     1261 tccgcgagcc caactacgtg atcatcgggc tggaaatgtt catcctgccc atggtcttcg
     1321 gcctgtggtg gtggggcgcg atcttcgccg ccctctacgc agcgctgatc tactggaaag
     1381 tcatcgcgga gaacaaggcg gtcaagccgc tgaccgaacc gccggaatcg gacgcgacga
     1441 cggcctgatc gtgcgcgagg ctattcggtc ttggtgcctg cgactgcaga accgacgggc
     1501 accttgaccg taacgggtcc agcagtttcg aagaccagcg taagctcgac ctcctcgccc
     1561 tgcttgagcg gcgccttcag acccatcagc atgacatgca atccgccggg ctccatggtg
     1621 agggttccgt tcggctggag gtcgacaggg cctgcttcct ccatgcgcat gatgtcgcca
     1681 tccttgatgg tccggtgcag cgtcgccatg gacgcgaccg ggctcgagac cgccaggagc
     1741 cggtcggccg acgttccctg gttggtgatc gagacataga ccgcacccgc cttcgccaca
     1801 ggcgtggccg acgcgcgggc ataggcatcc ttcaccagaa cgtcactggc gctgacgccg
     1861 gtggcggatg ctaggatggc accgagcgtg agggcagcgg cggcggcgat ttcgagcgtg
     1921 agtttgcgca tgacacaagt cttaatccgg aaatgggctg tcgtatggcg gaaggtgcgc
     1981 gacactgcgt cacgcgtggt tctggcgatc ccgtttctgg cacaagctga ggccaaggcg
     2041 caggagccgg tggccgtcga actggtcctc gcactcgaca gctcggcgag tgtggaccgg
     2101 cgggaattcc agctgcagct cgagggtctc gcattggcgt tccgcgatcc cgaggtgctg
     2161 caggcggtgg agaacctcaa gccccttggg gcggccatcg ccgtgctgca atggggcgca
     2221 cccggagaga cgcggatcgt gcttcccttc acgccggtcc gctcggcccg cgaagccaag
     2281 gccttcggct tccagatcgg gctgatccgc cgctggcaat gggccagttc cacctcgatc
     2341 gccaccgcac tcgacgacag ccgcatgctg atcgagtcaa acgactacga aggattccgc
     2401 cgcatcatcg acgtctcggg ggacgggccg gacaatggcg gcgcggacct cgaggcggcg
     2461 cggaaacgtg cgctcgccgc cgagatcacc gtgaacggcc tgcccatcat ggccgatgac
     2521 tccgcgctcg accgctacta cgaagacaag gtgatcgccg gggccaccgc cttcatcgag
     2581 cccgcccgcg atttcgagga ctatgtgcgc gccatccgcg aaaaactgtt gcgggagctc
     2641 aggccgctgg gctcttagcg cgccagcgcc cgatcggccg acgtggcagg aatccaccac
     2701 tcccgtcccc ggggagtctc gacatactcc ggccacggaa aatcgagcgg gggttcgtag
     2761 tcgcaccagg gcaccgggtc tgattccttc gccttccggt ctgcgaactc ggccttggca
     2821 cgcgccaggg tatcccgatc cagcatcaga tcgaggatgg tggcggcgat ggtcttgcct
     2881 gccgtggcga tggtcgggtc gatgcaggcg ggaattccgc ccagggcatt gagcgcccat
     2941 tcgggatagg caaatccagg cggcgccttc agcacggcgc gcccgacata gaaacgcgcc
     3001 gtcggcgcaa accaggtcat gtccgtgtag tcgtcggagg tgtagtgggt ctgccaggcg
     3061 ggcatctgga ggcggatgcg gcgctcggcc tcctgcggcg tgatgagctg ggacagttca
     3121 tccgcgaaag gcgagggcat ggcctcgagg ccgagctcgc gttgcagggc ctgggccatg
     3181 gctacggcat cggcgccata gcgcggcgcg cccgcccttt ccagattgcg aaaggtgatc
     3241 tccgccatgg catgattggc gaggccgggt ttcgacttcg atacccagtg cttccggacg
     3301 gtacaatgcg cggccgcggc ggccgccgcg gcgttgcggt cgagaacgtc atggatgttc
     3361 tgcagcatgg cggcatcggg cgcgcgtgcg gcaaacatga tcattgccat ctgggcggcg
     3421 aggttgtcgg cggtcgcctg gccgctggcg agaattgtct cgttcaatgt ccaggtcccg
     3481 ctgtgcggca gcatgtggtc gcgcatcatc ttggacagcg tgtacatcgc caccactgcg
     3541 tcattcgcac ccggcgcgcg cgccatggtg tgagcggcgg gaatgggacc gccggcacct
     3601 cccgatccct gcagccaggt ttccgggctc tcgcagagga actcgtagat cgcggcatag
     3661 taggggccgc aatgcgtgtc ccagcgcgct gtattgcaat agggcatcat gtagaaggga
     3721 tggaaggaca ggatggcgtc gagtccatca tagtaaccgc gcgccgcatg gatgggcttc
     3781 gatcctctca ccttttcggc cggctcgccc atgaagcgga tggtgccgga aatgccgtgg
     3841 gcctgcattg ccgccttggc agccagtgcg cccacgagcg cgccgatgcc cagggccgag
     3901 tgcgggtcgg tatggccgcc tgcaaagcgc gacaggccat ggcggggggc gcgataggtg
     3961 tcggcgatct ggcaattgcc tggaacggca tcatactcgg cataggccat gatggtggga
     4021 ccgggcccat tggaccaggt cgccgcaaag gccgtgggca tgccgccgct gccctcctcc
     4081 acatcaaatc cctcggcctg cagctttccg aca
//
LOCUS       DBNGVR010000516         2825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_4316698, whole genome shotgun sequence.
ACCESSION   DBNGVR010000516 DBNGVR010000000
VERSION     DBNGVR010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_4316698"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="AC16A0_10785"
     CDS             complement(<1..441)
                     /locus_tag="AC16A0_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3105867.1"
                     /translation="MKKLLIALAATTAMATAAQAEGELNIYNWVEYTSPEMIAKFEKE
                     TGIKVTIDTYDTNETLLAKLKAGGTGYDVVMPSQHFVEIMVKEGLLQDTGVGQMANYK
                     NLDPRWQKPSWDPEARYCAPWQWGTASVAYRKDLYGKEMTSLKEF"
     gene            complement(469..1269)
                     /locus_tag="AC16A0_10790"
     CDS             complement(469..1269)
                     /locus_tag="AC16A0_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105868.1"
                     /translation="MRQPVGHYPGLRLWTALFFFFLYAPMVVLVAYSFNVNKLAMIWG
                     GFSTRWYVKLFTTGGIGEAAMNSLLVAVIATSIAVAIAIAAALVLVREAGFRGADLSN
                     ALISMPLIVPEIVTAVATLMFFVMLKNWLGFDLGLGNIIIAHCVFCIPFAFMPIRARL
                     QDMDLALEQAARDLYADAWKAFRFVTLPLLMPGVVAGAMLAFVISLDDFIITQMISGP
                     GTTTLPVYIYSMIRNGITPEVNAASTILIAVSTALVTVYWYVSRPKKT"
     gene            complement(1269..2159)
                     /locus_tag="AC16A0_10795"
     CDS             complement(1269..2159)
                     /locus_tag="AC16A0_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105869.1"
                     /translation="MLRSTTSNWFGVAPAWLVIGIFGLLPMFIMLAISFMVQDPYGGV
                     EWGFSTEAYVQFLFERDLDDSLILNTIYVNIVARSIGLAAMTTILCLIIGLPVAYFIA
                     RQPEDKRNLYMLLITIPFWTNLLIRTYAWILILTRNGVVDARLQWFGFEPGTFDLLYT
                     NTAIFIGLVYSYLPFMVLPLYASLEKMDFRLIEAASDLYASKWQAARHVIIPLAKPGI
                     VAGSLLVFIPSIGAFIAPELLGGGKRMMLGSLIYFQFATARNWPFGAAVSILLMAFVM
                     IALLVYARAAKGQGLPAGGH"
     gene            complement(2159..>2825)
                     /locus_tag="AC16A0_10800"
     CDS             complement(2159..>2825)
                     /locus_tag="AC16A0_10800"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105870.1"
                     /translation="TQLSGGQIQRAALARALAPSPAVLLLDEPLSALDLKLRQAMRSE
                     LKTLQRETGITFIFVTHDQEEALTMSDRIAVMSAGKIQQIGAPLDIYESPVNHFVADF
                     IGDSNFIEGAIDTIKGDAAVVKTPDGIAIAARMNGPHKAGDRIAVALRPEKILLGAGK
                     LKGEVREAVYLGTSTSYVIALGRTVTLSVREANDLSGKARFAVGDTVKLDIPAGAARM
                     LAD"
BASE COUNT          560 a          829 c          928 g          508 t
ORIGIN      
        1 gaactccttc agcgaggtca tttctttgcc atagagatcc ttgcgatagg cgaccgatgc
       61 ggtgccccac tgccagggcg cgcaatacct ggcttccgga tcccagctcg gcttctgcca
      121 gcggggatcc aggttcttgt agtttgccat ctggccgacg ccggtgtcct gcagcaggcc
      181 ttccttgacc atgatctcga cgaagtgctg gcttggcatc accacgtcat agcccgtgcc
      241 gccggccttg agcttggcca gcagcgtctc gttcgtgtcg taggtgtcga tggtcacctt
      301 gatgccggtt tccttctcga acttcgcgat catctcgggc gaggtgtatt cgacccagtt
      361 gtagatattg agctctcctt cggcctgggc cgcggtggcc atggcggtgg tggcagcaag
      421 agcgatcagc agtttcttca tgtgacgttt ccctttctgt ttggagtttc aggttttctt
      481 cggccgcgag acataccagt agaccgtcac cagcgcggtg gagacggcaa tcaggatggt
      541 cgacgcggca ttcacttccg gcgtaatgcc gttccggatc atggaataga tgtagaccgg
      601 gagcgtggtg gtgcccggcc ccgatatcat ctgtgtaatg atgaagtcgt caagcgatat
      661 cacgaaggcc agcattgcgc cggccaccac gccgggcatg agaagcggca gggtcacgaa
      721 ccggaaagcc ttccaggcgt cggcgtagag gtcgcgcgcc gcctgttcca gcgcaaggtc
      781 catgtcctgg agccgtgccc ggatcggcat gaaggcgaag gggatgcaga agacgcaatg
      841 cgcaatgatg atgttgccca gccccaggtc gaagccgagc cagttcttga gcatgacgaa
      901 gaacatcagc gtggcaactg ccgtcacgat ctccggaacg atgagcggca tggagatcag
      961 cgcattcgac aggtccgcgc cgcggaaccc ggcctcgcgc acgaggacca gtgcagcggc
     1021 gatggcaatg gccaccgcga tgctggtggc gatcaccgcc accagcagcg aattcatcgc
     1081 cgcctcgccg atgccgccgg tggtgaacag cttgacgtac caccttgtcg agaagccgcc
     1141 ccagatcatg gccagcttgt tgacgttgaa ggaataggcg accagcacca ccattggcgc
     1201 atagagaaag aagaagaaca acgccgtcca caggcgcagg cccggatagt gcccgacggg
     1261 ctggcgcatc agtggcctcc tgccggcagg ccctggccct tggcggcccg ggcatagacg
     1321 agaagcgcga tcatcacgaa ggccatgagc aggatggaga cggcggcacc gaagggccag
     1381 ttccgcgccg tggcaaactg gaagtagatg aggctgccca gcatcatgcg cttgccgccg
     1441 cccagaagct cgggtgcgat gaaggcgccg atggacggaa tgaagacgag cagcgagccc
     1501 gcgacaatgc cgggcttggc aagcggaatg atgacatggc gtgcggcctg ccacttgctg
     1561 gcgtagagat cgctggccgc ctcgatgagc cggaagtcca ttttctctag acttgcatag
     1621 agcggcagca ccatgaaggg caggtagctg tagaccagtc cgatgaagat cgctgtgttg
     1681 gtgtagagga gatcgaaggt gccgggctcg aagccgaacc actgcagccg cgcatcgacc
     1741 acgccattgc gggtgaggat caggatccag gcataggtgc ggatcagcag attggtccag
     1801 aagggaatgg taatcagcag catgtagagg ttgcgcttgt cctccggctg gcgcgcgatg
     1861 aaataggcca ccggcaggcc gatgatcagg cagaggatgg tggtcatcgc ggcaagcccg
     1921 atcgagcggg cgacgatgtt cacatagatg gtgttgagga tcagcgagtc gtcgagatcc
     1981 ctctcgaaaa ggaattgcac ataggcctcg gtggagaacc cccactccac gccgccgtag
     2041 ggatcctgca ccatgaagga aatggccagc atgatgaaca tgggcagcag gccgaagatg
     2101 ccgatcacca accaggcggg tgcgacgccg aaccaattgg atgtggtgga gcgcaacatc
     2161 agtcggccag catgcgcgcg gcacccgcgg ggatgtccag cttcaccgtg tctccgacgg
     2221 caaagcgcgc cttgcccgaa aggtcgttgg cctcgcgcac cgaaagcgtg accgtcctgc
     2281 caagggcgat gacatagctg gtactggtgc cgaggtagac ggcctcgcgc acctcgccct
     2341 tgagcttgcc ggcgccgagc aggatcttct cgggcctgag cgcaacggcg atgcggtcac
     2401 ccgccttgtg cgggccattc atgcgcgcgg caatggcgat gccatccgga gtcttgacca
     2461 ccgccgcgtc gcccttgatc gtgtcgatcg cgccctcgat gaaattgctg tcgccgatga
     2521 agtcggccac gaagtgattg acaggacttt cgtagatgtc gagcggtgcg ccgatctgct
     2581 ggatcttgcc cgccgacatc accgcgatcc tgtccgacat ggtgagcgcc tcttcctgat
     2641 cgtgagtcac gaagatgaag gtgatgccgg tttcccgctg cagcgtcttg agctcgctgc
     2701 gcatcgcctg gcgcagcttg agatcgagcg ccgagagcgg ctcgtcgagc agcagcacgg
     2761 cgggggaagg ggcgagcgcc cgtgccagcg ccgcgcgctg gatctggccg ccgctgagct
     2821 gggtg
//
LOCUS       DBNGVR010000517         5114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2747450, whole genome shotgun sequence.
ACCESSION   DBNGVR010000517 DBNGVR010000000
VERSION     DBNGVR010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2747450"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="AC16A0_10805"
     CDS             <1..613
                     /locus_tag="AC16A0_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105871.1"
                     /translation="GMLLAIPIGLVQVTGPSWLAFIARWFCTIIRGTPLLIQLWLIYY
                     GIGSLFPQIGAAYPGFRENFMWLIRLDSFYYALFAFNIRFAGYEGEIMRGAFLGVPRG
                     ELEAARAFGMSPWKVLTRVWFPRAVRLVLPTLAGELVLQLKSTPIAFTVAVMDLMGVT
                     TKIRQDTFRIFEPLMLLAVIYMCLTFVIVWAFGFLERMVPQKR"
     gene            610..1776
                     /locus_tag="AC16A0_10810"
     CDS             610..1776
                     /locus_tag="AC16A0_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014760972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HIR3105872.1"
                     /translation="MIEDLLIPHRNELTAIRHDLHMHPEMLFEEVRTSAIVARELARL
                     GFKVTTGLAKTGVVGTLSNGSSGRSIGIRADMDALPIHETTNLPYASKYPGKMHACGH
                     DGHTTMLLGLARYLAESRNFDGTVHLIFQPSEEDEGGALRMIEDGLFKRFPCDAVFAL
                     HNIPGEEPGQVIVRPGPITAAVDIVGITVRGLGGHGAVPQKAIDPIVAASSLVIALQS
                     VISRNIDPLDPAVITVGSFNAGSMATVIPGEAKLLVGVRTCTRQVRELILKRIEDLAK
                     GQARSFGCEADVQIGDGSWSYPAGANSAPEAALVRAVALEMGQDPAKVDMRGPFMFSE
                     DFAYMQELVPSCYFGIGNGSGPMLHDSSYNFNDELLVKGPAFWARLVAKALPQS"
     gene            complement(1767..4745)
                     /locus_tag="AC16A0_10815"
     CDS             complement(1767..4745)
                     /locus_tag="AC16A0_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3105873.1"
                     /translation="MQQTLIESLKTQRETSLKASAGGAPLRTSNPFFGVGPITDGALD
                     EADSRLTRLAFEHWLETNYRKLDDTGKDIGPFTVAEIARSMHRGYPADKFLLDMMREI
                     HRYFRFPRSNKMAVGLGGGHSGFTVCALHLLTCNDPSQHIYVDTVAPESEEAKASGFF
                     RQSWGAQIVEMLSHARQGDPGRVHFAKTEGGIPSSDTLERLGIKVFFGVGHETTGATC
                     YTKGEVTQLLDWIDRNPGEHHAVLDATSMLGAMPWGEDIVRQVMSKCCLFMPFQKAIG
                     GVSGYFIVSFTPHALALVEENQKAPSWAIPRQLKLAAPADARKPLTGTRSVKLGPFYD
                     PDQDKMLGGVINTFSTLAFAETTFGLLRVERKIGDVTELNRRSVANRAAVNEWMSNSS
                     LFELGVENPEARGAAVTLIKVKDPDIADAAIQSRIVARSKQLLAYDGITHPNGEHEAG
                     LDVARYINAFPGTPGDYRAWIGGIRPLEDVTALLDNLKYAYHRAKIVVLEEELAKLGV
                     TFPAPPEVGSRQRKDDPDRAYRVLICDLIGLKFDARGKPDPGAVQAHIESQGGVFHLG
                     PPGDGKKLAKGKIHFFYMPQLSTAAEILPLTDKGQFDAVIAAATFLPKDAVFPLGGVR
                     IGAGTGNMGSASWGGGNGEGGVAPLMNTPSFNSRATAHMAMKALLKVMPDLPVDELHR
                     RTIAGQFDTGRNLKEFPTEKLEGKKIAIIGYGNIGREVAKLAQAFGMRVAVHARPRHK
                     DWIESEGFEFAPTPEDAARGADVISPHTGLGPLNAATGKFANANVVNAAVLAQLNDGA
                     IVINYDRGECVDAAALDAALASGKVRYAAIDADIFVDAETGAITGPMAPYLAVDKKYP
                     GRLELLPHAAADTEHVSRVEGAKQAVDQIIACIRYKEVVNLKGDLPDGYVRGKTQTVG
                     GVGRCSSAELTRALENTEATAEARRTAEAMAAIWGALSSASNAIARNELVERHGATLV
                     RNFNSYATLMRKLGLEGPYD"
     gene            complement(4832..>5114)
                     /locus_tag="AC16A0_10820"
     CDS             complement(4832..>5114)
                     /locus_tag="AC16A0_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105874.1"
                     /translation="PDTSLLATAFSLALAASLIVGARRGSMLVLVLALLSAVLAGLTV
                     WNAFSLRPALWPSEFWRFVAVALNALGIGGAILVFADAARRQLVAQGRR"
BASE COUNT          924 a         1567 c         1602 g         1021 t
ORIGIN      
        1 cggcatgctg ctcgcaattc cgatcggcct ggtgcaggtg actggaccgt cgtggctcgc
       61 cttcatcgcc cgttggttct gcaccatcat ccgcggaacg ccgctgctga ttcagctctg
      121 gctgatctac tacggaatcg gctcgctttt tccgcagatc ggcgccgcat acccgggctt
      181 ccgcgagaac ttcatgtggc tgatacgcct cgattccttc tactatgcgc tgtttgcctt
      241 caacatcaga ttcgcaggct atgagggcga gatcatgcgc ggtgcgttcc tcggcgttcc
      301 gcgcggcgag ctggaggccg cacgcgcttt tggcatgtcg ccctggaagg tactgacacg
      361 cgtatggttc ccgcgcgccg tgcgccttgt gcttcccacc cttgccggcg aactggtgct
      421 gcaactgaag tcgacaccca tcgcctttac cgtcgcggtc atggacctga tgggcgtcac
      481 cacgaagata cgtcaggaca ccttccgcat cttcgagcct ctgatgctgc ttgccgtgat
      541 atacatgtgc ctgacatttg tgattgtctg ggcctttggc ttccttgaac gcatggtgcc
      601 gcagaagcga tgatcgaaga cctcttgatc ccgcatcgga acgaactgac ggcgatccgg
      661 cacgaccttc acatgcaccc ggagatgctt ttcgaagagg tgcggacaag cgccatcgtg
      721 gcgcgcgagc ttgcaaggct tggcttcaag gttacgacag gacttgccaa gaccggcgtg
      781 gtcggaaccc tcagcaacgg cagcagcggc aggtccatcg gcatccgcgc cgacatggac
      841 gcgttgccca tacacgagac cacaaacctg ccctatgcct cgaagtatcc cggcaagatg
      901 catgcctgcg gtcatgacgg gcataccacc atgcttcttg gcctcgcccg gtacctcgct
      961 gaaagccgca acttcgatgg tacggtccac ctcatcttcc agccatcgga ggaggacgag
     1021 ggcggtgccc tgcgcatgat cgaggatggc ttgttcaagc gattcccctg cgatgcggtc
     1081 tttgcgcttc acaacatacc gggcgaggag ccgggtcagg tcatcgtcag gcccggcccg
     1141 atcacggctg ccgtcgacat cgtgggcatc actgtgcggg gacttggtgg ccacggtgcc
     1201 gtcccgcaga aggcgatcga tccgatcgtt gccgcgtcca gcctggtgat cgctctccag
     1261 tccgtgatat cgcgcaacat cgatccgctg gatcctgctg tcatcacggt gggatccttc
     1321 aacgctggct ccatggcgac cgtcattccc ggagaggcga aactcctggt tggtgtaaga
     1381 acctgcacgc gacaggtgcg cgaattgatc ctcaagcgca tcgaggatct ggccaagggg
     1441 caggcgcggt ccttcggctg cgaggcggac gtgcagatcg gcgacggcag ctggagctat
     1501 ccggcgggag caaattccgc ccccgaggcc gccctggttc gggccgtcgc ccttgagatg
     1561 ggacaggatc ctgccaaggt cgacatgcgc ggtcccttca tgttctcgga agactttgcc
     1621 tacatgcagg aactggtgcc atcgtgctac ttcggcatcg gcaatggcag cggcccgatg
     1681 ctccacgact cgagctacaa tttcaatgac gagcttctgg tcaagggccc cgcattctgg
     1741 gcgcggctgg tggccaaggc tctgcctcag tcatagggtc cttcgagtcc cagcttccgc
     1801 ataagggttg cataggaatt gaagttccgc accaaggtcg cgccgtggcg ttcaaccagt
     1861 tcgtttcggg cgatcgcgtt gctggcactc gacaaggcgc cccagatggc agccatggcc
     1921 tccgcggtcc tgcgggcctc ggctgtcgct tcagtgttct caagcgcgcg tgtcagctcg
     1981 gccgaagagc accggccgac tccgccgacc gtttgggtct ttccgcgcac ataaccgtcc
     2041 ggcagatcgc ctttcaagtt gaccacttcc ttgtagcgga tgcaggcgat gatctggtcc
     2101 acggcctgct ttgcgccctc aacgcgggag acatgctcgg tgtcggctgc tgcatgggga
     2161 aggagttcca gtctgccagg gtatttcttg tccacagcaa gatagggcgc catcggaccg
     2221 gtgatcgcgc ctgtctctgc atcgacgaag atatcggcat ctatggccgc ataacggacc
     2281 ttgccgctgg cgagcgcggc atccagtgcg gcagcgtcga cacactcgcc gcggtcgtag
     2341 ttgatcacga tggcgccgtc gttcagttgc gccaggaccg ccgcgttcac cacgttggca
     2401 ttcgcgaatt tgcctgttgc cgcattgagt gggccgaggc cggtgtgggg ggagatgaca
     2461 tctgcgccac gcgcggcgtc ttccggcgtg ggggcgaatt cgaaaccctc ggattcgatc
     2521 cagtccttgt ggcgcggcct ggcatggacg gcaacgcgca tgccgaaagc ctgtgcaagc
     2581 ttggccactt cgcggccgat gtttccatat ccgatgatcg cgatcttctt gccttccagc
     2641 ttctcggtcg ggaattcctt gaggttccgg cccgtgtcaa actgaccagc aatggtgcgg
     2701 cggtgaagct catcgactgg caggtcgggc atgaccttga gcaacgcctt catcgccata
     2761 tgggccgtgg cgcggctgtt gaacgagggg gtattcatca gcggggcaac gccgccttcg
     2821 ccattgccgc cgccccagga ggccgacccc atgttgccgg tacccgcgcc gatgcggacg
     2881 ccgcccagcg gaaagaccgc gtccttggga agaaacgttg cggctgcgat gaccgcatcg
     2941 aactgaccct tgtcggtgag cggcagaatc tctgcagccg tcgatagctg cggcatatag
     3001 aagaagtgga tcttgccctt ggcaagcttc ttcccatctc caggcgggcc cagatggaac
     3061 acgccgccct gagattcgat atgagcctgg acggcgcctg gatcgggctt gcccctggca
     3121 tcgaacttca ggccgatcag gtcgcatatc agtaccctgt aggcgcggtc tggatcatcc
     3181 ttgcgttgcc tggagccaac ctctggggga gccgggaagg tcacaccgag tttggcaagc
     3241 tcttcctcca gcaccacgat cttcgcccgg tgataggcat acttgagatt gtcgagcagg
     3301 gcggtgacat cttcgagggg acggatgccg ccgatccagg cccggtagtc gcccggcgtt
     3361 ccaggaaaag cgttgatgta gcgggcgacg tcaagtcccg cctcgtgctc gccattggga
     3421 tgggtgatgc cgtcataggc caaaagctgc ttcgagcggg cgacgatgcg ggactgaatg
     3481 gccgcatccg cgatgtcggg atccttcacc ttgataagtg tgacggcggc cccccgggct
     3541 tccgggtttt cgacgcccag ctcgaaaagg gaggagttgg acatccactc gttcactgcc
     3601 gcacgattgg caaccgaccg gcgattgagc tccgtgacgt cgccgatctt cctttccacc
     3661 cgcagaagtc cgaaagtcgt ttcggcgaag gcgagggtgg agaatgtgtt gatgaccccg
     3721 ccaagcatct tgtcctgatc gggatcgtag aaggggccca gcttcacgct ccgggtgccc
     3781 gtaagcggct tgcgggcatc ggctggcgct gcaagtttca attggcgcgg aatggcccag
     3841 gaaggggctt tctggttctc ttccaccagc gccagagcat gcggcgtgaa ggagacgatg
     3901 aagtagccgg acacgcctcc gattgccttc tggaagggca tgaagaggca gcacttcgac
     3961 atgacctgtc tcacaatgtc ctcgccccag ggcatggcgc caagcatcga ggtcgcgtcg
     4021 agaacagcat gatgttcgcc agggttgcga tcgatccagt cgagcagttg agtgacttcg
     4081 ccctttgtgt agcaggtggc accagttgtc tcatgaccca cgccgaagaa gaccttgatc
     4141 cccaggcgtt ccaacgtgtc ggaactggga atgcctcctt ccgtctttgc aaagtgtact
     4201 cggccgggat cgccctgccg ggcgtgtgac agcatttcca cgatctgtgc gccccaggac
     4261 tgccggaaga agccagaagc ctttgcctcc tcgctctcgg gggccacggt atcgacatag
     4321 atgtgctggg agggatcgtt gcaggtcagc agatgaagcg cgcagaccgt gaatccggag
     4381 tgtccgcccc ccaggcctac ggccatcttg ttcgatctcg ggaaacggaa gtagcgatgg
     4441 atctcccgca tcatgtcgag caggaacttg tcggccgggt agccgcggtg catggaccgg
     4501 gcaatctcgg ctaccgtgaa ggggccaatg tccttgccgg tatcgtcgag cttgcggtag
     4561 ttcgtttcaa gccagtgttc gaaggccagg cgcgtgagcc ggctgtcggc ctcgtccagg
     4621 gcgccatcgg tgatcgggcc gacgccgaag aacgggtttg acgtgcgcag cggcgccccg
     4681 ccggcgctgg ccttgagtga tgtctcgcgc tgagttttga gcgattcgat cagagtttgc
     4741 tgcatttaac cctcccggat gcggccggac actagatgag ggcggcaggg ccgaatttga
     4801 caaaaacgtc acatatcgga aggagccgcc gctagcgccg cccttgagcc accaattggc
     4861 ggcgggcagc atcggcaaac accaggatgg ctcctccaat ccccagggca ttcagcgcca
     4921 cggccacgaa acgccagaat tccgacggcc agagcgctgg ccggaggctg aaggcgttcc
     4981 agaccgtcag accggcaagc accgcactca gcaaggccag gacgaggacc agcatgcttc
     5041 cacgccgggc cccgacgatc aggcttgcgg ccagggcaag cgagaaggcc gttgccagaa
     5101 gtgatgtatc cggt
//
LOCUS       DBNGVR010000518         3330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3139186, whole genome shotgun sequence.
ACCESSION   DBNGVR010000518 DBNGVR010000000
VERSION     DBNGVR010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3139186"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1973
                     /gene="cysN"
                     /locus_tag="AC16A0_10825"
     CDS             42..1973
                     /gene="cysN"
                     /locus_tag="AC16A0_10825"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HIR3105875.1"
                     /translation="MNQPTTESSYKAQELIAQDIDAYLHQHQHKSLLRFITCGSVDDG
                     KSTLIGRLLYEAKMLFEDQLSALEADSKKMGTQGGNLDFALLVDGLAAEREQGITIDV
                     AYRAFTTDKRKFIVADTPGHEQYTRNMATGASTADLAIILIDARKGVLTQTRRHTFIC
                     TLLGIRHLVVAVNKMDLMDYRKDVFDKIEQDYRAFAKSVGASDITMIPLSALAGDNVI
                     ARSPNMPWYHGSTLMAHLETVPVGDDLDSQPFRLPVQWVNRPDQNFRGFSGSIVSGSV
                     APGDAIKVLPSGKTSTIERVVTYGGDLAKGVAGQSLTVTFRDEIDVSRGDVICAANAP
                     VEVSDQFQVDILWMDEEAMLPGRSYVIKAGTRQVPGTIETIRHKVNVNTMEHVAAKSL
                     ALNEIAVCNLELDTPIAFAPYKENRHLGSFIVIDRFSNNTVGMGLIRFALRRAANIHW
                     QALDVNKVSRAGLAGQKPAVLWFTGLSGAGKSTIANLVEKKLHAEGRMTYLLDGDNVR
                     HGLNRDLGFTDADRVENIRRVAEVAKLMVDAGLIVLVSFISPFRSERLMARELVEDGE
                     FVEIFVDTPLAEAEKRDVKGLYAKARRGEIKHFTGISSAYEAPERPEIRIDTTKLTAE
                     GAADAIVEHLRAAGRLAKA"
     gene            complement(1983..2591)
                     /locus_tag="AC16A0_10830"
     CDS             complement(1983..2591)
                     /locus_tag="AC16A0_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3105876.1"
                     /translation="MTLTFWFDVHSPWAYLAAHRIGALARKHDQALIWKPIHLPRLMD
                     AIDGFKPLEAAPQRVTWFKQDVLDHAELQGLPLRYHPRYPLRNARALRACQHAADGGR
                     GEAFACRVLRAYWSEEGDIEDLDALARWGEESGLDGIAIRAAAVSETCKRKIELNTEA
                     AIARGIFGVPTVDTGSKLYFGSDRLDLLDLHLGRRAGVGHPR"
     gene            complement(2588..>3330)
                     /locus_tag="AC16A0_10835"
     CDS             complement(2588..>3330)
                     /locus_tag="AC16A0_10835"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3105877.1"
                     /translation="RNYDLSSLRGGTCGGADCNPDVLRRCAEDFPMQGLCQVYGQTEG
                     STLFTCPSTEDPLRWETAGTALPGYELRIVDPATLQPLPAGQIGELQGRGPMVMQGYY
                     KKPDDTAETIIAGGWLRSGDLGFFTDTGHLVIAGGRLRDMIIRGGENIYPAEIENLLQ
                     AHPAVAGVAVFGLADTHYGEIVAAAVKLSVPVAAAALQAHCAARIAKFKVPAVIYAVE
                     SFPLTPSGKIRKVDLRQWAAERRMERLA"
BASE COUNT          619 a         1159 c         1016 g          536 t
ORIGIN      
        1 tttctgaacg tcgccccggc gatgtgcgga agcgtttcac catgaatcag ccgaccaccg
       61 aatcctcgta caaggcgcaa gaactcattg cgcaggacat cgacgcctac ctgcaccagc
      121 accagcacaa gtcgctgctg cgcttcatca cctgcggctc ggtcgacgac ggcaagtcga
      181 cgctcatcgg acgattgctc tacgaagcca agatgctctt cgaggatcag ctctcggcgc
      241 ttgaggccga ctccaagaag atgggcaccc aaggcggcaa tctcgacttc gccctgctgg
      301 tcgacgggct tgcggccgag cgcgagcagg gcatcaccat cgacgtcgcc taccgcgcct
      361 tcaccaccga caagcgcaag ttcatcgtcg ccgacacgcc gggccatgag caatacacgc
      421 gcaatatggc gaccggcgcc tccaccgccg atctcgccat cattctcatc gacgcgcgca
      481 agggcgtgct cacccagacg cggcgccaca ccttcatctg caccctgctc ggcatccgcc
      541 atctcgtggt cgcggtcaac aagatggacc tgatggacta ccgcaaggat gtcttcgaca
      601 agatcgagca ggactatcgc gccttcgcca agtcggtcgg cgccagcgac atcaccatga
      661 tcccgctctc ggccctggcc ggcgacaatg tcatcgcgcg atcgcccaac atgccctggt
      721 atcatggctc gacgctgatg gcccatctcg agaccgtgcc ggtgggcgac gacctcgaca
      781 gccagccctt ccgcctgccg gtgcaatggg tcaaccgccc ggatcagaat ttccgcggct
      841 tctcgggtag catcgtgtcc ggcagcgtgg cgccgggcga tgccatcaag gtcttgccat
      901 cgggcaagac atcgaccatc gagcgggtcg tcacctatgg cggcgatctc gccaagggcg
      961 tagccgggca gtcgctcacc gtgaccttca gggacgagat cgacgtcagc cgcggcgacg
     1021 tgatctgcgc tgccaatgcg cccgtcgagg tttccgacca gttccaggtc gacattctct
     1081 ggatggacga ggaagcgatg ctgccggggc gctcctatgt gatcaaggcc ggcacccgcc
     1141 aggttccggg aaccatcgag acgatccggc acaaggtgaa tgtcaacacc atggagcatg
     1201 tggcggccaa gagcctcgcg ctcaacgaga tcgccgtctg caacctcgaa ctcgacacgc
     1261 cgatcgcctt cgcgccctac aaggagaacc gccacctcgg gtccttcatc gtcatcgacc
     1321 gtttctccaa caacacggtc ggcatgggcc tcatccgctt cgctttgcgc cgcgccgcca
     1381 acatccactg gcaggccctc gacgtgaaca aggtgtcgcg cgcaggcctc gcgggccaga
     1441 agcccgccgt cctgtggttc acgggacttt cgggcgccgg caagtcgacc atcgccaatc
     1501 tcgtcgagaa gaagctacac gccgaaggcc gtatgaccta tctgctcgac ggcgacaacg
     1561 tgcgccacgg gctgaaccgc gacctcggct ttaccgatgc cgaccgcgtc gagaacatcc
     1621 gccgcgtcgc cgaagtggcg aaactgatgg tcgacgccgg actgatcgtg ctggtctcct
     1681 tcatctcgcc cttccgctcc gagcgcctca tggcgcgcga actggtcgag gacggcgagt
     1741 tcgtcgagat cttcgtcgac acgccgctgg ccgaggccga gaagcgcgac gtgaaaggcc
     1801 tctacgccaa ggcgcggcgc ggcgagatca agcacttcac cggcatctcc tcggcctacg
     1861 aggcgccgga acggcccgag atccgcatcg acacgacgaa gctgaccgcc gaaggcgcag
     1921 ccgatgcgat cgtcgaacac ctgcgcgccg ccggccgcct ggcgaaggct tagaccgtcc
     1981 cgtcagcgcg ggtggccaac accggcacgc cgaccgaggt gcaggtcgag gagatcaagc
     2041 cggtcgctgc cgaagtagag cttgctgccg gtgtcgacgg tcggcacgcc gaagataccg
     2101 cgggcgatcg ccgcctcggt attgagttcg atcttccgtt tgcaggtctc gctcaccgcg
     2161 gcagcgcgaa tggcgatgcc gtcgagcccg ctctcctcgc cccagcgcgc cagcgcatcg
     2221 agatcctcga tgtcgccctc ctctgaccag taggcgcgca gcacccggca ggcgaaggcc
     2281 tcgccgcggc ccccgtcggc agcatgctgg caggcgcgaa gcgcccgcgc attgcgcagc
     2341 ggatagcgcg gatgatagcg gagcggcagg ccctgcaatt cggcatggtc gagaacgtcc
     2401 tgcttgaacc aggtcacgcg ctgcggcgcc gcctcgagcg gcttgaagcc atcgatggcg
     2461 tccatgagcc gcggcagatg gatcggcttc cagatcaatg cctgatcgtg tttgcgcgcc
     2521 agcgcgccga tgcggtgtgc ggcgagatag gcccagggcg aatgcacatc gaaccagaag
     2581 gtcagcgtca tgccagccgc tccatgcgtc gctccgccgc ccactggcgc agatccacct
     2641 tgcggatctt gcctgacggc gtgagcggaa aggattcgac cgcgtagatc accgccggca
     2701 ccttgaactt cgcgatgcgc gccgcgcaat gggcctgcag cgccgctgcg gcgaccggca
     2761 ccgaaagctt caccgctgcc gcgacgatct cgccataatg cgtatccgca agaccaaaga
     2821 ccgcgacacc cgcaacggcc ggatgggcct gcagcagatt ctcgatctcg gccggataga
     2881 tgttctcgcc gccgcggatg atcatgtcgc ggagacgtcc gcccgcgatg acgagatggc
     2941 ctgtgtcggt gaagaaaccg agatcgccgc tgcgcagcca gccgcccgcg atgatggtct
     3001 ccgccgtatc gtcaggtttc ttatagtagc cctgcatcac catcggcccg cgcccctgca
     3061 attcaccgat ctgtcccgca ggaagcggct gtagcgtcgc cggatcgacg atgcggagct
     3121 cgtaacccgg aagcgccgtt cccgcggtct cccagcgcag cggatcttcc gtggacggac
     3181 aggtaaacag cgtcgaccct tccgtctggc catagacctg acagaggccc tgcatcggaa
     3241 aatcttcggc gcagcgccgc agcacgtccg gattgcagtc cgcgccgccg caggtgccgc
     3301 cgcgcagcga cgacagatcg taattgcgcc
//
LOCUS       DBNGVR010000519         4324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_3730369, whole genome shotgun sequence.
ACCESSION   DBNGVR010000519 DBNGVR010000000
VERSION     DBNGVR010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_3730369"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16A0_10840"
     CDS             complement(<1..240)
                     /locus_tag="AC16A0_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012576.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3105878.1"
                     /translation="MPTMKDVAKAAGVSIATVSATLSGASYVSPELQARVSAAIAALG
                     YERNSMASGLKRGRSSLIGLIVSDVTNPFFTELVDH"
     gene            complement(243..884)
                     /locus_tag="AC16A0_10845"
     CDS             complement(243..884)
                     /locus_tag="AC16A0_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008534538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HIR3105879.1"
                     /translation="MSQARPTATRKTRIQAQNEELILAAALDVFSSLGYRGSTVDQIA
                     AKCGLSKPNLLYYFRRKEDIYFAVLEKILAEWGDTLKSLDPQGDPIEEIGSYIRQKLR
                     LSRDNPRASRLFANEILQGGAAIGDFLKGPVRVLLHEKAAVIAEWVREGRINPIDPYH
                     LIIAIWATTQHYADFDVQLRAVLDERERSLEAAERTLIALFCDGLRPRLPGSQ"
     gene            967..1746
                     /locus_tag="AC16A0_10850"
     CDS             967..1746
                     /locus_tag="AC16A0_10850"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3105880.1"
                     /translation="MTAAAVIEARDVSLTFQTADGPVYALSKVNLTVTEGEFVSFIGP
                     SGCGKTTLLRVIADLEQATDGRISVNGMSPQDARLKRAYGYVFQAPALYPWRTVERNV
                     GLPLEIMGLDDAAGRIRRNLELVNLAGFEKKFPWQLSGGMQQRVSIARALAFDPKLLL
                     MDEPFGALDEIVRDKLNQQLLELWDRTGKTVVFVTHSIPEAVFLSTRIVVMSPRPGRI
                     IDIIETNFPRNRTLDMRETPEFLAVAHRVREGLKAGHSYED"
     gene            1736..2638
                     /locus_tag="AC16A0_10855"
     CDS             1736..2638
                     /locus_tag="AC16A0_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105881.1"
                     /translation="MKTEAAAVPSLPARGEGNAFPVAVICLVILVVWYIAAIPMNWVV
                     TESKIAAAGGGAADILYYSWNEKRPVIPAPHQIVMELWNAVFMVKPWAVKSLLYHGWV
                     TLSSTLLGFVLGSLFGIALAIAIVHSTFLSKSLMPWIIASQTIPILAIAPMVIVVLGS
                     LGFTGLLPKSLISMYLCFFPVTIGMVKGLTSPDPMQLDLMQTYNSSASDVLWKLRFPS
                     SVPFLFTSLKFAVAIALTGAIVGELPTGAIAGIGARLLQGSYYGQTVQIWAALFMGSF
                     LAAGLVWLVGVAERIVLKRMGARP"
     gene            2635..3405
                     /locus_tag="AC16A0_10860"
     CDS             2635..3405
                     /locus_tag="AC16A0_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3105882.1"
                     /translation="MKRFQATDLIYPALFGISVLVLWQLIVKGFNVPAVLLPAPSDIW
                     AKLITSLPILWVDFVQTVLRAVIPGWIIGSLSGFLVAILCDRIPFLRRGLLPLGNLVA
                     ALPIVGVAPILVMWFGFDWPSKAAMVVVMTFFPMLVNAVAGLQASGHLERDLMQSYGA
                     SWWQTLVKLRLPAAMPFIFNALKINSTLAMIGAIVAEFFGTPIVGMGFRISTEVGRLG
                     LDMVWAEIAVAALTGSAFYGLLVALERVVTFWHPSYRR"
     gene            3444..>4324
                     /locus_tag="AC16A0_10865"
     CDS             3444..>4324
                     /locus_tag="AC16A0_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3105883.1"
                     /translation="MKKFLMAAAAAGALLVSGTSAWAEAVKLQLKWVTQAQFAGYYVA
                     AAKGFYKDAGLDVTIMPGGPDINPQQVLAGGGADVVVDWMPSALATREKGSPVVNIAQ
                     PFKKSGMMLTCRKETGITKPEDFKGKTLGVWFFGNEYPFLSWMSTLGIPTTGGADGVT
                     VIKQGFNVDPLIQKQADCISTMTYNEYWQVIDAGIPADQLVVFKYEDQGVATLEDGLY
                     VLEDKLKDPAFVDTMAKFVAASMKGWDYARANGDEAAQIVIDNDASGAQTIEHQKRMM
                     GEINKLTEGSDGTLDVAA"
BASE COUNT          739 a         1382 c         1345 g          858 t
ORIGIN      
        1 gtgatcgacg agttcggtga agaagggatt ggtgacgtcc gagacgatga gtccgatcag
       61 cgaggagcgt ccgcgcttca gaccgctggc catggaattg cgttcatagc cgagcgccgc
      121 aatcgctgca ctcacgcgcg cctgcagttc cgggctcaca taggaggcgc cggacaaggt
      181 cgccgagacc gtggcgatgc tcacgcccgc cgccttcgcc acatccttca tggttggcat
      241 gatcactgcg atcctggaag tcgcggccgc aagccgtcgc aaaacagcgc gatgagggtg
      301 cgttcggctg cctccagcga cctttcgcgc tcgtccagca cggcgcgcag ctgaacgtcg
      361 aaatcggcat agtgctgggt cgtggcccag attgcgatga tgagatggta ggggtcgatc
      421 ggattgatcc ggccctcccg gacccactct gcgatgacgg cagccttctc gtgcagaagc
      481 acgcggaccg gacccttgag aaagtcacct atggctgcgc cgccctgcag gatctcgttg
      541 gcgaagagcc gcgaggcgcg cgggttgtcg cgcgacaggc gcagcttttg cctgatgtag
      601 gacccgatct cctcgatcgg gtctccttgt ggatcgagcg acttcagcgt gtcgccccac
      661 tcggcgagga tcttctcgag aacggcaaaa tagatgtctt ccttgcggcg gaaataatag
      721 agaagattgg gtttggacag gccgcatttt gcggcgatct gatcgaccgt cgatccgcgg
      781 tagccaagcg atgagaagac atccagcgcg gcggcaagga tcagctcttc attctgcgcc
      841 tgaattctgg tcttgcgcgt cgccgtcggt ctcgcctgtg acatgccgtc actttctaaa
      901 ttcttgatcg gctggtcaag tacggatagc gtttcccgaa acaataagaa gaacggggga
      961 gcgcgcatga cggctgcagc cgtgatcgag gccagagacg tgagtctgac ctttcagact
     1021 gccgacggtc cggtctatgc cctgtcgaag gtcaatctca cggtaacgga aggtgagttc
     1081 gtctccttca tcggtccctc cggttgcggc aagaccacgc tgctgcgcgt catcgccgat
     1141 ctggagcagg ccaccgacgg gcggatcagt gtcaatggca tgagcccgca ggacgcgcgg
     1201 ctcaagcgcg cctatggcta tgtgtttcag gcacccgcgc tctatccctg gcgcacggtg
     1261 gagcgcaatg tcggcctgcc gctcgagatc atggggctcg acgatgccgc cggccgcatc
     1321 cgccgcaatc tggaactggt caacctggcg ggtttcgaga agaagtttcc ctggcagctc
     1381 tcgggcggca tgcagcagcg cgtctccatc gcccgcgcgc tggccttcga tcccaagctg
     1441 ctgctgatgg acgaaccctt cggcgcgctc gacgagatcg tgcgcgacaa gctgaaccag
     1501 cagctcctgg aattgtggga tcgcaccggc aagaccgtgg tcttcgtgac ccattccatc
     1561 cctgaagccg tgttcctgtc caccaggatc gtggtgatga gcccgaggcc cggccgcatc
     1621 atcgacatca tcgagacgaa cttcccgcgc aaccggacgc tcgacatgcg cgagacgcca
     1681 gagttcctcg ccgtcgctca ccgggtccgc gagggtctca aggccgggca ttcctatgaa
     1741 gactgaagcc gccgcggtgc catccttgcc ggcacggggt gaaggcaatg ccttccccgt
     1801 cgcggtcatc tgcctcgtca tcctggtcgt ctggtacatc gccgccatcc cgatgaactg
     1861 ggtggtcacc gagtccaaga tcgcggcggc cggcggtggc gcggcggata tcctctacta
     1921 ctcgtggaac gagaagcgtc cggtcattcc ggcaccgcat cagatcgtca tggaactgtg
     1981 gaatgccgtc ttcatggtca agccctgggc ggtgaagtcg ctgctctatc acggctgggt
     2041 gacgctctcc tccacgctgc ttggcttcgt gctgggctcg ctcttcggca tcgcgctggc
     2101 cattgccatc gtgcattcga ccttcctgtc caagagcctc atgccctgga tcatcgcgtc
     2161 gcagaccatt ccgatcctgg ccattgcgcc catggtcatt gtggtgctgg gctcgctggg
     2221 attcaccggg ctgctgccca agtcgctcat ctcgatgtat ctctgcttct tcccggtcac
     2281 catcggcatg gtgaaagggc tcacctcgcc cgatccgatg cagctcgacc tgatgcagac
     2341 ctacaattcc agcgcttcgg acgttctgtg gaagctgcgg ttcccgtcct ccgtgccctt
     2401 cctgttcacg tcgctgaagt tcgcggtggc catcgcgctc accggggcga tcgtcggcga
     2461 actgcccacc ggcgccatcg ccggaatcgg cgcgcgcctg ctgcagggct cttattatgg
     2521 ccagacggta cagatctggg cggcgctctt catgggctcg ttccttgccg ctggcctggt
     2581 ctggctggtg ggcgtggccg aacgcatcgt gctgaagcgc atgggggccc ggccatgaag
     2641 cgctttcagg cgacagacct catctatccg gcgctcttcg ggatttccgt gctggtcctc
     2701 tggcagctga tcgtgaaggg tttcaacgtt ccggccgtgc tgctgccggc cccgtccgac
     2761 atctgggcca agctgatcac gtcgctgccc atcctctggg tggatttcgt ccagaccgtg
     2821 ctccgtgccg tcattcccgg ctggatcatc ggctcgctct cgggtttcct ggtggccatt
     2881 ctctgcgacc gcatcccttt cctgcgccgc gggcttctgc cgctgggcaa tctggtggcg
     2941 gcacttccca tcgtcggcgt ggcgcccatc ctggtcatgt ggttcggctt cgactggccc
     3001 tccaaggccg ccatggtcgt ggtcatgacc ttcttcccca tgctggtgaa tgccgttgcg
     3061 gggttgcagg cctcgggcca cctggaacgc gacctgatgc agagctacgg cgcatcctgg
     3121 tggcagacgc tggtcaagct gcgccttccg gccgccatgc ccttcatttt caacgccctg
     3181 aagatcaact cgactctcgc catgattggg gccatcgtcg ccgagttttt cggcacaccc
     3241 atcgtcggca tgggctttcg catttccacg gaagttggca gactggggct ggacatggtc
     3301 tgggccgaga tcgcggtggc agcactgacg ggctcggcct tctacggtct ccttgtcgct
     3361 ctcgagcgcg tggtgacctt ttggcatccg tcctaccgca ggtaggcggc acgacagaaa
     3421 gaagaacaga ggagagagac aggatgaaga aatttctgat ggcggccgcg gccgccgggg
     3481 cgcttctggt cagcggaacc tccgcctggg ccgaggctgt caagctgcag ctcaagtggg
     3541 tgacccaggc gcagttcgcc ggctactacg tcgccgcggc aaagggcttc tacaaggacg
     3601 ccggcctcga cgtcaccatc atgccgggcg gtcccgacat caacccgcag caggtgctgg
     3661 ccgggggcgg cgccgatgtg gtggtcgact ggatgccgtc cgcgctggcc acgcgcgaga
     3721 agggctcgcc cgtcgtcaac atcgcccagc ccttcaagaa gtcgggcatg atgctcacct
     3781 gccgcaagga aaccggcatc accaagccgg aagacttcaa gggcaagacg ctgggcgtct
     3841 ggttcttcgg caacgaatat cccttccttt cctggatgag cacgctcggc attccaacca
     3901 ccggcggcgc cgatggcgtg accgtgatca agcagggctt caacgtcgac ccgctgatcc
     3961 agaagcaggc cgactgcatc tcgaccatga cctacaacga atactggcag gtcatcgacg
     4021 ccggcatccc ggccgaccag ctggtggtct tcaagtacga ggaccagggc gtggccacgc
     4081 tcgaggatgg cctctatgtg cttgaagaca agctcaagga tccggccttc gtcgacacca
     4141 tggccaagtt cgtcgcggcc tcgatgaagg gctgggacta cgcccgcgcc aatggcgacg
     4201 aggcggccca gatcgtcatc gacaatgacg cctcgggcgc ccagaccatc gagcatcaga
     4261 agcgcatgat gggcgagatc aacaagctca ccgaaggctc cgacggcacg ctcgacgtgg
     4321 ccgc
//
LOCUS       DBNGVR010000520         3455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2945100, whole genome shotgun sequence.
ACCESSION   DBNGVR010000520 DBNGVR010000000
VERSION     DBNGVR010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2945100"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /gene="zapE"
                     /locus_tag="AC16A0_10870"
     CDS             complement(<1..404)
                     /gene="zapE"
                     /locus_tag="AC16A0_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015902.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HIR3105884.1"
                     /translation="MPELLDLYRTAAARGDLRSDTAQETVLARLAVLSDALAASTPRG
                     LLGRFRRPAERLKGLYIFGEVGRGKTMLMDLFFAHAAIASKRRVHFHAFMQDVHARLH
                     ALRRNQAQDAIAPVAKAIAAESRLLCLDEMQVT"
     gene            complement(409..1635)
                     /locus_tag="AC16A0_10875"
     CDS             complement(409..1635)
                     /locus_tag="AC16A0_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease"
                     /protein_id="HIR3105885.1"
                     /translation="MQDIFQNVGRELPFILLLLALLGFRWRVTPRASVFIRAPMDKIF
                     PLVDFSEGDNQRWQRTRVTCTRIDAPGEVYRLTFVTALATGSVQSSNALFEVVKRDAP
                     RLIEVRRVGLEGKSDHSELLRMRTELSPERDGTRFRITYEWGPRPLLAQILGRADLWG
                     SAYRIKGVAETGVPDYRTDALISAGLALVTGAISLWAFAFAFGWGIALLLLAALLVHE
                     FGHLLAYRLIGQPWGRMVFLPFLGAVAVPRLGFVTQGQSVFAAMMGPAFSILIPLAAV
                     AYLMSGPSGIGRDLALSFGLVAVGLNLFNLLPVEPLDGGVILRSVAARLLGSWARFGL
                     IAAGLAILAAGWYAQQVLLLIFGALAILMNIRTRKIDTGLEPLSRLQVVISAFAFMSV
                     VAAYTVFMRFFLENLS"
     gene            1721..2017
                     /locus_tag="AC16A0_10880"
     CDS             1721..2017
                     /locus_tag="AC16A0_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105886.1"
                     /translation="MSNPVFELVIYKVKNPQTAARIRAGLQPHVAAHPGFRDWRAIAS
                     TDDAALFADIVTWDSLDAARAAGQKVMTDPACAPFMAEIGEIFSMGHFAEERRA"
     gene            complement(2014..2229)
                     /locus_tag="AC16A0_10885"
     CDS             complement(2014..2229)
                     /locus_tag="AC16A0_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HIR3105887.1"
                     /translation="MGDAKDCNGNILDDGDSVTVIKDLKVRGAGETLKRGTLIKNIRL
                     TDDPGEIEGRTGSIKGLVLKTAFVKKA"
     gene            complement(2229..>3455)
                     /locus_tag="AC16A0_10890"
     CDS             complement(2229..>3455)
                     /locus_tag="AC16A0_10890"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3105888.1"
                     /translation="HGKTIPDAKGDVQRGLEVVEVCMGAPAMLKGEMMDSAGPGIDLF
                     AMRQPLGVVAGITPFNFPAMIPLWKMAPALSCGNAMILKPSERDPSCPMRLAELFLEA
                     GLPPGILNVVNGDKESVDAILDDPDIAAVGFVGSTPIAQYVYARATAHGKRAQCFGGA
                     KNHMIIMPDADMDKAVDALIGAGYGSAGERCMAISVAVPVGESTANALVEKLVPRVEK
                     LKVGLSTDREADYGPLVTKDALARVKGYIDVGVKEGATLKVDGRGFKMQGYEKGYFLG
                     GSLFDNVTKDMRIYKEEIFGPVLSVVRAKTYDEGLALVNDHEYGNGTAIFTRDGDTAR
                     DFAARCQIGMVGINVPIPVPLAYHTFGGWKRSSFGDLNQHGPDSVRFYTKTKTVTARW
                     PSGIKEGAQFSIPTMR"
BASE COUNT          667 a         1121 c         1112 g          555 t
ORIGIN      
        1 gtcacctgca tttcatcaag gcagagcagc cgcgactccg cggcgatcgc ctttgccacc
       61 ggcgcgatgg catcctgcgc ctggttccgc cgcagggcat ggagccgggc atggacatcc
      121 tgcatgaagg catgaaaatg aacgcgccgc ttgctggcga tggcggcatg cgcgaagaag
      181 agatccatca gcatggtctt gccgcggccg acctcgccaa aaatgtagag gcccttgagc
      241 ctttcggcag gcctgcggaa gcggccaagc aggccgcgcg gcgtgctggc cgctagcgca
      301 tcggaaagga ccgccaaacg ggccagcacc gtttcctgcg cggtatcgct gcgcaggtca
      361 ccgcgcgcgg cagcggtccg gtagaggtcg agaagttcgg gcatcgcctc acgagaggtt
      421 ttcgaggaag aagcgcatga acaccgtgta ggccgcaacc accgacatga aggcgaaggc
      481 ggaaatcacc acctggagcc gcgacagcgg ttcgaggccc gtatcgatct tccgtgtacg
      541 gatattcatc aggatggcca aggctccgaa gatcagcagc aggacctgct gggcatacca
      601 tccggcggcc aggatggcga ggcccgcggc aatgagcccg aaacgggccc agctgccgag
      661 cagccgcgca gccaccgaac gcaggatcac gccgccgtca agcggctcga ccggcaacag
      721 gttgaagaga ttgagcccga ccgccaccaa tccaaagctc agggccaggt cgcggccaat
      781 gccgctcggc ccggacatca gataggcaac ggccgccagc ggaatgagga tgctgaaggc
      841 tggccccatc atggcggcaa agacgctctg gccctgggtg acgaagccca gtcgcggcac
      901 ggcgacggcg ccaaggaagg gcaggaacac catgcggccc cagggttggc cgatgagccg
      961 ataggccagc aggtgtccga actcgtggac cagcagggcg gccagcagca gcagggcaat
     1021 gccccagccg aaggcgaagg cgaaggccca gagcgagatg gcgccggtga ccagcgccag
     1081 gcccgccgaa atcagcgcat cggtgcgata gtccggcacg ccggtctcgg ccacgccctt
     1141 gatgcggtag gcgctgcccc agagatcggc gcggcccagg atctgggcca gaagcggccg
     1201 cggcccccac tcgtaggtga tgcggaagcg cgtaccatcg cgttccggcg acagttccgt
     1261 ccgcatccgc agcagttcgc tatggtcaga cttgccctcc aggcccacgc ggcgcacctc
     1321 aatcaggcgc ggcgcatcgc gcttcaccac ttcgaaaagg gcattcgacg actgcaccga
     1381 tccggtggcg agagcggtca cgaaggtcag gcggtagacc tccccaggcg catcgatccg
     1441 ggtgcaggtg acacgggtgc gctgccagcg ctggttgtcc ccttcgctga agtcgaccag
     1501 cggaaagatc ttgtccatgg gcgcgcgaat gaagacggag gcccgcggcg tcacccgcca
     1561 gcggaagccc agaagggcca gcagcaacag aatgaacgga agctcgcgcc ccacgttctg
     1621 aaagatatct tgcatcgtcc cagccacccc caaggtgctg acatcaactg tcagtctcct
     1681 agcctaaagc agaatcccta cagcaaagtg aggatccttc atgtcaaacc ccgttttcga
     1741 gcttgtcatc tacaaggtga agaatccgca aactgcggcc aggatcaggg ccggactgca
     1801 gccgcatgtt gccgctcacc ccgggtttag ggactggagg gccatcgctt ccaccgacga
     1861 tgcggccctc tttgccgata tcgtgacctg ggacagcctc gacgccgcca gggcggccgg
     1921 ccagaaggtc atgaccgatc cagcctgcgc acccttcatg gccgaaatcg gcgagatctt
     1981 cagcatgggg cattttgccg aggaaagaag agcttaagcc ttcttgacga aggccgtctt
     2041 caacaccagc cccttgatcg aaccggtgcg gccttcgatc tcgccggggt catccgtcaa
     2101 ccggatgttc ttgatcagcg tgccgcgctt cagcgtctcg ccggcacccc tgactttgag
     2161 atccttgatg acggtgaccg agtcaccgtc atcaaggatg ttgccgttgc agtccttggc
     2221 gtcgcccatc accgcatcgt cgggatggag aactgcgcgc cttccttgat gccgctgggc
     2281 cagcgggccg tcaccgtctt ggtcttggtg tagaagcgca ccgagtcggg cccgtgctgg
     2341 ttgagatcgc cgaaggacga gcgcttccag ccgccgaagg tgtgataggc gaggggcacc
     2401 ggaatcggca cgttgatgcc caccatgccg atctggcagc gggccgcgaa gtcgcgcgcg
     2461 gtgtcgccgt cacgggtgaa gatggcggtg ccgttgccgt attcgtgatc gttgaccagc
     2521 gccaggccct cgtcataggt cttggcgcgt accaccgaga gcaccgggcc gaagatctct
     2581 tccttgtaga tccgcatgtc cttcgtgaca ttgtcgaaga gcgagccgcc gaggaaatag
     2641 cctttttcgt agccctgcat cttgaagccg cggccatcca ccttgagcgt ggcgccttcc
     2701 ttgacgccca cgtcgatgta gcccttgacg cgggccagcg cgtccttggt gacaagcggg
     2761 ccgtagtcgg cctcgcggtc ggtggagagg cccaccttga gcttctcgac gcgcggcacc
     2821 agcttctcga ccagcgcatt ggcggtcgac tcgcccaccg gcacggcgac cgagatcgcc
     2881 atgcagcgct cgccggcgga gccgtagccc gcgccgatca gcgcatccac cgccttgtcc
     2941 atgtcggcgt cgggcatgat gatcatgtgg ttcttggcgc cgccgaagca ttgcgcccgc
     3001 ttgccgtgcg cggtggcgcg cgcatagaca tactgcgcga tgggcgtgga tccgacaaag
     3061 cctaccgcgg caatgtccgg atcgtcgagg atggcgtcga ccgattcctt gtcgccgttc
     3121 acgacattga ggatacccgg cggaagtccg gcctcgagga agagttccgc caggcgcatc
     3181 gggcaggacg gatcgcgctc cgagggcttc aggatcatcg cgttgccgca ggacagcgcc
     3241 ggagccatct tccacagggg gatcatcgcc gggaagttga agggcgtgat gccagcaacg
     3301 acacccaaag gctggcgcat ggcgaaaagg tcgatgcccg gacccgccga atccatcatc
     3361 tcgcccttca gcattgcagg cgcgcccatg cagacctcga cgacctcaag cccgcgctgc
     3421 acgtcgccct tggcgtccgg aatggtcttg ccatg
//
LOCUS       DBNGVR010000521         1819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Aestuariivirga sp. isolate MAG05404
            SRR25158526_k127_2357078, whole genome shotgun sequence.
ACCESSION   DBNGVR010000521 DBNGVR010000000
VERSION     DBNGVR010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414812
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aestuariivirga sp. (soil metagenome)
  ORGANISM  Aestuariivirga sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Aestuariivirgaceae; Aestuariivirga.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,171
            Genes (coding)                    :: 2,156
            CDSs (with protein)               :: 2,156
            Genes (RNA)                       :: 10
            tRNAs                             :: 8
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 5 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Aestuariivirga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158526_k127_2357078"
                     /isolate="MAG05404"
                     /isolation_source="soil"
                     /db_xref="taxon:2650926"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC16A0_10895"
     CDS             complement(<1..320)
                     /locus_tag="AC16A0_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3105889.1"
                     /translation="MTKTPDLTGYDPALPVSARIRGRVEAAGRRYHANDNIAEFIAPG
                     ELDKLLDEVSGKLEGVLESLVIDIRNDHNTRDTARRVAKMYLKEVFQGRYVPQPPVTE
                     FPNA"
     gene            complement(464..967)
                     /locus_tag="AC16A0_10900"
     CDS             complement(464..967)
                     /locus_tag="AC16A0_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HIR3105890.1"
                     /translation="MIIEDQSRPFPLSALGTRWQVLTDQVMGGVSRGQLTREIVDGRE
                     ALRLRGDVSLANNGGFVQMALDLSPGGAAIDASQWDGIEMTVRGADETYGLHLRTADL
                     LRPWESYRQGFRATSAWQTLRLPFSGFAAHRTEIPLDLRRLRRLGLVAIGRAFAADLA
                     LAHLRLV"
     gene            complement(964..1812)
                     /locus_tag="AC16A0_10905"
     CDS             complement(964..1812)
                     /locus_tag="AC16A0_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3105891.1"
                     /translation="MTHSALRPDSVAVITGGAAGIGLAAARRFAAAGLKVCIADLGAE
                     RLATAAAGLAATSPGGMNTVMTMEADVSRAEDVARLKAAVDARFGGTDILMNNAGIQP
                     GSAMFGATENWERVLAVNLWGVIHGCQAFVPGMIARGRPGLVINTGSKQGITTPPGDP
                     AYNVSKAGVKVFTEALAHELRNTPDCRLTAHLMIPGFVFTGLTAKGRTEKPAGAWTPE
                     QTVDFMLDRIDAGDFYILCPDNEVTRELDEKRILWAAQDIIENRPPLSRWHPDFGPRF
                     ADFVAK"
BASE COUNT          297 a          593 c          612 g          317 t
ORIGIN      
        1 tcggcattgg ggaactcggt caccggcggc tgcggaacat agcggccctg gaagacctcc
       61 ttcagataca tcttggcgac gcgccgcgcc gtgtcgcgcg tgttgtggtc gttcctgatg
      121 tcgatcacaa ggctttcgag aacgccttcg agcttgcccg atacctcatc gagaagcttg
      181 tccagttcgc ccggcgcgat gaattccgcg atgttgtcgt tggcgtgata gcgccgcccg
      241 gcggcttcca cccgtccgcg aatgcgggca gagaccggaa gcgcaggatc atagcctgtc
      301 agatcggggg tcttggtcat ggtactcgaa ctcgcttgct ggcccgctgt ctgtgtcttt
      361 ccggcatccg tgtatcgcac agccgcttgg cggcgtccaa tccggacagt tgcgagggcc
      421 tttcgctgca aacgaaggct gcgtggactt ggatcaacgg cgctcagaca agccggaggt
      481 gcgccagagc gagatcggcg gcaaaggccc gtccgatggc caccagcccc agccgccgca
      541 ggcgccggag atcgagcggt atctccgtgc ggtgcgctgc gaaccccgag aagggcaggc
      601 gcagggtctg ccacgcagaa gtggcacgaa aaccctgccg gtaggattcc catggccgca
      661 ggaggtcggc cgtgcgcagg tgcagtccat aggtttcgtc cgcgccgcgc acggtcatct
      721 cgatgccatc ccattgcgac gcatcgatgg ccgcgccgcc cggcgacagg tcgagcgcca
      781 tctgcacgaa gccgccgttg ttcgcgaggc tgacatcgcc gcgcaggcgc agggcctcgc
      841 ggccatcgac gatctcgcgc gtcaattggc cccgcgagac gccacccatc acctggtccg
      901 tcagcacctg ccagcgcgtg ccgagcgcag aaaggggaaa gggccgcgac tggtcctcga
      961 tgatcatttc gccacgaaat cggcaaagcg cgggccaaaa tcgggatgcc agcgcgacag
     1021 cggcggacgg ttctcgatga tgtcctgcgc tgcccagaga atgcgcttct cgtccaattc
     1081 gcgcgtcacc tcgttgtcgg ggcagaggat gtagaagtcg cccgcgtcga tgcggtccag
     1141 catgaagtcc accgtctgct ccggtgtcca ggctcccgcc ggcttctcgg tccggccctt
     1201 cgcggtgagg ccggtgaaga cgaagcccgg aatcatcaga tgcgcggtga gccggcagtc
     1261 cggcgtgttg cggagctcat gggcgagcgc ctcggtgaag accttcacgc cggccttgga
     1321 gacattgtag gccggatcgc ccggtggcgt ggtgattccc tgtttcgatc cggtgttgat
     1381 cacgagtccg gggcggccgc gcgcgatcat gccgggcacg aaggcctggc agccatggat
     1441 cacgccccag agattgacgg caagaaccct ctcccagttt tccgtcgcac cgaacatcgc
     1501 actgcccggc tggatgccgg cattgttcat gaggatgtcg gtgccgccga agcgcgcgtc
     1561 caccgcggcc ttgaggcgcg ccacatcctc tgcacggctg acgtctgcct ccatcgtcat
     1621 gacggtgttc atgccgccgg ggctcgtcgc ggcgagaccg gctgcggcgg tggcgagccg
     1681 ttcggccccc agatcggcga tgcagacctt gagtcctgcg gccgcgaagc gccgtgcggc
     1741 ggcaaggccg atcccggccg caccgccggt gatcacggcg actgaatccg gcctgagggc
     1801 ggaatgggtc attgtcggt
//
